Research activity information

• Immunohistochemical Evaluation of Tumor Characteristic Retention in Triple-negative Breast Cancer PDOX Models.

  Shotaro Inoue, Mayuko Miki, Sachiko Mizumoto, Mayuko Yamamoto, Motoi Baba, Hirokazu Tanino, Tomonari Kunihisa, Sachiko Inubushi

  BACKGROUND/AIM: Patient-derived xenograft (PDX) and Patient-derived orthotopic xenograft (PDOX) models are considered to recapitulate the heterogeneity and biological characteristics of original tumors more faithfully than conventional models. This study aimed to evaluate the extent to which specific tumor markers are retained in PDOX models of triple-negative breast cancer (TNBC), in comparison with their corresponding patient tumors. MATERIALS AND METHODS: PDOX models were established by orthotopically implanting tumor tissues from 15 TNBC patients into the mammary fat pads of immunodeficient mice. Immunohistochemical analyses were performed for CK5/6, HER2, EGFR, androgen receptor (AR), and Trop-2 in both the patient tumors and their corresponding PDOX tissues to evaluate the concordance of molecular marker expression. RESULTS: Trop-2 expression was consistently preserved across all tumor PDOX models. EGFR expression was preserved. In contrast, AR expression was observed in two of the original tumors, but it was completely lost in the corresponding PDOX models. CK5/6 and HER2 showed variable preservation. CONCLUSION: TNBC PDOX models are promising tools for preclinical drug evaluation; however, variability in the retention of molecular targets, such as AR, CK5/6, and HER2, emphasizes the importance of verifying marker expression prior to functional analysis. Tumor heterogeneity and clonal selection during engraftment may contribute to these discrepancies.

  Oct. 2025, Anticancer research, 45(10) (10), 4169 - 4180, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• PARP阻害剤耐性乳癌に対するメチオニン制限の新たな可能性

  三木 万由子, 犬伏 祥子, 岡島 美和, 北川 遥香, 井上 翔太郎, 山本 真由子, 明神 真由, 松尾 容子, 國久 智成

  (一社)日本乳癌学会, Jul. 2025, 日本乳癌学会総会プログラム抄録集, 33回, 427 - 427, Japanese


• TNBC-PDXモデルにおける患者腫瘍特性の保持についての検討

  井上 翔太郎, 犬伏 祥子, 岡島 美和, 北川 遥香, 明神 真由, 三木 万由子, 山本 真由子, 松尾 容子, 国久 智成

  (一社)日本乳癌学会, Jul. 2025, 日本乳癌学会総会プログラム抄録集, 33回, 428 - 428, Japanese


• 乳癌患者の術前後における血清中のCD9、CD63、HER2発現細胞外小胞の比較検討

  犬伏 祥子, 國久 智成, 国安 真理奈, 井上 翔太郎, 山本 真由子, 山下 祐司, 三木 万由子, 水本 紗千子, 馬場 基, 谷野 裕一

  (一社)日本乳癌学会, Jul. 2025, 日本乳癌学会総会プログラム抄録集, 33回, 531 - 531, Japanese


• 当院における神経線維腫症1型に対する乳癌サーベイランス

  國久 智成, 三木 万由子, 明神 真由, 松尾 容子, 山本 真由子, 井上 翔太郎, 犬伏 祥子

  (一社)日本乳癌学会, Jul. 2025, 日本乳癌学会総会プログラム抄録集, 33回, 548 - 548, Japanese


• 当院で術後薬物療法としてオラパリブを使用した症例の検討

  明神 真由, 國久 智成, 三木 万由子, 松尾 容子, 山本 真由子, 井上 翔太郎, 北川 遥香, 岡島 美和, 犬伏 祥子

  (一社)日本乳癌学会, Jul. 2025, 日本乳癌学会総会プログラム抄録集, 33回, 610 - 610, Japanese


• 乳房粘液腫によりCarney複合と診断された一例

  山本 真由子, 井上 翔太郎, 犬伏 祥子, 松尾 容子, 明神 真由, 三木 万由子, 國久 智成

  (一社)日本乳癌学会, Jul. 2025, 日本乳癌学会総会プログラム抄録集, 33回, 833 - 833, Japanese


• Association between preoperative grip strength and postoperative upper extremity impairments in patients with breast cancer: a retrospective cohort study

  Mayu Mizuta, Maho Okumura, Junichiro Inoue, Yuya Ueda, Shin Kondo, Mayuko Miki, Tomonari Kunihisa, Rei Ono, Yoshitada Sakai, Toshihiro Akisue

  Abstract Background Upper extremity impairments in patients with breast cancer persist after curative surgery. Although postoperative factors associated with upper extremity impairments have been reported, modifiable factors affecting these impairments preoperatively remain unclear. This study aimed to investigate the relationship between preoperative grip strength and postoperative upper extremity impairments in patients with breast cancer. Methods This retrospective cohort study included patients (age ≥ 18 years) with breast cancer who underwent mastectomy. Maximum grip strength was measured on the day before surgery. Upper extremity impairments were assessed 4–16 months after surgery using the Disabilities of the Arm, Shoulder and Hand (DASH) scale. Multiple linear regression analysis was used to evaluate the association between preoperative grip strength and postoperative upper extremity impairments. Results In total, 72 patients were included in the analysis. Multiple linear regression analysis showed that preoperative grip strength was significantly associated with the postoperative DASH score after adjusting for confounding factors (β = − 1.27, 95% confidence interval − 2.08 to − 0.48, p = 0.002). Conclusions This study showed that low preoperative grip strength is a risk factor for postoperative upper extremity impairments in patients with breast cancer. Providing prehabilitation to maintain and improve muscle strength immediately after diagnosis is important. Moreover, an individualized follow-up protocol according to preoperative screenings to prevent postoperative upper extremity impairments is necessary.

  Springer Science and Business Media LLC, Apr. 2025, Breast Cancer, 32(4) (4), 750 - 756, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Efficacy of Methionine Restriction and the PARP-inhibitor Olaparib and Their Combination onBRCA1Mutant and Wild-type Triple-negative Breast Cancer Cell Lines

  MAYUKO MIKI, SACHIKO INUBUSHI, QINGHONG HAN, SHOTARO INOUE, TOMONARI KUNIHISA, HIROKAZU TANINO, ROBERT M. HOFFMAN

  Lead, International Institute of Anticancer Research, Mar. 2025, Anticancer Research, 45(4) (4), 1367 - 1372, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Serum Exosomes Expressing CD9, CD63 and HER2 From Breast-Cancer Patients Decreased After Surgery of the Primary Tumor: A Potential Biomarker of Tumor Burden

  SACHIKO INUBUSHI, TOMONARI KUNIHISA, MARINA KUNIYASU, SHOTARO INOUE, MAYUKO YAMAMOTO, YUJI YAMASHITA, MAYUKO MIKI, SACHIKO MIZUMOTO, MOTOI BABA, ROBERT M. HOFFMAN, HIROKAZU TANINO

  International Institute of Anticancer Research, Oct. 2024, Cancer Genomics - Proteomics, 21(6) (6), 580 - 584, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Target-Oriented Classification of Triple-negative Breast Cancer

  SACHIKO MIZUMOTO, SACHIKO INUBUSHI, MAYUKO MIKI, HARUNA NAKAMURA, MOTOI BABA, YUJI YAMASHITA, MAYUKO YAMAMOTO, SHOTARO INOUE, HIROKAZU TANINO, TOMONARI KUNIHISA

  International Institute of Anticancer Research, Nov. 2023, Anticancer Research, 43(11) (11), 5067 - 5072, English

  [Refereed]

  Scientific journal


• Methionine Restriction Increases Exosome Production and Secretion in Breast Cancer Cells

  SACHIKO INUBUSHI, TOMONARI KUNIHISA, SACHIKO MIZUMOTO, SHOTARO INOUE, MAYUKO MIKI, ATSUSHI SUETSUGU, HIROKAZU TANINO, ROBERT M. HOFFMAN

  International Institute of Anticancer Research, Aug. 2023, Cancer Genomics - Proteomics, 20(5) (5), 412 - 416, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Rare Case of Bilateral Pectoralis Major Muscle Defect and Abnormal Muscle

  Mayuko Yamamoto, Tomonari Kunihisa, Motoi Baba, Sachiko Mizumoto, Yuji Yamashita, Mayuko Miki, Sachiko Inubushi, Aoi Okamoto, Ryuhei Fujimoto, Hirokazu Tanino

  A 56-year-old female patient with left breast cancer presented at our hospital. Preoperative CT scan showed an isolated bilateral pectoralis major muscle defect and abnormal muscle originating from the entire sternum and inserting in the lower ribs and rectus sheath. Total mastectomy and axillary lymph node dissection were performed. We believe that this case is unique and that others like it have never been reported. If there is a defect in the pectoralis major muscle, reconstructive surgery with a tissue expander is contraindicated. Therefore, preoperative evaluation of the chest wall musculature on imaging is recommended.

  S. Karger AG, Mar. 2022, Case Reports in Oncology, 15(1) (1), 351 - 355, English

  [Refereed]

  Scientific journal


• [A Case of Metastatic Recurrent Breast Cancer Successfully with Olaparib in Late Line Therapy].

  Misao Soyama, Tomonari Kunihisa, Natsumi Shimada, Haruna Suzuki, Yuna Ookawa, Aoi Okamoto, Mayuko Yamamoto, Mayuko Miki, Sachiko Mizumoto, Motoi Baba, Hirokazu Tanino

  A 44-years-old woman who underwent bilateral mastectomy was treated with chemotherapy after axillary lymph nodes and liver metastases recurrence. She was referred to our hospital for BRCA1/2 germline test and the test revealed BRCA2 pathogenic mutation. Before the administration of olaparib as the fourth-line therapy, liver dysfunction, caused by extensive liver metastasis, was observed. The liver damage improved, and tumor markers decreased immediately as shown in the blood test and CT examination results after 2 months; indicating marked reduction of liver metastasis. In the OlympiAD trial, the patients received olaparib as either the first-, second- or third-line treatment; however, few data on the efficacy of olaparib in the patients, as a late line treatment, were reported. In this article, we report a case of a woman in whom olaparib was used as the fourth-line treatment for metastatic recurrent breast cancer. A high therapeutic effect was obtained and the quality of life has been maintained in her for the past 1 year.

  Sep. 2021, Gan to kagaku ryoho. Cancer & chemotherapy, 48(9) (9), 1153 - 1155, Japanese, Domestic magazine

  Scientific journal


• Investigation of a Novel S-1 Administration Schedule for Treating Metastatic and Recurrent Breast Cancer (KBCOG13)

  MAYUKO MIKI, SHINTARO TAKAO, MUNEHARU KONISHI, YASUSHI SHIGEOKA, MASARU MIYASHITA, HIROFUMI SUWA, YASUO MIYOSHI, KOICHI HIROKAGA, TOSHITAKA OKUNO, KAZUHIKO YAMAGAMI, MICHIKO IMAMURA, KEIKO MURASE, AYAKO YANAI, HIROKAZU TANINO

  Lead, International Institute of Anticancer Research, Jun. 2021, Anticancer Research, 41(6) (6), 3121 - 3126, English

  [Refereed]

  Scientific journal


• Safety and Relative Dose Intensity of Dose-dense Doxorubicin and Cyclophosphamide Followed by Dose-dense Paclitaxel

  MEIKO NISHIMURA, TAKUMA ONOE, HIDEKI SAKAI, MINORI ARASE, SAYURI WATANABE, MISAO SOYAMA, KAZUKI HASHIMOTO, MAYUKO MIKI, KAORI TANE, KOICHI HIROKAGA, SHINTARO TAKAO, KOJI MATSUMOTO

  International Institute of Anticancer Research, Jul. 2019, Anticancer Research, 39(8) (8), 4379 - 4383, English

  [Refereed]

  Scientific journal


• Patient preference study comparing hypofractionated versus conventionally fractionated whole-breast irradiation after breast-conserving surgery

  Hiroki Kawaguchi, Kayoko Tsujino, Mayuko Miki, Yoko Matsumoto, Yosuke Ota, Koichi Hirokaga, Shintaro Takao, Toshinori Soejima, Ryohei Sasaki

  Oxford University Press (OUP), Mar. 2019, Japanese Journal of Clinical Oncology, 49(6) (6), 545 - 553, English

  [Refereed]

  Scientific journal


• [A case of locally advanced and metastatic breast cancer successfully treated with combination therapy of paclitaxel and bevacizumab].

  Mayuko Miki, Yoko Matsuo, Sachiko Yoshida, Kaori Tane, Koichi Hirokaga, Yoko Maekawa, Shintaro Takao

  The patient was a 53-year-old woman with a large left breast tumor. Triple-negative breast cancer with bilateral axillary lymph-node metastasis and bone metastasis was diagnosed. The primary treatment consisted of chemotherapy with a combination of paclitaxel(PTX 80mg/m 2)and bevacizumab(Bev 10 mg/kg). After chemotherapy, the tumor shrunk and showed an obvious decrease in size, eventually disappearing. After therapy, an ulcer was detected on the left breast, and mastectomy and axillary lymph node dissection were performed. The patient's postoperative course was uneventful and she has been receiving chemotherapy at our outpatient clinic. Thus, combination therapy with PTX and Bev may be useful for the local control of advanced breast cancer.

  Aug. 2014, Gan to kagaku ryoho. Cancer & chemotherapy, 41(8) (8), 1027 - 9, Japanese, Domestic magazine

  Scientific journal

• 乳がん患者を対象とした術前握力と術後上肢機能障害の関連に関する観察研究

  水田万裕, 奥村真帆, 井上順一朗, 上田雄也, 近藤心, 三木万由子, 國久智成, 小野玲, 小野玲, 酒井良忠, 秋末敏宏

  2024, 理学療法兵庫, (30) (30)