Research activity information

• Dec. 2024 第16回中国地域育種談話会優秀発表賞, TILLING集団からの節水型耐乾性コムギ系統の選抜

  平田駿一郎, 山下昂太, 梅澤泰史, 贄田晃広, 辻本壽 妻鹿良亮


• Sep. 2023 第15回中国地域育種談話会優秀発表賞, パンコムギの節水型耐乾性に寄与するQTLの探索

  日名弘貴, 松岡由浩, 石井孝佳, 妻鹿良亮

  共同研究・競争的資金等の業績IDIRI


• Sep. 2023 第15回中国地域育種談話会優秀発表賞, コムギTILLING集団からの節水型耐乾性系統の選抜および生理学的解析

  平田 駿一郎, 横山 聡太郎, 辻本 壽, 妻鹿 良亮

  共同研究・競争的資金等の業績IDIRI


• Dec. 2021 中国地域育種談話会, 第13回中国地域育種談話会 優秀発表賞, カロテノイド高蓄積ネギ系統の乾燥ストレス耐性評価

  和田朋美, 執行正義, 妻鹿良亮

  Japan society


• Jan. 2020 Tottori University, Tottori University President's Award, 「節水型耐乾性」という新しいタイプの乾燥ストレス耐性を持つコムギに関する研究功績


• Dec. 2017 ムギ類研究会, 第12回ムギ類研究会ポスター賞, ABA受容体の機能強化によって創出された節水型耐乾性コムギの解析

  妻鹿良亮


• Sep. 2016 日本育種学会, 第130回講演会日本育種学会優秀発表, ABA シグナリング経路の強化によってもたらされるコムギの節水型乾燥ストレス耐性

  妻鹿良亮

• Inter-subfamily cybrid plants between wheat and maize, Zeawheat: Production via an in vitro fertilization system, genome composition, and photosynthetic type.

  Nonoka Onda, Aya Satoh, Farzana Nowroz, Tety Maryenti, Offiong Ukpong Edet, Ryosuke Mega, Takayoshi Ishii, Takashi Okamoto

  Introducing genetic resources from distantly related plants is necessary to provide novel traits to crops. However, wide hybridization between wheat and other plants is limited by the elimination of non-wheat chromosomes during the development of interspecific zygotes. In this study, we produced allopolyploid wheat-maize hybrid zygotes with various gamete combinations using an in vitro fertilization system, and monitored their developmental profiles. Hybrid zygotes produced by fusing a maize egg, a wheat egg, and a wheat sperm were successfully developed into fertile plants. Genome sequencing revealed that the regenerated plants were "cytoplasmic hybrids (cybrids)," possessing a "wheat" nuclear genome and a "wheat" + "maize" mitochondrial genome. Genomic PCR on the cybrids showed stable transmission of the maize mitochondrial genome to the F2 generation, and fluorescence in situ hybridization analysis suggested the recombination of mitochondrial DNA between wheat and maize in the cells of the cybrids. CO2 compensation point and δ13C analysis suggested that the cybrids perform C3-type photosynthesis. These results demonstrate that stable wheat cybrid lines, termed Zeawheat, can be generated by fusing heterogeneous gametes in optimal combinations. This study provides novel insights into using mitochondrial genetic resources between C3 and C4 plants.

  Aug. 2025, Journal of experimental botany, English, International magazine

  [Refereed]

  Scientific journal


• Wheat seeds exposed to heat during formation can germinate at high temperatures

  Sachiko Toda-Matsunaga, Yusuke Toda, Ryosuke Mega, Shota Tadano, Megumi Alyza, Yuji Yamasaki, Kinya Akashi, Hisashi Tsujimoto

  A capacity for reliable germination under elevated temperatures is a crucial factor in maintaining the stability of bread wheat (Triticum aestivum) yields in the context of climate change. Although the environment of the parent plant during growth is a known factor affecting seed germinability, the effect of this environment on the heat tolerance of wheat seeds has not been investigated in detail. To investigate the effect of exposure to high temperatures during growth, plants were exposed to 38°C at various growth stages. In germination test, seeds exposed to heat during their development had better heat germinability than the control. On the other hand, high temperatures before the seed development stage resulted in a lower temperature germinability compared to the control. To identify critical factors that altered heat germinability, we analyzed heat shock protein expression, fatty acid composition, and metabolite profiles. High-temperature treatment during seed formation increased the expression of heat shock proteins and reduced the degree of unsaturation of fatty acids in the seeds, which may enhance the ability of seeds to survive and germinate at high temperatures. There was a significant treatment effect on the overall metabolite content of the seeds. PLS regression analysis using the germination test results revealed that taurine, thymidine, beta-alanine, sinapic acid, and deoxyguanosine contributed significantly to germination rate. These findings suggest that the combined influence of these metabolites may play a role in acquiring seed germinability under high-temperature conditions during the growth period of the parent plants. These findings suggest potential components of a molecular mechanism in bread wheat that is triggered by high temperature during seed development and results in the acquisition of heat germinability.

  Frontiers Media SA, Mar. 2025, Frontiers in Plant Science, 16

  [Refereed]

  Scientific journal


• Effects of Drought Stress on Abscisic Acid Content and Its Related Transcripts in Allium fistulosum—A. cepa Monosomic Addition Lines

  Tetsuya Nakajima, Shigenori Yaguchi, Sho Hirata, Mostafa Abdelrahman, Tomomi Wada, Ryosuke Mega, Masayoshi Shigyo

  Jun. 2024, Genes, 15(6) (6), 754, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Metabolic profiles in drought-tolerant wheat with enhanced abscisic acid sensitivity.

  Yuanjie Weng, Ryosuke Mega, Fumitaka Abe, Hisashi Tsujimoto, Masanori Okamoto

  Global warming has led to the expansion of arid lands and more frequent droughts, which are the largest cause of global food production losses. In our previous study, we developed TaPYLox wheat overexpressing the plant hormone abscisic acid (ABA) receptor, which is important for the drought stress response in plants. TaPYLox showed resistance to drought stress and acquired water-saving traits that enable efficient grain production with less water use. In this study, we used TaPYLox to identify ABA-dependent and -independent metabolites in response to drought stress. We compared the variation of metabolites in wheat under well-watered, ABA treatment, and drought stress conditions using the ABA-sensitive TaPYLox line and control lines. The results showed that tagatose and L-serine were ABA-dependently regulated metabolites, because their stress-induced accumulation was increased by ABA treatment in TaPYLox. In contrast, L-valine, L-leucine, and DL-isoleucine, which are classified as branched chain amino acids, were not increased by ABA treatment in TaPYLox, suggesting that they are metabolites regulated in an ABA-independent manner. Interestingly, the accumulation of L-valine, L-leucine, and DL-isoleucine was suppressed in drought-tolerant TaPYLox under drought stress, suggesting that drought-tolerant wheat might be low in these amino acids. 3-dehydroshikimic acid and α-ketoglutaric acid were decreased by drought stress in an ABA-independent manner. In this study, we have succeeded in identifying metabolites that are regulated by drought stress in an ABA-dependent and -independent manner. The findings of this study should be useful for future breeding of drought-tolerant wheat.

  2024, PloS one, 19(7) (7), e0307393, English, International magazine

  [Refereed]

  Scientific journal


• Metabolomic and transcriptomic profiling during wheat seed development under progressive drought conditions

  Ryosuke Mega, June-Sik Kim, Hiroyuki Tanaka, Takayoshi Ishii, Fumitaka Abe, Masanori Okamoto

  Globally, bread wheat (Triticum aestivum) is one of the most important staple foods; when exposed to drought, wheat yields decline. Although much research has been performed to generate higher yield wheat cultivars, there have been few studies on improving end-product quality under drought stress, even though wheat is processed into flour to produce so many foods, such as bread, noodles, pancakes, cakes, and cookies. Recently, wheat cultivation has been affected by severe drought caused by global climate change. In previous studies, seed shrinkage was observed in wheat exposed to continuous drought stress during seed development. In this study, we investigated how progressive drought stress affected seed development by metabolomic and transcriptomic analyses. Metabolite profiling revealed the drought-sensitive line reduced accumulation of proline and sugar compared with the water-saving, drought-tolerant transgenic line overexpressing the abscisic acid receptor TaPYL4 under drought conditions in spikelets with developing seeds. Meanwhile, the expressions of genes involved in translation, starch biosynthesis, and proline and arginine biosynthesis was downregulated in the drought-sensitive line. These findings suggest that seed shrinkage, exemplifying a deficiency in endosperm, arose from the hindered biosynthesis of crucial components including seed storage proteins, starch, amino acids, and sugars, ultimately leading to their inadequate accumulation within spikelets. Water-saving drought tolerant traits of wheat would aid in supporting seed formation under drought conditions.

  Sep. 2023, Scientific Reports, 13(1) (1), 15001, English, International magazine

  [Refereed]

  Scientific journal


• Author Correction: Tuning water-use efficiency and drought tolerance in wheat using abscisic acid receptors

  Ryosuke Mega, Fumitaka Abe, June-Sik Kim, Yuuri Tsuboi, Keisuke Tanaka, Hisato Kobayashi, Yoichi Sakata, Kousuke Hanada, Hisashi Tsujimoto, Jun Kikuchi, Sean R. Cutler, Masanori Okamoto

  Apr. 2023, Nature Plants, 9(6) (6), 1001 - 1001, English, International magazine

  Scientific journal


• Metabolome Profiling of Heat Priming Effects, Senescence, and Acclimation of Bread Wheat Induced by High Temperatures at Different Growth Stages.

  Sachiko Matsunaga, Yuji Yamasaki, Ryosuke Mega, Yusuke Toda, Kinya Akashi, Hisashi Tsujimoto

  Our previous study described stage-specific responses of 'Norin 61' bread wheat to high temperatures from seedling to tillering (GS1), tillering to flowering (GS2), flowering to full maturity stage (GS3), and seedling to full maturity stage (GS1-3). The grain development phase lengthened in GS1 plants; source tissue decreased in GS2 plants; rapid senescence occurred in GS3 plants; all these effects occurred in GS1-3 plants. The present study quantified 69 flag leaf metabolites during early grain development to reveal the effects of stage-specific high-temperature stress and identify markers that predict grain weight. Heat stresses during GS2 and GS3 showed the largest shifts in metabolite contents compared with the control, followed by GS1-3 and GS1. The GS3 plants accumulated nucleosides related to the nucleotide salvage pathway, beta-alanine, and serotonin. Accumulation of these compounds in GS1 plants was significantly lower than in the control, suggesting that the reduction related to the high-temperature priming effect observed in the phenotype (i.e., inhibition of senescence). The GS2 plants accumulated a large quantity of free amino acids, indicating residual effects of the previous high-temperature treatment and recovery from stress. However, levels in GS1-3 plants tended to be close to those in the control, indicating an acclimation response. Beta-alanine, serotonin, tryptophan, proline, and putrescine are potential molecular markers that predict grain weight due to their correlation with agronomic traits.

  Dec. 2021, International journal of molecular sciences, 22(23) (23), English, International magazine

  [Refereed]

  Scientific journal


• Genomic analysis for heat and combined heat–drought resilience in bread wheat under field conditions

  Michael O. Itam, Ryosuke Mega, Yasir S. A. Gorafi, Yuji Yamasaki, Izzat S. A. Tahir, Kinya Akashi, Hisashi Tsujimoto

  Oct. 2021, Theoretical and Applied Genetics, 135, 337 - 350, English

  [Refereed]

  Scientific journal


• Stage-Specific Characterization of Physiological Response to Heat Stress in the Wheat Cultivar Norin 61

  Sachiko Matsunaga, Yuji Yamasaki, Yusuke Toda, Ryosuke Mega, Kinya Akashi, Hisashi Tsujimoto

  Bread wheat (Triticum aestivum) is less adaptable to high temperatures than other major cereals. Previous studies of the effects of high temperature on wheat focused on the reproductive stage. There are few reports on yield after high temperatures at other growth stages. Understanding growth-stage-specific responses to heat stress will contribute to the development of tolerant lines suited to high temperatures at various stages. We exposed wheat cultivar “Norin 61” to high temperature at three growth stages: seedling–tillering (GS1), tillering–flowering (GS2), and flowering–maturity (GS3). We compared each condition based on agronomical traits, seed maturity, and photosynthesis results. Heat at GS2 reduced plant height and number of grains, and heat at GS3 reduced the grain formation period and grain weight. However, heat at GS1 reduced senescence and prolonged grain formation, increasing grain weight without reducing yield. These data provide fundamental insights into the biochemical and molecular adaptations of bread wheat to high-temperature stresses and have implications for the development of wheat lines that can respond to high temperatures at various times of the year.

  {MDPI} {AG}, Jun. 2021, International Journal of Molecular Sciences, 22(13) (13), 6942 - 6942

  [Refereed]

  Scientific journal


• Functional enhancement of abscisic acid receptor confers water-saving and drought tolerance in wheat

  Ryosuke Mega, Masanori Okamoto

  Lead, Nov. 2020, Regulation of Plant Growth & Development, 55(2) (2), 126 - 130, Japanese

  [Refereed][Invited]


• Aegilops tauschii Introgressions Improve Physio-Biochemical Traits and Metabolite Plasticity in Bread Wheat under Drought Stress

  Michael Itam, Mostafa Abdelrahman, Yuji Yamasaki, Ryosuke Mega, Yasir Gorafi, Kinya Akashi, Hisashi Tsujimoto

  With the aim of providing genetic materials for breeding drought-resistant wheat varieties, the physiological and metabolic plasticity of three drought-resistant wheat multiple synthetic derivative lines (MSDLs) and their backcross parent “Norin 61” (N61) were evaluated in response to drought stress. The results indicated that the D-genome introgressions from Aegilops tauschii into the MDSLs improved their drought-adaptive traits. Specifically, MNH5 and MSD345 showed higher photosynthesis rates and triose phosphate utilization than N61 under control conditions, resulting in greater accumulation of glucose and sucrose in the shoots. However, under drought stress, MNH5 and MSD345 had higher intrinsic water use efficiency than MSD53 and N61. The total antioxidant capacity and superoxide dismutase activity increased in all three MSDLs, whereas no significant changes were found in N61 in response to drought stress. Metabolome analysis identified six common drought-induced metabolites in all of the investigated genotypes. However, four metabolites (adenine, gamma aminobutyric acid, histidine, and putrescine) each specifically accumulated in an MSDL in response to drought stress, suggesting that these metabolites are important for MSDL drought resistance. In conclusion, MNH5 and MSD345 showed better adaptive responses to drought stress than MSD53 and N61, suggesting that these two MSDLs could be explored for breeding drought-resistant wheat lines.

  {MDPI} {AG}, Oct. 2020, Agronomy, 10(10) (10), 1588 - 1588

  [Refereed]

  Scientific journal


• Metabolic and physiological responses to progressive drought stress in bread wheat.

  Michael Itam, Ryosuke Mega, Shota Tadano, Mostafa Abdelrahman, Sachiko Matsunaga, Yuji Yamasaki, Kinya Akashi, Hisashi Tsujimoto

  Wheat (Tritium aestivum) is vulnerable to future climate change because it is predominantly grown under rain-fed conditions in drought-prone areas. Thus, in-depth understanding of drought effect on wheat metabolism is essential for developing drought-tolerant wheat varieties. Here, we exposed wheat 'Norin 61' plants to progressive drought stress [0 (before drought), 2, 4, 6, 8, and 10 days after withholding water] during the flowering stage to investigate physiological and metabolomic responses. Transcriptional analyses of key abscisic acid-responsive genes indicated that abscisic acid signalling played a major role in the adaptation of wheat to water deficit. Carbon isotope composition had a higher value than the control while canopy temperature (CT) increased under drought stress. The CT depression was tightly correlated with soil water potential (SWP). Additionally, SWP at - 517 kPa was identified as the critical point for increasing CT and inducing reactive oxygen species. Metabolome analysis identified four potential drought-responsive biomarkers, the enhancement of nitrogen recycling through purine and pyrimidine metabolism, drought-induced senescence based on 1-aminocyclopropane-1-carboxylic acid and Asn accumulation, and an anti-senescence response through serotonin accumulation under severe drought stress. Our findings provide in-depth insight into molecular, physiological and metabolite changes involved in drought response which are useful for wheat breeding programs to develop drought-tolerant wheat varieties.

  Corresponding, Oct. 2020, Scientific reports, 10(1) (1), 17189 - 17189, English, International magazine

  [Refereed]

  Scientific journal


• The crystal structures of Thermus thermophilus CMP kinase complexed with a phosphoryl group acceptor and donor

  Ryosuke Mega, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui

  Lead, Public Library of Science (PLoS), May 2020, PLOS ONE, 15(5) (5), e0233689 - e0233689, English

  [Refereed]

  Scientific journal


• Dynamic control of plant water use using designed ABA receptor agonists

  Aditya S. Vaidya, Jonathan D. M. Helander, Francis C. Peterson, Dezi Elzinga, Wim Dejonghe, Amita Kaundal, Sang-Youl Park, Zenan Xing, Ryousuke Mega, Jun Takeuchi, Bardia Khanderahoo, Steven Bishay, Brian F. Volkman, Yasushi Todoroki, Masanori Okamoto, Sean R. Cutler

  American Association for the Advancement of Science ({AAAS}), Oct. 2019, Science, 366(6464) (6464), eaaw8848, English

  [Refereed]

  Scientific journal


• Genetic manipulation of abscisic acid receptors enables modulation of water use efficiency

  Ryosuke Mega, Hisashi Tsujimoto, Masanori Okamoto

  Lead, Oct. 2019, PLANT SIGNALING & BEHAVIOR, 14(10) (10), e1642039, English

  [Refereed]

  Scientific journal


• Tuning water-use efficiency and drought tolerance in wheat using abscisic acid receptors

  Ryosuke Mega, Fumitaka Abe, June-Sik Kim, Yuuri Tsuboi, Keisuke Tanaka, Hisato Kobayashi, Yoichi Sakata, Kousuke Hanada, Hisashi Tsujimoto, Jun Kikuchi, Sean R. Cutler, Masanori Okamoto

  Lead, Feb. 2019, NATURE PLANTS, 5(2) (2), 153 - +, English

  [Refereed]

  Scientific journal


• Physiological Response of Wheat to Chemical Desiccants Used to Simulate Post-Anthesis Drought Stress

  Nasrein Mohamed Kamal, Yasir Serag Alnor Gorafi, Ryosuke Mega, Hisashi Tsujimoto

  Apr. 2018, AGRONOMY-BASEL, 8(4) (4), 44, English

  [Refereed]

  Scientific journal


• Abscinazole-E3M, a practical inhibitor of abscisic acid 8 '-hydroxylase for improving drought tolerance

  Jun Takeuchi, Masanori Okamoto, Ryosuke Mega, Yuri Kanno, Toshiyuki Ohnishi, Mitsunori Seo, Yasushi Todoroki

  Nov. 2016, SCIENTIFIC REPORTS, 6, 37060, English

  [Refereed]

  Scientific journal


• Physiological action and application of abscisic acid, which controls drought stress response in plants

  岡本 昌憲, 妻鹿 良亮

  [極限環境生物学会] 学会事務局, Dec. 2015, Journal of Japanese Society for Extremophiles, 14(2) (2), 78 - 88, Japanese

  [Refereed][Invited]


• Sustained low abscisic acid levels increase seedling vigor under cold stress in rice (Oryza sativa L.)

  Ryosuke Mega, Ayano Meguro-Maoka, Akira Endo, Etsuo Shimosaka, Seiji Murayama, Eiji Nambara, Mitsunori Seo, Yuri Kanno, Suzanne R. Abrams, Yutaka Sato

  Lead, Sep. 2015, SCIENTIFIC REPORTS, 5, 13819, English

  [Refereed]

  Scientific journal


• A non-cold-inducible cold shock protein homolog mainly contributes to translational control under optimal growth conditions

  Toshiko Tanaka, Ryosuke Mega, Kwang Kim, Akeo Shinkai, Ryoji Masui, Seiki Kuramitsu, Noriko Nakagawa

  Mar. 2012, FEBS JOURNAL, 279(6) (6), 1014 - 1029, English

  [Refereed]

  Scientific journal


• An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response

  Rihito Morita, Hisahiro Hishinuma, Hiromasa Ohyama, Ryosuke Mega, Toshihiro Ohta, Noriko Nakagawa, Yoshihiro Agari, Kenji Fukui, Akeo Shinkai, Seiki Kuramitsu, Ryoji Masui

  Sep. 2011, JOURNAL OF BIOCHEMISTRY, 150(3) (3), 327 - 339, English

  [Refereed]

  Scientific journal


• Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8

  Mayumi Kanagawa, Seiki Baba, Akio Ebihara, Akeo Shinkai, Ken Hirotsu, Ryosuke Mega, Kwang Kim, Seiki Kuramitsu, Gen-ichi Sampei, Gota Kawai

  Aug. 2010, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 66(8) (8), 893 - 898, English

  [Refereed]

  Scientific journal


• Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus

  Ryosuke Mega, Miho Manzoku, Akeo Shinkai, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui

  Lead, Aug. 2010, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 399(3) (3), 336 - 340, English

  [Refereed]

  Scientific journal


• Two dNTP triphosphohydrolases from Pseudomonas aeruginosa possess diverse substrate specificities

  Ryosuke Mega, Naoyuki Kondo, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui

  Lead, Jun. 2009, FEBS JOURNAL, 276(12) (12), 3211 - 3221, English

  [Refereed]

  Scientific journal


• Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin

  Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Takuma Oda, Koichi Hirata, Hisashi Nishiwaki, Takako Morimoto, Miki Akamatsu, Yuji Ashikawa, Shun'Ichi Kuroda, Ryosuke Mega, Seiki Kuramitsu, David B. Sattelle, Kazuhiko Matsuda

  Jun. 2008, Invertebrate Neuroscience, 8(2) (2), 71 - 81, English

  [Refereed]

  Scientific journal

• Phosphoproteome analysis between water-saving and non-water-saving wheat

  平田駿一郎, 山下昂太, 梅澤泰史, 贄田晃広, 辻本壽, 妻鹿良亮

  Corresponding, 2024, 育種学研究, 26


• Screening of QTL contributing to water-saving drought-tolerance using wheat RIL population

  日名弘貴, 石井孝佳, 西村和紗, 松岡由浩, 辻本壽, 妻鹿良亮

  2024, 育種学研究, 26


• ハイパースペクトルデータとドローン空撮データを用いた植物体の含水率モニタリング手法の検討

  濱侃, 小大塚生登, 木村蒼, 松橋寛太, 松岡延浩, 木村玲二, 石井孝佳, 妻鹿良亮

  2024, 日本農業気象学会全国大会講演要旨(CD-ROM)


• Molecular and physiological analyses of abscisic acid receptor-mediated disease resistance in wheat

  WENG Yuanjie, WENG Yuanjie, 嶋崎太一, 妻鹿良亮, 安倍史高, KIM J., 吉田健太郎, 二瓶賢一, 岡本昌憲, 岡本昌憲

  2024, 育種学研究, 26


• Seedling morphology of wheat associated with cytokinin and auxin levels due to high temperature stress

  松永幸子, 山崎裕司, 金俊植, 辻本壽, 妻鹿良亮

  2023, 植物の生長調節, 58(Supplement) (Supplement)


• Selection and physiological analysis of water-saving drought-tolerant genotypes from the wheat TILLING population

  平田駿一郎, 横山聡太郎, 辻本壽, 妻鹿良亮

  2023, 育種学研究, 25


• Metabolite analysis and development for biomarker-based breeding of wheat

  妻鹿良亮

  Lead, Jul. 2022, アグリバイオ, 6(7) (7), 70 - 73


• Molecular elucidation and application for water-saving of wheat

  MEGA Ryosuke

  Lead, May 2022, アグリバイオ, 6(5) (5), 62 - 65, Japanese

  Introduction scientific journal


• Comprehensive analysis of metabolite changes in response to drought stress in wheat

  WENG Yuanjie, 金俊植, 妻鹿良亮, 辻本壽, 岡本昌憲, 岡本昌憲, 岡本昌憲

  2022, 植物の生長調節, 57(Supplement) (Supplement)


• アブシシン酸受容体がもたらすコムギうどんこ病抵抗性機構の分子解析

  嶋崎太一, 金俊植, 妻鹿良亮, 安倍史高, 宮本皓司, 二瓶賢一, 吉田健太郎, 岡本昌憲, 岡本昌憲, 岡本昌憲

  2022, 日本植物学会大会研究発表記録(CD-ROM), 86th


• Screening of water-saving wheat genotypes from natural mutation populations

  妻鹿良亮, 山崎裕司, 辻本壽

  2022, 育種学研究, 24


• Comprehensive analysis of metabolites in response to drought stress in wheat

  WENG Yuanjie, KIM J., 妻鹿良亮, 辻本壽, 岡本昌憲

  2022, 育種学研究, 24


• Molecular analysis of abscisic acid receptor-mediated powdery mildew resistant mechanism in wheat

  嶋崎太一, KIM J., 妻鹿良亮, 安倍史高, 宮本皓司, 山根久和, 二瓶賢一, 吉田健太郎, 岡本昌憲

  2022, 育種学研究, 24


• Physiological analysis of Arabidopsis mutant that lost all members of ABA 8’-hydroxylase

  宮田百華, 妻鹿良亮, KIM June-Sik, 瀬尾光範, 南原英司, 岡本昌憲

  2022, 日本植物生理学会年会(Web), 63rd


• Comprehensive analysis of gene expression and metabolite changes in response to drought stress in wheat

  WENG Yuanjie, KIM June-Sik, 妻鹿良亮, 辻本壽, 岡本昌憲

  2022, 日本植物生理学会年会(Web), 63rd


• Molecular analysis of abscisic acid receptor-mediated blight resistant mechanism in wheat

  嶋崎太一, 金俊植, 妻鹿良亮, 安倍史高, 宮本皓司, 山根久和, 吉田健太郎, 岡本昌憲

  2021, 植物の生長調節, 56(Supplement) (Supplement)


• A multi-omics study on heat response by different growth-stages toward tolerant bread wheat selection

  松永幸子, 戸田悠介, 妻鹿良亮, 山崎裕司, 辻本壽, 辻本壽

  2021, 育種学研究, 23


• Canopy temperature, Ionome and carbon isotope ratio would reveal physiological perspective of high yield wheat cultivar in Hokkaido

  大西志全, 神谷岳洋, 妻鹿良亮, 妻鹿良亮, 藤原徹, 辻本壽, 佐藤優美, 森田耕一, 森田耕一

  2021, 育種学研究, 23


• Growth stage-specific heat stress response in wheat

  松永幸子, 山崎裕司, 妻鹿良亮, 辻本壽

  2020, 育種学研究, 22


• Effects of pathogenic infection on lipid metabolism in barley

  松原正幸, 大賀萌, 宇部尚樹, 妻鹿良亮, 辻本壽, 上野琴巳, 石原亨

  2020, 日本農薬学会大会講演要旨集, 45th


• 干ばつに強い節水型コムギ

  Mar. 2019, 科学新聞


• 乾燥に強いコムギ開発 宇都宮大など、少ない水で光合成

  Mar. 2019, 日本経済新聞

  Others


• 宇都宮大ら、節水型耐乾性コムギの開発に成功

  Feb. 2019, 米麦日報


• 乾燥に強い節水コムギ

  Feb. 2019, 日本海新聞, Japanese

  Others


• 水不足にも負けぬ小麦

  Feb. 2019, 朝日新聞, Japanese

  Others


• シロイヌナズナにおけるアブシシン酸不活性化鍵酵素の機能解析

  宮田百華, 妻鹿良亮, KIM June-Sik, 瀬尾光範, 南原英司, 岡本昌憲

  2019, 植物の生長調節, 54(Supplement) (Supplement)


• アブシシン酸受容体がもたらすコムギ病害抵抗性機構の解析

  嶋崎太一, KIM June-Sik, 妻鹿良亮, 安倍史高, 山根久和, 吉田健太郎, 岡本昌憲

  2019, 植物の生長調節, 54(Supplement) (Supplement)


• 高温ストレス耐性コムギ育成に向けた代謝物プロファイリング

  松永幸子, 山崎裕司, 妻鹿良亮, 辻本壽

  2019, 育種学研究, 21


• 節水型耐乾性コムギは乾燥ストレスによる種子品質低下を緩和する

  妻鹿良亮, 石井孝佳, 安倍史高, 菊地淳, 坪井裕理, 坪井裕理, 田中裕之, 岡本昌憲, 辻本壽

  2019, 育種学研究, 21


• コムギのアブシシン酸受容体過剰発現による光合成速度の促進

  妻鹿良亮, 安倍史高, KIM June-sik, 田中啓介, 小林久人, 坂田洋一, 辻本壽, 岡本昌憲

  2019, 植物の生長調節, 54(Supplement) (Supplement)


• 限界地でコムギ栽培夢見て

  妻鹿良亮

  2017, 朝日新聞「砂丘から世界へ⑧」, Japanese

  Introduction commerce magazine


• 節水型耐乾性を獲得したコムギは限られた水でも種子の品質を維持する

  妻鹿良亮, 安倍史高, 菊地淳, KIM J., 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲, 岡本昌憲, 岡本昌憲

  2017, 育種学研究, 19


• ABAシグナリング経路の強化によってもたらされるコムギの節水型乾燥ストレス耐性

  妻鹿良亮, 安倍史高, KIM J., 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲, 岡本昌憲

  2016, 育種学研究, 18


• アブシジン酸受容体の強化はコムギの節水型耐乾性の形質を付与する

  妻鹿良亮, 安倍史高, KIM June-sik, 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲, 岡本昌憲

  2016, 植物の生長調節, 51(Supplement) (Supplement)


• ABAシグナリング経路の強化によってもたらされるコムギの節水型乾燥ストレス耐性

  妻鹿良亮, 安倍史高, KIM J., 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲, 岡本昌憲

  2016, 育種学研究, 18


• P106 Functional analysis of abscinazole-E3M, a novel inhibitor of ABA 8'-hydroxylases

  Masuda Junya, Mega Ryosuke, Okamoto Masanori, Todoroki Yasushi

  The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2015, 植物化学調節学会研究発表記録集, 50, 124 - 124, Japanese


• P102 Improvement of wheat drought tolerance utilizing ABA receptor

  Mega Ryosuke, Abe Fumitaka, Hanada Kousuke, Okamoto Masanori

  The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2015, 植物化学調節学会研究発表記録集, 50, 120 - 120, Japanese


• アブシジン酸分解酵素遺伝子によるイネ幼苗の低温伸長性の改良

  佐藤裕, 妻鹿良亮, 目黒文乃, 遠藤亮, 下坂悦生, 村山誠治, 南原英司, 瀬尾光範, 菅野裕理, ABRAMS Suzanne R.

  2015, 農研機構北海道農業研究センター成果情報(Web), 2015


• 25. Identification and biochemical characterization of wheat ABA receptors, which are identified by bioinformatics analysis

  Mega Ryosuke, Hanada Kousuke, Okamoto Masanori

  The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2014, 植物化学調節学会研究発表記録集, 49, 43 - 43, Japanese


• 高度好熱菌のリン酸化プロテオーム解析:立体構造に基づくリン酸化部位のマッピング

  高畑良雄, 井上真男, 飯尾洋太, 妻鹿良亮, 高橋裕佳, 友池史明, 中川紀子, KIM Kwang, 宮本昌明, 石濱泰, 増井良治, 倉光成紀

  31 May 2012, 日本蛋白質科学会年会プログラム・要旨集, 12th, 138, Japanese


• 真正細菌におけるdNTP triphosphohydrolaseの多様性

  妻鹿良亮, 近藤直幸, 近藤直幸, 中川紀子, 中川紀子, 倉光成紀, 倉光成紀, 増井良治, 増井良治

  2007, 高度好熱菌丸ごと一匹プロジェクト 第6回連携研究会 理研シンポジウム 平成19年


• Thermus thermophilus HB8タンパク質の機能発見研究:dNTP triphosphohydrolaseの生体内におけるdNTP調節機構に対する寄与

  妻鹿良亮, 近藤直幸, 近藤直幸, 中川紀子, 中川紀子, 倉光成紀, 倉光成紀, 倉光成紀, 増井良治, 増井良治

  2007, 生化学


• 高度好熱菌Thermus thermophilus HB8由来dNTP triphosphohydrolaseのドメインおよびサブユニット解析

  妻鹿良亮, 近藤直幸, 中川紀子, 中川紀子, 増井良治, 増井良治, 倉光成紀, 倉光成紀

  2006, 高度好熱菌丸ごと一匹プロジェクト 第5回連携研究会 理研シンポジウム 平成18年


• 高度好熱菌dNTP triphosphohydeolaseのドメイン解析

  妻鹿良亮, 近藤直幸, 中川紀子, 中川紀子, 増井良治, 増井良治, 倉光成紀, 倉光成紀

  2005, 日本分子生物学会年会講演要旨集, 28th

• 節水型乾燥ストレス耐性コムギの分子生理学研究

  国立大学法人九州大学 実験生物環境制御センター 2024年シンポジウム, Jul. 2024, Japanese

  [Invited]

  Nominated symposium


• ハイパースペクトルデータとドローン空撮データを用いた植物体の含水率モニタリング手法の検討

  濱侃, 小大塚生登, 木村蒼, 松橋寛太, 松岡延浩, 木村玲二, 石井孝佳, 妻鹿良亮

  日本農業気象学会2024年全国大会, Mar. 2024, Japanese

  Oral presentation


• METABOLOMIC AND TRANSCRIPTOMIC APPROACH TO DETERMINE PLANT HEAT TOLERANT MECHANISM

  Ryosuke Mega

  Biotech Meets AI: Transforming Agriculture through Innovative Technologies, Priority University 2023, Jan. 2024, English

  [Invited]

  Invited oral presentation


• Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions

  Ryosuke Mega

  第18回ムギ類研究会, Dec. 2023, Japanese

  [Invited]

  Invited oral presentation


• Seedling morphology of wheat associated with cytokinin and auxin levels due to high temperature stress

  松永幸子, 山崎裕司, 金俊植, 辻本壽, 妻鹿良亮

  植物化学調節学会第58回大会, Nov. 2023, Japanese

  Poster presentation


• Analysis of molecular effects in developing seeds by drought stress

  R. Mega, T. Ishii, H. Tanaka, F. Abe, M. Okamoto

  日本育種学会第142回講演会, Sep. 2022, Japanese

  Oral presentation


• Analysis of molecular effects in developing seeds by drought stress

  Ryosuke Mega, Hiroyuki Tanaka, June-Sik Kim, Takayoshi Ishii, Fumitaka Abe, Masanori Okamoto

  2nd International Wheat Congress, English

  Poster presentation


• 節水型耐乾性コムギは乾燥ストレスによる種子品質低下を緩和する

  妻鹿良亮, 石井孝佳, 安倍史高, 菊地淳, 坪井裕理, 田中裕之, 岡本昌憲, 辻本壽

  日本育種学会第136回講演会, Sep. 2019, Japanese


• Water-saving wheat: tuning water use efficiency and drought tolerance using ABA receptors.

  Ryosuke Mega, Fumitaka Abe, June-sik Kim, Hisashi Tsujimoto, Jun Kikuchi, Masanori Okamoto

  IWC 1st International Wheat Congress, Jul. 2019, English

  Poster presentation


• Water-saving wheat: tuning water use efficiency and drought tolerance using ABA receptors.

  Mega, R

  13TH INTERNATIONAL CONFERENCE ON DEVELOPMENT OF DRYLANDS, Feb. 2019, English

  Invited oral presentation


• ABA受容体の機能強化によって創出された節水型耐乾性コムギの解析

  妻鹿 良亮

  第10回中国地域育種談話会, Dec. 2018, Japanese

  Poster presentation


• ABA受容体の機能強化によって創出された節水型耐乾性コムギの解析

  妻鹿 良亮

  平成30年度 共同研究発表会, Dec. 2018, Japanese

  Poster presentation


• 節水型耐乾性コムギのMSD集団からの選抜

  妻鹿 良亮

  第13回ムギ類研究会, Nov. 2018, Japanese

  Poster presentation


• ABA受容体の機能強化によって創出された節水型耐乾性コムギの解析

  妻鹿 良亮

  戦略的創造研究推進事業（さきがけ） 研究領域「情報科学との協働による革新的な農産物栽培手法を実現するための技術基盤の創出」 第６回領域会議, Aug. 2018, Japanese

  Invited oral presentation


• Water-saving wheat: tuning water use efficiency and drought tolerance using ABA receptors

  Ryosuke Mega

  ASPB2018, Jul. 2018, English, International conference

  Oral presentation


• ABA受容体機能の強化により獲得した節水型耐乾性コムギの生理機能解析

  妻鹿良亮

  第21回穂発芽研究会（つくば）, Dec. 2017, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 節水型耐乾性を獲得したコムギは限られた水でも種子の品質を維持する

  妻鹿良亮, 安倍史高, 菊地淳, KIM J, 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲

  育種学研究, Oct. 2017, Japanese

  Oral presentation


• Enhancement of ABA receptor function confers water-saving drought tolerance in wheat.

  Ryosuke Mega

  2017 IPSR International Forum on Plant Stress Science for Young Researchers, Aug. 2017, English, International conference

  [Invited]

  Invited oral presentation


• ABAシグナリング経路の強化によってもたらされるコムギの節水型乾燥ストレス耐性

  妻鹿良亮

  第11回ムギ類研究会・第8回中国地域育種談話会 (共催), Dec. 2016, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• アブシジン酸受容体の強化はコムギの節水型耐乾性の形質を付与する

  妻鹿良亮, 安倍史高, KIM June‐sik, 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲, 岡本昌憲

  植物の生長調節, Oct. 2016, Japanese


• ABAシグナリング経路の強化によってもたらされるコムギの節水型乾燥ストレス耐性

  妻鹿良亮, 安倍史高, KIM J, 田中啓介, 小林久人, 坂田洋一, 辻本壽, 花田耕介, 岡本昌憲

  育種学研究, Sep. 2016, Japanese


• Water-saving drought stress tolerance of wheat due to enhancement of ABA signaling pathway.

  Mega, R

  TWELFTH INTERNATIONAL CONFERENCE ON DEVELOPMENT OF DRYLANDS, Aug. 2016, English

  Poster presentation


• ABA受容体を利用したコムギの乾燥ストレス耐性の向上

  妻鹿良亮, 安倍史高, 花田耕介, 岡本昌憲

  植物の生長調節, Oct. 2015, Japanese

• American Society of Plant Biologists


• THE JAPANESE SOCIETY FOR CHEMICAL REGULATION OF PLANTS


• JAPANESE SOCIETY OF BREEDING


• 日本食品科学工学会

• 香気成分に注目したコムギの品種開発に向けた分析技術の確立と系統選抜

  公益財団法人エリザベス・アーノルド富士財団, 学術研究助成金, 神戸大学, Aug. 2025 - Jul. 2026, Principal investigator


• 干ばつ下でも高品質の小麦粉が生産可能な品種開発に向けた基礎的研究

  公益財団法人エリザベス・アーノルド富士財団, 学術研究助成金, 山口大学, Aug. 2023 - Jul. 2024, Principal investigator


• Multi-omics study for targeting genes related to heat tolerance and functionality in leaf bunching onion cultivar

  執行 正義, 藤井 宏栄, 名田 和義, 佐藤 修正, 平井 優美, 妻鹿 良亮

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Yamaguchi University, Apr. 2021 - Mar. 2024, Coinvestigator

  濃緑色個体と淡緑色個体間の交雑集団を用いた連鎖地図の構築に関しては、先ず、極濃緑色の山口県オリジナル品種「YSG1号」(P1)と緑色の市販品種の「浅黄系九条」(P2)を親素材とし、これらの正逆F1とF2集団を作出した。次に、遺伝子型の情報を特定のF2 集団90個体で整理し、連鎖地図の作成や表現型とのアソシエーションを解析するための基盤情報として整備した。SPAD値で評価した葉色表現型の上位50系統、下位50系統を選抜し、両親系統と合わせてRNA-seq解析を行なった。1サンプルあたり平均で2,800万リード得られた配列情報を、先行プロジェクトで収集していたネギのunigene配列（68,726種）をリファレンスとしてマッピングすることにより、親系統の“YSG1”と”九条”の間で多型が認められるサイトの抽出を実施した。 葉色関連形質の表現型値の取得については、草丈が40～50㎝のサイズに生育した時期に葉身色を調査した。葉身色はSPADと色彩色差計（L*、a*、b*）で測定し、若い葉身から1枚目と2枚目の中央部の平均値をネギ個体の葉身色とした。また、ジェノタイピング集団に関しては、クロロフィル・カロテノイド類含量の測定を実施した。自然免疫活性化に関しては、関連タンパクの発現解析に用いる抗体作成を行うとともに、葉ネギにおける評価細胞試験を構築した。また、耐暑性検定については、熱ストレス耐性・感受性品種と山口県育成耐暑性品種を用いて温水浸漬処理による熱ストレスに対する光合成の量的・質的反応性を調査することで、耐暑性検定が可能であることが示された。 そこで、挟式温水循環処理による熱ストレス耐性の評価系から得られる表現型値データと各種代謝物質（アミノ酸、フラボノイド類、カロテノイド類等）の定性・定量データやワイドターゲット-メタボローム解析データとの比較解析の準備を行った。


• バイオマーカーを利用した耐乾性コムギ育種法の確立および代謝物QTL解析

  妻鹿 良亮

  日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 鳥取大学, Apr. 2020 - Mar. 2024, Principal investigator

  アブシシン酸受容体遺伝子の過剰発現によって気孔を閉鎖気味に保つことによって蒸散を抑え、水の消費量を低下させることで、種子収量を維持できる「節水型耐乾性」形質をコムギが獲得できることを先行研究において見出した。しかし、遺伝子組換え作物の社会的許容の観点からも遺伝子の過剰発現では栽培系統としての確立は現在の時世的に難しい。そのため本研究では、43種類のDゲノムドナーを親に持つ多重合成コムギ派生(MSD)集団という多様な遺伝的背景を持つ自然交配系統から節水型耐乾性系統の探索を行う。節水型耐乾性コムギは非節水型と比較して、(1)植物体の葉表面温度が高い。(2) 炭素安定同位体比の値が大きい。(3) 1000粒種子重の低下が小さい。等の特徴が見られる。しかし、いずれの表現型のデータを取得するためには、ある程度の大きさ、あるいは登熟まで植物を育てる必要があるため、申請者はより簡便な選抜方法を確立することを目的に形質バイオマーカーを探索する。その候補系統を (1)~(3)の表現型を指標としてスーダンの灌漑圃場においてコントロール区と乾燥区でMSD集団の栽培を行い、その登熟した葉を採取し、炭素安定同位体比を計測し、節水性形質を評価した。また、葉表面温度や収穫後の1000粒種子重の値と照らし合わせることで、節水型耐乾性および非節水型候補系統を選抜した。これらの系統を親系統として節水型と非節水型系統間で交配を行い、組換え自殖系統(RIL)集団を作製した。本年度は集団の世代促進を行い、F7世代の集団を得た。別の節水型および非節水型系統同士の組合せで作出したRIL集団の世代促進も開始した。また、GCMSによるメタボローム解析実験系を整備した。


• Elucidation of the mechanism of morphological abnormalities and sterility of reproductive tissues caused by accumulation of abscisic acid

  Ryosuke Mega

  JSPS, KAKENHI, 若手研究, 鳥取大学, Apr. 2016 - Mar. 2019, Principal investigator

  Competitive research funding