Research activity information

• Effects of Central Administration of Opioid Peptides, Vasotocin, Mesotocin, and Corticotrophin-Releasing Factor on Water Intake in Chicks

  Yuhui Zhang, Kaoruko Murata, Junya Takegaki, Takaoki Saneyasu, Kazuhisa Honda

  Mar. 2025, The Journal of Poultry Science, 62, jpsa.2025011, English

  [Refereed]


• Wet Feeding Promotes Growth without Affecting Hypothalamic Peptide Gene Expression in Growing Broiler Chicks

  Tomoya Matsunami, Yuhui Zhang, Yuji Taniguchi, Sei-ichi Hinomoto, Takaoki Saneyasu, Hiroshi Kamisoyama, Kazuhisa Honda

  Japan Poultry Science Association, Jan. 2025, The Journal of Poultry Science, 62, jpsa.2025008, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Recent Research on Mechanisms of Feeding Regulation in Chicks

  Takaoki Saneyasu

  Japan Poultry Science Association, Apr. 2024, The Journal of Poultry Science, 61, jpsa.2024012, English

  [Refereed][Invited]

  Scientific journal


• Effects of Amino Acid Supplementation to a Low-Protein Diet on the Growth Performance and Protein Metabolism-related Factors in Broiler Chicks

  Asmaa S. El-far, Maho Kamiya, Takaoki Saneyasu, Kazuhisa Honda

  Japan Poultry Science Association, Apr. 2024, The Journal of Poultry Science, 61, jpsa.2024014, English

  [Refereed]

  Scientific journal


• Periodic and Local LED Light Switching Induces the Locomotion of Broilers.

  Kusuda S, Ohsima C, Saneyasu T, Honda K

  Mar. 2024, The Journal of Poultry Science, 61, jpsa.2024009, English

  [Refereed]

  Scientific journal


• Effects of Dietary Brown Rice and Sake Lees on the Growth Performance and Color of Meat in Broiler Chicks

  Koki Nishikawa, Karin Miyazaki, Takehiro Hirai, Takaoki Saneyasu, Kazuhisa Honda

  Japan Poultry Science Association, Mar. 2024, The Journal of Poultry Science, 61, jpsa.2024007, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of Hypothalamic Transforming Growth Factor-β (TGF-β)/Smad Signaling in Feeding Regulation in Chickens

  Takaoki Saneyasu,Miku Ueda,Kanami Nagata,Jiawei Chai,Kazuhisa Honda,Hiroshi Kamisoyama

  Oct. 2022, The Journal of Poultry Science, 59(4) (4), 357 - 363, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Atrogin-1 knockdown inhibits the autophagy-lysosome system in mammalian and avian myotubes

  Takaoki Saneyasu, Kazuki Ogasawara, Yuki Fujiwara, Kazuhisa Honda, Hiroshi Kamisoyama

  Jun. 2022, Comparative Biochemistry and Physiology, Part A, 271, 111262, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• IGF-1 knockdown inhibits phosphorylation of Akt and ERK in chicken embryonic myotubes

  Takaoki Saneyasu, Tomonori Nakamura, Kazuhisa Honda, Hiroshi Kamisoyama

  Jun. 2022, Growth hormone & IGF research, 65, 101478, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparison of the effects of intracerebroventricular administration of glucagon-like peptides 1 and 2 on hypothalamic appetite regulating factors and sleep-like behavior in chicks

  Kewan A, Shimatani T, Saneyasu T, Kamisoyama H, Honda K

  Elsevier BV, Jan. 2022, Neuroscience Letters, 768, 136362 - 136362, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enzymatically-Synthesized Glycogen Induces Cecal Glucagon-Like Peptide-1 Production and Suppresses Food Intake in Mice

  Kazuhisa HONDA, Aki YASUHARA, Takaoki SANEYASU, Hiroshi KAMISOYAMA

  Center for Academic Publications Japan, Aug. 2021, Journal of Nutritional Science and Vitaminology, 67(4) (4), 217 - 224, English

  [Refereed]

  Scientific journal


• Effects of fasting and re-feeding on the expression of CCK, PYY, hypothalamic neuropeptides, and IGF-related genes in layer and broiler chicks

  Ahmed Kewan, Takaoki Saneyasu, Hiroshi Kamisoyama, Kazuhisa Honda

  Elsevier BV, Jul. 2021, Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 257, 110940 - 110940, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Central administration of insulin and refeeding lead to Akt and ERK phosphorylation in the chicken medulla

  Takaoki Saneyasu, Mizuki Ueno, Kanami Nagata, Ahmed Kewan, Kazuhisa Honda, Hiroshi Kamisoyama

  Elsevier BV, Jun. 2021, Neuroscience Letters, 136008 - 136008, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Central administration of insulin-like growth factor-2 suppresses food intake in chicks

  Kazuhisa Honda, Ahmed Kewan, Haruki Osada, Takaoki Saneyasu, Hiroshi Kamisoyama

  Elsevier BV, Apr. 2021, Neuroscience Letters, 751, 135797 - 135797, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of Corticosterone in Lipid Metabolism in Broiler Chick White Adipose Tissue

  Kazuhisa Honda, Kiyotaka Kurachi, Shoko Takagi, Takaoki Saneyasu, Hiroshi Kamisoyama

  Japan Poultry Science Association, 2021, The Journal of Poultry Science, 59(2) (2), 152 - 158, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of insulin-like growth factor-1 in the central regulation of feeding behavior in chicks

  Shoichi Fujita, Kazuhisa Honda, Mika Yamaguchi, Satoshi Fukuzo, Takaoki Saneyasu, Hiroshi Kamisoyama

  Japan Poultry Science Association, Nov. 2019, The Journal of Poultry Science, 56(4) (4), 270 - 276, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Myostatin Increases Smad2 Phosphorylation and Atrogin-1 Expression in Chick Embryonic Myotubes

  Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  Jul. 2019, The Journal of Poultry Science, 56(3) (3), 224 - 230, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Differential regulation of protein synthesis by skeletal muscle type in chickens

  Takaoki Saneyasu, Yuma Nakano, Nami Tsuchii, Tatsuya Tsuchihashi, Sayaka Kimura, Kazuhisa Honda, Hiroshi Kamisoyama

  2019, General and Comparative Endocrinology, 284, 113246, English

  [Refereed]

  Scientific journal


• Central administration of insulin and refeeding lead to the phosphorylation of AKT, but not FOXO1, in the hypothalamus of broiler chicks

  Takaoki Saneyasu, Satoshi Fukuzo, Ayana Kitashiro, Kanami Nagata, Kazuhisa Honda, Hiroshi Kamisoyama

  2019, Physiology & Behavior, 210, 112644, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of fasting and refeeding on the mRNA levels of insulin-like growth factor-binding proteins in chick liver and brain

  Shoichi Fujita, Mika Yamaguchi, Daichi Hiramoto, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  Japan Poultry Science Association, Nov. 2018, The Journal of Poultry Science, 55(4) (4), 269 - 273, English

  [Refereed]

  Scientific journal


• The extract of soybean protein increases slow-myosin heavy chain expression in C2C12 myotubes

  Takaoki Saneyasu, Haruka Shindo, Kazuhisa Honda, Hiroshi Kamisoyama

  Aug. 2018, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 64(4) (4), 296 - 300, English

  [Refereed]

  Scientific journal


• Hypothalamic Akt-mediated signaling regulates food intake in chicks

  Takaoki Saneyasu, Shoichi Fujita, Ayana Kitashiro, Satoshi Fukuzo, Kazuhisa Honda, Hiroshi Kamisoyama

  The central anorexigenic mechanism seems to be similar in mammals and chicks, because the appetite-suppressive action of a number of peptide hormones is similar in both species. Accumulating evidence in mammals has revealed that hypothalamic Akt-mediated signaling factors (for instance, mTOR and FOXO1) are significantly involved in the regulation of food intake. However, the role of hypothalamic Akt in feeding regulation is yet to be determined in chickens. In this study, we showed that pAkt (Thr308)/Akt, pFOXO1/FOXO1, and pS6 levels were significantly increased in the hypothalami of chicks refed 1 h after a 24 h-fast in correlation to increases in the plasma concentrations of insulin, one of the activators of the Akt-mediated signaling pathways. In addition, central administration of insulin increased the phosphorylation of Akt, FOXO1, and S6 in chicken hypothalami. Furthermore, intracerebroventricular injections of both phosphoinositide 3-kinase inhibitor LY294002 and mTOR inhibitor rapamyacin enhanced the food intake of chicks. These findings suggest that hypothalamic Akt-mediated signaling pathways contribute to the regulation of food intake in chicks.

  Elsevier Ireland Ltd, Mar. 2018, Neuroscience Letters, 670, 48 - 52, English

  [Refereed]

  Scientific journal


• 夜間における青色照明の点灯がブロイラーの行動，時計遺伝子発現，及び視床下部-下垂体-副腎皮質軸に及ぼす影響

  平本 大地, HONDA KAZUHISA, 近藤 真, 實安 隆興, 上曽山 博

  Nov. 2017, 日本家禽学会誌, 54(J2) (J2), J37 - J43, Japanese

  [Refereed]

  Scientific journal


• The IGF-1/Akt/S6 pathway and expressions of glycolytic myosin heavy chain isoforms are upregulated in chicken skeletal muscle during the first week after hatching

  Takaoki Saneyasu, Tatsuya Tsuchihashi, Ayana Kitashiro, Nami Tsuchii, Sayaka Kimura, Kazuhisa Honda, Hiroshi Kamisoyama

  Skeletal muscle mass is an important trait in the animal industry. We previously reported an age-dependent downregulation of the insulin-like growth factor 1 (IGF-1)/Akt/S6 pathway, major protein synthesis pathway, in chicken breast muscle after 1week of age, despite a continuous increase of breast muscle weight. Myosin heavy chain (HC), a major protein in muscle fiber, has several isoforms depending on chicken skeletal muscle types. HC I (fast-twitch glycolytic type) is known to be expressed in adult chicken breast muscle. However, little is known about the changes in the expression levels of protein synthesis-related factors and HC isoforms in perihatching chicken muscle. In the present study, protein synthesis-related factors, such as IGF-1 messenger RNA (mRNA) levels, phosphorylation of Akt, and phosphorylated S6 content, increased in an age-dependent manner after post-hatch day (D) 0. The mRNA levels of HC I, III and V (fast-twitch glycolytic type) dramatically increased after D0. The increase ratio of breast muscle weight was approximately 1100% from D0 to D7. To our knowledge, these findings provide the first evidence that upregulation of protein synthesis pathway and transcription of fast twitch glycolytic HC isoforms play critical roles in the increase of chicken breast muscle weight during the first week after hatching.

  WILEY, Nov. 2017, ANIMAL SCIENCE JOURNAL, 88(11) (11), 1779 - 1787, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of short-term fasting on the Akt-mediated pathway involved in protein metabolism in chicken skeletal muscle

  T. Saneyasu, N. Tsuchii, Y. Nakano, A. Kitashiro, T. Tsuchihashi, H. Shindo, K. Honda, H. Kamisoyama

  In the present study, we show that short-term (4 h) fasting significantly decreased the levels of protein synthesis-related factors such as the plasma insulin concentration, skeletal muscle pAkt, and pS6 levels in 2-wk-old chickens (P < 0.05). An intravenous injection of insulin significantly elevated the contents of pAkt and p-S6 in the skeletal muscle (P < 0.01). These findings suggest that decreasing the plasma insulin causes the down regulation of the Akt/S6 pathway in chicken skeletal muscle under short-term fasting conditions. However, protein synthesis was not significantly affected by short-term fasting. In addition, no significant change was observed in the levels of proteolysis-related factors such as plasma N-tau-methylhistidine, phosphorylated forkhead box class 0, and muscle ring finger-1 during, 4-h fasting, indicating that short-term fasting does not induce skeletal muscle proteolysis in chickens. Interestingly, atrogin-1 expression significantly increased after 2-h fasting (P < 0.05), and insulin injection significantly reversed the fasting-induced atrogin-1 expression in chicken skeletal muscle (P < 0.01). Collectively, these findings suggest that short-term fasting downregulates the insulin-stimulated Alct/S6 pathway but does not significantly affect protein synthesis and proteolysis in chicken skeletal muscle, and that atrogin-1 expression is upregulated in a FOX01-independent manners. (C) 2017 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Oct. 2017, DOMESTIC ANIMAL ENDOCRINOLOGY, 61(C) (C), 54 - 61, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Central and and peripheral administrations of insulin-like growth factor-1 suppress food intake in chicks

  Shoichi Fujita, Kazuhisa Honda, Daichi Hiramoto, Mei Gyu, Mika Okuda, Shungo Nakayama, Mika Yamaguchi, Takaoki Saneyasu, Hiroshi Kamisoyama

  A number of studies have been made on the physiological actions of insulin-like growth factor-1 (IGF-1) in mammals and birds. In mammals, the effects of central administration of IGF-1 on food intake have been examined. For example, intracerebroventricular administration of IGF-1 significantly decreased food intake in diabetic rats, but not in sheep and nondiabetic rats. The chicken is known to be a hyperglycemic animal. Like satiety hormones, plasma IGF-1 levels are elevated postprandially in chickens. In this study, we hypothesized that IGF-1 is involved in the regulation of food intake in chickens. Intracerebroventricular administration of IGF-1 significantly suppressed food intake in chicks in a dose dependent manner. Both the mRNAs of IGF-1 and its receptor were expressed throughout the brain. However, the mRNA levels of IGF-1 were not influenced by fasting and refeeding in all regions of the brain. On the other hand, 6 h of fasting significantly suppressed mRNA expression of hepatic IGF-1, and this effect was significantly reversed by 6 h of refeeding. Furthermore, in-vascular administration of IGF-1 significantly suppressed food intake in chicks. These findings suggest that IGF-1 may function as a satiety hormone in chickens.

  PERGAMON-ELSEVIER SCIENCE LTD, Oct. 2017, PHYSIOLOGY & BEHAVIOR, 179, 308 - 312, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of dietary heme iron and exercise training on abdominal fat accumulation and lipid metabolism in high-fat diet-fed mice

  Masanori Katsumura, Shoko Takagi, Hana Oya, Shohei Tamura, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  Animal by-products can be recycled and used as sources of essential nutrients. Water-soluble heme iron (WSHI), a functional food additive for supplementing iron, is produced by processing animal blood. In this study, we investigated the effects of dietary supplementation of 3% WSHI and exercise training for 4 weeks on the accumulation of abdominal fat and lipid metabolism in mice fed high-fat diet. Exercise-trained mice had significantly less perirenal adipose tissue, whereas WSHI-fed mice tended to have less epididymal adipose tissue. In addition, total weight of abdominal adipose tissues was significantly decreased in the Exercise + WSHI group. Dietary WSHI significantly increased the messenger RNA (mRNA) levels of lipoprotein lipase and hormone-sensitive lipase. WSHI-fed mice also tended to show increased mRNA levels of adipose triglyceride lipase in their epididymal adipose tissue. Dietary WSHI also significantly decreased the mRNA levels of fatty acid oxidation-related enzymes in the liver, but did not influence levels in the Gastrocnemius muscle. Exercise training did not influence the mRNA levels of lipid metabolism-related enzymes in the epididymal adipose tissue, liver or the Gastrocnemius muscle. These findings suggest that the accumulation of abdominal fat can be efficiently decreased by the combination of dietary WSHI and exercise training in mice fed high-fat diet.

  WILEY, Aug. 2017, ANIMAL SCIENCE JOURNAL, 88(8) (8), 1100 - 1106, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of continuous white light and 12 h white-12 h blue light-cycles on the expression of clock genes in diencephalon, liver, and skeletal muscle in chicks

  Kazuhisa Honda, Makoto Kondo, Daichi Hiramoto, Takaoki Saneyasu, Hiroshi Kamisoyama

  The core circadian clock mechanism relies on a feedback loop comprised of clock genes, such as the brain and muscle Arnt-like 1 (Bmal1), chriptochrome 1 (Cry1), and period 3 (Per3). Exposure to the light-dark cycle synchronizes the master circadian clock in the brain, and which then synchronizes circadian clocks in peripheral tissues. Birds have long been used as a model for the investigation of circadian rhythm in human neurobiology. In the present study, we examined the effects of continuous light and the combination of white and blue light on the expression of clock genes (Bmal1, Cry1, and Per3) in the central and peripheral tissues in chicks. Seventy two day-old male chicks were weighed, allocated to three groups and maintained under three light schedules: 12 h white light-12 h dark-cycles group (control); 24 h white light group (WW group); 12 h white light-12 h blue light-cycles group (WB group). The mRNA levels of clock genes in the diencephalon were significantly different between the control and WW groups. On the other hand, the alteration in the mRNA levels of clock genes was similar between the control and WB groups. Similar phenomena were observed in the liver and skeletal muscle (biceps femoris). These results suggest that 12 h white-12 h blue light-cycles did not disrupt the circadian rhythm of clock gene expression in chicks. (C) 2017 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, May 2017, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 207, 73 - 78, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gut Hormones and Regulation of Food Intake in Birds

  Kazuhisa Honda, Takaoki Saneyasu, Hiroshi Kamisoyama

  Gut hormones act as appetite regulatory hormones in mammals. For example, the hunger hormone ghrelin, which is released from the stomach before food intake, stimulates appetite. In contrast, satiety hormones such as cholecystokinin, glucagon-like peptide-1, and peptide YY, which are released from the intestines after food intake, suppress appetite. The effects of these peptides on food intake have been shown to be similar in both mammals and fishes. However, evidence suggests that the physiological roles of these gut hormones may be different between birds and other vertebrates. This review summarizes the current information on the roles of gut hormones in the regulation of food intake in birds, especially in chickens.

  JAPAN POULTRY SCIENCE ASSOC, Apr. 2017, JOURNAL OF POULTRY SCIENCE, 54(2) (2), 103 - 110, English

  [Refereed][Invited]

  Scientific journal


• Fasting and glucagon stimulate gene expression of pyruvate dehydrogenase kinase 4 in chickens

  Kazuhisa Honda, Shoko Takagi, Kiyotaka Kurachi, Haruka Sugimoto, Takaoki Saneyasu, Hiroshi Kamisoyama

  The excessive accumulation of body fat has become a serious problem in the broiler industry. However, the molecular mechanisms underlying the regulation of lipid metabolism-related genes in broiler chickens are not fully understood. In the present study, we investigated the role of glucagon on the expression of lipid metabolism-related genes in chicken white adipose tissue (WAT). Four hours of fasting significantly increased plasma levels of free fatty acid in broiler chickens. The mRNA levels of adipose triglyceride lipase (ATGL) and pyruvate dehydrogenase kinase 4 (PDK4) in abdominal WAT significantly increased by fasting, whereas the mRNA levels of diacylglycerol O-acyl-transferase homolog 2 (DGAT2) and peroxisome proliferator-activated receptor-γ (PPARγ) significantly decreased. The results suggest that fasting stimulates lipolysis and suppresses adipogenesis and re-esterification of TG in chicken WAT. Glucagon significantly increased the mRNA levels of PDK4 in chicken primary adipocytes, whereas there were no significant changes in the mRNA levels of ATGL, DGAT2, and PPARγ. Our findings suggest that glucagon upregulates PDK4 expression and may stimulate lipolysis without affecting the expression of ATGL in chicken WAT.

  Japan Poultry Science Association, 2017, Journal of Poultry Science, 54(4) (4), 292 - 295, English

  [Refereed]

  Scientific journal


• Identification, expression analysis, and functional characterization of peptide YY in chickens (Gallus gallus domesticus)

  Koji Aoki, Makoto Kondo, Mika Okuda, Takaoki Saneyasu, Honda Kazuhisa, Hiroshi Kamisoyama

  Elsevier, Jan. 2017, General and Comparative Endocrinology, 242, 11 - 17, English

  [Refereed]

  Scientific journal


• Effects of enzymatically synthesized glycogen and exercise on abdominal fat accumulation in high-fat diet-fed mice

  Shohei Tamura, Kazuhisa Honda, Ryoji Morinaga, Takaoki Saneyasu, Hiroshi Kamisoyama

  The combination of diet and exercise is the first choice for the treatment of obesity and metabolic syndrome. We previously reported that enzymatically synthesized glycogen (ESG) suppresses abdominal fat accumulation in obese rats. However, the effect of the combination of ESG and exercise on abdominal fat accumulation has not yet been investigated. Our goal in this study was therefore to evaluate the effects of dietary ESG and its combination with exercise on abdominal fat accumulation in high-fat diet (HFD)-fed mice. Male ICR mice were assigned to four groups: HFD, HFD containing 20% ESG, HFD with exercise, HFD containing 20% ESG with exercise. Treadmill exercise was performed for 3 wk (25 m/ min, 30 min/d, 3 d/wk) after 5-d adaption to running at that speed. Both ESG and exercise significantly reduced the weights of abdominal adipose tissues. In addition, the combination of ESG and exercise significantly suppressed abdominal fat accumulation, suggesting that ESG and exercise showed an additive effect. Exercise significantly increased the mRNA levels of lipid metabolism-related genes such as lipoprotein lipase, peroxisome proliferator-acti-vated receptor delta factor-delta (PPARδ), carnitin palmitoyltransferase b, adipose triglyceride lipase (ATGL), and uncoupling protein-3 in the gastrocnemius muscle. On the other hand, dietary ESG significantly decreased the mRNA levels of PPARd and ATGL in the gastrocnemius muscle. These results suggest that the combined treatment of ESG and exercise effectively suppresses abdominal fat accumulation in HFD-fed mice by different mechanisms.

  Center for Academic Publications Japan, 2017, Journal of Nutritional Science and Vitaminology, 63(6) (6), 405 - 411, English

  [Refereed]

  Scientific journal


• The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle

  Takaoki Saneyasu, Mariko Inui, Sayaka Kimura, Yu Yoshimoto, Nami Tsuchii, Haruka Shindo, Kazuhisa Honda, Hiroshi Kamisoyama

  The skeletal muscle mass is known to be controlled by the balance between protein synthesis and degradation. The fractional rate of protein synthesis has been reported to decrease age-dependently from 1 to 4 weeks of age in the chicken breast muscle (pectoralis major muscle). On the other hand, age-dependent change of the fractional protein degradation rate was reported to be less in the skeletal muscle of chickens. These findings suggest that protein synthesis is age-dependently downregulated in chicken muscle. We herein investigated the age-dependent changes in protein synthesis or proteolysis-related factors in the breast muscle of 7, 14, 28, and 49-day old broiler chickens. IGF-1 mRNA level, phosphorylation rate of Akt, and phospho-S6 content were coordinately decreased in an age dependent manner, suggesting that IGF-1-stimulated protein synthesis is downregulated with age in chicken breast muscle. In contrast, atrogin-1, one of the proteolysis-related factors, gradually increased with age at mRNA levels. However, plasma N-tau-methylhistidine concentration, an indicator of skeletal muscle proteolysis, did not coordinately change with atrogin-1 mRNA levels. Taken together, our results suggest that the IGF-1/Akt/S6 signaling pathway is age-dependently downregulated in the chicken breast muscle.

  JAPAN POULTRY SCIENCE ASSOC, Jul. 2016, JOURNAL OF POULTRY SCIENCE, 53(3) (3), 213 - 219, English

  [Refereed]

  Scientific journal


• Molecular Cues Guiding Matrix Stiffness in Liver Fibrosis

  Takaoki Saneyasu, Riaz Akhtar, Takao Sakai

  Tissue and matrix stiffness affect cell properties during morphogenesis, cell growth, differentiation, and migration and are altered in the tissue remodeling following injury and the pathological progression. However, detailed molecular mechanisms underlying alterations of stiffness in vivo are still poorly understood. Recent engineering technologies have developed powerful techniques to characterize the mechanical properties of cell and matrix at nanoscale levels. Extracellular matrix (ECM) influences mechanical tension and activation of pathogenic signaling during the development of chronic fibrotic diseases. In this short review, we will focus on the present knowledge of the mechanisms of how ECM stiffness is regulated during the development of liver fibrosis and the molecules involved in ECM stiffness as a potential therapeutic target for liver fibrosis.

  Lead, HINDAWI LTD, 2016, BIOMED RESEARCH INTERNATIONAL, 2016, 2646212, English

  [Refereed]

  Scientific journal


• Role of peroxisome proliferator-activated receptor alpha in the expression of hepatic fatty acid oxidation-related genes in chickens

  Kazuhisa Honda, Takaoki Saneyasu, Haruka Sugimoto, Kiyotaka Kurachi, Shoko Takagi, Hiroshi Kamisoyama

  Liver is the most important target organ for investigation of lipid metabolism in domestic fowls. However, little is known about the regulatory mechanism of fatty acid oxidation in chicken liver. In mammals, proliferator-activated receptor alpha (PPAR alpha), a transcription factor, plays an essential role in the regulation of hepatic fatty acid oxidation. The aim of the present study was to investigate the regulatory mechanisms of PPAR alpha-induced gene expression involved in hepatic fatty acid oxidation in chickens in vivo and in vitro. WY14643, a PPAR alpha agonist, significantly increased the messenger RNA (mRNA) levels of carnitine palmitoyltransferase 1a (CPT1a) and acyl-coenzyme A oxidase (ACO), but not long-, middle-and short-chain acyl-coenzyme A dehydrogenase (LCAD, MCAD and SCAD, respectively), hydroxyacyl-coenzyme A dehydrogenase (HAD), and PPARa itself in chicken hepatoma cells. In contrast, WY14643 significantly increased the mRNA levels of CPT1a, ACO, MCAD, SCAD, HAD and PPAR alpha in human hepatoma cells. The mRNA levels of CPT1a and ACO in the liver were significantly increased by 6 h of fasting in chickens, whereas the mRNA levels of LCAD, MCAD, SCAD and HAD were unchanged. These results suggest that, unlike in mammals, CPT1a and ACO might play an important role in PPAR alpha-induced fatty acid oxidation in the liver of chickens.

  WILEY-BLACKWELL, Jan. 2016, ANIMAL SCIENCE JOURNAL, 87(1) (1), 61 - 66, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Differential regulation of the expression of lipid metabolism-related genes with skeletal muscle type in growing chickens

  Takaoki Saneyasu, Sayaka Kimura, Ayana Kitashiro, Nami Tsuchii, Tatsuya Tsuchihashi, Mariko Inui, Kazuhisa Honda, Hiroshi Kamisoyama

  The regulatory mechanisms of carbohydrate and lipid metabolism are known to differ among skeletal muscle types in mammals. For example, glycolytic muscles prefer glucose as an energy source, whereas oxidative muscles prefer fatty adds (FA). We herein demonstrated differences in the expression of genes involved in carbohydrate and lipid metabolism in the pectoralis major (a glycolytic twitch muscle), adductor superficialis (an oxidative twitch muscle), and adductor profound (a tonic muscle) of 14-day-old chicks. Under ad libitum feeding conditions, the mRNA levels of muscle type phosphofructokinase-1 were markedly lower in the adductor superficialis muscle, suggesting that basal glycolytic activity is very low in this type of muscle. In contrast, high mRNA levels of lipoprotein lipase (LPL) and fatty acid translocase/cluster of differentiation 36 (FAT/CD36) in the adductor superficialis muscle suggest that FA uptake is high in this type of muscle. The mRNA levels of adipose triglyceride lipase (ATGL) and camitine palmitoyltransferase 1b (CPT1b) were significantly higher in the adductor profound muscle than in other muscles, suggesting that basal lipolytic activity is high in this type of muscle. Furthermore, the mRNA levels of peroxisome proliferator activated receptor 6 and CPT1b were significantly increased in the adductor superficialis muscle, but not in other muscles, after 24 h of fasting. Therefore, the availability of FA in the oxidative twitch muscles in growing chickens appears to be upregulated by fasting. Our results suggest that lipid metabolism-related genes are upregulated under both basal and fasting conditions in the adductor superficialis in growing chickens. (C) 2015 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Nov. 2015, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 189, 1 - 5, English

  [Refereed]

  Scientific journal


• Glucagon and Neuromedin U Suppress Food Intake in Broiler Chicks

  Kazuhisa Honda, Takaoki Saneyasu, Mika Okuda, Taku Uemura, Hiroshi Kamisoyama

  Several peptides show different effects on food intake between broiler and layer chicks. For example, the central administration of R-melanocyte-stimulating hormone decreased food intake in layer chicks but not in broiler chicks. The central administration of agouti-related protein increased food intake in layer chicks but not in broiler chicks. We previously reported that glucagon and neuromedin U (NMU) suppress food intake in layer chicks when administered centrally. However, the effects of these peptides on food intake in broiler chicks have not been examined. In the present study, we thus examined whether these peptides also show anorexigenic effects in broiler chicks. The intracerebroventricular administration of glucagon significantly suppressed food intake and increased plasma glucose concentration in broiler chicks. The hyperglycemic effect of glucagon had been shown in layer chicks in our previous studies. Intracerebroventricular administration of NMU significantly suppressed food intake and induced wing-flapping behavior in broiler chicks as well as in layer chicks. All our findings demonstrate that glucagon and NMU function as anorxigenic peptides in both broiler and layer chicks.

  JAPAN POULTRY SCIENCE ASSOC, Oct. 2015, JOURNAL OF POULTRY SCIENCE, 52(4) (4), 268 - 273, English

  [Refereed]

  Scientific journal


• Differences in the expression of genes involved in skeletal muscle proteolysis between broiler and layer chicks during food deprivation

  Takaoki Saneyasu, Sayaka Kimura, Mariko Inui, Yu Yoshimoto, Kazuhisa Honda, Hiroshi Kamisoyama

  Genetic selection results in a higher growth rate and meat yield in broiler chickens than in layer chickens. We herein demonstrated differences in the effects of 24 h of fasting on the expression of genes involved in skeletal muscle proteolysis between broiler and layer chicks. The mRNA levels of proteolysis-related genes were analyzed in the pectoralis major muscle of 14-day-old chicks after 0 or 24 h of fasting. The mRNA levels of ubiquitin ligases such as atrogin-1 and muscle RING finger-1 (MuRF-1) as well as transcription factor forkhead box class O (FOXO) 1 were significantly increased by fasting in broiler and layer chicks, suggesting that the FOXO1-induced ubiquitin-proteasome system, a major proteolytic system in skeletal muscles, was activated by fasting in both chicks. The mRNA levels of atrogin-1 were significantly lower in broiler chicks than in layer chicks after fasting. Furthermore, the mRNA levels of insulin-like growth factor-1 were significantly decreased by fasting in layer chicks, but not in broiler chicks. The mRNA levels of FOXO3 were significantly increased by fasting in layer chicks, but not in broiler chicks. Therefore, the ubiquitin-proteasome system did not appear to have been fully upregulated in broiler chicks. These results suggest that differences in the expression of genes related to the ubiquitin-proteasome system in skeletal muscle proteolysis between broiler and layer chicks during food deprivation are one of the causes of the high growth rate in broiler chickens. (C) 2015 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Aug. 2015, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 186, 36 - 42, English

  [Refereed]

  Scientific journal


• Glucagon-like Peptide-2 Functions as an Anorexigenic Peptide not only in the Central Nervous System but also in the Peripheral Circulation in Broiler Chicks

  Kazuhisa Honda, Tomohiko Shimatani, Koji Aoki, Takuya Yamaguchi, Makoto Kondo, Takaoki Saneyasu, Hiroshi Kamisoyama

  Brain-gut peptides play important roles in the appetite regulatory system in mammals. Glucagon-like peptide (GLP)-1, GLP-2, and oxyntomodulin (OXM) are processed from the same precursor, proglucagon, in both brain and intestines in mammals and birds. We previously showed that intracerebroventricular administration of these three peptides significantly suppressed food intake in chicks. However, peripheral roles of chicken GLP-2 have not yet been investigated, although GLP-2 plays important roles both in central and peripheral regulation of food intake in mammals. The aim of this study is to investigate whether GLP-2 functions as an anorexigenic peptide in both brain and peripheral circulation in chicks. Intracerebroventricular administration of GLP-2 significantly suppressed food intake in chicks. Twenty-four hours of fasting significantly decreased the mRNA level of proglucagon in the medulla oblongata of chicks. These results suggest that GLP-2 functions as anorexigenic peptides in the central nervous system in chicks. In addition, intravenous administration of GLP-2 significantly suppressed food intake in chicks. Lines of evidence suggest that dietary nutrients stimulate the secretion of GLP-2 from L cells in the small intestine in chickens. These findings suggest that GLP-2 functions as both central and peripheral anorexigenic signals in chicks.

  JAPAN POULTRY SCIENCE ASSOC, Jul. 2015, JOURNAL OF POULTRY SCIENCE, 52(3) (3), 183 - 187, English

  [Refereed]

  Scientific journal


• Correlation analysis of hypothalamic mRNA levels of appetite regulatory neuropeptides and several metabolic parameters in 28-day-old layer chickens

  Kazuhisa Honda, Takaoki Saneyasu, Koji Aoki, Tomohiko Shimatani, Takuya Yamaguchi, Hiroshi Kamisoyama

  Various lines of evidence suggest that appetite-related neuropeptides in the hypothalamus are regulated by adiposity signals such as leptin and insulin in mammals. In the present study, we examined age-dependent changes in the weight of abdominal fat and hypothalamic mRNA levels of neuropeptide Y (NPY, an orexigenic neuropeptide) and proopiomelanocortin (POMC, a precursor of anorexigenic neuropeptides) in growing chickens at 7, 14, 21 and 28 days of age. Hypothalamic NPY mRNA levels were significantly (P<0.05) decreased after 14 days of age, whereas hypothalamic POMC mRNA levels were significantly (P<0.05) increased at 28 days of age. The percentage of abdominal fat was significantly increased after 14 days of age in chickens. We next examined the correlation of hypothalamic NPY and POMC mRNA levels and several parameters at 28 days of age. There were no significant correlations between hypothalamic mRNA levels of NPY or POMC and the percentage of abdominal fat. These findings suggest that the gene expressions of NPY and POMC do not depend on adiposity in chickens, at least in 28-day-old layer chickens.

  WILEY, May 2015, ANIMAL SCIENCE JOURNAL, 86(5) (5), 517 - 522, English

  [Refereed]

  Scientific journal


• Intracerebroventricular administration of chicken glucagon-like peptide-2 potently suppresses food intake in chicks

  Kazuhisa Honda, Takaoki Saneyasu, Tomohiko Shimatani, Koji Aoki, Takuya Yamaguchi, Kiwako Nakanishi, Hiroshi Kamisoyama

  Glucagon-related peptides, such as glucagon-like peptide (GLP)-1, GLP-2 and oxyntomodulin (OXM), are processed from an identical precursor proglucagon. In mammals, all of these peptides are suggested to be involved in the central regulation of food intake. We previously showed that intracerebroventricular administration of chicken OXM and GLP-1 significantly suppressed food intake in chicks. Here, we show that central administration of chicken GLP-2 potently suppresses food intake in chicks. Male 8-day-old chicks (Gallus gallus domesticus) were used in all experiments. Intracerebroventricular administration of chicken GLP-2 significantly suppressed food intake in chicks. Plasma glucose concentration was significantly decreased by chicken GLP-2, whereas plasma nonesterified fatty acid concentration was significantly increased. Intracerebroventricular administration of chicken GLP-2 did not affect plasma corticosterone concentration. In addition, the anorexigenic effect of GLP-2 was not reversed by the corticotropin-releasing factor (CRF) receptor antagonist -helical CRF, suggesting that CRF is not a downstream mediator of the anorexigenic pathway of GLP-2 in chicks. Intracerebroventricular administration of an equimolar amount of GLP-1 and GLP-2, but not OXM, significantly suppressed food intake in both broiler and layer chicks. All our findings suggest that GLP-2 functions as a potent anorexigenic peptide in the brain, as well as GLP-1, in chicks.

  WILEY, Mar. 2015, ANIMAL SCIENCE JOURNAL, 86(3) (3), 312 - 318, English

  [Refereed]

  Scientific journal


• Dietary mannanase-hydrolyzed copra meal improves growth and increases muscle weights in growing broiler chickens

  Masahisa Ibuki, Yu Yoshimoto, Mariko Inui, Kensuke Fukui, Hiroko Yonemoto, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  The utilization of copra meal as a feed ingredient is limited because it contains a high level of mannan. However, recent findings indicate that the effect of copra meal on growth performance in broiler chickens can be improved by the supplementation of mannanase in the diet. In the present study, we examined the effect of mannanase-hydrolyzed copra meal (MCM) on growth performance and muscle protein metabolism in growing broiler chickens (Gallus gallus domesticus). Forty 8-day-old male broiler chicks were assigned to two groups (four birds in each pen, five replicates) and fed either a commercial diet (as a control diet) or a diet containing MCM at 0.2% until 22 days of age. Dietary MCM significantly increased the weights of body, breast muscle, and thighs in chickens, whereas the weights of abdominal adipose tissue and liver were not affected. Cumulative feed intake was significantly increased by MCM. Dietary MCM significantly decreased plasma 3-methylhistidine level. The messenger RNA and protein levels of muscle protein metabolism-related factors were not altered by MCM. These findings suggest that the growth-promoting effect of MCM is related to the suppression of muscle proteolysis in growing broiler chickens.

  WILEY, May 2014, ANIMAL SCIENCE JOURNAL, 85(5) (5), 562 - 568, English

  [Refereed]

  Scientific journal


• Intracerebroventricular administration of novel glucagon-like peptide suppresses food intake in chicks

  Kazuhisa Honda, Takaoki Saneyasu, Takuya Yamaguchi, Tomohiko Shimatani, Koji Aoki, Kiwako Nakanishi, Hiroshi Kamisoyama

  Glucagon-related peptides such as glucagon, glucagon-like peptide-1, and oxyntomodulin suppress food intake in mammals and birds. Recently, novel glucagon-like peptide (GCGL) was identified from chicken brain, and a comparatively high mRNA expression level of GCGL was detected in the hypothalamus. A number of studies suggest that the hypothalamus plays a critical role in the regulation of food intake in mammals and birds. In the present study, we investigated whether GCGL is involved in the central regulation of food intake in chicks. Male 8-day-old chicks (Gallus gallus) were used in all experiments. Intracerebroventricular administration of GCGL in chicks significantly suppressed food intake. Plasma glucose level was significantly decreased by GCGL, whereas plasma corticosterone level was not affected. Central administration of a corticotrophin-releasing factor (CRF) receptor antagonist, alpha-helical CRF, attenuated GCGL-suppressed food intake. It seems likely that CRF receptor is involved in the GCGL-induced anorexigenic pathway. All our findings suggest that GCGL functions as an anorexigenic peptide in the central nervous system of chicks. (C) 2013 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Feb. 2014, PEPTIDES, 52, 98 - 103, English

  [Refereed]

  Scientific journal


• Intracerebroventricular administration of chicken oxyntomodulin suppresses food intake and increases plasma glucose and corticosterone concentrations in chicks

  Kazuhisa Honda, Takaoki Saneyasu, Takuya Yamaguchi, Tomohiko Shimatani, Koji Aoki, Kiwako Nakanishi, Hiroshi Kamisoyama

  Elsevier Inc., Feb. 2014, Neuroscience Letters, 564(3) (3), 57 - 61, English

  [Refereed]

  Scientific journal


• Age-dependent changes in the mRNA levels of neuropeptide Y, proopiomelanocortin, and corticotropin-releasing factor in the hypothalamus in growing broiler chicks

  Takaoki Saneyasu, Kiwako Nakanishi, Hiroyuki Atsuta, Atsushi Ikura, Shin Hasegawa, Hiroshi Kamisoyama, Kazuhisa Honda

  Lead, Japan Poultry Science Association, Oct. 2013, Journal of Poultry Science, 50(4) (4), 364 - 369, English

  [Refereed]

  Scientific journal


• Effects of short-term refeeding on the expression of genes involved in lipid metabolism in chicks (Gallus gallus)

  Takaoki Saneyasu, Miho Shiragaki, Kiyotaka Kurachi, Hiroshi Kamisoyama, Kazuhisa Honda

  The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to feeding in chicks. A total of 18 thirteen day-old male chicks were fasted for 12 h. The mRNA levels of the genes in the liver and white adipose tissue were analyzed after 0, 2, and 4 h of refeeding. The mRNA levels of sterol regulatory element-binding protein (SREBP) 1, liver X receptor alpha, peroxisome proliferator-activated receptor (PPAR) gamma, acetyl-CoA carboxylase alpha and fatty acid synthase were significantly increased after 2 h of refeeding. In contrast, the mRNA levels of PPAR alpha and carnitine palmitoyltransferase 1a were significantly decreased after 2 h of refeeding. The mRNA level of acyl-CoA oxidase was significantly decreased after 4 h of refeeding. The mRNA levels of cholesterol metabolism-related genes such as SREBP2 and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased after 2 h of refeeding. In the white adipose tissue, the mRNA level of PPAR gamma was significantly increased after 2 h of refeeding, whereas the mRNA level of adipose triglyceride lipase was significantly decreased after 4 h of refeeding. These results demonstrated that expression of lipid metabolism-related genes is regulated by short-term refeeding in chicks. (C) 2013 Elsevier Inc. All rights reserved.

  Lead, ELSEVIER SCIENCE INC, Sep. 2013, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 166(1) (1), 1 - 6, English

  [Refereed]

  Scientific journal


• Effect of Licorice Flavonoid Oil on Cholesterol Metabolism in High Fat Diet Rats

  Kazuhisa HONDA, Takaoki SANEYASU, Shin HASEGAWA, Yuji TOMINAGA, Shinichi YOKOTA, Hiroshi KAMISOYAMA

  Oxford University Press (OUP), Jun. 2013, Bioscience, Biotechnology, and Biochemistry, 77(6) (6), 1326 - 1328, English

  [Refereed]

  Scientific journal


• Effects of short term fasting on the expression of genes involved in lipid metabolism in chicks

  Takaoki Saneyasu, Miho Shiragaki, Kohei Nakanishi, Hiroshi Kamisoyama, Kazuhisa Honda

  The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to short term fasting in chicks (Gallus gallus). The mRNA level of the genes was analyzed after 0, 2, and 4 h of fasting in the liver and white adipose tissue. In the liver, the mRNA level of peroxisome proliferator-activated receptor a was significantly increased after 2 h of fasting. The mRNA levels of carnitine palmitoyltransferase la and acyl-CoA oxidase were significantly increased after 4 h of fasting. In contrast, the mRNA levels of sterol regulatory element-binding protein 1, acetyl-CoA carboxylase alpha, and fatty acid synthase were significantly decreased after 4 h of fasting. The mRNA levels of cholesterol metabolism-related genes such as 3-hydroxy-3-methylglutaryl-CoA reductase and cholesterol 7 alpha-hydroxylase were significantly decreased after 4 h of fasting. In the white adipose tissue, the mRNA level of adipose triglyceride lipase was significantly increased after 4 h of fasting. In contrast, the mRNA levels of peroxisome proliferator-activated receptor gamma and lipoprotein lipase were significantly decreased after 4 h of fasting. These results demonstrated that the gene expression of lipid metabolism-related genes is regulated by short term fasting in both the liver and WAT in chicks. (C) 2013 Elsevier Inc. All rights reserved.

  Lead, ELSEVIER SCIENCE INC, Jun. 2013, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 165(2) (2), 114 - 118, English

  [Refereed]

  Scientific journal


• Dietary coenzyme Q10 suppressed hepatic hydroxymethylglutaryl-CoA reductase activity in laying hens

  Kazuhisa Honda, Takaoki Saneyasu, Tohru Motoki, Yuriko Park, Hiroshi Kamisoyama

  The effects of dietary coenzyme Q10 (CoQ10) on cholesterol metabolism in laying hens were investigated. Dietary CoQ10 significantly reduced egg yolk cholesterol content and suppressed hepatic hydroxymethylglutaryl-CoA reductase (HMGR) activity. It is therefore likely that CoQ10 acts as an HMGR inhibitor in the livers of laying hens, which in turn results in a reduction in egg-yolk cholesterol.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2013, Bioscience, Biotechnology and Biochemistry, 77(7) (7), 1572 - 1574, English

  [Refereed]

  Scientific journal


• A comparative study of the central effects of melanocortin peptides on food intake in broiler and layer chicks

  Kazuhisa Honda, Takaoki Saneyasu, Shin Hasegawa, Hiroshi Kamisoyama

  Broiler chicks eat more food than layer chicks. However, the causes of the difference in food intake in the neonatal period between these strains are not clear. In this study, we examined the involvement of proopiomelanocortin (POMC)-derived melanocortin peptides alpha-, beta- and gamma-melanocyte-stimulating hormones (MSHs) in the difference in food intake between broiler and layer chicks. First, we compared the hypothalamic mRNA levels of POMC between these strains and found that there was no significant difference in these levels between broiler and layer chicks. Next, we examined the effects of central administration of MSHs on food intake in these strains. Central administration of alpha-MSH significantly suppressed food intake in both strains. Central administration of beta-MSH significantly suppressed food intake in layer chicks, but not in broiler chicks, while central administration of gamma-MSH did not influence food intake in either strain. It is therefore likely that the absence of the anorexigenic effect of beta-MSH might be related to the increased food intake in broiler chicks. (c) 2012 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Sep. 2012, PEPTIDES, 37(1) (1), 13 - 17, English

  [Refereed]

  Scientific journal


• Neuropeptide Y effect on food intake in broiler and layer chicks

  Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama, Atsushi Ikura, Yoko Nakayama, Shin Hasegawa

  Broiler chicks eat more food than layer chicks. In this study, we examined the involvement of orexigenic peptide neuropeptide Y (NPY) in the difference in food intake between broiler and layer chicks (Gallus gallus). First, we compared the hypothalamic mRNA levels of NPY and its receptors (Y1 and Y5 receptors) between these strains at 1, 2, 4, and 8 days of age. Daily food intake was significantly higher in broiler chicks than layer chicks after 2 days of age. However, the hypothalamic NPY mRNA level was significantly lower in broiler chicks than layer chicks except at 8 days of age. In addition, the mRNA levels of NPY receptors were also significantly lower in broiler chicks than layer chicks at 2 and 4 days of age (Y1 receptor) or 2 days of age (Y5 receptor). These results suggest that the differences in the expressions of hypothalamic NPY and its receptors do not cause the increase in food intake in broiler chicks. To compare the orexigenic effect of NPY between broiler and layer chicks, we next examined the effects of central administration of NPY on food intake in these strains. In both strains, central administration of NPY significantly increased food intake at 2,4 and 8 days of age. All our findings demonstrated that the increase in food intake in broiler chicks is not accompanied with the over-expression of NPY or its receptor. (C) 2011 Elsevier Inc. All rights reserved.

  Lead, ELSEVIER SCIENCE INC, Aug. 2011, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 159(4) (4), 422 - 426, English

  [Refereed]

  Scientific journal


• Alpha-melanocyte stimulating hormone plays an important role in the regulation of food intake by the central melanocortin system in chicks

  Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama, Yoko Nakayama, Kengo Ikegami, Shin Hasegawa

  Proopiomelanocortin (POMC, a precursor of melanocortin peptides) neurons in the hypothalamus play an important role in the central regulation of food intake in mammals. There is evidence that human melanocortin peptides alpha-, beta- and gamma2-melanocyte-stimulating hormone (alpha-, beta- and gamma 2-MSH) significantly decreased food intake in chickens. However, the amino acid sequences of beta- and gamma 2-MSH of chickens are different from those of humans whereas the amino acid sequence of alpha-MSH is conserved between these species. In the present study, we examined the effects of the central administration of alpha-, chicken beta-, and chicken gamma 2-MSH on food intake in chicks. Central administration of alpha-MSH significantly suppressed food intake in chicks. In contrast, beta- and gamma 2-MSH did not influence food intake in chicks. Central administration of HS014, a melanocortin 4 receptor antagonist, significantly reversed the anorexigenic action of alpha-MSH, suggesting that this action is mediated by the melanocortin 4 receptor in chicks as well as in mammals. These results suggest that alpha-MSH may play an important role in the regulation of food intake by the central melanocortin system in chicks. (C) 2011 Elsevier Inc. All rights reserved.

  Lead, ELSEVIER SCIENCE INC, May 2011, PEPTIDES, 32(5) (5), 996 - 1000, English

  [Refereed]

  Scientific journal


• Effect of Dietary Coenzyme Q10 on Cholesterol Metabolism in Growing Chickens

  Kazuhisa Honda, Hiroshi Kamisoyama, Tomoki Motoori, Takaoki Saneyasu, Shin Hasegawa

  Coenzyme Q10 (CoQ10), a potent lipophilic antioxidant, is a naturally occurring compound with a ubiquitous distribution in nature and is used as a dietary supplement to combat aging. In rats, there is evidence that coenzyme Q9, major coenzyme Q homologue in rodents, suppresses hepatic cholestcrogenesis and decreases plasma total cholesterol concentration. In this study, we investigated the effect of dietary CoQ10 on cholesterol metabolism in growing chickens. The supplementation of CoQ10 in a diet significantly decreased the total cholesterol levels in the liver and plasma. Plasma very low density lipoprotein (VLDL) cholesterol concentration was significantly decreased by dietary CoQ10. The enzymatic activity of hepatic hydroxymethylglutaryl-coenzyme A reductase (HMGR), the rate-limiting enzyme of the cholesterol synthetic pathway, was significantly decreased by dietary CoQ10, whereas the mRNA level of HMGR was not affected. These findings suggest that dietary CoQ10 suppresses hepatic cholesterogenesis by the inhibition of HMGR activity at the posttranscriptional level in chickens, which in turn decreases plasma VLDL cholesterol concentration.

  JAPAN POULTRY SCIENCE ASSOC, Jan. 2010, JOURNAL OF POULTRY SCIENCE, 47(1) (1), 41 - 47, English

  [Refereed]

  Scientific journal


• Corticotropin-releasing factor is a downstream mediator of the beta-melanocyte-stimulating hormone-induced anorexigenic pathway in chicks

  Hiroshi Kamisoyama, Kazuhisa Honda, Takaoki Saneyasu, Kunio Sugahara, Shin Hasegawa

  Proopiomelanocortin (POMC, a precursor of anorexigenic neuropeptides) neurons in hypothalamus suppresses food intake in both mammals and chickens. In mammals, several lines of evidence suggest that POMC-derived anorexigenic peptides upregulate mRNA levels of anorexigenic peptides such as corticotropin-releasing factor (CRF) and thyrotropin-releasing factor and downregulate mRNA levels of orexigenic peptides such as orexin and melanin-concentrating hormone. However, the POMC-induced anorexigenic pathway in chickens has not been well characterized. In the present study, we investigated how POMC neurons regulate mechanisms of food intake using an anorexigenic peptide, beta-melanocyte-stimulating hormone (beta-MSH), derived from the post-transcriptional cleavage of POMC. Central administration of beta-MSH in chicks significantly suppressed food intake, and importantly, this suppression was accompanied by a significant upregulation of CRF mRNA levels. Furthermore, the CRF type 2 receptor antagonist alpha-helical CRF significantly reversed the anorexigenic action of beta-MSH. These findings indicate that CRF and its receptor, CRF type 2 receptor, act as the major mediators in beta-MSH-induced anorexigenic action in chicks. beta-MSH significantly increased orexin mRNA levels and did not alter mRNA levels of thyrotropin-releasing factor and melanin-concentrating hormone in chicks, suggesting that the beta-MSH-induced anorexigenic pathway in chicks is different from that in mammals. Increases in orexin mRNA levels were accompanied by significant decreases in plasma glucose concentration, suggesting that orexin mRNA might be stimulated by beta-MSH-induced hypoglycemia. Thus, this study demonstrates the direct evidence that CRF is a critical downstream target in the beta-MSH-induced anorexigenic pathway in chicks. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER IRELAND LTD, Jul. 2009, NEUROSCIENCE LETTERS, 458(3) (3), 102 - 105, English

  [Refereed]

  Scientific journal


• Central administration of insulin suppresses food intake in chicks

  Kazuhisa Honda, Hiroshi Karnisoyama, Takaoki Saneyasu, Kunio Sugahara, Shin Hasegawa

  Although the orexigenic action of peptide hormones such as ghrelin and growth hormone releasing peptide is different between chickens and mammals, the anorexigenic action of peptide hormones is similar in both species. For example, central administration of peptide hormones such as leptin, cholecystokinin or glucagon has been shown to suppress food intake behavior in chickens and mammals. Central administration of insulin Suppresses food intake in mammals. However, the anorexigenic action of insulin in chickens has not yet been identified. In the present study, we investigated the effects of central administration of insulin on food intake in chicks. Intracerebroventricular administration of insulin in chicks significantly suppressed food intake. Central administration of insulin significantly upregulated mRNA levels of proopiomelanocortin (POMC), cocaine- and amphetamine-regulated transcript (CART) and corticotropin-releasing factor (CRF), but did not influence mRNA levels of neuropeptide Y and agouti-related protein in the hypothalamus. These results suggest that alpha-melanocyte stimulating hormone (alpha-MSH, an anorexigenic peptide from the post-translational cleavage of POMC), CART and CRF are involved in the anorexigenic action of insulin in chicks. Furthermore, central administration of alpha-MSH or CART significantly suppressed food intake. In addition, alpha-MSH significantly upregulated CRF rnRNA expression, suggesting that the anorexigenic action of alpha-MSH is mediated by CRF. Our findings demonstrate that insulin functions in chicks as an appetite-suppressive peptide in the central nervous system and suggest that this anorexigenic action is mediated by CART, alpha-MSH and CRF. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER IRELAND LTD, Aug. 2007, NEUROSCIENCE LETTERS, 423(2) (2), 153 - 157, English

  [Refereed]

  Scientific journal


• Central administration of neuromedin U suppresses food intake in chicks

  Hiroshi Kamisoyama, Kazuhisa Honda, Takaoki Saneyasu, Kunio Sugahara, Shin Hasegawa

  The appetite-suppressive action of brain-gut peptides is similar in both chickens and mammals. In mammals, the brain-gut peptide neuromedin U (NMU) suppresses food intake via hypothalamic neuropeptides, corticotropin-releasing factor (CRF), oxytocin, and arginine-vasopressin. In chickens, central administration of CRF, oxytocin, or arginine-vasotocin (AVT, a nonmammalian equivalent of arginine-vasopressin) suppresses food intake. However. the anorexigenic action of NMU in chickens has not yet been identified. In the present study, we analyzed the effects of the central administration of NMU on food intake and hypothalamic mRNA levels of CRF, AVT and mesotocin (a nonmammalian equivalent of oxytocin) in chicks. Intracerebroventricular administration of NMU in chicks significantly suppressed food intake and induced wing-flapping behavior. NMU also significantly upregulated mRNA expression of CRF and AVT, but did not influence mRNA expression of mesotocin in the hypothalamus. These results suggest that NMU functions as an appetite-suppressive peptide via CRF and AVT in the central nervous system in chicks. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER IRELAND LTD, Jun. 2007, NEUROSCIENCE LETTERS, 420(1) (1), 1 - 5, English

  [Refereed]

  Scientific journal

• Search and Evaluation of the Slow-type Muscle-increasing Factors Derived from Soybean

  實安 隆興, 進藤 悠, 本田 和久, 上曽山 博

  不二たん白質研究振興財団, Jun. 2017, 大豆たん白質研究, 19(37) (37), 147 - 152, Japanese


• 豚由来機能性食品素材に関する研究の最新動向

  HONDA KAZUHISA, SANEYASU TAKAOKI, KAMISOYAMA HIROSHI

  日本畜産振興会, Jun. 2014, 養豚の友, 6(543) (543), 43 - 45, Japanese

  [Invited]

  Introduction commerce magazine

• Mechanisms of Collagen Network Organization in Response to Tissue/Organ Damage. In: Travascio (ed), Composition and Function of the Extracellular Matrix in the Human Body

  Takaoki Saneyasu, Saeko Yoshioka, Takao Sakai

  Others, InTech, Jun. 2016, English

  Scholarly book

• Effects of amino acid supplementation to a rice-based low-protein diet on the growth performance in broiler chicks

  Risa Migita, Junya Takegaki, Takaoki Saneyasu, Kazuhisa Honda

  The 2025 Spring Meeting of Japan Poultry Science Association, Mar. 2025, Japanese

  Oral presentation


• Effects of hypertonic saline administration on protein and lipid metabolism-related gene expression in chicks

  Kaoruko Murata, Yuhui Zhang, Junya Takegaki, Takaoki Saneyasu, Kazuhisa Honda

  The 2025 Spring Meeting of Japan Poultry Science Association, Mar. 2025, Japanese

  Oral presentation


• Is phosphorylation of medullary p38 MAPK involved in induction of postprandial satiety in chicks?

  Miyu Kaihatsu, Junya Takegaki, Kazuhisa Honda, Takaoki Saneyasu

  The 2025 Spring Meeting of Japan Poultry Science Association, Mar. 2025, Japanese

  Oral presentation


• 食欲抑制ホルモンの中枢投与や絶食・再給餌がニワトリ中枢の炎症性サイトカインの遺伝子発現に及ぼす影響

  北山藍香, 本田和久, 實安隆興

  令和5年度第73回関西畜産学会大会, Nov. 2023

  Oral presentation


• L-137の給与がブロイラーのLPS誘導性の反応に及ぼす影響

  坊ケ内恵麻, 實安隆興, 本田和久

  令和5年度第73回関西畜産学会大会, Nov. 2023

  Oral presentation


• 照明色を用いたブロイラーの移動行動の誘導が産肉量、肉質、及び脚の発達に及ぼす影響

  楠田慎吾, 大島用三, 實安隆興, 本田和久

  令和5年度第73回関西畜産学会大会, Nov. 2023

  Oral presentation


• 玄米酒粕飼料の給与期間の違いがブロイラーの成長成績と肉質に及ぼす影響

  西川幸樹, 清水綾香, 平井猛博, 實安隆興, 本田和久

  令和5年度第73回関西畜産学会大会, Nov. 2023

  Oral presentation


• 玄米主体飼料における酒粕の配合割合の違いがブロイラーの成長成績と肉色に及ぼす影響

  本田和久, 西川幸樹, 清水綾香, 平井猛博, 實安隆興

  日本家禽学会2023年度秋季大会, Sep. 2023

  Oral presentation


• 玄米酒粕主体飼料の産卵鶏への給与が鶏卵の卵質に及ぼす影響

  本田和久, 清水綾香, 西川幸樹, 平井猛博, 實安隆興

  日本畜産学会第131回大会, Sep. 2023

  Oral presentation


• 種々の神経ペプチドの中枢投与がニワトリヒナの飲水に及ぼす影響

  本田和久, Yuhui Zhang, 松波知弥, 谷口悠二, 樋本清一, 黒木悟, 實安隆興

  日本家禽学会2023年度春季大会, Mar. 2023

  Oral presentation


• 玄米と酒粕の給与がブロイラーの肉質に及ぼす影響

  本田和久, 西川幸樹, 宮﨑香綸, 平井猛博, 實安隆興

  第64回日本食肉科学会大会, Mar. 2023

  Oral presentation


• インスリン様成長因子（IGF）-1 がニワトリ筋管細胞のIGF 関連遺伝子の発現に及ぼす影響

  本田和久, 長田晴季, 實安隆興

  日本家禽学会2022年度秋季大会, Sep. 2022

  Oral presentation


• 照明を用いたブロイラーの移動行動の誘導

  楠田慎吾, 大島用三, 實安隆興, 本田和久

  日本家禽学会2022年度秋季大会, Sep. 2022

  Oral presentation


• L-137 の給与がブロイラーの腸管免疫関連遺伝子の発現に及ぼす影響

  坊ケ内恵麻, 三山景子, 實安隆興, 本田和久

  日本家禽学会2022年度秋季大会, Sep. 2022

  Oral presentation


• Effects of brown rice and sake lees on growth performance and meat quality in broiler chickens

  西川幸樹, 宮﨑香綸, 本田和久, 平井猛博, 広畑修二, 實安隆興, 上曽山博

  The 2022 Spring Meeting of Japan Poultry Science Association, Mar. 2022

  Oral presentation


• Effects of periodic and regional changing of lighting color on the behavior of broilers

  楠田慎吾, 中村恒志, 本田和久, 大島用三, 實安隆興, 上曽山博

  The 2022 Spring Meeting of Japan Poultry Science Association, Mar. 2022

  Oral presentation


• Studies on the role of hypothalamic TGF-β/Smad signaling pathway in regulation of food intake in chickens

  上田実来, 永田加菜美, 本田和久, 上曽山博, 實安隆興

  The 2022 Spring Meeting of Japan Poultry Science Association, Mar. 2022

  Oral presentation


• Interaction of glucagon-related peptides or melanocortins in the regulation of food intake in chicks

  Ahmed Kewan, Kazuhisa Honda, Takaoki Saneyasu, Hiroshi Kamisoyama

  The 2022 Spring Meeting of Japan Poultry Science Association, Mar. 2022

  Oral presentation


• 玄米の給与期間の違いがブロイラー及び地鶏の肉質に及ぼす影響

  宮崎香綸, アルナ, 實安隆興, 本田和久, 上曽山博

  令和３年度 第７１回関西畜産学会神戸大会, Oct. 2021


• ブロイラーの延髄ではp38MAPKが摂食抑制に関与している可能性がある

  永田加菜実, 本田和久, 上曽山博, 實安隆興

  令和３年度 第７１回関西畜産学会神戸大会, Oct. 2021


• 飼料と水の混合給餌がブロイラーの産肉量と肉質に及ぼす影響

  松波知弥, 本田和久, 谷口悠二, 樋本清一, 黒木悟, 實安隆興, 上曽山博

  日本家禽学会2021年度春季大会, Mar. 2021


• 絶食がニワトリの肝臓及び骨格筋のインスリン様成長因子結合タンパク質の発現に及ぼす影響

  長田晴季, 本田和久, Ahmed Kewan, 實安隆興, 上曽山博

  日本家禽学会2021年度春季大会, Mar. 2021


• IGF-1のノックダウンがニワトリ筋管細胞のAkt及びErkのリン酸化に及ぼす影響

  中村友紀, 本田和久, 上曽山博, 實安隆興

  日本家禽学会2021年度春季大会, Mar. 2021


• 加熱殺菌された乳酸菌L-137の給与がブロイラーの成長成績に及ぼす影響

  本田和久, 三山景子, 實安隆興, 上曽山博

  日本家禽学会2020年度春季大会, Mar. 2020


• 青色照明が地鶏の時計遺伝子Bmal 1の発現に及ぼす影響

  中村恒志, 實安隆興, 本田和久, 上曽山博

  日本家禽学会2020年度春季大会, Mar. 2020


• 玄米の給与が種々の肉用鶏の肉質に及ぼす影響

  宮下基, 田中真由子, 井通貫太, 實安隆興, 本田和久, 上曽山博

  日本家禽学会2020年度春季大会, Mar. 2020


• 小豆タンパク質の給与が高脂肪食給与マウスの糖・脂質代謝に及ぼす影響

  坂本奈津子, 森永涼司, 實安隆興, 本田和久, 上曽山博

  第58回日本栄養・食糧学会近畿支部大会, Nov. 2019


• 青色照明の半日点灯がブロイラーの摂食量及び間脳・肝臓・骨格筋における種々の遺伝子発現に及ぼす影響

  本田和久, 平本大地, 近藤真, 實安隆興, 上曽山博

  日本畜産学会第126回大会, Sep. 2019


• 日齢及び性別の違いが地鶏の肉質に及ぼす影響

  HONDA KAZUHISA, 宮下 基, 田中 真由子, 實安 隆興, 上曽山 博

  日本畜産学会第125回大会, Mar. 2019, Japanese, 麻布大学, Domestic conference

  Oral presentation


• 玄米主体飼料の給与がブロイラー及び地鶏の産肉量と肉質に及ぼす影響

  HONDA KAZUHISA, 宮下 基, 田中 真由子, 實安 隆興, 上曽山 博

  日本家禽学会2019年度春季大会, Mar. 2019, Japanese, 麻布大学, Domestic conference

  Oral presentation


• Effect of myostatin on atrogin-1 expression in chicken myotubes

  SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2018年度秋季大会, Sep. 2018, Japanese, 東北大学, Domestic conference

  Oral presentation


• ニワトリにおいて、延髄Erkシグナリングは摂食抑制に関与している

  上野 瑞城, 福造 哲, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成30年度第68回関西畜産学会大会, Sep. 2018, Japanese, 徳島大学, Domestic conference

  Oral presentation


• インスリンの中枢投与がブロイラーの視床下部Akt/FOXO及びPOMCの遺伝子発現に及ぼす影響

  福造 哲, 上野 瑞城, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成30年度第68回関西畜産学会大会, Sep. 2018, Japanese, 徳島大学, Domestic conference

  Oral presentation


• 飼料用米の給与がブロイラーの産肉量及び肉質に及ぼす影響

  HONDA KAZUHISA, 平本 大地, 宮下 基, 實安 隆興, 上曽山 博

  日本畜産学会第124回大会, Mar. 2018, Japanese, 東京大学, Domestic conference

  Oral presentation


• 飼料用米の給与がひょうご味どりの産肉量及び肉質に及ぼす影響

  HONDA KAZUHISA, 平本 大地, 宮下 基, 實安 隆興, 上曽山 博

  日本家禽学会2018年度春季大会, Mar. 2018, Japanese, 東京大学, Domestic conference

  Oral presentation


• 小豆給与と運動の負荷が高脂肪食給与マウスの腹部脂肪蓄積に及ぼす影響

  森永 涼司, 田村 翔平, 實安 隆興, 本田 和久, 上曽 山博

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンの給与はマウスの盲腸におけるグルカゴン様ペプチ

  田村 翔平, 森永 涼司, 安原 亜希, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, 龍谷大学, Domestic conference

  Oral presentation


• 絶食がニワトリの種々の骨格筋におけるタンパク質合成に及ぼす影響の比較

  YUMA NAKANO, NAMI TSUCHII, SATOSHI FUKUZO, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成29年度 第67回関西畜産学会大阪大会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• 飼料摂取がブロイラーとレイヤーにおける消化管ホルモンの遺伝子発現に及ぼす影響

  HONDA KAZUHISA, 中山 駿吾, 奥田 実加, 實安 隆興, 上曽山 博

  第123回日本畜産学会大会, Sep. 2017, Japanese, 信州大学, Domestic conference

  Oral presentation


• ニワトリの摂食調節機構におけるアグーチシグナリングタンパクの役割

  HONDA KAZUHISA, 牛 萌, 青木 昂史, 實安 隆興, 上曽山 博

  日本家禽学会2017年度秋季大会, Sep. 2017, Japanese, 信州大学, Domestic conference

  Oral presentation


• ニワトリにおいて、視床下部インスリンシグナル伝達経路は摂食抑制に関与している

  SATOSHI FUKUZO, SHOICHI FUJITA, AYANA KITASHIRO, MIZUKI UENO, YUMA NAKANO, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成29年度 第67回関西畜産学会大阪大会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• インスリンの中枢投与や再給餌がブロイラーの視床下部インスリンシグナル伝達経路に及ぼす影響

  SATOSHI FUKUZO, MIZUKI UENO, YUMA NAKANO, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  日本家禽学会2017年度秋季大会, Sep. 2017, Japanese, 日本家禽学会, 信州大学, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンが脂肪負荷マウスの血中トリグリセリド濃度及び高脂肪食給与マウスの腸内細菌叢に及ぼす影響

  田村 翔平, 森永 涼司, 實安 隆興, 本田 和久, 上曽山 博

  第71回日本栄養・食料学会大会, May 2017, Japanese, 沖縄コンベンションセンター, Domestic conference

  Poster presentation


• 種々のホルモンがニワトリの肝臓及び脂肪組織における脂質代謝関連遺伝子の発現に及ぼす影響

  髙木 聖子, 杉本 はるか, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第40回鳥類内分泌研究会大会, Nov. 2016, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山（愛媛県松山市）, Domestic conference

  Oral presentation


• インスリン様成長因子及びアイリシンの中枢投与がニワトリの摂食に及ぼす影響

  HONDA KAZUHISA, 藤田 昇一, 奥田 実加, 實安 隆興, 上曽山 博

  第40回鳥類内分泌研究会大会, Nov. 2016, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山（愛媛県松山市）, Domestic conference

  Oral presentation


• 短時間の絶食がブロイラーの脂肪組織トリグリセリド代謝関連遺伝子の発現に及ぼす影響

  髙木 聖子, 杉本 はるか, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第66回関西畜産学会大会, Oct. 2016, Japanese, 関西畜産学会, 香川大学農学部（香川県高松市）, Domestic conference

  Oral presentation


• 青色照明がブロイラーの肝臓及び骨格筋における時計遺伝子の発現に及ぼす影響

  平本 大地, 近藤 真, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第66回関西畜産学会大会, Oct. 2016, Japanese, 関西畜産学会, 香川大学農学部（香川県高松市）, Domestic conference

  Oral presentation


• 青色照明がニワトリの時計遺伝子の発現に及ぼす影響

  近藤真, 平本大地, 實安隆興, HONDA KAZUHISA, 上曽山博

  日本家禽学会2016年度秋季大会, Sep. 2016, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館（静岡県静岡市）, Domestic conference

  Oral presentation


• インスリン様成長因子-2の中枢投与はニワトリヒナの摂食を抑制する

  HONDA KAZUHISA, 牛 萌, 平本 大地, 藤田 昇一, 奥田 実加, 實安 隆興, 上曽山 博

  日本家禽学会2016年度秋季大会, Sep. 2016, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館（静岡県静岡市）, Domestic conference

  Oral presentation


• インスリン様成長因子-1の中枢投与はニワトリヒナの摂食を抑制する

  平本大地, 牛萌, 藤田昇一, 奥田実加, 實安隆興, HONDA KAZUHISA, 上曽山博

  日本家禽学会2016年度秋季大会, Sep. 2016, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館（静岡県静岡市）, Domestic conference

  Oral presentation


• 牛肉エキスエタノール抽出画分の給与がラットの腹部脂肪蓄積に及ぼす影響

  KAZUHISA HONDA, TAKAOKI SANEYASU, HIROSHI KAMISOYAMA

  第70回日本栄養・食糧学会大会, May 2016, Japanese, 公益社団法人日本栄養・食糧学会, 武庫川女子大学, Domestic conference

  Oral presentation


• 短時間の絶食がブロイラーの骨格筋におけるタンパク質代謝関連因子の発現量に及ぼす影響

  TSUCHII NAMI, KITASHIRO AYANA, TSUCHIHASHI TATSUYA, SHIDO HARUKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Oral presentation


• 新生ヒナの骨格筋におけるタンパク質合成関連因子の発現変動

  TSUCHIHASHI TATSUYA, KIMURA SAYAKA, KITASHIRO AYANA, TSUCHII NAMI, SHINDO HARUKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Oral presentation


• ニワトリにおけるインスリンによる摂食抑制に視床下部mTORは関与していない

  KITASHIRO AYANA, TSYCHII NAMI, TSUCHIHASHI TATSUYA, SHIDO HARUKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Oral presentation


• Peptide YY functions as an anorexgenic peptide in the peripheral circulation in chicks

  Koji Aoki, Tomohiko Shimatani, Meng Niu, Makoto Kondo, Mika Okuda, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  第40回日本比較内分泌学会大会, Dec. 2015, Japanese, 日本比較内分泌学会, JMSアステールプラザ（広島県広島市）, Domestic conference

  Poster presentation


• 水溶性ヘム鉄の給与と運動の負荷が高脂肪食給与マウスの脂肪組織におけるトリグリセリド代謝及び炎症関連遺伝子の発現に及ぼす影響

  勝村仁智, 實安隆興, 本田 和久, 上曽山博

  第54回日本栄養・食糧学会近畿支部大会, Oct. 2015, Japanese, 日本栄養・食糧学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンの給与と運動の負荷が高脂肪食給与マウスに及ぼす影響

  田村翔平, 勝村仁智, 實安隆興, 本田 和久, 上曽山博

  第54回日本栄養・食糧学会近畿支部大会, Oct. 2015, Japanese, 日本栄養・食糧学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• 発光ダイオード照明の点灯時間の違いがブロイラーの成長に及ぼす影響

  近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 日本家禽学会, 酪農学園大学, Domestic conference

  Oral presentation


• 短期間のコルチコステロン給与が新生ヒナに及ぼす影響

  大屋華, 近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 日本家禽学会, 酪農学園大学, Domestic conference

  Oral presentation


• 青色発光ダイオード照明がニワトリヒナの骨格筋形成或いはタンパク質代謝関連遺伝子の発現に及ぼす影響

  近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本畜産学会第120回大会, Sep. 2015, Japanese, 日本畜産学会, 酪農学園大学, Domestic conference

  Oral presentation


• 水溶性ヘム鉄の給与と運動の負荷が高脂肪食給与マウスに及ぼす影響

  勝村仁智, 實安隆興, 本田和久, 上曽山博

  平成27年度（第65回）関西畜産学会大会, Sep. 2015, Japanese, 関西畜産学会, 愛媛大学, Domestic conference

  Oral presentation


• 飼料へのコルチコステロンの添加がニワトリヒナの骨格筋の形成或いはタンパク質代謝関連遺伝子の発現に及ぼす影響

  大屋華, 近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本畜産学会第120回大会, Sep. 2015, Japanese, 日本畜産学会, 酪農学園大学, Domestic conference

  Oral presentation


• コルチコステロンはニワトリの脂肪組織トリグリセリドリパーゼ遺伝子の発現を促進する

  髙木聖子, 杉本はるか, 倉地清隆, 青木達哉, 實安隆興, 本田和久, 上曽山博

  平成27年度（第65回）関西畜産学会大会, Sep. 2015, Japanese, 関西畜産学会, 愛媛大学, Domestic conference

  Oral presentation


• 青色発光ダイオード照明がニワトリヒナの血中コルチコステロン濃度に及ぼす影響

  HONDA KAZUHISA, MAKOTO KONDO, MIKA OKUDA, SANEYASU TAKAOKI, KAMISOYAMA HIROSHI

  日本家禽学会2015年度春季大会, Mar. 2015, Japanese, 日本家禽学会, 宇都宮大学, Domestic conference

  Oral presentation


• 種々のニワトリの骨格筋における脂質代謝特性の比較

  SAYAKA KIMURA, AYANA KITASHIRO, TATSUYA TSUCHIHASHI, NAMI TSUCHII, MARIKO INUI, SANEYASU TAKAOKI, KAZUHISA HONDA, HIROSHI KAMISOYAMA

  日本畜産学会第119回大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮大学, Domestic conference

  Oral presentation


• ブロイラーにおける骨格筋タンパク質合成関連因子の遺伝子発現量の成長に伴う変化

  SAYAKA KIMURA, MARIKO INUI, TATSUYA TSUCHIHASHI, AYANA KITASHIRO, NAMI TSUCHII, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2015年度春季大会, Mar. 2015, Japanese, 日本家禽学会, 宇都宮大学, Domestic conference

  Oral presentation


• ニワトリにおけるグルカゴン様ペプチド-1及び2の摂食抑制機構へのAMPKの関与

  TOMOHIKO SHIMATANI, KOJI AOKI, 牛 萌, TAKUYA YAMGUCHI, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2015年度春季大会, Mar. 2015, Japanese, 日本家禽学会, 宇都宮大学, Domestic conference

  Oral presentation


• グルカゴン様ペプチド-2 の摂食抑制効果はエキセンジン（5-39）によって緩和される

  TOMOHIKO SHIMATANI, KOJI AOKI, 牛 萌, TAKUYA YAMGUCHI, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本畜産学会第119回大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮大学, Domestic conference

  Oral presentation


• 絶食がブロイラーの浅胸筋及び長内転筋におけるタンパク質代謝関連遺伝子の発現に及ぼす影響

  KIMURA SAYAKA, INUI MARIKO, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• 水溶性ヘム鉄の給与が高脂肪食給与マウスの脂質代謝に及ぼす影響

  KATSUMURA MASANORI, SUGIMOTO HARUKA, KURACHI KIYOTAKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  平成26年度（第64回）関西畜産学会大会, Sep. 2014, Japanese, 広島大学, Domestic conference

  Oral presentation


• ニワトリ肝臓脂肪酸酸化関連遺伝子の発現調節におけるペルオキシソーム増殖剤活性化受容体αの役割

  SUGIMOTO HARUKA, KURACHI KIYOTAKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• ニワトリにおける視床下部摂食調節ペプチドの種々のパラメーターとの関係

  AOKI KOJI, SHIMATANI TOMOHIKO, YAMAGUCHI TAKUYA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• グルカゴン様ペプチド-1及び-2の静脈内投与がニワトリヒナの摂食に及ぼす影響

  SHIMATANI TOMOHIKO, AOKI KOJI, YAMAGUCHI TAKUYA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• 絶食がレイヤー及びブロイラーの骨格筋タンパク質代謝関連遺伝子の発現に及ぼす影響

  SANEYASU TAKAOKI, INUI MARIKO, KIMURA SAYAKA, YOSHIMOTO YU, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度春季大会, Mar. 2014, Japanese, 日本家禽学会, 茨城県つくば市, Domestic conference

  Oral presentation


• ブロイラーの浅胸筋及び長内転筋における骨格筋タンパク質代謝関連因子の遺伝子発現の比較

  SANEYASU TAKAOKI, HONDA KAZUHISA, INUI MARIKO, YOSHIMOTO YU, KIMURA SAYAKA, KAMISOYAMA HIROSHI

  日本畜産学会第118回大会, Mar. 2014, Japanese, 日本畜産学会, 茨城県つくば市, Domestic conference

  Poster presentation


• グルカゴン様ペプチド-2の中枢投与はブロイラーヒナの摂食を抑制する

  HONDA KAZUHISA, SANEYASU TAKAOKI, YAMAGUCHI TAKUYA, AOKI KOJI, SHIMATANI TOMOHIKO, NAKANISHI KIWAKO, KAMISOYAMA HIROSHI

  日本家禽学会2014年度春季大会, Mar. 2014, Japanese, 日本家禽学会, 茨城県つくば市, Domestic conference

  Oral presentation


• オキシントモジュリンの中枢投与はブロイラーヒナの摂食を抑制する

  HONDA KAZUHISA, SANEYASU TAKAOKI, YAMAGUCHI TAKUYA, AOKI KOJI, SHIMATANI TOMOHIKO, NAKANISHI KIWAKO, KAMISOYAMA HIROSHI

  日本畜産学会第118回大会, Mar. 2014, Japanese, 日本畜産学会, 茨城県つくば市, Domestic conference

  Oral presentation


• 二品種のニワトリの成長に伴う視床下部食欲調節関連神経ペプチドの遺伝子発現の比較

  中西 貴和子, 實安 隆興, 以倉 淳史, HONDA KAZUHISA, 長谷川 信, 上曽山 博

  日本家禽学会2012年度秋季大会, Sep. 2012, Japanese, 日本家禽学会, 香川県高松市, Domestic conference

  Oral presentation


• 種々のホルモンがニワトリの肝臓及び脂肪組織における脂質代謝関連遺伝子の発現に及ぼす影響

  髙木聖子, 杉本はるか, SANEYASU Takaoki, HONDA Kazuhisa, 上曽山博, KAMISOYAMA Hiroshi

  第40回鳥類内分泌研究会大会, Nov. 2001, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山, Domestic conference

  Oral presentation


• インスリン様成長因子及びアイリシンの中枢投与がニワトリの摂食に及ぼす影響

  HONDA Kazuhisa, 藤田昇一, 奥田実加, SANEYASU Takaoki, 上曽山博, KAMISOYAMA Hiroshi

  第40回鳥類内分泌研究会大会, Nov. 2001, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山, Domestic conference

  Oral presentation


• 短時間の絶食がブロイラーの脂肪組織トリグリセリド代謝関連遺伝子の発現に及ぼす影響

  髙木聖子, 杉本はるか, SANEYASU Takaoki, HONDA Kazuhisa, 上曽山博, KAMISOYAMA Hiroshi

  第66回関西畜産学会大会, Oct. 2001, Japanese, 関西畜産学会, 香川大学農学部, Domestic conference

  Oral presentation


• 青色照明がブロイラーの肝臓及び骨格筋における時計遺伝子の発現に及ぼす影響

  平本大地, 近藤真, SANEYASU Takaoki, HONDA Kazuhisa, 上曽山博, KAMISOYAMA Hiroshi

  第66回関西畜産学会大会, Oct. 2001, Japanese, 関西畜産学会, 香川大学農学部, Domestic conference

  Oral presentation


• インスリン様成長因子-2の中枢投与はニワトリヒナの摂食を抑制する

  HONDA Kazuhisa, 牛萌, 平本大地, 藤田昇一, 奥田実加, SANEYASU Takaoki, 上曽山博, KAMISOYAMA Hiroshi

  日本家禽学会2016年度秋季大会, Sep. 2001, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館, Domestic conference

  Oral presentation

• 関西畜産学会

  - Present


• 日本家禽学会

  - Present


• 日本畜産学会

  - Present

• 中枢のストレス応答MAPキナーゼがニワトリの食欲調節に及ぼす影響

  實安 隆興

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2023 - 31 Mar. 2026


• MRTFが筋管細胞のインスリンシグナル、及び糖・脂質代謝調節に及ぼす影響

  公益財団法人木下記念事業団, 木下基礎科学研究基金助成事業, May 2023 - Jul. 2024, Principal investigator


• 骨格筋の成長と炎症予防（抑制）を目的としたPGC-1αアイソフォームの発現制御に関する研究（II）

  公益財団法人伊藤記念財団, 令和5年度公益財団法人伊藤記念財団助成, Apr. 2023 - Mar. 2024, Principal investigator


• 骨格筋の成長と炎症予防（抑制）を目的としたPGC-1αアイソフォームの発現制御に関する研究

  公益財団法人伊藤記念財団, 令和4年度公益財団法人伊藤記念財団助成, Apr. 2022 - Mar. 2023, Principal investigator


• Roles of central cellular signaling factors in feeding regulation of chickens

  Kamisoyama Hiroshi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2019 - Mar. 2022, Coinvestigator

  The present study suggests the following points; An activation of central Smad signaling suppresses food intake in chickens, but it might not occur under physiological conditions. In layer (egg type) chickens, upregulation of Akt and ERK signaling in the medulla oblongata suppresses food intake, and insulin is involved in the upregulation. On other hand, Akt and p38MAPK, but not ERK, in the medulla oblongata are involved in feeding regulation in broiler (meat type) chickens. Akt and ERK in the medulla oblongata are not involved in suppression of food intake induced by cholecystokinin, glucagon-like peptide (GLP)-1, and GLP-2.


• 骨格筋のタンパク質代謝におけるatrogin-1の役割

  一般財団法人旗影会, 一般助成, Apr. 2020 - Mar. 2021, Principal investigator

  Competitive research funding


• Differential physiological roles between insulin and IGF-1

  Saneyasu Takaoki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Kobe University, Apr. 2018 - Mar. 2020, Principal investigator

  To clarify effects of skeletal muscle-derived IGF-1 on muscle differentiation and regulation of protein metabolism, I examined the expression of muscle differentiation-related genes and phosphorylation rates of Akt and Erk in chicken embryonic myoblasts and myotubes treated wiht IGF-1 siRNA. The treatment of IGF-1 siRNA significantly decreased the mRNA levels in the myoblasts and the phosphorylation rates in the myotubes. These results suggest that skeletal muscle-derived IGF-1 plays an important role in regulating the expression of muslce differentiation-related genes and protein metabolism in the skeletal muscle.

  Competitive research funding


• ニワトリ骨格筋におけるミオスタチン／Smadシグナリングの解明

  實安 隆興

  一般財団法人旗影会, Apr. 2017 - Mar. 2018, Principal investigator

  Competitive research funding


• 筋管細胞におけるMRTFの役割に関する研究

  實安 隆興

  公益財団法人ひょうご科学技術協会, 学術研究助成, Apr. 2016 - Mar. 2017, Principal investigator

  Competitive research funding


• 豚肝臓由来ペプチドが肝線維化に及ぼす影響

  實安 隆興

  公益財団法人伊藤記念財団, 研究助成, 2016 - Mar. 2017, Principal investigator

  Competitive research funding


• 肉用鶏と卵用鶏におけるインスリンシグナル伝達機構の比較

  實安 隆興

  学術研究助成基金助成金／若手研究(B), Apr. 2015 - Mar. 2017, Principal investigator

  Competitive research funding


• 實安 隆興

  公益社団法人不二たん白研究振興財団, 研究助成（若手研究者枠）, Apr. 2015 - Mar. 2016, Principal investigator

  Competitive research funding


• 畜産物由来ペプチドの肝障害改善の効果について

  實安 隆興

  公益財団法人伊藤記念財団, 研究助成, 2015 - Mar. 2016, Principal investigator

  Competitive research funding