Research activity information

• Tracking Particle‐Encapsulated DNA Across the Anion‐Exchange Chromatography Fractions of Recombinant Adeno‐Associated Virus Using Droplet Digital PCR and High‐Throughput Sequencing

  Noriko Hashiba, Yuzhe Yuan, Emi Itou‐Kudo, Kyoko Masumi‐Koizumi, Keisuke Yusa, Kazuhisa Uchida

  Jun. 2025, Biotechnology Journal


• Encapsulation of a Single-Stranded Form of DNA Impurities into the Capsid of a Recombinant Adeno-Associated Virus

  Kazuhisa Uchida, Emi Ito-Kudo, Kiyoko Higashiyama, Kyoko Masumi-Koizumi, Keisuke Yusa, Yuzhe Yuan

  May 2025, Human Gene Therapy


• Database Chemistry for Genomics-Based Safety and Quality Evaluation of Biologics

  Norichika Ogata, Tomoko Matsuda, Aoi Hosaka, Akihisa Shina, Noriko Hashiba, Kazuhisa Uchida, Yoshinori Kawabe, Masamichi Kamihira, Takanori Yamaoka, Hiromu Kurita, Noriko Yamano-Adachi, Takeshi Omasa

  Feb. 2025


• Concise Analysis of Single-Stranded DNA of Recombinant Adeno-Associated Virus By Automated Electrophoresis System

  Yuzhe Yuan, Kiyoko Higashiyama, Noriko Hashiba, Kyoko Masumi-Koizumi, Keisuke Yusa, Kazuhisa Uchida

  Feb. 2024, Human Gene Therapy


• Evaluation of next-generation sequencing performance for in vitro detection of viruses in biological products

  Takamasa Hirai, Kiyoko Kataoka, Yuzhe Yuan, Keisuke Yusa, Yoji Sato, Kazuhisa Uchida, Ken Kono

  Feb. 2024, Biologicals


• Association between adeno‐associated virus genomic titers and intracellular plasmid levels

  Kyoko Masumi‐Koizumi, Yuzhe Yuan, Kiyoko Higashiyama, Keisuke Yusa, Kazuhisa Uchida

  Jan. 2024, Engineering Reports


• Novel Approach for Obtaining Variable Domain of New Antigen Receptor with Different Physicochemical Properties from Japanese Topeshark (Hemitriakis japanica)

  Tomofumi Nakada-Masuta, Hiroyuki Takeda, Kazuhisa Uchida

  Oct. 2023, Marine Drugs


• Novel Approach for Obtaining VNAR with Different Physicochemical Properties from Japanese Topeshark (Hemitriakis japanica)

  Tomofumi Nakada-Masuta, Hiroyuki Takeda, Kazuhisa Uchida

  Oct. 2023


• Association between adeno‐associated virus genomic titers and intracellular plasmid levels

  Kyoko Masumi-Koizumi, yuzhe yuan, Kiyoko Higashiyama, Keisuke Yusa, KAZUHISA UCHIDA

  Wiley, Jun. 2023, Engineering Reports


• Quantitation of Residual Host Cell DNA in Recombinant Adeno-Associated Virus Using Droplet Digital Polymerase Chain Reaction

  Kiyoko Higashiyama, Yuzhe Yuan, Noriko Hashiba, Kyoko Masumi-Koizumi, Keisuke Yusa, Kazuhisa Uchida

  Jun. 2023, Human Gene Therapy


• Quantitation of Residual Host Cell DNA in Recombinant Adeno-Associated Virus Using Droplet Digital Polymerase Chain Reaction

  Kiyoko Higashiyama, Yuzhe Yuan, Noriko Hashiba, Kyoko Masumi-Koizumi, Keisuke Yusa, KAZUHISA UCHIDA

  Mary Ann Liebert Inc, Jun. 2023, Human Gene Therapy, 34(11-12) (11-12), 578 - 585


• Investigating critical thermal parameters for pre-analytical preparation of adeno-associated virus vector genome titration by droplet digital polymerase chain reaction

  Shunsuke SAITO, Akihiko KONDO, Kazuhisa UCHIDA

  {AMED} {iD}3 Catalyst Unit, 2023, Translational and Regulatory Sciences


• Practice of Human Resource Development Related to Biopharmaceutical Manufacturing and Quality Control and the International Contribution as Centers of Excellence of Asia-Pacific Economic Cooperation-Life Sciences Innovation Forum-Regulatory Harmonization Steering Committee

  Kazuhisa Uchida

  Pharmaceutical Society of Japan, Jul. 2022, YAKUGAKU ZASSHI, 142(7) (7), 749 - 753


• Infectivity assessment of porcine endogenous retrovirus using high-throughput sequencing technologies

  Kono, K., Kataoka, K., Yuan, Y., Yusa, K., Uchida, K., Sato, Y.

  2021, Biologicals, 71


• Viral safety testing for biopharmaceuticals: Current and future prospects

  Keisuke YUSA, Yuzhe YUSA, Kazuhisa UCHIDA

  {AMED} {iD}3 Catalyst Unit, 2020, Translational and Regulatory Sciences, 2(3) (3), 94 - 99


• A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

  Kono, K., Kataoka, K., Yuan, Y., Yusa, K., Uchida, K., Sato, Y.

  2020, Scientific Reports, 10(1) (1)


• A chromatographic approach for elevating the antibody-dependent cellular cytotoxicity of antibody composites

  Tojo, S., Okazaki, A., Wakitani, M., Shinkawa, T., Uchida, K., Suzawa, T.

  2009, Biological and Pharmaceutical Bulletin, 32(9) (9)


• Glycoform-dependent conformational alteration of the Fc region of human immunoglobulin G1 as revealed by NMR spectroscopy

  Yamaguchi, Y., Nishimura, M., Nagano, M., Yagi, H., Sasakawa, H., Uchida, K., Shitara, K., Kato, K.

  2006, Biochimica et Biophysica Acta - General Subjects, 1760(4) (4)


• Enhanced Fc-dependent cellular cytotoxicity of Fc fusion proteins derived from TNF receptor II and LFA-3 by fucose removal from asn-linked oligosaccharides

  Shoji-Hosaka, E., Kobayashi, Y., Wakitani, M., Uchida, K., Niwa, R., Nakamura, K., Shitara, K.

  2006, Journal of Biochemistry, 140(6) (6)


• Fucose removal from complex-type oligosaccharide enhances the antibody-dependent cellular cytotoxicity of single-gene-encoded bispecific antibody comprising of two single-chain antibodies linked to the antibody constant region

  Natsume, A., Wakitani, M., Yamane-Ohnuki, N., Shoji-Hosaka, E., Niwa, R., Uchida, K., Satoh, M., Shitara, K.

  2006, Journal of Biochemistry, 140(3) (3)


• Fucose removal from complex-type oligosaccharide enhances the antibody-dependent cellular cytotoxicity of single-gene-encoded antibody comprising a single-chain antibody linked the antibody constant region

  Natsume, A., Wakitani, M., Yamane-Ohnuki, N., Shoji-Hosaka, E., Niwa, R., Uchida, K., Satoh, M., Shitara, K.

  2005, Journal of Immunological Methods, 306(1-2) (1-2)


• IgG subclass-independent improvement of antibody-dependent cellular cytotoxicity by fucose removal from Asn²⁹⁷-linked oligosaccharides

  Niwa, R., Natsume, A., Uehara, A., Wakitani, M., Iida, S., Uchida, K., Satoh, M., Shitara, K.

  2005, Journal of Immunological Methods, 306(1-2) (1-2)


• Establishment of FUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity

  Yamane-Ohnuki, N., Kinoshita, S., Inoue-Urakubo, M., Kusunoki, M., Iida, S., Nakano, R., Wakitani, M., Niwa, R., Sakurada, M., Uchida, K., Shitara, K., Satoh, M.

  2004, Biotechnology and Bioengineering, 87(5) (5)


• Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA

  Mori, K., Kuni-Kamochi, R., Yamane-Ohnuki, N., Wakitani, M., Yamano, K., Imai, H., Kanda, Y., Niwa, R., Iida, S., Uchida, K., Shitara, K., Satoh, M.

  2004, Biotechnology and Bioengineering, 88(7) (7)


• Fucose Depletion from Human IgG1 Oligosaccharide Enhances Binding Enthalpy and Association Rate between IgG1 and FcγRIIIa

  Okazaki, A., Shoji-Hosaka, E., Nakamura, K., Wakitani, M., Uchida, K., Kakita, S., Tsumoto, K., Kumagai, I., Shitara, K.

  2004, Journal of Molecular Biology, 336(5) (5)


• The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity

  Shinkawa, T., Nakamura, K., Yamane, N., Shoji-Hosaka, E., Kanda, Y., Sakurada, M., Uchida, K., Anazawa, H., Satoh, M., Yamasaki, M., Hanai, N., Shitara, K.

  2003, Journal of Biological Chemistry, 278(5) (5)


• Capillary column high-performance liquid chromatographic-electrospray ionization triple-stage quadrupole mass spectrometric analysis of proteins separated by two-dimensional polyacrylamide gel electrophoresis application to cerebellar protein mapping

  Nakayama, H., Uchida, K., Shinkai, F., Shinoda, T., Okuyama, T., Seta, K., Isobe, T.

  1996, Journal of Chromatography A, 730(1-2) (1-2)


• Nuclear localization of p70-like protein in neurons of the superior colliculus and substantia nigra of the gerbil

  Seto-Ohshima, A., Karasawa, N., Nagatsu, I., Uchida, K., Isobe, T., Imai, S., Ozono, S., Onozuka, M.

  1995, Acta Histochemica et Cytochemica, 28(5) (5)


• Similarity of ribosomal proteins studied by two-dimensional coelectrophoresis for identification of gram-positive bacteria

  Ochiai, K., Uchida, K., Kawamoto, I.

  1993, International Journal of Systematic Bacteriology, 43(1) (1)


• Automated two-dimensional liquid chromatographic system for mapping proteins in highly complex mixtures

  Isobe, T., Uchida, K., Taoka, M., Shinkai, F., Manabe, T., Okuyama, T.

  1991, Journal of Chromatography A, 588(1-2) (1-2)


• Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver

  Sakurada, K., Uchida, K., Yamaguchi, K., Aisaka, K., Ito, S., Ohmori, T., Takeyama, Y., Ueda, T., Hori, Y., Ohyanagi, H., Saitoh, Y., Kaibuchi, K., Takai, Y.

  1991, Biochemical and Biophysical Research Communications, 177(3) (3)

• バイオ医薬品の製造・開発と規制上の課題

  UCHIDA KAZUHISA

  バイオ医薬品の製造・開発と規制上の課題の列挙と解決法の提示

  レギュラトリーサイエンス学会, May 2017, レギュラトリーサイエンス学会誌, 7(2) (2), 121 - 129

• バイオ医薬品の品質管理等に関わる人材育成プログラムの開発

  内田 和久

  日本医療研究開発機構, 創薬基盤推進研究事業, 2015