Research activity information

• Sep. 2024 The Society of Chemical Engineers, Japan, Outstanding Paper Award of 2023, Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix

  Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji


• Nov. 2023 神戸大学工学部, 令和四年度優秀教育賞（応用化学科）

  勝田知尚


• Nov. 2018 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of influenza virus-like particles in stably transformed insect cells

  MATSUDA Takuya, TANIJIMA Toshikazu, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa, YAMAJI Hideki


• Oct. 2018 神戸大学工学部, 平成29年度優秀教育賞（応用化学科）

  勝田 知尚


• Sep. 2017 化学工学会, バイオ部会優秀ポスター賞, 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産

  MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki


• Nov. 2016 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of virus-like particles using recombinant insect cells

  TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki


• Oct. 2010 Asia Pacific Confederation of Chemical Engineering, Best Poster Paper Award (The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010)), Production of Japanese encephalitis virus-like particles in insect cell expression systems

  YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji


• Feb. 2010 化学工学会関西支部, 化学工学会バイオ部会, 第8回最先端バイオテクノロジー発表会ポスター賞, 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響, 野上達弘

  NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki


• Dec. 2009 Young Asian Biochemical Engineers’ Community, Best Poster Presentation Award for an Original Contribution to the 15th Symposium of Young Asian Biochemical Engineers’ Community, Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli

  KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki


• Sep. 2007 神戸大学工学部, 平成18年度優秀教育賞（移動現象論・分離工学分野）

  大村 直人, 今駒 博信, 鈴木 洋, 勝田 知尚


• Sep. 2007 日本生物工学会, 生物工学奨励賞(照井賞), フォトバイオリアクターによる有用物質生産のための生物化学工学的検討

  KATSUDA Tomohisa


• Mar. 2005 化学工学会, Masaki Naito Memorial Award, 光合成微生物による光培養生産プロセスの開発

  KATSUDA Tomohisa

• Using confocal laser scanning microscopy to analyze the mass transfer in the protein A medium of a silica matrix

  Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji

  Feb. 2023, Journal of Chemical Engineering of Japan, 56(1) (1), 2179369

  Link to Kobe Univ. Repository (Kernel)


• Effects of fluid-flow regimes on Chlorella sorokiniana cultivation in cascade photobioreactors with either flat or wavy bottoms

  Luca Giannelli, Chihiro Watanabe, Hideki Yamaji, Tomohisa Katsuda

  Nov. 2022, JOURNAL OF BIOTECHNOLOGY, 359, 15 - 20


• Effects of autophagy inducers on recombinant antibody production in insect cells

  Ryou Nakanuma, Kyoko Masumi-Koizumi, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji

  Jun. 2021, CYTOTECHNOLOGY, 73(3) (3), 299 - 305

  Link to Kobe Univ. Repository (Kernel)


• Production of influenza virus proteins using recombinant insect cells

  Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

  Jan. 2021, MATEC Web of Conferences, 333, 07009 - 07009


• Production of influenza virus-like particles using recombinant insect cells

  Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

  Nov. 2020, BIOCHEMICAL ENGINEERING JOURNAL, 163, 107757 - 107757

  Link to Kobe Univ. Repository (Kernel)


• Production of an antibody Fab fragment using 2A peptide in insect cells

  Yu Mizote, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

  Aug. 2020, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 130(2) (2), 205 - 211

  Link to Kobe Univ. Repository (Kernel)


• Production of influenza virus-like particles in insect cells

  Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda

  May 2020, BMC Proceedings, 14, 14 - 15


• Integration process for betacyanins extraction from peel and flesh of Hylocereus polyrhizus using liquid biphasic electric flotation system and antioxidant activity evaluation

  LEONG Hui Yi, OOI Chien Wei, LAW Chung Lim, JULKIFLE Advina Lizah, KATSUDA Tomohisa, SHOW Pau Loke

  Jan. 2019, Separation and Purification Technology, 209, 193 - 201


• Effects of lithium on the secretory production of recombinant antibody from insect cells

  OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki

  Jan. 2019, In Vitro Cellular & Developmental Biology - Animal, 55(1) (1), 1 - 6

  Link to Kobe Univ. Repository (Kernel)


• Surface grafting techniques on the improvement of membrane bioreactor: State-of-the-art advances

  LEE Xin Jiat, SHOW Pau Loke, KATSUDA Tomohisa, CHEN Wei-Hsin, CHANG Jo-Shu

  Dec. 2018, Bioresource Technology, 269, 489 - 502


• Production of influenza virus proteins in stably transformed insect cells

  YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa

  Oct. 2018, New Biotechnology, 44S, S159


• Green technology of liquid biphasic flotation for enzyme recovery utilizing recycling surfactant and sorbitol

  Revathy Sankaran, Pau Loke Show, Yee Jiun Yap, Yang Tao, Tau Chuan Ling, Katsuda Tomohisa

  Apr. 2018, Clean Technologies and Environmental Policy, 20(9) (9), 1 - 12


• Efficient protein production by transient gene expression using insect cells

  YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa

  Mar. 2018, BMC Proceedings, 12(3) (3), 19


• Efficient production of antibody Fab fragment by transient gene expression in insect cells

  Keita Mori, Hirotsugu Hamada, Takafumi Ogawa, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji

  Aug. 2017, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124(2) (2), 221 - 226

  Link to Kobe Univ. Repository (Kernel)


• Efficient antibody production by transient gene expression in insect cells

  Hideki Yamaji, Keita Mori, Hirotsugu Hamada, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda

  Jul. 2016, NEW BIOTECHNOLOGY, 33, S200 - S201


• Baculovirus display of functional antibody Fab fragments

  Shinya Takada, Takafumi Ogawa, Kazusa Matsui, Tasuku Suzuki, Tomohisa Katsuda, Hideki Yamaji

  Aug. 2015, CYTOTECHNOLOGY, 67(4) (4), 741 - 747


• Effects of temperature on the astaxanthin productivity and light harvesting characteristics of the green alga Haematococcus pluvialis

  Luca Giannelli, Hiroyuki Yamada, Tomohisa Katsuda, Hideki Yamaji

  Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 345 - 350


• A Numerical Model for the Quantification of Light/Dark Cycles in Microalgal Cultures: Air-Lift and Bubble-Column Photobioreactor Analysis by Means of Computational Fluid Dynamics

  Luca Giannelli, Hideki Yamaji, Tomohisa Katsuda

  Jan. 2015, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 48(1) (1), 61 - 71


• Production of an antibody fragment by transient gene expression in insect cells

  Hideki Yamaji, Tomohisa Katsuda, Hirotsugu Hamada, Keita Mori

  Jul. 2014, NEW BIOTECHNOLOGY, 31, S186 - S186


• Efficient production of Japanese encephalitis virus-like particles by recombinant lepidopteran insect cells

  Hideki Yamaji, Masataka Nakamura, Miwa Kuwahara, Yusuke Takahashi, Tomohisa Katsuda, Eiji Konishi

  Feb. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(3) (3), 1071 - 1079

  Link to Kobe Univ. Repository (Kernel)


• Production of Japanese encephalitis virus-like particles using the baculovirus-insect cell system

  Hideki Yamaji, Maiko Segawa, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi

  Dec. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(6) (6), 657 - 662


• Development of Liposome Preparation, Using Gas-Liquid Phase Adsorption

  Hirohiko AIHARA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Ruri HIDEMA

  Nov. 2012, International Workshop on Process Intensification 2012, 51 - 52


• Production of single-chain Fv-Fc fusion protein in stably transformed insect cells

  Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

  Aug. 2012, BIOCHEMICAL ENGINEERING JOURNAL, 67(-) (-), 77 - 82


• Production of antibody fragments in Escherichia coli.

  Tomohisa Katsuda, Hiroyuki Sonoda, Yoichi Kumada, Hideki Yamaji

  Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine-Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed.

  2012, Methods in molecular biology (Clifton, N.J.), 907, 305 - 24


• Optimization and Performance of Silica-Based Media for Industrial-Scale Antibody Purification

  Hiroyoshi Miyahara, Ryou Nakashima, Masaki Inoue, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

  Jan. 2012, CHEMICAL ENGINEERING & TECHNOLOGY, 35(1) (1), 157 - 160


• Effects of cytoplasmic and periplasmic chaperones on secretory production of single-chain Fv antibody in Escherichia coli

  Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

  Apr. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(4) (4), 465 - 470


• Cytoplasmic production of soluble and functional single-chain Fv-Fc fusion protein in Escherichia coli

  Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

  Feb. 2011, BIOCHEMICAL ENGINEERING JOURNAL, 53(3) (3), 253 - 259


• Efficient production of an antibody Fab fragment using the baculovirus-insect cell system

  Takanori Furuta, Takafumi Ogawa, Tomohisa Katsuda, Ikuo Fujii, Hideki Yamaji

  Nov. 2010, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 110(5) (5), 577 - 581


• Micro-fluidic behavior on liposome production in a micro-channel

  Atsushi Fujiwara, Hiroshi Suzuki, Tomohisa Katsuda, Yoshiyuki Komoda

  Oct. 2010, The 13th Asia Pacific Confederation of Chemical Engineering Congress Proceedings, 10338


• Production of Japanese encephalitis virus-like particles in insect cell expression systems

  YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

  Oct. 2010, The 13th Asia Pacific Confederation of Chemical Engineering Congress Proceedings, 10640


• Immobilization of 293 cells using porous support particles for adenovirus vector production

  Naoya Morishita, Tomohisa Katsuda, Shuji Kubo, Akinobu Gotoh, Hideki Yamaji

  Aug. 2010, CYTOTECHNOLOGY, 62(4) (4), 293 - 300


• Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones

  Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

  Apr. 2010, PROTEIN EXPRESSION AND PURIFICATION, 70(2) (2), 248 - 253


• Oil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolytica

  Mayu Fukui, Shinji Shiomi, Naoki Tanuma, Tomohisa Katsuda, Naofumi Shiomi, Hideki Yamaji

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S93 - S94


• Production of Japanese encephalitis virus-like particles by recombinant insect cells

  Hideki Yamaji, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(S1) (S1), S7 - S7


• Tandem multimer expression of angiotensin I-converting enzyme inhibitory peptide in Escherichia coli

  Hasan M. Fida, Yoichi Kumada, Masaaki Terashima, Tomohisa Katsuda, Shigeo Katoh

  Sep. 2009, Biotechnology Journal, 4(9) (9), 1345 - 1356


• Immobilization of Escherichia coli cells using polyethyleneimine-coated porous support particles for L-aspartic acid production

  Juan Huang, Na Jin, Tomohisa Katsuda, Hideki Fukuda, Hideki Yamaji

  Sep. 2009, BIOCHEMICAL ENGINEERING JOURNAL, 46(1) (1), 65 - 68


• Refolding of single-chain Fv by use of an antigen-coupled column

  Kazutaka Ikeda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

  May 2009, BIOCHEMICAL ENGINEERING JOURNAL, 44(2-3) (2-3), 289 - 291


• Adenovirus vector production using low-multiplicity infection of 293 cells

  Kentaro Yamada, Naoya Morishita, Tomohisa Katsuda, Shuji Kubo, Akinobu Gotoh, Hideki Yamaji

  Apr. 2009, CYTOTECHNOLOGY, 59(3) (3), 153 - 160


• Angiotensin-I Converting Enzyme (ACE) Inhibitory Mechanism of Tripeptides Containing Aromatic Residues

  Yuka Kobayashi, Tadashi Yamauchi, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

  Sep. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(3) (3), 310 - 312


• Size Characteristics of Liposomes Formed in a Micro-Tube

  Hiroshi Suzuki, Jun-ya Hamamura, Tomohisa Katsuda, Yoshiyuki Komoda, Sigeo Katoh, Hiromoto Usui

  Aug. 2008, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 41(8) (8), 739 - 743


• High Efficiency Production of Astaxanthin in an Airlift Photobioreactor

  Reza Ranjbar, Ryota Inoue, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

  Aug. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(2) (2), 204 - 207


• 微生物の光合成を利用した有用物質生産－エネルギーと食品への活用－

  勝田知尚

  Jul. 2008, Microoptics News, 26, 29 - 34


• High efficiency production of astaxanthin by autotrophic cultivation of Haematococcus pluvialis in a bubble column photobioreactor

  Reza Ranjbar, Ryota Inoue, Hironori Shiraishi, Tomohisa Katsuda, Shigeo Katoh

  May 2008, BIOCHEMICAL ENGINEERING JOURNAL, 39(3) (3), 575 - 580


• フォトバイオリアクターによる有用物質生産のための生物化学工学的検討

  KATSUDA Tomohisa

  日本生物工学会, Mar. 2008, 生物工学会誌, 86(3) (3), 117 - 122


• Effect of light intensity and frequency of flashing light from blue light emitting diodes on astaxanthin production by Haematococcus pluvialis

  Tomohisa Katsuda, Hironori Shiraishi, Naoki Ishizu, Reza Ranjbar, Shigeo Katoh

  Mar. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105(3) (3), 216 - 220


• Liposome formation characteristics in a micro-tube

  J. Hamamura, H. Suzuki, T. Katsuda, Y. Komoda, S. Katoh, H. Usui

  Nov. 2007, The 8th Asia-Pacific Biochemical Engineering Conference 2007 (Taipei, Taiwan, 2007), C0177


• Production of Single-Chain Variable Fragment Antibody (scFv) in Fed-Batch and Continuous Culture of Pichia pastoris by Two Different Methanol Feeding Methods

  Shinya Yamawaki, Takehiro Matsumoto, Yuka Ohnishi, Yoichi Kumada, Naofumi Shiomi, Tomohisa Katsuda, Eun Kyu Lee, Shigeo Katoh

  Nov. 2007, Journal of Bioscience and Bioengineering, 104(5) (5), 403 - 407


• Optimization of silica-based media for antibody purification by protein A affinity chromatography

  Shigeo Katoh, Masami Imada, Naoki Takeda, Tomohisa Katsuda, Hiroyoshi Miyahara, Masaki Inoue, Shuji Nakamura

  Aug. 2007, JOURNAL OF CHROMATOGRAPHY A, 1161(1-2) (1-2), 36 - 40


• ACE inhibitory activity and characteristics of tri-peptides obtained from bonito protein

  Md. Fida Hasan, Yuka Kobayashi, Yoichi Kumada, Tomohisa Katsuda, Masaaki Terashima, Shigeo Katoh

  Jan. 2007, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 40(1) (1), 59 - 62


• Application of regularized alternating least squares and independent component analysis to HPLC-DAD data of Haematococcus pluvialis metabolites

  Hiroyuki Yamamoto, Keishi Hada, Hideki Yamaji, Tomohisa Katsuda, Hiromu Ohno, Hideki Fukuda

  Dec. 2006, Biochemical Engineering Journal, 32(3) (3), 149 - 156


• Effect of flashing light from blue light emitting diodes on cell growth and astaxanthin production of Haematococcus pluvialis

  Tomohisa Katsuda, Kazumichi Shimahara, Hironori Shiraishi, Keisuke Yamagami, Reza Ranjbar, Shigeo Katoh

  Nov. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(5) (5), 442 - 446


• Elution behavior of DNAs in temperature-gradient affinity chromatography for single nucleotide polymorphism detection

  Tomohisa Katsuda, Mitsumasa Kamura, Yasushi Nishiwada, Shigeo Katoh

  Oct. 2006, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 39(10) (10), 1104 - 1107


• New detection method for hydrogen gas for screening hydrogen-producing microorganisms using water-soluble Wilkinson's catalyst derivative

  Tomohisa Katsuda, Hiroshi Oshima, Masayuki Azuma, Jyoji Kato

  Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 220 - 226


• Degradation of cyanuric acid in soil by Pseudomonas sp NRRL B-12227 using bioremediation with self-immobilization system

  Naofumi Shiomi, Yutaka Yamaguchi, Hiroaki Nakai, Tomoko Fujita, Tomohisa Katsuda, Shigeo Katoh

  Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 206 - 209


• Single nucleotide polymorphism detection method by temperature-gradient affinity chromatography using a single-stranded oligo-DNA coupled column

  Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Yasushi Nishiwada, Shigeo Katoh

  Aug. 2006, JOURNAL OF CHROMATOGRAPHY A, 1123(2) (2), 182 - 188

  Link to Kobe Univ. Repository (Kernel)


• Effective utilization of transmitted light for astaxanthin production by Haematococcus pluvialis

  Satoshi Yoshimura, Reza Ranjbar, Ryota Inoue, Tomohisa Katsuda, Shigeo Katoh

  Aug. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(2) (2), 97 - 101


• Fragmentation of angiotensin-I converting enzyme inhibitory peptides from bonito meat under intestinal digestion conditions and their characterization

  F. Hasan, Y. Kumada, N. Hashimoto, T. Katsuda, M. Terashima, S. Katoh

  Jun. 2006, FOOD AND BIOPRODUCTS PROCESSING, 84(C2) (C2), 135 - 138


• Improvement of growth stability of photosynthetic bacterium Rhodobacter capsulatus

  R Yegani, S Yoshimura, K Moriya, T Katsuda, S Katoh

  Dec. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100(6) (6), 672 - 677


• Fed-batch culture under illumination with blue light emitting diodes (LEDs) for astaxanthin production by Haematococcus pluvialis

  A Lababpour, K Shimahara, K Hada, Y Kyoui, T Katsuda, S Katoh

  Sep. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100(3) (3), 339 - 342


• Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis

  T. Katsuda, A. Lababpour, K. Shimahara, S. Katoh

  May 2005, The 7th Asia-Pacific Biochemical Engineering Conference 2005 (Jeju, Korea, 2005), P2-088


• Semi-Continuous Cultivation of Photosynthetic Cells in Flat Plate Photobioreactor

  YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo

  Apr. 2005, Iranian Journal of Chemical Engineering, 2(2) (2), 15 - 21


• Neutralization of acids by microorganisms co-immobilized with Bacillus subtilis in a shallow layer of model soil

  T Yasuda, N Shiomi, S Iwasaki, Y Yamaguchi, T Katsuda, S Katoh

  Dec. 2004, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 37(12) (12), 1445 - 1451


• Effects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis

  A Lababpour, K Hada, K Shimahara, T Katsuda, S Katoh

  Dec. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98(6) (6), 452 - 456


• Astaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps

  A. Lababpour, T. Katsuda, S. Katoh

  Nov. 2004, 2004 AIChE Annual Meeting Conference Proceedings, 483b


• Astaxanthin production by Haematococcus pluvialis under illumination with LEDs

  T Katsuda, A Lababpour, K Shimahara, S Katoh

  Jul. 2004, ENZYME AND MICROBIAL TECHNOLOGY, 35(1) (1), 81 - 86

  Link to Kobe Univ. Repository (Kernel)


• Effects of light intensity distribution on growth of Rhodobacter capsulatus

  T Katsuda, R Yegani, N Fujii, K Igarashi, S Yoshimura, S Katoh

  May 2004, BIOTECHNOLOGY PROGRESS, 20(3) (3), 998 - 1000


• Relationship between Light Intensity Distribution and Growth Rate of Photosynthetic Cells

  YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo

  From engineering point of view, the effect of light intensity distribution on the stability of growth rate can play an important role in designing of effective photobioreactors and sustaining of stable growth rates. In these series of experiments, in order to keep the operational parameters at almost constant level, a semi-continuous culture method was developed. In this cultivation method, a part of culture broth containing grown cells was repeatedly replaced with the fresh medium at a predetermined time interval to keep the cell concentration and the volume of the broth constant at initial values. Thus it was possible to cultivate photosynthetic cells under defined light intensity distributions.
  Under one side and both side illumination and also reflection by mirror photosynthetic cells were cultivated in flat plate photobioreactors with various light path lengths. The obtained results showed that stability of the growth rate strongly depended on the distribution of light intensity and ratio of light intensity at light zone to that at dark zone inside photobioreactors. These parameters should be taken into consideration for stable cultivation of photosynthetic cells.

  The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 3P-01-085, 807 - 807


• Effects of Medium Replacement on Cell Density and Astaxanthin Accumulation in H. pluvialis Illumination by LED Lamps

  Abdolmajid Lababpour, HADA Keishi, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo

  Haematococcus pluvialisis is one of the potential candidates for production of astaxanthin. Growth characteristics of H. pluvialis in batch run and medium replacement runs were measured. A two-stage process was employed for biomass and astaxanthin synthesis by this alga. During the first stage, medium was replaced several times every day to achieve high cell concentration. When the replacement was stopped, the light illumination was switched from red LEDs at 8μmol photons m^-2 s^-1 to blue LEDs at 12µmol photons m^-2 s^-1 to stimulate the accumulation of astaxanthin without decreasing in the cell numbers by death of the cells. H. pluvialis exhibited relatively high cell concentration in the first step and reached to about 1.5 mg-dry cell/cm³ and accumulated up to 65 µg/cm³ astaxanthin in the second stage, which are higher than those reported in literature.

  The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 3L-07, 430 - 430


• Detection of SNPs Using Oligonucleotide Immobilized Capillary Chromatography

  KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, Zakir Hossain, KATOH Shigeo

  In the present work, we investigated the effects of GC content, mismatching position, and length on the thermostability of double-strand oligo-DNAs in order to develop an affinity chromatography using oligonucleotide-immobilized capillary for SNP detection. We found that a single mismatching base pair appeared on 25 mer fragment of human tp53 tumor suppressor protein gene could reduce the melting temperature by 6.6°C. It seemed to be enough for eluting the mutated fragment separately from the intact one during a course of temperature ascending process.

  The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 1P-01-009, 479 - 479


• Characteristics of neutralization of acids by newly isolated fungal cells

  N Shiomi, T Yasuda, Y Inoue, N Kusumoto, S Iwasaki, T Katsuda, S Katoh

  Jan. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(1) (1), 54 - 58


• Cultivation of Microalga in Photobioreactors Illuminated with LEDs

  S. Katoh, A. Lababpour, T. Katsuda

  Jun. 2003, Chemical Engineering Transactions, 3, 1671 - 1675


• Characterization of continuous refolding apparatus using different mixing methods

  Mahmoud Farshbaf, Yoshihiro Katoh, Taro Udaka, Tomohisa Katsuda, Hideo Noda, Shigeo Katoh

  Oct. 2002, Journal of Chemical Engineering of Japan, 35(10) (10), 963 - 968


• Light attenuation in suspension of the purple bacterium Rhodobacter capsulatus and the green alga Chlamydomonas reinhardtii

  T Katsuda, N Fujii, N Takata, H Ooshima, S Katoh

  May 2002, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 35(5) (5), 428 - 435


• Control of aggregate formation in refolding of carbonic anhydrase at high urea concentrations and effects of urea removal

  M Farshbaf, Y Katoh, T Morimoto, T Udaka, T Katsuda, S Katoh

  Jun. 2001, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 34(6) (6), 743 - 747


• 光合成微生物の懸濁液中における光強度減衰

  勝田知尚, 加藤滋雄

  Apr. 2001, 神戸大学ベンチャー・ビジネス・ラボラトリー年報, 6, 185 - 190


• Light intensity distribution in the externally illuminated cylindrical photo-bioreactor and its application to hydrogen production by Rhodobacter capsulatus

  Tomohisa Katsuda, Takeshi Arimoto, Koichi Igarashi, Masayuki Azuma, Jyoji Kato, Susumu Takakuwa, Hiroshi Ooshima

  Jun. 2000, Biochemical Engineering Journal, 5(2) (2), 157 - 164


• Effects of Ethanolamine as a Nitrogen Source on Hydrogen Production by Rhodobacter capsulatus

  Tomohisa Katsuda, Masayuki Azuma, Jyoji Kato, Susumu Takakuwa, Hiroshi Ooshima

  Jan. 2000, Bioscience, Biotechnology and Biochemistry, 64(2) (2), 248 - 253


• Light distribution model for the externally illuminated cylindrical photo-bioreactor and its application for production of hydrogen by Rhodobacter capsulatus

  T. Katsuda, T. Arimoto, K. Igarashi, M. Azuma, J. Kato, H. Ooshima

  Nov. 1999, The 5th Asia-Pacific Biochemical Engineering Conference 1999 (Phuket, Thailand, 1999), P-BP9


• Production of hydrogen by a hydrogenase-deficient mutant of Rhodobacter capsulatus

  Hiroshi Ooshima, Susumu Takakuwa, Tomohisa Katsuda, Masaki Okuda, Takeshi Shirasawa, Masayuki Azuma, Jyoji Kato

  The characteristics of Rhodobacter capsulatus ST410, a mutant of the wild strain B100 lacking hydrogenase activity, were investigated from the viewpoint of hydrogen production. When 30 mM DL-malate and 7 mM L-glutamate were used as carbon and nitrogen sources, respectively, in an argon atmosphere, a specific hydrogen evolution rate of 0.14 ml/h/mg-dry cells was obtained at 6600 lx and 33℃. The evolution rate strongly depended on the light intensity : the higher the light intensity, the larger the evolution rate became up to at least 6600 lx. R. capsulatus ST410 converted 60 mM malate to hydrogen at a yield of 68%, calculated as a percentage of the stoichiometric maximum for the complete conversion of the carbon source to H_2 and CO_2. On the other hand, when the wild strain was used under the same conditions, the yield was only 25%. R. capsulatus ST410 converted not only malate but also glucose and cellobiose to hydrogen with good yields(60% for 30mM glucose and 66% for 7.5 mM cellobiose). Ethanolamine was found to be a good nitrogen source, which permitted a large amount of hydrogen to be evolved and also depressed the cell growth to low levels.

  Elsevier BV, Jan. 1998, Journal of Fermentation and Bioengineering, 85(5) (5), 470 - 475


• Hydrogen Detectable Color Indicator for Screening of Microorganisms

  T. Katsuda, H. Ooshima, M. Azuma, J. Kato

  Feb. 1997, Kagaku Kogaku Symposium Series 57 Horizon of Biochemical Engineering, 57, 81 - 85

• 外来タンパク質を提示したインフルエンザVLPsの昆虫細胞を用いた生産

  市川祐大, 松田拓也, PRIHARDI Kahar, 勝田知尚, 山地秀樹

  2022, Program & Abstracts. Annual and International Meeting of the Japanese Association for Animal Cell Technology, 35th (CD-ROM)


• CCSと微細藻類による有用物質生産

  勝田知尚

  Feb. 2008, 生物工学会誌, 86(2) (2), 84 - 84


• 光合成微生物による水素生産のゆくえ

  勝田知尚

  日本生物工学会, Mar. 2002, 生物工学会誌, 80(3) (3), 128 - 128

• Production of antibody fragments in Escherichia coli. In: Patrick Chames (ed.), Antibody Engineering: Methods and Protocols, Second Edition, Method in Molecular Biology, vol. 907, pp. 305-324 (Chapter 18)

  KATSUDA Tomohisa, SONODA Hiroyuki, KUMADA Yoichi, YAMAJI Hideki

  Springer Science+Business Media, Aug. 2012


• 抗体医薬のための細胞構築と培養技術

  KATSUDA Tomohisa

  CMC Publishing, Jun. 2010


• Frontier of Eco-Bio Energy

  KATSUDA Tomohisa, OOSHIMA Hiroshi

  シーエムシー出版, Sep. 2005

• Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix

  Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji

  SCEJ 55th Autumn Meeting, Sep. 2024, Sapporo


• Efficient transfection of insect cells with controlling cell cycle progression

  Hikaru Mori, Ryo Iwanaga, Yuki Honjo, Hideki Yamaji, Tomohisa Katsuda

  SCEJ 89th Annual Meeting, Mar. 2024


• Production of influenza virus-like particles displaying heterologous proteins

  Shimizu K, Matsuda T, Katsuda T, Yamaji H

  化学工学会第54回秋季大会, Sep. 2023


• 昆虫細胞を用いたキメラインフルエンザウイルス様粒子の生産

  清水 康平, 市川 祐大, 松田 拓也, 勝田 知尚, 山地 秀樹

  化学工学会第88年会, Mar. 2023


• 培養温度と光強度を概日周期で制御したヘマトコッカス プルビアリスによるアスタキサンチン生産

  勝田 知尚, 奥村 魁, 山地 秀樹

  化学工学会第88年会, Mar. 2023


• An investigation of diffusion and adsorption behavior in protein A media

  Kohei HAYASHI, Tomohisa KATSUDA, Hideki YAMAJI

  化学工学会第87年会, Mar. 2022


• 異種タンパク質を提示するインフルエンザウイルス様粒子の昆虫細胞を用いた生産

  市川 裕大, 松田 拓也, Prihardi Kahar, 勝田 知尚, 山地 秀樹

  化学工学会第87年会, Mar. 2022


• 分離精製（膜・クロマト分離）

  勝田 知尚

  日本生物工学会100周年記念事業教育セミナー「培養技術勉強会」, Nov. 2021


• 遺伝子組換え昆虫細胞樹立へのゲノム編集技術の応用

  松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹

  化学工学会第52回秋季大会, Sep. 2021


• ゲノム編集技術を用いた遺伝子組換え昆虫細胞の樹立

  松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹

  第34回日本動物細胞工学会2021年度大会 (JAACT2021), Jul. 2021


• プロテインA固定化マイクロアフィニティカラムを用いた疑似オンラインIgG濃度測定法の開発

  川村 和樹, 勝田 知尚, 山地 秀樹

  化学工学会第86年会, Mar. 2021


• Production of influenza virus-like particles using recombinant insect cells

  Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

  The 33rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2020 Fuchu), Nov. 2020


• 昆虫細胞を用いたインフルエンザウイルス様粒子の生産

  松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹

  化学工学会第51回秋季大会, Sep. 2020


• Effects of the circadian control in temperature and light intensity on the cell division of green algae under a photoautotrophic condition

  Tomohisa KATSUDA, Hiroyuki YAMADA, Takaaki MORIMOTO, Hitoki KUWAI, Hideki YAMAJI

  Young Asian Biological Engineers' Community Symposium 2019, Nov. 2019, Young Asian Biological Engineers' Community, Seoul


• Adsorption of immunoglobulin G in protein A resins studied by confocal laser scanning microscopy taking account of the optical hindrance inside a particle

  Tomohisa Katsuda, Tomoki Yamaguchi, Naruaki Inoue, Yuki Kase, Kou Ota, Hideki Yamaj

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019


• Production of influenza virus proteins in recombinant insect cells

  Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019


• 微細藻の細胞分裂に及ぼす培養温度・光強度の周期的制御の影響

  勝田 知尚, 森本 高章, 桑井 仁葵, 山地 秀樹

  第71回日本生物工学会大会, Sep. 2019


• 組換え昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産

  松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹

  第32回日本動物細胞工学会2019年度大会 (JAACT2019), Jul. 2019


• Production of influenza virus-like particles in insect cells

  Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda

  26th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2019), May 2019


• 固定化したシトクロムP450発現大腸菌によるwhole cell bioconversion

  FUJII ATSUSHI, ISHISAKA Akiho, KATSUDA Tomohisa, IMAISHI Hiromasa, YAMAJI Hideki

  化学工学会第84年会, Mar. 2019, 化学工学会, 東京


• Production of influenza virus-like particles in stably transformed insect cells

  MATSUDA Takuya, TANIJIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

  31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, 日本動物細胞工学会, 筑波


• Production of antibody Fab fragment using 2A peptide in insect cells

  MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

  31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, 日本動物細胞工学会, 筑波


• 緑藻Chlorella sorokinianaの光独立栄養培養における培養温度の影響

  KUWAI Hitoki, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第50回秋季大会, Sep. 2018, 化学工学会, 鹿児島


• 培養温度・光強度の周期的な変化が緑藻Haematococcus pluvialisの細胞分裂に及ぼす影響

  MORIMOTO Takaaki, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第50回秋季大会, Sep. 2018, 化学工学会, 鹿児島


• 昆虫細胞における2Aペプチドを用いた抗体生産

  MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

  第70回日本生物工学会大会, Sep. 2018, 日本生物工学会大会, 吹田


• ゲノム編集技術を用いた組換え昆虫細胞の作製

  TOGAMI Shinya, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第50回秋季大会, Sep. 2018, 化学工学会, 鹿児島


• Production of influenza virus proteins in stably transformed insect cells

  YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa

  18th European Congress on Biotechnology (ECB2018), Jul. 2018, European Federation of Biotechnology, Geneva, Switzerland


• 緑藻Haematococcus pluvialisにおける周期的温度変化と細胞増殖の関係

  KATSUDA Tomohisa, MORIMOTO Takaaki, YAMAJI Hideki

  化学工学会第83年会, Mar. 2018, 化学工学会, 吹田


• 昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産

  MATSUDA Takuya, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第83年会, Mar. 2018, 化学工学会, 吹田


• 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産

  MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第49回秋季大会, Sep. 2017, 化学工学会, 名古屋


• 共焦点レーザー走査顕微鏡観察と数値ミュレーションに基づく抗体の吸着・破過の解析

  HIROOKA Kanamu, YAMAGUCHI Tomoki, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第49回秋季大会, Sep. 2017, 化学工学会, 名古屋


• エアリフト式フォトバイオリアクターの操作条件と微細藻類の光独立栄養増殖の関係

  KUREBAYASHI Aika, MORIMOTO Takaaki, NISHIMURA Takumi, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第49回秋季大会, Sep. 2017, 化学工学会, 名古屋


• Production of influenza virus proteins by recombinant insect cells

  YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa

  International Meeting & 42nd Annual Conference of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Aug. 2017, The Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Kuala Lumpur, Malaysia


• 昆虫細胞を用いたインフルエンザウイルスタンパク質の生産

  YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa

  第30回日本動物細胞工学会2017年度大会 (JAACT2017), Jul. 2017, 日本動物細胞工学会, 松山


• Efficient protein production by transient gene expression using insect cells

  YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa

  25th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2017), May 2017, European Society for Animal Cell Technology (ESACT), Lausanne, Switzerland


• Transient gene expression in insect cells for efficient recombinant antibody production

  TAKAHASHI Ryoma, MASUMI-KOIZUMI Kyoto, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki

  The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, 日本動物細胞工学会, 神戸


• Production of virus-like particles using recombinant insect cells

  TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki

  The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, 日本動物細胞工学会, 神戸


• Characterization of the diffusion and adsorption of IgG in protein A-coupled adsorbents with confocal laser scanning microscopy

  HIROOKA Kanamu, HARUNA Yusuke, YAMAJI Hideki, KATSUDA Tomohisa

  The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, 日本動物細胞工学会, 神戸


• Efficient antibody production by transient gene expression in insect cells

  YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa

  17th European Congress on Biotechnology (ECB2016), Jul. 2016, European Federation of Biotechnology (EFB), Krakow, Poland


• 培養条件の概日変化が緑藻Haematococcus pluvialisの栄養増殖に及ぼす影響

  Kohei YOSHIMINE, YAMADA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第81年会, Mar. 2016, 化学工学会, 大阪


• 共焦点レーザー走査顕微鏡観察に基づくProtein A固定化吸着剤の拡散・吸着特性の評価

  KATSUDA Tomohisa, HARUNA Yusuke, HIROOKA Kanamu, KASE Yuki, OTA Kou, YOSHIKAWA Toru, YAMAJI Hideki

  化学工学会第81年会, Mar. 2016, 化学工学会, 大阪


• Recombinant antibody production by transient gene expression in insect cells

  YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa

  Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia


• Photoautotrophic growth of Haematococcus pluvialis under controlled temperature and light conditions

  KATSUDA Tomohisa, GIANNELLI Luca, YAMADA Hiroyuki, YAMAJI Hideki

  Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia


• Optimization of microalgal cultivation in cascade photobioreactors with wavy bottom via computational fluid dynamics

  WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa

  Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia


• シトクロムP450発現大腸菌を用いたwhole cell bioconversionに及ぼす培養・反応条件の影響

  HARADA Masayuki, MORINAKA Ryohei, KATSUDA Tomohisa, IMAISHI Hiromaisa, YAMAJI Hideki

  化学工学会第47回秋季大会, Sep. 2015, 化学工学会, 札幌


• 昆虫細胞を宿主とした一過性発現による抗体タンパク質の生産

  MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki

  第28回日本動物細胞工学会2015年度大会 (JAACT2015), Jul. 2015, 日本動物細胞工学会, 仙台


• Production of an antibody molecule by transient gene expression in insect cells

  MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki

  24th ESACT Meeting Barcelona 2015, Jun. 2015, European Society for Animal Cell Technology (ESACT), Barcelona, Spain


• 鉛に汚染された土壌の評価とバイオレメディエーションによる鉛の回収

  塩見 尚史, SUETSUGU KENICHIRO, KATSUDA TOMOHISA

  日本ＬＣＡ学会, Mar. 2015


• カスケード型フォトバイオリアクターにおける微細藻類培養の数値流体力学に基づく検討

  WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会姫路大会, Dec. 2014, 化学工学会関西支部, 姫路


• Transient gene expression in insect cells for recombinant antibody production

  MORI Keita, HAMADA Hirotsugu, OGAWA Takahumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki

  The 27th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT2014 Kitakyushu), Nov. 2014, 日本動物細胞工学会, 北九州


• 昆虫細胞を宿主とした一過性発現による抗体タンパク質生産

  HAMADA Hirotsugu, MORI Keita, OGAWA Takafumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第46回秋季大会, Sep. 2014, 化学工学会, 福岡


• Production of antibody fragments by transient gene expression in insect cells

  YAMAJI Hideki, KATSUDA Tomohisa, HAMADA Hirotsugu, MORI Keita

  16th European Congress on Biotechnology (ECB16), Jul. 2014, European Federation of Biotechnology, Edinburgh, UK


• 昆虫細胞を用いた一過性発現による機能性抗体タンパク質の生産

  MORI Keita, HAMAMOTO Kohei, MIYAGUCHI Naoko, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第45回秋季大会, Sep. 2013, 化学工学会, 岡山市


• Haematococcus pluvialisの増殖とアスタキサンチン生産に及ぼす培養温度の影響

  YAMADA Hiroyuki, WADA Sotaro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第45回秋季大会, Sep. 2013, 化学工学会, 岡山市


• Photobioreactor design using CFD: mixing and light effects on microalgal cultures

  GIANNELLI Luca, WADA Sotaro, YAMADA Hiroyuki, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第78年会, Mar. 2013, 化学工学会, 豊中市


• Production of recombinant antibody molecules in insect cells

  MIYAGUCHI Naoko, HAMAMOTO Kouhei, OGAWA Takafumi, SONODA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki

  The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, 日本動物細胞工学会, 名古屋市


• Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells

  HOTTA Yuri, MINAMITANI Azusa, NARITA Kazuma, KATSUDA Tomohisa, YAMAJI Hideki

  The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, 日本動物細胞工学会, 名古屋市


• 組換え昆虫細胞によるscFv-Fc融合タンパク質の生産

  SONODA Hiroyuki, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki

  第64回日本生物工学会大会, Oct. 2012, 日本生物工学会, 神戸市


• Computational fluid dynamics for enhancing the light distribution in a photobioreactor grown culture of Hematococcus pluvialis

  GIANNELLI Luca, WADA Sotaro, YAMAJI Hideki, KATSUDA Tomohisa

  第64回日本生物工学会大会, Oct. 2012, 日本生物工学会, 神戸市


• 大腸菌を用いた一本鎖抗体生産における終止コドンの影響

  YAMAJO Yuki, FUKUMARU Yuka, YAMAJI Hideki, KATSUDA Tomohisa

  化学工学会第44回秋季大会, Sep. 2012, 化学工学会, 仙台市


• 気液界面を利用した高収率リポソーム生成法の開発

  Hirohiko AIHARA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Ruri HIDEMA

  化学工学会第44回秋季大会仙台, Sep. 2012, 仙台


• 昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産

  YAMAJI Hideki, SEGAWA Maiko, NAKAMURA Masataka, KATSUDA Tomohisa

  化学工学会第77年会, Mar. 2012, 化学工学会, 東京


• 炭化水素資化酵母Yarrowia lipolyticaによる油汚染土壌の修復のための無機栄養源の開発

  FUJIMOTO Sho, TANUMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第43回秋季大会, Sep. 2011, 化学工学会, 名古屋


• 昆虫細胞を用いた抗体タンパク質の分泌生産

  HAMAMOTO Kouhei, MIYAGUCHI Naoko, SAWAMOTO Shiori, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第43回秋季大会, Sep. 2011, 化学工学会, 名古屋


• 昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討

  NARITA Kazuma, HOTTA Yuri, MINAMITANI Azusa, NAGASUGA Takashi, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第43回秋季大会, Sep. 2011, 化学工学会, 名古屋


• シャイン・ダルガーノ配列を改変した大腸菌による一本鎖抗体の流加培養生産

  FUKUMARU Yuka, NOGAMI Tatsuhiro, KIHARA Mana, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第43回秋季大会, Sep. 2011, 化学工学会, 名古屋


• Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells

  YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

  IUMS 2011 Sapporo Congress, Sep. 2011, International Union of Microbiological Societies, 札幌


• Secretory production of virus-like particles by recombinant insect cells

  YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

  ESACT 2011 Meeting, May 2011, European Society for Animal Cell Technology, Vienna, Austria


• 炭化水素資化酵母Yarrowia lipolyticaを用いた油汚染土壌の修復における栄養源の添加条件の検討

  FUJIMOTO Shou, TANUMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki

  第13回化学工学会学生発表会(神戸大会), Mar. 2011, 化学工学会, 神戸市


• 大腸菌を用いた一本鎖抗体生産におけるシャイン・ダルガーノ配列の影響

  FUKUMARU Yuka, NOGAMI Tatsuhiro, KATSUDA Tomohisa, YAMAJI Hideki

  第13回化学工学会学生発表会(神戸大会), Mar. 2011, 化学工学会, 神戸市


• 大腸菌におけるインクルージョンボディ生成に及ぼすシャイン・ダルガーノ配列の影響

  KATSUDA Tomohisa, FUKUMARU Yuka, KIHARA Mana, NOGAMI Tatsuhiro, YAMAJI Hideki

  化学工学会第76年会, Mar. 2011, 化学工学会, 東京都


• リポソームの応用事例

  KATSUDA Tomohisa

  近畿バイオインダストリー振興会議第5回フォローアップ勉強会, Jan. 2011, 近畿バイオインダストリー振興会議, 大阪市


• Optimized Shine-Dalgarno sequence for efficient production of single-chain Fv antibody in Escherichia coli

  NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), Dec. 2010, Canadian Society for Chemistry, American Chemical Society, Chemical Society of Japan, New Zealand Institute of Chemistry, Royal Australian Chemical Institute, Korean Chemical Society, Chinese Chemical Society, Honolulu, USA


• Rapid production of functional antibody molecules by transient gene expression in insect cells

  SAWAMOTO Shiori, HAMAMOTO Kouhei, KATSUDA Tomohisa, YAMAJI Hideki

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan


• Effect of signal peptide on the production of single-chain fv antibody by Escherichia coli

  NOGAMI Tatsuhiro, FUKUMARU Yuka, KATSUDA Tomohisa, YAMAJI Hideki

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan


• Production of Japanese encephalitis virus-like particles in insect cell expression systems

  YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

  The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010), Oct. 2010, Asia Pacific Confederation of Chemical Engineering, Taipei, Taiwan


• マイクロ流路内におけるリポソーム生成時のマイクロ流体挙動に関する研究

  FUJIWARA Jun, SUZUKI Hiroshi, KOMODA Hiroyuki, KATSUDA Tomohisa

  化学工学会第42回秋季大会, Sep. 2010, 化学工学会, 京都市


• プロテインAアフィニティクロマトグラフィーのための溶離条件検討法の開発

  KASE Yuki, NISHIYAMA Takuya, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第42回秋季大会, Sep. 2010, 化学工学会, 京都市


• シャペロン共発現による大腸菌での一本鎖抗体 (scFv) 融合タンパク質の生産

  SONODA Hiroyuki, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第42回秋季大会, Sep. 2010, 化学工学会, 京都市


• アルカン資化酵母Yarrowia lipolyticaを用いた土壌汚染油の分解

  TANUMA Naoki, FUJIMOTO Shou, SHIOMI Naofumi, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第42回秋季大会, Sep. 2010, 化学工学会, 京都市


• Production of Japanese encephalitis virus-like particles using insect cells

  NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, YAMAJI Hideki

  The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010), Sep. 2010, The Japanese Association for Animal Cell Technology, 札幌市


• Pichia pastorisを用いた一本鎖抗体生産における誘導初期のメタノール濃度の影響

  FUJITA Keisuke, AKIYAMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第75年会, Mar. 2010, 化学工学会, 鹿児島


• 昆虫細胞-バキュロウイルス系を用いた抗体タンパク質の高効率生産

  SAWAMOTO Shiori, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki

  第8回最先端バイオテクノロジー発表会, Feb. 2010, 化学工学会, 大阪


• 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響

  NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  第8回最先端バイオテクノロジー発表会, Feb. 2010, 化学工学会, 大阪


• Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli

  KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2009, Dec. 2009, Young Asian Biochemical Engineers Community, Xiamen


• Production of Japanese encephalitis virus-like particles by recombinant insect cells

  YAMAJI Hideki, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

  Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC '09), Nov. 2009, APBioChEC '09 Committee, 神戸市


• Oil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolytica

  FUKUI Mayu, SHIOMI Shinji, TANUMA Naoki, KATSUDA Tomohisa, SHIOMI Naofumi, YAMAJI Hideki

  The 9th Asia-Pacific Biochemical Engineering Conference 2009, Nov. 2009, The 9th Asia-Pacific Biochemical Engineering Conference 2009, Kobe


• 大腸菌を用いた一本鎖抗体生産におけるSD配列の影響

  NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第41回秋季大会, Sep. 2009, 化学工学会, 東広島市


• 昆虫細胞を用いた日本脳炎ウイルス様粒子の生産

  TAKAHASHI Yusuke, NAGASUGA Takashi, NAKAMURA Masataka, KATSUDA Tomohisa, YAMAJI Hideki

  第61回日本生物工学会大会, Sep. 2009, 日本生物工学会, 名古屋市


• 昆虫細胞-バキュロウイルス系による抗体タンパク質の生産

  SAWAMOTO Shiori, SUZUKI Tasuku, FURUTA Takanori, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第41回秋季大会, Sep. 2009, 化学工学会, 東広島市


• ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用

  IWASAKI Daiki, SENGA Itaru, YAMASHITA Takashi, TONAI Masahiro, OGINO Chiaki, SHIMIZU Nobuaki, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第41回秋季大会, Sep. 2009, 化学工学会, 東広島市


• 昆虫細胞を用いた日本脳炎ウイルスタンパク質の生産

  TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第74年会, Mar. 2009, 化学工学会, 横浜市


• 一塩基変異を検出する昇温溶出アフィニティクロマトグラフィーに対するPCRの適用性

  KATSUDA Tomohisa, IMANISHI Makoto, KATOH Shigeo, YAMAJI Hideki

  化学工学会第74年会, Mar. 2009, 化学工学会, 横浜市


• マイクロ流路内におけるリポソーム生成機構

  FUJIWARA Jun, HAMAMURA Junya, SUZUKI Hiroshi, KATSUDA Tomohisa, KOMODA Hiroyuki, USUI Hiromoto

  化学工学会第74年会, Mar. 2009, 化学工学会, 横浜


• Production of adenovirus vector by 293 cells immobilized within porous biomass support particles

  MORISHITA Naoya, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki

  The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, 日本動物細胞工学会, 福岡市


• Low multiplicity infection of 293 cells for adenovirus vector production

  MORISHITA Naoya, YAMADA Kentaro, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki

  The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, 日本動物細胞工学会, 福岡市


• High efficiency expression of soluble scFv in E. coli by use of linkers containing rare codons

  KAJIHARA Hideyuki, SAKAN Yoshinobu, KIHARA Mana, KIKUCHI Yasufumi, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki, KATOH Shigeo

  The 14th Symposium of Young Asian Biochemical Engineers’ Community, Nov. 2008, Young Asian Biochemical Engineers’ Community, 東京都


• Effects of the parameters of flashing light from blue emitting diodes on astaxanthin production by H. pluvialis

  SENGA Itaru, SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2008, Nov. 2008, Young Asian Biochemical Engineers Community, Tokyo


• A biochemical engineering study of photo-bioproces

  KATSUDA Tomohisa

  The 2 nd SCEJ (Kansai-Branch)/SSCCI Joint International Conference on Chemical Engineering, Nov. 2008, SCEJ (Kansai-Branch) and SSCCI, Shanghai, China


• Secretory production of Japanese encephalitis virus E protein by recombinant insect cells

  TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki

  “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, Hanyang University, Ansan, Korea


• Detection of single nucleotide variation in amplified gene fragments using oligonucleotide-coupled affinity column

  KATSUDA Tomohisa, IMANISHI Makoto, YAMAJI Hideki, KATOH Shigeo

  “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, Hanyang University, Ansan, Korea


• 多孔性粒子に固定化した大腸菌を用いる有用物質生産

  JIN Na, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki

  化学工学会第40回秋季大会, Sep. 2008, 化学工学会, 仙台市


• レアコドンリンカーによる大腸菌での可溶性scFvの高効率生産

  KIHARA Mana, SAKAN Yoshinobu, KAJIHARA Hideyuki, AKASAKA Sho, KATSUDA Tomohisa, KATOH Shigeo, YAMAJI Hideki

  化学工学会第40回秋季大会, Sep. 2008, 化学工学会, 仙台市


• 微生物の光合成を利用した有用物質生産ーエネルギーと食品への活用ー

  勝田 知尚

  第108回微小光学研究会, Jul. 2008


• Liposome Formation Characteristics with a Micro-Reactor

  Jun-ya HAMAMURA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Shigeo KATO, Hiromoto USUI

  11th Liposome Research Days Conference, Jul. 2008, Yokohama, Japan


• Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen species

  KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Daiki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  American Society for Microbiology 108th General Meeting, Jun. 2008, American Society for Microbiology, Boston, USA


• マイクロ流路を用いたリポソームの生成機構

  鈴木 洋, 浜村 隼矢, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基

  化学工学会 第73年会, Mar. 2008, 静岡


• Enhancement of growth and astaxanthin production of Haematococcus pluvialis by using airlift photobioreactor

  Ranjbar Reza, Inoue Ryota, Iwasaki Daiki, Katsuda Tomohisa, Katoh Shigeo

  化学工学会第73年会, Mar. 2008, 化学工学会, 浜松


• ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化

  SENGA Itaru, YAMAGAMI Keisuke, KATSUDA Tomohisa, OGINO Chiaki, SHIMIZU Nobuaki, KATOH Shigeo

  第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, 化学工学会関西支部、化学工学会バイオ部会, 西宮


• シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価

  TAKEDA Naoki, KATSUDA Tomohisa, KATOH Shigeo, MIYAHARA Hiroyoshi, INOUE Masaki, NAKAMURA Shuji

  第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, 化学工学会関西支部、化学工学会バイオ部会, 西宮


• High efficiency production of astaxantin by autotrophic cultivation of Haematococcua pluvialis in column photobioreactors

  RANJBAR Reza, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo

  第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, 化学工学会関西支部、化学工学会バイオ部会, 西宮


• Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination

  SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo

  上海市化学化工学会・化学工学会関西支部 第1回若手研究交流会, Dec. 2007, 上海市化学化工学会、化学工学会関西支部, 大阪


• 青色点滅光照射下でのH. pluvialisの培養に及ぼす光強度および周波数の影響

  SHIRAISHI Hironori, ISHIZU Naoki, SENGA Itaru, RANJBAR Reza, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会山口大会, Nov. 2007, 化学工学会中国四国支部, 宇部


• Application of PCR to Affinity Chromatography for SNP Detection

  KATSUDA Tomohisa, KATOH Shigeo

  The 6th Asia-Europe Biorecognition Engineering Society Conference, Nov. 2007, Asia-Europe Biorecognition Engineering Society, Taoyuan, Taiwan


• Oxidative treatment of H. pluvialis with the sonocatalytic reaction

  KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Hiroki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, Young Asian Biochemical Engineers Community, Seoul, Korea


• Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination

  SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, Young Asian Biochemical Engineers Community, Seoul, Korea


• A Biochemical Engineering Study of Bioproductions using Photobioreactor

  KATSUDA Tomohisa

  2007 Fall KSBB Meeting & International Symposium, Oct. 2007, The Korean Society for Biotechnology and Bioengineering, Daegu, Korea


• 二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導

  KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  第59回生物工学会大会, Sep. 2007, 日本生物工学会, 広島


• 気泡塔型フォトバイオリアクターを用いたヘマトコッカス藻による効率的アスタキサンチン生産

  INOUE Ryota, Ranjbar Reza, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第39回秋季大会, Sep. 2007, 化学工学会, 札幌


• マイクロキャピラリー内におけるリポソーム生成特性に関する研究

  濱村 隼矢, 鈴木 洋, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基

  化学工学会 第39回秋季大会, Sep. 2007, 北海道大学


• フォトバイオリアクターによる 有用物質生産のための生物化学工学的検討

  KATSUDA Tomohisa

  第59回生物工学会大会, Sep. 2007, 日本生物工学会, 広島


• Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasounds

  KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  13th European Congress on Biotechnology, Sep. 2007, European Federation of Biotechnology, Barcelona, Spain


• DNAアフィニティカラムを用いるSNP検出法へのPCR増幅法の応用

  IMANISHI Makoto, NISHIWADA Yasushi, FUKUI Mayu, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第39回秋季大会, Sep. 2007, 化学工学会, 札幌


• 青色点滅光照射下のH. pluvialisにおけるアスタキサンチン生産量と光強度との関係

  SHIRAISHI Hironori, YAMAGAMI Keisuke, RANJBAR Reza, SENGA Itaru, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第72年会, Mar. 2007, 化学工学会, 京都


• メタノール資化性酵母を用いた一本鎖抗体生産におけるメタノール供給方法

  OHNISHI Yuka, YAMAWAKI Shinya, MATSUMOTO Takehiro, SHIOMI Naofumi, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第72年会, Mar. 2007, 化学工学会, 京都


• シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価

  TAKEDA Naoki, IMADA Masami, KATSUDA Tomohisa, KATOH Shigeo, MIYAHARA Hiroyoshi, INOUE Masaki, NAKAMURA Shuji

  化学工学会第72年会, Mar. 2007, 化学工学会, 京都


• Optimization of silica based media for antibody purification by protein A affinity chromatography

  IMADA Masami, TAKEDA Naoki, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji, KATOH Shigeo

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, Young Asian Biochemical Engineers Community, Kaohsiung


• Effects of flashing light from blue LEDs on the cell growth and astaxanthin production of Haematococcus pluvialis

  YAMAGAMI Keisuke, SHIRAISHI Hironori, RANJBARDAVIJANI Reza, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, Young Asian Biochemical Engineers Community, Kaohsiung


• Optimization of silica-based matrices for antibody purification by protein A affinity chromatography

  KATOH Shigeo, IMADA Masami, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji

  26th international symposium on the separation of proteins, peptides and polynucleotides, Oct. 2006, ISPPP, Innsbruck


• 昇温溶出アフィニティークロマトグラフィーによる一塩基多型検出法の開発

  KATSUDA Tomohisa

  化学工学会バイオ部会員フォーマルセミナー, Sep. 2006, 化学工学会バイオ部会, 福岡


• メタノール資化性酵母Pichia pastorisの連続培養による一本鎖抗体生産

  YAMAWAKI Shinya, MATSUMOTO takehiro, OHNISHI Yuka, KATSUDA Tomohisa, SHIOMI Naofumi, KATOH Shigeo

  第58回生物工学会大会, Sep. 2006, 生物工学会, 大阪


• ヘマトコッカスによるアスタキサンチン生産における青色点滅光照射の影響

  KATSUDA Tomohisa, SHIRAISHI Hironori, YAMAGAMI Keisuke, SENGA Itaru, SHIMAHARA Kazumichi, KATOH Shigeo

  第58回生物工学会大会, Sep. 2006, 生物工学会, 大阪


• ヘマトコッカスによるアスタキサンチン生産における青色パルス光照射条件の検討

  SHIRAISHI Hironori, YAMAGAMI Keisuke, RANJBAR Reza, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第38回秋季大会, Sep. 2006, 化学工学会, 福岡


• Pseudomonas sp. を用いる自己固定化バイオレメディエーションによるシアヌル酸分解

  SHIOMI Naofumi, ARII Kenji, NAKAI Hiroaki, YAMAGUCHI Yutaka, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第38回秋季大会, Sep. 2006, 化学工学会, 福岡


• Photocultivation of Haematococcus pluvialis Using Multi-Compartment Photobioreactor

  YOSHIMURA Satoshi, INOUE Ryota, RANJBER Reza, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第71年会, Mar. 2006, 化学工学会, 東京


• SNP Detection Method Using Temperature Gradient Affinity Chromatography

  NISHIWADA Yasushi, KAMURA Mitsumasa, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第71年会, Mar. 2006, 化学工学会, 東京


• Flashing light Illumination for Effective Production of Astaxanthin by Haematococcus pluvialis

  SHIMAHARA Kazumichi, KATSUDA Tomohisa, YAMAGAMI Keisuke, SHIRAISHI Hironori, KATOH Shigeo

  化学工学会第71年会, Mar. 2006, 化学工学会, 東京


• Effects of Iron Ion Concentration on the Growth Stability of Photosynthetic Bacteria in the Semi-Continuous Cultivation

  KATSUDA Tomohisa, YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, INOUE Ryota, KATOH Shigeo

  関西地区3学協会合同大会, Nov. 2005, 近畿化学協会, 化学工学会, 触媒学会, 大阪


• Bioremediation of Acidified Pond and Soil

  SHIOMI Naofumi, NABESHIMA Risako, YASUDA Takako, IWASAKI Saori, YAMAGUCHI Yutaka, NAKAI Hiroaki, KATSUDA Tomohisa, KATOH Shigeo

  関西地区3学協会合同大会, Nov. 2005, 近畿化学協会, 化学工学会, 触媒学会, 大阪


• Effects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatus

  YOSHIMURA Satoshi, YEGANI Reza, MORIYA Kazunori, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo

  関西地区3学協会合同大会, Nov. 2005, 近畿化学協会, 化学工学会, 触媒学会, 大阪


• Detection of Single Nucleotide Polymorphysms by Affinity Chromatography with DNA Coupled Column

  NISHIWADA Yasushi, KAMURA Mitsumasa, KATSUDA Tomohisa, KATOH Shigeo

  関西地区3学協会合同大会, Nov. 2005, 近畿化学協会, 化学工学会, 触媒学会, 大阪


• Bioremediation of Herbicide-Polluted Soil with Self-Immobilized Microorganisms

  NAKAI Hiroaki, YAMAGUCHI Yutaka, YASUDA Takako, ARII Kenji, SAKAI Toyohide, KATSUDA Tomohisa, KATOH Shigeo, SHIOMI Naofumi

  平成17年度日本生物工学会大会, Nov. 2005, 日本生物工学会, 筑波


• Rapid SNP Detection by Temperature Gradient Affinity Chromatography

  KATOH Shigeo, KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi

  25th International Symposium on the Separation of Proteins, Peptides & Polynucleotides, Nov. 2005, International Symposium on the Separation of Proteins, Peptides & Polynucleotides, St. Pete Beach, Florida


• Effective Production of Astaxanthin from Haematococcus pluvialis by Use of Light Emitting Diodes

  SHIMAHARA Kazumichi, YAMAGAMI Keisuke, SHIRAISHI Hironori, KATSUDA Tomohisa, KATOH Shigeo

  関西地区3学協会合同大会, Nov. 2005, 近畿化学協会, 化学工学会, 触媒学会, 大阪


• Detection of SNP Using Affinity Chromatography of Immobilized Oligonucleotide

  KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo

  Young Asian Biochemical Engineers Community (YABEC) Symposium 2005, Oct. 2005, Young Asia Biochemical Engineers Community, Beijing, China


• Effects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatus

  KATSUDA Tomohisa, YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, INOUE Ryota, KATOH Shigeo

  化学工学会第37回秋季大会, Sep. 2005, 化学工学会, 岡山


• Growth Sstability of Photosynthetic Bacteria : Effects of Supplemental Illumination and Ferrous Ion Concentration

  YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo

  The 12th European Congress on Biotechnology, Aug. 2005, European Federation of Biotechnology, Copenhagen, Denmark


• Fed-Batch Cultivation of Haematococcus pluvialis under Illumination with LEDs for Production of Astaxanthin

  LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo

  The 12th European Congress on Biotechnology, Aug. 2005, European Federation of Biotechnology, Copenhagen, Denmark


• Temperature Gradient Elution of DNAs from an Oligo-DNA Immobilized Column

  KATSUDA Tomohisa, KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATOH Shigeo

  分離技術会年会2005, Jun. 2005, 分離技術会, 大阪


• Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis

  KATSUDA Tomohisa, LABABPOUR Abdolmajid, SHIMAHARA Kazumichi, KATOH Shigeo

  Asia-Pacific Biochemical Engineering Conference 2005, May 2005, The Korean Society for Biotechnology and Bioengineering, The Korean Institute of Chemical Engineers, Jeju, Korea


• Detection of SNP using Affinity Chromatography of Immobilized Oligonucleotide

  KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo

  The 4th Asia-Europe Biorecognition Engineering Society Conference, May 2005, Asia-Europe Biorecognition Engineering Society, Ansan, Korea


• Effects of Nutrients Feeding Method on Astaxanthin Production by Haematococcus pluvialis

  KATSUDA Tomohisa, LABABPOUR Abdolmajid, KYOUI Yoshiaki, SHIMAHARA Kazumichi, KATOH Shigeo

  化学工学会第70年会, Mar. 2005, 化学工学会, 名古屋


• Elution Behavior of DNA from Oligo-DNA Immobilized Column under Temperature Gradient

  KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATSUDA Tomohisa, KATOH Shigeo

  化学工学会第70年会, Mar. 2005, 化学工学会, 名古屋


• Astaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps

  LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo

  2004 AIChE Annual Meeting, Nov. 2004, American Institute of Chemical Engineers, Austin, TX


• Development of Oligonucleotide Immobilized Capillary Chromatography

  KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, HOSSAIN Zakir, KATOH Shigeo

  The 2nd International Conference on Bioseparation Engineering, Oct. 2004


• フォトバイオリアクターにおける光強度分布と増殖速度安定性の工学的検討

  イエガニ レザ, 吉村 誠司, 森谷 一徳, 勝田 知尚, 加藤 滋雄

  日本生物工学会平成16年度大会, Sep. 2004, 日本生物工学会, 名城大学


• オリゴDNA固定化担体を用いた一塩基多型（SNP）の検出法の検討

  西島 研, 嘉村 光真, 勝田 知尚, 加藤 滋雄

  日本生物工学会平成16年度大会, Sep. 2004, 日本生物工学会, 名城大学


• Instability of Growth Rate in Dense Culture of Photosynthetic Cells

  YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, KATSUDA Tomohisa, KATOH Shigeo

  Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, Young Asian Biochemical Engineers' Community (YABEC), Osaka


• Induction of Astaxanthin Accumulation in H. pluvialis by IIIumination with LED Lamps and Fed Batch Addition of Nutrients

  LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo

  Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, Young Asian Biochemical Engineers' Community (YABEC), Osaka


• Growth and Astaxanthin Accumulation in H. pluvialis Cultures Illuminated by LED Lamps

  Lababpour Abdolmajid, 羽田 圭司, 島原 一道, 勝田 知尚, 加藤 滋雄

  化学工学会第69年会, Apr. 2004, 化学工学会, 大阪府立大学


• 緑藻Haematococcus pluvialisによるアスタキサンチン生産におよぼす青色パルス光の影響

  嶋原 一道, LABABPOUR Abdolmajid, 羽田 圭志, 勝田 知尚, 加藤 滋雄

  第55回日本生物工学会大会, Sep. 2003, 日本生物工学会, 熊本大学


• 光合成微生物の半連続培養における増殖安定性におよぼす光強度分布の影響

  勝田 知尚, イエガニ レザ, 吉村 誠司, 藤井 信彰, 五十嵐 啓介, 加藤 滋雄

  第55回日本生物工学会大会, Sep. 2003, 日本生物工学会, 熊本大学


• パルス光照射下におけるヘマトコッカスによるアスタキサンチン生産

  勝田 知尚, LABABPOUR Abdolmajid, 嶋原 一道, 羽田 圭志, 加藤 滋雄

  化学工学会 第36回秋季大会, Sep. 2003, 化学工学会, 東北大学


• The effects of light intensity distribution on the stability of growth rate of a photosynthetic microorganism Rhodobacter capsulatus

  YEGANI Reza, 藤井 信彰, 五十嵐 啓介, 吉村 誠司, 勝田 知尚, 加藤 滋雄

  化学工学会 第36回秋季大会, Sep. 2003, 化学工学会, 東北大学


• Cultivation of microalga in photobioreactors illuminated with LEDs

  KATOH Shigeo, LABABPOUR Abdolmajid, KATSUDA Tomohisa

  The 6th Italian Conference on Chemical and Process Engineering, Jun. 2003, 未記入, 未記入


• Development of a new SNP detectable method by use of the immobilized oligonucleotide capillary chromatography

  Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Shigeo Katoh

  Asia-Europe Symposium on Biomolecular Recognition, May 2003


• 緑藻Haematococcus pluvialisによるアスタキサンチン生産における光照射法の検討

  勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄

  化学工学会第68年会, Mar. 2003


• 二槽式バイオリアクターによる光合成微生物の生産性の向上

  藤井 信彰, 五十嵐 啓介, Reza Yegani, 勝田 知尚, 加藤 滋雄

  化学工学会第68年会, Mar. 2003


• Astaxanthin production by Haematococcus pluvialis using LED lamps

  Tomohisa Katsuda, Abdolmajid Lababpour, Kazumichi Shimahara, Shigeo Katoh

  Young Asian Biochemical Engineers' Community (YABEC) Symposium 2002, Nov. 2002


• 緑藻Haematococcus pluvialisによるアスタキサンチン生産に及ぼす照射光の影響

  Abdolmajid Lababpour, 嶋原 一道, 勝田 知尚, 加藤 滋雄

  化学工学会第35回秋季大会, Sep. 2002


• 発光ダイオードを用いた緑藻Haematococcus pluvialisによるアスタキサンチン生産

  勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄

  化学工学会第35回秋季大会, Sep. 2002


• タンパク質のリフォールディングにおよぼす変性剤除去の影響

  宇高 太郎, 神野 一也, M.D. Farshbaf, 勝田 知尚, 加藤 滋雄

  化学工学会第35回秋季大会, Sep. 2002


• 高効率土壌汚染浄化法を利用した酸性雨の中和

  安田 多賀子, 岩崎 沙織, 勝田 知尚, 塩見 尚史, 加藤 滋雄

  化学工学会鳥取大会, Jul. 2002


• 光合成微生物培養液中における光強度減衰モデルの適用性

  勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄

  化学工学会第67年会, Mar. 2002


• B. subtilisの自己固定化能力を利用した酸性雨の土壌中での中和

  安田 多賀子, 岩崎 沙織, 井上 洋子, 楠本 智子, 勝田 知尚, 塩見 尚史, 加藤 滋雄

  化学工学会第67年会, Mar. 2002


• Light attenuation in suspension of purple bacterium Rhodobacter capsulatus and green alga Chlamydomonas reinhardtii

  Tomohisa Katsuda, Nobuaki Fujii, Natsushi Takata, Shigeo Katoh

  Young Asian Biochemical Engineers' Community (YABEC) Symposium 2001, Oct. 2001


• 光合成微生物培養液中における光強度減衰

  勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄

  日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 2001


• 光合成微生物培養液中での光強度減衰に及ぼす光源の影響

  勝田 知尚, 藤井 信彰, 加藤 滋雄

  化学工学会福井大会, Jul. 2001


• 光合成微生物培養液中での光強度減衰

  勝田 知尚, 藤井 信彰, 大嶋 寛, 加藤 滋雄

  化学工学会第66年会, Apr. 2001


• テーラー渦型反応器によるタンパク質の連続リフォールディング

  加藤 芳寛, M.D. Farshbaf, 宇高 太郎, 野田 秀夫, 勝田 知尚, 加藤 滋雄

  化学工学会第66年会, Apr. 2001


• 円筒型光リアクターの新規な光強度分布モデルと光合成細菌による水素生産への適用

  勝田 知尚, 有本 武司, 五十嵐 幸一, 東 雅之, 大嶋 寛

  化学工学会第32回秋季大会, Sep. 1999


• 円筒型フォトバイオリアクターの新規な光強度分布モデルと光合成微生物による水素生産への適用

  勝田 知尚, 大嶋 寛

  日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1999


• エタノールアミンを窒素源として用いた光合成細菌Rhodobacter capsulatusによる水素生産

  勝田 知尚, 大嶋 寛

  日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1998


• エタノールアミンを窒素源として用いた、Rhodobacter capsulatusによる水素生産

  勝田 知尚, 大嶋 寛, 東 雅之, 加藤 錠治, 高桑 進

  平成9年度日本生物工学会大会, Sep. 1997


• Hydrogen detectable color indicator for screening of microorganisms

  Tomohisa Katsuda, Hiroshi Ooshima, Masayuki Azuma, Jyoji Kato

  化学工学会第29回秋季大会, Sep. 1996

• European Federation of Biotechnology

  May 2007 - Present


• Asian Federation of Biotechnology


• 日本生物工学会


• 化学工学会

• 培養温度制御に基づく真核細胞への遺伝子導入の効率化

  勝田 知尚

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2021 - 31 Mar. 2024

  本研究では，研究代表者らが緑藻の培養で見出した，培養温度を適切な範囲で周期的に変化させると培養液中の細胞間で細胞周期が同調するという知見に基づき，真核細胞の細胞周期を同調させることにより，核膜の消失から再構成の間にあわせて外来遺伝子を導入できるようにし，これによって外来遺伝子の核内移行，ならびに核DNAとの接触を促進させて，真核細胞への遺伝子導入の効率化を図ることを検討する． 令和3年度には，開放型の有糸分裂を行う昆虫細胞に注目し，培養温度をプログラム制御できるインキュベーターを用いて培養を行い，細胞周期が同調する温度制御条件の検討を行った．昆虫細胞には，組換えタンパク質の発現に広く利用されており，子宮頸がんワクチンの生産にも利用されているTrichoplusia ni BTI-TN-5B1-4 (High Five)細胞を用いた． はじめに，培養温度を最適よりも高温側で一定に保持して増殖挙動を調べた．High Five細胞は35℃までは最適温度である27℃のときと同等の増殖速度ならびに最高到達細胞密度を示した．しかし，37℃では増殖が顕著に抑制され，39℃では増殖しないことが分かった．培養液中のそれぞれの細胞の細胞周期をフローサイトメーターにより分析したところ，G2/M期にある細胞の全細胞数に対する割合が27℃の15－20%から37℃の30－40%まで，温度上昇に伴って増加することを見出した． そこで，培養温度は27℃と37℃を12 h周期で変化させることとし，High Five細胞の細胞周期の同調を試みた．しかし，植継ぎを行いながら6 dにわたって培養を継続したが，細胞周期が同調したときに見られる細胞密度の倍加は観察されなかった．High Five細胞では緑藻と同様にして細胞周期を同調させることは困難であることが分かった．


• 微細藻の増殖特性向上に向けた熱ショック応答の応用

  勝田 知尚

  学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020


• Production of influenza virus-like particles in recombinant insect cells

  Yamaji Hideki, KATSUDA Tomohisa

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2015 - 31 Mar. 2019

  Production of influenza virus-like particles in recombinant insect cells was investigated. The cDNA fragments encoding hemagglutinin (HA) and matrix protein 1 (M1) of an influenza virus A were separately cloned into the plasmid vector pIHAbla and pIHAneo. The pIHAbla and pIHAneo contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with either a blasticidin or neomycin resistance gene for use as a selectable marker, respectively. After cotransfection with the prepared plasmids, High Five cells were incubated with blasticidin and G418, and cells resistant to the antibiotics were effectively obtained. Sucrose density-gradient sedimentation analysis of the culture supernatant showed that secreted HA and M1 molecules were produced in a particulate form.


• Study on the Formation Mechanism of Bio-Nanostructures by a Micro-Fluidic Force

  SUZUKI Hiroshi, KOMODA Yoshiyuki, KATSUDA Tomohisa

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2008 - 2010

  In order to investigate the formation mechanism of liposomes in a micro-channel, an experimental study has been performed. The Lagrangian observations and velocity field measurements by a micro-PTV were carried out by use of a movable stage. From the results, it was found that the lipid film pre-coated on the wall were taken into the liquid/air interface and the rod-like liposomes were formed between the interface and the wall and that the rod-like liposomes becomes spherical liposomes in a flow. It was also found that the rod-like liposomes serially induced from the interface by the strong reverse flow existing near the interface


• 産業技術研究「活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発」

  勝田 知尚

  2008


• 昇温溶出アフィニティークロマトグラフィーによる一塩基多型の検出

  勝田 知尚

  2007


• 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発

  勝田 知尚

  2006


• 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発

  勝田 知尚

  2005


• Integrate System for Creation, Detection and Production of Antibodies with Variety of Characteristics

  KATOH Shigeo, KATUSDA Tomohisa, SHIOMI Naofumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2003 - 2004

  In the fields of development of pharmaceuticals, medical diagnosis-analysis and bioprocesses, production of variety of antibodies, detection of the interactions between antibody and protein, screening methods of antibodies and effective production processes of antibodies are essential. In this work, we developed the simple and rapid methods to obtain antibody ligands for recognition and detection of target proteins, based on our recent researches on production of large libraries of antibodies, a high sensitivity detection method of proteins and effective production of antibodies by secretion from yeast cells. 1.A colony lift assay for detection of antibody secreted from E. coli was developed by use of antigen-adsorbed PVDF membrane and liposome immunoblotting assay. By use of this method, high density and micro-assay was possible and the number of samples treated was increased. 2.By use of HRP encapsulating immunoliposomes and hydrophobic membranes, an immunoblotting assay method with two orders of magnitude higher sensitivity, compared with the conventional immunoblotting assay using HRP-conjugated antibody, was developed. 3.A large library of whole antibodies was obtained based on mating of yeast cells transformed by libraries of H- and L-chains, and aproduction method of antibodies by secretion from yeast cells was established. 4.An affinity biopanning method utilizing immunoliposomes was developed and it was applied to biopanning selection with a high efficiency.

• リポソームの製造方法

  SUZUKI HIROSHI, KOMODA YOSHIYUKI, KATSUDA TOMOHISA

  特願2012-553652, 06 Jan. 2012, 大学長, 特許5904555, 25 Mar. 2016

  Patent right


• リポソーム製剤の製造方法

  SUZUKI HIROSHI, KATSUDA TOMOHISA, KATOH SHIGEO, KOMODA YOSHIYUKI, USUI HIROMOTO

  特願2007-134414, 21 May 2007, 大学長, 特許5126874, 09 Nov. 2012

  Patent right