Research activity information

• Sep. 2015 日本動物学会, 成茂動物科学振興賞, ミツバチの社会性行動に関する生物学的および数理学的研究：行動誤差の進化的意義の発見

  OKADA RYUICHI

  International academic award


• Jul. 2006 日本比較生理生化学会, 吉田奨励賞, 運動制御における昆虫の脳の基本設計

  OKADA RYUICHI

  International academic award

• Antennal Responses to Volatiles Related to Host Location in the Ambrosia Beetle Platypus quercivorus (Murayama)

  Ryuichi Okada, Yasuto Ito, Michimasa Yamasaki

  Apr. 2025, JOURNAL OF CHEMICAL ECOLOGY, 51(2) (2), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A potential evolutionary trap for the extended phenotype of a nematomorph parasite

  Yuna Sawada, Nozomu Sato, Takeshi Osawa, Kazuma Matsumoto, Ming-Chung Chiu, Ryuichi Okada, Midori Sakura, Takuya Sato

  Abstract Human activities introduce new environmental cues to wild organisms, leading to maladaptive behavioral and life history decisions known as the “evolutionary trap.” This trap is thought to be a major conservation concern for free-living organisms. However, it has never been studied in endosymbionts, one of the most successful and diverse life forms on Earth. Here, we examine this trap in the extended phenotype of a parasite that exploits the visual system of hosts to alter host behavior for its benefit. Arboreal mantids infected by nematomorph parasites are drawn to horizontally polarized light, thereby inducing them to enter the water. In this study, we found that the degree of linear polarization (DOP) of reflected light served as a reliable environmental cue for identifying perennial waters, where nematomorphs can survive in their aquatic life stage without drying out. Infected mantids exhibit attraction to horizontally polarized light with higher DOP in behavioral assays and jumped into pools reflecting light with higher DOP in field experiments. The asphalt road reflected horizontally polarized light closely resembling the polarization levels observed in perennial waters, likely leading to a higher prevalence of mantids on asphalt roads compared with those found in natural arboreal habitats. In a field experiment, we observed infected mantids walking on asphalt roads more often than on cement roads. These findings imply that evolutionary traps can endanger endosymbionts beyond their hosts that directly perceive environmental cues.

  Oxford University Press (OUP), Oct. 2024, PNAS Nexus, 3(10) (10)

  [Refereed]

  Scientific journal


• Massive horizontal gene transfer and the evolution of nematomorph-driven behavioral manipulation of mantids

  Tappei Mishina, Ming-Chung Chiu, Yasuyuki Hashiguchi, Sayumi Oishi, Atsunari Sasaki, Ryuichi Okada, Hironobu Uchiyama, Takeshi Sasaki, Midori Sakura, Hirohiko Takeshima, Takuya Sato

  Elsevier BV, Nov. 2023, Current Biology, 33(22) (22), 4988 - 4994.e5

  [Refereed]

  Scientific journal


• Honey Bee Waggle Dance as a Model of Swarm Intelligence

  Ryuichi Okada, Hidetoshi Ikeno, Hitoshi Aonuma, Midori Sakura, Etsuro Ito

  Aug. 2023, Journal of Robotics and Mechatronics

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Visual learning in tethered bees modifies flight orientation and is impaired by epinastine

  Norihiro Kobayashi, Yuji Hasegawa, Ryuichi Okada, Midori Sakura

  Abstract Visual-orientation learning of a tethered flying bee was investigated using a flight simulator and a novel protocol in which orientation preference toward trained visual targets was assessed in tests performed before and after appetitive conditioning. Either a blue or a green rectangle (conditioned stimulus, CS) was associated with 30% sucrose solution (unconditioned stimulus, US), whereas the other rectangle was not paired with US. Bees were tested in a closed-looped flight simulator 5 min after ten pairings of the US and CS. Conditioned bees were preferentially oriented to the CS after such training. This increase in preference for CS was maintained for 24 h, indicating the presence of long-term memory. Because the total orienting time was not altered by conditioning, conditioning did not enhance orientation activity itself but increased the relative time for orientation to CS. When 0.4 or 4 mM epinastine (an antagonist of octopamine receptors) was injected into the bee’s head 30 min prior to the experiment, both short- and long-term memory formation were significantly impaired, suggesting that octopamine, which is crucial for appetitive olfactory learning in insects, is also involved in visual orientation learning.

  Corresponding, Springer Science and Business Media LLC, Mar. 2023, Journal of Comparative Physiology A

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Possible Role of Polarized Light Information in Spatial Recognition in the Cricket Gryllus bimaculatus.

  Nobuaki Matsubara, Ryuichi Okada, Midori Sakura

  Many insects are able to use skylight e-vector patterns to deduce their heading direction. Crickets have been well known to orient themselves to certain e-vector orientations to keep their walking direction. However, it is still unknown if crickets are able to utilize polarized light information for spatial recognition. Using an experimental paradigm similar to the Morris water maze for rodents, here we examine the possibility that the cricket Gryllus bimaculatus can utilize polarized light information to find the target place. Crickets were placed in a round arena with a heated floor, a portion of which was cooled, and a cross-shaped e-vector pattern was presented from the top of the arena so that the cricket could find the cool spot by walking along the e-vector direction. When the arrangement of the e-vector pattern and the cool spot were fixed throughout the experiments, the time and the walking distance to find the cool spot were significantly decreased with increasing trials, but not when the e-vector pattern was rotated between each trial. Moreover, a model selection indicated that the visual stimulus contributed to the decrease in time and distance. To investigate the cricket's exploration patterns in the arena, a test trial in which the whole floor was uniformly heated was performed before and after the training trials. In the test trial, the crickets trained with the positionally fixed e-vector pattern showed wall-following behavior for a significantly longer time than those untrained and those trained with random e-vector patterns.

  Aug. 2021, Zoological science, 38(4) (4), 297 - 304, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Relationship between the flight distance of ambrosia beetle, Platypus quercivorus and environmental factors

  Fujihara Seima, Ito Yasuto, Pham Duy Long, Okada Ryuichi, Yamasaki Michimasa, Ikeno Hidetoshi

  [in Japanese]

  THE JAPANESE FORESTRY SOCIETY, May 2021, The Japanese Forest Society Congress, 132, 140, Japanese


• Differences between queen piping temporal structures of two honeybee species, Apis cerana and Apis mellifera

  Tetsu Yamamoto, Michio Sugahara, Ryuichi Okada, Hidetoshi Ikeno

  Apr. 2021, APIDOLOGIE, 52(2) (2), 524 - 534, English

  [Refereed]

  Scientific journal


• Females invest more energy in flight: flight characteristics of Platypus quercivorus (Murayama) revealed by a flight mill

  Duy Long Pham, Yasuto Ito, Ryuichi Okada, Hidetoshi Ikeno, Michimasa Yamasaki

  2021, Journal of Forest Research, 26(2) (2), 143 - 151

  [Refereed]

  Scientific journal


• Orientation to polarized light in tethered flying honeybees.

  Norihiro Kobayashi, Ryuichi Okada, Midori Sakura

  Many insects exploit the partial plane polarization of skylight for visual compass orientation and/or navigation. In the present study, using a tethering system, we investigated how flying bees respond to polarized light stimuli. The behavioral responses of honeybees (Apis mellifera) to a zenithal polarized light stimulus were observed using a tethered animal in a flight simulator. Flight direction of the bee was recorded by monitoring the horizontal movement of its abdomen, which was strongly anti-correlated with its torque. When the e-vector orientation of the polarized light was rotated clockwise or counterclockwise, the bee responded with periodic right-and-left abdominal movements; however, the bee did not show any clear periodic movement under the static e-vector or depolarized stimulus. The steering frequency of the bee was well coordinated with the e-vector rotation frequency of the stimulus, indicating that the flying bee oriented itself to a certain e-vector orientation, i.e. exhibited polarotaxis. The percentage of bees exhibiting clear polarotaxis was much smaller under the fast stimulus (3.6 deg s-1) compared with that under a slow stimulus (0.9 or 1.8 deg s-1). Bees did not demonstrate any polarotactic behavior after the dorsal rim area of the eyes, which mediates insect polarization vision in general, was bilaterally covered with black paint. Preferred e-vector orientations under the clockwise stimulus varied among individuals and distributed throughout -90 to 90 deg. Some bees showed similar preferred e-vector orientations for clockwise and counterclockwise stimuli whereas others did not. Our results strongly suggest that flying honeybees utilize the e-vector information from the skylight to deduce their heading orientation for navigation.

  Dec. 2020, The Journal of experimental biology, 223(Pt 23) (Pt 23), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Platypus quercivorus ambrosia beetles use leaf volatiles in host selection

  Duy Long Pham, Yasuto Ito, Ryuichi Okada, Hidetoshi Ikeno, Haruna Kazama, Naoki Mori, Michimasa Yamasaki

  Dec. 2020, Entomologia Experimentalis et Applicata, 168(12) (12), 928 - 939

  [Refereed]

  Scientific journal


• Effects of leaf conditions and flight activity on the behaviour of Platypus quercivorus (Murayama) (Coleoptera: Platypodidae)

  Duy Long Pham, Yasuto Ito, Ryuichi Okada, Hidetoshi Ikeno, Yuji Isagi, Michimasa Yamasaki

  Nov. 2019, Journal of Applied Entomology, 143(9) (9), 1000 - 1010

  [Refereed]

  Scientific journal


• Neuroethology of the Waggle Dance: How Followers Interact with the Waggle Dancer and Detect Spatial Information.

  Hiroyuki Ai, Ryuichi Okada, Midori Sakura, Thomas Wachtler, Hidetoshi Ikeno

  Since the honeybee possesses eusociality, advanced learning, memory ability, and information sharing through the use of various pheromones and sophisticated symbol communication (i.e., the "waggle dance"), this remarkable social animal has been one of the model symbolic animals for biological studies, animal ecology, ethology, and neuroethology. Karl von Frisch discovered the meanings of the waggle dance and called the communication a "dance language." Subsequent to this discovery, it has been extensively studied how effectively recruits translate the code in the dance to reach the advertised destination and how the waggle dance information conflicts with the information based on their own foraging experience. The dance followers, mostly foragers, detect and interact with the waggle dancer, and are finally recruited to the food source. In this review, we summarize the current state of knowledge on the neural processing underlying this fascinating behavior.

  Oct. 2019, Insects, 10(10) (10), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Heuristic Trajectory Decision Method to Enhance the Tracking Performance of Multiple Honeybees on a Flat Laboratory Arena

  Kimura Toshifumi, Ohashi Mizue, Crailsheim Karl, Schmickl Thomas, Okada Ryuichi, Radspieler Gerald, Isokawa Teijiro, Ikeno Hidetoshi

  In recent ethological studies, the behaviors and interactions of animals have been recorded by digital video cameras and webcams, which provide high functionality at reasonable cost. However, extracting the behavioral data from these videos is a laborious and time-consuming manual task. We recently proposed a novel method for tracking unmarked multiple honeybees in a flat arena, and developed a prototype software named "K-Track". The K-Track algorithm successfully resolved nearly 90% of cases involving overlapped or interacted insects, but failed when such events happened near an edge of a circular arena, which is commonly employed in experiments. In the present study, we improved our K-Track algorithm by comparing the interaction trajectories obtained from forward and backward playing of video episodes. If the tracking results differed between the forward and backward episodes, the trajectory with lower maximum moving distance per frame is chosen. Based on this concept, we developed a new software, "K-Track-kai", and compared the performances of K-Track and K-Track-kai in honeybee tracking experiments. In the cases of 6 and 16 honeybees, K-Track-kai improved the tracking accuracy from 91.7% to 96.4% and from 94.4% to 96.7%, respectively.

  THE INSTITUTE OF SYSTEMS, CONTROL AND INFORMATION ENGINEERS (ISCIE), 2019, Transactions of the Institute of Systems, Control and Information Engineers, 32(3) (3), 113 - 122, English

  [Refereed]


• Automatic tracking method for multiple honeybees using backward-play movies

  Toshifumi Kimura, Mizue Ohashi, Ryuichi Okada, Karl Crailsheim, Thomas Schmickl, Gerald Radspieler, Teijiro Isokawa, Hidetoshi Ikeno

  Apr. 2018, 2017 6th International Conference on Informatics, Electronics and Vision and 2017 7th International Symposium in Computational Medical and Health Technology, ICIEV-ISCMHT 2017, 2018-January, 1 - 4

  [Refereed]

  International conference proceedings


• Measuring the flight ability of the ambrosia beetle, Platypus quercivorus (Murayama), using a low-cost, small, and easily constructed flight mill

  OKADA Ryuichi, PHAM Duy Long, ITO Yasuto, YAMASAKI Michimasa, IKENO Hidetoshi

  The ambrosia beetle, Platypus quercivorus (Murayama), is the vector of a fungal pathogen that causes mass mortality of Fagaceae trees (Japanese oak wilt). Therefore, knowing the dispersal capacity may help inform trapping/tree removal efforts to prevent this disease more effectively. In this study, we measured the flight velocity and duration and estimated the flight distance of the beetle using a newly developed flight mill. The flight mill is low cost, small, and constructed using commonly available items. Both the flight mill arm and its vertical axis comprise a thin needle. A beetle specimen is glued to one tip of the arm using instant glue. The other tip is thick due to being covered with plastic, thus it facilitates the detection of rotations of the arm. The revolution of the arm is detected by a photo sensor mounted on an infrared LED, and is indicated by a change in the output voltage when the arm passed above the LED. The photo sensor is connected to a personal computer and the output voltage data are stored at a sampling rate of 1 kHz. By conducting experiments using this flight mill, we found that P. quercivorus can fly at least 27 km. Because our flight mill comprises cheap and small ordinary items, many flight mills can be prepared and used simultaneously in a small laboratory space. This enables experimenters to obtain a sufficient amount of data within a short period.

  2018, Journal of Visualized Experiments, 138(138) (138), e57468, English, International magazine

  [Refereed]

  Scientific journal


• Phototactic behavior of the ambrosia beetle Platypus quercivorus (Murayama) (Coleoptera: Platypodidae) before and after flight

  Duy Long Pham, Yasuto Ito, Ryuichi Okada, Hidetoshi Ikeno, Yuji Isagi, Michimasa Yamasaki

  May 2017, JOURNAL OF INSECT BEHAVIOR, 30(3) (3), 318 - 330, English

  [Refereed]

  Scientific journal


• Flight ablity of Platypus quercivorus measured by a flight mill

  Fukaya Satoshi, Okada Ryuichi, Itou Yasuto

  [in Japanese]

  THE JAPANESE FORESTRY SOCIETY, 2015, The Japanese Forest Society Congress, 126, 153, Japanese


• Error in the Honeybee Waggle Dance Improves Foraging Flexibility

  Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito

  Feb. 2014, SCIENTIFIC REPORTS, 4, 4175, English

  [Refereed]

  Scientific journal


• What are the elements of motivation for acquisition of conditioned taste aversion?

  Koichi Mita, Akiko Okuta, Ryuichi Okada, Dai Hatakeyama, Emi Otsuka, Miki Yamagishi, Mika Morikawa, Yuki Naganuma, Yutaka Fujito, Varvara Dyakonova, Ken Lukowiak, Etsuro Ito

  Jan. 2014, NEUROBIOLOGY OF LEARNING AND MEMORY, 107, 1 - 12, English

  [Refereed]

  Scientific journal


• Development of a New Method to Track Multiple Honey Bees with Complex Behaviors on a Flat Laboratory Arena

  Toshifumi Kimura, Mizue Ohashi, Karl Crailsheim, Thomas Schmickl, Ryuichi Okada, Gerald Radspieler, Hidetoshi Ikeno

  Jan. 2014, PLOS ONE, 9(1) (1), e84656, English

  [Refereed]

  Scientific journal


• Paired pulse ratio analysis of insulin-induced synaptic plasticity in the snail brain

  Jun Murakami, Ryuichi Okada, Yutaka Fujito, Manabu Sakakibara, Ken Lukowiak, Etsuro Ito

  May 2013, JOURNAL OF EXPERIMENTAL BIOLOGY, 216(10) (10), 1771 - 1773, English

  [Refereed]

  Scientific journal


• Involvement of Insulin-Like Peptide in Long-Term Synaptic Plasticity and Long-Term Memory of the Pond Snail Lymnaea stagnalis

  Jun Murakami, Ryuichi Okada, Hisayo Sadamoto, Suguru Kobayashi, Koichi Mita, Yuki Sakamoto, Miki Yamagishi, Dai Hatakeyama, Emi Otsuka, Akiko Okuta, Hiroshi Sunada, Satoshi Takigami, Manabu Sakakibara, Yutaka Fujito, Masahiko Awaji, Shunsuke Moriyama, Ken Lukowiak, Etsuro Ito

  Jan. 2013, JOURNAL OF NEUROSCIENCE, 33(1) (1), 371 - 383, English

  [Refereed]

  Scientific journal


• Increase in cyclic AMP concentration in a cerebral giant interneuron mimics part of a memory trace for conditioned taste aversion of the pond snail

  Emi Otsuka, Miho Matsunaga, Ryuichi Okada, Miki Yamagishi, Akiko Okuta, Ken Lukowiak, Etsuro Ito

  2013, Biophysics (Japan), 9, 161 - 166, English

  [Refereed]

  Scientific journal


• Detection of H2O2 by Fluorescence Correlation Spectroscopy

  Etsuro Ito, Satoshi Watabe, Mika Morikawa, Hiromi Kodama, Ryuichi Okada, Toshiaki Miura

  2013, HYDROGEN PEROXIDE AND CELL SIGNALING, PT A, 526, 135 - 143, English

  [Refereed]

  In book


• Memory Trace in Feeding Neural Circuitry Underlying Conditioned Taste Aversion in Lymnaea

  Etsuro Ito, Emi Otsuka, Noriyuki Hama, Hitoshi Aonuma, Ryuichi Okada, Dai Hatakeyama, Yutaka Fujito, Suguru Kobayashi

  Aug. 2012, PLOS ONE, 7(8) (8), e43151, English

  [Refereed]

  Scientific journal


• Waggle dance effect: dancing in autumn reduces the mass loss of a honeybee colony

  Ryuichi Okada, Tadaaki Akamatsu, Kanako Iwata, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito

  May 2012, JOURNAL OF EXPERIMENTAL BIOLOGY, 215(10) (10), 1633 - 1641, English

  [Refereed]

  Scientific journal


• Evidence for instantaneous e-vector detection in the honeybee using an associative learning paradigm

  Midori Sakura, Ryuichi Okada, Hitoshi Aonuma

  Lead, Feb. 2012, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 279(1728) (1728), 535 - 542, English

  [Refereed]

  Scientific journal


• 1D1534 Insulin and glucose for memory in a snail(Development, Differentiation, Neuroscience,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

  Ito Etsuro, Okada Ryuichi, Morikawa Mika, Takigami Satoshi, Okuta Akiko, Sakakibara Manabu

  The Biophysical Society of Japan General Incorporated Association, 2012, Seibutsu Butsuri, 52, S26, English


• Tracking of Multiple Honey Bees on a Flat Surface

  Toshifumi Kimura, Mizue Ohashi, Karl Crailsheim, Thomas Schmickl, Ryuichi Okada, Hidetoshi Ikeno

  2012, PROCEEDINGS OF THE 2012 FIFTH INTERNATIONAL CONFERENCE ON EMERGING TRENDS IN ENGINEERING AND TECHNOLOGY (ICETET 2012), 36 - 39, English

  [Refereed]

  International conference proceedings


• MATHEMATICAL ANALYSIS OF THE HONEYBEE WAGGLE DANCE

  R. Okada, H. Ikeno, T. Kimura, Mizue Ohashi, H. Aonuma, E. Ito

  2012, ACTA BIOLOGICA HUNGARICA, 63, 75 - 79, English

  [Refereed]

  Scientific journal


• INSULIN AND MEMORY IN LYMNAEA

  E. Ito, R. Okada, Yuki Sakamoto, Emi Otshuka, K. Mita, Akiko Okuta, H. Sunada, M. Sakakibara

  2012, ACTA BIOLOGICA HUNGARICA, 63, 194 - 201, English

  [Refereed]

  Scientific journal


• A new approach for the simultaneous tracking of multiple honeybees for analysis of hive behavior

  Toshifumi Kimura, Mizue Ohashi, Ryuichi Okada, Hidetoshi Ikeno

  Sep. 2011, APIDOLOGIE, 42(5) (5), 607 - 617, English

  [Refereed]

  Scientific journal


• Highly Sensitive Determination of Hydrogen Peroxide and Glucose by Fluorescence Correlation Spectroscopy

  Satoshi Watabe, Yuki Sakamoto, Mika Morikawa, Ryuichi Okada, Toshiaki Miura, Etsuro Ito

  Aug. 2011, PLOS ONE, 6(8) (8), e22955, English

  [Refereed]

  Scientific journal


• Markov Model of Honeybee Social Behavior

  Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito

  May 2010, INFORMATION-AN INTERNATIONAL INTERDISCIPLINARY JOURNAL, 13(3B) (3B), 1115 - 1130, English

  [Refereed]

  Scientific journal


• 3P257 Lon-term potentiation induced by insulin-like peptide in the central nervous system of a snail(Neuronal Circuit & Information processing,The 48th Annual Meeting of the Biophysical Society of Japan)

  Ito Etsuro, Murakami Jun, Okada Ryuichi, Okuta Akiko, Sadamoto Hisayo

  The Biophysical Society of Japan General Incorporated Association, 2010, Seibutsu Butsuri, 50(2) (2), S190, English


• Observation system for the control of the hive environment by the honeybee (Apis mellifera)

  Mizue Ohashi, Ryuichi Okada, Toshifumi Kimura, Hidetoshi Ikeno

  Aug. 2009, BEHAVIOR RESEARCH METHODS, 41(3) (3), 782 - 786, English

  [Refereed]

  Scientific journal


• Gamma-Aminobuyric Acid (GABA)-Mediated Neural Connections in the Drosophila Antennal Lobe

  Ryuichi Okada, Takeshi Awasaki, Kei Ito

  May 2009, JOURNAL OF COMPARATIVE NEUROLOGY, 514(1) (1), 74 - 91, English

  [Refereed]

  Scientific journal


• The tracking system of honeybee behavior in the observation hive

  Toshifumi Kimura, Hidetoshi Ikeno, Mizue Ohashi, Ryuichi Okada, Etsuro Ito

  Dec. 2008, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 151(4) (4), 455 - 456, English

  [Refereed]


• A movement index for evaluation of honeybee activity in the observation hive

  Hidetoshi Ikeno, Ryuichi Okada, Mizue Ohashi, Toshifumi Kimura, Tadaaki Akamatsu, Etsuro Ito

  Dec. 2008, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 151(4) (4), 456 - 456, English

  [Refereed]


• Locomotion patterns of honeybee in the hive

  Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma, Etsuro Ito

  Dec. 2008, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 151(4) (4), 456 - 456, English


• Olfactory Information Processing in the Drosophila Antennal Lobe: Anything Goes?

  Ana F. Silbering, Ryuichi Okada, Kei Ito, C. Giovanni Galizia

  Dec. 2008, JOURNAL OF NEUROSCIENCE, 28(49) (49), 13075 - 13087, English

  [Refereed]

  Scientific journal


• Drosophila olfactory local interneurons and projection neurons derive from a common neuroblast lineage specified by the empty spiracles gene

  Abhijit Das, Sonia Sen, Robert Lichtneckert, Ryuichi Okada, Kei Ito, Veronica Rodrigues, Heinrich Reichert

  Dec. 2008, NEURAL DEVELOPMENT, 3, 33, English

  [Refereed]

  Scientific journal


• The dance of the honeybee: How do honeybees dance to transfer food information effectively?

  R. Okada, H. Ikeno, Noriko Sasayama, H. Aonuma, D. Kurabayashi, E. Ito

  2008, ACTA BIOLOGICA HUNGARICA, 59, 157 - 162, English

  [Refereed]

  Scientific journal


• Speed of back-swimming of Lymnaea

  Kanako Aono, A. Fusada, Y. Fusada, W. Ishii, Y. Kanaya, Mami Komuro, Kanae Matsui, S. Meguro, Ayumi Miyamae, Yurie Miyamae, Aya Murata, Shizuka Narita, Hiroe Nozaka, Wakana Saito, Ayumi Watanabe, Kaori Nishikata, A. Kanazawa, Y. Fujito, R. Okada, K. Lukowiak, E. Ito

  2008, ACTA BIOLOGICA HUNGARICA, 59, 105 - 109, English

  [Refereed]

  Scientific journal


• Biological insights into robotics: honeybee foraging behavior by waggle dance

  OKADA Ryuichi, IKENO Hidetoshi, AONUMA Hitoshi, ITO Etsuro

  2008, Advanced Robotics, 22, 1665 - 1681, English

  [Refereed]

  Scientific journal


• Activity-dependent plasticity in an olfactory circuit

  Silke Sachse, Erroll Rueckert, Andreas Keller, Ryuichi Okada, Nobuaki K. Tanaka, Kei Ito, Leslie B. Vosshall

  Dec. 2007, NEURON, 56(5) (5), 838 - 850, English

  [Refereed]

  Scientific journal


• Learning-related plasticity in PE1 and other mushroom body-extrinsic neurons in the honeybee brain

  Ryuichi Okada, Jurgen Rybak, Gisela Manz, Randolf Menzel

  Oct. 2007, JOURNAL OF NEUROSCIENCE, 27(43) (43), 11736 - 11747, English

  [Refereed]

  Scientific journal


• Cautionary observations on preparing and interpreting brain images using molecular biology-based staining techniques

  K Ito, R Okada, NK Tanaka, T Awasaki

  Oct. 2003, MICROSCOPY RESEARCH AND TECHNIQUE, 62(2) (2), 170 - 186, English

  [Refereed]

  Scientific journal


• Distribution of dendrites of descending neurons and its implications for the basic organization of the cockroach brain

  R Okada, M Sakura, M Mizunami

  Lead, Mar. 2003, JOURNAL OF COMPARATIVE NEUROLOGY, 458(2) (2), 158 - 174, English

  [Refereed]

  Scientific journal


• Olfactory discrimination of structurally similar alcohols by cockroaches

  M Sakura, R Okada, M Mizunami

  Nov. 2002, JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 188(10) (10), 787 - 797, English

  [Refereed]

  Scientific journal


• Sensory responses and movement-related activities in extrinsic neurons of the cockroach mushroom bodies

  R Okada, J Ikeda, M Mizunami

  Lead, Aug. 1999, JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 185(2) (2), 115 - 129, English

  [Refereed]

  Scientific journal


• Mushroom bodies of the cockroach: Activity and identities of neurons recorded in freely moving animals

  M Mizunami, R Okada, YS Li, NJ Strausfeld

  Dec. 1998, JOURNAL OF COMPARATIVE NEUROLOGY, 402(4) (4), 501 - 519, English

  [Refereed]

  Scientific journal


• Topography of modular subunits in the mushroom bodies of the cockroach

  M Mizunami, M Iwasaki, R Okada, M Nishikawa

  Sep. 1998, JOURNAL OF COMPARATIVE NEUROLOGY, 399(2) (2), 153 - 161, English

  [Refereed]

  Scientific journal


• Topography of four classes of Kenyon cells in the mushroom bodies of the cockroach

  M Mizunami, M Iwasaki, R Okada, M Nishikawa

  Sep. 1998, JOURNAL OF COMPARATIVE NEUROLOGY, 399(2) (2), 162 - 175, English

  [Refereed]

  Scientific journal


• Modular structures in the mushroom body of the cockroach

  M Mizunami, M Iwasaki, M Nishikawa, R Okada

  Jul. 1997, NEUROSCIENCE LETTERS, 229(3) (3), 153 - 156, English

  [Refereed]

  Scientific journal

• 飛翔時間がカシナガの姿勢に及ぼす影響~フライトミルを用いた観察~

  小西温輝, 大橋瑞江, 池野英利, 岡田龍一, 山崎理正, 伊東康人

  2024, 日本森林学会大会学術講演集, 135th


• カシノナガキクイムシの宿主選択に関わる揮発性物質に対する触角の応答

  岡田龍一, 伊東康人, 山崎理正

  2024, 日本応用動物昆虫学会大会講演要旨, 68th


• カシノナガキクイムシにおける嗅覚1次中枢の構造と集合フェロモンの情報処理機構

  岡田龍一, 山崎理正, 伊東康人

  2021, 日本応用動物昆虫学会大会講演要旨, 65th


• Causes of high variation in flight distance of the ambrosia beetle Platypus quercivorus

  山崎理正, PHAM Duy Long, 伊東康人, 岡田龍一, 池野英利

  2021, 日本生態学会大会講演要旨(Web), 68th


• カシノナガキクイムシの嗅覚1次中枢の構造と集合フェロモンに対する応答

  岡田龍一, 山崎理正, 伊東康人

  2020, 日本応用動物昆虫学会大会講演要旨, 64th


• フライトミルによるカシノナガキクイムシ飛翔行動の活性化要因の解明

  藤原聖真, 山崎理正, 岡田龍一, PHAM Duy Long, 伊東康人, 池野英利

  2020, 日本森林学会大会学術講演集, 131st


• Flight behaviors of the ambrosia beetle Platypus quercivorus revealed by a flight mill

  PHAM DUY Long, ITO Yasuo, OKADA Ryuichi, IKENO Hidetoshi, YAMASAKI Michimasa

  Mar. 2019, 日本森林学会大会学術講演集, 130th, 167, English

  Summary national conference


• 集合フェロモン濃度変化がカシノナガキクイムシ飛翔特性に及ぼす影響

  石野貴大, 岡田龍一, 伊東康人, PHAM Long Duy, 山崎理正, 池野英利

  26 Mar. 2018, 日本森林学会大会学術講演集, 129th, 177, Japanese


• Behavioral responses of the ambrosia beetle Platypus quercivorus to chemical volatiles from host leaves

  PHAM DUY Long, YAMASAKI Michimasa, ITO Yasuto, OKADA Ryuichi, IKENO Hidetoshi

  Mar. 2018, 日本森林学会大会学術講演集, 129th, 176, English

  Summary national conference


• ミツバチの天空コンパスナビゲーション-空を見て方向を知るしくみ

  SAKURA MIDORI, 岡田 龍一

  Jan. 2018, 生物の科学 遺伝, 72(1) (1), 2 - 6, Japanese

  [Refereed]

  Introduction commerce magazine


• ミツバチの天空コンパスナビゲーション ―空を見て方向を知るしくみ

  SAKURA Midori, Okada Ryuichi

  Jan. 2018, 生物の科学 遺伝, 72, 2 - 6, Japanese

  Introduction scientific journal


• 集合フェロモンによってカシノナガキクイムシの飛翔特性に違いが生じるか?

  奥田直人, 岡田龍一, PHAM Long Duy, 伊東康人, 山崎理正, 池野英利

  Mar. 2017, 日本森林学会大会学術講演集, 128th, 133, Japanese

  Summary national conference


• ミツバチコロニーのエネルギー収支の見積もり-成長期と退行期の比較

  吉田澪, 大橋瑞江, 木村敏文, 岡田龍一, 池野英利

  2017, 日本生態学会大会講演要旨(Web), 64th


• 昆虫の脳と匂いの記憶

  岡田 龍一

  2017, アグリバイオ, 1, 76 - 81, Japanese

  Introduction commerce magazine


• What is ability for flight of the Platypus quercivorus decided by

  Fukaya Satoshi, Okuda Naoto, Okada Ryuiti, Ito Yasuto, Ikeno Hidetoshi, Yamasaki Mitimasa

  [in Japanese]

  THE JAPANESE FORESTRY SOCIETY, Mar. 2016, The Japanese Forest Society Congress, 127th, 225 - 151, Japanese

  Summary national conference


• ミツバチの採餌行動におけるエネルギー収支

  澤井穂高, 岡田龍一, 吉田澪, 大橋瑞江, 木村敏文, 池野英利

  2016, 日本生態学会大会講演要旨(Web), 63th


• ミツバチコロニーにおける採餌活動の日的変化および季節的変化の解析

  吉田澪, 大橋瑞江, 木村敏文, 岡田龍一, 池野英利

  2015, 日本生態学会大会講演要旨(Web), 62nd


• 空間を考慮したミツバチの巣板モデルとシミュレーション

  中桐斉之, 大橋瑞江, 岡田龍一, 池野英利

  2015, 日本生態学会大会講演要旨(Web), 62nd


• 昆虫の脳と匂いの記憶

  岡田 龍一

  ニューサイエンス社, 2015, 昆虫と自然, 50(14) (14), 40 - 44, Japanese

  Introduction commerce magazine


• ミツバチコロニーの生存維時における8の字ダンスの効果

  岡田龍一, 栄美紗, 木村敏文, 大橋瑞江, 池野英利

  2014, 日本動物学会大会予稿集, 85th


• 「脳科学のフロンティア」 脳はどこまでわかってきたか：神経可塑性に着目して

  岡田 龍一, 伊藤 悦朗

  丸善, 2013, パリティ, 28(4) (4), 24 - 32, Japanese

  [Refereed]

  Introduction commerce magazine


• Involvement of nitric oxide in memory formation in microbrains

  ITO Etsuro, MATSUO Ryota, OKADA Ryuichi

  2013, Neuroscience Letters, 541, 1 - 3, English

  [Refereed]

  Introduction scientific journal


• 動的環境下でのミツバチ採餌行動におけるダンス情報の誤差の効果

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  20 Aug. 2012, 日本動物学会大会予稿集, 83rd, 128, Japanese


• モノアラガイの長期記憶におけるインスリンとグルコースの役割

  伊藤悦朗, 箕田康一, 岡田龍一, 森川美佳, 山岸美貴, 大塚栄美

  2012, 日本動物学会中国四国支部会報, (64) (64), 20, Japanese


• ミツバチの尻振りダンスと採餌行動における効果

  岡田 龍一

  2012, 比較生理生化学, 29, 121 - 130, Japanese

  [Refereed]

  Introduction scientific journal


• コンピュータシミュレーションによるミツバチ8の字ダンスの採餌行動における効果

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  20 Aug. 2011, 日本動物学会大会予稿集, 82nd, 145, Japanese


• コンピュータシミュレーションによるミツバチの採餌戦略の解析

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  2011, 日本動物学会中国四国支部会報, (63) (63), 16, Japanese


• 計算機シミュレーションによるミツバチ8の字ダンスの採餌行動における効果

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  20 Aug. 2010, 日本動物学会大会予稿集, 81st, 155, Japanese


• 数理モデルによるミツバチの8の字ダンスの生物的意義の解析

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  2010, 日本比較生理生化学会大会予稿集, 32nd, 29, Japanese


• ミツバチの採餌行動は尻振りダンス行動によって効率的になるのか?

  岡田龍一, 池野英利, 赤松忠明, 岩田可南子, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  2010, 日本動物学会中国四国支部会報, (62) (62), 12, Japanese


• Effects of a waggle dance on the honeybee foraging behavior

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  2009, 日本動物学会大会予稿集, 80th (Web)


• Tracking system of plural honeybees for behavioral analysis

  木村敏文, 池野英利, 大橋瑞江, 岡田龍一

  2009, 日本動物学会大会予稿集, 80th (Web)


• Analysis of carbon flow using continuous measuring system in honeybee colony

  岩田可南子, 池野英利, 大橋瑞江, 木村敏文, 岡田龍一, 赤松忠明

  2009, 日本動物学会大会予稿集, 80th (Web)


• ミツバチの尻振りダンスとコロニーの集蜜量の関係

  岡田龍一, 赤松忠明, 岩田可南子, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  2009, 日本比較生理生化学会大会予稿集, 31st, 69, Japanese


• セイヨウミツバチの偏光知覚機構の解明

  佐倉緑, 岡田龍一, 青沼仁志

  2009, 日本比較生理生化学会大会予稿集, 31st, 70, Japanese


• ショウジョウバエ脳のGABAニューロンの局在と役割

  岡田 龍一, 伊藤 啓

  2009, 生体の科学, 60, 394 - 395, Japanese

  Introduction scientific journal


• 巣内での追従バチの行動パターン

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  20 Aug. 2008, 日本動物学会大会予稿集, 79th, 132, Japanese


• A basic study of tracking honeybees in the observation hive using vector quantization method

  KIMURA Toshifumi, IKENO Hidetoshi, OHASHI Mizue, OKADA Ryuichi, ITO Etsuro

  The analysis of biological function produced by both of individual and assemblage activity of animals is a challenging topic of behavioral ecology. Social insects are possible tool for the analysis because numerous studies have been already done to clarify the behavioral factors of individuals. Especially, honeybee is an ideal animal for the experiments because of the development of breeding techniques, existence of clear caste according to their age, efficient forage behavior adaptive for variable feeding places and high ability of honey storage in their colony. Seeley et al. conducted many behavioral experiments in order to scrutinize honeybee behavior inside and outside of the nests. However, these experiments normally have to put numbers on each body of thousands of honeybees to identify individuals and follow their behavior by hand, which require huge time and labors. In this study, we developed a system to identify honeybee in a nest image and to follow plural honeybees simultaneously. The system is composed by three parts, determination of the region of honeybee body from the other parts with vector quantization, extraction of the region of single honeybee bodies, and reorganization of single honeybees. Our system succeeded to identify nearly 70% of honeybees in a hive automatically.

  The Institute of Electronics, Information and Communication Engineers, 06 Mar. 2008, IEICE technical report, 107(541) (541), 113 - 116, Japanese


• 巣内ミツバチ活動を評価するための時空間運動指標

  池野英利, 岡田龍一, 大橋瑞江, 木村敏文, 赤松忠明, 伊藤悦朗

  2008, 日本比較生理生化学会大会予稿集, 30th


• ミツバチの観察巣板内行動追跡システム

  木村敏文, 池野英利, 大橋瑞江, 岡田龍一, 伊藤悦朗

  2008, 日本比較生理生化学会大会予稿集, 30th


• 巣内ミツバチの歩行パターン

  岡田龍一, 池野英利, 木村敏文, 大橋瑞江, 青沼仁志, 伊藤悦朗

  2008, 日本比較生理生化学会大会予稿集, 30th, 29, Japanese


• 社会的適応行動から学ぶ情報共有システムの構築―ミツバチの８の字ダンスを対象として

  岡田 龍一, 池野 英利, 青沼 仁志, 倉林 大輔, 伊藤 悦朗

  2007, 計測と制御, 46, 916 - 921, Japanese

  [Refereed]

  Introduction scientific journal


• The basic design of the insect brain for motor control

  Ryuichi Okada

  Nov. 2006, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 145(3-4) (3-4), 401 - 401, English

  Summary international conference


• Distribution of dendrites of descending neurons and its implications for the basic organization of the cockroach brain. (vol 458, pg 158, 2003)

  R Okada, M Sakura, M Mizunami

  May 2003, JOURNAL OF COMPARATIVE NEUROLOGY, 459(3) (3), 327 - +, English

  Others


• 昆虫の嗅覚学習

  水波 誠, 松本 幸久, 佐倉 緑, 岡田 龍一

  北海道大学, 1999, 電子科学研究, 7, 69 - 71, Japanese

• 研究者が教える動物実験 第３巻：行動

  OKADA RYUICHI, SAKURA MIDORI, AI HIROYUKI

  Joint work, 共立出版, 2015, Japanese

  General book


• 研究者が教える動物実験 第２巻：神経・筋

  OKADA RYUICHI

  Joint work, 共立出版, 2015, Japanese

  General book


• 研究者が教える動物実験 第１巻：感覚

  OKADA RYUICHI

  Joint work, 共立出版, 2015, Japanese

  General book


• Memory Consolidation

  OKADA RYUICHI

  Joint work, NOVA Science Publisher, 2015, English

  Scholarly book


• ミツバチのダンス ダンス 「行動生物学辞典」

  OKADA RYUICHI

  Joint work, 東京化学同人, 2013, Japanese

  Dictionary or encycropedia


• 分子昆虫学 −ポストゲノムの昆虫研究−

  OKADA RYUICHI

  Joint work, 共立出版, 2009, Japanese

  Scholarly book


• 身近な動物を使った実験４ ミツバチ コオロギ スズメガ

  OKADA RYUICHI

  Joint work, 三共出版, 2009, Japanese

  General book

• Life history of navigational exploration and social communication in honeybees

  Chen X, Okada R, Walter S, Sakura M, Xing Y, Menzel R

  13th Göttingen Meeting of the German Neuroscience Society, Mar. 2019, English, Göttingen, Germany, International conference

  Poster presentation


• Place memory based on visual information in the cricket Gryllus bimaculatus

  Matsubara N, Okada R, Sakura M

  日本比較生理学会第40回神戸大会, Nov. 2018, English, 神戸, Domestic conference

  Poster presentation


• ミツバチの嗅覚－味覚連合学習におけるオクトパミンの役割

  小林 樹里, 岡田 龍一, 佐倉 緑

  日本動物学会第89回札幌大会, Sep. 2018, Japanese, 札幌, Domestic conference

  Poster presentation


• フタホシコオロギの視覚情報に基づく場所記憶形成

  松原 伸明, 岡田 龍一, 佐倉 緑

  日本動物学会第89回札幌大会, Sep. 2018, Japanese, 札幌, Domestic conference

  Poster presentation


• Honeybees choose their way to home using e-vector information from the sky

  Sakura M, Onishi H, Okuyama A, Matoba N, Kobayashi N, Okada R

  International Congress for Neuroethology 2018, Jul. 2018, English, Brisbane, Australia, International conference

  Poster presentation


• 集合フェロモン濃度変化がカシノナガキクイムシ飛翔特性に及ぼす影響

  石野 貴大, 岡田 龍一, 伊東 康人, Long Duy Pham, 山崎 理正, 池野 英利

  日本森林学会, 2018, Japanese, Domestic conference

  Oral presentation


• ダンス追従バチの巣内でのふるまい、ミツバチシンポジウム

  岡田 龍一

  ミツバチシンポジウム, 2018, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Behavioral responses of the ambrosia beetle Platypus quercivorus to chemical volatiles from host leaves

  PHAM Duy Long, YAMASAKI Masamichi, ITO Yasuto, OKADA Ryuichi, IKENO Hidetoshi

  日本森林学会, 2018, English, Domestic conference

  Oral presentation


• Place memory based on visual information in the cricket Gryllus bimaculatus

  MATSUBARA Nobuaki, OKADA Ryuichi, SAKURA Midori

  日本比較生理生化学会第39回福岡大会, Nov. 2017, English, 福岡大学, Domestic conference

  Poster presentation


• 数理モデルを用いたミツバチコロニーサイズの成長と縮小特性の再現

  甘中 健一, 岡田 龍一, 池野 英利, 木村 敏文, 大橋 瑞江

  日本生態学会, 2017, Japanese, Domestic conference

  Oral presentation


• 集合フェロモンによってカシノナガキクイムシの飛翔特性に違いが生じるか？

  奥田 直人, 岡田 龍一, PHAM Duy Long, 伊東 康人, 山崎 理正, 池野 英利

  日本森林学会, 2017, Japanese, Domestic conference

  Oral presentation


• ミツバチは天空のeベクトル情報に基づいて飛行方向を決定する

  SAKURA MIDORI, 奥山 晶子, 的場 なつみ, 小林 宜弘, 岡田 龍一

  日本動物学会, 2017, Japanese, Domestic conference

  Oral presentation


• ミツバチの尻振りダンスにかくされた餌集めの工夫

  岡田 龍一

  ミツバチ科学研究会, 2017, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ミツバチコロニーのエネルギー収支の見積もり-成長期と退行期の比較

  吉田 澪, 大橋 瑞江, 木村 敏文, 岡田 龍一, 池野 英利

  日本生態学会, 2017, Japanese, Domestic conference

  Oral presentation


• ニホンミツバチにおける女王パイピング（トゥーティング、クワッキング）の特徴および分蜂との関係

  山本 哲, 菅原 道夫, 池野 英利, 岡田 龍一

  日本動物学会, 2017, Japanese, Domestic conference

  Oral presentation


• ダンス追従バチは複数のダンス蜂を追従する

  岡田 龍一, 池野 英利, 青沼 仁志

  日本動物学会, 2017, Japanese, Domestic conference

  Oral presentation


• Place memory based on visual information in the cricket Gryllus bimaculatus

  MATSUBARA Nobuaki, OKADA Ryuichi, SAKURA Midori

  日本比較生理学会, 2017, Japanese, Domestic conference

  Poster presentation


• Behavior of follower bees in a hive before departure after the dance communication

  OKADA Ryuichi, IKENO Hidetoshi, AONUMA Hitoshi

  日本比較生理学会, 2017, Japanese, Domestic conference

  Poster presentation


• ミツバチの採餌行動におけるエネルギー収支

  澤井 穂高, 岡田 龍一, 吉田 澪, 大橋 瑞江, 木村 敏文, 池野 英利

  日本生態学会, 2016, Japanese, Domestic conference

  Poster presentation


• ミツバチのコミュニケーション：個体の振るまい、コロニーの振るまい

  岡田 龍一

  ミツバチシンポジウム, 2016, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• カシノナガキクイムシの飛翔能力は何によって決まるのか

  深谷 智史, 奥田 直人, 岡田 龍一, 伊東 康人, 池野 英利, 山崎 理正

  日本森林学会, 2016, Japanese, Domestic conference

  Oral presentation


• Seasonal changes of energy gain and consumption in a honeybee colony

  YOSHIDA Mio, OHASHI Mizue, KIMURA Toshifumi, OKADA Ryuichi, IKENO Hidetoshi

  日本生態学会, 2016, English, Domestic conference

  Oral presentation


• Phototaxis behavior of ambrosia beetl Platypus quercivorus before and after flight

  PHAM Duy Long, YAMASAKI Masamichi, ITO Yasuto, OKADA Ryuichi, IKENO Hidetoshi

  日本生態学会, 2016, English, Domestic conference

  Oral presentation


• Optimal selection of honeybee waggle dance types for colony survival

  OKADA Ryuichi, IKENO Hidetoshi, SAKAE Misa, IIZUKA S, KIMURA Toshifumi, OHASHI Mizue

  日本比較生理学会, 2016, Japanese, Domestic conference

  Poster presentation


• Improvement in processing time of the tracking software, K-Track

  KIMURA Toshifumi, OHASHI Mizue, OKADA Ryuichi, IKENO Hidetoshi

  日本比較生理学会, 2016, Japanese, Domestic conference

  Poster presentation


• Honeybees choose their way to home using e-vector information from the sky

  SAKURA Midori, OKUYAMA Akiko, MATOBA Natsumi, KOBAYASHI Norihiro, OKADA Ryuichi

  日本比較生理学会, 2016, Japanese, Domestic conference

  Poster presentation


• Behavioral pattern of a follower bee in the dance communication

  OKADA Ryuichi, AONUMA Hitoshi, IKENO Hidetoshi

  The 22nd International Congress of Zoology, 2016, English, International conference

  Poster presentation


• ミツバチコロニーにおける採餌活動の日的変化および季節的変化の解析

  吉田 澪, 大橋 瑞江, 木村 敏文, 岡田 龍一, 池野 英利

  日本生態学会, 2015, Japanese, Domestic conference

  Oral presentation


• フライトミルを用いたカシノナガキクイムシの飛翔能力の測定

  深谷 智史, 岡田 龍一, 伊東 康人, 池野 英利

  日本森林学会, 2015, Japanese, Domestic conference

  Oral presentation


• ニホンミツバチにおける音コミュニケーション

  山本 哲, 菅原 道夫, 岡田 龍一, 池野 英利

  日本動物学会, 2015, Japanese, Domestic conference

  Oral presentation


• セイヨウミツバチの採餌経験に基づく偏光定位行動の解析

  SAKURA MIDORI, 的場 なつみ, 小林 宜弘, 岡田 龍一

  日本動物学会, 2015, Japanese, Domestic conference

  Oral presentation


• コンピュータシミュレーションによるミツバチのコロニーの生存戦略の解析

  岡田 龍一, 栄 美紗, 木村 敏文, 大橋 瑞江, 池野 英利

  日本動物学会, 2015, Japanese, Domestic conference

  Oral presentation


• Detection of bees’ trajectories in the backward movie using a tracking software, K-Track

  KIMURA Toshifumi, OHASHI Mizue, CRAILSHEIM Karl, SCHMICKL Thomas, OKADA Ryuichi, IKENO Hidetoshi

  Comp Biol 2015 （日本比較生理学会・日本内分泌学会合同大会, 2015, Japanese, Domestic conference

  Poster presentation


• Behavioral pattern of a follower bee during the honeybee dance communication

  OKADA Ryuichi, IKENO Hidetoshi, KIMURA Toshifumi, OHASHI Mizue, AONUMA Hitoshi, ITO Etsuro

  Comp Biol 2015 （日本比較生理学会・日本内分泌学会合同大会, 2015, Japanese, Domestic conference

  Poster presentation


• ミツバチコロニーの生存維持における８の字ダンスの効果

  岡田 龍一, 栄 美紗, 木村 敏文, 大橋 瑞江, 池野 英利

  日本動物学会, 2014, Japanese, Domestic conference

  Oral presentation


• マルチモーダル刺激における衝突回避および飛行安定行動の解析

  東條 美紀, 長谷川 雄二, 岡田 龍一, 池野 英利

  日本動物学会, 2014, Japanese, Domestic conference

  Oral presentation


• ニホンミツバチとセイヨウミツバチにおけるキンリョウヘンの匂いに対する感覚応答と学習効果

  原田 礼花, SAKURA MIDORI, 藍 浩之, 菅原 道夫, 岡田 龍一

  日本動物学会, 2014, Japanese, Domestic conference

  Oral presentation


• Spiking activity of an identified mushroom body extrinsic neuron during olfactory memory acquisition in the honeybee

  OKADA Ryuichi, MENZEL Randolf

  The 11th International Congress of Neuroethology, 2014, English, International conference

  Poster presentation


• Sensory responses to the oriental orchid odors in the Japanese and European honeybees

  HARADA Ayaka, AI Hiroyuki, SUGAHARA Michio, OKADA Ryuichi, SAKURA Midori

  The 11th International Congress of Neuroethology, 2014, English, International conference

  Poster presentation


• Orientation to the polarized light in flying honeybees

  SAKURA Midori, KOBAYASHI Norihiro, OKADA Ryuichi

  The 11th International Congress of Neuroethology, 2014, English, International conference

  Poster presentation


• Olfactory learning-related plasticity of the mushroom body neurons in the honeybee

  OKADA Ryuichi, MENZEL Randolf

  Hokkaido Neuroethology Workshops 2014, 2014, English, International conference

  [Invited]

  Invited oral presentation


• Improving tracking accuracy of the software to track multiple honey bees, K-Track

  KIMURA Toshifumi, OHASHI Mizue, CRAILSHEIM Karl, SCHMICKL Thomas, OKADA Ryuichi, RADSPIELER Gerald, IKENO Hidetoshi

  The 11th International Congress of Neuroethology, 2014, English, International conference

  Poster presentation


• 飛行中のミツバチの偏光定位行動

  SAKURA MIDORI, 小林 宜弘, 岡田 龍一

  日本動物学会, 2013, Japanese, Domestic conference

  Oral presentation


• 誤差情報を含んだ８の字ダンスによるミツバチの適応的採餌行動

  岡田 龍一

  無脊椎動物神経生物研究会, 2013, Japanese, Domestic conference

  Oral presentation


• 蛍光相関分光法を用いたグルコースの高感度測定

  伊藤 悦朗, 森川 美佳, 児玉 裕美, 岡田 龍一, 渡部 聡, 三浦 敏明

  日本薬学会, 2013, Japanese, Domestic conference

  Oral presentation


• ミツバチが匂いを記憶している時の脳の活動

  岡田 龍一

  ミツバチミニシンポジウム, 2013, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Orientation to the polarized light in flying honeybees

  SAKURA Midori, KOBAYASHI Norihiro, OKADA Ryuichi

  International Conference on Invertebrate Vision, 2013, English, International conference

  Poster presentation


• Development of a tracking program, K-Track, for analyzing honeybee’s behaviors

  KIMURA Toshifumi, OHASHI Mizue, CRAILSHEIM Karl, SCHMICKL Thomas, OKADA Ryuichi, RADSPIELER Gerald, IKENO Hidetoshi

  日本比較生理学会, 2013, Japanese, Domestic conference

  Poster presentation


• Consolidation of long-term memory by molluscan insulin-related peptide in Lymnaea

  ITO Etsuro, OKADA Ryuichi, HATAKEYAMA Dai, OTSUKA Emi, OKUTA Akiko

  日本比較生理学会, 2013, Japanese, Domestic conference

  Poster presentation


• ミツバチのダンスはどんな効果があるのか？

  岡田 龍一

  ミニシンポジウム：ミツバチの行動と社会性, 2012, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ダンス行動によるミツバチコロニーの環境適応：コンピュータシミュレーションによる解析

  岡田 龍一

  日本動物学会, 2012, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Neural network involved in olfactory learning in the honeybee brain

  OKADA Ryuichi

  日本神経科学会, 2012, English, Domestic conference

  [Invited]

  Invited oral presentation


• 数理モデルをもちいた８の字ダンスによるミツバチの適応的採餌行動の解析

  岡田 龍一

  自律分散システムシンポジウム, 2011, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ミツバチにおける８の字ダンスによる情報伝達とその効果

  岡田 龍一

  Animal2011（日本動物心理学会、日本動物行動学会、応用動物行動学会、日本家畜管理学会合同大会）, 2011, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ８の字ダンスによるミツバチコロニーの採餌戦略

  岡田 龍一

  日本進化学会, 2008, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Foraging strategy of the honeybee colony by waggle dance

  OKADA Ryuichi

  International Seminar on Current Issues in Social Insect Research -In Case of Honeybee and Ants, 2008, English, International conference

  [Invited]

  Invited oral presentation


• 昆虫のシンボリック言語

  岡田 龍一

  日本機械学会, 2007, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ミツバチの餌と匂いの連合学習によるキノコ体出力ニューロンの可塑性

  岡田 龍一

  ミツバチとショウジョウバエの比較分子神経生物学～脳と行動進化を遺伝子レベルで捉えなおす～, 2007, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 運動制御における昆虫の脳の基本設計

  岡田 龍一

  日本比較生理生化学会, 2006, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ミツバチのにおい学習と埋め込みワイヤ電極

  岡田 龍一

  日本動物学会, 2006, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ショウジョウバエ触角葉内のGABA介在性神経連絡

  岡田 龍一

  日本味と匂学会セミナー, 2005, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• GABA-mediated neural connections in the Drosophila antennal lobe

  OKADA Ryuichi

  日本比較生理生化学会, 2005, English, Domestic conference

  [Invited]

  Invited oral presentation


• 昆虫のキノコ体の慢性記録と運動制御における脳の基本設計

  岡田 龍一

  COE昆虫科学セミナー, 2004, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Implications for the basic organization for motor control in the cockroach brain

  OKADA Ryuichi

  Japanese-French Postdoc Meeting on Insect Neuroethology, 2004, English, International conference

  [Invited]

  Invited oral presentation

• 昆虫ナビゲーションにおける偏光コンパスの概日時計に基づく時間補償機構

  佐倉 緑, 藍 浩之, 岡田 龍一, 志垣 俊介, 渕側 太郎

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2022 - 31 Mar. 2027

  昆虫は天空の偏光e-ベクトルから自らの進行方向を検出する。その際、太陽の移動にともなうe-ベクトル方向の変化が概日時計によって補償されると考えられるが、その神経機構は不明である。そこで本研究課題では、ミツバチを用いて偏光定位行動への時計の関与を行動学的・分子生物学に調べるとともに、脳の偏光情報処理経路における概日時計からの制御機構を神経生理学的に明らかにすることを目的としている。 今年度は、餌場から帰巣する個体を用いて、イソフルラン麻酔によって体内の概日時計の位相を人為的に操作した時の偏光定位行動を調べ、概日時計の行動への関与を検証した。先端に人工餌場のある採餌トンネルを野外に設置し、ミツバチがトンネルを通って人工餌場を訪問するよう訓練し、十分に学習した個体を餌場で捕獲し、一定時間暗黒下で隔離した後に、自作のフライトシミュレータに取り付け、定位する偏光のe-ベクトル方向を解析したところ、麻酔時間によって異なる効果があることがわかった。同様の麻酔処理を施した個体の脳内での時計遺伝子の発現量解析の結果と併せると、比較的長時間の位相操作を行うことで、実時刻でなく概日時計の示す時刻に従って定位方向が変化することが示唆された。 さらに、麻酔が通常の活動リズムに与える影響を評価するため、上記の行動実験と同様な麻酔処理を施した個体を用いて、恒暗条件下で隔離した際の歩行活動のモニタリングを行った。その結果、時計遺伝子の発現リズムとは異なり、実時刻に近い昼行性の活動リズムを示すことが明らかとなった。この理由については不明であり、麻酔方法や活動のモニタリング方法について、引き続き検証中である。


• 昆虫ナビゲーションにおける偏光コンパスの概日時計に基づく時間補償機構

  佐倉 緑, 藍 浩之, 岡田 龍一, 志垣 俊介, 渕側 太郎

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2022 - 31 Mar. 2027


• ミツバチのベクトル統合の神経回路と蜜源ナビゲーションにおける制御機構の解明

  藍 浩之, 岡田 龍一, 佐倉 緑, 池野 英利

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 福岡大学, 01 Apr. 2022 - 31 Mar. 2026


• ミツバチのナビゲーション行動を成熟させる神経メカニズムの解明

  岡田 龍一

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025


• Error avoidance strategy of Platypus quercivorus on their host selection process

  山崎 理正, 伊東 康人, 岡田 龍一, 池野 英利, 森 直樹, 西岡 正恵

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kyoto University, 01 Apr. 2021 - 31 Mar. 2024


• ミツバチのナビゲーションにおける方向と距離の情報統合の脳内メカニズム

  岡田 龍一

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2019 - 31 Mar. 2023

  巣や餌場などの目的地へ移動する「ナビゲーション行動」では、移動中に方向情報と距離情報を逐次取得しそれらを統合して移動を完遂するが、脳内での統合の仕組みについてはほとんどわかっていない。そこで、本研究ではトンネルを用いて採餌訓練をさせたミツバチをバーチャルリアリティ空間内で疑似的に採餌のナビゲーション飛行を再現させ、その時の脳内のナビゲーション中枢の神経活動を記録し、どのように方向情報と距離情報が脳内で統合されるかを明らかにすることを目的としている。 今年度は、昨年度の組織化学的研究で得られた知見を利用して、トンネル内で採餌訓練したミツバチの電気生理実験を行う予定であったが、新型コロナウイルスの影響等からトンネルでの採餌訓練の実施に支障がでた。そのため急遽学内で実施可能な場所を検討し、代替場所にトンネルを設置した。しかし、代替場所で採餌訓練を行ったものの期待したような訓練効果を得ることはできず、時間の浪費を避けるために学内スペースでの採餌訓練を断念した。 その一方で、ミツバチが方向情報を得るために利用している偏光を検出する複眼背側部（DRA）の光に対する応答の記録を行ったところ、DRAは紫外領域の光に特に強く応答をすることがわかった。さらに興味深いことに、この神経応答は記録した時刻によって応答強度が変化した。このことは、ミツバチの偏光情報処理機構に何らかの時間補償機構があることを示唆しており、自然状態でのミツバチの採餌行動の性質を考えると、ナビゲーションの成功の保証を与えている可能性が高い。 現在は、今年度実施できなかった、従来の場所でのトンネル実験の再開と訓練したミツバチでの電気生理実験の準備を進めている。


• Mechanism for detecting the azimuth encoded in the honeybee waggle dance

  AI Hiroyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Fukuoka University, 01 Apr. 2018 - 31 Mar. 2022

  The honeybee waggle dance followers orientate to the dancer not by vision but the antenna, for detecting the azimuth encoded in the dance. On this study we investigated whether both antennae are necessary for the orientation, and what region on the antenna is critical for the orientation by analyzing the waggle dance follower of which local regions of either antenna was covered by a resin on newly emerged adults. As the results we found local coverings of either tip of flagella, middle of flagella or pedicel of one antennae completely defect the orientation to both sides of waggle dancer. These results suggest that the followers orientate the dancer by detecting the difference between somewhat bilateral antennal inputs for deciphering the azimuth.


• Identification of primary attractants for Platypus quercivorus and their application to the control of Japanese oak wilt

  Yamasaki Michimasa

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kyoto University, 01 Apr. 2018 - 31 Mar. 2021

  The ambrosia beetle Platypus quercivorus causes mass mortality of oak trees, Japanese oak wilt, by transporting a pathogenic fungus from trees to trees. Using a flight mill to simulate insect flight in a room, we showed that there are sexual differences in the flight ecology of the beetle, and elucidated the causes of the large variation in its flight distance. Two-choice tests of host and non-host tree saplings showed that the beetle is attracted to volatiles from the canopy of host trees and avoid volatiles from the canopy of non-host trees. It was clarified that the beetle bores into narrow-angled crevices regardless of the type of wood, and that this behavior is activated after flight. Long-term forest dynamics studies in natural and secondary forests clearly showed the effects of Japanese oak wilt, including a decrease in species diversity.


• Neural mechanism of the time compensation for the detection of a navigating direction using the polarized light in the honeybee

  Okada Ryuichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2016 - 31 Mar. 2019

  The central complex is a crucial area in the insect brain for navigation using polarized light. Electrophysiology from the central complex exhibited that some central complex neurons responded to polarized light with a specific e-vector preference. Immunohistochemistry in the central complex was performed against iono- and metabotropic GABA receptors, and showed a different distribution between the two types of receptors. Strong positive signals for the ionotropic GABA receptor were found in the central complex while very few and fair signals were found for the metabotropic GABA receptor. This strongly suggests that ionotropic GABA receptor is dominant for information processing in the central complex compared to metabotropic receptor.


• Dinamical neuronal modeling of insect navigation based on polarization vision

  SAKURA MIDORI, HENZE Miriam J.

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 10 Jul. 2015 - 31 Mar. 2018

  It is well known that many insects deduce their heading direction using polarized skylight. However, it is still unclear how the polarized skylight information, which forms complicate pattern in the sky and even heavily depending on solar azimuths and/or weather conditions, is coded by neural networks in the brain. In this study, we tried to clarify this by dynamical neural modeling using biological and astronomical data. We first analyzed input manners of the polarized light information by a sensor map constructing from the morphological data of the polarized light-detecting area of the compound eye (DRA). Moreover, we tested the accuracy in orientation outputs of the neural network model constructed by the response properties of polarization-sensitive neurons in the brain.


• Brain mechanism

  Okada Ryuichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2013 - 31 Mar. 2016

  To examine neural mechanisms underlying memory formation, the activities of a mushroom body output neuron, PE1 neuron, was analyzed because this neuron shows a learning-related plasticity. However, it is still unknown whether and when PE1 changes its responses during the acquisition period. Using chronic recording from PE1 of a learning bee, we analyzed the spontaneous activity of PE1 between acquisition trials. The spectrum analysis for spontaneous activity showed that the peak of power spectrum of PE1 was shifted to the lower frequency along acquisition trials, suggesting that the inhibition of PE1 spiking activity became stronger than before learning. Next, to reveal inhibitory synaptic connections about PE1, we performed immuno-staining against GABA, iono-, and metabotropic GABA receptors. Immunohistology suggested that PE1 has no GABA-receptors, and thus an indirect neural control might be dominant in an inhibitory connection to PE1.


• Behavioral and electrophysiological study of insect polarization vision by simultaneous recording of polarotactic behavior and brain neural activities.

  Sakura Midori, OKADA Ryuichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2013 - 31 Mar. 2016

  Many insects use polarization pattern of the sky for orientation and navigation. Honeybees show clear polarotaxis to a certain e-vector orientation. In this study, polarotactic behavior of the bee trained to visit an artificial feeder was analyzed. The bees caught at the feeder oriented to the e-vector they experienced during their foraging flight, indicating that they utilized polarized light information from the sky to memorize the food location and to adjust their flight direction. In addition, an extracellular recording method from polarization-sensitive neurons in the bee brain was developed, which allows us to monitor neural responses of a flying tethered bee brain in a flight simulator.


• Effect of social behaviour on carbon budget of honeybee colony under climate change

  MIZUE Ohashi, IKENO Hidetoshi, KIMURA Toshifumi, OKADA Ryuichi, NAKAGIRI Nariyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, University of Hyogo, 01 Apr. 2012 - 31 Mar. 2015

  Honeybee keeps their colony under dramatic change of environment by using social behavior. This study aimed to evaluate honeybee adaptation to environment by measuring energy budget of the hive. The energy budget was estimated from inflow and outflow of carbon from the hive. We found clear daily change of carbon inflow, increasing in evening in summer and increasing noon in autumn. It also decreased from summer to autumn. Total amount of carbon inflow during 3 months was 2.4 kg. Carbon outflow decreased linearly with the decrease of temperature, increasing from summer to autumn. Total amount of the outflow was 0.82 kg.


• Neural mechanisms of motivation-dependent learning and memory

  OKADA Ryuichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Tokushima Bunri University, 2010 - 2012

  To clarify neurophysiologically how motivation influences the efficiency of the learning and memory, I recorded the activities of the brain neurons from honeybees under training for olfactory learning. I have been progressively analyzing data at the level of the neural circuit. With executing experiments, I successfully recorded brain activities in the entire process when olfactory memory is forming. One of honeybee brain neurons, PE1, is known to reduce the responses to learned odor after the memory was absolutely consolidated. I found that the voluntary activity of the PE1 decreased just before forming memory and the first reduction of the odor response was taken place just after the memory was formed, not when the memory was formed..


• 社会性適応のための行動変容機構の研究

  伊藤 悦朗, 池野 英利, 木村 敏文, 岡田 龍一, 大橋 瑞江

  日本学術振興会, 科学研究費助成事業, 特定領域研究, 徳島文理大学, 2008 - 2009

  花から巣に戻ったミツバチは訪れた花の位置(情報)を、「8の字ダンス」という特異的な行動によって巣内のミツバチに伝える。このようなミツバチの社会性の研究は生物学だけからのアプローチでは解明が難しく、工学系研究者との共同研究によって8の字ダンスに挑戦すれば、必ずや世界に先駆けてそのメカニズムを明らかにできると考え、本研究を進めてきた。われわれはミツバチの社会性適応行動を例に取り、特にその中でも他者とのコミュニケーション手段である「8の字ダンス」の基本機序をシステムとして理解を深めながら、社会性適応のための行動変容機構を明らかにすることが本研究課題の目的である。これまで生物データの取得のためにミツバチのダンス行動をビデオ撮影してきた。解析の結果、巣内ミツバチの歩行パターンと1秒あたりの最大移動距離、ダンスの発生場所の分布、ダンス情報の性質、追従バチの追従パターンなど、モデル構築に必要な多くのパラメータを取得した。そこで今年度はこれらのパラメータを利用して、ミツバチの採餌行動のマルコフモデルを構築後、シミュレーション実験を行った。その結果、われわれのモデルはミツバチの採餌行動をよく表現できていること、ダンスによる情報伝達は採餌の効率をあげていることなどがわかった。これらと平行して、ミツバチの行動は巣内外の環境に柔軟に適応して調節されるので巣内外の環境を自動で経時的に計測するためのシステムも同時に構築した。また、効率よく行動パラメータを取得するために、ベクトル量子化法を用いた、ミツバチの自動追跡システムを開発した。これらのことから得られる結果を組み合わせることで、ダンス行動によるコロニー維持の効果や情報の伝搬様式などのより深い理解につながると期待される。