Research activity information

• 知財部門の機能拡大に関する研究 －知財関連業務への積極的関与－

  中村俊則(セコム;小委員長),富畑賢司(グンゼ:小委員長補佐),荒井良之(アンリツ),鵜篭芳直(サトーホールディングス),中村広希(デンソー),船田斉(昭和電工),増田一郎(沖電気工業),鎗居龍太(パナソニック)

  2016, 知財管理, 66, 64 - 78


• Regeneration of elastic fibers by three-dimensional culture on a collagen scaffold and the addition of latent TGF-β binding protein 4 to improve elastic matrix deposition.

  Rino Aya, Toshihiro Ishiko, Kazuo Noda, Satoko Yamawaki, Yuki Sakamoto, Kenji Tomihata, Yasuhiro Katayama, Katsuhiro Yoshikawa, Hiroshi Kubota, Tomoyuki Nakamura, Motoko Naitoh, Shigehiko Suzuki

  The objective of this study was to investigate the effects of latent TGF-β binding protein 4 (LTBP-4) on elastic fiber regeneration in three-dimensional cultures of human dermal fibroblasts (HDFs). Appropriate collagen scaffold for elastic fiber regeneration was also examined. Collagen sponges cross-linked at 120 °C and composed of small pores (25 μm on average) was favorable for elastic fiber regeneration by HDFs. Addition of LTBP-4, followed by culture for 21 days, accelerated elastic fiber accumulation within the scaffolds. Conditioned scaffolds containing either HDFs or LTBP-4-built mature elastic fibers were implanted between the dermis and the cutaneous muscle of mice. The combined use of HDFs and LTBP-4 resulted in thicker tissues containing elastic fibers. These results indicate that weakly cross-linked collagen sponges can be used as scaffolds for regenerating elastic fibers both in vitro and in vivo, and that the addition of LTBP-4 accelerates the deposition of both elastin and fibrillin-1, and increases cell proliferation. These techniques may be useful for generating cutaneous or cardiovascular tissue equivalents; furthermore, they may serve as a useful method for the three-dimensional analyses of drugs used to treat skin diseases or to examine the microstructure of elastin networks.

  Elsevier BV, Dec. 2015, Biomaterials, 72, 29 - 37, English, International magazine

  Scientific journal


• 知財戦略を実践するためのマネジメント―戦略的な出願活動の糸口が掴めずに悩んでいる企業への提言―

  山田貞美 (富士フイルム), 宮本哲也 (住友ベークライト), 荒井良之 (アンリツ), 太田一広 (三菱重工業), 岡潤 (清水建設), 熊本十美男 (ダンロップスポーツ), 佐藤裕介 (中外製薬), 鈴木雅彦 (ダイセル), 富畑賢司 (グンゼ)

  2014, 知財管理, 64, 1201 - 1215


• 知財業務におけるアウトソーシングマネジメントに関する研究

  中田知久 (帝人ファーマ), 小菅真理子 (NEC), 鵜篭芳直 (日産自動車), 遠藤隆 (リコー), 遠藤充彦 (富士ゼロックス), 葛見俊之 (住友重機械工業), 柴田木保子 (富士通), 富畑賢司 (グンゼ), 中山千里 (バッファロー), 水本大介 (日立), 宮田敦久 (NTTコムウェア)

  2013, 知財管理, 63, 1747 - 1761


• グローバル化,オープンイノベーション時代における社内知財教育に関する研究

  葛尾巧 (クレハ),濱田三和 (大建工業), 大野芳昭 (エヌ・テイ・テイ・データ), 高杉康史 (TDK), 津田智康 (クラレ), 富畑賢司 (グンゼ), 仲井幹夫 (ポリプラスチックス), 中山千里 (バッファロー), 宮崎亮智 (NTTドコモ)

  2012, 知財管理, 62, 977 - 992


• Preparation of collagen/gelatin sponge scaffold for sustained release of bFGF.

  Satoru Takemoto, Naoki Morimoto, Yu Kimura, Tsuguyoshi Taira, Tatsuya Kitagawa, Kenji Tomihata, Yasuhiko Tabata, Shigehiko Suzuki

  Artificial dermis (AD) has been used to regenerate dermis-like tissues in the treatment of full-thickness skin defects, but it takes 2 or 3 weeks to complete dermal regeneration. Our previous study demonstrated that injection of basic fibroblast growth factor (bFGF)-impregnated gelatin microspheres (MS) into the AD accelerates the regeneration of dermis-like tissue. However, injection of gelatin MS before clinical use is complicated and time consuming. This study investigated a new scaffold, in which collagen and gelatin are integrated, and which is capable of sustained bFGF release. We produced collagen/gelatin sponges with a gelatin concentration of 0wt%, 10wt%, 30wt%, and 50wt%. The mean pore size in each sponge decreased with the gelatin concentration. In an in vitro study, proliferation of fibroblasts in each sponge was not significantly different over 7 days of culture. As for in vivo sustained release of bFGF, a radioisotope study demonstrated that retention of bFGF in gelatin 10wt% and 30wt% sponges was significantly larger than that in gelatin 0wt% sponge. The collagen/gelatin sponges were grafted on full-thickness skin defects created on a rabbit ear, and we evaluated regeneration of dermis-like tissue by measuring the amount of hemoglobin and size of dermis-like tissue on histological sections. Seven days after implantation, the amount of hemoglobin in dermis-like tissue in gelatin 10wt% sponge was significantly larger than those in control and gelatin 50wt% sponge. Twenty-eight days after implantation, the area of dermis-like tissue in gelatin 10wt% sponge was significantly larger than those in the other specimens. We conclude that the collagen sponge integrated with 10wt% gelatin has the most potential for sustained release of bFGF and that the combination of collagen/gelatin 10wt% sponge and bFGF is a promising therapeutic modality for the treatment of full-thickness skin defects.

  Mary Ann Liebert Inc, Oct. 2008, Tissue engineering. Part A, 14(10) (10), 1629 - 38, English, International magazine

  Scientific journal


• In vivo culturing of a bilayered dermal substitute with adipo-stromal cells.

  Naoki Morimoto, Satoru Takemoto, Takeshi Kawazoe, Shigehiko Suzuki, Kenji Tomihata, Tsuguyoshi Taira

  BACKGROUND: Skin grafting is an important procedure to cover skin defects. Recently, cultured epidermal sheets and bilayered cultured skin have been used clinically, but they lack subcutaneous tissue. The objective of this study was to produce a bilayered dermal substitute with adipose tissue simultaneously in vivo. MATERIALS AND METHODS: We disseminated adipo-stromal cells on one side of a collagen sponge at a density of 1,0 x 10(5)cells/cm(2) and incubated overnight. Then, we turned over the sponge and disseminated dermal fibroblasts and keratinocytes at a density of 1,0 x 10(6)cells/cm(2) on the other side of the sponge. Finally, we cultured this for 1 wk and implanted it on the backs of severe combined immunodeficiency mice with or without basic FGF. RESULTS: Six weeks after implantation, specimens were harvested. Macroscopically, the formed tissue in the bFGF-administered group was thick, and the epidermal component, the dermal component, and adipose tissue were formed in the cross section. The thickness of newly formed tissue in bFGF-administered group was significantly greater than that in the group without bFGF administration. The area of the newly formed capillaries in the bFGF-administered group was significantly larger than that in the group without bFGF administration. CONCLUSIONS: We could produce a thick composite tissue in vivo, combining three kinds of human cells, collagen scaffold, and bFGF. This composite graft was thicker than the bilayered dermal substitute and could be a substitute for a skin flap.

  May 2008, The Journal of surgical research, 146(2) (2), 246 - 53, English, International magazine

  Scientific journal


• Alveolar bone regeneration using absorbable poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblast growth factor.

  Y Kinoshita, M Matsuo, K Todoki, S Ozono, S Fukuoka, H Tsuzuki, M Nakamura, K Tomihata, T Shimamoto, Y Ikada

  The aim of this study was to evaluate the effects of combining a porous poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblastic growth factor (bFGF) on bone regeneration in mandibular ridges. Four full-thickness saddle-type defects (10 mm long x 5 mm deep) were symmetrically created in both edentulous mandibular alveolar ridges of 6 beagles. The dome-shaped membrane was secured to each defect site, and a gelatin sponge containing 200 microg bFGF was implanted on the left side of each defect (experimental group). Only the membranes (control group) were secured to the defect sites on the right. Three and 6 months later, 3 animals were killed. Bone regeneration was analyzed by soft X-ray photographs, micro-computed tomography (CT) images, and peripheral quantitative CT (pQCT), and then examined histologically. Soft X-ray examination revealed an increase in new bone volume in the experimental group 6 months postoperatively. pQCT showed that immature bone density was higher in the experimental group. Micro-CT images revealed well formed new bone along the original contour of the dome-shaped membrane in the experimental group. Histologically, inflammatory infiltration of tissue surrounding the membranes was slight. These results suggest that combining the poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and bFGF-gelatin sponge is promising for alveolar ridge reconstruction.

  Mar. 2008, International journal of oral and maxillofacial surgery, 37(3) (3), 275 - 81, English, International magazine

  Scientific journal


• Collagen/gelatin scaffold for sustained release of bFGF

  Satoru Takemoto, Naoki Morimoto, Norikazu Kanda, Kimura Yu, Tsuguyoshi Taira, Tatsuya Kitagawa, Kenji Tomihata, Yasuhiko Tabata, Shigehiko Suzuki

  2008, 8th World Biomaterials Congress 2008, 4, 1790, English

  International conference proceedings


• Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation.

  Yasumi Tsuji-Saso, Takeshi Kawazoe, Naoki Morimoto, Yasuhiko Tabata, Tsuguyoshi Taira, Kenji Tomihata, Atsushi Utani, Shigehiko Suzuki

  Cultured skin substitutes (CSS) with both epidermal and dermal components seem to be ideal, but they have not been widely used clinically, partly because it takes several weeks to produce them. Decreasing the number of seeding cells may reduce the period required for production, but it still takes a long time before the cells become confluent and neovascularisation is completed in CSS after grafting. As we have already succeeded in reducing the number of seeded keratinocytes in this study, we first attempted to reduce the number of seeded fibroblasts. Consequently, preconfluent CSS with 10 x 10(3) cells/cm2 of fibroblasts combined with 100 x 10(3) cells/cm2 of keratinocytes could be successfully grafted on to full-thickness wounds. bFGF-impregnated gelatin microspheres were then added to the preconfluent CSS before grafting. Incorporation of bFGF significantly accelerated neovascularisation and increased epidermal thickness, cellular components, and thickness of the dermis. The incorporation of bFGF makes CSS a potential therapeutic approach for management of skin wounds.

  Informa UK Limited, Jan. 2007, Scandinavian journal of plastic and reconstructive surgery and hand surgery, 41(5) (5), 228 - 35, English, International magazine

  Scientific journal


• 再生医療研究に利用可能な三次元培養皮膚モデル

  森川 訓行, 諸田 勝保, 富畑 賢司, 平 嗣良, 高橋 佳丈, 森田 真一郎, 鈴木 昌和

  日本再生歯科医学会, Dec. 2005, 日本再生歯科医学会誌, 3(1) (1), 12 - 22, Japanese


• 【眼科における最新医工学】生体材料工学 生体吸収性縫合糸

  富畑 賢司, 鈴木 昌和

  (株)医学書院, Oct. 2005, 臨床眼科, 59(11) (11), 322 - 327, Japanese


• Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation.

  Naoki Morimoto, Yasumi Saso, Kenji Tomihata, Tsuguyoshi Taira, Yoshitake Takahashi, Masayoshi Ohta, Shigehiko Suzuki

  BACKGROUND: Fibroblast-seeded collagen sponges have been used for the treatment of skin defects and skin ulcers. However, the viability of the fibroblasts after implantation is still unknown. The objective of this study was to investigate the viability and distribution of autologous and allogeneic fibroblasts after implantation and to clarify which type is more effective for wound healing. MATERIALS AND METHODS: Skin samples of Hartley guinea pigs were retrieved and autologous fibroblasts were isolated and cultured. Fibroblasts isolated from the skin of a Strain2 guinea pig were used as allogeneic fibroblasts. Three full-thickness wounds were created on the backs of guinea pigs and an acellular collagen sponge, a collagen sponge seeded with autologous fibroblasts, and a collagen sponge seeded with allogeneic fibroblasts were transplanted. Before implantation, fibroblasts were labeled with PKH26. The guinea pigs were sacrificed 1, 2, and 3 weeks after implantation. The epithelization and contraction of the wounds were assessed, and the viability and distribution of the seeded fibroblasts were observed in cross sections. RESULTS: Three weeks after implantation, the PKH26-labeled autologous and allogeneic fibroblasts remained viable. In the wounds covered with the autologous fibroblast-seeded collagen sponge, the epithelization was fastest, and the percent wound contraction was smallest. In contrast, in the wounds covered with allogeneic fibroblasts, the epithelization was slowest and the percent contraction was largest. CONCLUSION: The allogeneic fibroblasts seeded in the collagen sponge survived and remained viable on the grafted area, but did not accelerate wound healing.

  May 2005, The Journal of surgical research, 125(1) (1), 56 - 67, English, International magazine

  Scientific journal


• Handling characteristics of poly(L-lactide-co-epsilon-caprolactone) monofilament suture.

  Kenji Tomihata, Masakazu Suzuki, Naohide Tomita

  A monofilament suture made of poly(L-lactide-co-epsilon-caprolactone) was examined by several mechanical tests to evaluate handling characteristics for tight tying. Six types of other monofilament sutures were also examined for comparisons. Two of these were nonabsorbable, while the others were absorbable sutures. Sutures consisting of glicolide were strongest among all the sutures examined. On the other hand, PROLENE and P(LA/CL) sutures showed high knot-pull strength despite low straight pull strength. Untying performance was evaluated by viscoelasticity, bending plasticity and tying test. A good correlation between tan delta and bending plasticity index was observed and the poly(L-lactide-co-epsilon-caprolactone) suture exhibited high tan delta, high bending plasticity and good resistance against untying.

  2005, Bio-medical materials and engineering, 15(5) (5), 381 - 91, English, International magazine

  Scientific journal


• 培養人工皮膚作製時の培地の培養皮膚生着に及ぼす影響

  佐生 泰美, 川添 剛, 森本 尚樹, 鈴木 茂彦, 富畑 賢司

  培養人工皮膚作製時におけるウシ胎仔血清(FCS)の添加が培養皮膚接着に及ぼす影響について検討した.その結果,in vitroでの培養人工皮膚における細胞増殖においては播種した線維芽細胞,表皮角化細胞とも,FCS添加により細胞増殖は有意に促進された.しかし,FCS添加の有無にかかわらず,免疫抑制動物に移植した培養人工皮膚は生着し,表皮は重層化され,基底層・有棘層・顆粒層・角層の形成を認めた

  (一社)日本熱傷学会, Sep. 2004, 熱傷, 30(3) (3), 143 - 151, Japanese


• 培養人工皮膚作成に使用する培地の検討

  佐生 泰美, 鈴木 茂彦, 川添 剛, 富畑 賢司, 森田 真一郎

  (一社)日本熱傷学会, Nov. 2003, 熱傷, 29(4) (4), 212 - 213, Japanese


• Cell-preconfluent培養皮膚への徐放性bFGF添加効果

  佐生 泰美, 川添 剛, 郷司 みちよ, 鈴木 茂彦, 田畑 泰彦, 富畑 賢司, 森田 真一郎

  bFGF含浸ゼラチンマイクロスフィアーを培養皮膚に組み込み,培養皮膚の上皮化促進効果を検討した.移植4日目のbFGF徐放群では対照群と比較してコラーゲンスポンジの下層に線維芽細胞の侵入が明らかで,又,多くの赤血球も確認でき,血管の新生が始まっていると考えられた.移植7日目には,bFGF徐放群は真皮層のかなり上層まで多くの線維芽細胞,赤血球が確認できた.移植2週間後ではbFGF徐放群,対象群とも表皮層は重層化し,コラーゲンスポンジ内も上層まで線維芽細胞侵入がみられるが,bFGF徐放群の方が表皮の重層化は厚く,角層も観察できた.bFGF徐放群では表皮層下縁に沿って染色され,基底膜の再構築が進んた.ゼラチンマイクロスフィアーを用いbFGFを徐放させることで,cell-preconfluentな培養皮膚の移植後上皮化完了促進効果が認められた

  (一社)日本熱傷学会, Mar. 2003, 熱傷, 29(1) (1), 14 - 20, Japanese


• 熱傷モデルとしての培養皮膚による創傷被覆材の皮膚刺激性試験

  伊藤 理, 鈴木 茂彦, 宗内 巌, 川添 剛, 佐藤 美樹, 佐生 泰美, 小野寺 正征, 郷司 みちよ, 森川 訓行, 富畑 賢司, 森田 真一郎

  (一社)日本熱傷学会, Nov. 2002, 熱傷, 28(4) (4), 273 - 274, Japanese


• 最近の生体吸収性縫合糸の動向

  富畑 賢司

  日本バイオマテリアル学会, Aug. 2001, 生体材料, 19(4) (4), 143 - 147, Japanese


• Sensitizer contained in heat-decomposed dye.

  K Tomihata, M Suzuki, H Sato, M Kitagawa

  Recently a new resorbable P(LA/CL) monofilament suture made of L-lactide and epsilon-caprolactone was developed. The sensitization test was carried out using a guinea pig maximization test according to the Japanese Guidelines. As a result of this test, it was revealed that the methanol extract of P(LA/CL) suture contains a skin sensitizer. It was suggested that the D&C Violet No. 2 contained in P(LA/CL) sutures is decomposed during the melt-spinning process and that the decomposed dye induces skin sensitization. To characterize the sensitizer (the decomposed dye) it was separated and collected by HPLC. To determine the molecular formula of the sensitizer, MS, IR, and NMR spectra were obtained. The molecular weight of the sensitizer was estimated as 240 by measuring the MS. Considering the chemical structure of the dye and the molecular weights, it was determined that the sensitizer was 1,4-dihydroxyanthraquinone (quinizarin). The D&C Violet No. 2 contained in P(LA/CL) sutures was decomposed by the melt-spinning procedure to form quinizarin, one type of anthraquinone dye, and the sensitizer was identified to be, indeed, quinizarin. To evaluate the sensitizing potential of quinizarin, we tested skin sensitization using the maximization test method. Quinizarin induced skin sensitization in guinea pigs, and the minimum sensitizing concentration was approximately 100 ppm. The concentration of quinizarin contained in the P(LA/CL) suture was 1-5 ppm, a value that is lower than the minimum sensitizing concentration of quinizarin. This suggests that P(LA/CL) suture has a low risk with regard to contact dermatitis.

  Wiley, Mar. 2001, Journal of biomedical materials research, 54(4) (4), 531 - 9, English, International magazine

  Scientific journal


• The pH dependence of monofilament sutures on hydrolytic degradation.

  K Tomihata, M Suzuki, Y Ikada

  Hydrolytic degradation of two nonabsorbable sutures, four absorbable sutures, and a new type of absorbable suture was studied in buffered media of various pHs at 37 degrees C. The pH levels fixed in this study were 1.0, 7.4, 8.5, and 10.5. Physical measurements were made on the retention of tensile strength and melting temperature of the sutures after hydrolysis for 12 weeks. Sutures containing glycolic acid as a comonomer exhibited enhanced degradation in alkaline media, similar to polyglycolide multifilament sutures. Poly-p-dioxanone (PDS II) suture lost strength to a significant extent at pH 1.0, suggesting that care should be taken when this suture is used for closing tissues in contact with acidic media, such as the stomach. In marked contrast, the degradation of lactide-epsilon-caprolactone copolymer [P(LA/CL)] suture was not sensitive to the pH of media. The surface morphology of hydrolyzed sutures varied, depending on the pH of media. Particularly, moon-crater-shaped impressions were observed on glycolide-epsilon-caprolactone copolymer (MONOCRYL) and glycolide-trimethylene carbonate-dioxanone copolymer (BIOSYN) sutures. Among the nonabsorbable sutures, nylon (ETHILON) exhibited the fastest loss of strength in acidic buffer solution, and polypropylene (PROLENE) suture retained most of its initial strength at all pHs studied.

  Wiley, 2001, Journal of biomedical materials research, 58(5) (5), 511 - 8, English, International magazine

  Scientific journal


• Cross-linking of amniotic membranes.

  T Fujisato, K Tomihata, Y Tabata, Y Iwamoto, K Burczak, Y Ikada

  Human amniotic membrane was cross-linked with chemical and radiation methods to investigate the effect of cross-linking on its physicochemical and biodegradation properties. Radiation cross-linking was performed with gamma-ray and electron beam while chemical cross-linking was with glutaraldehyde (GA). Both gamma-ray and electron beam irradiation decreased the tensile strength and elongation at break of the amniotic membrane with an increase in the irradiation dose, whereas GA cross-linking had no effect on the tensile properties. This is probably due to the scission of collagen chains through irradiation. No significant change was observed on the water content of cross-linked amniotic membranes for any of the crosslinking methods and in marked contrast with cross-linking of a gelatin membrane. A permeation study revealed that protein permeation through the amniotic membrane was not influenced by the GA concentration at cross-linking. These findings are ascribed to the structure characteristic of the amniotic membrane. The membrane is composed of a fibrous mesh structure from an assemblage of collagen fibers. It is possible that cross-linking takes place in the interior of the fiber assembly without impairing the mesh structure, resulting in no change of the water content and protein permeability. In vitro degradation of cross-linked amniotic membranes revealed that radiation cross-linking appeared to be much less effective than GA cross-linking in retarding the degradation, probably because of low cross-linking densities. GA-cross-linked amniotic membranes were degraded more slowly as the GA concentration at cross-linking increased. When the GA-cross-linked amniotic membrane was subcutaneously implanted in the rat, the tissue response was mild, similar to that of the non-cross-linked native membrane.

  Informa UK Limited, Jan. 1999, Journal of biomaterials science. Polymer edition, 10(11) (11), 1171 - 81, English, International magazine

  Scientific journal


• A new resorbable monofilament suture

  K. Tomihata, M. Suzuki, T. Oka, Y. Ikada

  Elsevier BV, Jan. 1998, Polymer Degradation and Stability, 59(1-3) (1-3), 13 - 18

  Scientific journal


• Crosslinking of hyaluronic acid with glutaraldehyde

  Kenji Tomihata, Yoshito Ikada

  Wiley, Nov. 1997, Journal of Polymer Science Part A: Polymer Chemistry, 35(16) (16), 3553 - 3559

  Scientific journal


• Crosslinking of hyaluronic acid with water-soluble carbodiimide.

  K Tomihata, Y Ikada

  Hyaluronic acid (HA) was chemically crosslinked with a water-soluble carbodiimide (WSC) to produce low-water-content films when brought into contact with water. The crosslinking reaction was performed in two different ways; one was by using HA films and the other by casting HA solutions. Both methods produced water-insoluble HA films. The lowest water content of the crosslinked HA films subjected to swelling with water was 60 wt % at 37 degrees C, which was lower than any reported values. Infrared spectra of the crosslinked films suggested that intermolecular formation of ester bonds between the hydroxyl and carboxyl groups belonging to different polysaccharide molecules led to crosslinking. For comparison, pectin which possesses hydroxyl and carboxyl groups in one molecule, similar to HA, was subjected to crosslinking with WSC. The finding on pectin also supported ester formation between different polysaccharide molecules. The crosslinking of HA film with WSC in the presence of L-lysine methyl ester prolonged the in vivo degradation of HA film, probably because of amide bond formation as the crosslink.

  Nov. 1997, Journal of biomedical materials research, 37(2) (2), 243 - 51, English, International magazine

  Scientific journal


• In vitro and in vivo degradation of films of chitin and its deacetylated derivatives.

  K Tomihata, Y Ikada

  Chitin was deacetylated to various extents with NaOH to obtain partially and thoroughly deacetylated chitins. The specimens used in this study were deacetylated by 0 (chitin), 68.8, 73.3, 84.0, 90.1 and 100 mol% (chitosan). Films with a thickness of 150 microns were prepared from these specimens by the solution casting method. The equilibrated water contents of the films were 52.4 (chitin), 73.8 (68.8 mol%), 64.2 (73.3 mol%), 61.8 (84.0 mol%), 57.8 (90.1 mol%) and 49.7 wt% (chitosan), while the tensile strengths of the water-swollen films were 244 (chitin), 197 (68.8 mol%), 232 (73.3 mol%), 320 (84.0 mol%), 293 (90.1 mol%) and 433 g mm-2 (chitosan). The maximum water content and the minimum tensile strength observed for a specimen deacetylated between 0 and 68.8 mol% may be ascribed to the lowered crystallinity by deacetylation of chitin, since both chitin and chitosan are crystalline polymers. Unlike their physical properties, in vitro and in vivo degradations of these films occurred less rapidly without passing a maximum or minimum, as their degree of deacetylation became higher. The in vitro degradation was carried out by immersing the films in buffered aqueous solution of pH 7 containing lysozyme at 37 degrees C, while the in vivo degradation was studied by subcutaneously implanting the films in the back of rats. It was found that the rate of in vivo biodegradation was very high for chitin and 68.8 mol% deacetylated chitin, compared with that for the 73.3 mol% deacetylated chitin. The films which were more than 73.3 mol% deacetylated showed slower biodegradation. Interestingly, the tissue reaction towards highly deacetylated derivatives including chitosan was very mild, although they had cationic primary amines in the molecule.

  Elsevier BV, Apr. 1997, Biomaterials, 18(7) (7), 567 - 75, English, International magazine

  Scientific journal


• Preparation of cross-linked hyaluronic acid films of low water content.

  K Tomihata, Y Ikada

  Hyaluronic acid (HA) was chemically cross-linked with poly(ethylene glycol) diglycidyl ether, a diepoxy compound (EX-810), to yield low water content and slowly degradable films when brought into contact with water. The cross-linking reaction was performed under acidic and neutral conditions, since the epoxy group is readily hydrolysed in alkaline media. To allow the reaction to proceed at high HA concentrations, a solution casting method was employed for the cross-linking of HA. The lowest water content of the cross-linked HA films obtained was 60 wt% when swollen with buffered saline at 37 degrees C. Alginic acid and poly(vinyl alcohol), which possess hydroxyl groups, similar to HA, were also found to undergo cross-linking with the diepoxy compound. Since IR spectra of the cross-linked films had no significantly new absorption, intermolecular formation of ether bonds between the hydroxyl groups belonging to different polysaccharide molecules was assumed to take place. It seemed too difficult to detect the ether bonds in the cross-linked HA films, because the virgin HA film itself contained ether bonds in the molecule. The cross-linked HA film with a water content of 60 wt% exhibited practically no weight loss after 10 days of immersion in phosphate-buffered saline (pH 7.4), while this film underwent in vivo degradation by 30% weight loss after 7 days of subcutaneous implantation in rats. The inflammation reaction elicited around the implanted film was not significant.

  Elsevier BV, Feb. 1997, Biomaterials, 18(3) (3), 189 - 95, English, International magazine

  Scientific journal


• Cross-linking of gelatin with carbodiimides.

  K Tomihata, Y Ikada

  Carbodiimides were employed for cross-linking of gelatin through amide bond formation to avoid any foreign bond incorporation into the cross-linked gelatin molecules. Cross-linking of gelatin was performed not in aqueous solution but in the form of a film under a heterogenous condition. Ethanol-water mixtures were used as the reaction medium to prevent dissolution of gelatin films. The optimal ethanol concentration in the ethanol-water mixtures was around 80 vol%, and the water content of the cross-linked gelatin film attained after swelling with water at 25 degrees C was as low as 55 wt%. A water-soluble carbodiimide (l-ethyl-3[3-dimethylaminoprophyl] carbodiimide) was more effective for gelatin cross-linking than a water-insoluble carbodiimide (1,3-dicyclohexyl carbodiimide). The optimal temperature for cross-linking with the water-soluble carbodiimide ranged between 15 and 25 degrees C. It was concluded that gelatin cross-linking with carbodiimides is as effective as that with glutaraldehyde, which is most widely used despite the toxicity, at least, so far as the water content of cross-linked gelatin film is concerned.

  1996, Tissue engineering, 2(4) (4), 307 - 13, English, International magazine

  Scientific journal


• Experimental study on a new tracheal prosthesis made from collagen-conjugated mesh.

  N Okumura, T Nakamura, T Natsume, K Tomihata, Y Ikada, Y Shimizu

  A new tracheal prosthesis was made from fine Marlex mesh reinforced with a continuous polypropylene spiral. The mesh and spiral were covalently grafted and further coated with pig collagen with the aim of promoting connective tissue infiltration and providing initial airtightness. Complete surgical resection and replacement of a segment (2 cm in length, three to five tracheal rings) of the cervical trachea was performed in 13 adult mongrel dogs. Two dogs died of pneumonia about 2 months after operation, and eleven dogs were killed between 3 and 26 months. The prostheses in all dogs were promptly infiltrated by the surrounding connective tissue and completely incorporated by the host trachea. Formation of respiratory epithelium, which lined the prosthetic lumen, was seen to various degrees, and, in five dogs killed at 6 months or more after reconstruction, confluent epithelialization was confirmed histologically from the upper to the lower anastomotic site of the prosthesis. Marked stenosis of the prosthetic lumen caused by excessive scar tissue growth was seen in three dogs, and ulceration on the luminal surface was seen in two dogs. These results indicate that this tracheal prosthesis is highly biocompatible and promising for the repair of tracheal defects after further investigation.

  Aug. 1994, The Journal of thoracic and cardiovascular surgery, 108(2) (2), 337 - 45, English, International magazine

  Scientific journal


• EXPERIMENTAL STUDY OF A NEW TRACHEAL PROSTHESIS MADE FROM COLLAGEN-GRAFTED MESH; INVESTIGATION OF APPLICATION TO INTRATHORACIC TRACHEAL REPLACEMENT

  OKUMURA N., TERAMACHI M., NAKAMURA T., TAKIMOTO Y., KIYOTANI T., NATSUME T., SHIMIZU Y., TOMIHATA K., IKADA Y.

  We carried out intrathoracic tracheal replacement in dogs using our original tracheal prosthesis made from mesh. The prosthesis consists of Marlex® mesh (polypropylene) reinforced with a continuous polypro-pylene spiral, and is grafted and coated with pig collagen. Complete surgical resection of the mediastinal trachea was performed in 6 adult mongrel dogs. In one dog, a 4-tracheal-ring segment (2cm) was resected and replaced with a 3-cm-long prosthesis, and in 5 dogs, a 7-to 8-tracheal-ring segment (4cm) was resected and replaced with a 5-cm-long prosthesis. In the latter 5 dogs, a silicone tube was temporarily inserted into the replacement, and removed by bronchoscopy one month after surgery. The prostheses in all dogs were promptly infiltrated by surrounding tissue and completely incorporated by the host trachea. No dehiscence or air leakage was observed after surgery. Mild luminal stenosis was evident in one dog, and partial exposure of the mesh (ulceration) was observed in 5 dogs within an observation period of 4 to 26 months. Formation of respiratory epithelium, which lined the prosthetic lumen, was seen to various degrees, and in the dog which received the 2-cm-long replacement, epithelization was confirmed histologically from the upper to the lower anastomosis of the prosthesis. We conclude that our tracheal prosthesis is useful for the repair of intrathoracic tracheal defects and shows promise for clinical application.

  JAPANESE SOCIETY FOR ARTIFICIAL ORGANS, 1994, Journal of the Japanese Society for Artificial Organs and Tissues, 23(3) (3), 878 - 882


• Experimental study of a tracheal prosthesis made from collagen-grafted mesh: Investigation of anastomosis under high tension

  N. Okumura, T. Nakamura, T. Natsume, M. Teramachi, T. Kiyotani, Y. Takimoto, X.G. Lee, K. Tomihata, Y. Ikada, S. Hitomi, Y. Shimizu

  We attempted to perform tracheal replacement using a mesh-type prosthesis under high tension. The prosthesis, 3 cm in length, consists of Marlex mesh (polypropylene) reinforced with a continuous polypropylene spiral, and is grafted and coated with pig collagen. Complete surgical resection of an 11- to 16-tracheal-ring segment of the cervical trachea with a tension of 900 to 1000gf on both stumps was performed in 10 adult mongrel dogs. In 4 of the dogs (group A), simple replacement with the prosthesis was done, whereas in the other 6 dogs (group B), a silicone tithe was temporarily inserted into the replacement, and then removed by bronchoscopy 1 month after surgery. No anastomotic insufficiency was seen in any of the dogs. In group A, local infection and luminal stenosis due to overgrowth of granulation were often observed. In group B, however, no such findings were evident, and in a dog which was eiuthanatized 3 months after surgery, the tracheal prosthesis was completely incorporated by the host, together with formation of respiratory epithelium, which lined the prosthesis lumen. We conclude that our tracheal prosthesis is highly biocompatible, and that it can be used for tracheal replacement under tension, suggesting availability of clinical application. Temporary insertion of a silicone tube also proved to be useful for prevention of local infection at the site of the material.

  JAPANESE SOCIETY FOR ARTIFICIAL ORGANS, 1993, Japanese Journal of Artificial Organs, 22(3) (3), 1025 - 1030, English

  [Refereed]


• A new tracheal prosthesis made from collagen grafted mesh.

  N Okumura, T Nakamura, Y Takimoto, T Natsume, M Teramachi, K Tomihata, Y Ikada, Y Shimizu

  The authors studied the efficacy of a new tracheal prosthesis made from mesh. The prosthesis, 50 mm long and 18-22 mm in diameter, is made from a Marlex mesh cylinder reinforced with a continuous polypropylene spiral that is grafted and coated with porcine collagen to increase its biocompatibility and provide an airtight seal during the initial implantation stage. Circumferential surgical resection and replacement of a seven to nine ring segment of the cervical trachea was performed in 20 adult mongrel dogs. At the time of surgery, a silicone tube was inserted into the tracheal prosthetic lumen to promote secretory transportation until the prosthesis was covered with host tissue. The silicone tube was removed during fiber bronchoscopy 1 month after surgery. With the exception of the prostheses in 3 dogs that died of unrelated causes, all were infiltrated by connective tissue and incorporated completely by the host. One of these 17 dogs died of suffocation caused by luminal stenosis 2.5 months after surgery, but the others survived until they were killed at more than 6 months. The luminal surfaces of the reconstructed tracheae were covered with respiratory epithelium to varying degrees, and in one dog killed at 22 months after surgery, confluent epithelization throughout the length of the prosthesis was confirmed histologically. In eight dogs, prosthetic luminal stenosis occurred because of overgrowth of granulation tissue, which generally was mild in all but three dogs. The authors conclude that this tracheal prosthesis is highly biocompatible and shows promise for clinical application.

  1993, ASAIO journal (American Society for Artificial Internal Organs : 1992), 39(3) (3), M475-9 - 479, English, International magazine

  Scientific journal


• An experimental study on the effects of gelatin membrane for preventing adhesion after tendon repair (2nd report)

  H.Ishida, S.Tamai, N.Tomita, K.Tomihata, Y.Ikada

  1993, J. Jpn. Soc. Surg. Hand,, 10, 215 - 219


• Experimental study of a nerve guide-tube mode from dehydrothemally treated gelatin application to repair of gap in rat sciatic nerve.

  LEE G., NAKAMURA T., SHIMIZU Y., TOMIHATA K., IKADA Y., ENDO K.

  In order to evaluate the regeneration of resected nerves, a dehydrothermally treated gelatin tube was used to repair 10-mm defects in the sciantic nerve of rats. As a control, a gelatin tube treated with glutaraldehyde was used. Histological examination using Osmium, Bodian and anti-S-100 protein staining at 2 months and at 4 months after the operations revealed that the regenerated axons reached through the gelatin guide-tube to the distal end.
  Motoneurons of the ventral horn as well as afferent nerve terminals in the dorsal horn of the spine were stained both retrogradely and anterogradely with WGA-HRP, indicating nerve regeneration through the bridged area. With regard to nerve function, the electromyogram and cerebral somatosensory potentials at 2 months showed functional recovery.
  In the controls, no regeneration was observed. These results indicate that this dehydrothermally treated gelatin tube is a promising biomaterial for use as a nerve-conductive tube for inducing nerve regeneration.

  JAPANESE SOCIETY FOR ARTIFICIAL ORGANS, 1993, Journal of the Japanese Society for Artificial Organs and Tissues, 22(2) (2), 364 - 369, Japanese


• The repair of tracheal defects using bioabsorbable mesh.

  N Okumura, T Nakamura, Y Takimoto, T Kiyotani, Y H Lee, Y Shimizu, K Tomihata, Y Ikada, K Shiraki

  This study compares the use of synthetic bioabsorbable mesh with that of non-absorbable mesh in repairing tracheal defects. Two patch windows, each 1 cm x 1 cm, were created on the ventral wall of the cervical trachea in 12 adult mongrel dogs. One window was repaired using a bioabsorbable polyglycolic acid (PGA) mesh graft, and the other using a nonabsorbable Marlex mesh (polypropylene mesh; PP mesh) (C. R. Bard, Inc., Billerica, MA) graft. The dogs were killed at intervals from 1 to 35 weeks after surgery. Macroscopically, there was no difference between the two mesh groups until 3 weeks after surgery. At 6 weeks in the PP mesh group, the connective tissue over the mesh was remarkably thick, and after 9 weeks, constriction by scarlike tissue was evident, as compared with the PGA mesh group, which showed no constriction. Microscopically, epithelium covered the whole mesh in both groups at 6 weeks. However, in the PP mesh group, flat and poorly differentiated cells occupied a large area, and after 9 weeks the height of the epithelial cells in the PP mesh group was less than in the PGA mesh group. Furthermore, in the PP mesh group, nonciliated cells occupied a large area even at 15 weeks, and the submucous connective tissue layer was thicker than in the PGA mesh group after 6 weeks. The results indicate that, from the point of view of wound-healing, bioabsorbable mesh may be more adequate for repairing tracheal defects.

  1992, ASAIO journal (American Society for Artificial Internal Organs : 1992), 38(3) (3), M555-9 - 559, English, International magazine

  Scientific journal

• 知的財産情報を活用した医療現場ニーズとシーズの効率的なマッチングの試み(第2報)

  富畑賢司, 中西淑人, 小嶋章夫, 穴井博文

  2019, 産学連携学会大会講演予稿集(CD-ROM), 17th


• 医看工芸連携活動に寄与する知財マインドを高めるためのしくみづくり

  富畑賢司, 野田佳邦, 加藤幹, 吉田悦子, 富田直秀, 辰巳明久

  2019, 日本知財学会年次学術研究発表会(CD-ROM), 17th


• 特許情報を活用した医療現場ニーズとシーズの効率的なマッチングの試み

  富畑賢司, 中西淑人, 山本喜大, 穴井博文

  2018, 産学連携学会大会講演予稿集(CD-ROM), 16th


• 医看工芸連携活動における対象者別の知的財産教育プログラムの開発

  富畑賢司, 野田佳邦, 北村英隆, 吉田悦子, 富田直秀, 辰巳明久

  2018, 日本知財学会年次学術研究発表会(CD-ROM), 16th


• 医看工芸連携活動に関わる人への知的財産教育プログラムの開発

  富畑賢司, 野田佳邦, 北村英隆, 吉田悦子, 富田直秀, 辰巳明久

  2017, 日本知財学会年次学術研究発表会(CD-ROM), 15th


• 医療現場ニーズから生まれる機器開発における知的財産保護と,関係者への知的財産教育プログラム作成の取り組み

  富畑賢司, 野田佳邦, 北村英隆, 吉田悦子, 富田直秀, 辰巳明久

  2016, 日本知財学会年次学術研究発表会(CD-ROM), 14th


• In vitroにおける三次元的弾性線維の再生

  石河利広, 内藤素子, 坂元悠紀, 富畑賢司, 松田昌二郎, 吉川勝宇, 山脇聖子, 鈴木茂彦

  2010, 日本形成外科学会基礎学術集会プログラム・抄録集, 19th


• 自己血清を用いた自家培養真皮作製方法の検討

  森本尚樹, 武本啓, 神田則和, 鈴木茂彦, 富畑賢司, 平嗣良, 北川達哉

  2008, 再生医療, 7


• 自家培養真皮の正式認可を目指しての問題点の解決及び臨床試験プロトコル作成

  森本尚樹, 武本啓, 神田則和, 鈴木茂彦, 富畑賢司, 平嗣良, 坂元悠紀

  2008, 日本形成外科学会基礎学術集会プログラム・抄録集, 17th


• コラーゲン/ゼラチンスポンジに添加する塩基性線維芽細胞増殖因子至適濃度の検討

  神田 則和, 武本 啓, 森本 尚樹, 鈴木 茂彦, 北川 達也, 富畑 賢司, 平 嗣良

  (一社)日本創傷治癒学会, Dec. 2007, 日本創傷治癒学会プログラム・抄録集, 37回, 73 - 73, Japanese


• bFGF徐放可能なコラーゲン/ゼラチンスポンジ作製

  武本 啓, 森本 尚樹, 木村 祐, 平 嗣良, 北川 達哉, 富畑 賢司, 田畑 泰彦, 鈴木 茂彦

  (一社)日本炎症・再生医学会, Jul. 2007, Inflammation and Regeneration, 27(4) (4), 404 - 404, Japanese


• 自家培養真皮臨床応用への取り組み

  森本 尚樹, 武本 啓, 富畑 賢司, 平 嗣良, 鈴木 茂彦

  (一社)日本組織移植学会, Jul. 2007, 日本組織移植学会雑誌, 6(1) (1), 66 - 66, Japanese


• B-mode and C-mode Imaging of Regenerated 3D Skin Model with 100MHz Ultrasound

  SAIJO Yoshifumi, HAGIWARA Yoshihiro, KOBAYASHI Kazuto, OKADA Nagaya, TANAKA Akira, HOZUMI Naohiro, TOMIHATA Kenji

  2007, Proceedings. IEEE Ultrasonics Symposium, 244 - 247

  Summary international conference


• 三次元培養系における間葉系幹細胞の増殖能ならびに分化能の非破壊的評価方法の開発-第2報

  森川訓行, 富畑賢司, 北川達哉

  2007, 再生医療, 6


• 幹細胞等を用いた細胞組織医療機器の開発と評価技術の標準化 総合研究報告

  土屋利江, 松岡厚子, 澤田留美, 中岡竜介, 片倉健男, 増田茂樹, 田村知明, 平谷治之, 坂井正宗, 富畑賢司, 小川哲朗, 阿蘇雄, 富田直秀, 脇谷滋之, 吉川秀樹, 新岡俊治, 澤芳樹, 中村直仁, 刈谷豊

  2007, 政策創薬総合研究重点研究報告書2 平成18年度


• 幹細胞等を用いた細胞組織医療機器の開発と評価技術の標準化 18年度報告

  土屋利江, 松岡厚子, 澤田留美, 中岡竜介, 片倉健男, 増田茂樹, 田村知明, 平谷治之, 坂井正宗, 富畑賢司, 小川哲朗, 阿蘇雄, 富田直秀, 脇谷滋之, 吉川秀樹, 新岡俊治, 澤芳樹

  2007, 政策創薬総合研究重点研究報告書2 平成18年度


• 自己血清を用いた線維芽細胞培養法の検討

  森本尚樹, 武本啓, 神田則和, 鈴木茂彦, 富畑賢司, 平嗣良, 北川達哉

  2007, 日本形成外科学会基礎学術集会プログラム・抄録集, 16th


• コラーゲンスポンジを用いた自家培養真皮臨床応用への取り組み

  森本 尚樹, 武本 啓, 川添 剛, 富畑 賢司, 平 嗣良, 鈴木 茂彦

  日本バイオマテリアル学会, Nov. 2006, 日本バイオマテリアル学会大会予稿集, 28回, 105 - 105, Japanese


• 吸収性GBR膜;βTCP含有Poly(L-lactide-co-ε-caprolactone)シートとbFGF含浸ゼラチンスポンジとの併用による歯槽骨再生

  木下 靱彦, 松尾 雅斗, 塗々木 和男, 小園 知, 福岡 真一, 富畑 賢司, 島本 偉志, 筏 義人

  日本バイオマテリアル学会, Nov. 2006, 日本バイオマテリアル学会大会予稿集, 28回, 245 - 245, Japanese


• 創薬等ヒューマンサイエンス研究重点研究報告書 医療材料および製剤設計技術の開発に関する研究 幹細胞等を用いた細胞組織医療機器の開発と評価技術の標準化

  土屋利江, 松岡厚子, 澤田留美, 中岡竜介, 片倉健男, 増田茂樹, 森山剛, 平谷治之, 坂井正宗, 富畑賢司, 小川哲朗, 阿蘇雄, 富田直秀, 脇谷滋之, 吉川秀樹, 新岡俊治, 澤芳樹

  2006, 創薬等ヒューマンサイエンス研究重点研究報告書, 479 - 499


• ウシ血清を用いない培養真皮作製方法の検討

  森本尚樹, 武本啓, 川添剛, 鈴木茂彦, 富畑賢司, 平嗣良

  2006, 日本形成外科学会基礎学術集会プログラム・抄録集, 15th


• コラーゲン/ゼラチンスポンジによるbFGF徐放

  武本啓, 森本尚樹, 鈴木茂彦, 平嗣良, 富畑賢司, 木村祐, 田畑泰彦

  2006, 日本形成外科学会基礎学術集会プログラム・抄録集, 15th


• 培養皮弁作成の試み

  森本 尚樹, 武本 啓, 川添 剛, 富畑 賢司, 平 嗣良

  (一社)日本創傷治癒学会, Dec. 2005, 日本創傷治癒学会プログラム・抄録集, 35回, 54 - 54, Japanese


• コラーゲンスポンジを足場とした培養皮膚,培養真皮の作製と今後の展望

  森本尚樹, 武本啓, 川添剛, 鈴木茂彦, 辻泰美, 富畑賢司, 平嗣良

  2005, 日本形成外科学会基礎学術集会プログラム・抄録集, 14th


• 自家,同種線維芽細胞の創傷治癒に与える影響

  森本 尚樹, 鈴木 茂彦, 佐生 泰美, 富畑 賢司, 平 嗣良, 高橋 佳丈, 森川 訓行

  (一社)日本熱傷学会, Nov. 2004, 熱傷, 30(4) (4), 212 - 212, Japanese


• 培養皮膚の血管新生

  森本尚樹, 鈴木茂彦, 辻泰美, 川添剛, 武本啓, 田畑泰彦, 富畑賢司, 平嗣良

  2004, 日本形成外科学会基礎学術集会プログラム・抄録集, 13th


• 同種,自家培養真皮における線維芽細胞の移植後の動態

  森本 尚樹, 鈴木 茂彦, 佐生 泰美, 富畑 賢司, 平 嗣良, 高橋 佳丈, 森川 訓行

  (一社)日本創傷治癒学会, Dec. 2003, 日本創傷治癒学会プログラム・抄録集, 33回, 42 - 42, Japanese


• 培養人工皮膚作成に使用する培地の再検討

  佐生泰美, 鈴木茂彦, 川添剛, 富畑賢司

  2003, 日本熱傷学会総会・学術集会プログラム・抄録集, 29th


• 培養人工皮膚作成時に使用する培地の検討

  佐生泰美, 川添剛, 森本尚樹, 池田実香, 鈴木茂彦, 森川訓行, 平嗣良, 富畑賢司

  2003, 日本形成外科学会基礎学術集会プログラム・抄録集, 12th


• 培養皮膚による褥瘡モデルについて(第1報)

  伊藤 理, 鈴木 茂彦, 宗内 巖, 川添 剛, 佐藤 美樹, 佐生 泰美, 小野寺 年征, 森川 訓行, 富畑 賢司, 森田 真一郎

  (一社)日本褥瘡学会, Apr. 2002, 日本褥瘡学会誌, 4(1) (1), 118 - 118, Japanese


• バイオマテリアルの生体反応 医療用具製造工程中での原材料の変性により皮膚感作性が観察された一例

  富畑 賢司, 鈴木 昌和, 佐藤 秀隆, 北川 雅士

  日本バイオマテリアル学会, Nov. 1999, 日本バイオマテリアル学会大会予稿集, 21回, 147 - 147, Japanese


• Basic research on mechanical and biological characteristics of amnion. (Ministry of Education S)

  岩本喜恵, 藤里俊哉, 富畑賢司, 田畑泰彦, 筏義人

  1996, 生体機能と構造の維持・回復・強化のバイオメカニクス 平成4-6年度


• In vitro degradation of biopolymer.

  岩本喜恵, 富畑賢司, 筏義人

  1993, 高分子学会予稿集, 42(3) (3)


• An experimental study on the effects of gelatin membrane for preventing adhesions after tendon repair - first report

  H.Ishida, N.Tomita, S.Tamai, K.Tomihata, Y.Ikada

  1993, Cent. Jpn. J. Orthop. Traumat., 36, 373 - 374

• 最近の知的財産における諸課題 藤本昇先生喜寿記念論文集

  藤本昇先生喜寿記念論文集編集委員会

  Contributor, 現場から見た産学連携における知財をめぐる課題, 一般社団法人発明推進協会, Sep. 2024, ISBN: 9784827114065


• 生分解性高分子

  富畑賢司, 筏義人

  Joint work, 架橋生体高分子ゲル（p.19-28）, 高分子刊行会, 1994, ISBN: 4770200706

• 医看工芸連携活動に寄与する知財マインドを高めるためのしくみづくり

  富畑賢司, 野田佳邦, 加藤幹, 吉田悦子, 富田直秀, 辰巳明久

  日本知財学会年次学術研究発表会, Dec. 2019

  Oral presentation


• 知的財産について

  富畑 賢司

  医療機器開発ビジネススクール, Sep. 2019

  Public discourse


• 事業活動に必要な 知的財産の基礎知識 ～特許だけではない知的財産～

  富畑 賢司

  第４３期マネジメントスクール, Jul. 2019

  Public discourse


• 知的財産情報を活用した医療現場ニーズとシーズの効率的なマッチングの試み(第2報)

  富畑賢司, 中西淑人, 小嶋章夫, 穴井博文

  産学連携学会大会, Jun. 2019

  Oral presentation


• 大分大学および大分県内高等教育機関 における知的財産教育の実施状況 ～共同利用拠点との連携～

  富畑 賢司

  知財教育シンポジウム in 田町2019, Mar. 2019

  Nominated symposium


• 医療現場ニーズを基にした 医療機器開発と知財戦略 ～特許だけではない知的財産～

  富畑 賢司

  医工連携における知財、デザイン の考え方セミナー, Mar. 2019

  Public discourse


• 医看工芸連携活動における対象者別の知的財産教育プログラムの開発

  富畑賢司, 野田佳邦, 北村英隆, 吉田悦子, 富田直秀, 辰巳明久

  日本知財学会年次学術研究発表会, Dec. 2018

  Oral presentation


• 医療機器開発の知財戦略 ～特許だけではない知的財産～

  富畑 賢司

  医療機器分野参入セミナー （応用コース）, Nov. 2018

  Public discourse


• 医療現場ニーズを基にした医療機器開発と知的財産 ～特許情報の活用による効率的な開発～

  富畑 賢司

  九州地方自治体の真ブランドづくり支援事業（大分ミーティング）, Oct. 2018

  Public discourse


• 特許情報を活用した医療現場ニーズとシーズの効率的なマッチングの試み

  富畑賢司, 中西淑人, 山本喜大, 穴井博文

  産学連携学会大会, Jun. 2018

  Oral presentation


• 医看工芸連携活動に関わる人への知的財産教育プログラムの開発

  富畑賢司, 野田佳邦, 北村英隆, 吉田悦子, 富田直秀, 辰巳明久

  日本知財学会年次学術研究発表会, Dec. 2017

  Oral presentation


• 医工連携活動における 知的財産管理の基礎知識 ～医療現場ニーズと知的財産～

  富畑 賢司

  第11回単孔式内視鏡手術研究会, Aug. 2017

  Invited oral presentation


• ゆるキャラからオリンピックまで ～身近にある知的財産～

  富畑 賢司

  平成２８年度 大分市産学交流サロン, Jan. 2017

  Public discourse


• 医療現場ニーズから生まれる機器開発における知的財産保護と,関係者への知的財産教育プログラム作成の取り組み

  富畑賢司, 野田佳邦, 北村英隆, 吉田悦子, 富田直秀, 辰巳明久

  日本知財学会年次学術研究発表会, Dec. 2016

  Oral presentation


• 知的財産管理と保護

  富畑 賢司

  医療機器開発ビジネススクール, Aug. 2016

  Public discourse


• 知財活用と知財マネジメント ～自分の魅力を認識して発信するために～

  富畑 賢司

  平成２７年度 宇佐市産学交流会, Dec. 2015

  Public discourse


• 知的財産管理と保護

  富畑 賢司

  医療機器開発研修会, Aug. 2015

  Public discourse


• In vitroにおける三次元的弾性線維の再生

  石河利広, 内藤素子, 坂元悠紀, 富畑賢司, 松田昌二郎, 吉川勝宇, 山脇聖子, 鈴木茂彦

  日本形成外科学会基礎学術集会, 2010

  Oral presentation


• 自家培養真皮の正式認可を目指しての問題点の解決及び臨床試験プロトコル作成

  森本尚樹, 武本啓, 神田則和, 鈴木茂彦, 富畑賢司, 平嗣良, 坂元悠紀

  日本形成外科学会基礎学術集会, 2008

  Oral presentation


• 自家培養真皮臨床応用への取り組み

  森本尚樹, 武本啓, 富畑賢司, 平嗣良, 鈴木茂彦

  日本組織移植学会, Jul. 2007

  Oral presentation


• コラーゲン/ゼラチンスポンジに添加する塩基性線維芽細胞増殖因子至適濃度の検討

  神田則和, 武本啓, 森本尚樹, 鈴木茂彦, 北川達也, 富畑賢司, 平嗣良

  日本創傷治癒学会, 2007

  Oral presentation


• bFGF徐放可能なコラーゲン/ゼラチンスポンジ

  武本啓, 森本尚樹, 木村祐, 平嗣良, 北川達哉, 富畑賢司, 田畑泰彦, 鈴木茂彦

  日本形成外科学会基礎学術集会, 2007

  Oral presentation


• 自己血清を用いた線維芽細胞培養法の検討

  森本尚樹, 武本啓, 神田則和, 鈴木茂彦, 富畑賢司, 平嗣良, 北川達哉

  日本形成外科学会基礎学術集会, 2007

  Oral presentation


• 吸収性GBR膜;βTCP含有Poly(L-lactide-co-ε-caprolactone)シートとb FGF含浸ゼラチンスポンジとの併用による歯槽骨再生

  木下靭彦, 松尾雅斗, 塗々木和男, 小園知, 福岡真一, 富畑賢司, 島本偉志, 筏義人

  日本バイオマテリアル学会, Nov. 2006

  Oral presentation


• 生体吸収性医療用具の開発

  富畑賢司

  近畿バイオインダストリー振興会議第１２回組織工学・再生医学ワークショップ, Apr. 2006

  [Invited]

  Nominated symposium


• コラーゲン/ゼラチンスポンジによるbFGF徐放

  武本啓, 森本尚樹, 鈴木茂彦, 平嗣良, 富畑賢司, 木村祐, 田畑泰彦

  日本形成外科学会基礎学術集会, 2006

  Oral presentation


• コラーゲンスポンジを用いた自家培養真皮臨床応用への取り組み

  森本尚樹, 武本啓, 川添剛, 富畑賢司, 平嗣良, 鈴木茂彦

  日本バイオマテリアル学会, 2006

  Oral presentation


• ウシ血清を用いない培養真皮作製方法の検討

  森本尚樹, 武本啓, 川添剛, 鈴木茂彦, 富畑賢司, 平嗣良

  日本形成外科学会基礎学術集会, 2006

  Oral presentation


• コラーゲンスポンジを足場とした培養皮膚,培養真皮の作製と今後の展望

  森本尚樹, 武本啓, 川添剛, 鈴木茂彦, 辻泰美, 富畑賢司, 平嗣良

  日本形成外科学会基礎学術集会, 2005

  Oral presentation


• 自家,同種線維芽細胞の創傷治癒に与える影響

  森本尚樹, 鈴木茂彦, 佐生泰美, 富畑賢司, 平嗣良, 高橋佳丈, 森川訓行

  日本熱傷学会総会, 2004

  Oral presentation


• 培養皮膚の血管新生

  森本尚樹, 鈴木茂彦, 辻泰美, 川添剛, 武本啓, 田畑泰彦, 富畑賢司, 平嗣良

  日本形成外科学会基礎学術集会, 2004

  Oral presentation


• 培養人工皮膚作成時に使用する培地の検討

  佐生泰美, 川添剛, 森本尚樹, 池田実香, 鈴木茂彦, 森川訓行, 平嗣良, 富畑賢司

  日本形成外科学会基礎学術集会, Oct. 2003

  Oral presentation


• 自家培養真皮における線維芽細胞の移植後の動態

  森本尚樹, 鈴木茂彦, 佐生泰美, 富畑賢司, 高橋佳丈, 森川訓行, 平嗣良

  日本形成外科学会基礎学術集会, Oct. 2003

  Oral presentation


• 培養人工皮膚作成に使用する培地の再検討

  佐生泰美, 鈴木茂彦, 川添剛, 富畑賢司

  日本熱傷学会総会, Jun. 2003

  Oral presentation


• 医療用具製造工程中での原材料の変性により皮膚感作性が観察された一例

  富畑賢司, 鈴木昌和, 佐藤秀隆, 北川雅士

  日本バイオマテリアル学会, Nov. 1999

  Oral presentation


• 新しい吸収性モノフィラメント縫合糸の研究

  富畑賢司, 鈴木昌和, 岡高茂, 筏義人

  繊維学会シンポジウム, Jun. 1997

  Oral presentation


• ヒアルロン酸の架橋反応と分解

  富畑賢司, 筏義人

  繊維学会シンポジウム, Jun. 1994

  Oral presentation


• ヒアルロン酸の架橋について

  富畑賢司, 筏義人

  高分子学会, May 1994

  Oral presentation


• 架橋ヒアルロン酸の生体内分解

  富畑賢司, 筏義人

  高分子学会, May 1993

  Oral presentation


• 生体高分子のin vitro分解

  岩本喜恵, 富畑賢司, 筏義人

  高分子学会, May 1993


• Apatite-gelatin composites

  Kenji Tomihata, Kaneto Shiraki, Yoshito Ikada

  アパタイト研究会, Dec. 1992

  Oral presentation


• ヒト羊膜の分解制御

  岩本喜恵, 富畑賢司, Krystyna Burczak, 筏義人

  日本バイオマテリアル学会, Nov. 1992

  Oral presentation


• ゼラチンの分解吸収の制御

  富畑賢司, 白木兼人, 筏義人

  日本バイオマテリアル学会シンポジウム, Nov. 1992

  Oral presentation


• コラーゲン加工メッシュ製人工気管の実験的研究

  奥村典仁, 中村達雄, 夏目徹, 滝本行延, 清水慶彦, 富畑賢司, 筏義人

  日本胸部外科学会総会, Oct. 1992

  Oral presentation


• 架橋ゼラチンの生体内分解

  富畑賢司, 白木兼人, 筏義人

  医用高分子シンポジウム, Jun. 1992

  Oral presentation


• ヒアルロン酸の架橋反応

  富畑賢司, BURCZAK K., 筏義人

  高分子学会, May 1992


• コラーゲン加工メッシュ製人工気管の実験的研究ー高張力下での吻合に関してー

  奥村典仁, 中村達雄, 夏目徹, 寺町政美, 清谷哲也, 滝本行延, 清水慶彦, 筏義人, 人見滋樹

  日本人工臓器学会, 1992

  Oral presentation


• 人工神経の研究ーゼラチンチューブによる末梢神経再生チャンネルの開発ー

  李暁光, 中村達雄, 清水慶彦, 富畑賢司, 筏義人, 遠藤克昭

  日本人工臓器学会, 1992

  Oral presentation

• 日本バイオマテリアル学会

  Apr. 2025 - Present


• 日本生体医工学会

  Apr. 2025 - Present


• 日本人工臓器学会

  Apr. 2025 - Present


• 日本再生医療学会

  Apr. 2015 - Present


• 日本知財学会

  Apr. 2015 - Present


• 日本弁理士会

  Jan. 2008 - Present

• 科学技術イノベーション時代の医療機器開発教育における知的財産教育プログラム開発

  富畑 賢司

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, Apr. 2025 - Mar. 2028, Principal investigator


• 国際競争力のある国産医療機器創成に資する知財戦略に関する調査研究

  公益財団法人 高橋産業経済研究財団, 助成金, 神戸大学未来医工学研究開発センター, Apr. 2025 - Mar. 2027, Principal investigator


• 特許情報を活用した医看工芸連携活動の促進と、大学教職員等への知的財産情報検索研修プログラム開発

  一般財団法人工業所有権協力センター, 大学知財活動助成事業, 大分大学, Apr. 2018 - Mar. 2020, Principal investigator


• 大学等が保有する知財ビジネスマッチング事業～産学官連携によるオープンイノベーション～

  九州経済産業局, 中小企業知的財産活動支援事業補助金（地域中小企業知的財産支援力強化事業）, 大分大学, Apr. 2018 - Mar. 2019


• デザイン要素を組み込んだ医療・福祉機器開発連携活動の活性化と新しい医工連携活動の仕組み構築事業

  九州経済産業局, 中小企業知的財産活動支援事業補助金（地域中小企業知的財産支援力強化事業）, 大分大学, Apr. 2017 - Mar. 2018


• 医看工芸連携活動に寄与する知的財産教育プログラム開発

  一般財団法人工業所有権協力センター, 大学知財活動助成事, 大分大学, Apr. 2016 - Mar. 2018, Principal investigator

• 培養皮膚の製造方法、及び、弾性線維組織層を有する培養皮膚

  鈴木 茂彦, 内藤 素子, 中邨 智之, 富畑 賢司

  特願2008-211084, 19 Aug. 2008, グンゼ株式会社, 鈴木 茂彦, 特開2010-045984, 04 Mar. 2010, 特許第5360869号, 13 Sep. 2013

  Patent right

  IRI


• 弾性線維組織を有する培養血管の製造方法及び弾性線維組織を有する培養血管

  鈴木 茂彦, 内藤 素子, 中邨 智之, 富畑 賢司

  特願2007-291969, 09 Nov. 2007, グンゼ株式会社, 鈴木 茂彦, 特開2009-112285, 28 May 2009, 特許第5252538号, 26 Apr. 2013

  Patent right

  IRI


• 三次元培養弾性線維組織及び三次元培養弾性線維組織の製造方法

  鈴木 茂彦, 内藤 素子, 中邨 智之, 富畑 賢司

  特願2007-291136, 08 Nov. 2007, グンゼ株式会社, 国立大学法人京都大学, 特開2009-112277, 28 May 2009, 特許第5224440号, 22 Mar. 2013

  Patent right

  IRI


• 組織再生用基材

  筏 義人, 鈴木 茂彦, 平 嗣良, 高橋 佳丈, 富畑 賢司

  特願2005-261840, 09 Sep. 2005, グンゼ株式会社, 特開2007-068884, 22 Mar. 2007, 特許第5008284号, 08 Jun. 2012

  Patent right

  IRI


• 培養皮膚の製造方法、及び、弾性線維組織層を有する培養皮膚

  鈴木 茂彦, 内藤 素子, 中邨 智之, 富畑 賢司

  特願2008-211084, 19 Aug. 2008, 鈴木 茂彦, 特開2010-045984, 04 Mar. 2010

  Patent right

  IRI


• 結節強力を向上させた医療用縫合糸

  大越 豊, 富畑 賢司

  特願平11-125420, 06 May 1999, グンゼ株式会社, 特開2000-312714, 14 Nov. 2000, 特許第4421004号, 11 Dec. 2009

  Patent right

  IRI


• 収縮性の改善された人工皮膚

  鈴木 茂彦, 富畑 賢司, 平 嗣良, 稲継 泰之

  特願2002-289573, 02 Oct. 2002, グンゼ株式会社, 特開2004-121523, 22 Apr. 2004, 特許第4373658号, 11 Sep. 2009

  Patent right

  IRI


• 弾性線維組織を有する培養血管の製造方法及び弾性線維組織を有する培養血管

  鈴木 茂彦, 内藤 素子, 中邨 智之, 富畑 賢司

  特願2007-291969, 09 Nov. 2007, グンゼ株式会社, 鈴木 茂彦, 特開2009-112285, 28 May 2009

  Patent right

  IRI


• 三次元培養弾性線維組織及び三次元培養弾性線維組織の製造方法

  鈴木 茂彦, 内藤 素子, 中邨 智之, 富畑 賢司

  特願2007-291136, 08 Nov. 2007, グンゼ株式会社, 国立大学法人京都大学, 特開2009-112277, 28 May 2009

  Patent right

  IRI


• 培養液交換器

  下地 広之, 富畑 賢司, 森川 訓行

  特願2003-056505, 04 Mar. 2003, グンゼ株式会社, 特開2004-261133, 24 Sep. 2004, 特許第4293518号, 17 Apr. 2009

  Patent right

  IRI


• 細胞組織医療用具の輸送容器

  稲継 泰之, 森川 訓行, 富畑 賢司

  特願2003-009569, 17 Jan. 2003, グンゼ株式会社, 特開2004-217290, 05 Aug. 2004, 特許第4190898号, 26 Sep. 2008

  Patent right

  IRI


• 間葉系幹細胞の分化を計測する方法

  森川 訓行, 富畑 賢司

  特願2006-054268, 01 Mar. 2006, グンゼ株式会社, 特開2007-228873, 13 Sep. 2007

  Patent right

  IRI


• 架橋多糖類及びその製造法並びにこれによる複合材料

  富畑 賢司, 岡 高茂, 筏 義人 (2/2)

  特願平7-166900, 07 Jun. 1995, グンゼ株式会社, 特開平8-333402, 17 Dec. 1996, 特許第3999823号, 17 Aug. 2007

  Patent right

  IRI


• 組織再生用基材

  筏 義人, 鈴木 茂彦, 平 嗣良, 高橋 佳丈, 富畑 賢司

  特願2005-261840, 09 Sep. 2005, グンゼ株式会社, 特開2007-068884, 22 Mar. 2007

  Patent right

  IRI


• 細胞培養基材およびこれの処理法

  岩田 博夫, 松田 晶二郎, 富畑 賢司

  特願2004-097577, 30 Mar. 2004, グンゼ株式会社, 特開2005-278504, 13 Oct. 2005

  Patent right

  IRI


• ゼラチンスポンジ

  高橋 佳丈, 平 嗣良, 富畑 賢司

  特願2004-024263, 30 Jan. 2004, グンゼ株式会社, 特開2005-213449, 11 Aug. 2005

  Patent right

  IRI


• 培養液交換器

  下地 広之, 富畑 賢司, 森川 訓行

  特願2003-056505, 04 Mar. 2003, グンゼ株式会社, 特開2004-261133, 24 Sep. 2004

  Patent right

  IRI


• 細胞組織医療用具の輸送容器

  稲継 泰之, 森川 訓行, 富畑 賢司

  特願2003-009569, 17 Jan. 2003, グンゼ株式会社, 特開2004-217290, 05 Aug. 2004

  Patent right

  IRI


• 手術用縫合糸及びその製造法

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