Research activity information

• Dec. 2018 日本農芸化学会, 農芸化学技術賞, 作物の高温耐性を高める揮発性バイオスティミュラント「すずみどり」の開発

  YAMAUCHI YASUO

  Japan society

• Stereospecific reduction of 2′S-configured strigolactones by cowpea OPR3 enzymes

  Shota Suzawa, Misa Yamauchi, Masato Homma, Yasuo Yamauchi, Masaharu Mizutani, Takatoshi Wakabayashi, Yukihiro Sugimoto

  Abstract Strigolactones (SLs), plant-derived apocarotenoids, serve dual roles as phytohormones and rhizosphere signaling molecules. While exogenous administration of SLs to plants aids in studying their functions, the metabolic destiny of these administered SLs remains poorly elucidated. Our previous research demonstrated that among synthetic SL GR24 stereoisomers administered to cowpea (Vigna unguiculata), 2′-epi-GR24 undergoes selective reduction at the C-3′,4′ double bond in its D-ring. In this investigation, we isolated proteins from cowpea roots based on SL reducing activity and identified 12-oxophytodienoate reductase 3 homologs (VuOPR3s) as contributor to this reduction. Enzymatic assays conducted with recombinant proteins revealed that VuOPR3s exhibited a preference for reducing activity toward 2′S-configured SLs, including 2′-epi-GR24. This specificity for 2′S-configured SLs was congruent with that observed for orobanchol produced by cowpea and its stereoisomers. These findings suggest that exogenously administered SLs undergo enzymatic stereoselective reduction, underscoring the importance of considering stereospecificity when interpreting data obtained from SL usage.

  Oxford University Press (OUP), Jul. 2024, Bioscience, Biotechnology, and Biochemistry

  Scientific journal


• Aerial (+)-borneol modulates root morphology, auxin signalling and meristematic activity in Arabidopsis roots

  Kyohei Fukuda, Masayoshi Uefune, Hidehiro Fukaki, Yasuo Yamauchi, Ikuko Hara-Nishimura, Rika Ozawa, Kenji Matsui, Koichi Sugimoto, Kazunori Okada, Ryozo Imai, Kenshi Takahashi, Shinichi Enami, René Wurst, Junji Takabayashi

  One of the characteristic aspects of odour sensing in humans is the activation of olfactory receptors in a slightly different manner in response to different enantiomers. Here, we focused on whether plants showed enantiomer-specific response similar to that in humans. We exposed Arabidopsis seedlings to methanol (control) and (+)- or (−)-borneol, and found that only (+)-borneol reduced the root length. Furthermore, the root-tip width was more increased upon (+)-borneol exposure than upon (−)-borneol exposure. In addition, root-hair formation was observed near the root tip in response to (+)-borneol. Auxin signalling was strongly reduced in the root tip following exposure to (+)-borneol, but was detected following exposure to (−)-borneol and methanol. Similarly, in the root tip, the activity of cyclin B1:1 was detected on exposure to (−)-borneol and methanol, but not on exposure to (+)-borneol, indicating that (+)-borneol inhibits the meristematic activity in the root. These results partially explain the (+)-borneol-specific reduction in the root length of Arabidopsis. Our results indicate the presence of a sensing system specific for (+)-borneol in Arabidopsis.

  The Royal Society, May 2022, Biology Letters, 18(5) (5), English

  [Refereed]

  Scientific journal


• Areal (+)-borneol modulates root morphology, auxin signalling and the promoter activity of cyclin B in Arabidopsis roots

  Kyohei Fukuda, Masayoshi Uefune, Hidehiro Fukaki, Yasuo Yamauchi, Ikuko Nishimura, Rika Ozawa, Kenji Matsui, Koichi Sugimoto, Kazunori Okada, Ryozo Imai, René Wurst, Kenshi Takahashi, Shinichi Enami, Junji Takabayashi

  Apr. 2022, Biology Letters, English

  [Refereed]

  Scientific journal


• CO2 -responsive CCT protein interacts with 14-3-3 proteins and controls the expression of starch synthesis-related genes.

  Hiroshi Fukayama, Fumihiro Miyagawa, Naoki Shibatani, Aiko Koudou, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Yasuo Yamauchi, Daisuke Matsuoka, Ryutaro Morita

  CO2 -responsive CCT protein (CRCT) is a positive regulator of starch synthesis-related genes such as ADP-glucose pyrophosphorylase large subunit 1 and starch branching enzyme I particularly in the leaf sheath of rice (Oryza sativa L.). The promoter GUS analysis revealed that CRCT expressed exclusively in the vascular bundle, whereas starch synthesis-related genes were expressed in different sites such as mesophyll cell and starch storage parenchyma cell. However, the chromatin immunoprecipitation (ChIP) using a FLAG-CRCT overexpression line and subsequent qPCR analyses showed that the 5'-flanking regions of these starch synthesis-related genes tended to be enriched by ChIP, suggesting that CRCT can bind to the promoter regions of these genes. The monomer of CRCT is 34.2 kDa; however, CRCT was detected at 270 kDa via gel filtration chromatography, suggesting that CRCT forms a complex in vivo. Immunoprecipitation and subsequent MS analysis pulled down several 14-3-3-like proteins. A yeast two-hybrid analysis and bimolecular fluorescence complementation assays confirmed the interaction between CRCT and 14-3-3-like proteins. Although there is an inconsistency in the place of expression, this study provides important findings regarding the molecular function of CRCT to control the expression of key starch synthesis-related genes.

  Aug. 2021, Plant, cell & environment, 44(8) (8), 2480 - 2493, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detoxification of reactive carbonyl species by glutathione transferase tau isozymes

  Jun’ichi Mano, Sayaka Kanameda, Rika Kuramitsu, Nagisa Matsuura, Yasuo Yamauchi

  Apr. 2019, Frontiers in Plant Science, 10, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Transcriptome analysis of Arabidopsis thaliana treated with green leaf volatiles: possible role of green leaf volatiles as self-made damage-associated molecular patterns

  YAMAUCHI YASUO, MATSUDA AYA, MATSUURA NAGISA, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  2018, Journal of Pesticide Science, English

  [Refereed]

  Scientific journal


• Chemical Control of Abiotic Stress Tolerance Using Bioactive Compounds

  YAMAUCHI YASUO

  InTech, 2018, Plant, Abiotic Stress and Responses to Climate Change, English

  [Refereed]

  Scientific journal


• Essential role of the PSI-LHCII supercomplex in photosystem acclimation to light and/or heat conditions by state transitions

  Yoko Marutani, Yasuo Yamauchi, Mari Higashiyama, Akihito Miyoshi, Seiji Akimoto, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto

  Jan. 2017, PHOTOSYNTHESIS RESEARCH, 131(1) (1), 41 - 50, English

  [Refereed]

  Scientific journal


• Identification of (Z)-3:(E)-2-Hexenal Isomerases Essential to the Production of the Leaf Aldehyde in Plants

  Mikiko Kunishima, Yasuo Yamauchi, Masaharu Mizutani, Masaki Kuse, Hirosato Takikawa, Yukihiro Sugimoto

  Jul. 2016, JOURNAL OF BIOLOGICAL CHEMISTRY, 291(27) (27), 14023 - 14033, English

  [Refereed]

  Scientific journal


• Identification of (Z)-3:(E)-2-hexenal isomerases essential to the production of the leaf aldehydes in plants

  Mikiko Kunishima, Yasuo Yamauchi, Masaharu Mizutani, Masaki Kuse, Hirosato Takikawa, Yukihiro Sugimoto

  The green odor of plants is characterized by green leaf volatiles (GLVs) composed of C6 compounds. GLVs are biosynthesized from polyunsaturated fatty acids in thylakoid membranes by a series of enzymes. A representative member of GLVs (E)-2-hexenal, known as the leaf aldehyde, has been assumed to be produced by isomerization from (Z)-3-hexenal in the biosynthesis pathway; however, the enzyme has not yet been identified. In this study, we purified the (Z)-3:(E)-2-hexenal isomerase (HI) from paprika fruits and showed that various plant species have homologous HIs. Purified HI is a homotrimeric protein of 110 kDa composed of 35-kDa subunits and shows high activity at acidic and neutral pH values. Phylogenetic analysis showed that HIs belong to the cupin superfamily, and at least three catalytic amino acids (His, Lys, Tyr) are conserved in HIs of various plant species. Enzymatic isomerization of (Z)-3-hexenal in the presence of deuterium oxide resulted in the introduction of deuterium at the C4 position of (E)-2-hexenal, and a suicide substrate 3-hexyn-1-al inhibited HI irreversibly, suggesting that the catalytic mode of HI is a keto-enol tautomerism reaction mode mediated by a catalytic His residue. The gene expression of HIs in Solanaceae plants was enhanced in specific developmental stages and by wounding treatment. Transgenic tomato plants overexpressing paprika HI accumulated (E)-2-hexenal in contrast to wild-type tomato plants mainly accumulating (Z)-3-hexenal, suggesting that HI plays a key role in the production of (E)-2-hexenal in planta.

  Jul. 2016, J. Biol. Chem., 291(27) (27), 14023 - 14033, English, International magazine

  [Refereed]

  Scientific journal


• Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression

  Yasuo Yamauchi, Mikiko Kunishima, Masaharu Mizutani, Yukihiro Sugimoto

  Jan. 2015, SCIENTIFIC REPORTS, 5, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Regulation of Photochemical Energy Transfer Accompanied by Structural Changes in Thylakoid Membranes of Heat-Stressed Wheat

  Yoko Marutani, Yasuo Yamauchi, Akihito Miyoshi, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto

  Dec. 2014, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 15(12) (12), 23042 - 23058, English

  [Refereed]

  Scientific journal


• Gas exchange of root hemi-parasite Striga hermonthica and its host Sorghum bicolor under short-term soil water stress

  T. Inoue, Y. Yamauchi, A. H. Eltayeb, H. Samejima, A. G.T. Babiker, Y. Sugimoto

  Dec. 2013, Biologia Plantarum, 57(4) (4), 773 - 777, English

  [Refereed]

  Scientific journal


• Photosynthetic capacity and stomatal response of root hemi-parasite Striga hermonthica and sorghum under short-term soil water stress

  Inoue, T, YAMAUCHI YASUO, Amani, AE, Samejima, H, Abdel Gabar, EB, Sugimoto, Y

  2013, Biologia Plantarum, English

  [Refereed]

  Scientific journal


• Damage to photosystem II due to heat stress without light-driven electron flow: involvement of enhanced introduction of reducing power into thylakoid membranes

  Marutani Yoko, Yamauchi Yasuo, Kimura Yukihiro, Mizutani Masaharu, Sugimoto Yukihiro

  Aug. 2012, PLANTA, 236(2) (2), 753 - 761

  [Refereed]


• Role of acylamino acid-releasing enzyme/oxidized protein hydrolase in sustaining homeostasis of the cytoplasmic antioxidative system

  Atsushi Nakai, Yasuo Yamauchi, Sawako Sumi, Kiyoshi Tanaka

  Aug. 2012, PLANTA, 236(2) (2), 427 - 436, English

  [Refereed]

  Scientific journal


• Chloroplastic NADPH-dependent alkenal/one oxidoreductase contributes to the detoxification of reactive carbonyls produced under oxidative stress

  Yasuo Yamauchi, Ayaka Hasegawa, Masaharu Mizutani, Yukihiro Sugimoto

  Apr. 2012, FEBS LETTERS, 586(8) (8), 1208 - 1213, English

  [Refereed]

  Scientific journal


• Germination response of Striga hermonthica and Orobanche minor seeds pre-treated with the synthetic strigolactone GR24

  Sawada, R, YAMAUCHI YASUO, Sugimoto, Y

  2012, Recent Research Developments in Phytochemistry, 10, 1 - 12, English

  [Refereed]

  Scientific journal


• Function of extrinsic proteins on stabilization of photosynthetic oxygen-evolving complex

  Yong, L, Kimura, Y, Ohno, T, YAMAUCHI YASUO

  2012, Molecular Photochemistry, 6, 109 - 124, English

  [Refereed]

  Scientific journal


• NADPH-dependent Reductases Involved in the Detoxification of Reactive Carbonyls in Plants

  Yasuo Yamauchi, Ayaka Hasegawa, Ai Taninaka, Masaharu Mizutani, Yukihiro Sugimoto

  Mar. 2011, JOURNAL OF BIOLOGICAL CHEMISTRY, 286(9) (9), 6999 - 7009, English

  [Refereed]

  Scientific journal


• Plants switch photosystem at high temperature to protect photosystem II

  YAMAUCHI YASUO, Marutani, Y, Akimoto, S, Kimura, Y, Mizutani, M, Sugimoto, Y

  2011, Nature Procedings, 6168, English

  Scientific journal


• Photosystem at high temperature: Mechanisms of adaptation and damage. Photochemistry: UV/VIS Spectroscopy

  YAMAUCHI YASUO, Kimura, Y

  2011, UV/VIS Spectroscopy, Photochemical Reactions and Photosynthesis, 203 - 236, English

  [Refereed]

  Scientific journal


• Effect of protein modification by malondialdehyde on the interaction between the oxygen-evolving complex 33 kDa protein and photosystem II core proteins

  Yasuo Yamauchi, Yukihiro Sugimoto

  Apr. 2010, PLANTA, 231(5) (5), 1077 - 1088, English

  [Refereed]

  Scientific journal


• Conversion of Dechlorodauricumine into Miharumine by a Cell-Free Preparation from Cultured Roots of Menispermum dauricum

  Rieko Hori, Gen Sugimoto, Miharu Matsui, Yasuo Yamauchi, Hirosato Takikawa, Yukihiro Sugimoto

  Feb. 2009, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73(2) (2), 440 - 442, English

  [Refereed]

  Scientific journal


• Malondialdehyde generated from peroxidized linolenic acid causes protein modification in heat-stressed plants

  Yasuo Yamauchi, Ai Furutera, Kumiko Seki, Yasuyuki Toyoda, Kiyoshi Tanaka, Yukihiro Sugimoto

  Aug. 2008, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46(8-9) (8-9), 786 - 793, English

  [Refereed]

  Scientific journal


• Marker assisted transfer of stay-green trait of sorghum to enhance terminal drought tolerance: Candidate donor and recipient genotypes

  Abdelbagi, MA, Nasrein, M, Ibrahim, N, Hiraoka, Y, YAMAUCHI YASUO, Sugimoto, Y

  2007, Sudan Journal of Agricultural Research, 10, 133 - 141, English

  [Refereed]

  Scientific journal


• Development of methods for galactinol production using sucrose as a starting material.

  Yasuo Yamauchi

  精糖技術研究会, 2006, Proceedings of the Research Society of Japan Suger Refineriesﾕ Technologists, 54, 37-43, 37 - 44, English

  Scientific journal


• Identification and cloning of a submergence-induced gene OsGGT (glycogenin glucosyltransferase) from rice (Oryza sativa L.) by suppression subtractive hybridization

  YH Qi, N Kawano, Y Yamauchi, JQ Ling, DB Li, K Tanaka

  Jun. 2005, PLANTA, 221(3) (3), 437 - 445, English

  Scientific journal


• The submergence-induced gene OsCTP in rice (Oryza sativa L.) is similar to Escherichia coli cation transport protein ChaC

  YH Qi, Y Yamauchi, JQ Ling, N Kawano, DB Li, K Tanaka

  Jan. 2005, PLANT SCIENCE, 168(1) (1), 15 - 22, English

  Scientific journal


• Cloning of a putative monogalactosyldiacylglycerol synthase gene from rice (Oryza sativa L.) plants and its expression in response to submergence and other stresses

  YH Qi, Y Yamauchi, JQ Ling, N Kawano, DB Li, K Tanaka

  Jul. 2004, PLANTA, 219(3) (3), 450 - 458, English

  Scientific journal


• Over-expression of ascorbate peroxidase in tobacco chloroplasts enhances the tolerance to salt stress and water deficit

  GH Badawi, N Kawano, Y Yamauchi, E Shimada, R Sasaki, A Kubo, K Tanaka

  Jun. 2004, PHYSIOLOGIA PLANTARUM, 121(2) (2), 231 - 238, English

  Scientific journal


• Enhanced tolerance to water deficit and high salt stress by overexpressing superoxide dismutase and ascorbate peroxidase in tobacco chloroplasts

  GH Badawi, Y Yamauchi, N Kawano, K Tanaka, K Tanaka

  2004, PLANT AND CELL PHYSIOLOGY, 45, S230 - S230, English

  Scientific journal


• Identification and biochemical characterization of plant acylamino acid-releasing enzyme

  Y Yamauchi, Y Ejiri, Y Toyoda, K Tanaka

  Aug. 2003, JOURNAL OF BIOCHEMISTRY, 134(2) (2), 251 - 257, English

  Scientific journal


• Blocking ethylene perception enhances flooding tolerance in rice seedlings

  ES Ella, N Kawano, Y Yamauchi, K Tanaka, AM Ismail

  2003, FUNCTIONAL PLANT BIOLOGY, 30(7) (7), 813 - 819, English

  Scientific journal


• Purification of an Aminopeptidase Preferentially Releasing N-terminal Alanine from Cucumber Leaves and Its Identification as a Plant Aminopeptidase N(Biochemistry & Molecular Biology)

  YAMAUCHI Yasuo, EJIRI Yukinori, TANAKA Kiyoshi

  In this study, a highly active foliar aminopeptidase preferentially releasing N-terminal alanine from artificial substrates was purified and characterized from cucumber (Cucumis sativus L. suyo). The enzyme had a molecular mass of 200 kDa consisting of two subunits of 95 kDa. It was a metalloprotease the pH optimum of which was 8 to 9. It cleaved Ala-, Gly-, Met-, Ser-, Leu-, Lys-, and Arg artificial substrates. An internal amino acid sequence was similar to those of aminopeptidase N (clan MA, family Ml) of microorganisms, and was very similar to that of a putative aminopeptidase N of Arabidopsis thaliana. From these results, the highly active aminopeptidase in cucumber leaves was identified to be a plant aminopepitdase N.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Dec. 2001, Bioscience, biotechnology, and biochemistry, 65(12) (12), 2802 - 2805, Russian


• A High Molecular Weight Glutamyl Endopeptidase and Its Endogenous Inhibitors from Cucumber Leaves

  YAMAUCHI Yasuo, EJIRI Yukinori, SUGIMOTO Toshio, SUEYOSHI Kuni, OJI Yoshikiyo, TANAKA Kiyoshi

  We purified a glutamyl endopeptidase that is a major foliar endopeptidase in cucumber. The endopeptidase had a molecular mass of 400 kDa, consisted of four subunits of 97 kDa, and was inactivated by SH-modifying reagents. Its optimum pH and optimum temperature were 8.0 and 30-37°C, respectively. An internal amino acid sequence of the endopeptidase was highly homologous to a partial sequence of unidentified proteins deduced from genetic information for Arabidopsis thaliana, soybean and rice, but not to the sequences of bacterial glutamyl endopeptidases or animal proteases. Therefore, the unidentified proteins might be glutamyl endopeptidases and be widely distributed only among plant species. The activity of the cucumber glutamyl endopeptidase was inhibited by at least three inhibitors existing in cucumber leaves. One of the inhibitors was a competitive inhibitor of 25 kDa, which did not significantly inhibit commercial endopeptidases derived from animals and microorganisms. This suggests that the cucumber glutamyl endopeptidase might be controlled by endogenous inhibitors in vivo.

  Japanese Biochemical Society, Aug. 2001, Journal of Biochemistry, 130(2) (2), 257 - 261, English


• A Method for the Separation and Detection of Proteases Using Substrate-containing Gel Isoelectric Focusing Electrophoresis

  Yamauchi Yasuo, Sugimoto Toshio, Oji Yoshikiyo

  We developed an accurate method to separate and detect proteases using isoelectric focusing electrophoresis. The simultaneous addition of 0.3% octyl-D-glucopylanoside or 8M urea to acrylamide gel containing gelatin and of cytochrome c and pl marker proteins to the sample solution prevented interaction between protease and substrate during electrophoresis. Picogram to nanogram quantities of commercial proteases, papain, chymotrypsin, and proteinase K, were detected at the estimative isoelectric point of these proteases.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Apr. 1995, Bioscience, biotechnology, and biochemistry, 59(4) (4), 767 - 768, English

• New agrochemical technology opened up by green leaf volatile researches

  Yasuo, Yamauchi

  Lead, Mar. 2024, BIOINDUSTRY, 41(3) (3), 37 - 45, Japanese

  [Invited]

  Introduction commerce magazine


• Screening of Volatile Infochemicals Evoking Transient Increase of Cytosolic [Ca²⁺] by Using Arabidopsis Overexpressing GCaMP3

  山内靖雄, 坂本龍哉, 豊田正嗣, 高林純示, 水谷正治, 杉本幸裕

  2023, 日本植物生理学会年会(Web), 64th


• γ-グルタミルトランスペプチダーゼ阻害剤処理がもたらす植物の高温ストレス耐性能の向上

  山内靖雄, 古閑美羽, 水谷正治, 杉本幸裕

  2023, 日本酸化ストレス学会学術集会プログラム・抄録集, 76th


• Identification of proteins involved in the 3-HAC signaling pathway for C. higginsianum resistance in Arabidopsis thaliana

  乾智晴, 井上珠緒, 宮本亜夜, 土佐幸雄, 足助聡一郎, 萩原幹花, 杉本幸裕, 水谷正治, 山内靖雄

  2023, 植物の生長調節, 58(Supplement) (Supplement)


• 新規GGT阻害剤GGsTopがシロイヌナズナの高温ストレス耐性に与える影響

  古閑美羽, 杉本幸裕, 水谷正治, 山内靖雄

  2022, 日本農芸化学会関西支部講演会講演要旨集, 523rd


• Stereoselective reduction of the D-ring in strigolactones

  須澤尚太, 山内美沙, 若林孝俊, 若林孝俊, 若林孝俊, 水谷正治, 山内靖雄, 杉本幸裕, 山内靖雄, 杉本幸裕

  2022, 植物の生長調節, 57(Supplement) (Supplement)


• 植物の香りを農業に活かす : 「植物の言葉」を揮発性バイオスティミュラント

  Corresponding, 2021, 香料, 389, 35 - 40, Japanese

  [Invited]

  Introduction scientific journal


• 「植物の言葉」を応用したバイオスティミュラント『すずみどり』

  Corresponding, Aug. 2020, 農耕と園藝, 75(3) (3), 41 - 44, Japanese

  [Invited]

  Introduction commerce magazine


• 植物の免疫応答に関わるExtra-large G-protein(XLG)の機能解析

  宮本亜夜, 松浦凪沙, 土佐幸雄, 水谷正治, 杉本幸裕, 山内靖雄

  2020, 日本農芸化学会関西支部講演会講演要旨集, 512th


• 「みどりの香り』が植物を高温から守る〜揮発性バイオスティミュラントを活かした新しい農業〜

  YAMAUCHI YASUO

  2019, Aroma Research, 78(2) (2), 12 - 17, Japanese

  [Invited]

  Introduction scientific journal


• Integrated chemical control of abiotic stress tolerance using biostimulants

  YAMAUCHI YASUO

  InTechOpen, Mar. 2018, Plant, Abiotic Stress and Responses to Climate Change, 133 - 144, English

  [Refereed]

  Introduction scientific journal


• 酸化ストレス応答に関わる2-hexenal受容体のスクリーニング

  宮本亜夜, 山内靖雄, 杉本幸裕, 水谷正治

  2018, 日本農芸化学会関西支部講演会講演要旨集, 506th


• シロイヌナズナの7TMおよびカルシウムチャネル欠損株を用いた2-hexenal情報伝達系の解析

  青木仁美, 新出ひかる, 山内靖雄, 水谷正治, 杉本幸裕

  2017, 日本農芸化学会中四国支部講演会講演要旨集(Web), 49th


• シロイヌナズナの2-hexenal情報伝達系に関わるCyclic nucleotide-gated Ca channelのスクリーニング

  新出ひかる, 青木仁美, 山内靖雄, 山内靖雄, 水谷正治, 水谷正治, 杉本幸裕, 杉本幸裕

  2017, 日本農芸化学会関西支部講演会講演要旨集, 501st


• シロイヌナズナの酸化ストレス情報伝達系に関わるシグナル受容体の探索

  東山真理, 青木仁美, 山内靖雄, 中安大, 水谷正治, 杉本幸裕

  2016, 日本農芸化学会関西支部講演会講演要旨集, 497th


• ついに青葉アルデヒドを作る酵素を捕まえた！〜(Z)-3:(E)-2-ヘキセナールイソメラーゼの同定〜

  YAMAUCHI YASUO

  2016, アロマリサーチ, 17, 46 - 47, Japanese

  [Invited]

  Introduction commerce magazine


• P079 Identification and characterization of a novel enzyme, hexenal isomerase in response to wounding in plants

  Kunishima Mikiko, Yamauchi Yasuo, Mizutani Masaharu, Sugimoto Yukihiro

  The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2015, 植物化学調節学会研究発表記録集, 50, 97 - 97, Japanese


• 傷害応答に関わる(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析

  國嶋幹子, 山内靖雄, 水谷正治, 杉本幸裕

  2015, 日本植物生理学会年会要旨集, 56th


• 明所高温条件で誘導されるステート2によるPSII障害回避機構の解析

  丸谷曜子, 山内靖雄, 東山真理, 秋本誠志, 井上加奈子, 池田健一, 水谷正治, 杉本幸裕

  2015, 日本植物生理学会年会要旨集, 56th


• 光・温度条件の変化により形成されるPSI-LHCII超複合体の解析

  丸谷曜子, 山内靖雄, 井上加奈子, 池田健一, 水谷正治, 杉本幸裕

  2014, 日本農芸化学会関西支部講演会講演要旨集, 486th


• 酸化ストレス応答に関わる新たなプレイヤー 〜活性カルボニル〜

  YAMAUCHI YASUO

  Environmental stresses often cause serious damages to plants, and many cases of environmental stresses are accompanied by oxidative stress derived from overproduced reactive oxygen species (ROS). In chloroplasts under oxidative stress, stimulated peroxidation of polyunsaturated fatty acids by ROS generates reactive carbonyls that can act as toxic and signaling chemicals, RSLVs (Reactive Short-chain Leaf Volatiles). In this note, mechanism of production of reactive carbonyls and their physiological roles in environmental stress response are discussed.

  The Japanese Society for Chemical Regulation of Plants, 2014, 植物の生長調節, 49(2) (2), 143 - 148, Japanese

  [Invited]

  Introduction scientific journal


• 高温ストレスを受けた植物において再編成される光化学系分子メカニズムの解析

  丸谷曜子, 山内靖雄, 水谷正治, 杉本幸裕

  2013, 日本植物生理学会年会要旨集, 54th


• シロイヌナズナ由来葉緑体局在Lonプロテアーゼの異種発現と酵素学的解析

  國嶋幹子, 山内靖雄, 水谷正治, 杉本幸裕

  2013, 日本農芸化学会関西支部講演会講演要旨集, 481st


• 59. Gas exchange and stomatal response of root hemi-parasite Striga hermonthica and sorghum under soil water stress

  Inoue Tomoe, Yamauchi Yasuo, Eltayeb Amani Hamad, Samejima Hiroaki, Ueno Kotomi, Babiker Abdel Gabar, Sugimoto Yukihiro

  A pot experiment was conducted to investigate the effects of soil water stress on gas exchange and stomatal response of the upper fully expanded leaf of the root parasitic weed Striga hermonthica and that of its sorghum host. Photosynthetic rate of S. hermonthica was less affected by water stress than sorghum, but the ratio of respiration rate to photosynthetic rate increased considerably under water stress. Thus, under water stress, growth of S. hermonthica may rely more on heterotrophic carbon from host to compensate the low autotrophic carbon gain. Water stress-induced reductions in transpiration rate and stomatal conductance were more severe in infected sorghum than in S. hermonthica. Water stress-induced reduction in stomatal aperture of the leaf was greater in infected sorghum than in S. hermonthica. These results indicated that higher transpiration rate of S. hermonthica under water stress, achieved through greater stomatal aperture, may be related to maintenance of water and solute movement from the host to the parasite.

  The Japanese Society for Chemical Regulation of Plants, 03 Oct. 2011, 植物化学調節学会研究発表記録集, (46) (46), 75 - 75, Japanese


• Stomatal response and photosynthetic capacity of root parasitic weed Striga and sorghum under different water regimes

  INOUE Tomoe, YAMAUCHI Yasuo, BABIKER Abdel Gabar, ELTAYEB Amani Hamad, SAMEJIMA Hiroaki, SUGIMOTO Yukihiro

  30 Mar. 2011, 日本作物學會紀事, 80, 380 - 381, Japanese


• 高温ストレスを受けたコムギにおいてステート遷移により移動するLHCIIタンパク質の解析

  丸谷曜子, 山内靖雄, 秋本誠志, 水谷正治, 杉本幸裕

  2011, 日本農芸化学会関西支部講演会講演要旨集, 472nd


• 80. Purification and molecular analysis of α,β-unsaturated carbonyl-reducing enzymes from cucumber leaves

  Hasegawa Ayaka, Yamauchi Yasuo, Mizutani Masaharu, Sugimoto Yukihiro

  Polyunsaturated fatty acids in biomembranes are oxidized easily, and result in various low-molecular weight compounds. These compounds include α,β-unsaturated carbonyls like acrolein and methylvinylketone, which are highly reactive. The toxicity of these reactive compounds are based on their ability to form the Michael adducts with thiols and amino groups in proteins and nucleic acids. Therefore, it is important to scavenge α,β-unsaturated carbonyls for maintaining homeostasis in plants. In this study, we purifed the acrolein-reducing enzyme from cucumber leaves and determined its partial amino acid sequences. Based on RT-PCR with degenerate primers and RACE strategies, two cDNA sequences contained fully functional region of the enzyme were obtained. One has chloroplast-targeted transit sequence at N-terminal region, and the other has no. These results indicate that cucumber leaves have α,β-unsaturated carbonyl-detoxifying enzymes localizing in chloroplast and cytosol.

  The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2010, 植物化学調節学会研究発表記録集, (45) (45), 97 - 97, Japanese


• 植物における高反応性カルボニル化合物のNADPH依存解毒機構

  山内靖雄, 長谷川綾香, 水谷正治, 杉本幸裕

  2010, 日本農芸化学会関西支部講演会講演要旨集, 467th


• 11. Involvement of ethylene in Striga hermonthica and Orobanche minor seed germination

  Sawada Ryotaro, Yamauchi Yasuo, Sugimoto Yukihiro

  Seeds of root parasitic plants stay dormant until exposed to warm moist environment (conditioning). The conditioned seeds germinate in response to a germination stimulant. Striga seed germination is also induced by ethylene. The expression of ACC synthase and ACC oxidase genes in S. hermonthica was found to be responsive to the stimulant and to conditioning, respectively. We found that pre-treatment with GR24, a synthetic germination stimulant, during conditioning period decreases S. hermonthica seed germination induced by post-treatment with GR24. The present study was undertaken to investigate the mechanisms involved in the decrease of seed responsiveness to the post-treatment. ACC induced S. hermonthica seed germination in a concentration dependent manner, irrespective of GR24 concentrations in the pre-treatment, suggesting that higher concentrations of GR24 induce ACC biosynthesis. This was supported by the facts that higher concentrations of GR24 induced the expression of the ACC synthase gene and ethylene production. The involvement of ethylene in O. minor seed germination was also studied. Neither ACC nor ethephon induced O. minor seed germination. Germination induced by GR24 was not inhibited by 1-methylcyclopropene. Ethylene release from germinating O. minor seeds was not detected. These facts suggest that ethylene is not involved in O. minor seed germination.

  The Japanese Society for Chemical Regulation of Plants, 06 Oct. 2008, 植物化学調節学会研究発表記録集, (43) (43), 25 - 25, Japanese


• Physiological and molecular analyses of effects of malondialdehyde during heat stress in Arabidopsis

  NAKAMOTO Chihiro, HIRAOKA Yukihiro, YAMAUCHI Yasuo, SUGIMOTO Yukihiro

  The Japanese Society for Chemical Regulation of Plants, 04 Oct. 2006, 植物の生長調節 = Regulation of plant growth & development, 41, 59 - 59, Japanese


• Biochemical and molecular biological effects of malondialdehyde formed in heat stressed plants

  Y Yamauchi, K Tanaka, N Wakiuchi, Y Sugimoto

  2006, PLANT AND CELL PHYSIOLOGY, 47, S44 - S44, English

  Summary international conference


• 47. Interactions between the parasitic weed Striga hermonthica and its host Sorghum bicolor at a molecular level

  Hiraoka Yukihiro, Yamauchi Yasuo, Sugimoto Yukihiro

  Striga spp., root parasites of poaceous plants, devastate cereal production in the semiarid Sub Saharan Africa. The life cycle of the parasite is strongly cued to that of its host and is modulated by host-derived chemical signals. To germinate Striga seeds require conditioning in a warm moist environment and exposure to host derived stimulants, collectively named strigolactones. In this study, host-parasite interactions were analyzed at the molecular level. DNA sequences of clones, isolated by a suppression subtractive hybridization strategy from Striga-parasitized sorghum, were homologous to genes for fatty acid desaturase of maize, heat shock protein 82 of rice, calcium dependent protein kinase of maize and sorghum ESTs induced by biotic and abiotic stresses, including wound, pathogen-infection and oxidative stress.

  The Japanese Society for Chemical Regulation of Plants, 13 Oct. 2005, 植物化学調節学会研究発表記録集, (40) (40), 66 - 66, Japanese


• Peroxidation of linolenic acid triggered by heat and reactive oxygen species causes protein modification in chloroplasts

  K Seki, Y Yamauchi, K Tanaka, N Wakiuchi, Y Sugimoto

  2005, PLANT AND CELL PHYSIOLOGY, 46, S78 - S78, English

  Summary international conference


• GLYCATION OF RUBISCO AND ITS INCREASED SUSCEPTIBILITY TO PROTEASES :

  EJIRI Yukinori, YAMAUCHI Yasuo, TANAKA Kiyoshi

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s75, English


• Evaluation of sulfite tolerance of active oxygen scavenging enzymes- and sulfur metabolyzing enzymes-related transgenic plants :

  Sasaki Ryozo, Ashiguchi Atsuhiro, Ishida Yoshinori, Oshima Kenta, Yamauchi Yasuo, Tanaka Kiyoshi

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s124, English


• Detection of submergence stress-induced genes in submergence tolerant rice cultivar by differential display method and expression mechanim of their genes :

  Osako Masaharu, Kawano Naoyoshi, Ito Osamu, Yamauchi Yasuo, Tanaka Kiyoshi

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s114, English


• Detection of high-salt stress-induced genes in high-salt tolerant rice cultivars by differential display method and expression mechanism of their genes :

  Miyata Natsuko, Ito Osamu, Yamauchi Yasuo, Tanaka Kiyoshi

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s115, English


• CLONING OF A GENE ENCODING A PROTEIN HOMOLOGOUS TO CUCUMBER GLUTAMYL ENDOPEPTIDASE FROM ARABIDOPSIS THALIANA :

  YAMAUCHI Yasuo, ITAGAKI Tomoko, TANAKA Kiyoshi

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s204, English


• Regulation of Endopeptidase in Cucumber Leaves-Regulation by endogenous specific inhibitors :

  YAMAUCHI Yasuo, EJIRI Yukinori, TANAKA Kiyoshi

  Japanese Society of Plant Physiologists, 2000, Plant and cell physiology, 41, s166, English


• Comparison of phosphoenolpyruvate carboxylases in seeds of maize (Zea mays) and rice (Oryza sativa)

  Sasai Michitaka, Kamata Toru, Yamauchi Yasuo, Sueyoshi Kuni, Sugimoto Toshio, Oji Yoshikiyo

  Kobe University, Mar. 1999, Memoirs of the Graduate School of Science and Technology, Kobe University. A, 17, 11 - 18, English


• Purification of Two Major Proteases in Cucumber Leaves

  YAMAUCHI Yasuo, ONISHI Michihito, SUGIMOTO Toshio, SUEYOSHI Kuni, OJI Yoshikiyo

  Mar. 1996, Plant and cell physiology, 37, 12 - 12, English


• Emergence of Proteases in Germinating Cucumber Cotyledons and Their Roles in the Two-Step Degradation of Storage Protein :

  Yamauchi Yasuo, Sugimoto Toshio, Sueyoshi Kuni, Oji Yoshikiyo

  The degradation of storage protein in germinating cu-cumber seeds was shown to proceed via two distinct steps. First, several proteases with acidic isoelectric points (pls) were involved in solubilization and partial degradation of 11S globulin. Treatment of seedlings with cycloheximide inhibited this step and the expression of these proteases. Thus, the first step appeared to be governed by these pro-teases, which were synthesized de novo after imbibition. The first step was observed in dark-grown cotyledons, but the complete degradation of 11S globulin did not occur in the absence of illumination. An additional protease, with a pI of 4.5, was induced by illumination, and it was involved in the further cleavage of the partially degraded products of 11S globulin. Thus, the complete degradation of the storage protein proceeded via a two-step process in illuminated germinating seedlings. Light is needed to induce the second step in the degradation of 11S globulin that supplies the nitrogen required for development of the photosynthetic ap-paratus in the greening cotyledon.

  Japanese Society of Plant Physiologists, 1996, Plant and cell physiology, 37(3) (3), 279 - 284, English

• 高温環境下で光化学系活性が低下する２つの異なるメカニズムー障害と応答ー

  Apr. 2022, ISBN: 9784860437695


• 作物の高温耐性を高める揮発性バイオスティミュラント「すずみどり」

  Contributor, Apr. 2022, ISBN: 9784860437695


• バイオスティミュラントハンドブック

  Supervisor, Apr. 2022, ISBN: 9784860430795


• 葉の香りによる作物の高温耐性の向上

  Contributor, 農文協, Jan. 2021, ISBN: 9784540200601


• Function of Extrinsic Proteins in Stabilization of the Photosynthetic Oxygen-Evolving Complex

  Yong Li, Kimura Yukihiro, Ohno Takashi, Yamauchi Yasuo

  Joint work, InTech, 2012, English

  Scholarly book


• Photosystem at high temperature -mechanisms of adaptation and damage

  Yamauchi Yasuo, Kimura Yukihiro

  Joint work, Nova Science Publishers, 2011, English

  Scholarly book

• 植物由来揮発性化合物を用いた収穫後ベビーリーフの鮮度保持効果の検証

  KOBAYASHI Fumi, KUROKI Shinichiro, YAMAUCHI Yasuo, ITOH Hiromichi

  農業環境工学関連5学会2018年合同大会, Sep. 2018, Japanese, 農業環境工学関連5学会, 松山市、愛媛大学, Domestic conference

  Oral presentation


• イネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害する

  水本晃那, 小林亜希子, MATSUOKA DAISUKE, YAMAUCHI YASUO, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  日本光合成学会講演会, 2018, Japanese, Domestic conference

  Poster presentation


• シロイヌナズナの7TMおよびカルシウムチャネル欠損株を用いた2-hexenal情報伝達系の解析

  AOKI HITOMI, SHINDE HIKARU, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本農芸化学会 関西・中四国・西日本支部2017年度合同大阪大会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• Keto-enol tautomerism determining plant leaf fragrance

  YAMAUCHI YASUO

  生物物理学会第55回年会, Sep. 2017, English, Domestic conference

  [Invited]

  Invited oral presentation


• Identification of (Z)-3:(E)-2-hexenal isomerase involved in the production of the leaf aldehyde

  YAMAUCHI YASUO

  第14回日本ナス科コンソーシアム, Sep. 2017, English, 日本大学藤沢キャンパス, International conference

  [Invited]

  Invited oral presentation


• 酸化ストレス応答性シロイヌナズナ GST の解析

  MATSUURA NAGISA, SAKAI HIROKI, YAMAUCHI YASUO, MANO JUN'ICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  農芸化学会関西支部第499回例会, Jun. 2017, Japanese, 京都府立大学, Domestic conference

  Oral presentation


• シロイヌナズナの酸化ストレス情報伝達系に関わるシグナル受容体の探索

  HIGASHIYAMA MARI, AOKI HITOMI, YAMAUCHI YASUO, NAKAYASU MASARU, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本農芸化学会関西支部第497回講演会, Dec. 2016, Japanese, Domestic conference

  Oral presentation


• みどりの香りはストレスシグナルとして機能するか？

  YAMAUCHI YASUO

  植物二次代謝フロンティア研究会, Nov. 2016, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 新規高温耐性誘導剤ヘキセナルを活用したベビーリーフの生産性及び品質向上技術の開発〜ひょうごブランドのベビーリーフ誕生を目指して〜

  YAMAUCHI YASUO

  平成28年度「農」イノベーションひょうご全体交流会, Jun. 2016, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 青葉アルデヒドの生成に重要な役割を果たすヘキセナールイソメラーゼの同定

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本植物生理学会第57回年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• 青葉アルデヒドの生成に関わる酵素遺伝子の同定とトマト香気成分の改変

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本農芸化学会2016年度年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• Manipulation of (2E)-hexenal production in plants to elucidate its roles under biotic and abiotic stress condition

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  Pacifichem 2015, Dec. 2015, English, International conference

  Oral presentation


• Manipulation of (2E)-hexenal production in plants to elucidate its roles under biotic and abiotic stress condition

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  JSBBA KANSAI 2nd Student Forum, Dec. 2015, English, Domestic conference

  Oral presentation


• 明所高温条件で誘導されるステート２によるPSII障害回避機構の解析

  丸谷 曜子, YAMAUCHI YASUO, 東山 真理, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本植物生理学会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 傷害応答に関わる(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本植物生理学会第55回年会, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• 傷害によって誘導される(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本農芸化学会2015年度年会, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• 酸化ストレスシグナル分子として機能する反応性短鎖緑葉揮発性化合物

  YAMAUCHI YASUO, KUNISHIMA MIKIKO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本植物生理学会第56回年会, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• 高温ストレスを受けた植物が明条件でPSII障害を回避するメカニズムの解析

  MARUTANI YOKO, YAMAUCHI YASUO, HIGASHIYAMA MARI, AKIMOTO SEIJI, INOUE KANAKO, IKEDA KEN'ICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本光合成学会若手の会, Dec. 2014, Japanese, Domestic conference

  Oral presentation


• 傷害によって誘導される(3Z):(2E)-ヘキセナールイソメラーゼの同定と酵素学的性質の解明

  KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  第27回植物脂質シンポジウム, Nov. 2014, Japanese, Domestic conference

  Poster presentation


• 光・温度条件の変化により形成されるPSI-LHCII超複合体の解析

  丸谷曜子, YAMAUCHI YASUO, 井上加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本農芸化学会関西支部会, Sep. 2014, Japanese, 奈良先端科学技術大学, Domestic conference

  Oral presentation


• コムギの光化学系における高温耐性機構の解析：ステート遷移の誘導と超高速励起型PSI-LHCII複合体の形成

  丸谷 曜子, YAMAUCHI YASUO, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  第5回日本光合成学会年会, May 2014, Japanese, 近畿大学, Domestic conference

  Poster presentation


• 高温ストレスを受けたコムギにおけるチラコイド膜の構造変化を伴う光化学系の再編成：熱放散とステート遷移の誘導

  丸谷 曜子, YAMAUCHI YASUO, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学, Domestic conference

  Poster presentation


• アグロインフィルトレーション法による植物発現系を用いた植物代謝酵素の機能解析

  KAWAGUCHI DAICHI, YAMAUCHI YASUO, SUGIMOTO YUKIHIRO, MIZUTANI MASAHARU

  第31回植物脂質シンポジウム, Sep. 2013, Japanese, Domestic conference

  Poster presentation


• 高温ストレスを受けた植物において再編成される光化学系分子メカニズムの解析

  MARUTANI YOKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本植物生理学会第54回年会, Mar. 2013, Japanese, Domestic conference

  Poster presentation


• Remodeling of photosystems to induce thermal dissipation and state transition accompanied by structural changes of thylakoid membranes in heat-stressed wheat

  Yoko Marutani, Yasuo Yamauchi, Akimoto S, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto

  The 6th Asia and Oceania Conference on Photobiology, 2013, English, International conference

  Poster presentation


• D1 protein is degraded in heat-stressed plants in the dark, presumably due to electron flow of the stromal-reducing power into the plastoquinone

  MARUTANI YOKO, YAMAUCHI YASUO, KIMURA YUKIHIRO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  10th International Congress on Plant Molecular Biology, Oct. 2012, English, International conference

  Poster presentation


• Isolation of ACC synthase gene from Striga gesnerioides

  RNA Babiker, YAMAUCHI YASUO, SUGIMOTO YUKIHIRO

  International Seminar on SATREPS Project for Striga Management, Sep. 2012, English, International conference

  Oral presentation


• コウモリカズラにおける含塩素イソキノリンアルカノイド生合成中間体の同定

  TAKUMA YUKI, MINAMI MASAKI, NAKASHIMA HITOMI, YAMAUCHI YASUO, MIZUTANI MASAHARU, TAKIKAWA HIROSATO, SUGIMOTO YUKIHIRO

  平成25年度日本植物細胞分子学会, Aug. 2012, Japanese, Domestic conference

  Poster presentation


• Gas exchange and stomatal response of root parasitic weed Striga hermonthica and sorghum under water stress

  INOUE TOMOE, YAMAUCHI YASUO, AMANI HAMAD ELTAYEB, SAMEJIMA HIROAKI, ABDEL GABAR BABIKER, SUGIMOTO YUKIHIRO

  International Society of Root Research, Jun. 2012, English, International conference

  Oral presentation


• 高温ストレスを受けた植物がステート遷移によりPSIIを保護するメカニズムの解析

  MARUTANI YOKO, YAMAUCHI YASUO, AKIMOTO SEIJI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

  日本植物生理学会第53回年会, Mar. 2012, Japanese, Domestic conference

  Poster presentation


• 高温ストレスを受けた植物がステート遷移によりPSIIを保護するメカニズムの解析

  Marutani Yoko, Yamauchi Yasuo, Akimoto Seiji, Kimura Yukihiro, Mizutani Masaharu, Sugimoto Yukihiro

  第53回日本植物生理学会年会, 2012, Japanese, Domestic conference

  Oral presentation


• Translocation of host materials to parasite: Stomatal response and photosynthetic capacity of Striga hermonthica and sorghum under water stress

  T. Inoue, Y. Yamauchi, H. E. Amani, H. Samejima, K. Ueno, A. G. T. Babiker, Y. Sugimoto

  International Seminar on SATREPS Project for Striga Management, 2012, English, Khartoum, Sudan, International conference

  Oral presentation


• 植物が光化学系の高温障害を回避するメカニズムの解析

  Marutani Yoko, Yamauchi Yasuo, Akimoto Seiji, Kimura Yukihiro, Mizutani Masaharu, Sugimoto Yukihiro

  日本農芸化学会関西・中部支部合同大会, 2011, Japanese, Domestic conference

  Oral presentation


• 根寄生植物－宿主植物間相互作用の分子解析（Ⅰ）ストライガの寄生に対するソルガムの応答

  平岡 幸浩, 山内 靖雄, 杉本 幸裕

  植物化学調節学会, 2005, Japanese, 植物化学調節学会, 東京大学, Domestic conference

  Oral presentation


• 高温と活性酸素が引き起こすリノレン酸の過酸化が葉緑体タンパク質の化学修飾をもたらす

  関 久美子, 山内 靖雄, 田中 浄, 脇内 成昭, 杉本 幸裕

  日本植物生理学会年会, 2005, Japanese, 日本植物生理学会, 新潟, Domestic conference

  Oral presentation


• 冠水耐性イネからの新規冠水誘導性遺伝子の単離と発現

  斉 艶華, 山内 靖雄, 凌 建群, 河野 尚由, 李 徳葆, 田中 浄

  日本植物生理学会年会, 2005, Japanese, 日本植物生理学会, 新潟, Domestic conference

  Oral presentation


• Molecular interactions between the parasitic weed Striga hermonthica and its host Sorghum bicolor

  HIRAOKA Yukihiro, MIYAMOTO Sumiyo, YAMAUCHI Yasuo, SUGIMOTO Yukihiro

  Pacifichem2005, 2005, English, 未記入, 未記入, International conference

  Oral presentation


• 植物生体膜構成多価不飽和脂肪酸の過酸化によるタンパク質の化学修飾

  山内 靖雄, 豊田 泰之, 田中 浄

  日本植物生理学会, 2004, Japanese, 日本植物生理学会, 東京都立大学, Domestic conference

  Oral presentation


• ミヤコグサ培養根が生産する根寄生植物種子発芽刺激物質の精製

  上山 知樹, 伊東 ちひろ, 山内 靖雄, 脇内 成昭, 杉本 幸裕

  日本農芸化学会関西支部例会第437回講演会, 2004, Japanese, 日本農芸化学会関西支部, 神戸大学, Domestic conference

  Oral presentation


• コウモリカズラ培養根からのdechlorodauricumineの単離・構造決定

  松井 美春, 滝川 浩郷, 佐々木 満, 山内 靖雄, 脇内 成昭, 杉本 幸裕

  日本農芸化学会関西支部第437回講演会, 2004, Japanese, 日本農芸化学会関西支部, 神戸大学, Domestic conference

  Oral presentation

• Molecular mechanisms of plant redox signaling that are mediated by reactive carbonyl species

  真野 純一, 伊藤 隼哉, 村田 芳行, 梅澤 泰史, 仲川 清隆, 武宮 淳史, 松井 健二, 山内 靖雄

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Yamaguchi University, 01 Apr. 2024 - 31 Mar. 2027


• Sensing of volatile organic compounds by plants: Molecular mechanisms and ecological functions

  高林 純示, 塩尻 かおり, 米谷 衣代, 松井 健二, 山内 靖雄, 村田 純

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kyoto University, 01 Apr. 2022 - 31 Mar. 2027


• 青葉アルデヒド(2-ヘキセナール)の有益昆虫に対する延命効果の可能性検証

  山内 靖雄

  科学技術振興機構, 産学が連携した研究開発成果の展開/大学発新産業創出基金事業/可能性検証, 2023 - 2024

  本研究課題では，植物の高温耐性を向上させる「すずみどり」の主成分である青葉アルデヒド（2-ヘキセナール）が，ミツバチの延命効果を示すための処理時間や処理濃度の特定，分子メカニズムの推定，およびその他の有益昆虫への効果を解析し，青葉アルデヒドを有益昆虫に対する延命剤として応用する可能性を検証する。


• Biochemical mechanism of reactive carbonyl signaling in plants

  真野 純一, 杉本 貢一, 村田 芳行, 深城 英弘, 武宮 淳史, 山内 靖雄

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Yamaguchi University, 01 Apr. 2020 - 31 Mar. 2023

  1) 青色光による気孔開口をRCSが阻害する現象を発見し，青色光シグナル伝達経路におけるRCSの作用点はH+ATPaseの活性化メカニズムの近傍であることを明らかにした。（論文投稿中） 2) ホウレンソウ葉緑体チラコイド膜，包膜に存在するカルボニル種を解析し，包膜には主としてリポキシゲナーゼ反応に由来するC6アルデヒドが存在するのに対し，チラコイド膜にはC3-C11の多様なカルボニル種が含まれており，それらのチラコイド膜内の濃度はmMレベルであることを明らかにした。一方，ストロマに存在するカルボニル種の濃度は極めて低く，ストロマでは可溶性のカルボニル消去物質及び消去酵素によってRCSレベルが極めて低く抑えられていることを明らかにした。（論文投稿中） 3) 植物がもつRCS消去物質として，新たにニンニクからアリインを同定した。アリインはアミノ基にRCSを２分子付加させて消去すること，またそのRCS消去能は，動物由来のRCS消去ペプチドであるカルノシンより高いことを明らかにした。（論文執筆中） 4) RCS消去酵素2-アルケナールレダクターゼ（AER）を葉緑体に局在させる組換えシロイヌナズナを作成した。また，アクロレインを特異的に消去するグルタチオントランスフェラーゼ（GST）アイソザイムTau19（GSTU19）を葉緑体及び細胞質に局在させる組換えシロイヌナズナを作成した。さらにアクロレインと4-ヒドロキシノネナール（HNE）に特異的なGSTU17を葉緑体，細胞質，アポプラストに局在させる組換えシロイヌナズナを作成した。 5) 高塩分が引き起こす酸化ストレスにおいて，アクロレイン，HNE, (E)-2-ペンテナール植物体内で増大し発芽や生長を抑制することを明らかにした。（論文投稿中） 6) 植物の小胞体ストレスに伴いRCSが増大することを明らかにした。（論文投稿中）


• Plant-human communication by plant volatile compounds and its application for prolonging the shelf life of leafy vegetables

  KUROKI Shinichiro

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 28 Jun. 2019 - 31 Mar. 2022

  The effects of artificial exposure to green leaf volatiles (GLVs) on yellowing, quality deterioration, and pathogen resistance of green leaves were investigated. Results showed that trans-2-hexenal has a harmful effect, whereas cis-3-hexenyl acetate has a favorable influence in reducing yellowing and weight loss, and improving resistance against Colletotrichum higginsianum.


• How do plants utilize reactive carbonyl species as specific redox signals?

  MANO Jun'ichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Yamaguchi University, 01 Apr. 2017 - 31 Mar. 2020

  Reactive carbonyl species (RCS), decomposition products of lipid peroxides, mediate oxidative damage and oxidative signal in plants as downstream products of reactive oxygen species (ROS). To elucidate the regulation and function of RCS signal, we examined their metabolism and actions. Following results were obtained. (1) The enzymatic activity of 20 tau isozymes of glutathione transferase (GST) for RCS were examined. A half the tested isozymes scavenged RCS, suggesting that RCS are major endogenous substrates of GST. (2) Addition of ROS or RCS to Arabidopsis thaliana roots induced auxin-responsive genes and induced lateral root (LR). Thus ROS and its downstream products RCS constitute a feed-forward loop of the auxin signal for LR formation. (3) In ROS-triggered programmed cell death (PCD) of tobacco cells, an RCS reductase was found inactivated by ROS at a very early stage. This suggests that the reductase is a ROS sensor to facilitate RCS signal for PCD.


• 稲作に対する根寄生雑草ストライガの脅威の検証と抵抗性・耐性機構の解明

  杉本 幸裕

  科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2019

  Competitive research funding


• 酸化ストレスシグナルの受容機構の解明

  山内 靖雄

  学術研究助成基金助成金／基盤研究(C), Apr. 2015 - Mar. 2018, Principal investigator

  Competitive research funding


• 植物の高温耐性誘導剤の実用化に向けた実証試験

  山内 靖雄

  研究成果展開事業（マッチングプランナー プログラム）, 2016, Principal investigator

  Competitive research funding


• 新規高温耐性誘導剤ヘキセナルを活用したベビーリーフの生産性及び品質向上技術の開発

  山内 靖雄

  兵庫県, 「農」イノベーションひょうご研究開発プロジェクト支援事業, 2015, Principal investigator

  Competitive research funding


• マッチングプランナー「植物の高温耐性誘導剤の実用化に向けた実証試験」

  山内 靖雄

  科学技術振興機構, 研究成果展開事業 マッチングプランナープログラム 探索試験, 2015, Principal investigator

  Competitive research funding


• 高温ストレスによる光化学系障害機構とその光依存獲得性高温耐性機構の解明

  山内 靖雄

  学術研究助成基金助成金／基盤研究(C), 2011, Principal investigator

  Competitive research funding


• 植物独自の光依存性高温耐性機構の遺伝学的解析

  山内 靖雄

  科学研究費補助金／挑戦的萌芽研究, 2009, Principal investigator

  Competitive research funding


• 多機能型高温耐性誘導剤の開発

  山内 靖雄

  科学技術振興機構, 産学が連携した研究開発成果の展開/研究成果展開事業/地域事業/地域イノベーション創出総合支援事業/シーズ発掘試験, 2008 - 2008

  植物を取り巻く環境は悪化の一途を辿っている。特に地球温暖化にともなう気温上昇と熱帯化は、光合成をはじめ植物の様々な機能に悪影響を及ぼし、生育の劣化をもたらしている。そのため、植物に高温耐性をもたらす施策の開発は喫緊の課題である。本研究では、遺伝子組換え技術によらない、植物内在性化学物質の簡便な処理によって植物の高温ストレス耐性能の向上をもたらし、活生育促進剤としての機能を持つ薬剤の開発を目的としている。


• 根寄生植物ストライガの宿主養水分収奪機構の解析

  杉本 幸裕

  科学研究費補助金／基盤研究(B), 2008

  Competitive research funding


• Metabolism of lipid peroxide-derived aldehydes in chloroplasts and their physiological roles in environmental stress

  MANO Jun'ichi, YAMAUCHI Yasuo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Yamaguchi University, 2006 - 2007

  Environmental stresses such as heat, drought and UV-B commonly lead to oxidative damage in leaf cells due to enhanced production of reactive oxygen species in chloroplasts. Recently, lipid peroxide-derived aldehydes have been recognized to mediate cell injury, but their metabolism and toxicology in plants are poorly investigated. In this study we investigated (i) the metabolism of lipid peroxide-derived aldehydes in the chloroplast and (ii) the physiological roles of those aldehydes in the plant responses to environmental stresses. We have obtained the following results : (1) Various species of aldehydes inactivated the CO2-fixation in isolated chloroplasts in vitro. The toxicity was higher for 2-alkenals than n-alkanals. Acrolein and 4-hydroxynonenal showed the greatest toxicity. Several enzymes in the Calvin cycle by 2-alkenals, but the thylakoid electron transport chain was much less affected. (2) Glutathione supplemented to chloroplasts retarded the aldehyde-induced loss of CO_2-fixation, but ascorbate did not, showing the specific role of glutathione to scavenge aldehydes. Aldehydes were extracted from distinct compartments in the chloroplast, derivatized with 2,4-dinitrophenylhydrazine and determined with HPLC. Lipoxygenase pathway-dependent C6 aldehydes were major components found in envelopes and stroma. Thylakoids contained greater variety of aldehydes, including highly toxic 2-alkenals and 4-hydroxy-2-alkenals, suggesting non-enzymatic(reactive oxygen species-mediated)production of aldehydes in thylakoids. (3) Aldehyde composition in the linolenic acid-deficient Arabidopsis mutant was compared with that in wild type. Acrolein, propionaldehyde malondialdehyde and 2E-pentenal were derived from linolenic acid. (4) When Arabidopsis plants were heat stressed or tobacco leaves were strongly illuminated, aldehyde-modification of several chloroplast enzymes in them were increased. One of the malondialdehyde-modified proteins was OEC33 protein, suggesting the production of malondialdehyde in the lumen. Thus heat- or light-stress induces the production highly toxic aldehydes from lipid peroxides in chloroplasts, leading to inactivation of the Calvin cycle. Glutathione is a critically important detoxicant against aldehydes.


• 根寄生植物ストライガの繁殖を起定する宿主植物ソルガムの形質および環境要因の解析

  杉本 幸裕

  科学研究費補助金／基盤研究(B), 2005

  Competitive research funding


• 寄生成立過程における宿主植物―根寄生植物間相互作用の分子解析

  杉本 幸裕

  科学研究費補助金／基盤研究(C), 2005

  Competitive research funding


• アクチベーションタギング法による環境ストレス耐性植物の作製と原因遺伝子の単離

  山内 靖雄, 田中 浄

  日本学術振興会, 科学研究費助成事業, 萌芽的研究, 鳥取大学, 1998 - 1999

  本研究では、強力な遺伝子転写プロモーターを植物に人為的に導入し、無作為に種々の植物ゲノム遺伝子の転写を活性化させることにより、さまざまな転写活性型突然変異体を作製し、原因となっている転写活性化された遺伝子のクローニングをするアクチベーションタギング法を用いて、環境ストレス耐性に関与する遺伝子をクローニングすることを最終目的として実験を行った。 実験材料は当初はタバコとシロイヌナズナを用いて行ったが、シロイヌナズナを用いる形質転換係が芳しくなく、効率的に変異体を作製することができなかったので、当研究室で確立しているタバコを用いた形質転換系を用いて変異体植物作製を集中的に行った。 本実験ではアグロバクテリウム(GV3101)とアクチベーションタギング用プラスミド(pPCVICEn4HPT)を組み合わせ、タバコ葉片に感染法によりプロモーターを導入した。プロモーター導入後のタバコカルスを抗生物質(ハイグロマイシン)を含んだ培地で選抜し、変異体植物タバコを完全体に再生した。第一世代の形質転換体タバコは種子を得るため、約6ヶ月間種子が完熟するまで栽培した。その結果、計191系統の形質転換体タバコを得ることができた。現在、得られた突然変異体タバコを播種し、塩ストレス、乾燥ストレスを負荷し、ストレスに耐性を持った個体を選抜中である。また、これらの形質転換体タバコのうちいくつかは、形態的に野生株と異なっているものがあり、今後はこのようなタバコ個体の遺伝子解析も行う予定である。


• CLARIFICATION OF MECHANISM OF SUBMERGENCE-TOLERANCE USING A SUBMERGENCE-TOLERANT RICE CULTIVAR

  TANAKA Kiyoshi, YAMAUCHI Yasuo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), TOTTORI UNIVERSITY, 1998 - 1999

  The visual damage of submergence usually develops after desubmergence. A part of the damage may be caused by active oxygen produced in transition of oxygen environment. The submergence-intolerant rice cultivar, IR42 suffered from more lipid peroxidation with the production of HィイD22ィエD2OィイD22ィエD2 than submergence-tolerant cultivar, FR13A, after desubmergence. The activities of active oxygen scavenging enzymes in both cultivars were about the same or higher relative to the nonsubmerged control. After 6d of submergence, the total ascorbate levels in both cultivars decreased by 81% and 55% for IR42 and FR13A, respectively. During 7d of recovery after desubmergence, however, FR13A increased level of total ascorbate up to 150% while IR42 did it up to only 78%. Moreover, the proportion of ascorbic acid to total ascorbate I FR13A increased significantly after 3d of recovery and reached the level of that in nonsubmerged control, but the proportion in IR42 was still low during the recovery phase. It means that FR13A could operate the AOS scavenging system better than IR42 after desubmergence. It is suggested that the AOS may be one of the causes of the damage after desubmergence and ascorbate may take ana active role in alleviating the damage. Three cDNA fragments were isolated by differential screening between FR13A desubmerged for 2days after seven days' submergence was cancelled, and non-submerged control FR13A. The one was a giberellin-synthesis-related gene, the second was a proteasome-related gene, and the third was a tolerant gene to pathogenic microorganisms.