Research activity information

• Dec. 2024 Kobe University, Kobe University Best Teacher Award for General Education


• Nov. 2023 The Kansai Society of Livestock Science, Kansai Society of Animal Science Award, Exploration of Molecular Mechanisms Functioning in Intramuscular Fat and Aroma Formation in Japanese Black Cattle Beef


• Mar. 2023 Kobe University, Rikusoukai Achievement Award


• Jun. 2022 The Ito Foundation, The Ito Foundation Award, 畜産物の品質評価、付加価値の向上に向けたメタボロミクス技術の応用に関する研究

• Single-Nucleus RNA Sequencing Reveals Muscle-Region-Specific Differences in Fibro-Adipogenic Progenitors Driving Intramuscular Fat Accumulation.

  Shuji Ueda, Chiaki Kitamura, Yuka Tateoka, Akinori Kanai, Yutaka Suzuki, Itsuko Fukuda, Yasuhito Shirai

  BACKGROUND: Ectopic fat deposition refers to lipid accumulation that affects metabolic function and tissue characteristics. Japanese Black cattle are distinguished by their high intramuscular fat content, which contributes to their distinctive character. However, the genetic mechanisms underlying these traits remain unclear. This study compared gene expression patterns in different muscle regions to identify genes associated with intramuscular fat accumulation. First, we conducted RNA sequencing to analyze differences in gene expression profiles among the sternocleidomastoid, pectoralis minor, and pectoralis major muscles. In addition, single-cell nuclear RNA sequencing was conducted to investigate the cellular composition of these muscle tissues. RESULTS: Distinct gene expression patterns were observed among the different muscles. In the pectoralis, which contains a high proportion of intramuscular fat, adipocyte-related genes such as FABP4, SCD, and ADIPOQ were highly expressed. In addition, lipases such as PNPLA2, LPL, MGLL, and LIPE were predominantly expressed in intramuscular fat, whereas PLA2G12A, PLD3, and ALOX15 were specifically expressed in myofibers. Moreover, a subclass of fibro-adipogenic progenitor cells that differentiate into intramuscular adipocytes was found to express genes related to microenvironment formation, including ICAM1, TGFBRs, and members of the COL4A family. CONCLUSIONS: This study provides novel insight into the genetic regulation of intramuscular fat accumulation. It improves our understanding of the molecular mechanisms underlying their distinctive meat characteristics.

  Mar. 2025, Metabolites, 15(4) (4), English, International magazine

  Scientific journal


• Metabolites and Free Fatty Acids in Japanese Black Beef During Wet Aging.

  Shuji Ueda, Yuka Yoshida, Yuka Tateoka, Biniam Kebede, Masakazu Shinohara, Hiroki Nakanishi, Itsuko Fukuda, Yasuhito Shirai

  Background: Japanese Black beef is known for its high intramuscular fat content, an important factor in its distinctive Wagyu aroma. Wet aging, which involves vacuum-packing meat and storing it at low temperatures, enhances flavor, texture, and tenderness and is essential for maintaining and improving meat quality. In this study, changes in metabolites and lipid profiles were investigated during the wet aging of Japanese Black and Holstein beef. Methods/Results: Gas chromatography-mass spectrometry identified 113 metabolites in Japanese Black beef and 94 in Holstein beef, with significant increases in metabolites like aspartic acid and maleic acid over the aging period. Regarding lipid composition, total free fatty acids significantly increased with wet aging, with Japanese Black beef showing significantly higher concentrations of oleic and linoleic acids than Holstein beef. Additionally, lipid analysis by liquid chromatography-mass spectrometry revealed a reduction in specific phospholipids, particularly lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), with notable decreases in LPC (18:1), LPC (18:2), LPE (18:1), and LPE (18:2). Conclusions: These results suggest that wet aging influences the stability of membrane lipids, facilitating the degradation of phospholipids into free fatty acids, and improving the flavor of Japanese Black beef.

  Feb. 2025, Metabolites, 15(2) (2), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• New Implications of Metabolites and Free Fatty Acids in Quality Control of Crossbred Wagyu Beef during Wet Aging Cold Storage.

  Shuji Ueda, Yuka Yoshida, Biniam Kebede, Chiaki Kitamura, Ryo Sasaki, Masakazu Shinohara, Itsuko Fukuda, Yasuhito Shirai

  Efficient cold-chain delivery is essential for maintaining a sustainable global food supply. This study used metabolomic analysis to examine meat quality changes during the "wet aging" of crossbred Wagyu beef during cold storage. The longissimus thoracic (Loin) and adductor muscles (Round) of hybrid Wagyu beef, a cross between the Japanese Black and Holstein-Friesian breeds, were packaged in vacuum film and refrigerated for up to 40 days. Sensory evaluation indicated an increase in the umami and kokumi taste owing to wet aging. Comprehensive analysis using gas chromatography-mass spectrometry identified metabolite changes during wet aging. In the Loin, 94 metabolites increased, and 24 decreased; in the Round, 91 increased and 18 decreased. Metabolites contributing to the umami taste of the meat showed different profiles during wet aging. Glutamic acid increased in a cold storage-dependent manner, whereas creatinine and inosinic acid degraded rapidly even during cold storage. In terms of lipids, wet aging led to an increase in free fatty acids. In particular, linoleic acid, a polyunsaturated fatty acid, increased significantly among the free fatty acids. These results provide new insight into the effects of wet aging on Wagyu-type beef, emphasizing the role of free amino acids, organic acids, and free fatty acids generated during cold storage.

  Jan. 2024, Metabolites, 14(2) (2), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Isolation and identification of hyaluronan-degrading bacteria from Japanese fecal microbiota.

  Hazuki Akazawa, Itsuko Fukuda, Haruna Kaneda, Shoichi Yoda, Mamoru Kimura, Ryohei Nomoto, Shuji Ueda, Yasuhito Shirai, Ro Osawa

  Hyaluronan (HA) is a high-molecular-weight glycosaminoglycan and widely distributed in all connective tissues and organs with diverse biological functions. HA has been increasingly used as dietary supplements targeted to joint and skin health for humans. We here first report isolation of bacteria from human feces that are capable of degrading HA to lower molecular weight HA oligosaccharides (oligo-HAs). The bacteria were successfully isolated via a selective enrichment method, in which the serially diluted feces of healthy Japanese donors were individually incubated in an enrichment medium containing HA, followed by the isolation of candidate strains from streaked HA-containing agar plates and selection of HA-degrading strains by measuring HA using an ELISA. Subsequent genomic and biochemical assays identified the strains as Bacteroides finegoldii, B. caccae, B. thetaiotaomicron, and Fusobacterium mortiferum. Furthermore, our HPLC analysis revealed that the strains degraded HA to oligo-HAs of various lengths. Subsequent quantitative PCR assay targeting the HA degrading bacteria showed that their distribution in the Japanese donors varied. The evidence suggests that dietary HA is degraded by the human gut microbiota with individual variation to oligo-HAs components, which are more absorbable than HA, thereby exerting its beneficial effects.

  2023, PloS one, 18(5) (5), e0284517, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Vitamin E functions by association with a novel binding site on the 67 kDa laminin receptor activating diacylglycerol kinase.

  Daiki Hayashi, Varnavas D Mouchlis, Seika Okamoto, Tomoka Namba, Liuqing Wang, Sheng Li, Shuji Ueda, Minoru Yamanoue, Hirofumi Tachibana, Hiroyuki Arai, Hitoshi Ashida, Edward A Dennis, Yasuhito Shirai

  It is generally recognized that the main function of α-tocopherol (αToc), which is the most active form of vitamin E, is its antioxidant effect, while non-antioxidant effects have also been reported. We previously found that αToc ameliorates diabetic nephropathy via diacylglycerol kinase alpha (DGKα) activation in vivo, and the activation was not related to the antioxidant effect. However, the underlying mechanism of how αToc activates DGKα have been enigmatic. We report that the membrane-bound 67 kDa laminin receptor (67LR), which has previously been shown to serve as a receptor for epigallocatechin gallate (EGCG), also contains a novel binding site for vitamin E, and its association with Vitamin E mediates DGKα activation by αToc. We employed hydrogen-deuterium exchange mass spectrometry (HDX/MS) and molecular dynamics (MD) simulations to identify the specific binding site of αToc on the 67LR and discovered the conformation of the specific hydrophobic pocket that accommodates αToc. Also, HDX/MS and MD simulations demonstrated the detailed binding of EGCG to a water-exposed hydrophilic site on 67LR, while in contrast αToc binds to a distinct hydrophobic site. We demonstrated that 67LR triggers an important signaling pathway mediating non-antioxidant effects of αToc, such as DGKα activation. This is the first evidence demonstrating a membrane receptor for αToc and one of the underlying mechanisms of a non-antioxidant function for αToc.

  Dec. 2022, The Journal of nutritional biochemistry, 110, 109129 - 109129, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5.

  Shuji Ueda, Moeka Nishihara, Yuuki Hioka, Ken-Ichi Yoshino, Soichiro Yamada, Minoru Yamanoue, Yasuhito Shirai

  Protein homeostasis, including protein folding, refolding, and degradation, is thought to decline with aging. HSPB5 (also known as αB-crystallin) prevents target protein aggregation as a molecular chaperone and exhibits a cytoprotective function against various cell stresses. To elucidate the effect of HSPB5 on endoplasmic reticulum (ER) stress, we searched for novel binding proteins of HSPB5 using the proximity-dependent biotin labeling method. Proteins presumed to interact with HSPB5 in cells treated with the proteasome inhibitor MG132 were identified by a reversible biotin-binding capacity method combining tamavidin2-REV magnetic beads and mass spectrometry. We discovered a new binding protein for HSPB5, polo-like kinase 2 (PLK2), which is an apoptosis-related enzyme. The expression of PLK2 was upregulated by MG132 treatment, and it was co-localized with HSPB5 near the ER in L6 muscle cells. Inhibition of PLK2 decreased ER stress-induced phosphorylation of serine 19 in HSPB5 and increased apoptosis by activation of caspase 3 under ER stress. Overexpression of HSPB5 (WT) suppressed the ER stress-induced caspase 3 activity, but this was not observed with phospho-deficient HSPB5 (3A) mutants. These results clarify the role of HSPB5 phosphorylation during ER stress and suggest that the PLK2/HSPB5 pathway plays an essential role in cytoprotection against proteasome inhibition-induced ER stress.

  Sep. 2022, International journal of molecular sciences, 23(19) (19), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef.

  Shuji Ueda, Yasuharu Takashima, Yunosuke Gotou, Ryo Sasaki, Rio Nakabayashi, Takeshi Suzuki, Shinji Sasazaki, Ituko Fukuda, Biniam Kebede, Yuki Kadowaki, Maiko Tamura, Hiroki Nakanishi, Yasuhito Shirai

  Japanese Black cattle (Japanese Wagyu) beef is attracting attention for its aroma and marbling, and its handling is increasing worldwide. Here, we focused on the origin discrimination of Wagyu beef and analyzed the nutritional components of Japanese Wagyu (produced in multiple prefectures of Japan), Hybrid Wagyu (a cross between Angus and Wagyu cattle born in Australia and transported to Japan), and Australian Wagyu beef using mass spectrometry (MS). Triple-quadrupole liquid chromatography-MS was used to clarify the molecular species of lipids in Wagyu beef. Fourteen classes of lipids were separated, and 128 different triacylglycerides (TGs) were detected. A simple comparative analysis of these TGs using high-performance liquid chromatography revealed significantly higher levels of triolein (C18:1/C18:1/C18:1; abbreviated OOO) and C18:1/C18:1/C16:1 (OOPo) in Japanese Wagyu. Wagyu elements beef were comprehensively analyzed using inductively coupled plasma (ICP)-MS and ICP-optical emission spectrometry. We found significant differences in the rubidium, cesium, and lithium levels of Japanese and Australian Wagyu beef. On comparing metabolites using gas chromatography-MS, we identified significant differences in the levels of amino acids and other components of the Japanese and Australian Wagyu beef. These results suggest the possibility of determining the origin of Wagyu cattle breeds using MS and genetic discrimination.

  Aug. 2022, Metabolites, 12(9) (9), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of Hydroxy Fatty Acids, Precursors of γ-Hexalactone, Contributes to the Characteristic Sweet Aroma of Beef.

  Shuji Ueda, Mana Hosoda, Kumi Kasamatsu, Masahiro Horiuchi, Rio Nakabayashi, Bubwoong Kang, Masakazu Shinohara, Hiroki Nakanishi, Takayo Ohto-Nakanishi, Minoru Yamanoue, Yasuhito Shirai

  Aroma is an essential factor for meat quality. The meat of Japanese Black cattle exhibits fine marbling and a rich and sweet aroma with a characteristic lactone composition. The mechanism of lactone formation associated with beef aroma has not been elucidated. In this study, we examined the precursors of γ-hexalactone, an indicator of the sweet aroma of beef and identified the mechanism underlying γ-hexalactone production. A low-temperature vacuum system was used to prepare beef tallow from Japanese Black cattle and Holstein cattle. The odor components were identified using headspace-gas chromatography. The analysis revealed that γ-hexalactone, γ-dodecalactone, δ-tetradecalactone, and δ-hexadecalactone were present as sweet aroma components of beef tallow prepared from marbling and muscle. Since we previously reported that γ-hexalactone formation correlates with linoleic acid content in beef, we analyzed ten oxidized fatty acids derived from linoleic acid by liquid chromatography-triple quadrupole mass spectrometry and detected two hydroxy-octadecadienoic acids (9S-HODE and 13S-HODE) in beef tallow. Significant differences in arachidonic acid 15-lipoxygenase and cyclooxygenase protein expression levels among subcutaneous fat, intramuscular fat, and muscle tissue were observed. Our results suggest that the combination of linoleic acid and the expression of lipid oxidase derived from beef muscle and intramuscular fat produce hydroxy fatty acids that result in a sweet aroma.

  Apr. 2022, Metabolites, 12(4) (4), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• RalA, PLD and mTORC1 Are Required for Kinase-Independent Pathways in DGKβ-Induced Neurite Outgrowth.

  Takuya Kano, Ryosuke Tsumagari, Akio Nakashima, Ushio Kikkawa, Shuji Ueda, Minoru Yamanoue, Nobuyuki Takei, Yasuhito Shirai

  Diacylglycerol kinase β (DGKβ) is an enzyme that converts diacylglycerol to phosphatidic acid and is mainly expressed in the cerebral cortex, hippocampus and striatum. We previously reported that DGKβ induces neurite outgrowth and spinogenesis, contributing to higher brain functions, including emotion and memory. To elucidate the mechanisms involved in neuronal development by DGKβ, we investigated the importance of DGKβ activity in the induction of neurite outgrowth using human neuroblastoma SH-SY5Y cells. Interestingly, both wild-type DGKβ and the kinase-negative (KN) mutant partially induced neurite outgrowth, and these functions shared a common pathway via the activation of mammalian target of rapamycin complex 1 (mTORC1). In addition, we found that DGKβ interacted with the small GTPase RalA and that siRNA against RalA and phospholipase D (PLD) inhibitor treatments abolished DGKβKN-induced neurite outgrowth. These results indicate that binding of RalA and activation of PLD and mTORC1 are involved in DGKβKN-induced neurite outgrowth. Taken together with our previous reports, mTORC1 is a key molecule in both kinase-dependent and kinase-independent pathways of DGKβ-mediated neurite outgrowth, which is important for higher brain functions.

  Dec. 2021, Biomolecules, 11(12) (12), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gene Expression Analysis Provides New Insights into the Mechanism of Intramuscular Fat Formation in Japanese Black Cattle

  Shuji Ueda, Mana Hosoda, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai

  Japanese Black cattle (Japanese Wagyu) have a unique phenotype in which ectopic intramuscular fat accumulates in skeletal muscle, producing finely marbled beef. However, the mechanism of intramuscular fat formation in Japanese Black cattle remains unclear. To investigate the key genes involved in intramuscular fat accumulation, we comprehensively analyzed mRNA levels in subcutaneous and intramuscular fat tissues using RNA sequence (RNA-seq) analysis, which detected 27,606 genes. We identified eight key genes, namely carboxypeptidase E, tenascin C, transgelin, collagen type IV alpha 5 (COL4A5), cysteine and glycine-rich protein 2, PDZ, and LIM domain 3, phosphatase 1 regulatory inhibitor subunit 14A, and regulator of calcineurin 2. These genes were highly and specifically expressed in intramuscular fat tissue. Immunohistochemical analysis revealed a collagen network, including COL4A5, in the basement membrane around the intramuscular fat tissue. Moreover, pathway analysis revealed that, in intramuscular fat tissue, differentially expressed genes are related to cell adhesion, proliferation, and cancer pathways. Furthermore, pathway analysis showed that the transforming growth factor-β (TGF-β) and small GTPases regulators RASGRP3, ARHGEF26, ARHGAP10, ARHGAP24, and DLC were upregulated in intramuscular fat. Our study suggests that these genes are involved in intramuscular fat formation in Japanese Black cattle.

  MDPI AG, Jul. 2021, Genes, 12(8) (8), 1107 - 1107

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

  Joleen S Cheah, Kyle A Jacobs, Tzu Wei Lai, Reca Caballelo, Jacqueline L Yee, Shuji Ueda, Volkmar Heinrich, Soichiro Yamada

  Sensing physical forces is a critical first step in mechano-transduction of cells. Zyxin, a LIM domain-containing protein, is recruited to force-bearing actin filaments and is thought to repair and strengthen them. Yet, the precise force-induced protein interactions surrounding zyxin remain unclear. Using BioID analysis, we identified proximal proteins surrounding zyxin under normal and force-bearing conditions by label-free mass spectrometry analysis. Under force-bearing conditions, increased biotinylation of α-actinin 1, α-actinin 4, and AFAP1 were detected, and these proteins accumulated along force-bearing actin fibers independently from zyxin, albeit at a lower intensity than zyxin. VASP also accumulated along force-bearing actin fibers in a zyxin-dependent manner, but the biotinylation of VASP remained constant regardless of force, supporting the model of a free zyxin-VASP complex in the cytoplasm being corecruited to tensed actin fibers. In addition, ARHGAP42, a RhoA GAP, was also identified as a proximal protein of zyxin and colocalized with zyxin along contractile actin bundles. The overexpression of ARHGAP42 reduced the rate of small wound closure, a zyxin-dependent process. These results demonstrate that the application of proximal biotinylation can resolve the proximity and composition of protein complexes as a function of force, which had not been possible with traditional biochemical analysis.

  Jun. 2021, Molecular biology of the cell, 32(13) (13), 1221 - 1228, English, International magazine

  Scientific journal


• Exploring the Lipids Involved in the Formation of Characteristic Lactones in Japanese Black Cattle.

  Shuji Ueda, Ryo Sasaki, Rio Nakabayashi, Minoru Yamanoue, Yasuhito Sirai, Eiji Iwamoto

  The meat from Japanese Black cattle (Japanese Wagyu) is finely marbled and exhibits a rich and sweet aroma known as Wagyu beef aroma. To clarify the key metabolites involved in the aroma, we analyzed the correlation between lactone and lipid composition in Japanese Black cattle. Using gas chromatography-olfactometry, we identified 39 characteristic odorants of the intermuscular fat. Seven characteristic lactones considered to be involved in Wagyu beef aroma were quantified and compared in the marbled area and intermuscular fat using a stable isotope dilution assay. Among them, γ-hexalactone was the only lactone whose level was significantly higher in the marbled area. To explore the lipid species involved in lactone formation, we analyzed samples with different aroma characteristics. Liquid chromatography-mass spectrometry revealed eight lipid classes and showed significant differences in triacylglycerides (TAGs). To determine the molecular species of TAGs, we performed high-performance liquid chromatography analysis and identified 14 TAG species. However, these analyses showed that seven lactones had a low correlation with the TAGs. However, γ-hexalactone showed a positive correlation with linoleic acid. This study suggests that lipid composition affects the characteristic lactone profile involved in the Wagyu beef aroma.

  Mar. 2021, Metabolites, 11(4) (4), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Motor Dyscoordination and Alteration of Functional Correlation Between DGKγ and PKCγ in Senescence-Accelerated Mouse Prone 8 (SAMP8)

  Ryosuke Tsumagari, Kenta Maruo, Takaaki Nakao, Shuji Ueda, Minoru Yamanoue, Yasuhito Shirai

  Senescence-accelerated mouse prone 8 (SAMP8) is an animal model of age-related central nervous system (CNS) disorders. Although SAMP8 shows deficits in learning, memory, and emotion, its motor coordination has not been clarified. We have recently reported that DGKγ-regulated PKCγ activity is important for cerebellar motor coordination. However, involvement of the functional correlation between the kinases in age-related motor dyscoordination still remains unknown. Therefore, we have investigated the motor coordination in SAMP8 and involvement of the functional correlation between DGKγ and PKCγ in the age-related motor dyscoordination. Although 6 weeks old SAMP8 showed equivalent motor coordination with control mice (SAMR1) in the rotarod test, 24 weeks old SAMP8 exhibited significantly less latency in the rotarod test and more frequent slips in the beam test compared to the age-matched SAMR1. Furthermore, 24 weeks old SAMP8 showed the higher locomotor activity in open field test and Y-maze test. Western blotting revealed that DGKγ expression decreased in the cerebellum of 24 weeks old SAMP8, while PKCγ was upregulated. These results suggest that SAMP8 is a useful model of age-related motor dysfunction and that the DGKγ-regulated PKCγ activity is involved in the age-related motor dyscoordination.

  Frontiers Media SA, Jan. 2021, Frontiers in Aging Neuroscience, 13

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Exploring the Characteristic Aroma of Beef from Japanese Black Cattle (Japanese Wagyu) via Sensory Evaluation and Gas Chromatography-Olfactometry.

  Shuji Ueda, Minoru Yamanoue, Yasuhito Sirai, Eiji Iwamoto

  Beef from Japanese Black cattle (Japanese Wagyu) is renowned for its flavor characteristics. To clarify the key metabolites contributing to this rich and sweet aroma of beef, an omics analysis combined with GC-olfactometry (GC-O) and metabolomics analysis with gas chromatography-mass spectrometry (GC-MS) were applied. GC-O analysis identified 39 odor-active odorants from the volatile fraction of boiled beef distilled by solvent-assisted flavor evaporation. Eight odorants predicted to contribute to Wagyu beef aroma were compared between Japanese Black cattle and Holstein cattle using a stable isotope dilution assay with GC-tandem quadrupole mass spectrometry. By correlating the sensory evaluation values of retronasal aroma, γ-hexalactone, γ-d2ecalactone, and γ-undecalactone showed a high correlation with the Wagyu beef aroma. Metabolomics data revealed a high correlation between the amounts of odorants and multiple metabolites, such as glutamine, decanoic acid, lactic acid, and phosphoric acid. These results provide useful information for assessing the aroma and quality of beef.

  Jan. 2021, Metabolites, 11(1) (1), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Protective effect of Zingiber zerumbet Smith extract on thermotolerance

  Yasuhito Shirai, Hisakazu Kobayashi, Shuji Ueda, Yun Sang Soon, Akiho Kushiya, Ryosuke Tsumagari, Minoru Yamanoue

  Background: Global warming causes severe heat conditions. Heat stress contributes to higher morbidity of heatstroke in human and mortality in livestock. To protect them from heat stress, thermotolerance mechanisms were widely studied, and some studies suggest relationship between heat shock proteins (HSPs) and thermotolerance. HSPs were not induced by only heat shock but also some stimulations including bioactive compounds from plants. Zingiber zerumbet is a perennial herb found in many tropical countries, including Thailand. The rhizome of Zingiber zerumbet contains zerumbone that is a bioactive compound to induce HSPs expression in animal cells.Objective: To prevent higher morbidity of heatstroke in human and mortality in livestock by the heat stress, we investigated the effect of zerumbone, the extract of Zingiber zerumbet Smith, on thermotolerance, using a cell line and mice.Methods: The murine liver hepatoma cell line, Hepa1c1c7 cells, were incubated in medium supplemented with extract from rhizome of Zingiber zerumbet Simith containing zerumbone, and then the expression of heat shock proteins (HSP) 40, 70 and 90 were investigated by western blotting. Furthermore, we established the evaluation system of thermotolerance using mice, and studied the effect of the extract on the growth rate of mice under the heat shock treatment. Briefly, 4 weeks old C57BL6 mice were fed that with the extract (or vehicle) for a week before the first heat shock treatment (38 °C for an hour). Before and after five days heat treatment, body weights were measured. The protein expressions of heat shock proteins in liver were measured by western blotting using HSPs antibodies.Results: The extract of Zingiber zerumbet rhizome, equivalent to 50 μM zerumbone, significantly increased the expression of heat shock proteins (HSP40, HSP70, HSP90). The growth rate of the mice under the heat treatment were lower than control. The feeding with the extract containing 25 ppm zerumbone have significantly attenuated the decline of the growth rate led by the heat treatment, whereas there was little effect on mouse growth rate grown under normal conditions. The protein expression of HSP70 in the liver of zerumbone-fed mice was upregulated compared with control mice, equivalent to heat treatment without zerumbone. On the other hand, both treatments of zerumbone and heat resulted in highest HSP70 expression among four groups.Conclusion: Our study demonstrated that oral administration of the extract of Zingiber zerumbet Smith led to the attenuation of decline of growth rate induced by heat treatment. HSP70 expression in murine liver was enhanced by either feeding the extract or heat treatment. More interestingly, HSP70 expression was further enhanced by both treatments of zerumbone and heat. These results suggested that zerumbone may contribute to thermotolerance via, at least, HSP70 expression.Keywords: Zingiber zerumbet, thermotolerance, heat shock protein

  Functional Food Center, Jan. 2021, Bioactive Compounds in Health and Disease, 4(1) (1), 1 - 1

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The mechanisms of ameliorating effect of a green tea polyphenol on diabetic nephropathy based on diacylglycerol kinase α

  Daiki Hayashi, Liuqing Wang, Shuji Ueda, Minoru Yamanoue, Hitoshi Ashida, Yasuhito Shirai

  Significant efforts have been made to ameliorate diabetic nephropathy (DN) by inhibiting protein kinase C. However, these efforts have not been successful in human trials, suggesting that novel therapeutic strategies are required. Thus far, it has been reported that green tea polyphenol epigallocatechin gallate (EGCg) improved albuminuria in DN in a human trial. Our previous study revealed that activation of diacylglycerol kinase α (DGKα) plays a crucial role in the amelioration of DN and that EGCg activates DGKα. Here, we investigated whether and how DGKα contributes to the amelioration of DN upon stimulation by EGCg by using streptozotocin-induced type 1 diabetic model mice. Our results revealed that EGCg ameliorated albuminuria in DN through DGKα in vivo, and methylated EGCg, which has higher absorption in the plasma improved albuminuria in DN effectively. Additionally, we showed that c-Src mediated EGCg-induced DGKα translocation and colocalized with the 67 kDa laminin receptor, which is an EGCg receptor. Furthermore, EGCg attenuated the loss of podocytes in DN by preventing a decrease in focal adhesion under high glucose conditions. Our results indicate that the DGKα pathway is an attractive therapeutic target and that activating this pathway is a novel strategy for treating DN.

  Springer Science and Business Media LLC, Dec. 2020, Scientific Reports, 10(1) (1)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Precise Regulation of the Basal PKCγ Activity by DGKγ Is Crucial for Motor Coordination.

  Ryosuke Tsumagari, Kenta Maruo, Sho Kakizawa, Shuji Ueda, Minoru Yamanoue, Hiromitsu Saito, Noboru Suzuki, Yasuhito Shirai

  Diacylglycerol kinase γ (DGKγ) is a lipid kinase to convert diacylglycerol (DG) to phosphatidic acid (PA) and indirectly regulates protein kinase C γ (PKCγ) activity. We previously reported that the basal PKCγ upregulation impairs cerebellar long-term depression (LTD) in the conventional DGKγ knockout (KO) mice. However, the precise mechanism in impaired cerebellar LTD by upregulated PKCγ has not been clearly understood. Therefore, we first produced Purkinje cell-specific DGKγ KO (tm1d) mice to investigate the specific function of DGKγ in Purkinje cells and confirmed that tm1d mice showed cerebellar motor dysfunction in the rotarod and beam tests, and the basal PKCγ upregulation but not PKCα in the cerebellum of tm1d mice. Then, the LTD-induced chemical stimulation, K-glu (50 mM KCl + 100 µM, did not induce phosphorylation of PKCα and dissociation of GluR2 and glutamate receptor interacting protein (GRIP) in the acute cerebellar slices of tm1d mice. Furthermore, treatment with the PKCγ inhibitor, scutellarin, rescued cerebellar LTD, with the phosphorylation of PKCα and the dissociation of GluR2 and GRIP. In addition, nonselective transient receptor potential cation channel type 3 (TRPC3) was negatively regulated by upregulated PKCγ. These results demonstrated that DGKγ contributes to cerebellar LTD by regulation of the basal PKCγ activity.

  Oct. 2020, International journal of molecular sciences, 21(21) (21), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 67kDaラミニンレセプターはビタミンEとの結合によりパルミトイル化修飾を受ける

  岡本 聖香, 林 大輝, 足立 直子, Varnavas Mouchlis, 上田 修司, 山之上 稔, Dennis Edward, 斎藤 尚亮, 伊藤 俊樹, 白井 康仁

  (公社)日本生化学会, Sep. 2020, 日本生化学会大会プログラム・講演要旨集, 93回, [1Z09 - 161)], Japanese


• MEATabolomics: Muscle and Meat Metabolomics in Domestic Animals.

  Susumu Muroya, Shuji Ueda, Tomohiko Komatsu, Takuya Miyakawa, Per Ertbjerg

  In the past decades, metabolomics has been used to comprehensively understand a variety of food materials for improvement and assessment of food quality. Farm animal skeletal muscles and meat are one of the major targets of metabolomics for the characterization of meat and the exploration of biomarkers in the production system. For identification of potential biomarkers to control meat quality, studies of animal muscles and meat with metabolomics (MEATabolomics) has been conducted in combination with analyses of meat quality traits, focusing on specific factors associated with animal genetic background and sensory scores, or conditions in feeding system and treatments of meat in the processes such as postmortem storage, processing, and hygiene control. Currently, most of MEATabolomics approaches combine separation techniques (gas or liquid chromatography, and capillary electrophoresis)-mass spectrometry (MS) or nuclear magnetic resonance (NMR) approaches with the downstream multivariate analyses, depending on the polarity and/or hydrophobicity of the targeted metabolites. Studies employing these approaches provide useful information to monitor meat quality traits efficiently and to understand the genetic background and production system of animals behind the meat quality. MEATabolomics is expected to improve the knowledge and methodologies in animal breeding and feeding, meat storage and processing, and prediction of meat quality.

  May 2020, Metabolites, 10(5) (5), English, International magazine

  Scientific journal


• mTORC1 is involved in DGKβ-induced neurite outgrowth and spinogenesis.

  Hiroko Nakai, Ryosuke Tsumagari, Kenta Maruo, Akio Nakashima, Ushio Kikkawa, Shuji Ueda, Minoru Yamanoue, Naoaki Saito, Nobuyuki Takei, Yasuhito Shirai

  Diacylglycerol kinase β (DGKβ) is an enzyme converting DG to phosphatidic acid (PA) and is specifically expressed in neurons, especially those in the cerebral cortex, hippocampus and striatum. We previously reported that DGKβ induces neurite outgrowth and spinogenesis, contributing to higher brain function including emotion and memory, and plasma membrane localization of DGKβ via the C1 domain and a cluster of basic amino acids at the C-terminus is necessary for its function. To clarify the mechanisms involved in neuronal development by DGKβ, we investigated whether DGKβ activity induces neurite outgrowth using human neuroblastoma SH-SY5Y cells. DGKβ induced neurite outgrowth by activation of mammalian target of rapamycin complex 1 (mTORC1) through a kinase-dependent pathway. In addition, in primary cultured cortical and hippocampal neurons, inhibition of mTORC1 abolished DGKβ induced-neurite outgrowth, branching and spinogenesis. These results indicated that DGKβ induces neurite outgrowth and spinogenesis by activating mTORC1 in a kinase-dependent pathway.

  Mar. 2020, Neurochemistry international, 134, 104645 - 104645, English, International magazine

  [Refereed]

  Scientific journal


• Comparative Study of the Effects of Different Pretreatment Procedures on Beef Taste-traits Using an Electronic Taste Sensing System

  Yanan Zhao, Masahiro Nishida, Shuji Ueda, Yasuhito Shirai, Masaaki Habara, Hidekazu Ikezaki, Minoru Yamanoue

  Japanese Society for Food Science and Technology, 2020, Food Science and Technology Research, 26(3) (3), 329 - 338

  Scientific journal


• Dgkg knock-out mice show impairments in cerebellar motor coordination, Ltd, and the dendritic development of purkinje cells through the activation of pkcg

  Ryosuke Tsumagari, Sho Kakizawa, Sakiko Kikunaga, Yoshitaka Fujihara, Shuji Ueda, Minoru Yamanoue, Naoaki Saito, Masahito Ikawa, Yasuhito Shirai

  2020, eNeuro, 7(2) (2)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 低親和性ビオチン結合タンパク質Tamavidinを用いた新規ペプチド捕捉型BioID法の開発

  吉野 健一, 西原 萌華, 上田 修司, 竹内 敦子

  日本組織細胞化学会, Sep. 2019, 日本組織細胞化学会総会・学術集会講演プログラム・予稿集, 60回, 97 - 97, Japanese


• Screening of subtype-specific activators and inhibitors for diacylglycerol kinase.

  Hayashi D, Tsumagari R, Liu K, Ueda S, Yamanoue M, Sakane F, Shirai Y

  Jun. 2019, Journal of biochemistry, 165(6) (6), 517 - 522, English

  [Refereed]

  Scientific journal


• Comparative metabolomics of Japanese Black cattle beef and other meats using gas chromatography–mass spectrometry

  UEDA SyUJI, IWAMOTO EIJI, KATO YOSHIKI, SHINOHARA MASAKAZU, SHIRAI YASUHITO, YAMANOUE MINORU

  Progress in metabolomic analysis now allows the evaluation of food quality. This study aims to identify the metabolites in meat from livestock using a metabolomic approach. Using gas chromatography-mass spectrometry (GC/MS), many metabolites were reproducibly detected in meats, and distinct differences between livestock species (cattle, pigs, and chickens) were indicated. A comparison of metabolites between tissues types (muscle, intramuscular fat, and intermuscular fat) in marbled beef of Japanese Black cattle revealed that most metabolites are abundant in the muscle tissue. Several metabolites (medium-chain fatty acids, etc.) involved in triacylglycerol synthesis were uniquely detected in fat tissue. Additionally, the results of multivariate analysis suggest that GC/MS analysis of metabolites can distinguish between cattle breeds. These results provide useful information for the analysis of meat quality using GC/MS-based metabolomic analysis.ABBREVIATIONS: GC/MS: gas chromatography-mass spectrometry; NMR: nuclear magnetic resonance; MS: mass spectrometry; IS: 2-isopropylmalic acid; MSTFA: N-Methyl-N-trimethylsilyltrifluoroacetamide; CV: coefficient of variation; TBS: Tris-buffered saline; MHC: myosin fast type; PCA: principal component analysis; OPLS-DA: orthogonal partial least-squares discriminant analysis; O2PLS: two-way orthogonal partial least-squares.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Jan. 2019, Bioscience, Biotechnology, and Biochemistry, 83(1) (1), 137 - 147, English, International magazine

  [Refereed]

  Scientific journal


• Comparative metabolomics of Japanese Black cattle beef and other meats using gas chromatography-mass spectrometry.

  UEDA SHUJI, Iwamoto Eiji, Kato Yoshiki, Shinohara Masakazu, Shirai Yasuhito, Yamanoue Minoru

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Oct. 2018, Bioscience, Biotechnology, and Biochemistry, 83, 137 - 147, English

  [Refereed]

  Scientific journal


• RhoA結合蛋白質として同定されたSTE20 like kinaseの筋細胞の肥大化における役割

  櫛谷 晃帆, 上田 修司, 前田 愛実, 加藤 良毅, 吉野 健一, 竹内 敦子, 山之上 稔, 白井 康仁

  (公社)日本生化学会, Sep. 2018, 日本生化学会大会プログラム・講演要旨集, 91回, [1T14e - 370)], Japanese


• Structural difference between myofibrillar protein, paratropomyosin, and tropomyosin as revealed by high-performance liquid chromatography

  NISHIO Yuriko, USHIMURA Yuichi, UEDA Shuji, MAEDA Naoyuki, HATTORI Akihito, YAMANOUE MINORU

  Paratropomyosin (PTM) composes myofibril functions to weaken the rigor linkages formed between actin and myosin during postmortem aging of muscles. PTM has the similar physico-chemical properties as tropomyosin (TM) that is a regulatory protein of myofibrils. So far, it is unclear whether PTM is definitely different from TM, because the primary structure of PTM has not been determined yet. The aim of this study was to clarify structural difference of PTM from TM. PTM was prepared by column chromatography immediately after slaughter from broiler breast muscle, and purified by high-performance liquid chromatography (HPLC). Purified PTM was successfully separated from TM, and the recovered PTM molecule was reduced with dithiothreitol to separate again by HPLC. Two subunits were obtained and peptides from each digested subunit by V8 protease were recovered by HPLC, and then amino acid sequences of the peptides were analyzed by protein sequencing. As a result, some amino acid residues were replaced from that of TMα1 isoform which is the major isoform of TM, and also was different between the two subunits. Therefore, it is concluded that PTM clearly differs from TM and it is suggested that functional difference in PTM from TM is attributed to amino acid replacements in subunits composing PTM.

  Aug. 2018, Animal Science Journal, 89(8) (8), 1161 - 1168, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Epigallocatechin gallate induces GLUT4 translocation in skeletal muscle through both PI3K- and AMPK-dependent pathways.

  UEDA-WAKAGI Manabu, HAYASHIBARA Kaori, NAGANO Tomoya, IKEDA Masaki, YUAN Sihao, UEDA Shuji, SHIRAI Yasuhito, YOSHIDA Ken-ichi, ASHIDA Hitoshi

  Our previous report demonstrated that epigallocatechin gallate (EGCg) promotes translocation of glucose transporter 4 (GLUT4) in skeletal muscle. In this study, we investigated the molecular mechanism of GLUT4 translocation by EGCg at the physiological concentration range. In L6 cells, EGCg induced phosphorylation of phosphatidylinositide 3'-kinase (PI3K) and downstream protein kinase C (PKC) λ/ξ without affecting the phosphorylation of insulin receptor and Akt. EGCg-induced GLUT4 translocation was suppressed by RNA interference-mediated knockdown of PI3K and treatment with PKC inhibitor Go6983. Moreover, EGCg increased Rac1 activity and actin remodelling as downstream events of PKCλ/ξ. These results indicate that EGCg induced GLUT4 translocation through a PI3K-dependent pathway, but its mode of action differed from that of insulin. EGCg also induced GLUT4 translocation through a 5'-adenosine monophosphate-activated protein kinase (AMPK)-dependent pathway. 67 kDa laminin receptor, which is a target molecule of EGCg, was not involved in EGCg-induced glucose uptake in L6 cells. The oral administration of EGCg suppressed postprandial hyperglycaemia accompanied by GLUT4 translocation through both PI3K- and AMPK-dependent pathways, and promoted glycogen accumulation in skeletal muscle of ICR mice. EGCg promotes GLUT4 translocation through both PI3K- and AMPK-dependent pathways and glycogen accumulation in skeletal muscle.

  2018, Food and Function, 9(8) (8), 4223 - 4233, English, International magazine

  [Refereed]

  Scientific journal


• Amelioration of diabetic nephropathy by oral administration of d-α-Tocopherol and its mechanisms

  Daiki Hayashi, Shuji Ueda, Minoru Yamanoue, Hitoshi Ashida, Yasuhito Shirai

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2018, Bioscience, Biotechnology and Biochemistry, 82(1) (1), 65 - 73, English

  [Refereed]

  Scientific journal


• Diacylglycerol Kinase alpha is Involved in the Vitamin E-Induced Amelioration of Diabetic Nephropathy in Mice.

  Hayashi D, Yagi K, Song C, Ueda S, Yamanoue M, Topham M, Suzaki T, Saito N, Emoto N, Shirai Y

  Jul. 2017, Sci Rep., 7(1) (1), 2597, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of intramuscular FFAs on taste-traits of Japanese Black Wagyu beef

  ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SyUJI, IHARA IKKO, TOYODA KIYOHIKO, YAMANOUE MINORU

  King Mongkut's Institute of Technology Ladkrabang, Aug. 2016, Proceedings of the 62nd ICoMST, 1, O - 05-1-4, English

  [Refereed]

  International conference proceedings


• Detection of the connectin/titin 20-kda fragment increased in chicken sarcoplasm during postmortem aging

  Azusa Naka, Masaya Maruyama, Minami Nakama, Shuji Ueda, YAMANOUE MINORU

  Japanese Society of Animal Science, Aug. 2016, Proceedings of the 17th AAAP Animal Science Congress, 1, 1119 - 1122, English

  [Refereed]

  International conference proceedings


• 熟成による牛肉の脂肪および呈味性の変化

  YAMANOUE MINORU, ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SyUJI, IHARA IKKO, TOYODA KIYOHIKO

  日本食肉研究会, Jun. 2016, 食肉の科学, 57(1) (1), 142 - 144, Japanese

  Symposium


• Epigallocatechin-3-gallate activates diacylglycerol kinase alpha via a 67 kDa laminin receptor: A possibility of galloylated catechins as functional food to prevent and/or improve diabetic renal dysfunctions

  Daiki Hayashi, Shuji Ueda, Minoru Yamanoue, Naoki Saito, Hitoshi Ashida, Yasuhito Shirai

  May 2015, JOURNAL OF FUNCTIONAL FOODS, 15, 561 - 569, English

  [Refereed]

  Scientific journal


• Force dependent biotinylation of myosin IIA by alpha-catenin tagged with a Promiscuous Biotin Ligase

  S. Ueda, A. M. Blee, K. G. Macway, D. J. Renner, S. Yamada

  2015, MOLECULAR BIOLOGY OF THE CELL, 26(10(3)) (10(3)), e0122886, English

  [Refereed]

  Scientific journal


• Chicken heat shock protein HSPB1 increases and interacts with alpha B-crystallin in aged skeletal muscle

  Shuji Ueda, Yoshito Kokaji, Shunsaku Simizu, Kazuhisa Honda, Ken-ichi Yoshino, Hiroshi Kamisoyama, Yasuhito Shirai, Minoru Yamanoue

  2015, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(11) (11), 1867 - 1875, English

  [Refereed]

  Scientific journal


• 脂質結合ドメインを用いた新規ホスファチジン酸可視化プローブの開発

  OKIMOTO KO, NAKAI HIROKO, ITOH TOSHIKI, UEDA SHUJI, YAMANOUE MINORU, SHIRAI YASUHITO

  Dec. 2014, 日本農芸化学会関西支部講演会講演要旨集, 487th, 11, Japanese

  Research society


• Effects of storage and cooking on free fatty acid in Japanese Black Wagyu beef broth

  YAMANOUE MINORU, NISHIDA MASAHIRO, YAMATO SAYAKA, UEDA SyUJI, IHARA IKKO, TOYODA KIYOHIKO

  Nov. 2014, Proc. 16th AAAP Animal Science Congress, English

  [Refereed]

  International conference proceedings


• 筋細胞におけるHSPB1の結合分子の探索とその機能解析

  小鍛治 泰斗, 上田 修司, 吉野 健一, 山之上 稔, 白井 康仁

  (公社)日本生化学会, Oct. 2014, 日本生化学会大会プログラム・講演要旨集, 87回, [4P - 154], Japanese


• Both the C1 domain and a basic amino acid cluster at the C-terminus are important for the neurite and branch induction ability of DGK beta

  Takuya Kano, Takeshi Kouzuki, Satoru Mizuno, Shuji Ueda, Minoru Yamanoue, Fumio Sakane, Naoaki Saito, Yasuhito Shirai

  Apr. 2014, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 447(1) (1), 89 - 94, English

  [Refereed]

  Scientific journal


• The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos

  Shuji Ueda, Becky Tu-Sekine, Minoru Yamanoue, Daniel M. Raben, Yasuhito Shirai

  Oct. 2013, BMC DEVELOPMENTAL BIOLOGY, 13, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of RalA downstream of Rac1 in insulin-dependent glucose uptake in muscle cells.

  Shinsuke Nozaki, Shuji Ueda, Nobuyuki Takenaka, Tohru Kataoka, Takaya Satoh

  The small GTPase RalA has been implicated in glucose uptake in insulin-stimulated adipocytes, although it remains unclear whether RalA has a similar role in insulin signaling in other types of cells. Recently, we have demonstrated that the Rho family GTPase Rac1 has a critical role in insulin-dependent glucose uptake in myoblast culture and mouse skeletal muscle. However, the mechanisms underlying Rac1-dependent glucose uptake, mostly mediated by the plasma membrane translocation of the glucose transporter GLUT4, remain largely unknown. The purpose of this study is to examine the involvement of RalA in Rac1 regulation of the translocation of GLUT4 to the plasma membrane in muscle cells. Ectopic expression of a constitutively activated RalA mutant indeed stimulated GLUT4 translocation, suggesting an important role of RalA also in muscle cells. GLUT4 translocation induced by constitutively activated mutation of Rac1 or more physiologically by upstream Rac1 regulators, such as phosphoinositide 3 kinase and the guanine nucleotide exchange factor FLJ00068, was abrogated when the expression of RalA was downregulated by RNA interference. The expression of constitutively activated Rac1, on the other hand, caused GTP loading and subcellular redistribution of RalA. Collectively, we propose a novel mechanism involving RalA for Rac1-mediated GLUT4 translocation in skeletal muscle cells.

  Nov. 2012, Cellular signalling, 24(11) (11), 2111 - 7, English, International magazine

  Scientific journal


• Effects of postmortem aging and cooking procedures on intramuscular fat and fatty acid composition in Japanese Black Wagyu beef.

  YAMANOUE MINORU, YANO KEIKO, HISANO EMI, UEDA SHUJI, IHARA IKKO, TOYODA KIYOHIKO

  The aim of this study was to analyze the changes in intramuscular fat of Japanese Black Wagyu beef after postmortem aging and cooking. Sample meats were taken from carcass cross-section between the sixth and seventh ribs of Japanese Black Wagyu cattle and stored at 4°C. At 7-, 14- and 28-days postmortem, total lipid was extracted with a mixture of chloroform and methanol accord

  National Organizing Committee for Technical Program, Nov. 2012, Proc. 15th AAAP Animal Science Congress, 2962 - 2968, English

  [Refereed]

  International conference proceedings


• Crucial role of the small GTPase Rac1 in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma

  Shuji Ueda, Sohei Kitazawa, Kota Ishida, Yuki Nishikawa, Megumi Matsui, Hikaru Matsumoto, Takuji Aoki, Shinsuke Nozaki, Tomoya Takeda, Yoshikazu Tamori, Atsu Aiba, C. Ronald Kahn, Tohru Kataoka, Takaya Satoh

  Jul. 2010, FASEB JOURNAL, 24(7) (7), 2254 - 2261, English

  [Refereed]

  Scientific journal


• Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1

  Hoshimi Kanemura, Takaya Satoh, Shymaa E. Bilasy, Shuji Ueda, Masanori Hirashima, Tohru Kataoka

  Oct. 2009, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 387(4) (4), 754 - 759, English

  [Refereed]

  Scientific journal


• Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10

  Takuji Aoki, Shuji Ueda, Tohru Kataoka, Takaya Satoh

  Jul. 2009, BMC CELL BIOLOGY, 10, English

  [Refereed]

  Scientific journal


• Dorsal telencephalon-specific RA-GEF-1 knockout mice develop heterotopic cortical mass and commissural fiber defect

  Shymaa E. Bilasy, Takaya Satoh, Shuji Ueda, Ping Wei, Hoshimi Kanemura, Atsu Aiba, Toshio Terashima, Tohru Kataoka

  May 2009, EUROPEAN JOURNAL OF NEUROSCIENCE, 29(10) (10), 1994 - 2008, English

  [Refereed]

  Scientific journal


• Activation of the small GTPase Rac1 by a specific guanine-nucleotide-exchange factor suffices to induce glucose uptake into skeletal-muscle cells

  Shuji Ueda, Tohru Kataoka, Takaya Satoh

  Nov. 2008, BIOLOGY OF THE CELL, 100(11) (11), 645 - 657, English

  Scientific journal


• Quantitative analysis of spatial regulation of low molecular weight GTPases and its medical application

  Shuji Ueda, Tohru Kataoka, Takaya Satoh

  2006, Progress on Post-Genome Technologies, 224 - 226, English

  [Refereed]

  International conference proceedings


• リューマチ様関節炎(RA)疾患遺伝子としてのDblプロトオンコジーンスプライシング変異体の分子的性質(Molecular characterization of Dbl proto-oncogene splicing variant as rheumatoid arthritis (RA) disease gene)

  Komai Koichiro, Arakawa Ayumi, Osawa Kayo, Gake Maiko, Ueda Shuji, Satoh Takaya, Kataoka Tohru, Shiozawa Shunichi

  (公社)日本生化学会, Aug. 2005, 生化学, 77(8) (8), 947 - 947, English


• Role of the Sec14-like domain of Dbl family exchange factors in the regulation of Rho family GTPases in different subcellular sites

  UEDA, Shuji, Kataoka Tohru, SATOH, Takaya

  Aug. 2004, Cellular Signalling, Vol. 16, No. 8, pp. 899-906, English

  [Refereed]

  Scientific journal


• The N-terminal sequence of paratropomyosin binding fragments from β-connectin

  Minoru Yamanoue, Shuji Ueda, Akiko Ohashi, Yumi Yoshimura, Shigemi Norioka

  Jan. 2003, Bioscience, Biotechnology and Biochemistry, 67(3) (3), 563 - 569, English

  [Refereed]

  Scientific journal


• Epidermal growth factor stimulation of the ACK1/Dbl pathway in a Cdc42 and Grb2-dependent manner

  Juran Kato-Stankiewicz, Shuji Ueda, Tohru Kataoka, Yoshito Kaziro, Takaya Satoh

  Academic Press Inc., 2001, Biochemical and Biophysical Research Communications, 284(2) (2), 470 - 477, English

  [Refereed]

  Scientific journal

• 第69 回国際食肉科学技術会議（ICoMST2023）に出席して

  上田 修司, 坂田 亮一, 江草 愛, 和賀 正洋, 若松 純一, 岩﨑 智仁, オークル ガムゼ, 長澤 麻央, 有原 圭三

  Nov. 2023, 食肉の科学, 第64巻(第2号) (第2号)

  Meeting report


• 畜産物の品質評価、付加価値の向上に向けたメタボロミクス技術の応用に関する研究

  上田 修司

  Corresponding, Nov. 2023, 六篠会報, Japanese

  [Invited]

  Introduction research institution


• 産官学連携による神戸ビーフの美味しさの研究取り組みの紹介

  北村 千晶, 上田, 修司

  Corresponding, Mar. 2023, 畜産兵庫・公益社団法人兵庫県畜産協会, (7) (7), Japanese

  [Refereed][Invited]

  Introduction commerce magazine


• 筋細胞の小胞体ストレス応答におけるHSPB5の結合分子の探索と機能解析

  西原萌華, 上田修司, 吉野健一, 吉野健一, 竹内敦子, 山之上稔, 白井康仁

  2020, 日本農芸化学会関西支部講演会講演要旨集, 512th


• 低親和性ビオチン結合タンパク質Tamavidinを用いた新規ペプチド捕捉型BioID法の開発

  吉野健一, 吉野健一, 西原萌華, 上田修司, 竹内敦子

  2019, 日本組織細胞化学会総会・学術集会講演プログラム・予稿集, 60th


• BioID法を用いたHSPB5の新規相互作用分子の探索

  西原萌華, 上田修司, 吉野健一, 吉野健一, 竹内敦子, 山之上稔, 白井康仁

  2019, 日本分子生物学会年会プログラム・要旨集(Web), 42nd


• ジアシルグリセロールキナーゼαの新規結合分子の探索

  木本裕子, 渡辺真以, 上田修司, 吉野健一, 山之上稔, 白井康仁

  2019, 日本農芸化学会関西支部講演会講演要旨集, 507th


• Tamavidin-REVを用いたタンパク質間相互作用の網羅的解析

  Ueda Shuji, Yoshino Kenichi, 山田聡一郎

  Jan. 2018, 和光純薬時報, 86(1) (1), 6 - 7, Japanese

  [Invited]

  Introduction other


• 黒毛和牛の魅力創出技術セミナーの開催報告

  UEDA SHUJI, YAMANOUE MINORU

  日本食肉研究会, Jun. 2017, 食肉の科学, 58(1) (1), 49 - 52, Japanese

  Meeting report


• リチウムによるDGKβKOマウスの記憶及び感情障害の改善には、PIP2の減少とPKCβの活性抑制が関与している

  沖本 航, 中井 寛子, 石坂 光絵, 中西 広樹, 森口 茂樹, 上田 修司, 山之上 稔, 福永 浩司, 佐々木 雄彦, 原 英彰, 白井 康仁

  日本脂質生化学会, May 2016, 脂質生化学研究, 58, 229 - 233, Japanese


• 多成分一斉解析技術による黒毛和種牛肉の食品成分の検討

  上田修司, 岩本英治, 正木達規, 谷元哲則, 渡代勝之, 白井康仁, 山之上稔

  2016, 日本畜産学会大会講演要旨, 121st


• Screening of novel diacylglycerol kinase alpha (DGK alpha) activator to develop drugs improving diabetic nephropathy

  Hayashi Daiki, Liu Ka, Ueda Shuji, Yamanoue Minoru, Sakane Fumio, Shirai Yasuhito

  Jul. 2015, JOURNAL OF PHARMACOLOGICAL SCIENCES, 128(3) (3), S102

  [Refereed]


• ジアシルグリセロールキナーゼαの218番目のチロシンリン酸化はドメイン間相互作用と高次構造を変化させる

  脇阪昌明, 岩下智秋, 濱田大三, 濱田大三, 祇園景子, 上田修司, 山之上稔, 鶴田宏樹, 白井康仁

  2015, 日本生化学会大会(Web), 88th


• チロシンリン酸化がジアシルグリセロールキナーゼαのドメイン間相互作用及び高次構造に与える影響

  脇阪昌明, 岩下智秋, 濱田大三, 濱田大三, 祇園景子, 上田修司, 山之上稔, 鶴田宏樹, 白井康仁

  2015, 日本農芸化学会関西支部講演会講演要旨集, 492nd


• mTOR is involved in both kinase activity-dependent and -independent pathways of DGK beta-regulated neurite and branch induction

  Takuya Kano, Hiroko Nakai, Akio Nakashima, Ushio Kikkawa, Naoaki Saito, Nobuyuki Takei, Shuji Ueda, Minoru Yamanoue, Yasuhito Shirai

  2014, JOURNAL OF PHARMACOLOGICAL SCIENCES, 124, 100P - 100P, English

  Summary international conference


• ジアシルグリセロールキナーゼβによる特徴的な神経突起伸にはcPKCも関与する

  中井 寛子, 加野 拓也, 上田 修司, 山之上 稔, 斎藤 尚亮, 白井 康仁

  28 May 2013, 脂質生化学研究, 55, 44 - 47, Japanese


• Involvement of mTOR in diacylglycerol kinase beta (DGK beta)-induced neurite blanching and spine formation

  H. Nakai, N. Takei, A. Nakashima, U. Kikkawa, N. Saito, S. Ueda, M. Yamanoue, Y. Shirai

  Sep. 2012, FEBS JOURNAL, 279, 383 - 384, English

  Summary international conference


• ジアシルグリセロールキナーゼβによる特徴的な神経突起伸にはmTORが関与する

  白井 康仁, 中井 寛子, 中西 広樹, 中嶋 昭雄, 吉川 潮, 上田 修司, 山之上 稔, 斎藤 尚亮

  28 May 2012, 脂質生化学研究, 54, 98 - 100, Japanese


• 食肉の熟成と筋漿中の筋漿中のコネクチン断片増加

  UEDA SHUJI, YAMANOUE MINORU

  日本食肉研究会, Jun. 2011, 食肉の科学, 52(1) (1), 120 - 123, Japanese

  [Invited]

  Meeting report


• Role of the guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis by the small GTPase Rac1*

  Katsuaki Ieguchi, Shuji Ueda, Tohru Kataoka, Takaya Satoh

  Aug. 2007, JOURNAL OF BIOLOGICAL CHEMISTRY, 282(32) (32), 23296 - 23305, English


• 熟成中の食肉軟化を誘起する筋原線維タンパク質パラトロポミオシンの局在と遊離について

  山之上 稔, 費 莎, 上田 修司

  日本食肉研究会, 30 Jun. 2000, 食肉の科学, 41(1) (1), 102 - 105, Japanese

• 黒毛和種牛肉を取り巻く環境。神戸ビーフをおいしくする魔法の成分!?

  畜産兵庫・兵庫県畜産協会, Feb. 2025


• 神戸ビーフの魅力を引き立てる！赤身肉と相性の良い薬味とは！?

  上田修司

  畜産兵庫・兵庫県畜産協会, Aug. 2024


• 次世代シーケンス技術を活用した黒毛和種の筋内脂肪形成と香りに関する研究

  上田修司

  関西畜産学会報・関西畜産学会, May 2024


• 黒毛和種牛肉の輸出課題に向けた科学的なデータの集積 ― 日越の新たなパートナーシップに向けた取り組み

  上田修司

  機関誌ベトナム・公益社団法人ベトナム協会, Mar. 2024


• 牛肉輸出による国内枝肉卸売価格および生産量の下支え効果の推定

  八木浩平, 岡本泰生, 上田修司

  食肉四季報, Mar. 2024


• 産官学連携による神戸ビーフの美味しさの研究取り組みの紹介

  上田修司

  畜産兵庫･兵庫県畜産協会, Feb. 2024


• 畜産物の品質評価、付加価値の向上に向けたメタボロミクス技術の応用に関する研究

  上田修司

  Single work, 食肉の科学 第64巻 第1号・日本食肉科学会, Jun. 2023


• 「総説」黒毛和種牛肉の輸出課題に向けた科学的なデータの集積

  上田 修司

  Single work, 機関誌ベトナム・公益社団法人ベトナム協会, Mar. 2023


• 畜産物の品質評価、付加価値の向上に向けたメタボロミクス技術の応用に関する研究

  上田修司

  六篠会報 ・神戸大学農学部同窓会, 2023


• 「総説」科学的なデータに基づく日本産の黒毛和種牛肉の特性 ― 日越企業のパートナーシップの促進に向けた取り組み紹介 ―

  上田 修司

  機関誌ベトナム・公益社団法人ベトナム協会, Mar. 2022


• 和牛の魅力の科学的な情報発信の重要性について

  上田修司

  Single work, 畜産コンサルタント・公益社団法人中央畜産会, Jul. 2021


• 「総説」黒毛和種牛肉の特徴的な脂質・香りの解析 ― ベトナム市場への動物性食品の輸出促進を目指して ―

  上田修司

  機関誌ベトナム・公益社団法人ベトナム協会, Mar. 2021


• 畜産物資源の有効活用向けたメタボロミクス技術の開発

  上田修司

  アグリバイオ ・北隆館, Nov. 2019


• 健康食品成分の探索に向けたBirA酵素標識法の応用

  UEDA SHUJI

  Single work, アグリバイオ (北隆館), Oct. 2018, Japanese

  Scholarly book


• Comprehensive analysis of protein-protein interaction using Tamavidin-REV

  UEDA SHUJI, YOSHINO KENICHI, YAMADA SOICHIRO

  Joint work, Wako Pure Chemical Time Report (Issue：Wako Pure Chemical Corporation), Apr. 2018, Japanese, 近年、生命現象を解明する糸口として、生体内のタンパク質間の相互作用を包括的に同定・定量するインタラクトーム解析が一つの潮流になっている。現在、タンパク質の相互作用の検出を容易にする方法として、BioID法、チラミド化合物法、アスコルビン酸ペルオキシダーゼ法などの酵素標識技術が実用化されている。本稿では、我々が実施しているBioID技術について紹介する。

  General book


• Study of salty-taste related peptides for the progress of low-salt animal food

  UEDA SHUJI, YAMANOUE MINORU

  Single work, The Salt Science Research Foundation, Jul. 2017, Japanese, 牛肉に含まれるジペプチドについて、液体クロマトグラフィー質量分析計による網羅的な解析の結果、332ピークを検出し、多変量解析より黒毛和種牛肉により多く含まれる複数のジペプチドを推定した。また、部位別の食味アンケート調査より、牛肉のラム芯 (臀筋) で塩味が最も強く認識されることが示された。動物性食品に伴う塩分摂取を配慮する上で、塩味成分と部位に関する有用な情報を得ることができた。

  Scholarly book


• 高齢者の健康維持機能に関わる動物性食品由来の新規機能性成分の探索に関する研究報告書

  UEDA SHUJI

  Single work, 全国食肉事業協同組合連合会, Mar. 2016, Japanese

  Report


• 「農」イノベーションひょうご 研究開発プロジェクト支援事業（先導的研究枠）報告書

  UEDA SHUJI

  Single work, 兵庫県, May 2015, Japanese

  Report


• 兵庫県COEプログラム推進事業 成長産業育成支援枠 「F/S 調査ステージ研究」

  UEDA SHUJI

  Single work, 兵庫県, Apr. 2015, Japanese

  Report

• Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef

  Shuji Ueda, Chiaki Kitamura, Mana Hosoda, Itsuko Fukuda, Yasuhito Shirai

  Boston Convention & Exhibition Center, Dec. 2023, English

  Poster presentation


• The extracellular environment in micro intramuscular fat accumulation formation in Japanese Black cattle beef

  Ueda Shuji, Kitamura Chiaki, Hosoda Mana, Yamanoue Minoru, Fukuda Itsuko, Shirai Yasuhito

  Padova congress, Aug. 2023, English

  [Invited]

  Nominated symposium


• Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef

  Ueda Shuji, Gotou Yunosuke, Takashima Yasuharu, Suzuki Takeshi Kitamura, Chiaki, Fukuda Itsuko, Shirai Yasuhito

  Padova congress, Aug. 2023, English

  Poster presentation


• Research on the application of metabolomics technology for quality evaluation and improvement of additional value of livestock products.

  Ueda Shuji

  Japan Society for Meat Science and Technology, Mar. 2023

  [Invited]

  Invited oral presentation


• Functional analysis of small heat shock protein HSPB1 in mechanotransduction in myofibroblast cells

  Kunimitsu Fukushima, Shuji Ueda, Itsuko Fukuda, Yasuhito Shirai

  第95回日本生化学会大会, Nov. 2022

  Poster presentation


• Elucidation of the suppression mechanism of endoplasmic reticulum stress by small molecular heat shock protein HSPB5.

  Yuuki Hioka, Shuji Ueda, Moeka Nishihara, Itsuko Fukuda, Yasuhito Shirai

  第95回日本生化学会大会, Nov. 2022

  Poster presentation


• Polo-like kinase 2 plays an essential role in cytoprotection in endoplasmic reticulum stress via phosphorylation of serine 19 in HSPB5

  Yuuki Hioka, Shuji Ueda, Moeka Nishihara, Ken-ichi Yoshino, Soichiro Yamada, Minoru Yamanoue, Yasuhito Shirai

  The American Society for Cell Biology Cell Bio 2021 Virtual-an Online ASCB|EMBO Meeting, Dec. 2021

  Poster presentation


• Phosphorylation of HSPB1 regulates actin-myosin contraction in muscle cells via binding to the essential light chain.

  Kunimitu Fukushima, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai, Shuji Ueda

  The American Society for Cell Biology Cell Bio 2021 Virtual-an Online ASCB|EMBO Meeting, Dec. 2021

  Poster presentation


• Small heat shock protein suppresses apoptosis caused by endoplasmic reticulum stress in L6 myocytes

  Yumeno Mitani, Shuji Ueda, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai

  The American Society for Cell Biology Cell Bio 2020 Virtual-an Online ASCB|EMBO Meeting, Dec. 2020

  Oral presentation


• DGKθ is involved in cell proliferation of mouse embryonic fibroblasts and affects fetal development.

  Kazuki Ito, Shuji Ueda, Nao Koizumi, Minoru Yamanoue, Yoshitaka Fujihara, Masahito Ikawa, Yasuhito Shirai

  The American Society for Cell Biology Cell Bio 2020 Virtual-an Online ASCB|EMBO Meeting, Dec. 2020

  Poster presentation


• Proteomic analysis of senescence-like morphology induced by DNA damage in NIH3T3 cells

  Aika Hara, Yoshino Ken-ichi, Atsuko Takeuchi, Minoru Yamanoue, Yasuhito Shirai, Shuji Ueda

  The American Society for Cell Biology Cell Bio 2020 Virtual-an Online ASCB|EMBO Meeting, Dec. 2020

  Poster presentation


• 黒毛和種の筋肉に生じる微小な脂肪細胞分布に関する研究

  UEDA SHUJI, 井上 亜希子, YAMANOUE MINORU

  第60回日本食肉研究会60周年記念大会, Mar. 2019, Japanese, 日本食肉研究会, 麻布大学, Domestic conference

  Poster presentation


• 鶏骨格筋パラトロポミオシン構成サブユニットによる合成アクトミオシンATPase活性阻害

  YOSHIDA HIROSHI, NISHIO YURIKO, UEDA SYUJI, YAMANOUE MINORU

  日本畜産学会第125回大会, Mar. 2019, Japanese, 麻布大学, Domestic conference

  Oral presentation


• Effects of adding FFAs on beef taste-traits analyzed by electronic taste sensing system and sensory evaluation

  Zhao Yanan, Di Yindi, Ueda Shuji, SHIRAI YASUHITO, HABARA MASAAKI, IKEZAKI HIDEKAZU, YAMANOUE MINORU

  日本畜産学会第125回大会, Mar. 2019, English, 麻布大学, Domestic conference

  Oral presentation


• 讃岐牛・オリーブ牛の 低需要部位の魅力を引き出す取り組み

  UEDA SHUJI

  平成30年度「オリーブ牛」セミナー, Feb. 2019, Japanese, 高松国際ホテル, Domestic conference

  Public discourse


• オリーブ牛をモデルに用いた 国産牛肉の低需要部位の 部位別科学分析と、その訴求に向けた罐詰の試作の評価

  UEDA SHUJI

  国産食肉等新需要創出緊急対策事業に関わるオリーブ牛の低需要部位の特性等セミナー, Feb. 2019, Japanese, 香川県社会福祉総合センター, Domestic conference

  Public discourse


• 試食アンケートから見るオリーブ牛のおいしさについて

  UEDA SHUJI

  平成30年度「オリーブ牛」セミナー, Jan. 2019, Japanese, Domestic conference

  Public discourse


• Comparative metabolomics of skeletal muscle of livestock using gas chromatography–mass spectrometry

  UEDA SHUJI, Iwamoto Eiji, Kato Yoshiki, Shinohara Masakazu, Shirai Yasuhito, Yamanoue Minoru

  The American Society for Cell Biology 2018 ASCB/IFCB Meeting, Dec. 2018, English, San Diego conventional center (California), International conference

  Poster presentation


• ノックアウトマウスを用いた皮膚の創傷治癒におけるDGKθの機能解析

  山口茉子, UEDA SHUJI, 小泉奈緒, 伊藤和樹, 伊川正人, 山之上稔, 白井康仁

  The 41nd Annual Meeting of the Molecular Biology Society of Japa, Nov. 2018, Japanese, パシフィコ橫浜, Domestic conference

  Poster presentation


• 平成30年度黒毛和牛の魅力創出技術構築事業の紹介

  UEDA SHUJI

  平成30年度第2 回 黒毛和牛の魅力創出技術セミナー, Sep. 2018, Japanese, 神戸大学瀧川記念学術交流会館, Domestic conference

  Public discourse


• 鶏骨格筋由来の組換えパラトロポミオシンサブユニットがアクチン–ミオシン間の相互作用に及ぼす影響

  YOSHIDA HIROSHI, NISHIO YURIKO, UEDA SYUJI, YAMANOUE MINORU

  第68回関西畜産学会香川大会, Sep. 2018, Japanese, 香川大学農学部, Domestic conference

  Oral presentation


• RhoA結合蛋白質として同定されたSTE20 like kinaseの 筋細胞の肥大化における役割

  Kushiya Akiho, UEDA SHUJI, Maeda Manami, Kato Yoshiki, Yoshino Ken-ichi, Takeuchi Atsuko, Yamanoue Minoru, Shirai Yasuhito

  The 91st Annual Meeting of the Japanese Biochemical Society, Sep. 2018, Japanese, 国立京都国際会館, Domestic conference

  Oral presentation


• Effects of intramuscular FFAs on beef taste-traits analyzed by electronic taste sensing system and sensory evaluation

  ZHAO YANAN, NAKADA YUSUKE, UEDA SYUJI, SHIRAI YASUHITO, ICHIMURA SAYAKA, YOSHIDA YUKA, HABARA MASAAKI, IKEZAKI HIDEKAZU, YAMANOUE MINORU

  The 64th ICoMST, Aug. 2018, English, Melbourne, Australia, International conference

  Poster presentation


• 平成30年度黒毛和牛の魅力創出技術構築事業の紹介

  UEDA SHUJI

  平成30年度第1 回 黒毛和牛の魅力創出技術セミナー, Jul. 2018, Japanese, 神戸大学瀧川記念学術交流会館, Domestic conference

  Keynote oral presentation


• 味覚センサ分析および官能評価による牛肉呈味性への筋内脂肪の影響

  趙 婭楠, 中田悠介, UEDA SHUJI, SHIRAI YASUHITO, 市村 さやか, 吉田 由香, 羽原正秋, 池崎秀和, YAMANOUE MINORU

  第59回日本食肉研究会大会, Mar. 2018, Japanese, 東京大学農学部, Domestic conference

  Oral presentation


• 熟成中の牛肉の筋内脂肪変化に影響される呈味性の味覚センサおよび官能評価による分析 (第2報)

  趙 婭楠, 中田悠介, UEDA SHUJI, SHIRAI YASUHITO, 市村 さやか, 吉田 由香, 羽原正秋, 池崎秀和, YAMANOUE MINORU

  日本畜産学会第124回大会, Mar. 2018, Japanese, 東京大学農学部, Domestic conference

  Oral presentation


• ジアシルグリセロールキナーゼgammaを作用点とする分子標的機能性食品の開発に向けた研究

  倉橋 弘平, 菊永 佐紀子, UEDA SHUJI, YAMANOUE MINORU, SHIRAI YASUHITO

  日本農芸化学会関西支部第501回講演会, Dec. 2017, Japanese, 神戸大学農学部, Domestic conference

  Oral presentation


• Investigation of RhoA binding proteins using BioID system

  Kushiya Akiho, Yamada Soichiro, Yoshino Ken-ichi, Kato Yoshiki, Takeuchi Atsuko, Sabourin Luc A, Cybulsky Andrey V, Yamanoue Minoru, Shirai Yasuhito, UEDA SHUJI

  The American Society for Cell Biology 2017 ASCB/IFCB Meeting, Dec. 2017, English, Philadelphia conventional center, Pennsylvania, International conference

  Poster presentation


• HSPB1 protein increases actomyosin ATPase activity of myofibrils fraction

  Yoshiki Kato, Kokaji Yoshito, Yoshino Ken-ichi, Takeuchi Atsuko, Yamanoue Minoru, Shirai Yasuhito, UEDA SHUJI

  The American Society for Cell Biology 2017 ASCB/IFCB Meeting, Dec. 2017, English, Philadelphia conventional center, Pennsylvania, International conference

  Poster presentation


• 緑茶カテキン EGCg のメチル化による糖尿病性腎症改善効果の増強

  HAYASHI Daiki, UEDA Shuji, YAMANOUE Minoru, ASHIDA Hitoshi, SHIRAI Yasuhito

  日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会（第49回講演会）, Sep. 2017, Japanese, Sakai, Domestic conference

  Oral presentation


• 熟成中の牛肉における筋内脂肪変化に影響される呈味性の味覚センサおよび官能評価による分析

  趙 婭楠, 中田悠介, UEDA SHUJI, SHIRAI YASUHITO, 羽原正秋, 池崎秀和, YAMANOUE MINORU

  日本畜産学会第123回大会, Sep. 2017, Japanese, 信州大学伊那キャンパス, Domestic conference

  Oral presentation


• 熟成および加熱牛肉の呈味物質変化と味覚センサによる呈味性分析

  中田悠介, 趙 婭楠, UEDA SHUJI, 鈴木 杏子, 岡 恵理子, 高岡 素子, 羽原正秋, 池崎秀和, YAMANOUE MINORU

  日本畜産学会第123回大会, Sep. 2017, Japanese, 信州大学伊那キャンパス, Domestic conference

  Oral presentation


• 質量分析計による黒毛和種牛肉の栄養成分のノンターゲット分析による検討

  UEDA SHUJI, 岩本英治, SHIRAI YASUHITO, YAMANOUE MINORU

  日本畜産学会第123回大会, Sep. 2017, Japanese, 信州大学伊那キャンパス, Domestic conference

  Oral presentation


• 動物性食品の適塩普及に向けた 塩味増強ペプチドの効果的な探索

  UEDA SHUJI

  ソルト・サイエンス研究財団「第29回助成研究発表会」, Jul. 2017, Japanese, ソルトサイエンス研究財団, 都市センターホテル, Domestic conference

  Oral presentation


• 多角的な視点から讃岐牛・オリーブ牛の魅力を引き出す 科学分析を主体とした取り組み

  UEDA SHUJI

  平成29年度讃岐牛・オリーブ牛振興会総会, May 2017, Japanese, 高松社会福祉総合センター, Domestic conference

  Public discourse


• 食肉の風味に寄与する香気成分分析法の確立

  岸本 徹, UEDA SHUJI, 岩本 英治

  日本畜産学会第122回大会, Mar. 2017, Japanese, 神戸大学鶴甲第1キャンパス, Domestic conference

  Oral presentation


• 熟成中の食肉軟化に寄与するパラトロポミオシンを構成するサブユニットの塩基配列解析

  NISIO YURIKO, UEDA SYUJI, MAEDA NAOYUKI, HATTORI AKIHITO, YAMANOUE MINORU

  日本畜産学会第122回大会, Mar. 2017, Japanese, 日本畜産学会, 神戸大学（兵庫県神戸市）, Domestic conference

  Oral presentation


• 種々の牛肉サンプルの味覚センサーおよび官能評価による呈味性分析

  ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SYUJI, HABARA MASAAKI, IKEZAKI HIDEKAZU, YAMANOUE MINORU

  日本畜産学会第122回大会, Mar. 2017, Japanese, 日本畜産学会, 神戸大学（兵庫県神戸市）, Domestic conference

  Oral presentation


• 黒毛和種牛肉の赤身部位の各種分析技術の検討

  UEDA SHUJI, 岩本 英治, 吉田 和利, SHINOHARA MASAKAZU, 中田 悠介, 高杉 瑠美, 岩本 英樹, 野村 郁代, 小川 隆文, SHIRAI YASUHITO, YAMANOUE MINORU

  日本畜産学会第122回大会, Mar. 2017, Japanese, 神戸大学鶴甲第1キャンパス, Domestic conference

  Oral presentation


• 黒毛和種牛肉の香成分における産地別特徴

  岩本 英治, 岸本 徹, UEDA SHUJI

  日本畜産学会第122回大会, Mar. 2017, Japanese, 神戸大学鶴甲第1キャンパス, Domestic conference

  Oral presentation


• 牛肉の美味しさとオリーブ牛の官能検査評価について

  UEDA SHUJI

  「オリーブ牛」ブランド化に係る事業成果報告会, Jan. 2017, Japanese, 平成２８年度地域コンソーシアム支援事業, ＪＲホテルクレメント高松, Domestic conference

  [Invited]

  Invited oral presentation


• 皮膚におけるジアシルグリセロールキナーゼγの機能解析

  菊永佐紀子, 横谷美由希, 藤原祥高, UEDA SYUJI, YAMANOUE MINORU, 高岡幸二, 伊川正人, SHIRAI YASUHITO

  日本農芸化学会関西支部例会 第497回講演会, Dec. 2016, Japanese, Domestic conference

  Oral presentation


• Identification of novel RhoA binding proteins by using BioID system

  櫛谷 晃帆, 加藤 良毅, Soichiro Yamada, YOSHINO KEN-ICHI, 竹内 敦子, YAMANOUE MINORU, SHIRAI YASUHITO, UEDA SHUJI

  The American Society for Cell Biology 2016 ASCB/IFCB Meeting, Dec. 2016, English, Moscone Center San Francisco, California, International conference

  Poster presentation


• Identification of novel HSPB1 binding proteins in skeletal muscle

  加藤 良毅, 小鍛治 泰斗, 櫛谷 晃帆, YOSHINO KEN-ICHI, 竹内 敦子, YAMANOUE MINORU, SHIRAI YASUHITO, UEDA SHUJI

  The American Society for Cell Biology 2016 ASCB/IFCB Meeting, Dec. 2016, English, Moscone Center San Francisco, California, International conference

  Poster presentation


• Identification of novel RhoA binding protein by usinBioID system

  櫛谷晃帆, UEDA SHUJI, 加藤良毅, YOSHINO KEN-ICHI, 竹内敦子, YAMANOUE MINORU, SHIRAI YASUHITO

  第39回日本分子生物学会年会, Dec. 2016, Japanese, パシフィコ横浜 展示ホール, Domestic conference

  Poster presentation


• 熟成による黒毛和種およびホルスタイン種牛肉汁中の呈味物質量の変化

  NAKADA YUSUKE, ZHAO YANAN, NISHIDA MASAHIRO, INOUE TOMOKA, UEDA SYUJI, OKA ERIKO, TAKAOAKA MOTOKO, YAMANOUE MINORU

  第66回関西畜産学会大会, Oct. 2016, Japanese, 関西畜産学会, 香川大学（香川県高松市）, Domestic conference

  Oral presentation


• 骨格筋におけるHSPB1結合蛋白質の探索と

  Kokaji Yoshito, Kato Yoshiki, Kushiya Akiho, YOSHINO KEN-ICHI, Takeuchi Atsuko, UEDA SHUJI, YAMANOUE MINORU, SHIRAI YASUHITO

  日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, 滋賀県立大学（滋賀県彦根市八坂町2500）, Domestic conference

  Oral presentation


• Effects of intramuscular FFAs on taste-traits of Japanese Black Wagyu beef

  ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SYUJI, IHARA IKKO, TOYODA KIYOHIKO, YAMANOUE MINORU

  62nd International Congress of Meat Science and Technology, Aug. 2016, English, Organizing Committee of 62nd ICoMST, Swissotel Le Concorde Bangkok (Bangkok, Thailand), International conference

  Oral presentation


• Difference between a myofibrillar protein paratropomyosin and tropomyosin based on their primary structure

  NISHIO YURIKO, USHIMURA YUICHI, UEDA SYUJI, MAEDA NAOYUKI, HATTORI AKIHITO, YAMANOUE MINORU

  17th AAAP Animal Science Congress, Aug. 2016, English, Japanese Society of Animal Science, Kyushu Sangyo University (Fukuoka, Japan), International conference

  Poster presentation


• Detection of the connectin/titin 20-kDa fragment increased in chicken sarcoplasm during postmortem aging

  NAKA AZUSA, MARUYAMA MASAYA, NAKAMA MINAMI, UEDA SYUJI, YAMANOUE MINORU

  17th AAAP Animal Science Congress, Aug. 2016, English, Japanese Society of Animal Science, Kyushu Sankyo University (Fukuoka, Japan), International conference

  Poster presentation


• Beef taste-traits assessed by an electronic taste sensing system

  ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SYUJI, HABARA MASAAKI, IKEZAKI HIDEKAZU, YAMANOUE MINORU

  17th AAAP Animal Science Congress, Aug. 2016, English, Japanese Society of Animal Science, Kyushu Sangyo University (Fukuoka, Japan), International conference

  Poster presentation


• 筋肉の増加減退に関わるRhoAの機能解析と相互作用分子の探索

  櫛谷 晃帆, UEDA SHUJI, 加藤 良毅, 桐村 悠佑, 石政 碧, YOSHINO KEN-ICHI, 竹内 敦子, YAMANOUE MINORU, SHIRAI YASUHITO

  第６３回日本生化学会近畿支部例会, May 2016, Japanese, 神戸薬科大学, Domestic conference

  Oral presentation


• 熟成中の牛肉におけるリパーゼ活性の変化

  ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SYUJI, IHARA IKKO, TOYODA KIYOHIKO, YAMANOUE MINORU

  日本畜産学会第121回大会, Mar. 2016, Japanese, 日本獣医生命科学大学（東京都）, Domestic conference

  Oral presentation


• 熟成による牛肉の脂肪および呈味性の変化

  YAMANOUE MINORU, ZHAO YANAN, NISHIDA MASAHIRO, UEDA SYUJI, IHARA IKKO, TOYODA KIYOHIKO

  第57回日本食肉研究会大会, Mar. 2016, Japanese, 日本獣医生命科学大学（東京都）, Domestic conference

  Oral presentation


• 骨格筋コネクチン断片の組換えタンパク質とモノクローナル抗体の結合について

  NAKA AZUSA, NAKAMA MINAMI, UEDA SHUJI, YAMANOUE MINORU

  若手フロンティア2015, Dec. 2015, Japanese, 神戸大学百年記念館, Domestic conference

  Poster presentation


• Insulin-like growth factor-1 induces activation and upregulating of via PI3K/Akt/mTOR pathway in muscular hypertrophy model of C2C12 cells

  Kushiya Akiho, UEDA SHUJI, Kirimura Yusuke, Katoh Yoshiki, Midori Ishimasa, Satoh Takaya, Yamanoue Minoru, Shirai Yasuhito

  American Society of Cell Biology, Dec. 2015, English, San Diego USA, International conference

  Poster presentation


• Force dependent biotinylation of myosin IIA by α-catenin tagged with a Promiscuous Biotin Ligase

  UEDA SHUJI, Alexandra M. Blee, Katherine G. Macway, Derrick J. Renner, Soichiro Yamada

  The American Society for Cell Biology, Dec. 2015, English, San Diego USA, International conference

  Poster presentation


• 和牛の新たな魅力の創出に向けた科学的な取り組み

  UEDA SHUJI

  全国会長会議, Nov. 2015, Japanese, 全国食肉事業協同組合連合会, ANAクラウンプラザ神戸, Domestic conference

  Public discourse


• Functional analysis of diacylglycerol kinase theta deficient mice

  Ueda Shuji, Koizumi Nao, Yamanoue Minoru, Ikawa Masahito, Shirai Yasuhito

  19th International Conference of FFC - 7th International Symposium of ASFFBC, Nov. 2015, English, Kobe University, International conference

  Poster presentation


• Diacylglycerol kinase as target of functional food to prevent and improve diabetic renal dysfunction

  HAYASHI Daiki, Yagi Keiko, UEDA Shuji, YAMANOUE Minoru, ASHIDA Hitoshi, EMOTO Noriaki, SAITO Naoaki, SHIRAI Yasuhito

  19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

  Poster presentation


• 神戸大学大学院農学研究科における食肉科学教育

  YAMANOUE MINORU, UEDA SYUJI

  食肉科学研究会主催懇話会, Sep. 2015, Japanese, 北海道大学アグリフードセンター, Domestic conference

  Public discourse


• 熟成中の食肉軟化に寄与するパラトロポミオシンは同程度の質量の2サブユニットで構成されている

  NISHIO YURIKO, YAMANOUE MINORU, USIMURA YUITI, UEDA SHUJI, MAEDA NAOYUKI, HATTORI AKIHIKO

  日本畜産学会第120回大会, Sep. 2015, Japanese, 酪農大学（江別市）, Domestic conference

  Oral presentation


• 種々の牛肉サンプルの味覚センサーによる客観的呈味性分析について

  ZHAO YANAN, NISHIDA MASAHIRO, NAKADA YUSUKE, UEDA SHUJI, HABARA MASAAKI, IKEZAKI HIDEKAZU, YAMANOUE MINORU

  平成27年度（第65回）関西畜産学会大会, Sep. 2015, Japanese, 愛媛大農学部（松山市）, Domestic conference

  Oral presentation


• 熟成中の牛肉筋漿における筋原線維Z線近傍コネクチン断片のELISA法による定量

  YAMANOUE MINORU, MARUYAMA MASAYA, UEDA SYUJI, SIRAI YASUHITO, SASAKI KANA

  第119回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮大学, Domestic conference

  Oral presentation


• Expression analysis of diacylglycerol kinase theta in mouse tissues by immunostaining and LacZ reporter assay

  UEDA SHUJI, Nao Koizumi, Minoru Yamanoue, Yasuhito Shirai

  The 88th Annual Meeting of The Japanese Pharmacological Society, Mar. 2015, Japanese, Nagoya Congress Corporation, Diacylglycerol kinase (DGK) regulates diacylglycerol signaling, which controls the variety of cell function. DGKθ belongs to the sole member of type V DGK. Several in vitro studies have suggested important role of DGKθ, but the physiological role of the enzyme is still unclear. In this study, to reveal the spatial expression of DGKθ, we performed the immunostaining on paraffin, Domestic conference

  Poster presentation


• Effects of storage and cooking on free fatty acid in Japanese Black Wagyu beef broth

  YAMANOUE MINORU, NISHIDA MASANORI, YAMATO SAYAKA, UEDA SYUJI, IHARA IKKO, TOYODA KIYOHIKO

  The 16th AAAP Animal Science Congress, Nov. 2014, English, Gadjah Mada University, Indonesia, International conference

  Poster presentation


• 筋細胞におけるHSPB1の結合分子の探索とその機能解析

  KOKAJI YASUTO, UEDA SHUJI, YOSHINO KEN-ICHI, YAMANOUE MINORU, SHIRAI YASUHITO

  第87回日本生化学会大会, Oct. 2014, Japanese, （公社）日本生化学会, 国立京都国際会館, Domestic conference

  Poster presentation


• 熟成および加熱による黒毛和種牛およびホルスタイン種牛肉汁脂質の脂肪酸組成変化

  NISHIDA MASAHIRO, UEDA SYUJI, IHARA IKKO, TOYODA KIYOHIKO, YAMANOUE MINORU

  第64回関西畜産学会大会, Sep. 2014, Japanese, 広島大学, Domestic conference

  Oral presentation


• ガレート型カテキンだけでなくビタミンEも67kDaラミニンレセプターを介してジアシルグリセロールキナーゼαを活性化する

  HAYASHI Daiki, ASHIDA Hitoshi, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 2014 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry in KANSAI, Sep. 2014, Japanese, Domestic conference

  Oral presentation


• Expression of diacylglycerol kinase theta during the organogenesis of mouse embryos

  UEDA Shuji, Tu-Sekine Becky, YAMANOUE Minoru, RABEN Daniel M, SHIRAI Yasuhito

  Lipid MAPS meeting, May 2014, English, LIPD MAPS, UCSD, International conference

  Poster presentation


• 鶏筋原線維のプロテアーゼ分解によるコネクチン20-kDa断片の生成

  YAMANOUE MINORU, SASAKI NODOKA, SASAKI KANA, UEDA SYUJI, SHIRAI YASUHITO

  第118回日本畜産学会大会, Mar. 2014, Japanese, つくば国際会議場, Domestic conference

  Oral presentation


• カテキン類によるジアシルグリセロールキナーゼα活性化機構の解明

  HAYASHI Daiki, ASHIDA Hitoshi, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 2014 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry, Mar. 2014, Japanese, Domestic conference

  Oral presentation


• カテキン類及びビタミンEは67kDaラミニンレセプターを介してジアシルグリセロールキナーゼαを活性化する (Chatechins and vitamin E activate diacylglycerol kinaseα through 67kDa laminin receptor)

  HAYASHI Daiki, ASHIDA Hitoshi, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 87th annual meeting of the Japanese biochemical society, 2014, Japanese, Domestic conference

  Poster presentation


• クロマン環構造に着目した新規ジアシルグリセロールキナーゼα活性化剤の探索

  林 大輝, ASHIDA HITOSHI, UEDA SHUJI, YAMANOUE MINORU, SAITO NAOAKI, SHIRAI YASUHITO

  第124回日本薬理学会近畿部会, Nov. 2013, Japanese, 日本薬理学会近畿部会, 京都ガーデンパレス, Domestic conference

  Poster presentation


• The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos

  UEDA Shuji, Tu-Sekine Becky, YAMANOUE Minoru, RABEN Daniel M, SHIRAI Yasuhito

  第86回日本生化学会大会, Sep. 2013, Japanese, 日本生化学会, パシフィコ横浜, Domestic conference

  Poster presentation


• 熟成および加熱による黒毛和種牛肉汁中のトリアシルグリセロールおよび遊離脂肪酸の変化

  YAMANOUE MINORU, 西田 昌弘, 大和 彩弥香, UEDA SHUJI, IHARA IKKO, TOYODA KIYOHIKO

  日本畜産学会第116回大会, Sep. 2013, Japanese, 日本畜産学会, 新潟大学, Domestic conference

  Oral presentation


• 熟成および加熱による黒毛和種牛肉汁の呈味性変化

  西田 昌弘, 大和 彩弥香, UEDA SHUJI, IHARA IKKO, TOYODA KIYOHIKO, YAMANOUE MINORU

  第63回関西畜産学会大会, Sep. 2013, Japanese, 関西畜産学会, 滋賀県立大学, Domestic conference

  Oral presentation


• ジアシルグリセロールキナーゼβによる特徴的な神経突起伸長にはcPKCも関与する

  NAKAI Hiroko, KANO Takuya, UEDA Shuji, YAMANOUE Minoru, SAITO Naoaki, SHIRAI Yasuhito

  第55回日本脂質生化学会大会, Jun. 2013, Japanese, 日本脂質生化学会, 宮城県松島町, Domestic conference

  Oral presentation


• The expression of diacylglycerol kinase theta during mouse embryonic development

  UEDA Shuji, Tu-Sekine Becky, YAMANOUE Minoru, RABEN Daniel M, SHIRAI Yasuhito

  The ASCB 2012 Annual meeting, Dec. 2012, English, American Society for Cell Biology, Moscone Center, USA, International conference

  Poster presentation


• DGKbetaによる神経突起伸張機構の解析

  KANO Takuya, UEDA Shuji, YAMANOUE Minoru, SAITO Naoaki, SHIRAI Yasuhito

  第85回日本生化学会大会, Dec. 2012, Japanese, 日本生化学会, 福岡国際会議場, Domestic conference

  Poster presentation


• Effects of Postmortem Aging and Cooking Procedures on Intramuscular Fat and Fatty Acid Composition in Japanese Black Wagyu Beef

  YAMANOUE Minoru, YANO Keiko, HISANO Emi, UEDA Shuji, IHARA Ikko, TOYODA Kiyohiko

  The 15th AAAP Animal Science Congress, Nov. 2012, English, Thai Society of Animal Science, Thammasat University, Thailand, International conference

  Poster presentation


• 熟成中の食肉軟化に寄与するパラトロポミオシンと調節タンパク質トロポミオシンのHPLCによる分離

  USHIMURA Yuuichi, OSHITA Emi, UEDA Shuji, YAMANOUE Minoru

  第62回関西畜産学会大会, Sep. 2012, Japanese, 関西畜産学会, 和歌山市, Domestic conference

  Oral presentation


• Molecular mechanism of DGKβ- mediated neurite blanching and spine formation

  NAKAI Hiroko, TAKEI Nobuyuki, NAKASHIMA Akio, KIKKAWA USHIO, SAITO Naoaki, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 35th Annual Meeting of the Japan Neuroscience Society, Sep. 2012, Japanese, Japan Neuroscience Society, Nagoya Congress Center, Domestic conference

  Poster presentation


• Involvement of mTOR in Diacylglycerol kinase β (DGKβ)-induced neurite branching and spine formation.

  NAKAI Hiroko, NAKASHIMA Akio, KIKKAWA USHIO, SAITO Naoaki, TAKEI Nobuyuki, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 22nd IUBMB & the 37th FEBS, Sep. 2012, English, IUBMB & FEBS, Sevilla, Spain, International conference

  Poster presentation


• Analysis of tissue distribution of diacylglycerol kinase theta

  UEDA Shuji, KIRIMURA Yuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 14th International Congress of Histochemistry and Cytochemistry, Aug. 2012, English, International Federation of Societies for Histochemistry and Cytochemisty, Kyoto International Conference Center, International conference

  Poster presentation


• ジアシルグリセロールキナーゼ_による特徴的な神経突起伸にはmTORが関与する

  SHIRAI Yasuhito, NAKAI Hiroko, NAKANISHI Hiroki, NAKASHIMA Akio, KIKKAWA Ushio, UEDA Shuji, YAMANOUE Minoru, SAITO Naoaki

  第54回日本脂質生化学会大会, Jun. 2012, Japanese, 日本脂質生化学会, 九州大学, Domestic conference

  Oral presentation


• 鶏肉の熟成に伴う筋漿中のコネクチン20-kDa断片増加量と筋原線維小片化の関連

  UEDA Shuji, OGAWA Yuriko, OHNISHI Kazumasa, YAMANOUE Minoru

  日本畜産学会第115回大会, Mar. 2012, Japanese, 日本畜産学会, 名古屋大学, Domestic conference

  Oral presentation


• ジアシルグリセロールキナーゼθの機能解析

  UEDA Shuji, KIRIMURA Yuji, YAMANOUE Minoru, SHIRAI Yasuhito

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都女子大学, Domestic conference

  Poster presentation


• 筋肉細胞におけるEGCgの糖代謝に及ぼす影響について

  上田 学UEDA Manabu, UEDA Syuji, SHIRAI Yasuhito, ASHIDA Hitoshi

  日本農芸化学会2012年度大会, 2012, Japanese, 京都, Domestic conference

  Oral presentation


• 鶏サルコメアA-I接合部におけるコネクチンとトロポミオシンの結合検証について

  大下 恵実, 福田 崇, UEDA SHUJI, YAMANOUE MINORU

  第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山大学, Domestic conference

  Oral presentation


• Changes in free fatty acids of Wagyu beef during post-mortem aging and cooking, and its palatability

  YAMANOUE Minoru, YANO Keiko, HISANO Emi, UEDA Shuji, IHARA Ikko, TOYODA Kiyohiko

  The 9th Pangborn Sensory Science Symposium, Sep. 2011, English, ELSEVIER, Toronto, Canada, International conference

  Poster presentation


• 熟成および加熱による牛筋内脂肪の遊離脂肪酸量と脂肪酸組成の変化

  矢野 恵子, 久野 絵美, IHARA IKKO, TOYODA KIYOHIKO, UEDA SHUJI, YAMANOUE MINORU

  日本畜産学会第114回大会, Aug. 2011, Japanese, 日本畜産学会, 北里大学, Domestic conference

  Poster presentation


• Characterization of antibodies against the connectin/titin 20-kDa fragment increased in chicken sarcoplasm during postmortem aging

  YAMANOUE Minoru, UEDA Shuji, MATSUNAGA Kenichi, OHNISHI Kazumasa, SHIOYAMA Natsuko

  The 57th International Congress of Meat Science and Technology, Aug. 2011, English, Association of the Belgian Meat Products Industry, Ghent, Belgium, International conference

  Poster presentation


• 熟成に伴う牛肉脂肪中の遊離脂肪酸量および組成の変化

  矢野 恵子, 久野 絵美, UEDA SHUJI, IHARA IKKO, TOYODA KIYOHIKO, YAMANOUE MINORU

  第60回関西畜産学会大会, Sep. 2010, Japanese, 関西畜産学会, 京都大学, Domestic conference

  Oral presentation


• Expression and antibody production of connectin fragment increased in sarcoplasm during postmortem aging of chicken muscle

  YAMANOUE Minoru, KUBO Takashi, MATSUNAGA Kenichi, UEDA Shuji, TAKAGI Michihiro

  The 56th International Congress of Meat Science and Technology, Aug. 2010, English, Korean Society for Food Science and Animal Resources/ National Society of Animal Science, Jeju, Korea, International conference

  Poster presentation


• 熟成に伴う豚肉筋漿中のタンパク質変化

  施 恭平, 橋本 真季, 塩山 奈都子, UEDA SHUJI, YAMANOUE MINORU

  日本畜産学会第112回大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

  Oral presentation


• 鶏サルコメアA-I接合部に及ぶコネクチンI帯末端領域構成ドメインの微生物発現

  福田 崇, 大下 恵実, UEDA SHUJI, YAMANOUE MINORU

  日本畜産学会第112回大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

  Oral presentation


• Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10

  Ueda Shuji, Kataoka Tohru, Satoh Takaya

  第32回日本分子生物学会年会, Dec. 2009, Japanese, 日本分子生物学会, 横浜, Domestic conference

  Poster presentation


• Impaired vascular network formation in mice lacking the Rap1 guanine nucleotide exchange factor RA-GEF-1

  Satoh Takaya, Ueda Shuji, EL-SHAWADFY, MOHAMED BILASY SHYMAA, Hirashima Masanori, Kataoka Tohru

  第82回日本生化学会大会, Oct. 2009, Japanese, 日本生化学会, 神戸, Domestic conference

  Poster presentation


• Dorsal telencephalon-specific RA-GEF-1 knockout mice develop heterotopic cortical mass and commissural fiber defect

  Terashima Toshio, EL-SHAWADFY, MOHAMED BILASY SHYMAA, Satoh Takaya, Ueda Shuji, Aiba Atsu, Kataoka Tohru

  第32回 日本神経科学大会, Sep. 2009, Japanese, 日本神経科学会, 名古屋, Domestic conference

  Poster presentation


• Dorsal telencephalon-specific RA-GEF-1 knockout mice develop heterotopic

  Terashima Toshio, EL-SHAWADFY, MOHAMED BILASY SHYMAA, Satoh Takaya, Ueda Shuji, Aiba Atsu, Kataoka Tohru

  第32回日本神経科学大会, Sep. 2009, Japanese, 日本神経科学学会, 名古屋, Domestic conference

  Poster presentation


• 鶏筋原線維Z線近傍でコネクチンに結合するモノクローナル抗体のエピトープ決定

  塩山 奈都子, UEDA SHUJI, YAMANOUE MINORU

  日本畜産学会第111回大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

  Oral presentation


• 豚肉の熟成に伴う筋原線維Z線近傍コネクチンの変化

  施 恭平, 橋本 真季, UEDA SHUJI, YAMANOUE MINORU

  第59回関西畜産学会大会, Aug. 2009, Japanese, 関西畜産学会, 鳥取大学, Domestic conference

  Oral presentation


• Role of the small GTPase Rac1 in insulin-dependent glucose uptake in skeletal muscle cells.

  Ueda Shuji, Tamori Yoshikazu, Aiba Atsu, Kataoka Tohru, Satoh Takaya

  神戸大学グローバルCOEプログラム「統合的膜生物学の国際教育研究拠点」 第3回ワークショップ, Jul. 2009, English, 神戸大学/グローバルCOE, 淡路, International conference

  Poster presentation


• Impaired vascular network formation in mice lacking the Rap1 guanine nucleotide exchange factor RA-GEF-1.

  Satoh Takaya, EL-SHAWADFY, MOHAMED BILASY SHYMAA, Ueda Shuji, Hirashima Masanori, Kataoka Tohru

  神戸大学グローバルCOEプログラム「統合的膜生物学の国際教育研究拠点」 第3回ワークショップ, Jul. 2009, English, 神戸大学/グローバルCOE, 淡路, International conference

  Poster presentation


• Dorsal telencephalon-specific RA-GEF-1 knockout mice develop heterotopic cortical mass and commissural fiber defect.

  EL-SHAWADFY, MOHAMED BILASY SHYMAA, Satoh Takaya, Ueda Shuji, Aiba Atsu, Terashima Toshio, Kataoka Tohru

  神戸大学グローバルCOEプログラム「統合的膜生物学の国際教育研究拠点」 第3回ワークショップ, Jul. 2009, English, 神戸大学/グローバルCOE, 淡路, International conference

  Poster presentation


• 鶏筋原線維Z線近傍コネクチン領域に対するモノクローナル抗体作製

  塩山 奈都子, UEDA SHUJI, YAMANOUE MINORU

  日本畜産学会第110回大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conference

  Oral presentation


• 骨格筋でのインスリン依存性糖取り込みにおけるRac1の機能解析

  Ueda Shuji, Kataoka Tohru, Satoh Takaya

  BMB2008（第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会）, Dec. 2008, Japanese, 日本分子生物学会/日本生化学会, 神戸, Domestic conference

  Poster presentation


• The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for mouse brain development.

  EL-SHAWADFY, MOHAMED BILASY SHYMAA, Satoh Takaya, Ueda Shuji, Aiba Atsu, Terashima Toshio, Kataoka Tohru

  BMB2008（第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会）, Dec. 2008, English, 日本分子生物学会/日本生化学会, 神戸, Domestic conference

  Others


• Dblファミリーグアニンヌクレオチド交換因子KIAA0294によるGTP結合蛋白質RhoAを介する中心体複製の制御．

  Ueda Shuji, Kataoka Tohru, Satoh Takaya

  BMB2008（第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会）, Dec. 2008, Japanese, 日本分子生物学会/日本生化学会, 神戸, Domestic conference

  Poster presentation


• Functional analysis of Rap1 guanine nucleotide exchange factors RA-GEF-1 and RA-GEF-2

  Ueda Shuji, Yoshikawa Yoko, Satoh Takaya

  第3回グローバルCOE研究討論会 兼グローバルCOE第2回ワークショップ, Jul. 2008, Japanese, グローバルCOE, 淡路, Domestic conference

  Poster presentation


• 筋細胞のインスリンシグナル伝達におけるRac1の機能解析

  Ueda Shuji, Aiba Atsu, Kataoka Tohru, Satoh Takaya

  第80回日本生化学会大会・第30回日本分子生物学会年会合同大会, Dec. 2007, Japanese, 日本生化学会,日本分子生物学会, 横浜, Domestic conference

  Oral presentation


• Role of the Rho family GTP-binding protein Rac1 and its guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis

  Ueda Shuji, KATAOKA TOHRU, Satoh Takaya

  46th Annual Meeting of the American Society for Cell Biology, Dec. 2006, English, Annual Meeting of the American Society for Cell Biology, サンディエゴ, International conference

  Poster presentation


• A novel method to detect the activation of small GTPases in cancer cells

  Shuji Ueda, Tohru Kataoka, Takaya Satoh

  The 65th Annual Meeting of the Japanese Cancer Association, Sep. 2006, Japanese, Yokohama, Domestic conference

  Poster presentation


• Analysis of the activation of small GTPases in cancer cells

  Ueda Shuji, KATAOKA TOHRU, Satoh Takaya

  第65回日本癌学会学術総会, Sep. 2006, Japanese, 日本癌学会, 横浜, Domestic conference

  Poster presentation


• Quantitative analysis of spatial regulation of low molecular weight GTPases and its medical application

  Ueda Shuji, KATAOKA TOHRU, Satoh Takaya

  The 4th International Forum on Post-Genome Technologies, Sep. 2006, English, Nanjing University／Southeast University／Zhejiang University／Jiangsu Science and Technology Association, Hangzhou, International conference

  Oral presentation


• Quantitative analysis of spatial regulation of low molecular weight GTPases and its medical applicatio

  Shuji Ueda, Takaya Satoh

  4th International Forum on Post-genome Technology, Sep. 2006, English, China Koushu, International conference

  Oral presentation


• Role of the Rho family GTP-binding protein Rac1 in clathrin-mediated receptor endocytosis

  Ueda Shuji, KATAOKA TOHRU, Satoh Takaya

  神戸大学21世紀COEプログラム「蛋白質のシグナル伝達機能」 平成18年度第1回研究発表会・若手ポスター発表会, Aug. 2006, Japanese, 神戸大学21世紀COEプログラム「蛋白質のシグナル伝達機能」, 神戸, Domestic conference

  Poster presentation


• Role of the Rho family GTP-binding protein Rac1 and the guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis

  Katsuaki ieguchi, Shuji Ueda, Tohru Kataoka, Takaya Satoh

  20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006, English, kyoto, Domestic conference

  Poster presentation


• Critical role of the Rho family GTP-binding protein Rac1 in insulin-stimulated GLUT4 translocation in skeletal muscle cells

  Shuji Ueda, Tohru Kataoka, Takaya Satoh

  20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006, English, Kyoto, International conference

  Poster presentation


• Critical role of the Rho family GTP-binding protein Rac1 in insulin-stimulated GLUT4 translocation in skeletal muscle cells

  Ueda Shuji, KATAOKA TOHRU, Satoh Takaya

  20th IUBMB Inetrnational Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress／第79回日本生化学会大会／第29回日本分子生物学会年会, Jun. 2006, English, The International Union of Biochemistry and Molecular Biology／The The Federation of Asian and Oceanian Biochemists and Molecular Biologists ／日本生化学会／日本分子生物学会, 京都, International conference

  Poster presentation


• ビネキシンノックダウンによる線維芽肉腫細胞HT１０８０の伸展能の抑制

  山下 依子, UEDA, Shuji, SATOH, Takaya

  日本農芸化学会2006年度大会, Mar. 2006, Japanese, 日本農芸化学会, 京都, Domestic conference

  Oral presentation


• Role of the Rho family GTP-binding protein Rac1 and its guanine nucleotide exchange factor Ost in negative regulation of receptor endocytosis

  家口 勝昭, UEDA, Shuji, KATAOKA, Toru

  International Symposium of Kobe University 21 century COE Program on Siganl Transduction In Memory of Prof. Yasutomi Nishizuka, Feb. 2006, English, 神戸大学21世紀COEプログラム, 神戸, International conference

  Poster presentation


• Critical role of the Rho family GTP-binding protein Rac1 in insulin-stimulated GLUT4 translocation in skeletal muscle cells

  UEDA, Shuji, KATAOKA, Toru, SATOH, Takaya

  International Symposium of Kobe University 21 century COE Program on Siganl Transduction In Memory of Prof. Yasutomi Nishizuka, Feb. 2006, English, 神戸大学21世紀COEプログラム, 神戸, International conference

  Poster presentation


• Regulation of Rho family GTP-binding proteins involved in insulin signaling in skeletal muscle.

  UEDA, Shuji, KATAOKA, Toru, SATOH, Takaya

  第28回日本分子生物学会年会, Dec. 2005, Japanese, 日本分子生物学会, 福岡, Domestic conference

  Poster presentation


• Function of the Dbl family GEF Ost-III in endocytosis of the transferring receptor

  家口 勝昭, UEDA, Shuji, KATAOKA, Toru

  第28回日本分子生物学会年会, Dec. 2005, Japanese, 日本分子生物学会, 福岡, Domestic conference

  Poster presentation


• Regulation of Rho family GTP-binding proteins involved in insulin signaling in skeletal muscle.

  UEDA, Shuji, KATAOKA, Toru, SATOH, Takaya

  第52回日本生化学会近畿支部例会, May 2005, Japanese, 日本生化学会, 神戸, Domestic conference

  Oral presentation


• Function of the Dbl family GEF Dbs in endocytosis of the transferring receptor

  家口 勝昭, UEDA, Shuji, KATAOKA, Toru

  第52回日本生化学会近畿支部例会, May 2005, Japanese, 日本生化学会, 神戸, Domestic conference

  Oral presentation


• Regulation of Rho family GTP-binding proteins involved in insulin signaling in skeletal muscle.

  UEDA, Shuji, KATAOKA, Toru, SATOH, Takaya

  第27回日本分子生物学会年会, Dec. 2004, Japanese, 日本分子生物学会, 神戸, Domestic conference

  Poster presentation


• Function analysis of the guanine nucleotide exchange factor Dbs

  家口 勝昭, UEDA, Shuji, KATAOKA, Toru

  第27回日本分子生物学会年会, Dec. 2004, Japanese, 日本分子生物学会, 神戸, Domestic conference

  Poster presentation


• Function of the SEC14-like domain of RhoGEF

  UEDA, Shuji, KATAOKA, Toru, SATOH, Takaya

  第76回 日本生化学会大会, Oct. 2003, Japanese, 日本生化学会, 横浜, Domestic conference

  Oral presentation

• The Japanese Society for Food Science and Technology

  2024 - Present


• Society of Beef Cattle Science

  2023 - Present


• 日本食品微生物学会

  Apr. 2025


• Kansai Animal Science Society


• The Japanese Society of Meat Science and Technology


• 米国細胞生物学会


• 日本畜産学会


• 日本生化学会


• 日本農芸化学会

• 筋細胞に生じる高齢者特有のプロテオスタシスの低下を軽減する分子機序の解明

  上田 修司, 吉野 健一

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, Apr. 2023 - Mar. 2026


• PL標識法による筋線維芽細胞の力伝達に関わる新機能探索と筋力低下予防への応用

  上田 修司

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, Apr. 2020 - Mar. 2023


• 筋萎縮予防に有益な健康食品成分の探索に向けたBirA酵素標識法の応用

  上田 修司

  学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• Effects of intramuscular free fatty acids on the response of taste buds and taste-traits of marbled beef

  Yamanoue Minoru, Ueda Syuji, Takaoka Motoko, Habara Masaaki, Ikezaki Hidekazu

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2016 - Mar. 2019

  Relationship between intramuscular fat and taste-traits of Japanese Black Wagyu beef which is well known as “marbling” and delicious beef was clarified in terms of the amounts of intramuscular free fatty acid (FFA). The amounts of FFA in beef broth prepared from Japanese Black Wagyu beef stored at 4℃ increased during postmortem aging, but not so in Holstein beef broth. As the results of estimation of taste-traits of broth sample by an electronic taste sensing system, some taste elements like acid bitterness showed high values and increased with postmortem time, indicating that increased FFA was possible to affect taste-traits of broth sample of Japanese Black Wagyu beef. Carrying out of Ca2+-imaging method are successively challenged in order to detect whether FFAs stimulate to taste cells prepared from mouse taste bud, and thereby possibly influence taste-traits of beef broth.


• BirA酵素標識法による運動時のHSPBsの標的分子の網羅的解析とその応用

  上田 修司

  学術研究助成基金助成金／基盤研究(C), Apr. 2014 - Mar. 2017, Principal investigator

  Competitive research funding


• Study of Rho family small G proteins in the increase of muscle size

  UEDA Shuji

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Kobe University, Apr. 2011 - Mar. 2013, Principal investigator

  Rho family GTPase RhoA has been known to be involved in the muscular development and reconstruction. However, the role of RhoA in IGF-1-induced muscular hypertrophy remain to be revealed. To investigate the regulatory mechanism of RhoA activation, we used muscular hypertrophy model of C2C12 cells. After IGF-I treatment, protein level of RhoA was upregulated, and activated RhoA was continuously increased in hypertrophic myotubes.RhoA dominant-negative mutant impaired prominent hypertrophy, and inhibited IGF-1-depending p38 MAPK activation and M-cadherin-shift, which are required for muscular differentiation.These results indicate that RhoA involves in multiple steps of IGF-I-induced muscular hypertrophy. To identify the key molecule in this mechanism, we designed RhoA tagged with promiscuous biotin ligase BirA and isolated several interesting RhoA binding candidate. This study clarified one end of the activation mechanism of RhoA related to muscular hypertrophy by IGF-1.

  Competitive research funding


• Expression, purification and function of recombinant connectin domains involved in meat tenderization during postmortem aging.

  YAMANOUE Minoru, UEDA Syuji

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2009 - 2011

  A myofibrillar protein, paratropomyosin weakens the rigor linkages between actin and myosin, and contributes to meat tenderization. Paratropomyosin was found at the A-I junction of myofibrils in living muscle and in muscle immediately postmortem. In chicken breast muscle paratropomyosin binds to connectin when examining binding of paratropomyosin at the A-I junction. In the present study, we produced all recombinant domains that constituted the connectin fragment at the junction and examined binding of paratropomyosin to each domain on PVDF membrane in order to clarify more precise binding site in connectin molecule. On the other hand, polyclonal and monoclonal antibodies produced against the connectin 20-kDa fragment, which was located in The Z-disk region and increased in chicken sarcoplasm with postmortem aging, were characterized in order to utilize for estimating meat aging.


• 活性型低分子量ＧＴＰａｓｅの細胞内局在の網羅的定量解析技術の開発とその応用

  佐藤 孝哉

  科学研究費補助金／特定領域研究, 2009

  Competitive research funding


• 低分子量ＧＴＰａｓｅの活性化動態の定量解析技術の開発と医学への応用

  上田 修司

  日本学術振興会, 科学研究費補助金／特定領域研究, 特定領域研究, 神戸大学, 2006 - 2006, Principal investigator

  低分子量GTPaseであるRasファミリーとRhoファミリーは、細胞内シグナル伝達のON/OFFを制御する分子スイッチとして機能しており、これらの活性調節異常は癌の発症に深く関与している。我々は、活性型低分子量GTPaseとエフェクター分子の特異的な結合特性に基づき、様々なエフェクター分子由来のポリペプチドプローブを作製し、低分子量GTPaseの活性化を免疫染色の要領で検出する方法を開発した。18年度は、Rasに対する新規プローブの開発を目指して、候補に挙がったエフェクター分子からプローブを作製し、低分子量GTPaseに対する特異性の検討を行った。また、プローブの検出感度の向上を目指して、エフェクター分子の結合ドメインを複数個繋ぐ事により結合力の強化を図った。活性の定量化法としては、データの客観性を高める手段として、プローブの検出を酵素免疫染色法(ELISA)で行う比色定量法を開発した。また、共焦点顕微鏡で撮影した蛍光免疫染色画像の蛍光強度をデジタル解析する事で、細胞内の低分子量GTPaseの活性の強さを3次元的に視覚化する事が可能になった。今回作製したプローブを用いて、癌培養細胞を検討したところ、Rasの恒常的活性型変異を有する癌細胞(SW480、PANC-1)では内在性Rasの活性が強く検出された。この結果は、従来法である細胞可溶化液を用いて行うプルダウンアッセイの結果と一致していた。細胞機能の多くは、複数の低分子量GTPaseの複雑なクロストークによって制御を受けている事から、さらに低分子量GTPase毎に異なる蛍光波長で検出する事ができるプローブを作製した。低分子量GTPaseの活性型変異体を発現する細胞で検討したところ、これらのプローブを用いれば二つの低分子量GTPaseの活性を同一細胞上で同時に検出し、比較する事ができる事がわかった。

  Competitive research funding


• インスリンシグナル伝達における低分子量ＧＴＰ結合蛋白質の機能解析

  上田 修司

  日本学術振興会, 科学研究費補助金／若手研究(B), 若手研究(B), 神戸大学, 2005 - 2006, Principal investigator

  インスリンによる血糖値降下作用は,糖輸送担体(GLUT4)の細胞膜への移行促進によって起こる。我々は,骨格筋のGLUT4の細胞膜移行の制御にアクチン細胞骨格を調節するRhoファミリー低分子量GTPaseが関与していると仮説を立て、検討している。18年度は、GLUT4にタグ配列を挿入したGFP-GLUT4-Mycを安定発現する分化効率が非常に高いL6細胞株を新に作製した。これを用いて、17年度に明らかにしたインスリン刺激で強く活性化されるRhoファミリーのRac1の機能解析を行った。筋管に分化誘導後のL6細胞にRac1の恒常的活性型変異体を、アデノウィルス発現システムを用いて過剰発現させ検討したところ、活性型Rac1はインスリン刺激と同様にアクチン細胞骨格の再構成を伴うGLUT4の細胞膜移行を強く促進した。RNAi干渉法によるRac1のノックダウンによって、L6細胞のインスリン刺激依存的なGLUT4の細胞膜移行が抑制される事から、我々はこれらの結果を個体レベルで更に検証するため、Cre/loxPシステムを用いてRac1の骨格筋特異的なノックアウトマウスを作製した。筋肉クレアチンキナーゼのプロモーター下流でCreを発現するトランスジェニックマウス(MCK-Cre)とRac1のfloxマウスを掛け合わせて、Rac1(flox/flox : MCK-Cre)マウスを作製し、後肢筋を調べたところ、タンパク質レベルでRac1の発現が著しく減少している事を確認した。

  Competitive research funding


• ＧＴＰ結合型低分子量ＧＴＰａｓｅの細胞内局在の網羅的解析

  佐藤 孝哉

  科学研究費補助金／特定領域研究, 2006

  Competitive research funding


• Ｒａｓ、ＲｈｏファミリーＧＴＰａｓｅの細胞内部位特異的な活性化の解析とその応用

  佐藤 孝哉

  科学研究費補助金／基盤研究(B), 2005

  Competitive research funding


• ＧＴＰ結合型低分子量ＧＴＰａｓｅの細胞内局在の網羅的解析

  佐藤 孝哉

  科学研究費補助金／特定領域研究, 2005

  Competitive research funding