Research activity information

• Construction of a versatile in vitro cultivation screening platform using human oral microbiota.

  Kengo Sasaki, Yasunobu Takeshima, Ayami Fujino, Junya Yamashita, Akira Kimoto, Daisuke Sasaki, Akihiko Kondo, Masaya Akashi, Ryo Okumura

  We developed a simulation model of human oral microbiota using Bio Palette oral medium (BPOM) containing 0.02% glucose and lower bacterial nitrogen sources, derived from saliva and dental plaque. By decreasing the concentration of Gifu anaerobic medium (GAM) from 30 to 10 g L-1 , we observed increased ratios of target pathogenic genera, Porphyromonas and Fusobacterium from 0.5% and 1.7% to 1.2% and 3.5%, respectively, in the biofilm on hydroxyapatite (HA) discs. BPOM exhibited the higher ratios of Porphyromonas and Fusobacterium, and amplicon sequence variant number on HA, compared with GAM, modified GAM and basal medium mucin. Mixing glycerol stocks of BPOM culture solutions from four human subjects resulted in comparable ratios of these bacteria to the original saliva. In this simulation model, sitafloxacin showed higher inhibitory effects on P. gingivalis than minocycline hydrochloride at a low dosage of 0.1 μg mL-1 . Probiotics such as Streptococcus salivarius and Limosilactobacillus fermentum also showed significant decreases in Porphyromonas and Fusobacterium ratios on HA, respectively. Overall, the study suggests that BPOM with low carbon and nutrients could be a versatile platform for assessing the efficacy of antibiotics and live biotherapeutics in treating oral diseases caused by Porphyromonas and Fusobacterium.

  Apr. 2024, Environmental microbiology reports, 16(2) (2), e13243, English, International magazine

  Scientific journal


• Sulfated and non-sulfated chondroitin affect the composition and metabolism of human colonic microbiota simulated in an in vitro fermentation system

  Kentaro Inokuma, Daisuke Sasaki, Kaoru Kurata, Megumi Ichikawa, Yuya Otsuka, Akihiko Kondo

  Abstract Chondroitin sulfate (CS) is a family of glycosaminoglycans and have a wide range of applications in dietary supplements and pharmaceutical drugs. In this study, we evaluated the effects of several types of CS, differing in their sulfated positions, on the human colonic microbiota and their metabolites. CS (CSA, CSC, and CSE) and non-sulfated chondroitin (CH) were added into an in vitro human colonic microbiota model with fecal samples from 10 healthy individuals. CS addition showed a tendency to increase the relative abundance of Bacteroides, Eubacterium, and Faecalibacterium, and CSC and CSE addition significantly increased the total number of eubacteria in the culture of the Kobe University Human Intestinal Microbiota Model. CSE addition also resulted in a significant increase in short-chain fatty acid (SCFA) levels. Furthermore, addition with CSC and CSE increased the levels of a wide range of metabolites including lysine, ornithine, and Ile-Pro-Pro, which could have beneficial effects on the host. However, significant increases in the total number of eubacteria, relative abundance of Bacteroides, and SCFA levels were also observed after addition with CH, and the trends in the effects of CH addition on metabolite concentrations were identical to those of CSC and CSE addition. These results provide novel insight into the contribution of the colonic microbiota to the beneficial effects of dietary CS.

  Springer Science and Business Media LLC, Jul. 2023, Scientific Reports, 13(1) (1)

  Scientific journal


• Effects of partially hydrolyzed guar gums of different molecular weights on a human intestinal in vitro fermentation model.

  Daisuke Sasaki, Kengo Sasaki, Aya Abe, Makoto Ozeki, Akihiko Kondo

  Partially hydrolyzed guar gums (PHGGs) are prebiotic soluble dietary fibers. High molecular-weight PHGGs have rapid fermentation and high short-chain fatty acid (SCFA) productivity rates, compared to low molecular-weight PHGGs. Therefore, low molecular-weight PHGGs may have less pronounced prebiotic effects than high molecular-weight PHGGs. However, the effects of PHGGs of different molecular weights on the human intestinal microbiota, as well as their fermentation ability and prebiotic effects, have not been investigated. The aim of this study was to evaluate the effects of two PHGGs of different molecular weights, Sunfiber-R (SF-R; 20 kDa) and Sunfiber-V (SF-V; 5 kDa), on human colonic microbiota and SCFA production. A human intestinal in vitro fermentation model was operated by fecal samples with and without the PHGGs. The addition of 0.2% SF-R or SF-V increased the relative abundance of Bacteroides spp., especially that of Bacteroides uniformis. This increase corresponded to a significant (p = 0.030) and non-significant (p = 0.073) increase in propionate production in response to SF-R and SF-V addition, respectively. Both fibers increased the relative abundance of Faecalibacterium and stimulated an increase in the abundance of unclassified Lachnospiraceae and Bifidobacterium. In conclusion, the low molecular-weight PHGG exerted prebiotic effects on human colonic microbiota to increase SCFA production and bacteria that are beneficial to human health in a manner similar to that of the high molecular-weight forms of PHGG.

  Jul. 2023, Journal of bioscience and bioengineering, 136(1) (1), 67 - 73, English, Domestic magazine

  Scientific journal


• Metabolic engineering of the L-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae.

  Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y Hara, Tomohisa Hasunuma, Akihiko Kondo

  BACKGROUND: Glutathione is a valuable tri-peptide that is industrially produced by fermentation using the yeast Saccharomyces cerevisiae, and is widely used in the pharmaceutical, food, and cosmetic industries. It has been reported that addition of L-serine (L-Ser) is effective at increasing the intracellular glutathione content because L-Ser is the common precursor of L-cysteine (L-Cys) and glycine (Gly) which are substrates for glutathione biosynthesis. Therefore, we tried to enhance the L-Ser biosynthetic pathway in S. cerevisiae for improved glutathione production. RESULTS: The volumetric glutathione production of recombinant strains individually overexpressing SER2, SER1, SER3, and SER33 involved in L-Ser biosynthesis at 48 h cultivation was increased 1.3, 1.4, 1.9, and 1.9-fold, respectively, compared with that of the host GCI strain, which overexpresses genes involved in glutathione biosynthesis. We further examined simultaneous overexpression of SHM2 and/or CYS4 genes involved in Gly and L-Cys biosynthesis, respectively, using recombinant GCI strain overexpressing SER3 and SER33 as hosts. As a result, GCI overexpressing SER3, SHM2, and CYS4 showed the highest volumetric glutathione production (64.0 ± 4.9 mg/L) at 48 h cultivation, and this value is about 2.5-fold higher than that of the control strain. CONCLUSIONS: This study first revealed that engineering of L-Ser and Gly biosynthetic pathway are useful strategies for fermentative glutathione production by S. cerevisiase.

  Aug. 2022, Microbial cell factories, 21(1) (1), 153 - 153, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced methane production from cellulose using a two-stage process involving a bioelectrochemical system and a fixed film reactor

  Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo

  It is desirable to improve the anaerobic digestion processes of recalcitrant materials, such as cellulose. Enhancement of methane (CH₄) production from organic molecules was previously accomplished through coupling a bioelectrochemical system (BES); however, scaling-up BES-based production is difficult. Here, we developed a two-stage process consisting of a BES using low-cost and low-reactive carbon sheets as the cathode and anode, and a fixed film reactor (FFR) containing conductive material, i.e., carbon fiber textiles (CFTs) (:BES → FFR). By controlling the cathodic current at 2.7 μA/cm² without abiotic H₂ production, the three-electrode BES system was operated to mimic a microbial electrolysis cell. The thermophilic BES (inlet pH: 6.1) and FFR (inlet pH: 7.5) were operated using hydraulic retention times (HRTs) of 2.5 and 4.2 days, respectively, corresponding to a cellulose load of 3555.6 mg-carbon (C)/(L day). The BES → FFR process achieved a higher CH₄ yield (37.5%) with 52.8 vol% CH₄ in the product gas compared to the non-bioelectrochemical system (NBES) → FFR process, which showed a CH₄ yield of 22.1% with 46.8 vol% CH₄. The CH₄ production rate (67.5 mM/day) obtained with the BER → FFR process was much higher than that obtained using electrochemical methanogenesis (0.27 mM/day). Application of the electrochemical system or CFTs improved the yields of CH₄ with the NBES → FFR or BES → non-fixed film reactor process, respectively. Meta 16S rRNA sequencing revealed that putative cellulolytic bacteria (identified as Clostridium species) were present in the BES and NBES, and followed (BES→ and NBES→) FFR. Notably, H₂-consuming methanogens, Methanobacterium sp. and Methanosarcina sp., showed increased relative abundances in the suspended fraction and attached fraction of (BES→) FFR, respectively, compared to that of (NBES→) FFR, although these methanogens were observed at trace levels in the BES and NBES. These results indicate that bioelectrochemical preprocessing at a low current effectively induces interspecies H₂ transfer in the FFR with conductive material. Sufficient electrochemical preprocessing was observed using a relatively short HRT. This type of two-stage process, BES → FFR, is useful for stabilization and improvement of the biogas (CH₄) production from cellulosic material, and our results imply that the two-stage system developed here may be useful with other recalcitrant materials.

  Springer Science and Business Media LLC, Dec. 2021, Biotechnology for Biofuels, 14(1) (1)

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota

  Kengo Sasaki, Daisuke Sasaki, Katsunori Sasaki, Yuto Nishidono, Akihiro Yamamori, Ken Tanaka, Akihiko Kondo

  Daikenchuto (DKT) is a Japanese traditional herbal (Kampo) medicine containing ginseng, processed ginger, and Japanese or Chinese pepper. We aimed to determine how DKT affects human colonic microbiota. An in vitro microbiota model was established using fecal inocula collected from nine healthy volunteers, and each model was found to retain operational taxonomic units similar to the ones in the original human fecal samples. DKT was added to the in vitro microbiota model culture at a concentration of 0.5% by weight. Next-generation sequencing of bacterial 16S rRNA gene revealed a significant increase in the relative abundance of bacteria related to the Bifidobacterium genus in the model after incubation with DKT. In pure cultures, DKT significantly promoted the growth of Bifidobacterium adolescentis, but not that of Fusobacterium nucleatum or Escherichia coli. Additionally, in pure cultures, B. adolescentis transformed ginsenoside Rc to Rd, which was then probably utilized for its growth. Our study reveals the in vitro bifidogenic effect of DKT that likely contributes to its beneficial effects on the human colon.

  Springer Science and Business Media LLC, Dec. 2021, Scientific Reports, 11(1) (1), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity

  Naofumi Yoshida, Tomoya Yamashita, Tatsunori Osone, Tetsuya Hosooka, Masakazu Shinohara, Seiichi Kitahama, Kengo Sasaki, Daisuke Sasaki, Takeshi Yoneshiro, Tomohiro Suzuki, Takuo Emoto, Yoshihiro Saito, Genki Ozawa, Yushi Hirota, Yasuyuki Kitaura, Yoshiharu Shimomura, Yuko Okamatsu-Ogura, Masayuki Saito, Akihiko Kondo, Shingo Kajimura, Takeshi Inagaki, Wataru Ogawa, Takuji Yamada, Ken-ichi Hirata

  The gut microbiome has emerged as a key regulator of obesity; however, its role in brown adipose tissue (BAT) metabolism and association with obesity remain to be elucidated. We found that the levels of circulating branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) were significantly correlated with the body weight in humans and mice and that BCAA catabolic defects in BAT were associated with obesity in diet-induced obesity (DIO) mice. Pharmacological systemic enhancement of BCAA catabolic activity reduced plasma BCAA and BCKA levels and protected against obesity; these effects were reduced in BATectomized mice. DIO mice gavaged with Bacteroides dorei and Bacteroides vulgatus exhibited improved BAT BCAA catabolism and attenuated body weight gain, which were not observed in BATectomized DIO mice. Our data have highlighted a possible link between the gut microbiota and BAT BCAA catabolism and suggest that Bacteroides probiotics could be used for treating obesity.

  Elsevier BV, Nov. 2021, iScience, 24(11) (11), 103342 - 103342, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The Kobe University Human Intestinal Microbiota Model for gut intervention studies

  Namiko Hoshi, Jun Inoue, Daisuke Sasaki, Kengo Sasaki

  The human gut harbors a complex microbial community that performs a range of metabolic, physiological, and immunological functions. The host and its inhabiting microorganisms are often referred to as a "superorganism." Dysbiosis of gut microflora has been associated with the pathogenesis of intestinal disorders including inflammatory bowel disease, colorectal cancer, and extra-intestinal disorders such as cardiovascular disease. Therefore, gut microbiome interventions are important for the prevention and treatment of diseases. However, ethical, economic, scientific, and time constraints limit the outcome of human intervention or animal studies targeting gut microbiota. We recently developed an in vitro batch fermentation model (the Kobe University Human Intestinal Microbiota Model, KUHIMM) that is capable of hosting a majority of gut microbial species in humans and also detects the metabolites produced by microorganisms in real time. In this mini review, we elucidated the characteristics of the KUHIMM and its applicability in analyzing the effect of diet, drugs, probiotics, and prebiotics on intestinal bacteria. In addition, we introduce as examples its application to disease models, such as ulcerative colitis, in which intestinal bacteria are intricately involved in the process of pathogenesis. We also discuss the potential of the KUHIMM in precision medicine. KEY POINTS: • In vitro gut fermentation model to simulate human colonic microbiota • Screening of potential prebiotics and probiotic candidates in healthy model • Construction of disease models of ulcerative colitis and coronary artery disease.

  Springer Science and Business Media LLC, Apr. 2021, Applied Microbiology and Biotechnology, 105(7) (7), 2625 - 2632, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota

  Kengo Sasaki, Tomoyuki Mori, Namiko Hoshi, Daisuke Sasaki, Jun Inoue, Reiko Shinkura, Akihiko Kondo

  W27 monoclonal immunoglobulin A (IgA) suppresses pathogenic Escherichia coli cell growth; however its effect on the human intestine remains unclear. We thus aimed to determine how W27 IgA affects the human colonic microbiota using the in vitro microbiota model. This model was established using fecal samples collected from 12 healthy volunteers; after anaerobic cultivation, each model was found to retain the genera found in the original human fecal samples. After pre-incubating W27 IgA with the respective fecal sample in aerobic condition, the mixture of W27 IgA (final concentration, 0.5 µg/mL) and each fecal sample was added to the in vitro microbiota model and cultured under anaerobic condition,. Next-generation sequencing of the bacterial 16S rRNA gene revealed that W27 IgA addition significantly decreased the relative abundance of bacteria related to the genus Escherichia in the model. Additionally, at a final concentration of 5 µg/mL, W27 IgA delayed growth in pure culture of Escherichia coli isolated from human fecal samples. Our study thus revealed the suppressive effect of W27 IgA on the genus Escherichia at relatively low-concentrations and the usefulness of an in vitro microbiota model to evaluate the effect of IgA as a gut microbiota regulator.

  Research Square, Mar. 2021, English

  Link to Kobe Univ. Repository (Kernel)


• The alga Euglena gracilis stimulates Faecalibacterium in the gut and contributes to increased defecation

  Ayaka Nakashima, Kengo Sasaki, Daisuke Sasaki, Kosuke Yasuda, Kengo Suzuki, Akihiko Kondo

  The alga Euglena gracilis (E. gracilis) has recently gained attention as a health food, but its effects on human gut microbiota remain unknown. This study aimed to determine the effect of E. gracilis on gut microbiota and defecation due to modulation of microbiota composition in vitro and in vivo. The in vitro model simulating human colonic microbiota revealed that E. gracilis addition stimulated the growth of commensal Faecalibacterium. Further, E. gracilis addition enhanced butyrate production by Faecalibacterium prausnitzii. Paramylon, an insoluble dietary fibre that accumulates in E. gracilis and is the main component of E. gracilis, did not stimulate Faecalibacterium growth in vitro. Daily ingestion of 2 g of E. gracilis for 30 days increased bowel movement frequency as well as stool volume in 28 human participants. Collectively, these findings indicate that E. gracilis components other than paramylon, stimulate the growth of Faecalibacterium to improve digestive health as well as promote defecation by increasing butyrate production.

  Springer Science and Business Media LLC, Jan. 2021, Scientific Reports, 11(1) (1), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A possible beneficial effect of Bacteroides on faecal lipopolysaccharide activity and cardiovascular diseases

  Naofumi Yoshida, Tomoya Yamashita, Shigenobu Kishino, Hikaru Watanabe, Kengo Sasaki, Daisuke Sasaki, Tokiko Tabata, Yuta Sugiyama, Nahoko Kitamura, Yoshihiro Saito, Takuo Emoto, Tomohiro Hayashi, Tomoya Takahashi, Masakazu Shinohara, Ro Osawa, Akihiko Kondo, Takuji Yamada, Jun Ogawa, Ken-ichi Hirata

  Springer Science and Business Media LLC, Dec. 2020, Scientific Reports, 10(1) (1), 13009, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• In vitro human colonic microbiota utilises D-β-hydroxybutyrate to increase butyrogenesis

  Kengo Sasaki, Daisuke Sasaki, Asuka Hannya, Jun Tsubota, Akihiko Kondo

  Springer Science and Business Media LLC, Dec. 2020, Scientific Reports, 10(1) (1), 8516, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Prebiotic effects of yeast mannan, which selectively promotes Bacteroides thetaiotaomicron and Bacteroides ovatus in a human colonic microbiota model

  Shunsuke Oba, Tadahiro Sunagawa, Reiko Tanihiro, Kyoko Awashima, Hiroshi Sugiyama, Tetsuji Odani, Yasunori Nakamura, Akihiko Kondo, Daisuke Sasaki, Kengo Sasaki

  Yeast mannan (YM) is an indigestible water-soluble polysaccharide of the yeast cell wall, with a notable prebiotic effect on the intestinal microbiota. We previously reported that YM increased Bacteroides thetaiotaomicron abundance in in vitro rat faeces fermentation, concluding that its effects on human colonic microbiota should be investigated. In this study, we show the effects of YM on human colonic microbiota and its metabolites using an in vitro human faeces fermentation system. Bacterial 16S rRNA gene sequence analysis showed that YM administration did not change the microbial diversity or composition. Quantitative real-time PCR analysis revealed that YM administration significantly increased the relative abundance of Bacteroides ovatus and B. thetaiotaomicron. Moreover, a positive correlation was observed between the relative ratio (with or without YM administration) of B. thetaiotaomicron and B. ovatus (r = 0.92), suggesting that these bacteria utilise YM in a coordinated manner. In addition, YM administration increased the production of acetate, propionate, and total short-chain fatty acids. These results demonstrate the potential of YM as a novel prebiotic that selectively increases B. thetaiotaomicron and B. ovatus and improves the intestinal environment. The findings also provide insights that might be useful for the development of novel functional foods.

  Springer Science and Business Media LLC, Oct. 2020, Scientific Reports, 10(1) (1), 17351, English

  Scientific journal


• Optimal Ratio of Carbon Flux between Glycolysis and the Pentose Phosphate Pathway for Amino Acid Accumulation in Corynebacterium glutamicum.

  Katsuki Murai, Daisuke Sasaki, Shunsuke Kobayashi, Akira Yamaguchi, Hiroto Uchikura, Tomokazu Shirai, Kengo Sasaki, Akihiko Kondo, Yota Tsuge

  Glucose is metabolized through central metabolic pathways such as glycolysis and the pentose phosphate pathway (PPP) to synthesize downstream metabolites including amino acids. However, how the split ratio of carbon flux between glycolysis and PPP specifically affects the formation of downstream metabolites remains largely unclear. Here, we conducted a comprehensive metabolomic analysis to investigate the effect of the split ratio between glycolysis and the PPP on the intracellular concentration of amino acids and their derivatives in Corynebacterium glutamicum. The split ratio was varied by exchanging the promoter of a gene encoding glucose 6-phosphate isomerase (PGI). The ratio was correlated with the pgi transcription level and the enzyme activity. Concentrations of threonine and lysine-derivative 1,5-diaminopentane increased with an increase of the split ratio into the PPP. In contrast, concentrations of alanine, leucine, and valine were increased with an increase of the split ratio into glycolysis. These results could provide a new engineering target for improving the production of the amino acids and the derivatives.

  Jul. 2020, ACS synthetic biology, 9(7) (7), 1615 - 1622, English, International magazine

  [Refereed]

  Scientific journal


• Effective bifidogenic growth factors cyclo-Val-Leu and cyclo-Val-Ile produced by Bacillus subtilis C-3102 in the human colonic microbiota model

  Misaki Hatanaka, Hiroto Morita, Yumi Aoyagi, Kengo Sasaki, Daisuke Sasaki, Akihiko Kondo, Teppei Nakamura

  May 2020, Scientific Reports, 10, 7591, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Bacillus coagulans SANK 70258 suppresses Enterobacteriaceae in the microbiota of ulcerative colitis in vitro and enhances butyrogenesis in healthy microbiota.

  Kengo Sasaki, Daisuke Sasaki, Jun Inoue, Namiko Hoshi, Takayuki Maeda, Ryouichi Yamada, Akihiko Kondo

  The aim of this study was to clarify the effect of the spore-forming and lactic acid-producing probiotic strain, Bacillus coagulans SANK 70258, on human colonic microbiota of healthy subjects and ulcerative colitis patients. A model culture system was employed to construct the in vitro human colonic microbiota, to retain the bacterial species richness and simulate the patient's disordered composition, from the fecal inoculum. Bacterial 16S rRNA gene sequencing confirmed that administration of B. coagulans SANK 70258 (at an initial concentration of 4 × 107-total cells/mL) suppressed bacteria related to the family Enterobacteriaceae in the microbiota models for both healthy subjects (P = 0.016) and ulcerative colitis patients (P = 0.023). In addition, administration of B. coagulans SANK 70258 increased bacteria related to the family Lachnospiraceae (P = 0.031), thereby enhancing butyrate production (P = 0.031) in the microbiota models of healthy subjects. However, these changes were not observed in the microbiota models of ulcerative colitis patients, likely owing to the low abundance of Lachnospiraceae species. This study demonstrates the potential of B. coagulans SANK 70258 to exhibit antimicrobial activity against harmful organisms in patients with ulcerative colitis, while improving the intestinal microenvironment by increasing butyrogenesis in healthy persons. KEY POINTS: • B. coagulans SANK 70258 treatment reduced colonic Enterobacteriaceae species. • B. coagulans SANK 70258 treatment enhanced butyrogenesis in healthy individuals. • B. coagulans SANK 70258 treatment increased Lachnospiraceae in healthy persons. • B. coagulans SANK 70258 improves the colonic microenvironment in ulcerative colitis.

  Mar. 2020, Applied microbiology and biotechnology, English, International magazine

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Bifidogenic and butyrogenic effects of young barely leaf extract in an in vitro human colonic microbiota model

  Daisuke Sasaki, Kengo Sasaki, Yasushi Kadowaki, Yasuyuki Aotsuka, Akihiko Kondo

  Nov. 2019, AMB Express, 9, 182, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Butyryl-CoA:acetate CoA-transferase gene associated with the genus Roseburia is decreased in the gut microbiota of Japanese patients with ulcerative colitis

  Ryohei Shinohara, Kengo Sasaki, Jun Inoue, Namiko Hoshi, Itsuko Fukuda, Daisuke Sasaki, Akihiko Kondo, Ro Osawa

  Microbial production of butyrate is impaired in patients with ulcerative colitis (UC); however, this inhibition is not well understood in Japanese UC patients. Therefore, we quantitatively analyzed genes encoding butyryl-CoA:acetate CoA-transferase (but) and butyrate kinase (buk) in the gut microbiota of Japanese patients with UC and healthy volunteers (HVs). But showed higher levels than buk. Moreover, patients with UC showed significantly decreased levels of but associated with Roseburia sp./Eubacterium rectale compared with HVs. But, which is associated with Faecalibacterium sp., was maintained in patients with UC, with an unchanged relative abundance of Faecalibacterium sp. microorganisms in patients with UC compared with HVs.

  Oct. 2019, Bioscience of Microbiota, Food and Health, 38(4) (4), 159 - 163, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of Resistant Starch on the Gut Microbiota and Its Metabolites in Patients with Coronary Artery Disease.

  Naofumi Yoshida, Kengo Sasaki, Daisuke Sasaki, Tomoya Yamashita, Hajime Fukuda, Tomohiro Hayashi, Tokiko Tabata, Ro Osawa, Ken-Ichi Hirata, Akihiko Kondo

  AIM: Bacteroides vulgatus and B. dorei have a protective effect against atherosclerosis, suggesting that expansion of these species in the gut microbiota could help patients with coronary artery disease (CAD). This study aimed to investigate the effect of resistant starch (RS) on the gut microbiota and its metabolites in fecal sample cultures from patients with CAD and individuals without CAD, using a single-batch fermentation system. METHODS: Fecal samples from 11 patients with CAD and 10 individuals without CAD were fermented for 30 h with or without RS in the Kobe University Human Intestinal Microbiota Model (KUHIMM). Gut microbiota and the abundance of B. vulgatus and B. dorei were analyzed using 16S ribosomal ribonucleic acid (rRNA) gene sequencing and the quantitative polymerase chain reaction. Short-chain fatty acids were analyzed using high-performance liquid chromatography. RESULTS: Gut microbial analysis showed significantly lower levels of B. vulgatus and B. dorei in the original fecal samples from patients with CAD, which was simulated after 30 h of fermentation in the KUHIMM. Although RS significantly increased the absolute numbers of B. vulgatus and B. dorei, and butyrate levels in CAD fecal sample cultures, the numbers varied among each patient. CONCLUSIONS: The effect of RS on gut microbiota and its metabolites in the KUHIMM varied between CAD and non-CAD fecal sample cultures. The KUHIMM may be useful for preclinical evaluations of the effects of RS on the gut microbiota and its metabolites.

  Aug. 2019, Journal of atherosclerosis and thrombosis, 26(8) (8), 705 - 719, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Construction of a model culture system of human colonic microbiota to detect decreased Lachnospiraceae abundance and butyrogenesis in the feces of ulcerative colitis patients

  Kengo Sasaki, Jun Inoue, Daisuke Sasaki, Namiko Hoshi, Tomokazu Shirai, Itsuko Fukuda, Takeshi Azuma, Akihiko Kondo, Ro Osawa

  Compositional alteration of the gut microbiota is associated with ulcerative colitis (UC). Here, a model culture system is established for the in vitro human colonic microbiota of UC, which will be helpful for determining medical interventions. 16S ribosomal RNA sequencing confirms that UC models are successfully developed from fecal inoculum and retain the bacterial species biodiversity of UC feces. The UC models closely reproduce the microbial components and successfully preserve distinct clusters from the healthy subjects (HS), as observed in the feces. The relative abundance of bacteria belonging to the family Lachnospiraceae significantly decreases in the UC models compared to that in HS, as observed in the feces. The system detects significantly lower butyrogenesis in the UC models than that in HS, correlating with the decreased abundance of Lachnospiraceae. Interestingly, the relative abundance of Lachnospiraceae does not correlate with disease activity (defined as partial Mayo score), suggesting that Lachnospiraceae persists in UC patients at a decreased level, irrespective of the alteration in disease activity. Moreover, the system shows that administration of Clostridium butyricum MIYAIRI restores butyrogenesis in the UC model. Hence, the model detects deregulation in the intestinal environment in UC patients and may be useful for simulating the effect of probiotics.

  May 2019, Biotechnology Journal, 14(5) (5), 1800555, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae

  KOBAYASHI Jyumpei, SASAKI Daisuke, BAMBA Takahiro, HASUNUMA Tomohisa, KONDO Akihiko

  Feb. 2019, Applied Microbiology and Biotechnology, 103(3) (3), 1243 - 1254, English

  [Refereed]

  Scientific journal


• Less biomass and intracellular glutamate in anodic biofilms lead to efficient electricity generation by microbial fuel cells.

  Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Akihiko Kondo

  Background: Using a microbial fuel cell (MFC), we observed that a complex microbial community decomposed starch and transferred electrons to a graphite felt anode to generate current. In spite of the same reactor configuration, inoculum, substrate, temperature, and pH, MFCs produced different current and power density. To understand which factor(s) affected electricity generation, here, we analyzed a complex microbial community in an anodic biofilm and fermentation broth using Illumina MiSeq sequencing and metabolomics. Results: Microbial biomass on the anode was lower in MFCs generating more electricity (0.09-0.16 mg cm-2-anode) than in those generating less electricity (0.60-2.80 mg cm-2-anode), while being equal (3890-4196 mg L-1-broth) in the fermentation broth over the same operational period. Chemical oxygen demand removal and acetate concentration were also similar in fermentation broths. MFCs generating more electricity had relatively more exoelectrogenic bacteria, such as Geobacter sp., but fewer acetate-utilizing Methanosarcina sp. and/or Lactococcus sp. in anodic biofilms. Accordingly, anodic biofilms generating more electricity presented higher levels of most intracellular metabolites related to the tricarboxylic acid cycle and a higher intracellular ATP/ADP ratio, but a lower intracellular NADH/NAD+ ratio. Moreover, the level of intracellular glutamate, an essential metabolite for microbial anabolic reactions, correlated negatively with current density. Conclusion: Microbial growth on the anode and intracellular glutamate levels negatively affect electricity generation by MFCs. Reduced formation of anodic biofilm, in which intracellular glutamate concentration is 33.9 μmol g-cell-1 or less, favors the growth of acetate-utilizing Geobacter sp. on the anode and improves current generation.

  2019, Biotechnology for biofuels, 12, 72 - 72, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

  INOKUMA Kentaro, MATSUDA Mami, SASAKI Daisuke, HASUNUMA Tomohisa, KONDO Akihiko

  Sep. 2018, Microbial Cell Factories, 17, 153, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A procedure for precise determination of glutathione produced by Saccharomyces cerevisiae

  KOBAYASHI Jyumpei, SASAKI Daisuke, KONDO Akihiko

  Jun. 2018, Bio-protocol, 8(12) (12), e2887, English

  [Refereed]

  Scientific journal


• Electrochemical biotechnologies minimizing the required electrode assemblies.

  Kengo Sasaki, Daisuke Sasaki, Kazuhide Kamiya, Shuji Nakanishi, Akihiko Kondo, Souichiro Kato

  Microbial electrochemical systems (MESs) are expected to be put into practical use as an environmental technology that can support a future environmentally friendly society. However, conventional MESs present a challenge of inevitably increasing initial investment, mainly due to requirements for a large numbers of electrode assemblies. In this review, we introduce electrochemical biotechnologies that are under development and can minimize the required electrode assemblies. The novel biotechnologies, called electro-fermentation and indirect electro-stimulation, can drive specific microbial metabolism by electrochemically controlling intercellular and extracellular redox states, respectively. Other technologies, namely electric syntrophy and microbial photo-electrosynthesis, obviate the need for electrode assemblies, instead stimulating targeted reactions by using conductive particles to create new metabolic electron flows.

  Apr. 2018, Current opinion in biotechnology, 50, 182 - 188, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure

  Daisuke Sasaki, Kengo Sasaki, Naoko Ikuta, Takahiro Yasuda, FUKUDA ITSUKO, Akihiko Kondo, Ro Osawa

  Jan. 2018, Scientific Reports, 8, 435, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process.

  Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo

  Background: Bioelectrochemical systems (BESs) are an innovative technology developed to influence conventional anaerobic digestion. We examined the feasibility of applying a BES to dark hydrogen fermentation and its effects on a two-stage fermentation process comprising hydrogen and methane production. The BES used low-cost, low-reactivity carbon sheets as the cathode and anode, and the cathodic potential was controlled at - 1.0 V (vs. Ag/AgCl) with a potentiostat. The operation used 10 g/L glucose as the major carbon source. Results: The electric current density was low throughout (0.30-0.88 A/m2 per electrode corresponding to 0.5-1.5 mM/day of hydrogen production) and water electrolysis was prevented. At a hydraulic retention time of 2 days with a substrate pH of 6.5, the BES decreased gas production (hydrogen and carbon dioxide contents: 52.1 and 47.1%, respectively), compared to the non-bioelectrochemical system (NBES), although they had similar gas compositions. In addition, a methane fermenter (MF) was applied after the BES, which increased gas production (methane and carbon dioxide contents: 85.1 and 14.9%, respectively) compared to the case when the MF was applied after the NBES. Meta 16S rRNA sequencing revealed that the BES accelerated the growth of Ruminococcus sp. and Veillonellaceae sp. and decreased Clostridium sp. and Thermoanaerobacterium sp., resulting in increased propionate and ethanol generation and decreased butyrate generation; however, unknowingly, acetate generation was increased in the BES. Conclusions: The altered redox potential in the BES likely transformed the structure of the microbial consortium and metabolic pattern to increase methane production and decrease carbon dioxide production in the two-stage process. This study showed the utility of the BES to act on the microbial consortium, resulting in improved gas production from carbohydrate compounds.

  2018, Biotechnology for biofuels, 11, 173 - 173, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Transporter engineering in biomass utilization by yeast

  Kiyotaka Y. Hara, Jyumpei Kobayashi, Ryosuke Yamada, Daisuke Sasaki, Yuki Kuriya, Yoko Hirono-Hara, Jun Ishii, Michihiro Araki, Akihiko Kondo

  Nov. 2017, FEMS YEAST RESEARCH, 17(7) (7), fox061, English

  [Refereed]

  Scientific journal


• Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process.

  Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama, Akihiko Kondo

  The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.

  Aug. 2017, Applied microbiology and biotechnology, 101(15) (15), 6007 - 6014, English, International magazine

  [Refereed]

  Scientific journal


• Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae

  Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y. Hara, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2017, MICROBIAL CELL FACTORIES, 16, 44, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system.

  Kengo Sasaki, Daisuke Sasaki, Naoko Okai, Kosei Tanaka, Ryohei Nomoto, Itsuko Fukuda, Ken-Ichi Yoshida, Akihiko Kondo, Ro Osawa

  Accumulating evidence suggests that dietary taurine (2-aminoethanesulfonic acid) exerts beneficial anti-inflammatory effects in the large intestine. In this study, we investigated the possible impact of taurine on human colonic microbiota using our single-batch fermentation system (Kobe University Human Intestinal Microbiota Model; KUHIMM). Fecal samples from eight humans were individually cultivated with and without taurine in the KUHIMM. The results showed that taurine remained largely undegraded after 30 h of culturing in the absence of oxygen, although some 83% of the taurine was degraded after 30 h of culturing under aerobic conditions. Diversity in bacterial species in the cultures was analyzed by 16S rRNA gene sequencing, revealing that taurine caused no significant change in the diversity of the microbiota; both operational taxonomic unit and Shannon-Wiener index of the cultures were comparable to those of the respective source fecal samples. In addition, principal coordinate analysis indicated that taurine did not alter the composition of bacterial species, since the 16S rRNA gene profile of bacterial species in the original fecal sample was maintained in each of the cultures with and without taurine. Furthermore, metabolomic analysis revealed that taurine did not affect the composition of short-chain fatty acids produced in the cultures. These results, under these controlled but artificial conditions, suggested that the beneficial anti-inflammatory effects of dietary taurine in the large intestine are independent of the intestinal microbiota. We infer that dietary taurine may act directly in the large intestine to exert anti-inflammatory effects.

  2017, PloS one, 12(7) (7), e0180991, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparative metabolic state of microflora on the surface of the anode electrode in a microbial fuel cell operated at different pH conditions

  Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Akihiko Kondo

  Dec. 2016, AMB EXPRESS, 6, 125, English

  [Refereed]

  Scientific journal


• Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration

  Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Ayami Fujino, Daisuke Sasaki, Masahiko Morita, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

  Sep. 2016, BIORESOURCE TECHNOLOGY, 216, 830 - 837, English

  [Refereed]

  Scientific journal


• Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes

  Keisuke Yamamoto, Kiyotaka Y. Hara, Toshihiko Morita, Akira Nishimura, Daisuke Sasaki, Jun Ishii, Chiaki Ogino, Noriyuki Kizaki, Akihiko Kondo

  Sep. 2016, MICROBIAL CELL FACTORIES, 15(1) (1), 155, English

  [Refereed]

  Scientific journal


• A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics

  Risa Takagi, Kengo Sasaki, Daisuke Sasaki, Itsuko Fukuda, Kosei Tanaka, Ken-ichi Yoshida, Akihiko Kondo, Ro Osawa

  Aug. 2016, PLOS ONE, 11(8) (8), e0160533, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comprehensive metabolomic analyses of anode-respiring Geobacter sulfurreducens cells: The impact of anode-respiration activity on intracellular metabolite levels

  Jieun Song, Daisuke Sasaki, Kengo Sasaki, Souichiro Kato, Akihiko Kondo, Kazuhito Hashimoto, Shuji Nakanishi

  Jan. 2016, PROCESS BIOCHEMISTRY, 51(1) (1), 34 - 38, English

  [Refereed]

  Scientific journal


• Repeated ethanol production from sweet sorghum juice concentrated by membrane separation

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 186, 351 - 355, English

  [Refereed]

  Scientific journal


• Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content

  Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Hiroshi Teramura, Daisuke Sasaki, Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

  Jun. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, English

  [Refereed]

  Scientific journal


• Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 263 - 268, English

  [Refereed]

  Scientific journal


• Reorganization of the bacterial and archaeal populations associated with organic loading conditions in a thermophilic anaerobic digester

  Tomoyuki Hori, Shin Haruta, Daisuke Sasaki, Dai Hanajima, Yoshiyuki Ueno, Atsushi Ogata, Masaharu Ishii, Yasuo Igarashi

  Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 337 - 344, English

  [Refereed]

  Scientific journal


• Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes

  Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo

  Nov. 2014, BIORESOURCE TECHNOLOGY, 172, 83 - 90, English

  [Refereed]

  Scientific journal


• Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 380 - 386, English

  [Refereed]

  Scientific journal


• Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Satoshi Wakai, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 821 - 825, English

  [Refereed]

  Scientific journal


• Increase in lactate yield by growing Corynebacterium glutamicum in a bioelectrochemical reactor

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Akihiko Kondo

  May 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(5) (5), 598 - 601, English

  [Refereed]

  Scientific journal


• Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration

  Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Nov. 2013, BIORESOURCE TECHNOLOGY, 147, 84 - 88, English

  [Refereed]

  Scientific journal


• The membraneless bioelectrochemical reactor stimulates hydrogen fermentation by inhibiting methanogenic archaea

  Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Naoya Ohmura, Yasuo Igarashi

  Aug. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(15) (15), 7005 - 7013, English

  [Refereed]

  Scientific journal


• 電気化学システムを用いた模擬生ごみからの水素発酵の制御

  SASAKI KENGO, SASAKI DAISUKE, 森田 仁彦, 五十嵐 泰夫, KONDO AKIHIKO

  水素エネルギー協会, Mar. 2013, 水素エネルギーシステム, 38(1) (1), 33 - 37, Japanese

  Scientific journal


• Operation of a cylindrical bioelectrochemical reactor containing carbon fiber fabric for efficient methane fermentation from thickened sewage sludge

  Daisuke Sasaki, Kengo Sasaki, Atsushi Watanabe, Masahiko Morita, Norio Matsumoto, Yasuo Igarashi, Naoya Ohmura

  Elsevier Ltd, 2013, Bioresource Technology, 129, 366 - 373, English

  [Refereed]

  Scientific journal


• Efficient production of methane from artificial garbage waste by a cylindrical bioelectrochemical reactor containing carbon fiber textiles

  Daisuke Sasaki, Kengo Sasaki, Atsushi Watanabe, Masahiko Morita, Yasuo Igarashi, Naoya Ohmura

  Springer Verlag, 2013, AMB Express, 3(17) (17), 1 - 9, English

  [Refereed]

  Scientific journal


• Single-chamber bioelectrochemical hydrogen fermentation from garbage slurry

  Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi

  Oct. 2012, BIOCHEMICAL ENGINEERING JOURNAL, 68, 104 - 108, English

  [Refereed]

  Scientific journal


• Acceleration of cellulose degradation and shift of product via methanogenic co-culture of a cellulolytic bacterium with a hydrogenotrophic methanogen

  Daisuke Sasaki, Masahiko Morita, Kengo Sasaki, Atsushi Watanabe, Naoya Ohmura

  Oct. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(4) (4), 435 - 439, English

  [Refereed]

  Scientific journal


• Construction of hydrogen fermentation from garbage slurry using the membrane free bioelectrochemical system

  Kengo Sasaki, Masahiko Morita, Norio Matsumoto, Daisuke Sasaki, Shin-ichi Hirano, Naoya Ohmura, Yasuo Igarashi

  Jul. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 64 - 69, English

  [Refereed]

  Scientific journal


• Bioelectrochemical regulation accelerates facultatively syntrophic proteolysis

  Daisuke Sasaki, Kengo Sasaki, Masahiko Morita, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura

  Jul. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 59 - 63, English

  [Refereed]

  Scientific journal


• Syntrophic degradation of proteinaceous materials by the thermophilic strains Coprothermobacter proteolyticus and Methanothermobacter thermautotrophicus

  Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Jun Nagaoka, Norio Matsumoto, Naoya Ohmura, Hiraku Shinozaki

  Nov. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(5) (5), 469 - 472, English

  [Refereed]

  Scientific journal


• Detection of active, potentially acetate-oxidizing syntrophs in an anaerobic digester by flux measurement and formyltetrahydrofolate synthetase (FTHFS) expression profiling

  Tomoyuki Hori, Daisuke Sasaki, Shin Haruta, Toru Shigematsu, Yoshiyuki Ueno, Masaharu Ishii, Yasuo Lgarashi

  Jul. 2011, MICROBIOLOGY-SGM, 157, 1980 - 1989, English

  [Refereed]

  Scientific journal


• A bioelectrochemical reactor containing carbon fiber textiles enables efficient methane fermentation from garbage slurry

  Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Shin-ichi Hirano, Norio Matsumoto, Atsushi Watanabe, Naoya Ohmura, Yasuo Igarashi

  Jul. 2011, BIORESOURCE TECHNOLOGY, 102(13) (13), 6837 - 6842, English

  [Refereed]

  Scientific journal


• Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste

  Daisuke Sasaki, Tomoyuki Hori, Shin Haruta, Yoshiyuki Ueno, Masaharu Ishii, Yasuo Igarashi

  Jan. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(1) (1), 41 - 46, English

  [Refereed]

  Scientific journal


• Methanogenic communities on the electrodes of bioelectrochemical reactors without membranes

  Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi

  Jan. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(1) (1), 47 - 49, English

  [Refereed]

  Scientific journal


• Bioelectrochemical system accelerates microbial growth and degradation of filter paper

  Kengo Sasaki, Shin-ichi Hirano, Masahiko Morita, Daisuke Sasaki, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi

  Jan. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(2) (2), 449 - 455, English

  [Refereed]

  Scientific journal


• Efficient degradation of rice straw in the reactors packed by carbon fiber textiles

  Kengo Sasaki, Masahiko Morita, Shin-ichi Hirano, Daisuke Sasaki, Naoya Ohmura, Yasuo Igarashi

  Jul. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(4) (4), 1579 - 1586, English

  [Refereed]

  Scientific journal


• Efficient treatment of garbage slurry in methanogenic bioreactor packed by fibrous sponge with high porosity

  Kengo Sasaki, Daisuke Sasaki, Masahiko Morita, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi

  May 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86(5) (5), 1573 - 1583, English

  [Refereed]

  Scientific journal


• Bioelectrochemical system stabilizes methane fermentation from garbage slurry

  Kengo Sasaki, Daisuke Sasaki, Masahiko Morita, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi

  May 2010, BIORESOURCE TECHNOLOGY, 101(10) (10), 3415 - 3422, English

  [Refereed]

  Scientific journal


• Changes in bacterial community during fermentative hydrogen and acid production from organic waste by thermophilic anaerobic microflora

  Y. Ueno, D. Sasaki, H. Fukui, S. Haruta, M. Ishii, Y. Igarashi

  Aug. 2006, JOURNAL OF APPLIED MICROBIOLOGY, 101(2) (2), 331 - 343, English

  [Refereed]

  Scientific journal


• Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts

  Y Okuda, D Sasaki, S Nogami, Y Kaneko, Y Ohya, Y Anraku

  May 2003, YEAST, 20(7) (7), 563 - 573, English

  [Refereed]

  Scientific journal

• 電気化学的前処理／通電式担体充填による2段発酵を廃水・廃棄物処理へ適用

  佐々木建吾, 佐々木大介, 近藤昭彦

  May 2021, 化学と生物, 59(12) (12), 247 - 253, Japanese


• ヒト腸内細菌叢モデルによるプレバイオティクスの評価

  SASAKI DAISUKE, SASAKI KENGO

  Aug. 2019, アグリバイオ, 3(9) (9), 53 - 58, Japanese

  Introduction scientific journal


• 日本人潰瘍性大腸炎患者の腸内細菌叢における2種の酪酸産生遺伝子の定量

  篠原 涼平, 佐々木 建吾, 井上 潤, 星 奈美子, 福田 伊津子, 佐々木 大介, 近藤 昭彦, 大澤 朗

  (公財)日本ビフィズス菌センター, Apr. 2019, 腸内細菌学雑誌, 33(2) (2), 105 - 105, Japanese


• in viro培養システムによる食物繊維のヒト腸管細菌叢への影響評価

  SASAKI DAISUKE, SASAKI KENGO, KONDO AKIHIKO, OSAWA RO

  Dec. 2018, ルミナコイド研究（日本食物繊維学会誌）, 22(2) (2), 63 - 74, Japanese

  Introduction scientific journal


• 培養系ヒト腸内細菌叢モデルKUHIMMを利用した食物繊維の影響評価

  IKUTA NAOKO, 篠原 涼平, SASAKI DAISUKE, SASAKI KENGO

  May 2018, New Food Industry, 60(5) (5), 37 - 43, Japanese

  Introduction scientific journal


• 微粉砕・メタン発酵・高度膜分離による稲わらの利用

  佐々木建吾, 白井智量, 柘植陽太, 佐々木大介, 菊地淳, 近藤昭彦

  05 Mar. 2017, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.4J28a09 (WEB ONLY), Japanese


• 3P-144 Comparison of microbial metabolites between methane fermentation and acid fermentation

  Sasaki Daisuke, Sasaki Kengo, Tsuge Yota, Nakanishi Shuji, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 230 - 230, Japanese


• 1P-219 Membrane process increased ethanol production from pretreated rice straw.

  Sasaki Kengo, Tsuge Yota, Sasaki Daisuke, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 72 - 72, Japanese


• Energy recovery from agricultural wastes of plant factory by methane fermentation : Estimation of plant scale and energy balance

  渡邉 淳, 森田 仁彦, 佐々木 大介

  電力中央研究所, Apr. 2013, 電力中央研究所報告. 研究報告. 電力中央研究所環境科学研究所 編, (12011) (12011), 巻頭1 - 3,1-19, Japanese


• 1P-209 Characterization of anaerobic digestion by metabolomic analysis derived from microbial community

  SASAKI Daisuke, SASAKI Kengo, NAKANISHI Shuji, HASHIMOTO Kazuhito, KONDO Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 70 - 70, Japanese


• Increased growth of a hydrogenotrophic methanogen in co-culture with a cellulolytic bacterium under cathodic electrochemical regulation

  Daisuke Sasaki, Masahiko Morita, Kengo Sasaki, Atsushi Watanabe, Naoya Ohmura

  2013, Bioscience, Biotechnology and Biochemistry, 77(5) (5), 1096 - 1099, English

  [Refereed]

  Report scientific journal


• Utilization of Microbial Community in Microbial Conversion (Part 3) Methane Fermentation of Cellulosic Materials by the Addition of Cellulolytic Bacteria

  森田 仁彦, 佐々木 大介, 渡邉 淳

  電力中央研究所, May 2012, 電力中央研究所報告. 研究報告. 電力中央研究所環境科学研究所 編, (11038) (11038), 1 - 10,巻頭1-3, Japanese


• S19-2 Control of methanogenic microbial community using bioelectrochemical system(Session 19 Engineering tecnology in microbiology,Symposium session)

  Sasaki Kengo, Sasaki Daisuke, Morita Masahiko, Igarashi Yasuo

  日本微生物生態学会, 2012, 日本微生物生態学会講演要旨集, (28) (28), 127 - 127, English


• 4Ga12 Microbial community in the bioelectrochemical hydrogen fermenter

  Sasaki Kengo, Morita Masahiko, Sasaki Daisuke, Matsumoto Norio, Ohmura Naoya, Ishii Masaharu, Igarashi Yasuo

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 222 - 222, Japanese


• 2Ha11 Effect of facultatively syntrophic proteolysis by bioelectrochemical regulation

  SASAKI Daisuke, SASAKI Kengo, MORITA Masahiko, HIRANO Shin-ichi, MATSUMOTO Norio, OHMURA Naoya

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 71 - 71, Japanese


• 2Ha13 Methane fermentation of cellulosic materials by the addition of cellulolytic bacterium

  Morita Masahiko, Sasaki Daisuke, Watanabe Atsushi

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 71 - 71, Japanese


• Utilization of microbial community in microbial conversion (part 2) Effect of working potential in bioelectrochemical reactor on methane fermentation of cellulose materials

  渡邉 淳, 森田 仁彦, 佐々木 大介

  電力中央研究所, May 2011, 電力中央研究所報告. 研究報告. V, (10019) (10019), 1 - 9,巻頭1～3, Japanese


• 2Ca03 Analysis of bioelectrochemical packed-bed methanogenic reactor degrading sewage sludge

  Sasaki Daisuke, Sasaki Kengo, Watanabe Atsushi, Morita Masahiko, Hirano Shin-ichi, Matsumoto Norio, Uemoto Hiroaki, Ohmura Naoya, Igarashi Yasuo

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 125 - 125, Japanese


• 2Ca04 Hydrogen production from an organic solid waste using bioelectrochemical system

  Sasaki Kengo, Sasaki Daisuke, Morita Masahiko, Matsumoto Norio, Hirano Shin-ichi, Ohmura Naoya, Igarashi Yasuo

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 125 - 125, Japanese


• 2P-2031 Analyses of thermophilic bioelectrochemical methanogenic reactor including a supporting material

  SASAKI Daisuke, SASAKI Kengo, MORITA Masahiko, HIRANO Shin-ihci, MATSUMOTO Norio, OHMURA Naoya, IGARASHI Yasuo

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 114 - 114, Japanese


• 2P-2030 Ammonium tolerance in the packed-bed system

  SASAKI Kengo, MORITA Masahiko, HIRANO Shin-ichi, SASAKI Daisuke, WATAnABE Atsushi, MATSUMOTO Norioi, UEMOTO Hiroaki, OHMURA Naoya, IGARASHI Yasuo

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 114 - 114, Japanese


• P-79 Changes of gas production and microbial community by addition of ferric iron oxide or manganese oxide on the acetate degradation in thermophilic methane fermentation(Poster Session)

  YAMADA CHIHAYA, UENO YOSHIYUKI, SASAKI DAISUKE, SASAKI KENGO, ISHII MASAHARU, IGARASHI YASUO

  日本微生物生態学会, 2010, 日本微生物生態学会講演要旨集, (26) (26), 134 - 134, English


• 2Hp24 The effect of electric stimulation to the supporting material on the methanogenic packed-bed reactor

  SASAKI KENGO, MORITA MASAHIKO, HIRANO SHIN-ICHI, MATUMOTO NORIO, UEMOTO HIROAKI, OHMURA NAOYA, SASAKI DAISUKE, IGARASHI YASUO

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 114 - 114, Japanese


• 11-202 Studies on community reorganization of a methanogenic microbial community(Microbial community analysis)

  Hori Tomoyuki, Haruta Shin, Sasaki Daisuke, Ueno Yoshiyuki, Ishii Masaharu, Igarashi Yasuo

  日本微生物生態学会, 2008, 日本微生物生態学会講演要旨集, (24) (24), 159 - 159, Japanese


• 2Hp02 Analyses of bacterial community structure in a thermophilic anaerobic digester degrading organic solid wastes

  SASAKI Daisuke, HORI Tomoyuki, HARUTA Shin, UENO Yoshiyuki, ISHII Masaharu, IGARASHI Yasuo

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 198 - 198, Japanese


• 2Hp03 Molecular ecological characterization of syntrophic acetate oxidizers in a thermophilic anaerobic digester

  HORI Tomoyuki, SASAKI Daisuke, HARUTA Shin, UENO Yoshiyuki, ISHII Masaharu, IGARASHI Yasuo

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 199 - 199, Japanese


• 2Hp01 Effect of surface characteristics of support materials on packed-bed reactors

  SASAKI Kengo, MORITA Masahiko, HIRANO Shinichi, MATSUMOTO Norio, UEMOTO Hiroaki, OHMURA Naoya, SASAKI Daisuke, HARUTA Shin, IGARASHI Yasuo

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 198 - 198, Japanese


• 2A14-3 Methanogenic community structure and pathway in a thermophilic anaerobic digester degrading organic solid waste

  SASAKI Daisuke, HARUTA Shin, UENO Yoshiyuki, ISHII Masaharu, IGARASHI Yasuo

  日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 47 - 47, Japanese


• 1B16-3 Community analysis of methanogenic microflora degrading organic solid waste in continuous flow stirred tank reactor

  SASAKI Daisuke, HARUTA Shin, UENO Yoshiyuki, ISHII Masaharu, IGARASHI Yasuo

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 80 - 80, Japanese


• 2G14-1 Analysis of bacterial community structure during solubilization and hydrogen fermentation process for organic wastes

  SASAKI Daisuke, UENO Yoshiyuki, FUKUI Hisatomo, HARUTA Shin, ISHII Masaharu, IGARASHI Yasuo

  日本生物工学会, 2004, 日本生物工学会大会講演要旨集, 16, 202 - 202, Japanese

• Genetic engineering of microorganisms for high glutathione production, Glutathione: Biosynthesis, Functions and Biological Implications

  KOBAYASHI Jyumpei, SASAKI Daisuke, HARA Y. Kiyotaka, KONDO Akihiko

  Joint work, Nova Science Publishers, Feb. 2019, English

  Scholarly book

• 大麦若葉エキスがヒト大腸細菌叢に与える効果についての検討

  佐々木 建吾, 佐々木 大介, 門脇 靖司, 青塚 康幸, 近藤 昭彦

  日本農芸化学会2020年度大会


• 微生物燃料電池：アノード電極上のバイオマス量および細胞内グルタミン酸濃度と電流生成の相関

  SASAKI DAISUKE, SASAKI KENGO, TSUGE YOTA, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 解糖系とペントースリン酸経路への代謝フラックスの分岐比が細胞内代謝プロファイルに及ぼす影響

  村井 克輝, SASAKI DAISUKE, 白井 智量, 内倉 寛斗, SASAKI KENGO, 仁宮 一章, 高橋 憲司, KONDO AKIHIKO, 柘植 陽太

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• バイオ電気化学的システムによる複合微生物系の制御

  SASAKI KENGO, SASAKI DAISUKE, 森田 仁彦, TSUGE YOTA, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 冠動脈疾患患者大腸フローラモデルによる候補投与物の調査

  SASAKI KENGO, 吉田 尚史, SASAKI DAISUKE, OSAWA RO, YAMASHITA TOMOYA, 近藤 昭彦

  日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference

  Poster presentation


• E2a5ソルガムバガスの酵素糖化効率支配因子の探索

  川口秀夫, 佐々木大介, 福本妙子, 森田健太, 竹中武蔵, 冨田康平, 佐塚隆志, 松本拓也, 西野孝, 近藤昭彦

  日本農芸化学会 関西・中部支部 2019年度合同神戸大会, Sep. 2018, Japanese, Domestic conference

  Oral presentation


• 腸内細菌Bacteroides thetaiotaomicronによる食物繊維の資化性に関する研究

  篠原 涼平, 古根 隆広, 生田 直子, 佐々木 大介, 佐々木 建吾, 福田 伊津子, 大澤 朗, 坂本 憲広

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 組換え出芽酵母Saccharomyces cerevisiaeによるキシロースからのグルタチオン生産

  小林 淳平, 佐々木 大介, 番場 崇弘, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• in vitro培養系ヒト腸内細菌叢モデル（KUHIMM） を用いた難消化性食物繊維の影響評価

  SASAKI DAISUKE, SASAKI KENGO, IKUTA NAOKO, FUKUDA ITSUKO, KONDO AKIHIKO, OSAWA RO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• In vitro培養系ヒト腸内細菌叢モデルによる潰瘍性大腸炎患者の代謝プロファイル異常の検出

  SASAKI KENGO, INOUE JUN, HOSHI NAMIKO, SASAKI DAISUKE, FUKUDA ITSUKO, KONDO AKIHIKO, OSAWA RO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• In vitro潰瘍性大腸炎患者大腸フローラモデルの構築

  Kengo Sasaki, 井上 潤, 星 奈美子, Daisuke Sasaki, Akihiko Kondo, Ro Osawa

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference

  Poster presentation


• 異なる pHで運転した微生物燃料電池：アノード電極上の微生物群集に由来する細胞内代謝物の 比較

  Daisuke Sasaki, Kengo Sasaki, 柘植 陽太, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Oral presentation


• Effects of indigestible dietary fibers on human intestinal microbiota simulated in a single-batch fermentation system

  SASAKI DAISUKE, SASAKI KENGO, IKUTA NAOKO, KONDO AKIHIKO, OSAWA RO

  第21回腸内細菌学会, Jun. 2017, English, 第21回腸内細菌学会, 神戸市, Domestic conference

  Oral presentation


• 変異チオール酸化酵素による酵母の酸化型グルタチオン 生産

  小林淳平, Sasaki Daisuke, 原 清敬, Hasunuma Tomohisa, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 微粉砕・メタン発酵・高度膜分離による稲わらの利用

  Sasaki Kengo, 白井智量, Tsuge Youta, Sasaki Daisuke, 菊地 淳, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• KUHIMM による難消化性食物繊維の腸内細菌叢への影響評価

  Sasaki Daisuke, Sasaki Kengo, 生田 直子, KONDO Akihiko, 大澤 朗

  第２回デザイン生命工学研究会大会, Mar. 2017, Japanese, デザイン生命工学研究会, 神戸市, Domestic conference

  Oral presentation


• ヒト腸管モデルによるタウリンの腸内細菌叢内での動態の検証

  Sasaki Kengo, Sasaki Daisuke, KONDO Akihiko, 大澤朗

  第69回日本細菌学会関西支部総会・学術講演会, Nov. 2016, Japanese, 日本細菌学会関西支部, 大阪市, Domestic conference

  Oral presentation


• 微生物燃料電池アノード電極上の微生物群集細胞内代謝解析

  Sasaki Daisuke, Sasaki Kengo, Tsuge Youta, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• pH非調整型の培養系ヒト腸管モデルの確立

  Sasaki Kengo, Sasaki Daisuke, KONDO Akihiko, 大澤 朗

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 膜濃縮したソルガム搾汁液からのエタノール発酵

  SASAKI KENGO, TSUGE YOTA, SASAKI DAISUKE, 寺村 浩, KAWAGUCHI HIDEO, OGINO CHIAKI, 春日 重光, 佐塚 隆志, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• 稲わら前処理液の膜プロセスによるエタノール発酵高効率化

  SASAKI KENGO, TSUGE YOTA, SASAKI DAISUKE, OGINO CHIAKI, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• メタン発酵・酸発酵環境における微生物内代謝物の比較

  SASAKI DAISUKE, SASAKI KENGO, TSUGE YOTA, 中西 周次, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• Corynebacterium glutamicum増殖時の電気培養下での乳酸生成

  SASAKI KENGO, TSUGE YOTA, SASAKI DAISUKE, KONDO AKIHIKO

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference

  Oral presentation


• 微生物群集を対象とした代謝解析による嫌気消化の特徴付け

  SASAKI DAISUKE, SASAKI KENGO, 中西 周次, 橋本 和仁, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• タンパク質共生分解における電気化学的制御の影響

  SASAKI DAISUKE, Sasaki Kengo, Morita Masahiko, Hirano Shin-ichi, Matsumoto Norio, Ohmura Naoya

  日本生物工学会, Oct. 2012, Japanese, 神戸国際会議場, 【目的】我々は電気化学システムを用いた有機性廃棄物からのメタン発酵を評価し、報告してきた。これらの結果から、発酵液を還元電位に制御することによるメタン生成の安定化が明らかとなったが、その効果の由来は定かではなかった。そこで高温メタン発酵槽から分離したタンパク質分解菌と水素資化性メタン菌を用いて通電共培養を行い、通電の影響について評価し、その効果の明確化を試みた。 【方法】250mLの通電培養槽に対して、3電極方式によって－0.8V（vs. Ag/AgCl）に通電しながらスターラーで撹拌を行い、カゼインを炭素源とする培地を用いて55℃で嫌気培養を行った。通電の有り無しで共培養を行うと共に、タンパク質分解菌のみの単菌培養を対照区として計4通りの条件で培養を行い、各種パラメーターを経時的に解析した。 【結果】通電ありの共培養において菌体密度、タンパク質分解, Domestic conference

  Oral presentation

• 機能性食品の有効性をテーラーメイドに事前評価する技術基盤の創出

  佐々木 大介

  学術研究助成基金助成金／若手研究(B), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• メタン発酵による組換え微生物の有効利用に向けた導入遺伝子の動態研究

  佐々木 大介

  学術研究助成基金助成金／若手研究(B), Apr. 2015 - Mar. 2017, Principal investigator

  Competitive research funding