Research activity information

• Jul. 2010 日本先天異常学会, 第50回日本先天異常学会学術集会 優秀ポスター賞, Sry 導入XXマウスにおける雄性生殖腺の形態学的解析

  YOKOYAMA TOSHIFUMI

  Japan society

• Effect of the neonicotinoid pesticide clothianidin at a no-observed-adverse-effect-level (NOAEL) dose on maternal behavior in pregnant mice and their female offspring

  Sarika NUNOBIKI, Ayano YOSHIMOTO, Makiko ITO, Sakura YONOICHI, Yukako HARA, Yuya ISHIDA, Rinako MORISHITA, Youhei MANTANI, Toshifumi YOKOYAMA, Tetsushi HIRANO, Yoshinori IKENAKA, Nobuhiko HOSHI

  Japanese Society of Veterinary Science, Apr. 2025, Journal of Veterinary Medical Science, 87(4) (4), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Histological study on the postnatal development of the nerve network in the rat ileal mucosa and submucosa.

  Rinako Morishita, Satoki Nakanishi, Toshifumi Yokoyama, Nobuhiko Hoshi, Youhei Mantani

  We have previously reported detailed structures of the mucosal nerve network in the rat ileum, but the mechanisms underlying the development of this nerve network remain unclear. Therefore, we aimed to clarify the developmental process of the mucosal nerve network and submucosal neurons (SM-neurons) or ganglia (SMG), which are the main source of nerve fibers projected to the mucosa, in the rat ileum during the postnatal period. Immunohistochemistry against tubulin beta III (Tuj1) revealed that Tuj1-immunopositivities were more abundant in the lamina propria at 2 weeks old (2wk; pre-weaning) than at postnatal day 0 (P0) or 4 weeks old (4wk; post-weaning) and more frequent on the mesenteric side than on the antimesenteric side at 2wk. Hu antigen D (HuD)-immunopositive SM-neurons and SMG were also more abundantly localized on the mesenteric side than the antimesenteric side at P0 and 2wk. On the other hand, cells immunopositive for SRY-related HMG-box 10 (Sox10), which is the marker for enteric nervous system progenitor cells and enteric glial cells, were homogenously scattered in the submucosa throughout the entire circumference at all ages. Glial cell marker S100 calcium-binding protein B (S100β) in the submucosa was detected at all ages without any significant difference between the mesenteric and antimesenteric sides. These findings indicate that SMG formation and associated neurite extension into the mucosa in the rat ileum might occur preferentially on the mesenteric side by the weaning period, leading us to hypothesize that the mechanism by which the mucosal nerve network and SMG develop differs along the mesenteric-antimesenteric side axis.

  Feb. 2025, Cell and tissue research, 400, 71 - 80, English, International magazine

  [Refereed]

  Scientific journal


• Effects of low-dose subchronic exposure to the phenylpyrazole insecticide fipronil in juvenile mice

  Ayano YOSHIMOTO, Sarika NUNOBIKI, Makiko ITO, Sakura YONOICHI, Yukako HARA, Yuya ISHIDA, Youhei MANTANI, Toshifumi YOKOYAMA, Tetsushi HIRANO, Yoshinori IKENAKA, Nobuhiko HOSHI

  Corresponding, Japanese Society of Veterinary Science, 2025, Journal of Veterinary Medical Science, 87(4) (4), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine.

  Shota Murase, Youhei Mantani, Nobuhiko Ohno, Asaka Shimada, Satoki Nakanishi, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi

  We previously clarified the histological characteristics of macrophages in the rat small intestine using serial block-face scanning electron microscopy (SBF-SEM). However, the regional differences in the characteristics of macrophages throughout the large intestine remain unknown. Here, we performed a pilot study to explore the regional differences in the ultrastructure of mucosal macrophages in the large intestine by using SBF-SEM analysis. SBF-SEM analysis conducted on the luminal side of the cecum and descending colon revealed macrophages as amorphous cells possessing abundant lysosomes and vacuoles. Macrophages in the cecum exhibited a higher abundance of lysosomes and a lower abundance of vacuoles than those in the descending colon. Macrophages with many intraepithelial cellular processes were observed beneath the intestinal superficial epithelium in the descending colon. Moreover, macrophages in contact with nerve fibers were more prevalent in the cecum than in the descending colon, and a subset of them surrounded a nerve bundle only in the cecum. In conclusion, the present pilot study suggested that the quantity of some organelles (lysosomes and vacuoles) in macrophages differed between the cecum and the descending colon and that there were some region-specific subsets of macrophages like nerve-associated macrophages in the cecum.

  Mar. 2024, Cell and tissue research, English, International magazine

  [Refereed]

  Scientific journal


• Quantification of the tissue distribution and accumulation of the neonicotinoid pesticide clothianidin and its metabolites in maternal and fetal mice.

  Tetsushi Hirano, Shuji Ohno, Yoshinori Ikenaka, Kanoko Onaru, Shizuka Kubo, Yuka Miyata, Mizuki Maeda, Youhei Mantani, Toshifumi Yokoyama, Collins Nimako, Yared Beyene Yohannes, Shouta M M Nakayama, Mayumi Ishizuka, Nobuhiko Hoshi

  Neonicotinoids (NNs) are commonly used pesticides that have a selective agonistic action on insect nicotinic acetylcholine receptors. Recent evidence has shown that NNs have adverse effects in the next generation of mammals, but it remains unclear how NNs transferred from dams to fetuses are distributed and accumulated in fetal tissues. Here, we aimed to clarify the tissue distribution and accumulation properties of the NN clothianidin (CLO) and its 6 metabolites in 7 tissues and blood in both dams and fetuses of mice administered CLO for a single day or for 9 consecutive days. The results showed that the total concentrations of CLO-related compounds in the brain and kidney were higher in fetuses than in dams, whereas in the liver, heart, and blood they were lower in fetuses. The multi-day administration increased the total levels in heart and blood only in the fetuses of the single administration group. In addition, dimethyl metabolites of CLO showed fetus/dam ratios >1 in some tissues, suggesting that fetuses have higher accumulation property and are thus at higher risks of exposure to CLO-related compounds than dams. These findings revealed differences in the tissue-specific distribution patterns of CLO and its metabolites between dams and fetuses, providing new insights into the assessment of the developmental toxicity of NNs.

  Feb. 2024, Toxicology and applied pharmacology, 116847 - 116847, English, International magazine

  [Refereed]

  Scientific journal


• No-observed-adverse-effect-level (NOAEL) clothianidin, a neonicotinoid pesticide, impairs hippocampal memory and motor learning associated with alteration of gene expression in cerebellum.

  Yukako Hara, Asuka Shoda, Sakura Yonoichi, Yuya Ishida, Midori Murata, Mako Kimura, Makiko Ito, Sarika Nunobiki, Ayano Yoshimoto, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Yoshinori Ikenaka, Yoshiaki Tabuchi, Nobuhiko Hoshi

  Neonicotinoid pesticides (NNs) have been associated with numerous neurobehavioral effects in rodents, raising concerns about their impact on cognitive function. Clothianidin (CLO), a type of NN, was orally administered to male mice (10 weeks old, C57BL/6N) at the no-observed-adverse-effect level (NOAEL) of 50 mg/kg/day as indicated in the pesticide risk assessment report. Behavioral tests (novel location recognition and rotarod tests) evaluated hippocampal memory and cerebellar motor learning. After each test, plasma monoamines (3-methoxytyramine, histamine, serotonin, tryptamine) were measured by LC-ESI/MS/MS (Liquid chromatography-electrospray ionization/tandem mass spectrometry), and cerebellar mRNA expression was quantified by microarray and qRT-PCR analyses. The NOAEL of CLO was found to impair hippocampal memory, leading to decreased spontaneous locomotor activity and motor function. We reported, for the first time, multiple alterations of gene expression in the cerebellum associated with motor dysfunction.

  Feb. 2024, The Journal of veterinary medical science, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) in a mouse model of atopic dermatitis.

  Yuya Ishida, Sakura Yonoichi, Yukako Hara, Asuka Shoda, Mako Kimura, Midori Murata, Makiko Ito, Sarika Nunobiki, Ayano Yoshimoto, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Eiko Matsuo, Yoshinori Ikenaka, Nobuhiko Hoshi

  The effects of exposure to clothianidin (CLO), a neonicotinoid pesticide (NN), on the thymus and intestinal microbiota were recently revealed. Immune cells express nicotinic acetylcholine receptors (nAChRs), an NN target, suggesting CLO may disrupt the immune system. However, the relationship between CLO and atopic dermatitis (AD) is unknown. We administered a no-adverse-effect-level (NOAEL) dose of CLO to male NC/Nga mice with induced AD and measured, at three time points, key AD symptom indicators: epidermal thickening, mast cell number, total plasma IgE, and histamine levels. CLO increased total plasma IgE levels but reduced epidermal thickening, mast cell number, and plasma histamine levels in the early stages of AD. This demonstrates for the first time that CLO exposure inhibits AD's early symptoms.

  Feb. 2024, The Journal of veterinary medical science, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of exposure to the neonicotinoid pesticide clothianidin on α-defensin secretion and gut microbiota in mice.

  Sakura Yonoichi, Yukako Hara, Yuya Ishida, Asuka Shoda, Mako Kimura, Midori Murata, Sarika Nunobiki, Makiko Ito, Ayano Yoshimoto, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Yoshinori Ikenaka, Yuki Yokoi, Tokiyoshi Ayabe, Kiminori Nakamura, Nobuhiko Hoshi

  The mechanism by which the neonicotinoid pesticide clothianidin (CLO) disrupts the intestinal microbiota of experimental animals is unknown. We focused on α-defensins, which are regulators of the intestinal microbiota. Subchronic exposure to CLO induced dysbiosis and reduced short-chain fatty acid‒producing bacteria in the intestinal microbiota of mice. Levels of cryptdin-1 (Crp1, a major α-defensin in mice) in feces and cecal contents were lower in the CLO-exposed groups than in control. In Crp1 immunostaining, Paneth cells in the jejunum and ileum of the no-observed-adverse-effect-level CLO-exposed group showed a stronger positive signal than control, likely due to the suppression of Crp1 release. Our results showed that CLO exposure suppresses α-defensin secretion from Paneth cells as part of the mechanism underlying CLO-induced dysbiosis.

  Jan. 2024, The Journal of veterinary medical science, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Establishment of an organ culture system to maintain the structure of mouse Müllerian ducts during development

  Shiori KATO, Toshifumi YOKOYAMA, Taisei FUJIKAWA, Yusuke KIRIZUKI, Youhei MANTANI, Takanori MIKI, Nobuhiko HOSHI

  Japanese Society of Veterinary Science, 2024, Journal of Veterinary Medical Science, 86(3) (3), 300 - 307, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of exposure to the neonicotinoid pesticide clothianidin on mouse intestinal microbiota under unpredictable environmental stress.

  Sakura Yonoichi, Tetsushi Hirano, Yukako Hara, Yuya Ishida, Asuka Shoda, Mako Kimura, Midori Murata, Youhei Mantani, Toshifumi Yokoyama, Yoshinori Ikenaka, Nobuhiko Hoshi

  Recent research has demonstrated the toxicity of neonicotinoid pesticides (NNs) in mammals through their interaction with nicotinic acetylcholine receptors (nAChRs). These effects are reported to extend to the intestinal microbiota as well. In addition, environmental stress affects the expression of nAChRs, which may alter sensitivity to NNs. In this study, we analyzed the intestinal microbiota of mice exposed to clothianidin (CLO), a type of NN, under environmental stress, and aimed to clarify the effects of such combined exposure on the intestinal microbiota. C57BL/6N male mice (9 weeks old) were subchronically administered a no-observed-adverse-effect-level (NOAEL) CLO-mixed rehydration gel for 29 days and simultaneously subjected to chronic unpredictable mild stress (CUMS). After the administration period, cecum contents were collected and analyzed by 16S rRNA sequencing for intestinal microbiota. CLO exposure alone resulted in alterations in the relative abundance of Alistipes and ASF356, which produce short-chain fatty acids. The addition of CUMS amplified these changes. On the other hand, CLO alone did not affect the relative abundance of Lactobacillus, but the abundance decreased when CUMS was added. This study revealed that the combined exposure to CLO and stress not only amplifies their individual effects on intestinal microbiota but also demonstrates combined and multifaceted toxicities.

  Dec. 2023, Toxicology and applied pharmacology, 482, 116795 - 116795, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Direct diffusion of anti‐Müllerian hormone from both the cranial and caudal regions of the testis during early gonadal development in mice

  Shiori Kato, Toshifumi Yokoyama, Nobusuke Okunishi, Hiroto Narita, Taisei Fujikawa, Yusuke Kirizuki, Youhei Mantani, Takanori Miki, Nobuhiko Hoshi

  Abstract Background The Müllerian duct (MD), the primordium of the female reproductive tract, is also formed in males during the early stage of development, then regresses due to the anti‐Müllerian hormone (AMH) secreted from the testes. However, the detailed diffusion pathway of AMH remains unclear. We herein investigated the mechanism by which AMH reaches the middle region of the MD using an organ culture system. Results Injection of recombinant human AMH into the testis around the start of MD regression induced diffuse immunoreactivity in the mesonephros near the injection site. When the testis and mesonephros were cultured separately, the diameters of both cranial and middle MDs were significantly increased compared to the control. In the testis–mesonephros complex cultured by inhibiting the diffusion of AMH through the cranial region, the cranial MD diameter was significantly increased compared to the control, and there was no difference in middle MD diameter. Conclusions These results indicate that AMH, which infiltrates from the testis through the cranial region at physiological concentrations, induces regression of the cranial MD at the start of MD regression. They also indicate that AMH infiltrating through the caudal regions induces regression of the middle MD.

  Wiley, Oct. 2023, Developmental Dynamics, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Histological study on the reginal difference in the localization of mucosal enteric glial cells and their sheath structure in the rat intestine.

  Youhei Mantani, Nobuhiko Ohno, Tomohiro Haruta, Satoki Nakanishi, Rinako Morishita, Shota Murase, Toshifumi Yokoyama, Nobuhiko Hoshi

  The present study aimed to histologically clarify the regional specificity of the mucosal enteric glial cells (mEGCs) in the rat intestine with serial block-face scanning electron microscopy (SBF-SEM). SBF-SEM analysis with the ileum, the cecum and the descending colon revealed that mEGC nuclei were more abundant in the data stacks from the apical portion of the villus and the lateral portion of the crypt of the ileum. mEGCs exhibited a high rate of coverage over the nerve bundle around the lateral portion of the ileal crypt, but showed an extremely low level of coverage in the luminal portion of the cecum. These findings evidenced regional differences in the localization of mEGCs and in their sheath structure in the rat intestine.

  Aug. 2023, The Journal of veterinary medical science, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Transgenerational effects of developmental neurotoxicity induced by exposure to a no-observed-adverse-effect level (NOAEL) of neonicotinoid pesticide clothianidin.

  Asuka Shoda, Midori Murata, Mako Kimura, Yukako Hara, Sakura Yonoichi, Yuya Ishida, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Yoshinori Ikenaka, Nobuhiko Hoshi

  Neonicotinoid pesticides (NNs) transfer rapidly from mother to offspring, which exhibit neurobehavioral effects. However, no studies have investigated NNs' transgenerational effects. We exposed F0 generation mice (mothers) to a no-observed-adverse-effect level (NOAEL) of clothianidin (CLO) during gestation and lactation, and examined the adult neurobehavioral effects of three generations of offspring (F1, F2, F3). F1 had lower birth weight, decreased locomotor activity, and increased anxiety-like behavior. In F2, body weight was affected, and there was a decreasing trend in locomotor activity and an increasing trend in anxiety-like behavior. In F3, locomotor activity tended to increase. Thus, even when only the mothers were exposed, the effects of CLOs were still observed in F1, F2, and F3 but the effects became smaller.

  Aug. 2023, The Journal of veterinary medical science, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Diurnal changes in bacterial settlement on the Peyer’s patch and surrounding mucosa in the rat ileum and its effect against the intestinal immune system

  Youhei Mantani, Nanami Sakata, Naoto Kubota, Asaka Shimada, Satoki Nakanishi, Toshifumi Yokoyama, Nobuhiko Hoshi

  Springer Science and Business Media LLC, May 2023, Cell and Tissue Research

  [Refereed]

  Scientific journal


• Developmental stage-specific exposure and neurotoxicity evaluation of low-dose clothianidin during neuronal circuit formation.

  Asuka Shoda, Midori Murata, Mako Kimura, Yukako Hara, Sakura Yonoichi, Yuya Ishida, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Yoshinori Ikenaka, Yoshiaki Tabuchi, Nobuhiko Hoshi

  Neonicotinoid pesticides (NN) were recently reported to exhibit adverse effects in higher vertebrates. Moreover, NNs are routinely transferred from mother to offspring, raising concerns about their effects on future generations. The fetal and neonatal periods are the most critical to the formation of neural circuits in the brain through neurogenesis and differentiation, neuronal migration, axon guidance, and synaptogenesis. NN exposure throughout the fetal and neonatal periods was found to affect the neurobehavior of the offspring, but the stage-specific neurobehavioral effects are unclear. We exposed fetal and neonatal mice to a no-observed-adverse-effect level (NOAEL) of clothianidin (CLO) for 4 days during each of four developmental stages: neurite proliferation and differentiation (fetal days 9-12, CLO-1), neurite outgrowth (fetal days 15-18, CLO-2), synapse formation and astrocyte differentiation (days 1-4 after birth, CLO-3), and synapse remodeling (days 11-14 after birth, CLO-4). CLO's neurobehavioral effects were evaluated in juveniles and adults, revealing that CLO-1 and CLO-2 caused behavioral abnormalities in adult mice. CLO-3 significantly increased locomotor activity and decreased juvenile neurons in the hippocampal dentate gyrus in adulthood. Comprehensive gene analysis of CLO-3 revealed high expression of genes related to neurite outgrowth and axonal branching in the hippocampus in juveniles and adults. These results revealed developmental stage-specific effects of a NOAEL of CLO in the fetal and neonatal periods, suggesting that the susceptibility of the fetus and neonate to CLO varies by developmental stage.

  Mar. 2023, The Journal of veterinary medical science, 85(4) (4), 486 - 496, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Substrain differences in Sry expression at the stage of sex determination in C57BL/6 mouse strains.

  Hiroto Narita, Toshifumi Yokoyama, Nobusuke Okunishi, Shiori Kato, Taisei Fujikawa, Yusuke Kirizuki, Youhei Mantani, Takanori Miki, Nobuhiko Hoshi

  The expression of sex determining region of the Y chromosome (Sry) in the fetal gonads is important for male development. In a mouse model of disorders of sex development (C57BL/6 (B6)-XYPOS), the gonadal phenotype and the timing of Sry expression differ due to differences among the B6 substrain as the genetic background. Since differences in Sry expression among B6 substrains have been speculated, the present study examined Sry expression in B6J, B6JJmsSlc, and B6NCrl mice. These substrains differed in the number of Sry-expressing cells in the gonads of embryonic mice at each developmental stage, with B6NCrl having more than the other strains. The substrains differed also in the number of Sry-expressing cells between the left and right gonads, with B6J and B6NCrl, but not B6JJmsSlc, showing left gonad-dominant Sry expression. Substrain differences existed also in the distribution of Sry-expressing cells in the medial and lateral directions of gonads. In addition, in the left gonad-dominant Sry-expressing substrains B6J and B6NCrl, the medial and central regions of the left gonad had more Sry-expressing cells than those of the right gonad. Substrains of B6 mice have not always been considered in sex differentiation studies. In the present study, however, we observed substrain differences in the number of Sry-expressing cells, left-right distribution, and medial/lateral distribution during the early stages of gonadal development in B6 mice. Therefore, future studies on sex differentiation in B6 mice should consider substrain differences.

  Corresponding, Feb. 2023, The Journal of veterinary medical science, 85(4) (4), 507 - 514, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Neurotoxicity and behavioral disorders induced in mice by acute exposure to the diamide insecticide chlorantraniliprole.

  Mako Kimura, Asuka Shoda, Midori Murata, Yukako Hara, Sakura Yonoichi, Yuya Ishida, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Yoshinori Ikenaka, Nobuhiko Hoshi

  Diamide insecticides activate ryanodine receptors expressed in lepidopteran skeletal muscle and promote Ca2+ release in the sarcoplasmic reticulum, causing abnormal contractions and paralysis, leading to death of the pest. Although they had been thought not to act on nontarget organisms, including mammals, adverse effects on vertebrates were recently reported, raising concerns about their safety in humans. We investigated the neurotoxicity of the acute no-observed-adverse-effect level of chlorantraniliprole (CAP), a diamide insecticide, in mice using clothianidin (CLO), a neonicotinoid insecticide, as a positive control. The CLO-administered group showed decreased locomotor activities, increased anxiety-like behaviors, and abnormal human-audible vocalizations, while the CAP-administered group showed anxiety-like behaviors but no change in locomotor activities. The CAP-administered group had greater numbers of c-fos‒immunoreactive cells in the hippocampal dentate gyrus, and similar to the results in a CLO-administered group in our previous study. Blood corticosterone levels increased in the CLO-administered group but did not change in the CAP-administered group. Additionally, CAP was found to decreased 3-Methoxytyramine and histamine in mice at the time to maximum concentration. These results suggest that CAP-administered mice are less vulnerable to stress than CLO-administered mice, and the first evidence that CAP exposure increases neuronal activity and induces anxiety-like behavior as well as neurotransmitter disturbances in mammals.

  Feb. 2023, The Journal of veterinary medical science, 85(4) (4), 497 - 506, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Next-generation effects of fetal and lactational exposure to the neonicotinoid pesticide clothianidin on the immune system and gut microbiota.

  Midori Murata, Asuka Shoda, Mako Kimura, Yukako Hara, Sakura Yonoichi, Yuya Ishida, Youhei Mantani, Toshifumi Yokoyama, Eiko Matsuo, Tetsushi Hirano, Nobuhiko Hoshi

  Recently, the effects of exposure to clothianidin (CLO) on the thymus and gut microbiota have become clear, but no report has examined its next-generation impacts. Pregnant C57BL/6N mice were administered a no-observed-adverse-effect-level dose of CLO until weaning. We examined CLO's effects on the gut microbiota and immune organs of dams and their 3- and 10-week-old male offspring. CLO administration led to several alterations of the top 30 bacterial genera in the gut microbiota in dams and 3-week-old mice. Compared to controls, 10-week-old mice had more thymic Hassall's corpuscles, and both dams and 10-week-old mice had fewer macrophages. These results suggest that fetal and lactational CLO exposure may affect the immune system and gut microbiota of the next generation.

  Feb. 2023, The Journal of veterinary medical science, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Histological study on regional specificity of the mucosal nerve network in the rat large intestine.

  Satoki Nakanishi, Youhei Mantani, Nobuhiko Ohno, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi

  Our previous studies and others have revealed detailed characteristics of the mucosal nerve network in the small intestine, but much remains unknown about the corresponding network in the large intestine. We herein investigated regional differences in the expression of neurochemical markers, the nerve network structure, and the cells in contact with nerve fibers by histological analysis using both immunohistochemistry and serial block-face scanning electron microscopy (SBF-SEM). Immunohistochemistry revealed that immunopositive structures for protein gene product 9.5, vasoactive intestinal peptide (VIP), calretinin and vesicular acetylcholine transporter were more prevalent in the lamina propria of the ascending colon than the cecum and descending colon (DC). There was no significant difference in the frequency of most neurochemical markers between the cecum and DC, but the frequencies of VIP+ structures were higher in the cecum than in the DC. SBF-SEM analysis showed that the nerve network structure was more developed on the luminal side of the DC than the cecum. The cells that nerve fibers abundantly contacted were subepithelial and lamina propria fibroblast-like cells and macrophages. In addition, nerve fibers in the cecum were in more frequent contact with immune cells such as macrophages and plasma cells than nerve fibers in the DC. Thus, the present histological analysis suggested that the mucosal nerve network in the large intestine possessed both regional universality and various specificities, and revealed the intimate relationship between the nerve network and immune cells, especially in the cecum.

  Dec. 2022, The Journal of veterinary medical science, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Quantitative elucidation of the transfer of the neonicotinoid pesticide clothianidin to the breast milk in mice.

  Asuka Shoda, Misaki Nishi, Midori Murata, Youhei Mantani, Toshifumi Yokoyama, Tetsushi Hirano, Yoshinori Ikenaka, Nobuhiko Hoshi

  Neonicotinoid pesticides (NNs) have been reported to have neurobehavioral effects on offspring after fetal and lactational exposure. In this study, clothianidin (CLO), an NN, was administered orally as a single dose (6.5mg/kg: 1/10 of the no-observed-adverse-effect level in the current Pesticide Evaluation Report) to 10-day post-partum ICR mice, and CLO and its metabolites were quantified using liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-ESI/MS/MS) after collecting maternal breast milk and blood samples over time (1, 3, 6, 9, 12, and 24h after administration). CLO and its metabolite desmethyl-CLO (dm-CLO) were detected in the breast milk at 1h after the administration, and their concentrations were significantly higher than those in blood at all time points. The concentrations of CLO and dm-CLO in the breast milk were at their highest levels at 1 and 3h, respectively, and then decreased over time to become almost undetectable at 24h after the administration. These results show that CLO is metabolized in the mother's body and is rapidly transferred to and concentrated in the breast milk. Since CLO concentrations in breast milk are higher than those in the blood, there is concern about the effects of CLO during lactation.

  Oct. 2022, Toxicology letters, 373, 33 - 40, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Sex-specific behavioral effects of acute exposure to the neonicotinoid clothianidin in mice.

  Shizuka Kubo, Tetsushi Hirano, Yuka Miyata, Shuji Ohno, Kanoko Onaru, Yoshinori Ikenaka, Shouta M M Nakayama, Mayumi Ishizuka, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi

  Although neonicotinoids are among the major classes of pesticides that affect mammalian nervous systems, little is known about sex differences in their effects. This study aimed to examine whether the neurobehavioral effects of a neonicotinoid, clothianidin (CLO), differed between sexes. Male and female C57BL/6 N mice were orally administered CLO (5 or 50 mg/kg) at or below the chronic no-observed-adverse-effect-level (NOAEL) and subjected to behavioral tests of emotional and learning functions. Changes in neuroactivity in several brain regions and the concentrations of CLO and its metabolites in blood and urine were measured. Acute CLO exposure caused sex-related behavioral effects; decreases in locomotor activities and elevation of anxiety-like behaviors were more apparent in males than in females. In addition, male-specific impairment of short- and long-term learning memory by CLO exposure was observed in both the novel recognition test and the Barnes maze test. Male-dominant increases in the number of c-fos positive cells were observed in the paraventricular thalamic nucleus in the thalamus and in the dentate gyrus in the hippocampus, which are related to the stress response and learning function, respectively. The concentrations of CLO and most metabolites in blood and urine were higher in males. These results support the notion that male mice are more vulnerable than females to the neurobehavioral effects of CLO and provide novel insights into the risk assessment of neonicotinoids in mammalian neuronal function.

  Oct. 2022, Toxicology and applied pharmacology, 456, 116283 - 116283, English, International magazine

  [Refereed]

  Scientific journal


• Region specificity of fibroblast-like cells in the mucosa of the rat large intestine.

  Saki Tamura, Youhei Mantani, Satoki Nakanishi, Nobuhiko Ohno, Toshifumi Yokoyama, Nobuhiko Hoshi

  Our previous studies using immunohistochemistry and serial block-face scanning electron microscopy (SBF-SEM) clarified that fibroblast-like cells (FBLCs) in the rat ileal mucosa are classifiable into several subtypes, but their characteristics throughout the large intestine remain unknown. In this study, we investigated the region-specific characteristics of FBLCs in the rat large intestine using histological analysis including SBF-SEM. Immunohistochemistry revealed that CD34+CD31- FBLCs were localized in the lamina propria beneath the crypt bases throughout the large intestine and were more abundant in the descending colon than in the other regions. In addition, platelet-derived growth factor receptor α (PDGFRα)+ FBLCs were ubiquitously present just below the epithelium throughout the large intestine, and those at the crypt base were slightly more abundant in the descending colon than in the other regions. SBF-SEM analysis revealed that there were two types of FBLCs around the crypt base in both the cecum and the descending colon: sub-epithelial FBLCs localizing just beneath the epithelium in the manner of PDGFRα+ FBLCs, and lamina propria FBLCs localizing farther away from the epithelium than sub-epithelial FBLCs in the manner of CD34+CD31- FBLCs. The lamina propria FBLCs were closely apposed to various immune cells in the lamina propria, and their endoplasmic reticulum in the descending colon exhibited various dilatation levels, unlike that in the cecum. These findings indicate that FBLCs, especially around the crypt base, differed in each region of the large intestine with respect to localization, abundance, and ultrastructure, which could lead to the region-specific microenvironment around the crypt base.

  Sep. 2022, Cell and tissue research, 389(3) (3), 427 - 441, English, International magazine

  [Refereed]

  Scientific journal


• Histological study of diurnal changes in bacterial settlement in the rat alimentary tract

  Nanami Sakata, Youhei Mantani, Satoki Nakanishi, Rinako Morishita, Toshifumi Yokoyama, Nobuhiko Hoshi

  Springer Science and Business Media LLC, Apr. 2022, Cell and Tissue Research

  [Refereed]

  Scientific journal


• Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy <i>in vivo</i> imaging

  Misaki NISHI, Shouta SUGIO, Tetsushi HIRANO, Daisuke KATO, Hiroaki WAKE, Asuka SHODA, Midori MURATA, Yoshinori IKENAKA, Yoshiaki TABUCHI, Youhei MANTANI, Toshifumi YOKOYAMA, Nobuhiko HOSHI

  Japanese Society of Veterinary Science, 2022, Journal of Veterinary Medical Science, 84(4) (4), 585 - 592

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Aging-related changes in the sensitivity of behavioral effects of the neonicotinoid pesticide clothianidin in male mice.

  Tetsushi Hirano, Yuka Miyata, Shizuka Kubo, Shuji Ohno, Kanoko Onaru, Mizuki Maeda, Sayaka Kitauchi, Misaki Nishi, Yoshiaki Tabuchi, Yoshinori Ikenaka, Takahiro Ichise, Shouta M M Nakayama, Mayumi Ishizuka, Koji Arizono, Keisuke Takahashi, Keisuke Kato, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi

  Neonicotinoids, which act as agonists of the nicotinic acetylcholine receptors of insects, are widely used pesticides worldwide. Although epidemiological studies revealed that the detection amounts of neonicotinoids in urine are higher in the elderly population than other age-groups, there is no available information regarding the risks of neonicotinoids to older mammals. This study was aimed to investigate aging-related differences in the behavioral effects of the neonicotinoid pesticide clothianidin (CLO). We acutely administered a sub-NOAEL level (5 mg/kg) of CLO to adult (12-week-old) and aging (90-week-old) mice and conducted four behavioral tests focusing on the emotional behavior. In addition, we measured the concentrations of CLO and its metabolites in blood, brain and urine. There were age-related changes in most parameters in all behavioral tests, and CLO significantly decreased the locomotor activity in the open field test and elevated plus-maze test in the aging group, but not in the adult group. The concentrations of most CLO and its metabolites were significantly higher in the blood and brain and were slightly lower in the urine in the aging group compared to the adult group. These findings should contribute to our understanding of age-related differences in the adverse effects of neonicotinoids in mammals.

  May 2021, Toxicology letters, 342, 95 - 103, English, International magazine

  [Refereed]

  Scientific journal


• Ultrastructural and phenotypical diversity of macrophages in the rat ileal mucosa.

  Youhei Mantani, Tomohiro Haruta, Satoki Nakanishi, Nanami Sakata, Hideto Yuasa, Toshifumi Yokoyama, Nobuhiko Hoshi

  Several types of macrophages have been reported in the intestinal mucosa, but their histological localization remains ambiguous. Here, we obtained detailed information about ultrastructural and phenotypical diversity of macrophage-like cells (MLCs) in the rat ileal mucosa using immunofluorescent analysis and serial block-face scanning electron microscopy (SBF-SEM). The results revealed that the cells immunopositive for CD68, the pan-macrophage marker, included CD163-CD4+, CD163+CD4+, and CD163-CD4- cells in the lamina propria (LP) of the intestinal villus and around the crypt. CD68+CD4+CD163- cells seemed to be preferentially localized in the intestinal villus, whereas CD68+CD163+CD4+ cells were frequently localized around the crypt. SBF-SEM analysis identified three types of MLCs in the ileal mucosa, which were tentatively named types I-III MLC based on aspects of the 3D-ultrastructure, such as the localization, quantity of lysosomes, endoplasmic reticulum, and exoplasm. Type I and II MLCs were localized in the villous LP, while type III MLCs were localized around the crypt, although type II MLCs were a minor population. All three MLC types extended their cellular processes into the epithelium, with type I MLCs showing the greatest abundance of extended processes. Type I MLCs in the upper portion of the intestinal villus showed a higher level of attachment to intraepithelial lymphocytes (IELs) compared to type III MLCs around the crypt. These findings suggest that macrophages of the rat ileal mucosa differed by region along the longitudinal axis of the villous tip-crypt from the perspective of ultrastructure, cellular composition, localization, and interactions with IELs.

  May 2021, Cell and tissue research, English, International magazine

  [Refereed]

  Scientific journal


• Effects of in utero and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid clothianidin on the reproductive organs of female mice.

  Sayaka Kitauchi, Mizuki Maeda, Tetsushi Hirano, Yoshinori Ikenaka, Misaki Nishi, Asuka Shoda, Midori Murata, Youhei Mantani, Toshifumi Yokoyama, Yoshiaki Tabuchi, Nobuhiko Hoshi

  Recently, developmental exposure to clothianidin (CLO) has been shown to cause reproductive toxicity in male mice, but the effects in female mice remain to be clarified. Pregnant C57BL/6N mice were given a no-observed-adverse-effect-level (NOAEL) dose of CLO until weaning. We then examined ovaries of 3- or 10-week-old female offspring. In the CLO-administered group, morphological changes, a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 (GPx4), and activation of genes in the steroid hormone biosynthesis pathway were observed in 3-week-old mice, and decreases of GPx4 immunoreactivity, 17OH-progesterone and corticosterone levels were observed in 10-week-old mice, along with high rates of infanticide and severe neglect, providing new evidence that developmental exposure to CLO affects juvenile and adult mice differently.

  Apr. 2021, The Journal of veterinary medical science, 83(4) (4), 746 - 753, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Fetal and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid pesticide clothianidin inhibits neurogenesis and induces different behavioral abnormalities at the developmental stages in male mice.

  Mizuki Maeda, Sayaka Kitauchi, Tetsushi Hirano, Yoshinori Ikenaka, Misaki Nishi, Asuka Shoda, Midori Murata, Youhei Mantani, Yoshiaki Tabuchi, Toshifumi Yokoyama, Nobuhiko Hoshi

  Recently, it has been reported that neonicotinoid pesticides (NNs) are transferred from mother to child and are assumed to affect the next generation, but the behavioral effects of NN exposure at different developmental stages have not been investigated. We exposed mice to no-observed-adverse-effect level (NOAEL) doses of clothianidin (CLO) during the fetal and lactational period, and then evaluated the neurobehavioral effects in juvenile and adult mice. Significant increases in anxiety-like behavior and locomotor activity were observed in juveniles and adults, respectively, and neuronal activity and neurogenesis in the hippocampal dentate gyrus were affected in both stages. These results suggest that fetal and lactational exposure to CLO may inhibit neurogenesis and cause different behavioral abnormalities at different developmental stages.

  Apr. 2021, The Journal of veterinary medical science, 83(3) (3), 542 - 548, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Influence of acute exposure to a low dose of systemic insecticide fipronil on locomotor activity and emotional behavior in adult male mice.

  Mizuki Maeda, Toshifumi Yokoyama, Sayaka Kitauchi, Tetsushi Hirano, Youhei Mantani, Yoshiaki Tabuchi, Nobuhiko Hoshi

  Fipronil (FPN) is a systemic insecticide that antagonizes the gamma-aminobutyric acid type A (GABAA) receptors in insects. Recently, adverse effects of FPN on mammals have been reported, but most of those were caused by high doses of FPN and additives in the products. We investigated the effects of low-dose pure FPN on the emotional behavior of mice. Nine-week-old male mice conducted behavioral tests 24 hr after FPN administration by gavage at doses of 0.05 or 5 mg/kg based on the no-observed-effect level (NOEL), showed a significant increase in locomotor activity and dose-dependent responses on the time they spent in the central zone in the open field test. Pure FPN below the NOEL dose may affect the emotional behavior of mice.

  Corresponding, Dec. 2020, The Journal of veterinary medical science, 83(2) (2), 344 - 348, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Morphological and phenotypical diversity of eosinophils in the rat ileum.

  Masaya Arai, Youhei Mantani, Satoki Nakanishi, Tomohiro Haruta, Miho Nishida, Hideto Yuasa, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Eosinophils are abundantly present in intestinal mucosa. However, the morphological characteristics of their cellular population are still largely unknown. In this study, we examine their characteristics in the rat ileal mucosa using histological and ultrastructural methods. The results indicated that ileal eosinophils could be distinguished into two main groups based on their nuclear shapes and distribution: eosinophils with spheric or reniform nuclei mainly localized in the villous region and eosinophils with annular or bacilliform nuclei as the major population around crypts. Immunohistochemical analysis revealed that all eosinophils in the lamina propria (LP) were immunopositive for CD11b, whereas eosinophils in LP of the intestinal villus but not those in LP around the crypt, were immunopositive for CD11c. Three-dimensional ultrastructural analysis using serial block-face scanning electron microscopy showed that the eosinophils with spheric or reniform nuclei were abundant in the upper portions of the intestinal villus, whereas those with annular nuclei were abundant in the lower portions of the intestinal villus and around crypts. The eosinophils with spheric or reniform nuclei possessed broader cellular bodies with greater abundance of surface projections compared with those with annular nuclei. Eosinophils in the upper portions of intestinal villus frequently extended their cellular bodies into the intraepithelial space. The number of total and eosinophil-specific granules was positively correlated with the minor axis of the nuclear holes in the annular nuclei. These data suggest that ileal eosinophils exhibit not homogenous but rather diverse characteristics, possible due to the mixture of eosinophils at different maturation and/or activation stages.

  Sep. 2020, Cell and tissue research, 381(3) (3), 439 - 450, English, International magazine

  [Refereed]

  Scientific journal


• Three-dimensional analysis of neural connectivity with cells in rat ileal mucosa by serial block-face scanning electron microscopy.

  Satoki Nakanishi, Youhei Mantani, Tomohiro Haruta, Toshifumi Yokoyama, Nobuhiko Hoshi

  The comprehensive targets of innervation in the intestinal mucosa are unknown, partly because of the diversity of cell types and the complexity of the neural circuits. Herein, we investigated the comprehensive targets of neural connectivity and analyzed the precise characteristics of their contact structures in the mucosa of rat ileum. We examined target cells of neural connections and the characteristics of their contact structures by serial block-face scanning electron microscopy at four portions of the rat ileal mucosa: the apical and basal portions in the villi, and the lateral and basal portions around/in the crypts. Nerve fibers were in contact with several types of fibroblast-like cells (FBLCs), macrophage-like cells, eosinophils, lymphocyte-like cells, and other types of cells. The nerve fibers almost always ran more inside of lamina propria than subepithelial FBLC, and thus contacts with epithelial cells were very scarce. The contact structures of the nerve fibers were usually contained synaptic vesicle-like structures, and we classified them into patterns based on the number of nerve fiber contacting the target cells at one site, the maximum diameter of the contact structures, and the relationship between nerve fibers and nerve bundles. The contact structures for each type of cells occasionally dug into the cellular bodies of the target cells. We revealed the comprehensive targets of neural connectivity based on the characteristics of contact structures, and identified FBLCs, immunocompetent cells, and eosinophils as the candidate targets for innervation in the rat ileal mucosa.

  Jul. 2020, The Journal of veterinary medical science, 82(7) (7), 990 - 999, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads.

  Chinatsu Hasegawa, Toshifumi Yokoyama, Yuria Umemura, Kohei Kawanishi, Yuuka Miura, Nanako Takada, Shuji Ohno, Kanoko Onaru, Takuya Omotehara, Tetsushi Hirano, Yohei Mantani, Takanori Miki, Nobuhiko Hoshi

  Organ culture systems are useful for elucidating the process of testicular differentiation from mammalian undifferentiated genetically male gonads, as they permit various experiments, including experiments involving the control of gene expression. However, without addition of testicular differentiation-related factors, it is difficult to induce the formation of testis cord from immature gonads by a time point earlier 12 tail somites (ts) that corresponding to 11.0 days post coitum (dpc). In this study, we attempted to establish an organ culture system that induces testis formation from immature gonads (around 8 ts: 10.5 dpc) just before Sry (sex-determining region of the Y chromosome) expression. A paired gonad-mesonephros complex of around 8 ts was placed in the groove of an agarose gel block and put the semi-cylindrical piece of agarose gel to maintain the gonad morphology. The gonads were cultured in the gas phase for 96 hr. As a result, testis cord-like structures appeared in many genetically male gonads. Cells expressing the Sertoli cell markers Sox9 (SRY-box 9) and Amh (anti-Müllerian hormone) were observed, while granulosa cell marker Foxl2 (forkhead box L2) was not detected. In addition, Sox9- and Amh-expressing cells were observed throughout the entire gonad in many individuals. Amh mRNA expression was also upregulated. Surprisingly, formation of a partial testicular structure was observed from more immature gonads (6 ts). These results show that our gonadal organ culture system is useful for elucidating the regulation mechanism of Sry expression in undifferentiated bipotential gonads.

  Apr. 2020, The Journal of veterinary medical science, 82(4) (4), 414 - 421, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Quantitative elucidation of maternal-to-fetal transfer of neonicotinoid pesticide clothianidin and its metabolites in mice.

  Shuji Ohno, Yoshinori Ikenaka, Kanoko Onaru, Shizuka Kubo, Nanami Sakata, Tetsushi Hirano, Youhei Mantani, Toshifumi Yokoyama, Keisuke Takahashi, Keisuke Kato, Koji Arizono, Takahiro Ichise, Shouta M M Nakayama, Mayumi Ishizuka, Nobuhiko Hoshi

  Neonicotinoids (NNs), a widely used class of systemic pesticides, are regarded as exhibiting selective toxicity in insects. However, NNs are suspected of exerting adverse effects on mammals as well, including humans. To date, only adult male animal models have been subjected to general toxicity studies of NNs; fetuses have yet to be considered in this context. Here, we focused on the NN clothianidin (CLO) for the first quantitative LC-MS/MS analysis of maternal-to-fetal transfer and residual property of once-daily (single or multiple days), orally administered CLO and its metabolites in mice. The results revealed the presence of CLO and its five metabolites at approximately the same respective blood levels in both dams and fetuses. In the dams, CLO showed a peak value 1 h after administration, after which levels rapidly decreased at 3 and 6 h. In the fetuses of each group, levels of CLO were almost the same as those observed in the corresponding dams. The present results clearly demonstrated rapid passage of CLO through the placental barrier. However, metabolite-dependent differences observed in blood pharmacokinetics and residual levels. This is the first quantitative demonstration of the presence of CLO and its metabolites in fetal mouse blood.

  Elsevier BV, Apr. 2020, Toxicology letters, 322, 32 - 38, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Combined exposure to dinotefuran and chronic mild stress counteracts the change of the emotional and monoaminergic neuronal activity induced by either exposure singly despite corticosterone elevation in mice.

  Tadashi Takada, Naoki Yoneda, Tetsushi Hirano, Kanoko Onaru, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Collins Nimako, Mayumi Ishizuka, Yoshinori Ikenaka, Nobuhiko Hoshi

  Dinotefuran (DIN) belongs to the neonicotinoids (NNs), a class of globally applied pesticides originally developed to exhibit selective toxicity in insects. However, several reports have suggested that NNs also exert neurotoxic effects in mammals. We previously demonstrated neurobehavioral effects of DIN on mice under non-stressful conditions. For further toxicity assessments in the present study, we investigated the effects of DIN on mice exposed to stressful conditions. After subacutely administering a no-observed-effect-level (NOEL) dose of DIN and/or chronic unpredictable mild stress (CUMS) to mice, we conducted three behavioral tests (i.e., open field test [OFT], tail suspension test [TST] and forced swimming test [FST]). In addition, serotonin (5-HT) and tryptophan hydroxylase 2 (TPH2) of the dorsal raphe nuclei (DRN) and median raphe nuclei (MRN) and tyrosine hydroxylase (TH) of the ventral tegmental area and substantia nigra (SN) were evaluated immunohistochemically. A NOEL dose of DIN or CUMS alone increased of the total distance in OFT, decreased or increased the immobility time in TST or FST, respectively, and increased the positive intensity of 5-HT and TPH2 in the DRN/MRN, and TH in the SN. These changes were suppressed under the conditions of combined exposure to DIN and CUMS, though the blood corticosterone level was increased depending on the blood DIN values and the presence of CUMS. The present study suggests the multifaceted toxicity of the neurotoxin DIN.

  Mar. 2020, The Journal of veterinary medical science, 82(3) (3), 350 - 359, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Immunotoxicity evaluation by subchronic oral administration of clothianidin in Sprague-Dawley rats.

  Kanoko Onaru, Shuji Ohno, Shizuka Kubo, Satoki Nakanishi, Tetsushi Hirano, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi

  Neonicotinoid pesticides (NNs) act as agonists on nicotinic acetylcholine receptors (nAChRs) of insects, and there have been concerns about the effects of NNs on the health of mammals. Since nAChRs are expressed in immune cells, it is possible that NNs disturb the immune system. However, few reports have examined the immunotoxicity of clothianidin (CLO), a widely-used NN. Here, we report the effects of CLO on immune organs and type IV allergic reactions in ear auricles. We orally administered CLO at 0, 30 and 300 mg/kg/day (CLO-0, 30 and 300) to Sprague-Dawley rats for 28 days. The effects were evaluated by organ and body weights, histopathology, and immunohistochemistry (TCRαβ, CD4, CD8, CD11b, CD68, CD103). In addition, some cecal contents were subjected to preliminary gut microbiota analysis, because microbiota contribute to host homeostasis, including the immunity. Our results showed loose stool, suppression of body weight gain, significant changes in organ weights (thymus: decreased; liver: increased) and changes of the gut microbiota in the CLO-300 group. There were no obvious histopathological changes in immune organs. Granulomas of the ear auricles were found in one rat of each of the CLO-30 and 300 groups, but CLO had no apparent effect on the thickness or immunohistochemistry in the ear auricles. We present new evidence that CLO affects the thymus and intestine, and might enhance the local inflammatory response. These findings should contribute to the appropriate evaluation of the safety of NNs in the future.

  Mar. 2020, The Journal of veterinary medical science, 82(3) (3), 360 - 372, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Growth and neurite stimulating effects of the neonicotinoid pesticide clothianidin on human neuroblastoma SH-SY5Y cells.

  Tetsushi Hirano, Satsuki Minagawa, Yukihiro Furusawa, Tatsuya Yunoki, Yoshinori Ikenaka, Toshifumi Yokoyama, Nobuhiko Hoshi, Yoshiaki Tabuchi

  Neonicotinoids are one of most widely used pesticides targeting nicotinic acetylcholine receptors (nAChRs) of insects. Recent epidemiological evidence revealed increasing amounts of neonicotinoids detected in human samples, raising the critical question of whether neonicotinoids affect human health. We investigated the effects of a neonicotinoid pesticide clothianidin (CTD) on human neuroblastoma SH-SY5Y cells as in vitro models of human neuronal cells. Cellular and functional effects of micromolar doses of CTD were evaluated by changes in cell growth, intracellular signaling activities and gene expression profiles. We examined further the effects of CTD on neuronal differentiation by measuring neurite outgrowth. Exposure to CTD (1-100 μM) significantly increased the number of cells within 24 h of culture. The nAChRs antagonists, mecamylamine and SR16584, inhibited this effect, suggesting human α3β4 nAChRs could be targets of neonicotinoids. We observed a transient intracellular calcium influx and increased phosphorylation of extracellular signal-regulated kinase 1/2 shortly after exposure to CTD. Transcriptome analysis revealed that CTD down-regulated genes involved in neuronal function (e.g., formation of filopodia and calcium ion influx) and morphology (e.g., axon guidance signaling and cytoskeleton signaling); these changes were reflected by a finding of increased neurite length during neuronal differentiation. These findings provide novel insight into the potential risks of neonicotinoids to the human nervous system.

  Nov. 2019, Toxicology and applied pharmacology, 383, 114777 - 114777, English, International magazine

  [Refereed]

  Scientific journal


• Retinoic acid-stimulated ERK1/2 pathway regulates meiotic initiation in cultured fetal germ cells.

  Kim SM, Yokoyama T, Ng D, Ulu F, Yamazaki Y

  Nov. 2019, PLoS One., 14(11) (11), e0224628

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Genetic differences between C57BL/6 substrains affect the process of testis differentiation in YPOS mice.

  Toshifumi Yokoyama, Yuuka Miura, Anzu Yamamoto, Chinatsu Hasegawa, Kohei Kawanishi, Nanako Takada, Takuya Omotehara, Tetsushi Hirano, Yohei Mantani, Takanori Miki, Nobuhiko Hoshi

  C57BL/6J-XYPOS (B6J-XYPOS) mice, which have the Y chromosome derived from Mus musculus poschiavinus on a B6J genetic background, form ovotestes or ovaries. Previously, we replaced the genetic background of B6J-XYPOS mice with B6N and found that individuals with testes also appeared in addition to those with ovaries or ovotestes. To investigate the effect of the B6J genetic sequence on the testis differentiation, the genetic background of B6N-XYPOS mice was replaced with B6J again. The recovery of the B6J genetic background significantly decreased the incidence of testes; only ovaries developed. These results indicate that the testicular differentiation process tends to be perturbed especially in the B6J substrain. This shows the importance of substrain differences in mice usually treated as B6 collectively.

  Apr. 2019, The Journal of veterinary medical science, 81(4) (4), 608 - 611, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Three-dimensional analysis of fibroblast-like cells in the lamina propria of the rat ileum using serial block-face scanning electron microscopy

  MANTANI YOUHEI, HARUTA TOMOHIRO, NISHIDA MIHO, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  Serial block-face scanning electron microscopy (SBF-SEM) is useful for three-dimensional observation of tissues or cells at high-resolution. In this study, SBF-SEM was used to three-dimensionally analyze the characteristics of fibroblast-like cells (FBLCs) in the rat ileal lamina propria (LP). The results revealed that FBLCs in LP could be divided into four types, tentatively named FBLC type I-IV, based on the external cellular appearance, abundance or shape of each organelle, detailed distribution in the LP and relationship with surrounding cells. FBLC-I and -II localized around the intestinal crypt (InC), FBLC-III localized from the lateral portion of InC to the apical portion of the intestinal villus (InV), and FBLC-IV localized in the InV. FBLC-I, -II and -III, but not FBLC-IV, localized beneath the epithelium. FBLC-II possessed thin lamellar-shaped endoplasmic reticula, whereas FBLC-I possessed expanded endoplasmic reticula that occasionally showed a spherical shape. FBLC-III and -IV possessed a cytoplasmic region with high-electron density and no organelles immediately beneath the cellular membrane; this region was found at the epithelial sides in FBLC-III and scattered in FBLC-IV. FBLC-IV were in constant close proximity to villous myocytes throughout the InV and also in contact with FBLC-III especially in the apical portion of the InV. FBLC-I, -II and -IV, but not -III, were constantly found to be in contact with various immunocompetent cells in the LP. Three-dimensional analysis using SBF-SEM indicates that four types of FBLC localized in the rat ileal LP.

  2019, The Journal of Veterinary Medical Science, 81(3) (3), 454 - 465, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of the expansion of bacterial colonies into the intervillous spaces on the localization of several lymphocyte lineages in the rat ileum

  YUASA HIDETO, MANTANI YOUHEI, MIYAMOTO KAZUKI, NISHIDA MIHO, ARAI MASAYA, TSURUTA HIROKI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  The effect of bacterial colonies expanded into the intervillous spaces on the localization of several lymphocyte lineages was immunohistochemically investigated in two types of mucosa: ordinary mucosa of rat ileum, which consists of mucosa without any mucosal lymphatic tissue; and follicle-associated mucosa (FAM), which accompanies the parafollicular area under the muscularis mucosae in the rat ileal Peyer's patch. The results showed that bacterial colonies in the intervillous spaces induced increased populations of CD8+ cells in the epithelium of the intestinal villus in ordinary mucosa (IV) and intestinal villus in FAM (IV-FAM). Bacterial colonies in the intervillous spaces were also associated with increased numbers of IgA+ cells, which were mainly localized in the lamina propria of basal portions of IV and IV-FAM, and with expanded localization of IgA+ cells into the villous apex in both IV and IV-FAM. Moreover, IgA+ cells around the intestinal crypts adjacent to IV or IV-FAM were also increased in response to bacterial colonies. In the IV-FAM, but not IV, L-selectin+ cells, which were found to be immunopositive for TCRαβ or CD19, were drastically increased in the lamina propria from the crypt to middle portion of IV-FAM and in the lumen of central lymph vessel of IV-FAM in response to the bacterial colonies in the intervillous spaces. These findings revealed that the expansion of bacterial colonies into the intervillous spaces accompanies the change of histological localization of the lymphocyte lineage in both the ordinary mucosa and FAM.

  2019, The Journal of Veterinary Medical Science, 81(4) (4), 555 - 566, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Identification of reference genes for quantitative PCR analyses in developing mouse gonads.

  Toshifumi Yokoyama, Takuya Omotehara, Tetsushi Hirano, Naoto Kubota, Shogo Yanai, Chinatsu Hasegawa, Tadashi Takada, Yohei Mantani, Nobuhiko Hoshi

  Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.

  Nov. 2018, The Journal of veterinary medical science, 80(10) (10), 1534 - 1539, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Ultrastructural and Immunohistochemical Study on the Lamina Propria Cells Beneath Paneth Cells in the Rat Ileum.

  Youhei Mantani, Miho Nishida, Kyouji Yamamoto, Kazuki Miyamoto, Hideto Yuasa, Natsumi Masuda, Takuya Omotehara, Hiroki Tsuruta, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Paneth cells secrete bactericidal substances in response to bacterial proliferation on the mucosal surface without directly contacting bacteria. However, the induction mechanism of this transient secretion has not been clarified, although nervous system and/or immunocompetent cells in the lamina propria (LP) might be involved. In this study, we ultrastructurally and immunohistochemically investigated which LP cells are localized beneath Paneth cells and examined the relationship between the Paneth cell-derived cellular processes which extended into the LP and the LP cells. The results showed that various cells-including blood capillary, subepithelial stromal cell, and nerve fiber-were present in the LP beneath Paneth cells. Endothelial cells of blood capillary were the cells most frequently found in this location; they were situated within 1 μm of the Paneth cells and possessed fenestration on the surfaces adjacent to Paneth cells. The Paneth cells rarely extended the cellular processes toward the LP across the basal lamina. Most of the cellular processes of Paneth cells contacted the subepithelial stromal cells. Immunohistochemistry revealed that the CD34+ CD31- αSMA- stromal cells preferentially localized in the LP beneath the intestinal crypt base, while PDGFRαhi αSMA+ stromal cells mainly localized around the lateral portions of the intestinal crypt and PDGFRαhi αSMA- stromal cells localized in the intestinal villus. From these findings, the existence of blood capillaries beneath Paneth cells might reflect the active exocrine function of Paneth cells. Furthermore, subepithelial stromal cells, probably with a CD34+ CD31- αSMA- PDGFRα-/lo phenotype, beneath the crypt base might affect Paneth cell activity by interacting with their cellular processes. Anat Rec, 301:1074-1085, 2018. © 2018 Wiley Periodicals, Inc.

  Jun. 2018, Anatomical record (Hoboken, N.J. : 2007), 301(6) (6), 1074 - 1085, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The mechanisms underlying the effects of AMH on Müllerian duct regression in male mice.

  Anzu Yamamoto, Takuya Omotehara, Yuuka Miura, Tadashi Takada, Naoki Yoneda, Tetsushi Hirano, Youhei Mantani, Hiroshi Kitagawa, Toshifumi Yokoyama, Nobuhiko Hoshi

  Anti-Müllerian hormone (AMH) produced in the developing testis induces the regression of the Müllerian duct, which develops into the oviducts, uterus and upper vagina. In our true hermaphrodite mouse with an ovary on one side and a testis on the other (O/T), the oviduct and uterus are present only on the ovary side, and nothing derived from the Müllerian duct is present on the testis side. Here, we investigate the mechanism underlying the unilateral Müllerian duct regression and the mode of AMH signaling, by performing immunohistology, Western blotting, and organ culture analyses. The histological analysis revealed that during the start of the Müllerian duct regression, the duct in the O/T mice was clearly regressed on the AMH-positive testis side compared to the AMH-negative ovary side. The immunohistochemistry showed a diffuse immunoreaction of AMH in the interstitium surrounding the testis cord and boundary region between the testis and mesonephros, especially in the cranial portion. Western blotting revealed that the amount of AMH in the cranial half of the mesonephros was larger than that in the caudal half. AMH injected into the gonads in organ culture induced the regression of the Müllerian duct via the interstitium of the organ. These results suggest that AMH acts on the Müllerian duct in male mice by exuding into the interstitium surrounding the testis cord and infiltrating through the cranial region from the testis to the mesonephros.

  Apr. 2018, The Journal of veterinary medical science, 80(4) (4), 557 - 567, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Verification of the causal relationship between subchronic exposures to dinotefuran and depression-related phenotype in juvenile mice

  Tadashi Takada, Naoki Yoneda, Tetsushi Hirano, Shogo Yanai, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Nobuhiko Hoshi

  Japanese Society of Veterinary Science, Apr. 2018, Journal of Veterinary Medical Science, 80(4) (4), 720 - 724, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Peripubertal exposure to the neonicotinoid pesticide dinotefuran affects dopaminergic neurons and causes hyperactivity in male mice

  Naoki Yoneda, Tadashi Takada, Tetsushi Hirano, Shogo Yanai, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Nobuhiko Hoshi

  Japanese Society of Veterinary Science, Apr. 2018, Journal of Veterinary Medical Science, 80(4) (4), 634 - 637, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Immunohistochemical study on the secretory host defense system with lysozyme and secretory phospholipase A2 throughout rat respiratory tract

  Natsumi Masuda, Youhei Mantani, Chiaki Yoshitomi, Hideto Yuasa, Miho Nishida, Masaya Arai, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Japanese Society of Veterinary Science, Feb. 2018, Journal of Veterinary Medical Science, 80(2) (2), 323 - 332, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Identification of a candidate enhancer for DMRT3 involved in spastic cerebral palsy pathogenesis

  Naoto Kubota, Toshifumi Yokoyama, Nobuhiko Hoshi, Mikita Suyama

  Elsevier B.V., Jan. 2018, Biochemical and Biophysical Research Communications, 496(1) (1), 133 - 139, English

  [Refereed]

  Scientific journal


• NOAEL-dose of a neonicotinoid pesticide, clothianidin, acutely induce anxiety-related behavior with human-audible vocalizations in male mice in a novel environment

  Tetsushi Hirano, Shogo Yanai, Tadashi Takada, Naoki Yoneda, Takuya Omotehara, Naoto Kubota, Kiichi Minami, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Jan. 2018, TOXICOLOGY LETTERS, 282, 57 - 63, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Immunohistochemical study on the distribution of β-defensin 1 and β-defensin 2 throughout the respiratory tract of healthy rats

  Natsumi Masuda, Youhei Mantani, Hideto Yuasa, Chiaki Yoshitomi, Masaya Arai, Miho Nishida, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Japanese Society of Veterinary Science, 2018, Journal of Veterinary Medical Science, 80(3) (3), 395 - 404, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mechanism of M-cell differentiation accelerated by proliferation of indigenous bacteria in rat Peyer's patches

  Hideto Yuasa, Youhei Mantani, Natsumi Masuda, Miho Nishida, Masaya Arai, Toshifumi Yokoyama, Hiroki Tsuruta, Junichi Kawano, Nobuhiko Hoshi, Hiroshi Kitagawa

  Nov. 2017, JOURNAL OF VETERINARY MEDICAL SCIENCE, 79(11) (11), 1826 - 1835, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Prenatal and early postnatal NOAEL-dose clothianidin exposure leads to a reduction of germ cells in juvenile male mice

  Shogo Yanai, Tetsushi Hirano, Takuya Omotehara, Tadashi Takada, Naoki Yoneda, Naoto Kubota, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Jul. 2017, JOURNAL OF VETERINARY MEDICAL SCIENCE, 79(7) (7), 1196 - 1203, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Contribution of the Coelomic Epithelial Cells Specific to the Left Testis in the Chicken Embryo

  Takuya Omotehara, Kiichi Minami, Youhei Mantani, Yuria Umemura, Miho Nishida, Tetsushi Hirano, Hidefumi Yoshioka, Hiroshi Kitagawa, Toshifumi Yokoyama, Nobuhiko Hoshi

  Feb. 2017, DEVELOPMENTAL DYNAMICS, 246(2) (2), 148 - 156, English

  [Refereed]

  Scientific journal


• Dose-dependent functions of fibroblast growth factor 9 regulate the fate of murine XY primordial germ cells

  Ferhat Ulu, Sung-Min Kim, Toshifumi Yokoyama, Yukiko Yamazaki

  Jan. 2017, BIOLOGY OF REPRODUCTION, 96(1) (1), 122 - 133, English

  [Refereed]

  Scientific journal


• Differential expression of Toll-like receptor-2,-4 and-9 in follicle-associated epithelium from epithelia of both follicle-associated intestinal villi and ordinary intestinal villi in rat Peyer's patches

  Hideto Yuasa, Youhei Mantani, Natsumi Masuda, Miho Nishida, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Dec. 2016, JOURNAL OF VETERINARY MEDICAL SCIENCE, 78(12) (12), 1797 - 1804, English

  [Refereed]

  Scientific journal


• Ontogenic and morphological study of gonadal formation in genetically-modified sex reversal XYPOS mice

  Yuria Umemura, Ryosuke Miyamoto, Rie Hashimoto, Kyoko Kinoshita, Takuya Omotehara, Daichi Nagahara, Tetsushi Hirano, Naoto Kubota, Kiichi Minami, Shogo Yanai, Natsumi Masuda, Hideto Yuasa, Youhei Mantani, Eiko Matsuo, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Dec. 2015, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(12) (12), 1587 - 1597, English

  [Refereed]

  Scientific journal


• Immunohistochemical analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxicity on the developmental dentate gyrus and hippocampal fimbria in fetal mice

  Yoshihiro Kobayashi, Tetsushi Hirano, Takuya Omotehara, Rie Hashimoto, Yuria Umemura, Hideto Yuasa, Natsumi Masuda, Naoto Kubota, Kiichi Minami, Shogo Yanai, Mitsuko Ishihara-Sugano, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Nov. 2015, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(11) (11), 1355 - 1361, English

  [Refereed]

  Scientific journal


• The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice

  Tetsushi Hirano, Shogo Yanai, Takuya Omotehara, Rie Hashimoto, Yuria Umemura, Naoto Kubota, Kiichi Minami, Daichi Nagahara, Eiko Matsuo, Yoshiko Aihara, Ryota Shinohara, Tomoyuki Furuyashiki, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Oct. 2015, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(10) (10), 1207 - 1215, English

  [Refereed]

  Scientific journal


• Ultrastructural study on the morphological changes in indigenous bacteria of mucous layer and chyme throughout the rat intestine

  Youhei Mantani, Eri Ito, Miho Nishida, Hideto Yuasa, Natsumi Masuda, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Sep. 2015, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(9) (9), 1121 - 1128, English

  [Refereed]

  Scientific journal


• Immunohistochemical and histoplanimetrical study on the endothelial receptor involved in transportation of minute chylomicrons into subepithelial portal blood in intestinal villi of the rat jejunum

  Ei-ichirou Takahara, Hideto Yuasa, Miho Nishida, Youhei Mantani, Kankanam Gamage Sanath Udayanga, Wang-Mei Qi, Takashi Takeuchi, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Apr. 2015, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(4) (4), 387 - 393, English

  [Refereed]

  Scientific journal


• Unpredictable Chronic Stress-Induced Reproductive Suppression Associated with the Decrease of Kisspeptin Immunoreactivity in Male Mice

  Tetsushi Hirano, Yoshihiro Kobayashi, Takuya Omotehara, Atsutoshi Tatsumi, Rie Hashimoto, Yuria Umemura, Daichi Nagahara, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Sep. 2014, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76(9) (9), 1201 - 1208, English

  [Refereed]

  Scientific journal


• Insight into the Mechanism of Reproductive Dysfunction Caused by Neonicotinoid Pesticides

  Nobuhiko Hoshi, Tetsushi Hirano, Takuya Omotehara, Junko Tokumoto, Yuria Umemura, Youhei Mantani, Takashi Tanida, Katsuhiko Warita, Yoshiaki Tabuchi, Toshifumi Yokoyama, Hiroshi Kitagawa

  Sep. 2014, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 37(9) (9), 1439 - 1443, English

  [Refereed]

  Scientific journal


• Ultrastructural and histochemical study on the Paneth cells in the rat ascending colon.

  Youhei Mantani, Miho Nishida, Hideto Yuasa, Kyouji Yamamoto, Ei-Ichirou Takahara, Takuya Omotehara, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Paneth cells (PCs) contribute to the host defense against indigenous bacteria in the small intestine. We found Paneth cell-like cells (PLCs) in the rat ascending colon, but the nature of PLCs is never clarified. Therefore, the present study aimed to clarify the cytological characteristics of PLCs and discuss their cellular differentiation. PLCs were localized in the bases of intestinal crypts, especially follicle-associated intestinal crypts in proximal colonic lymphoid tissue, but were very seldom found in the ordinary intestinal crypts of the ascending colon. PLCs possessed specific granules with highly electron-dense cores and haloes, as well as PCs in the small intestine. The secretory granules of PLCs were positive for PAS reaction, lysozyme and soluble phospholipase A2, but negative for Alcian blue staining, β-defensin-1 and -2, as well as the ones of PCs. Furthermore, intermediate cells possessing both the PLC-specific granules and the mucus granules similar to those of goblet cells (GCs) were occasionally found in the vicinity of PLCs. Intermediate cells ranged from goblet cell-like cells rich in mucus granules to PLC-like cells with few mucus granules. The cellular condensation and fragmentation were exclusively found in PLCs but never seen in intermediate cells or GCs. The PLCs, which were identified as PC, were suggested to be transformed from GCs through intermediate cells and finally to die by apoptosis in intestinal crypts of proximal colonic lymphoid tissue in the rat ascending colon.

  Aug. 2014, Anatomical record (Hoboken, N.J. : 2007), 297(8) (8), 1462 - 71, English, International magazine

  [Refereed]

  Scientific journal


• Peculiar Composition of Epithelial Cells in Follicle-Associated Intestinal Crypts of Peyer's Patches in the Rat Small Intestine

  Youhei Mantani, Hideto Yuasa, Miho Nishida, Ei-ichirou Takahara, Takuya Omotehara, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Jun. 2014, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76(6) (6), 833 - 838, English

  [Refereed]

  Scientific journal


• Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Mullerian ducts

  T. Omotehara, C. A. Smith, Y. Mantani, Y. Kobayashi, A. Tatsumi, D. Nagahara, R. Hashimoto, T. Hirano, Y. Umemura, T. Yokoyama, H. Kitagawa, N. Hoshi

  Apr. 2014, POULTRY SCIENCE, 93(4) (4), 953 - 958, English

  [Refereed]

  Scientific journal


• Short-term ethanol exposure causes imbalanced neurotrophic factor allocation in the basal forebrain cholinergic system: a novel insight into understanding the initial processes of alcohol addiction.

  Takanori Miki, Takashi Kusaka, Toshifumi Yokoyama, Ken-ichi Ohta, Shingo Suzuki, Katsuhiko Warita, Mostofa Jamal, Zhi-Yu Wang, Masaaki Ueki, Jun-Qian Liu, Tomiko Yakura, Motoki Tamai, Kazunori Sumitani, Naohisa Hosomi, Yoshiki Takeuchi

  Alcohol ingestion affects both motor and cognitive functions. One brain system that is influenced by ethanol is the basal forebrain (BF) cholinergic projection system, which projects to diverse neocortical and limbic areas. The BF is associated with memory and cognitive function. Our primary interest is the examination of how regions that receive BF cholinergic projections are influenced by short-term ethanol exposure through alterations in the mRNA levels of neurotrophic factors [nerve growth factor/TrkA, brain-derived neurotrophic factor/TrkB, and glial-derived neurotrophic factor (GDNF)/GDNF family receptor α1]. Male BALB/C mice were fed a liquid diet containing 5 % (v/v) ethanol. Pair-fed control mice were maintained on an identical liquid diet, except that the ethanol was isocalorically substituted with sucrose. Mice exhibiting signs of ethanol intoxication (stages 1-2) were used for real-time reverse transcription-polymerase chain reaction analyses. Among the BF cholinergic projection regions, decreased levels of GDNF mRNA and increased levels of TrkB mRNA were observed in the basal nucleus, and increased levels of TrkB mRNA were observed in the cerebral cortex. There were no significant alterations in the levels of expression of relevant neurotrophic factors in the septal nucleus and hippocampus. Given that neurotrophic factors function in retrograde/anterograde or autocrine/paracrine mechanisms and that BF cholinergic projection regions are neuroanatomically connected, these findings suggested that an imbalanced allocation of neurotrophic factor ligands and receptors is an initial phenomenon in alcohol addiction. The exact mechanisms underlying this phenomenon in the BF cholinergic system are unknown. However, our results provide a novel notion for the understanding of the initial processes in alcohol addiction.

  Feb. 2014, Journal of neural transmission (Vienna, Austria : 1996), 121(2) (2), 201 - 10, English, International magazine

  [Refereed]

  Scientific journal


• Fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element III in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain

  Takashi Tanida, Ken Tasaka, Eiichi Akahoshi, Mitsuko Ishihara-Sugano, Michiko Saito, Shigehisa Kawata, Megumi Danjo, Junko Tokumoto, Youhei Mantani, Daichi Nagahara, Yoshiaki Tabuchi, Toshifumi Yokoyama, Hiroshi Kitagawa, Mitsuhiro Kawata, Nobuhiko Hoshi

  Feb. 2014, JOURNAL OF APPLIED TOXICOLOGY, 34(2) (2), 117 - 126, English

  [Refereed]

  Scientific journal


• Early postnatal repeated maternal deprivation causes a transient increase in OMpg and BDNF in rat cerebellum suggesting precocious myelination

  Takanori Miki, Toshifumi Yokoyama, Takashi Kusaka, Shingo Suzuki, Ken-ichi Ohta, Katsuhiko Warita, Zhi-Yu Wang, Masaaki Ueki, Kazunori Sumitani, Frederick P. Bellinger, Motoki Tamai, Jun-Qian Liu, Tomiko Yakura, Yoshiki Takeuchi

  Jan. 2014, JOURNAL OF THE NEUROLOGICAL SCIENCES, 336(1-2) (1-2), 62 - 67, English

  [Refereed]

  Scientific journal


• Early postnatal maternal separation causes alterations in the expression of β3-adrenergic receptor in rat adipose tissue suggesting long-term influence on obesity.

  Takanori Miki, Jun-Qian Liu, Ken-ichi Ohta, Shingo Suzuki, Takashi Kusaka, Katsuhiko Warita, Toshifumi Yokoyama, Mostofa Jamal, Masaaki Ueki, Tomiko Yakura, Motoki Tamai, Kazunori Sumitani, Naohisa Hosomi, Yoshiki Takeuchi

  The effects of early postnatal maternal deprivation on the biological characteristics of the adipose tissue later in life were investigated in the present study. Sprague-Dawley rats were classified as either maternal deprivation (MD) or mother-reared control (MRC) groups. MD was achieved by separating the rat pups from their mothers for 3h each day during the 10-15 postnatal days. mRNA levels of mitochondrial uncoupling protein 1 (UCP-1), β3-adrenergic receptor (β3-AR), and prohibitin (PHB) in the brown and white adipose tissue were determined using real-time RT-PCR analysis. UCP-1, which is mediated through β3-AR, is closely involved in the energy metabolism and expenditure. PHB is highly expressed in the proliferating tissues/cells. At 10 weeks of age, the body weight of the MRC and MD rats was similar. However, the levels of the key molecules in the adipose tissue were substantially altered. There was a significant increase in the expression of PHB mRNA in the white adipose tissue, while the β3-AR mRNA expression decreased significantly, and the UCP-1 mRNA expression remained unchanged in the brown adipose tissue. Given that these molecules influence the mitochondrial metabolism, our study indicates that early postnatal maternal deprivation can influence the fate of adipose tissue proliferation, presumably leading to obesity later in life.

  Dec. 2013, Biochemical and biophysical research communications, 442(1-2) (1-2), 68 - 71, English, International magazine

  [Refereed]

  Scientific journal


• Ultrastructural Demonstration of the Absorption and Transportation of Minute Chylomicrons by Subepithelial Blood Capillaries in Rat Jejunal Villi

  Ei-ichirou Takahara, Youhei Mantani, Kankanam Gamage Sanath Udayanga, Wang-Mei Qi, Takashi Tanida, Takashi Takeuchi, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Dec. 2013, JOURNAL OF VETERINARY MEDICAL SCIENCE, 75(12) (12), 1563 - 1569, English

  [Refereed]

  Scientific journal


• Histoplanimetrical Study on the Relationship between Invasion of Indigenous Bacteria into Intestinal Crypts and Proliferation of Epithelial Cells in Rat Ascending Colon

  Youhei Mantani, Ei-ichirou Takahara, Takashi Takeuchi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Jul. 2013, JOURNAL OF VETERINARY MEDICAL SCIENCE, 75(7) (7), 939 - 947, English

  [Refereed]

  Scientific journal


• Histoplanimetrical study on the relationship between invasion of indigenous bacteria into intestinal crypts and proliferation of epithelial cells in rat ascending colon.

  MANTANI YOUHEI, TAKAHARA EI-ICHIRO, TAKEUCHI TAKASHI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  Jul. 2013, The Journal of Veterinary Medical Science, 75(5) (5), 939 - 947, English

  [Refereed]

  Scientific journal


• Effects of Exposure to Clothianidin on the Reproductive System of Male Quails

  Junko Tokumoto, Megumi Danjo, Yoshihiro Kobayashi, Kyoko Kinoshita, Takuya Omotehara, Atsutoshi Tatsumi, Mineo Hashiguchi, Tsuneo Sekijima, Hiroshi Kamisoyama, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Jun. 2013, JOURNAL OF VETERINARY MEDICAL SCIENCE, 75(6) (6), 755 - 760, English

  [Refereed]

  Scientific journal


• Immunohistochemical analysis of steroidogenic acute regulatory protein (StAR) and StAR-binding protein (SBP) expressions in the testes of mice during fetal development

  Warita K, Mitsuhashi T, Fukui S, Ohta K, Suzuki S, Miki T, Takeuchi Y, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, Sugawara T, HOSHI NOBUHIKO

  May 2013, Reproductive Biology, 13(1) (1), 92 - 95, English

  [Refereed][Invited]

  Scientific journal


• Immunohistochemical analysis of steroidogenic acute regulatory protein (StAR) and StAR-binding protein (SBP) expressions in the testes of mice during fetal development

  Katsuhiko Warita, Tomoko Mitsuhashi, Shiho Fukui, Ken-ichi Ohta, Shingo Suzuki, Takanori Miki, Yoshiki Takeuchi, Toshifumi Yokoyama, Hiroshi Kitagawa, Teruo Sugawara, Nobuhiko Hoshi

  Mar. 2013, REPRODUCTIVE BIOLOGY, 13(1) (1), 92 - 95, English

  [Refereed]

  Scientific journal


• Differential effects of neonatal maternal separation on the expression of neurotrophic factors in rat brain. II: Regional differences in the cerebellum versus the cerebral cortex.

  Takanori Miki, Kyoung-Youl Lee, Toshifumi Yokoyama, Jun-Qian Liu, Takashi Kusaka, Shingo Suzuki, Ken-ichi Ohta, Katsuhiko Warita, Mostofa Jamal, Masaaki Ueki, Tomiko Yakura, Naohisa Hosomi, Yoshiki Takeuchi

  This study was conducted in order to examine the effects of early postnatal maternal separation stress on the age-dependent fluctuations in the expression levels of neurotrophic factor ligands and receptors in the developing cerebellum. Wistar rats were separated from their mothers for 3 h each day during postnatal days (PND) 10 to 15. The expression level of mRNA for brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor B (TrkB), insulin-like growth factor-1 (IGF-1), and type-1 IGF receptor (IGF-1R) were evaluated in the cerebellum on PND16, 20, 30, and 60 with real-time RT-PCR. The mRNA levels of cerebellar BDNF in maternally separated rats were increased on PND16, while the other variables showed no significant alterations at any of the time points examined. However, the effects of an identical maternal separation on the cerebral cortex were previously reported to be completely different. These results indicate regional differences in the responses of neurotrophic factor ligands/receptors between the cerebellum and cerebral cortex. Given that neurotrophic factors play important roles in brain development, alterations in these factors may interrupt normal brain development and ultimately, lead to functional disruptions.

  2013, Okajimas folia anatomica Japonica, 90(3) (3), 53 - 8, English, Domestic magazine

  Scientific journal


• Persorption of IgG-Fc-Coated Particulates from Intestinal Lumen into Portal Blood via Vinous Columnar Epithelial Cells in Rat Small Intestine

  Midori Yuji, Masato Fujimoto, Wang-Mei Qi, Ei-ichirou Takahara, Youhei Mantani, Kankanam Gamage Sanath Udayanga, Takashi Takeuchi, Katsuhiko Warita, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Nov. 2012, JOURNAL OF VETERINARY MEDICAL SCIENCE, 74(11) (11), 1447 - 1452, English

  [Refereed]

  Scientific journal


• Immunohistochemical Detection of Toll-Like Receptor-2,-4 and-9 in Exocrine Glands Associated with Rat Alimentary Tract

  Youhei Mantani, Yuh Yokoo, Aosa Kamezaki, Kankanam Gamage Sanath Udayanga, Ei-ichirou Takahara, Takashi Takeuchi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Nov. 2012, JOURNAL OF VETERINARY MEDICAL SCIENCE, 74(11) (11), 1429 - 1438, English

  [Refereed]

  Scientific journal


• Alteration in the Apoptosis Process of Rat Esophageal Epithelium with Hyperproliferation of Indigenous Bacteria under a Physiological Condition

  Kankanam Gamage Sanath Udayanga, Kyoji Yamamoto, Hidenori Miyata, Yuh Yokoo, Youhei Mantani, Ei-ichrou Takahara, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  May 2012, JOURNAL OF VETERINARY MEDICAL SCIENCE, 74(5) (5), 597 - 605, English

  [Refereed]

  Scientific journal


• Neonatal repetitive maternal separation causes long-lasting alterations in various neurotrophic factor expression in the cerebral cortex of rats

  Kyoung-Youl Lee, Takanori Miki, Toshifumi Yokoyama, Masaaki Ueki, Katsuhiko Warita, Shingo Suzuki, Ken-Ichi Ohta, Zhi-Yu Wang, Mostofa Jamal, Tomiko Yakura, Jun-Qian Liu, Naohisa Hosomi, Yoshiki Takeuchi

  Apr. 2012, LIFE SCIENCES, 90(15-16) (15-16), 578 - 584, English

  [Refereed]

  Scientific journal


• Genetic Variation in AhR Gene Related to Dioxin Sensitivity in the Japanese Field Mouse, Apodemus speciosus

  Hiroko Ishiniwa, Kazuhiro Sogawa, Ken-ichi Yasumoto, Nobuhiko Hoshi, Toshifumi Yokoyama, Ken Tasaka, Tsuneo Sekijim

  InTech, Feb. 2012, Immunosuppression - Role in Health and Diseases

  [Refereed]


• Site differences of Toll-like receptor expression in the mucous epithelium of rat small intestine

  Youhei Mantani, Aosa Kamezaki, Kankanam Gamage Sanath Udayanga, Ei-ichirou Takahara, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Oct. 2011, HISTOLOGY AND HISTOPATHOLOGY, 26(10) (10), 1295 - 1303, English

  [Refereed]

  Scientific journal


• A high cholesterol diet given to apolipoprotein E-knockout mice has a differential effect on the various neurotrophin systems in the hippocampus

  Zhi-Yu Wang, Takanori Miki, Yan Ding, Shi-Jie Wang, Yu-Huan Gao, Xiao-Ling Wang, Yu-Hua Wang, Toshifumi Yokoyama, Katsuhiko Warita, Ken-ichi Ohta, Shingo Suzuki, Taira Ohnishi, Takashi Obama, Kuldip S. Bedi, Yoshiki Takeuchi, Bao-En Shan

  Sep. 2011, METABOLIC BRAIN DISEASE, 26(3) (3), 185 - 194, English

  [Refereed]

  Scientific journal


• Immunohistochemical and Histoplanimetrical Study on the Spatial Relationship between the Settlement of Indigenous Bacteria and the Secretion of Bactericidal Peptides in Rat Alimentary Tract

  Yuh Yokoo, Hidenori Miyata, Kankanam Gamage Sanath Udayanga, Wang-Mei Qi, Ei-ichirou Takahara, Youhei Mantani, Toshifumi Yokoyama, Junichi Kawano, Nobuhiko Hoshi, Hiroshi Kitagawa

  Aug. 2011, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73(8) (8), 1043 - 1050, English

  [Refereed]

  Scientific journal


• Immunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological condition

  K. G. S. Udayanga, H. Miyata, Y. Yokoo, W. -M. Qi, E. Takahara, Y. Mantani, T. Yokoyama, N. Hoshi, H. Kitagawa

  Jul. 2011, HISTOLOGY AND HISTOPATHOLOGY, 26(7) (7), 811 - 820, English

  [Refereed]

  Scientific journal


• Immunohistochemical Detection of Phosphatidylserine and Thrombospondin on Denucleating Erythroblasts in Rat Bone Marrow

  Toshifumi Yokoyama, Takahiko Etoh, Kankanam Gamage Sanath Udayanga, Eiichiro Takahara, Wang-Mei Qi, Katsuhiko Warita, Yoshiki Matsumoto, Takanori Miki, Yoshiki Takeuchi, Nobuhiko Hoshi, Hiroshi Kitagawa

  Jul. 2011, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73(7) (7), 949 - 952, English

  [Refereed]

  Scientific journal


• Immunohistochemical study on the secretory host defense system of bactericidal peptides in rat digestive organs

  Yuh Yokoo, Hidenori Miyata, Kankanam Gamage Sanath Udayanga, Wang-Me Qi, Ei-ichioru Takahara, Toshifumi Yokoyama, Junichi Kawano, Nobuhiko Hoshi, Hiroshi Kitagawa

  Feb. 2011, Journal of Veterinary Medical Science, 73(2) (2), 217 - 225, English

  [Refereed]

  Scientific journal


• Lectin Histochemical Detection of Special Sugars on the Mucosal Surfaces of the Rat Alimentary Tract

  Kenkichi Yamamoto, Wang-Mei Qi, Yuh Yokoo, Hidenori Miyata, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Sep. 2010, JOURNAL OF VETERINARY MEDICAL SCIENCE, 72(9) (9), 1119 - 1127, English

  [Refereed]

  Scientific journal


• Direct effects of diethylstilbestrol on the gene expression of the cholesterol side-chain cleavage enzyme (P450scc) in testicular Leydig cells

  Katsuhiko Warita, Tomoko Mitsuhashi, Teruo Sugawara, Yoshiaki Tabuchi, Takashi Tanida, Zhi-Yu Wang, Yoshiki Matsumoto, Toshifumi Yokoyama, Hiroshi Kitagawa, Takanori Miki, Yoshiki Takeuchi, Nobuhiko Hoshi

  Aug. 2010, LIFE SCIENCES, 87(9-10) (9-10), 281 - 285, English

  [Refereed]

  Scientific journal


• Epigenetic abnormality of SRY gene in the adult XY female with pericentric inversion of the Y chromosome

  Tomoko Mitsuhashi, Katsuhiko Warita, Teruo Sugawara, Yoshiaki Tabuchi, Ichiro Takasaki, Takashi Kondo, Fumio Hayashi, Zhi-Yu Wang, Yoshiki Matsumoto, Takanori Miki, Yoshiki Takeuchi, Yasuhiko Ebina, Hideto Yamada, Noriaki Sakuragi, Toshifumi Yokoyama, Takashi Nanmori, Hiroshi Kitagawa, Jeffrey A. Kant, Nobuhiko Hoshi

  Jun. 2010, CONGENITAL ANOMALIES, 50(2) (2), 85 - 94, English

  [Refereed]

  Scientific journal


• Global gene profiling and comprehensive bioinformatics analysis of a 46,XY female with pericentric inversion of the Y chromosome

  Tomoko Mitsuhashi, Katsuhiko Warita, Yoshiaki Tabuchi, Ichiro Takasaki, Takashi Kondo, Teruo Sugawara, Fumio Hayashi, Zhi-Yu Wang, Yoshiki Matsumoto, Takanori Miki, Yoshiki Takeuchi, Yasuhiko Ebina, Hideto Yamada, Noriaki Sakuragi, Toshifumi Yokoyama, Takashi Nanmori, Hiroshi Kitagawa, Jeffrey A. Kant, Nobuhiko Hoshi

  Mar. 2010, CONGENITAL ANOMALIES, 50(1) (1), 40 - 51, English

  [Refereed]

  Scientific journal


• SHORT-TERM EXPOSURE TO ETHANOL CAUSES A DIFFERENTIAL RESPONSE BETWEEN NERVE GROWTH FACTOR AND BRAIN-DERIVED NEUROTROPHIC FACTOR LIGAND/RECEPTOR SYSTEMS IN THE MOUSE CEREBELLUM

  Z. Y. Wang, T. Miki, K. Y. Lee, T. Yokoyama, T. Kusaka, K. Sumitani, K. Warita, Y. Matsumoto, T. Yakura, N. Hosomi, K. Ameno, K. S. Bedi, Y. Takeuchi

  Jan. 2010, NEUROSCIENCE, 165(2) (2), 485 - 491, English

  [Refereed]

  Scientific journal


• GABA expression in c-Fos immunoreactive neurons of the rat periaqueductal gray induced by electroacupuncture at the point of Zusanli

  FUSUMADA Kazutoshi, YOKOYAMA Toshifumi, MIKI Takanori, MATSUMOTO Yoshiki, WARITA Katsuhiko, WANG Zhiyu, YAKURA Tomiko, LIU JQ, TAKEUCHI Yoshiki

  2010, Current Neurobiology, 1, pp.10-13(1) (1), 10 - 13, English

  [Refereed]

  Scientific journal


• Effectts of short-term ethanol ingestion on the expression of neurotrophins and their receptors: No changes in the expression of the glial-derived neurotrophic factor in the mouse hippocampus

  UENO Masaki, MIKI Takanori, WANG Zhiyu, YOKOYAMA Toshifumi, LEE Kyong-Youl, KUSAKA Takashi, WARITA Katsuhiko, SUMITANI Kazunori, MATSUMOTO Yoshiki, YAKURA Tomiko, LIU JQ, BEDI Kuldip S, TAKEUCHI Yoshiki

  2010, Current Neurobiology, 1, pp.40-45(1) (1), 40 - 45, English

  [Refereed]

  Scientific journal


• When does the sex ratio of offspring of the paternal 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure decrease: In the spermatozoa stage or at fertilization?

  Kana Ishihara, Seiichiroh Ohsako, Ken Tasaka, Hiroshi Harayama, Masashi Miyake, Katsuhiko Warita, Takashi Tanida, Tomoko Mitsuhashi, Takashi Nanmori, Yoshiaki Tabuchi, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Jan. 2010, REPRODUCTIVE TOXICOLOGY, 29(1) (1), 68 - 73, English

  [Refereed]

  Scientific journal


• Fetal and neonatal exposure to three typical environmental chemicals with different mechanisms of action: Mixed exposure to phenol, phthalate, and dioxin cancels the effects of sole exposure on mouse midbrain dopaminergic nuclei

  Takashi Tanida, Katsuhiko Warita, Kana Ishihara, Shiho Fukui, Tomoko Mitsuhashi, Teruo Sugawara, Yoshiaki Tabuchi, Takashi Nanmori, Wang-Mei Qi, Tetsurou Inamoto, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Aug. 2009, TOXICOLOGY LETTERS, 189(1) (1), 40 - 47, English

  [Refereed]

  Scientific journal


• Morphological Analyses of Sex Differences and Age-Related Changes in C3H Mouse Midbrain

  Takashi Tanida, Katsuhiko Warita, Tomoko Mitsuhashi, Kana Ishihara, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Jul. 2009, JOURNAL OF VETERINARY MEDICAL SCIENCE, 71(7) (7), 855 - 863, English

  [Refereed]

  Scientific journal


• Histoplanimetrical Study on the Relationship between Cellular Kinetics of Epithelial Cells and Proliferation of Indigenous Bacteria in the Rat Colon

  Wang-Mei Qi, Kenkichi Yamamoto, Yuh Yokoo, Hidenori Miyata, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Jun. 2009, JOURNAL OF VETERINARY MEDICAL SCIENCE, 71(6) (6), 745 - 752, English

  [Refereed]

  Scientific journal


• Anterografe synaptic transport of neuronal tracer enzyme (WGA-HRP): Futher studies with Rab3A-siRNA in rats.

  TAKEUCHI YOSHIKI, MATSUMOTO YOSHIKI, MIKI TAKANORI, YOKOYAMA TOSHIFUMI, WARITA KATSUHIKO, WANG ZHI-YU, UENO TAKASHI, YAKURA TOMIKO, FUJITA MAMORU

  May 2009, Journal of Biomedical Research, 20(3) (3), 149 - 154, English

  [Refereed]

  Scientific journal


• Histoplanimetrical Study on the Spatial Relationship of Distribution of Indigenous Bacteria with Mucosal Lymphatic Follicles in Alimentary Tract of Rat

  Kenkichi Yamamoto, Wang-Mei Qi, Yuh Yokoo, Hidenori Miyata, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  May 2009, JOURNAL OF VETERINARY MEDICAL SCIENCE, 71(5) (5), 621 - 630, English

  [Refereed]

  Scientific journal


• Histoplanimetrical Study on the Relationship between the Cell Kinetics of Villous Columnar Epithelial Cells and the Proliferation of Indigenous Bacteria in Rat Small Intestine

  Wang-Mei Qi, Kenkichi Yamamoto, Yuh Yokoo, Hidenori Miyata, Tetsurou Inamoto, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Apr. 2009, JOURNAL OF VETERINARY MEDICAL SCIENCE, 71(4) (4), 463 - 470, English

  [Refereed]

  Scientific journal


• Anterograde synaptic transport of neuronal tracer enzyme (WGA-HRP): approach of Rab3A-siRNA injection into the vagus nerve in the rat

  Yoshiki Takeuchi, Yoshiki Matsumoto, Takanori Miki, Toshifumi Yokoyama, Katsuhiko Warita, Zhi-Yu Wang, Tomiko Yakura, Mamoru Fujita

  Elsevier BV, Jan. 2009, Neuroscience Research, 65, S80 - S81

  [Refereed]

  Scientific journal


• An Immunohistochemical Detection of Actin and Myosin in the Indigenous Bacteria-Adhering Sites of Microvillous Columnar Epithelial Cells in Peyer's Patches and Intestinal Villi in the Rat Jejunoileum

  Tetsurou Inamoto, Makiko Namba, Wang-Mei Qi, Kenkichi Yamamoto, Yuh Yokoo, Hidenori Miyata, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Nov. 2008, JOURNAL OF VETERINARY MEDICAL SCIENCE, 70(11) (11), 1153 - 1158, English

  [Refereed]

  Scientific journal


• Ethanol neurotoxicity and dentate gyrus development.

  Takanori Miki, Toshifumi Yokoyama, Kazunori Sumitani, Takashi Kusaka, Katsuhiko Warita, Yoshiki Matsumoto, Zhi-Yu Wang, Peter A Wilce, Kuldip S Bedi, Susumu Itoh, Yoshiki Takeuchi

  Maternal alcohol ingestion during pregnancy adversely affects the developing fetus, often leading to fetal alcohol syndrome (FAS). One of the most severe consequences of FAS is brain damage that is manifested as cognitive, learning, and behavioral deficits. The hippocampus plays a crucial role in such abilities; it is also known as one of the brain regions most vulnerable to ethanol-induced neurotoxicity. Our recent studies using morphometric techniques have further shown that ethanol neurotoxicity appears to affect the development of the dentate gyrus in a region-specific manner; it was found that early postnatal ethanol exposure causes a transitory deficit in the hilus volume of the dentate gyrus. It is strongly speculated that such structural modifications, even transitory ones, appear to result in developmental abnormalities in the brain circuitry and lead to the learning disabilities observed in FAS children. Based on reports on possible factors deciding ethanol neurotoxicity to the brain, we review developmental neurotoxicity to the dentate gyrus of the hippocampal formation.

  3, Sep. 2008, Congenital anomalies, 48(3) (3), 110 - 7, English, International magazine

  [Refereed]

  Scientific journal


• Ultrastructural study on the epithelial responses against attachment of indigenous bacteria to epithelial membranes in Peyer's patches of rat small intestine

  Tetsurou Inamoto, Yuko Kawata, Wang-Mei Qi, Kenkichi Yamamoto, Katsuhiko Warita, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Mar. 2008, JOURNAL OF VETERINARY MEDICAL SCIENCE, 70(3) (3), 235 - 241, English

  [Refereed]

  Scientific journal


• Dietary protein modulates circadian changes in core body temperature and metabolic rate in rats

  Ippei Yamaoka, Mitsuo Nakayama, Takanori Miki, Toshifumi Yokoyama, Yoshiki Takeuchi

  Feb. 2008, JOURNAL OF PHYSIOLOGICAL SCIENCES, 58(1) (1), 75 - 81, English

  Scientific journal


• Activin A and Equine Chorionic Gonadotropin Recover Reproductive Dysfunction Induced by Neonatal Exposure to an Estrogenic Endocrine Disruptor in Adult Male Mice1

  Katsuhiko Warita, Kazutaka Okamoto, Ken-ichiro Mutoh, Yoshihisa Hasegawa, Zhan-Peng Yue, Toshifumi Yokoyama, Yoshiki Matsumoto, Takanori Miki, Yoshiki Takeuchi, Hiroshi Kitagawa, Teruo Sugawara, Nobuhiko Hoshi

  Oxford University Press (OUP), Jan. 2008, Biology of Reproduction, 78(1) (1), 59 - 67

  [Refereed]

  Scientific journal


• Early postnatal ethanol exposure induces fluctuation in the expression of BDNF mRNA in the developing rat hippocampus

  Takanori Miki, Hiromi Kuma, Toshifumi Yokoyama, Kazunori Sumitani, Yoshiki Matsumoto, Takashi Kusaka, Katsuhiko Warita, Hi-Yu Wang, Naohisa Hosomi, Tomohiro Imagawa, Kuldip S. Bedi, Susumu Itoh, Yu Nakamura, Yoshiki Takeuchi

  2008, ACTA NEUROBIOLOGIAE EXPERIMENTALIS, 68(4) (4), 484 - 493, English

  [Refereed]

  Scientific journal


• Immunohistochemical study on the delayed progression of epithelial apoptosis in follicle-associated epithelium of rat Peyer's patch

  Sachiko Onishi, Hidenori Miyata, Tetsurou Inamoto, Wang-Mei Qi, Kenkichi Yamamoto, Toshifumi Yokoyama, Katsuhiko Warita, Nobuhiko Hoshi, Hiroshi Kitagawa

  Nov. 2007, JOURNAL OF VETERINARY MEDICAL SCIENCE, 69(11) (11), 1123 - 1129, English

  [Refereed]

  Scientific journal


• The effect of prenatal X-irradiation on the developing cerebral cortex of rats. II: A quantitative assessment of glial cells in the somatosensory cortex

  Takanori Miki, Toshifumi Yokoyama, Kazunori Sumitani, Zhi-Yu Wang, Weiwen Yang, Takashi Kusaka, Yoshiki Matsumoto, Katsuhiko Warita, Nam-Seob Lee, Yoshihiro Fukui, Yoshiki Takeuchi

  Aug. 2007, INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 25(5) (5), 293 - 297, English

  [Refereed]

  Scientific journal


• C-Fos expression in the periaqueductal gray is induced by electroacupuncture in the rat, with possible reference to GABAergic neurons

  Kazutoshi Fusumada, Toshifumi Yokoyama, Takanori Miki, Zhi-Yu Wang, Weiwen Yang, Nam-Seob Lee, Yoko Endo, Yoshiki Takeuchi

  May 2007, Okajimas Folia Anatomica Japonica, 84(1) (1), 1 - 10, English

  [Refereed]

  Scientific journal


• Ultrastructural study on the differentiation and the fate of M cells in follicle-associated epithelium of rat peyer's patch

  Sachiko Onishi, Toshifumi Yokoyama, Keigi Chin, Midori Yuji, Tetsurou Inamoto, Wang-Mei Qi, Katsuhiko Warita, Nobuhiko Hoshi, Hiroshi Kitagawa

  May 2007, JOURNAL OF VETERINARY MEDICAL SCIENCE, 69(5) (5), 501 - 508, English

  [Refereed]

  Scientific journal


• Persorption mechanisms of luminal antigenic particulates via apoptotic epithelial cells of intestinal villi into systemic blood circulation in orally immunized rats

  Midori Yuji, Masato Fujimoto, Hidenori Miyata, Tetsurou Inamoto, Wang-Mei Qi, Kenkichi Yamamoto, Katsuhiko Warita, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Apr. 2007, JOURNAL OF VETERINARY MEDICAL SCIENCE, 69(4) (4), 339 - 346, English

  [Refereed]

  Scientific journal


• Special sugar expression on apoptotic epithelial cells of Peyer's patches and intestinal villi in rat small intestine

  Keigi Chin, Sachiko Onishi, Midori Yuji, Tetsurou Inamoto, Wang-Mei Qi, Kenkichi Yamamoto, Katsuhiko Warita, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Feb. 2007, JOURNAL OF VETERINARY MEDICAL SCIENCE, 69(2) (2), 193 - 199, English

  [Refereed]

  Scientific journal


• Age-related changes in growth hormone-immunoreactive cells in the anterior pituitary gland of Jcl: Wistar-TgN (ARGHGEN) 1Nts rats (Mini rats)

  Matsumoto Yoshiki, Tsukamoto Yasuhiro, Miki Takanori, Ogawa Kazushige, Lee Kyoung-Youl, Yokoyama Toshifumi, Satriotomo Irawan, Li Hong-Peng, Gu He, Wang Zhi-Yu, Karasawa Shigeru, Ueda Susumu, Sasaki Fumihiko, Takeuchi Yoshiki

  Wiley, Dec. 2006, Congenital Anomalies, 46(4) (4), 188 - 193


• Persorption of luminal antigenic molecule and its specific antibody via apoptotic epithelial cells of intestinal villi and Peyer's patches into peripheral blood in rats

  Midori Yuji, Masahito Tsubata, Keigi Chin, Sachiko Onishi, Tetsurou Inamoto, Wang-Mei Qi, Katsuhiko Warita, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Dec. 2006, JOURNAL OF VETERINARY MEDICAL SCIENCE, 68(12) (12), 1297 - 1305, English

  [Refereed]

  Scientific journal


• Oligodendrocyte myelin glycoprotein (OMgp) in rat hippocampus is depleted by chronic ethanol consumption

  Hanayo Okamoto, Takanori Miki, Kyoung-Youl Lee, Toshifumi Yokoyama, Hiromi Kuma, Zhi-Yu Wang, He Gu, Hong-Peng Li, Yoshiki Matsumoto, Satriotomo Irawan, Kuldip S. Bedi, Yu Nakamura, Yoshiki Takeuchi

  Oct. 2006, NEUROSCIENCE LETTERS, 406(1-2) (1-2), 76 - 80, English

  Scientific journal


• Differentiation of epithelial cells to M cells in response to bacterial colonization on the follicle-associated epithelium of Peyer's patch in rat small intestine

  Keigi Chin, Sachiko Onishi, Midori Yuji, Tetsurou Inamoto, Wang-Mei Qi, Katsuhiko Warita, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

  Oct. 2006, JOURNAL OF VETERINARY MEDICAL SCIENCE, 68(10) (10), 1023 - 1028, English

  [Refereed]

  Scientific journal


• Regional differences in vulnerability of the cerebellar foliations of rats exposed to neonatal X-irradiation

  HP Li, T Miki, T Yokoyama, KY Lee, H Gu, Y Matsumoto, ZY Wang, H Kawano, Y Takeuchi

  Jul. 2006, NEUROSCIENCE LETTERS, 402(1-2) (1-2), 86 - 91, English

  Scientific journal


• Changes of parvalburnin immunoreactive neurons and GFAP immunoreactive astrocytes in the rat lateral geniculate nucleus following monocular enucleation

  D Gonzalez, Satriotomo, I, T Miki, KY Lee, T Yokoyama, T Touge, Y Matsumoto, A Li, S Kuriyama, Y Takeuchi

  Mar. 2006, NEUROSCIENCE LETTERS, 395(2) (2), 149 - 154, English

  Scientific journal


• Effects of Chronic Ethanol Administration on the Expression Levels of Neurotrophic Factors in the Rat Hippocampus

  OKAMOTO Hanayo, FUSUMADA Kazutoshi, IMAGAWA Tomohiro, WANG Zhi-Yu, NAKAMURA Yu, TAKEUCHI Yoshiki, MIKI Takanori, LEE Kyoung-Youl, YOKOYAMA Toshifumi, KUMA Hiromi, GU He, LI Hong-Peng, MATSUMOTO Yoshiki, YAMAOKA Ippei

  Chronic ethanol consumption has adverse effects on the central nervous system. Hippocampus is one of the target sites of ethanol neurotoxicity. Hippocampal damage is known to result in impairment of learning and memory. This study was aimed to determine whether chronic ethanol consumption could alter the expression levels of brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF) mRNAs in the hippocampus. Male Wistar rats were given unrestricted access to a liquid diet containing 5% (v/v) ethanol as the sole fluid source for 19 weeks beginning at 10 weeks of age. The expression levels of BDNF and GDNF mRNAs in the hippocampus were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. The present study revealed that chronic ethanol consumption did not result in significant changes in the expression levels of BDNF and GDNF mRNAs. Our present results showed no significant alteration in the expression of these neurotrophic factors; these results will lead to further studies to examine the possible alterations in the gene expression of various neurotrophins that are related to hippocampal functions including learning and memory.

  Editorial Board of Okajimas Folia Anatomica Japonica, 2006, Okajimas Folia Anatomica Japonica, 83(1) (1), 1 - 6, English


• Application of the physical disector to the central nervous system: Estimation of the total number of neurons in subdivisions of the rat hippocampus

  Takanori Miki, Irawan Satriotomo, Hong-Peng Li, Yoshiki Matsumoto, He Gu, Toshifumi Yokoyama, Kyoung-Youl Lee, Kuldip S. Bedi, Yoshiki Takeuchi

  Sep. 2005, Anatomical Science International, 80(3) (3), 153 - 162, English


• Effects of Monocular Enucleation on Calbindin-D 28k and c-Fos Expression in the Lateral Geniculate Nucleus in Rats

  GONZALEZ Daniel, TAKEUCHI Yoshiki, SATRIOTOMO Irawan, MIKI Takanori, LEE Kyoung-Youl, YOKOYAMA Toshifumi, TOUGE Tetsuo, MATSUMOTO Yoshiki, LI Hong-Peng, KURIYAMA Shigeki

  The present study was undertaken to evaluate the effects of monocular enucleation on the calbindin-D 28k (CB) and c-Fos immunoreactive (IR) neurons in the lateral geniculate nucleus (LGN) complex of adult rats. The enucleation resulted in neuronal degeneration and decrease of neurons in the LGN complex. Our study demonstrated a decrease of CB-IR neuronal density on the contralateral side of the ventral (vLGN) and dorsal LGN (dLGN) until 12 weeks post-enucleation (PE). On the ipsilateral side, CB-IR neuronal density in the dLGN and vLGN showed significant and continuous decrease until 48 and 12 weeks PE, respectively. In an additional experiment, c-Fos-IR neurons were increased at 2 days PE in the vLGN with ipsilateral predominance. At 7 days, c-Fos-IR neurons on the ipsilateral vLGN were still higher than those of pre-enucleated rats. The present results suggest that monocular enucleation affects the expression of the CB and c-Fos in the LGN complex. It is indicated that these may play an important role in the neuronal degeneration and neuroplasticity of the subvisual system in rats.

  Editorial Board of Okajimas Folia Anatomica Japonica, 2005, Okajimas Folia Anatomica Japonica, 82(1) (1), 9 - 18, English


• Early maternal deprivation induces alterations in brain-derived neurotrophic factor expression in the developing rat hippocampus

  H Kuma, T Miki, Y Matsumoto, H Gu, HP Li, T Kusaka, Satriotomo, I, H Okamoto, T Yokoyama, KS Bedi, S Onishi, H Suwaki, Y Takeuchi

  Nov. 2004, NEUROSCIENCE LETTERS, 372(1-2) (1-2), 68 - 73, English

  Scientific journal


• Postnatal apoptosis, development, and sex difference in the lateral septum of rats

  S Tsukahara, K Inami, F Maekawa, M Kakeyama, T Yokoyama, M Yuji, H Kitagawa, Y Kannan, K Yamanouchi

  Jul. 2004, JOURNAL OF COMPARATIVE NEUROLOGY, 475(2) (2), 177 - 187, English

  [Refereed]

  Scientific journal


• Persorption of bovine lactoferrin from the intestinal lumen into the systemic circulation via the portal vein and the mesenteric lymphatics in growing pigs

  H Kitagawa, Y Yoshizawa, T Yokoyama, T Takeuchi, MJR Talukder, H Shimizu, K Ando, E Harada

  May 2003, JOURNAL OF VETERINARY MEDICAL SCIENCE, 65(5) (5), 567 - 572, English

  Scientific journal


• Migration of erythroblastic islands toward the sinusoid as erythroid maturation proceeds in rat bone marrow

  T Yokoyama, T Etoh, H Kitagawa, S Tsukahara, Y Kannan

  Apr. 2003, JOURNAL OF VETERINARY MEDICAL SCIENCE, 65(4) (4), 449 - 452, English

  [Refereed]

  Scientific journal


• The cellular differentiation of M cells from crypt undifferentiated epithelial cells into microvillous epithelial cells in follicle-associated epithelia of chicken cecal tonsils

  Hiroshi Kitagawa, Masashi Hosokawa, Takashi Takeuchi, Toshifumi Yokoyama, Tomohiro Imagawa, Masato Uehara

  Maruzen Co. Ltd., 2003, Journal of Veterinary Medical Science, 65(2) (2), 171 - 178, English

  [Refereed]

  Scientific journal


• No apoptotic cell death of erythroid cells of erythroblastic islands in bone marrow of healthy rats.

  YOKOYAMA Toshifumi, ETOH Takao, KITAGAWA Hiroshi, TSUKAHARA Shinji, KANNAN Yasuyuki

  2003, The Journal of Veterinary Medical Science, 64(10) (10), 913 - 919, English

  [Refereed]

  Scientific journal

• 獣医組織学 改訂第9版-第4章 血液及び骨髄（骨髄）

  学窓社, Mar. 2023


• 獣医組織学 改訂第9版-第19章 家禽の組織学（1.血液，2.骨髄）

  学窓社, Mar. 2023


• 獣医組織学 改訂第8版-第19章 家禽の組織学（1.血液，2.骨髄）

  学窓社, Mar. 2020


• 獣医組織学 改訂第8版-第4章 血液及び骨髄（骨髄）

  学窓社, Mar. 2020


• 獣医組織学 改訂第7版-第4章 血液及び骨髄（骨髄）

  YOKOYAMA TOSHIFUMI

  Others, 学窓社, Mar. 2017, Japanese

  Textbook


• 獣医組織学 改訂第6版-第4章 血液及び骨髄（骨髄）

  YOKOYAMA TOSHIFUMI

  Others, 学窓社, Mar. 2014, Japanese

  Textbook

• Histological analysis of reproductive tract formation disorders in B6N-XYPOS mice

  Honoka Kawai, Toshifumi Yokoyama, Chihiro Inuzuka, Sachika Kuramoto, Youhei Mantani, Takanori Miki, Nobuhiko Hoshi

  APPW2025, English

  Poster presentation


• マウスにおけるフェニルピラゾール系農薬フィプロニルの胎子・新生子期曝露の次世代影響

  吉本彩乃, 伊藤槙子, 布引沙利香, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星信彦

  日本内分泌撹乱物質学会第26回研究発表会, Dec. 2024, Japanese

  Oral presentation


• ネオニコチノイド系農薬クロチアニジンの無毒性量（NOAEL）曝露による母性行動への継世代影響

  布引沙利香, 吉本彩乃, 伊藤槙子, 森下理奈子, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星信彦

  日本内分泌撹乱物質学会第26回研究発表会, Dec. 2024, Japanese

  Oral presentation


• クロチアニジン父性曝露による精子microRNAの発現変動と神経行動学的な次世代影響

  伊藤槙子, 布引沙利香, 吉本彩乃, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星信彦

  日本内分泌撹乱物質学会第26回研究発表会, Dec. 2024, Japanese

  Oral presentation


• 「Developmental neurotoxicity of fetal and neonatal exposure to the phenylpyrazole pesticide fipronil in mice

  Yoshimoto A., Ito M., Nunobiki S., Mantani Y., Yokoyama T., Hirano T., Ikenaka Y., Hoshi N.

  8th International Chemical Hazard Symposium, Oct. 2024, Japanese


• 「Transgenerational effects on maternal behavior due to exposure to a no-observed-adverse-effect-level (NOAEL) dose of neonicotinoid pesticide clothianidin

  Nunobiki S., Yoshimoto A., Ito M., Morishita R., Mantani Y., Yokoyama T., Hirano T., Ikenaka Y., Hoshi N.

  8th International Chemical Hazard Symposium, Oct. 2024, Japanese


• Paternal clothianidin exposure alters sperm miRNA profiles in mice and affects behavior in male offspring

  伊藤槙子, 布引沙利香, 吉本彩乃, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星信彦

  8th International Chemical Hazard Symposium, Oct. 2024, Japanese


• Developmental neurotoxicity of fipronil

  Yoshimoto A., Ito M., Nunobiki S., Hara Y., Ishida Y., Yonoichi S., Mantani Y., Yokoyama T., Hirano T., Ikenaka Y., Hoshi N.

  7th International Chemical Hazard Symposium, Dec. 2023, Japanese


• Transgenerational effects of maternal behavior induced by exposure to neonicotinoid pesticide

  Nunobiki S., Ito M., Yoshimoto A., Ishida Y., Hara Y., Yonoichi S., Mantani Y., Yokoyama T., Hirano T., Ikenaka Y., Hoshi N.

  7th International Chemical Hazard Symposium, Dec. 2023, Japanese


• ネオニコチノイド系農薬の父性曝露による精子miRNAの変動と次世代影響に関する研究

  伊藤槙子, 布引沙利香, 吉本彩乃, 原悠佳子, 石田祐也, 世ノ一さくら, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星信彦

  7th International Chemical Hazard Symposium, Dec. 2023, Japanese


• 発生中のマウスミュラー管の構造を維持する器官培養法の検討

  加藤栞, 横山俊史, 奥西宣祐, 成田翔, 桐月優輔, 藤川大誠, 万谷洋平, 星信彦

  第99回日本解剖学会近畿支部学術集会, Nov. 2023, Japanese

  Oral presentation


• 精巣化が脆弱なB6Js-XYPOSマウス性腺におけるSox9発現の消退

  桐月優輔,横山俊史,藤川大誠,加藤栞,犬塚千嘉,河合穂香,万谷洋平,星信彦

  第99回日本解剖学会近畿支部学術集会, Nov. 2023, Japanese

  Oral presentation


• クロチアニジンがアトピー性皮膚炎症状に及ぼす影響について

  石田祐也,世ノ一さくら,原悠佳子,伊藤槙子,布引沙利香,吉本彩乃,万谷洋平,横山俊史,池中良徳,星信彦

  第99回日本解剖学会近畿支部学術集会, Nov. 2023, Japanese

  Oral presentation


• ネオニコチノイド系農薬曝露による腸内細菌叢変動メカニズムに関する研究

  世ノ一さくら,石田祐也,原悠佳子,伊藤槙子,布引沙利香,吉本彩乃,平野哲史,万谷洋平,横山俊史,池中良徳,綾部時芳,中村公則,星信彦

  第99回日本解剖学会近畿支部学術集会, Nov. 2023, Japanese

  Oral presentation


• マウス未分化性腺の精巣化へのビタミンD受容体（Vdr）の関与

  藤川大誠,横山俊史,桐月優輔,犬塚千嘉,河合穂香,万谷洋平,星 信彦

  第99回日本解剖学会近畿支部学術集会, Nov. 2023, Japanese

  Oral presentation


• 発生中のマウスミュラー管尾部の器官培養法の検討

  加藤栞,横山俊史,奥西宣祐,成田翔,桐月優輔,藤川大誠,万谷洋平,星信彦

  第166回日本獣医学会学術集会, Sep. 2023, Japanese

  Oral presentation


• 胎子授乳期ネオニコチノイド系農薬曝露が海馬記憶と小脳運動学習機能に及ぼす継世代影響

  原悠佳子,世ノ一さくら,石田祐也,伊藤槙子,布引沙利香,吉本彩乃,万谷洋平,横山俊史,平野哲史,池中良徳,星信彦

  第166回日本獣医学会学術集会, Sep. 2023, Japanese

  Oral presentation


• ラットの回腸粘膜下組織における神経系の生後発生過程に関する組織学的研究

  森下理奈子,万谷洋平,中西怜稀,横山俊史,星信彦

  第166回日本獣医学会学術集会, Sep. 2023, Japanese

  Oral presentation


• 胎子・授乳期ネオニコチノイド系農薬曝露が海馬記憶と運動学習に及ぼす継世代影響

  原悠佳子,世ノ一さくら,石田祐也,正田明日香,村田碧,木村真子,万谷洋平,横山俊史,平野哲史,池中良徳,星信彦

  6th International Chemical Hazard Symposium, Jan. 2023, Japanese

  Oral presentation


• アトピー性皮膚炎におけるクロチアニジン曝露の影響評価

  石田祐也,原悠佳子,世ノ一さくら,正田明日香,村田碧,木村真子,万谷洋平,横山俊史,松尾栄子,星信彦

  6th International Chemical Hazard Symposium, Jan. 2023, Japanese

  Oral presentation


• ネオニコチノイド系農薬と環境ストレス負荷が腸内細菌叢に及ぼす影響

  世ノ一さくら,石田祐也,原悠佳子,木村真子,正田明日香,村田碧,万谷洋平,横山俊史,平野哲史,池中良徳,星信彦

  6th International Chemical Hazard Symposium, Jan. 2023, Japanese

  Oral presentation


• ジアミド系農薬クロラントラニリプロールがマウスの行動および神経活動に及ぼす影響

  木村真子, 正田明日香, 村田碧, 原悠佳子, 世ノ一さくら, 石田祐也, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星信彦

  6th International Chemical Hazard Symposium, Jan. 2023, Japanese

  Oral presentation


• Serial block-face 走査型電子顕微鏡を用いたラット大腸粘膜におけるマクロファージの超微形態学的部位差に関する研究

  村瀬翔大,万谷洋平,大野伸彦,中西怜稀,横山俊史,星信彦

  第98回日本解剖学会近畿支部学術集会, Nov. 2022, Japanese, Domestic conference


• ラット腸管における免疫担当細胞の局在への日常的な細菌刺激の影響に関する予備的解析

  島田晨香,万谷洋平,中西怜稀,横山俊史,星信彦

  第98回日本解剖学会近畿支部学術集会, Nov. 2022, Japanese, Domestic conference


• C57BL/6N マウスの未分化性腺における Sry 上流因子の発現に関する定量組織学的検討

  奥西宣祐,横山俊史,成田大翔,加藤栞,桐月優輔,藤川大誠,万谷洋平,星信彦

  第98回日本解剖学会近畿支部学術集会, Nov. 2022, Japanese, Domestic conference


• 性決定期の Sry 発現に対する C57BL/6 マウスの亜系統差の影響に関する定量組織学的解析

  成田大翔,横山俊史,奥西宣祐,加藤栞,桐月優輔,藤川大誠,万谷洋平,星信彦

  第98回日本解剖学会近畿支部学術集会, Nov. 2022, Japanese, Domestic conference


• アミド系農薬がマウスの行動および神経活動に及ぼす影響について

  木村真子,正田明日香,村田碧,原悠佳子,世ノ一さくら,石田祐也,平野哲史,万谷洋平,横山俊史,池中良徳,星信彦

  第98回日本解剖学会近畿支部学術集会, Nov. 2022, Japanese, Domestic conference


• 無毒性量クロチアニジンの経口曝露がアトピー性皮膚炎に与える影響の評価

  石田祐也,原悠佳子,世ノ一さくら,木村真子,正田明日香,村田碧,万谷洋平,横山俊史,松尾栄子,星信彦

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• 環境ストレス負荷下におけるネオニコチノイド系農薬曝露が腸内細菌叢に及ぼす影響

  世ノ一さくら,石田祐也,原悠佳子,木村真子,正田明日香,村田碧,万谷洋平,横山俊史,平野哲史,池中良徳,星信彦

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• 胎子授乳期ネオニコチノイド系農薬曝露が海馬記憶と運動学習に及ぼす次世代影響

  原悠佳子,世ノ一さくら,石田祐也,正田明日香,村田碧,木村真子,万谷洋平,横山俊史,平野哲史,池中良徳,星信彦

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• 性決定期のC57BL/6マウスにおけるSry発現の亜系統差に関する定量組織学的検討

  成田大翔,横山俊史,奥西宣祐,加藤栞,桐月優輔,藤川大誠,万谷洋平,星信彦

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• 性腺分化の臨界期のC57BL/6NマウスにおけるSry上流因子の発現に関する定量組織学的検討

  奥西宣祐,成田大翔,横山俊史,加藤栞,桐月優輔,藤川大誠,星信彦,万谷洋平

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• ラット回腸粘膜内神経ネットワークの生後変化に関する組織学的研究

  森下理奈子, 万谷洋平, 中西怜稀, 横山俊史, 星信彦

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• ラット大腸粘膜におけるマクロファージの組織学的部位差に関する研究

  村瀬翔大, 万谷洋平, 大野伸彦, 中西怜稀, 横山俊史, 星信彦

  第165回日本獣医学会学術集会, Sep. 2022, Japanese, Domestic conference


• Histological study on postnatal changes of the nerve network in the rat ileal mucosa.

  Morishita R, Mantani Y, Nakanishi S, Yokoyama T, Hoshi N

  International Federation of Associations of Anatomists 2022, Aug. 2022, English, Domestic conference


• 発生中のマウスにおける中腎領域へのAMHの移行経路の検討

  加藤 栞,横山俊史,奥西宣祐,成田大翔,桐月優輔,藤川大誠,万谷洋平,星 信彦

  日本解剖学会第97回近畿支部学術集会, Nov. 2021, Japanese, Domestic conference


• マウス胎子における精巣から中腎内へのAMHの移行経路の検討

  加藤 栞,横山俊史,奥西宣祐,成田大翔,桐月優輔,藤川大誠,万谷洋平,星 信彦

  第147回関西実験動物研究会, Dec. 2021, Japanese, Domestic conference


• 低用量クロチアニジンの神経回路形成期におけるステージ別曝露と発達神経毒性評価

  正田 明日香.村田 碧, 西 美咲, 平野 哲史, 万谷 洋平, 横山 俊史, 池中 良徳, 星 信彦

  第23回環境ホルモン学会研究発表会, Sep. 2021, Japanese, Domestic conference


• ネオニコチノイド系農薬クロチアニジンの胎子・授乳期曝露による次世代マウスの免疫系および腸内細菌叢の変化

  村田 碧, 西 美咲, 正田明日香, 池中良徳, 佐伯圭一, 松尾栄子, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

  第23回環境ホルモン学会研究発表会, Sep. 2021, Japanese, Domestic conference


• 極微量クロチアニジン曝露による神経学的影響の解明

  西美咲, 正田明日香, 村田碧, 杉尾翔太, 加藤大輔, 和氣弘明, 万谷洋平, 横山俊史, 星信彦

  第23回環境ホルモン学会研究発表会, Sep. 2021, Japanese, Domestic conference


• Serial block-face走査型電子顕微鏡を用いたラット大腸粘膜における神経ネットワークに関する研究

  中西 怜稀, 万谷 洋平, 大野 伸彦, 横山 俊史, 星 信彦

  第164回日本獣医学会学術集会, Sep. 2021, Japanese, Domestic conference


• 神経回路形成期におけるステージ別ネオニコチノイド系農薬曝露と発達神経毒性評価

  正田 明日香.村田 碧, 西 美咲, 平野 哲史, 万谷 洋平, 横山 俊史, 池中 良徳, 星 信彦

  第164回日本獣医学会学術集会, Sep. 2021, Japanese, Domestic conference


• ネオニコチノイド系農薬クロチアニジンの胎子・授乳期曝露が次世代マウスの免疫系および腸内細菌叢に及ぼす影響

  村田 碧, 西 美咲, 正田明日香, 佐伯圭一, 松尾栄子, 万谷洋平, 横山俊史, 平野哲史, 池中良徳, 星 信彦

  第164回日本獣医学会学術集会, Sep. 2021, Japanese, Domestic conference


• クロチアニジン曝露による高次脳機能の維持破綻メカニズムの解明

  西美咲, 正田明日香, 村田碧, 杉尾翔太, 加藤大輔, 和氣弘明, 万谷洋平, 横山俊史, 星信彦

  第164回日本獣医学会学術集会, Sep. 2021, Japanese, Domestic conference


• 発生中のマウス精巣から中腎領域へのAMHの移行経路の検討

  加藤 栞,横山俊史,奥西宣祐,成田大翔,桐月優輔,藤川大誠,万谷洋平,星 信彦

  第164回日本獣医学会学術集会, Sep. 2021, Japanese, Domestic conference


• ラット腸管における粘膜内グリア細胞に関する組織学的研究

  万谷洋平, 中西怜稀, 大野伸彦, 春田知洋, 横山俊史, 星 信彦

  第164回日本獣医学会学術集会, Sep. 2021, Japanese, Domestic conference


• ネオニコチノイド系農薬クロチアニジンのマウス母乳中移行量に関する定量的評価

  正田 明日香.村田 碧, 西 美咲, 平野 哲史, 万谷 洋平, 横山 俊史, 池中 良徳, 星 信彦

  第61回日本先天異常学会学術集会, Aug. 2021, Japanese, Domestic conference


• ネオニコチノイド系農薬クロチアニジンが次世代マウスの免疫系および腸内細菌叢に及ぼす影響

  村田 碧, 西 美咲, 正田明日香, 池中良徳, 佐伯圭一, 松尾栄子, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

  第61回日本先天異常学会学術集会, Aug. 2021, Japanese, Domestic conference


• 2光子顕微鏡を用いたクロチアニジン曝露による神経学的影響の解明

  西美咲, 正田明日香, 村田碧, 杉尾翔太, 加藤大輔, 和氣弘明, 万谷洋平, 横山俊史, 星信彦

  第61回日本先天異常学会学術集会, Aug. 2021, Japanese, Domestic conference


• 性分化撹乱マウス系統（B6J-XYPOS）における性腺原基の卵巣化に関する免疫組織化学的研究

  中村大河,横山俊史,三浦由佳,加藤栞,奥西宣祐,成田大翔,万谷洋平,星信彦

  第96回日本解剖学会近畿支部学術集会, Nov. 2020, Japanese, Domestic conference


• ラット消化管粘膜上皮表面における常在細菌の定着程度の概日リズムに関する定量組織学的解析

  坂田奈那美,万谷洋平,中西怜稀,横山俊史,星 信彦

  第96回日本解剖学会近畿支部学術集会, Nov. 2020, Japanese, Domestic conference


• 極微量ネオニコチノイド系農薬がマウスの雌性生殖器に及ぼす継世代影響

  北内 佐也可, 前田 瑞稀, 西 美咲, 正田 明日香, 村田 碧, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  日本獣医学会学術集会講演要旨集, Oct. 2020, Japanese, (公社)日本獣医学会


• ラット大腸粘膜における線維芽細胞様細胞の部位差に関する組織学的研究

  万谷洋平, 田村彩貴, 大野伸彦, 横山俊史, 星 信彦.

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference


• 無毒性量ネオニコチノイド系農薬がマウスの継世代雌性生殖器に及ぼす影響

  北内 佐也可,前田 瑞稀,西 美咲,正田 明日香,村田 碧,平野 哲史,万谷 洋平,横山 俊史,星 信彦

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference


• 性分化撹乱マウス系統（B6J-XYPOS）における精巣化破綻機構に関する免疫組織化学的研究

  中村大河,横山俊史,三浦由佳,加藤栞,奥西宣祐,成田大翔,万谷洋平,星信彦

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference


• ラット消化管各部位の粘膜上皮表面における常在細菌の定着程度の日内変動に関する組織学的解析

  坂田奈那美,万谷洋平,中西怜稀,横山俊史,星 信彦

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference


• 腸管各部位の集合リンパ小節における細網細胞の部位差に関する免疫組織化学的研究

  土居萌花,万谷洋平,中西怜稀,坂田奈那美,横山俊史,星 信彦

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference


• 無毒性量ネオニコチノイド系農薬が次世代のマウス雌性生殖機能に及ぼす影響

  北内 佐也可,前田 瑞稀,平野 哲史,万谷 洋平,横山 俊史,星 信彦

  第60回日本先天異常学会学術集会, Jul. 2020, Japanese, Domestic conference


• 無毒性量の浸透性農薬のマウス胎子・授乳期曝露による神経行動学的影響

  前田瑞稀,北内佐也加,平野哲史,万谷洋平,横山俊史,星 信彦

  第60回日本先天異常学会学術集会, Jul. 2020, Japanese, Domestic conference


• 低用量クロチアニジンによる情動変容への加齢の影響評価

  宮田結佳, 久保静花, 大野周嗣, 大成果乃子, 前田瑞稀, 中西怜稀, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星信彦

  Endocrine Disrupter News Letter (Web), 2020


• ネオニコチノイド系農薬における野生哺乳動物の曝露状況と都道府県別の出荷量との関連．

  杉田晄佑, 高田 匡, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星 信彦.

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• ネオニコチノイド系農薬クロチアニジンが次世代雌性生殖器に及ぼす影響．

  北内佐也可, 前田瑞稀, 久保静花, 大成果乃子, 宮田結佳, 平野哲史, 万谷洋平, 横山俊史, 星 信彦.

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• クロチアニジンおよびその代謝産物の母子間移行における組織分布・蓄積性の定量的解明．

  大野周嗣, 大成果乃子, 久保静花, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星 信彦.

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• 低用量クロチアニジンの胎子・授乳期曝露による神経行動学的影響．

  前田瑞稀, 北内佐也可, 宮田結佳, 久保静花, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• 低用量クロチアニジンによる情動変容への加齢の影響評価．

  宮田結佳, 久保静花, 大野周嗣, 大成果乃子, 前田瑞稀, 中西怜稀, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星 信彦

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• クロチアニジンの亜急性摂取はラット腸内細菌叢を変化させる．

  大成果乃子, 大野周嗣, 久保静花, 中西怜稀, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• 低用量クロチアニジンによるマウスの物体認識・空間学習記憶障害とその性差．

  久保静花, 宮田結佳, 大成果乃子, 大野周嗣, 平野哲史, 万谷洋平, 横山俊史, 星 信彦.

  環境ホルモン学会第22回研究発表会, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• ネオニコチノイド系農薬クロチアニジンが次世代雌性生殖器に及ぼす影響

  北内 佐也可, 前田 瑞稀, 久保 静花, 大成 果乃子, 宮田 結佳, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2019, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 低用量クロチアニジンの胎子・授乳期曝露による神経行動学的影響

  前田 瑞稀, 北内 佐也可, 宮田 結佳, 久保 静花, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2019, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 低用量クロチアニジンによるマウスの物体認識・空間学習記憶障害とその性差

  久保 静花, 宮田 結佳, 大成 果乃子, 大野 周嗣, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2019, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• クロチアニジンおよびその代謝産物の母子間移行における組織分布・蓄積性の定量的解明

  大野 周嗣, 大成 果乃子, 久保 静花, 平野 哲史, 万谷 洋平, 横山 俊史, 石塚 真由美, 池中 良徳, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2019, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• クロチアニジンの亜急性摂取はラット腸内細菌叢を変化させる

  大成 果乃子, 大野 周嗣, 久保 静花, 中西 怜稀, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2019, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 低用量クロチアニジンによる情動変容への加齢の影響評価

  宮田 結佳, 久保 静花, 大野 周嗣, 大成 果乃子, 前田 瑞稀, 中西 怜稀, 平野 哲史, 万谷 洋平, 横山 俊史, 石塚 真由美, 池中 良徳, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2019, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• Left-right asymmetry of Sry expression in the developing gonad of C57BL/6N mouse.

  Kohei KAWANISHI, Chinatsu HASEGAWA, Taiga NAKAMURA, Shiori KATOH, Yohei MANTANI, Toshifumi YOKOYAMA, Nobuhiko HOSHI

  The 42nd Annual Meeting of the Molecular Biology Society of Japan, Dec. 2019, English, Fukuoka, International conference

  Poster presentation


• Testis differentiation process of Y^POS mice with fragile Sry is affected by genetic differences in C57BL/6 substrains.

  Toshifumi YOKOYAMA, Yuuka MIURA, Anzu YAMAMOTO, Chinatsu HASEGAWA, Kohei KAWANISHI, Nanako TAKADA, Takuya OMOTEHARA, Tetsushi HIRANO, Yohei MANTANI, Takanori MIKI, Nobuhiko HOSHI

  The 42nd Annual Meeting of the Molecular Biology Society of Japan, Dec. 2019, English, Fukuoka, International conference

  Poster presentation


• マウス健常雄個体の性腺におけるSry 発現の左右差に関する定量組織学的研究

  川西 航平, 長谷川 千夏, 中村 大河, 加藤 栞, 万谷 洋平, 横山 俊史, 星 信彦

  第95回日本解剖学会近畿支部学術集会, Nov. 2019, Japanese, 大阪市, Domestic conference

  Oral presentation


• Serial block-face 走査型電子顕微鏡を用いたラット回腸粘膜における神経接続の三次元解析

  中西 怜稀, 万谷 洋平, 春田 知洋, 横山 俊史, 星 信彦

  第95回日本解剖学会近畿支部学術集会, Nov. 2019, Japanese, 大阪市, Domestic conference

  Oral presentation


• ラット小腸粘膜における好酸球の多様性に関する組織学的研究

  荒井真也, 万谷洋平, 中西怜稀, 春田知洋, 西田美穂, 湯浅秀人, 横山俊史, 星信彦, 北川浩

  第95回日本解剖学会近畿支部学術集会, Nov. 2019, Japanese, 大阪市, Domestic conference

  Oral presentation


• GABA撹乱物質フィプロニルがマウスの情動行動に及ぼす影響

  前田 瑞稀, 大成 果乃子, 大野 周嗣, 久保 静花, 杉田 晄佑, 宮田 結佳, 北内 佐也加, 西 美咲, 高田 匡, 平野 哲史, 万谷 洋平, 横山 俊史, 池中 良徳, 星 信彦

  第162回日本獣医学会学術集会, Sep. 2019, Japanese, つくば市, Domestic conference

  Oral presentation


• 性決定期のマウス性腺におけるSry発現の時空間的解析-健常雄個体におけるSry発現の左右差

  川西 航平, 長谷川 千夏, 中村 大河, 加藤 栞, 万谷 洋平, 横山 俊史, 星 信彦

  第162回日本獣医学会学術集会, Sep. 2019, Japanese, つくば市, Domestic conference

  Oral presentation


• 性決定前のマウス未分化XY性腺の精巣化を誘導する器官培養法の確立とその組織学的評価

  長谷川 千夏, 梅村 ゆりあ, 川西 航平, 中村 大河, 加藤 栞, 万谷 洋平, 横山 俊史, 星 信彦

  第162回日本獣医学会学術集会, Sep. 2019, Japanese, つくば市, Domestic conference

  Oral presentation


• Serial block-face走査型電子顕微鏡(SBF-SEM)を用いたラット回腸の粘膜固有層における 神経投射対象の解明

  中西怜稀, 万谷洋平, 春田知洋, 横山俊史, 星信彦

  第162回日本獣医学会学術集会, Sep. 2019, Japanese, つくば市, Domestic conference

  Oral presentation


• Serial block-face走査型電子顕微鏡を用いたラット回腸における単核食細胞系列の細胞と上皮 内リンハ?球の相互作用に関する研究

  万谷洋平, 春田知洋, 中西怜稀, 片田裕亮, 横山俊史, 星信彦, 北川浩

  第162回日本獣医学会学術集会, Sep. 2019, Japanese, つくば市, Domestic conference

  Oral presentation


• GABA攪乱物質フィプロニルがマウスの情動行動に及ぼす影響

  前田 瑞稀, 大成 果乃子, 大野 周嗣, 久保 静花, 杉田 晄佑, 宮田 結佳, 北内 佐也加, 西 美咲, 高田 匡, 平野 哲史, 万谷 洋平, 横山 俊史, 池中 良徳, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2019, Japanese, (公社)日本獣医学会


• Three-Dimensional Observation of Mononuclear Phagocyte Lineages in the Rat Ileum using Serial Block-Face Scanning Electron Microscopy

  Youhei MANTANI, Tomohiro HARUTA, Satoki NAKANISHI, Yusuke KATADA, Toshifumi YOKOYAMA, Nobuhiko HOSHI, Hiroshi KITAGAWA

  19th International Congress of Mucosal Immunology, Jul. 2019, English, Brisbane, International conference

  Poster presentation


• ヒト神経芽細胞腫におけるネオニコチノイド系農薬のシグナル毒性的影響評価と作用機序の解析

  平野 哲史, 皆川 沙月, 古澤 之裕, 柚木 達也, 池中 良徳, 横山 俊史, 星 信彦, 田渕 圭章

  The Journal of Toxicological Sciences, Jun. 2019, Japanese, (一社)日本毒性学会


• In vivo and in vitro effects of a neonicotinoid pesticide, clothianidin, on mammalian nervous function

  Tetsushi Hirano, Satsuki Minagawa, Yukihiro Furusawa, Tatsuya Yunoki, Toshifumi Yokoyama, Nobuhiko Hoshi, Yoshiaki Tabuchi

  IUTOX 15th International Congress of Toxicology, Jun. 2019, English, Honolulu, International conference

  Poster presentation


• ネオニコチノイド系農薬がヒト神経芽細胞腫に及ぼすシグナル毒性的影響に関するメカニズム解析

  平野 哲史, 皆川 沙月, 古澤 之裕, 柚木 達也, 池中 良徳, 横山 俊史, 星 信彦, 田渕 圭章

  第46回日本毒性学会, Jun. 2019, Japanese, 徳島市, Domestic conference

  Oral presentation


• C57BL/6マウスの亜系統差による生殖腺表現型の変化

  三浦由佳, YOKOYAMA TOSHIFUMI, 山本杏, 川西航平, 長谷川千夏, 高田菜々子, MANTANI YOUHEI, HOSHI NOBUHIKO

  第32回モロシヌス研究会, Jun. 2019, Japanese, 千葉市, Domestic conference

  Oral presentation


• Serial Block face走査型電子顕微鏡を用いたラット回腸粘膜における単核食細胞系列の細胞の三次元的観察

  MANTANI YOUHEI, 春田知洋, 中西怜稀, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第124回日本解剖学会総会全国学術集会, Mar. 2019, Japanese, 新潟市, Domestic conference

  Poster presentation


• Adverse effects on cognitive-emotional behavior and immune system function in experimental animals administered a NOAEL-dose of neonicotinoids.

  HOSHI NOBUHIKO, YOSHINORI IKENAK, SHUJI OHNO, KANOKO ONARU, SHIZUKA KUBO, MIZUKI MAEDA, MIYATA YUKA, SUGITA KOUSUKE, MAYUMI ISHIZUKA, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI

  Chemical Hazard Symposium in Hokkaido, Mar. 2019, English, Sapporo, International conference

  [Invited]

  Nominated symposium


• 無毒性量のネオニコチドの系濃薬曝露による行動影響の性差

  久保静花, 宮田結佳, 大成果乃子, 大野周嗣, 高田匡, 平野哲史, 横山俊史, 星信彦

  Endocrine Disrupter News Letter, 2019


• Survey on exposure of neonicotinoid pesticide in wild mammals

  杉田晄佑, 高田匡, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星信彦

  環境ホルモン学会研究発表会プログラム・要旨集, 2019


• Mechanism analysis of signal toxic effects of neonicotinoid pesticides on human neuroblastoma cells

  HIRANO Tetsushi, MINAGAWA Satsuki, FURUSAWA Yukihiro, YUNOKI Tatsuya, IKENAKA Yoshinori, YOKOYAMA Toshifumi, HOSHI Nobuhiko, TABUCHI Yoshiaki

  Annual Meeting of the Japanese Society of Toxicology, 2019, Japanese, The Japanese Society of Toxicology,

  [in Japanese]


• ネオニコチノイド系農薬クロチアニジンはヒト神経芽細胞腫においてカルシウムシグナルのかく乱を引き起こす

  平野 哲史, 皆川 沙月, 古澤 之裕, 柚木 達也, 横山 俊史, 星 信彦, 田渕 圭章

  環境ホルモン学会研究発表会要旨集, Dec. 2018, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 無毒性量のネオニコチノイド系農薬曝露による行動影響の性差

  久保静花, 宮田結佳, 大成果乃子, 大野周嗣, 高田匡, 平野哲史, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

  Poster presentation


• 母子間移行における浸透性農薬およびその代謝産物の定量的な解明

  大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, 石塚真由美, 池中良徳, HOSHI NOBUHIKO

  環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

  Poster presentation


• 兵庫県における野生哺乳動物のネオニコチノイド系農薬への曝露実態調査

  杉田晄佑, 高田匡, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, 石塚真由美, 池中良徳, HOSHI NOBUHIKO

  環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

  Poster presentation


• 性決定遺伝子発現前のマウス未分化XY性腺の精巣化を誘導する器官培養法

  長谷川千夏, 梅村ゆりあ, 三浦由佳, 川西航平, 高田菜々子, 大野周嗣, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  関西実験動物研究会第140回研究会, Dec. 2018, Japanese, 京都市, Domestic conference

  Oral presentation


• ラットにおけるネオニコチノイド系農薬の亜急性曝露による免疫毒性の検証

  大成果乃子, 大野周嗣, 久保静花, 中西怜稀, 荒井真也, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

  Poster presentation


• ネオニコチノイド系農薬が老年動物に及ぼす影響

  宮田結佳, 久保静花, 大野周嗣, 前田瑞稀, 荒井真也, 中西怜稀, 高田匡, 平野哲史, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

  Poster presentation


• 性決定前のマウス未分化 XY 性腺の精巣化を誘導する器官培養法の確立とその組織学的評価

  長谷川千夏, 梅村ゆりあ, 三浦由佳, 川西航平, 高田菜々子, 大野周嗣, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第94回日本解剖学会近畿支部学術集会, Nov. 2018, Japanese, 神戸市, Domestic conference

  Oral presentation


• ラット回腸粘膜における好酸球系列の細胞の組織学的研究

  荒井真也, MANTANI YOUHEI, 西田美穂, 湯浅秀人, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第94回日本解剖学会近畿支部学術集会, Nov. 2018, Japanese, 神戸市, Domestic conference

  Oral presentation


• ラット回腸の上皮下線維芽細胞様細胞を制御し得る神経伝達物質の同定

  中西怜稀, MANTANI YOUHEI, 田村彩貴, 荒井真也, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第94回日本解剖学会近畿支部学術集会, Nov. 2018, Japanese, 神戸市, Domestic conference

  Oral presentation


• 生殖腺の表現型に及ぼすマウス亜系統差の影響に関する遺伝学的研究

  三浦由佳, 山本杏, 川西航平, 長谷川千夏, 高田菜々子, 高田匡, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• 浸透性農薬の行動影響の性差

  久保静花, 宮田結佳, 大成果乃子, 大野周嗣, 杉田晄佑, 前田瑞稀, 高田匡, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• 浸透性農薬とその代謝産物の胎子移行量の解明

  大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 杉田晄佑, 宮田結佳, 高田匡, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, 石塚真由美, 池中良徳, HOSHI NOBUHIKO

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• ラット小腸粘膜におけるCD11b陽性細胞を中心とした各種免疫担当細胞の組織内配置に関する免疫組織化学的研究

  荒井真也, MANTANI YOUHEI, 西田美穂, 湯浅秀人, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• ラット呼吸器系粘膜におけるSP-AおよびSP-D分泌細胞の分布

  斉旺梅, 増田なつみ, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• ラット回腸腸絨毛における上皮下および非上皮下線維芽細胞様細胞の三次元的解析

  MANTANI YOUHEI, 春田知洋, 西田美穂, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• ラット回腸の上皮直下における線維芽細胞様細胞への神経投射に関する免疫組織化学的研究

  中西怜稀, MANTANI YOUHEI, 田村彩貴, 荒井真也, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬における哺乳動物への免疫毒性の検証

  大成果乃子, 大野周嗣, 久保静花, 中西怜稀, 荒井真也, 杉田晄佑, 宮田結佳, 高田匡, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬が老齢動物に及ぼす影響

  宮田結佳, 大成果乃子, 大野周嗣, 久保静花, 杉田晄佑, 高田匡, 前田瑞稀, 中西怜稀, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

  Oral presentation


• 浸透性農薬とその代謝産物の胎子移行量の解明

  大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 杉田晄佑, 宮田結佳, 高田匡, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星信彦

  日本獣医学会学術集会講演要旨集, Aug. 2018, Japanese


• 浸透性農薬の行動影響の性差

  久保 静花, 宮田 結佳, 大成 果乃子, 大野 周嗣, 杉田 晄佑, 前田 瑞稀, 高田 匡, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2018, Japanese, (公社)日本獣医学会


• ネオニコチノイド系農薬における哺乳動物への免疫毒性の検証

  大成 果乃子, 大野 周嗣, 久保 静花, 中西 怜稀, 荒井 真也, 杉田 晄佑, 宮田 結佳, 高田 匡, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2018, Japanese, (公社)日本獣医学会


• ネオニコチノイド系農薬が老齢動物に及ぼす影響

  宮田 結佳, 大成 果乃子, 大野 周嗣, 久保 静花, 杉田 晄佑, 高田 匡, 前田 瑞稀, 中西 怜稀, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2018, Japanese, (公社)日本獣医学会


• ニワトリ胚におけるセルトリ細胞の起源(The origin of Sertoli cells in the chicken embryo)

  表原 拓也, 永堀 健太, 河田 晋一, 李 忠連, 宮宗 秀伸, 伊藤 正裕, 南 貴一, 万谷 洋平, 梅村 ゆりあ, 西田 美穂, 平野 哲史, 北川 浩, 横山 俊史, 星 信彦, 吉岡 秀文

  東京医科大学雑誌, Jan. 2018, English, 東京医科大学医学会


• ニワトリ胚におけるセルトリ細胞の起源(The origin of Sertoli cells in the chicken embryo)

  表原 拓也, 永堀 健太, 河田 晋一, 李 忠連, 宮宗 秀伸, 伊藤 正裕, 南 貴一, 万谷 洋平, 梅村 ゆりあ, 西田 美穂, 平野 哲史, 北川 浩, 横山 俊史, 星 信彦, 吉岡 秀文

  東京医科大学雑誌, Jan. 2018, English, 東京医科大学医学会


• 発達期におけるネオニコチノイド系農薬曝露により引き起こされたドーパミン神経系の攪乱を伴う多動

  米田直起, 高田 匡, 大成果乃子, 大野周嗣, 久保静花, 杉田晄佑, 宮田結佳, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  環境ホルモン学会第20回研究発表会, Dec. 2017, Japanese, 神戸市, Domestic conference

  Poster presentation


• 環境ストレスおよびネオニコチノイド系農薬ジノテフランの複合影響評価

  高田 匡, 米田直起, 大成果乃子, 大野周嗣, 久保静花, 杉田晄佑, 宮田結佳, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  環境ホルモン学会第20回研究発表会, Dec. 2017, Japanese, 神戸市, Domestic conference

  Poster presentation


• マウスの亜系統差が生殖腺に及ぼす影響の遺伝学的研究

  三浦由佳, 山本 杏, 川西航平, 長谷川千夏, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第136回関西実験動物研究会, Dec. 2017, Japanese, 京都市, Domestic conference

  Oral presentation


• ラット大腸の上皮直下における間質細胞の部位差に関する免疫組織化学的研究

  田村彩貴, MANTANI YOUHEI, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第93回日本解剖学会近畿支部学術集会, Nov. 2017, Japanese, 大津市, Domestic conference

  Oral presentation


• ラット回腸における間質細胞を介した細胞間ネットワーク解析へのSerial Block Face走査型電子顕微鏡の有用性

  MANTANI YOUHEI, 春田知洋, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第93回日本解剖学会近畿支部学術集会, Nov. 2017, Japanese, 大津市, Domestic conference

  Oral presentation


• マウスのミュラー管退行におけるAMH作用様式

  山本杏, 表原拓也, 三浦由佳, 川西航平, 長谷川千夏, MANTANI YOUHEI, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第93回日本解剖学会近畿支部学術集会, Nov. 2017, Japanese, 大津市, Domestic conference

  Oral presentation


• 生理的条件下のラット呼吸器系粘膜における補体成分C3と補体受容体C3aRおよびCD11bの局在に関する免疫組織化学的研究

  吉冨千晶, 増田なつみ, 湯浅秀人, 西田美穂, MANTANI YOUHEI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第160回日本獣医学会学術集会, Sep. 2017, Japanese, 鹿児島市, Domestic conference

  Oral presentation


• ラットパイエル板におけるlysozyme陽性細胞を介する生体防御機構に関する免疫組織化学的研究

  宮本和樹, MANTANI YOUHEI, 湯浅秀人, 西田美穂, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第160回日本獣医学会学術集会, Sep. 2017, Japanese, 鹿児島市, Domestic conference

  Oral presentation


• Serial block face-走査型電子顕微鏡を用いたラット回腸腸陰窩周囲の間質細胞に関する3次元超微形態学的解析

  MANTANI YOUHEI, 春田知洋, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第160回日本獣医学会学術集会, Sep. 2017, Japanese, 鹿児島市, Domestic conference

  Oral presentation


• ニワトリの発生過程に特徴的な生殖腺の左右差

  表原拓也, 南貴一, MANTANI YOUHEI, 朝克吉楽, 梅村ゆりあ, 西田美穂, 平野哲史, 青山裕彦, 吉岡秀文, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第122回日本解剖学会総会・全国学術集会, Mar. 2017, Japanese, 長崎市, Domestic conference

  [Invited]

  Invited oral presentation


• ネオニコチノイド系農薬クロチアニジンは胎子型と成獣型、2種類のライディッヒ細胞にそれぞれ異なる影響を及ぼす

  柳井 翔吾, 平野 哲史, 表原 拓也, 高田 匡, 米田 直起, 岩本 遥, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2016, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 胎子期および発達期におけるネオニコチノイド系農薬曝露がドーパミン神経系および行動に及ぼす影響

  米田直起, 平野哲史, 柳井翔吾, 高田匡, 岩本遥, 表原拓也, 片田裕亮, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

  Poster presentation


• マウス胎子性腺における性分化関連遺伝子転写調節領域のDNAメチル化状態

  久保田直人, 表原拓也, 山本杏, 三浦由佳, 平野哲史, 南貴一, 柳井翔吾, 高田匡, 米田直起, 岩本遥, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第39回日本分子生物学会年会, Dec. 2016, Japanese, 横浜市, Domestic conference

  Poster presentation


• ネオニコチノイド系農薬曝露によりみられた神経行動学的異常および標的脳領域の探索

  平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 南貴一, 久保田直人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬ジノテフラン曝露はうつ様行動を引き起こすか？

  高田匡, 平野哲史, 柳井翔吾, 米田直起, 岩本遥, 表原拓也, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬クロチアニジンは胎子型と成獣型，2種類のライディッヒ細胞にそれぞれ異なる影響を及ぼす

  柳井翔吾, 平野哲史, 表原拓也, 高田匡, 米田直起, 岩本遥, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

  Oral presentation


• ラット呼吸器系粘膜を覆う粘液層における β-defensin 1 および β-defensin 2 の存在および分泌源の同定

  増田なつみ, MANTANI YOUHEI, 西田美穂, 湯浅秀人, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

  Oral presentation


• ラット回腸粘膜における各種間質細胞の局在

  MANTANI YOUHEI, 宮本和樹, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

  Oral presentation


• ラットパイエル板における濾胞被蓋上皮のアポトーシスとRANK/RANKLの発現との関係

  湯浅秀人, MANTANI YOUHEI, 増田なつみ, 西田美穂, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

  Oral presentation


• マウス真性半陰陽個体のミュラー管退行とAMHの作用様式

  山本杏, 表原拓也, 久保田直人, 南貴一, 平野哲史, MANTANI YOUHEI, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

  Oral presentation


• ニワトリ雌右性腺退縮メカニズムの解明

  南貴一, 表原拓也, 田渕圭章, 平野哲史, YOKOYAMA TOSHIFUMI, MANTANI YOUHEI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

  Oral presentation


• 生理学的条件下のラットパイエル板におけるRANK/RANKLの発現および常在細菌の増殖による影響

  湯浅秀人, 増田なつみ, 西田美穂, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, MANTANI YOUHEI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• ラット呼吸器におけるβ-defensin 1およびβ-defensin 2を介した分泌型の生体防御機構に関する免疫組織化学的研究

  増田なつみ, MANTANI YOUHEI, 西田美穂, 湯浅秀人, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• ラット回腸におけるtelocyteの免疫組織化学的同定

  MANTANI YOUHEI, 宮本和樹, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• マウス真性半陰陽個体におけるAMHの作用様式に関する組織学的解析

  山本杏, 表原拓也, 久保田直人, 南貴一, 広川千英, 平野哲史, 磯江美智子, 柳井翔吾, 高田匡, 米田直起, 三浦由佳, 岩本遥, MANTANI YOUHEI, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬曝露とうつ発症との関連性の検証

  高田匡, 平野哲史, 柳井翔吾, 米田直起, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬ジノテフランによるドーパミン神経系の攪乱および多動性の誘発

  米田直起, 平野哲史, 柳井翔吾, 高田匡, 表原拓也, 久保田直人, 南貴一, 山本杏, 岩本遥, 三浦由佳, 磯江美智子, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬クロチアニジン急性投与が及ぼす行動学的影響とその作用点

  平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 久保田直人, 南貴一, 山本杏, 岩本遥, 三浦由佳, 磯江美智子, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬とうつ発症との関連性の検証

  高田 匡, 平野 哲史, 柳井 翔吾, 米田 直起, 表原 拓也, 久保田 直人, 南 貴一, 山本 杏, 岩本 遥, 三浦 由佳, 磯江 美智子, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2016, Japanese, (公社)日本獣医学会


• ネオニコチノイド系農薬ジノテフランによるドーパミン神経系の攪乱および多動性の誘発

  米田 直起, 平野 哲史, 柳井 翔吾, 高田 匡, 表原 拓也, 久保田 直人, 南 貴一, 山本 杏, 岩本 遥, 三浦 由佳, 磯江 美智子, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2016, Japanese, (公社)日本獣医学会


• ネオニコチノイド系農薬クロチアニジン急性投与が及ぼす行動学的影響とその作用点

  平野 哲史, 柳井 翔吾, 高田 匡, 米田 直起, 表原 拓也, 久保田 直人, 南 貴一, 山本 杏, 岩本 遥, 三浦 由佳, 磯江 美智子, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2016, Japanese, (公社)日本獣医学会


• マウス真性半陰陽個体におけるAMHの作用様式に関する組織学的解析

  山本 杏, 表原 拓也, 久保田 直人, 南 貴一, 広川 千英, 平野 哲史, 磯江 美智子, 柳井 翔吾, 高田 匡, 米田 直起, 三浦 由佳, 岩本 遥, 万谷 洋平, 北川 浩, 横山 俊史, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2016, Japanese, (公社)日本獣医学会


• マウス未分化性腺におけるSry発現制御に関わる新規遺伝子の時空間特異的探索

  梅村 ゆりあ, 横山 俊史, 田渕 圭章, 南 貴一, 表原 拓也, 久保田 直人, 山本 杏, 広川 千英, 平野 哲史, 柳井 翔吾, 米田 直起, 高田 匡, 万谷 洋平, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2016, Japanese, (公社)日本獣医学会


• 発達期におけるネオニコチノイド系農薬曝露により多動が引き起こされる

  米田直起, 平野哲史, 柳井翔吾, 高田匡, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 広川千英, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第56回 先天異常学会学術集会, Jul. 2016, Japanese, 姫路市, Domestic conference

  Poster presentation


• 発達期におけるネオニコチノイド系農薬ジノテフラン曝露とうつ病発症との関係

  高田匡, 平野哲史, 柳井翔吾, 米田直起, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 広川千英, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第56回 先天異常学会学術集会, Jul. 2016, Japanese, 姫路市, Domestic conference

  Poster presentation


• 胎子期から成獣期にかけてのネオニコチノイド系農薬クロチアニジン曝露が及ぼす行動学的影響

  平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 広川千英, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第56回 先天異常学会学術集会, Jul. 2016, Japanese, 姫路市, Domestic conference

  Poster presentation


• ネオニコチノイド系農薬クロチアニジン単回投与によりみられた異常啼鳴および神経行動学的影響

  平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 久保田直人, 南貴一, 広川千英, 山本杏, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第43回日本毒性学会学術年会, Jun. 2016, Japanese, 名古屋市, Domestic conference

  Poster presentation


• ニワトリ胚の性腺発生における左右差

  表原拓也, 南貴一, 万谷洋平, 梅村ゆりあ, 西田 美穂, 平野哲史, 吉岡 秀文, 北川浩, 横山俊史, 星信彦

  第121回日本解剖学会総会・全国学術集会, Mar. 2016, Japanese, 郡山市, Domestic conference

  Oral presentation


• 環境ストレス下においてネオニコチノイド系農薬クロチアニジンが与える神経行動学的影響

  平野哲史, 柳井翔吾, 表原拓也, 高田匡, 米田直起, 梅村ゆりあ, 久保田直人, 南貴一, 広川千英, 山本杏, 万谷洋平, 横山俊史, 北川浩, 星信彦

  環境ホルモン学会第18回研究発表会, Dec. 2015, Japanese, 下野市, Domestic conference

  Poster presentation


• ネオニコチノイド系農薬クロチアニジンの妊娠期及び授乳期曝露がマウス産子の雄性生殖器に与える影響

  柳井翔吾, 平野哲史, 表原拓也, 高田匡, 米田直起, 梅村ゆりあ, 久保田直人, 南貴一, 広川千英, 山本杏, 万谷洋平, 横山俊史, 北川浩, 星信彦

  環境ホルモン学会第18回研究発表会, Dec. 2015, Japanese, 下野市, Domestic conference

  Poster presentation


• ニワトリ胚の左精巣に特異的なセルトリ細胞の起源

  表原拓也, 万谷洋平, 南貴一, 梅村ゆりあ, 平野哲史, 横山俊史, 北川浩, 星信彦

  第38回日本分子生物学会年会・第88回日本生化学会大会合同大会, Dec. 2015, Japanese, 神戸市, Domestic conference

  Oral presentation


• 生理学的条件下におけるラットパイエル板濾胞被蓋上皮におけるToll-like receptor-4の発現とM細胞への分化との関係について

  湯浅秀人, 万谷洋平, 増田なつみ, 西田美穂, 河野潤一, 横山俊史, 星信彦, 北川浩

  第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

  Oral presentation


• 性逆転系統（B6-XYPOS）マウスにおける性腺分化と遺伝的背景

  梅村ゆりあ, 表原拓也, 平野哲史, 久保田直人, 柳井翔吾, 増田なつみ, 湯浅秀人, 万谷洋平, 北川浩, 横山俊史, 星信彦

  第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

  Oral presentation


• ラット呼吸器系粘膜を覆う液相に含まれるlysozymeおよびsPLA2の分泌源の特定

  増田なつみ, 万谷洋平, 西田美穂, 湯浅秀人, 河野潤一, 横山俊史, 星信彦, 北川浩

  第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

  Oral presentation


• ラット回腸における間質細胞telocyteに関する超微形態学的研究

  万谷洋平, 西田美穂, 湯浅秀人, 増田なつみ, 横山俊史, 星信彦, 北川浩

  第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

  Oral presentation


• ニワトリ胚の左性腺に特異的な細胞運命の追跡

  表原拓也, 南貴一, 万谷洋平, 梅村ゆりあ, 西田美穂, 平野哲史, 吉岡秀文, 横山俊史, 北川浩, 星信彦

  第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

  Oral presentation


• ニワトリの性腺形態における左右差形成メカニズムの解明

  南 貴一, 表原拓也, 田渕圭章, 梅村ゆりあ, 平野哲史, 横山俊史, 北川 浩, 星 信彦

  第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

  Oral presentation


• 環境ストレス下において浸透性農薬クロチアニジンが行動及び生殖機能に与える影響

  平野哲史, 柳井翔吾, 表原拓也, 梅村ゆりあ, 久保田直人, 南貴一, 高田匡, 米田直起, 広川千英, 山本杏, 万谷洋平, 横山俊史, 北川浩, 星信彦

  第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

  Oral presentation


• ラット小腸のPaneth細胞に連絡する粘膜固有層細胞の超微形態

  万谷洋平, 西田美穂, 湯浅秀人, 増田なつみ, 横山俊史, 星信彦, 北川浩

  第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

  Oral presentation


• ラット呼吸器系におけるlysozymeおよびsPLA2の分泌上皮細胞に関する免疫組織化学的研究

  増田なつみ, 万谷洋平, 西田美穂, 湯浅秀人, 河野潤一, 横山俊史, 星信彦, 北川浩

  第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

  Oral presentation


• ラットパイエル板濾胞被蓋上皮におけるToll-like receptor-2, -4および-9の発現に対する常在細菌増殖の影響

  湯浅秀人, 万谷洋平, 増田なつみ, 西田美穂, 河野潤一, 横山俊史, 星信彦, 北川浩

  第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

  Oral presentation


• ニワトリ胚における左精巣特異的な発生メカニズム

  表原拓也, 万谷洋平, 南貴一, 梅村ゆりあ, 平野哲史, 横山俊史, 北川浩, 星信彦

  日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 江別市, Domestic conference

  Oral presentation


• ニワトリ性腺の左右差形成メカニズムに関する網羅的遺伝子発現解析

  南 貴一, 表原拓也, 田渕圭章, 梅村ゆりあ, 平野哲史, 北川 浩, 横山俊史, 星 信彦

  日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 江別市, Domestic conference

  Oral presentation


• 環境ストレス下において浸透性農薬クロチアニジンが行動及び生殖機能に与える影響

  平野 哲史, 柳井 翔吾, 表原 拓也, 梅村 ゆりあ, 久保田 直人, 南 貴一, 高田 匡, 米田 直起, 広川 千英, 山本 杏, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2015, Japanese, (公社)日本獣医学会


• The short-term effect of peptidoglycan injected into the intestinal lumen against host defense responses.

  MANTANI YOUHEI, MIYAMOTO KAZUKI, MATSUMORI SEIKO, YUASA HIDETO, MASUDA NATSUMI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  17th International Congress of Mucosal Immunology, Jul. 2015, English, Berlin, Germany, International conference

  Poster presentation


• C57BL/6マウスの不可思議さ：その遺伝的背景に潜むSry-HMG box & Q-rich領域と性決定

  梅村ゆりあ, 橋本理恵, 表原拓也, 長原大知, 平野哲史, 久保田直人, 南貴一, 柳井翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第62回日本実験動物学会, May 2015, Japanese, 京都市, Domestic conference

  Poster presentation


• Sex-related genes' asymmetric expression in early mouse gonads

  梅村 ゆりあ, 橋本 理恵, 表原 拓也, 平野 哲史, 長原 大知, 湯浅 秀人, 久保田 直人, 南 貴一, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第120回 日本解剖学会総会･全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

  Poster presentation


• Selection of stable reference genes for quantitative RT-PCR analyses in developing mouse gonads

  YOKOYAMA TOSHIFUMI, 橋本 理恵, 梅村 ゆりあ, 表原 拓也, 平野 哲史, 長原 大知, 柳井 翔吾, 南 貴一, 久保田 直人, MANTANI YOUHEI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第120回 日本解剖学会総会･全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

  Poster presentation


• Reproductive and behavioral effects of clothianidin in male mice in a chronically stressed condition

  HIRANO TETSUSI, YANAI SHOUGO, OMOTEHARA TAKUYA, HASHIMOTO RIE, UMEMURA YURIA, KUBOTA NAOTO, MINAMI KIICHI, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第120回 日本解剖学会総会･全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

  Poster presentation


• Light and Electron Microscopical Study on the Particulate Bodies Found in Epithelium of Rat Airway.

  MANTANI YOUHEI, NISHIDA MIHO, YUASA HIDETO, MASUDA NATSUMI, TAKAHARA EIICHIROU, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第120回 日本解剖学会総会･全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

  Poster presentation


• Left-right asymmetry of testicular formation in the chicken embryo

  表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, 久保田 直人, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第120回 日本解剖学会総会･全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

  Poster presentation


• Differential Expression of Toll-like Receptor-2,-4 and -9 in the Various Type of Epithelia Associated with Rat Peyer’s Patches

  YUASA HIDETO, MANTANI YOUHEI, NISHIDA MIHO, TAKAHARA EI-ICHIROU, MASUDA NATSUMI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第120回 日本解剖学会総会･全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

  Poster presentation


• C57BL/6マウスの不可思議さ:その遺伝的背景に潜むSry-HMG box&Q-rich領域と性決定

  梅村ゆりあ, 橋本理恵, 表原拓也, 長原大知, 平野哲史, 久保田直人, 南貴一, 柳井翔吾, 万谷洋平, 横山俊史, 北川浩, 星信彦

  日本実験動物学会総会講演要旨集, 2015


• ネオニコチノイド系農薬クロチアニジン及び環境ストレス複合曝露が生殖器及び行動に与える影響

  平野 哲史, 柳井 翔吾, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2014, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 消化管付属リンパ組織、パイエル板の上皮におけるToll-like receptor-2、-4および-9の発現に関する免疫組織化学的研究

  湯浅 秀人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  神戸大学研究基盤センター若手フロンティア研究会2014, Dec. 2014, Japanese, 神戸市, Domestic conference

  Poster presentation


• 環境ストレス下動物の生殖機能抑制機序に関する形態機能学的研究

  平野 哲史, 柳井 翔吾, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  環境ホルモン学会第17回研究発表会, Dec. 2014, Japanese, 京都市, Domestic conference

  Oral presentation


• 発生中のマウス胎子生殖腺における内部標準遺伝子の検討

  YOKOYAMA TOSHIFUMI, 橋本 理恵, 梅村 ゆりあ, 表原 拓也, 長原 大知, 平野 哲史, 久保田 直人, 南 貴一, 湯浅 秀人, 柳井 翔吾, MANTANI YOUHEI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• 通常飼育下のラットの呼吸器系に吸入された粒子状物の粘膜上皮への取り込みに関する組織学的研究

  MANTANI YOUHEI, 西田 美穂, 湯浅 秀人, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• ラット呼吸器系粘膜上皮におけるToll-like receptor -1-9の局在に関する網羅的解析

  池邊 正大, MANTANI YOUHEI, 西田 美穂, 湯浅 秀人, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• ラットパイエル板濾胞被蓋上皮および濾胞付属腸絨毛の上皮におけるToll-like receptor-2，-4および-9の発現特性

  湯浅 秀人, MANTANI YOUHEI, 西田 美穂, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• マウス胎子生殖腺における性分化関連遺伝子発現の左右差に関する分子形態学的研究

  橋本 理恵, 梅村 ゆりあ, 表原 拓也, 平野 哲史, 長原 大知, 湯浅 秀人, 久保田 直人, 南 貴一, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬クロチアニジンの低用量曝露とストレスの複合影響

  平野 哲史, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, 柳井 翔吾, 長原 大知, 湯浅 秀人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• ニワトリ胚における性腺の形成機構とその左右差

  表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島市, Domestic conference

  Oral presentation


• ニワトリ胚における性腺の形成メカニズムと左右差

  表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, 久保田 直人, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

  Oral presentation


• 発生中のマウス胎子生殖腺における内部標準遺伝子の検討

  横山 俊史, 橋本 理恵, 梅村 ゆりあ, 表原 拓也, 長原 大知, 平野 哲史, 久保田 直人, 南 貴一, 柳井 翔吾, 万谷 洋平, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2014, Japanese, (公社)日本獣医学会


• ニワトリ胚における性腺の形成メカニズムと左右差

  表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, 久保田 直人, 柳井 翔吾, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2014, Japanese, (公社)日本獣医学会


• マウス胎子生殖腺における性分化関連遺伝子発現の左右差に関する分子形態学的研究

  橋本 理恵, 梅村 ゆりあ, 表原 拓也, 平野 哲史, 長原 大知, 湯浅 秀人, 久保田 直人, 南 貴一, 柳井 翔吾, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2014, Japanese, (公社)日本獣医学会


• ネオニコチノイド系農薬クロチアニジンの低用量曝露とストレスの複合影響

  平野 哲史, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, 柳井 翔吾, 長原 大知, 湯浅 秀人, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2014, Japanese, (公社)日本獣医学会


• 環境化学物質による内分泌かく乱研究の新展開 ネオニコチノイド系農薬による生殖機能障害とそのメカニズム

  平野 哲史, 徳本 順子, 横山 俊史, 北川 浩, 星 信彦

  日本薬学会年会要旨集, Mar. 2014, Japanese, (公社)日本薬学会


• ネオニコチノイド系農薬による生殖機能障害とそのメカニズム

  平野 哲史, 徳本 順子, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  日本薬学会第134年会, Mar. 2014, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• マウス海馬の神経新生に及ぼすTCDD毒性の時空間的影響

  小林 泰丈, 表原 拓也, 辰巳 敬哉, 橋本 理恵, 平野 哲史, 梅村 ゆりあ, 長原 大知, 横山 俊史, 北川 浩, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2013, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• セルトリ細胞の成熟に対する細胞接着の影響

  YOKOYAMA TOSHIFUMI, 金 聖民, Ferhat Ulu, 山崎 由起子

  第89回日本解剖学会近畿支部学術集会, Nov. 2013, Japanese, 奈良市, Domestic conference

  Oral presentation


• 性腺分化とその破綻メカニズムに関する分子形態学的研究

  表原 拓也, 木下 今日子, 長原 大知, 段上 めぐみ, 徳本 順子, 小林 泰丈, 辰巳 敬哉, 橋本 理恵, 平野 哲史, 梅村 ゆりあ, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ラット上行結腸の集合リンパ小節近傍の腸陰窩に出現するパネート細胞様外分泌細胞に関する超微形態学的および組織化学的研究

  MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ラット上行結腸における酸好性顆粒を有する腸陰窩上皮細胞に関する形態学的研究

  MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第89回日本解剖学会近畿支部学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ラット空腸の腸絨毛における小型乳ビ球の門脈血への移行の仕組みに関する免疫組織化学的研究

  高原 英一郎, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ラットの消化器系における補体成分C3とC4の産生部位に関する免疫組織化学的研究

  又吉 緑, MANTANI YOUHEI, 高原 英一郎, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬クロチアニジンが鳥類の繁殖能力に及ぼす影響とその作用機序

  徳本 順子, HOSHI NOBUHIKO, 橋口 峰雄, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 平野 哲史, 橋本 理恵, 梅村 ゆりあ, 長原 大知, 関島 恒夫, KAMISOYAMA HIROSHI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ウミガメの総排泄腔傍嚢の形態機能学的特徴

  辰巳 敬哉, 亀崎 直樹, AIHARA YOSHIKO, 山下 真路, 谷口 真理, 三根 佳奈子, 坂本 未来, 表原 拓也, 小林 泰丈, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, HOSHI NOBUHIKO

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• Effect of Cell-Cell contact on the maturation of Sertoli cells

  YOKOYAMA TOSHIFUMI, 金 聖民, Ferhat Ulu, 山崎 由起子

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• ウミガメの総排泄腔傍嚢の形態機能学的特徴

  辰巳敬哉, 亀崎直樹, 藍原祥子, 山下真路, 谷口真理, 三根佳奈子, 坂本未来, 表原拓也, 小林泰丈, 横山俊史, 北川浩, 今川智敬, 星信彦

  日本獣医学会学術集会講演要旨集, Aug. 2013, Japanese


• 性腺分化とその破綻メカニズムに関する分子形態学的研究

  表原 拓也, 木下 今日子, 長原 大知, 段上 めぐみ, 徳本 順子, 小林 泰丈, 辰巳 敬哉, 橋本 理恵, 平野 哲史, 梅村 ゆりあ, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2013, Japanese, (公社)日本獣医学会


• ネオニコチノイド系農薬クロチアニジンが鳥類の繁殖能力に及ぼす影響とその作用機序

  徳本 順子, 星 信彦, 橋口 峰雄, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 平野 哲史, 橋本 理恵, 梅村 ゆりあ, 長原 大知, 関島 恒夫, 上曽山 博, 横山 俊史, 北川 浩

  日本獣医学会学術集会講演要旨集, Aug. 2013, Japanese, (公社)日本獣医学会


• 環境ストレスが成熟雄マウスの生殖機能に与える影響

  平野 哲史, 長原 大知, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 橋本 理恵, 梅村 ゆりあ, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2013, Japanese, (公社)日本獣医学会


• 環境ストレス下動物の生殖機能抑制機序に関する形態機能学的研究

  平野 哲史, 小林 泰丈, 表原 拓也, 辰巳 敬哉, 橋本 理恵, 梅村 ゆりあ, 長原 大知, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第19回日本野生動物医学会大会, Aug. 2013, Japanese, 京都市, Domestic conference

  Poster presentation


• カメ類における「膀胱」の形態機能学的定義

  辰巳 敬哉, 亀崎 直樹, AIHARA YOSHIKO, 山下 真路, 谷口 真理, 三根 佳奈子, 坂本 未来, 表原 拓也, 小林 泰丈, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, HOSHI NOBUHIKO

  第19回日本野生動物医学会大会, Aug. 2013, Japanese, 京都市, Domestic conference

  Poster presentation


• Cell-Cell contact is essential for Sertoli cell maturation.

  YOKOYAMA TOSHIFUMI, KIM SUNG-MIN, ULU FERHAT, YAMAZAKI YUKIKO

  Biomedical Sciences and Health Disparities Symposium, Apr. 2013, English, Honolulu City, Hawaii,, International conference

  Poster presentation


• カメ類における「膀胱」の形態機能学的研究

  辰巳敬哉, 亀崎直樹, 藍原祥子, 山下真路, 谷口真理, 三根佳奈子, 坂本未来, 木下今日子, 横山俊史, 北川浩, 今川智敬, 金沢和樹, 星信彦

  は虫両棲類学会報, Mar. 2013, Japanese


• 生後発達期におけるTCDDの神経発達障害の分子基盤

  段上 めぐみ, 田阪 健, 長原 大知, 木下 今日子, 徳本 順子, 表原 拓也, 小林 秦丈, 辰巳 敬哉, 谷田 任司, YOKOYAMA TOSHIFUMI, 菅野 美津子, 赤星 英一, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• 生後発達期におけるTCDDによる神経発達障害の分子基盤

  段上 めぐみ, 田阪 健, 木下 今日子, 徳本 順子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 谷田 任司, 赤星 英一, 菅野 美津子, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第15回環境ホルモン学会研究発表会, Dec. 2012, Japanese, 東京，文京区, Domestic conference

  Oral presentation


• 性腺分化と性分化カスケードに関する分子形態学的研究

  木下 今日子, 長原 大知, 段上 めぐみ, 徳本 順子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• ラット上行結腸腸陰窩における常在細菌の増殖に対する生体防御に関する研究

  MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• ラット十二指腸粘膜上皮におけるMyeloid differentiation factor-2とToll-like receptor-2の発現に関する免疫組織化学的研究

  樅山 真丈, MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• ラット空腸腸絨毛の毛細血管内皮における乳ビ球に対する受容体に関する免疫組織化学的研究

  高原 英一郎, MANTANI YOUHEI, 樅山真丈, 又吉 緑, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• ラット肝臓における補体C3およびC4の産生に関する免疫組織化学的研究

  又吉 緑, MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• マウス胎子海馬の神経発達に及ぼすTCDD毒性の基礎的評価

  小林 泰丈, 段上 めぐみ, 徳本 順子, 長原 大知, 木下 今日子, 表原 拓也, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第15回環境ホルモン学会研究発表会, Dec. 2012, Japanese, 東京，文京区, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬が鳥類の繁殖能力に及ぼす影響の分子形態学的研究

  徳本 順子, 段上 めぐみ, 小林 泰丈, 木下 今日子, 表原 拓也, 辰巳 敬哉, 橋口 峰雄, 関島 恒夫, KAMISOYAMA HIROSHI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第15回環境ホルモン学会研究発表会, Dec. 2012, Japanese, 東京，文京区, Domestic conference

  Oral presentation


• ネオニコチノイド系農薬が鳥類の繁殖能力に及ぼす影響の基礎的評価

  徳本 順子, 長原 大知, 木下 今日子, 段上 めぐみ, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 橋口 峰雄, 関島 恒夫, KAMISOYAMA HIROSHI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

  Oral presentation


• カメ類における「膀胱」の形態機能学的研究

  辰巳 敬哉, 亀崎 直樹, AIHARA YOSHIKO, 山下 真路, 谷口 真理, 三根 佳奈子, 坂本 未来, 木下 今日子, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, KANAZAWA KAZUKI, HOSHI NOBUHIKO

  第51回日本爬虫類両棲類学会, Nov. 2012, Japanese, 豊田市, Domestic conference

  Oral presentation


• 遺伝子改変性転換マウス（Ypos）の真性半陰陽個体生殖腺における発生形態学的研究

  橋本 理恵, 長原 大知, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  新学術領域研究「性差構築の分子基盤」若手研究会, Oct. 2012, Japanese, 和歌山市, Domestic conference

  Oral presentation


• ニワトリ胚における性分化関連遺伝子の時空間的発現に関する分子形態学的研究

  表原 拓也, 長原 大知, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 小林 泰丈, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  新学術領域研究「性差構築の分子基盤」若手研究会, Oct. 2012, Japanese, 和歌山市, Domestic conference

  Oral presentation


• ターナーモザイク患者の性腺分化とその破綻機構の分子基盤の解明

  長原 大知, 森實 真由美, 蛯名 康彦, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 山田秀人, HOSHI NOBUHIKO

  新学術領域研究「性差構築の分子基盤」若手研究会, Oct. 2012, Japanese, 和歌山市, Domestic conference

  Oral presentation


• Y染色体腕間逆位を有するXY女性におけるSRY遺伝⼦子のエピジェネティック異常

  HOSHI NOBUHIKO, 割田(三觜) 友子, 割田 克彦, 田渕 圭章, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 櫻木 範明

  新学術領域研究「性差構築の分子基盤」若手研究会, Oct. 2012, Japanese, 和歌山市, Domestic conference

  Oral presentation


• ラット小腸腸陰窩上皮細胞の細胞死に関する超微形態学的および免疫組織化学的研究

  山本 教司, 樅山 真丈, UDAYANGA KANKANAM, GAMAGE SANATH, MANTANI YOUHEI, 高原 英一郎, 竹内 崇師, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• ラットの上行結腸における常在細菌の定着とその制御に関する定量組織学的研究

  MANTANI YOUHEI, 高原 英一郎, 竹内 崇師, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• マウス胎子の海馬におけるTCDDの神経発達毒性

  小林 泰丈, 長原 大知, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• ネオニコチノイド系殺虫剤が鳥類の繁殖能力に及ぼす影響の基礎的評価

  徳本 順子, 谷口 和美, 竹中 亜紗実, 長原 大知, 木下 今日子, 段上 めぐみ, 安田 玲子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 橋口 峰雄, 関島 恒夫, KAMISOYAMA HIROSHI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• ニワトリ胚における性分化関連遺伝子の時空間的発現に関する分子形態学的研究

  表原 拓也, 長原 大知, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 小林 泰丈, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• ターナーモザイク患者の性腺分化とその破綻機構の分子基盤の解明

  長原 大知, 森實 真由美, 蛯名 康彦, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 山田 秀人, HOSHI NOBUHIKO

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• カメ類における「膀胱」の形態機能学的解析

  辰巳 敬哉, 亀崎 直樹, 山下 真路, 長原 大知, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 小林 泰丈, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, HOSHI NOBUHIKO

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

  Oral presentation


• カメ類における「膀胱」の形態機能学的解析

  辰巳敬哉, 亀崎直樹, 山下真路, 長原大知, 木下今日子, 段上めぐみ, 徳本順子, 安田玲子, 表原拓也, 小林泰丈, 横山俊史, 北川浩, 今川智敬, 星信彦

  日本獣医学会学術集会講演要旨集, Aug. 2012, Japanese


• マウス胎子の海馬におけるTCDDの神経発達毒性作用

  小林 泰丈, 長原 大知, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 辰巳 敬哉, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2012, Japanese, (公社)日本獣医学会


• ターナーモザイク患者の性腺分化とその破綻機構の分子基盤の解明

  長原 大知, 森實 真由美, 蛯名 康彦, 木下 今日子, 段上 めぐみ, 徳本 順子, 安田 玲子, 表原 拓也, 小林 泰丈, 辰巳 敬哉, 横山 俊史, 北川 浩, 山田 秀人, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2012, Japanese, (公社)日本獣医学会


• 胎子・授乳期TCDD曝露による発達中のセロトニン神経系への影響とその作用機序

  段上 めぐみ, 須谷 真己, 石川 恭子, 藤井 裕香, 木下 今日子, 安田 玲子, 長原 大知, 谷田 任司, 横山 俊史, 北川 浩, 星 信彦

  解剖学雑誌, Jun. 2012, Japanese, (一社)日本解剖学会


• 胎子・授乳期TCDD曝露と胸腺微小環境の変化

  星 信彦, 石川 恭子, 須谷 真己, 藤井 裕香, 段上 めぐみ, 木下 今日子, 安田 玲子, 長原 大知, 谷田 任司, 横山 俊史, 北川 浩

  解剖学雑誌, Jun. 2012, Japanese, (一社)日本解剖学会


• 胎子・授乳期TCDD曝露による発達中のセロトニン神経系への影響とその作用機序

  段上 めぐみ, 須谷 真巳, 石川 恭子, 藤井 裕香, 木下 今日子, 安田 玲子, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

  Oral presentation


• 胎子・授乳期TCDD曝露と胸腺微小環境の変化

  HOSHI NOBUHIKO, 石川 恭子, 須谷 真己, 藤井 裕香, 段上 めぐみ, 木下 今日子, 安田 玲子, 長原 大知, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI

  第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

  Oral presentation


• ラットの大腸粘膜上皮におけるToll-like receptorの発現と分泌

  MANTANI YOUHEI, 亀崎 青沙, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, 谷田 任司, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

  Oral presentation


• ラットの呼吸器粘膜におけるToll-like receptorの発現と分泌

  亀崎 青沙, MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, 谷田 任司, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

  Oral presentation


• Acceleration of Epithelial Apoptosis through the Secondary Activation of Mitochondria-Dependent Pathway in the Indigenous Bacteria Settlement Sites of Rat Tongue

  UDAYANGA KANKANAM, GAMAGE SANATH, YAMAMOTO KYOJI, MANTANI YOUHEI, TAKAHARA EI-ICHIRO, KAMEZAKI AOSA, TANIDA TAKASHI, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

  Oral presentation


• Mitochondria-Dependent Pathway as a Final Apoptosis Process Secondarily Induced in Rat Esophageal Epithelium under a Physiological Condition

  UDAYANGA KANKANAM, GAMAGE SANATH, MIYATA HIDENORI, YOKOO YUH, TAKAHARA EI-ICHIRO, MANTANI YOHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  2011 Mitochondria, Apoptosis and Cancer Conference, Oct. 2011, English, Singapore, International conference

  Poster presentation


• 胎子・授乳期TCDD曝露による発達中のセロトニン神経系への影響に関する分子形態学的研究

  段上 めぐみ, 須谷 真巳, 石川 恭子, 藤井 裕香, 木下 今日子, 安田 玲子, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

  Oral presentation


• 胎子・授乳期TCDD曝露がマウス胸腺微小環境に及ぼす影響に関する分子形態学的研究

  YOKOYAMA TOSHIFUMI, 石川 恭子, 須谷 真己, 藤井 裕香, 段上 めぐみ, 木下 今日子, 安田 玲子, 谷田 任司, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

  Oral presentation


• ラット大腸におけるToll-like receptorの局在に関する免疫組織化学的研究

  MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

  Oral presentation


• ラット呼吸器系上皮におけるToll-like receptorの局在に関する免疫組織化学的研究

  亀崎 青沙, MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, 谷田 任司, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

  Oral presentation


• Acceleration of epithelial apoptosis in anterior epithelial column of filiform papilla in rat tongue by indigenous bacterial settlement.

  UDAYANGA KANKANAM, GAMAGE SANATH, YAMAMOTO KYOUJI, MANTANI YOUHEI, TAKAHARA EI-ICHIRO, TANIDA TAKASHI, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

  Oral presentation


• 胎子・授乳期TCDD曝露がマウス胸腺微小環境に及ぼす影響に関する分子形態学的研究

  横山 俊史, 石川 恭子, 須谷 真巳, 藤井 裕香, 段上 めぐみ, 木下 今日子, 安田 玲子, 谷田 任司, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2011, Japanese, (公社)日本獣医学会


• 胎子・授乳期TCDD曝露による発達中のセロトニン神経系への影響に関する分子形態学的研究

  段上 めぐみ, 須谷 真巳, 石川 恭子, 藤井 裕香, 木下 今日子, 安田 玲子, 谷田 任司, 横山 俊史, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Aug. 2011, Japanese, (公社)日本獣医学会


• 中脳発達における環境中化学物質のAhR介在性分化制御機構の解明

  段上 めぐみ, 田阪 健, 須谷 真巳, 石川 恭子, YOKOYAMA TOSHIFUMI, 赤星 英一, 菅野 美津子, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  環境ホルモン学会（日本内分泌攪乱化学物質学会）第13回研究発表会, Dec. 2010, Japanese, 東京，文京区, Domestic conference

  Poster presentation


• 逆転症系統(Ypos)由来半陰陽マウスの生殖腺における発生形態学的研究

  YOKOYAMA TOSHIFUMI, 宮本 良介, 土居 弘明, 田阪 健, 橋本 佳樹, 藤井 裕香, 石川 恭子, 須谷 真巳, 長原 大知, 三觜 友子, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第86回日本解剖学会近畿支部学術集会, Nov. 2010, Japanese, 泉佐野市, Domestic conference

  Oral presentation


• ラット消化管付属分泌腺からのToll-like receptorの分泌に関する免疫組織化学的研究

  MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第86回日本解剖学会近畿支部学術集会, Nov. 2010, Japanese, 泉佐野市, Domestic conference

  Oral presentation


• Changes in apoptosis process of rat esophageal epithelium with hyperproliferation of the indigenous bacteria under a physiological condition

  UDAYANGA KANKANAM, GAMAGE SANATH, 山本教司, 宮田英典, 横尾 諭, MANTANI YOUHEI, 高原 英一郎, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第86回日本解剖学会近畿支部学術集会, Nov. 2010, Japanese, 泉佐野市, Domestic conference

  Oral presentation


• 遺伝子改変性転換マウス(Ypos)の半陰陽個体生殖腺における発生形態学的研究

  YOKOYAMA TOSHIFUMI, 宮本 良介, 土居 弘明, 田阪 健, 橋本 佳樹, 藤井 裕香, 石川 恭子, 須谷 真巳, 長原 大知, 三觜 友子, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第150回日本獣医学会学術集会, Sep. 2010, Japanese, 帯広市, Domestic conference

  Oral presentation


• ラット消化管附属分泌腺からのToll-like receptorの分泌に関する免疫組織化学的研究

  MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第150回日本獣医学会学術集会, Sep. 2010, Japanese, 帯広市, Domestic conference

  Oral presentation


• Immunohistochemical study on the apoptotic process in normal esophageal epithelium under a physiological condition

  UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第150回日本獣医学会学術集会, Sep. 2010, Japanese, 帯広市, Domestic conference

  Oral presentation


• 遺伝子改変-性逆転マウスYposの生殖腺における発生形態学的研究

  HOSHI NOBUHIKO, 宮本 良介, YOKOYAMA TOSHIFUMI, 土居 弘明, 田阪 健, 橋本 佳樹, 藤井 裕香, 石川 恭子, 須谷 真巳, 長原 大知, 三觜 友子, KITAGAWA HIROSHI

  第50回日本先天異常学会学術集会, Jul. 2010, Japanese, 淡路市, Domestic conference

  Poster presentation


• Sry 導入XXマウスにおける雄性生殖腺の形態学的解析

  YOKOYAMA TOSHIFUMI, 土居 弘明, 宮本 良介, 田阪 健, 橋本 佳樹, 藤井 裕香, 石川 恭子, 須谷 真巳, 長原 大知, 三觜 友子, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第50回日本先天異常学会学術集会, Jul. 2010, Japanese, 淡路市, Domestic conference

  Poster presentation


• Paternal exposure to 2,3,7,8-tetrachloro- dibenzo-p-dioxin (TCDD) affects the sex ratio of offspring at birth by change the sex ratio of fertilized egg

  ISHIHARA KANA, TASAKA KEN, TANIDA TAKASHI, WARITA KATSUHIKO, MITSUHASHI TOMOKO, MIYAKE MASASHI, HARAYAMA HIROSHI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  11th International Symposium on Spermatology, Jun. 2010, English, 宜野湾市, International conference

  Poster presentation


• 短期間アルコール摂取マウス小脳の神経栄養因子リガンド/レセプター系の特異的応答性

  三木 崇範, 汪 治宇, 横山 俊史, 日下 隆, 住谷 和則, 割田 克彦, 松本 由樹, 矢倉 富子, 劉 俊騫, 王 朝暉, 竹内 義喜

  解剖学雑誌, Mar. 2010, Japanese, (一社)日本解剖学会


• ラット腸管の常在細菌と寒天平板培養細菌の超微形態学的比較検討

  伊藤 栄理, 浅田 千枝, 西田 美穂, UDAYANGA KANKANAM, GAMAGE SANATH, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• ラット腸管の上皮細胞における経時的形態学的変化とアポトーシスの進行に関する免疫組織化学的研究

  西田 美穂, 伊藤 栄理, 宮田 英典, 平野 晶子, 斉 旺梅, UDAYANGA KANKANAM, GAMAGE SANATH, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• ラット小腸腸絨毛におけるToll-like Receptorの発現

  MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 横尾 諭, 宮田英典, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• ラット小腸および大腸の粘膜リンパ組織における上皮細胞の動態の特性

  宮田 英典, 平野 晶子, 斉 旺梅, 横尾 諭, UDAYANGA KANKANAM, GAMAGE SANATH, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• マウス胎子性ライディッヒ細胞に関する発生形態学的研究

  YOKOYAMA TOSHIFUMI, 青木 香保里, 三觜 友子, 石原 可奈, 谷田 任司, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• アカネズミのAhR遺伝子多型におけるダイオキシン曝露の影響

  須谷 真巳, 石庭 寛子, 田阪 健, 藤井 裕香, 土居 弘明, 橋本 佳樹, 宮本 良介, 三觜 友子, 石原 可奈, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 関島 恒夫, HOSHI NOBUHIKO

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• TCDDによる次世代出生前性比低下と感受性との関係

  田阪 健, 石原 可奈, 土居 弘明, 橋本 佳樹, 宮本 良介, 三觜 友子, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• Sry導入XXマウスにおける雄性生殖腺の生後変化

  土居 弘明, 宮本 良介, 田阪 健, 橋本 佳樹, 三觜 友子, 石原 可奈, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• Apoptosis Process in Epidermal Epithelial Cells of Rat under a physiological condition

  UDAYANGA KANKANAM, GAMAGE SANATH, MIYATA HIDENORI, YOKOO YUH, QI WANG-MEI, NISHIDA MIHO, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

  Oral presentation


• 投与雄マウスにおける感受性と次世代出生前性比との関係

  田阪 健, 石原 可奈, 土居 弘明, 橋本 佳樹, 宮本 良介, 三觜 友子, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• 成獣雄マウスにおける性決定遺伝子Sry発現の分子学的解析

  藤井 裕香, YOKOYAMA TOSHIFUMI, 田阪 健, 須谷 真巳, MANTANI YOUHEI, 三觜 友子, 石原 可奈, 谷田 任司, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• ラット腸管の濾胞被蓋上皮における上皮細胞の動態に関する計量免疫組織化学的研究

  宮田 英典, 平野 晶子, 斉 旺梅, 横尾 諭, UDAYANGA KANKANAM, GAMAGE SANATH, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• ラット小腸の粘膜上皮におけるToll-like receptorの発現に関する免疫組織化学的研究

  MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 横尾 諭, 宮田 英典, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• ラットの腸管内腔に存在する常在細菌の超微形態学的観察

  伊藤 栄理, 浅田 千枝, 西田 美穂, UDAYANGA KANKANAM, GAMAGE SANATH, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• ラットの腸管上皮におけるアポトーシス発現に関する超微形態学的研究

  西田 美穂, 伊藤 栄理, 宮田 英典, 平野 晶子, 斉 旺梅, UDAYANGA KANKANAM, GAMAGE SANATH, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• マウスにおける胎子性ライディッヒ細胞の局在に関する発生形態学的研究

  YOKOYAMA TOSHIFUMI, 青木 香保里, 三觜 友子, 石原 可奈, 谷田 任司, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• アカネズミのAhR遺伝子多型とダイオキシン曝露の影響に関する分子形態学的評価

  須谷 真巳, 石庭 寛子, 田阪 健, 藤井 裕香, 土居 弘明, 橋本 佳樹, 宮本 良介, 三觜 友子, 石原 可奈, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 関島 恒夫, HOSHI NOBUHIKO

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• Ultrastructural Study on the Indigenous Bacteria in the Lumen of Rat Intestine.

  ITO ERI, ASADA CHIE, NISHIDA MIHO, YOKOO YUH, UDAYANGA KANKANAM, GAMAGE SANATH, QI WANG-MEI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• Ultrastructural and immunohistochemical study on the apoptotic process of intestinal epithelial cells in rats.

  NISHIDA MIHO, ITO ERI, MIYATA HIDENORI, UDAYANGA KANKANAM, GAMAGE SANATH, YOKOO YUH, QI WANG-MEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• Ultrastructural and histoplanimetrical demonstration of direct transportation of chylomicrons via subepithelial blood capillaries in rat intestinal villi.

  TAKAHARA EI-ICHIRO, ONISHI SACHIKO, NISHIDA MIHO, QI WANG-MEI, UDAYANGA KANKANAM, GAMAGE SANATH, YOKOYAMA TOSHIFUMI, WARITA KATSUHIKO, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• Sry導入XXマウスにおける雄性生殖腺の形態学的解析

  土居 弘明, 宮本 良介, 田阪 健, 橋本 佳樹, 三觜 友子, 石原 可奈, 谷田 任司, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• Scanning electron microscopic study on the persorption mechanism of large antigenic particulates from small intestinal lumen in rats.

  FUKUI YOSHITOMO, YUJI MIDORI, ONISHI SACHIKO, NISHIDA MIHO, INAMOTO TETSUROU, WARITA KATSUHIKO, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• Localization and developmental fate of fetal Leydig cells in mouse testis

  YOKOYAMA TOSHIFUMI, AOKI KAHORI, ISHIHARA KANA, MITSUHASHI TOMOKO, WARITA KATSUHIKO, HIROSHI KITAGAWA, NOBUHIKO HOSHI

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• Immunohistochemical study on the Toll-like receptor expression in villous columnar epithelial cells of rat small intestine

  MANTANI YOUHEI, YOKOO YUH, UDAYANGA KANKANAM, GAMAGE SANATH, QI WANG-MEI, NAMBA MAKIKO, NISHIDA MIHO, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• Immunohistochemical study on the apoptosis process in rat epidermis

  UDAYANGA KANKANAM, GAMAGE SANATH, MIYATA HIDENORI, YOKOO YUH, QI WANG-MEI, NISHIDA MIHO, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

  第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

  Oral presentation


• Activin A and eCG recover reproductive dysfunction induced by neonatal exposure to an estrogenic endocrine disruptor in adult male mice

  WARITA KATSUHIKO, MITSUHASHI TOMOKO, YOKOYAMA TOSHIFUMI, MATSUMOTO YOSHIKI, KITAGAWA HIROSHI, MIKI TAKANORI, TAKEUCHI YOSHIKI, HOSHI NOBUHIKO

  The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

  Poster presentation


• ラットにおけるWGA-HRPの順行性シナプス輸送の再評価(Revaluation of anterograde synaptic transport of WGA-HRP in the rat)

  竹内 義喜, 三木 崇範, 横山 俊史, 松本 由樹, 割田 克彦, 汪 治宇, 上野 貴士, 藤田 守

  解剖学雑誌, Mar. 2009, English, (一社)日本解剖学会


• 雄性化破綻メカニズムとエピジェネティクス

  三觜 友子, 割田 克彦, 菅原 照夫, 田渕 圭章, 松本 由樹, 三木 崇範, 石原 可奈, 谷田 任司, 蝦名 康彦, 櫻木 範明, 横山 俊史, 竹内 義喜, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Sep. 2008, Japanese, (公社)日本獣医学会


• 外因性エストロゲン様化学物質がLeydig細胞株TTE1のStARおよびP450scc遺伝子発現に与える影響とアセチル化ヒストンの変化

  割田 克彦, 菅原 照夫, 田渕 圭章, 三觜 友子, 松本 由樹, 三木 崇範, 石原 可奈, 谷田 任司, 横山 俊史, 竹内 義喜, 北川 浩, 星 信彦

  日本獣医学会学術集会講演要旨集, Mar. 2008, Japanese, (公社)日本獣医学会


• 外因性エストロゲン様化学物質がLeydig細胞株TTE1のステロイドホルモン産生系遺伝子発現に与える影響とヒストンアセチル化解析

  割田 克彦, 菅原 照夫, 田渕 圭章, 三觜 友子, 松本 由樹, 三木 崇範, 石原 可奈, 谷田 任司, 横山 俊史, 竹内 義喜, 北川 浩, 星 信彦

  環境ホルモン学会研究発表会要旨集, Dec. 2007, Japanese, 環境ホルモン学会(日本内分泌撹乱化学物質学会)


• 嗅球における発達中の神経系に対するイソフルレン吸入麻酔の影響

  横山 俊史, 三木 崇範, 汪 治宇, 楊 衛文, 李 京烈, 植木 正明, 竹内 義喜

  解剖学雑誌, Mar. 2007, Japanese, (一社)日本解剖学会

• 鳥類特異的な飲水調節機構の解明～進化の過程で獲得した渇きに耐えるための秘策～

  本田 和久, 横山 俊史, 佐藤 幹

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, Apr. 2024 - Mar. 2027


• 妊娠母体炎症が惹起する児の脳-身体の双方向性制御機能の破綻

  三木 崇範, 太田 健一, 鈴木 辰吾, 横山 俊史, 割田 克彦, 石川 一朗, 金西 賢治, 日下 隆, 大給 日香里

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 香川大学, 01 Apr. 2022 - 31 Mar. 2026


• Elucidation of mechanisms of generational toxicology of developmental neurotoxicity - Developmental program and epigenomic toxicity of pesticides

  星 信彦, 横山 俊史, 池中 良徳, 平野 哲史

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2022 - 31 Mar. 2025


• Elucidation of mechanisms of transgenerational effects of developmental neurotoxicity - Developmental program and epigenomic toxicity of pesticides

  星 信彦, 横山 俊史, 池中 良徳, 平野 哲史

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, Apr. 2024 - Mar. 2025


• Developmental neurotoxicity of pesticides based on the transgenerational inheritance and localized neural progenitor and glial cells activity

  星 信彦, 横山 俊史, 杉尾 翔太, 池中 良徳, 平野 哲史

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 09 Jul. 2021 - 31 Mar. 2024

  C57BL/6Nマウスに妊娠1.5日目から生後3週齢までクロチアニジン（CLO）を65 mg/kg/dayの濃度（農薬評価書における無毒性量を参考）で給水ゲルを用いて自由摂取させた．対照群または投与群同士であるF1の雌雄を交配させてF2を作製し，同様にF2の雌雄を交配させてF3を作製し，各種解析を行った．その結果，投与群F1世代の3週齢において卵巣の矮小化が認められ，それはCLOのエストロゲン関連経路活性化に起因することが示唆された．投与群F1，F2世代で抗酸化酵素GPx4の陽性強度が減少した．17OHPは，F1，F3世代10週齢では減少し，F3世代3週齢では増加した．また，世代を重ねるごとに投与群で食殺および育子放棄が増加した．F1・F2世代ではオキシトシン・プロラクチンの減少に加え，F2世代ではF1世代の不十分な養育も加重されたと推測された．網羅的遺伝子解析の結果，F1・F2世代においてCyp19a1，InhβAおよびPgrの発現が増減した．F1世代の3週齢に関しては，上記3種の遺伝子発現の増加，かつ，GPx4活性が減少したことから，CLOが酸化ストレスを誘発したことが要因と考えられた．ホルモン定量解析および遺伝子発現解析において，CLOに対する反応性は3週齢と10週齢とで異なること，ならびにCLOの胎子・授乳期曝露が雌マウスにおいて継世代影響を及ぼすことが明らかになった．また．本研究の知見に基づき，CLOのAOPを初めて明らかにした（doi: 10.1292/jvms.21-0014）．「①神経行動学的影響」及び「②二光子顕微鏡を用いた生体蛍光イメージング」については以下を参照のこと． ①doi: 10.1292/jvms.20-0721，②doi: 10.1292/jvms.22-0013，doi: 10.1038/s41598-022-09038-7


• 殺虫剤の「生物学的モニタリン グに基づく適応的リスク評価法 」の開発と実践

  星 信彦

  学術研究助成基金助成金／基盤研究(B), Apr. 2019 - Mar. 2022

  Competitive research funding


• 腸内細菌叢と脳との双方向情報伝達機構の解明−ストレスによる肥満易発症の分子基盤

  三木 崇範

  科学研究費補助金／基盤研究(C), Apr. 2018 - Mar. 2022

  Competitive research funding


• 哺乳類の未分化生殖腺の雄性化を制御するY染色体のエピジェネティック修飾の解明

  横山 俊史

  学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• 哺乳類性腺の性差構築と性特異的エピゲノム制御・維持機構の分子基盤の解明

  星 信彦

  学術研究助成基金助成金／基盤研究(C), Apr. 2016 - Mar. 2019

  Competitive research funding


• 脳が統合するエネルギー代謝調節機構−ストレスによる肥満易発症の分子機序−

  三木 崇範

  科学研究費補助金／基盤研究(C), Apr. 2014 - Mar. 2018

  Competitive research funding


• 生殖腺の性特異的分化に関わる細胞特異的エピジェネティック修飾の時空間的変化

  横山 俊史

  学術研究助成基金助成金／基盤研究(C), Apr. 2014 - Mar. 2017, Principal investigator

  Competitive research funding


• 生殖腺の性差構築とその破綻機構における細胞・組織特異的エピゲノム制御

  星 信彦

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015

  Competitive research funding


• Regulation mechanism of mammalian sex differentiation and disorders of sex development

  YOKOYAMA Toshifumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Kobe University, Apr. 2011 - Mar. 2014, Principal investigator

  We examined the relationships between regulation mechanisms of gonadal sex differentiation and disorders of sex development (DSD). In the DSD line mice (Ypos), inappropriate spatiotemporal expression of sex differentiation related factors in the developing gonad cause different phenotype from the same genetic background. In the Turner mosaic patient, the expression of sex differentiation related genes differed by gonads on both side, and the mRNA expression level was not consisting with mosaic ratio or protein expression. In addition, both three-dimensional structure and cell adherence were indispensable for the maintenance of the Sertoli cell characteristics under the culture condition. Furthermore, it became clear that GATA4 regulates expression of DMRT1 in the developing chicken gonads, and DMRT1 has various roles in the chicken embryo.

  Competitive research funding


• 周生期ストレスと生活習慣病発症機序に関する研究−脳と脂肪組織のクロストーク解析−

  三木 崇範

  科学研究費補助金／基盤研究(C), Apr. 2010 - Mar. 2014

  Competitive research funding


• Cytoarchitectural changes of brain induced by alcohol exposure : effects on Notch signal pathway

  TAKEUCHI Yoshiki, MIKI Takanori, MATSUMOTO Yoshiki, WARITA Katsuhiko, NAKAMURA Kazuhiko, IMAGAWA Tomohiro, YOKOYAMA Toshifumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kagawa University, Apr. 2006 - Mar. 2010

  Notch signaling, related with maintenance of Stemness in culture, is well known to play an important role in neuroprotective and proliferative properties. Therefore, alcohol exposure showed to activate the Notch pathway. As results, Notch signaling related receptors, such as Platelet-derived growth factor (PDGF), Brain-derived neurotrophic factor (BDNF) and Nerve growth factor (NGF), were indicated to be strongly expressed. However, there are significant lacunae about formation of neuronal network under expression of these receptors. Particularly, changes of neuronal membranes, axonal transport and synaptic transmission should be studied. In this point, a study of synaptic transport of protein might provide biological solution for psychiatric disorder of alcoholism.

  Competitive research funding


• Effects of maternal care on brain development : molecular mechanism of mother-infant interactions

  MIKI Takanori, YOKOYAMA Toshifumi, MATSUMOTO Yoshiki, WARITA Katsuhiko, TAKEUCHI Yoshiki, KUSAKA Takashi, ITOH Susumu

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kagawa University, Apr. 2006 - Mar. 2010

  Wistar male rats were separated from their mothers for 3 hours each day between PND10 and 15 (MD group). Nurotrophin ligand and receptor expressions were analyzed. The expression of BDNF/TrkB in MD rats was enhanced in both mRNA and protein levels. In contrast, no significant difference in NGF/TrkA expression level was observed between these groups. On the other hand, OMgp expression was elevated in MD animals. Abnormal fluctuations in those trophic factors cause organic changes, and ultimately lead to functional disturbance.

  Competitive research funding


• Involvement of reward system in acquisition and maintenance of alcohol independence.

  TAKEUCHI Yoshiki, MIKI Takanori, YOKOYAMA Toshihumi, WATANABE Takemi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kagawa University, Apr. 2002 - Mar. 2006

  Wistar rats were exposed to a relatively high daily dose of ethanol at postnatal day 10-15 by placing them for 3 h/day in a chamber containing ethanol vapor. These ethanol-treated rats (blood concentration : approximately 430 mg/dl) were found to have slightly but significantly fewer pyramidal cell neurons. The present observations indicate that pyramidal cells in the hippocampus may be vulnerable to a relatively high dose of ethanol exposure during this short period of early postnatal life. In additional experiment, the effects on the frontal cortex and hilar neuron numbers, observed as a result of such short periods of ethanol treatment, appeared to be transitory. With respect to colocalization of taurine and glial fibrillary acidic protein immunoreactivity in mouse hippocampus, induced by short-term ethanol exposure, quantitative analysis revealed that the merged area in ethanol-treated mice was twice that (71.6% vs. 35.8%) of control mice. Since taurine is involved in various neuroprotective functions, the present observations suggest that the expression of taurine immunoreactivity in reactive astrocytes after ethanol exposure might play an important role in neuroprotective processes. Additional analysis was made to investigate the effects on expression of neurotrophic factors. The expression levels of brain- and glia-derived neurotrophic factors (BDNF and GDNF) in the hippocampus were analyzed by RT-PCR analysis. This study revealed that chronic ethanol consumption does not result in significant changes in expression levels of BDNF and GDNF mRNAs. However, it was characteristic in myelin-associated glycoprotein that expression levels of oligodendrocyte myelin glycoprotein (OMgp) mRNA were increased. This appears to be consistent with the neuropathological changes in alcoholics, which have relationship with demyelination.

  Competitive research funding


• Effects of Maternal Deprivation on Brain Development in Rats

  MIKI Takanori, YOKOYAMA Toshihumi, KUSAKA Takashi, WATANABE Takemi, ITHO Susumu, TAKEUCHI Yoshiki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kagawa University, Apr. 2002 - Mar. 2005

  We have established the experimental animal (rat) model of maternal deprivation (MD) in human by separating pups from mothers for 3 h/day during postnatal days (PND) 10-15. Effects on body and pituitary weights The body weight of MD rats showed no significant difference at PND16, 20 and 30, whereas at PND60 it was high compared to age-matched control. On the other hand, pituitary weight was low at any time points of observation. Effects on brain weight The brain weight of MD rats showed no significant difference at any time points of observation. Corticosterone concentration During the separation period, blood corticosterone concentration of MD rats was significantly higher (51ng/dL) than that of controls (32ng/dL). After separation, it was declined to 40 ng/dL. Stereological analysis (physical disector) There were no significant difference in total number of pyramidal cells in CA1-3 regions, and granule and hilus neurons in the dentate gyrus of MD rats compared to controls. Additionally, numerical density and the regional volume of MD rats showed no differences compared to controls. Real-time PCR analysis of BDNF mRNA MD rats had less BDNF mRNA in the hippocampus at PND16, whereas at PND 30 and 60 they had much BDNF mRNA than age-matched control animals.

  Competitive research funding