Research activity information

• Evolution of wheat blast resistance gene Rmg8 accompanied by differentiation of variants recognizing the powdery mildew fungus.

  Asuke, S, Morita, K, Shimizu, M, Abe, F, Terauchi. R, Nago, C, Takahashi, Y, Shibata, M, Yoshioka, M, Iwakawa, M, Kishi-Kadoshi, M, Su, Z, Nasuda, S, Handa, H, Fujita, M, Tougou, M, Hatta, K, Mori, N, Matsuoka, Y, Kato, K, Tosa, Y

  Jul. 2024, Nature plants, 10, 971 - 983, English

  [Refereed]


• Testing the genetic autonomy of the plasmon in the Triticum–Aegilops complex: The final work of Prof. Koichiro Tsunewaki

  Naoki Mori

  International Society of Cytology, Sep. 2023, CYTOLOGIA, 88(3) (3), 177 - 181

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Geographical distribution and adaptive variation of VRN-A3 alleles in worldwide polyploid wheat (Triticum spp.) species collection

  Kazusa Nishimura, Hirokazu Handa, Naoki Mori, Kanako Kawaura, Akira Kitajima, Tetsuya Nakazaki

  MAIN CONCLUSION: The distribution of early flowering alleles of VRN-A3 was found to be biased to low latitudes, and these alleles may contribute to environmental adaptability to low latitudes in cultivated emmer wheat. In wheat (Triticum spp.), the flowering time is an important trait for successful seed production and yield by adapting to the regional environment. An early flowering allele of VRN-A3 with 7- and 25-bp insertions in the promoter region (Vrn-A3a-h1) has recently been reported from the analysis of an emmer wheat (Triticum turgidum L. ssp. dicoccum) accession, TN26. This early flowering allele of VRN-A3 might be associated with the regional adaptation of wheat. In this study, we elucidated its geographic distribution to assess the importance of the early flowering allele of VRN-A3 in worldwide wheat collection. From sequence analysis, we identified six VRN-A3 alleles with the 7- and 25-bp insertions, namely, Vrn-A3a-h2, Vrn-A3a-h3, Vrn-A3a-h4, Vrn-A3a-h5, Vrn-A3a-h6, and Vrn-A3c-h2 from wild emmer wheat, while we identified two VRN-A3 alleles with these insertions, Vrn-A3a-h2 and Vrn-A3c-h1 from cultivated tetraploid and hexaploid wheat species in addition to Vrn-A3a-h1. Among VRN-A3 alleles distributed in cultivated wheat, we found that Vrn-A3a-h2 promoted early heading, whereas Vrn-A3c-h1 did not affect heading time. Our analysis showed that the distribution of early flowering alleles of VRN-A3 dominated in cultivated emmer wheat in Ethiopia and India, which actually showed an early flowering phenotype. This implied that the early flowering alleles of VRN-A3 contribute to adaptability to a low-latitude environment in cultivated emmer wheat. We could not find durum (T. turgidum L. ssp. durum) and bread wheat (T. aestivum L. ssp. aestivum) accessions with these early flowering alleles. Our findings indicated that Vrn-A3a-h1 and Vrn-A3a-h2 were useful for breeding of early flowering cultivars in durum and bread wheat varieties.

  Springer Science and Business Media LLC, Jun. 2021, Planta, 253(6) (6), 132 - 132, English, International magazine

  [Refereed]

  Scientific journal


• Reproductive and genetic roles of the maternal progenitor in the origin of common wheat ( Triticum aestivum L.)

  Yoshihiro Matsuoka, Naoki Mori

  Common wheat (Triticum aestivum L., AABBDD genome) is thought to have emerged through natural hybridization between Triticum turgidum L. (AABB genome) and Aegilops tauschii Coss. (DD genome). Hybridization barriers and doubling of the trihaploid F1 hybrids' genome (ABD) via unreduced gamete fusion had key roles in the process. However, how T. turgidum, the maternal progenitor, was involved in these mechanisms remains unknown. An artificial cross-experiment using 46 cultivated and 31 wild T. turgidum accessions and a single Ae. tauschii tester with a very short genetic distance to the common wheat D genome was conducted. Cytological and quantitative trait locus analyses of F1 hybrid genome doubling were performed. The crossability and ability to cause hybrid inviability did not greatly differ between the cultivars and wild accessions. The ability to cause hybrid genome doubling was higher in the cultivars. Three novel T. turgidum loci for hybrid genome doubling, which influenced unreduced gamete production in F1 hybrids, were identified. Cultivated T. turgidum might have increased the probability of the emergence of common wheat through its enhanced ability to cause genome doubling in F1 hybrids with Ae. tauschii. The ability enhancement might have involved alterations at a relatively small number of loci.

  Last, Wiley, Dec. 2020, Ecology and Evolution, 10(24) (24), 13926 - 13937, English, International magazine

  [Refereed]

  Scientific journal


• Rhizospheric bacterial community structure of Triticum and Aegilops revealed by pyrosequencing analysis of the 16S rRNA gene: dominance of the A genome over the B and D genomes

  Natsumi Ito, Naoki Mori, Naohiko T. Miyashita

  This study examined the relationship between host plant and rhizospheric bacterial community structure, including composition and diversity, in Triticum and Aegilops species (12 and two accessions, respectively) as well as three closely related species, barley, rye and oat (four accessions), to explore the possibility that wheat root and rhizosphere interaction can be utilized for wheat breeding and biotechnology in the future. For this purpose, DNA was isolated from rhizospheric soil samples and one control non-rhizospheric soil sample, and the 16S rRNA gene region was amplified and subjected to DNA pyrosequencing. A total of 132,888 amplicons were analyzed. Bacterial composition at the phylum level was similar among the 18 rhizospheric samples; however, the proportion of Acidobacteria was much lower in these samples than in the control non-rhizospheric soil sample, indicating that rhizospheres influenced the bacterial composition even at the higher taxonomic level. Across host plant genome types (three levels of ploidy and three major genomes, A, B and D), there was no detectable difference in phylum composition or species diversity. Estimated bacterial species diversity was higher in the control soil sample than in plant rhizospheric soils, implying that bacterial species diversity was reduced in rhizospheres. A PCoA plot and UPGMA dendrogram based on the bacterial species composition showed that control soil was distantly located from the plant rhizospheric samples and that Triticum, Aegilops and related species were well separated. PERMANOVA analysis detected statistically significant differentiation among these four groups. Clustering of Triticum species suggested that the A genome was dominant over the B and D genomes, with respect to the influence on rhizospheric bacterial species composition. Although the cause was not investigated in this study, these results clearly indicated that the genetic constitution of the plant host exerted a strong influence on rhizospheric bacterial community structure.

  Genetics Society of Japan, Oct. 2020, Genes & Genetic Systems, 95(5) (5), 249 - 268, English

  [Refereed]

  Scientific journal


• Experimental evolutionary studies on the genetic autonomy of the cytoplasmic genome ‘Plasmon’ in the Triticum (wheat)-Aegilops complex

  Koichiro Tsunewaki, Naoki Mori, TAKUMI SHIGEO

  The term ‘plasmon’ is used to indicate the whole cytoplasmic genetic system, whereas ‘genome’ refers to the whole nuclear genetic system. Although maternal inheritance of the plasmon is well documented in Angiosperms, its genetic autonomy from the coexisting nuclear genome still awaits critical examination. We aimed to test this autonomy in two related studies: one was to deter

  the National Academy of Sciences of the United States of America, Feb. 2019, Proceedings of the National Academy of Sciences of the United States of America, 116(8) (8), 3082 - 3090, English

  [Refereed]

  Scientific journal


• Evaluation of grain dimension and weight using backcross recombinant inbred lines between wild and domesticated emmer wheat

  Miyazakki Yuki, Ngoc Pham M, Liberatore, Katie L, Kianian, Shahryar F, Vladutu, Cristian I, Mori Naoki

  Emmer wheat (Triticum turgidum ssp. dicoccum) represents the primitive situation in the domestication of AABB tetraploid wheat. As one of the earliest domesticated grain species, it was a principal crop in the development and spread of Neolithic agriculture in the Old World. Grain weight and dimension (size
  and shape) have been major targets of selection since the beginning of

  The Society of Crop Science and Breeding in kinki, Japan, Nov. 2017, Journal of Crop Research, 62, 31 - 36, English

  [Refereed]

  Scientific journal


• Chloroplast DNA haplotype variation within two natural populations of wild emmer wheat (Triticum turgidum ssp. dicoccoides) in southern Turkey

  Takako Shizuka, Naoki Mori, Hakan Ozkan, Shoji Ohta

  May 2015, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 29(3) (3), 423 - 430, English

  [Refereed]

  Scientific journal


• Mapping QTLs for cold tolerance at germination and the early seedling stage in rice (Oryza sativa L.)

  Aloka Lanka Ranawake, Oliver Escano Manangkil, Shinya Yoshida, Takashi Ishii, Naoki Mori, Chiharu Nakamura

  Nov. 2014, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 28(6) (6), 989 - 998, English

  [Refereed]

  Scientific journal


• Genetic effect of the Aegilops caudata plasmon on the manifestation of the Ae. cylindrica genome

  Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

  Oct. 2014, GENES & GENETIC SYSTEMS, 89(5) (5), 195 - 202, English

  [Refereed]

  Scientific journal


• Rediscovery of Indian dwarf wheat (Triticum aestivum L. ssp. sphaerococcum (Perc.) MK.) an ancient crop of the Indian subcontinent

  Naoki Mori, Shoji Ohta, Hajime Chiba, Toshiya Takagi, Yukiko Niimi, Vasant Shinde, Mukund D. Kajale, Toshiki Osada

  Springer, Aug. 2013, Genetic Resources and Crop Evolution, 60(6) (6), 1771 - 1775, English

  [Refereed]

  Scientific journal


• Mapping of quantitative trait loci controlling seedling vigor in rice (Oryza sativa L.) under submergence

  O. E. Manangkil, H. T. T. Vu, N. Mori, S. Yoshida, C. Nakamura

  Jul. 2013, EUPHYTICA, 192(1) (1), 63 - 75, English

  [Refereed]

  Scientific journal


• Molecular Genetic Analysis of domestication traits in emmer wheat. I: Map construction and QTL analysis using an F-2 population.

  Phan Thi Thanh, Cristian Ioan Vladutu, Shahryar F. Kianian, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Naoki Mori

  Apr. 2013, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 27(2) (2), 3627 - 3637, English

  [Refereed]

  Scientific journal


• A possible breakage of linkage disequilibrium between mitochondrial and chloroplast genomes during Emmer and Dinkel wheat evolution

  Mai Tsujimura, Naoki Mori, Hiroshi Yamagishi, Toru Terachi

  Apr. 2013, GENOME, 56(4) (4), 187 - 193, English

  [Refereed]

  Scientific journal


• INDUCTION AND REPRESSION OF GENE EXPRESSION MEDIATING ETHYLENE BIOSYNTHESIS AND SODIUM/PROTON EXCHANGE IN RICE SEEDLINGS UNDER SUBMERGENCE STRESS

  Hien Thi Thu Vu, Oliver Escano Manangkil, Naoki Mori, Shinya Yoshida, Chiharu Nakamura

  Jun. 2012, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 26(3) (3), 2945 - 2951, English

  [Refereed]

  Scientific journal


• EXPRESSION PROFILES OF STRESS RESPONSIVE GENES IN RICE (ORYZA SATIVA L.) UNDER ABIOTIC STRESSES

  Aloka Lanka Ranawake, Naoki Mori, Chiharu Nakamura

  Apr. 2012, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 26(2) (2), 2838 - 2843, English

  [Refereed]

  Scientific journal


• 葉緑体DNAの変異からみたインド亜大陸のエンマーコムギとインド矮性コムギの遺伝的多様性と起源について

  MORI NAOKI, Takagi Toshiya, Chiba Hajime, Ohta Shoji

  インド亜大陸で数千年にわたって栽培されてきたエンマーコムギとインド矮性コムギの起源と伝播を明らかにするため、母性遺伝する葉緑体DNAのマイクロサテライト多型を詳細に解析した。その結果、現在インドで伝統的に栽培されているエンマーコムギは予想に反してアラビア海の交易とともに海のルートで北アフリカから伝播した可能性が高いことが明らかになった。また、フィールドでの現地調査から、全世紀半ばにその栽培記録が途絶えていたインド矮性コムギが現在でも栽培されていることが再発見された。

  Apr. 2012, 環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2010-11年度成果報告書, 73 - 84, Japanese

  Research institution


• インドにおけるエンマーコムギの栽培と利用—現状とその意味すること

  大田 正次, MORI NAOKI, 千葉 一

  インダス文明期から栽培さされてきたエンマーコムギの栽培と利用を現地における調査から明らかにするため、インド南部のニルギリ高原からカルナータカ州を経てマハーラーシュトラ州にかけて踏査した。この結果をもとにエンマーコムギの伝統的栽培とその利用について報告する。

  総合地球環境学研究所, Apr. 2012, 「環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2010-11年度成果報告書, 65 - 72, Japanese

  Research institution


• Differential expression Nilaparvata lugens (Stål) (Homoptera: Delphacidae) with different levels of virulence.

  Nirosha Samanmali Gamalath, Muhammad Tufail, Prem Nidhi Sharma, Naoki Mori, Makio Takeda, Chiharu Nakamura

  Apr. 2012, Entomol. Zool., 47, 9 - 16, English

  [Refereed]

  Scientific journal


• 葉緑体DNAの変異からみたインド亜大陸のエンマーコムギとインド矮性コムギの遺伝的多様性と起源について

  Mori Naoki, Takagi Toshiya, Chiba Hajime, Ohta Shoji

  総合球環境学研究所, Feb. 2012, 「環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2010-11年度成果報告書, H03, 73 - 84, Japanese

  Research institution


• インドにおけるエンマーコムギの栽培と利用—現状とその意味すること

  Ohta Shoji, Mori Naoki, Chiba Hajime

  総合地球環境学研究所, Feb. 2012, 総合地球環境学研究所プロジェクトH-03 「環境変化とインダス文明」2010-11年度成果報告書, H03, 65 - 72, Japanese

  Research institution


• Differential expression of vitellogenin mRNA and protein in response to rice resistance genes in two strains of Nilaparvata lugens (Hemiptera: Delphacidae) with different levels of virulence

  Nirosha Samanmali Gamalath, Muhammad Tufail, Prem Nidhi Sharma, Naoki Mori, Makio Takeda, Chiharu Nakamura

  Feb. 2012, APPLIED ENTOMOLOGY AND ZOOLOGY, 47(1) (1), 9 - 16, English

  [Refereed]

  Scientific journal


• QTL analysis for submergence avoidance using backcross recombinant inbred lines between Oryza sativa and O. rufipogon.

  Phuong Dang, Thai Phan, Pham Thien Thanh, Naoki Mori, Ryo Ishikawa, Takashige Ishii

  Backcross populations are useful for evaluating the agronomic traits from unadapted germplasms. In this study, we produced 159 and 170 backcross recombinant inbred lines (BRILs) at the BC₂F₈ generation between Oryza sativa Japonica Nipponbare (a recurrent parent) and O. rufipogon W630 and between O. sativa Indica IR36 (a recurrent parent) and O. rufipogon W630, respectively. These BRILs were found to have nearly homozygous genome constitutions based on the marker genotypes at SSR loci covering whole chromosomes. Since their selfed progenies have almost identical genotypes in each line, the simple test tube method was applied for BRILs (BC₂F₉ generation) to evaluate the ability of fast shoot elongation after germination stage under submerged condition. Then, QTL analysis of submergence avoidance was carried out with marker genotype data. As a result, three (on chromosomes 2, 3 and 10) and two QTLs (on chromosomes 3 and 4) were found in the BRILs having genetic backgrounds of Nipponbare and IR36, respectively. Of these, one each had the wild allele with increasing effect on shoot length. These favorable wild alleles would be useful in improving direct-seeding cultivation in water-prone areas.

  The Society of Crop Science and Breeding in Kinki, Japan, 2011, Journal of Crop research, 56, 45 - 49, English

  [Refereed]

  Scientific journal


• Comparative nucleotide sequence analysis of the D genome-specific sequence-tagged-site locus A1 in Triticum aestivum and its implication for the origin of subspecies sphaerococcum

  Nobuaki Asakura, Naoki Mori, Chiharu Nakamura, Ichiro Ohtsuka

  2011, Breeding Science, 61(2) (2), 212 - 216, English

  [Refereed]

  Scientific journal


• Development of backcross recombinant inbred lines between Oryza sativa Nipponbare and O. rufipogon and QTL detection on drought tolerance

  Pham Thien Thanh, Phuong Dang Thai Phan, Naoki Mori, Ryo Ishikawa, Takashige Ishii

  Jpn Soc. Plant Breed., 2011, Breeding Science, 61(1) (1), 76 - 79, English

  [Refereed]

  Scientific journal


• Post-germination seedling vigor under submergence and submergence-induced SUB1A gene expression in indica and japonica rice (Oryza sativa L.)

  H. T. T. Vu, O. E. Manangkil, N. Mori, S. Yoshida, C. Nakamura

  Jun. 2010, AUSTRALIAN JOURNAL OF CROP SCIENCE, 4(4) (4), 264 - 272, English

  [Refereed]

  Scientific journal


• 生業システム研究グループ2009年度活動報告

  大田 正次, 千葉 一, MORI NAOKI, 三浦 励一

  環境変化とインダス文明の関係を遺跡から出土する植物遺物をもとにした古民族植物学的研究と現存する栽培植物の利用をもとにした民族植物学的研究の両面から探ることを目的に、インド中南部においてフィールド調査を行った。

  Apr. 2010, 「環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2009年度成果報告書, 93 - 102, Japanese

  Research institution


• Post-germination seedling vigor under submergence and submergence-induced SUB1A gene expression in indica and japonica rice (Oryza sativa L.)

  H. T. T. Vu, O. E. Manangkil, Naoki Mori, S. Yoshida, C. Nakamura

  We evaluated post-germination seedling vigor in indica and japonica rice under submergence based on shoot elongation and recovery after submergence. The japonica cultivars showed more vigorous shoot elongation than indica cultivars under submergence in the dark, whereas an opposite response was observed under the light-dark submergence. Both Nipponbare and FR13A that are respec

  2010, Australian J. Crop Science, 4(4) (4), 264 - 272, English

  [Refereed]

  Scientific journal


• Genetic variation in domesticated emmer wheat (Triticum turgidum L.) in and around Abyssinian highlands

  Shotaro Takenaka, Naoki Mori, Taihachi Kawahara

  2010, Breeding Science, 60(3) (3), 212 - 227, English

  [Refereed]

  Scientific journal


• Genome size variation in diploid and tetraploid wild wheats

  Hakan Ozkan, Metin Tuna, Benjamin Kilian, Naoki Mori, Shoji Ohta

  2010, AOB PLANTS, 1 - 11 (plq015), English

  [Refereed]

  Scientific journal


• Genetic diversity and origin of timopheevi wheat inferred by chloroplast DNA fingerprinting

  Naoki Mori, Yasutaka Kondo, Takashige Ishii, Taihachi Kawahara, Jan Valkoun, Chiharu Nakamura

  Dec. 2009, BREEDING SCIENCE, 59(5) (5), 571 - 578, English

  [Refereed]

  Scientific journal


• SUBMERGENCE-INDUCED ADH AND ALDH GENE EXPRESSION IN JAPONICA AND INDICA RICE WITH CONTRASTING LEVELS OF SEEDLING VIGOR UNDER SUBMERGENCE STRESS

  H. T. T. Vu, O. E. Manangkil, N. Mori, S. Yoshida, C. Nakamura

  Nov. 2009, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 23(4) (4), 1469 - 1473, English

  [Refereed]

  Scientific journal


• Differential cDNA-AFLP screening of transcripts associated with brown planthopper resistance in rice (Oryza sativa L.)

  N. S. Gamalath, P. N. Sharma, N. Mori, C. Nakamura

  Jul. 2009, AUSTRALIAN JOURNAL OF CROP SCIENCE, 3(4) (4), 201 - 206, English

  [Refereed]

  Scientific journal


• Characterization of brown planthopper strains based on their differential responses to introgressed resistance genes and on mitochondrial DNA polymorphism

  Muhammad NAEEMULLAH, Prem N. SHARMA, Muhammad TUFAIL, MORI NAOKI, Masaya MATSUMURA, Makio TAKEDA, Chiharu NAKAMURA

  Biotypes cause a serious problem in controlling brown planthopper (BPH), Nilaparvata lugens Stål (Homoptera: Delphacidae).
  We characterized four BPH strains, which were collected in different localities in Japan and maintained by
  random mating within each strain for different periods. The virulence of the BPH strains was evaluated based on their
  postembryonic development on sus

  JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY, May 2009, Appl. Entomol. Zool., 44(3) (3), 475 - 483, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species

  Naoki Mori, Yuko Hidehira, Takashige Ishii, Chiharu Nakamura

  The domestication of wheat and barley was the most important step in the emergence of farming communities that led to the ancient civilizations of Mesopotamia. Several lines of evidence indicate that emmer wheat was the earliest form of domesticated wheat derived from wild emmer wheat. Chloroplast DNA fingerprinting of wild and domesticated emmer wheat revealed that two dis

  2009, Proc. 4th International Conference on Energy Efficiency and Agricultural Engineering, 725 - 729, English

  International conference proceedings


• Genotyping of the Q locus in wheat by a simple PCR-RFLP method

  Nobuaki Asakura, Naoki Mori, Chiharu Nakamura, Ichiro Ohtsuka

  Jpn Soc. Genetics, 2009, Genes and Genetic Systems, 84(3) (3), 233 - 237, English

  [Refereed]

  Scientific journal


• A simple, rapid and reliable bioassay for evaluating seedling vigor under submergence in indica and japonica rice (Oryza sativa L.)

  Oliver E. Manangkil, Hien Thi Thu Vu, Shinya Yoshida, Naoki Mori, Chiharu Nakamura

  Sep. 2008, EUPHYTICA, 163(2) (2), 267 - 274, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mapping of quantitative trait loci associated with cold tolerance at the post-germination stage in rice

  A. L. Ranawake, T. Ishii, N. Mori, S. Yoshida, C. Nakamura

  Feb. 2008, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 22(1) (1), 536 - 540, English

  [Refereed]

  Scientific journal


• Sequence diversity and copy number variation of Mutator-like transposases in wheat

  Nobuaki Asakura, Shinya Yoshida, Naoki Mori, Ichiro Ohtsuka, Chiharu Nakamura

  Brazilian Journal of Genetics, 2008, Genetics and Molecular Biology, 31(2) (2), 539 - 546, English

  [Refereed]

  Scientific journal


• Mitochondrial microsatellite variability in common wheat and its ancestral species

  Takashige Ishii, Chisa Takahashi, Nobuyuki Ikeda, Osamu Kamijima, Naoki Mori

  Jun. 2006, GENES & GENETIC SYSTEMS, 81(3) (3), 211 - 214, English

  [Refereed]

  Scientific journal


• Chloroplast and nuclear microsatellite analysis of Aegilops cylindrica

  HT Gandhi, MI Vales, CJW Watson, CA Mallory-Smith, N Mori, M Rehman, RS Zemetra, O Riera-Lizarazu

  Aug. 2005, THEORETICAL AND APPLIED GENETICS, 111(3) (3), 561 - 572, English

  [Refereed]

  Scientific journal


• Genealogical use of chloroplast DNA variation for intraspecific studies of Aegilops tauschii Coss.

  Y Matsuoka, N Mori, T Kawahara

  Jul. 2005, THEORETICAL AND APPLIED GENETICS, 111(2) (2), 265 - 271, English

  [Refereed]

  Scientific journal


• Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome

  Y Ogihara, Y Yamazaki, K Murai, A Kanno, T Terachi, T Shiina, N Miyashita, S Nasuda, C Nakamura, N Mori, S Takumi, M Murata, S Futo, K Tsunewaki

  2005, NUCLEIC ACIDS RESEARCH, 33(19) (19), 6235 - 6250, English

  [Refereed]

  Scientific journal


• Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by AFLP and nuclear and chloroplast SSR markers

  Z Hashimoto, N Mori, M Kawamura, T Ishii, S Yoshida, M Ikegami, S Takumi, C Nakamura

  Nov. 2004, THEORETICAL AND APPLIED GENETICS, 109(8) (8), 1586 - 1596, English

  [Refereed]

  Scientific journal


• Chloroplast and nuclear DNA variation in common wheat: insight into the origin and evolution of common wheat

  S Hirosawa, S Takumi, T Ishii, T Kawahara, C Nakamura, N Mori

  Oct. 2004, GENES & GENETIC SYSTEMS, 79(5) (5), 271 - 282, English

  [Refereed]

  Scientific journal


• Genetic diversity and phylogenetic relationships in Japanese Sake-brewing and staple rice cultivars as revealed by AFLP and SSRP analyses.

  Z. Hashimoto, N. Mori, M. Kawamura, T. Ishii, S. Yoshida, W. Ikegami, S. Takumi, C. Nakamura

  Sep. 2004, Theoretical and Applied Genetics, 109, 1586 - 1596

  [Refereed]

  Scientific journal


• Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus

  Y Tosa, K Hirata, H Tamba, S Nakagawa, Chuma, I, C Isobe, J Osue, AS Urashima, LD Don, M Kusaba, H Nakayashiki, A Tanaka, T Tani, N Mori, S Mayama

  May 2004, PHYTOPATHOLOGY, 94(5) (5), 454 - 462, English

  Scientific journal


• Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stal) resistance genes Bph1 and Bph2 on rice chromosome 12

  PN Sharma, A Torii, S Takumi, N Mori, C Nakamura

  Feb. 2004, HEREDITAS, 140(1) (1), 61 - 69, English

  [Refereed]

  Scientific journal


• Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus

  Y. Tosa, K. Hirata, H. Tamba, S. Nakagawa, I. Chuma, C. Isobe, J. Osue, A. S. Urashima, L. D. Don, M. Kusaba, H. Nakayashiki, A. Tanaka, T. Tani, N. Mori, S. Mayama

  American Phytopathological Society, 2004, Phytopathology, 94(5) (5), 454 - 462, English

  Scientific journal


• Marker-assisted pyramiding of two brown planthopper (Nolaparvata lugens Stal) resistance genes Bph1 and Bph2 in rice (Oryza genes Bph1 and Bph2 in rice (Oryza sativa L.)

  SHARMA P N, TORII A, TAKUMI Shigeo, MORI Naoki, NAKAMURA Chiharu

  2004, Hereditas, 140: 61-69, English

  [Refereed]

  Scientific journal


• RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stal) resistance gene Bph1 in rice

  N Sharma, Y Ketipearachchi, K Murata, A Torii, S Takumi, N Mori, C Nakamura

  2003, EUPHYTICA, 129(1) (1), 109 - 117, English

  [Refereed]

  Scientific journal


• Transferability of hexaploid wheat (Triticum aestivum) microsatellite markers to tetraploid species (T-turgidum) carrying A and B genomes

  NAB Jelodar, N Mori, C Nakamura

  2003, CEREAL RESEARCH COMMUNICATIONS, 31(3-4) (3-4), 273 - 280, English

  Scientific journal


• Towards molecular cloning of resistance genes against brown planthopper (Nilaparvata lugens Stal) in rice: a case study of natural insect resistance genes.

  SHARMA P N, NURATA K, TORII A, TAKUMI Shigeo, MORI Naoki, NAKAMURA Chiharu

  2003, Trends in Entomol., 未記入, English

  Scientific journal


• Tauschii Core Collection; an effective tool for utilize wheat D genome genetic resources.

  KAWAHARA T, TAKUMI Shigeo, MATSUOKA Y, MORI Naoki, YASUI Y

  2003, Xth Int. WheatGenet. Symp., Paestum, Vol.2:584-586, English

  Scientific journal


• Origins of domesticated emmer and common wheat inferred from chloroplast DNA fingerprinting.

  MORI Naoki, ISHII Takashige, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA Y

  2003, Proc. Xth Int. Wheat Genet. Symp., Vol.1,pp.25-28, English

  Scientific journal


• Origin of domesticated emmer and common wheat inferred from chloroplast DNA finger printing.

  MORI Naoki, ISHII T, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA S, NAKAMURA Chiharu

  2003, Xth Int. Wheat Genet. Symp.Paestum, Vol.1:25-28, English

  Scientific journal


• Mitochondrial genome and genes of common wheat, Triticum aestivum cv. Chinese Spring.

  OGIHARA Y, FUTO S, KANNO A, MIYASHITA N, NASUDA S, SHIINA T, TERAUCHI T, GUO C-H, NAKAMURA Chiharu, MORI Naoki, TAKUMI Shigeo, MURATA M, YAMAZAKI Y, MURAI K, MATSUOKA Y, TSUNEWAKI K

  2003, Xth Int. Wheat Genet. Symp.Paestum, Vol.3:1020-1022, English

  Scientific journal


• Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA

  Y Ogihara, K Isono, T Kojima, A Endo, M Hanaoka, T Shiina, T Terachi, S Utsugi, M Murata, N Mori, S Takumi, K Ikeo, T Gojobori, R Murai, K Murai, Y Matsuoka, Y Ohnishi, H Tajiri, K Tsunewaki

  Jan. 2002, MOLECULAR GENETICS AND GENOMICS, 266(5) (5), 740 - 746, English

  Scientific journal


• Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species

  T. Ishii, N. Mori, Y. Ogihara

  2001, Theoretical and Applied Genetics, 103(6-7) (6-7), 896 - 904, English

  [Refereed]

  Scientific journal


• Single nucleotide polymorphisms in an STS region linked to the Ncc-tmp1A locus are informative for characterizing the differentiation of chromosome 1A in wheat

  Nobuaki Asakura, Naoki Mori, Takao Ishido, Ichiro Ohtsuka, Chiharu Nakamura

  2001, Genes and Genetic Systems, 76(5) (5), 295 - 304, English

  [Refereed]

  Scientific journal


• Assignment of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph4 to the rice chromosome 6

  Mitsuhiro Kawaguchi, Kazumasa Murata, Takashige Ishii, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

  2001, Breeding Science, 51(1) (1), 13 - 18

  [Refereed]

  Scientific journal


• Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2

  H. Murai, Z. Hashimoto, P. N. Sharma, T. Shimizu, K. Murata, S. Takumi, N. Mori, S. Kawasaki, C. Nakamura

  2001, Theoretical and Applied Genetics, 103(4) (4), 526 - 532

  [Refereed]

  Scientific journal


• Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12

  K. Murata, M. Fujiwara, H. Murai, S. Takumi, N. Mori, C. Nakamura

  2001, Cereal Research Communications, 29(3-4) (3-4), 245 - 250

  [Refereed]

  Scientific journal


• Molecular cloning of three homoeologous cDNAs encoding orthologs of the maize KNOTTED1 homeobox protein from young spikes of hexaploid wheat

  Shigeo Takumi, Takayuki Kosugi, Koji Murai, Naoki Mori, Chiharu Nakamura

  May 2000, Gene, 249(1-2) (1-2), 171 - 181, English

  [Refereed]

  Scientific journal


• A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'

  Sergei Tsvetanov, Ryoko Ohno, Kanako Tsuda, Shigeo Takumi, Naoki Mori, Atanas Atanassov, Chiharu Nakamura

  Feb. 2000, Genes and Genetic Systems, 75(1) (1), 49 - 57

  [Refereed]

  Scientific journal


• Chinese Spring Wheat (Triticum aestivum L.) Chloroplast Genome: Complete Sequence and Contig Clones

  Yasunari Ogihara, Kazuhiro Isono, Toshio Kojima, Akira Endo, Mitsumasa Hanaoka, Takashi Shiina, Toru Terachi, Shigeko Utsugi, Minoru Murata, Naoki Mori, Shigeo Takumi, Kazuho Ikeo, Takashi Gojobori, Rika Murai, Koji Murai, Yoshihiro Matsuoka, Yukari Ohnishi, Hikaru Tajiri, Koichiro Tsunewaki

  2000, Plant Molecular Biology Reporter, 18(3) (3), 243 - 253

  [Refereed]

  Scientific journal


• Identification of paternal mitochondrial DNA sequences in the nucleus-cytoplasm hybrids of tetraploid and hexaploid wheat with D and D2 plasmons from Aegilops species

  Naoko Tsukamoto, Nobuaki Asakura, Nobuaki Hattori, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

  2000, Current Genetics, 38(4) (4), 208 - 217

  [Refereed]

  Scientific journal


• Nicotiana tabacum cDNAs encoding α and β subunits of a heterotrimeric GTP-binding protein isolated from hairy root tissues

  S. Ando, S. Takumi, Y. Ueda, T. Ueda, N. Mori, C. Nakamura

  2000, Genes and Genetic Systems, 75(4) (4), 211 - 221

  [Refereed]

  Scientific journal


• New members of a cold-responsive group-3 Lea/Rab-related Cor gene family from common wheat (Triticum aestivum L.)

  Kanako Tsuda, Sergei Tsvetanov, Shigeo Takumi, Naoki Mori, Atanas Atanassov, Chiharu Nakamura

  2000, Genes and Genetic Systems, 75(4) (4), 179 - 188

  [Refereed]

  Scientific journal


• Mapping of QTLs for low temperature response in seedlings of rice (Oryza sativa L.)

  S Misawa, N Mori, S Takumi, S Yoshida, C Nakamura

  2000, CEREAL RESEARCH COMMUNICATIONS, 28(1-2) (1-2), 33 - 40, English

  [Refereed]

  Scientific journal


• Variations in the gliadin composition in immature embryo culture derived somaclonal lines of durum wheat

  Naoko Yokouchi, Nobuaki Asakura, Sergei Tsvetanov, Naoki Mori, Atanas Atanassov, Chiharu Nakamura

  Japanese Society for Plant Cell and Molecular Biology, 1999, Plant Biotechnology, 16(2) (2), 167 - 170, English

  [Refereed]

  Scientific journal


• Segregation distortion through female gametophytes in interspecific hybrids of tetraploid wheat as revealed by RAPD analysis

  Mizuwa Manabe, Tomoko Ino, Masao Kasaya, Shigeo Takumi, Naoki Mori, Ichiro Ohtsuka, Chiharu Nakamura

  1999, Hereditas, 131(1) (1), 47 - 53

  [Refereed]

  Scientific journal


• Ac-mediated trans-activation of the Ds element in rice (Oryza sativa L.) cells as revealed by GUS assay

  Renando Solis, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

  1999, Hereditas, 131(1) (1), 23 - 31

  [Refereed]

  Scientific journal


• Variations in the maize Ac transposase transcript level and the Ds excision frequency in transgenic wheat callus lines

  Shigeo Takumi, Koji Murai, Naoki Mori, Chiharu Nakamura

  1999, Genome, 42(6) (6), 1234 - 1241

  [Refereed]

  Scientific journal


• RFLP mapping of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph2 of indica rice introgressed into a Japonica breeding line 'Norin- PL4'

  Kazumasa Murata, Manabu Fujiwara, Chukichi Kaneda, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

  Dec. 1998, Genes and Genetic Systems, 73(6) (6), 359 - 364

  [Refereed]

  Scientific journal


• Salinity tolerance in Triticum aestivum-Lophopyrum elongatum amphiploid and 5E disomic addition line evaluated by NaCl effects on photosynthesis and respiration

  Kazue Kasai, Hiroshi Fukayama, Naotsugu Uchida, Naoki Mori, Takeshi Yasuda, Yoshikiyo Oji, Chiharu Nakamura

  Springer Science and Business Media LLC, Sep. 1998, Cereal Research Communications, 26(3) (3), 281 - 287

  [Refereed]

  Scientific journal


• MOLECULAR CLONING AND CHARACTERIZATION OF cDNAs PREFERENTIALLY EXPRESSED DURING THE DEVELOPMENT OF HAIRY ROOTS AND NORMAL ROOTS IN TOBACCO(Nicotiana tabacum L. )

  NAKAMURA Chiharu, NISHIMURA Nobuyuki, MISHIMA Masafumi, UEDA Tadamasa, ONAKA Takashi, TSVETANOV Sergei, TAKUMI Shigeo, MORI Naoki

  Last, May 1998, Plant and cell physiology, 39, S65 - S65, English

  [Refereed]


• Production and characterization of transgenic indica rice plants carrying maize Ac-Ds elements introduced by particle bombardment

  Renando Solis, Shinya Yoshida, Naoki Mori, Chiharu Nakamura

  Japanese Society for Plant Cell and Molecular Biology, 1998, Plant Biotechnology, 15(2) (2), 87 - 93, English

  [Refereed]

  Scientific journal


• RFLP analysis of nuclear DNA for study of phylogeny and domestication of tetraploid wheat

  Naoki Mori, Takahiro Moriguchi, Chiharu Nakamura

  Jun. 1997, Genes and Genetic Systems, 72(3) (3), 153 - 161, English

  [Refereed]

  Scientific journal


• Variation in coxII intron in the wild ancestral species of wheat

  Naoki Mori, Naohiko T. Miyashita, Toru Terachi, Chiharu Nakamura

  1997, Hereditas, 126(3) (3), 281 - 288, English

  [Refereed]

  Scientific journal


• Genetic Diversity and Subspecies Differentiation in Local Rice Cultivars from Manipur State of India

  Kaneda Chukichi, Umikawa Masato, Smgh M Rohinikumar, Nakamura Chiharu, Mori Naoki

  The Manipur State of India is considered as a part of the center of diversity of Asian rice. To obtain information on the genetic diversity and subspecies differentiation in local rice cultivars from the area, a survey was made of selected morphological and physiological traits, chloroplast DNA (CtDNA) type, and total genomic DNA polymorphism. Judging from the discriminant score z, 32 out of 48 tested local cultivars were classified into indica , 5 into japonica , and 11 intermediate. An analysis of ORFIOO of CtDNA by PCR identified 29 cultivars as deletion type (indica ), and 22 as non-deletion type Japonica ). Random amplified polymorphic DNA analysis revealed a high degree of polymorphism in the 51 cultivars, and 4 major clusters were recognized in a dendrogram constructed by UPGMA: 29 cultivars formed a cluster with typical indica standards

  Japanese Society of Breeding, Jun. 1996, Breeding science, 46(2) (2), 159 - 166, English

  [Refereed]


• Genetic diversity and subspecies differentiation in local rice cutlivars from manipur state of India

  Chukichi Kaneda, Masato Umikawa, M. Rohinikumar Singh, Chiharu Nakamura, Naoki Mori

  Japanese Society of Breeding, 1996, Breeding Science, 46(2) (2), 159 - 166, English

  [Refereed]

  Scientific journal


• Overexpression of mitochondrial genes in alloplasmic common wheat with a cytoplasm of wheatgrass (Agropyron trichophorum) showing depressed vigor and male sterility

  Tohru Suzuki, Chiharu Nakamura, Naoki Mori, Chukichi Kaneda

  Kluwer Academic Publishers, Feb. 1995, Plant Molecular Biology, 27(3) (3), 553 - 565, English

  [Refereed]

  Scientific journal


• Wheat phylogeny determined by RFLP analysis of nuclear DNA. 2. Wild tetraploid wheats

  N. Mori, Y. G. Liu, K. Tsunewaki

  Springer-Verlag, Jan. 1995, Theoretical and Applied Genetics, 90(1) (1), 129 - 134, English

  [Refereed]

  Scientific journal


• Restriction fragment length polymorphisms of mitochondrial DNAs from seven Fusarium species causing fusarium head blight

  Naohiko Kodo, Chiharu Nakamura, Naoki Mori, Chukichi Kaneda, Hajime Kato, Takumi Yoshizawa

  1995, Japanese Journal of Genetics, 70(3) (3), 435 - 451, English

  [Refereed]

  Scientific journal


• Physical mapping of chloroplast DNAs of Agropyron glaucum, Ag. trichophorum and Haynaldia villosa using alloplasmic common wheat with cytoplasms of respective species

  Chiharu Nakamura, Katsuki Ohtani, Naoki Mori, Chukichi Kaneda, Ivan Panayotov

  1994, Japanese Journal of Genetics, 69(6) (6), 645 - 655, English

  [Refereed]

  Scientific journal


• Molecular variation in chloroplast DNA regions in ancestral species of wheat

  N. T. Miyashita, N. Mori, K. Tsunewaki

  1994, Genetics, 137(3) (3), 883 - 889, English

  [Refereed]


• Homoeologous group 1 chromosomes of Agropyron restore nucleus-cytoplasmic compatibility in alloplasmic common wheat with Agropyron cytoplasms

  Tohru Suzuki, Chiharu Nakamura, Naoki Mori, Yoko Iwasa, Chukichi Kaneda

  1994, Japanese Journal of Genetics, 69(1) (1), 41 - 51, English

  [Refereed]

  Scientific journal


• Mycotoxin production and pathogenicity of Fusarium species and wheat resistance to fusarium head blight

  Z. Atanassov, C. Nakamura, N. Mori, C. Kaneda, H. Kato, Jin Yin Zhe, T. Yoshizawa, K. Murai

  Canadian Science Publishing, 1994, Canadian Journal of Botany, 72(2) (2), 161 - 167, English

  [Refereed]

  Scientific journal


• Comparative study on the chloroplast, mitochondrial and nuclear genome differentiation in two cultivated rice species, Oryza sativa and O. glaberrima, by RFLP analyses

  T. Ishii, T. Terachi, N. Mori, K. Tsunewaki

  Springer-Verlag, Mar. 1993, Theoretical and Applied Genetics, 86(1) (1), 88 - 96, English

  [Refereed]

  Scientific journal


• Chloroplast and mitochondrial DNAs of alloplasmic common wheat with cytoplasms of Agropyron glaucum, Ag. trichophorum and Haynaldia villosa

  SUZUKI Tohru, MORI Naoki, NAKAMURA Chiharu, PANAYOTOV Ivan, KANEDA Chukichi

  Cytoplasms of Agropyron glaucum, Ag. trichophorum and Haynaldia villosa are functionally different as revealed by several physiological characteristics of the alloplasmic lines having these cytoplasms and the same wheat nucleus. To find genetic differences among the cytoplasms of these species at molecular level, we studied chloroplast (ct) and mitochondrial (mt) DNAs using the alloplasmic lines. Cytoplasms of the two Agropyron species had closely related ctDNAs, whereas their mtDNAs differed greatly in their structure. As compared to the ct and mtDNAs of common wheat, those of the Agropyron species and Hy. villosa were found to have been equally diverged.
  

  The Genetics Society of Japan, Oct. 1992, Genes & Genetic Systems, 67(5) (5), 405 - 414, English


• Distribution of the necrosis and chlorosis genes in two wild tetraploid wheats, Triticum dicoccoides and T. araraticum

  Naoki Mori, Koichiro Tsunewaki

  1992, Japanese Journal of Genetics, 67(5) (5), 371 - 380, English

  [Refereed]

  Scientific journal


• Interaction between telocentric nucleolus-organizing chromosomes of two Agropyron species and their cytoplasms in alloplasmic lines of common wheat

  T. Suzuki, C. Nakamura, H. Sakagami, N. Mori, I. Panayotov, C. Kaneda

  1992, Japanese Journal of Genetics, 67(2) (2), 133 - 145, English

  [Refereed]

  Scientific journal


• CYTOPLASMIC AND NUCLEAR-DNA DIFFERENTIATION IN CULTIVATED RICE SPECIES

  T ISHII, T TERACHI, N MORI, K TSUNEWAKI

  1991, RICE GENETICS II, 655 - 657, English

  International conference proceedings


• Cytoplasmic diversity in alloplasmic common wheats with cytoplasms of Triticum and Aegilops revealed by photosynthetic and respiratory characteristics.

  NAKAMURA Chiharu, KASAI Kazue, KUBOTA Yoshiko, YAMAGAMI Chieko, SUZUKI Tohru, MORI Naoki

  Photosynthetic and respiratory characteristics were surveyed to evaluate cytoplasmic diversities in alloplasmic common wheats in which a nuclear genome of common wheat (Triticum aestivum L. cv. Chinese Spring (CS)) was combined with cytoplasms of Triticum and Aegilops species. The photosynthetic capacity (PSC) was measured based on the ¹³CO₂-assimilation rate by infrared absorption spectrophotometry. A significant variation was found in PSC. The alloplasmic line with a cytoplasm of T. monococcum ssp. boeoticum which is unique in causing severe growth depression and male sterility when combined with CS nuclei showed more than 30% higher PSC than the euplasmic CS. A negative correlation was found between PSC and growth vigor of the euplasmic and alloplasmic lines. Respiratory electron flows and the cytochrome c oxidase (COX) activity were measured polarographically by an oxygen electrode. The cytochrome path activity, the COX activity and the alternative path capacity showed significant variations among the lines, irrespective of plant tissues studied. The alloplasmic line with T. monococcum cytoplasm showed chracteristic respiration in the alloplasmic line: It significantly increased the alternative path capacity and the COX activity in roots and leaves of 2-week-old plants. Moreover, the COX activity in the roots was negatively correlated with growth vigor among the lines. The results suggested that the aberrantly high COX activity might be related to the growth depression of the alloplasmic CS with T. monococcum cytoplasm.
  

  The Genetics Society of Japan, 1991, Genes & Genetic Systems, 66(4) (4), 471 - 483, English


• Genetical studies on a Tibetan semi-wild wheat, Triticum aestivum ssp. tibetanum

  Koichiro Tsunewaki, Shinji Yamada, Naoki Mori

  1990, The Japanese Journal of Genetics, 65(5) (5), 353 - 365, English

  [Refereed]

  Scientific journal


• Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic DNA library construction and RFLP analysis in common wheat

  Yao Guang Liu, Koichiro Tsunewaki, Naoki Mori

  1990, The Japanese Journal of Genetics, 65(5) (5), 367 - 380, English

  [Refereed]

  Scientific journal

• NBRP-Wheat: Development of large-scale mapping populations of wheat genetic resources for future research

  太田敦士, 太田敦士, 新田みゆき, 新田みゆき, 那須田周平, 那須田周平, 吉田健太郎, 吉田健太郎, 堺俊之, 堺俊之, 竹中祥太朗, 松岡由浩, 森直樹, 西嶋遼, 寺内良平, 寺内良平

  2024, 育種学研究, 26


• Investigation of novel male sterility genes in alloplasmic lines of wheat

  辻村真衣, 宮本大輝, 竹中祥太朗, 森直樹, 寺地徹

  2024, 育種学研究, 26


• Genetic diversity of wild emmer wheat in natural populations in southern Turkey and attempts to conserve it through the development of quasi-natural populations

  MORI Naoki, SHIZUKA Takako, TAKENAKA Shotaro, TANNO Kenichi, OZKAN Hakan, OHTA Shoji

  2023, 日本進化学会大会プログラム・講演要旨集(Web), 25th


• Investigation of the male sterility observed in alloplasmic lines of wheat (cv. Chinese Spring) with Triticum-Aegilops cytoplasm. 1. Plasmon Type: U, M, T², C, G

  辻村真衣, 竹中祥太朗, 中田聖月, 森直樹, 寺地徹

  2022, 育種学研究, 24


• Genetic effect of the Aegilops caudata plasmon on manifestation of the Ae. cylindrica genome

  Koichiro Tsunewaki, Naoki Mori, Tatsuya Yotsumoto, Shigeo Takumi

  Dec. 2014, GENES & GENETIC SYSTEMS, 89(6) (6), 305 - 305, English

  Summary international conference


• Conventional and molecular studies on brown planthopper (Nilaparvata lugens Stal) resistance genes in rice: a basis for future study of natural insect resistance genes using molecular markers in Nepal

  Prem N. Sharma, Naoki Mori, Shigeo Takumi, Chiharu Nakamura

  Rice productivity greatly affected by various biotic and abiotic stresses. Insect pest are one of the major biotic constraints to cause significant losses in rice production. Brown plant hopper (BPH), Nilaparvata rugense Stal, is the most serious insect-pest of rice in Asia where most of the world rice is produced. Controlling insects using chemicals is already proven detrim

  Jul. 2014, Nepal Journal of Science and Technology, 15(1) (1), 145 - 156, English

  Introduction scientific journal


• Returning the Aegilops caudata plasmon to its home after half a century of its departure: reconstitution of Ae. caudata by reuniting its genome with the plasmon that coexisted with the common wheat genome for 50 generations

  Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

  Dec. 2011, GENES & GENETIC SYSTEMS, 86(6) (6), 411 - 411, English

  Summary international conference


• Production and characterization of Aegilops cylindrica having the plasmon of Ae. caudata

  Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

  Dec. 2009, GENES & GENETIC SYSTEMS, 84(6) (6), 465 - 465, English

  Summary international conference


• Possible horizontal transfer of Mutator-like elements within Poaceae

  Nobuaki Asakura, Naoki Mori, Shinya Yoshida, Ichiro Ohtsuka, Chiharu Nakamura

  Dec. 2007, GENES & GENETIC SYSTEMS, 82(6) (6), 526 - 526, English

  Summary international conference


• Mitochondrial microsatellite variability in the ancestral species of wheat (Aegilops speltoides Tausch.)

  Yoshiaki Ohmichi, Takashige Ishii, Chiharu Nakamura, Naoki Mori

  Dec. 2007, GENES & GENETIC SYSTEMS, 82(6) (6), 538 - 538, English

  Summary international conference


• Conversion of AFLP markers linked to the rice brown planthopper-resistant gene bph2 into PCR markers

  TORII A., TAKUMI S., MORI N., NAKAMURA C.

  30 Mar. 2002, 育種学研究 = Breeding research, 4(1) (1), 87 - 87, Japanese


• トビイロウンカ(Nilaparvata lugens Stal)抵抗性遺伝子bph4の座乗イネ染色体の決定

  川口 充洋, 村田 和優, 石井 尊生, 宅見 薫雄, 森 直樹, 中村 千春

  日本育種学会, 01 Mar. 2001, 育種学研究, 3(1) (1), 71 - 72, Japanese


• Morphological and genetic analyses of the Hooded awnless mutation in wheat.

  TAKUMI S., KOSUGI T., KOGA H., KAWAHARA T., MORI N., NAKAMURA C.

  25 Sep. 2000, 育種学研究 = Breeding research, 2(2) (2), 257 - 257, Japanese


• Mapping of a rice brown planthopper resistance gene bph2 by high efficiency AFLP marker system

  MURAI H., HASHIMOTO Z., TAKUMI S., MORI N., KAWASAKI S., NAKAMURA C.

  Apr. 2000, 育種学研究 = Breeding research, 2(1) (1), 53 - 53, Japanese


• Complete sequence of wheat chloroplast DNA. IV. MITE like sequence found in wheat chloroplast DNA

  OGIHARA Y., ISONO K., KOJIMA T., ENDO A., MURAI R., MURAI K., HANAOKA M., SHIINA T., TERACHI T., UTSUGI S., MURATA M., MORI N., TAKUMI S., IKEO K., GOJOBORI T., MATSUOKA Y., OHNISHI Y., TAJIRI H., TSUNEWAKI K.

  Apr. 2000, 育種学研究 = Breeding research, 2(1) (1), 30 - 30, Japanese


• Comparison of genomic sequences of three homoeologous loci of a knotted1-like homeobox gene, wknox1, in common wheat

  KOSUGI T., TAKUMI S., MORI N., NAKAMURA C.

  Apr. 2000, 育種学研究 = Breeding research, 2(1) (1), 111 - 111, Japanese


• Trans-activation of a maize Ds transposable element in transgenic wheat plants revealed by expression of marker genes

  TAKUMI S., IKAWA Y., MORI N., NAKAMURA C.

  Apr. 2000, 育種学研究 = Breeding research, 2(1) (1), 174 - 174, Japanese


• Pathogenicity, Mating Ability and DNA Restriction Fragment Length Polymorphisms of Pyricularia Populations Isolated from Gramineae, Bambusideae and Zingiberaceae Plants :

  KATO Hajime, YAMAMOTO Masaaki, YAMAGUCHI-OZAKI Tomona, KADOUCHI Hiroyuki, IWAMOTO Yutaka, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki, MORI Naoki

  Eighty-five Pyricularia isolates were collected from 29 host species of Gramineae, Bambusideae and Zingiberaceae plants sampled in Brazil, Uganda, Ivory Coast, India, Nepal, China, Indonesia and Japan.These isolates were compared on the basis of pathogenicity, mating ability and restriction fragment length polymorphisms with single-copy DNA probes.Based on the pathogenicity to eight differential gramineous plants, these isolates were classified into seven pathotypes:finger millet type, foxtail millet type, common millet type, rice type, crabgrass type, Italian ryegrass / weeping lovegrass type, and non-cereal / grass type.Genetic variation among these isolates was assessed by RFLP analysis with two restriction enzymes and nine single-copy DNA probes isolated from a finger millet strain.An UPGMA dendrogram based on the RFLPs revealed that the 85 isolates could be classified into seven major groups.Isolates from cereal crops(finger millet, foxtail millet, common millet, wheat and rice)and a grass, Brachiaria plantaginea, were clustered into a single group.They were further divided into six subgroups corresponding to the pathotypes.Among cereal crop isolates only an isolate from pearl millet was located into a different group.The remaining isolates were clustered into five groups designated as the crabgrass group, the buffelgrass and jungle rice group, the rice cutgrass, knotroot bristlegrass and Setaria tomentosa group, the bamboo and bamboo grass group and the Zingiber mioga group.The isolates from cereal crops were generally capable of mating with finger millet strains and constituted a closed mating compatibility group.These results suggested that the isolates from cereal crops from a single group with a common ancestor although they are pathogenic to taxonomically diverse plants.A combined analysis of the pathogenicity and genetic similarity suggested that the transmission of M.grisea isolates occurs in natural agroecosystems between finger millet and Eleusine africana, goosegrass or Bambusa arundinacea, between foxtail millet and green bristlegrass, and between rice and tail fescue, Italian ryegrass, sweet vernalgrass, reed canarygrass or Oryza longistaminata.

  Last, Phytopathological Society of Japan, 2000, Journal of general plant pathology : JGPP, 66(1) (1), 30 - 47, English

  [Refereed]


• CLONING AND CHARACTERIZATION OF A COLD-RESPONSIVE Wrab FAMILY IN WHEAT :

  TSUDA Kanako, TSVETANOV Sergei, OHNO Ryoko, TAKUMI Shigeo, MORI Naoki, NAKAMURA Chiharu

  Japanese Society of Plant Physiologists, 2000, Plant and cell physiology, 41, s49, English


• CLONING AND CHARACTERIZATION OF A COLD-RESPONSIVE GENE WCOR14 IN WHEAT :

  OHNO Ryoko, TSVETANOV Sergei, TSUDA Kanako, TAKUMI Shigeo, MORI Naoki, ATANASSOV Atanas, NAKAMURA Chiharu

  Japanese Society of Plant Physiologists, 2000, Plant and cell physiology, 41, s49, English


• Trans-activation of a maize Ds transposable element in transgenic wheat plants expressing the Ac transposase gene

  S Takumi, K Murai, N Mori, C Nakamura

  May 1999, THEORETICAL AND APPLIED GENETICS, 98(6-7) (6-7), 947 - 953, English

  [Refereed]


• CLONING AND CHARACTERIZATION OF G PROTEIN α SUBUNIT cDNAs FROM TABACCO HAIRY ROOTS

  ANDO Seiji, TAKUMI Shigeo, MORI Naoki, NAKAMURA Chiharu

  Mar. 1999, Plant and cell physiology, 40, s12 - s12, English


• Complete sequence of wheat chloroplast DNA.

  荻原保成, 磯野一宏, 村井里佳, 華岡光正, 寺地徹, 宇都木繁子, 森直樹, 池尾一穂, 松岡由浩

  1999, 育種学研究, 1


• Complete sequence of wheat chloroplast DNA

  OGIHARA Yasunari, ISONO Kazuhiro, KOJIMA Toshio, ENDO Akira, MURAI Rika, MURAI Koji, HANAOKA Mitsumasa, SHIINA Takashi, TERACHI Toru, UTSUGI Shigeko, MURATA Minoru, MORI Naoki, TAKUMI Shigeo, IKEO Kazuho, GOJOBORI Takashi, MATSUOKA Yoshihiro, OHNISHI Yukari, TAJIRI Hikaru, TSUNEWAKI Koichiro

  01 Dec. 1998, 日本分子生物学会年会プログラム・講演要旨集, 21, 481 - 481, Japanese


• コムギ葉緑体DNAの全塩基配列決定

  荻原保成, 磯野一宏, 村井里佳, 華岡光正, 寺地徹, 宇都木繁子, 森直樹, 池尾一穂, 常脇恒一郎

  1998, 日本分子生物学会年会プログラム・講演要旨集, 21st


• Decision of all structures of wheat chloroplast genome II. Entire base arrangement of wheat chloroplast DNA.

  荻原保成, 磯野一宏, 村井里佳, 華岡光正, 寺地徹, 宇都木繁子, 森直樹, 池尾一穂, 常脇恒一郎

  1998, 日本遺伝学会大会プログラム・予稿集, 70th


• E310 Selection of virulent populations of brown planthopper (BPH) on rice varieties with BPH resistance genes, bph8 and Bph9

  Yasaratna Ketipearachchi, Nakamura Chiharu, Kaneda Chukichi, Mori Naoki

  Japanese Society of Applied Entomology and Zoology, 01 Apr. 1997, 日本応用動物昆虫学会大会講演要旨, (41) (41), 147 - 147, English


• Molecular Mapping of the Gene for Brown Planthopper Resistance in Rice

  Murata Kazumasa, Kaneda Chukichi, Nakamura Chiharu, Mori Naoki

  Kobe University, 01 Mar. 1996, Memoirs of the Graduate School of Science and Technology, Kobe University. A, 14, 35 - 43, English


• Parasitic Specialization of Magnaporthe grisea : 9. RFLP Analysis of Genomic DNA

  YAMAMOTO M., NAKAYASHIKI H., YAMAGUCHI T., MORI N., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 231 - 231, Japanese


• Parasitic Specialization of Magnaporthe grisea 8. : RFLP Analysis of Genomic DNA (2)

  MORI N., YAMAMOTO M., NAKAYASHIKI H., OZAKI T., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1994, Annals of the Phytopathological Society of Japan, 60(3) (3), 346 - 347, Japanese


• Parasitic Specialization on Magnaporthe grisea. : 7. RFLP Analysis of Genomic DNA (1)

  MORI N., OZAKI T., IWAMOTO Y., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1993, Annals of the Phytopathological Society of Japan, 59(3) (3), 270 - 270, Japanese


• Geographical distribution of T. dicoccoides strains having different morphological and physiological and characters.

  MORI N., TSUNEWAKI K.

  Japanese Society of Breeding, 1986, Japanese Journal of Breeding, 36(2) (2), 20 - 21, Japanese

• Essential Genetics and Genomics, 7th Edition

  Hartl, Daniel L, 中村, 千春, 岡田, 清孝, 森,直樹

  Joint translation, 第14章, 化学同人, Jan. 2021, Japanese, ISBN: 9784759820485


• インダス 南アジア基層世界を探る（長田俊樹 編）, 第11章 インド冬作穀類の起源と変遷

  Ohta Shoji, MORI NAOKI

  Others, 京都大学学術出版会, Oct. 2013, Japanese, 紀元前2600年頃からインド亜大陸北西部で栄えたインダス文明は、紀元前1900年頃に衰退したことが知られている。しかし、なぜ衰退したのかについてはいまだに不明である。地球環境学研究所では、当時のインダス地域における人類社会と自然環境を復元することにより、その衰退原因を明らかにする研究（プロジェクト代表者：長田俊樹）が行われた。筆者らは、このプロジェクトの一員として現代インドに残る伝統的な農業体系とその中に数千年にわたって継続的に栽培されてきた在来コムギについて現地調査を行い、当時の農耕システムの復元を試みたので、その成果を第11章に紹介した。, ISBN: 9784876983001

  Scholarly book


• インダス 南アジア基層世界を探る（長田俊樹 編）, コラム 「それなら知っているよ Gund godiだよ。」ーインド矮性コムギ再発見の日ー

  MORI NAOKI

  Others, 京都大学学術出版会, Oct. 2013, Japanese, ISBN: 9784876983001

  Scholarly book


• Natural history of wheat

  MORI NAOKI

  Joint work, 北海道大学出版会, Mar. 2010, Japanese, 人が麦を栽培し始めてから現在までの人と麦の関係をテーマに、麦類の由来、歴史、細胞遺伝学、進化学、考古学、民族植物学、文化人類学といった幅広い視点から栽培植物としての麦類について記されたものである。筆者は第3章「染色体数の倍加により進化したコムギ」を担当, ISBN: 9784832981904

  Scholarly book

• Evaluation of genetic diversity and the development of quasi-natural populations of wild emmer wheat populations in southern Turkey

  Naoki Mori, Shotaro Takenaka, Kenichi Tanno, Hakan Ozkan, Shoji, Ohta

  日本育種学会第１４７回講演会, Mar. 2025


• Comparison of genetic diversity between chondriome and plastome in wild tetraploid wheat

  H. Akiyama, T. Shizuka, T. Ishii, N. Mori

  第１９回麦類研究会（東京農業大学）, Dec. 2024


• Evaluation of the domestication traits of spike in emmer wheat using recombinant inbred lines (RILs)

  T. Yamamichi, N. Mori

  第19回ムギ類研究会参（東京農業大学）, Dec. 2024


• Mapping of loci controlling the trichome on wheat glume in emmer wheat

  G.Tokuyama, K.Nishimura, N.Mori

  第１９回ムギ類研究会（東京農業大学）, Dec. 2024


• Genetic study of QTLs controlling grain size and seed dormancy in wild emmer wheat

  A. Nakamura, Y. Sakai, N. Mori, K. Nishimura, S. Ohta

  第１９回ムギ類研究会（東京農業大学）, Dec. 2024


• Identification of genomic region of tetraploid wheat involved in unreduced gamete formation by QTL-seq

  D. Nomura, Y. Matsuoka, N. Mori, K. Yoshida

  日本育種学会 第１４６回講演会, Sep. 2024


• An alysis of genetic di ff erentia tion a nd he a ding d a te diversity in wild emmer whe a t collected from Isra el

  張 芸瑄, 西村, 和紗, 角坂, 麻衣, 村田, 和樹, 田村, 朋子, 岩橋, 優, 元木, 航, 長坂, 京香, 牧, 隆宏, 木下, 有羽, 中野, 龍平, 井上, 博茂, 門田, 有希, 川浦, 香奈子, 森, 直樹, 加藤, 鎌司, 西田, 英隆, 中﨑 鉄也

  Sep. 2024


• Investigation of novel male sterility genes in alloplasmic lines of wheat.

  Tsujimura, M., H. Miyamoto, S. Takenaka, N. Mori, T. Terachi

  日本育種学会第１４５回講演会, Mar. 2024


• Genetic diversity of wild emmer wheat in natural populations in southern Turkey and attempts to conserve it through the development of quasi-natural populations

  Naoki Mori, Takako Shizuka, Shotaro Takenaka, Kenichi Tanno, Hakan Ozkan, Shoji Ohta, Grad. Sch, Agric. Sci, Kobe Univ, Fac. Agric, Ryukoku Univ, Fac. Litl, Ryukoku Univ, Fac. Agric, Univ. Cukurova, Turkey, Prof. emeritus, Fukui Pref

  日本進化学会 第25回沖縄大会, Aug. 2023, English

  Oral presentation


• Quantitative analysis of intra-molecular recombination in mitochondrial genome in alloplasmic wheat

  U. Nakagawa, T. Kamada, S. Ohta, M. Makita, M. Tsujimura, Terachi, N. Mori

  第17回ムギ類研究会, Dec. 2022, Japanese

  Poster presentation


• Intraspecific variation of Aegilops geniculata revealed by chloroplast SSR analysis

  Lee BongAn, Naoki Mori, Shoji Ohta

  第17回 ムギ類研究会, Dec. 2022, Japanese

  Poster presentation


• Genetic analysis of cultivation-associated traits of ear and grass type using backcross derived lines in emmer wheat.

  M. Morioka, S. Kasubuchi, S. Shimada, K. Gyu, C. Vladutu, S. Kiania, N. Mori

  第17回ムギ類研究会, Dec. 2022, Japanese

  Poster presentation


• Investigation of the male sterility observed in alloplasmic lines of common wheat (cv. Chinese Spring) with Triticum-Aegilops cytoplasm. 1. Plasmon Type: U, M, T2, C, G

  Tsujimura, M, S. Takenaka, M. Nakata, N. Mori, T. Terachi

  日本育種学会 第142回講演会, Sep. 2022, Japanese

  Poster presentation


• Genetic analysis of grain morphoLogy in wild and domesticated emmer wheat

  S. Iwasaki, N. Mori

  第16回 ムギ類研究会, Dec. 2021, Japanese

  Poster presentation


• 野生エンマーコムギにおけるVRN-A3座の変異と栽培コムギにおける分布

  西村 和紗, 森 直樹, 半田 裕一, 川浦 香奈子, 北島 宣, 中﨑 鉄也

  日本育種学会第139回講演会, Mar. 2021, Japanese

  Poster presentation


• コムギ倍数性進化における雑種ゲノム倍加の重要性:交雑実験とQTL解析の結果から

  松岡 由浩, 森 直樹

  第15回ムギ類研究会, Dec. 2020, Japanese

  Poster presentation


• Comparative analysis of spike and grain morphology in wild and domesticated emmer wheat

  S.Iwasaki, N. Mori

  第15回ムギ類研究会, Dec. 2020, Japanese

  Poster presentation


• Genetic analysis of the plant architecture using backcross derived lines in emmer wheat.

  S.Kasubuchi, S. Shimada, K. Gyu, C. Vladutu, S. Kianian, N. Mori

  第15回ムギ類研究会, Dec. 2020, Japanese

  Poster presentation


• Quantitative analysis of intra-molecular recombination of mitochondrial genome in alloplasmic wheat

  T. Kamada, S. Ohta, M. Makita, M. Tsujimura, T. Terachi, N. Mori

  第15回ムギ類研究会, Dec. 2020, Japanese

  Poster presentation


• Role of hybrid genome doubling in polyploid wheat evolution

  Matsuoka Yoshihiro, Mori Naoki

  22nd Annual Meeting of the Society of Evolutionary Studies, Japan, Sep. 2020, Japanese

  Poster presentation


• Study on the reciprocal recombination across short repeated sequences dispersed throughout the wheat mitochondrial genome

  Tsujimura, M., M. Sato, I. Nagashima, N. Mori, T. Terachi

  Japanese Society of Plant Breeding, Mar. 2020, Japanese

  Oral presentation


• Genetic analysis of grain dimensions and dormancy using F3 populations in emmer wheat

  T. Fukuyama, N. Mori, S. Ohta

  第14回ムギ類研究会, Nov. 2019

  Poster presentation


• Genetic analysis of morphological traits of spike using backcross recombinant inbred lines derived from a cross between wild and domesticated emmer wheat

  Y . Yamaguchi, Y. Miyazaki, N. Mori

  第14回ムギ類研究会, Nov. 2019

  Poster presentation


• Quantitative analysis of intra-molecular recombination of mitochondrial genome in alloplasmic wheat

  T. Kamada, S. Ohta, M. Makita, M. Tsujimura, T. Terachi, N. Mori

  第14回ムギ類研究会, Nov. 2019

  Poster presentation


• Genetic analysis of seed dormancy and grain number per spikelet in Einkorn wheat

  N. Matsui, N. Mori, S. Ohta

  第14回ムギ類研究会, Nov. 2019

  Poster presentation


• Genetic analysis of the plant architecture using backcross derived lines in emmer wheat

  S. Kasubuchi, S. Shimada, K. Gyu, C. Vladutu, S. Kianian, N. Mori

  第14回ムギ類研究会, Nov. 2019

  Poster presentation


• Intraspecific variation and genetic differentiation of Aegilops geniculata

  Y. Yoshimura, N. Mori, S. Ohta

  第14回ムギ類研究会, Nov. 2019

  Poster presentation


• Natural variation for crossability with Aegilops tauschii Coss. and seedset rates of F1 hybrids with Ae. tauschii in Triticum turgidum L.

  Y. Matsuoka, N. Mori

  第14回ムギ類研究科, Nov. 2019

  Poster presentation


• Frequency diversity of non-dormant individuals among wild diploid wheat populations in southern Turkey

  Ohta Shoji, Mori Naoki, Ozkan Hakan

  日本育種学会第135回講演会, Mar. 2019, Japanese, 日本育種学会, 千葉大学 西千葉キャンパス, 演者らはこれまで野生二倍性コムギの1種である野生一粒系コムギの種子休眠性について、①休眠性の個体が同一小穂の第1小花に小さくて休眠性、第2小花に大きくて非休眠性の穎果を着ける、②第2小花に比べて第1小花の穎果が小さい個体ほど強い休眠性をもつ、③小穂内に単独で着いた穎果は着粒した小花に係らず大きくて非休眠性である、④野生型の中に栽培型と同じように非休眠性で第1小花に大きな穎果を着ける個体があること、を明らかにしてきた。本研究では、野生二倍性コムギ集団の発芽適応戦略と栽培コムギ集団の成立過程を明らかにする目的で、栽培化起源地の野生二倍性コムギ集団における非休眠性個体の頻度を調査した。 【材料と方法】トルコ南部の野生二倍性コムギ集団で1穂/個体を採集しチュクロワ大学で保存しているサンプルから実験集団を育成するために、11集団について1粒/穂を抽出し、201, Domestic conference

  Oral presentation


• Genetic analysis of seed dormancy and grain number per spikelet in Einkorn wheat

  Nene Matsui, Naoki Mori, Shoji Ohta

  第13回 ムギ類研究会, Nov. 2018, Japanese, ムギ類研究会, 横浜市立大学 木原生物学研究所, 一粒系コムギには、小穂あたり種子が1粒着粒する傾向が高いものと2粒着粒する傾向が高いものがあり、系統間で変異がみられる。小穂あたり2粒着粒する野生一粒系コムギでは、同一小穂内の2つの穎果のうち、第1小花に着粒した穎果は休眠性をもち、またサイズが小さい。この形質は栽培型では消失している。本研究では、これらの形質についてその遺伝様式を知るためにQTL解析を行っている。, Domestic conference

  Poster presentation


• Distribution of wild emmer and wild timopheevi wheat within and among wild tetraploid wheat populations in southern Turkey

  Shoji Ohta, Naoki Mori, Hakan Ozkan

  第13回ムギ類研究会, Nov. 2018, Japanese, 横浜市立大学 木原生物学研究所, 四倍性コムギには二粒系コムギとチモフェービ系コムギが含まれる。両種が自生するトルコ南部で収集した集団サンプルを用いて集団内・集団間の多様性を分析しているが、両種の野生型亜種の穂の形態は酷似し、穂のサンプルから両者を区別することは困難である。今回、チュクロワ大学で比較栽培した11集団442個体について葉面の毛の形質により両種を同定し集団レベルでの分布を明らかにした。本研究はJSPS科研費 JP16K07559, JP17K07272の助成を受けている。, Domestic conference

  Poster presentation


• Intraspecific variation and gnentic differentiation of Aegilops geniculata

  吉村優介, MORI NAOKI, 大田正次

  第13回ムギ類研究会, Nov. 2018, Japanese, 横浜市立大学 木原生物学研究所, コムギの近縁野生種Aegilops geniculata Rothは、種内に大きな形態的変異がみられ、細胞遺伝学的および遺伝学的にも大きな変異が含まれている。本研究では、まず、葉緑体ゲノムのマイクロサテライト座の多型を調査し、種内の系統関係や遺伝的分化の様相を明らかにする。これまでの解析結果から、亜種内、変種内にも大きな変異が存在することが判明した。また、出穂・開花時期などの生理的形質にも種内変異がみられた。, Domestic conference

  Poster presentation


• Genetic analysis of grain dimensions and dormancy using F3 populations in emmer wheat

  Tomoaki Fukuyama, Naoki Mori, Shoji Ohta

  第13回 ムギ類研究会, Nov. 2018, Japanese, 横浜市立大学 木原生物学研究所, 野生エンマ―コムギでは多くの場合、第1小花に着粒する頴果の休眠性が深く、また、第2小花の頴果と比べ、第1小花の頴果は小さくて軽い。本研究では、これらの形質の生理学的な調査と関与するQTLの位置の推定を行っている。発芽試験の結果、野生エンマ―コムギの第1小花の頴果の約50％は約半年で休眠性を失うことがわかった。また、4B染色体に頴果の大きさの比に関するQTLが存在する可能性が示唆された。, Domestic conference

  Poster presentation


• Genetic analysis of the ratio of grain weight to spikelet weight using backcross recombinant inbred lines direived from a cross between wild and domesticated emmer wheat

  Yuhei Yamaguchi, Yuki Miyazaki, Naoki Mori

  第13回 ムギ類研究会, Nov. 2018, Japanese, ムギ類研究会, 横浜市立大学 木原生物学研究所, 〇・・森直樹（神戸大・農学研究科） ◦, N. Mori (Grad. Sch. Agr. Sci., Kobe U.) これまでに、野生エンマーコムギ (Triticum turgidum ssp. dicoccoides) と栽培エンマーコムギ (Triticum turgidum ssp. dicoccum) を用いて育成された92の戻し交雑自殖系統を用い、野生種と栽培種の間で大きく異なる性質について２反復で栽培して継続的に調査してきた。また、この戻し交雑自殖系統についてSSRマーカーの遺伝子型を決定し、栽培化関連QTLの位置や効果を解析してきた。今回は小穂に占める穎果の割合と穎果の形態に関する形質について主に調査したので報告する。, Domestic conference

  Poster presentation


• Diversity in the frequency of non-dormant individuals among wild tetraploid wheat population in southern Turkey

  Ohta Shoji, MORI NAOKI, Ozkan Hakan

  一般社団法人日本育種学会 第133回講演会, Mar. 2018, Japanese, Kyusyu University, トルコ南部の栽培化起源地域の11集団で収集し、チュクロワ大学で保存している穂のサンプルから1穂あたり1粒を抽出した約400個体の野生四倍性コムギの実験集団を、2014年～2015年と2016年～2017年にチュクロワ大学で比較栽培し、それぞれ2015年秋と2017年秋に種子休眠性の程度とそれに相関した形態形質の変異を調査した。その結果、自然集団にはさまざまな頻度で非休眠性の個体が含まれていることが明らかとなり、また、生育環境の良否によって集団中の非休眠性種子の頻度が変動することが示唆された。これらの結果から、野生四倍性コムギ集団の自然環境下における発芽適応戦略と栽培コムギ集団の成立過程について考察した。, Domestic conference

  Oral presentation


• Effect of the novel Vrn-A3 allele in a spelt wheat (Triticum aestivum L. ssp. spelta) accession, KT19-1 on earliness of wheat

  Nishimura, K, Saito Takanari, Handa Yuichi, Mori Naoki, Kawaura Kanako, Kitajima, A, Nakazaki Tetsuya

  一般社団法人日本育種学会 第133回講演会, Mar. 2018, Japanese, Kyusyu University, 【緒言】本邦のコムギ栽培において、登熟期が梅雨の時期と重なり穂発芽などの雨害が発生し、収量および品質の低下が問題となっているため、早生化が重要な育種目標である。これまでの研究で、四倍体コムギ系統TN26(T. turgidum L. ssp. dicoccum)がプロモーター領域に特異的な挿入配列を持つ新規Vrn-A3早生アレル(挿入型Vrn-A3)を保有することを見出した。一方、多様な六倍体コムギ系統を用いて、挿入型Vrn-A3の分布を調査したところ、ドイツ原産のスペルトコムギ系統(T. aestivum L. ssp. spelta)がTN26と同様の挿入型アレルを保有することが明らかとなった。本研究では、挿入型Vrn-A3を保有するスペルトコムギ系統KT19-1と普通コムギ系統CSとの交雑RILsを用いて、KT19-1が保有するVrn-A3挿入, Domestic conference

  Poster presentation


• Distribution of the early flowering allele of Vrn-A3 of the tetraploid wheat line TN26 in hexaploid wheat lines (Triticum aestivum L.)

  Nishimura, K, Saito Takanari, Mori Naoki, Handa Yuichi, Kitajima, A, Nakazaki Tetsuya

  第12回ムギ類研究会, Dec. 2017, Japanese, Kyoto University, 本研究では、四倍体コムギ系統TN26 (T. turgidum L. ssp. dicoccum) が保有するVrn-A3早生アレルの六倍体コムギにおける分布を解析した。日本品種のミニコアコレクション (Kobayashi et al. 2016) であるJWC96系統を用いた解析の結果、早生アレルを保有する系統は北海道や東北における15品種に見られた。次に、Hirosawa et al. (2004) の供試材料85系統を用いた解析の結果、ドイツ原産のスペルトコムギ系統が早生アレルを保有することが明らかとなった。これらの結果から、このアレルの活用が六倍体品種の早生化育種に有効である可能性を見出した。, Domestic conference

  Poster presentation


• Intraspecific variation and geographical differentiation of wild emmer wheat revealed by morphological and genetic analyses

  Murakawa Natsuki, Mori Naoki, Özkan Hakan, Ohta Shoji

  第12回ムギ類研究会, Dec. 2017, Japanese, Kyoto University, 自然分布域をほぼカバーするように採集された57系統の野生二粒系コムギを使用して、形質調査とSSRマーカーによるミトコンドリアDNAの解析を行った。調査の結果、形態的形質について本種は種内に大きな多様性を持っており、いくつかの形質については採集された地域によって大きな偏りがあることがわかった。また、現在解析中のミトコンドリアゲノムには､これまでのところほとんど変異が見られないことがわかった。, Domestic conference

  Poster presentation


• Genetic analysis of the domestication related traits using backcross derived lines in emmer wheat.

  Shimada Saori, Gyu Koichi, Vladutu Cristian, Kianian Shahryar, MORI NAOKI

  第12回ムギ類研究会, Dec. 2017, Japanese, 京都大学農学部, エンマーコムギは最も初期に栽培化されたコムギといわれている。本研究では、エンマーコムギの栽培化の初期に起こった遺伝的変化の解明を目的として、野生エンマーコムギと栽培エンマーコムギから作出された野生種の遺伝的背景を持つ戻し交雑自殖系統について、それらの栽培化関連形質を調査した。また、分子マーカーによるQTL解析を行うことで、エンマーコムギの栽培化関連形質を支配するQTLの染色体上の位置や効果を推定した。, Domestic conference

  Poster presentation


• Toward the QTL analysis of grain dimensions and dormancy in wild emmer wheat.

  Terada Nagisai, Mori Naoki, Ohta Shoji

  第12回ムギ類研究会, Dec. 2017, Japanese, Kyoto University, 野生エンマーコムギの小穂では、第一小花と第二小花に着粒する穎果の大きさや休眠性に種内変異があり、第一小花に着粒する穎果が第二小花に比べて小さい場合、その穎果の休眠性が深くなる傾向がある。栽培エンマーコムギではこの傾向がほとんどない。本研究では、この穎果サイズや休眠性の小穂内二型性に関与するQTLを同定するため、野生エンマーコムギと栽培エンマーコムギのF2及びF3系統を用いて連鎖地図の作成を行っている。, Domestic conference

  Poster presentation


• Quantitative analysis of intra-molecular recombination of mitochondrial genome in wheat

  Ohta Seishi, Makita Masayuki, Tsujimura Mai, Terachi Toru, Mori Naoki

  第12回ムギ類研究会, Dec. 2017, Japanese, Kyoto University, ミトコンドリアはほとんど全ての真核生物に見られる細胞小器官であり、エネギー生産という重要な役割を担っている。コムギのミトコンドリアは独立した環状のDNA分子をもち、さらにこの分子内の組換えで生じた小環状分子の存在が報告されている。この小環状分子の動態はミトコンドリアの機能と関わりを持つ可能性が有るため、本研究ではコムギにおけるミトコンドリアゲノムの分子内組換え頻度の定量的解析系の確立を試みている。, Domestic conference

  Poster presentation


• Genetic diversity between and within two natural populations of wild emmer wheat (Triticum turgidum subsp. dicoccoides) in southern Turkey: insights from chloroplast DNA fingerprinting

  Naoki Mori, Takako Shizuka, Hakan Ozkan, Shoji Ohta

  8th International Triticeae Symposium 2017, Jun. 2017, English, Wernigerode/Gatersleben, Germany, Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the ancestral species of cultivated tetraploid wheat with AABB genomes (i.e. emmer wheat: T. turgidum ssp. dicoccum, durum wheat: T. turgidum ssp. turgidum conv. durum and etc.). Wild emmer wheat is an annual, predominantly self-pollinated species. Because of its full interfertility with domesticated emmer wheat, this w, International conference

  Poster presentation


• Genetic diversity between and within natural populations of wild emmer wheat (Triticum turgidum subsp. dicoccoides) in southern Turkey: Insights from nuclear-SSR and morphological variation

  Esra Çakır, Ahmad Alsaleh, Naoki Mori, Shoji Ohta, Hakan Ozkan

  8th International Triticeae Symposium 2017, Jun. 2017, English, Wernigerode/Gatersleben, Germany, Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the ancestral species of cultivated tetraploid wheat with AABB genomes. Because of its full interfertility with domesticated emmer wheat, this wild species can serve as one of the most important genetic resources to improve durum as well as bread wheat. To clarify the magnitude of genetic diversity between and within pop, International conference

  Poster presentation


• A Japan-Turkey cooperative research project on genetic diversity in wild wheat and their genetic change during domestication process

  Shoji Ohta, Naoki Mori, Hakan Ozkan

  8th International Triticeae Symposium 2017, Jun. 2017, English, Wernigerode/Gatersleben, Germany, Southeastern Turkey is located at the heart of the Fertile Crescent in Southwest Asia. It is one of the centers of genetic diversity in wild wheat, wild barley and wild relatives of Mediterranean-Southwest Asian legumes, such as lentils, chick peas, peas, and so on, and is also one of the probable candidates where einkorn and emmer wheat were domesticated from their wild proge, International conference

  Poster presentation


• Toward the QTL analysis of grain dimensions and dormancy in wild emmer wheat.

  Terada Nagisai, Ohta Shoji, MORI NAOKI

  Annual meeting of the Japanese Society of Plant Breeding, Mar. 2017, Japanese, 名古屋大学, 【目的】 種子休眠性の喪失は種子作物の栽培化に伴って起こった顕著な遺伝的変化の一つである。演者らはこれまでに野生エンマーコムギの自然集団を調査し，同一小穂の第1小花に着粒する穎果は休眠性で小さく，第2小花の穎果は非休眠性で大きい傾向があること，また休眠性の程度と小穂内の穎果サイズの違いには相関があり第2小花に比べて第1小花の穎果が小さい個体ほど休眠が深いこと，さらに小穂内にみられるこのような二型性の程度には大きな種内変異があることを明らかにしてきた（大田，第129回講演会）。本研究の目的は，野生型にみられる種子休眠性の特性を理解し，当該形質に関与する量的形質遺伝子の単離に向けて，このQTLを連鎖地図上に位置付けることである。これらの遺伝子が単離できれば，不明な点が多い野生種の環境適応戦略についても理解が深まると考える。 【材料】 野生エンマーコム, Domestic conference

  Poster presentation


• Genetic analysis of domestication related traits in emmer wheat: energy partitioning in spike and spikelet

  Miyazaki Yuki, Pham-Minh Ngoc, Liberatore Katie, Kianian Shahryar, Vladutu Cristian, MORI NAOKI

  Annual meeting of the Japanese Society of Plant Breeding, Mar. 2017, Japanese, 名古屋大学, 【目的】コムギは西南アジアにおいて倍数性進化と栽培化によって起源したと考えられている。約1万年前、人間は野生エンマーコムギ (Triticum turgidum ssp. dicoccoides)を利用し始めたが、その過程で栽培化が起こり、栽培エンマーコムギ (T. turgidum ssp. dicoccum)が生まれたとされている。エンマーコムギの野生種と栽培種の間では様々な形質で違いが見られるが、それらの遺伝的制御機構の全貌については不明な点が多い。本研究では、栽培化によって小穂重あたりの穎果重の割合が変化したのではないかという仮説を立て、このようなエネルギー配分の変化を遺伝学的に検証することを目的する。 【材料と方法】栽培エンマーコムギ (以下Dcm1001とする) を母本として野生エンマーコムギ (以下Dcc63とする) と交雑して得たF1, Domestic conference

  Poster presentation


• 酒米のルーツをさぐる - DNAでさぐる日本酒米の起源

  MORI NAOKI

  橿原市昆虫館サイエンスカフェ, Mar. 2017, Japanese, 橿原市昆虫館, 日本酒の製造に好適な一群のイネ品種群としてしられる酒造好適米（酒米）の起源について、DNAの解析結果から推定されることについて講演した。, Domestic conference

  [Invited]

  Invited oral presentation


• Genetic analysis of the domestication related traits using backcross derived lines in emmer wheat.

  Shimada Saori, Gyu Koichi, Vladutu Cristian, Ishii Takashige, Kianian Shahryar, MORI NAOKI

  Annual meeting of the Japanese Society of Plant Breeding, Mar. 2017, Japanese, 名古屋大学, ［目的] エンマーコムギ (Triticum turgidum ssp. dicoccum) は、コムギ属の中で最も初期に栽培化されて起源した栽培種の一つであり、初期農耕の起源と世界各地への伝播において重要な役割を果たした。本研究では、コムギ栽培化の初期に起こった遺伝的変化を解明することを目的として、祖先野生種 (T. turgidum ssp. dicoccoides) の遺伝的背景を持つ戻し交雑自殖系統を育成し、栽培化関連形質を評価することによりこれらを支配するQTLの解析を進めている。 ［方法］ 栽培エンマーコムギ (以下Dcm1001と呼ぶ)と野生エンマーコムギ (以下Dcc63) の交雑から野生種の遺伝的背景を持つ戻し交雑自殖系統 (BC2F14) を育成した（図１）。これらの栽培化関連形質を評価し、分子マーカーによるQTL解析により、, Domestic conference

  Poster presentation


• Quantitative analysis of intra-molecular recombination of mitochondrial genome in wheat using qPCR

  Ohta Seishi, Makita Masayuki, Tsujimura Mai, Terachi Toru, MORI NAOKI

  Annual meeting of the Japanese Society of Plant Breeding, Mar. 2017, Japanese, 名古屋大学, 【背景と目的】ミトコンドリアはほとんど全ての真核細胞に含まれる細胞小器官である。好気呼吸によるエネルギー生産という真核生物が生存する為に必要不可欠な役割を担っている。また, 核とは別に独自のゲノムを持っており, コムギを含む高等植物では不均一なサイズの小型環状DNA分子が複数存在する事が報告されている。このDNA分子の発生にはミトコンドリアゲノムにおける分子内組換えが関与しているとされているが, その詳細は不明な点が多い。 筆者らは，コムギにおいてミトコンドリアゲノムの動態と機能発現の関係を明らかにするため研究を進めている。その基盤として，ミトコンドリアゲノムの分子内組換えを正確に検出し，その頻度を定量する実験系の確立を目指している。これによって，ミトコンドリアゲノムの動態が成長段階や組織によって異なるのかどうかを調査できるものと考えている。 【方, Domestic conference

  Poster presentation


• Toward the QTL analysis of grain dimensions and dormancy in wild emmer wheat (Triticum turgidum ssp. dicoccoides)

  Terada Nagisai, MORI NAOKI, Ohta Shoji

  第11回ムギ類研究会, Dec. 2016, Japanese, 岡山大学 資源植物学研究所, 野生二粒系コムギの小穂では、第一小花と第二小花に着粒する穎果の大きさや休眠性に種内変異があり、第一小花に着粒する穎果が第二小花に比べて小さい場合、その穎果の休眠性が深くなる傾向がある。栽培二粒系コムギではこのような現象がみられない。本研究では、穎果サイズや休眠性の二形性に関与するQTLを解析するため、野生二粒系コムギと栽培二粒系コムギのF1植物に由来するF2及びF3系統を用いて連鎖地図の作成を行っている。, Domestic conference

  Poster presentation


• Genetic analysis of the domestication related traits using backcross derived lines in emmer wheat

  Shimada Saori, Gyu Koichi, Vladutu Cristian, Ishii Takashige, Kianian Shahryar, MORI NAOKI

  第11回ムギ類研究会, Dec. 2016, Japanese, 岡山大学 資源植物学研究所, エンマーコムギはコムギ属の中で最も初期に栽培化されたコムギの一つとされている。本研究では、エンマーコムギの栽培化の初期に起こった遺伝的変化の解明を目的として、野生エンマーコムギと栽培エンマーコムギから作出された野生種の遺伝的背景を持つ戻し交雑自殖系統について、それらの栽培化関連形質を調査した。また、分子マーカーによるQTL解析を行うことで、エンマーコムギの栽培化関連形質を支配するQTLの染色体上の位置や効果を推定した。, Domestic conference

  Poster presentation


• Genetic analysis of domestication related traits in emmer wheat: seed morphology and weight

  Miyaaki Yuki, Pham Minh Ngoc, Liberatore Katie, Kianian Shahryar, MORI NAOKI

  第11回ムギ類研究会, Dec. 2016, Japanese, 岡山大学 資源植物学研究所, 栽培二粒系コムギと野生二粒系コムギを親として育成した戻し交雑自殖系統92系統を用いて、穎果の大きさや形について形質調査を行った。また、210のSSRマーカーと9321のSNPマーカーの遺伝子型に基づいて分散分析を行った。形質調査の結果、各形質の中で穎果の幅が粒重の増加と最も強い正の相関を示した。また、穎果が丸くなることと粒重の増加には正の相関があった。栽培化による粒重の増加には幅の増加、また穎果の形の変化が大きく影響したのではないかと考えられる。, Domestic conference

  Poster presentation


• Quantitative analysis of intra-molecular recombination of mitochondrial genome in wheat

  Ohta Seishi, Makita Masayuki, Tusjimura Mai, Terachi Toru, MORI NAOKI

  第11回ムギ類研究会, Dec. 2016, Japanese, 岡山大学 資源植物学研究所, ミトコンドリアはほとんど全ての真核生物に見られる細胞小器官であり、エネギー生産という重要な役割を担っている。コムギのミトコンドリアは独立した環状のDNA分子をもち、さらにこの分子内の組換えで生じた小環状分子の存在が報告されている。この小環状分子の動態はミトコンドリアの機能と関わりを持つ可能性が有るため、本研究ではコムギにおけるミトコンドリアゲノムの分子内組換え頻度の定量的解析系の確立を試みている。, Domestic conference

  Poster presentation


• 二粒系コムギの栽培化に伴って変化した頴果の大きさや形態の遺伝学的解析

  Miyazaki Yuki, Pham Minh Ngoc, Liberatore Katie, Kianian Shahryar, MORI NAOKI

  近畿作物育種研究会 第182回例会, Nov. 2016, Japanese, 近畿大学生物理工学部, ＜はじめに＞ コムギは西南アジアにおいて倍数性進化と栽培化によって起源したと考えられている。約1万年前、人間は野生エンマーコムギ (Triticum turgidum ssp. dicoccoides)を利用し始めたが、その過程で栽培化が起こり、栽培エンマーコムギ (T. turgidum ssp. dicoccum)が生まれたとされている。栽培化により脱粒性の喪失、頴果の重量の増加などが起こった。その結果、コムギの収穫効率や収量は向上し、農耕の発展に大きく寄与したと思われる。エンマーコムギの野生種と栽培種の間では脱粒性以外にも多くの形質で違いが見られるが、それらの遺伝的制御機構の全貌については不明な点が多い。我々は、エンマーコムギの栽培化関連形質に関与するQTLの同定を目的として研究を進めている。今回は、頴果の大きさや形に関与するQTLの位置や効果, Domestic conference

  Oral presentation


• Search for wild tetraploid wheat populations with non-dormant individuals at a high frequency

  Ohta Shoji, MORI NAOKI, Ozkan Hakan

  129th Annual Meeting of the Plant Breeding Society of Japan, Mar. 2016, Japanese, Yokohama City University, 種子非休眠性の栽培コムギ集団の成立過程を解明する目的で、栽培化起源地域であるトルコ南部の自然集団10集団で収集した野生四倍性コムギ（集団あたり約50個体）を、2014年11月から2015年6月にチュクロワ大学で比較栽培した後、2015年11月末から12月初に各個体の穎果の重さと発芽率および発芽指数を調査した。その結果、自然集団には栽培型と同様に小穂内に大きさの揃った穎果を着ける非休眠性の個体が存在し、そのような個体を高頻度で含む集団があることが明らかとなった。この結果から、栽培化に伴う栽培コムギ集団の成立について考察した。, Domestic conference

  Oral presentation


• Exploring the roots of Japanese Sake brewing rice

  MORI NAOKI

  橿原昆虫館 サイエンスカフェ, Mar. 2016, Japanese, 奈良県立橿原昆虫館, 橿原昆虫館, 日本酒は古くから我国の伝統的な技術と材料を用いて作られてきた。酒造工程で用いられる酒米はできあがる酒の品質を大きく左右するため、よりよい酒米品種を追求する先人たちによって「意識的」にあるいは「無意識のうちに」選ばれてきた一群のイネ品種群であると考えられるが、その起源はよくわかっていない。全国各地に伝わる「酒米品種」のもつ遺伝的多様性とルーツを明らかにするため、兵庫県立農林水産技術総合センター・酒米試験地と共同研究を行い、全国各地の酒米についてそれらのDNAにどれくらいの違いがあるのか調査した。その結果、食用米と比べて酒米の遺伝的多様性は小さく、祖先に当たる品種の数もごく限られたものであった可能性が高いということ、また、酒米はもともと関西地方で生まれ、その後全国各地に拡がったのではないかということが明らかになった。, Domestic conference

  Public discourse


• Sequence analysis of the type VIIb mitochondrial genome found in spelt wheat

  Tsujimura Mai, MORI NAOKI, Terachi Toru

  129th Annual Meeting of the Plant breeding society of Japan, Mar. 2016, Japanese, Japanese Society of Breeding, Yokohama City Universiy, Bゲノムを持つ倍数性コムギには2タイプのミトコンドリアゲノム(VIIa型とVIIb型)が存在し、両者はHindIIIによる１つの制限断片で明瞭に区別される。これまでの解析から、VIIb型には約9.5kbpの特異的な配列が存在することがわかっている（Tsujimura et al. 2013）。 本研究ではVIIb型ミトコンドリアゲノムの全貌を明らかにするため、NGSを用いて全ゲノムの解読を行った。また、既報のVIIa型と比較して、SNPsの検出を行い、両者の違いを明確にすることを試みた。, Domestic conference

  Oral presentation


• 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の解析

  Shimada Saori, Gyu Koichi, Vladutu Christian, Ishii Takashige, Kianian Shahryar, MORI NAOKI

  第10回 ムギ類研究会, Dec. 2015, Japanese, 三重大学農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連形質の解析を行った。その結果、まだメーカー数が藤生部であるが穂軸の脆弱性や１穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, Domestic conference

  Poster presentation


• 栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連QTLの解析

  Miyazaki Yuki, Pham-Minh Ngoc, Vladutu Christian, Ishii Takashige, Kianian Shahryar, MORI NAOKI

  第10回 ムギ類研究会, Dec. 2015, Japanese, 三重大学 農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連QTLの解析を行った。その結果、穂軸の脆弱性や１穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, Domestic conference

  Poster presentation


• コムギのミトコンドリアゲノムの分子内組換えの定量的解析

  Ohta Seishi, Makita Masayuki, Tsujimura Mai, Terachi Toru, MORI NAOKI

  第10回 ムギ類研究会, Dec. 2015, Japanese, ムギ類研究会, 三重大学 農学部, コムギのミトコンドリアゲノムには、大小様々な反復配列が存在し、それらの間で分子内組換えが起こることによって小環状分子種が生じていることがしられている。しかし、分子内組換えの頻度や組織ごとの分子数についてはほとんどわかっていない。本研究ではqPCR法によるこれら組換え分子の量的解析系の開発を試みた。, Domestic conference

  Poster presentation


• Development of backcross inbred lines with genetic background of wild emmer wheat

  Mori Naoki, Gyu Koichi, Vladutu Cristian, Ishii Takashige, Kianian Shahryar

  日本育種学会第１２８回講演会, Sep. 2015, Japanese, 新潟大学, エンマーコムギ(Triticum turgidum ssp. dicoccum)は今から約1万年前に野生二粒系コムギ（別名パレスチナコムギ、T. turgidum ssp. dicoccoides）から栽培化されて誕生した。このコムギは新石器時代から数千年にわたってムギ農耕の主要な作物として利用されたことが知られている。我々はこの栽培化の過程でゲノムにどのような変化が起こったのかを明らかにするため、これまでに栽培種と野生種の交雑に由来するF2集団や栽培種の遺伝的背景に野生種の染色体断片を導入した戻し交雑系統群を用いて解析してきた。今回、栽培化初期の遺伝的変化を捉えることを目的として、野生二粒系コムギの遺伝的背景に栽培エンマーコムギの染色体断片を導入した戻し交雑系統群を作成したので報告する。, Domestic conference

  Poster presentation


• Genetic study of domestication traits in emmer wheat (Triticum turgidum ssp. dicoccum) using backcross derived lines

  MIyazaki Yuki, Pham-Minh Ngoc, Vladutu Cristian, Ishii Takashige, Kianian Shahryar, MORI NAOKI

  日本育種学会第１２８回講演会, Sep. 2015, Japanese, 新潟大学, 約1万年前、人間は野生エンマーコムギ (Triticum turgidum ssp. dicoccoides)を利用し始めたが、その過程で栽培化が起こり、栽培エンマーコムギ (T. turgidum ssp. dicoccum)が生まれた。エンマーコムギの野生種と栽培種の間では脱粒性以外にも多くの形質で違いが見られるが、それらの遺伝的制御機構の全貌については不明な点が多い。本研究では、エンマーコムギの栽培化関連形質に関与するQTLの同定を目的としてエンマーコムギの遺伝的背景に野生エンマーコムギの染色体断片を導入した戻し交雑自殖系統を作出し、DNAマーカーによるQTL解析を行った。その結果、いくつかの栽培化関連形質に関与するQTLについてその位置と効果の大きさを推定できた。, Domestic conference

  Poster presentation


• Chloroplast DNA variation within two natural populations of wild emmer wheat (Triticum turgidum ssp. dicoccoides) in southern Turkey

  Naoki Mori, Takako Shizuka, Hakan Ozkan, 大田 正次

  Annual meeting of the Japanese Society of Plant Breeding, Mar. 2015, Japanese, 多摩川大学, 野生二粒系コムギ(Triticum turgidum ssp. dicoccoides) は栽培二粒系コムギの祖先野生種であり、コムギの育種において重要な遺伝資源である。この野生種は西南アジアのいわゆる肥沃な三日月地帯に分布しており、トルコ南部はこのコムギが栽培化された地域として注目されている。我々は過去10年以上にわたりトルコ南部の野生二粒系コムギを調査してきた。このコムギの自然集団内の遺伝的変異と遺伝的構造を明らかにするため、トルコ南部の2集団を選び、それぞれの集団内を６から８の区画に分けて、それぞれの区画から採集したサンプルを用いて葉緑体ゲノムのマイクロサテライト座の変異を調査した。この結果、トルコ南部の野生二粒系コムギは集団内に非常に高い変異を保有しているが、その変異の空間的分布には大きな偏りがあることが判明した。, Domestic conference

  Oral presentation


• Genetic analysis of domestication traits in emmer wheat using backcross inbred lines

  MORI NAOKI, Ngoc Pham Minh, Vladutu Cristian, Thanh Pham Thien, Thanh Phan Thi, Takashige Ishii, Kianian Shahryar

  Annual meeting of the Japanese Society of Plant Breeding, Sep. 2014, Japanese, 南九州大学, エンマーコムギ (Triticum turgidum ssp. dicoccum)は、約１万年前に野生種 (パレスチナコムギ、T. turgidum ssp. dicoccoides) から栽培化されて誕生した。このコムギは新石器時代から数千年にわたり西南アジアからヨーロッパにかけて主要な穀物として利用されたことが知られている。我々はコムギの栽培化初期の遺伝的変化を明らかにするため、エンマーコムギとパレスチナコムギのF1にエンマーコムギを戻し交雑し、エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を作成した。本講演では、この系統を用いて脱粒性などの栽培化関連形質のQTL解析を行ったので報告する。, Domestic conference

  Poster presentation


• Aegilops cylindricaゲノムの発現に対するAe. caudataプラズモンの遺伝的影響

  TSUNEWAKI KOICHIRO, MORI NAOKI, YOTSUMOTO TATSUYA, TAKUMI SHIGEO

  Annual meeting of the Japanese Society of Genetics, Sep. 2014, Japanese, 長浜バイオ大学, 半世紀にわたりパンコムギゲノム(AABBDD)と共存したAegilops caudataプラズモンをAe. caudata (ゲノム CC)に逆移入してこの種を再構成し、ゲノムに対するプラズモンの遺伝的自律性を検証する研究を進めている。この過程で，パンコムギに共生してきたcaudataプラズモンを橋渡し的にAe. cylindrica（ゲノムCCDD）に移入し、その異種プラズモン系統を育成する必要が生じた。このAe. cylindricaの異種プラズモン系統と正常系統の13形質を5世代に亘って比較分析したところ、部分雄性不稔の誘発のみ認められた。両系統の葉緑体とミトコンドリアゲノムの46 SSR座位の比較では、23座位に差異が見られた。, Domestic conference

  Oral presentation


• Identification of a nuclear genomic region that alters mitochondrial genome type in tetraploid wheats

  TSUJIMUA MAI, MORI NAOKI, YAMAGISHI HIROSHI, TERACHI TORU

  Annual meeting of the Japanese Society of Plant Breeding, Sep. 2014, Japanese, 南九州大学, Bゲノムを持つEmmer-Dinkelコムギのミトコンドリア(mt)ゲノムには、SCARマーカーで区別されるVIIa型とVIIb型がある。このうち4倍性の栽培コムギ、Triticum dicoccumの１系統（dcm1001)はVIIb型のmtゲノムを、４倍性の野生種、T. dicoccoidesの１系統(dcc63)はVIIa型のmtゲノムをそれぞれ持つ。我々はdcm1001にdcc63の花粉を交配して得たF1に、dcm1001あるいはdcc63を２回連続戻し交配し、それらを9回あるいは10回自殖させて、２つのRIL集団を作出した。これあのマーカー遺伝子型を詳細に解析したところ、6B染色体上の8.2cMの領域が、mtゲノムタイプの決定に関与していることがわかった。, Domestic conference

  Oral presentation


• Genetic diversity and domestication of emmer wheat

  MORI NAOKI

  Institute seminar at Cereal Disease Laboratory, United States of America Department of Agriculture (USDA)- Agricultural Research Service (ARS), Aug. 2014, English, Cereal Disease Laboratory, United States of America Department of Agriculture (USDA)- Agricultural Research Service (ARS), Cereal Disease Laboratory, USDA-ARS & Univ. Minnesota, St. Paul, MN, U.S.A., The domestication of wheat and barley was the most important step in the emergence of farming communities that led to the ancient civilisations of Mesopotamia. Several lines of evidence indicate that emmer wheat (Triticum turgidum subsp. dicoccum, genome: AABB, 2n = 28) was the earliest form of domesticated wheat derived from wild emmer (T. turgidum subsp. dicoccoides, genome:, International conference

  Public discourse


• 野生二粒系コムギの自然集団における葉緑体ゲノムの遺伝的多様性と分化

  SHIZUKA TAKAKO, MORI NAOKI, OZKAN HAKAN, OHTA SHOJI

  近畿作物育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学大学院農学研究科, 栽培二粒系コムギ（エンマーコムギ、Triticum turgidum L. ssp. dicoccum）は、約1万年前に西南アジアの「肥沃な三日月地帯」と呼ばれる地域で、野生二粒系コムギ（T. turgidum L. ssp. dicoccoides）から栽培化された。野生二粒系コムギは、現在もこの地域における一年生草本の主要な構成要素の一つである。核及び葉緑体ゲノムの解析から、二粒系コムギの栽培化は、この「肥沃な三日月地帯」の北端に位置するトルコ南部でおこった可能性が高いと考えられている。本研究では、野生二粒系コムギの自然集団内における遺伝的多様性と集団間の分化を明らかにするため、トルコ南部の地理的に近い2集団で採集した個体について葉緑体ゲノムの解析を行った。解析の結果、24座のうち集団30bで14座、31bで15座に遺伝的変異がみられた。各座のア, Domestic conference

  Oral presentation


• Seed dormancy in wild wheat: loss of dormancy associated with increase in seed size in early domestication process

  OHTA SOJI, MORI NAOKI, OZKAN HAKAN

  Annual meeting of the Japanese Society of Plant Breeding, Mar. 2014, Japanese, Tohoku University, 野生コムギの種子休眠性について以下のことを明らかにしてきた。1.野生コムギには種子休眠性に大きな遺伝的変異があり非休眠性の個体も存在する。2.遺伝的に休眠性をもつ野生個体の小穂に2粒着粒したとき、第1小花に休眠性の小さな穎果、第2小花に非休眠性の大きな穎果が着く。小穗に1粒着粒した穎果は非休眠性となる。従って、早く発芽した個体を選んでも非休眠性の遺伝子型は選抜できない。3.2粒着粒した小穂の第1小花の穎果が第2小花の穎果に比べて小さいほど強い休眠性をもつ。4,栽培コムギは遺伝的に非休眠性であり、第1小花に第2小花と同じ大きさ、あるいは第2小花より大きな穎果を着ける。5.穎果の大きさと休眠性は同一あるいは密接に連鎖した2つの主働遺伝子によっておもに支配されている。これらの結果から、栽培化の初期の段階では、揃った大きさの大きな穎果を着ける個体の選択が非休, Domestic conference

  Poster presentation


• 自然集団における野生二倍性コムギ及び四倍性コムギの小穂と頴果の形態的変異

  Oi Saki, Ohta Shoji, Mori Naoki, Ozkan Hakan

  日本育種学会 第124回大会, Oct. 2013, Japanese, 鹿児島大学, 種子休眠性は種子繁殖する一年生植物が環境の不意の変化に対応して集団を存続するために重要な遺伝的形質である。演者らは、これまでに野生コムギの種子休眠性について以下のことを明らかにしてきた。1) 野生一粒系および二粒系コムギでは、2粒着いた小穂の第1小花の穎果が同じ小穂の第2小花の穎果および小穂内に単独で着いた穎果に比べて有意に小さく、休眠性を示すが、他の穎果は非休眠性である。2) 自然集団において、2粒着いた小穂の第1小花の穎果は不斉一に発芽し、その多くが翌年の秋に発芽する。3) 野生コムギの個体間で種子休眠性の程度には大きな変異があり、野生一粒系コムギでは小穂あたりの着粒数が多い個体ほど、野生二粒系コムギでは第1小花の穎果が第2小花に比べて小さい個体ほど遺伝的に強い休眠性をもつ。4) 野生二粒系コムギでは、同じ小穂内に大きさの異なる穎果を着ける形質と, Domestic conference

  Oral presentation


• A mitochondrial polymorphic region affected by nuclear genome constitution of Emmer-Dinkel wheat.

  Tsujimura Mai, Mori Naoki, Yamagishi Hiroshi, Terachi Toru

  日本育種学会 第124回大会, Oct. 2013, Japanese, 鹿児島大学, 核にBゲノムを持つEmmer-Dinkelコムギのミトコンドリア(mt)ゲノムには、11.2kbpのHindIII制限断片(H3断片)の有無により区別される、VIIa型とVIIb型の2タイプが存在する。我々はH3断片とその周辺領域の塩基配列情報からmtDNAを精製せずとも、全DNAからVIIa型とVIIb型を区別可能なSCAR(H3マーカー)を作成した(第119回育種学会)。一方、Emmer-Dinkelコムギの葉緑体ゲノムは、SSRマーカーによりplastgroup IとIIの2つに大別される (Hirosawa et al, 2004)。我々はEmmer-Dinkelコムギにおける細胞質ゲノムの分化の詳細を明らかにするため、これらのマーカーを利用して、55系統についてミトコンドリアと葉緑体のゲノムタイプを調べた。, Domestic conference

  Poster presentation


• Genetic diversity and differentiation of chloroplast genome in wild emmer wheat within and among its natural populations

  Takako hizuka, Naoki Mori, Shoji Ohta, Hakan Ozkan

  日本育種学会 第124回大会, Oct. 2013, Japanese, 鹿児島大学, Southern Turkey is one of the richest regions in terms of the diversity of Triticum species. To clarify its genetic diversity and geographical differentiation, we have been surveying chloroplast genome of natural populations of wild emmer wheat (T. turgidum ssp. dicoccoides) in southern Turkey., Domestic conference

  Oral presentation


• Genetic analysis of domestication traits in emmer wheat using an F2 population

  Naoki Mori, Phan Thi Thanh, Cristian Vladutu, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Shahryar Kianian

  12th International Wheat Genetics Symposium,, Sep. 2013, English, Pacifico Yokohama, Japan, Emmer wheat (Triticum turgidum ssp. dicoccum) was a principal crop in the development and spread of Neolithic agriculture in the Old World. It represents the primitive situation in the domestication of AABB tetraploid wheat. It has non free-threshing (hulled) habit and relatively fragile rachis, although it has non-shattering spike. The more advanced forms of tetraploid whea, International conference

  Poster presentation


• Mitochondrial genome analysis of Triticum and Aegilops species II. Mitochondrial genome of an alloplasmic line of common wheat with Ae. speltoides cytoplasm

  Tsujimura Mai, MORI NAOKI, Yamagishi Hiroshi, Terachi Toru

  日本育種学会 第123回講演会, Mar. 2013, Japanese, 東京農業大学, Ae. speltoidesの細胞質を持つ細胞質置換コムギ（核親はT. aestivum）を材料に、ミトコンドリアゲノムの解析を行った。定法によりミトコンドリアを単離した後、塩化セシウム密度勾配法でmtDNAを精製し、次世代シークエンサー（Roche 454）により塩基配列を得た。その結果、約53Mbの情報量を持つ、約109,000個の有効なリードが得られ、それらは全長の合計約548kbの44のコンティグを構成した。葉緑体DNAのコンタミネーションを除き、mtDNA由来のコンティグをマニュアルで整列化したところ、Ae. speltoidesのmtDNAとして、全長476kbの環状マスターサークルを仮定することができた。 我々はこれまで、倍数性コムギの進化に関わる植物のうち、T. aestivumミトコンドリアゲノム（Ogihara et al. 2, Domestic conference

  Poster presentation


• Genetic diversity and origin of emmer wheat and Indian dwarf wheat in Indian subcontinent revealed by chloroplast and nuclear DNA markers

  Nimi Yukiko, MORI NAOKI, Ohta Shoji, Chiba Hajime, Vasant Shinde, 長田 俊樹

  日本育種学会 第122回講演会, Sep. 2012, Japanese, 京都産業大学, 紀元前2600年頃南アジアに興ったインダス文明では、この地域に特有の気候を巧みに利用した農業が営まれ、エンマ―コムギ(T. turgidum ssp. dicoccum)やこの地域に固有の普通系コムギであるインド矮性コムギ(T. aestivum ssp. sphaerococcum)が重要な冬作物として利用されていたと考えられている(Weber 1999)。我々は、地球環境学研究所プロジェクト「環境変化とインダス文明」の一環として、これらのコムギの起源と伝播について考察するために、葉緑体ゲノムの遺伝的多様性を探ってきた。本報告では最近の調査で新たに採集した系統を加え、核ゲノムのDNAマーカーを併用して解析したので報告する。, Domestic conference

  Poster presentation


• Analysis of the origin of Triticum aestivum sap. shphaerococcum using genome-specific STS markers

  Asakura Nobuaki, MORI NAOKI, Nakamura Chiharu, Ohtsuka Ichiro

  日本育種学会 第１２２回講演会, Sep. 2012, Japanese, 京都産業大学, 普通系コムギは二粒系コムギ(T. turgidum L.)と近縁野生種であるタルホコ ムギ(Aegilops tauschii Coss.)との交雑により成立したことが判明しているが、 いつ、どこで、何回、普通系コムギは生じたかについては未だ不明な点が多い。 これらの疑問を解明する一つの方法が、亜種の系統解析である。 Talbertら(1998)はDゲノム特異的STSマーカーであるA1座には二つのアリ ル(A型とB型)があることを見出した。Asakuraら(2011)は、インド矮性コ ムギを含む普通系コムギにおけるA1座の解析から、インド矮性コムギはA1座の A型アリルを持つパンコムギから生じた可能性が高いことを報告している。 Blakeら(2004)は、ADP glucose pyrophosphorylase大サブユニットのBゲノ ムコピー(Ag, Domestic conference

  Oral presentation


• Molecular genetic analysis of the QTLs involved in the domestication of emmer wheat

  Thanh P. T, C. Vladutu, S. Kianian, T. Ishii, T. Pham, S. Nasuda, M. Nitta, N. Mori

  日本育種学会 第122回講演会, Sep. 2012, English, 京都産業大学, Significant association between wheat and human began in southwest Asia more than 10000 years ago. Emmer wheat was one of the founder crop that was domesticated from wild emmer wheat. In the present study, QTL analysis was carried out to estimate the valuable QTLs underlying the domestication of emmer wheat., Domestic conference

  Oral presentation


• Genetic diversity of wild emmer wheat in the natural populations in southern Turkey revealed by microsatellite analysis of chloroplast DNA

  Takako Shizuka, Naoki Mori, Shoji Ohta, Hakan Ozkan

  日本育種学会 第122回講演会, Sep. 2012, English, Kyoto Sangyo University, Shizuka T., N. Mori, S. Ohta, H. Ozkan (2012) . 日本育種学会、京都産業大学、９月, Domestic conference

  Poster presentation


• Aegilops caudata、その細胞質をもつ細胞質置換コムギ及び両者の核と細胞質から再構成されたAe. caudataのオルガネラDNAの比較マイクロサテライト分析

  Tatsuya Yotsumoto, Naoki Mori, TAKUMI SHIGEO, Koichiro Tsunewaki

  日本育種学会, Sep. 2012, Japanese, 京都産業大学, Domestic conference

  Poster presentation


• Mitochondrial genome analysis of Triticum and Aegilops species I. Mitochondrial genome of an alloplasmic line of common wheat with T. timopheevi cytoplasm

  Tsujimura Mai, MORI NAOKI, Tomioka Etsuko, Terachi Toru

  annual meeting of the Japanese Society of Plant Breeding, Mar. 2012, Japanese, Japanese Society of Plant Breeding, コムギ属植物の中でＧゲノムをもつ4倍体であるチモフェービコムギのミトコンドリアゲノムの全塩基配列を解析したのでその詳細を報告した。, Domestic conference

  Oral presentation


• Chloroplast DNA variation of Indian dwarf wheat and emmer wheat currently collected in India

  Nimi Yukiko, MORI NAOKI, Chiba Hajime, S. Vasant, Ohta Shoji, Osada Toshiki

  Japanese Society of Plant Breeding, Mar. 2012, Japanese, Utsunomiya University, 紀元前2600年頃から南アジアに興ったインダス文明では、この地域に特有の機構を宅見に利用した農業が営まれ、エンマーコムギやインド矮性コムギは重要な冬作物として利用されていたと考えられる。本発表では新たにインドで採集したエンマーコムギとインド矮性コムギの遺伝的多様性を明らかにし、インド亜大陸におけるこれらの起源と伝播を考察するため、葉緑体ゲノムの変異を調査した。, Domestic conference

  Poster presentation


• Seed dormancy and germination strategy of wild diploid and tetraploid wheat revealed from the observation at their natural population in southern Turkey

  Ohta Shoji, MORI NAOKI, Kariyasu Rie, Yamada Chinatsu, H. Ozkan

  日本育種学会 第121回講演会, Mar. 2012, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• Seed dormancy and germination strategy of wild diploid and tetraploid wheat revealed from the observation at their natural population in southern Turkey

  Shoji Ohta, Naoki Mori, Rie Kariyasu, Chinatsu Yamada, Hakan Ozkan

  Japanese Society of Plant Breeding, Mar. 2012, Japanese, Utsunomiya University, Seed dormancy is one of the essential genetic characters for wild seed propagated plant species to maintain their populations under a variable environment. In December of 2010 and 2011, we surveyed a total of seven natural populations in Kartal Dagi region of southern Turkey to observe the germination of wild diploid and tetraploid wheat species., Domestic conference

  Oral presentation


• インドにおけるエンマーコムギとインド矮性コムギの遺存的栽培と利用

  MORI NAOKI

  ムギ類研究会, Nov. 2011, Japanese, 横浜市立大学木原生物学研究所、横浜, Domestic conference

  [Invited]

  Invited oral presentation


• 山田錦の両親系統における酒造好適性にかかわる染色体領域の推定

  Hachikenn Takehiro, Yoshida Shinya, Ishii Takashige, Ohta Jyun, Akashi Takahiro, Nishimura Akira, MORI NAOKI

  日本育種学会 第120回講演会, Sep. 2011, Japanese, 福井県立大学、福井, Domestic conference

  Poster presentation


• プラズモン半世紀ぶりに古巣に帰る：パンコムギ核と50世代共生してきたAegilops caudataプラズモンの逆移入によるAe. caudataの再構成

  常脇 恒一郎, MORI NAOKI, 宅見 薫雄

  日本遺伝学会 第83回講演会, Sep. 2011, Japanese, 京都大学，京都, Domestic conference

  Oral presentation


• プラズモン半世紀ぶりに古巣に帰る：パンコムギ核と50世代共生してきたAegilops caudataプラズモンの逆移入によるAe. caudataの再構成

  常脇 恒一郎, MORI NAOKI, TAKUMI SHIGEO

  日本遺伝学会, Sep. 2011, Japanese, 京都大学, Domestic conference

  Oral presentation


• オルガネラゲノムの変異からみたコムギ近縁野生種Aegilops umbellulata、Ae. neglecta及びAe. columnarisの多様性と系統関係

  MORI NAOKI, 八杉 有香, 大田 正次

  日本育種学会 第120回講演会, Sep. 2011, Japanese, 福井県立大学、福井, Domestic conference

  Oral presentation


• インドにおけるエンマーコムギの栽培と伝統的利用

  大田 正次, MORI NAOKI, 千葉 一, V. Shinde, 長田 俊樹

  日本育種学会 第120回講演会, Sep. 2011, Japanese, 福井県立大学、福井, Domestic conference

  Poster presentation


• The two ancient wheats, emmer wheat and Indian dwarf wheat, are still alive in India - their cultivation and utilization-

  Shoji Ohta, MORI NAOKI, Hajime Chiba

  Indus Project International Symposium 2011: “Environmental Change and the Indus Civilization”, Aug. 2011, English, Research Institute for Humanity and Nature, Kyoto, Japan, International conference

  [Invited]

  Invited oral presentation


• Chloroplast DNA variation in emmer wheat and Indian dwarf wheat in Indian subcontinent

  Naoki Mori, Toshiya Takagi, Hajime Chiba, Shoji Ohta

  Indus Project International Symposium 2011: “Environmental Change and the Indus Civilization”, Aug. 2011, English, Research Institute for Humanity and Nature, Kyoto, Japan, International conference

  [Invited]

  Invited oral presentation


• 野生四倍性コムギにおける種子休眠性の小穂内二型性—トルコ東南部の自然集団における発芽調査

  Ohta Shoji, MORI NAOKI, H. Ozkan

  日本育種学会 第119回講演会, Mar. 2011, Japanese, 横浜市立大学、横浜, Domestic conference

  Oral presentation


• 倍数性コムギのミトコンドリアゲノムに見られる構造変異とその分布

  辻村 真衣, MORI NAOKI, 寺地 徹

  日本育種学会 第119回講演会, Mar. 2011, Japanese, 横浜市立大学，横浜, Domestic conference

  Oral presentation


• Rediscovery of the relict cultivation of the ancient Indus crop, Indian dwarf wheat, in southwestern India

  森 直樹, 大田正次, 千葉一, V. Shinde, M. Kajale, 長田俊樹

  日本育種学会 第119回講演会, Mar. 2011, Japanese, 横浜市立大学、横浜, Domestic conference

  Oral presentation


• Genetic diversity in wheat. II.

  Naoki Mori

  Special lecture on genetic diversity of crop species, Nov. 2010, English, Chiang Mai University, Thailand, International conference

  Public discourse


• Genetic diversity in wheat. I.

  Naoki Mori

  Special Lecture on genetic diversity of crop species, Nov. 2010, English, Kasetsart University, Thailand, International conference

  Public discourse


• Evolution and domestication of wheat species inferred by chloroplast DNA fingerprinting. II.

  Naoki Mori

  Special Seminar on genetic diversity of crop species, Nov. 2010, English, Chiang Mai University, Thailand, International conference

  Public discourse


• Evolution and domestication of wheat species inferred by chloroplast DNA fingerprinting. I.

  Naoki Mori

  Special seminar on genetic diversity of crop species, Nov. 2010, English, Kasetsart University, Thailand, International conference

  Public discourse


• 葉緑体ゲノムの遺伝的変異からみたコムギ近縁野生種Aegilops umbellulataの多様性とAe. neglecta及びAe. columnarisの倍数性進化

  Yasugi Yuka, Mori Naoki, Ohta Shoji

  日本育種学会 第118回講演会, Sep. 2010, Japanese, 秋田県立大学、秋田, Domestic conference

  Poster presentation


• 倍数性コムギの進化に新しい知見をもたらすミトコンドリアゲノムの構造変異

  辻村 真衣, 冨岡 閲子, MORI NAOKI, 寺地 徹

  日本育種学会第188回講演会, Sep. 2010, Japanese, 秋田県立大学、秋田, ，，森直樹，(2010) . 、、９月., Domestic conference

  Oral presentation


• アビシニア高地を中心とした栽培エンマーコムギの遺伝的変異

  竹中 祥太朗, MORI NAOKI, 河原 太八

  日本育種学会、第188回講演会, Sep. 2010, Japanese, 秋田県立大学、秋田, Domestic conference

  Oral presentation


• Genetic diversity of emmer wheat and Indian dwarf wheat in India inferred from chloroplast DNA analysis

  Takagi Toshiya, MORI NAOKI, Ohta Shoji, Chiba Hajime, Shinde Vasant, Kajale Mukund, Osada Toshiki

  日本育種学会 第118回講演会, Sep. 2010, English, 秋田県立大学、 秋田, Domestic conference

  Poster presentation


• Dゲノム特異的STSマーカーA1の塩基配列からみたTriticum aestivum ssp. sphaerococcumの起源

  朝倉 史明, MORI NAOKI, 中村 千春, 大塚 一郎

  日本育種学会 第118回講演会, Sep. 2010, Japanese, 秋田県立大学、秋田, Domestic conference

  Poster presentation


• アビシニア高地を中心とした４倍性コムギ(Triticum turgidum L.)の遺伝的変異と地域分布

  Takenaka Shotaro, MORI NAOKI, Kawahara Taihachi

  日本育種学会第117回講演会, Mar. 2010, Japanese, 京都大学、京都, Domestic conference

  Poster presentation


• 葉緑体DNAのSSR多型からみたインド在来コムギの遺伝的多様性

  高木俊哉, MORI NAOKI

  ムギ類研究会, Nov. 2009, Japanese, 芦原、福井, Domestic conference

  Poster presentation


• コムギ近縁野生種Aegilops neglectaおよびAegilops columnarisのオルガネラゲノムの多様性と自生地における地理的変異

  Yasugi Yuka, MORI NAOKI

  ムギ類研究会, Nov. 2009, Japanese, 芦原、福井, Domestic conference

  Poster presentation


• QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

  Pham Thien Thanh, Ryo Ishikawa, Naoki Mori, Takashige Ishii

  6th International Conference of Rice Genetics, Nov. 2009, English, Manila Hotel, Philippine, International conference

  Poster presentation


• QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

  Thanh PT, ISHIKAWA RYO, MORI NAOKI, ISHII TAKASHIGE

  International Rice Genetics Symposium, Nov. 2009, English, Manila, Philippines, International conference

  Poster presentation


• Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species

  MORI NAOKI, Hidehira, Y, Ishii, T, Nakamura, C

  4th International Conference on Energy Efficiency and Agricultural Engineering, Oct. 2009, English, Rousse, Bulgaria, International conference

  Oral presentation


• 易脱穀性二粒系コムギの葉緑体ゲノムの遺伝的変異

  Takagi Toshiya, Mori Naoki, Kawahara Taihachi, Nakamura Chiharu

  日本育種学会 第116回講演会, Sep. 2009, Japanese, 北海道大学, Domestic conference

  Poster presentation


• コムギ近縁野生種Aegilops neglectaおよびAegilops columnarisの葉緑体ゲノムの多様性と自生地における地理的変異

  Yasugi Yuka, MORI NAOKI, Ohta Shoji

  日本育種学会 第116回講演会, Sep. 2009, Japanese, 日本育種学会, 北海道大学, Domestic conference

  Poster presentation


• コムギ近縁野生種Aegilops neglecta-Ae. columnaris種複合における遺伝的変異と地理的分布 – 倍数性、形態及び雑種不稔性

  Ohta Shoji, Fujita Yuko, Maesaka Yoshikazu, Iwasaki Rie, MORI NAOKI, Ozkan Hakan

  日本育種学会 第116回会講演会, Sep. 2009, Japanese, 北海道大学, Domestic conference

  Poster presentation


• Aegilops caudataプラズモンを持つAe. cylindricaの作出とその特性

  Tsunewaki Koichiro, MORI NAOKI, Takumi Shigeo

  日本遺伝学会 第81回講演会, Sep. 2009, Japanese, 信州大学, ，森直樹， (2009) . 日本遺伝学会、信州大学、９月., Domestic conference

  Oral presentation


• Intraspecific variation of chloroplast DNA in Aegilops speltoides

  Naoki Mori, Hiroyuki Watatani, Takashige Ishii, Yasutaka Kondo, Taihachi Kawahara, Chiharu Nakamura

  6th International Triticeae Symposium, Jun. 2009, English, Kyoto University, Kyoto, Japan, International conference

  Poster presentation


• Allelic diversity at chloroplast microsatellite loci among polyploid wheat species

  Naoki Mori

  6th International Triticeae Symposium, Jun. 2009, English, Kyoto University, Kyoto, Japan, International conference

  [Invited]

  Invited oral presentation


• Screening of BPH resistance/infestation-associated genes by means of AMF.

  Gamalath N.S, MORI Naoki, Muhammad N, NAKAMURA Chiharu

  5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, Nov. 2005, English, 未記入, Manila, Philippines, International conference

  Oral presentation


• Profiling of endosperm gene expression in Sake-brewing rice by SAGE.

  TAKAHASHI T, KAWAMURA M, YOSHIDA S, MATSUMURA H, TERAUCHI R, NAKAMURA Chiharu, MORI Naoki

  5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, Nov. 2005, English, 未記入, Manila, Philippines, International conference

  Oral presentation


• Identification of simple and effective bioassay method for submergence tolerance in rice (Oryza sativa).

  Manangkil O, Vu Thi Thu H, MORI Naoki, YOSHIDA S, NAKAMURA Chiharu

  5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, Nov. 2005, English, 未記入, Manila, Philippines, International conference

  Oral presentation


• Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by molecular markers.

  MORI Naoki, HASHIMOTO Z, YOSHIDA S, KAWAMURA M, ISHII T, IKEGAMI M, TAKUMI Shigeo, KAMIJIMA Osamu, NAKAMURA Chiharu

  5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, Nov. 2005, English, 未記入, Manila, Philippines, International conference

  Oral presentation


• Differential responses of three brown planthopper populations to japonica rice introgression lines carrying Bph1 or bph2 resistance genes.

  Naeemullah N, MATSUMURA M, MORI Naoki, TAKEDA Makio, NAKAMURA Chiharu

  5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, Nov. 2005, English, 未記入, Manila, Philippines, International conference

  Oral presentation


• コムギ近縁野生種Aegilops neglectaとAe. columnarisのトルコ南部における地理的分布

  大田 正次, HAKAN Ozkan, 森 直樹

  日本育種学会第107 ・108 回講演会, Aug. 2005, Japanese, 日本育種学会, 筑波大学, Domestic conference

  Oral presentation


• コムギにおけるMutator様転移酵素配列の同定とイネ科植物内での分布

  朝倉 史明, 吉田 晋弥, 森 直樹, 大塚 一郎, 中村 千春

  日本育種学会第107 ・108 回講演会, Aug. 2005, Japanese, 日本育種学会, 筑波大学, Domestic conference

  Oral presentation


• コムギ-エギロプス属２倍体種のPolA1 遺伝子第19イントロンのDNA 多型

  高橋 弘子, 森 直樹, 河原 太八, 中村 郁郎

  日本育種学会第107 ・108 回講演会, Aug. 2005, Japanese, 日本育種学会, 筑波大学, Domestic conference

  Oral presentation


• 普通糸コムギならびにその祖先種でみられたコムギミトコンドリアマイクロサテライト座における多様性

  高橋 知佐, 森 直樹, 上島 脩志, 石井 尊生

  近畿作物・育種研究会第159回例会, 2005, Japanese, 日本育種学会, 神戸大学, Domestic conference

  Oral presentation


• 葉緑体DNAのマイクロサテライト多型からみたエンマーコムギと裸性二粒系コムギの遺伝的分化

  森 直樹, 田中 知秋, 秀平 裕子, 綿谷 浩之, 河原 太八, 中村 千春

  日本遺伝学会第76回講演会, Sep. 2004, Japanese, 日本遺伝学会, 大阪大学, Domestic conference

  Oral presentation


• 二粒系コムギの栽培化と普通系コムギの進化

  森 直樹

  日本育種学会第106回講演会, Sep. 2004, Japanese, 日本育種学会, 三重大学, Domestic conference

  Oral presentation


• 現存するコムギのDNAからみた栽培コムギの起原

  森 直樹

  地球環境研究所シンポジウム, Sep. 2004, Japanese, 未記入, 国際日本文化研究センター, Domestic conference

  Oral presentation


• イネ幼病期低温耐性のQTL解析

  Aloka Lanka Ranawake, 森 直樹, 青木 法明, 梅本 貴之, 吉田 晋也, 中村 千春

  日本育種学会第106回講演会, Sep. 2004, Japanese, 日本育種学会, 三重大学, Domestic conference

  Oral presentation


• 現世コムギのDNAからみた栽培コムギの起原

  森 直樹

  地球環境研究所シンポジウム, Jul. 2004, Japanese, 未記入, 国際日本文化研究センター, Domestic conference

  Oral presentation


• 祖先２倍種の葉緑体DNA における分子多型から見た４倍性コムギの起原

  綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹

  日本育種学会 育種学研究, Sep. 2003, Japanese, 日本育種学会, 神戸大学, Domestic conference

  Oral presentation


• AMF とSAGE による酒米の胚乳発達過程における遺伝子発現の解析

  川村 雅志, 寺内 良平, 松村 英生, 吉田 晋弥, 中村 千春, 森 直樹

  日本育種学会 育種学研究, Sep. 2003, Japanese, 日本育種学会, 神戸大学, Domestic conference

  Oral presentation


• ペレニアルライグラスいもち病菌の地理的機嫌の解明とコムギに対する発病ポテンシャルの評価

  中川 志織, 土佐 幸雄, 平田 健治, 中馬 いづみ, 磯部 千尋, 中屋敷 均, 森 直樹, WAKAR Uddin, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• 祖先２倍種の葉緑体DNA における分子多型からみた４倍性コムギの起原

  綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹

  日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference

  Oral presentation


• Origin of domesticated emmer and common wheat inferred from chloriplast DNA finger printing.

  MORI Naoki, ISHII T, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA S, NAKAMURA Chiharu

  Ⅹth Int. Wheat Genet. Symp., 2003, English, 未記入, Paestum, Italy., International conference

  Oral presentation


• Mitochondrial genome and genes of common wheat, Triticum aestivum cv. Chinese Spring.

  OGIHARA Y, FUTO S, KANNO A, MIYASHITA N, NASUDA S, SHIINA T, TERACHI T, GUO C-H, NAKAMURA Chiharu, MORI Naoki, TAKUMI Shigeo, MURATA M, YAMAZAKI Y, MURAI K, MATSUOKA Y, TSUNEWAKI K

  Ⅹth Int. Wheat Genet. Symp., 2003, English, 未記入, Paestum, Italy., International conference

  Oral presentation

• 日本進化学会

  2020 - Present


• 米国遺伝学会


• 日本育種学会


• 日本遺伝学会

• 野生コムギの生存戦略としての特異的休眠機構の解明と遺伝資源保全への応用

  Naoki Mori, Yoshihiro Matsuoka

  JSPS, 科学研究費助成事業, 基盤研究 (B) 一般, Graduate School of Agricultural Science, Apr. 2023 - Mar. 2028, Principal investigator


• 野生コムギ集団に眠る未知の多様性の発掘と評価：栽培化過程解明と育種利用基盤構築

  森 直樹

  学術研究助成基金助成金／国際共同研究加速基金(国際共同研究強化(B)), Oct. 2018 - Mar. 2023, Principal investigator

  Competitive research funding


• 擬似自然集団群の育成による野生コムギ遺伝資源の保全とその持続的・発展的利用

  森 直樹

  学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• 植物ミトコンドリアゲノムの進化学的安定性と発生学的動態の解明

  森 直樹

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2013 - Mar. 2016, Principal investigator

  Competitive research funding


• Analysis of genetic diversity in plants with wide distribution in Eurasia by using wild relatives of wheat

  KAWAHARA Taihachi, YASUI Yasuo, TAKUMI Shigeo, MATSUOKA Yoshihiro, MORI Naoki, YAMANE Kyouko, SASANUMA Tsuneo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kyoto University, 2005 - 2008

  トルコから中国西部にかけて, ユーラシア大陸内陸部に広域く分布するタルホコムギ(Aegilops tauschii)について, 種内の葉緑体塩基配列・穂の形態・開花までの日数などについて変異を明らかにした。解析の結果同じような気候条件に対し, 分布の東と西で違う遺伝子の変異によって適応していることが分かったので, これらの遺伝子について塩基配列を決定しその変異を明らかにした。また乾燥に応答する遺伝子など, そのほかいくつかの遺伝子の変異を明らかにした。


• Evaluation of Genetic Diversity in and Molecular Genotyping of Asian Wheat Genetic Resources

  SASANUMA Tsuneo, MIURA Hideho, KAWAHARA Taihachi, MORI Naoki, KATO Kenji

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Yokohama City University, 2004 - 2006

  Evaluation of genetic diversity in and molecular genotyping of genetic resources of wheat and its relatives were conducted. Modernization of agriculture has produced many modern wheat cultivars adapted with irrigation, chemical nutrition and mechanization, and today supported a huge world food demand. The modernization, however, resulted in loss of genetic diversity, thus we face the limitation of the increased yield and fragility against coming world environment changes. To overcome the problems, the genetic diversity present in wild species and are reconsidered, and the importance of evaluation and utilization of the potential genetic diversity are noticed. The establishment of the evaluation method of such diversity and the elucidation of the evolutionary process which has produced the diversity are also considered to be valuable. The spread of cultivated wheat from its original place to the west and east produced a lot of differentiated local varieties, namely landraces, adapted with various local climates. Japanese wheat research community have collected wild species and landraces and have long-time conserved them as genetic resources. This research project is a collaborating research formed by researchers who have conserved the genetic resources and investigated genetic diversity or evolutionary processes. In this project approximately 600 cultivated and 700 wild accessions were investigated and the following outcomes were obtained. 1. The basic information of morphology, heading trait, salt tolerance and seed dormancy for each accession were evaluated and recorded. The level of diversity was considerably high in both wild and cultivated species. 2. Core collections (representative accessions covering whole population) were selected based on DNA marker and AFLP, and methods for conservation were proposed. 3. Multiple origins in both domestication and polyploidization processes were revealed, which are clearly contrasted with previous theories. 4. The genes controlling or related with heading trait and seed dormancy were identified using DNA marker. Genetic variation among accessions in those genes was revealed. These results enable us to use DNA marker to distinguish variety or to reveal evolutionary process. 5. The accessions used in this study and data obtained were registered in National BioResources Project (NBRP) database and free access and easy utilization were enabled.


• A study on the interrelationships among environment, people, crops and weeds in the agro-ecosystem based on wheat and barley cultivation

  OHTA Shoji, TOMINAGA Tohru, HONGO Akio, MORI Naoki, MATSUOKA Yoshihiro, OHSAKO Takanori

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Fukui Prefectural University, 2003 - 2006

  1.Summary of field researches and collection : (1)A series of cooperative field researches were carried out with Cukurova University. A total of 1709 samples including Triticeae, Lolium, and so on, were collected. Germination of some Aegilops species were observed at their natural populations in October and December. (2)A series of cooperative field researches were carried out in the northern and northwestern parts of Iran, based on the agreement with the National Plant Gene-Bank of Iran (NPGBI). A total of 1557 samples including Triticeae, Lolium, Medicago, and so on, were collected. A part of samples of Lolium and Secale were received by Japanese members based on the MTA between NPGBI and Fukui Prefectural University. (3)A field research was carried out in northern Greece. A total of 54 samples including Lolium, Brassicaceae, and so on, were collected. 2.Ecotype differentiation in Aegilops species : (1)It was revealed that the two varieties of Ae. neglecta and Ae. columnaris grow in ecologically different habitats, and that var. contorta of Ae. neglecta is distributed in southwestern Turkey, with no description so far, and its distribution is expanding with an intensive human activity. (2)It was revealed that ssp. strangulata and var. meyeri of Ae. tauschii are the ecotypes adapted to calcareous and sandy soils, respectively, in the southern coastal plains of the Caspian Sea. (3)It was revealed by field observations that Aegilops species realized both rapid germination for the establishment of populations and variation in seed germination against unpredictable disasters by bearing tow types of grains with different dormancy on the same spikes. 3.Genetic variation and association modes of associated weeds in wheat and barley cultivation : (1)It was revealed that Lolium temulentum and L.persicum grow sympatrically on road-side in the northwestern parts of Iran. A part of collected material is now growing at Kyoto University for the further genetic analysis. (2)It was revealed that weed rye with brittle spikes commonly grows in wheat and barley fields in Iran, and the frequency is high in the centers of genetic diversity of cultivated rye. The plants with standard B-chromosomes were found in five cultivated and weed rye populations in Iran. Introgression between cultivated-weed rye (Secale cereale) and wild perennial rye (S.montanum) was found in northern Iran. 4.Establishment of common wheat : It was revealed that durum wheat is still cultivated in some villages of the Alborz Mountains and Aegilops tauschii grows at the margin of the durum wheat fields. 5.Invitation of Turkish members : A graduate student and an associated professor of Cukurova University were invited to Fukui Prefectural University, Kyoto University and Kobe University in April-May,2005 and September,2006,respectively.


• 酒造好適性を支配する遺伝因子の同定とそれに基づく酒米遺伝資源の評価技術の開発

  上島 脩志

  科学研究費補助金／基盤研究(B), 2005

  Competitive research funding


• STUDY ON THE COLD-RESPONSIVE PATHWAYS IN POLYPLOID WHEAT

  NAKAMURA Chinaru, TAKUMI Shigeo, MORI Naoki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kobe University, 2001 - 2003

  Cold/freezing tolerance or winter hardiness is an important trait for over-wintering plants. Plants grown in the temperate regions have ability to respond and adapt to low temperature in order to survive severe winter. This adaptive ability manifests itself through the process known as cold hardening or acclimation, which involves significant alternations in gene expression profiles associated with numerous biochemical and physiological changes. Our aim of this research was to understand the cold-responsive pathways in wheat. We cloned a number of cold-responsive (Cor/Lea) genes and their putative transcription factors, and compared their expression profiles with the developmental time-course of freezing tolerance during cold acclimation of two wheat cultivars with contrasting levels of freezing tolerance. Our major findings can be summarized as follows. (1) The developmental time course of freezing tolerance shows good correlation with the expression profiles of Cor/Lea genes and a transcription factor Cbf/Dreb1 during cold acclimation. (2) Expression of Cbf/Dreb1 is rapid (within 15 min after exposure to low temperature at 4℃), preceding that of the down-stream Cor/Lea genes. (3) There are two apparent peaks of expression of Cbf/Dreb1 and Cor/Lea genes, i.e., an early response peak at 1-4 hours and a long-term response peak at 2-3 weeks after cold acclimation. (4) Elevated levels of expression of Cbf/Dreb1 and Cor/Lea genes are associated with the recessive state of vernalization responsive genes vrn1, thus likely with the dominant state of frost/freezing resistance genes Fr1. (5) A major cold-responsive pathway found in Arabidopsis is conserved in the wheat genome. (6) The wheat genome possibly possesses a novel cold-responsive pathway(s).


• STUDIES ON GENETIC VATIATION IN RELATIVES OF WHEAT USING MOLECULAR MARKERS

  KAWAHARA Taihachi, MORI Naoki, YASUI Yasuo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), KYOTO UNIVERSITY, 2000 - 2002

  a. Isozyme: Analysis of isozymes were nude in three species of Aegilops. In Ae. umbellulata, geographical cline of enzyme loci was found. Morphological differentiation was detected between accessions from Aegean Islands and those from Turkey and Iran. b. AFLP: AFLP analysis was successfully made in common wheat and its ancestors, emmer wheat and Ae. tauschii. We found clearly a cluster of macha and spelta, respectively. While, aestivum and comopactum formed one claster. c. Fingerprinting of cpDNA: We examined 24 SSR loci in chloroplast DNA. We found two maternal lineages in common wheat indicating that common wheat has originated independency at least twice. From the comparison of cpDNA of wild and cultivated Emmer, we concluded that the birthplace of cultivated Emmer is Southeastern Turkey. Wild Emmer also originated through hybridization of Ae. speltoides and Triticum urarutu in this area. d. Development of new markers: In order to develop novel molecular markers available for wheat and its relatives, we studied characteristics of SINE sequence that we recently found in Ae. umbellulata. We cloned more than 120 sequences from several species of Triticum and Aegilops. Of these, 30 were allocated to chromosomes of common wheat by nulli-treat analysis. e. Survey of Wild Populations: Kawahara, Mari and Yasui made prelominary field survey in Syria, Turkey and Greece for one month in 2001. During this trip, seeds of wild relatives of wheat were collected in Aegean islands of Greece. In 2002, Mori examined the distribution of wild ancestors of Emmer wheat in Southeastern Turkey.


• Cataloging of sake-brewing rice genetic resources and development of a gene tagging system

  NAKAMURA C, KAMIJIMA O., TAKUMI S., MORI N., ASAKURA W., YOSHIDA S.

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for University and Society Collaboration, Kobe University, 1999 - 2001

  1) Cataloging of sake-brewing rice genetic resources by molecular markers. Sake-brewing rice genetic resources were cataloged based of growth and grain characteristics and AFLP and SSR markers in 253 cultivars (Submitted to Theoretical and Applied Genetics). 2) Genetic and QTL analyzes of the white-core rate (Shinpaku rate) and other sake-brewing characteristics. At least five genes were identified as regulators of the white-core rate using a near-isogenic lines (Breeding Science, in prep.). Using a doubled haploid population derived from a Yamadanishiki x Reihou cross, QTLs related to growth and grain characters of sake-brewing rice including Shinpaku rate were identified and mapped on nee chromosomes (Breeding Science, in press). A similar QTL analysis was conducted to identify QTLs for sake-brewing characterstics using F2 and F3 populations of a Hokuriku42 x Hyogokitanishiki cross. 3) Isolation of novel Tos members and selection of mutant lines/ Using primers for the reverse transcriptase gene of Tos, five novel Tos members of retrotransposons were identified from cultured cells of Hyogoyumenishiki. Through anther culture of gammairradiated young inflorescences of Yamadanishikl several mutant lines were obtained. 4) Isolation and characterization of rice orthologs of maize Mu transposon. Two rice orthologs (OsMuDR) of maize Mu trransposons were isolated by PCR amplification of the previously isolated partial cDNA sequence homologous to the maize MuDR. One clone (OsMu4-1) was an intact sequence with TIRs and TDSs, while another (OsMu10-1) was a deleted version that was likely caused by the interrupted-gap-repair mechanism. OsMu4-1 was proven to have transposed in the past and is still transcriptionally active but a pseudogene. Alternatively spliced transcripts were identified from OsMu4-1. A wide distribution of OsMu elements was shown by Southern blot analysis of wild relatives of rice and data mining of the rice genome. (Molecular Genetics and Genomics, in press).


• Variation in chloroplast microsatellite loci among wild and cultivated species of Emmer wheat

  MORI Naoki, NAKAMURA Chiharu, TAKUMI Shigeo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), KOBE UNIVERSITY, 1999 - 2000

  It is generally accepted that cultivated Emmer wheat (Triticum dicoccum Schubl.) originated from its wild ancestor (T.dicoccoides Korn.) by domestication about eight thousand years ago, somewhere in the Fertile Crescent. To study the origin of the cultivated Emmer wheat, allelic diversity in eight microsatellite loci of the chloroplast genome was investigated using 100 accessions of T.dicoccum and 72 accessions of T.dicoccoides. Five accessions of=T.paleocolchicum Men., two of T.durum L.and one each of T.aestivum L., T.carthlicum Nevski, T.ispahanicum Heslot, T.polonicum L.and T.turgidum L.were analyzed as references. The number of repeats in each locus was determined using PCR followed by PAGE.Polymorphic band patterns were obtained at seven out of the eight loci. As for the polymorphic microsatellites, the number of alleles per locus ranged from two to five with an average of 2.92. Nei's allelic diversity was estimated for each locus within each species. The average allelic diversity of T.dicoccoides (0.349) was about 3.2 times larger than that of T.dicoccum (0.109). One plastotype representing 80 % of the T.dicoccum accession and all accessions of references was identical to that of T.aestivum cv Chinese Spring. Whereas this major plastotype of the cultivated wheat was identified only in six T.dicoccoides accessions collected in one region in the north western Fertile Crescent. In addition to this major maternal lineage, we have identified a minor lineage from the wild Emmer to the cultivated Emmer wheat. These results suggest that there are at least two maternal lineages in cultivated Emmer wheat, and that the domestication of Emmer wheat with the major plastotype occurred in the north western Fertile Crescent.


• Molecular cloning and characterization of cold-responsive genes in wheat and its related species

  NAKAMURA C, TAKUMI S, MORI N

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B)., KOBE UNIVERSITY, 1998 - 2000

  1) Six clones of cold-responsive genes were isolated from a winter-hardy common wheat cultivar 'Mironovska 808', and their structure and expression were studied. a) Wcor14 and Wcs19 : genes gelonging to a Cor (cold-responsive) family. Wcor14 is homologous to cold- and light-responsive barley Bcor14b. Wcs19 has a N-terminal region homologous to that of Wcor14 and Bcor14b. WCOR14, WCS19 and BCOR14b have a 51-amino-acid N-terminal choloroplast leader peptide. b) Wdhn13 : a gene belonging to a hedydrin family. A genomic clone of Wcas19 including a putative promoter was also obtained. c) Wrab 17 : a gene homologous to GA_3-responsive ES2A from a GA_3-insensitive barley mutant. d) Wrab19 : a gene belonging to a group-3 lea gene family. e) Wltr10 : a member of cereal-specific Ltr family. f) Waoxl and Waox2 : genes encoding cyanide-insensitive alternative oxidase. 2) Copy number of each gene was determined by genomic Southern blot analysis. Chromosome assignment was made using nulli-tetrasomic lines and D genome chromosome substiotution lines. Waoxl and Waox2 were shown to be nonhomoeologous genes. 3) Bioassy for freezing tolerance was conducted using 'Mironovska 808' and a spring wheat cv.'Chinese Spring'. Enhancement of freezing tolerance by cold acclimation and significant cultivar differences in the level of freezing tolerance were shown. 4) Gene expression was studied by Northern blot analysis. Expression of Wcor14, Wcs19 and Wltr10 was low temperature-specific. Expression of Wrab17 and Wrab19 was induced by low temperature and also modulated by GA_3, and ABA.Expression of Waox1 and Waox2 was enhanced by low temperature and KCN.A positive correlation was observed between the level of expression of these genes and the level of freezing tolerance in the two cultivars. 5) Transportation of WCOR14 and WCS19 to chloroplasts and that of WAOX1 and WAOX2 to mitochondria was proven by the transient expression assay using GFP-fusion constracts. 6) Genomic clones of Waox1 and Waox2 were isolated and their exson-intron structures were determined. 7) WCOR14 and WDHN13 were synthesized in E.coli and their antibodies were raized using mouse and rabbit.


• Molecular biological study and breeding of cold response in wheat

  NAKAMURA Chiharu, TAKUMI Shigeo, MORI Naoki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B)., KOBE UNIVERSITY, 1998 - 2000

  Japan side(Kobe University) 1)Six clones of cold-responsive genes were isolated from a winter-hardy common wheat cultivar 'Mironovska 808', and their strucutre and expression were studied. a)Wcor14 and Wcs19 : genes gelonging to a Cor(cold-responsive)family. Wcor14 is homologous to cold- and light-responsive barley Bcor14b. Wcs19 has a N-terminal region homologous to that of Wcor14 and Bcor14b. WCOR14, WCS19 and BCOR14b have a 51-amino-acid N-terminal choloroplast leader peptide. b)Wdhn13 : a gene belonging to a hedydrin family. A genomic clone of Wcas19 including a putative promoter was also obtained. c) Wrab17 : a gene homologous to GA_3-responsive ES2A from a GA_3-insensitive barley mutant. d)Wrab19 : a gene belonging to a group-3 lea gene family. e)Wltr10 : a member of cereal-specific Ltr family. f)Waox1 and Waox2 : genes encoding cyanide-insensitive alternative oxidase. 2)Copy number of each gene was determined by genomic Southern blot analysis. Chromosome assignment was made using nulli-tetrasomic lines and D genome chromosome substiotution lines. Waox1 and Waox2 were shown to be nonhomoeologous genes. 3)Bioassy for freezing tolerance was conducted using 'Mironovska 808' and a spring wheat cv.'Chinese Spring'. Enhancement of freezing tolerance by cold acclimation and significant cultivar differences in the level of freezing tolerance were shown. 4)Gene expression was studied by Northern blot analysis. Expression of Wcor14, Wcs19 and Wltr10 was low temperature-specific. Expression of Wrab17 and Wrab19 was induced by low temperature and also modulated by GA_3 and ABA.Expression of Waox1 and Waox2 was enhanced by low temperature and KCN.A positive correlation was observed between the level of expression of these genes and the level of freezing tolerance in the two cultivars. 5)Transportation of WCOR14 and WCS19 to chloroplasts and that of WAOX1 and WAOX2 to mitonchodria was proven by the transient expression assay using GFP-fusion constracts. 6)Genomic clones of Waox1 and Waox2 were isolated and their exson-intron structures were determined. 7)WCOR14 and WDHN13 were synthesized in E.coli and their antibodies were raized using mouse and rabbit. Bulgarian side(Institute of Genetic Enginnering) 1)Bioassay for freezing tolerance was conducted using a large number of tetraploid and hexaploid wheat species and cultivars. 2)Proline systhetase genes were introduced into tobacco plants and the transformants were characterized in their prolione and fructan contents. 3)Near infrared spectrophotometry was applied to monitor cellular changes during freezing/dehydration stresses.


• ECOGENETICAL STUDY ON CROPS IN MEDITERRTANEAN SEA REGION

  FURUTA Yoshihiko, MORI Naoki, OHTA Shoji, MOTIKAWA Toshinobu, TOMINAGA Toru

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for international Scientific Research, Gifu University, 1995 - 1997

  In 1995, three Japanese researchers surveyed in Spain including Canary Islands and Morroco with cooperation of each country. In 1996, four Japanese researchers surveyed in Egypt and Tunisia as scheduled in the proposal. In 1997 all Japanese member visited ten countries serrouding Europian Alps. Party of Furuta, Tominaga and Morikawa field-researched mainly in Chez, Slovakia, Hungary, Slovenia, southern Germany, Austria and Switzerland. They received some conveniences from some professors or researchers in all countries. They collected many spekes and/or seeds of cultivated types of wheat, barley, rye, oat, and their weeds and also green leaves and stems of plants for dry herbalium samples as well as informations in their field and agricultural systems. In addition to them, We got some informations of food processing of these cereals and other utilities. Especially, we collected old type of common wheat, spelta, wheat in southern Germany, Austria and cultivated Einkorn wheat near Chamonix, France. Another parry, Ohta and Mori also surveyedzed mainly in southern Germany, Austria, Switzerland and Italy. They were also assisted by some researchers for their research ineach country and collected about 500 samples. Ohota visited Bari, Italy to get new information.


• タバコの膜結合型オ-キシン受容体と発根遺伝子群

  中村 千春, 森 直樹

  日本学術振興会, 科学研究費助成事業, 重点領域研究, 神戸大学, 1991 - 1991

  1.タバコ膜結合型オ-キシン受容体(ABP)の細胞内所在とサイトカイニンによる活性誘導:Agrobacterium tumefaciens LBA4210(gene4^-)によるタバコの形質転換体を材料に、ABPの細胞内所在とサイトカイニンによるオ-キシン結合活性の誘導について調査した。ABPのオ-キシン結合活性の50%以上が存在した800g画分には、形質膜の標識酵素と液胞膜の標識酵素の活性が認められた。この画分のABPはミクロゾ-ムのABPと同様にオ-キシン結合活性がkinetinにより大幅に増加した。一方、ミクロゾ-ムのABPはTriton Xー100で可容化されたが、この画分のABPは用いた3種の界面活性剤では可容化されなかった。加えて、形質膜のABPとオ-キシンとの結合には他の因子が関与している可能性が示唆された。 2.トウモロコシABPのcDNA塩基配列を利用したPCR法によるABP遺伝子の増幅:トウモロコシで単離されたABPーcDNAの塩基配列を用いてトウモロコシ・パンコムギとタバコのゲノミックDNAからABP遺伝子の増幅を試み、約400塩基からなる主要断片を含む複数の増幅産物を得た。 3.毛状根を利用したタバコ発根遺伝子群のクロ-ニング:Ag.rhizogenes15834によるタバコの毛状根からcDNAバンクを作成し、毛状根組織と根以外の組織から得たcDNAおよびAg.rhizogenes Ri plasmid DNA(TーDNA)を用いた分別スクリ-ニングにより毛状根特異的cDNAのクロ-ンを6種類得た。 4.Agrobacteriumによるタバコの発根変異株の形質転換:Ag.tumefaciensとAg.rhizogenesを用いてタバコの発根変異株2系統(いづれもオ-キシン耐性変異株)の形質転換体の作成を試みた。両変異株ともrhizogenesでは形質転換体が得られず、1系統ではtumefaciensによる形質転換体の全てでテラト-マ状の茎葉分化が認められ、変異株のオ-キシン応答が野生株のそれと異なっていることが示唆された。


• Analysis of cytoplasmic organellar function in wheat and its related species

  NAKAMURA Chiharu, NAKATA Masanobu, MORI Naoki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for General Scientific Research (C), Kobe University, 1989 - 1991

  1. A large variability was found in photosynthetic capacity and respiratory characteristics in nuclar cytoplasmic hybrids of common wheat with cytoplasms of common wheat and its related species. Photosynthetic capacity differed more than 30% among the hybrids, while higer capacity was accompanied by incompatibility, resulting in the reduction of plant vigor of the hydrids. The activities of cytochrome path, cytochrome c oxidase and alternative path differed significantly among the hybrids. The cytochrome c oxidase activity was markedly high in hybrids with cytoplasms causing the reduction of growth vigor. 2. Cytoplasms of two Agropyron species caused marked reduction in growth vigor and male fertilitly when combined with common wheat nucleus, while Agropyron telocentric chromosmes restored normal growth vigor and male fertility. The incompatibility between Agropyron cytoplasms and common wheat nucleus was expressed as aberrant mitochondrial function. 3. Study of chloroplast and mitochondrial DNAs showed that the genetic distances between cytoplasmic genomes of common wheat and the two Agropyron species were nearly the same as that between common wheat and Haynaldia villosa. The cytoplasmic genomes of the two Agropyron species differed significantly. 4. Physical maps of chloroplast DNAs of the two Agropyron species and Haynaldia villosa were constructed and photosynthesis-related genes were located on the maps.