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KATSUDA Tomohisa
Graduate School of Engineering / Department of Chemical Science and Engineering
Associate Professor

Researcher basic information

■ Research Keyword
  • Biochemical Engineering
  • 微細藻類
  • クロマトグラフィー
■ Research Areas
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering
■ Committee History
  • Apr. 2017 - Mar. 2019, The Society of Chemical Engineers, Japan, Member, Kansai Branch
  • Mar. 2012 - Feb. 2018, The Society of Chemical Engineers, Japan, Secretary, Division of Biochemical Engineering
  • Jun. 2003 - Dec. 2011, The Society for Biotechnology, Member, Kansai Branch
  • Apr. 2002 - Oct. 2011, Young Asian Biochemical Engineers’ Community, 日本事務局員
  • Oct. 2002 - Dec. 2010, 日本生物工学会関西支部, バイオテクノロジー実験講座講師
  • Apr. 2000 - Mar. 2009, 化学工学会関西支部CES21, 運営委員

Research activity information

■ Award
  • Sep. 2024 The Society of Chemical Engineers, Japan, Outstanding Paper Award of 2023, Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix
    Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji
    Official journal

  • Nov. 2023 神戸大学工学部, 令和四年度優秀教育賞（応用化学科）
    勝田知尚

  • Nov. 2018 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of influenza virus-like particles in stably transformed insect cells
    MATSUDA Takuya, TANIJIMA Toshikazu, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa, YAMAJI Hideki
    International society

  • Oct. 2018 神戸大学工学部, 平成29年度優秀教育賞（応用化学科）
    勝田 知尚

  • Sep. 2017 化学工学会, バイオ部会優秀ポスター賞, 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    Japan society

  • Nov. 2016 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of virus-like particles using recombinant insect cells
    TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki
    International society

  • Oct. 2010 Asia Pacific Confederation of Chemical Engineering, Best Poster Paper Award (The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010)), Production of Japanese encephalitis virus-like particles in insect cell expression systems
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    International society

  • Feb. 2010 化学工学会関西支部, 化学工学会バイオ部会, 第8回最先端バイオテクノロジー発表会ポスター賞, 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響, 野上達弘
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  • Dec. 2009 Young Asian Biochemical Engineers’ Community, Best Poster Presentation Award for an Original Contribution to the 15th Symposium of Young Asian Biochemical Engineers’ Community, Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli
    KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  • Sep. 2007 神戸大学工学部, 平成18年度優秀教育賞（移動現象論・分離工学分野）
    大村 直人, 今駒 博信, 鈴木 洋, 勝田 知尚

  • Sep. 2007 日本生物工学会, 生物工学奨励賞(照井賞), フォトバイオリアクターによる有用物質生産のための生物化学工学的検討
    KATSUDA Tomohisa

  • Mar. 2005 化学工学会, Masaki Naito Memorial Award, 光合成微生物による光培養生産プロセスの開発
    KATSUDA Tomohisa

■ Paper
  • Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji
    Feb. 2023, Journal of Chemical Engineering of Japan, 56(1) (1), 2179369, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Luca Giannelli, Chihiro Watanabe, Hideki Yamaji, Tomohisa Katsuda
    Computational fluid dynamics (CFD) was used to investigate cascade photobioreactors (cascade PBRs) with two different bottom configurations-flat and wavy-to establish the effect that fluid-flow regimes exert on the photosynthetic productivity of Chlorella sorokiniana. In the flat-bottom PBR, areal biomass productivities decreased from 6.8 to 4.2 g.m(-2).d(-1) when the flow rate of a culture per unit of lane width was increased from 33 to 132 L.m(-1).min(-1). We found that this decrease in the areal productivity was the result of a decrease in the volumetric photon flux densities (volumetric PFDs), which was caused by an increase in the depth of the culture in the lane. Through CFD calculation and long-exposure photography, the flow of the culture in the wavy-bottom PBR was characterized in an upper straightforward section and underneath the swirling section. Under identical conditions of flow rate and volumetric PFD (66 L.m(-1).min(-1) and 50 mu mol.m(-3).s(-1), respectively), the cell growth accelerated in the wavy-bottom PBR with areal productivity that reached 6.5 g.m(-2).d(-1)-productivity was 5.1 g.m(-2).d(-1) in the flat-bottom PBR. The swirling flow in the wave troughs held the culture for longer periods in the illuminated lane, and the resultant extended period of mixing improved the photosynthetic productivity.
    ELSEVIER, Nov. 2022, JOURNAL OF BIOTECHNOLOGY, 359, 15 - 20, English
    [Refereed][Invited]
    Scientific journal

  • Ryou Nakanuma, Kyoko Masumi-Koizumi, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
    Insect cells have recently proven to be an excellent platform for the high-level production of functional recombinant proteins. Autophagy is an important mechanism that promotes cell survival by eliminating damaged organelles and protein aggregates, and it also may influence recombinant protein production. In the present study, we compared the effects that autophagy inducers rapamycin, everolimus, and lithium chloride exert on recombinant lepidopteran insect cells that secrete an engineered antibody molecule. Compared with nontreatment, treatment with either rapamycin or everolimus prolonged cell growth to allow high cell density, improved viability in the declining phase, and then increased the yield of secreted antibodies. These positive effects appeared to be induced via autophagy since autophagosomes were clearly detected, particularly in cells treated with rapamycin or everolimus. Unlike rapamycin, another autophagy inducer, FK506, was ineffective in insect cells. The addition of an appropriate autophagy inducer may be effective in increasing the productivity of recombinant proteins in insect cells.
    SPRINGER, Jun. 2021, CYTOTECHNOLOGY, 73(3) (3), 299 - 305, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    Jan. 2021, MATEC Web of Conferences, 333, 07009 - 07009, English
    [Refereed]
    Scientific journal

  • Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    Virus-like particles (VLPs) are hollow nanoparticles composed of recombinant viral surface proteins without a virus genome. In the present study, we investigated the production of influenza VLPs using recombinant insect cells. DNA fragments encoding influenza A virus hemagglutinin (HA) and matrix protein 1 (M1) were cloned with the Drosophila BiP signal sequence in plasmid vectors containing a blasticidin and a neomycin resistance gene, respectively. After Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were co-transfected with a pair of constructed plasmid vectors, stably transformed cells were established via incubation with blasticidin and G418. Western blot analyses showed that recombinant High Five cells secreted HA and M1 proteins into the culture supernatant. Immunoprecipitation of the culture supernatant with an anti-HA antibody and transmission electron microscopy suggested that secreted HA and M1 proteins were in a particulate structure with a morphology similar to that of an influenza virus. Hemagglutination assay indicated that expressed HA molecules retained hemagglutination activity. In a shake-flask culture, recombinant cells achieved a high HA yield (approximate to 10 mu g/ml) comparable to the yields obtained using the baculovirus-insect cell system. Recombinant insect cells may serve as excellent platforms for the efficient production of influenza VLPs for use as safe and effective vaccines and diagnostic antigens.
    ELSEVIER, Nov. 2020, BIOCHEMICAL ENGINEERING JOURNAL, 163, 107757 - 107757, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yu Mizote, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    Antibody Fab fragments consist of heavy chain (Hc) and light chain (Lc) polypeptides assembled with a disulphide bond. The production of a recombinant Fab fragment requires the simultaneous expression of two genes encoding both an Hc and an Lc in the same host cell. In the present study, we investigated the production of Fab fragments in lepidopteran insect cells using a bicistronic plasmid vector carrying the Hc and Lc genes linked with a 2A self-cleaving peptide sequence from the porcine teschovirus-1. We also examined the arrangement of a GSG spacer sequence and a furin cleavage site sequence with the 2A sequence. Western blot analysis and enzyme-linked immunosorbent assay (ELISA) of culture supernatants showed that Trichoplusia ni BTI-TN-5B1-4 (High Five) cells transfected with a plasmid in which the Hc and Lc genes were joined by the 2A sequence successfully secreted Fab fragments with antigen-binding activity after self-cleavage of the 2A peptide. The GSG linker enhanced 2A cleavage efficiency, and the furin recognition site was useful for removal of 2A residues from the Hc. Transfection with a single plasmid that contained sequences for GSG, the furin cleavage site, GSG, and the 2A peptide between the Hc and Lc genes exhibited a higher productivity than co-transfection with a set of plasmids separately carrying the Hc or Lc gene. These results demonstrate that bicistronic expression with the appropriate combination of a furin recognition site, GSG linkers, and a 2A peptide may be an effective way to efficiently produce recombinant antibody molecules in insect cells. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Aug. 2020, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 130(2) (2), 205 - 211, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Production of influenza virus-like particles in insect cells
    Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda
    May 2020, BMC Proceedings, 14, 14 - 15, English
    International conference proceedings

  • LEONG Hui Yi, OOI Chien Wei, LAW Chung Lim, JULKIFLE Advina Lizah, KATSUDA Tomohisa, SHOW Pau Loke
    Recently, betacyanins have added research applications in food science processing industries, owing to their health promoting functional and attractive visual properties. Additionally, red-purple pitaya (Hylocereus polyrhizus) is a rich source of betacyanins. Development of a green and effective bioseparation technology is deemed favourably in the downstream bioprocessing industries. Hence, this study utilised a liquid biphasic electric flotation (LBEF) system for betacyanins extraction from peel and flesh of red-purple pitaya as well as comparison between LBF (liquid biphasic flotation) and LBEF system, color characterisation and antioxidant activity evaluation were conducted. In this study, the optimised LBF system was integrated with electric system. Collectively, the betacyanins extraction using LBEF system showed an optimal betacyanins extraction from the peel and flesh, with the significant highest values of betacyanins concentration in alcohol-rich top phase (C-t) (99.014 +/- 0.074% and 96.132 +/- 0.154%), separation efficiency (E) (98.383 +/- 0.215% and 96.576 +/- 0.083%) and partition coefficient (K) (100.814 +/- 7.324 and 24.883 +/- 1.052) of betacyanins. Moreover, the peel and flesh extract showed different variations of red colors and antioxidant properties. The present study introduces a new, simple and high efficient bioseparation technology (LBEF system) which is definitely worth to study further.
    ELSEVIER SCIENCE BV, Jan. 2019, Separation and Purification Technology, 209, 193 - 201, English
    [Refereed]
    Scientific journal

  • OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    Monoclonal antibodies and antibody fragments are widely used in therapeutics and diagnoses. While mammalian cells serve as the host cells for antibody production, insect cells can produce large quantities of secretory antibodies in serum-free suspension cultures. The effects of lithium on the processes of autophagy and apoptosis in mammalian cells are well chronicled. In the present study, stably transformed insect cells, which produce an engineered antibody molecule, were cultured with lithium chloride in a serum-free medium. Treatment with lithium chloride induced autophagy and apoptosis in recombinant insect cells and led to increases in the yields of secreted antibodies.
    SPRINGER, Jan. 2019, In Vitro Cellular & Developmental Biology - Animal, 55(1) (1), 1 - 6, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • LEE Xin Jiat, SHOW Pau Loke, KATSUDA Tomohisa, CHEN Wei-Hsin, CHANG Jo-Shu
    Membrane bioreactor (MBR) is regarded as the state-of-the-art technology in separation processes. Surface modification techniques play a critical role in improving the conventional membrane system which is mostly hydrophobic in nature. The hydrophobic nature of membranes is known to cause fouling, resulting in high maintenance costs and shorter lifespan of MBR. Thus, surface grafting aims to improve the hydrophilicity of bio-based membrane systems. This review describes the major surface grafting techniques currently used in membranes, including photo induced grafting, plasma treatment and plasma induced grafting, radiation induced grafting, thermal induced grafting and ozone induced grafting. The advantages and disadvantages of each method is discussed along with their parametric studies. The potential applications of MBR are very promising, but some integral membrane properties could be a major challenge that hinders its wider reach. The fouling issue could be resolved with the surface grafting techniques to achieve better performance of MBRs.
    ELSEVIER SCI LTD, Dec. 2018, Bioresource Technology, 269, 489 - 502, English
    [Refereed]
    Scientific journal

  • YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa
    Oct. 2018, New Biotechnology, 44S, S159, English
    International conference proceedings

  • Revathy Sankaran, Pau Loke Show, Yee Jiun Yap, Yang Tao, Tau Chuan Ling, Katsuda Tomohisa
    Liquid biphasic flotation (LBF) system has been recognized as an efficient, green, economically sustainable and biocompatible technique for biomolecules separation and purification. The main drawbacks of the conventional process of biomolecules separation are expensive production cost, utilization of phase components that are inefficiently recycled and global pollution due to high chemical consumption and wastage. In this paper, a novel approach of LBF system for lipase recovery utilizing recycling phase components comprising surfactant and xylitol was investigated. The scope of this study focuses on pollution prevention as well as clean and environmentally friendly process for enzyme extraction via LBF. The green process proposed in this study uses phase-forming components that have recovery and recycling abilities for minimal use of chemicals for enzyme extraction. This novel method utilized Triton X-100 and xylitol for lipase extraction from Burkholderia cepacia. A few parameters were optimized to obtain high lipase separation efficiency and yield. Based on the ideal conditions of LBF, the average lipase separation efficiency and yield are 86.46 and 87.49%, correspondingly. Phase components recycling were proposed in order to reduce the chemicals consumption in LBF system. Upscaling of the recycling study exhibited consistent result with the laboratory scale. It was found that 97.20 and 98.67% of Triton X-100 and xylitol were recovered after five times of recycling and that a total of 75.87% of lipase separation efficiency was obtained. Recovery and recycling of phase components in the extraction process are established as the principal green chemistry method, which yields high separation efficiency and is economically feasible on an industrial scale.
    Springer Verlag, Apr. 2018, Clean Technologies and Environmental Policy, 20(9) (9), 1 - 12, English
    [Refereed]
    Scientific journal

  • YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa
    Mar. 2018, BMC Proceedings, 12(3) (3), 19, English
    International conference proceedings

  • Keita Mori, Hirotsugu Hamada, Takafumi Ogawa, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
    Transient gene expression allows a rapid production of diverse recombinant proteins in early-stage preclinical and clinical developments of biologics. Insect cells have proven to be an excellent platform for the production of functional recombinant proteins. In the present study, the production of an antibody Fab fragment by transient gene expression in lepidopteran insect cells was investigated. The DNA fragments encoding heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of an Fab fragment were individually cloned into the plasmid vector plHAneo, which contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression. Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were co-transfected with the resultant plasmid vectors using linear polyethyleneimine. When the transfection efficiency was evaluated, a plasmid vector encoding an enhanced green fluorescent protein (EGFP) gene was also co-transfected. Transfection and culture conditions were optimized based on both the flow cytometry of the EGFP expression in transfected cells and the yield of the secreted Fab fragments determined by enzyme-linked immunosorbent assay (ELISA). Under optimal conditions, a yield of approximately 120 mg/L of Fab fragments was achieved in 5 days in a shake-flask culture. Transient gene expression in insect cells may offer a promising approach to the high-throughput production of recombinant proteins. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Aug. 2017, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124(2) (2), 221 - 226, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hideki Yamaji, Keita Mori, Hirotsugu Hamada, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda
    ELSEVIER SCIENCE BV, Jul. 2016, NEW BIOTECHNOLOGY, 33, S200 - S201, English
    International conference proceedings

  • Shinya Takada, Takafumi Ogawa, Kazusa Matsui, Tasuku Suzuki, Tomohisa Katsuda, Hideki Yamaji
    The generation of a recombinant baculovirus that displays antibody Fab fragments on the surface was investigated. A recombinant baculovirus was engineered so that the heavy chain (Hc; Fd fragment) of a mouse Fab fragment was expressed as a fusion to the N-terminus of baculovirus gp64, while the light chain of the Fab fragment was simultaneously expressed as a secretory protein. Following infection of Sf9 insect cells with the recombinant baculovirus, the culture supernatant was analyzed by enzyme-linked immunosorbent assay using antigen-coated microplates and either an anti-mouse IgG or an anti-gp64 antibody. A relatively strong signal was obtained in each case, showing antigen-binding activity in the culture supernatant. In western blot analysis of the culture supernatant using the anti-gp64 antibody, specific protein bands were detected at an electrophoretic mobility that coincided with the molecular weight of the Hc-gp64 fusion protein as well as that of gp64. Flow cytometry using a fluorescein isothiocyanate-conjugated antibody specific to mouse IgG successfully detected the Fab fragments on the surface of the Sf9 cells. These results suggest that immunologically functional antibody Fab fragments can be displayed on the surface of baculovirus particles, and that a fluorescence-activated cell sorter with a fluorescence-labeled antigen can isolate baculoviruses displaying specific Fab fragments. This successful baculovirus display of antibody Fab fragments may offer a novel approach for the efficient selection of specific antibodies.
    SPRINGER, Aug. 2015, CYTOTECHNOLOGY, 67(4) (4), 741 - 747, English
    [Refereed]
    Scientific journal

  • Luca Giannelli, Hiroyuki Yamada, Tomohisa Katsuda, Hideki Yamaji
    The green alga Haematococcus pluvialis, which accumulates astaxanthin at an optimal temperature of 20 degrees C, was cultivated under temperatures of 20 degrees C, 23.5 degrees C, 27 degrees C, and 30.5 degrees C, in order to assess the effects on algal metabolism during the growth phase. The culture growth rate declined with above-optimal increases in temperature, and the final maximum cell concentration at 30.5 degrees C reached only 35% of that attained at 20 degrees C. On the contrary, the biomass productivity was increased under all the high-temperature conditions, probably reflecting the metabolism switch from cell duplication to energy accumulation that is typically observed in algal cultures subjected to environmental stress. Moreover, an increase in the light-harvesting capability of the alga was observed by means of the total pigment balance and the photosynthesis-intensity (PI) curve measured under the different cultivation conditions. Cultures kept at higher temperatures were able to better harvest and utilize the impinging light due to photo-acclimation. Finally, the differences in the astaxanthin metabolism were elucidated by subjecting the cultures to nitrogen starvation at 20 degrees C and 27 degrees C. In the culture at 27 degrees C, a 1.4-fold increase in the astaxanthin productivity was observed when compared to that at 20 degrees C, and the latter required almost two-fold more energy for the astaxanthin production compared with the 27 degrees C culture. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 345 - 350, English
    [Refereed]
    Scientific journal

  • Luca Giannelli, Hideki Yamaji, Tomohisa Katsuda
    Vertical photobioreactors (PBR) with cylindrical cross section, namely air-lift reactors (ALR) and bubble column reactors (BCR), are often chosen both for bench-scale and industrial scale microalgal cultivation. It was common belief that ALR was the most favorable configuration in terms of light conversion effciency (LCE) and/or photosynthetic productivity than BCR because of the regular cyclic-flow pattern achieved inside the PBR. In the present study, we simulated the flow patterns in both ALR and BCR by means of computational fluid dynamics (CFD) and clarified the effects of such flow pattern on the LCE and productivity. Simulation results, obtained from the open-source CFD suite OpenFOAM, showed good agreement both for the flow velocity and the mixing time observed in the actual PBR using high-speed photography and conductivity pulse response, respectively. Subsequently, Lagrangian particle tracking was conducted on the simulation results to highlight the main fluid-flow patterns and to calculate the local-flashing-light frequency, which was necessary in order to estimate the overall light conversion effciency of the PBR. The BCR was characterized by a highly random fluid pattern with macroscopic, low-frequency circular loops while the ALR was characterized by numerous swirling flows localized inside the draft tube in addition to the main recirculation between the inner and outer portions of the tube. Finally, image analysis was used to correlate the numerical calculations with the light conversion effciencies attained in a Haematococcus pluvialis culture that had been illuminated with flashing light.
    SOC CHEMICAL ENG JAPAN, Jan. 2015, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 48(1) (1), 61 - 71, English
    [Refereed]
    Scientific journal

  • Hideki Yamaji, Tomohisa Katsuda, Hirotsugu Hamada, Keita Mori
    ELSEVIER SCIENCE BV, Jul. 2014, NEW BIOTECHNOLOGY, 31, S186 - S186, English
    International conference proceedings

  • Hideki Yamaji, Masataka Nakamura, Miwa Kuwahara, Yusuke Takahashi, Tomohisa Katsuda, Eiji Konishi
    The production of Japanese encephalitis (JE) virus-like particles (VLPs) in stably transformed lepidopteran insect cells was investigated. The DNA fragment encoding the JE virus (JEV) prM signal peptide, the precursor (prM) of the viral membrane protein (M), and the envelope glycoprotein (E) was cloned into the plasmid vector pIHAbla. The pIHAbla contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with a blasticidin resistance gene for use as a selectable marker. DNA encoding a form of prM with a pr/M cleavage site mutation was used to suppress the cell-fusion activity of VLPs. After transfection with the resultant plasmid, Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were incubated with blasticidin, and cells resistant to the antibiotic were obtained. Western blot analysis and enzyme-linked immunosorbent assay of a culture supernatant showed that transfected High Five cells secreted an E antigen equivalent to the authentic JEV E. Sucrose density-gradient sedimentation analysis of the culture supernatant from recombinant High Five cells indicated that secreted E antigen molecules were produced in a particulate form. VLPs recovered from the supernatant successfully induced neutralizing antibodies in mice, particularly when adsorbed to alum adjuvant. High yields (a parts per thousand 30 mu g/ml) of E antigen were achieved in shake-flask cultures. These results indicate that recombinant insect cells may offer a novel approach for efficient VLP production.
    SPRINGER, Feb. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(3) (3), 1071 - 1079, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hideki Yamaji, Maiko Segawa, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi
    The production of a secreted form of Japanese encephalitis (JE) virus-like particles (VLPs) using the baculovirus-insect cell system was investigated. A recombinant baculovirus that contained the JE virus (JEV) prM signal sequence and the genes encoding the precursor (prM) of the viral membrane protein (M) and the envelope glycoprotein (E) was constructed. Western blotting and enzyme-linked immunosorbent assay (ELISA) of the culture supernatant showed that Spodoptera frugiperda Sf9 cells infected with the recombinant baculovirus had secreted the E protein. Sucrose density-gradient sedimentation analysis of the culture supernatant suggested that secreted E antigen molecules were in a particulate form. Baculovirus-infected Sf9 cells produced more than a 10-fold higher yield of E antigen than that produced by previously reported recombinant CHO cells. Following infection with a recombinant baculovirus encoding a form of prM with a pr/M cleavage site mutation designed to suppress cell-fusion activity of E, Sf9 cells showed an E antigen yield comparable to a yield obtained with the baculovirus encoding the authentic form of prM. Baculovirus-infected Trichoplusia ni BIT-TN-5B1-4 (High Five) cells secreted less of the E antigen than Sf9 cells. Moreover, the Drosophila BiP signal sequence gave an E antigen yield comparable to the prM signal sequence, while the honeybee melittin signal sequence and the baculovirus gp64 signal sequence resulted in lower yields of the E antigen. These results provide information important to the development of VLP production processes using the baculovirus insect cell system. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(6) (6), 657 - 662, English
    [Refereed]
    Scientific journal

  • Development of Liposome Preparation, Using Gas-Liquid Phase Adsorption
    Hirohiko AIHARA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Ruri HIDEMA
    Nov. 2012, International Workshop on Process Intensification 2012, 51 - 52, English
    International conference proceedings

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji
    We describe the secretory production of a mouse anti-bovine ribonuclease A single-chain variable fragment (scFv) fused with the Fc region of human IgG1 in stably transformed lepidopteran insect cells. Use of the insect-derived Bip and melittin signal peptides resulted in higher yields of the secreted scFv-Fc fusion protein than use of the baculovirus gp64 signal peptide. After cotransfection with an expression vector that contains the Bip signal sequence upstream of the DNA encoding the scFv-Fc and a selection vector that carries a neomycin resistance gene, Trichoplusia ni BT1-TN-5B1-4 (High Five) cells were cultured in the presence of G418. Colonies of resistant cells were obtained around 2 weeks after adding G418, and clonal cells were screened by enzyme-linked immunosorbent assay (ELISA) of the culture supernatant. The yield of the scFv-Fc protein secreted from the most productive clone was around 60 mg/l in a shake-flask culture. To improve the productivity, we investigated the effect of medium supplements of sodium butyrate (NaBu), dimethyl sulfoxide (DMSO), and sericin. Supplementing culture medium with sericin increased the scFv-Fc protein yield to 82 mg/l, but productivity was not increased by either NaBu or DMSO. These results indicate that the stably transformed insect cells may allow for the efficient production of scFv-Fc and other Fc fusion proteins. (C) 2012 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE SA, Aug. 2012, BIOCHEMICAL ENGINEERING JOURNAL, 67(-) (-), 77 - 82, English
    [Refereed]
    Scientific journal

  • Tomohisa Katsuda, Hiroyuki Sonoda, Yoichi Kumada, Hideki Yamaji
    Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine-Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed.
    2012, Methods in molecular biology (Clifton, N.J.), 907, 305 - 24, English, International magazine
    Scientific journal

  • Hiroyoshi Miyahara, Ryou Nakashima, Masaki Inoue, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh
    A silica-based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica-based medium were studied during repeated use and compared with other protein A media available on the market. The silica-based medium provided high dynamic binding capacities for human polyclonal IgG in the superficial liquid velocity range from 150 to 720?cm?h1 because of a high mass transfer coefficient and high mechanical strength. The dynamic binding capacity and IgG recovery were high and varied only slightly during 50150 cycles of repeated use. The release of protein A ligand was low.
    WILEY-BLACKWELL, Jan. 2012, CHEMICAL ENGINEERING & TECHNOLOGY, 35(1) (1), 157 - 160, English
    [Refereed]
    Scientific journal

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji
    The effects of cytoplasmic and periplasmic chaperones on the secretory production of an anti-bovine ribonuclease A single-chain variable fragment (scFv) 3A21 in Escherichia coli were investigated. Co-expression of a cytoplasmic chaperone, GroEL/ES, DnaK/DnaJ/GrpE, trigger factor, or SecB with 3A21 scFv affected the proportions of antigen-binding activity in the cytoplasmic soluble fraction, the periplasmic fraction, and the extracellular medium, but there was no significant difference in the total activity compared to the control without chaperone co-expression. On the other hand, co-expression of a periplasmic chaperone, Skp or FkpA, with the exception of DsbC, greatly increased the binding activity in all the soluble fractions. Co-expression of both Skp and FkpA had no synergistic effect. Combinations of cytoplasmic and periplasmic chaperones decreased the productivity. In shake-flask cultures of cells co-expressing Skp or FkpA, considerable amounts of 3A21 scFv were detected in the extracellular medium by enzyme-linked immunosorbent assay (ELISA) and Western blot, and the extracellular production level of 3A21 scFv was calculated to be around 40mg/l. The binding activity of 3A21 scFv co-expressed with Skp was slightly higher than that with FkpA. These results indicate that the co-expression of periplasmic chaperones Skp and FkpA is extremely useful for the secretory production of scFvs in a culture medium using E. coli, but cytoplasmic chaperones and multiple-chaperone combinations may not be effective. Copyright (C) 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Apr. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(4) (4), 465 - 470, English
    [Refereed]
    Scientific journal

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji
    We describe the soluble production of a mouse anti-bovine ribonuclease A single-chain variable fragment (scFv) fused with the Fc region of human IgG1 in Escherichia coli. Two production systems, secretory production using a pelB signal peptide and cytoplasmic production in a trxB/gor double mutant strain with an oxidizing cytoplasm, were investigated for efficient production of soluble and functional scFv-Fc fusion protein. Antigen-binding activity was observed in both systems but almost all of the scFv-Fc that was expressed formed insoluble aggregates. Hence, the co-expression of molecular chaperones was examined. Co-expression of GroEL/GroES showed a 4.6-fold increase in antigen-binding activity in the cytoplasmic production system but not in the secretory system. By contrast, the other two chaperones, DnaK/DnaJ/GrpE and trigger factor, had no effect in either production system. The protein solubility was also improved markedly by the co-expression of GroEL/GroES and approximately 70% of the 3A21 scFv-Fc protein was soluble. A practical productivity of more than 10 mg/L was achieved with a simple batch shake-flask culture. These results indicate that the E. coli cytoplasmic production system with oxidizing cytoplasm and molecular chaperones might be one of the choices for the soluble production of scFv-Fcs and other Fc fusion proteins. (c) 2010 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Feb. 2011, BIOCHEMICAL ENGINEERING JOURNAL, 53(3) (3), 253 - 259, English
    [Refereed]
    Scientific journal

  • Takanori Furuta, Takafumi Ogawa, Tomohisa Katsuda, Ikuo Fujii, Hideki Yamaji
    The production of an Fab fragment of the catalytic antibody 6D9 in lepidopteran insect cells infected with a recombinant baculovirus that contained both the heavy chain (Hc) and light chain (Lc) genes of the Fab fragment was investigated. Western blotting and enzyme-linked immunosorbent assay (ELISA) of culture supernatant showed that baculovirus-infected Trichoplusia ni BTI-TN-5B1-4 (High Five) cells secreted an Fab fragment that retained antigen-binding activity. Infection of High Five cells with a recombinant baculovirus, in which the Lc and Hc genes were located downstream of the baculovirus p10 and polyhedrin promoters, respectively, produced a higher Fab fragment yield than that obtained with a baculovirus in which the Hc and Lc genes were downstream of the p10 and polyhedrin promoters, respectively. Baculovirus-infected High Five cells secreted more of the Fab fragment than Spodoptera frugiperda Sf9 cells. Moreover, use of the baculovirus gp64 signal sequence upstream of the Lc and Hc genes resulted in greater yield of the secreted Fab fragment than use of the insect-derived BiP and melittin signal sequences. Consequently, the Fab fragment was obtained in a high yield (>600 mu g/ml) in a shake-flask culture of High Five cells infected at a multiplicity of infection (MOI) of 10 plaque-forming units (pfu)/cell with the recombinant baculovirus in which the Lc and Hc genes with the gp64 signal sequence were expressed under the control of the p10 and polyhedrin promoters, respectively. These results indicate that the baculovirus-insect cell system may allow efficient production of antibody Fab fragments. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Nov. 2010, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 110(5) (5), 577 - 581, English
    [Refereed]
    Scientific journal

  • Micro-fluidic behavior on liposome production in a micro-channel
    Atsushi Fujiwara, Hiroshi Suzuki, Tomohisa Katsuda, Yoshiyuki Komoda
    Oct. 2010, The 13th Asia Pacific Confederation of Chemical Engineering Congress Proceedings, 10338
    [Refereed]

  • Production of Japanese encephalitis virus-like particles in insect cell expression systems
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    Oct. 2010, The 13th Asia Pacific Confederation of Chemical Engineering Congress Proceedings, 10640, English
    [Refereed]
    International conference proceedings

  • Naoya Morishita, Tomohisa Katsuda, Shuji Kubo, Akinobu Gotoh, Hideki Yamaji
    Adenovirus vector production by anchorage-independent 293 cells immobilized using porous biomass support particles (BSPs) was investigated in static and shake-flask cultures for efficient large-scale production of adenovirus vectors for gene therapy applications. The density of cells immobilized within BSPs was evaluated by measuring their WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) reduction activity. In shake-flask culture, 293-F cells, which were adapted to serum-free suspension culture, were not successfully retained within reticulated polyvinyl formal (PVF) resin BSPs (2 x 2 x 2 mm cubes) with matrices of relatively small pores (pore diameter 60 mu m). When the BSPs were coated with a cationic polymer polyethyleneimine, a high cell density of more than 10(7) cells cm(-3)-BSP was achieved in both static and shake-flask cultures with regular replacement of the culture medium. After infection with an adenovirus vector carrying the enhanced green fluorescent protein gene (Ad EGFP), the specific Ad EGFP productivity of the immobilized cells was comparable to the maximal productivity of non-immobilized 293-F cells by maintaining favorable conditions in the culture environment.
    SPRINGER, Aug. 2010, CYTOTECHNOLOGY, 62(4) (4), 293 - 300, English
    [Refereed]
    Scientific journal

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji
    The production of a single-chain variable fragment (scFv) antibody against bovine ribonuclease A in the cytoplasm of Escherichia coli trxB/gor double mutant was investigated. Previous reports have shown that the thioredoxin (Trx) protein fusion strategy is useful for the correct folding of scFvs and that the expression of functional scFvs is increased by co-expression of molecular chaperones. In the present study, we examined the effects of the combination of Trx fusion and molecular chaperone co-expression on the production of a functional scFv. A Trx-fused scFv was obtained in the oxidizing cytoplasm, and co-expression of GroELS and trigger factor had the greatest effect, resulting in a 2.8-fold increase in specific productivity. By contrast, the molecular chaperone DnaKJE had no effect. Moreover, co-expression of DnaKJE with GroELS negated the effects of GroELS. Trx-scFv was purified using a bovine ribonuclease A-coupled Sepharose column, and 2.7 mg/L of purified protein was obtained. Soluble Trx-scFv, expressed and purified as described above, exhibited pH-dependent binding similar to that of the parental full-length antibody. In addition, approximately 80% of the initial binding activity was retained after incubation at 37 degrees C for 2 weeks, indicating that the Trx-scFv fusion protein is quite stable. This strategy might be useful for the preparation of other recombinant scFvs. (C) 2009 Elsevier Inc. All rights reserved.
    ACADEMIC PRESS INC ELSEVIER SCIENCE, Apr. 2010, PROTEIN EXPRESSION AND PURIFICATION, 70(2) (2), 248 - 253, English
    [Refereed]
    Scientific journal

  • Mayu Fukui, Shinji Shiomi, Naoki Tanuma, Tomohisa Katsuda, Naofumi Shiomi, Hideki Yamaji
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S93 - S94, English
    [Refereed]
    International conference proceedings

  • Hideki Yamaji, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(S1) (S1), S7 - S7, English
    International conference proceedings

  • Hasan M. Fida, Yoichi Kumada, Masaaki Terashima, Tomohisa Katsuda, Shigeo Katoh
    It is common for small tandem peptide multimer genes to be indirectly inserted into expression vectors and fused with a protein tag. In this study, a multimer of the tandem angiotensin I-converting enzyme inhibitory peptide (ACE-IP) gene was directly transferred to a commercially available vector and the designed gene was expressed as a repeated peptide in Escherichia coli BL21(DE3)pLysS. The process further developed in our study was the construction of six-repeated ACE-IP synthetic genes and their direct insertion. Protein expression in inclusion bodies was confirmed by SDS-PAGE and Western blot. Acid hydrolysis of inclusion bodies produced single-unit peptides through cleavage of the aspartyl-prolyl bonds. This cleaved recombinant peptide (rACE-IP) was purified using immuno-affinity chromatography followed by reversed phase-HPLC. 105-115 mg of the lyophilized recombinant peptide was obtained from 1 L E. coli culture. In vitro biological activity of rACE-IP was indistinguishable from that of the natural peptide produced by hydrolysis in artificial gastric juice or by acidic hydrolysis. The rACE-IP prepared by recombinant DNA technology and solid-phase synthesis methods showed a similar IC 50. This strategy could be used for the expression of important peptides, which have N-terminal proline (P) and C-terminal aspartic acid residues (D) for commercial applications, e.g. functional foods and drinks. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Sep. 2009, Biotechnology Journal, 4(9) (9), 1345 - 1356, English
    [Refereed]
    Scientific journal

  • Juan Huang, Na Jin, Tomohisa Katsuda, Hideki Fukuda, Hideki Yamaji
    Escherichia coli cells were efficiently immobilized in reticulated polyvinyl formal resin biomass support particles (BSPs) that had been simply autoclaved with a solution of a cationic polymer such as polyethyleneimine. When the immobilized E. coli cells containing aspartase were used as whole cell biocatalyst for L-aspartic acid production, they showed specific aspartase activity comparable to that of non-immobilized cells. (C) 2009 Elsevier B. V. All rights reserved.
    ELSEVIER SCIENCE SA, Sep. 2009, BIOCHEMICAL ENGINEERING JOURNAL, 46(1) (1), 65 - 68, English
    [Refereed]
    Scientific journal

  • Kazutaka Ikeda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh
    A single-chain variable fragment (scFv) antibody against bisphenol A was refolded using an antigen (bisphenol A)-coupled column. The refolding efficiency was compared with that used in dialysis. The refolding efficiency of the antigen-coupled column was about 50-60%, which was much higher than with dialysis, due to a decrease in the concentration of the refolding molecules and to the suppression of the aggregate formation. (C) 2009 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE SA, May 2009, BIOCHEMICAL ENGINEERING JOURNAL, 44(2-3) (2-3), 289 - 291, English
    [Refereed]
    Scientific journal

  • Kentaro Yamada, Naoya Morishita, Tomohisa Katsuda, Shuji Kubo, Akinobu Gotoh, Hideki Yamaji
    The use of low-multiplicity infection of 293 cells in static culture with regular medium replacement was investigated for efficient large-scale production of adenovirus vectors for gene therapy applications. An adenovirus vector carrying the enhanced green fluorescent protein gene (Ad EGFP) was used to infect 293-F cells at a low multiplicity of infection (MOI) of 0.00001-0.1 transductional unit (TU) cell(-1). The cells, which have the ability to grow in suspension, were incubated in T-flasks and the serum-free culture medium was replaced with fresh medium via centrifugation every 2 days. Because only a small proportion of cells were initially infected at low MOIs (< 1 TU cell(-1)), uninfected cells continued to grow until they were infected by progeny adenoviruses released from previously infected cells. When 293-F cells at a relatively low density of 1 x 10(5) cells cm(-3) were infected with Ad EGFP at a low MOI of 0.001 TU cell(-1), the vector yield was 2.7-fold higher than the maximum yield obtained with high-multiplicity infection (MOI = 10 TU cell(-1)) in batch culture. These results indicate that efficient adenovirus vector production using low MOIs is achieved by minimization of either nutrient depletion and/or accumulation of inhibitory metabolites in the culture medium.
    SPRINGER, Apr. 2009, CYTOTECHNOLOGY, 59(3) (3), 153 - 160, English
    [Refereed]
    Scientific journal

  • Yuka Kobayashi, Tadashi Yamauchi, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh
    Angiotensin-1 converting enzyme (ACE) plays important roles in the regulation of blood pressure, and ACE inhibitory peptides in food materials have attracted attention for their antihypertensive function. In this study, the function of amino acids in ACE inhibitory tripeptides was clarified.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Sep. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(3) (3), 310 - 312, English
    [Refereed]
    Scientific journal

  • RANJBAR Reza, INOUE Ryota, KATSUDA Tomohisa, YAMAJI Hideki, KATOH Shigeo
    In photobioreactors, photosynthetic microorganisms are exposed to certain light/dark cycles caused by light intensity distribution and mixing inside the photobioreactor. In this study, Haematococcus pluvialis was cultivated in an airlift and a bubble column photobioreactor, and the cell growth and astaxanthin production were compared to clarify the effects of liquid circulation.
    The Society for Biotechnology, Japan, Aug. 2008, Journal of bioscience and bioengineering, 106(2) (2), 204 - 207, English

  • Hiroshi Suzuki, Jun-ya Hamamura, Tomohisa Katsuda, Yoshiyuki Komoda, Sigeo Katoh, Hiromoto Usui
    The characteristics of liposome formation in a micro-tube have been studied to develop a novel production method that forms the liposomes with a desired size homogeneously. In this method, the inner wall of the micro-tube was used to spread the liposomal lipid on. Then, instead of conventional rigorous agitation, an aqueous solution was fed through the tube, which peeled off the thin lipid layer formed liposomes. This resulted in a sharp peak in the size-distribution profile, and the size of liposomes was found to be dependent on the flow velocity anti on the micro-tube size. Moreover, the Yield of the liposomes of the desired size in our method was much higher than that in the conventional method, because our method produced liposomes uniform in size. From these findings, we concluded that the friction drag in the micro-tube is applicable for the efficient production of liposomes and a microfluidics device consisting of micro-tubes is a promising tool in the production of liposomal formulations.
    SOC CHEMICAL ENG JAPAN, Aug. 2008, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 41(8) (8), 739 - 743, English
    [Refereed]
    Scientific journal

  • Reza Ranjbar, Ryota Inoue, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh
    In photobioreactors, photosynthetic microorganisms are exposed to certain light/dark cycles caused by light intensity distribution and mixing inside the photobioreactor. In this study Haematococcus pluvialis was cultivated in an airlift and a bubble column photobioreactor, and the cell growth and astaxanthin production were compared to clarify the effects of liquid circulation.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Aug. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(2) (2), 204 - 207, English
    [Refereed]
    Scientific journal

  • 微生物の光合成を利用した有用物質生産－エネルギーと食品への活用－
    勝田知尚
    Jul. 2008, Microoptics News, 26, 29 - 34, Japanese
    [Invited]
    Symposium

  • Reza Ranjbar, Ryota Inoue, Hironori Shiraishi, Tomohisa Katsuda, Shigeo Katoh
    Haematococcus pluvialis was cultivated under photoautotrophic conditions in a bubble column with fed-batch addition of nutrients, especially nitrate, and a cell number above 5 x 106 cells mL(-1) was attained after 300 h. The reduction of nutrient concentrations accompanied by dilution of the fermentation broth and an increase in the light intensity enhanced accumulation of astaxanthin. The final astaxanthin concentration of 390 mg L-1 was several times higher than ever reported. This combination of fed-batch addition of nutrients and dilution of broth for nutrient deficiency is a promising method for attainment of high cell and astaxanthin concentrations in a bubble column photobioreactor. (c) 2007 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE SA, May 2008, BIOCHEMICAL ENGINEERING JOURNAL, 39(3) (3), 575 - 580, English
    [Refereed]
    Scientific journal

  • フォトバイオリアクターによる有用物質生産のための生物化学工学的検討
    KATSUDA Tomohisa
    日本生物工学会, Mar. 2008, 生物工学会誌, 86(3) (3), 117 - 122, Japanese
    [Invited]
    Research society

  • Tomohisa Katsuda, Hironori Shiraishi, Naoki Ishizu, Reza Ranjbar, Shigeo Katoh
    Flashing light from blue light emitting diodes is an effective method for the reduction of energy consumption in the bioproduction of astaxanthin by Haematococcus pluvialis. We investigated the effects of light intensity and frequency on the final astaxanthin concentration in bioproduction by H. pluvialis grown mixotrophically. The final astaxanthin concentration under illumination with flashing light, with frequencies ranging from 25 to 200 Hz, was dependent on the light intensity and on the duty cycle and was equivalent, or higher, in comparison with that under illumination with continuous light at the same incident intensity. The light intensity determined the maximum attainable concentration of astaxanthin under continuous illumination. Under illumination with flashing light, the ratio of the final astaxanthin concentration to the maximum concentration at a specific light intensity was correlated to the duty cycle in the frequency range from 25 to 200 Hz. The effect of lower frequencies on enhanced astaxanthin production under flashing light was also studied; at levels as low as I Hz, higher final astaxanthin concentrations were observed under flashing light compared to concentrations attained under continuous light.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Mar. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105(3) (3), 216 - 220, English
    [Refereed]
    Scientific journal

  • Liposome formation characteristics in a micro-tube
    J. Hamamura, H. Suzuki, T. Katsuda, Y. Komoda, S. Katoh, H. Usui
    Nov. 2007, The 8th Asia-Pacific Biochemical Engineering Conference 2007 (Taipei, Taiwan, 2007), C0177
    [Refereed]

  • Shinya Yamawaki, Takehiro Matsumoto, Yuka Ohnishi, Yoichi Kumada, Naofumi Shiomi, Tomohisa Katsuda, Eun Kyu Lee, Shigeo Katoh
    This study examined the effects of two methods of methanol feeding, DO-stat and methanol concentration control, in fed-batch and continuous cultures of Pichia pastoris on cell growth and single-chain variable fragment antibody (scFv) expression. By maintaining the methanol concentration at 3.9 g l-1 in fed-batch culture, a scFv concentration of 198 mg l-1 was obtained. In continuous culture using both methanol feeding methods, the scFv concentration in the fermentation broth increased with a decreasing dilution rate. A maximum scFv concentration of 810 mg l-1 at a dilution rate of 0.0094 h-1 was obtained by maintaining the methanol concentration at 3.9 g l-1. Although the specific methanol consumption rate was the same for both methods, the specific productivity of scFv was higher in methanol concentration control from 0.0094 to 0.049 h-1 than it was in DO-stat control. Therefore, continuous culture with methanol feeding by the concentration control method shows promise for the industrial scale production of recombinant proteins by Pichia pastoris. © 2007 The Society for Biotechnology, Japan.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Nov. 2007, Journal of Bioscience and Bioengineering, 104(5) (5), 403 - 407, English
    [Refereed]
    Scientific journal

  • Shigeo Katoh, Masami Imada, Naoki Takeda, Tomohisa Katsuda, Hiroyoshi Miyahara, Masaki Inoue, Shuji Nakamura
    Considering the large molecular size of IgG antibodies widely used for therapeutic applications, the pore size, pore volume and coupling density of silica-based media were optimized for the effective large-scale purification, using an antibody-protein A affinity system. Silica media, with average pore sizes from 70 nm to 140 nm and surface areas of 26-67 m(2)/g, were prepared and coupled with protein A. The static adsorption capacity and dynamic binding capacity of bovine and human IgG were measured at superficial liquid velocities ranging from 94 to 720 cm/h. The volumetric coefficient of mass transfer of the alkali-treated silica-based protein A media, with a pore size of 110nm, was four times higher than the values for cross-linked agarose media and thus had high dynamic binding capacities at high superficial liquid velocities. (c) 2007 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Aug. 2007, JOURNAL OF CHROMATOGRAPHY A, 1161(1-2) (1-2), 36 - 40, English
    [Refereed]
    Scientific journal

  • Md. Fida Hasan, Yuka Kobayashi, Yoichi Kumada, Tomohisa Katsuda, Masaaki Terashima, Shigeo Katoh
    Inhibition characteristics of tri-peptides IKW and IKY, which were obtained as angiotensin-I converting enzyme inhibitors from bonito protein, and their stability in digestive canal were studied. These IKW and IKY showed high inhibitory activities with IC50 of 0.4 and 1.0 mu M, respectively, and acted as competitive inhibitors. The tri-peptides, especially IKY, were very stable under simulated gastrointestinal digestion conditions.
    SOC CHEMICAL ENG JAPAN, Jan. 2007, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 40(1) (1), 59 - 62, English
    [Refereed]
    Scientific journal

  • Hiroyuki Yamamoto, Keishi Hada, Hideki Yamaji, Tomohisa Katsuda, Hiromu Ohno, Hideki Fukuda
    The analysis of data from analytical equipment will be an important factor in the execution of metabolomics. Self-modeling curve resolution (SMCR) is one of the theoretical techniques of chemometrics and has recently been applied to the data of hyphenated chromatography techniques. Alternating least squares (ALS) is a classical SMCR method. In ALS, however, different solutions are produced depending on randomly chosen initial values. Simulation in the present study showed that the use of a normalized constraint in calculating ALS was effective in avoiding this problem. We also improved the ALS algorithm by adding a regularized term (regularized ALS: RALS). Independent component analysis (ICA) is a comparatively new method and has been discussed very actively by information science researchers, but has still been applied only in very few cases to curve resolution problems in chemometrics studies. We applied RALS with a normalized constraint and ICA to the HPLC-DAD data of Haematococcus pluvialis metabolites and obtained a high accuracy of peak detection, suggesting that these curve resolution methods are useful for identification of metabolites in metabolomics. © 2006 Elsevier B.V. All rights reserved.
    ELSEVIER, Dec. 2006, Biochemical Engineering Journal, 32(3) (3), 149 - 156, English
    [Refereed]
    Scientific journal

  • Tomohisa Katsuda, Kazumichi Shimahara, Hironori Shiraishi, Keisuke Yamagami, Reza Ranjbar, Shigeo Katoh
    To conserve energy in the production of astaxanthin by the green alga Haematococcus pluvialis, we utilized intermittent flashing light from blue light emitting diodes (LEDs) and investigated the effects of the incident light intensity (2-12 mu mol m(-2) s(-1)), duty cycle (17-67%) and frequency (25-200 Hz) of flashing on the cell growth and astaxanthin production. In the above ranges, the final astaxanthin concentration under illumination by flashing light was significantly higher than that obtained under illumination with continuous light at the same incident intensity. For example, flashing light at an incident intensity of 8 mu mol m(-2) s(-1) gave the same final astaxanthin concentration that was obtained under continuous light illumination at 12 mu mol m(-2) s(-1), thus reducing energy consumption by 1/3. We therefore conclude that flashing light from blue LEDs is a promising illumination method for indoor algal cultivation using photobioreactors.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Nov. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(5) (5), 442 - 446, English
    [Refereed]
    Scientific journal

  • Tomohisa Katsuda, Mitsumasa Kamura, Yasushi Nishiwada, Shigeo Katoh
    A single nucleotide polymorphism (SNP) was detected by the use of affinity chromatography with a single-stranded DNA ligand and temperature gradient elution. Because of the difference in thermostability between the ligand and sample single-stranded DNAs with and without SNPs, they were eluted as a peak with a shoulder by the temperature gradient elution. The sample DNAs with SNPs were detected as low as 0.1 pmol, and the elution behavior of DNAs with and without SNPs in this chromatography was predicted by a numerical calculation method based on a thermodynamic mass transfer model.
    SOC CHEMICAL ENG JAPAN, Oct. 2006, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 39(10) (10), 1104 - 1107, English
    [Refereed]
    Scientific journal

  • Tomohisa Katsuda, Hiroshi Oshima, Masayuki Azuma, Jyoji Kato
    A water-soluble color indicator was developed for the effective screening of hydrogen-producing microorganisms. This indicator consists of a coloring agent and a water-soluble derivative of Wilkinson's catalyst. Wilkinson's catalyst, Tris(triphenylphosphine) rhodium chloride, had been developed as a catalyst for the hydrogenation of olefins. We used a sulfonate of the catalyst for the hydrogenation of coloring agent in an aqueous medium. Several coloring agents, such as methyl orange, methyl red sodium, neutral red and Evan's blue, dissolved in water together with the sulfonated catalyst showed a change in color when hydrogen gas was fed into the solution by sparging at room temperature. We confirmed that methyl orange was decolorized by biologically produced hydrogen, when the photosynthetic bacterial strain Rhodobacter capsulatus ST-410 was grown in a medium containing 0.6 mM catalyst and 0.075 mM methyl orange in test tubes of 5 ml working volume.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 220 - 226, English
    [Refereed]
    Scientific journal

  • Naofumi Shiomi, Yutaka Yamaguchi, Hiroaki Nakai, Tomoko Fujita, Tomohisa Katsuda, Shigeo Katoh
    The rates of degradation of cyanuric acid, a key intermediate in a metabolic pathway of s-triazine herbicides, were measured for Pseudomonas sp. NRRL B-12227. The rate of degradation was affected by the rate of cyanuric acid transport through cell membranes and the activity of cyanuric acid amidohydrolase inside the cells. At low concentrations of cyanuric acid, the acclimation of cells to cyanuric acid and/or added nutrients effectively enhanced the degradation rate. The strain was also applied to bioremediation using a Bioremediation with Self-Immobilization System (BSIS), in which Pseudomonas sp. NRRL B-12227 cells were co-immobilized with Bacillus subtilis, the latter of which secretes a viscous polymer, in a shallow layer of soil packed in a column. More than 70% of the Pseudomonas sp. NRRL B-12227 cells were co-immobilized with the B. subtilis in a 7.5 cm layer of the packed soil by self-aggregation. More than 60% of the 1 mM cyanuric acid supplied to the packed soil was degraded in this layer during a 72 h period.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 206 - 209, English
    [Refereed]
    Scientific journal

  • Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Yasushi Nishiwada, Shigeo Katoh
    We developed an affinity chromatographic method for simple single nucleotide polymorphism (SNP) detection by use of a single-stranded DNA-coupled column and temperature gradient elution, utilizing the difference in thermal stability between hybridized double-stranded DNAs with and without mismatched base-pairs in the course of temperature gradient elution. We studied experimentally and theoretically the elution behavior of DNAs with and without SNPs in this chromatography and proposed a numerical calculation method based on a thermodynamic dissociation model. The effects of the column volume, flow rate of eluent and heating rate of the column on elution profiles were clarified. For designing DNA ligands, mismatched base-pair positions favorable for detection of SNPs were also explored by use of hybridized DNAs coding a part of the human TP53 gene. (c) 2006 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Aug. 2006, JOURNAL OF CHROMATOGRAPHY A, 1123(2) (2), 182 - 188, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Satoshi Yoshimura, Reza Ranjbar, Ryota Inoue, Tomohisa Katsuda, Shigeo Katoh
    A new cultivation method, in which physiological responses of Haematococcus pluvialis to different intensities and wavelengths of illuminating light are utilized, is proposed. In this method, light transmitted through a cultivation vessel illuminated from the opposite side was utilized for the early-phase cultivation of H. pluvialis in another inoculated vessel that was located behind the cultivation vessel, to save time required for the growth of cells. After harvesting cells from the front vessel, the vessel that was originally behind was shifted to the position of the front vessel. The abrupt increase in light intensity caused by shift of the position induced the effective accumulation of astaxanthin. These procedures for inoculation, shift of vessels and harvest were repeated using two vessels arranged in series along the light path. After four repeated cycles of cultivation (840 h from the start of cultivation), using 2.5 cm thick vessels, astaxanthin production per unit volume and the astaxanthin content were higher than obtained in a batch cultivation with two 2.5 cm vessels.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Aug. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(2) (2), 97 - 101, English
    [Refereed]
    Scientific journal

  • F. Hasan, Y. Kumada, N. Hashimoto, T. Katsuda, M. Terashima, S. Katoh
    Kinetics of fragmentation of angiotensin-I converting enzyme inhibitory peptides obtained by digestion in gastric juice were studied under intestinal digestion conditions and their inhibitory activities were determined. A fragment IKYGD produced by digestion, as well as IKWGD synthesized, showed similar inhibitory activity to the original peptides. These peptides somehow were resistant to tryptic and/or chymotryptic digestion, and IK + aromatic amino acid might be important functional parts in some kinds of ACE inhibitory peptides.
    INST CHEMICAL ENGINEERS, Jun. 2006, FOOD AND BIOPRODUCTS PROCESSING, 84(C2) (C2), 135 - 138, English
    [Refereed]
    Scientific journal

  • R Yegani, S Yoshimura, K Moriya, T Katsuda, S Katoh
    Using a semicontinuous culture method, in which operational parameters such as cell concentration and light intensity distribution were maintained almost constant, instability of the specific growth rate of Rhodobacter capsulatus B-100, a purple bacterium, was observed to be similar to that of R. capsulatus ST-410 when cultivated under high ratios of light intensity on the illuminated side to that of the transmitted light. Such instability was not observed in the cultivation of Chlorella vulgaris, a eukaryotic green alga, even at higher cell concentrations. Under the same conditions, the increase in only the ferrous concentration from 43 mu M, the concentration in the original RCV medium, to 172 mu M sustained a stable growth, whereas Fe2+ was slightly consumed during the cultivation. Supplemental illumination with a fluorescent lamp on the transmitted side of a flat plate photobioreactor sustained a moderate level of stable growth, while a halogen lamp slightly affected the growth stability. Our results showed that an increase in Fe2+ concentration or supplemental illumination improves the growth stability of R. capsulatus.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100(6) (6), 672 - 677, English
    [Refereed]
    Scientific journal

  • A Lababpour, K Shimahara, K Hada, Y Kyoui, T Katsuda, S Katoh
    To increase the cell concentration and the accumulation of astaxanthin, the effects of the fed-batch addition of 10-fold-concentrated medium to supply nutrients, as well as illumination with blue light emitting diodes (LEDs), on cell growth and accumulation of astaxanthin were studied for the cultivation of Haematococcus pluvialis. Using the fed-batch addition method, the cell concentration increased above 1 mg-dry cell/cm(3), and under illumination with blue LEDs, the astaxanthin concentration reached approximately 70 mu g/cm(3). This method was much simpler to operate than the medium replacement method in operation and enabled us to attain a higher total yield of astaxanthin.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Sep. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100(3) (3), 339 - 342, English
    [Refereed]
    Scientific journal

  • Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis
    T. Katsuda, A. Lababpour, K. Shimahara, S. Katoh
    May 2005, The 7th Asia-Pacific Biochemical Engineering Conference 2005 (Jeju, Korea, 2005), P2-088
    [Refereed]

  • Semi-Continuous Cultivation of Photosynthetic Cells in Flat Plate Photobioreactor
    YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo
    Apr. 2005, Iranian Journal of Chemical Engineering, 2(2) (2), 15 - 21, English
    [Refereed]
    Scientific journal

  • Neutralization of acids by microorganisms co-immobilized with Bacillus subtilis in a shallow layer of model soil
    T Yasuda, N Shiomi, S Iwasaki, Y Yamaguchi, T Katsuda, S Katoh
    Fungus and bacterium, which could grow under acidic conditions, were isolated, and their characteristics of growth and neutralization of acids were studied. They could grow in acidic media (pH 4.0) containing several kinds of acids at the same rate in the medium of pH 7.2 and neutralize acids. For bioremediation of pollutants in soil, we proposed a self-immobilization method of microorganisms forming aggregates in a shallow layer of soil. These isolated strains, which showed weak aggregation and were different in size, were successfully co-immobilized with Bacillus subtilis secreting viscous polymer in a shallow layer of model soil packed in a column and could neutralize acidic solutions flowing through packed soil.
    SOC CHEMICAL ENG JAPAN, Dec. 2004, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 37(12) (12), 1445 - 1451, English
    [Refereed]
    Scientific journal

  • Effects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis
    A Lababpour, K Hada, K Shimahara, T Katsuda, S Katoh
    In order to increase the cell concentration and the accumulation of astaxanthin, the effects of nutrient concentration, pH, illumination and methods of supplying nutrients were studied for the cultivation of Haematococcuspluvialis. The replacement of media to avoid the deficiency of nutrients increased the cell concentration above 1 mg-dry cell cm(-1) without induction of astaxanthin accumulation. Illumination with blue light emitting diode lamps and nutrient starvation induced accumulation of astaxanthin, and the interactive effects of these two increased the astaxanthin concentration to 76 mug cm(-3).
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98(6) (6), 452 - 456, English
    [Refereed]
    Scientific journal

  • Astaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps
    A. Lababpour, T. Katsuda, S. Katoh
    Nov. 2004, 2004 AIChE Annual Meeting Conference Proceedings, 483b
    [Refereed]

  • T Katsuda, A Lababpour, K Shimahara, S Katoh
    The photosynthetic microalga Haematococcus pluvialis, a potential source of astaxanthin, was cultivated under illumination with LEDs emitting red (lambda(max) = 625 nm), green(lambda(max) = 525 nm), blue (lambda(max) = 470 nm), blue-purple (lambda(max) = 410 nm) and purple (lambda(max) = 380 nm) light and a fluorescent lamp, and the effects of wavelength on cell growth and astaxanthin accumulation were studied. LEDs emitting light of short wavelengths (380-470 nm) were found to induce astaxanthin accumulation of up to 5-6% per dry cell, although the induction caused the suppression of cell growth. From these results, we proposed a new strategy of cultivating H. pluvialis under illumination with red LEDs without inducing a high level of astaxanthin accumulation, and then switching to illumination with blue LEDs at a high light intensity to induce a high level of astaxanthin accumulation. (C) 2004 Elsevier Inc. All rights reserved.
    ELSEVIER SCIENCE INC, Jul. 2004, ENZYME AND MICROBIAL TECHNOLOGY, 35(1) (1), 81 - 86, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • T Katsuda, R Yegani, N Fujii, K Igarashi, S Yoshimura, S Katoh
    For cultivation of photosynthetic cells under defined light intensity distributions, the repeated batch culture, in which a part of culture broth containing grown cells was repeatedly replaced at predetermined time intervals with a fresh medium to keep the cell concentration constant at an initial value, was employed. By use of this method the effects of the light intensity distribution on the growth characteristics of Rhodobacter capsulatus were studied. Unexpected decreases in the specific growth rate were observed in culture of R. capsulatus at high cell concentrations and a long light path length. Big differences in the light intensities of lightly and darkly illuminated portions in photobioreactors, which reflects the light intensity distribution, seemed to cause this phenomenon, which must be taken into consideration for stable growth of photosynthetic cells.
    AMER CHEMICAL SOC, May 2004, BIOTECHNOLOGY PROGRESS, 20(3) (3), 998 - 1000, English
    [Refereed]
    Scientific journal

  • YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo
    From engineering point of view, the effect of light intensity distribution on the stability of growth rate can play an important role in designing of effective photobioreactors and sustaining of stable growth rates. In these series of experiments, in order to keep the operational parameters at almost constant level, a semi-continuous culture method was developed. In this cultivation method, a part of culture broth containing grown cells was repeatedly replaced with the fresh medium at a predetermined time interval to keep the cell concentration and the volume of the broth constant at initial values. Thus it was possible to cultivate photosynthetic cells under defined light intensity distributions.
    Under one side and both side illumination and also reflection by mirror photosynthetic cells were cultivated in flat plate photobioreactors with various light path lengths. The obtained results showed that stability of the growth rate strongly depended on the distribution of light intensity and ratio of light intensity at light zone to that at dark zone inside photobioreactors. These parameters should be taken into consideration for stable cultivation of photosynthetic cells.
    The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 3P-01-085, 807 - 807, English
    [Refereed]
    International conference proceedings

  • Abdolmajid Lababpour, HADA Keishi, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo
    Haematococcus pluvialisis is one of the potential candidates for production of astaxanthin. Growth characteristics of H. pluvialis in batch run and medium replacement runs were measured. A two-stage process was employed for biomass and astaxanthin synthesis by this alga. During the first stage, medium was replaced several times every day to achieve high cell concentration. When the replacement was stopped, the light illumination was switched from red LEDs at 8μmol photons m-2 s-1 to blue LEDs at 12µmol photons m-2 s-1 to stimulate the accumulation of astaxanthin without decreasing in the cell numbers by death of the cells. H. pluvialis exhibited relatively high cell concentration in the first step and reached to about 1.5 mg-dry cell/cm3 and accumulated up to 65 µg/cm3 astaxanthin in the second stage, which are higher than those reported in literature.
    The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 3L-07, 430 - 430, English
    [Refereed]
    International conference proceedings

  • KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, Zakir Hossain, KATOH Shigeo
    In the present work, we investigated the effects of GC content, mismatching position, and length on the thermostability of double-strand oligo-DNAs in order to develop an affinity chromatography using oligonucleotide-immobilized capillary for SNP detection. We found that a single mismatching base pair appeared on 25 mer fragment of human tp53 tumor suppressor protein gene could reduce the melting temperature by 6.6°C. It seemed to be enough for eluting the mutated fragment separately from the intact one during a course of temperature ascending process.
    The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 1P-01-009, 479 - 479, English
    [Refereed]
    International conference proceedings

  • Characteristics of neutralization of acids by newly isolated fungal cells
    N Shiomi, T Yasuda, Y Inoue, N Kusumoto, S Iwasaki, T Katsuda, S Katoh
    Soil microorganisms play an important role in maintaining soil pH at levels suitable for other soil organisms. To clarify the biological neutralization mechanism in soil, we isolated soil microorganisms showing a high ability to neutralize acids and studied their characteristics. From our taxonomic study, three isolated strains were identified as filamentous fungi, namely Mucor sp., Aspergillus fumigatus, and Aureobasidium pullulans. These strains could secrete basic materials, such as ammonia, for neutralization, grow in the medium at pH 4.0 and increase the pH of the medium to approximately 8.0. These microbial cells could neutralize not only nitric acid but also sulfuric and hydrochloric acids. The strains could also grow by utilizing nitric acid as a sole nitrogen source. In the soil containing these organisms, the pH was maintained in the neutral range by the buffering action of basic materials that they secrete. These results suggest that these fungal cells are useful for protecting the soil from acidification by acid rain.
    SOC BIOSCIENCE BIOENGINEERING JAPAN, Jan. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(1) (1), 54 - 58, English
    [Refereed]
    Scientific journal

  • Cultivation of Microalga in Photobioreactors Illuminated with LEDs
    S. Katoh, A. Lababpour, T. Katsuda
    Jun. 2003, Chemical Engineering Transactions, 3, 1671 - 1675
    [Refereed]

  • Mahmoud Farshbaf, Yoshihiro Katoh, Taro Udaka, Tomohisa Katsuda, Hideo Noda, Shigeo Katoh
    Taylor-eddy and paddle type apparatus were applied for refolding of lysozyme in a continuous system. The number of theoretical mixing stages was measured for both the apparatus and the effect of flow rate and mixing rate on the axial dispersion of solutions flowing inside of a ceramic membrane tube were investigated. Refolding at different concentrations of lysozyme was performed by addition of denatured lysozyme into refolding buffers flowing through the inside of the tube. For studying the effect of addition, different addition methods of denatured lysozyme into refolding buffer were devised by using ceramic membranes, from the total surface of the membrane tube and partially upside ceramic membrane. Comparing the efficiencies of continuous refolding with those of batch method, higher refolding efficiencies were obtained in the continuous system at the same concentrations of lysozyme and urea. Therefore, by combining the merits of the fed-batch method and continuous system, the refolding efficiency can be improved as well as throughput of refolding process.
    Oct. 2002, Journal of Chemical Engineering of Japan, 35(10) (10), 963 - 968, English
    [Refereed]
    Scientific journal

  • Light attenuation in suspension of the purple bacterium Rhodobacter capsulatus and the green alga Chlamydomonas reinhardtii
    T Katsuda, N Fujii, N Takata, H Ooshima, S Katoh
    A simple and rapid calculation method, which is based on the Beer-Lambert law and Rose and Lloyd's hypothesis, was proposed for the determination of light attenuation profiles in suspensions of photosynthetic microorganisms. In this method, extinction coefficients measured spectrophotometrically at 2 single concentration of suspended cells over the whole range of wavelengths emitted from different light sources were used for reflecting the physical and biological characteristics of cells, which may change during a course of cultivation. The validity of this approach was confirmed by use of suspensions of two kinds of photosynthetic cells, the purple bacterium Rhodobacter capsulatus and the green alga Chlamydomonas reinhardtii, illuminated by three light sources with different irradiation spectra. This approach can estimate well the profiles of the light attenuation in these systems, irrespective of the spectra of the light sources, until the transmitted light intensity falls to one tenth of the incident intensity.
    SOC CHEMICAL ENG JAPAN, May 2002, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 35(5) (5), 428 - 435, English
    [Refereed]
    Scientific journal

  • Control of aggregate formation in refolding of carbonic anhydrase at high urea concentrations and effects of urea removal
    M Farshbaf, Y Katoh, T Morimoto, T Udaka, T Katsuda, S Katoh
    Refolding of CAB was studied at high concentrations of protein and urea. Aggregate formation was decreased by using higher concentrations of urea in refolding buffer. Refolding yields of 60% were obtained at high concentrations of CAB by using urea concentrations of 3.0-4.0 M in renaturation mixture, Comparison of the refolding yield and the amount of soluble CAB in renaturation mixture indicated that the apparent specific activity of refolded CAB was lower than that of native one at higher concentrations of urea. Difference in the steric structures of native and refolded CAB was observed in CD spectra of these samples. However, removal of urea from loosely folded CAB solution by dialysis recovered the native structure of CAB, as well as its native specific activity. Hence the refolding efficiency after dialysis was improved to about 70-80% at CAB concentrations of 3-5 kg/m(3). Effect of incubation time of renaturation mixture before dialysis was studied, and it was shown that incubation time less than 3 hours increased aggregate formation and decreased the refolding yield. Therefore, formation of more stable intermediates in the refolding pathway decreases aggregate formation during urea removal. These results lead to a consecutive dilution-dialysis method for refolding at high concentrations of proteins.
    SOC CHEMICAL ENG JAPAN, Jun. 2001, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 34(6) (6), 743 - 747, English
    [Refereed]
    Scientific journal

  • 光合成微生物の懸濁液中における光強度減衰
    勝田知尚, 加藤滋雄
    Apr. 2001, 神戸大学ベンチャー・ビジネス・ラボラトリー年報, 6, 185 - 190, Japanese
    Research institution

  • Tomohisa Katsuda, Takeshi Arimoto, Koichi Igarashi, Masayuki Azuma, Jyoji Kato, Susumu Takakuwa, Hiroshi Ooshima
    The light distribution in the externally illuminated cylindrical photo- bioreactor for production of hydrogen by a photosynthetic bacterium Rhodobacter capsulatus ST-410 was estimated. The estimation was performed on the basis of the Matsuura and Smith's diffuse model [1]. In the diffuse model, the incident light rays are assumed to proceed in every direction and the local intensity is calculated as the sum of the intensities of light. Since Lambert-Beer's law, extensively used in photometry, was not useful for explaining the decrease in the intensity of light by the biomass, an empirical expression was used. The measurement of the intensities from every direction was conducted in an externally illuminated cylindrical photo- bioreactor having an inner diameter of 60 mm and a working volume of 550 ml. The obtained results confirmed our estimation. The light distribution was applied to estimate the hydrogen production by R. capsulatus ST-410 using the same photo-bioreactor. The overall hydrogen-production rate was successfully estimated. (C) 2000 Elsevier Science S.A.
    Jun. 2000, Biochemical Engineering Journal, 5(2) (2), 157 - 164, English
    [Refereed]
    Scientific journal

  • Tomohisa Katsuda, Masayuki Azuma, Jyoji Kato, Susumu Takakuwa, Hiroshi Ooshima
    Ethanolamine was examined as a nitrogen source in the production of hydrogen by Rhodobacter capsulatus ST-410, a hydrogenase-deficient mutant of the strain B-100. It was found that ethanolamine supports cell growth as the sole nitrogen source and permits a large amount of hydrogen evolution, detected at 138 μmol/ml- culture from 3.5 mM ethanolamine and 30 mM DL-malate. The amount corresponded to a stoichiometric yield of 77% and was close to that obtained from 7.0 mM L-glutamate and 30 mM DL-malate. The hydrogen evolution rate per unit biomass (cells) was higher than that with L-glutamate, and the cells grown with ethanolamine had higher nitrogenase activity than the cells grown with L-glutamate. In terms of bioconversion of cellulosic and hemicellulosic biomass to hydrogen, D-glucose, D-xylose, and D-cellobiose were tested as substrates. The results indicated that those sugars permit a large evolution of hydrogen through cultivation with ethanolamine as a nitrogen source. For instance, the cells grown with 3.5 mM ethanolamine evolved hydrogen of 289 μmol/ml-culture (80% yield) from 30 mM D-glucose under a controlled pH of 6.4 to 6.9. © 2000, Taylor & Francis Group, LLC. All rights reserved.
    Jan. 2000, Bioscience, Biotechnology and Biochemistry, 64(2) (2), 248 - 253, English
    [Refereed]
    Scientific journal

  • Light distribution model for the externally illuminated cylindrical photo-bioreactor and its application for production of hydrogen by Rhodobacter capsulatus
    T. Katsuda, T. Arimoto, K. Igarashi, M. Azuma, J. Kato, H. Ooshima
    Nov. 1999, The 5th Asia-Pacific Biochemical Engineering Conference 1999 (Phuket, Thailand, 1999), P-BP9
    [Refereed]

  • Hiroshi Ooshima, Susumu Takakuwa, Tomohisa Katsuda, Masaki Okuda, Takeshi Shirasawa, Masayuki Azuma, Jyoji Kato
    The characteristics of Rhodobacter capsulatus ST410, a mutant of the wild strain B100 lacking hydrogenase activity, were investigated from the viewpoint of hydrogen production. When 30 mM DL-malate and 7 mM L-glutamate were used as carbon and nitrogen sources, respectively, in an argon atmosphere, a specific hydrogen evolution rate of 0.14 ml/h/mg-dry cells was obtained at 6600 lx and 33℃. The evolution rate strongly depended on the light intensity : the higher the light intensity, the larger the evolution rate became up to at least 6600 lx. R. capsulatus ST410 converted 60 mM malate to hydrogen at a yield of 68%, calculated as a percentage of the stoichiometric maximum for the complete conversion of the carbon source to H_2 and CO_2. On the other hand, when the wild strain was used under the same conditions, the yield was only 25%. R. capsulatus ST410 converted not only malate but also glucose and cellobiose to hydrogen with good yields(60% for 30mM glucose and 66% for 7.5 mM cellobiose). Ethanolamine was found to be a good nitrogen source, which permitted a large amount of hydrogen to be evolved and also depressed the cell growth to low levels.
    Elsevier BV, Jan. 1998, Journal of Fermentation and Bioengineering, 85(5) (5), 470 - 475, English
    [Refereed]
    Scientific journal

  • Hydrogen Detectable Color Indicator for Screening of Microorganisms
    T. Katsuda, H. Ooshima, M. Azuma, J. Kato
    Feb. 1997, Kagaku Kogaku Symposium Series 57 Horizon of Biochemical Engineering, 57, 81 - 85, English
    Symposium

■ MISC
  • CCSと微細藻類による有用物質生産
    勝田知尚
    Feb. 2008, 生物工学会誌, 86(2) (2), 84 - 84, Japanese
    Introduction scientific journal

  • 光合成微生物による水素生産のゆくえ
    勝田知尚
    日本生物工学会, Mar. 2002, 生物工学会誌, 80(3) (3), 128 - 128, Japanese
    Introduction scientific journal

■ Books And Other Publications
  • Production of antibody fragments in Escherichia coli. In: Patrick Chames (ed.), Antibody Engineering: Methods and Protocols, Second Edition, Method in Molecular Biology, vol. 907, pp. 305-324 (Chapter 18)
    KATSUDA Tomohisa, SONODA Hiroyuki, KUMADA Yoichi, YAMAJI Hideki
    Others, Springer Science+Business Media, Aug. 2012, English
    Scholarly book

  • 抗体医薬のための細胞構築と培養技術
    KATSUDA Tomohisa
    Others, CMC Publishing, Jun. 2010, Japanese
    Scholarly book

  • Frontier of Eco-Bio Energy
    KATSUDA Tomohisa, OOSHIMA Hiroshi
    Joint work, シーエムシー出版, Sep. 2005, Japanese
    Scholarly book

■ Lectures, oral presentations, etc.
  • Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix
    Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji
    SCEJ 55th Autumn Meeting, Sep. 2024, English, Sapporo
    [Invited]
    Invited oral presentation

  • Efficient transfection of insect cells with controlling cell cycle progression
    Hikaru Mori, Ryo Iwanaga, Yuki Honjo, Hideki Yamaji, Tomohisa Katsuda
    SCEJ 89th Annual Meeting, Mar. 2024, Japanese
    Poster presentation

  • Production of influenza virus-like particles displaying heterologous proteins
    Shimizu K, Matsuda T, Katsuda T, Yamaji H
    化学工学会第54回秋季大会, Sep. 2023, English
    Poster presentation

  • 昆虫細胞を用いたキメラインフルエンザウイルス様粒子の生産
    清水 康平, 市川 祐大, 松田 拓也, 勝田 知尚, 山地 秀樹
    化学工学会第88年会, Mar. 2023
    Poster presentation

  • 培養温度と光強度を概日周期で制御したヘマトコッカス プルビアリスによるアスタキサンチン生産
    勝田 知尚, 奥村 魁, 山地 秀樹
    化学工学会第88年会, Mar. 2023
    Poster presentation

  • An investigation of diffusion and adsorption behavior in protein A media
    Kohei HAYASHI, Tomohisa KATSUDA, Hideki YAMAJI
    化学工学会第87年会, Mar. 2022, English
    Poster presentation

  • 異種タンパク質を提示するインフルエンザウイルス様粒子の昆虫細胞を用いた生産
    市川 裕大, 松田 拓也, Prihardi Kahar, 勝田 知尚, 山地 秀樹
    化学工学会第87年会, Mar. 2022, English
    Poster presentation

  • 分離精製（膜・クロマト分離）
    勝田 知尚
    日本生物工学会100周年記念事業教育セミナー「培養技術勉強会」, Nov. 2021, Japanese
    [Invited]
    Public discourse

  • 遺伝子組換え昆虫細胞樹立へのゲノム編集技術の応用
    松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹
    化学工学会第52回秋季大会, Sep. 2021

  • ゲノム編集技術を用いた遺伝子組換え昆虫細胞の樹立
    松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹
    第34回日本動物細胞工学会2021年度大会 (JAACT2021), Jul. 2021

  • プロテインA固定化マイクロアフィニティカラムを用いた疑似オンラインIgG濃度測定法の開発
    川村 和樹, 勝田 知尚, 山地 秀樹
    化学工学会第86年会, Mar. 2021, Japanese
    Poster presentation

  • Production of influenza virus-like particles using recombinant insect cells
    Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    The 33rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2020 Fuchu), Nov. 2020

  • 昆虫細胞を用いたインフルエンザウイルス様粒子の生産
    松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹
    化学工学会第51回秋季大会, Sep. 2020, Japanese

  • Effects of the circadian control in temperature and light intensity on the cell division of green algae under a photoautotrophic condition
    Tomohisa KATSUDA, Hiroyuki YAMADA, Takaaki MORIMOTO, Hitoki KUWAI, Hideki YAMAJI
    Young Asian Biological Engineers' Community Symposium 2019, Nov. 2019, English, Young Asian Biological Engineers' Community, Seoul, Korea, Republic of
    Poster presentation

  • Adsorption of immunoglobulin G in protein A resins studied by confocal laser scanning microscopy taking account of the optical hindrance inside a particle
    Tomohisa Katsuda, Tomoki Yamaguchi, Naruaki Inoue, Yuki Kase, Kou Ota, Hideki Yamaj
    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English
    Poster presentation

  • Production of influenza virus proteins in recombinant insect cells
    Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English
    Poster presentation

  • 微細藻の細胞分裂に及ぼす培養温度・光強度の周期的制御の影響
    勝田 知尚, 森本 高章, 桑井 仁葵, 山地 秀樹
    第71回日本生物工学会大会, Sep. 2019, Japanese
    Oral presentation

  • 組換え昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産
    松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹
    第32回日本動物細胞工学会2019年度大会 (JAACT2019), Jul. 2019, Japanese
    Poster presentation

  • Production of influenza virus-like particles in insect cells
    Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda
    26th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2019), May 2019, English
    Poster presentation

  • 固定化したシトクロムP450発現大腸菌によるwhole cell bioconversion
    FUJII ATSUSHI, ISHISAKA Akiho, KATSUDA Tomohisa, IMAISHI Hiromasa, YAMAJI Hideki
    化学工学会第84年会, Mar. 2019, Japanese, 化学工学会, 東京, Domestic conference
    Poster presentation

  • Production of influenza virus-like particles in stably transformed insect cells
    MATSUDA Takuya, TANIJIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, 日本動物細胞工学会, 筑波, International conference
    Poster presentation

  • Production of antibody Fab fragment using 2A peptide in insect cells
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, 日本動物細胞工学会, 筑波, International conference
    Poster presentation

  • 緑藻Chlorella sorokinianaの光独立栄養培養における培養温度の影響
    KUWAI Hitoki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第50回秋季大会, Sep. 2018, Japanese, 化学工学会, 鹿児島, Domestic conference
    Poster presentation

  • 培養温度・光強度の周期的な変化が緑藻Haematococcus pluvialisの細胞分裂に及ぼす影響
    MORIMOTO Takaaki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第50回秋季大会, Sep. 2018, Japanese, 化学工学会, 鹿児島, Domestic conference
    Poster presentation

  • 昆虫細胞における2Aペプチドを用いた抗体生産
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会大会, 吹田, Domestic conference
    Oral presentation

  • ゲノム編集技術を用いた組換え昆虫細胞の作製
    TOGAMI Shinya, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第50回秋季大会, Sep. 2018, English, 化学工学会, 鹿児島, Domestic conference
    Poster presentation

  • Production of influenza virus proteins in stably transformed insect cells
    YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa
    18th European Congress on Biotechnology (ECB2018), Jul. 2018, English, European Federation of Biotechnology, Geneva, Switzerland, International conference
    Poster presentation

  • 緑藻Haematococcus pluvialisにおける周期的温度変化と細胞増殖の関係
    KATSUDA Tomohisa, MORIMOTO Takaaki, YAMAJI Hideki
    化学工学会第83年会, Mar. 2018, Japanese, 化学工学会, 吹田, Domestic conference
    Poster presentation

  • 昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産
    MATSUDA Takuya, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第83年会, Mar. 2018, Japanese, 化学工学会, 吹田, Domestic conference
    Poster presentation

  • 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • 共焦点レーザー走査顕微鏡観察と数値ミュレーションに基づく抗体の吸着・破過の解析
    HIROOKA Kanamu, YAMAGUCHI Tomoki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • エアリフト式フォトバイオリアクターの操作条件と微細藻類の光独立栄養増殖の関係
    KUREBAYASHI Aika, MORIMOTO Takaaki, NISHIMURA Takumi, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • Production of influenza virus proteins by recombinant insect cells
    YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa
    International Meeting & 42nd Annual Conference of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Aug. 2017, English, The Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Kuala Lumpur, Malaysia, International conference
    Poster presentation

  • 昆虫細胞を用いたインフルエンザウイルスタンパク質の生産
    YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa
    第30回日本動物細胞工学会2017年度大会 (JAACT2017), Jul. 2017, Japanese, 日本動物細胞工学会, 松山, Domestic conference
    Poster presentation

  • Efficient protein production by transient gene expression using insect cells
    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa
    25th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2017), May 2017, English, European Society for Animal Cell Technology (ESACT), Lausanne, Switzerland, International conference
    Poster presentation

  • Transient gene expression in insect cells for efficient recombinant antibody production
    TAKAHASHI Ryoma, MASUMI-KOIZUMI Kyoto, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conference
    Poster presentation

  • Production of virus-like particles using recombinant insect cells
    TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki
    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conference
    Poster presentation

  • Characterization of the diffusion and adsorption of IgG in protein A-coupled adsorbents with confocal laser scanning microscopy
    HIROOKA Kanamu, HARUNA Yusuke, YAMAJI Hideki, KATSUDA Tomohisa
    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conference
    Poster presentation

  • Efficient antibody production by transient gene expression in insect cells
    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa
    17th European Congress on Biotechnology (ECB2016), Jul. 2016, English, European Federation of Biotechnology (EFB), Krakow, Poland, International conference
    Poster presentation

  • 培養条件の概日変化が緑藻Haematococcus pluvialisの栄養増殖に及ぼす影響
    Kohei YOSHIMINE, YAMADA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第81年会, Mar. 2016, Japanese, 化学工学会, 大阪, Domestic conference
    Poster presentation

  • 共焦点レーザー走査顕微鏡観察に基づくProtein A固定化吸着剤の拡散・吸着特性の評価
    KATSUDA Tomohisa, HARUNA Yusuke, HIROOKA Kanamu, KASE Yuki, OTA Kou, YOSHIKAWA Toru, YAMAJI Hideki
    化学工学会第81年会, Mar. 2016, Japanese, 化学工学会, 大阪, Domestic conference
    Oral presentation

  • Recombinant antibody production by transient gene expression in insect cells
    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa
    Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conference
    Poster presentation

  • Photoautotrophic growth of Haematococcus pluvialis under controlled temperature and light conditions
    KATSUDA Tomohisa, GIANNELLI Luca, YAMADA Hiroyuki, YAMAJI Hideki
    Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conference
    Poster presentation

  • Optimization of microalgal cultivation in cascade photobioreactors with wavy bottom via computational fluid dynamics
    WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa
    Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conference
    Poster presentation

  • シトクロムP450発現大腸菌を用いたwhole cell bioconversionに及ぼす培養・反応条件の影響
    HARADA Masayuki, MORINAKA Ryohei, KATSUDA Tomohisa, IMAISHI Hiromaisa, YAMAJI Hideki
    化学工学会第47回秋季大会, Sep. 2015, Japanese, 化学工学会, 札幌, Domestic conference
    Poster presentation

  • 昆虫細胞を宿主とした一過性発現による抗体タンパク質の生産
    MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    第28回日本動物細胞工学会2015年度大会 (JAACT2015), Jul. 2015, Japanese, 日本動物細胞工学会, 仙台, Domestic conference
    Poster presentation

  • Production of an antibody molecule by transient gene expression in insect cells
    MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    24th ESACT Meeting Barcelona 2015, Jun. 2015, English, European Society for Animal Cell Technology (ESACT), Barcelona, Spain, International conference
    Poster presentation

  • 鉛に汚染された土壌の評価とバイオレメディエーションによる鉛の回収
    塩見 尚史, SUETSUGU KENICHIRO, KATSUDA TOMOHISA
    日本ＬＣＡ学会, Mar. 2015, Japanese, Domestic conference
    Oral presentation

  • カスケード型フォトバイオリアクターにおける微細藻類培養の数値流体力学に基づく検討
    WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会姫路大会, Dec. 2014, Japanese, 化学工学会関西支部, 姫路, Domestic conference
    Poster presentation

  • Transient gene expression in insect cells for recombinant antibody production
    MORI Keita, HAMADA Hirotsugu, OGAWA Takahumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    The 27th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT2014 Kitakyushu), Nov. 2014, English, 日本動物細胞工学会, 北九州, International conference
    Poster presentation

  • 昆虫細胞を宿主とした一過性発現による抗体タンパク質生産
    HAMADA Hirotsugu, MORI Keita, OGAWA Takafumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡, Domestic conference
    Poster presentation

  • Production of antibody fragments by transient gene expression in insect cells
    YAMAJI Hideki, KATSUDA Tomohisa, HAMADA Hirotsugu, MORI Keita
    16th European Congress on Biotechnology (ECB16), Jul. 2014, English, European Federation of Biotechnology, Edinburgh, UK, International conference
    Poster presentation

  • 昆虫細胞を用いた一過性発現による機能性抗体タンパク質の生産
    MORI Keita, HAMAMOTO Kohei, MIYAGUCHI Naoko, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference
    Poster presentation

  • Haematococcus pluvialisの増殖とアスタキサンチン生産に及ぼす培養温度の影響
    YAMADA Hiroyuki, WADA Sotaro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference
    Poster presentation

  • Photobioreactor design using CFD: mixing and light effects on microalgal cultures
    GIANNELLI Luca, WADA Sotaro, YAMADA Hiroyuki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第78年会, Mar. 2013, English, 化学工学会, 豊中市, Domestic conference
    Oral presentation

  • Production of recombinant antibody molecules in insect cells
    MIYAGUCHI Naoko, HAMAMOTO Kouhei, OGAWA Takafumi, SONODA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki
    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, English, 日本動物細胞工学会, 名古屋市, International conference
    Poster presentation

  • Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells
    HOTTA Yuri, MINAMITANI Azusa, NARITA Kazuma, KATSUDA Tomohisa, YAMAJI Hideki
    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, English, 日本動物細胞工学会, 名古屋市, International conference
    Poster presentation

  • 組換え昆虫細胞によるscFv-Fc融合タンパク質の生産
    SONODA Hiroyuki, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki
    第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference
    Oral presentation

  • Computational fluid dynamics for enhancing the light distribution in a photobioreactor grown culture of Hematococcus pluvialis
    GIANNELLI Luca, WADA Sotaro, YAMAJI Hideki, KATSUDA Tomohisa
    第64回日本生物工学会大会, Oct. 2012, English, 日本生物工学会, 神戸市, Domestic conference
    Oral presentation

  • 大腸菌を用いた一本鎖抗体生産における終止コドンの影響
    YAMAJO Yuki, FUKUMARU Yuka, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第44回秋季大会, Sep. 2012, Japanese, 化学工学会, 仙台市, Domestic conference
    Poster presentation

  • 気液界面を利用した高収率リポソーム生成法の開発
    Hirohiko AIHARA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Ruri HIDEMA
    化学工学会第44回秋季大会仙台, Sep. 2012, Japanese, 仙台, Domestic conference
    Oral presentation

  • 昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産
    YAMAJI Hideki, SEGAWA Maiko, NAKAMURA Masataka, KATSUDA Tomohisa
    化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 東京, Domestic conference
    Oral presentation

  • 炭化水素資化酵母Yarrowia lipolyticaによる油汚染土壌の修復のための無機栄養源の開発
    FUJIMOTO Sho, TANUMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • 昆虫細胞を用いた抗体タンパク質の分泌生産
    HAMAMOTO Kouhei, MIYAGUCHI Naoko, SAWAMOTO Shiori, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • 昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討
    NARITA Kazuma, HOTTA Yuri, MINAMITANI Azusa, NAGASUGA Takashi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • シャイン・ダルガーノ配列を改変した大腸菌による一本鎖抗体の流加培養生産
    FUKUMARU Yuka, NOGAMI Tatsuhiro, KIHARA Mana, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    IUMS 2011 Sapporo Congress, Sep. 2011, English, International Union of Microbiological Societies, 札幌, Domestic conference
    Poster presentation

  • Secretory production of virus-like particles by recombinant insect cells
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    ESACT 2011 Meeting, May 2011, English, European Society for Animal Cell Technology, Vienna, Austria, International conference
    Poster presentation

  • 炭化水素資化酵母Yarrowia lipolyticaを用いた油汚染土壌の修復における栄養源の添加条件の検討
    FUJIMOTO Shou, TANUMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki
    第13回化学工学会学生発表会(神戸大会), Mar. 2011, Japanese, 化学工学会, 神戸市, Domestic conference
    Oral presentation

  • 大腸菌を用いた一本鎖抗体生産におけるシャイン・ダルガーノ配列の影響
    FUKUMARU Yuka, NOGAMI Tatsuhiro, KATSUDA Tomohisa, YAMAJI Hideki
    第13回化学工学会学生発表会(神戸大会), Mar. 2011, Japanese, 化学工学会, 神戸市, Domestic conference
    Oral presentation

  • 大腸菌におけるインクルージョンボディ生成に及ぼすシャイン・ダルガーノ配列の影響
    KATSUDA Tomohisa, FUKUMARU Yuka, KIHARA Mana, NOGAMI Tatsuhiro, YAMAJI Hideki
    化学工学会第76年会, Mar. 2011, Japanese, 化学工学会, 東京都, Domestic conference
    Oral presentation

  • リポソームの応用事例
    KATSUDA Tomohisa
    近畿バイオインダストリー振興会議第5回フォローアップ勉強会, Jan. 2011, Japanese, 近畿バイオインダストリー振興会議, 大阪市, Domestic conference
    Invited oral presentation

  • Optimized Shine-Dalgarno sequence for efficient production of single-chain Fv antibody in Escherichia coli
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), Dec. 2010, English, Canadian Society for Chemistry, American Chemical Society, Chemical Society of Japan, New Zealand Institute of Chemistry, Royal Australian Chemical Institute, Korean Chemical Society, Chinese Chemical Society, Honolulu, USA, International conference
    Poster presentation

  • Rapid production of functional antibody molecules by transient gene expression in insect cells
    SAWAMOTO Shiori, HAMAMOTO Kouhei, KATSUDA Tomohisa, YAMAJI Hideki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, English, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, International conference
    Poster presentation

  • Effect of signal peptide on the production of single-chain fv antibody by Escherichia coli
    NOGAMI Tatsuhiro, FUKUMARU Yuka, KATSUDA Tomohisa, YAMAJI Hideki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, English, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, International conference
    Poster presentation

  • Production of Japanese encephalitis virus-like particles in insect cell expression systems
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010), Oct. 2010, English, Asia Pacific Confederation of Chemical Engineering, Taipei, Taiwan, International conference
    Poster presentation

  • マイクロ流路内におけるリポソーム生成時のマイクロ流体挙動に関する研究
    FUJIWARA Jun, SUZUKI Hiroshi, KOMODA Hiroyuki, KATSUDA Tomohisa
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市
    Oral presentation

  • プロテインAアフィニティクロマトグラフィーのための溶離条件検討法の開発
    KASE Yuki, NISHIYAMA Takuya, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conference
    Poster presentation

  • シャペロン共発現による大腸菌での一本鎖抗体 (scFv) 融合タンパク質の生産
    SONODA Hiroyuki, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conference
    Poster presentation

  • アルカン資化酵母Yarrowia lipolyticaを用いた土壌汚染油の分解
    TANUMA Naoki, FUJIMOTO Shou, SHIOMI Naofumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conference
    Poster presentation

  • Production of Japanese encephalitis virus-like particles using insect cells
    NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, YAMAJI Hideki
    The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010), Sep. 2010, English, The Japanese Association for Animal Cell Technology, 札幌市, International conference
    Poster presentation

  • Pichia pastorisを用いた一本鎖抗体生産における誘導初期のメタノール濃度の影響
    FUJITA Keisuke, AKIYAMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第75年会, Mar. 2010, Japanese, 化学工学会, 鹿児島, Domestic conference
    Oral presentation

  • 昆虫細胞-バキュロウイルス系を用いた抗体タンパク質の高効率生産
    SAWAMOTO Shiori, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki
    第8回最先端バイオテクノロジー発表会, Feb. 2010, Japanese, 化学工学会, 大阪, Domestic conference
    Poster presentation

  • 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    第8回最先端バイオテクノロジー発表会, Feb. 2010, Japanese, 化学工学会, 大阪, Domestic conference
    Poster presentation

  • Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli
    KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2009, Dec. 2009, English, Young Asian Biochemical Engineers Community, Xiamen, International conference
    Poster presentation

  • Production of Japanese encephalitis virus-like particles by recombinant insect cells
    YAMAJI Hideki, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC '09), Nov. 2009, English, APBioChEC '09 Committee, 神戸市, International conference
    Oral presentation

  • Oil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolytica
    FUKUI Mayu, SHIOMI Shinji, TANUMA Naoki, KATSUDA Tomohisa, SHIOMI Naofumi, YAMAJI Hideki
    The 9th Asia-Pacific Biochemical Engineering Conference 2009, Nov. 2009, English, The 9th Asia-Pacific Biochemical Engineering Conference 2009, Kobe, International conference
    Poster presentation

  • 大腸菌を用いた一本鎖抗体生産におけるSD配列の影響
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference
    Poster presentation

  • 昆虫細胞を用いた日本脳炎ウイルス様粒子の生産
    TAKAHASHI Yusuke, NAGASUGA Takashi, NAKAMURA Masataka, KATSUDA Tomohisa, YAMAJI Hideki
    第61回日本生物工学会大会, Sep. 2009, Japanese, 日本生物工学会, 名古屋市, Domestic conference
    Oral presentation

  • 昆虫細胞-バキュロウイルス系による抗体タンパク質の生産
    SAWAMOTO Shiori, SUZUKI Tasuku, FURUTA Takanori, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference
    Poster presentation

  • ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用
    IWASAKI Daiki, SENGA Itaru, YAMASHITA Takashi, TONAI Masahiro, OGINO Chiaki, SHIMIZU Nobuaki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference
    Poster presentation

  • 昆虫細胞を用いた日本脳炎ウイルスタンパク質の生産
    TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference
    Oral presentation

  • 一塩基変異を検出する昇温溶出アフィニティクロマトグラフィーに対するPCRの適用性
    KATSUDA Tomohisa, IMANISHI Makoto, KATOH Shigeo, YAMAJI Hideki
    化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference
    Oral presentation

  • マイクロ流路内におけるリポソーム生成機構
    FUJIWARA Jun, HAMAMURA Junya, SUZUKI Hiroshi, KATSUDA Tomohisa, KOMODA Hiroyuki, USUI Hiromoto
    化学工学会第74年会, Mar. 2009, 化学工学会, 横浜, Domestic conference
    Oral presentation

  • Production of adenovirus vector by 293 cells immobilized within porous biomass support particles
    MORISHITA Naoya, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki
    The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, English, 日本動物細胞工学会, 福岡市, International conference
    Poster presentation

  • Low multiplicity infection of 293 cells for adenovirus vector production
    MORISHITA Naoya, YAMADA Kentaro, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki
    The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, English, 日本動物細胞工学会, 福岡市, International conference
    Poster presentation

  • High efficiency expression of soluble scFv in E. coli by use of linkers containing rare codons
    KAJIHARA Hideyuki, SAKAN Yoshinobu, KIHARA Mana, KIKUCHI Yasufumi, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki, KATOH Shigeo
    The 14th Symposium of Young Asian Biochemical Engineers’ Community, Nov. 2008, English, Young Asian Biochemical Engineers’ Community, 東京都, International conference
    Poster presentation

  • Effects of the parameters of flashing light from blue emitting diodes on astaxanthin production by H. pluvialis
    SENGA Itaru, SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2008, Nov. 2008, English, Young Asian Biochemical Engineers Community, Tokyo, International conference
    Poster presentation

  • A biochemical engineering study of photo-bioproces
    KATSUDA Tomohisa
    The 2 nd SCEJ (Kansai-Branch)/SSCCI Joint International Conference on Chemical Engineering, Nov. 2008, English, SCEJ (Kansai-Branch) and SSCCI, Shanghai, China, International conference
    [Invited]
    Invited oral presentation

  • Secretory production of Japanese encephalitis virus E protein by recombinant insect cells
    TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki
    “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, English, Hanyang University, Ansan, Korea, Domestic conference
    Poster presentation

  • Detection of single nucleotide variation in amplified gene fragments using oligonucleotide-coupled affinity column
    KATSUDA Tomohisa, IMANISHI Makoto, YAMAJI Hideki, KATOH Shigeo
    “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, English, Hanyang University, Ansan, Korea, Domestic conference
    Poster presentation

  • 多孔性粒子に固定化した大腸菌を用いる有用物質生産
    JIN Na, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki
    化学工学会第40回秋季大会, Sep. 2008, Japanese, 化学工学会, 仙台市, Domestic conference
    Poster presentation

  • レアコドンリンカーによる大腸菌での可溶性scFvの高効率生産
    KIHARA Mana, SAKAN Yoshinobu, KAJIHARA Hideyuki, AKASAKA Sho, KATSUDA Tomohisa, KATOH Shigeo, YAMAJI Hideki
    化学工学会第40回秋季大会, Sep. 2008, Japanese, 化学工学会, 仙台市, Domestic conference
    Poster presentation

  • 微生物の光合成を利用した有用物質生産ーエネルギーと食品への活用ー
    勝田 知尚
    第108回微小光学研究会, Jul. 2008, Japanese
    [Invited]
    Public discourse

  • Liposome Formation Characteristics with a Micro-Reactor
    Jun-ya HAMAMURA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Shigeo KATO, Hiromoto USUI
    11th Liposome Research Days Conference, Jul. 2008, English, Yokohama, Japan, International conference
    Oral presentation

  • Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen species
    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Daiki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    American Society for Microbiology 108th General Meeting, Jun. 2008, English, American Society for Microbiology, Boston, USA, International conference
    Poster presentation

  • マイクロ流路を用いたリポソームの生成機構
    鈴木 洋, 浜村 隼矢, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基
    化学工学会 第73年会, Mar. 2008, Japanese, 静岡, Domestic conference
    Oral presentation

  • Enhancement of growth and astaxanthin production of Haematococcus pluvialis by using airlift photobioreactor
    Ranjbar Reza, Inoue Ryota, Iwasaki Daiki, Katsuda Tomohisa, Katoh Shigeo
    化学工学会第73年会, Mar. 2008, English, 化学工学会, 浜松, Domestic conference
    Oral presentation

  • ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化
    SENGA Itaru, YAMAGAMI Keisuke, KATSUDA Tomohisa, OGINO Chiaki, SHIMIZU Nobuaki, KATOH Shigeo
    第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference
    Oral presentation

  • シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価
    TAKEDA Naoki, KATSUDA Tomohisa, KATOH Shigeo, MIYAHARA Hiroyoshi, INOUE Masaki, NAKAMURA Shuji
    第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference
    Oral presentation

  • High efficiency production of astaxantin by autotrophic cultivation of Haematococcua pluvialis in column photobioreactors
    RANJBAR Reza, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo
    第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, English, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference
    Oral presentation

  • Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination
    SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo
    上海市化学化工学会・化学工学会関西支部 第1回若手研究交流会, Dec. 2007, English, 上海市化学化工学会、化学工学会関西支部, 大阪, International conference
    Poster presentation

  • 青色点滅光照射下でのH. pluvialisの培養に及ぼす光強度および周波数の影響
    SHIRAISHI Hironori, ISHIZU Naoki, SENGA Itaru, RANJBAR Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会山口大会, Nov. 2007, Japanese, 化学工学会中国四国支部, 宇部, Domestic conference
    Poster presentation

  • Application of PCR to Affinity Chromatography for SNP Detection
    KATSUDA Tomohisa, KATOH Shigeo
    The 6th Asia-Europe Biorecognition Engineering Society Conference, Nov. 2007, English, Asia-Europe Biorecognition Engineering Society, Taoyuan, Taiwan, International conference
    [Invited]
    Invited oral presentation

  • Oxidative treatment of H. pluvialis with the sonocatalytic reaction
    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Hiroki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conference
    Poster presentation

  • Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination
    SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conference
    Poster presentation

  • A Biochemical Engineering Study of Bioproductions using Photobioreactor
    KATSUDA Tomohisa
    2007 Fall KSBB Meeting & International Symposium, Oct. 2007, English, The Korean Society for Biotechnology and Bioengineering, Daegu, Korea, International conference
    [Invited]
    Invited oral presentation

  • 二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導
    KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference
    Oral presentation

  • 気泡塔型フォトバイオリアクターを用いたヘマトコッカス藻による効率的アスタキサンチン生産
    INOUE Ryota, Ranjbar Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第39回秋季大会, Sep. 2007, Japanese, 化学工学会, 札幌, Domestic conference
    Poster presentation

  • マイクロキャピラリー内におけるリポソーム生成特性に関する研究
    濱村 隼矢, 鈴木 洋, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基
    化学工学会 第39回秋季大会, Sep. 2007, Japanese, 北海道大学, Domestic conference
    Oral presentation

  • フォトバイオリアクターによる 有用物質生産のための生物化学工学的検討
    KATSUDA Tomohisa
    第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference
    [Invited]
    Others

  • Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasounds
    KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    13th European Congress on Biotechnology, Sep. 2007, English, European Federation of Biotechnology, Barcelona, Spain, International conference
    Poster presentation

  • DNAアフィニティカラムを用いるSNP検出法へのPCR増幅法の応用
    IMANISHI Makoto, NISHIWADA Yasushi, FUKUI Mayu, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第39回秋季大会, Sep. 2007, Japanese, 化学工学会, 札幌, Domestic conference
    Poster presentation

  • 青色点滅光照射下のH. pluvialisにおけるアスタキサンチン生産量と光強度との関係
    SHIRAISHI Hironori, YAMAGAMI Keisuke, RANJBAR Reza, SENGA Itaru, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference
    Oral presentation

  • メタノール資化性酵母を用いた一本鎖抗体生産におけるメタノール供給方法
    OHNISHI Yuka, YAMAWAKI Shinya, MATSUMOTO Takehiro, SHIOMI Naofumi, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference
    Oral presentation

  • シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価
    TAKEDA Naoki, IMADA Masami, KATSUDA Tomohisa, KATOH Shigeo, MIYAHARA Hiroyoshi, INOUE Masaki, NAKAMURA Shuji
    化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference
    Oral presentation

  • Optimization of silica based media for antibody purification by protein A affinity chromatography
    IMADA Masami, TAKEDA Naoki, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, English, Young Asian Biochemical Engineers Community, Kaohsiung, International conference
    Poster presentation

  • Effects of flashing light from blue LEDs on the cell growth and astaxanthin production of Haematococcus pluvialis
    YAMAGAMI Keisuke, SHIRAISHI Hironori, RANJBARDAVIJANI Reza, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, English, Young Asian Biochemical Engineers Community, Kaohsiung, International conference
    Poster presentation

  • Optimization of silica-based matrices for antibody purification by protein A affinity chromatography
    KATOH Shigeo, IMADA Masami, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji
    26th international symposium on the separation of proteins, peptides and polynucleotides, Oct. 2006, English, ISPPP, Innsbruck, International conference
    Oral presentation

  • 昇温溶出アフィニティークロマトグラフィーによる一塩基多型検出法の開発
    KATSUDA Tomohisa
    化学工学会バイオ部会員フォーマルセミナー, Sep. 2006, Japanese, 化学工学会バイオ部会, 福岡, Domestic conference
    Oral presentation

  • メタノール資化性酵母Pichia pastorisの連続培養による一本鎖抗体生産
    YAMAWAKI Shinya, MATSUMOTO takehiro, OHNISHI Yuka, KATSUDA Tomohisa, SHIOMI Naofumi, KATOH Shigeo
    第58回生物工学会大会, Sep. 2006, Japanese, 生物工学会, 大阪, Domestic conference
    Oral presentation

  • ヘマトコッカスによるアスタキサンチン生産における青色点滅光照射の影響
    KATSUDA Tomohisa, SHIRAISHI Hironori, YAMAGAMI Keisuke, SENGA Itaru, SHIMAHARA Kazumichi, KATOH Shigeo
    第58回生物工学会大会, Sep. 2006, Japanese, 生物工学会, 大阪, Domestic conference
    Oral presentation

  • ヘマトコッカスによるアスタキサンチン生産における青色パルス光照射条件の検討
    SHIRAISHI Hironori, YAMAGAMI Keisuke, RANJBAR Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference
    Oral presentation

  • Pseudomonas sp. を用いる自己固定化バイオレメディエーションによるシアヌル酸分解
    SHIOMI Naofumi, ARII Kenji, NAKAI Hiroaki, YAMAGUCHI Yutaka, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference
    Oral presentation

  • Photocultivation of Haematococcus pluvialis Using Multi-Compartment Photobioreactor
    YOSHIMURA Satoshi, INOUE Ryota, RANJBER Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conference
    Oral presentation

  • SNP Detection Method Using Temperature Gradient Affinity Chromatography
    NISHIWADA Yasushi, KAMURA Mitsumasa, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conference
    Oral presentation

  • Flashing light Illumination for Effective Production of Astaxanthin by Haematococcus pluvialis
    SHIMAHARA Kazumichi, KATSUDA Tomohisa, YAMAGAMI Keisuke, SHIRAISHI Hironori, KATOH Shigeo
    化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conference
    Oral presentation

  • Effects of Iron Ion Concentration on the Growth Stability of Photosynthetic Bacteria in the Semi-Continuous Cultivation
    KATSUDA Tomohisa, YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, INOUE Ryota, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Oral presentation

  • Bioremediation of Acidified Pond and Soil
    SHIOMI Naofumi, NABESHIMA Risako, YASUDA Takako, IWASAKI Saori, YAMAGUCHI Yutaka, NAKAI Hiroaki, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Oral presentation

  • Effects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatus
    YOSHIMURA Satoshi, YEGANI Reza, MORIYA Kazunori, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Poster presentation

  • Detection of Single Nucleotide Polymorphysms by Affinity Chromatography with DNA Coupled Column
    NISHIWADA Yasushi, KAMURA Mitsumasa, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Poster presentation

  • Bioremediation of Herbicide-Polluted Soil with Self-Immobilized Microorganisms
    NAKAI Hiroaki, YAMAGUCHI Yutaka, YASUDA Takako, ARII Kenji, SAKAI Toyohide, KATSUDA Tomohisa, KATOH Shigeo, SHIOMI Naofumi
    平成17年度日本生物工学会大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conference
    Oral presentation

  • Rapid SNP Detection by Temperature Gradient Affinity Chromatography
    KATOH Shigeo, KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi
    25th International Symposium on the Separation of Proteins, Peptides & Polynucleotides, Nov. 2005, English, International Symposium on the Separation of Proteins, Peptides & Polynucleotides, St. Pete Beach, Florida, International conference
    Oral presentation

  • Effective Production of Astaxanthin from Haematococcus pluvialis by Use of Light Emitting Diodes
    SHIMAHARA Kazumichi, YAMAGAMI Keisuke, SHIRAISHI Hironori, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Poster presentation

  • Detection of SNP Using Affinity Chromatography of Immobilized Oligonucleotide
    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2005, Oct. 2005, English, Young Asia Biochemical Engineers Community, Beijing, China, International conference
    Oral presentation

  • Effects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatus
    KATSUDA Tomohisa, YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, INOUE Ryota, KATOH Shigeo
    化学工学会第37回秋季大会, Sep. 2005, Japanese, 化学工学会, 岡山, Domestic conference
    Poster presentation

  • Growth Sstability of Photosynthetic Bacteria : Effects of Supplemental Illumination and Ferrous Ion Concentration
    YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo
    The 12th European Congress on Biotechnology, Aug. 2005, English, European Federation of Biotechnology, Copenhagen, Denmark, International conference
    Poster presentation

  • Fed-Batch Cultivation of Haematococcus pluvialis under Illumination with LEDs for Production of Astaxanthin
    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo
    The 12th European Congress on Biotechnology, Aug. 2005, English, European Federation of Biotechnology, Copenhagen, Denmark, International conference
    Poster presentation

  • Temperature Gradient Elution of DNAs from an Oligo-DNA Immobilized Column
    KATSUDA Tomohisa, KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATOH Shigeo
    分離技術会年会2005, Jun. 2005, English, 分離技術会, 大阪, Domestic conference
    Poster presentation

  • Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis
    KATSUDA Tomohisa, LABABPOUR Abdolmajid, SHIMAHARA Kazumichi, KATOH Shigeo
    Asia-Pacific Biochemical Engineering Conference 2005, May 2005, English, The Korean Society for Biotechnology and Bioengineering, The Korean Institute of Chemical Engineers, Jeju, Korea, International conference
    Poster presentation

  • Detection of SNP using Affinity Chromatography of Immobilized Oligonucleotide
    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo
    The 4th Asia-Europe Biorecognition Engineering Society Conference, May 2005, English, Asia-Europe Biorecognition Engineering Society, Ansan, Korea, International conference
    Oral presentation

  • Effects of Nutrients Feeding Method on Astaxanthin Production by Haematococcus pluvialis
    KATSUDA Tomohisa, LABABPOUR Abdolmajid, KYOUI Yoshiaki, SHIMAHARA Kazumichi, KATOH Shigeo
    化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference
    Oral presentation

  • Elution Behavior of DNA from Oligo-DNA Immobilized Column under Temperature Gradient
    KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference
    Oral presentation

  • Astaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps
    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo
    2004 AIChE Annual Meeting, Nov. 2004, English, American Institute of Chemical Engineers, Austin, TX, International conference
    Oral presentation

  • Development of Oligonucleotide Immobilized Capillary Chromatography
    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, HOSSAIN Zakir, KATOH Shigeo
    The 2nd International Conference on Bioseparation Engineering, Oct. 2004, English, International conference
    Oral presentation

  • フォトバイオリアクターにおける光強度分布と増殖速度安定性の工学的検討
    イエガニ レザ, 吉村 誠司, 森谷 一徳, 勝田 知尚, 加藤 滋雄
    日本生物工学会平成16年度大会, Sep. 2004, Japanese, 日本生物工学会, 名城大学, Domestic conference
    Oral presentation

  • オリゴDNA固定化担体を用いた一塩基多型（SNP）の検出法の検討
    西島 研, 嘉村 光真, 勝田 知尚, 加藤 滋雄
    日本生物工学会平成16年度大会, Sep. 2004, Japanese, 日本生物工学会, 名城大学, Domestic conference
    Oral presentation

  • Instability of Growth Rate in Dense Culture of Photosynthetic Cells
    YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, English, Young Asian Biochemical Engineers' Community (YABEC), Osaka, Japan, International conference
    Oral presentation

  • Induction of Astaxanthin Accumulation in H. pluvialis by IIIumination with LED Lamps and Fed Batch Addition of Nutrients
    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, English, Young Asian Biochemical Engineers' Community (YABEC), Osaka, Japan, International conference
    Oral presentation

  • Growth and Astaxanthin Accumulation in H. pluvialis Cultures Illuminated by LED Lamps
    Lababpour Abdolmajid, 羽田 圭司, 島原 一道, 勝田 知尚, 加藤 滋雄
    化学工学会第69年会, Apr. 2004, English, 化学工学会, 大阪府立大学, Domestic conference
    Oral presentation

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産におよぼす青色パルス光の影響
    嶋原 一道, LABABPOUR Abdolmajid, 羽田 圭志, 勝田 知尚, 加藤 滋雄
    第55回日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本大学, Domestic conference
    Oral presentation

  • 光合成微生物の半連続培養における増殖安定性におよぼす光強度分布の影響
    勝田 知尚, イエガニ レザ, 吉村 誠司, 藤井 信彰, 五十嵐 啓介, 加藤 滋雄
    第55回日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本大学, Domestic conference
    Oral presentation

  • パルス光照射下におけるヘマトコッカスによるアスタキサンチン生産
    勝田 知尚, LABABPOUR Abdolmajid, 嶋原 一道, 羽田 圭志, 加藤 滋雄
    化学工学会 第36回秋季大会, Sep. 2003, Japanese, 化学工学会, 東北大学, Domestic conference
    Oral presentation

  • The effects of light intensity distribution on the stability of growth rate of a photosynthetic microorganism Rhodobacter capsulatus
    YEGANI Reza, 藤井 信彰, 五十嵐 啓介, 吉村 誠司, 勝田 知尚, 加藤 滋雄
    化学工学会 第36回秋季大会, Sep. 2003, English, 化学工学会, 東北大学, Domestic conference
    Oral presentation

  • Cultivation of microalga in photobioreactors illuminated with LEDs
    KATOH Shigeo, LABABPOUR Abdolmajid, KATSUDA Tomohisa
    The 6th Italian Conference on Chemical and Process Engineering, Jun. 2003, English, 未記入, 未記入, International conference
    Oral presentation

  • Development of a new SNP detectable method by use of the immobilized oligonucleotide capillary chromatography
    Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Shigeo Katoh
    Asia-Europe Symposium on Biomolecular Recognition, May 2003, English
    Oral presentation

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産における光照射法の検討
    勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄
    化学工学会第68年会, Mar. 2003, Japanese
    Oral presentation

  • 二槽式バイオリアクターによる光合成微生物の生産性の向上
    藤井 信彰, 五十嵐 啓介, Reza Yegani, 勝田 知尚, 加藤 滋雄
    化学工学会第68年会, Mar. 2003, Japanese
    Oral presentation

  • Astaxanthin production by Haematococcus pluvialis using LED lamps
    Tomohisa Katsuda, Abdolmajid Lababpour, Kazumichi Shimahara, Shigeo Katoh
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2002, Nov. 2002, English
    Oral presentation

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産に及ぼす照射光の影響
    Abdolmajid Lababpour, 嶋原 一道, 勝田 知尚, 加藤 滋雄
    化学工学会第35回秋季大会, Sep. 2002, English
    Oral presentation

  • 発光ダイオードを用いた緑藻Haematococcus pluvialisによるアスタキサンチン生産
    勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄
    化学工学会第35回秋季大会, Sep. 2002, Japanese
    Oral presentation

  • タンパク質のリフォールディングにおよぼす変性剤除去の影響
    宇高 太郎, 神野 一也, M.D. Farshbaf, 勝田 知尚, 加藤 滋雄
    化学工学会第35回秋季大会, Sep. 2002, Japanese
    Oral presentation

  • 高効率土壌汚染浄化法を利用した酸性雨の中和
    安田 多賀子, 岩崎 沙織, 勝田 知尚, 塩見 尚史, 加藤 滋雄
    化学工学会鳥取大会, Jul. 2002, Japanese
    Oral presentation

  • 光合成微生物培養液中における光強度減衰モデルの適用性
    勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄
    化学工学会第67年会, Mar. 2002, Japanese
    Oral presentation

  • B. subtilisの自己固定化能力を利用した酸性雨の土壌中での中和
    安田 多賀子, 岩崎 沙織, 井上 洋子, 楠本 智子, 勝田 知尚, 塩見 尚史, 加藤 滋雄
    化学工学会第67年会, Mar. 2002, Japanese
    Oral presentation

  • Light attenuation in suspension of purple bacterium Rhodobacter capsulatus and green alga Chlamydomonas reinhardtii
    Tomohisa Katsuda, Nobuaki Fujii, Natsushi Takata, Shigeo Katoh
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2001, Oct. 2001, English
    Oral presentation

  • 光合成微生物培養液中における光強度減衰
    勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄
    日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 2001, Japanese
    Oral presentation

  • 光合成微生物培養液中での光強度減衰に及ぼす光源の影響
    勝田 知尚, 藤井 信彰, 加藤 滋雄
    化学工学会福井大会, Jul. 2001, Japanese
    Oral presentation

  • 光合成微生物培養液中での光強度減衰
    勝田 知尚, 藤井 信彰, 大嶋 寛, 加藤 滋雄
    化学工学会第66年会, Apr. 2001, Japanese
    Oral presentation

  • テーラー渦型反応器によるタンパク質の連続リフォールディング
    加藤 芳寛, M.D. Farshbaf, 宇高 太郎, 野田 秀夫, 勝田 知尚, 加藤 滋雄
    化学工学会第66年会, Apr. 2001, Japanese
    Oral presentation

  • 円筒型光リアクターの新規な光強度分布モデルと光合成細菌による水素生産への適用
    勝田 知尚, 有本 武司, 五十嵐 幸一, 東 雅之, 大嶋 寛
    化学工学会第32回秋季大会, Sep. 1999, Japanese
    Oral presentation

  • 円筒型フォトバイオリアクターの新規な光強度分布モデルと光合成微生物による水素生産への適用
    勝田 知尚, 大嶋 寛
    日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1999, Japanese
    Oral presentation

  • エタノールアミンを窒素源として用いた光合成細菌Rhodobacter capsulatusによる水素生産
    勝田 知尚, 大嶋 寛
    日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1998, Japanese
    Oral presentation

  • エタノールアミンを窒素源として用いた、Rhodobacter capsulatusによる水素生産
    勝田 知尚, 大嶋 寛, 東 雅之, 加藤 錠治, 高桑 進
    平成9年度日本生物工学会大会, Sep. 1997, Japanese
    Oral presentation

  • Hydrogen detectable color indicator for screening of microorganisms
    Tomohisa Katsuda, Hiroshi Ooshima, Masayuki Azuma, Jyoji Kato
    化学工学会第29回秋季大会, Sep. 1996, English
    Poster presentation

■ Affiliated Academic Society
  • European Federation of Biotechnology
    May 2007 - Present

  • Asian Federation of Biotechnology

  • 日本生物工学会

  • 化学工学会

■ Research Themes
  • 培養温度制御に基づく真核細胞への遺伝子導入の効率化
    勝田 知尚
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2021 - 31 Mar. 2024
    本研究では，研究代表者らが緑藻の培養で見出した，培養温度を適切な範囲で周期的に変化させると培養液中の細胞間で細胞周期が同調するという知見に基づき，真核細胞の細胞周期を同調させることにより，核膜の消失から再構成の間にあわせて外来遺伝子を導入できるようにし，これによって外来遺伝子の核内移行，ならびに核DNAとの接触を促進させて，真核細胞への遺伝子導入の効率化を図ることを検討する． 令和3年度には，開放型の有糸分裂を行う昆虫細胞に注目し，培養温度をプログラム制御できるインキュベーターを用いて培養を行い，細胞周期が同調する温度制御条件の検討を行った．昆虫細胞には，組換えタンパク質の発現に広く利用されており，子宮頸がんワクチンの生産にも利用されているTrichoplusia ni BTI-TN-5B1-4 (High Five)細胞を用いた． はじめに，培養温度を最適よりも高温側で一定に保持して増殖挙動を調べた．High Five細胞は35℃までは最適温度である27℃のときと同等の増殖速度ならびに最高到達細胞密度を示した．しかし，37℃では増殖が顕著に抑制され，39℃では増殖しないことが分かった．培養液中のそれぞれの細胞の細胞周期をフローサイトメーターにより分析したところ，G2/M期にある細胞の全細胞数に対する割合が27℃の15－20%から37℃の30－40%まで，温度上昇に伴って増加することを見出した． そこで，培養温度は27℃と37℃を12 h周期で変化させることとし，High Five細胞の細胞周期の同調を試みた．しかし，植継ぎを行いながら6 dにわたって培養を継続したが，細胞周期が同調したときに見られる細胞密度の倍加は観察されなかった．High Five細胞では緑藻と同様にして細胞周期を同調させることは困難であることが分かった．

  • 勝田 知尚
    学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigator
    Competitive research funding

  • Production of influenza virus-like particles in recombinant insect cells
    Yamaji Hideki, KATSUDA Tomohisa
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2015 - 31 Mar. 2019
    Production of influenza virus-like particles in recombinant insect cells was investigated. The cDNA fragments encoding hemagglutinin (HA) and matrix protein 1 (M1) of an influenza virus A were separately cloned into the plasmid vector pIHAbla and pIHAneo. The pIHAbla and pIHAneo contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with either a blasticidin or neomycin resistance gene for use as a selectable marker, respectively. After cotransfection with the prepared plasmids, High Five cells were incubated with blasticidin and G418, and cells resistant to the antibiotics were effectively obtained. Sucrose density-gradient sedimentation analysis of the culture supernatant showed that secreted HA and M1 molecules were produced in a particulate form.

  • SUZUKI Hiroshi, KOMODA Yoshiyuki, KATSUDA Tomohisa
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2008 - 2010
    In order to investigate the formation mechanism of liposomes in a micro-channel, an experimental study has been performed. The Lagrangian observations and velocity field measurements by a micro-PTV were carried out by use of a movable stage. From the results, it was found that the lipid film pre-coated on the wall were taken into the liquid/air interface and the rod-like liposomes were formed between the interface and the wall and that the rod-like liposomes becomes spherical liposomes in a flow. It was also found that the rod-like liposomes serially induced from the interface by the strong reverse flow existing near the interface
    Competitive research funding

  • 産業技術研究「活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発」
    勝田 知尚
    2008, Principal investigator
    Competitive research funding

  • 昇温溶出アフィニティークロマトグラフィーによる一塩基多型の検出
    勝田 知尚
    2007, Principal investigator
    Competitive research funding

  • 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発
    勝田 知尚
    2006, Principal investigator
    Competitive research funding

  • 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発
    勝田 知尚
    2005, Principal investigator
    Competitive research funding

  • Integrate System for Creation, Detection and Production of Antibodies with Variety of Characteristics
    KATOH Shigeo, KATUSDA Tomohisa, SHIOMI Naofumi
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2003 - 2004
    In the fields of development of pharmaceuticals, medical diagnosis-analysis and bioprocesses, production of variety of antibodies, detection of the interactions between antibody and protein, screening methods of antibodies and effective production processes of antibodies are essential. In this work, we developed the simple and rapid methods to obtain antibody ligands for recognition and detection of target proteins, based on our recent researches on production of large libraries of antibodies, a high sensitivity detection method of proteins and effective production of antibodies by secretion from yeast cells. 1.A colony lift assay for detection of antibody secreted from E. coli was developed by use of antigen-adsorbed PVDF membrane and liposome immunoblotting assay. By use of this method, high density and micro-assay was possible and the number of samples treated was increased. 2.By use of HRP encapsulating immunoliposomes and hydrophobic membranes, an immunoblotting assay method with two orders of magnitude higher sensitivity, compared with the conventional immunoblotting assay using HRP-conjugated antibody, was developed. 3.A large library of whole antibodies was obtained based on mating of yeast cells transformed by libraries of H- and L-chains, and aproduction method of antibodies by secretion from yeast cells was established. 4.An affinity biopanning method utilizing immunoliposomes was developed and it was applied to biopanning selection with a high efficiency.

■ Industrial Property Rights
  • リポソームの製造方法
    SUZUKI HIROSHI, KOMODA YOSHIYUKI, KATSUDA TOMOHISA
    特願2012-553652, 06 Jan. 2012, 大学長, 特許5904555, 25 Mar. 2016
    Patent right

  • リポソーム製剤の製造方法
    SUZUKI HIROSHI, KATSUDA TOMOHISA, KATOH SHIGEO, KOMODA YOSHIYUKI, USUI HIROMOTO
    特願2007-134414, 21 May 2007, 大学長, 特許5126874, 09 Nov. 2012
    Patent right

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