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ISHII Takashige
Graduate School of Agricultural Science / Department of Bioresource Science
Professor

Researcher basic information

■ Research Keyword
  • Plant Genetics
  • Plant Breeding
■ Research Areas
  • Environmental science/Agricultural science / Plant genetics and breeding
  • Life sciences / Biodiversity and systematics
  • Life sciences / Genetics
■ Committee History
  • 日本育種学会, 幹事

Research activity information

■ Award
  • Sep. 2018 日本育種学会, 第134回講演会日本育種学会優秀発表賞 (2), Exomeと果実・葉形状にみる日本のウメの多様性
    沼口孝司, 赤木剛士, 北村祐人, 大江孝明, ISHIKAWA RYO, ISHII TAKASHIGE
    Japan society

  • Sep. 2018 日本育種学会, 第134回講演会日本育種学会優秀発表賞, ジャポニカ型栽培イネ（日本晴）の非脱粒性に関与する新規遺伝子座の離層形成への効果
    杉山 昇平, 辻村 雄紀, 大塚 宏太郎, Htun, Than Myint, ISHII TAKASHIGE, ISHIKAWA RYO
    Japan society

  • Sep. 2016 日本育種学会, 第130回講演会日本育種学会優秀発表賞, Oryza rufipogonとインディカ型栽培イネ（カサラス）の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索
    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, ISHII TAKASHIGE, ISHIKAWA RYO
    Japan society

  • Sep. 2014 日本育種学会, 第126回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用と離層形態
    井上 千鶴, Than Myint Htun, INOUE KANAKO, IKEDA KENICHI, ISHII TAKASHIGE, ISHIKAWA RYO
    Japan society

  • Mar. 2014 日本育種学会, 第125回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用
    井上 千鶴, Than Myint Htun, ISHII TAKASHIGE, ISHIKAWA RYO
    Japan society

  • Oct. 2013 神戸大学, 学長表彰, 顕著な研究功績
    ISHII TAKASHIGE
    Others

  • Sep. 2013 六篠会, 六篠国際賞, 顕著な研究功績
    ISHII TAKASHIGE
    Others

■ Paper
  • Sathya Lim, Chhourn Orn, Hiroki Saito, Ryo Ishikawa, Yutaka Sato, Takashige Ishii
    Corresponding, Jun. 2024, Journal of Crop Research, 69, 1 - 7
    [Refereed]
    Scientific journal

  • Yoshiki Tokuyama, Miku Omachi, Shiori Kushida, Kiwamu Hikichi, Shuhei Okada, Kazumitsu Onishi, Takashige Ishii, Yuji Kishima, Yohei Koide
    Springer Science and Business Media LLC, Dec. 2023, Planta, 259(1) (1), English
    [Refereed]
    Scientific journal

  • Koji Numaguchi, Tomoaki Kashiwamoto, Ryo Ishikawa, Takashige Ishii, Yuto Kitamura
    Japanese apricot (Prunus mume) is an important fruit tree in East Asia. ‘Nanko’, the primary cultivar of Japanese apricots, usually suffers from scab, a disease caused by Venturia carpophila. However, there have been few reports on the phenotypic variation in scab resistance/susceptibility and the underlying genetic factors. In this study, we investigated the severity of naturally occurring scabs based on fruit lesions in 108 Japanese apricot accessions over four consecutive years. In each year, both resistant and susceptible accessions were observed, and significant annual correlations were detected among the ratios of diseased fruits (Rt; 0.52–0.76) and among the disease severity indices (Sv; 0.55–0.79). We also conducted a genome-wide association study (GWAS) based on exon-targeted resequencing, and significant peaks were detected in the data from 2017 and 2018. The candidate genes involved in disease resistance are located near nine single-nucleotide polymorphisms. These genes may be associated with the susceptibility of ‘Nanko’ lineages to scab. These findings shed light on the phenotypic and genetic profiles of scab resistance in P. mume and will assist future breeding programs with improving scab resistance.
    MDPI AG, Jul. 2023, Horticulturae, 9(8) (8), 872 - 872, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Shohei Sugiyama, Motoki Sakuta, Yuki Tsujimura, Yudai Yamaguchi, Than Myint Htun, Chizuru Inoue, Koji Numaguchi, Takashige Ishii, Ryo Ishikawa
    Asian rice (Oryza sativa) was domesticated from O. rufipogon, and reduced seed-shattering behaviour was selected to increase yields. Two seed-shattering loci, qSH3 and sh4, are involved in reducing seed shattering in both japonica and indica rice cultivars, while qSH1 and qCSS3 are likely specific to japonica cultivars. In indica cultivars, qSH3 and sh4 fail to explain the degree of seed shattering, as an introgression line (IL) of O. rufipogon W630 carrying domesticated alleles at qSH3 and sh4 still showed seed shattering. Here we analysed differences in seed-shattering degree between the IL and the indica cultivar IR36. The values for grain detachment in the segregating population between the IL and IR36 were continuous. Based on QTL-seq analysis using the BC1F2 population between the IL and IR36, we detected two novel loci, qCSS2 and qCSS7 (QTLs for the Control of Seed Shattering in rice on chromosomes 2 and 7), which contributed to the reduced seed shattering in IR36. We further investigated the genetic interaction of qCSS2 and qCSS7 under the presence of qSH3 and sh4 mutations in O. rufipogon W630 and found that IL carrying IR36 chromosomal segments covering all four loci are required to explain seed-shattering degree in IR36. Since qCSS2 and qCSS7 were not detected in previous studies on seed shattering in japonica, their control may be specific to indica cultivars. Therefore, they are important to understanding the history of rice domestication as well as to adjusting the seed-shattering degree of indica cultivars to maximise their yield.
    May 2023, Molecular genetics and genomics : MGG, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Phuong Dang Thai Phan, Akinori Nishimura, Chika Yamamoto, Pham Thien Thanh, Toshihiro Niwa, Yaddehige Priya Jayantha Amarasinghe, Ryo Ishikawa, Takashige Ishii
    Corresponding, Japanese Society of Breeding, 2023, Breeding Science, 73(4) (4), 373 - 381, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Sathya Lim, Anna Onoda, Chhourn Orn, Hiromu Iwamoto, Ryo Ishikawa, Hiroki Saito, Yutaka Sato, Takashige Ishii
    More than half-century ago, local rice varieties were collected from Indochinese countries (Cambodia, Thailand, Laos, and Vietnam). Of these, 162 local varieties were examined for 7 grain-size traits: seed length/width/thickness, brown rice length/width/thickness, and 100-seed weight. Since these traits varied considerably, a survey of functional mutations was performed in the genes related to these traits. In total, 19 markers (12 InDel and 7 dCAPS markers) were used to investigate the mutations at 14 grain-size loci of GW2, GS2, qLGY3, GS3, GL3.1, TGW3, GS5, GW5, GS6, TGW6, GW6a, GLW7, GL7, and GW8. Significant allele effects were observed with six markers detecting base substitution mutations at GW2 and GS3 and insertion/deletion mutations at GS5, GW5, and GW6a, suggesting that these mutations might have affected the grain trait and caused variation among local varieties in the Indochinese countries. In addition to grain size, the hull color, grain color, and glutinosity were also examined using a survey of loss-of-function mutations at major responsible loci. Most phenotypes were reflected based on functional mutations at these loci. Since the local varieties have wide genetic variation, they are important genetic resources for future rice breeding.
    Corresponding, Dec. 2022, Plants (Basel, Switzerland), 12(1) (1), English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryo Ishikawa, Cristina Cobo Castillo, Than Myint Htun, Koji Numaguchi, Kazuya Inoue, Yumi Oka, Miki Ogasawara, Shohei Sugiyama, Natsumi Takama, Chhourn Orn, Chizuru Inoue, Ken-Ichi Nonomura, Robin Allaby, Dorian Q Fuller, Takashige Ishii
    Asian rice (Oryza sativa L.) is consumed by more than half of the world's population. Despite its global importance, the process of early rice domestication remains unclear. During domestication, wild rice (Oryza rufipogon Griff.) acquired non-seed-shattering behavior, allowing humans to increase grain yield. Previous studies argued that a reduction in seed shattering triggered by the sh4 mutation led to increased yield during rice domestication, but our experiments using wild introgression lines show that the domesticated sh4 allele alone is insufficient for shattering loss in O. rufipogon. The interruption of abscission layer formation requires both sh4 and qSH3 mutations, demonstrating that the selection of shattering loss in wild rice was not as simple as previously suggested. Here we identified a causal single-nucleotide polymorphism at qSH3 within the seed-shattering gene OsSh1, which is conserved in indica and japonica subspecies but absent in the circum-aus group of rice. Through harvest experiments, we further demonstrated that seed shattering alone did not significantly impact yield; rather, yield increases were observed with closed panicle formation controlled by SPR3 and further augmented by nonshattering, conferred by integration of sh4 and qSH3 alleles. Complementary manipulation of panicle shape and seed shattering results in a mechanically stable panicle structure. We propose a stepwise route for the earliest phase of rice domestication, wherein selection of visible SPR3-controlled closed panicle morphology was instrumental in the sequential recruitment of sh4 and qSH3, which together led to the loss of shattering.
    Jun. 2022, Proceedings of the National Academy of Sciences of the United States of America, 119(26) (26), e2121692119, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Miki Ogasawara, Naoya Miyazaki, Gotaro Monden, Kenta Taniko, Sathya Lim, Masahide Iwata, Takashige Ishii, Jian Feng Ma, Ryo Ishikawa
    Sep. 2021, THEORETICAL AND APPLIED GENETICS, 134(9) (9), 3013 - 3022, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yaddehige P.J. Amarasinghe, Mitsuharu Otsuka, Sathya Lim, Ryo Ishikawa, Takashige Ishii
    AbstractCultivated rice (Oryza sativa L.) was domesticated from the Asian wild species O. rufipogon. Compared with cultivated rice, wild rice has spikelets/seeds with long barbed awns. In order to evaluate the role of awns in wild rice, four seed groups with different awn lengths (full, half, quarter and no awns) were prepared, and the following seed dispersal movements were investigated under simulated natural conditions as observed in the tropical Asian habitat: (1) seed detachment from the parent plant; (2) falling angle of mature seed; (3) ability to slip into small spaces; (4) horizontal movement on the ground and (5) horizontal movement in water. As a result, awns were found to enhance the detachment of matured seeds from the panicles in the initial seed dispersal step. They regulated vertical orientation in the air, and the vertical form may enable seeds to squeeze to the ground. The awned seeds also showed advantages in horizontal movements on the ground and in the water. In most of the experiments, seeds with full awns showed the best performance for seed dispersal, suggesting that wild rice keeps long awns to survive under natural conditions. Since seed awning is dominantly controlled by wild functional alleles at several loci, wild rice may be able to prevent a drastic reduction of awn length.
    Corresponding, Cambridge University Press (CUP), Dec. 2020, Seed Science Research, 30(4) (4), 319 - 324, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Koji Numaguchi, Takashi Akagi, Yuto Kitamura, Ryo Ishikawa, Takashige Ishii
    Domestication and population differentiation in crops involve considerable phenotypic changes. The logs of these evolutionary paths, including natural/artificial selection, can be found in the genomes of the current populations. However, these profiles have been little studied in tree crops, which have specific characters, such as long generation time and clonal propagation, maintaining high levels of heterozygosity. We conducted exon-targeted resequencing of 129 genomes in the genus Prunus, mainly Japanese apricot (Prunus mume), and apricot (Prunus armeniaca), plum (Prunus salicina), and peach (Prunus persica). Based on their genome-wide single-nucleotide polymorphisms merged with published resequencing data of 79 Chinese P. mume cultivars, we inferred complete and ongoing population differentiation in P. mume. Sliding window characterization of the indexes for genetic differentiation identified interspecific fragment introgressions between P. mume and related species (plum and apricot). These regions often exhibited strong selective sweeps formed in the paths of establishment or formation of substructures of P. mume, suggesting that P. mume has frequently imported advantageous genes from other species in the subgenus Prunus as adaptive evolution. These findings shed light on the complicated nature of adaptive evolution in a tree crop that has undergone interspecific exchange of genome fragments with natural/artificial selections.
    Oct. 2020, The Plant Journal, 104(6) (6), 1551 - 1567, English, International magazine
    [Refereed]
    Scientific journal

  • Yaddehige Priya Jayantha Amarasinghe, Rie Kuwata, Akinori Nishimura, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii
    Corresponding, Apr. 2020, RICE, 13(1) (1), 26 - 26, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Numaguchi K, Kitamura Y, Ishikawa R, Ishii T
    Prunus mume Sieb. et Zucc. (conventionally called Japanese apricot, Chinese mei and mume) is a deciduous fruit tree species in the genus Prunus. More than 300 P. mume cultivars are distributed mainly in East Asia, including Japanese fruit, small-fruit and ornamental cultivars and Taiwanese cultivars. In order to estimate demographic history of three Japanese and one Taiwanese subpopulations, approximate Bayesian computation analysis was carried out using 20 SSR genotype datasets of 53 cultivars (20 fruit, 8 small-fruit, 20 ornamental and 5 Taiwanese cultivars). At first, the best fitting model (posterior probability: 0.501) was estimated among six probable scenarios, and median values of demographic parameters were computed. The generation time for P. mume cultivars was assumed to be seven years (full reproductive age) for time conversion. In the best scenario, Japanese and Taiwanese populations had first diverged at 10360 (95% confidence interval (CI): 2079-56910) years ago, followed by the separation of ornamental cultivars among Japanese populations at 3633 (95% CI: 1218-12740) years ago, and final differentiation between fruit and small-fruit cultivars at 2387 (95% CI: 623-6258) years ago. Although the divergence times were roughly estimated, the results suggest that Japanese and Taiwanese populations were separated through the geographic isolation with different climate conditions, and ornamental, fruit and small-fruit cultivars were recently differentiated based on human preference in Japan.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2020, Journal of Crop Research, 65, 31 - 35, English
    [Refereed]
    Scientific journal

  • Rieko Shishido, Masahiro Akimoto, Tin Htut, Tadashi Sato, Takashige Ishii
    Sep. 2019, Breeding Science, 69(3) (3), 471 - 477, English, Domestic magazine
    [Refereed]
    Scientific journal

  • Funa Ogawa, Ryo Ishikawa, Takashige Ishii
    In rice, spikelet opening is an important process for anthesis to produce seeds. Spikelet opening is greatly affected by temperature, humidity, light, and physical stress. To clarify the important factors for spikelet opening, flowering panicles of Japonica cultivar Nipponbare were observed under four controlled transpiration conditions designated as submergence, shower, oil and high-humidity conditions. The submergence condition was designed to allow transpiration only from panicles. Other three conditions were designed to suppress transpiration from panicles by water, oil and high humidity. For each condition, four panicles with 15 spikelets were prepared, and flowering ratios were recorded on the following afternoon. The examination was repeated for three days, and the averages were compared. Under the submergence condition, many flowered spikelets were observed with the average value of 83.3%. On the other hand, a few or no spikelets flowered in the panicles under the shower (17.8%) and oil conditions (0.6%). Almost half of the spikelets (46.1%) flowered under the high-humidity condition. These average values were all significantly lower than the controls (ca. 92%) without any treatments. These results indicate that transpiration from lemma and palea is necessary to have spikelet opening. In addition, spikelet opening time was observed using a wild accession of O. rufipogon to examine whether transpiration from an awn (a tip organ of the lemma) is associated with flowering time or not. Eight panicles of three wild plants were prepared, and awns of the spikelets were alternatively cut based on the primary branch order. The plants were put outside under the sun, and the exact opening time was recorded in minutes for all the spikelets flowered on the following morning. As a result, average opening times for awned and awnless spikelets were not significantly different to each other. Since opening time was not associated with transpiration from the awns, a precise switch for panicle opening may synchronize with transpiration from the surface of the lemma and/or palea.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2019, Journal of Crop Research, 64, 31 - 35, English
    [Refereed]
    Scientific journal

  • Yaddehige P, J. Amarasinghe, Rie Kuwata, Ryo Ishikawa, Takashige Ishii
    Long seed awning in wild rice is one of the propagation-related traits in natural habitats. During rice domestication, plants with shorter awns may gradually be selected because long awns disturb seed harvesting and handling. To clarify the domestication process of awnless rice, we first produced 146 backcross recombinant inbred lines at BC2F7 generation between Oryza rufipogon W630 (a recurrent parent) and O. sativa IR36 (a donor parent). Of these, a single line of G5 having shortest awns was selected. The G5 backcross recombinant inbred line was found to have IR36 homozygous alleles at 20 out of 167 SSR marker loci, showing seven IR36 chromosomal segments in the wild genetic background. The G5 line was further crossed with W630, and 160 BC3F2 progenies were generated. They were planted in the paddy field, and QTL analysis for awnlessness was carried out using awn length data at 5th spikelet position. As a result, one minor QTL and two major QTLs were detected on chromosomes 2 and 4, respectively. According to the associated marker positions, two major QTLs on chromosome 4 seem to be identical to An-1 and LABA1 loci, and the QTL on chromosome 2 may be a novel locus. Although these QTLs explained most of the awn length difference between O. rufipogon W630 and the G5 line, some other minor loci for awnlessness may still exist in the genome of awnless IR36.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2019, Journal of Crop Research, 64, 43 - 46, English
    [Refereed]
    Scientific journal

  • Koji Numaguchi, Shogo Ishio, Yuto Kitamura, Kentaro Nakamura, Ryo Ishikawa, Takashige Ishii
    Japanese Society for Horticultural Science, 2019, The Horticulture Journal, 88(2) (2), 222 - 231, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yuki Tsujimura, Shohei Sugiyama, Kotaro Otsuka, Than Myint Htun, Koji Numaguchi, Cristina Castillo, Takashi Akagi, Takashige Ishii, Ryo Ishikawa
    Springer Science and Business Media LLC, 2019, Theor. Appl. Genet., 132(9) (9), 2615 - 2623, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Takashige Ishii, Ryo Ishikawa
    Springer Singapore, Mar. 2018, Rice Genomics, Genetics and Breeding, 207 - 221, English
    [Refereed]
    In book

  • Ryo Ishikawa, Masahide Iwata, Kenta Taniko, Gotaro Monden, Naoya Miyazaki, Chhourn Orn, Yuki Tsujimura, Shusaku Yoshida, Jian Feng Ma, Takashige Ishii
    Oct. 2017, PLOS ONE, 12(10) (10), e0187224, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryo Ishikawa, Akinori Nishimura, Than Myint Htun, Ryo Nishioka, Yumi Oka, Yuki Tsujimura, Chizuru Inoue, Takashige Ishii
    Apr. 2017, GENETICA, 145(2) (2), 201 - 207, English
    [Refereed]
    Scientific journal

  • Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii
    Corresponding, Feb. 2017, GENES & GENETIC SYSTEMS, 92(1) (1), 21 - 26, English
    [Refereed]
    Scientific journal

  • Yuki Tsujimura, Chizuru Inoue, Than Mynt Htun, Yumi Oka, Takashige Ishii, Ryo Ishikawa
    Asian cultivated rice Oryza sativa L. has been known to be domesticated from wild rice, O. rufipogon. During domestication, agronomically suitable traits were selected, including a loss of seed shattering. In previous studies, three quantitative trait loci (QTLs), sh4, qSH1, and qSH3, were found to be involved in the non-shattering behaviour of O. sativa Japonica ‘Nipponbare’. In contrast, O. sativa Indica ‘IR36’ has cultivated alleles at sh4 and qSH3, but a partial abscission layer formation has been observed. Differences in the seed-shattering degree between Japonica and Indica rice are mainly accounted for by the genotypes at qSH1. However, the allele effects at the loci controlling the non-shattering behaviour of Indica rice are not known clearly. We previously produced an introgression line (IL) carrying O. sativa Japonica ‘Nipponbare’ cultivated alleles at sh4 and qSH3 in the genetic background of wild rice O. rufipogon W630. Although this introgression line (hereafter IL(3+4)) has the same genotypes at sh4 and qSH3 as O. sativa Indica ‘IR36’, it has a well-formed abscission layer compared to that of IR36, resulting in very easy-shattering like wild rice. To understand the independent effects of sh4 and qSH3 on non-shattering behaviour of ‘IR36’, we evaluated the F1 and F2 plants between ‘IR36’ and IL(3+4). In F1 plants, the formation of a clear abscission layer was observed in a manner similar to that of IL(3+4), suggesting that ‘IR36’ may harbour recessive allele(s) at unidentified seed-shattering loci. We also observed the segregation of seed-shattering behaviour in the F2 population, confirming that other unknown mutation(s) may underlie the non-shattering behaviour of ‘IR36’.
    The Society of Crop Science and Breeding in Kinki, Japan, 2017, Journal of Crop Research, 62, 19 - 23, English
    [Refereed]
    Scientific journal

  • Ryo Ishikawa, Takafumi Watabe, Ryo Nishioka, Pham Tien Thanh, Takashige Ishii
    Differences in floral morphologies affect pollination behaviour in many flowering plants. In the genus Oryza, several differences in the size of floral organs are known. In this study, we focused on the differences in the size of floral organs between common cultivated rice, Oryza sativa L. and its wild ancestor, O. rufipogon. We compared floral morphologies between cultivated rice O. sativa cv. Nipponbare and O. rufipogon W630. We first evaluated temporal changes in filament and anther lengths. W630 had longer filaments with rapid elongation within 15 min after spikelet opening. W630 also had longer anthers than Nipponbare, and size of anther was consistent throughout all time examined. We also analysed other six floral traits, and found that W630 had higher stigma and style length, as well as lemma and palea length, but lower lemma and palea width. Quantitative trait locus (QTL) analysis was performed to identify the loci controlling these floral traits, using backcross recombinant inbred lines derived from a cross between Nipponbare and W630. A total of 11 significant QTLs were identified. Of these, two pairs of QTLs for lemma and palea length and one pair for lemma and palea width overlapped, suggesting that common genetic factors may be the reason for the differences in these traits. In addition, we performed QTL analysis for grain size, and found that QTLs for grain size coincided with those for lemma and palea size, indicating that grain size is partly controlled by glume capacity. The QTLs identified in this study will be informative for understanding genetic changes associated with rice domestication.
    Scientific Research Publishing, 2017, American Journal of Plant Science, 8, 734 - 744, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryo Nishioka, Hiromi Mikazuki, Than Myint Htun, Ryo Ishikawa, Takashige Ishii
    Rice (Oryza sativa L.) is derived from the Asian common wild rice, O. rufipogon. In the early stage of domestication, panicle shape may be a key trait before the emergence of plants with non-seed shattering, because wild plants with cultivated-like type of closed panicles were observed to reduce seed shattering through seed retention. Previously, we identified a major locus for this trait to be SPR3 on chromosome 4, controlling gene expression of OsLG1. However, we also found some experimental lines, i.e., BC2F8 backcross recombinant inbred lines between O. sativa Nipponbare and O. rufipogon W630, that showed open panicles without wild alleles at SPR3. In this study, a line of AsN136 was selected from them in order to survey new genomic regions responsible for panicle spreading in wild rice. The AsN136 had wild homozygous alleles at 27 out of 180 SSR marker loci, showing nine wild chromosomal segments in the genetic background of Nipponbare. This line was crossed with Nipponbare, and the segregating population consisted of 150 BC3F2 plants were produced. They were planted in the paddy field, and their panicle shape was examined based on the observation of the rachisbranch junctions. QTL analysis for panicle shape was carried out with their marker genotype data at 27 SSR loci. As a result, two putative loci were detected on chromosomes 5 and 11, explaining 37.6% and 4.9% of the total phenotypic variance, respectively. The putative locus on chromosome 5 with stronger effect was further examined by the progeny test using BC3F3 plants. The results indicated that a new factor for panicle shape was located in the region of RM421-RM274. Expression analysis on OsLG1 and fine mapping of the new locus on chromosome 5 may give good clues to clarify the gene mechanism of panicle spreading.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2016, Journal of Crop Research, 61, 23 - 26, English
    [Refereed]
    Scientific journal

  • Mai Ikemoto, Tetsuya Ohyagi, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii
    Annual and perennial differentiation in rice has been one of the major concerns for rice geneticists and ecologists. Among the traits related to reproductive systems, shooting and rooting ability from the nodes and ratooning ability were focused in this study. They were evaluated using backcross recombinant inbred lines (BRILs) between a perennial cultivar of Oryza sativa Nipponbare and an annual accession of O. rufipogon W630. Firstly, five pairs of first and second nodes from the top were collected from each BRIL plant before and after maturing stages. They were half-dipped in water for three weeks and shoot and root generation was observed. From the first nodes, few BRIL plants produced shoots at both stages, whereas most of them generated shoots from the second nodes. Rooting ability was not well observed for the first nodes of the BRILs after maturing, but almost half of their first nodes produced roots before maturing. From the second nodes, many BRILs generated roots at both stages. Continuous frequency distributions in the BRILs were observed for the shooting and rooting ability from the second nodes after maturing. Based on these data, QTL analysis was carried out, however, no significant loci were detected. Environmental factors such as water temperature might affect the evaluation of the traits. Secondly, ratooning ability was examined with another set of BRILs. About 60 days after heading, the percentages of ratoon numbers relative to total number of productive tillers were examined in the field. They were used for QTL analysis for ratooning ability, and one QTL was detected in the region of RM25-RM44 on chromosome 8 with LOD score of 4.2. Chromosome segment substitution analysis on the putative region will be needed to confirm the QTL location.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2016, Journal of Crop Research, 61, 13 - 17, English
    [Refereed]
    Scientific journal

  • Chhourn Orn, Rieko Shishido, Masahiro Akimoto, Ryo Ishikawa, Than Myint Htun, Ken-Ichi Nonomura, Yohei Koide, Men Sarom, Seng Vang, Sakhan Sophany, Ouk Makara, Takashige Ishii
    Corresponding, Dec. 2015, BREEDING SCIENCE, 65(5) (5), 430 - 437, English
    [Refereed]
    Scientific journal

  • Hao Yin, Masahiro Akimoto, Reunreudee Kaewcheenchai, Masahiro Sotowa, Takashige Ishii, Ryuji Ishikawa
    Oct. 2015, GENES & GENETIC SYSTEMS, 90(5) (5), 269 - 281, English
    [Refereed]
    Scientific journal

  • Inhibition of abscission layer formation by an interaction of two seed-shattering loci, sh4 and qSH3, in rice
    Chizuru Inoue, Than Myint Htun, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa
    Feb. 2015, GENES & GENETIC SYSTEMS, 90(1) (1), 1 - 9, English
    [Refereed]
    Scientific journal

  • Identification of QTL for Striga hermonthica resistance using backcross population derived from a cross between Oryza sativa (cv. Nipponbare) and O. rufipogon
    Atera AE, Onyango JC, Thanh PT, ISHII TAKASHIGE, Itoh K
    2015, J Agr Sci, 7, 99 - 105, English
    [Refereed]
    Scientific journal

  • Aloka Lanka Ranawake, Oliver Escano Manangkil, Shinya Yoshida, Takashi Ishii, Naoki Mori, Chiharu Nakamura
    Nov. 2014, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 28(6) (6), 989 - 998, English
    [Refereed]
    Scientific journal

  • Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, Ryo Ishikawa
    Sep. 2014, BREEDING SCIENCE, 64(3) (3), 199 - 205, English
    [Refereed]
    Scientific journal

  • Orn Chhourn, Ryo Ishikawa, Sakhan Sophany, Ouk Makara, Takashige Ishii
    Genetic variation among rice varieties in Cambodia was evaluated using 276 local and 60 modern varieties. The local varieties were collected more than 50 years ago, and they were classified into four groups (floating rice, rice in dry regions, common non-glutinous rice, glutinous rice). The modern varieties consisted of 35 domestic and 25 foreign lines. Their total DNA samples were extracted and analysed with 12 SSR markers across 12 rice chromosomes. Based on the gene diversity values, genetic variation within group was investigated. Similar levels of genetic variation were observed in four local variety groups, and the overall gene diversity value for 276 local varieties was 0.55. As for modern varieties, lower gene diversity value (0.46) was observed for 35 modern domestic varieties that were generated in Cambodia. The decrease of genetic variation in Cambodianorigin modern varieties may be due to the genetic reduction by modern plant breeding and the social condition changes. However, the overall gene diversity in 60 modern varieties (including 25 foreign varieties) was 0.61, indicating that the high level of genetic variation in Cambodian modern varieties has been maintained by the heterogeneous composition of domestic and foreign varieties. Genetic differentiation among four local and one domestic modern groups were evaluated based on Nei's genetic distances. The lowest value (0.09) was detected between non-glutinous rice and modern domestic groups, suggesting that modern domestic lines were mainly originated from local non-glutinous varieties. High genetic distance values (0.32-0.51) were observed between floating rice and other four. This suggests that deep-water ecosystem might have enhanced the specific diversification of floating rice varieties.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2014, Journal of Crop research, 59, 37 - 41, English
    [Refereed]
    Scientific journal

  • Jeffrey A. Fawcett, Tomoyuki Kado, Eriko Sasaki, Shohei Takuno, Kentaro Yoshida, Ryuichi P. Sugino, Shunichi Kosugi, Satoshi Natsume, Chikako Mitsuoka, Aiko Uemura, Hiroki Takagi, Akira Abe, Takashige Ishii, Ryohei Terauchi, Hideki Innan
    Dec. 2013, PLOS ONE, 8(12) (12), e83720 (12 pages), English
    [Refereed]
    Scientific journal

  • CONSTRUCTION OF MICROSATELLITE LINKAGE MAP AND DETECTION OF SEGREGATION DISTORTION IN INDICA RICE (ORYZA SATIVA L.)
    Muhammad Arshad Javed, Fahrul Zaman Huyop, Takashige Ishii, Azman Abd Samad, Tariq Mahmood, Muhammad Saleem Haider, Muhammad Saleem
    Dec. 2013, PAKISTAN JOURNAL OF BOTANY, 45(6) (6), 2085 - 2092, English
    [Refereed]
    Scientific journal

  • Takashige Ishii, Koji Numaguchi, Kotaro Miura, Kentaro Yoshida, Pham Thien Thanh, Than Myint Htun, Masanori Yamasaki, Norio Komeda, Takashi Matsumoto, Ryohei Terauchi, Ryo Ishikawa, Motoyuki Ashikari
    Apr. 2013, NATURE GENETICS, 45(4) (4), 462 - 465, English
    [Refereed]
    Scientific journal

  • Phan Thi Thanh, Cristian Ioan Vladutu, Shahryar F. Kianian, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Naoki Mori
    Apr. 2013, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 27(2) (2), 3627 - 3637, English
    [Refereed]
    Scientific journal

  • Striga infestation in Kenya: Status, distribution and management options
    Atera AE, Ishii T, Onyango JC, Itoh K, Azuma T
    2013, Sust Agr Res, 2, 99 - 108, English
    [Refereed]
    Scientific journal

  • Phuong Dang Thai Phan, Hiroki Kageyama, Ryo Ishikawa, Takashige Ishii
    Corresponding, Sep. 2012, BREEDING SCIENCE, 62(3) (3), 256 - 262, English
    [Refereed]
    Scientific journal

  • Evans Atuti Atera, Kazuyuki Itoh, Tetsushi Azuma, Takashige Ishii
    Mar. 2012, WEED BIOLOGY AND MANAGEMENT, 12(1) (1), 53 - 62, English
    [Refereed]
    Scientific journal

  • Kazuyuki Mori, Nobuhiro Mukojima, Takashi Nakao, Seiji Tamiya, Yu Sakamoto, Norio Sohbaru, Kazuya Hayashi, Hitomi Watanuki, Kazuhiro Nara, Kaoru Yamazaki, Takashige Ishii, Kazuyoshi Hosaka
    Feb. 2012, AMERICAN JOURNAL OF POTATO RESEARCH, 89(1) (1), 63 - 72, English
    [Refereed]
    Scientific journal

  • Response of NERICA Rice to Striga hermonthica Infections in Western Kenya
    Evans A. Atera, Kazuyuki Itoh, Tetsushi Azuma, Takashige Ishii
    2012, INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, 14(2) (2), 271 - 275, English
    [Refereed]
    Scientific journal

  • Farmers’ perception and constraints to the adoption of weed control options: the case of Striga asiatica in Malawi
    Atera AE, ITOH KAZUYUKI, AZUMA TETSUSHI, ISHII TAKASHIGE
    2012, J Agr Sci, 4, 41 - 50, English
    [Refereed]
    Scientific journal

  • Takashige Ishii, Takashi Hiraoka, Tomoyuki Kanzaki, Masahiro Akimoto, Rieko Shishido, Ryo Ishikawa
    Dec. 2011, RICE, 4(3-4) (3-4), 170 - 177, English
    [Refereed]
    Scientific journal

  • Kazuyuki Mori, Yu Sakamoto, Nobuhiro Mukojima, Seiji Tamiya, Takashi Nakao, Takashige Ishii, Kazuyoshi Hosaka
    Aug. 2011, EUPHYTICA, 180(3) (3), 347 - 355, English
    [Refereed]
    Scientific journal

  • Pham Thien Thanh, Phuong Dang Thai Phan, Naoki Mori, Ryo Ishikawa, Takashige Ishii
    Corresponding, Mar. 2011, BREEDING SCIENCE, 61(1) (1), 76 - 79, English
    [Refereed]
    Scientific journal

  • Than Myint Htun, Takashige Ishii, Ryo Ishikawa
    Seed shattering is important for wild plant species to guarantee efficient seed propagation. In rice, two shattering loci, sh4 and qSH1, with strong effects on seed shattering were previously identified through the map-based approach. In this study, a rice cultivar, Oryza sativa Japonica cv. Nipponbare, and an annual wild accession, O. rufipogon W630 were used as plant materials. The former contains non-functional alleles at sh4 and qSH1, whereas the latter has shattering alleles at both loci. Since the ancient humans selected non-shattering mutants in the past, it is important to evaluate shattering behavior of plants carrying non-functional alleles in the genetic background of wild rice. Therefore, O. sativa cv. Nipponbare was backcrossed twice with O. rufipogon, and backcross plants at BC2 generation were produced. Among them, the substitution plants having both cultivated alleles at sh4 and qSH1 loci in the genetic background of wild rice were selected by dCAPS and microsatellite markers. Using these lines and the parents, the temporal changes of shattering degree after flowering were examined based on the breaking tensile strength values for detachment of seeds from the pedicels. At 5 days after flowering, the shattering degrees of the substitution lines were almost similar to that of wild accession. They decreased moderately but maintained around half values of cultivated Nipponbare at 10 days after pollination. Probably, partial formation of abscission layers in pedicels might allow matured seeds to be kept on the panicles. These results suggest that the wild rice may still keep additional gene(s) promoting the formation of abscission layer besides qSH1 and sh4 alleles.
    The Society of Crop Science and Breeding in Kinki, Japan, 2011, Journal of Crop research, 56, 39 - 44, English
    [Refereed]
    Scientific journal

  • Phuong Dang, Thai Phan, Pham Thien Thanh, Naoki Mori, Ryo Ishikawa, Takashige Ishii
    Backcross populations are useful for evaluating the agronomic traits from unadapted germplasms. In this study, we produced 159 and 170 backcross recombinant inbred lines (BRILs) at the BC2F8 generation between Oryza sativa Japonica Nipponbare (a recurrent parent) and O. rufipogon W630 and between O. sativa Indica IR36 (a recurrent parent) and O. rufipogon W630, respectively. These BRILs were found to have nearly homozygous genome constitutions based on the marker genotypes at SSR loci covering whole chromosomes. Since their selfed progenies have almost identical genotypes in each line, the simple test tube method was applied for BRILs (BC2F9 generation) to evaluate the ability of fast shoot elongation after germination stage under submerged condition. Then, QTL analysis of submergence avoidance was carried out with marker genotype data. As a result, three (on chromosomes 2, 3 and 10) and two QTLs (on chromosomes 3 and 4) were found in the BRILs having genetic backgrounds of Nipponbare and IR36, respectively. Of these, one each had the wild allele with increasing effect on shoot length. These favorable wild alleles would be useful in improving direct-seeding cultivation in water-prone areas.
    Corresponding, The Society of Crop Science and Breeding in Kinki, Japan, 2011, Journal of Crop research, 56, 45 - 49, English
    [Refereed]
    Scientific journal

  • QTL analysis for flowering time using backcross population between Oryza sativa Nipponbare and O. rufipogon
    Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii
    Corresponding, Aug. 2010, GENES & GENETIC SYSTEMS, 85(4) (4), 273 - 279, English
    [Refereed]
    Scientific journal

  • Allelic interaction at seed-shattering loci in the genetic backgrounds of wild and cultivated rice species
    Ryo Ishikawa, Pham Thien Thanh, Naoto Nimura, Than Myint Htun, Masanori Yamasaki, Takashige Ishii
    Corresponding, Aug. 2010, GENES & GENETIC SYSTEMS, 85(4) (4), 265 - 271, English
    [Refereed]
    Scientific journal

  • Genetic diversity and origin of timopheevi wheat inferred by chloroplast DNA fingerprinting
    Naoki Mori, Yasutaka Kondo, Takashige Ishii, Taihachi Kawahara, Jan Valkoun, Chiharu Nakamura
    Dec. 2009, BREEDING SCIENCE, 59(5) (5), 571 - 578, English
    [Refereed]
    Scientific journal

  • Evaluation of genetic diversity among wild rice populations of Oryza rufipogon in Myanmar and Cambodia
    Kageyama H, Akimoto M, Shishido R, Sein T, Htut T, Sarom M, Sato T, ISHII TAKASHIGE
    Corresponding, 近畿作物・育種研究会, 2009, Journal of Crop Research, 54(54) (54), 47 - 51, English
    [Refereed]
    Scientific journal

  • Evaluation of genetic diversity among rice local varieties in Vietnam
    Takashi Hiraoka, Nobuyuki Ikeda, Ryo Ishikawa, Takashige Ishii
    Corresponding, 近畿作物・育種研究会, 2009, Journal of Crop Research, 54(54) (54), 125 - 130, English
    [Refereed]
    Scientific journal

  • Mapping of quantitative trait loci associated with cold tolerance at the post-germination stage in rice
    A. L. Ranawake, T. Ishii, N. Mori, S. Yoshida, C. Nakamura
    Feb. 2008, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 22(1) (1), 536 - 540, English
    [Refereed]
    Scientific journal

  • The role of alternating culture temperatures and maltose in enhancing the anther culture efficiency of salt tolerant indica rice (Oryza sativa L.) cultivars, Pokkali and Nona Bokra
    Muhammad A. Javed, Takashige Ishii, Osamu Kamijima, Shuji Misoo
    Jun. 2007, PLANT BIOTECHNOLOGY, 24(3) (3), 283 - 287, English
    [Refereed]
    Scientific journal

  • Sujay Rakshit, Arunita Rakshit, Hideo Matsumura, Yoshihiro Takahashi, Yoshitaka Hasegawa, Akiko Ito, Takashige Ishii, Naohiko T. Miyashita, Ryohei Terauchi
    Feb. 2007, THEORETICAL AND APPLIED GENETICS, 114(4) (4), 731 - 743, English
    [Refereed]
    Scientific journal

  • Noriaki Aoki, Takayuki Umemoto, Shinya Yoshida, Takashige Ishii, Osamu Kamijima, Ushio Matsukura, Naoyoshi Inouchi
    Sep. 2006, EUPHYTICA, 151(2) (2), 225 - 234, English
    [Refereed]
    Scientific journal

  • Mitochondrial microsatellite variability in common wheat and its ancestral species
    Takashige Ishii, Chisa Takahashi, Nobuyuki Ikeda, Osamu Kamijima, Naoki Mori
    Jun. 2006, GENES & GENETIC SYSTEMS, 81(3) (3), 211 - 214, English
    [Refereed]
    Scientific journal

  • 野生イネの種子脱粒性、穂の開帳性、頴の色を支配する遺伝子のマッピング
    ISHII TAKASHIGE, 崎山 弘樹, ISHIKAWA RYO, 上島 脩志
    2006, 近畿作物・育種研究, 51, 23 - 26, Japanese
    [Refereed]
    Scientific journal

  • Discrepancy of two ecotypes of Oryza sativa L. to salinity at germination and seedling stage
    Javed MA, ISHII TAKASHIGE, Kamijima O, Misoo S
    2006, Annals Biol, 22, 201 - 211, English
    [Refereed]
    Scientific journal

  • Mechanism of high photosynthetic capacity in BC2F4 lines derived from a cross between Oryza sativa and wild relatives O-rufipogon
    C Masumoto, T Ishii, T Hatanaka, N Uchida
    Dec. 2005, PLANT PRODUCTION SCIENCE, 8(5) (5), 539 - 545, English
    [Refereed]
    Scientific journal

  • DNA variation in the metallothionein genes in wild rice Oryza rufipogon: Relationship between DNA sequence polymorphism, codon bias and gene expression
    NT Miyashita, K Yoshida, T Ishii
    Jun. 2005, GENES & GENETIC SYSTEMS, 80(3) (3), 173 - 183, English
    [Refereed]
    Scientific journal

  • Development of near isogenic lines targeting wild QTL from Oryza rufipogon in the genetic cackground of O.sativa by marker-assisted selection and backcrossing.
    IKEDA N, KAMIJIMA Osamu, ISHII Takashige
    Corresponding, Kobe University, 2005, Mem. Grad. Sci.Technol.,Kobe Univ., 23-A:37-49, 37 - 49, English
    [Refereed]
    Research institution

  • C Vitte, T Ishii, F Lamy, D Brar, O Panaud
    Dec. 2004, MOLECULAR GENETICS AND GENOMICS, 272(5) (5), 504 - 511, English
    [Refereed]
    Scientific journal

  • K Yoshida, NT Miyashita, T Ishii
    Nov. 2004, THEORETICAL AND APPLIED GENETICS, 109(7) (7), 1406 - 1416, English
    [Refereed]
    Scientific journal

  • Z Hashimoto, N Mori, M Kawamura, T Ishii, S Yoshida, M Ikegami, S Takumi, C Nakamura
    Nov. 2004, THEORETICAL AND APPLIED GENETICS, 109(8) (8), 1586 - 1596, English
    [Refereed]
    Scientific journal

  • Chloroplast and nuclear DNA variation in common wheat: insight into the origin and evolution of common wheat
    S Hirosawa, S Takumi, T Ishii, T Kawahara, C Nakamura, N Mori
    Oct. 2004, GENES & GENETIC SYSTEMS, 79(5) (5), 271 - 282, English
    [Refereed]
    Scientific journal

  • Enhancement of rice leaf photosynthesis by crossing between cultivated rice, Oryza sativa and wild rice species, Oryza ryfipogon
    C Masumoto, T Ishii, S Kataoka, T Hatanaka, N Uchida
    Sep. 2004, PLANT PRODUCTION SCIENCE, 7(3) (3), 252 - 259, English
    [Refereed]
    Scientific journal

  • Wild-QLT-allele effect in the background of Japonica (Nipponbare) and Indica (IR36) cultivars.
    ISHII Takashige, BAUTISTA N S, SHIMADZUTSU K, KOBAYASHI N, UCHIDA Naotsugu, KAMIJIMA Osamu
    IRRI, 2003, Advances in Rice Genetics, pp.133-135, English
    [Refereed]
    In book

  • Trait-improving wild QTL alleles identified using advanced backcross QLT analysis from a cross between cultivated rice, Oryza sativa and wild rice, O. rufipogon.
    BAUTISTA N S, SHIMADZUTSU K, TERANISHI T, TAKAMATSU S, KOBAYASHI N, UCHIDA Naotsugu, KAMIJIMA Osamu, ISHII Takashige
    Corresponding, 2003, Advances in Rice Genetics, pp.135-138, English
    [Refereed]
    In book

  • Salinity tolerance of three Basmati rice lines: A comparative study of yield related characters.
    JAVED M. A, SHABBIR G, MISOO Shuji, ISHII Takashige, KAMIJIMA Osamu
    2003, Kinki J. Crop Sci. Breed., 48,pp.7-12., English
    [Refereed]
    Scientific journal

  • Marker-assisted evaluation of germplasm resources for plant breeding
    Xu Y, ISHII TAKASHIGE, McCouch SR
    International Rice Research Institute, 2003, Rice Science: Innovations and Impact for Livelihood, 213 - 229, English
    [Refereed]
    In book

  • QTL analysis for plant and grain characters of sake-brewing rice using a doubled haploid population
    S Yoshida, M Ikegami, J Kuze, K Sawada, Z Hashimoto, T Ishii, C Nakamura, O Kamijima
    Dec. 2002, BREEDING SCIENCE, 52(4) (4), 309 - 317, English
    [Refereed]
    Scientific journal

  • N Asakura, C Nakamura, T Ishii, Y Kasai, S Yoshida
    Nov. 2002, MOLECULAR GENETICS AND GENOMICS, 268(3) (3), 321 - 330, English
    [Refereed]
    Scientific journal

  • 農業形質から評価した在来アズキ系統の遺伝的多様性
    伊勢村武久, 石井尊生, 三十尾修司, 上島脩志
    神戸大学, 2002, 神戸大学大学院自然科学研究科紀要, 20-B, 165 - 176, Japanese
    Research institution

  • 伊勢村武久, 石井尊生, 斎藤大樹, 野田千代, 三十尾修司, 上島脩志
    RAPD analysis was carried out to elucidate the genetic variation of 203 azuki bean landraces from Japan, South Korea, China, Taiwan, Bhutan and Nepal. In total, 413 distinct bands were amplified using 48 primers. Of these, 97 bands were found to be polymorphic. Most of the landraces could be identified individually using these RAPD markers. Based on a simple matching coefficient using 97 polymorphic bands, average dissimilarities of landraces between regions were larger than those within regions. Particularly, large genetic differences were observed between the landraces in the Bhutan-Nepal region and those in Japan (0.615), and between the Bhutan-Nepal and Korea-China-Taiwan groups (0.598). Based on the dissimilarities between the landraces, cluster analysis was carried out by the UPGMA method, and 203 landraces were mainly classified into the groups that reflected the collection origin. These results suggested that azuki bean seeds had not been frequently exchanged between the regions in the past. In this study, the following conclusions were reached: Large genetic variation among landraces can be applied for the azuki bean breeding by expanding the genetic base of cultivars. Molecular linkage map can be constructed with the intraspecific populations derived from the cross between the landraces in the Bhutan-Nepal and East Asia regions.
    Japanese Society of Breeding, 2002, 育種学研究, 4(3) (3), 125 - 135, Japanese
    [Refereed]
    Research institution

  • Construction of molecular linkage map between Oryza sativa and O. rufipogon, using microsatellite markers
    Ishii T, Takamatsu S, Ito H, Ikeda N, Isemura T, Kamijima O
    2002, Rice Genet Newsl, 19, 97 - 100, English
    [Refereed]
    Scientific journal

  • Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species
    T Ishii, N Mori, Y Ogihara
    Nov. 2001, THEORETICAL AND APPLIED GENETICS, 103(6-7) (6-7), 896 - 904, English
    [Refereed]
    Scientific journal

  • Nuclear- and chloroplast-microsatellite variation in A-genome species of rice
    T Ishii, Y Xu, McCouch, SR
    Aug. 2001, GENOME, 44(4) (4), 658 - 666, English
    [Refereed]
    Scientific journal

  • RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice
    NS Bautista, R Solis, O Kamijima, T Ishii
    Corresponding, Apr. 2001, GENES & GENETIC SYSTEMS, 76(2) (2), 71 - 79, English
    [Refereed]
    Scientific journal

  • Ultra-simple DNA extraction method for marker-assisted selection using microsatellite markers in rice
    N Ikeda, NS Bautista, T Yamada, O Kamijima, T Ishii
    Corresponding, Mar. 2001, PLANT MOLECULAR BIOLOGY REPORTER, 19(1) (1), 27 - 32, English
    [Refereed]
    Scientific journal

  • Assignment of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph4 to the rice chromosome 6
    M Kawaguchi, K Murata, T Ishii, S Takumi, N Mori, C Nakamura
    Mar. 2001, BREEDING SCIENCE, 51(1) (1), 13 - 18, English
    [Refereed]
    Scientific journal

  • Microsatellites and microsynteny in the chloroplast genomes of Oryza and eight other Gramineae species
    T Ishii, McCouch, SR
    Jun. 2000, THEORETICAL AND APPLIED GENETICS, 100(8) (8), 1257 - 1266, English
    [Refereed]
    Scientific journal

  • Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.)
    S Temnykh, WD Park, N Ayres, S Cartinhour, N Hauck, L Lipovich, YG Cho, T Ishii, McCouch, SR
    Mar. 2000, THEORETICAL AND APPLIED GENETICS, 100(5) (5), 697 - 712, English
    [Refereed]
    Scientific journal

  • Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.)
    YG Cho, T Ishii, S Temnykh, Chen, X, L Lipovich, McCouch, SR, WD Park, N Ayres, S Cartinhour
    Mar. 2000, THEORETICAL AND APPLIED GENETICS, 100(5) (5), 713 - 722, English
    [Refereed]
    Scientific journal

  • Comparative molecular mapping in Ceratotropis species using an interspecific cross between azuki bean (Vigna angularis) and rice bean (V-umbellata)
    A Kaga, T Ishii, K Tsukimoto, E Tokoro, O Kamijima
    Jan. 2000, THEORETICAL AND APPLIED GENETICS, 100(2) (2), 207 - 213, English
    [Refereed]
    Scientific journal

  • Mitochondrial genome differentiation in the genus Phyllostachys
    F Taguchi-Shiobara, T Ishii, T Terachi, K Tsunewaki
    Jan. 1998, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 32(1) (1), 7 - 14, English
    Scientific journal

  • Hideo Maeda, Takashige Ishii, Hirokazu Mori, Junji Kuroda, Masato Horimoto, Itsuro Takamure, Toshiro Kinoshita, Osamu Kamijima
    Dec. 1997, Breeding Science, 47(4) (4), 317 - 320
    Scientific journal

  • Susan R. McCouch, Xiuli Chen, Olivier Panaud, Svetlana Temnykh, Yunbi Xu, Yong Gu Cho, Ning Huang, Takashige Ishii, Matthew Blair
    Sep. 1997, Plant Molecular Biology, 35(1-2) (1-2), 89 - 99
    Scientific journal

  • Tetsuya Yamada, Shuji Misoo, Takashige Ishii, Yasuhiro Ito, Keigo Takaoka, Osamu Kamijima
    Sep. 1997, Breeding Science, 47(3) (3), 229 - 236
    Scientific journal

  • Phylogenetic relationships among rice species based on chloroplast genome differentiation
    Ishii T, Tsunewaki K
    1997, Science of the Rice Plant, 3, 48 - 53

  • 近縁野生種を用いたバレイショ体細胞雑種のゲノム構成と戻し交雑世代における染色体数の変異
    山田哲也, 中川久美, 保坂和良, 三十尾修司, 石井尊生, 上島脩志
    1997, 近畿作物・育種研究, 42, 47 - 48

  • バレイショ二倍性半数体間の体細胞雑種系統における細胞質ゲノムの構成
    栗谷由美, 山田哲也, 三十尾修司, 石井尊生, 淡野一郎, 上島脩志
    1997, 近畿作物・育種研究, 42, 49 - 50

  • A. Kaga, M. Ohnishi, T. Ishii, O. Kamijima
    Oct. 1996, Theoretical and Applied Genetics, 93(5-6) (5-6), 658 - 663
    Scientific journal

  • Kuldeep Singh, Takashige Ishii, Arnold Parco, Ning Huang, Darshan S. Brar, Gurdev S. Khush
    Jun. 1996, Proceedings of the National Academy of Sciences of the United States of America, 93(12) (12), 6163 - 6168
    Scientific journal

  • Phylogenetic relationships between cultivated and wild species of rice as revealed by DNA polymorphisms
    Ishii T, Nakano T, Maeda H, Kamijima O, Khush GS
    1996, Rice Genetics III, 367 - 372

  • Centromere mapping and orientation of the cytological, classical, and molecular linkage maps of rice
    Khush GS, Singh K, Ishii T, Parco A, Huang N, Brar DS, Multani DS
    1996, Rice Genetics III, 57 - 75

  • Takashige Ishii, Toshitsugu Nakano, Hideo Maeda, Osamu Kamijima
    1996, Genes and Genetic Systems, 71(4) (4), 195 - 201
    Scientific journal

  • R. Dalmacio, D. S. Brar, T. Ishii, L. A. Sitch, S. S. Virmani, G. S. Khush
    Jan. 1995, Euphytica, 82(3) (3), 221 - 225
    Scientific journal

  • Takashige Ishii, Darshan S. Brar, Gerard Second, Gurdev S. Khush, Koichiro Tsunewaki, Takashige Ishii, Koichiro Tsunewaki
    1995, Japanese Journal of Genetics, 70(5) (5), 643 - 652, English
    Scientific journal

  • T. Ishii, D. S. Brar, D. S. Multani, G. S. Khush
    1994, Genome, 37(2) (2), 217 - 221
    Scientific journal

  • T. Ishii, T. Terachi, N. Mori, K. Tsunewaki
    Mar. 1993, Theoretical and Applied Genetics, 86(1) (1), 88 - 96
    Scientific journal

  • T. Ishii, K. Tsunewaki
    1991, Genome, 34(5) (5), 818 - 826
    Scientific journal

  • CYTOPLASMIC AND NUCLEAR-DNA DIFFERENTIATION IN CULTIVATED RICE SPECIES
    T ISHII, T TERACHI, N MORI, K TSUNEWAKI
    1991, RICE GENETICS II, 655 - 657, English
    International conference proceedings

  • Takashige Ishii, Olivier Panaud, D. S. Brar, G. S. Khush
    Aug. 1990, Plant Molecular Biology Reporter, 8(3) (3), 167 - 171
    Scientific journal

  • Chromosomal location of ribosomal RNA genes in rice by in situ hybridization
    Islam-Faridi MN, Ishii T, Kumar V, Sitch LA, Brar DS
    1990, Rice Genet Newsl, 7, 143 - 144

  • RFLP analysis of rice mitochondrial DNA
    Ishii T, Tsunewaki K
    1989, Rice Genet Newsl, 6, 153 - 155

  • Takashige Ishii, Toru Terachi, Koichiro Tsunewaki
    1988, Japanese Journal of Genetics, 63(6) (6), 523 - 536, English
    Scientific journal

  • Takashige Ishii, Toru Terachi, Koichiro Tsunewaki
    1986, Japanese Journal of Genetics, 61(6) (6), 537 - 541, English
    Scientific journal

■ MISC
  • Ishikawa Ryo, Yamagata Yoshiyuki, Koide Yohei, Ishii Takashige, Ishikawa Ryuji, Akimoto Masahiro, Tsuda Katsutoshi, Fujita Daisuke
    Japanese Society of Breeding, 2023, Breeding Research, advpub, Japanese

  • Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species
    Naoki Mori, Yuko Hidehira, Takashige Ishii, Chiharu Nakamura
    The domestication of wheat and barley was the most important step in the emergence of farming communities that led to the ancient civilizations of Mesopotamia. Several lines of evidence indicate that emmer wheat was the earliest form of domesticated wheat derived from wild emmer wheat. Chloroplast DNA fingerprinting of wild and domesticated emmer wheat revealed that two dis
    2009, Proc. 4th International Conference on Energy Efficiency and Agricultural Engineering, 725 - 729, English

  • The salinity tolerance evaluation of two eco-types of Oryza sativa L. at different growth stages in successive salinity levels.
    Javed M.A, ISHII Takashige, KAMIJIMA Osamu, MISOO Shuji
    2006, 3rd. Int.. Conf. on Sustainable Agriculture for Food, Energy, and Industry, English

  • Photosynthesis, growth and yield of BC2F4 lines derived from Oryza sativa and wild rice species, O. rufipogon.
    Chisato Masumoto, Takashige Ishii, HATANAKA TOMOKO, Naotsugu Uchida
    2004, Proceedings of the 4th International Crop Science Congress, 26, English

  • QTL analysis for several agronomic characters using four BC2 poppulations between wild and cultivated rice species.
    ISHII Takashige
    2003, Proc. Int. Genet.Resour. Work. Oryza., pp.7-10, English

  • Origins of domesticated emmer and common wheat inferred from chloroplast DNA fingerprinting.
    MORI Naoki, ISHII Takashige, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA Y
    Instituto Sperimentale per la Cerealicoltura, 2003, Proc. Xth Int. Wheat Genet. Symp., Vol.1,pp.25-28, 25 - 28, English

  • Variation in chloroplast microsatellite loci among wild and cultivated species of Emmer wheat
    Mori N, Ishii T, Ishido T, Belay G, Takumi S, Kawahara T, Ogihara Y, Nakamura C
    2002, Proc 4th Int Triticeae Symp, English

  • ベトナム・ミヤンマーにおける野生イネの収集
    ISHII TAKASHIGE
    2000, 近畿作物・育種研究, 45, 73 - 76, Japanese

  • RFLP分析に基づくイネ属Aゲノム種の系統関係
    石井尊生
    1994, 東北大学遺伝生態研究センター通信, 26, 1 - 3

  • Aegilops seasii is a possible cytoplasmic donor to Ae. kotschyi and Ae. variabilis
    Siregar UJ, Ishii T, Tsunewaki K
    1988, Proc 7th Int Wheat Genet Symp, 1, 145 - 151

■ Books And Other Publications
  • Information and data on natural introgression
    Takashige ISHII
    Contributor, OECD Environment, Health and Safety Publications, 2021

  • Rice Genomics, Genetics and Breeding - Domestication loci controlling panicle shape, seed shattering and seed awning
    Takashige Ishii, Ryo Ishikawa
    Others, Springer, 2018, English
    Scholarly book

  • 植物学の百科事典
    ISHII TAKASHIGE
    Others, 丸善出版（日本植物学会 編）, Jun. 2016, Japanese
    Dictionary or encycropedia

  • 植物ゲノム科学辞典
    ISHII TAKASHIGE
    Others, 朝倉書店, Apr. 2009, Japanese, 量的形質遺伝子座
    Dictionary or encycropedia

  • ミヤンマーの野生イネ
    石井尊生
    アジア遊学 勉誠出版, 2003

  • 葉緑体ゲノムからみた系統関係
    石井尊生, 常脇恒一郎
    稲学大成 農山漁村文化協会, 1990

■ Lectures, oral presentations, etc.
  • Target capture法に基づく日本と中国のウメの集団構造および系統関係について
    沼口孝司, 赤木剛士, 北村祐人, ISHIKAWA RYO, ISHII TAKASHIGE
    園芸学会 平成30年度秋季大会, Mar. 2019, Japanese, 園芸学会, 明治大学, Domestic conference
    Oral presentation

  • QTL analysis for awnlessness using a backcross population between Oryza rufipogon and O. sativa IR36
    Priya J.A. Yaddehige, Rie Kuwata, Ryo Ishikawa, Takashige Ishii
    近畿作物・育種研究会 第186回例会, Nov. 2018, English, 近畿作物・育種研究会, 京都大学, Domestic conference
    Oral presentation

  • 熱帯アジアにおける栽培イネから野生イネへの遺伝子流動について
    谷尾侑香, ORN Chhourn, 秋本正博, 宍戸理恵子, HTUN Than Myint, PHAN Phuong, Dang Thai, 野々村賢一, 小出陽平, 石川亮, 石井尊生
    育種学研究, Sep. 2018, Japanese

  • 野生イネOryza meridionalisを用いて同定されたイネ種子亜鉛濃度を支配するqGZn9は連鎖した２つの遺伝子座によって制御される
    宮崎直哉, 谷古健太, 岩田将英, 門田剛太郎, Orn Chhourn, 吉田周作, ISHII TAKASHIGE, 馬建鋒, ISHIKAWA RYO
    日本育種学会第134回講演会, Sep. 2018, Japanese, 日本育種学会, 岡山大学, Domestic conference
    Oral presentation

  • 穂の開帳性が栽培イネ(Oryza sativa)の穂形態に与える影響について
    窪田芳生, 西岡諒, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第134回講演会, Sep. 2018, Japanese, 日本育種学会, 岡山大学, Domestic conference
    Poster presentation

  • ジャポニカ型栽培イネ（日本晴）の非脱粒性に関与する新規遺伝子座の離層形成への効果
    杉山昇平, 辻村雄紀, 大塚宏太郎, Than Myint Htun, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第134回講演会, Sep. 2018, Japanese, 日本育種学会, 岡山大学, Domestic conference
    Oral presentation

  • イネ育種における野生イネ利用の可能性
    ISHIKAWA RYO, 宮崎直哉, 杉山昇平, 窪田芳生, 馬建鋒, ISHII TAKASHIGE
    日本育種学会第134回講演会, Sep. 2018, Japanese, 日本育種学会, 岡山大学, Domestic conference
    Nominated symposium

  • Exomeと果実・葉形状にみる日本のウメの多様性
    沼口孝司, 赤木剛士, 北村祐人, 大江孝明, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第134回講演会, Sep. 2018, Japanese, 日本育種学会, 岡山大学, Domestic conference
    Oral presentation

  • 野生イネOryza rufipogon における種子脱粒性遺伝子座間の相互作用
    杉山昇平, Cristina Castillo, 岡佑美, Than Myint Htun, 野々村賢一, ISHII TAKASHIGE, ISHIKAWA RYO
    イネ遺伝学・分子生物学ワークショップ2018, Jul. 2018, Japanese, 国立遺伝学研究所, Domestic conference
    Poster presentation

  • オーストラリア由来の野生イネOryza meridionalis を用いたイネ種子亜鉛濃度を支配する遺伝子座の同定
    宮崎直哉, 谷古健太, 岩田将英, 門田剛太郎, Orn Chhourn, 吉田周作, ISHII TAKASHIGE, 馬建鋒, ISHIKAWA RYO
    イネ遺伝学・分子生物学ワークショップ2018, Jul. 2018, Japanese, 国立遺伝学研究所, Domestic conference
    Poster presentation

  • イネにおける小穂の開花メカニズムの解明に向けて
    小川風和, ISHIKAWA RYO, ISHII TAKASHIGE
    近畿作物・育種研究会 第185回例会, Jun. 2018, Japanese, 近畿作物・育種研究会, 神戸大学, Domestic conference
    Oral presentation

  • ジャポニカ型栽培イネOryza sativa 日本晴の非脱粒性に関与する新規遺伝子座の同定
    辻村雄紀, Than Myint Htun, 沼口孝司, 高間菜摘, 杉山昇平, ISHII TAKASHIGE, ISHIKAWA RYO
    第59回日本植物生理学会年会, Mar. 2018, Japanese, 植物生理学会, 札幌コンベンションセンター, Domestic conference
    Poster presentation

  • 野生イネの繁殖に必須の種子脱粒性に関わる遺伝子座の同定
    辻村雄紀, Than Myint Htun, 沼口孝司, 高間菜摘, 杉山昇平, ISHII TAKASHIGE, ISHIKAWA RYO
    第49回種生物学シンポジウム, Dec. 2017, Japanese, 種生物学会, すかっとランド九頭竜, Domestic conference
    Poster presentation

  • 野生イネOryza rufipogonと栽培イネOryza sativaの交雑系統群を利用した新規Na+ 輸送系遺伝子・変異の探索
    堀内智貴, ISHIKAWA RYO, 田野井慶太朗, 小林奈通子, ISHII TAKASHIGE, 堀江智明
    日本育種学会第132回講演会, Oct. 2017, Japanese, 日本育種学会, 岩手大学, Domestic conference
    Oral presentation

  • ウメ遺伝資源における収穫期および果実形質の多様性
    沼口孝司, 北村祐人, 仲慶晃, 大江孝明, 土田靖久, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第132回講演会, Oct. 2017, Japanese, 日本育種学会, 岩手大学, Domestic conference
    Oral presentation

  • ウメ黒星病抵抗性に関与するQTLの同定に向けて
    沼口孝司, 北村祐人, ISHIKAWA RYO, ISHII TAKASHIGE
    園芸学会 平成29年度秋季大会, Sep. 2017, Japanese, 園芸学会, 酪農学園大学, Domestic conference
    Oral presentation

  • 栽培イネと野生イネの戻し交雑系統群を用いたイネの花器官を支配する遺伝子座の同定
    ISHIKAWA RYO, 渡部 貴史, 西岡 諒, Pham Thien Thanh, ISHII TAKASHIGE
    日本育種学会第131回講演会, Jul. 2017, Japanese, 日本育種学会, 名古屋大学, Domestic conference
    Poster presentation

  • インディカ型栽培イネIR36の非脱粒性を支配する新規遺伝子座の探索
    辻村 雄紀, 井上 千鶴, Than Myint Htun, 西村 彬則, 杉山 昇平, 岡 佑美, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第131回講演会, Jul. 2017, Japanese, 日本育種学会, 名古屋大学, Domestic conference
    Poster presentation

  • 野生イネ（Oryza rufipogon）の遺伝的背景で芒を短くするためには - イネの栽培化における無芒性の検証にむけて –
    池本 麻衣, 大塚 光晴, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第131回講演会, Mar. 2017, Japanese, 日本育種学会, 名古屋大学, Domestic conference
    Oral presentation

  • 栽培イネと野生イネの戻し交雑系統群を用いたイネの花器官を支配する遺伝子座の同定
    ISHIKAWA RYO, 渡部 貴史, 西岡 諒, Pham Thien Thanh, ISHII TAKASHIGE
    日本育種学会第131回講演会, Mar. 2017, Japanese, 日本育種学会, 名古屋大学, Domestic conference
    Poster presentation

  • Genetic analysis of the domestication related traits using backcross derived lines in emmer wheat.
    Shimada Saori, Gyu Koichi, Vladutu Cristian, Ishii Takashige, Kianian Shahryar, MORI NAOKI
    Annual meeting of the Japanese Society of Plant Breeding, Mar. 2017, Japanese, 名古屋大学, ［目的] エンマーコムギ (Triticum turgidum ssp. dicoccum) は、コムギ属の中で最も初期に栽培化されて起源した栽培種の一つであり、初期農耕の起源と世界各地への伝播において重要な役割を果たした。本研究では、コムギ栽培化の初期に起こった遺伝的変化を解明することを目的として、祖先野生種 (T. turgidum ssp. dicoccoides) の遺伝的背景を持つ戻し交雑自殖系統を育成し、栽培化関連形質を評価することによりこれらを支配するQTLの解析を進めている。 ［方法］ 栽培エンマーコムギ (以下Dcm1001と呼ぶ)と野生エンマーコムギ (以下Dcc63) の交雑から野生種の遺伝的背景を持つ戻し交雑自殖系統 (BC2F14) を育成した（図１）。これらの栽培化関連形質を評価し、分子マーカーによるQTL解析により、, Domestic conference
    Poster presentation

  • インディカ型栽培イネIR36の非脱粒性を支配する新規遺伝子座の探索
    辻村 雄紀, 井上 千鶴, Than Myint Htun, 西村 彬則, 杉山 昇平, 岡 佑美, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第131回講演会, Mar. 2017, Japanese, 日本育種学会, 名古屋大学, Domestic conference
    Poster presentation

  • Genetic analysis of the domestication related traits using backcross derived lines in emmer wheat
    Shimada Saori, Gyu Koichi, Vladutu Cristian, Ishii Takashige, Kianian Shahryar, MORI NAOKI
    第11回ムギ類研究会, Dec. 2016, Japanese, 岡山大学 資源植物学研究所, エンマーコムギはコムギ属の中で最も初期に栽培化されたコムギの一つとされている。本研究では、エンマーコムギの栽培化の初期に起こった遺伝的変化の解明を目的として、野生エンマーコムギと栽培エンマーコムギから作出された野生種の遺伝的背景を持つ戻し交雑自殖系統について、それらの栽培化関連形質を調査した。また、分子マーカーによるQTL解析を行うことで、エンマーコムギの栽培化関連形質を支配するQTLの染色体上の位置や効果を推定した。, Domestic conference
    Poster presentation

  • 野生イネOryza rufipogon由来の穂の開帳性に関する遺伝子座と関連遺伝子の発現について
    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第130回講演会, Sep. 2016, Japanese, 日本育種学会, 鳥取大学, Domestic conference
    Poster presentation

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑自殖系統を用いた節からの出芽能力の評価
    池本 麻衣, 大八木 徹弥, Pham Thien Thanh, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第130回講演会, Sep. 2016, Japanese, 日本育種学会, 鳥取大学, Domestic conference
    Poster presentation

  • 野生イネOryza rufipogonとインディカ型栽培イネ（カサラス）の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索
    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, 日本育種学会, 鳥取大学, Domestic conference
    Oral presentation

  • ジャポニカ型栽培イネ（日本晴）の非脱粒性に関与した遺伝子座の推定
    辻村 雄紀, Than Myint Htun, 沼口 孝司, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, 日本育種学会, 鳥取大学, Domestic conference
    Poster presentation

  • ウメ遺伝資源における黒星病およびかいよう病抵抗性の評価
    沼口 孝司, 北村 祐人, 土田 靖久, 仲 慶晃, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第130回講演会, Sep. 2016, Japanese, 日本育種学会, 鳥取大学, Domestic conference
    Poster presentation

  • Oryza rufipogonとインディカ型栽培イネ（カサラス）の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索
    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, 日本育種学会, 鳥取大学, Domestic conference
    Oral presentation

  • Evaluation of seed-shattering behaviour in early rice domestication
    Ryo Ishikawa, Yumi Oka, Yuki Tsujimura, Natsumi Takama, Chizuru Inoue, Than Myint Htun, Takashige Ishii
    8th World Archaeology Congress, Sep. 2016, English, Doshisha University, International conference
    [Invited]
    Invited oral presentation

  • A closed panicle played an important role in rice domestication
    Ryo Nishioka, Koji Numaguchi, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii
    8th World Archaeology Congress, Sep. 2016, English, Doshisha University, International conference
    Poster presentation

  • 野生イネOryza rufipogon由来の穂の開帳性を支配する遺伝子座の推定と効果の検証
    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, ISHIKAWA RYO, ISHII TAKASHIGE
    イネ遺伝学・分子生物学ワークショップ, Jul. 2016, Japanese, 名古屋大学, Domestic conference
    Poster presentation

  • 熱帯アジアの野生イネOryza rufipogon
    ISHII TAKASHIGE
    イネ属近縁野生種研究会, Jul. 2016, Japanese, 遺伝学研究所, Domestic conference
    Oral presentation

  • インディカ型栽培イネ（カサラス）の非脱粒性を支配するsh4座以外の遺伝子座の探索
    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, 辻村 雄紀, Than Myint Htun, ISHII TAKASHIGE, ISHIKAWA RYO
    イネ遺伝学・分子生物学ワークショップ, Jul. 2016, Japanese, 名古屋大学, Domestic conference
    Poster presentation

  • 栽培イネの祖先となった野生イネとは？
    ISHII TAKASHIGE
    第６回北海道イネ研究会, Jun. 2016, Japanese, 北海道大学, Domestic conference
    Oral presentation

  • Identification of quantitative trait loci controlling seed-shattering behaviour in rice
    Yuki Tsujimura, Than Myint Htun, Chizuru Inoue, Takashige Ishii, Ryo Ishikawa
    PAG Asia 2016, Jun. 2016, English, Grand Copthorne Waterfront Hotel, International conference
    Poster presentation

  • Allelic interaction at awing loci in the genetic backgrounds of wild and cultivated rice
    Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii
    PAG Asia 2016, Jun. 2016, English, Grand Copthorne Waterfront Hotel, International conference
    Poster presentation

  • 野生イネと栽培イネの間で検出されたアルミニウムストレス耐性を支配する遺伝子座qAl1の遺伝学的解析
    和田 もりえ, 岩田 将英, 横正 健剛, 吉田 周作, Pham Tien Thanh, ISHII TAKASHIGE, 馬 建鋒, ISHIKAWA RYO
    日本育種学会第129回講演会, Mar. 2016, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Poster presentation

  • 野生イネおよび栽培イネの遺伝的背景における芒の長さを支配する遺伝子座の対立遺伝子間の相互作用
    池本 麻衣, 大塚 光晴, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第129回講演会, Mar. 2016, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Poster presentation

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑集団を用いた穂の開帳性に関するQTL解析
    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第129回講演会, Mar. 2016, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Poster presentation

  • オーストラリア由来の野生イネOryza meridionalisを用いた種子亜鉛濃度に関わる遺伝子座の同定
    岩田 将英, 谷古 健太, 門田 剛太郎, Orn Chhourn, 吉田 周作, ISHII TAKASHIGE, 馬 建鋒, ISHIKAWA RYO
    日本育種学会第129回講演会, Mar. 2016, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Poster presentation

  • インディカ型栽培イネKasalathの脱粒性喪失に関与した新規遺伝子座の探索
    岡 佑美, 井上 千鶴, Than Myint Htun, 竹中 祥尭, 辻村 雄紀, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第129回講演会, Mar. 2016, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Poster presentation

  • 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の解析
    Shimada Saori, Gyu Koichi, Vladutu Christian, Ishii Takashige, Kianian Shahryar, MORI NAOKI
    第10回 ムギ類研究会, Dec. 2015, Japanese, 三重大学農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連形質の解析を行った。その結果、まだメーカー数が藤生部であるが穂軸の脆弱性や１穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, Domestic conference
    Poster presentation

  • 栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連QTLの解析
    Miyazaki Yuki, Pham-Minh Ngoc, Vladutu Christian, Ishii Takashige, Kianian Shahryar, MORI NAOKI
    第10回 ムギ類研究会, Dec. 2015, Japanese, 三重大学 農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連QTLの解析を行った。その結果、穂軸の脆弱性や１穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, Domestic conference
    Poster presentation

  • Development of backcross inbred lines with genetic background of wild emmer wheat
    Mori Naoki, Gyu Koichi, Vladutu Cristian, Ishii Takashige, Kianian Shahryar
    日本育種学会第１２８回講演会, Sep. 2015, Japanese, 新潟大学, エンマーコムギ(Triticum turgidum ssp. dicoccum)は今から約1万年前に野生二粒系コムギ（別名パレスチナコムギ、T. turgidum ssp. dicoccoides）から栽培化されて誕生した。このコムギは新石器時代から数千年にわたってムギ農耕の主要な作物として利用されたことが知られている。我々はこの栽培化の過程でゲノムにどのような変化が起こったのかを明らかにするため、これまでに栽培種と野生種の交雑に由来するF2集団や栽培種の遺伝的背景に野生種の染色体断片を導入した戻し交雑系統群を用いて解析してきた。今回、栽培化初期の遺伝的変化を捉えることを目的として、野生二粒系コムギの遺伝的背景に栽培エンマーコムギの染色体断片を導入した戻し交雑系統群を作成したので報告する。, Domestic conference
    Poster presentation

  • 野生イネ(Oryza rufipogon) の遺伝的背景における種子脱粒性と穂の開帳性の関係
    井上 千鶴, Than Myint Htun, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第128回講演会, Sep. 2015, Japanese, 日本育種学会, 新潟大学, Domestic conference
    Poster presentation

  • Evaluation of the domestication-related traits in rice
    Ryo Ishikawa, Yumi Oka, Ryo Nishioka, Mai Ikemoto, Than Myint Htun, Chizuru Inoue, Koji Numaguchi, Takashige Ishii
    Early rice cultivation systems and their impact on social evolution and the environment., Sep. 2015, English, University College London, International conference
    [Invited]
    Invited oral presentation

  • Evaluation of genetic variation among wild and cultivated rice in Cambodia
    Orn Chhourn, Rieko Shishido, Masahiro Akimoto, Ryo Ishikawa, Than Myint Htun, Ryo Nishioka, Men Sarom, Sakhan Sophany, Ouk Makara, Takashige. Ishii
    日本育種学会第128回講演会, Sep. 2015, English, 日本育種学会, 新潟大学, Domestic conference
    Poster presentation

  • 野生イネOryza rufipogon由来の穂の開帳性を支配する遺伝子座の推定
    西岡 諒, 三日月 裕美, Than Myint Htun, ISHIKAWA RYO, ISHII TAKASHIGE
    近畿作物・育種研究会 第179回例会, May 2015, Japanese, 近畿作物・育種研究会, 奈良県立橿原考古学研究所, Domestic conference
    Oral presentation

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑自殖系統を用いた節からの出芽・出根能力およびひこばえの発生能力の評価
    池本 麻衣, 大八木 徹弥, Pham Thien Thanh, ISHIKAWA RYO, ISHII TAKASHIGE
    近畿作物・育種研究会 第179回例会, May 2015, Japanese, 近畿作物・育種研究会, 奈良県立橿原考古学研究所, Domestic conference
    Oral presentation

  • 野生イネと栽培イネのアルミニウムストレス耐性を支配する遺伝子座の同定とその効果
    和田 もりえ, 岩田 将英, 吉田 周作, Pham Thien Thanh, ISHII TAKASHIGE, 馬 建鋒, ISHIKAWA RYO
    日本育種学会第127回講演会, Mar. 2015, Japanese, 日本育種学会, 玉川大学, Domestic conference
    Poster presentation

  • インディカ型栽培イネ(Oryza sativa cv. IR36)における種子脱粒性の喪失に関与した遺伝子座数の推定
    西村 彬則, Than Myint Htun, 井上 千鶴, 岡 佑美, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第127回講演会, Mar. 2015, Japanese, 日本育種学会, 玉川大学, Domestic conference
    Poster presentation

  • Evaluation of genetic variation among wild rice populations in Cambodia
    Orn Chhourn, Rieko Shishido, Hiroki Kageyama, Masahiro Akimoto, Ryo Ishikawa, Makara Ouk, Sarom Men, Takashige Ishii
    日本育種学会第127回講演会, Mar. 2015, English, 日本育種学会, 玉川大学, Domestic conference
    Poster presentation

  • A closed panicle regulated by OsLG1 was a selected trait during rice domestication
    ISHII TAKASHIGE
    Plant & Animal Genome XXIII, Jan. 2015, English, San Diego, USA, International conference
    [Invited]
    Nominated symposium

  • Seed shattering behaviour and abscission layer formation in rice
    Cizuru Inoue, Than Myint Htun, Yumi Oka, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa
    Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, International conference
    Poster presentation

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座の相互作用と離層形態
    井上 千鶴, Than Myint Htun, INOUE KANAKO, IKEDA KENICHI, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第126回講演会, Sep. 2014, Japanese, 日本育種学会, 南九州大学, Domestic conference
    Poster presentation

  • Genetic analysis of domestication traits in emmer wheat using backcross inbred lines
    MORI NAOKI, Ngoc Pham Minh, Vladutu Cristian, Thanh Pham Thien, Thanh Phan Thi, Takashige Ishii, Kianian Shahryar
    Annual meeting of the Japanese Society of Plant Breeding, Sep. 2014, Japanese, 南九州大学, エンマーコムギ (Triticum turgidum ssp. dicoccum)は、約１万年前に野生種 (パレスチナコムギ、T. turgidum ssp. dicoccoides) から栽培化されて誕生した。このコムギは新石器時代から数千年にわたり西南アジアからヨーロッパにかけて主要な穀物として利用されたことが知られている。我々はコムギの栽培化初期の遺伝的変化を明らかにするため、エンマーコムギとパレスチナコムギのF1にエンマーコムギを戻し交雑し、エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を作成した。本講演では、この系統を用いて脱粒性などの栽培化関連形質のQTL解析を行ったので報告する。, Domestic conference
    Poster presentation

  • 野生イネと栽培イネのアルミニウムストレス耐性を支配する遺伝子座の同定
    和田 もりえ, 岩田 将英, 吉田 周作, Pham Thien Thanh, ISHII TAKASHIGE, 馬 建鋒, ISHIKAWA RYO
    イネ遺伝学・分子生物学ワークショップ2014, Jul. 2014, Japanese, 東京大学, Domestic conference
    Public symposium

  • 野生イネ(Oryza rufipogon)における種子脱粒性遺伝子座間の相互作用
    井上 千鶴, Than Myint Htun, Orn Chhourn, 岡 佑美, ISHII TAKASHIGE, ISHIKAWA RYO
    イネ遺伝学・分子生物学ワークショップ2014, Jul. 2014, Japanese, 東京大学, Domestic conference
    Public symposium

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用
    井上 千鶴, Than Myint Htun, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第125回講演会, Mar. 2014, Japanese, 日本育種学会, 東北大学, Domestic conference
    Oral presentation

  • 野生イネ(Oryza rufipogon)の遺伝的背景における栽培化関連形質の評価
    西村 彬則, 岩崎 友哉, 山本 知佳, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第125回講演会, Mar. 2014, Japanese, 日本育種学会, 東北大学, Domestic conference
    Oral presentation

  • Identification of a quantitative trait locus involved in abscission layer formation for seed shattering in Asian wild rice, Oryza rufipogon
    Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, Ryo Ishikawa
    日本育種学会第125回講演会, Mar. 2014, English, 日本育種学会, 東北大学, Domestic conference
    Oral presentation

  • Evaluation of genetic diversity among rice varieties in Cambodia
    Orn Chhourn, Ryo Ishikawa, Sakhan Sophany, Ouk Makara, Takashige Ishii
    7th International Conference of Rice Genetics, Nov. 2013, English, Dusit Thai Hotel Manila, Philippine, International conference
    Poster presentation

  • Closed panicles in rice domestication
    ISHII TAKASHIGE, Numaguchi K, Miura K, Yoshida K, Thanh PT, Htun TM, YAMASAKI MASANORI, Matsumoto T, Terauchi R, ISHIKAWA RYO, Ashikari M
    International Rice Genetics Symposium, Nov. 2013, English, Manila, Philippines, International conference
    Oral presentation

  • A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L.
    Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, Takashige Ishii
    7th International Rice Genetics Symposium, Nov. 2013, English, Dusit Thai Hotel Manila, Philippine, International conference
    Poster presentation

  • A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L.
    Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, Takashige Ishii
    7th International Conference of Rice Genetics, Nov. 2013, English, Dusit Thai Hotel Manila, Philippine, International conference
    Poster presentation

  • 穂が閉じることによって野生イネはどう変化したか？
    沼口 孝司, Htun Than Myint, Thanh Pham Thien, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第124回講演会, Oct. 2013, Japanese, 日本育種学会, 鹿児島大学, Domestic conference
    Oral presentation

  • Evaluation of genetic variation among wild rice populations of Oryza rufipogon
    ISHII TAKASHIGE
    Expert Consultation Workshop on the Use of Rice Wild Relatives for Rice Improvement, Oct. 2013, English, Shizuoka, Japan, International conference
    [Invited]
    Invited oral presentation

  • Genetic analysis of domestication traits in emmer wheat using an F2 population
    Naoki Mori, Phan Thi Thanh, Cristian Vladutu, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Shahryar Kianian
    12th International Wheat Genetics Symposium,, Sep. 2013, English, Pacifico Yokohama, Japan, Emmer wheat (Triticum turgidum ssp. dicoccum) was a principal crop in the development and spread of Neolithic agriculture in the Old World. It represents the primitive situation in the domestication of AABB tetraploid wheat. It has non free-threshing (hulled) habit and relatively fragile rachis, although it has non-shattering spike. The more advanced forms of tetraploid whea, International conference
    Poster presentation

  • Molecular genetic analysis of the QTLs involved in the domestication of emmer wheat
    Thanh P. T, C. Vladutu, S. Kianian, T. Ishii, T. Pham, S. Nasuda, M. Nitta, N. Mori
    日本育種学会 第122回講演会, Sep. 2012, English, 京都産業大学, Significant association between wheat and human began in southwest Asia more than 10000 years ago. Emmer wheat was one of the founder crop that was domesticated from wild emmer wheat. In the present study, QTL analysis was carried out to estimate the valuable QTLs underlying the domestication of emmer wheat., Domestic conference
    Oral presentation

  • Evaluation of genetic variation among wild populations and local varieties of rice in Vietnam
    ISHII TAKASHIGE
    Rice Symposium: Trends in Rice Research to Overcome Stresses in a context of Climate Change, Sep. 2012, English, Hanoi, Vietnam, International conference
    [Invited]
    Invited oral presentation

  • Evaluation of genetic variation among wild populations and local varieties in rice
    ISHII TAKASHIGE
    Rice and Language across Asia Symposium, Nov. 2011, English, Cornell Univ, USA, International conference
    [Invited]
    Invited oral presentation

  • 山田錦の両親系統における酒造好適性にかかわる染色体領域の推定
    Hachikenn Takehiro, Yoshida Shinya, Ishii Takashige, Ohta Jyun, Akashi Takahiro, Nishimura Akira, MORI NAOKI
    日本育種学会 第120回講演会, Sep. 2011, Japanese, 福井県立大学、福井, Domestic conference
    Poster presentation

  • 野生イネの遺伝的背景における栽培イネの非脱粒性遺伝子の効果
    ISHIKAWA RYO, Than Myint Htun, YAMASAKI MASANORI, Pham Thien Thanh, ISHII TAKASHIGE
    第52回日本植物生理学会年会, Mar. 2011, Japanese, 日本植物生理学会, 東北大学, Domestic conference
    Oral presentation

  • 野生イネ (Oryza rufipogon)の種子有芒性の評価および遺伝子マッピング
    大塚 光晴, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第119回講演会, Mar. 2011, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Oral presentation

  • 栽培イネ Oryza sativa Nipponbareを野生イネO. rufipogon W630で戻し交雑した自殖系統群を用いた植物と種子の形態形質に関するQTL解析
    山本 知佳, 岩崎 友哉, ISHIKAWA RYO, ISHII TAKASHIGE
    日本育種学会第119回講演会, Mar. 2011, Japanese, 日本育種学会, 横浜市立大学, Domestic conference
    Oral presentation

  • Wild rice: Interesting materials for plant breeding and genetics
    ISHII TAKASHIGE
    Special Seminar in International Platform for Asian Agricultural Education, Nov. 2010, English, Kasetsart Univ, Thailand, International conference
    Public discourse

  • Domestication of rice
    ISHII TAKASHIGE
    Special Seminar in International Platform for Asian Agricultural Education, Nov. 2010, English, Chiang Mai Univ, Thailand, International conference
    Public discourse

  • Gene mapping using molecular markers
    ISHII TAKASHIGE
    International Workshop on Agrobioresource Conservation and Pest Management in Southeast Asia, Jun. 2010, English, Kobe, Japan, International conference
    Public discourse

  • QTL analysis for spikelet opening time using backcross inbred lines between Oryza sativa Nipponbare and O. rufipogon
    Pham Thien Thanh, Phan Dang, Thai Phuong, Ryo Ishikawa, Takashige Ishii
    日本育種学会第117回講演会, Mar. 2010, English, 日本育種学会, 京都大学, Domestic conference
    Oral presentation

  • Estimation of outcrossing rate of Asian wild rice, Oryza rufipogon, under field condition
    Phan Dang Thai Phuong, Hiroki Kageyama, Ryo Ishikawa, Takashige Ishii
    日本育種学会第117回講演会, Mar. 2010, English, 日本育種学会, 京都大学, Domestic conference
    Oral presentation

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare
    Pham Thien Thanh, Ryo Ishikawa, Naoki Mori, Takashige Ishii
    6th International Conference of Rice Genetics, Nov. 2009, English, Manila Hotel, Philippine, International conference
    Poster presentation

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare
    Thanh PT, ISHIKAWA RYO, MORI NAOKI, ISHII TAKASHIGE
    International Rice Genetics Symposium, Nov. 2009, English, Manila, Philippines, International conference
    Poster presentation

  • Evaluation of genetic diversity among wild rice populations of Oryza rufipogon in Myanmar and Cambodia
    ISHII TAKASHIGE
    International Symposium on Wild Rice, Nov. 2009, English, Bangkok, Thailand, International conference
    [Invited]
    Invited oral presentation

  • Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species
    MORI NAOKI, Hidehira, Y, Ishii, T, Nakamura, C
    4th International Conference on Energy Efficiency and Agricultural Engineering, Oct. 2009, English, Rousse, Bulgaria, International conference
    Oral presentation

  • Intraspecific variation of chloroplast DNA in Aegilops speltoides
    Naoki Mori, Hiroyuki Watatani, Takashige Ishii, Yasutaka Kondo, Taihachi Kawahara, Chiharu Nakamura
    6th International Triticeae Symposium, Jun. 2009, English, Kyoto University, Kyoto, Japan, International conference
    Poster presentation

  • Evaluation of genetic variation in rice
    ISHII TAKASHIGE
    Asian International Symposium for Agriculture, Mar. 2009, English, Kobe, Japan, International conference
    Oral presentation

  • Mitochondrial microsatellite variability in the ancestral species of wheat (Aegilops speltoides Tausch.)
    Yoshiaki Ohmichi, Takashige Ishii, Chiharu Nakamura, Naoki Mori
    GENES & GENETIC SYSTEMS, Dec. 2007, English, GENETICS SOC JAPAN

  • 野生イネ由来の種子脱粒性、穂の開帳性、種子色を支配する遺伝子のマッピング
    ISHII TAKASHIGE, 崎山 弘樹, ISHIKAWA RYO, KAMIJIMA OSAMU
    近畿作物育種研究会, Jul. 2005, Japanese, 近畿作物育種研究会, 神戸大学, Domestic conference
    Oral presentation

  • 野生イネ由来の種子脱粒性, 穂の開帳性, 種子色を支配する遺伝子のマッピング
    石井 尊生, 崎山 弘樹, 石川 亮, 上島 脩志
    近畿作物・育種研究会第159回例会, 2005, Japanese, 日本育種学会, 神戸大学, Domestic conference
    Oral presentation

  • 野生イネ遺伝資源のオンファーム保存システム構築のための実証的調査Ⅰ ミャンマーにおける事例
    秋本 正博, 宍戸 理恵子, 石井 尊生, 石川 隆二, 上埜 喜八, 中村 郁郎, 佐藤 洋一郎, 佐藤 雅志
    日本育種学会（育種学研究第７卷別冊1-2号p.296）, 2005, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • 野生イネOryza rufipogon由来の光合成能力関連QTLをもつ準同質遺伝子系統の光合成特性
    増本 千都, 石井 尊生, 池田 延行, 畠中 知子, 内田 直次
    日本作物学会（日本作物学会紀事74巻第1号96-97）, 2005, Japanese, 日本作物学会, 未記入, Domestic conference
    Poster presentation

  • 普通糸コムギならびにその祖先種でみられたコムギミトコンドリアマイクロサテライト座における多様性
    高橋 知佐, 森 直樹, 上島 脩志, 石井 尊生
    近畿作物・育種研究会第159回例会, 2005, Japanese, 日本育種学会, 神戸大学, Domestic conference
    Oral presentation

  • 耐塩性および非耐塩性イネ間のFｌ雑種の葯培養における培地中NaCl濃度の影響
    三十尾 修司, 松本 学, Javed, M. A, 石井 尊生, 上島 脩志
    日本育種学会（育種学研究第７卷別冊1-2号p.236）, 2005, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • ミャンマーにおける野生イネの調査
    石井 尊生
    日本育種学会（育種学研究第７卷別冊1-2号p.80）, 2005, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • The salinity tolerance evaluation of two eco-types of Oryza sativa L. at different growth stages in successive salinity levels.
    Javed M. A, ISHII Takashige, KAMIJIMA Osamu, MISOO Shuji
    3rd Int. Conf. on Sustainable Agriculture for Food, Energy, and Industry,, 2005, English, 未記入, Canada, International conference
    Oral presentation

  • QTL analysis for several agronomic characters using BC2 populations between Oryza meridionalis and O. sativa.
    ISHII Takashige, ITO K, HARA D, KOBAYASHI N, UCHIDA Naotsugu, KAMIJIMA Osamu
    5th Int. Rice Genet. Symp,, 2005, English, 未記入, Philippines, International conference
    Oral presentation

  • Production and evaluation of chromosome segment substitution lines having wild QTL regions for yield, photosynthesis activity and grain width between Oryza sativa cv. IR36 and O. rufipogon.
    IKEDA N, ASHIDA T, MASUMOTO C, UCHIDA Naotsugu, KAMIJIMA Osamu, ISHII Takashige
    5th Int. Rice Genet. Symp,, 2005, English, 未記入, Philippines, International conference
    Oral presentation

  • Improvement of the anther culture efficiency in recalcitrant salt tolerant indica rice varieties.
    Javed M. A, ISHII Takashige, KAMIJIMA Osamu, MIOO Shuji
    The 1st. Int.. Symp. on Sustainable Agriculture for Subtropical Regions, 67,, 2005, English, 未記入, China, International conference
    Oral presentation

  • 栽培イネ(Oryza sativa Nipponbare)の遺伝背景における野生イネ(Oryza rufipogon)由来の脱粒性遺伝子の作用について
    二村 直人, ISHIKAWA RYO, KAMIJIMA OSAMU, ISHII TAKASHIGE
    日本育種学会第106回講演会, Sep. 2004, Japanese, 日本育種学会, 三重大学, Domestic conference
    Oral presentation

  • イネ品種「兵庫北錦」と「北陸142号」の雑種集団を用いた心白発現及び粒大に関するQTL分析
    澤田 桂子, 久世 淳子, 吉田 晋也, 池上 勝, 中村 千春, 石井 尊生, 上島 脩志
    日本育種学会第106回講演会, Sep. 2004, Japanese, 日本育種学会, 三重大学, Domestic conference
    Oral presentation

  • 野生イネOryza. rufipogon および栽培イネO. sativa Nipponbare交配由来のBC2F4集団を用いた諸形質に関するQTL解析
    山下 晃弘, 内田 直次, 上島 脩志, 石井 尊生
    日本育種学会, 2004, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 栽培イネ（Oryza sativa Nipponbare) の遺伝的背景における野生イネ（O. rufipogon)由来の脱粒性遺伝子の作用について
    二村 直人, 石川 亮, 上島 脩志, 石井 尊生
    日本育種学会, 2004, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • イネ品種「兵庫北錦」と「北陸142号」の雑種集団を用いた心白発現および粒大に関するQTL解析
    澤田 桂子, 久世 淳子, 吉田 晋弥, 池上 勝, 中村 千春, 石井 尊生, 上島 脩志
    日本育種学会, 2004, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • Estimation and comparison of salinity tolerance during seedling stage in several indica rice cultivars.
    JAVED M A, MISOO Shuji, ISHII Takashige, KAMIJIMA Osamu
    日本育種学会, 2004, English, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 野生イネOryza rufipogon由来の脱粒性遺伝子のマッピング
    二村 直人, ISHIKAWA RYO, KAMIJIMA OSAMU, ISHII TAKASHIGE
    日本育種学会第104回講演会, Sep. 2003, Japanese, 日本育種学会, 神戸大学, Domestic conference
    Oral presentation

  • 祖先２倍種の葉緑体DNA における分子多型から見た４倍性コムギの起原
    綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹
    日本育種学会 育種学研究, Sep. 2003, Japanese, 日本育種学会, 神戸大学, Domestic conference
    Oral presentation

  • 野生イネ（Oryza rufipogon）の二つのアルコール脱水素酵素遺伝子座領域（Adh1 とAdh2）における塩基多型
    吉田 健太郎, 石井 尊生, 宮下 直彦
    日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 野生イネでみられた葉緑体およびミトコンドリアマイクロサテライト座における多型性
    隈本 寿彦, 佐藤 雅志, 石川 隆二, 佐藤 洋一郎, 上島 脩志, 石井 尊生
    日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 野生イネOryza rufipogon 由来の脱粒性遺伝子のマッピング
    二村 直人, 石川 亮, 上島 脩志, 石井 尊生
    日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 米アミロペクチンの超長鎖合成の遺伝解析
    青木 法明, 梅本 貴之, 石井 尊生, 吉田 晋弥, 上島 脩志, 松倉 潮, 井ノ内 直良
    日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 窒素固定バクテリアエンドファイトHerbaspirillum sp.B501 の内生菌数にみられる野生および栽培イネ間差異
    安部 匡, 南沢 究, 石井 尊生, 東谷 篤志, 佐藤 雅志
    神戸大学農学部秋季公開セミナー－農学を新発見－．, 2003, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • 祖先２倍種の葉緑体DNA における分子多型からみた４倍性コムギの起原
    綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹
    日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • 栽培イネOryza sativa × 野生イネO. rufipogon のBC2F2 系統の光合成特性− Rubisco, Rubiscoactivase， スクロース代謝関連酵素に関して−
    増本 千都, 石井 尊生, 内田 直次
    日作紀, 2003, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • イネ栽培種×野生種交雑系統BC2F4 系統の光合成と生長
    増本 千都, 石井 尊生, 内田 直次
    熱帯農業, 2003, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • アズキにおけるマイクロサテライトマーカーの開発
    松尾 明子, 渡辺 純平, 池田 優子, 伊勢村 武久, 石井 尊生, 上島 脩志
    日本育種学会，育種学研究, 2003, Japanese, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • QTL analysis for several agronomic characters using four BC2 poppulations between wild and cultivated rice species.
    ISHII Takashige
    Int. Genet. Resour. Workshop on the genus Oryza., 2003, English, 未記入, 未記入, Domestic conference
    Oral presentation

  • Effects of culture media and alternate temperatures on anther culture of salt tolerant indica rice cultivars.
    JAVED M A, MISOO Shuji, ISHII Takashige, KAMIJIMA Osamu
    日本育種学会，育種学研究, 2003, English, 日本育種学会, 未記入, Domestic conference
    Oral presentation

  • イネ野生種（Oryza rufipogon）由来の脱粒性遺伝子のマッピング
    ISHIKAWA RYO, KAMIJIMA OSAMU, ISHII TAKASHIGE
    日本育種学会第101回講演会, Mar. 2002, Japanese, 日本育種学会, 玉川大学, Domestic conference
    Oral presentation

  • Construction of a linkage map using interspecific population of Vigna umbellata*V. angularis.
    加賀秋人, 月本恭幸, 所英理子, 石井尊生, 木村忠彦, 上島おさ志
    育種学雑誌, 1996, Japanese

  • Genetic analysis of morphological characters and RAPD markers in F2 population of Vigna angularisx V. nakashimae.
    加賀秋人, 大西昌子, 石井尊生, 三十尾修司, 木村忠彦, 上島おさ志
    育種学雑誌, 1994, Japanese

■ Affiliated Academic Society
  • 近畿作物育種研究会

  • 日本遺伝学会

  • 日本育種学会

■ Research Themes
  • -
    石井 尊生, 齋藤 大樹, 佐藤 豊, 石川 亮
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Kobe University, Apr. 2022 - Mar. 2026, Principal investigator

  • 石井 尊生
    日本学術振興会, 科学研究費, 基盤研究(B), Apr. 2018 - Mar. 2022, Principal investigator
    Competitive research funding

  • 石井 尊生
    日本学術振興会, 科学研究費, 挑戦的研究（萌芽）, Jun. 2018 - Mar. 2021, Principal investigator
    Competitive research funding

  • 石井 尊生
    日本学術振興会, 科学研究費, 特別研究員奨励費, Apr. 2016 - Mar. 2018, Principal investigator
    Competitive research funding

  • 石井 尊生
    日本学術振興会, 科学研究費, 基盤研究(B), Apr. 2014 - Mar. 2018, Principal investigator
    Competitive research funding

  • 石井 尊生, 宍戸 理恵子, 石川 亮, 野々村 賢一, 小出 陽平
    日本学術振興会, 科学研究費, 基盤研究(B), Apr. 2014 - Mar. 2017, Principal investigator
    Competitive research funding

  • ISHII TAKASHIGE
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2011 - Mar. 2014, Principal investigator
    In order to investigate important loci for rice domestication, QTL analysis of domestication-related traits was carried out with four backcross recombinant inbred populations derived from the reciprocal crosses between two rice cultivars (Japonica and Indica types) and an Asian wild rice accession. The results suggest that QTLs, commonly detected in the two populations having cultivated or wild genetic backgrounds, may have played important roles in the early phase of rice domestication.
    Competitive research funding

  • 石井 尊生
    日本学術振興会, 科学研究費, 特別研究員奨励費, 神戸大学, 2011 - 2013, Principal investigator
    本研究は、アジアの野生イネ(Oryza rufipogon W630)を2種類の典型的な栽培品種(O. sativa Japonica NipPonbareおよびO. sativa Indica IR36)で戻し交雑した自殖系統を用いて、野生イネ由来の有用農業形質に関する新規遺伝子を探索することを目的としている。 まず、O. sativa Japonica Nipponbareで戻し交雑した161の自殖系統については、昨年度第4および第5染色体上の2カ所に早朝開花性に関するQTLが検出された。そこで、それぞれの領域で様々な長さの染色体断片を持つ個体を選抜し形質調査を行ったところ、1カ所のQTL領域のみを持つ個体では有意な早朝開花性は概察されなかった。一方、第4および第5染色体上の2カ所のQTL領域を持っ個体では早朝開花性がみられた。そのため、これら2つのQTL領域を野生イネから栽培イネに導入すれば、早朝開花性によって高温を回避できる優良栽培品種が育成できると考えられた。 もう1つの、O. sativa Indica IR36で戻し交雑した172系統からなる戻し交雑自殖系統については、5っの種子形質(種子重、種子長、種子幅、玄米長、玄米幅)および5つの農業形質(出穂期、桿長、穂長、穂形、分げつ数)に関するQTL解析を行った。その結果、合計15カ所のQTLが推定された。昨年度、同じ形質に関してNipponbare遺伝的背景の系統で検出されたものと比較したところ、第1染色体上の稗長、第4染色体上の穂形ならびに第8染色体上の出穂期に関するQTL領域が重複していた。これらのうち、第1および第4染色体の領域にはSD1ならびにSPR3の遺伝子座が含まれていたため、これらはイネ栽培種JapollicaおよびIndicaの双方の遺伝的背景で効果を持っ遺伝子座であると考えられた。
    Competitive research funding

  • ISHII Takashige
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2008 - Mar. 2011, Principal investigator
    In order to clarify key genes for rice domestication, the agronomical effects of non-spreading panicles of cultivated rice were analyzed. Near-isogenic lines for non-spreading panicles in the genetic background of wild rice were subjected to the field experiments. As a result, plants with closed panicles showed significantly reduced seed-shattering and outcrossing rates. These results suggests that a simple morphological change in panicle shape had a large impact on seed-shattering and pollinating behaviors and a strong influence on rice domestication.
    Competitive research funding

  • ISHII Takashige, SHISHIDO Rieko, AKIMOTO Masahiro, ISHIKAWA Ryuji, SATO Yoichiro, SATO Tadashi, NAKAMURA Ikuo, DR. Nguyen Thi Lang, MR. Nguyen Nien Chau, DR. Men Saron, DR. Tin Htut, MR. Than Sein, MR. Minn San Thein
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, Apr. 2006 - Mar. 2009, Principal investigator
    熱帯アジアのミャンマー(イラワジデルタ周辺および西部地帯)、ベトナム(メコンデルタ周辺)およびカンボジア(プノンペンおよびトンレサップ湖周辺)にて、栽培イネの祖先となった野生イネの遺伝資源としての遺伝学的および生態学的特性を明らかにするため、野生イネ自生集団における遺伝的多様性をDNA レベルで調査するとともに、それらと生態型や集団を取り巻く環境などの様々な要因との関連性の評価を行った。
    Competitive research funding

  • 石井 尊生
    日本学術振興会, 日仏交流促進事業（SAKURAプログラム）, 神戸大学, Sep. 2006 - Aug. 2008, Principal investigator, Co-authored internationally
    Competitive research funding

  • イネ近縁野生種由来の収量関連形質の遺伝解析
    石井 尊生
    農林水産省, 受託研究：有用遺伝子活用のための植物（イネ）・動物ゲノム研究, 神戸大学, Apr. 2002 - Mar. 2007, Principal investigator

  • 野生イネに潜む有用形質を支配する遺伝子の検出―野生種を利用した稲育種に向けて―
    石井 尊生
    日本学術振興会, 科学研究費助成事業, 奨励研究(A), 神戸大学, 2000 - 2001
    イネ野生種が持つ栽培種に存在しない有用遺伝子を検出するため、以下の実験を行った。まず、野生イネの材料としてミヤンマー由来のOryza rufipogon W630を用い、2種類の典型的な栽培品種(O. sativa Japonica cv. NipponbareおよびO. sativa Indica cv. IR36)で交雑し、それぞれ約200個体からなるBC_2戻し交雑集団を作成した。次に、これらを圃場に展開し、出穂期、最大光合成能、桿長、穂長、有効分げつ数、、種子100粒重、籾長、籾幅、玄米長、玄米幅、収量の11形質について調査した。BC_2集団は様々な形質を調査し終わる登熟期まで、葉を大量にサンプリングできなかったので、各BC_2自植後代約15系統の幼葉をまとめて回収しDNAを抽出した。これらのDNAは鋳型として調整し、マイクロサテライトマーカー座についての遺伝子型を調べるためにPCRを行った。なお、使用したマイクロサテライトマーカーは交配親間で多型がみられ、ほぼゲノムをカバーしている約75マーカーであった。11形質についてのデータならびに分子マーカー座の遺伝子型のデータをもとに、量的形質遺伝子座(QTL)解析を行ったところ、有意となった領域はNipponbare戻し交雑集団では55ヶ所、IR36戻し交雑集団では51ヶ所見出された。これらのうち、それぞれ半数強の領域において、野生種の対立遺伝子が各形質の計測値を増加させる効果を持っていた。2つの異なった栽培品種の遺伝的背景においては、野生種由来のQTLは6領域で共通していた。収量に関しては、第1染色体上の1領域で検出された野生種のQTLが両集団において収量を向上させる効果を持っていた。従って、この野生種由来のQTLは栽培イネのJaponicaおよびlndica双方において収量を向上させる効果を持つ可能性が示唆された。

  • 種およびゲノム特異的突然変異に基づくイネ属植物の系統進化の解明
    石井 尊生
    日本学術振興会, 科学研究費助成事業, 奨励研究(A), 神戸大学, 1997 - 1998, Principal investigator
    分子レベルでの変異に基づくイネ属植物の系統進化の解明のため、以下の実験を行った。材料はAゲノム種より栽培種2種29系統、野生種2種30系統、Aゲノム以外の種より野生種6種20系統(Cゲノム種3系統、CDゲノム種5系統、BCゲノム種5系統、Eゲノム種4系統、Fゲノム種1系統、未同定4倍体ゲノム種1系統)の計79系統を用いた。これらよりDNAを抽出し、まず葉緑体ゲノムにおける遺伝子間領域20カ所をPCR法により増幅した。Aゲノム種内では増幅断片長にほとんど変異がみられなかったが、イネ属植物内では約半数の領域で欠失により生じた多型がみられた。次に、核・葉緑体の両ゲノムの単純反復配列領域についてもPCR法により増幅を行った。核ゲノム由来の24領域と葉緑体ゲノム由来の10領域において検出された増幅断片長の多型の割合を比較したところ、核ゲノムにおける単純反復配列領域は非常に増幅突然変異に富むことが観察された。以上の結果より、イネ属植物における系統関係について以下のことが明らかになった。イネ属植物内では、E、Fゲノム種が他のものと比較して大きく分化しており、A、BC、C、CDゲノム種はそれぞれ同程度に分化しクラスターを形成している。Aゲノム種内においては、野生種のO.perennisのオセアニア、アフリカおよびアメリカ型が大きく分化しており、栽培種のO.sativaはO.perennisのアジア型と、もう1つの栽培種であるO.glaberrimaは野生種のO.breviligulataとグループを作ることが明らかになった。これは、栽培種のO.sativaとO.glaberrimaの祖先野生種はそれぞれO.perennisのアジア型とO.breviligulataであることを示唆するものであった。

  • RFLPおよびRAPD分析法による栽培イネの祖先の解明
    石井 尊生
    日本学術振興会, 科学研究費助成事業, 奨励研究(A), 神戸大学, 1995 - 1995, Principal investigator
    本研究では分子生物的手法を用いて栽培イネの祖先を明らかにすることを試みた。材料は栽培イネを含むAゲノム種より、栽培種2種14系統(O. sativa8系統、O. glaberrima6系統)、栽培イネと同じゲノム構成を持つ野生種2種16系統(O. perennis13系統、O. breviligulata3系統)の、計30系統を用いた、これらよりDNAを抽出し、分子レベルでの変異を検出するため、核、葉緑体、ミトコンドリアの3つのゲノムについてのRFLP分析および全DNAを用いたRAPD分析を行った。さらに、これら4つの分析それぞれについて、各系統間における類緑度を求め系統樹を作成した。まず、核、葉緑体、ミトコンドリアの3つのゲノム間における分化の違いについては、これらはほぼ同調して分化していたが、特に核ゲノムでの分化の程度が他の2つより大きいことが明らかになった。さらに、RFLP分析とRAPD分析の変異の検出頻度について比較したところ、RAPD分析の方がより多くの変異を検出できることがわかった。以上の結果をまとめ、総合的に分子レベルにおける栽培イネと野生イネとの系統関係について調べたところ、Aゲノム種内において、野生種のO. perennisのオセアニア、アフリカおよびアメリカ型が大きく分化しており、栽培種のO. sativaはO. perennisのアジア型と、もう1つの栽培種であるO. glaberrimaは野生種のO. brebiligulataとグループを作ることが明らかになった。これは、栽培種のO. sativaとO. glaberrimaの祖先野生種はそれぞれO. perennisのアジア型とO. breviligulataであることを示唆するものであった。

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