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OKADA KeisukeUniversity Hospital / UrologyAssociate Professor
Research activity information
■ Paper- (一社)日本生殖医学会, Nov. 2020, 日本生殖医学会雑誌, 65(4) (4), 307 - 307, Japanese無精子症に対する精索静脈瘤手術の治療成績
- (一社)日本生殖医学会, Nov. 2020, 日本生殖医学会雑誌, 65(4) (4), 338 - 338, Japanese当院におけるOnco-TESEの検討
- (一社)日本生殖医学会, Nov. 2020, 日本生殖医学会雑誌, 65(4) (4), 387 - 387, Japanese男性低ゴナドトロピン性性腺機能低下症へのゴナドトロピン補充療法中、補充中止可能と判断した3症例
- (一社)日本生殖医学会, Nov. 2020, 日本生殖医学会雑誌, 65(4) (4), 390 - 390, Japanese特発性造精機能障害に対するレトロゾールの治療効果の検討
- (一社)日本内分泌学会, Feb. 2020, 日本内分泌学会雑誌, 95(4) (4), 1559 - 1559, Japanese
- Feb. 2020, Andrologia, 52(1) (1), EnglishSperm retrieval from a male with the rare 47, XXYqs variant of Klinefelter syndrome for intracytoplasmic sperm injection: A case report[Refereed]
- (一社)日本生殖医学会, Oct. 2019, 日本生殖医学会雑誌, 64(4) (4), 386 - 386, Japanese精巣精子採取術後の合併症の検討
- (一社)日本生殖医学会, Jul. 2019, 日本生殖医学会雑誌, 64(3) (3), 105 - 105, Japanese低ゴナドトロピン性性腺機能低下症に対しゴナドトロピン補充療法を中断後、再開を余儀なくされた1例
- 泌尿器科紀要刊行会, Jun. 2019, 泌尿器科紀要, 65(6) (6), 252 - 252, Japaneseラットセルトリ細胞のミラベグロンによる分子細胞生物学的影響の検討
- Mirabegron is a selective beta3-adrenoceptor (β3 -AR) agonist, which is commonly used for the treatment of overactive bladder. This medicine is associated with atrophy of reproductive organs in rats. However, no study has examined the detailed action and mechanism of its toxicity in reproductive cells. In this study, we examined the effect of mirabegron on primary cultured rat Sertoli cells. Firstly, RT-PCR and immunocytochemistry revealed that β3 -AR was present in rat Sertoli cells. Then, primary cultured rat Sertoli cells were treated with mirabegron. Quantitative real-time PCR revealed that mirabegron treatment induced a significant increase in claudin-11 mRNA, which is crucial for spermatogenesis. Western blot analysis also showed that mirabegron treatment significantly activated p44/42 mitogen-activated protein kinase (MAPK). After additional treatment with U0126, a specific noncompetitive inhibitor of mitogen-activated protein kinase kinase (MAPKK), the upregulation of claudin-11 mRNA induced by mirabegron was reduced. At the same time, immunocytochemistry showed mirabegron treatment disturbed claudin-11 localisation to tight junction, which was recovered when treated with mirabegron in the presence of U0126. These results suggest that mirabegron treatment is associated with assembly of the blood-testis barrier through p44/42 MAPK pathway. These findings could explain one of the underlying mechanisms of reproductive toxicity induced by mirabegron.Jun. 2019, Andrologia, 51(5) (5), e13241, English, International magazine[Refereed]Scientific journal
- The survival rates of boys and men with cancer have increased due to advances in cancer treatments; however, maintenance of quality of life, including fertility preservation, remains a major issue. Fertile male patients who receive radiation and/or chemotherapy face temporary, long-term, or permanent gonadal damage, particularly with exposure to alkylating agents and whole-body irradiation, which sometimes induce critical germ cell damage. These cytotoxic treatments have a significant impact on a patient's ability to have their own biological offspring, which is of particular concern to cancer patients of reproductive age. Therefore, various strategies are needed in order to preserve male fertility. Sperm cryopreservation is an effective method for preserving spermatozoa. Advances have also been achieved in pre-pubertal germ cell storage and research to generate differentiated male germ cells from various types of stem cells, including embryonic stem cells, induced pluripotent stem cells, and spermatogonial stem cells. These approaches offer hope to many patients in whom germ cell loss is associated with sterility, but are still experimental and preliminary. This review examines the current understanding of the effects of chemotherapy and radiation on male fertility.Lead, May 2019, The world journal of men's health, 37(2) (2), 166 - 174, English, International magazine[Refereed]
- (一社)日本泌尿器科学会総会事務局, Apr. 2019, 日本泌尿器科学会総会, 107回, PP1 - 311, Japanese低ゴナドトロピン性性腺機能低下症39例の臨床的検討
- (一社)日本泌尿器科学会総会事務局, Apr. 2019, 日本泌尿器科学会総会, 107回, PP1 - 316, Japanese非閉塞性無精子症患者の染色体異常に関する臨床的検討
- This study aimed to investigate the effects of testosterone replacement therapy (TRT) on lower urinary tract symptoms (LUTS) in men with late-onset hypogonadism (LOH) and to identify parameters predicting the efficacy of TRT in improving LUTS. This study included 60 consecutive Japanese men who were diagnosed with LOH and subsequently received TRT between January 2009 and December 2014. In this series, 250 mg of testosterone was injected intramuscularly every 3 or 4 weeks in all patients. The following parameters were retrospectively reviewed: body mass index (BMI), Aging Male Symptom (AMS) score, International Prostate Symptom Score (IPSS), International Index of Erectile Function-5 (IIEF-5) score, residual urine volume, prostate volume, serum levels of the prostate-specific antigen (PSA), and total- and free-testosterone levels before and 6 months after TRT. No significant differences were observed in BMI, residual urine volume, or prostate volume between surveys before and after TRT. The AMS score, IPSS, and IIEF-5 score were significantly improved and significant increases were noted in the serum levels of PSA and total- and free-testosterone levels after TRT. An analysis of IPSS subscores documented the significant improvement in storage symptom scores, but not in voiding symptom scores after TRT. Multivariate analyses of parameters assessed in this study identified the pretreatment AMS score, posttreatment IIEF-5 score, and prostate volume as independent predictors of improvements in IPSS following TRT. This study revealed that TRT appeared to have considerable therapeutic effects on LUTS, particularly on storage symptoms, in men with LOH.Lead, Sep. 2018, American journal of men's health, 12(5) (5), 1403 - 1408, English, International magazine[Refereed]Scientific journal
- (一社)日本生殖医学会, Aug. 2018, 日本生殖医学会雑誌, 63(3) (3), 289 - 289, Japanese低ゴナドトロピン性性腺機能低下症39例の臨床的検討
- (一社)日本生殖医学会, Aug. 2018, 日本生殖医学会雑誌, 63(3) (3), 383 - 383, Japanese摂食障害による男性低ゴナドトロピン性性腺機能低下症の1例
- (一社)日本生殖医学会, Aug. 2018, 日本生殖医学会雑誌, 63(3) (3), 423 - 423, Japanese男性不妊に対するクエン酸クロミフェンの効果
- (一社)日本生殖医学会, Apr. 2018, 日本生殖医学会雑誌, 63(1-2) (1-2), 119 - 119, Japanese過度なダイエットによりED、射精障害を認めた1例
- (一社)日本泌尿器科学会総会事務局, Apr. 2018, 日本泌尿器科学会総会, 106回, PP3 - 112, Japanese顕微鏡下精巣精子採取術(micro TESE)の治療成績
- (一社)日本泌尿器科学会総会事務局, Apr. 2018, 日本泌尿器科学会総会, 106回, PP3 - 229, JapaneseSUMO化阻害によるセルトリ細胞の核受容体やsignaling pathwayへの影響
- (一社)日本泌尿器科学会総会事務局, Apr. 2018, 日本泌尿器科学会総会, 106回, PP3 - 374, Japanese前立腺肥大を有さないLOH症候群患者の血清テストステロン値と夜間頻尿との関係
- (一社)日本生殖医学会, Oct. 2017, 日本生殖医学会雑誌, 62(4) (4), 445 - 445, Japaneseクラインフェルター症候群以外の染色体異常を有する非閉塞性無精子症患者の臨床的検討
- (一社)日本生殖医学会, Oct. 2017, 日本生殖医学会雑誌, 62(4) (4), 457 - 457, JapaneseSUMO化阻害によるセルトリ細胞のsignaling pathwayへの影響
- (一社)日本生殖医学会, Oct. 2017, 日本生殖医学会雑誌, 62(4) (4), 508 - 508, Japanese非閉塞性無精子症に対してサルベージ内分泌療法を施行した2例
- 泌尿器科紀要刊行会, Jul. 2017, 泌尿器科紀要, 63(7) (7), 290 - 291, Japanese造精機能障害および精巣Adrenal rest tumorを伴った先天性副腎皮質過形成の2例
- We evaluated the effects of dutasteride on the genitourinary tract using fifteen 8-week-old male Sprague-Dawley rats. Animals were divided into three groups comprising five animals each and treated as follows. Group A was a control group, members of Group B received oral administration of dutasteride 0.1 mg/kg/day from the age of 8 to 16 weeks, and members of Group C were castrated at the age of 8 weeks. All rats were killed at the age of 16 weeks for the sample collection of blood, bladder, prostate, seminal vesicles, and penis. Then, we evaluated the pathological examination for evaluating the tissue fibrosis and hormonal receptor expression. The results showed that the mean size of the prostate and seminal vesicles was smaller in Group B and Group C than in Group A. Serum and tissue concentrations of both testosterone and dihydrotestosterone were remarkably reduced in serum and all tissues in Group C compared with Group A. On the other hand, in Group B, only dihydrotestosterone was reduced in serum and penis. Histopathological examination revealed that Group C showed statistically significant histological changes, such as an increase in fibrotic tissue in the bladder, prostate, and penis. Similarly, Group B showed fibrotic changes in the prostate and penis compared with the Group A. Immunofluorescent staining revealed that the androgen receptor was more strongly expressed than the estrogen receptor beta in Group A. On the other hand, in Group C, weak expression of the androgen receptor and strong expression of the estrogen receptor beta was noted. In Group B, these changes were noted in the prostate and penis. These findings suggest that dutasteride cause morphological changes not only in prostate but also in penis. These changes are associated with altered expression patterns of androgen receptor and estrogen receptor.WILEY, Mar. 2017, ANDROLOGY, 5(2) (2), 347 - 353, English[Refereed]Scientific journal
- (一社)日本生殖医学会, Oct. 2016, 日本生殖医学会雑誌, 61(4) (4), 448 - 448, Japanese造精機能障害および精巣adrenal rest tumorを伴った先天性副腎皮質過形成の2例
- (一社)日本生殖医学会, Oct. 2014, 日本生殖医学会雑誌, 59(4) (4), 283 - 283, Japanese配偶子形成メカニズムの解明 精子形成とCell Junction
- Oct. 2014, ANDROLOGIA, 46(8) (8), 948 - 950, English[Refereed]Scientific journal
- Lead, Jun. 2014, AGING MALE, 17(2) (2), 72 - 75, English[Refereed]Scientific journal
- Lead, Feb. 2014, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 445(1) (1), 89 - 94, English[Refereed]Scientific journal
- (一社)日本生殖医学会, Oct. 2013, 日本生殖医学会雑誌, 58(4) (4), 371 - 371, JapaneseRA-GEF-2(Rapgef6)のマウス精子形成における役割
- Jun. 2013, 日本抗加齢医学会総会プログラム・抄録集13回, 228, Japanese総テストステロン値別による加齢男性性腺機能低下症候群患者の治療効果の検討Research society
- (一社)日本泌尿器科学会, Mar. 2013, 日本泌尿器科学会雑誌, 104(2) (2), 474 - 474, Japaneseマウス精子形成におけるRA-GEF-2の機能解析
- Mar. 2013, BIOLOGY OF REPRODUCTION, 88(3) (3), English[Refereed]Scientific journal
- (一社)日本生殖医学会, Oct. 2012, 日本生殖医学会雑誌, 57(4) (4), 327 - 327, Japaneseマウス精子形成におけるRA-GEF-2のノックアウトマウスを用いた機能解析
- (一社)日本生殖医学会, Oct. 2012, 日本生殖医学会雑誌, 57(4) (4), 440 - 440, Japanese精巣adrenal rest tumorを伴った21水酸化酵素欠損症に合併した乏精子症の一例
- Oct. 2012, SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, 58(5) (5), 274 - 277, English[Refereed]Scientific journal
- (一社)日本内分泌学会, Sep. 2012, 日本内分泌学会雑誌, 88(2) (2), 735 - 735, Japanese
- Jun. 2012, UROLOGY, 79(6) (6), 1340 - 1345, English[Refereed]Scientific journal
- Feb. 2012, INTERNATIONAL JOURNAL OF UROLOGY, 19(2) (2), 149 - 154, English[Refereed]Scientific journal
- 日本腎泌尿器疾患予防医学研究会, Jul. 2019, 日本腎泌尿器疾患予防医学研究会プログラム・抄録集, 28回, 34 - 34, Japanese当院におけるUS-MRI fusion biopsyの初期経験
- 泌尿器科紀要刊行会, Apr. 2019, 泌尿器科紀要, 65(4) (4), 134 - 134, Japanese妊娠高血圧を契機に褐色細胞腫と診断されたMixed cortico-medullary tumor(MCMT)の1例
- 泌尿器科紀要刊行会, Mar. 2019, 泌尿器科紀要, 65(3) (3), 93 - 93, Japanese関節リウマチ、原発性胆汁性肝硬変を合併した腎移植患者に発症した腎移植後リンパ増殖性疾患の1例
- 泌尿器科紀要刊行会, 31 Jul. 2018, 泌尿器科紀要, 64(7) (7), 314 - 314, Japanese術前診断で副腎悪性腫瘍が疑われた副腎Black Adenomaの1例
- 日本内分泌外科学会・日本甲状腺外科学会, May 2018, 日本泌尿器科学会総会(Web), 106th(Suppl.1) (Suppl.1), ROMBUNNO.PP2‐111 (WEB ONLY) - S138, Japanese当院における副腎褐色細胞腫に対する腹腔鏡下手術の臨床的検討
- (一社)日本泌尿器科学会総会事務局, Apr. 2018, 日本泌尿器科学会総会, 106回, PP2 - 111, Japanese当院における副腎褐色細胞腫に対する腹腔鏡下手術の臨床的検討
- Apr. 2014, JOURNAL OF UROLOGY, 191(4) (4), E741 - E741, EnglishLOSS OF RA-GEF-2 (RAPGEF6) IN MOUSE CAUSES ALTERED LOCALIZATION OF N-CADHERIN AND CAN CAUSE MALE INFERTILITYSummary international conference
- Apr. 2014, JOURNAL OF UROLOGY, 191(4) (4), E743 - E743, EnglishNEPHRIN-PODOCIN COMPLEX PLAY AN IMPORTANT ROLE IN SPERMATOGENESISSummary international conference
- Jun. 2013, JOURNAL OF SEXUAL MEDICINE, 10, 230 - 230, EnglishCOMPREHENSIVE EVALUATION OF ANDROGEN REPLACEMENT THERAPY FOR MEN WITH LATE-ONSET HYPOGONADISMSummary international conference
- Apr. 2013, JOURNAL OF UROLOGY, 189(4) (4), E839 - E839, EnglishCLUSTERIN HAS A POSSIBILITY TO PROTECT THE INJURY OF TESTICULAR CELLS INDUCED BY HEAT SHOCK STRESSSummary international conference
- Sep. 2012, UROLOGY, 80(3) (3), S244 - S244, EnglishClinical Assessment of Microdissection Testicular Sperm Extraction in 307 Patients with Non-obstructive AzoospermiaSummary international conference
- Feb. 2012, EUROPEAN UROLOGY SUPPLEMENTS, 11(1) (1), E59 - U404, EnglishSignificant improving of sperm DNA quality after microsurgical repair of varicoceleSummary international conference
- Feb. 2012, EUROPEAN UROLOGY SUPPLEMENTS, 11(1) (1), E51 - U389, EnglishExpression pattern of testicular claudin-11 in infertile menSummary international conference
- 第36回泌尿器科漢方研究会, May 2019男性不妊症に対する漢方治療の検討
- 第107回 日本泌尿器科学会総会, Apr. 2019, Japanese男性不妊に対するクエン酸クロミフェンの治療効果の検討
- APSSM Annual meeting 2019, Apr. 2019, EnglishEffect of clomiphene citrate therapy for male infertility
- 第28回 泌尿器科分子・細胞研究会, Feb. 2019, Japanese, 下関, Domestic conferenceラットセルトリ細胞のミラベグロンによる分子細胞生物学的影響の検討Oral presentation
- 第240回 日本泌尿器科学会関西地方会, Feb. 2019, Japaneseサブクリニカルクッシング症候群を合併した特発性アルドステロン症の1例
- 第68回日本泌尿器科学会中部総会, Oct. 2018, Japanese, 名古屋, Domestic conferenceラットセルトリ細胞のミラベグロンによる分子細胞生物学的影響の検討Poster presentation
- 第68回日本泌尿器科学会中部総会, Oct. 2018, Japanese, 名古屋, Domestic conference5-アミノレブリン酸を用いた経尿道的膀胱腫瘍切除術16例の初期経験Oral presentation
- 第29回 日本性機能学会学術総会, Sep. 2018, Japanese, 神戸, Domestic conference当院での持続勃起症治療に関する検討Poster presentation
- 第29回 日本性機能学会学術総会, Sep. 2018, Japanese, 神戸, Domestic conferenceLOH症候群患者に対する男性ホルモン補充療法の性機能への影響と治療満足度との関連Poster presentation
- 第37回 日本アンドロロジー学会, Jun. 2018, Japanese, 神戸, Domestic conference顕微鏡下精巣精子採取術(microTESE)の治療成績Poster presentation
- 第37回 日本アンドロロジー学会, Jun. 2018, Japanese, 神戸, Domestic conferenceミラベグロンによるラットセルトリ細胞に対する分子機構変化の検討Poster presentation
- 第37回 日本アンドロロジー学会, Jun. 2018, Japanese, 神戸, Domestic conferenceLOH症候群患者に対する男性ホルモン補充療法の性機能への影響と治療満足度との関連Poster presentation
- The 113th American Urological Association, May 2018, English, San Francisco, International conferenceThe usage rate of semen cryopreservation before cancer treatmentPoster presentation
- The 113th American Urological Association, May 2018, English, San Francisco, International conferenceMolecular changes induced by mirabegron in rat Sertoli cell primary culturePoster presentation
- 第106回日本泌尿器科学会総会, Apr. 2018, Japanese, 京都, Domestic conference射精障害に対するアモキサピンおよびイミプラミンの有用性Poster presentation
- 第106回日本泌尿器科学会総会, Apr. 2018, Japanese, 京都, Domestic conference顕微鏡下精巣精子採取術(microTESE)の治療成績Poster presentation
- 第106回日本泌尿器科学会総会, Apr. 2018, Japanese, 京都, Domestic conferenceSUMO化阻害によるセルトリ細胞のsignaling pathwayへの影響Poster presentation
- 第106回日本泌尿器科学会総会 16th Urological Association of Asia (UAA) Congress 2018, Apr. 2018, English, 京都, International conferenceMolecular changes induced by mirabegron in rat Sertoli cell primary culturePoster presentation
- 第237回 日本泌尿器科学会関西地方会, Feb. 2018, Japanese, 日本泌尿器科学会, 兵庫, Domestic conference関節リウマチ、原発性胆汁性肝硬変を合併した腎移植患者に発症した腎移植後リンパ増殖性疾患の1例Oral presentation
- 第50回 関西アンドロロジーカンファレンス, Feb. 2018, Japanese, 関西アンドロロジーカンファレンス, 大阪, Domestic conference過度なダイエットによりED、射精障害を認めた1例Oral presentation
- 第30回 日本内視鏡外科学会総会, Dec. 2017, Japanese, 日本内視鏡外科学会, 京都, Domestic conference副腎褐色細胞腫に対する腹腔鏡下手術の臨床的検討Poster presentation
- 第62回 日本生殖医学会学術講演会, Nov. 2017, Japanese, 日本生殖医学会, 山口, Domestic conference非閉塞性無精子症に対してサルベージ内分泌療法を施行した2例Poster presentation
- 第17回 日本Men's Health医学会, Oct. 2017, Japanese, 日本Men's Health医学会, 東京, Domestic conference前立腺肥大を有さないLOH症候群患者の血清テストステロン値と夜間頻尿との関係Oral presentation
- 第236回 日本泌尿器科学会関西地方会, Oct. 2017, Japanese, 日本泌尿器科学会, 神戸, Domestic conference術前診断で副腎悪性腫瘍が疑われた副腎Black adenomaの1例Oral presentation