Research activity information

• Jun. 2024 日本生物物理学会, 第62回日本生物物理学会 若手招待講演賞, Spatiotemporal formation of a single liquid-like condensate of α-synuclein and subsequent aging by optical trapping

  柚 佳祐・茶谷G


• Jun. 2024 日本蛋白質科学会, 第24回日本蛋白質科学会年会 若手奨励賞, Spatiotemporal formation of a single liquid-like condensate of α-synuclein by optical trapping

  柚 佳祐・茶谷G


• Nov. 2023 第61回日本生物物理学会 学生発表賞

  柚 佳祐・茶谷G


• May 2022 化学とマイクロ・ナノシステム学会第45回研究会, 優秀研究賞, 液液相分離相からのアミロイド形成速度のサイズ依存性

  福山 真央,西奈美 卓,冨田 峻介,大橋 祐美子,粕谷 素洋,茶谷 絵理,丸山 洋子,白木 賢太郎,火原 彰秀


• Aug. 2020 日本生化学会, 第93回日本生化学会大会, 若手優秀発表賞

  柚 佳祐・茶谷G


• Oct. 2016 新学術領域「動的秩序と生体機能」, 第3回「動的秩序と機能」若手研究会 優秀ポスター賞, 線維前駆中間体に作用点をもつフィブリノーゲンのアミロイド線維化阻害機構

  赤井 大気, CHATANI ERI

  Japan society


• May 2016 日本生化学会近畿支部, 平成28年度日本生化学会近畿支部奨励賞, Exploring the molecular basis of self-propagation of amyloid fibrils and its manifestation through the observation of prefibrillar species

  CHATANI ERI

  Japan society


• Oct. 2015 神戸大学, 平成27年度サイエンスフロンティア研究発表会, アミロイド線維形成に与える 血液凝固因子フィブリノーゲンの影響

  Taiki Akai, Eri Chatani


• Mar. 2015 神戸大学, 神戸大学女性研究者養成システム改革加速事業総括シンポジウム 優秀ポスター賞, 一般

  CHATANI ERI

  Japan society


• Oct. 2014 神戸大学, The 5th Kobe University Brussels European Centre Symposium “AQUAPHOTOMICS: UNDERSTANDING WATER IN THE BIOLOGICAL WORLD” 優秀ポスター賞, 一般

  CHATANI ERI

  International society


• Dec. 2013 神戸大学研究基盤センター, 若手フロンティア研究会2013最優秀賞, 金属イオンによるアミロイドナノ構造の制御

  Misaki Yokoyama, Eri Chatani


• Dec. 2012 神戸大学研究基盤センター, 若手フロンティア研究会2012最優秀賞, アミロイド線維形成に及ぼすイオンの作用機構

  Yuki Masuda, Eri Chatani

• Isolation of amyloidogenic aggregates of transthyretin by ceramic hydroxyapatite chromatography

  Maria K. Tsoumpra, Shintaro Kobayashi, Eri Chatani

  Elsevier BV, May 2025, Journal of Chromatography Open, 100223 - 100223, English

  [Refereed]

  Scientific journal


• Low-complexity domains in phase-separated droplets suppress the amyloid formation of yeast prion Sup35

  Yumiko Ohhashi, Suguru Nishinami, Kentaro Shiraki, Eri Chatani, Hideki Taguchi

  Corresponding, Springer Science and Business Media LLC, Apr. 2025, npj Biosensing, 2, 13, English

  [Refereed]

  Scientific journal


• Spatiotemporal formation of a single liquid-like condensate and amyloid fibrils of α-synuclein by optical trapping at solution surface

  Keisuke Yuzu, Ching-Yang Lin, Po-Wei Yi, Chih-Hao Huang, Hiroshi Masuhara, Eri Chatani

  Liquid-like protein condensates have recently attracted much attention due to their critical roles in biological phenomena. They typically show high fluidity and reversibility for exhibiting biological functions, while occasionally serving as sites for the formation of amyloid fibrils. To comprehend the properties of protein condensates that underlie biological function and pathogenesis, it is crucial to study them at the single-condensate level; however, this is currently challenging due to a lack of applicable methods. Here, we demonstrate that optical trapping is capable of inducing the formation of a single liquid-like condensate of α-synuclein in a spatiotemporally controlled manner. The irradiation of tightly focused near-infrared laser at an air/solution interface formed a condensate under conditions coexisting with polyethylene glycol. The fluorescent dye-labeled imaging showed that the optically induced condensate has a gradient of protein concentration from the center to the edge, suggesting that it is fabricated through optical pumping-up of the α-synuclein clusters and the expansion along the interface. Furthermore, Raman spectroscopy and thioflavin T fluorescence analysis revealed that continuous laser irradiation induces structural transition of protein molecules inside the condensate to β-sheet rich structure, ultimately leading to the condensate deformation and furthermore, the formation of amyloid fibrils. These observations indicate that optical trapping is a powerful technique for examining the microscopic mechanisms of condensate appearance and growth, and furthermore, subsequent aging leading to amyloid fibril formation.

  Proceedings of the National Academy of Sciences, Sep. 2024, Proceedings of the National Academy of Sciences, 121(39) (39), e2402162121, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Fusion of amyloid beta with ferritin yields an isolated oligomeric beta-sheet-rich aggregate inside the ferritin cage.

  Basudev Maity, Shiori Kameyama, Jiaxin Tian, Thuc Toan Pham, Satoshi Abe, Eri Chatani, Kazuyoshi Murata, Takafumi Ueno

  Alzheimer's disease is a severe brain condition caused by the formation of amyloid plaques composed of amyloid beta (Aβ) peptides. These peptides form oligomers, protofibrils, and fibrils before deposition into amyloid plaques. Among these intermediates, Aβ oligomers (AβOs) were found to be the most toxic and therefore an appealing target for drug development and understanding their role in the disease. However, precise isolation and characterization of AβOs have proven challenging because AβOs tend to aggregate and form heterogeneous mixtures in solution. As a solution, we genetically fused the Aβ peptide with a ferritin monomer. Such fusion allowed the encapsulation of precisely 24 Aβ peptides inside the 24-mer ferritin cage. Using high-speed atomic force microscopy (HS-AFM), we disassembled ferritin and directly visualized the Aβ core enclosed within the cage. The thioflavin-T assay (ThT) and attenuated total reflection infrared spectroscopy (ATR-IR) revealed the presence of a β-sheet structure in the encapsulated oligomeric aggregate. Gallic acid, an amyloid inhibitor, can inhibit the fluorescence of ThT bound AβOs. Our approach represents a significant advancement in the isolation and characterization of β-sheet rich AβOs and is expected to be useful for future studies of other disordered peptides such as α-synuclein and tau.

  Apr. 2024, Biomaterials science, 12(9) (9), 2408 - 2417, English, International magazine

  [Refereed]

  Scientific journal


• Mechanistic modeling of amyloid oligomer and protofibril formation in bovine insulin

  Keisuke Yuzu, Hiroshi Imamura, Takuro Nozaki, Yuki Fujii, Shaymaa Mohamed Mohamed Badawy, Ken Morishima, Aya Okuda, Rintaro Inoue, Masaaki Sugiyama, Eri Chatani

  Elsevier BV, Mar. 2024, Journal of Molecular Biology, 436(6) (6), 168461 - 168461, English, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Oligomerization and aggregation of NAP-22 with several metal ions.

  Shohei Maekawa, Keisuke Yuzu, Eri Chatani, Kenichi Morigaki

  Metal ions participate in various biochemical processes such as electron transport chain, gene transcription, and enzymatic reactions. Furthermore, the aggregation promoting effect of several metal ions on neuronal proteins such as prion, tau, Aβ peptide, and α-synuclein, has been reported. NAP-22 (also called BASP1 or CAP-23) is a neuron-enriched calmodulin-binding protein and one of the major proteins in the detergent-resistant membrane microdomain fraction of the neuronal cell membrane. Previously, we showed oligomer formation of NAP-22 in the presence of several phospholipids and fatty acids. In this study, we found the aggregation of NAP-22 by FeCl2, FeCl3, and AlCl3 using native-PAGE. Oligomer or aggregate formation of NAP-22 by ZnCl2 or CuSO4 was shown with SDS-PAGE after cross-linking with glutaraldehyde. Morphological analysis with electron microscopy revealed the formation of large aggregates composed of small annular oligomers in the presence of FeCl3, AlCl3, or CuSO4. In case of FeCl2 or ZnCl2, instead of large aggregates, scattered annular and globular oligomers were observed. Interestingly, metal ion induced aggregation of NAP-22 was inhibited by several coenzymes such as NADP+, NADPH, or thiamine pyrophosphate. Since NAP-22 is highly expressed in the presynaptic region of the synapse, this result suggests the participation of metal ions not only on the protein and membrane dynamics at the presynaptic region, but also on the metabolic regulation though the interaction with coenzymes.

  Jan. 2024, Neuroscience letters, 821, 137623 - 137623, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Hyper Thermostability and Liquid-Crystal-Like Properties of Designed α-Helical Peptide Nanofibers.

  Minami Kurokawa, Tomoya Ohtsu, Eri Chatani, Atsuo Tamura

  Structural and thermodynamic transitions of artificially designed α-helical nanofibers were investigated using eight peptide variants, including four peptides with amide-modified carboxyl termini (CB peptides) and four unmodified peptides (CF peptides). Temperature-dependent circular dichroism spectroscopy and differential scanning calorimetry showed that CB peptides exhibit thermostability up to 50 °C higher than CF peptides. As a result, one of the denaturation temperatures approached nearly 130 °C, which is exceptionally high for a biomacromolecule. Thermodynamic analysis and microscopy observations also showed that CB peptides undergo a thermal transition similar to the phase transition in liquid crystals. In addition, one of the peptides showed a sharp and highly cooperative transition with a small enthalpy change at around 25 °C, which was ascribed to a giga-bundle burst of the molecular assembly. These macroscopic changes in the thermostability and crystallinity of CB peptides may be attributed to an increased amphiphilicity of the molecule in the direction of the helix axis, originating from the microscopic modification of the carboxyl-terminus.

  Oct. 2023, The journal of physical chemistry. B, 127(39) (39), 8331 - 8343, English, International magazine

  [Refereed]

  Scientific journal


• Variations in the Protein Hydration and Hydrogen-Bond Network of Water Molecules Induced by the Changes in the Secondary Structures of Proteins Studied through Near-Infrared Spectroscopy.

  Mika Ishigaki, Yoshiki Kato, Eri Chatani, Yukihiro Ozaki

  This study investigated how the secondary structural changes of proteins in aqueous solutions affect their hydration and the hydrogen-bond network of water molecules using near-infrared (NIR) spectroscopy. The aqueous solutions of three types of proteins, i.e., ovalbumin, β-lactoglobulin, and bovine serum albumin, were denatured by heating, and changes in the NIR bands of water reflecting the states of hydrogen bonds induced via protein secondary structural changes were investigated. On heating, the intermolecular hydrogen bonds between water molecules as well as between water and protein molecules were broken, and protein molecules were no longer strongly bound by the surrounding water molecules. Consequently, the denaturation was observed to proceed depending on the thermodynamic properties of the proteins. When the aqueous solutions of proteins were cooled after denaturation, the hydrogen-bond network was reformed. However, the state of protein hydration was changed owing to the secondary structural changes of proteins, and the variation patterns were different depending on the protein species. These changes in protein hydration may be derived from the differences in the surface charges of proteins. The elucidation of the mechanism of protein hydration and the formation of the hydrogen-bond network of water molecules will afford a comprehensive understanding of the protein functioning and dysfunctioning derived from the structural changes in proteins.

  Aug. 2023, The journal of physical chemistry. B, 127(32) (32), 7111 - 7122, English, International magazine

  [Refereed]

  Scientific journal


• Detection of fibril nucleation in micrometer-sized protein condensates and suppression of Sup35NM fibril nucleation by liquid–liquid phase separation

  Mao Fukuyama, Suguru Nishinami, Yoko Maruyama, Taiki Ozawa, Shunsuke Tomita, Yumiko Ohhashi, Motohiro Kasuya, Masao Gen, Eri Chatani, Kentaro Shiraki, Akihide Hibara

  American Chemical Society (ACS), Jun. 2023, Analytical Chemistry, 95(26) (26), 9855 - 9862, English

  [Refereed]

  Scientific journal


• Two-stage optical trapping and assembling of protein at air/solution interface

  Po-Wei Yi, Wei-Hsiang Chiu, Shuichi Toyouchi, Roger Bresoli-Obach, Johan Hofkens, Eri Chatani, Yoichiroh Hosokawa, Teruki Sugiyama, Hiroshi Masuhara

  Feb. 2023, Appl. Phys. Express, 16, 25501, English

  [Refereed]

  Scientific journal


• Tracking the Structural Development of Amyloid Precursors in the Insulin B Chain and the Inhibition Effect by Fibrinogen

  Naoki Yamamoto, Rintaro Inoue, Yoshiteru Makino, Hiroshi Sekiguchi, Naoya Shibayama, Akira Naito, Masaaki Sugiyama, Eri Chatani

  American Chemical Society (ACS), Dec. 2022, The Journal of Physical Chemistry B, 126(51) (51), 10797 - 10812, English

  [Refereed]

  Scientific journal


• Pathway dependence of the formation and development of prefibrillar aggregates in insulin B chain

  Yuki Yoshikawa, Keisuke Yuzu, Naoki Yamamoto, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Tetsushi Iwasaki, Masatomo So, Yuji Goto, Atsuo Tamura, Eri Chatani

  Amyloid fibrils have been an important subject as they are involved in the development of many amyloidoses and neurodegenerative diseases. The formation of amyloid fibrils is typically initiated by nucleation, whereas its exact mechanisms are largely unknown. With this situation, we have previously identified prefibrillar aggregates in the formation of insulin B chain amyloid fibrils, which have provided an insight into the mechanisms of protein assembly involved in nucleation. Here, we have investigated the formation of insulin B chain amyloid fibrils under different pH conditions to better understand amyloid nucleation mediated by prefibrillar aggregates. The B chain showed strong propensity to form amyloid fibrils over a wide pH range, and prefibrillar aggregates were formed under all examined conditions. In particular, different structures of amyloid fibrils were found at pH 5.2 and pH 8.7, making it possible to compare different pathways. Detailed investigations at pH 5.2 in comparison with those at pH 8.7 have suggested that the evolution of protofibril-like aggregates is a common mechanism. In addition, different processes of evolution of the prefibrillar aggregates have also been identified, suggesting that the nucleation processes diversify depending on the polymorphism of amyloid fibrils.

  MDPI AG, Jun. 2022, Molecules, 27(13) (13), 3964 - 3964, English

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Multistep growth of amyloid intermediates and its inhibition toward exploring therapeutic way: A case study using insulin B chain and fibrinogen

  Naoki Yamamoto, Eri Chatani

  Last, Biophysical Society of Japan, May 2022, Biophysics and Physicobiology, 19, e190017

  [Refereed][Invited]

  Scientific journal


• アミロイド線維の伝播に見られる構造の 保存と変化

  柚佳祐, 茶谷絵理

  Dec. 2021, C & I Commun, 46(4) (4), 19 - 21, Japanese

  [Invited]


• Cooperative optical trapping of polystyrene microparticle and protein forming a submillimeter linear assembly of microparticle

  Po-Wei Yi, Wei-Hsiang Chiu, Tetsuhiro Kudo, Teruki Sugiyama, Roger Bresoli-Obach, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Shuichi Toyouchi, Hiroshi Masuhara

  Sep. 2021, JOURNAL OF PHYSICAL CHEMISTRY C, 125(34) (34), 18988 - 18999, English

  [Refereed][Invited]

  Scientific journal


• アミロイド線維前駆中間体の観測とそれを標的とした線維形成阻害―インスリン由来モデルペプチドとフィブリノーゲンを用いた検証―

  山本 直樹, 茶谷 絵理

  Aug. 2021, 生物物理, 61(4) (4), 236 - 239, Japanese

  [Refereed][Invited]


• Current understanding of the structure, stability and dynamic properties of amyloid fibrils.

  Eri Chatani, Keisuke Yuzu, Yumiko Ohhashi, Yuji Goto

  Amyloid fibrils are supramolecular protein assemblies represented by a cross-β structure and fibrous morphology, whose structural architecture has been previously investigated. While amyloid fibrils are basically a main-chain-dominated structure consisting of a backbone of hydrogen bonds, side-chain interactions also play an important role in determining their detailed structures and physicochemical properties. In amyloid fibrils comprising short peptide segments, a steric zipper where a pair of β-sheets with side chains interdigitate tightly is found as a fundamental motif. In amyloid fibrils comprising longer polypeptides, each polypeptide chain folds into a planar structure composed of several β-strands linked by turns or loops, and the steric zippers are formed locally to stabilize the structure. Multiple segments capable of forming steric zippers are contained within a single protein molecule in many cases, and polymorphism appears as a result of the diverse regions and counterparts of the steric zippers. Furthermore, the β-solenoid structure, where the polypeptide chain folds in a solenoid shape with side chains packed inside, is recognized as another important amyloid motif. While side-chain interactions are primarily achieved by non-polar residues in disease-related amyloid fibrils, the participation of hydrophilic and charged residues is prominent in functional amyloids, which often leads to spatiotemporally controlled fibrillation, high reversibility, and the formation of labile amyloids with kinked backbone topology. Achieving precise control of the side-chain interactions within amyloid structures will open up a new horizon for designing useful amyloid-based nanomaterials.

  Apr. 2021, Int. J. Mol. Sci., 22(9) (9), English, International magazine

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Breakdown of supersaturation barrier links protein folding to amyloid formation.

  Masahiro Noji, Tatsushi Samejima, Keiichi Yamaguchi, Masatomo So, Keisuke Yuzu, Eri Chatani, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Yasushi Kawata, Kensuke Ikenaka, Hideki Mochizuki, József Kardos, Daniel E Otzen, Vittorio Bellotti, Johannes Buchner, Yuji Goto

  The thermodynamic hypothesis of protein folding, known as the "Anfinsen's dogma" states that the native structure of a protein represents a free energy minimum determined by the amino acid sequence. However, inconsistent with the Anfinsen's dogma, globular proteins can misfold to form amyloid fibrils, which are ordered aggregates associated with diseases such as Alzheimer's and Parkinson's diseases. Here, we present a general concept for the link between folding and misfolding. We tested the accessibility of the amyloid state for various proteins upon heating and agitation. Many of them showed Anfinsen-like reversible unfolding upon heating, but formed amyloid fibrils upon agitation at high temperatures. We show that folding and amyloid formation are separated by the supersaturation barrier of a protein. Its breakdown is required to shift the protein to the amyloid pathway. Thus, the breakdown of supersaturation links the Anfinsen's intramolecular folding universe and the intermolecular misfolding universe.

  Jan. 2021, Commun. Biol., 4(1) (1), 120 - 120, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Multistep changes in amyloid structure induced by cross-seeding on a rugged energy landscape.

  Keisuke Yuzu, Naoki Yamamoto, Masahiro Noji, Masatomo So, Yuji Goto, Tetsushi Iwasaki, Motonari Tsubaki, Eri Chatani

  Amyloid fibrils are aberrant protein aggregates associated with various amyloidoses and neurodegenerative diseases. It is recently indicated that structural diversity of amyloid fibrils often results in different pathological phenotypes, including cytotoxicity and infectivity. The diverse structures are predicted to propagate by seed-dependent growth, which is one of the characteristic properties of amyloid fibrils. However, much remains unknown regarding how exactly the amyloid structures are inherited to subsequent generations by seeding reaction. Here, we investigated the behaviors of self- and cross-seeding of amyloid fibrils of human and bovine insulin in terms of thioflavin T fluorescence, morphology, secondary structure, and iodine staining. Insulin amyloid fibrils exhibited different structures, depending on species, each of which replicated in self-seeding. In contrast, gradual structural changes were observed in cross-seeding, and a new type of amyloid structure with distinct morphology and cytotoxicity was formed when human insulin was seeded with bovine insulin seeds. Remarkably, iodine staining tracked changes in amyloid structure sensitively, and singular value decomposition analysis of the ultraviolet-visible absorption spectra of the fibril-bound iodine has revealed the presence of one or more intermediate metastable states during the structural changes. From these findings, we propose a propagation scheme with multistep structural changes in cross-seeding between two heterologous proteins, which is accounted for as a consequence of the rugged energy landscape of amyloid formation.

  Corresponding, Jan. 2021, Biophys. J., 120(2) (2), 284 - 295, English, International magazine

  [Refereed]

  Scientific journal


• Functional assembly of caenorhabditis elegans cytochrome b-2 (Cecytb-2) into phospholipid bilayer nanodisc with enhanced iron reductase activity.

  Hamed A Abosharaf, Yuki Sakamoto, Aliaa M Radwan, Keisuke Yuzu, Mika Fujimura, Thoria Diab, Tarek M Mohamed, Eri Chatani, Tetsunari Kimura, Motonari Tsubaki

  Among seven homologs of cytochrome b561 in a model organism C. elegans, Cecytb-2 was confirmed to be expressed in digestive organs and was considered as a homolog of human Dcytb functioning as a ferric reductase. Cecytb-2 protein was expressed in Pichia pastoris cells, purified, and reconstituted into a phospholipid bilayer nanodisc. The reconstituted Cecytb-2 in nanodisc environments was extremely stable and more reducible with ascorbate than in a detergent-micelle state. We confirmed the ferric reductase activity of Cecytb-2 by analyzing the oxidation of ferrous heme upon addition of ferric substrate under anaerobic conditions, where clear and saturable dependencies on the substrate concentrations following the Michaelis-Menten equation were observed. Further, we confirmed that the ferric substrate was converted to a ferrous state by using a nitroso-PSAP assay. Importantly, we observed that the ferric reductase activity of Cecytb-2 became enhanced in the phospholipid bilayer nanodisc.

  Jan. 2021, Biomolecules, 11(1) (1), 96, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mechanism of Nucleation in the Formation of Amyloid Fibrils

  Chatani Eri

  Amyloid fibrils generally have a cross –β structure, in which β– strands are stacked in the axial direction of the fibril. Probably because of this periodic structure, the formation of amyloid fibrils is similar to that of crystals, and the nucleation process is observed in early stages, acting as a rate–determining step. This article outlines the basic properties of amyloid fibril nucleation in the light of the nucleation theory proposed for crystallization. I also discuss how amyloid nucleation is affected at a lipid membrane interface in order to understand the mechanism of amyloid fibril formation in biological systems.

  THE MEMBRANE SOCIETY OF JAPAN, Jan. 2021, MEMBRANE, 46(1) (1), 19 - 24, Japanese

  [Refereed][Invited]


• Iodine staining as a useful probe for distinguishing insulin amyloid polymorphs.

  Takato Hiramatsu, Naoki Yamamoto, Seongmin Ha, Yuki Masuda, Mitsuru Yasuda, Mika Ishigaki, Keisuke Yuzu, Yukihiro Ozaki, Eri Chatani

  It is recently suggested that amyloid polymorphism, i.e., structural diversity of amyloid fibrils, has a deep relationship with pathology. However, its prompt recognition is almost halted due to insufficiency of analytical methods for detecting polymorphism of amyloid fibrils sensitively and quickly. Here, we propose that iodine staining, a historically known reaction that was firstly found by Virchow, can be used as a method for distinguishing amyloid polymorphs. When insulin fibrils were prepared and iodine-stained, they exhibited different colors depending on polymorphs. Each of the colors was inherited to daughter fibrils by seeding reactions. The colors were fundamentally represented as a sum of three absorption bands in visible region between 400 and 750 nm, and the bands showed different titration curves against iodine, suggesting that there are three specific iodine binding sites. The analysis of resonance Raman spectra and polarization microscope suggested that several polyiodide ions composed of I3- and/or I5- were formed on the grooves or the edges of β-sheets. It was concluded that the polyiodide species and conformations formed are sensitive to surface structure of amyloid fibrils, and the resultant differences in color will be useful for detecting polymorphism in a wide range of diagnostic samples.

  Corresponding, Oct. 2020, Sci. Rep., 10(1) (1), 16741 - 16741, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Theoretical modeling for electronic structure of polyiodide species included in α-cyclodextrin

  Masaki Okuda, Takato Hiramatsu, Mitsuru Yasuda, Mika Ishigaki, Yukihiro Ozaki, Michitoshi Hayashi, Keisuke Tominaga, Eri Chatani

  Molecular mechanism of the blue color formation in an iodine-starch reaction is studied by employing the iodine-α-cyclodextrin (α-CD) complex as a practical model system that resembles the structural properties of the blue amylose-iodine complex. To this end, we construct, using the quantum chemistry method, a molecular model of the complex (I5-/Li+/2α-CD) that consists of one I5-, two molecules of α-CD, and a lithium cation, and this model is employed as a basic unit in constructing structural models of polyiodide ion (I5-)n. The initial structure in the geometry optimization is adopted from the α-CD-iodine complex structure obtained from the X-ray crystallography study. The structural models of (I5-)n are built by adding the basic unit n times along the crystal axis and by optimizing the structure using quantum mechanics / molecular mechanics (QM (iodine) /MM (α-CD)) calculation. The electronic absorption spectra of the resulting model structures are calculated by time-dependent density functional theory (TD-DFT). We find that I5- acts as a basic unit of coloration in the visible region. The visible color originates from the electronic transition within the I5- molecule and any charge transfer between the I5- ion and either of α-CD or a coexisting counter cation are not involved. We also reveal that the electronic transitions of (I5-)n are delocalized, which accounts for the well-known observation that the color of the iodine-starch reaction becomes bluish with an increase in the chain length of amylose. Furthermore, pre-resonance Raman spectra calculated from the model suggest that the vibrational motions are localized in the I5- subunit dominantly. A comparison between an experimental absorption spectrum feature of α-CD-iodine complex and the calculated ones of (I5-)n ions with various n values suggests that (I5-)4 polyiodide ions tend to be populated dominantly in the α-CD-iodine complex under aqueous conditions.

  Corresponding, Apr. 2020, J. Phys. Chem. B, 124, 4089 - 4096, English, International magazine

  [Refereed]

  Scientific journal


• Exploration of insulin amyloid polymorphism using Raman spectroscopy and imaging

  Mika Ishigaki, Kana Morimoto, Eri Chatani, Yukihiro Ozaki

  Corresponding, Apr. 2020, Biophys. J., 118, 2997 - 3007, English

  [Refereed]

  Scientific journal


• タンパク質に対する圧力研究の新展開-基本熱力学から,分子構造に基づく理解,アミロイド線維まで

  櫻井一正, 茶谷絵理, 後藤祐児

  2020, 化学, 75(11) (11), 39 - 44


• Structural insights into the inhibition of amyloid fibril formation by fibrinogen via interaction with prefibrillar intermediates

  Naoki Yamamoto, Taiki Akai, Rintaro Inoue, Masaaki Sugiyama, TAMURA ATSUO, CHATANI ERI

  Corresponding, Jul. 2019, Biochemistry, 58(24) (24), 2769 - 2781, English

  [Refereed]

  Scientific journal


• Elucidation of molecular functions of human tumor suppressor protein 101F6 by reconstitution into phospholipid bilayer nanodiscs

  El Behery, Mohammed, Asada, Akikazu, Takeuchi, Fusako, Kimura, Tetsunari, Chatani, Eri, TSUBAKI MOTONARI

  Chemical Society of the Philippines, Jun. 2019, KIMIKA Journal of the Kapisanang Kimika ng Pilipinas, 30(1) (1), 1 - 3, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 分子夾雑のタンパク質物理化学：タンパク質凝集研究の進展とこれから

  CHATANI ERI, 後藤 祐児

  東京化学同人, May 2019, 現代化学, (578) (578), 26 - 30, Japanese

  [Invited]


• 9-Aryl-3-aminocarbazole as an environment- and stimulisensitive fluorogen and applications in lipid droplet imaging

  R. Matsubara, T. Kaiba, A. Nakata, T. Yabuta, M. Hayashi, M. Tsubaki, T. Uchino, E. Chatani

  Environment-sensitive luminophoric molecules have played an important role in the fields of smart materials, sensing, and bioimaging. In this study, it was demonstrated that depending on the substituents, 9-aryl-3-aminocarbazoles can display aggregation-induced emission and solvatofluorochromism, and the operating mechanism was clarified. The application of these compounds to lipid droplet imaging and fluorescent probes for cysteamine was demonstrated.

  Apr. 2019, J. Org. Chem., 84(9) (9), 5535 - 5547, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of temperature and hydration level on purple membrane dynamics studied using broadband dielectric spectroscopy from sub-GHz to THz regions

  Naoki Yamamoto, Shota Ito, Masahiro Nakanishi, Eri Chatani, Keiichi Inoue, Hideki Kandori, Keisuke Tominaga

  American Chemical Society, Feb. 2018, J. Phys. Chem. B, 122(4) (4), 1367 - 1377, English

  [Refereed]

  Scientific journal


• A specific form of prefibrillar aggregates that functions as a precursor of amyloid nucleation

  Naoki Yamamoto, Shoko Tsuhara, Atsuo Tamura, Eri Chatani

  Corresponding, Jan. 2018, Sci. Rep., 8, 62, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Heparin-dependent aggregation of hen egg white lysozyme reveals two distinct mechanisms of amyloid fibrillation

  Ayame Nitani, Hiroya Muta, Masayuki Adachi, Masatomo So, Kenji Sasahara, Kazumasa Sakurai, Eri Chatani, Kazumitsu Naoe, Hirotsugu Ogi, Damien Hall, Yuji Goto

  Dec. 2017, J. Biol. Chem., 292(52) (52), 21219 - 21230, English

  [Refereed]

  Scientific journal


• Recent progress on understanding the mechanisms of amyloid nucleation

  Eri Chatani, Naoki Yamamoto

  Dec. 2017, Biophys. Rev., 10, 527 - 534, English

  [Refereed][Invited]

  Scientific journal


• Femtosecond-laser-enhanced amyloid fibril formation of insulin

  Tsung-Han Liu, Ken-ichi Yuyama, Takato Hiramatsu, Naoki Yamamoto, Eri Chatani, Hiroshi Miyasaka, Teruki Sugiyama, Hiroshi Masuhara

  Corresponding, Aug. 2017, Langmuir, 33(33) (33), 8311 - 8318, English

  [Refereed]

  Scientific journal


• Early aggregation preceding the nucleation of insulin amyloid fibrils as monitored by small angle X-ray scattering

  Eri Chatani, Rintaro Inoue, Hiroshi Imamura, Masaaki Sugiyama, Minoru Kato, Masahide Yamamoto, Koji Nishida, Toshiji Kanaya

  Oct. 2015, Sci. Rep., 5, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• プログラムされていないフォールディング－アミロイド線維の形成－

  茶谷 絵理

  日本生化学会, Jun. 2015, 生化学, 87(3) (3), 292 - 297, Japanese

  [Refereed][Invited]


• 小角X線散乱によるアミロイド線維形成機構に関する研究

  井上 倫太郎, 茶谷 絵理, 金谷 利治

  2015, SPring-8/SACLA利用研究成果集, 3, 2011B1996/BL40B2, Japanese

  [Refereed]

  Research institution


• Controlling Nanostructures Of Insulin Amyloid Fibrils Using Metal Ions

  Misaki Yokoyama, Yoshito Furuie, Motonari Tsubaki, Hiroshi Hori, Takamasa Nishida, Kazuo Eda, Eri Chatani

  Jul. 2014, PROTEIN SCIENCE, 23, 245 - 245, English


• Near Infrared Spectral Monitoring Reveals Water Molecular System Dynamics During The Amyloidogenic Nucleation

  Eri Chatani, Yutaro Tsuchisaka, Yuuki Masuda, Roumiana Tsenkova

  Jul. 2014, PROTEIN SCIENCE, 23, 87 - 87, English


• Water molecular system eynamics associated with amyloidogenic nucleation as revealed by real time near infrared spectroscopy and Aquaphotomics

  Eri Chatani, Yutaro Tsuchisaka, Yuki Masuda, Roumiana Tsenkova

  Jul. 2014, PLoS One, 9(7) (7), e101997, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Stepwise Organization of the beta- Structure Identifies Key Regions Essential for the Propagation and Cytotoxicity of Insulin Amyloid Fibrils

  Eri Chatani, Hiroshi Imamura, Naoki Yamamoto, Minoru Kato

  Apr. 2014, JOURNAL OF BIOLOGICAL CHEMISTRY, 289(15) (15), 10399 - 10410, English

  [Refereed]

  Scientific journal


• 超小角X線散乱によるインスリンアミロイド線維の構造解析

  茶谷 絵理, 井上 倫太郎, 竹中 幹人, 西田 幸次, 金谷 利治

  Feb. 2013, SPring-8/SACLA利用研究成果集, 1, 2011B1951, Japanese

  [Refereed]


• Polymorphism of beta(2)-Microglobulin Amyloid Fibrils Manifested by Ultrasonication-enhanced Fibril Formation in Trifluoroethanol

  Eri Chatani, Hisashi Yagi, Hironobu Naiki, Yuji Goto

  Jun. 2012, JOURNAL OF BIOLOGICAL CHEMISTRY, 287(27) (27), 22827 - 22837, English

  [Refereed]

  Scientific journal


• アミロイド線維伸長における中間体構造の捕捉と構造解析

  CHATANI Eri, 小沼 剛, 後藤 祐児

  The Biophysical Society of Japan General Incorporated Association, May 2012, 生物物理, 52(3) (3), 148 - 149, Japanese

  [Refereed][Invited]


• Kinetic Intermediates of beta(2)-Microglobulin Fibril Elongation Probed by Pulse-Labeling H/D Exchange Combined with NMR Analysis

  Tsuyoshi Konuma, Eri Chatani, Masanori Yagi, Kazumasa Sakurai, Takahisa Ikegami, Hironobu Naiki, Yuji Goto

  Jan. 2011, JOURNAL OF MOLECULAR BIOLOGY, 405(3) (3), 851 - 862, English

  [Refereed]

  Scientific journal


• Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy

  Yuichi Yoshimura, Kazumasa Sakurai, Young-Ho Lee, Takahisa Ikegami, Eri Chatani, Hironobu Naiki, Yuji Goto

  Dec. 2010, PROTEIN SCIENCE, 19(12) (12), 2347 - 2355, English

  [Refereed]

  Scientific journal


• Pre-Steady-State Kinetic Analysis of the Elongation of Amyloid Fibrils of beta(2)-Microglobulin with Tryptophan Mutagenesis

  Eri Chatani, Reina Ohnishi, Tsuyoshi Konuma, Kazumasa Sakurai, Hironobu Naiki, Yuji Goto

  Lead, Jul. 2010, JOURNAL OF MOLECULAR BIOLOGY, 400(5) (5), 1057 - 1066, English

  [Refereed]

  Scientific journal


• FTIR observation of compression recovery of the secondary structure of heat denaturated ribonuclease A in sucrose solution

  Tsubasa Yamamoto, Eri Chatani, Minoru Kato

  Mar. 2010, J. Phys.: Conf. Ser., 215, 012155, English

  [Invited]

  International conference proceedings


• Ultrasonication-dependent production and breakdown lead to minimum-sized amyloid fibrils

  Eri Chatani, Young-Ho Lee, Hisashi Yagi, Yuichi Yoshimura, Hironobu Naiki, Yuji Goto

  Lead, Jul. 2009, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(27) (27), 11119 - 11124, English

  [Refereed]

  Scientific journal


• A Comprehensive Model for Packing and Hydration for Amyloid Fibrils of beta(2)-Microglobulin

  Young-Ho Lee, Eri Chatani, Kenji Sasahara, Hironobu Naiki, Yuji Goto

  Jan. 2009, JOURNAL OF BIOLOGICAL CHEMISTRY, 284(4) (4), 2169 - 2175, English

  [Refereed]

  Scientific journal


• Kinetic Coupling of Folding and Prolyl Isomerization of beta(2)-Microglobulin Studied by Mutational Analysis

  Michiko Sakata, Eri Chatani, Atsushi Kameda, Kazumasa Sakurai, Hironobu Naiki, Yuji Goto

  Lead, Oct. 2008, JOURNAL OF MOLECULAR BIOLOGY, 382(5) (5), 1242 - 1255, English

  [Refereed]

  Scientific journal


• Structure, Formation and Propagation of Amyloid Fibrils

  Yuji Goto, Hisashi Yagi, Keiichi Yamaguchi, Eri Chatani, Tadato Ban

  2008, CURRENT PHARMACEUTICAL DESIGN, 14(30) (30), 3205 - 3218, English

  [Refereed]

  Scientific journal


• Seed-dependent accelerated fibrillation of alpha-synuclein induced by periodic ultrasonication treatment

  Hyun Jin Kim, Eri Chatani, Yuji Goto, Seung R. Paik

  Dec. 2007, JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 17(12) (12), 2027 - 2032, English

  [Refereed]

  Scientific journal


• Recent applications of high pressure to the studies of amyloid fibrils

  Eri Chatani, Yuji Goto

  Japan Society of High Pressure Science and Technology, 2007, Review of High Pressure Science and Technology/Koatsuryoku No Kagaku To Gijutsu, 17(1) (1), 42 - 49, Japanese


• Conformation of amyloid fibrils of beta(2)-microglobulin probed by tryptophan mutagenesis

  Miho Kihara, Eri Chatani, Kentaro Iwata, Kaori Yamamoto, Takanori Matsuura, Atsushi Nakagawa, Hironobu Naiki, Yuji Goto

  Oct. 2006, JOURNAL OF BIOLOGICAL CHEMISTRY, 281(41) (41), 31061 - 31069, English

  [Refereed]

  Scientific journal


• Seeding-dependent propagation and maturation of beta(2)-microglobulin amyloid fibrils under high pressure

  Eri Chatani, Hironobu Naiki, Yuji Goto

  Lead, Jun. 2006, JOURNAL OF MOLECULAR BIOLOGY, 359(4) (4), 1086 - 1096, English

  [Refereed]

  Scientific journal


• Structural stability of amyloid fibrils of beta(2)-microglobulin in comparison with its native fold

  E Chatani, Y Goto

  Lead, Nov. 2005, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1753(1) (1), 64 - 75, English

  [Refereed]

  Scientific journal


• Main-chain dominated amyloid structures demonstrated by the effect of high pressure

  E Chatani, M Kato, T Kawai, H Naiki, Y Goto

  Lead, Sep. 2005, JOURNAL OF MOLECULAR BIOLOGY, 352(4) (4), 941 - 951, English

  [Refereed]

  Scientific journal


• Seeding-dependent maturation of ss(2)-microglobulin amyloid fibrils at neutral pH

  M Kihara, E Chatani, M Sakai, K Hasegawa, H Naiki, Y Goto

  Mar. 2005, JOURNAL OF BIOLOGICAL CHEMISTRY, 280(12) (12), 12012 - 12018, English

  [Refereed]

  Scientific journal


• Critical balance of electrostatic and hydrophobic interactions is required for beta(2)-microglobulin amyloid fibril growth and stability

  B Raman, E Chatani, M Kihara, T Ban, M Sakai, K Hasegawa, H Naiki, CM Rao, Y Goto

  Feb. 2005, BIOCHEMISTRY, 44(4) (4), 1288 - 1299, English

  [Refereed]

  Scientific journal


• アミロイド線維形成機構−裏の世界の構築原理

  茶谷 絵理, 後藤 祐児

  2005, 実験医学, 23, 2384 - 2390


• Conformational stability of amyloid fibrils of beta-microglobulin probed by guanidine-hydrochloride-induced unfolding

  T Narimoto, K Sakurai, A Okamoto, E Chatani, M Hoshino, K Hasegawa, H Naiki, Y Goto, Structural and functional changes in bovine pancreatic ribonuclease A by the replacement of Phe120 with other hydrophobic residues

  Oct. 2004, FEBS LETTERS, 576(3) (3), 313 - 319, English

  [Refereed]

  Scientific journal


• Indiscriminate glycosylation of procarboxypeptidase Y expressed in Pichia pastoris

  Haruko Maeda, Eri Chatani, Takahiro Koyama, Masatoshi Sugiura, Hirohisa Izumi, Rikimaru Hayashi

  Corresponding, Apr. 2004, Carbohydr. Res., 339(6) (6), 1041 - 1045, English

  [Refereed]

  Scientific journal


• Application of pressure to the analysis of hydrophobic core of protein : Study of bovine pancreatic ribonuclease A and the mutant enzymes

  CHATANI Eri

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2004, Nippon Nōgeikagaku Kaishi, 78(4) (4), 407 - 409, Japanese


• Minimization of cavity size ensures protein stability and folding: Structures of Phe46-replaced bovine pancreatic RNase A

  Tetsuya Kadonosono, Eri Chatani, Rikimaru Hayashi, Hideaki Moriyama, Tatsuo Ueki

  Sep. 2003, Biochemistry, 42(36) (36), 10651 - 10658, English

  [Refereed]

  Scientific journal


• Comparison of heat- and pressure-induced unfolding of ribonuclease A: The critical role of Phe46 which appears to belong to a new hydrophobic chain-folding initiation site

  Eri Chatani, Kazuhiko Nonomura, Rikimaru Hayashi, Claude Balny, Reinhard Lange

  Lead, Apr. 2002, Biochemistry, 41(14) (14), 4567 - 4574, English

  [Refereed]

  Scientific journal


• Thermal and pressure stability of Phe46 mutants of ribonuclease A

  E Chatani, R Hayashi, R Lange, C Balny

  Lead, Jan. 2002, Trends in High Pressure Bioscience and Biotechnology, 19, 27 - 32, English

  International conference proceedings


• Conformational strictness required for maximum activity and stability of bovine pancreatic ribonuclease A as revealed by crystallographic study of three Phe 120 mutants at 1.4 angstrom resolution

  Eri Chatani, Rikimaru Hayashi, Hideaki Moriyama, Tatsuo Ueki

  Lead, Jan. 2002, Protein Sci., 11(1) (1), 72 - 81, English

  [Refereed]

  Scientific journal


• (博士取得論文)The structural basis for the functionality and stability of bovine pancreatic ribonuclease A

  2002


• The structural basis for functionality and stability of hydrophobic core in RNase A as probed by the application of high pressure

  Eri Chatani, Tetsuya Kadonosono, Rikimaru Hayashi

  Lead, 2002, Advances in High Pressure Bioscience and Biotechnology II, 87 - 94


• Functional and structural roles of constituent amino acid residues of bovine pancreatic ribonuclease A

  Eri Chatani, Rikimaru Hayashi

  Lead, Society of Fermentation and Bioengineering, Aug. 2001, Journal of Bioscience and Bioengineering, 92(2) (2), 98 - 107, English

  [Refereed]

  Scientific journal


• Expression of soluble bovine pancreatic ribonuclease A in Pichia pastoris and its purification and characterization

  Eri Chatani, Naoki Tanimizu, Hiroshi Ueno, Rikimaru Hayashi

  Lead, Nov. 2000, Biosci. Biotech. Biochem., 64(11) (11), 2437 - 2444, English

  [Refereed]

  Scientific journal


• Structural and functional changes in bovine pancreatic ribonuclease A by the replacement of Phe120 with other hydrophobic residues

  Eri Chatani, Naoki Tanimizu, Hiroshi Ueno, Rikimaru Hayashi

  Lead, Jun. 2000, J. Biochem., 129(6) (6), 917 - 922, English

  [Refereed]

  Scientific journal

• ウシ由来インスリンのアミロイド核形成に見られるオリゴマーおよびプロトフィブリル形成機構

  柚佳祐, 今村比呂志, 野崎拓郎, 藤井悠生, 守島健, 奥田綾, 井上倫太郎, 杉山正明, 茶谷絵理

  2023, 日本蛋白質科学会年会プログラム・要旨集, 23rd (CD-ROM)


• トランスサイレチン49-127断片のアミロイド線維形成反応における野生型と変異体の比較

  中尾星哉, 柚佳祐, 松村美里, 大橋祐美子, 茶谷絵理

  2023, 日本蛋白質科学会年会プログラム・要旨集, 23rd (CD-ROM)


• ドデシル硫酸ナトリウム共存下でのαシヌクレイン凝集反応の多様性

  北野さくら, 柚佳祐, 笹田航, 木村哲就, 茶谷絵理

  2023, 日本蛋白質科学会年会プログラム・要旨集, 23rd (CD-ROM)


• Development of a microfluidic device for long-term observation of protein condensates for amyloid nucleation analysis

  小澤大樹, 小澤大樹, 西奈美卓, 冨田峻介, 大橋祐美子, 粕谷素洋, 茶谷絵理, 丸山洋子, 白木賢太郎, 火原彰秀, 火原彰秀, 火原彰秀, 福山真央, 福山真央, 福山真央

  2023, 日本化学会春季年会講演予稿集(Web), 103rd


• 酸化状態に見られるタウ液滴の経時変化

  道上佑希, 益井歩未, 大橋祐美子, 柚佳祐, 山口圭一, 河田康志, 茶谷絵理

  2023, 日本蛋白質科学会年会プログラム・要旨集, 23rd (CD-ROM)


• Analysis of aggregation mechanism of tau droplets in oxidized state

  道上佑希, 大橋祐美子, 大橋祐美子, 益井歩未, 柚佳祐, 山口圭一, 河田康志, 茶谷絵理

  2023, 日本分子生物学会年会プログラム・要旨集(Web), 46th


• 酵母プリオンタンパク質を用いた液液相分離のアミロイド核生成の影響評価

  福山真央, 西奈美卓, 冨田峻介, 大橋祐美子, 大橋祐美子, 小澤大樹, 玄大雄, 粕谷素洋, 茶谷絵理, 丸山洋子, 白木賢太郎, 火原彰秀

  2023, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 47th


• Understanding protein droplets as a site of amyloid fibril formation

  茶谷絵理, 大橋祐美子, 大橋祐美子, 福山真央

  2023, 日本分子生物学会年会プログラム・要旨集(Web), 46th


• A local structure of the intrinsically disordered region of Sup35 governs the temperature sensitivity of liquid-liquid phase separation.

  大橋祐美子, 大橋祐美子, 西奈美卓, 白木賢太郎, 茶谷絵理

  2023, 日本分子生物学会年会プログラム・要旨集(Web), 46th


• SAXS analysis for elucidating the inhibitory mechanism of the amyloid precursor of insulin B-chain through the interaction with αB-crystallin

  Y. Kokuo, K. Yuzu, N. Yamamoto, K. Morishima, A. Okuda, R. Inoue, M. Sugiyama, J. Hayashi, J. A. Carver, E. Chatani

  Corresponding, 2022, KURNS Progress Report, English

  Introduction research institution


• 天然変性領域の局所構造がSup35の液-液相分離の環境応答性を決定する

  大橋祐美子, 西奈美卓, 白木賢太郎, 茶谷絵理

  2022, 日本蛋白質科学会年会プログラム・要旨集, 22nd (Web)


• アミロイド線維前駆中間体の構造発達とその阻害-インスリンB鎖とフィブリノーゲンを用いた研究例-

  山本直樹, 茶谷絵理

  2022, 日本蛋白質科学会年会プログラム・要旨集, 22nd (Web)


• Size dependence of the amyloid nucleation in protein condensates formed by liquid-liquid phase separation

  福山真央, 福山真央, 西奈美卓, 冨田峻介, 大橋祐美子, 粕谷素洋, 茶谷絵理, 丸山洋子, 白木賢太郎, 火原彰秀, 火原彰秀

  2022, 化学とマイクロ・ナノシステム, 21(2) (2)


• プリオンタンパク質濃縮相内アミロイド核生成の速度論的解析に向けた溶媒条件検討

  小澤大樹, 小澤大樹, 西奈美卓, 冨田峻介, 大橋祐美子, 粕谷素洋, 茶谷絵里, 丸山洋子, 白木賢太郎, 火原彰秀, 福山真央, 火原彰秀, 福山真央, 火原彰秀, 福山真央

  2022, 東北大学多元物質科学研究所研究発表会講演予稿集, 22nd


• 液液相分離相からのアミロイド形成速度のサイズ依存

  福山真央, 福山真央, 西奈美卓, 冨田峻介, 大橋祐美子, 粕谷素洋, 茶谷絵理, 丸山洋子, 白木賢太郎, 火原彰秀, 火原彰秀

  2022, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 45th


• 近赤外分光法を用いた,タンパク質の2次構造変化に伴う水分子の水素結合ネットワークの変化についての分析

  石垣美歌, 加藤慎基, 茶谷絵理

  2022, 日本分析化学会年会講演要旨集(Web), 71st


• マイクロサイズプリオンタンパク質集合液滴中からのアミロイド核生成速度解析

  福山真央, 西奈美卓, 冨田峻介, 大橋祐美子, 粕谷素洋, 茶谷絵理, 白木賢太郎, 火原彰秀, 火原彰秀

  2022, 分析化学討論会講演要旨集(Web), 82nd


• Analysis of distinct amyloid formation pathways between bovine and human insulin

  Keisuke Yuzu, Takuro Nozaki, Ken Morishima, Aya Okuda, Rintaro Inoue, Masaaki Sugiyama, Eri Chatani

  Corresponding, 2021, KURNS Progress Report, English


• 線維前駆中間体に作用するαB-クリスタリンのアミロイド線維形成阻害機構

  國尾祐貴, 柚佳祐, CARVER John A., 茶谷絵理

  2021, 日本生化学会大会(Web), 94th


• 動的光散乱法によるタウのオリゴマー形成機構の解析

  益井歩未, 柚佳祐, 山口圭一, 後藤祐児, 河田康志, 茶谷絵理

  2021, 日本生化学会大会(Web), 94th


• Understanding protein aggregation towards amyloid fibrils

  茶谷絵理

  2021, 日本分子生物学会年会プログラム・要旨集(Web), 44th


• 超音波装置HANABIを用いたアミロイド関連疾患の超早期診断法の樹立

  池中建介, AGUIRRE Cesar, 茶谷絵理, 後藤祐児, 望月秀樹

  2021, 日本アミロイドーシス学会学術集会プログラム・抄録集, 8th


• SAXS analysis of the formation process of the nucleation intermediate of insulin B chain: The measurement at different pH conditions

  Yuki Yoshikawa, Naoki Yamamoto, Rintaro Inoue, Masaaki Sugiyama, Eri Chatani

  Corresponding, 2020, KURNS Progress Report, English

  Introduction research institution


• X線小角散乱によるアミロイド線維前駆中間体発達の時分割追跡

  山本直樹, 赤井大気, 井上倫太郎, 杉山正明, 柴山修哉, 茶谷絵理

  2020, KURNS-EKR (Web), (6) (6)


• アミロイド核形成メカニズムの解明を目指した核形成中間体の捕捉と構造解析

  吉川祐樹, 山本直樹, 井上倫太郎, 守島健, 杉山正明, 田村厚夫, 茶谷絵理

  2020, KURNS-EKR (Web), (6) (6)


• Small angle X-ray scattering measurements on insulin B-chain nucleation intermediate complexed with fibrinogen

  Naoki Yamamoto, Eri Chatani, Taiki Akai, Rintaro Inoue, Masaaki Sugiyama

  Corresponding, 2019, KURNS Progress Report, English

  Introduction research institution


• フィブリノーゲンによるインスリンB鎖アミロイド線維の形成阻害

  茶谷絵理, 山本直樹, 赤井大気, 井上倫太郎, 杉山正明, 田村厚夫

  2019, KURNS-EKR (Web), (3) (3)


• Time resolved small angle X-ray scattering (SAXS) measurements on the formation of insulin B chain prefibrillar intermediates

  Naoki Yamamoto, Eri Chatani, Rintaro Inoue, Ken Morishima, Masaaki Sugiyama

  2018, KURNS Progress Report, English

  Introduction research institution


• SAXS study on the structure of prefibrillar intermediates for the formation of insulin B chain amyloid fibrils

  Naoki Yamamoto, Eri Chatani, Taiki Akai, Rintaro Inoue, Masaaki Sugiyama

  Corresponding, 2017, KURRI Progress Report, English


• アミロイド伝播核の形成メカニズムの解明

  茶谷絵理

  2015, KURRI-KR, (203) (203)


• 3P-010 Analysis of the amyloid fiber extension mechanism using H/D exchange(The 46th Annual Meeting of the Biophysical Society of Japan)

  Yanagi Kotaro, Sakurai Kazumasa, Chatani Eri, Naiki Hironobu, Goto Yuji

  The Biophysical Society of Japan General Incorporated Association, 2008, Seibutsu Butsuri, 48, S129, English


• 2P-083 Monitoring the fibrillation intermediate of β2-microglobulin by Trp fluorescence and H/D exchange-NMR(The 46th Annual Meeting of the Biophysical Society of Japan)

  Chatani Eri, Onishi Reina, Konuma Tsuyoshi, Sakurai Kazumasa, Naiki Hironobu, Goto Yuji

  The Biophysical Society of Japan General Incorporated Association, 2008, Seibutsu Butsuri, 48, S88, English


• 2P-008 Structural Analysis of Amyloid Fibrils of β2-Microglobulin by Solid-State NMR(The 46th Annual Meeting of the Biophysical Society of Japan)

  Saeka Miwako, Todokoro Yasuto, Egawa Ayako, Kameda Atsushi, Chatani Eri, Yagi Hisashi, Akutsu Hideo, Naiki Hironobu, Fujiwara Toshimichi, Goto Yuji

  The Biophysical Society of Japan General Incorporated Association, 2008, Seibutsu Butsuri, 48, S76, English


• 3P055 Uniforming the Molecular Weigh of Amyloid Fibrils by Ultrasonication(Proteins-stability, folding, and other physicochemical properties,Oral Presentations)

  Chatani Eri, Adachi Rumi, Sakai Miyo, Naiki Hironobu, Goto Yuji

  The Biophysical Society of Japan General Incorporated Association, 2007, Seibutsu Butsuri, 47, S216, English


• 2P109 Folding and Unfolding Kinetics of β2-Microglobulin as Probed by Tryptophan Fluorescence(31. Protein folding and misfolding (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Sakata Michiko, Chatani Eri, Kameda Atsushi, Sakurai Kazumasa, Naiki Hironobu, Goto Yuji

  The Biophysical Society of Japan General Incorporated Association, 2006, Seibutsu Butsuri, 46(2) (2), S323, English


• 3P046 Structural analysis of amyloid fibrils ofβ2-microglobulin using tryptophan fluorescence probe

  Kihara M., Yamamoto K., Iwata K., Chatani E., Hasegawa K., Naiki H., Goto Y.

  The Biophysical Society of Japan General Incorporated Association, 2005, Seibutsu Butsuri, 45, S215, Japanese

• 高圧力下の生物科学

  金品, 昌志, 田村, 勝弘, 林, 力丸

  圧力で探るアミロイド線維の構造構築原理 / 茶谷絵理, 内木宏延, 後藤祐児 著, さんえい出版, Nov. 2006, Japanese, ISBN: 4915592812

• Nonlinearly evolving dynamics of optical trapping-induced phase separation of protein at solution surface

  Hiroshi Masuhara, Keisuke Yuzu, Yi-Sian Chien, Eri Chatani

  Focus on Microscopy 2025, Apr. 2025, English, 台北, International conference

  Oral presentation


• 液滴を介して進行するタンパク質異常凝集のメカニズム

  茶谷絵理

  第7回三融会・武田神経科学シンポジウム, Apr. 2025, Japanese, 東京, Domestic conference

  [Invited]

  Invited oral presentation


• マイクロ水滴を用いたアルファシヌクレイン凝集体形成の観察

  佐藤 安里, 柚 佳祐, 藤塚 健次, 茶谷 絵理, 丸山 洋子, 火原 彰秀, 福山 真央

  日本化学会第105春季年会, Mar. 2025, Japanese, 千葉, Domestic conference

  Poster presentation


• Selective Formation of a Single Droplet and Aggregate of Protein Lysozyme by Optical Trapping at Solution Surface

  Yi-Sian Chien, Keisuke Yuzu, Shuichi Toyouchi, Henryk Witek, Eri Chatani, Hiroshi Masuhara

  第72回応用物理学会春季学術講演会, Mar. 2025, English, 千葉, Domestic conference

  Poster presentation


• 金ナノ粒子結合による溶液表面における光捕捉誘起αシヌクレイン液滴形成の促進

  柚佳祐, 茶谷絵理, 増原宏

  第72回応用物理学会春季学術講演会, Mar. 2025, Japanese, 千葉, Domestic conference

  Oral presentation


• レーザートラッピングを利用したタンパク質液滴の形成制御

  茶谷絵理、柚佳祐、Ching-Yang Lin、Po-Wei Yi、Chih-Hao Huang、増原宏

  神戸大学第7回広帯域極限電磁波生命理工連携研究会, Jan. 2025, Japanese, 神戸, Domestic conference

  [Invited]

  Oral presentation


• 酸化ストレスがタウ液滴の凝集に及ぼす影響の解析

  道上佑希, 益井歩未, 大橋祐美子, 柚佳祐, 岩崎哲史, 山口圭一, 河田康志, 茶谷絵理

  神戸大学研究基盤センター若手フロンティア研究会2024, Dec. 2024, Japanese, 神戸, Domestic conference

  Poster presentation


• αB-クリスタリンによる αシヌクレイン液滴の老化の阻害

  藤塚健次, 柚佳祐, 道上祐希, John A. Carve, 茶谷絵理

  神戸大学研究基盤センター若手フロンティア研究会2024, Dec. 2024, Japanese, 神戸, Domestic conference

  Poster presentation


• アルファシヌクレイン濃縮相からのアミロイド核生成解析に向けた濃縮相の長時間測定

  佐藤安里, 柚佳祐, 藤塚健次, 茶谷絵理, 丸山洋子, 火原彰秀, 福山真央

  化学とマイクロ・ナノシステム学会第50回研究会, Nov. 2024, Japanese, 仙台, Domestic conference

  Poster presentation


• Optical trapping-induced spatiotemporal formation of a single liquid-like condensate of α-synuclein at solution surface

  Keisuke Yuzu, Ching-Yang Lin, Chih-Hao Huang, Po-Wei Yi, Hiroshi Masuhara, Eri Chatani

  大阪大学蛋白質研究所セミナー・生物物理サブグループ・LLPS研究会合同セミナー 「Toward establishing LLPS research systems」, Sep. 2024, English, 大阪, International conference

  [Invited]

  Invited oral presentation


• 溶液表面における光圧捕捉によるαシヌクレインの単一液滴の時空間的形成

  柚佳祐, Ching-Yang Lin, Po-Wei Yi, Chih-Hao Huang, 茶谷絵理, 増原宏

  第85回応用物理学会秋季学術講演会, Sep. 2024, Japanese, 福岡, Domestic conference

  Oral presentation


• 溶液表面におけるαシヌクレインのoptically evolved assemblingによる単一液滴の時空間的形成

  柚佳祐, Ching-Yang Lin, Po-Wei Yi, Chih-Hao Huang, 茶谷絵理, 増原宏

  2024年光化学討論会, Sep. 2024, Japanese, 新潟, Domestic conference

  Oral presentation


• 光圧によるαシヌクレイン液滴の時空間的な形成と老化の誘導

  茶谷絵理, 柚佳祐, Ching-Yang Lin, Po-Wei Yi, Chih-Hao Huang, 増原宏

  第15回 タンパク質の異常凝集とその防御・修復機構に関する研究会, Aug. 2024, Japanese, Web上での発表, Domestic conference

  [Invited]

  Invited oral presentation


• αB-crystallin prevents aging of α-synuclein droplets

  Kenji Fujitsuka, Keisuke Yuzu, Yuki Michiue, John A. Carver, Eri Chatani

  IUPAB2024, Jun. 2024, English, 京都, International conference

  Poster presentation


• Analysis of the aggregation characteristics of tau droplets under oxidizing and reducing conditions

  Yuki Michiue, Ayumi Masui, Keisuke Yuzu, Yumiko Ohhashi, Keiichi Yamaguchi, Yasushi Kawata, Eri Chatani

  IUPAB2024, Jun. 2024, English, 京都, International conference

  Poster presentation


• Spatiotemporal formation of a single liquid-like condensate of α-synuclein and subsequent aging by optical trapping

  Keisuke Yuzu, Ching-Yang Lin, Chih-Hao Huang, Po-Wei Yi, Hiroshi Masuhara, Eri Chatani

  IUPAB2024, Jun. 2024, English, 京都, International conference

  [Invited]

  Invited oral presentation


• Protein aggregation and diseases

  Motomasa Tanaka, Lukasz Joachimiak, Eri Chatani

  IUPAB2024, Jun. 2024, English, 京都, International conference

  [Invited]

  Public discourse


• Unveiling the mechanism of protein aggregation to form oligomers and protofibrils: A study using insulin as a model

  Eri Chatani, Keisuke Yuzu

  IUPAB2024, Jun. 2024, English, 京都, International conference

  [Invited]

  Invited oral presentation


• αB-クリスタリンによるαシヌクレイン液滴の老化阻害

  藤塚健次, 柚佳祐, 道上祐希, John A. Carver, 茶谷絵理

  第23回 日本蛋白質科学会年会, Jun. 2024, Japanese, 名古屋, Domestic conference

  Poster presentation


• タウ液滴の凝集に対する酸化還元状態の影響の解析

  道上佑希, 益井歩未, 柚佳祐, 大橋祐美子, 山口圭一, 河田康志, 茶谷絵理

  第23回 日本蛋白質科学会年会, Jun. 2024, Japanese, 名古屋, Domestic conference

  Poster presentation


• Spatiotemporal formation of a single liquid-like condensate of α-synuclein by optical trapping

  Keisuke Yuzu, Ching-Yang Lin, Po-Wei Yi, Chih-Hao Huang, Hiroshi Masuhara, Eri Chatani

  第24回 日本蛋白質科学会年会, Jun. 2024, English, 名古屋, Domestic conference

  [Invited]

  Invited oral presentation


• アミロイド線維の形成メカニズム－プロトフィブリ ルの形成プロセスに着目して―

  茶谷 絵理, 山本 直樹

  第24回 日本蛋白質科学会年会, Jun. 2024, Japanese, 名古屋, Domestic conference

  [Invited]

  Invited oral presentation


• マイクロ水滴を用いたタンパク質濃縮相からのアミロイド核生成速度解析法の開発

  川上純佳, 小澤 大樹, 丸山洋子, 大橋 祐美子, 茶谷 絵理, 白木 賢太郎, 火原 彰秀, 福山 真央

  日本分析化学会第84回分析化学討論会, May 2024, 京都, Domestic conference

  Poster presentation


• Exploring the mechanism and dynamics of protein aggregation into amyloid fibrils: From basics to recent observations on amyloid formation from liquid-like condensates

  Eri Chatani

  Department of Applied Chemistry, NYCU Colloquium, May 2024, English, 台湾新竹, International conference

  [Invited]

  Public discourse


• Optical trapping and single droplet formation of lysozyme at solution surface

  Yi-Sian Chien, Po-Wei Yi, Ching-Yang Lin, Shuichi Toyouchi, Keisuke Yuzu, Eri Chatani, Hiroshi Masuhara

  第71回 応用物理学会 春季学術講演会, Mar. 2024, English, 東京, Domestic conference, Co-authored internationally

  Oral presentation


• 酸化、還元条件下におけるタウ液滴の凝集機構の解明

  道上佑希, 大橋裕美子, 益井歩未, 柚佳祐, 山口圭一, 河田康志, 茶谷絵理

  神戸大学研究基盤センター 若手フロンティア研究会2023, Dec. 2023, Japanese, 神戸, Domestic conference

  Poster presentation


• 酸化条件下におけるタウ液滴の凝集機構の解明

  道上佑希, 大橋裕美子, 益井歩未, 柚佳祐, 山口圭一, 河田康志, 茶谷絵理

  第46回 日本分子生物学会年会, Dec. 2023, Japanese, 神戸, Domestic conference

  Poster presentation


• 液-液相分離に高い温度感受性をもたらすSup35天然変性領域の局所構造

  大橋 祐美子, 西奈美 卓, 白木 賢太郎, 茶谷 絵理

  第46回 日本分子生物学会年会, Dec. 2023, Japanese, 神戸, Domestic conference

  [Invited]

  Invited oral presentation


• アミロイド線維の生成場としての液滴の理解

  茶谷絵理, 大橋祐美子, 福山真央

  第46回 日本分子生物学会年会, Dec. 2023, Japanese, 神戸, Domestic conference

  [Invited]

  Invited oral presentation


• Optical-force induced spatiotemporal formation of a single liquid-like droplet of α-synuclein and subsequent maturation into amyloid fibrils

  Keisuke Yuzu, Ching-Yang Lin, Chih-Hao Huang, Po-Wei Yi, PinXun Huang, Hiroshi Masuhara, ○Eri Chatani

  16th International Symposium on Nanomedicine (ISNM2023), Nov. 2023, English, 大阪, International conference, Co-authored internationally

  [Invited]

  Invited oral presentation


• ウシ由来インスリンのアミロイドオリゴマーおよびプロトフィブリル形成のモデリング

  柚佳祐, 今村比呂志, 野崎拓郎, 藤井悠生, 守島健, 奥田綾, 井上倫太郎, 杉山正明, 茶谷絵理

  第61回 日本生物物理学会年会, Nov. 2023, English, 名古屋, Domestic conference

  Oral presentation


• Molecular mechanism of early inhibition of amyloid formation by αB-crystallin

  國尾佑貴, 柚佳祐, 山本直樹, 森島健, 奥田綾, 井上倫太郎, 杉山正明, Junna Hayashi, John A. Carve, 茶谷絵理

  第61回 日本生物物理学会年会, Nov. 2023, English, 名古屋, Domestic conference

  Poster presentation


• タンパク質濃縮相からのアミロイド核生成解析に向けた濃縮相サイズ調節法

  小澤大樹, 川上純佳, 佐藤安里, 大橋祐美子, 茶谷絵理, 丸山洋子, 白木賢太郎, 火原彰秀, 福山真央

  化学とマイクロ・ナノシステム学会 第48回研究会, Nov. 2023, Japanese, 熊本, Domestic conference

  Oral presentation


• 酵母プリオンタンパク質の液液相分離がアミロイド核生成に与える影響の評価

  福山 真央, 西奈美 卓, 冨田 峻介, 大橋 祐美子, 小澤大樹, 玄大雄, 粕谷 素洋, 茶谷 絵理, 丸山洋子, 白木 賢太郎, 火原 彰秀

  化学とマイクロ・ナノシステム学会 第48回研究会, Nov. 2023, Japanese, 熊本, Domestic conference


• アルファシヌクレイン凝集の長時間測定に向けた水中マイクロ流体デバイスの観察

  佐藤安里, 小澤大樹, 柚佳祐, 大橋祐美子, 茶谷絵理, 福山真央

  令和5年度 化学系学協会東北大会, Sep. 2023, Japanese, 仙台, Domestic conference

  Oral presentation


• タウ液滴の凝集に対する酸化還元状態の影響の解析

  道上佑希, 益井歩未, 大橋祐美子, 柚佳祐, 山口圭一, 後藤祐児, 河田康志, 茶谷絵理

  第23回 日本蛋白質科学会年会, Jun. 2023, Japanese, 名古屋, Domestic conference

  Poster presentation


• トランスサイレチン49-127断片のアミロイド線維形成反応における野生型と変異体の比較

  中尾 星哉, 柚 佳祐, 松村 美里, 大橋 祐美子, 茶谷 絵理

  第23回 日本蛋白質科学会年会, Jun. 2023, Japanese, 名古屋, Domestic conference

  Poster presentation


• ドデシル硫酸ナトリウム共存下でのαシヌクレイン凝集反応の多様性

  北野さくら, 柚佳祐, 笹田航, 木村哲就, 茶谷絵理

  第23回 日本蛋白質科学会年会, Jun. 2023, Japanese, 名古屋, Domestic conference

  Poster presentation


• ウシ由来インスリンのアミロイド核形成に見られるオリゴマーおよびプロトフィブリル形成機構

  柚佳祐, 今村比呂志, 野崎拓郎, 藤井悠生, 守島健, 奥田綾, 井上倫太郎, 杉山正明, 茶谷絵理

  第23回 日本蛋白質科学会年会, Jun. 2023, Japanese, 名古屋, Domestic conference

  Poster presentation


• Two-stage optical trapping mechanism of protein at its air/solution interface

  Po-Wei Yi, Wei-Hsiang Chiu, Shuichi Toyouchi, Roger Bresolí-Obach, Johan Hofkens, Eri Chatani, Yoichiroh Hosokawa, Teruki Sugiyama, Hiroshi Masuhara

  第70回応用物理学会春季学術講演会, Mar. 2023, English

  [Invited]

  Invited oral presentation


• "アミロイド前凝集体を標的とした 分子シャペロンαB-クリスタリンの線維形成阻害様式"

  茶谷絵理, 國尾祐貴, 柚佳祐, 山本直樹, 守島健, 奥田綾, 井上倫太郎, 杉山正明, Junna Hayashi, John, A. Carver

  第14回 タンパク質の異常凝集と その防御・修復機構に関する研究会, Mar. 2023, Japanese

  [Invited]

  Invited oral presentation


• アミロイド核形成段階におけるオリゴマーおよびプロトフィブリルの形成機構

  柚佳祐, 今村比呂志, 野崎拓郎, 藤井悠生, 守島健, 奥田綾, 井上倫太郎, 杉山正明, 茶谷絵理

  第14回 タンパク質の異常凝集と その防御・修復機構に関する研究会, Mar. 2023, Japanese

  [Invited]

  Invited oral presentation


• アミロイド線維の核形成メカニズムの解明

  茶谷絵理

  第14回ナノ・マイクロ計測化学セミナー, Mar. 2023, Japanese

  [Invited]

  Invited oral presentation


• Study of oligomer and protofibril mediated amyloid nucleation: Mechanistic insights from insulin and its B chain

  Eri Chatani

  JSPS Core to Core Program and Technical University of Munich Joint Meeting: Amyloid Symposium Munich 2023, Feb. 2023, English

  [Invited]

  Invited oral presentation


• トランスサイレチン49-127断片のアミロイド線維化反応の特徴と変異効果

  中尾星哉, 柚佳祐, 松村美里, 大橋祐美子, 茶谷絵理

  若手フロンティア研究会2022, Dec. 2022, Japanese

  Poster presentation


• ドデシル硫酸ナトリウムによるα-Synucleinの初期凝集体の捕捉と観察

  北野さくら, 柚佳祐, 笹田航, 木村哲就, 茶谷絵理

  若手フロンティア研究会2022, Dec. 2022, Japanese

  Poster presentation


• アミロイド線維の発生と伝播の分子機構

  茶谷絵理

  奈良先端科学技術大学院大学CDG共催光ナノセミナー, Nov. 2022, Japanese

  [Invited]

  Invited oral presentation


• Mechanisms of oligomer and protofibril formation suggested through studies of insulin and its B chain

  Eri Chatani

  "JSPS Core-to-Core Program and University of Wollongong Joint meeting: Protein aggregation, proteostasis and crocodiles", Nov. 2022, English

  [Invited]

  Invited oral presentation


• Local conformation of intrinsically disordered regions of Sup35 produces temperature sensitivity of liquid-liquid phase separation

  Yumiko Ohhashi, Suguru Nishinami, Kentaro Shiraki, Eri Chatani

  Proteostasis & Disease Symposium 2022, Nov. 2022, English

  Poster presentation


• Amyloid fibril formation of transthyretin induced by fragmentation

  Keisuke Yuzu, Misato Matsumura, Naoki Yamamoto, Masatomo So, Keiichi Yamaguchi, Yuji Goto, Eri Chatani

  Proteostasis & Disease Symposium 2022, Nov. 2022, English

  Poster presentation


• Formation of two types of tau oligomers depending on redox conditions

  Ayumi Masui, Keisuke Yuzu, Keiichi Yamaguchi, Yuji Goto, Yasushi Kawata, Eri Chatani

  Proteostasis & Disease Symposium 2022, Nov. 2022, English

  Poster presentation


• Conformational transitions of amyloid fibrils during cross-seeding

  Eri Chatani, Keisuke Yuzu, Naoki Yamamoto, Masahiro Noji, Masatomo So, Yuji Goto, Tetsushi Iwasaki, Motonari Tsubaki

  Proteostasis & Disease Symposium 2022, Nov. 2022, English

  Poster presentation


• The chaperone function of αB-crystallin through the formation of a complex with the amyloid precursor of the insulin B-chain

  Yuki Kokuo, Keisuke Yuzu, Naoki Yamamoto, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Junna Hayashi, John A. Carver, Eri Chatani

  Proteostasis & Disease Symposium 2022, Nov. 2022, English

  Poster presentation


• Liquid-liquid phase separation and amyloid formation of Sup35 from four different yeast species

  大橋 祐美子, 西奈美 卓, 白木 賢太郎, 茶谷 絵理

  第60回日本生物物理学会年会, Sep. 2022, English

  Poster presentation


• Amyloid fibril formation of transthyretin fragments

  柚佳祐, 松村美里, 山本直樹, 宗正智, 山口圭一, 後藤祐児, 茶谷絵理

  第60回日本生物物理学会年会, Sep. 2022, English

  Poster presentation


• Cooperative Optical Trapping Dynamics of Two Proteins at Solution Surface

  Wei-Hsiang Chiu, Po-Wei Yi, Shuichi Toyouchi, Yoichiroh Hosokawa, Roger Bresolí-Obach, Johan Hofkens, Eri Chatani, Hiroshi Masuhara

  第82回応用物理学会秋季学術講演会, Sep. 2022, English

  Oral presentation


• Study on solvent conditions of prion protein droplet formation for amyloid nucleation analysis

  Taiki Ozawa, Suguru Nishinami, Shunsuke Tomita, Yumiko Ohhashi, Motohiro Kasuya, Eri Chatani, Yoko Maruyama, Kentaro Shiraki, Akihide Hibara, Mao Fukuyama

  令和4年度化学系学協会東北大会, Sep. 2022, Japanese

  Poster presentation


• 近赤外分光法を用いた，タンパク質の２次構造変化に伴う水分子の水素結合ネットワークの変化についての分析

  石垣美歌, 加藤慎基, 茶谷絵理

  日本分析化学会第69年会, Sep. 2022, Japanese

  Oral presentation


• Fluorescence Imaging and Raman Spectral analyses of Optically Evolved Assembling of Lysozyme at Air/Solution Interface

  Po-Wei Yi, Shuichi Toyouchi, Wei-Hsiang Chiu, Roger Bresolí-Obach, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, Hiroshi Masuhara

  2022年光化学討論会, Sep. 2022, English

  [Invited]

  Invited oral presentation


• アミロイド線維の形成－核生成と伝播－の機構解明

  茶谷 絵理

  広島大学 第25回RcMcD融合研究セミナー, Jul. 2022, Japanese

  [Invited]

  Invited oral presentation


• トランスサイレチン49-127断片のアミロイド線維化反応における塩の影響

  中尾 星哉, 柚 佳祐, 松村 美里, 大橋 祐美子, 茶谷 絵理

  第22回日本蛋白質科学会年会, Jun. 2022, Japanese

  Oral presentation


• Elucidation of the early aggregation mechanism of alpha-synuclein amyloid fibril formation

  北野さくら, 柚佳祐, 笹田航, 木村哲就, 茶谷絵理

  第22回日本蛋白質科学会年会, Jun. 2022, Japanese

  Oral presentation


• タウのアミロイド線維形成に対する酸化還元状態の影響

  益井歩未, 柚佳祐, 山口圭一, 後藤祐児, 河田康志, 茶谷絵理

  第22回日本蛋白質科学会年会, Jun. 2022, Japanese

  Oral presentation


• Small heat shock protein αB-crystallin forms the complex with amyloid precursor of insulin B chain and inhibits its growth

  國尾祐貴, 柚佳祐, 山本直樹, Junna Hayashi, John, A. Carver, 守島健, 井上倫太郎, 杉山正明, 茶谷絵理

  第22回日本蛋白質科学会年会, Jun. 2022, Japanese

  Oral presentation


• Amyloid fibril formation of fragmented transthyretin

  柚佳祐, 松村美里, 山本直樹, 宗正智, 山口圭一, 茶谷絵理

  第22回日本蛋白質科学会年会, Jun. 2022, Japanese

  Oral presentation


• Sup35天然変性領域の局所構造が液-液相分離の環境応答性を決定する

  大橋祐美子, 西奈美卓, 白木賢太郎, 茶谷絵理

  第22回日本蛋白質科学会年会, Jun. 2022, Japanese

  Oral presentation


• 液液相分離相からのアミロイド形成速度のサイズ依存

  福山 真央, 西奈美 卓, 冨田 峻介, 大橋 祐美子, 粕谷 素洋, 茶谷 絵理, 丸山洋子, 白木 賢太郎, 火原 彰秀

  化学とマイクロ・ナノシステム学会 第45回研究会, May 2022, Japanese

  Oral presentation


• マイクロサイズプリオンタンパク質集合液滴中からのアミロイド核生成速度解析

  福山 真央, 西奈美 卓, 冨田 峻介, 大橋 祐美子, 粕谷 素洋, 茶谷 絵理, 白木 賢太郎, 火原 彰秀

  第82回分析化学討論会, May 2022, Japanese

  Oral presentation


• Investigating protein assembly in the nucleation of amyloid fibrils

  Eri Chatani

  Molecular Basis of Proteinopathies, May 2022, English

  [Invited]

  Invited oral presentation


• アミロイド線維形成の初期に伴うタンパク質の集合・動態

  茶谷絵理

  松ヶ崎サイエンスフォーラム, Apr. 2022, Japanese, Web上での発表, Japan, Domestic conference

  [Invited]

  Public discourse


• Protein Assembly Formation by Cooperative Optical Trapping with Polyethylene Glycol at Solution Surface

  Wei-Hsiang Chiu, Po-Wei. Yi, Eri Chatani, Roger Bresolí-Obach, Johan Hofkens, Shuichi Toyouchi, Hiroshi Masuhara

  第69回応用物理学会春季学術講演会, Mar. 2022, English, 青山学院大相模原キャンパスとオンライン, Japan, Domestic conference

  Oral presentation


• Determination of amyloid nucleation rate in Sup35 droplet

  Mao Fukuyama, Suguru Nishinami, Shunsuke Tomita, Motohiro Kasuya, Yumiko Ohhashi, Eri Chatani, Kentaro Shiraki, Akihide Hibara

  第二回分子夾雑化学国際会議, Dec. 2021, English, 東京ビッグサイト, Japan, International conference

  Poster presentation


• タウのオリゴマー形成に対する酸化還元状態の影響

  益井歩未, 柚佳祐, 山口圭一, 後藤祐児, 河田康志, 茶谷絵理

  若手フロンティア研究会 2021, Dec. 2021, Japanese, 神戸大学, Japan, Domestic conference

  Poster presentation


• αB-クリスタリンのアミロイド線維形成阻害機構

  國尾 祐貴, 柚 佳祐, Junna Hayashi, John A. Caver, 茶谷 絵理

  若手フロンティア研究会 2021, Dec. 2021, Japanese, 神戸大学, Japan, Domestic conference

  Poster presentation


• Early aggregation mechanisms preceding the amyloid nucleation of bovine and human insulin

  Keisuke Yuzu, Hiroshi Imamura, Takuro Nozaki, Yuki Fujii, Ken Morishima, Aya Okuda, Rintaro Inoue, Masaaki Sugiyama, Eri Chatani

  若手フロンティア研究会 2021, Dec. 2021, Japanese, 神戸大学, Japan, Domestic conference

  Poster presentation


• Mechanism of protein assembly for the formation of amyloid fibrils

  Eri Chatani

  CEFMS Lecture on Protein Dynamics, Dec. 2021, English, Web上での発表, Taiwan, Province of China, International conference

  [Invited]

  Invited oral presentation


• Understanding protein aggregation towards amyloid fibrils

  茶谷 絵理

  第44回日本分子生物学会年会, Dec. 2021, Japanese, パシフィコ横浜, Japan

  [Invited]

  Poster presentation


• Inhibition of amyloid fibrillation by αB-crystallin by preventing the growth of prefibrillar intermediates: Formation of a stable complex

  Yuki Kokuo, Keisuke Yuzu, Junna Hayashi, John A. Carver, Eri Chatani

  第59回日本生物物理学会年会, Nov. 2021, English, Web上での発表, Japan, Domestic conference

  Oral presentation


• Early aggregation kinetics leading to the amyloid nucleation of bovine and human insulin

  Keisuke Yuzu, Hiroshi Imamura, Takuro Nozaki, Yuki Fujii, Ken Morishima, Aya Okuda, Rintaro Inoue, Masaaki Sugiyama, Keisuke Tominaga, Eri Chatani

  第59回日本生物物理学会年会, Nov. 2021, English, Web上での発表, Japan, Domestic conference

  Oral presentation


• Analysis of Heparin-Induced Tau Oligomer Formation by Dynamic Light Scattering

  Ayumi Masui, Keisuke Yuzu, Keiichi Yamaguchi, Yuji Goto, Yasushi Kawata, Eri Chatani

  第59回日本生物物理学会年会, Nov. 2021, English, Web上での発表, Japan, Domestic conference

  Oral presentation


• Mechanism of inhibition of amyloid fibril formation by alphaB-crystallin action on prefibrillar intermediates

  國尾祐貴, 柚佳祐, John A. Carver, 茶谷絵理

  第94回日本生化学大会, Nov. 2021, Japanese, Web上での発表, Japan, Domestic conference

  Oral presentation


• Early aggregation mechanisms preceding the amyloid nucleation of bovine and human insulin

  Keisuke Yuzu, Hiroshi Imamura, Takuro Nozaki, Yuki Fujii, Ken Morishima, Aya Okuda, Rintaro Inoue, Masaaki Sugiyama, Keisuke Tominaga, Eri Chatani

  第94回日本生化学大会, Nov. 2021, Japanese, Web上での発表, Japan, Domestic conference

  Oral presentation


• Analysis of Oligomer Formation of Tau by Dynamic Light Scattering

  益井歩未, 柚佳祐, 後藤祐児, 山口圭一, 河田康志, 茶谷絵理

  第94回日本生化学大会, Nov. 2021, Japanese, Web上での発表, Japan, Domestic conference

  Poster presentation


• Optically evolving condensation of protein: A sub-millimeter 3-dimensional domain formation from solution surface

  Shuichi TOYOUCHI, Po-Wei YI, Wei-Hsiang CHIU, Roger Bresolí-OBACH, Johan HOFKENS, Eri CHATANI, Teruki SUGIYAMA, Ryohei YASUKUNI, Yoichiroh HOSOKAWA, Hiroshi MASUHARA

  2021年光化学討論会, Sep. 2021, English, Web上での発表, Japan, Domestic conference

  Oral presentation


• Optically Evolved Condensation of Lysozyme Study by Raman Microspectroscopy

  Po-Wei Yi, Wei-Hsiang Chiu, Tetsuhiro Kudo, Roger Bresoli-Obach, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, Shuichi Toyouchi, Hiroshi Masuhara

  第82回応用学会秋季学術講演会, Sep. 2021, English, Web上での発表, Japan, Domestic conference

  Oral presentation


• Optical force-induced three-dimensional protein assembly growing from solution

  Roger Bresolí-Obach, Po-Wei Y, Wei-Hsiang Chiu, Tetsuhiro Kudo, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, Shuichi Toyouchi, Hiroshi Masuhara

  19th Congress of the European Society for Photobiology, Aug. 2021, English, Salzburg, Austria, International conference

  Poster presentation


• Optical force-induced three-dimensional protein assembly growing from solution surface

  Po-Wei Yi, Wei-Hisang Chiu, Tetsuhiro Kudo, Roger Bresolí-Obach, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, Shuichi Toyouchi, Hiroshi Masuhara

  International Conference on Photochemistry 2021, Jul. 2021, English, Web上での発表, International conference

  Oral presentation


• Sup35 の液- 液相分離におけるプリオンドメインの役割

  大橋 祐美子, 西奈美 卓, 白木 賢太郎, 茶谷 絵理

  第21回日本蛋白質科学会年会, Jun. 2021, Japanese, Web上での発表, Japan, Domestic conference

  Poster presentation


• インスリンアミロイド線維の種間クロスシーディングに見られる構造の変化

  柚 佳祐, 山本 直樹, 野地 真広, 宗 正智, 後藤 祐児, 岩崎 哲史, 鍔木 基成, 茶谷 絵理

  第21回日本蛋白質科学会年会, Jun. 2021, Japanese, Web上での発表, Japan, Domestic conference

  Poster presentation


• Early aggregation kinetics preceding the nucleation in distinct amyloid formation pathways between bovine and human insulin

  柚 佳祐, 今村 比呂志, 野崎 拓郎, 守島 健, 奥田 綾, 井上 倫太郎, 杉山 正明, 茶谷 絵理

  第21回日本蛋白質科学会年会, Jun. 2021, Japanese

  Poster presentation


• Thioflavin T fluorescence lifetime assay for distinguishing diverse structures of insulin amyloid fibrils

  小田 明典, 犬伏 知生, 柚 佳祐, 茶谷 絵理, 吉川 悦次, 里園 浩

  第21回日本蛋白質科学会年会, Jun. 2021, Japanese

  Poster presentation


• Single Sub-Millimeter Linear Assembly of Polystyrene Microparticles

  Po-Wei. Yi, Wei-Hsiang Chiu, Tetsuhiro Kudo, Roger Bresolí-Obach, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, Shuichi Toyouchi, Hiroshi Masuhara

  第68回応用物理学会春季学術講演会, Mar. 2021, English

  Oral presentation


• Optical Trapping and Assembling of Protein at Solution Surface

  Po-Wei. Yi, Tetsuhiro Kudo, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, Hiroshi Masuhara

  The 81th JSAP Autumn Meeting, Sep. 2020, English

  Oral presentation


• Investigation between amyloid fibril formation and phospholipid bilayer destruction

  高山 宙輝, 曲師 香緒里, 茶谷 絵理

  第93回日本生化学会大会, Sep. 2020, Japanese

  Oral presentation


• Multi-step conformational changes via intermediate states induced by cross-seeding of human and bovine insulin amyloid fibrils

  Keisuke Yuzu, Naoki Yamamoto, Masahiro Noji, Masatomo So, Yuji Goto, Tetsushi Iwasaki, Motonari Tsubaki, Eri Chatani

  第93回日本生化学会大会, Sep. 2020, English

  Oral presentation


• Investigation of relationships between amyloid fibril formation and phospholipid bilayer destruction

  Hiroki Takayama, Kaori Mageshi, Kenichi Morigaki, Eri Chatani

  第58回日本生物物理学会年会, Sep. 2020, English

  Poster presentation


• Cross-seeding of human and bovine insulin amyloid fibrils induces stepwise conformational transition via intermediate states

  Keisuke Yuzu, Naoki Yamamoto, Masahiro Noji, Masatomo So, Yuji Goto, Tetsushi Iwasaki, Motonari Tsubaki, Eri Chatani

  第58回日本生物物理学会年会, Sep. 2020, English

  Poster presentation


• Thioflavin T Fluorescence Lifetime Assay Of Homogeneous Insulin Amyloid Fibrils

  Akinori Oda, Tomoo Inubushi, Keisuke Yuzu, Eri Chatani, Etsuji Yoshikawa, Hiroshi Satozono

  第20回日本蛋白質科学会年会, Jul. 2020, English

  Others


• Amyloid Formation And Breakdown Of Supersaturation

  Masahiro Noji, Keiichi Yamaguchi, Eri Chatani, Yuji Goto

  第20回日本蛋白質科学会年会, Jul. 2020, English

  Others


• Structural difference and its seed-dependent propagation manner of human/bovine insulin amyloid fibrils as detected by iodine staining

  Keisuke Yuzu, Naoki Yamamoto, Masahiro Noji, Masatomo So, Yuji Goto, Tetsushi Iwasaki, Eri Chatani

  第20回日本蛋白質科学会, Jul. 2020, English

  Others


• Use of iodine staining as a sensitive probe to distinguish diverse structures of insulin amyloid fibrils

  Eri Chatani, Takato Hiramatsu, Ha Seongmin, Yuki Masuda, Mitsuru Yasuda, Mika Ishigaki, Keisuke Yuzu, Yukihiro Ozaki

  4. Ulm Meeting – Biophysics of Amyloid Formation, Feb. 2020, English

  Poster presentation


• アミロイド核形成メカニズムの解明を目指した核形成中間体の捕捉と構造解析

  吉川 祐樹, 山本 直樹, 井上 倫太郎, 守島 健, 杉山 正明, 田村 厚夫, 茶谷 絵理

  京都大学原子炉実験所 第54回学術講演会, Feb. 2020, Japanese

  Poster presentation


• X線小角散乱によるアミロイド線維前駆中間体発達の時分割追跡

  山本 直樹, 赤井 大気, 井上 倫太郎, 杉山 正明, 柴山 直哉, 茶谷 絵理

  京都大学原子炉実験所 第54回学術講演会, Feb. 2020, Japanese

  Poster presentation


• Observation of phospholipid membrane disruption associated with the fibrillation of amyloid β peptide

  Eri Chatani

  One-Day Discussion Meeting on Dynamics of Chemical and Biological Systems, Jan. 2020, English

  [Invited]

  Invited oral presentation


• Round Table: Diagnosis and Therapies

  Masaru Hoshino, Eri Chatani

  Towards a cure for amyloid diseases: A successful example of precision and translational medicine, Dec. 2019, English

  [Invited]

  Nominated symposium


• ヨウ素染色を用いたアミロイド構造の識別及びシード依存的構造伝播の追跡

  柚 佳祐, 茶谷 絵理

  神戸大学 研究基盤センター 若手フロンティア研究会2019, Dec. 2019, Japanese

  Poster presentation


• 液-液相分離の不可逆性によるアミロイド線維形成反応の促進および抑制

  桐山 知樹, 今村 比呂志, 茶谷 絵理

  神戸大学 研究基盤センター 若手フロンティア研究会2019, Dec. 2019, Japanese

  Poster presentation


• イン スリアミロイド の伸長反応によるリポソームの膜破壊

  高山 宙輝, 曲師 香緒里, 茶谷 絵理

  神戸大学 研究基盤センター 若手フロンティア研究会2019, Dec. 2019, Japanese

  Poster presentation


• プロテアーゼがトランスサイレチンのアミロイド線維形成に与える効果の検証

  松村 美里, 山本 直樹, 山口 圭一, 宗 正智, 後藤 祐児, 茶谷 絵理

  神戸大学 研究基盤センター 若手フロンティア研究会2019, Dec. 2019, Japanese

  Poster presentation


• Observation of prefibrillar intermediates of insulin B chain and their stabilization by interaction with fibrinogen

  Eri Chatani, Naoki Yamamoto, Taiki Akai, Shoko Tsuhara, Atsuo Tamura, Rintaro Inoue, Masaaki Sugiyama

  Towards a cure for amyloid diseases: A successful example of precision and translational medicine, Dec. 2019, English

  Poster presentation


• ヨウ素染色で見るアミロイド線維構造の多様性と伝播様式

  茶谷 絵理, 平松 貴人, 柚 佳祐, 山本 直樹

  第42回日本分子生物学会年会, Dec. 2019, Japanese

  [Invited]

  Invited oral presentation


• Iodine staining for identifying amyloid polymorphism and for tracking seed-dependent structural propagation

  Eri Chatani, Takato Hiramatsu, Keisuke Yuzu, Naoki Yamamoto, Mitsuru Yasuda, Mika Ishigaki, Yukihiro Ozaki

  Indo-Japan Workshop-Frontiers in Molecular Spectroscopy: From Fundamentals to Applications in Chemistry and Biology, Nov. 2019, English

  [Invited]

  Invited oral presentation


• ヨウ素染色によるアミロイド線維の構造伝播の追跡

  柚 佳祐, 茶谷 絵理

  神戸大学 第10回サイエンスフロンティア研究発表会, Oct. 2019, Japanese

  Poster presentation


• アミロイド核形成メカニズムの解明を目指した核形成中間体の捕捉と構造解析

  吉川 祐樹, 山本 直樹, 井上 倫太郎, 杉山 正明, 田村 厚夫, 茶谷 絵理

  神戸大学 第10回サイエンスフロンティア研究発表会, Oct. 2019, English

  Poster presentation


• Iodine staining as a useful probe for amyloid polymorphism and its propagation

  Eri Chatani, Takato Hiramatsu, Keisuke Yuzu, Naoki Yamamoto

  第57回日本生物物理学会年会, Sep. 2019, English

  [Invited]

  Invited oral presentation


• Examination of the possibility of the formation of transmissible transthyretin amyloid fibrils by the use of proteolysis

  松村 美里, 山本 直樹, 山口 圭一, 宗 正智, 後藤 祐児, 茶谷 絵理

  第57回日本生物物理学会年会, Sep. 2019, English

  Poster presentation


• Structural difference and its seed-dependent propagation of bovine/human insulin amyloid fibrils as detected by iodine staining

  Keisuke Yuzu, Eri Chatani

  第57回日本生物物理学会年会, Sep. 2019, English

  Poster presentation


• Understanding molecular mechanisms of amyloid fibril nucleation: An observation of a step-by-step aggregation pathway of insulin B chain

  Eri Chatani

  Seminar of Institute of Chemistry, UPLB, Sep. 2019, English

  [Invited]

  Invited oral presentation


• Effect of protease on amyloid fibril formation of transthyretin

  松村 美里, 山本 直樹, 山口 圭一, 宗 正智, 後藤 祐児, CHATANI ERI

  第19回日本蛋白質科学会年会･第71回日本細胞生物学会大会 合同年次大会, Jun. 2019, English, 神戸国際会議場, Domestic conference

  Poster presentation


• Study of structural features and recognition mechanism of NLRP3-LRR domain involved in recognition of amyloid-β fibril

  政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, MATSUHANA SAORI, INOUE KUNIO, TSUBAKI MOTONARI, CHATANI ERI

  第19回日本蛋白質科学会年会･第71回日本細胞生物学会大会 合同年次大会, Jun. 2019, English, 神戸国際会議場, Domestic conference

  Poster presentation


• Trapping and structural analysis of nucleation intermediates aimed at clarifying amyloid nucleation mechanisms

  Yuhki Yoshikawa, Atsuo Tamura, Naoki Yamamoto, CHATANI ERI

  第19回日本蛋白質科学会年会･第71回日本細胞生物学会大会 合同年次大会, Jun. 2019, English, 神戸国際会議場, Domestic conference

  Poster presentation


• Exploring the relationship between liquid-liquid phase separation and amyloid formation of lysozyme solution

  桐山 知樹, CHATANI ERI

  第19回日本蛋白質科学会年会･第71回日本細胞生物学会大会 合同年次大会, Jun. 2019, English, 神戸国際会議場, Domestic conference

  Poster presentation


• Elucidation of molecular functions of human tumor suppressor protein 101F6 by reconstitution into phospholipid bilayer nanodiscs

  Mohamed El-Behery, Akikazu Asada, Fusako Takeuchi, Tetsunari Kimura, Eri Chatani, ○Motonari Tsubaki

  34TH PHILIPPINE CHEMISTRY CONGRESS, May 2019, English, Waterfront Hotel and Convention Center（Philippines）, International conference

  Oral presentation


• アミロイドβの凝集に伴うリン脂質膜破壊の観察

  CHATANI ERI

  第4回近畿大学認知症Core研究(DoIK)シンポジウム「認知症克服に向けた工学的アプローチ; How Engineering Approaches Fight against Dementia」, Mar. 2019, Japanese, 近畿大学東大阪キャンパス, Domestic conference

  Oral presentation


• フィブリノーゲンによるインスリンB鎖アミロイド線維の形成阻害

  CHATANI ERI, 山本 直樹, 赤井 大気, 井上 倫太郎, 杉山 正明, TAMURA ATSUO

  京都大学複合原子力科学研究所 第53回学術講演会プログラム, Feb. 2019, Japanese, 京都大学複合原子力科学研究所, Domestic conference

  Poster presentation


• Exploring multi-step nucleation mechanisms of amyloid fibrils

  Eri Chatani

  Ulm Meeting on “Biophysics of Amyloid Formation”, Feb. 2019, English, Ulm University, Germany, International conference

  [Invited]

  Invited oral presentation


• Enhanced membrane disruption by Aβ1-40 under weak acidic conditions

  ○Kaori Mageshi, Naoki Yamamoto, Ryota Komatsu, Kenichi Morigaki, Masatomo So, Yuji Goto, Eri Chatani

  Ulm Meeting on “Biophysics of Amyloid Formation”, Feb. 2019, English, Ulm University, Germany, International conference

  Poster presentation


• リゾチームにおける液-液相分離とアミロイド線維形成との関係性

  桐山 知樹, 茶谷 絵理

  非共有結合系の分子科学：計測技術から探る生体分子科学の新展開/研究プロジェクト「非共有結合系分子科学研究」, Jan. 2019, English, 神戸大学 理学部Z201・Z202号室, Domestic conference

  Poster presentation


• トランスサイレチンのアミロイド線維化に対するプロテアーゼの効果

  松村 美里, 山本 直樹, 茶谷 絵理

  非共有結合系の分子科学：計測技術から探る生体分子科学の新展開/研究プロジェクト「非共有結合系分子科学研究」, Jan. 2019, English, 神戸大学 理学部Z201・Z202号室, Domestic conference

  Poster presentation


• アミロイドβ線維認識に関与するNLRP3-LRRドメインの構造特徴および認識機構の解明

  政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, MATSUHANA SAORI, INOUE KUNIO, TSUBAKI MOTONARI, CHATANI ERI

  非共有結合系の分子科学：計測技術から探る生体分子科学の新展開/研究プロジェクト「非共有結合系分子科学研究」, Jan. 2019, Japanese, 神戸大学理学研究科Z201,202, Domestic conference

  Poster presentation


• Structural Characterization of Amyloid Nucleation Intermediates of Insulin B Chain

  ○Yuhki Yoshikawa, Naoki Yamamoto, Atsuo Tamura, Eri Chatani

  非共有結合系の分子科学：計測技術から探る生体分子科学の新展開/研究プロジェクト「非共有結合系分子科学研究」, Jan. 2019, English, 神戸大学 理学部Z201・Z202号室, Domestic conference

  Poster presentation


• 炎症誘起タンパク質NLRP3のLRRドメインによるアミロイドβ線維の直接的認識

  政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, MATSUHANA SAORI, INOUE KUNIO, TSUBAKI MOTONARI, CHATANI ERI

  神戸大学 研究基盤センター 若手フロンティア研究会, Dec. 2018, Japanese, 神戸大学百年記念館, Domestic conference

  Poster presentation


• ヨウ素染色によるアミロイド構造多形の識別の試み

  CHATANI ERI

  第10回 タンパク質の異常凝集とその防御・修復機構に関する研究会, Dec. 2018, Japanese, 京都大学複合原子力科学研究所, Domestic conference

  [Invited]

  Invited oral presentation


• アミロイド核形成メカニズムの解明を目指した核形成中間体の捕捉および構造解析

  吉川 祐樹, 山本 直樹, TAMURA ATSUO, CHATANI ERI

  神戸大学 研究基盤センター 若手フロンティア研究会, Dec. 2018, Japanese, 神戸大学百年記念館, Domestic conference

  Poster presentation


• Investigation of aggregation behavior of insulin B chain in the nucleation of amyloid fibrils

  Eri Chatani

  Indo-Japan Joint Meeting ʺLocal Structure and Dynamics of Hydrogen Bonds in Water: Super Cooled Water and Binary Mixtureʺ, Dec. 2018, English, Indian Institute of Science, Bangalore, INDIA, International conference

  [Invited]

  Invited oral presentation


• Biophysical investigation of protein aggregation that causes the onset of amyloid diseases

  Eri Chatani

  なでしこScientistトーク, Dec. 2018, English, 甲南大学 岡本キャンパス, International conference

  [Invited]

  Invited oral presentation


• アミロイド核形成の機構解明を目指して―多段階的な反応プロセスの観察

  CHATANI ERI

  東京工業大学セミナー, Oct. 2018, Japanese, 東京工業大学すずかけ台キャンパス, Domestic conference

  Oral presentation


• アミロイド線維の核形成に見られるタンパク質分子の集合挙動

  CHATANI ERI

  新学術領域「中分子戦略」「分子夾雑化学」ジョイントシンポジウム 第21回生命化学研究会, Sep. 2018, Japanese, 大阪大学 豊中キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• Structural comparison of amyloid nucleation intermediates of insulin B chain formed at different pHs

  ○Yuhki Yoshikawa, Naoki Yamamoto, Atsuo Tamura, Eri Chatani

  第56回日本生物物理学会年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Poster presentation


• NLRP3におけるLRRドメインの構造特徴およびアミロイドβ線維認識機構の解明

  政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, TSUBAKI MOTONARI, CHATANI ERI

  第91回日本生化学会大会, Sep. 2018, Japanese, 国立京都国際会館, Domestic conference

  Poster presentation


• Investigation of multi-step nucleation of insulin B chain amyloid fibrils and its inhibition

  Naoki Yamamoto, Taiki Akai, Shoko Tsuhara, Rintaro Inoue, Masaaki Sugiyama, Atsuo Tamura, ○Eri Chatani

  第56回日本生物物理学会年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Oral presentation


• タンパク質の構造物性から考えるアミロイドーシス発症のメカニズム

  CHATANI ERI

  基盤研セミナー, Aug. 2018, Japanese, 医薬基盤・健康・栄養研究所, Domestic conference

  [Invited]

  Invited oral presentation


• タンパク質・酵素の構造形成と凝集について

  CHATANI ERI

  日本生物高分子学会テクニカルワークショップ 2018年度 酵素取扱者講習会, Jul. 2018, Japanese, 龍谷大学大阪梅田キャンパス, Domestic conference

  Public discourse


• 様々なpHにおけるインスリンB鎖のアミロイド核形成反応中間体の観察

  吉川 祐樹, 山本 直樹, TAMURA ATSUO, CHATANI ERI

  第18回日本蛋白質科学会 ワークショップ「分子夾雑の蛋白質科学」, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• 様々なpHにおけるインスリンB鎖のアミロイド核形成反応中間体の観察

  吉川 祐樹, 山本 直樹, TAMURA ATSUO, CHATANI ERI

  第18回日本蛋白質科学会, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• 分子夾雑環境での蛋白質凝集反応-アミロイド線維形成にもたらす他分子の共存効果-

  CHATANI ERI, 後藤 祐児

  第18回日本蛋白質科学会 ワークショップ「分子夾雑の蛋白質科学」, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  [Invited]

  Invited oral presentation


• 弱酸性条件におけるAβ1-40のアミロイド線維形成と脂質膜破壊

  曲師 香緒里, 山本 直樹, MORIGAKI KENICHI, CHATANI ERI

  第18回日本蛋白質科学会, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• 細胞のアミロイドβ線維認識に関わるNLRP3-LRRドメインの構造特徴の解明

  政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, TSUBAKI MOTONARI, CHATANI ERI

  第18回日本蛋白質科学会 ワークショップ「分子夾雑の蛋白質科学」, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• 細胞のアミロイドβ線維認識に関わるNLRP3-LRRドメインの構造特徴の解明

  政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, TSUBAKI MOTONARI, CHATANI ERI

  第18回日本蛋白質科学会, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• タンパク質の広帯域誘電分光；水和水ダイナミクスおよびその熱活性の検証

  山本 直樹, 伊藤 奨太, OHTA KAORU, 中西 真大, TAMURA ATSUO, 井上 圭一, 神取 秀樹, CHATANI ERI, TOMINAGA KEISUKE

  第18回日本蛋白質科学会 ワークショップ「分子夾雑の蛋白質科学」, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• タンパク質の広帯域誘電分光；水和水ダイナミクスおよびその熱活性の検証

  山本 直樹, 伊藤 奨太, OHTA KAORU, 中西 真大, TAMURA ATSUO, 井上 圭一, 神取 秀樹, CHATANI ERI, TOMINAGA KEISUKE

  第18回日本蛋白質科学会, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• Exploring the mechanisms of protein self-assembly underlying amyloid nuclei formation

  ○Eri Chatani, Naoki Yamamoto

  新学術領域「動的秩序と機能」第6回国際シンポジウム, Jan. 2018, English, TKP浜松アクトタワーカンファレンスセンター, Domestic conference

  Poster presentation


• Characterization and stabilization of prefibrillar aggregate of insulin B chain for inhibition of amyloid fibril formation

  ○Naoki Yamamoto, Taiki Akai, Shoko Tsuhara, Atsuo Tamura, Rintaro Inoue, Masaaki Sugiyama, Eri Chatani

  新学術領域「動的秩序と機能」第6回国際シンポジウム, Jan. 2018, English, TKP浜松アクトタワーカンファレンスセンター, Domestic conference

  Poster presentation


• BROADBAND DIELECTRIC SPECTROSCOPY ON PROTEINS AND LIPID BILAYERS FROM SUB-GHZ TO THZ

  Naoki Yamamoto, Yu Kadomura, Shota Ito, Masahiro Nakanishi, Eri Chatani, Kei-ichi Inoue, Hideki Kandori, ○Keisuke Tominaga

  13th DAE-BRNS Biennial Trombay Symposium onRadiation & Photochemistry, Jan. 2018, English, インド, International conference

  [Invited]

  Invited oral presentation


• 線維前駆中間体の観察によるアミロイド核形成の機構解明と制御

  CHATANI ERI

  蛋白質異常凝集研究会, Dec. 2017, Japanese, 龍谷大学大阪キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• トランスサイレチン凝集反応のpH および塩濃度依存性の解析

  下地 恭平, 山本 直樹, 茶谷 絵理

  若手フロンティア研究会2017, Dec. 2017, Japanese, 神戸大学百年記念館, Domestic conference

  Poster presentation


• Aβ1-40のアミロイド線維形成と膜破壊能に 対するpHの効果

  曲師 香緒里, 山本 直樹, 平松 貴人, 茶谷 絵理

  若手フロンティア研究会2017, Dec. 2017, Japanese, 神戸大学百年記念館, Domestic conference

  Poster presentation


• 弱酸性条件におけるAβ1-40のアミロイド線維形成と膜破壊機構の解明

  曲師 香緒里, YAMAMOTO NAOKI, 平松 貴人, CHATANI ERI

  第4回 新学術領域研究「動的秩序と機能」若手研究会, Nov. 2017, Japanese, アイアイランド（大阪府四條畷市）, Domestic conference

  Poster presentation


• pHおよび塩濃度に依存したトランスサイレチン凝集反応の観察

  下地 恭平, YAMAMOTO NAOKI, CHATANI ERI

  第4回 新学術領域研究「動的秩序と機能」若手研究会, Nov. 2017, Japanese, アイアイランド（大阪府四條畷市）, Domestic conference

  Poster presentation


• Amyloid fibrillation of Aβ1-40 under weak acidic conditions

  Kaori Mageshi, YAMAMOTO NAOKI, Takato Hiramatsu, CHATANI ERI

  第55回日本生物物理学会年会, Sep. 2017, Japanese, 熊本大学 黒髪北地区, Domestic conference

  Poster presentation


• Investigation of inhibition mechanism of fibrinogen in the amyloid fibrillation

  Taiki Akai, YAMAMOTO NAOKI, CHATANI ERI

  第55回日本生物物理学会年会, Sep. 2017, Japanese, 熊本大学 黒髪北地区, Domestic conference

  Oral presentation


• A specific form of prefibrillar aggregates that functions as a precursor of amyloid nucleation

  YAMAMOTO NAOKI, Shoko Tsuhara, TAMURA ATSUO, CHATANI ERI

  第55回日本生物物理学会年会, Sep. 2017, Japanese, 熊本大学 黒髪北地区, Domestic conference

  Oral presentation


• Characterization of prefibillar intermediates that functionas a precursorof the amyloid fibrils

  ○Naoki Yamamoto, Shoko Tsuhara, Atsuo Tamura, Eri Chatani

  Horizon in Molecular Biology 2017, Sep. 2017, English, ドイツ ゲッティンゲン, International conference

  Poster presentation


• 超音波照射によるインスリンB 鎖アミロイド前駆中間体の線維化誘導

  YAMAMOTO NAOKI, 津原 祥子, TAMURA ATSUO, CHATANI ERI

  第17回日本蛋白質科学会年会, Jun. 2017, Japanese, 仙台国際センター, Domestic conference

  Poster presentation


• 弱酸性条件におけるAβ1-40アミロイド線維形成反応の観察

  曲師 香緒里, 平松 貴人, YAMAMOTO NAOKI, CHATANI ERI

  第17回日本蛋白質科学会年会, Jun. 2017, Japanese, 仙台国際センター, Domestic conference

  Poster presentation


• アミロイド形成における蛋白質分子集合プロセスの観察

  CHATANI ERI

  第17回日本蛋白質科学会年会, Jun. 2017, Japanese, 仙台国際センター, Domestic conference

  [Invited]

  Invited oral presentation


• Role of prefibrillar intermediates of insulin B chain in its amyloid fibril formation

  ○Naoki Yamamoto, Shoko Tsuhara, Atsuo Tamura, Eri Chatani

  新学術領域「動的秩序と機能」第5回国際シンポジウム, Jan. 2017, English, 東京大学駒場キャンパス, International conference

  Poster presentation


• Nucleation dynamics in amyloid formation as investigated by the analysis of prefibrillar intermediates

  ○Eri Chatani, Naoki Yamamoto

  新学術領域「動的秩序と機能」第5回国際シンポジウム, Jan. 2017, English, 東京大学駒場キャンパス, International conference

  Oral presentation


• A New fibrinogen function of trapping amyloid intermediate conformation

  ○Taiki Akai, Naoki Yamamoto, Eri Chatani

  新学術領域「動的秩序と機能」第5回国際シンポジウム, Jan. 2017, English, 東京大学駒場キャンパス, International conference

  Poster presentation


• Structural analysis of insulin amyloid fibril in the formation process by Raman spectroscopy

  〇Kana Morimoto, Mika Ishigaki, CHATANI ERI, Roumiana Tsenkova, Yukihiro Ozaki

  Japan-Taiwan Medical Spectroscopy International Symposium, Dec. 2016, English, 淡路夢舞台国際会議場, International conference

  Poster presentation


• Protein Hydration Dynamics Studied by Broadband Dielectric Spectroscopy

  ○Naoki Yamamoto, Shota Ito, Kaoru Ohta, Atsuo Tamura, Eri Chatani, Hideki Kandori, Keisuke Tominaga

  Japan-Taiwan Medical Spectroscopy International Symposium, Dec. 2016, English, 淡路夢舞台国際会議場, International conference

  Oral presentation


• Investigation of protein aggregation processes leading to amyloid formation

  ○Eri Chatani, Naoki Yamamoto

  Japan-Taiwan Medical Spectroscopy International Symposium, Dec. 2016, English, 淡路夢舞台国際会議場, International conference

  Oral presentation


• Investigating amyloid polymophism by using iodine staining

  Takato Hiramatsu, Seongmin Ha, Yuki Masuda, Mitsuru Yasuda, Mika Ishigaki, Yukihiro Ozaki, Eri Chatani

  Japan-Taiwan Medical Spectroscopy International Symposium, Dec. 2016, English, 淡路夢舞台国際会議場, International conference

  Oral presentation


• 様々な炎症物質を認識するNLRP3-LRRドメインの発現系の確立とその構造的基盤の解明

  山本 良太, 山下 和人, 今村 比呂志, TSUBAKI MOTONARI, CHATANI ERI

  第39回日本分子生物学会年会, Nov. 2016, Japanese, パシフィコ横浜, Domestic conference

  Poster presentation


• Molecular Exploration of Prefibrillar Intermediates That Lead to Formation of Amyloid Fibrils

  〇Eri Chatani, Naoki Yamamoto

  Indo-Japan Joint Seminar on "Frontiers in Molecular Spectroscopy: From Fundamentals to Applications on Material Science and Biology", Nov. 2016, English, Kanpur (Indo), International conference

  [Invited]

  Invited oral presentation


• Investigation of Water Molecular System Dynamics in the Early Stages of Amyloid Formation

  CHATANI ERI, Takato Hiramatsu, Yukari Itakura, Roumiana Tsenkova

  AQUAPHOTOMICS: UNDERSTANDING WATER in BIOLOGY 2nd INTERNATIONAL SYMPOSIUM, Nov. 2016, English, Kobe University, International conference

  [Invited]

  Invited oral presentation


• Investigation of molecular basis underlying the recognition of various inflammatory substances by NLRP3-LRR domain

  ○Ryota Yamamoto, Kazuto Yamashita, Hiroshi Imamura, Motonari Tsubaki, Eri Chatani

  第54回日本生物物理学会年会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• Investigating early steps in amyloid fibril formation

  Eri Chatani

  日中生物物理学会ジョイントシンポジウム：蛋白質設計とバイオテクノロジーへの応用(第54回日本生物物理学会年会), Nov. 2016, English, つくば国際会議場, International conference

  Oral presentation


• Hydration and Thermal-Excitation Effects of Purple Membrane Dynamics Probed by Broadband Dielectric Spectroscopy

  〇Naoki Yamamoto, Shota Ito, Eri Chatani, Hideki Kandori, Keisuke Tominaga

  Indo-Japan Joint Seminar on "Frontiers in Molecular Spectroscopy: From Fundamentals to Applications on Material Science and Biology", Nov. 2016, English, Kanpur (Indo), International conference

  [Invited]

  Invited oral presentation


• Fibrinogen inhibits amyloid fibrillation by stopping at the stage of intermediates

  ○Taiki Akai, Naoki Yamamoto, Eri Chatani

  第54回日本生物物理学会年会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• Effects of Hydration on Protein Dynamics and its Thermal Excitation Studied by Broadband Dielectric Spectroscopy

  〇Naoki Yamamoto, Shota Ito, Hideki Kandori, Kaoru Ohta, Atsuo Tamura, CHATANI ERI, Keisuke Tominaga

  AQUAPHOTOMICS: UNDERSTANDING WATER in BIOLOGY 2nd INTERNATIONAL SYMPOSIUM, Nov. 2016, English, Kobe University, International conference

  [Invited]

  Invited oral presentation


• Effects of hydration and thermal excitation of globular and membrane proteins studied by broadband dielectric spectroscopy

  山本 直樹, 伊藤 奨太, CHATANI ERI, 神取 秀樹, 富永 圭介

  第54回日本生物物理学会年会, Nov. 2016, English, Domestic conference

  Poster presentation


• Effects of amyloid fibrillations on geometries of human insulin as studied by time-resolved EPR spectroscopy

  ○Tomoka Abe, Takashi Tachikawa, Eri Chatani, Paul Zierep, Stefan Weber, Toshifumi Mori, Shinji Saito, Yasuhiro Kobori

  第54回日本生物物理学会年会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• Early detection of amyloid nucleus formation by near infrared spectroscopy

  Takato Hiramatsu, Yukari Itakura, CHATANI ERI, Roumiana Tsenkova

  AQUAPHOTOMICS: UNDERSTANDING WATER in BIOLOGY 2nd INTERNATIONAL SYMPOSIUM, Nov. 2016, English, Kobe University, International conference

  Poster presentation


• 線維前駆中間体に作用点をもつフィブリノーゲンのアミロイド線維化阻害機構

  赤井 大気, YAMAMOTO NAOKI, CHATANI ERI

  新学術領域研究「動的秩序と機能」第３回若手研究会, Oct. 2016, Japanese, 石川県加賀市, Domestic conference

  Poster presentation


• 広帯域誘電分光を用いた紫膜ダイナミクスの観測；サブGHz-THz領域における水和および温度依存性の評価

  YAMAMOTO NAOKI, 伊藤 奨太, CHATANI ERI, 神取 秀樹, TOMINAGA KEISUKE

  第10回分子科学討論会2016神戸, Sep. 2016, Japanese, 神戸ファッションマート, Domestic conference

  Oral presentation


• 広帯域分光による水/ジメチルスルホキシド二成分液体中におけるタンパク質の構造安定性

  飯沼 美紀, YAMAMOTO NAOKI, OHTA KAORU, CHATANI ERI, TOMINAGA KEISUKE

  第10回分子科学討論会, Sep. 2016, Japanese, 神戸ファッションマート, Domestic conference

  Poster presentation


• ラマン分光法を用いたインスリンアミロイド線維形成過程の解析

  森本 佳奈, 石垣 美歌, CHATANI ERI, Roumiana Tsenkova, 尾崎 幸洋

  日本分析化学会第65年会, Sep. 2016, Japanese, 北海道大学工学部, Domestic conference

  Poster presentation


• 電⼦スピン共鳴法を⽤いたヒトインスリンのアミロイド線維化による構造変化の観測

  安倍知花, TACHIKAWA TAKASHI, CHATANI ERI, Till Biskup, Stefan Weber, KOBORI YASUHIRO

  第20回ESRフォーラム研究会 in 神戸, Jul. 2016, Japanese, 神戸大学 瀧川記念学術交流会館, Domestic conference

  Poster presentation


• Mechanism of Aβ1-40 fibrillation by the insertion of a single amino-acid residue

  Kazuto Yamashita, Naoki Yamamoto, Motonari Tsubaki, Eri Chatani

  The 16th Annual Meeting of the Protein Science Society of Japan, Jun. 2016, Japanese, 福岡国際会議場, Domestic conference

  Poster presentation


• The mechanism of the amyloid fibril formation via prefibrillar intermediates -Characterizing early aggregates formed by insulin B chain-

  Naoki Yamamoto, Shoko Tsuhara, Atsuo Tamura, Eri Chatani

  The 16th Annual Meeting of the Protein Science Society of Japan, Jun. 2016, Japanese, 福岡国際会議場, Domestic conference

  Poster presentation


• 広帯域誘電分光法を用いた紫膜のダイナミクスにおける温度および水和依存性の観測

  YAMAMOTO NAOKI, 伊藤 奨太, CHATANI ERI, 神取 秀樹, TOMINAGA KEISUKE

  第43回生体分子科学討論会2016, Jun. 2016, Japanese, 第43回生体分子科学討論会実行委員会, 名古屋大学 野依記念学術交流館, Domestic conference

  Oral presentation


• Coexisting effects of fibrinogen for amyloid fibril formation

  Taiki Akai, Naoki Yamamoto, Eri Chatani

  The 16th Annual Meeting of the Protein Science Society of Japan, Jun. 2016, Japanese, 福岡国際会議場, Domestic conference

  Oral presentation


• Coexisting effects of fibrinogen for amyloid fibril formation

  Taiki Akai, Naoki Yamamoto, Eri Chatani

  The 16th Annual Meeting of the Protein Science Society of Japan, Jun. 2016, Japanese, 福岡国際会議場, Domestic conference

  Poster presentation


• Time-domain EPR observations of changes in protein binding structures induced by the amyloid fibrillation of human insulin

  ○Tomoka Abe, Takashi Tachikawa, Eri Chatani, Till Biskup, Stefan Weber, Yasuhiro Kobori

  4th Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications”, Jun. 2016, English, Awaji Yumebutai International Conference Center, International conference

  Poster presentation


• 線維前駆中間体の観察によるアミロイド線維の伝播およびその発現機構の解明

  CHATANI ERI

  The Japanese Biochemical Society, Kinki Branch, May 2016, Japanese, 神戸薬科大学, Domestic conference

  [Invited]

  Invited oral presentation


• 新規アミロイド構造プローブとしてのヨウ素染色の有用性評価及び染色メカニズムの解析

  平松 貴人, Ha Seongmin, 増田 裕輝, 安田 充, 石垣 美歌, 尾崎 幸洋, CHATANI ERI

  第63回日本生化学会近畿支部例会, May 2016, Japanese, 神戸薬科大学, Domestic conference

  Oral presentation


• ラマン分光法を用いたインスリンアミロイド線維形成過程の解析

  森本 佳奈, 石垣 美歌, CHATANI ERI, Roumiana Tsenkova, 尾崎 幸洋

  第76回分析化学討論会, May 2016, Japanese, 岐阜薬科大学・岐阜大学, Domestic conference

  Poster presentation


• 周波数変調検出方式原子間力顕微鏡を用いたアミロイド線維の液中高分解能観察

  Kataoka Hiroyuki, Kimura Kenjiro, Tyatani Eri, 増田 裕輝, 山下 和人

  日本化学会 第96春季年会, Mar. 2016, Japanese, 同志社大学, Domestic conference

  Oral presentation


• 周波数変調検出方式原子間力顕微鏡を用いたアミロイド線維の液中構造解析

  片岡 寛幸, 木村 建次郎, 茶谷 絵理, 増田 裕輝, 山下 和人

  日本化学会第96春季年会, Mar. 2016, Japanese, 京都, Domestic conference

  Oral presentation


• アミロイド線維形成にみられるタンパク質初期会合メカニズムの解明

  茶谷 絵理

  日本化学会第96春季年会, Mar. 2016, Japanese, 京都, Domestic conference

  [Invited]

  Invited oral presentation


• Establishment of E. coli expression system of Aβ1-40 and its purification without reverse-phase column chromatography

  YAMASHITA KAZUTO, YAMAMOTO NAOKI, TSUBAKI MOTONARI, CHATANI ERI

  BMB2015 (Kobe) 日本生化学会･日本分子生物学会合同年会, Dec. 2015, Japanese, 兵庫県神戸市ポートアイランド, Domestic conference

  Poster presentation


• 近赤外分光法及びアクアフォトミクスによるアミロイド線維核形成の早期検出

  平松 貴人, 板倉 由佳利, 茶谷 絵理, Tsenkova Roumiana

  BMB2015, Dec. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 炎症に関係するNLRP3のLRRドメインの発現系の構築と構造再生の試み

  YAMAMOTO RYOTA, YAMASHITA KAZUTO, IMAMURA HIROSHI, TSUBAKI MOTONARI, CHATANI ERI

  若手フロンティア研究会2015, Dec. 2015, Japanese, 神戸大学研究基盤センター, Domestic conference

  Poster presentation


• アミロイド線維の伝播核形成過程における水の関与性

  茶谷 絵理, 平松 貴人, 板倉 由佳利, 増田 裕輝, Roumiana Tsenkova

  BMB2015, Dec. 2015, Japanese, 神戸, Domestic conference

  [Invited]

  Invited oral presentation


• Construction of an expression system for a ligand-binding domain of an inflammation-related protein NLRP3 and its structural characterization

  YAMAMOTO RYOTA, YAMASHITA KAZUTO, IMAMURA HIROSHI, TSUBAKI MOTONARI, CHATANI ERI

  BMB2015 (Kobe) 日本生化学会･日本分子生物学会合同年会, Dec. 2015, Japanese, 兵庫県神戸市ポートアイランド, Domestic conference

  Poster presentation


• 近赤外分光法を用いたアミロイド線維形成過程における塩の影響に関する研究

  板倉 由佳利, 茶谷 絵理, 平松 貴人, Tsenkova Roumiana

  第31回近赤外フォーラム, Nov. 2015, Japanese, 筑波, Domestic conference

  [Invited]

  Invited oral presentation


• アミロイド線維形成初期に見られる水構造の変化

  茶谷 絵理

  京都大学原子炉実験所「第8回タンパク質の異常凝集とその防御・修復機構に関する研究会」, Nov. 2015, Japanese, 大阪, Domestic conference

  [Invited]

  Invited oral presentation


• アミロイド線維の核形成に伴う水の動態解明

  茶谷 絵理, Tsenkova Roumiana

  第31回近赤外フォーラム, Nov. 2015, Japanese, 筑波, Domestic conference

  Oral presentation


• SAXS study on early aggregation preceding the nucleation of insulin amyloid fibrils

  Eri Chatani, Rintaro Inoue, Hiroshi Imamura, Masaaki Sugiyama, Minoru Kato, Masahide Yamamoto, Koji Nishida, Toshiji Kanaya

  第4回新学術領域研究「動的秩序と機能」国際シンポジウム, Nov. 2015, English, 福岡, International conference

  Poster presentation


• Exploring the early stages of protein association in the formation of amyloid fibrils

  Eri Chatani

  IPR and ANU joint symposium “Protein structure and function”, Nov. 2015, English, Australia, International conference

  [Invited]

  Invited oral presentation


• Elucidating the mechanisms of the amyloid fibril formation via prefibrillar intermediates; a case study on insulin B chain

  Naoki Yamamoto, Shoko Tsuhara, Eri Chatani

  第4回新学術領域研究「動的秩序と機能」国際シンポジウム, Nov. 2015, English, 福岡, International conference

  Poster presentation


• 逆相カラムを用いないAbeta1-40の精製系の確立とこれを用いた線維化機構の解明

  山下 和人, 山本 直樹, 鍔木 基成, 茶谷 絵理

  第2回新学術領域研究「動的秩序と機能」若手研究会, Oct. 2015, Japanese, 愛知, Domestic conference

  Poster presentation


• アミロイド線維形成に与える血液凝固因子フィブリノーゲンの影響

  赤井 大気, 山本 直樹, 茶谷 絵理

  神戸大学サイエンスフロンティア研究発表会, Oct. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• アミロイド形成におけるタンパク質集積メカニズムへの実験的アプローチ

  茶谷 絵理

  第2回新学術領域研究「動的秩序と機能」若手研究会, Oct. 2015, Japanese, 愛知, Domestic conference

  [Invited]

  Invited oral presentation


• Utilizing iodine as a probe for surface structures of amyloid fibrils

  Takato Hiramatsu, Seongmin Ha, Yuki Masuda, Eri Chatani

  第53回日本生物物理学会年会, Oct. 2015, English, 金沢, Domestic conference

  Oral presentation


• The observation of the local structure of the protein in the fibrillation process by using Time-resolved EPR study

  Tomoka Abe, Takashi Tachikawa, Eri Chatani, Yasuhiro Kobori

  第53回日本生物物理学会年会, Oct. 2015, English, 金沢, Domestic conference

  Poster presentation


• Structure characterization of the ligand-binding domain of an inflammation-related protein NLRP3

  ○Ryota Yamamoto, Kazuto Yamashita, Hiroshi Imamura, Motonari Tsubaki, Eri Chatani

  第53回日本生物物理学会年会, Oct. 2015, English, 金沢, Domestic conference

  Poster presentation


• NLRP3タンパク質結合ドメインの発現および構造再生の試み

  山本 良太, 山下 和人, 今村 比呂志, 鍔木 基成, 茶谷 絵理

  第2回新学術領域研究「動的秩序と機能」若手研究会, Oct. 2015, Japanese, 愛知, Domestic conference

  Poster presentation


• Hydration water dynamics of amyloid fibrils studied by terahertz time-domain spectroscopy and dielectric spectroscopy

  Naoki Yamamoto, Keisuke Tominaga, Eri Chatani

  第53回日本生物物理学会年会, Oct. 2015, English, 金沢, Domestic conference

  Oral presentation


• 時間分解EPR法を用いたアミロイド線維を形成するヒトインスリンにおけるアミノ酸残基局所構造の観測

  安倍 知花, TACHIKAWA TAKASHI, CHATANI ERI, KOBORI YASUHIRO

  2015年 光化学討論会, Sep. 2015, Japanese, 大阪市立大学 杉本キャンパス, Domestic conference

  Poster presentation


• 近赤外分光法を用いたアミロイド線維形成過程における構造変化の解明

  TZENKOVA NIKOLOVA Roumiana, 板倉 由佳利, 平松 貴人, CHATANI ERI

  農業環境工学関連5学会2015年合同大会, Sep. 2015, Japanese, 岩手大学, Domestic conference

  Oral presentation


• ラマン分光法を用いたインスリンアミロイド線維形成過程の解析

  森本 佳奈, 石垣 美歌, 茶谷 絵理, Roumiana Tsenkova, 尾崎 幸洋

  日本分析化学会第64年会, Sep. 2015, Japanese, 福岡, Domestic conference

  Poster presentation


• アミロイド線維の核形成におけるタンパク質および水の動態解明

  TZENKOVA NIKOLOVA Roumiana, CHATANI ERI

  第31回近赤外フォーラム, Sep. 2015, Japanese, 筑波大学, Domestic conference

  Oral presentation


• Recent progress in understanding the early stages of generation of amyloid fibrils

  Eri Chatani

  Kwansei Gakuin-Kobe University Joint Symposium: International Symposium on Recent Advanced in Near Infrared Spectroscopy, Sep. 2015, English, 三田, International conference

  [Invited]

  Invited oral presentation


• Femtosecond laser-induced amyloid formation of insulin

  Tsung-Han Liu, Ken-ichi Yuyama, Masayasu Muramatsu, Takato Hiramatsu, Eri Chatani, Teruki Sugiyama, Hiroshi Masuhara

  Annual Meeting on Photochemistry, Sep. 2015, English, 大阪, International conference

  Poster presentation


• Exploring the early stages of generation of amyloid fibrils from the viewpoint of water molecular system dynamics

  Eri Chatani, Yuki Masuda, Yutaro Tsuchisaka, Takato Hiramatsu, Yukari Itakura, Roumiana Tsenkova

  The 15th KIAS Conference on Protein Structure and Function, Sep. 2015, English, Korea, International conference

  [Invited]

  Invited oral presentation


• アミロイド線維構造の面白さ

  茶谷 絵理

  第55回日本生物物理若手の会夏の学校, Aug. 2015, Japanese, 滋賀, Domestic conference

  [Invited]

  Invited oral presentation


• ヨウ素染色によるアミロイド線維構造多形の識別

  平松貴人, Ha SeongMin, 増田 裕輝, CHATANI ERI

  第15回日本蛋白質科学会年会, Jun. 2015, Japanese, あわぎんホール(徳島市）, Domestic conference

  Poster presentation


• ヒトインスリンＢ鎖を用いたアミロイド線維前駆中間体の捕捉による線維形成機構解明

  山本 直樹, 津原祥子, CHATANI ERI

  第15回日本蛋白質科学会年会, Jun. 2015, Japanese, あわぎんホール(徳島市）, Domestic conference

  Poster presentation


• アミロイド線維核の生成メカニズムの解明

  CHATANI ERI

  第15回日本蛋白質科学会年会, Jun. 2015, Japanese, あわぎんホール, Domestic conference

  Oral presentation


• Temperature and Hydration Dependence ofthe Complex Dielectric spectra of proteins at GHz and THz regions obtained by THz Time-domain spectroscopy and dielectric spectroscopy

  Naoki Yamamoto, CHATANI ERI, TAMURA ATSUO, TOMINAGA KEISUKE

  AWEST2015, Jun. 2015, English, 淡路夢舞台国際会議場, International conference

  Invited oral presentation


• Temperature and Hydration Dependence ofthe Complex Dielectric

  CHATANI ERI, TAMURA ATSUO, TOMINAGA KEISUKE

  AWEST2015, Jun. 2015, English, 淡路夢舞台国際会議場, International conference

  Invited oral presentation


• Investigation of molecular mechanism for the generation of amyloidogenic nuclei

  CHATANI ERI

  第15回日本蛋白質科学会年会, Jun. 2015, English, あわぎんホール(徳島市）, Domestic conference

  Invited oral presentation


• ヨウ素染色を用いたアミロイド線維構造多形の識別(ポスター）

  平松 貴人, Ha SeongMin, 増田 裕輝, CHATANI ERI

  第62回日本生化学会近畿支部例会, May 2015, Japanese, 立命館大学びわこ・くさつキャンパス, Domestic conference

  Poster presentation


• ヨウ素染色を用いたアミロイド線維構造多形の識別

  平松 貴人, Ha SeongMin, 増田 裕輝, CHATANI ERI

  第62回日本生化学会近畿支部例会, May 2015, Japanese, 立命館大学びわこ・くさつキャンパス, Domestic conference

  Oral presentation


• インスリンB鎖におけるアミロイド線維前駆中間体の捕捉と構造特徴の解析(ポスター)

  CHATANI ERI, 津原 祥子, 山本 直樹, 増田 裕輝

  第62回日本生化学会近畿支部例会, May 2015, Japanese, 立命館大学びわこ・くさつキャンパス, Domestic conference

  Poster presentation


• インスリンB鎖におけるアミロイド線維前駆中間体の捕捉と構造特徴の解析

  CHATANI ERI, 津原 祥子, 山本 直樹, 増田 裕輝

  第62回日本生化学会近畿支部例会, May 2015, Japanese, 立命館大学びわこ・くさつキャンパス, Domestic conference

  Oral presentation


• アミロイド線維化タンパク質-アントラキノンスルフォン酸イオン複合体の時間分解EPR

  安倍 知花, CHATANI ERI, TACHIKAWA TAKASHI, KOBORI YASUHIRO

  日本化学会第95春季年会2015, Mar. 2015, Japanese, 公益社団法人 日本化学会, 日本大学 理工学部船橋キャンパス, Domestic conference

  Oral presentation


• 前駆中間体を経由したインスリンB鎖のアミロイド線維化機構の解明

  津原 祥子, 増田 裕輝, 山本 直樹, CHATANI ERI

  神戸大学先端融合科学シンポジウム「生体分子のダイナミクスを眺める」, Jan. 2015, Japanese, 神戸大学, Domestic conference

  Poster presentation


• 血液凝固フィブリンの凝集メカニズム

  赤井 大気, CHATANI ERI

  神戸大学先端融合科学シンポジウム「生体分子のダイナミクスを眺める」, Jan. 2015, Japanese, 神戸大学, Domestic conference

  Poster presentation


• ヨウ素染色をプローブとしたアミロイド線維ナノ構造の識別

  平松 貴人, Ha Seongmin, 増田 裕輝, CHATANI ERI

  神戸大学先端融合科学シンポジウム「生体分子のダイナミクスを眺める」, Jan. 2015, Japanese, 神戸大学, Domestic conference

  Poster presentation


• アミロイドβペプチドの線維形成におけるアミノ酸挿入法の効果

  山下 和人, 山本 直樹, 鍔木 基成, CHATANI ERI

  神戸大学先端融合科学シンポジウム「生体分子のダイナミクスを眺める」, Jan. 2015, Japanese, 神戸大学, Domestic conference

  Poster presentation


• Utilizing iodine staining as new probe for distinguish nanostructures of amyloid fibrils

  Takato Hiramatsu, Seongmin Ha, Yuki Masuda, Eri Chatani

  The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions, Jan. 2015, English, 合歓の郷(三重県志摩市), International conference

  Poster presentation


• NLRP3ロイシンリッチリピートの発現系の確立及び機能解析

  山本 良太, 山下 和人, 鍔木 基成, CHATANI ERI

  神戸大学先端融合科学シンポジウム「生体分子のダイナミクスを眺める」, Jan. 2015, Japanese, 神戸大学, Domestic conference

  Poster presentation


• Exploring water molecular system dynamics in the formation of amyloid fibrils using time-resolved near infrared spectroscopy and Aquaphotomics

  Eri Chatani, Yutaro Tsuchisaka, Yuki Masuda, Roumiana Tsenkova

  The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions, Jan. 2015, English, 合歓の郷(三重県志摩市), International conference

  Poster presentation


• Altering nanostructures of insulin amyloid fibrils using zinc ions

  ○Misaki Yokoyama, Yoshito Furuie, Hiroshi Hori, Motonari Tsubaki, Takamasa Nishida, Kazuo Eda, Eri Chatani

  The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions, Jan. 2015, English, 合歓の郷(三重県志摩市), International conference

  Poster presentation


• ヨウ素染色を利用したアミロイド線維ナノ構造の識別

  平松 貴人, Ha SeongMin, 増田 裕輝, 茶谷 絵理

  若手フロンティア研究会２０１４, Dec. 2014, Japanese, Domestic conference

  Poster presentation


• アミロイド伝播核の形成メカニズムの解明

  茶谷 絵理

  京都大学原子炉実験所・専門研究会第7回「タンパク質の異常凝集とその防御・修復機構に関する研究会」, Dec. 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• アミノ酸挿入によるアミロイドβの線維形成への影響

  山下 和人, YAMAMOTO NAOKI, 鍔木 基成, 茶谷 絵理

  若手フロンティア研究会２０１４, Dec. 2014, Japanese, Domestic conference

  Poster presentation


• 近赤外分光法を用いたアミロイド線維形成過程における水分子の結合変化に関する研究

  板倉 由佳利, 平松 貴人, 茶谷 絵理, Tsenkova Roumiana

  第30回記念近赤外フォーラム, Nov. 2014, Japanese, Domestic conference

  Poster presentation


• Understanding early events in protein assembly for the amyloidogenic nucleation

  Eri Chatani

  Japan-Hungary Seminar "Mechanism and regulation of aberrant protein aggregation", Nov. 2014, English, International conference

  [Invited]

  Invited oral presentation


• Association mechanism of protein molecules in the formation of amyloid fibrils

  Eri Chatani

  Indo-Japan Joint Workshop on“Frontiers in Molecular Spectroscopy:Fundamentals and Applications to Materialand Biology”, Nov. 2014, English, International conference

  [Invited]

  Invited oral presentation


• 金属イオンによるアミロイド線維のナノ構造制御

  横山 岬紀, 古家 圭人, 鍔木 基成, HORI HIROSHI, 西田 孝昌, 枝 和男, 茶谷 絵理

  第５回サイエンスフロンティア研究発表会, Oct. 2014, Japanese, Domestic conference

  Poster presentation


• ヨウ素染色によるアミロイド線維構造の識別

  平松 貴人, Ha SeongMin, 増田 裕輝, 茶谷 絵理

  第87回日本生化学会大会, Oct. 2014, Japanese, Domestic conference

  Poster presentation


• ヨウ素染色によるアミロイド線維構造の識別

  平松 貴人, Ha SeongMin, 増田 裕輝, 茶谷 絵理

  第５回サイエンスフロンティア研究発表会, Oct. 2014, Japanese, Domestic conference

  Poster presentation


• アミロイド線維形成過程における前駆中間体の捕捉と観察

  津原 祥子, 増田 裕輝, 茶谷 絵理

  第５回サイエンスフロンティア研究発表会, Oct. 2014, Japanese, Domestic conference

  Poster presentation


• アミノ酸挿入によるアミロイドβペプチドの線維形成への影響

  山下和人, TSUBAKI MOTONARI, CHATANI ERI

  第87回日本生化学会大会, Oct. 2014, Japanese, 京都国際会議場, Domestic conference

  Poster presentation


• アミノ酸挿入によるアミロイドβの線維形成への影響

  山下 和人, 鍔木 基成, 茶谷 絵理

  第５回サイエンスフロンティア研究発表会, Oct. 2014, Japanese, Domestic conference

  Poster presentation


• Exploring water molecular system dynamics in the formation of amyloid fibrils using time-resolved near infrared spectroscopy and aquaphotomics

  Eri Chatani, Yutaro Tsuchisaka, Yuki Masuda, Roumiana Tsenkova

  The 5th Kobe University Brussels European Centre Symposium"AQUAPHOTOMICS: UNDERSTANDING WATER in the BIOLOGICAL WORLD ", Oct. 2014, English, International conference

  Poster presentation


• Effects of single amino-acid insertion on amyloid β fibril formation

  山下 和人, TSUBAKI MOTONARI, CHATANI ERI

  第52回日本生物物理学会年会, Sep. 2014, Japanese, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• Effects of single amino-acid insertion on amyloid β fibril formation

  ○Kazuto Yamashita, Motonari Tsubaki, Eri Chatani

  第52回日本生物物理学会年会, Sep. 2014, English, Domestic conference

  Poster presentation


• Discrimination of amyloid fibril structures by iodine staining

  Takato Hiramatsu, Seongmin Ha, Yuki Masuda, Eri Chatani

  第52回日本生物物理学会年会, Sep. 2014, English, Domestic conference

  Poster presentation


• Near infrared spectral monitoring reveals water molecular system dynamics during the amyloidogenic nucleation

  Eri Chatani, Yutaro Tsuchisaka, Yuki Masuda, Roumiana Tsenkova

  The 27th annual symposium of the protein society, Jul. 2014, English, 米国, International conference

  Poster presentation


• Exploiting an iodine staining as a probe for amyloid fibril structures

  Takato Hiramatsu, Seongmin Ha, Yuki Masuda, Eri Chatani

  The 3rd International Summer Course and Workshop on Single Molecule/Nanoparticle Spectroscopy and ImagingThe 3rd International Summer Course and Workshop onSingle Molecule/Nanoparticle Spectroscopy and Imaging, Jul. 2014, English, 台湾, International conference

  Poster presentation


• Controlling nanostructures of insulin amyloid fibrils using metal ions

  ○Misaki Yokoyama, Yoshito Furuie, Hiroshi Hori, Motonari Tsubaki, Takamasa Nishida, Kazuo Eda, Eri Chatani

  The 27th annual symposium of the protein society, Jul. 2014, English, 米国, International conference

  Poster presentation


• 線維前駆中間体を経由したインスリンB鎖におけるアミロイド線維化の解析

  津原 祥子, 増田 裕輝, CHATANI ERI

  第14回日本蛋白質科学会年会, Jun. 2014, Japanese, ワークピア横浜 / 横浜産貿ホール マリネリア, Domestic conference

  Poster presentation


• 周波数変調検出方式原子間力顕微鏡を用いたアミロイド線維の液中高分解能観察

  片岡 寛幸, 増田 裕輝, 木村 建次郎, 茶谷 絵理

  応用物理学会関西支部平成26年度第1回講演会「プローブ顕微鏡と有機エレクトロニクス～関西若手研究者からの情報発信～」, Jun. 2014, Japanese, Domestic conference

  Poster presentation


• インスリンアミロイドナノ構造の金属イオンによる制御効果

  横山 岬紀, Furuie Yoshito, TSUBAKI MOTONARI, Hori Hiroshi, Nishida Takamasa, Eda Kazuo, Chatani Eri

  第14回日本蛋白質科学会年会, Jun. 2014, Japanese, 日本蛋白質科学会, ワークピア横浜/横浜産貿ホールマリネリア, Domestic conference

  Poster presentation


• Investigation of early association of protein molecules during the amyloidogenic nucleation

  Eri Chatani

  2nd Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications”, Jun. 2014, English, Awaji Island, Hyogo, Japan, International conference

  [Invited]

  Invited oral presentation


• Structural investigation of a prefibrillar intermediate of insulin amyloid fibrils

  Eri Chatani, Hiroshi Imamura, Naoki Yamamoto, Minoru Kato

  The 2nd international symposium of "Dynamical ordering of biomolecular systems for creation of integrated functions", Jan. 2014, English, 京都, International conference

  Poster presentation


• Exploring early association of protein molecules in the amyloid formation

  Eri Chatani

  National Chiao Tung University seminar, Jan. 2014, English, 台湾, International conference

  [Invited]

  Invited oral presentation


• Contribution of water molecules to amyloidogenic nucleation of insulin revealed by salt effects and near infrared spectroscopy

  Yuki Masuda, Eri Chatani

  The 2nd international symposium of "Dynamical ordering of biomolecular systems for creation of integrated functions", Jan. 2014, English, 京都, International conference

  Poster presentation


• 金属イオンによるアミロイドナノ構造の制御

  横山 岬紀, 古家 圭人, 堀 洋, TSUBAKI MOTONARI, 西田 孝昌, EDA KAZUO, CHATANI ERI

  若手フロンティア研究会2013, Dec. 2013, Japanese, 神戸大学研究基盤センター, Domestic conference

  Poster presentation


• アミロイド線維形成過程における前駆中間体の捕捉と観察

  津原 祥子, 増田 裕輝, 茶谷 絵理

  若手フロンティア研究会2013, Dec. 2013, Japanese, 神戸大学研究基盤センター, Domestic conference

  Poster presentation


• Polymorphism of insulin amyloid fibrils induced by the coordination of metal ions

  横山 岬紀, FURUIE YOSHITO, HORI HIROSHI, TSUBAKI MOTONARI, CHATANI ERI

  第51回日本生物物理学会年会, Oct. 2013, English, 京都国際会館, Domestic conference

  Poster presentation


• Polymorphism of insulin amyloid fibrils induced by the coordination of metal ions

  MASUDA YUKI, FURUIE YOSHITO, HORI HIROSHI, TSUBAKI MOTONARI, CHATANI ERI

  第51回日本生物物理学会年会, Oct. 2013, English, 京都, Domestic conference

  Poster presentation


• Observation of various types of amyloid prefibrillar intermediates of insulin B chain

  TSUHARA SHOKO, CHATANI ERI

  第51回日本生物物理学会年会, Oct. 2013, English, 京都, Domestic conference

  Poster presentation


• Exploring roles of water molecules on amyloid fibrillation by salt effects and Near Infrared spectroscopy

  MASUDA YUKI, TSUCHISAKA YUTARO, TSENKOVA ROUMIANA, CHATANI ERI

  第51回日本生物物理学会年会, Oct. 2013, English, 京都, Domestic conference

  Poster presentation


• Stepwise organization of insulin amyloid fibrils via a prefibrillar intermediate

  CHATANI ERI, IMAMURA HIROSHI, YAMAMOTO NAOKI, KATO MINORU

  27th annual symposium of the protein society, Jul. 2013, English, Boston, USA, International conference

  Poster presentation


• Role of water on the formation of insulin amyloid fibrils

  MASUDA YUKI, CHATANI ERI

  27th annual symposium of the protein society, Jul. 2013, English, Boston, USA, International conference

  [Invited]

  Poster presentation


• 金属イオン配位によるインスリンアミロイド線維の多形誘導効果

  横山 岬紀, 古家 圭人, 堀 洋, TSUBAKI MOTONARI, CHATANI ERI

  第13回日本蛋白質科学会年会, Jun. 2013, Japanese, 日本蛋白質科学会, 鳥取大学, Domestic conference

  Poster presentation


• 塩効果より予測されたアミロイド線維化に対する水の重要性

  MASUDA YUKI, CHATANI ERI

  第13回日本蛋白質科学会年会, Jun. 2013, Japanese, 鳥取大学, Domestic conference

  Poster presentation


• インスリンB鎖に見られる多様なアミロイド線維前駆中間体の観察

  TSUHARA SHOKO, CHATANI ERI

  第13回日本蛋白質科学会年会, Jun. 2013, Japanese, 鳥取大学, Domestic conference

  Poster presentation


• Stepwise organization of insulin amyloid structure via a prefibrillar intermediate

  CHATANI ERI

  蛋白質研究所セミナー「アジア・太平洋地域における蛋白質科学の連携を目指して‐フォールディングとミスフォールディング研究の新展開‐」, Jun. 2013, English, 大阪大学, International conference

  Invited oral presentation


• 近赤外分光法を用いたインスリンアミロイド形成過程の非破壊モニタリング

  土坂 祐太郎, 増田 裕輝, CHATANI ERI, ツェンコヴァ ルミャナ

  第28回近赤外フォーラム, Mar. 2013, Japanese, 近赤外フォーラム, 那覇, Domestic conference

  Poster presentation


• アミロイド線維形成機構と制御の解析

  CHATANI Eri

  横浜国立大学大学院研究セミナー：機能性生体分子の構造生物学的研究, Dec. 2012, Japanese, 横浜国立大, 横浜, Domestic conference

  [Invited]

  Invited oral presentation


• アミロイド線維形成に及ぼすイオンの作用機構

  増田 裕輝, CHATANI Eri

  第85回日本生化学学会年会, Dec. 2012, Japanese, 日本生化学学会, 博多, Domestic conference

  Poster presentation


• アミロイド線維形成に及ぼすイオンの作用機構

  増田 裕輝, CHATANI Eri

  神戸大学研究基盤センター「 若手フロンティア研究会2012」, Dec. 2012, Japanese, 神戸大学, 神戸, Domestic conference

  Poster presentation


• 小角X線散乱によるアミロイド線維形成の初期会合プロセスの解析

  CHATANI Eri, 井上 倫太郎, 西田 幸次, 金谷 利治, 山本 雅英

  第50回日本生物物理学会年会, Sep. 2012, Japanese, 日本生物物理学会, 名古屋, Domestic conference

  Poster presentation


• インスリンアミロイド線維前駆中間体の捕捉と構造解析

  CHATANI Eri, 今村 比呂志, 山本 直樹, 加藤 稔

  第12回日本蛋白質科学会年会, Jun. 2012, Japanese, 日本蛋白質科学会, 名古屋, Domestic conference

  Poster presentation


• インスリンアミロイド線維形成に対するイオンの作用機構

  増田 裕輝, CHATANI Eri

  第12回日本蛋白質科学会年会, Jun. 2012, Japanese, 日本蛋白質科学会, 名古屋, Domestic conference

  Poster presentation


• 小角X線散乱によるアミロイド線維の構造解析

  井上 倫太郎, CHATANI Eri, 西田 幸次, 金谷 利治, 山本 雅英

  第61回高分子学会年次大会, May 2012, Japanese, 日本高分子学会, 横浜, Domestic conference

  Poster presentation


• アミロイド線維の形成機構

  CHATANI Eri

  奈良女子大学酵素クラブ第３回セミナー, May 2012, Japanese, 奈良女子大学, 奈良, Domestic conference

  [Invited]

  Invited oral presentation


• 1E1424 Investigation of initial assembling process of fibrillation by small angle X-ray scattering(Proteins: Property I,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

  Chatani Eri, Inoue Rintaro, Nishida Koji, Kanaya Toshiji, Yamamoto Masahide

  Seibutsu Butsuri, 2012, English, The Biophysical Society of Japan General Incorporated Association


• インスリンアミロイド線維形成に及ぼすカチオンとアニオンの影響

  増田 裕輝, CHATANI Eri

  神戸大学先端融合科学シンポジウム：蛋白質アセンブリ‐会合・超分子化・凝集, Jan. 2012, Japanese, 神戸大学, 神戸, Domestic conference

  Poster presentation


• アミロイド線維伸長反応の分子機構

  CHATANI Eri

  神戸大学先端融合科学シンポジウム：蛋白質アセンブリ‐会合・超分子化・凝集, Jan. 2012, Japanese, 神戸大学, 神戸, Domestic conference

  Invited oral presentation


• アミロイドβ19-34断片と変異体モノマーのターン構造形成能とアミロイド線維形成能

  山置 佑大, CHATANI Eri, 今村 比呂志, 高尾 英佑, 加藤 稔

  神戸大学先端融合科学シンポジウム：蛋白質アセンブリ‐会合・超分子化・凝集, Jan. 2012, Japanese, 神戸大学, 神戸, Domestic conference

  Poster presentation


• FT-IRと蛍光によるαシヌクレインのアミロイド線維形成の解析と圧力解離

  秦 和澄, 秋田 まゆみ, 位田 雅俊, CHATANI Eri, 加藤 稔

  神戸大学先端融合科学シンポジウム：蛋白質アセンブリ‐会合・超分子化・凝集, Jan. 2012, Japanese, 神戸大学, 神戸, Domestic conference

  Poster presentation


• The relationship between the abilities of amyloid fibril formation and turn structure formation: study of amyloid-β peptide fragment Aβ 19-34 and its mutants with high turn propensity

  Yudai Yamaoki, CHATANI Eri, Hiroshi Imamura, Eisuke Takao, Minoru Kato

  6th International Meeting on Biomolecules under Pressure, Dec. 2011, English, Shiga, Japan, International conference

  Oral presentation


• タンパク質凝集と食品：アミロイド線維の形成機構

  CHATANI Eri

  シンポジウム：新たな機能性食品開発へ向けた取り組み‐異分野融合による機能性食品のイノベーション‐, Sep. 2011, Japanese, 立命館大学, 神戸, Domestic conference

  [Invited]

  Invited oral presentation


• The polymorphic structures of insulin amyloid fibrils formed at natural pH

  Takahiro Shibata, CHATANI Eri, Hiroshi Imamura, Eisuke Takao, Minoru Kato

  第49回日本生物物理学会年会, Sep. 2011, English, 日本生物物理学会, 姫路, Domestic conference

  Oral presentation


• Mechanism of amyloid fibril formation as revealed by small angle X-ray scattering

  Rintaro Inoue, CHATANI Eri, Hiroshi Imamura, ToshijiKanaya, Koji Nishida, Minoru Kato, SonoSasaki, Hiroki Ogawa, Hiroyasu Masunaga, Masahide Yamamoto

  第49回日本生物物理学会年会, Sep. 2011, English, 日本生物物理学会, 姫路, Domestic conference

  Oral presentation


• Atomic force microscopy study of the stepwise organization of insulin amyloid fibrils

  Eisuke Takao, CHATANI Eri, Hiroshi Imamura, Minoru Kato

  第49回日本生物物理学会年会, Sep. 2011, English, 日本生物物理学会, 姫路, Domestic conference

  Oral presentation


• Kinetic intermediate of β2-microglobulin fibril elongation probed by tryptophan fluorescence spectroscopy and H/D exchange-NMR

  CHATANI Eri, Tsuyoshi Konuma, Reina Ohnishi, Masanori Yagi, Kazumasa Sakurai, Takahisa Ikegami, Hironobu Naiki, Yuji Goto

  Conference "Amyloid Fibrils, Prions and Precursors: Molecules for Targeted Intervention", Aug. 2011, English, Germany, International conference

  Oral presentation


• 超音波を用いたアミロイド線維の微細化・均一化の試み

  CHATANI Eri

  第12回若手NMR研究会, Jun. 2011, Japanese, 若手NMR研究会, 滋賀, Domestic conference

  [Invited]

  Invited oral presentation


• 伸長反応中間体の観察によるアミロイド構造伝播機構の解明

  CHATANI Eri, 小沼 剛, 大西 玲奈, 八木 正典, 櫻井 一正, 池上 貴久, 内木 宏延, 後藤 祐児

  第11回日本蛋白質科学会年会, Jun. 2011, Japanese, 日本蛋白質科学会, 大阪, Domestic conference

  [Invited]

  Invited oral presentation


• 異常凝集の構造生物学－β2ミクログロブリンアミロイド伸長中間体の構造解析－

  CHATANI Eri

  大阪大学蛋白質研究所セミナー：蛋白質異常凝集の原理と制, Apr. 2011, Japanese, 大阪大学, 大阪, Domestic conference

  [Invited]

  Invited oral presentation

• 日本分子生物学会


• 日本生化学会


• 日本生物物理学会


• 日本蛋白質科学会

• 液液相分離中アミロイド生成の定量的解析法の確立

  福山 真央, 大橋 祐美子, 粕谷 素洋, 白木 賢太郎, 茶谷 絵理, 冨田 峻介

  日本学術振興会, 科学研究費助成事業 挑戦的研究(開拓), 挑戦的研究(開拓), 東北大学, 30 Jun. 2022 - 31 Mar. 2026


• アミロイド核生成を誘起するタンパク質集合動態の解明

  茶谷 絵理, 大橋 祐美子

  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 神戸大学, 01 Apr. 2020 - 31 Mar. 2024


• 病態診断を指向したアミロイド構造多形の簡便かつ迅速な評価法の構築

  茶谷 絵理

  日本学術振興会, 科学研究費助成事業 挑戦的研究(萌芽), 挑戦的研究(萌芽), 神戸大学, 30 Jul. 2020 - 31 Mar. 2023


• Mechanism and regulation of protein aggregation in complicated cellular systems

  後藤 祐児, 茶谷 絵理

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Osaka University, 30 Jun. 2017 - 31 Mar. 2022

  昨年度に引き続き、以下の３つの研究を並行して行うと共に、領域内のメンバーと連携して研究を進めた。 (1)試験管内夾雑モデル系の構築と凝集分子機構：αシヌクレインの等電点pH、低イオン強度条件下でアミロイド線維について、蛍光分光法、NMRを用いた解析を進めた。その結果、等電点pHを含むさまざまな条件において、蛋白質の溶解度が低下することによりアミロイド線維ができることの一般性を示した。さらに生体膜表面でpHが低下することによってアミロイド線維形成が促進することを示唆した。本結果は、論文発表した。 (2)アンフィンゼンのドグマとアミロイド線維形成の関係：これまで、β２ミクログロブリンを中心として、塩濃度や温度の効果を研究してきた。その結果、従来可逆的に進行すると思われていた熱変性（アンフィンゼンのドグマ）も、撹拌などのアジテーションによって過飽和を解消することによって不可逆となり、アミロイド線維の形成に至ることを明らかにした。さらにアンフィンゼン型の可逆的な変性とアミロイド線維形成を定式化して結合することができた。本結果は論文発表した。 (3)β２ミクログロブリンの原子構造の解明：蛋白質研究所の固体NMRの研究グループと、固体NMRを用いて、β2ミクログロブリンのアミロイド線維に構造解析を行ってきた。クライオ電顕の研究グループとも共同研究することにより、原子レベルの構造解析を進めた。 (4)アミロイド形成促進因子：アミロイド形成を促進する化合物（例えばポリリン酸）に注目した研究を進めた。透析患者や一般検体の血清を用いて、アミロイド線維形成を加速するHANABI装置によるβ2ミクログロブリンのアミロイド線維形成反応を調べたところ、透析患者においては明らかな促進することを見出した。これを促進する生体環境を解析した。


• Biochemical study of maintenance DNA methylation regulated by histone modification(s)

  末武 勲, 茶谷 絵理

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Koshien University, 01 Apr. 2018 - 31 Mar. 2021

  哺乳類で、遺伝子発現抑制に関与するとされる「DNAメチル化」は、発生の過程でそのパターンがゲノム上に確定されると、細胞分裂を介して安定に維持される。この安定な維持には、Dnmt1という酵素が必要であることが明らかにされている。しかしながら、試験管内で2本鎖DNAのうち片方の鎖をメチル化したDNA(ヘミメチル化DNA)をDnmt1の基質にして反応させると、100%の効率でメチル化しえないことが分かっている。そのため、ゲノム上のDNAメチル化を細胞分裂を超えて安定に維持するには、Dnmt1活性を促進する、または活性を安定化させる機構があると考えられていた。 数年前に、私は、核タンパク質であるヒストンH3がユビキチン化されるとDnmt1と結合し、Dnmt1の構造変化をひき起こして、Dnmt1の活性中心を開くことで、Dnmt1活性を促進することを報告しているがIshiyama, Suetakeら、Mol Cell,2017）、その促進機構は不明な点があった。 本年度は、私は、生化学的に、ユビキチン化ヒストンH3（H3Ub)が、Dnmt1の「DNAの上を滑って連続的にメチル化する能力」を亢進することを見出した(Mishima Suetakeら Genes to Cells, 2019)。さらに私は、これまでにDnmt1活性が、他分子の部分領域（Uhrf1のSRAドメイン）により促進されることを報告しているので（Berkyurek, Suetakeら, JBC, 2014 ）、SRAとユビキチン化H3を同時添加することで、それらが相加的にDnmt1活性促進をすることも見出した(Mishima Suetakeら Genes to Cells, 2019)。
  つまり、Dnmt1活性の新制御機構を見出し、DNAメチル化の安定維持機構の理解を深めることができた。


• 線維前駆中間体の解析を基軸としたアミロイド線維の伝播性および毒性の構造基盤の解明

  茶谷 絵理

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2020, Principal investigator

  Competitive research funding


• アミロイド核の出現に先立つタンパク質初期集合および秩序化プロセスの解明

  茶谷 絵理

  科学研究費補助金／新学術領域研究, Apr. 2016 - Mar. 2018, Principal investigator

  Competitive research funding


• アミロイド伝播核生成相におけるタンパク質分子の集合・秩序化メカニズムの解明

  茶谷 絵理

  科学研究費補助金／新学術領域研究, Apr. 2014 - Mar. 2016, Principal investigator

  Competitive research funding


• アミロイド線維の伝播性およびその発現メカニズムに関する構造的研究

  茶谷 絵理

  学術研究助成基金助成金／基盤研究(C), Apr. 2013 - Mar. 2016, Principal investigator

  Competitive research funding


• 線維前駆中間体の構造解析によるアミロイドーシス伝藩機構の解明

  茶谷 絵理

  学術研究助成基金助成金／若手研究(B), 2011 - 2012, Principal investigator

  Competitive research funding


• Structural investigation of the fibrillation intermediate of β_2-microglobulin by H/D exchange-NMR

  CHATANI Eri

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Ritsumeikan University, Apr. 2008 - Mar. 2010, Principal investigator

  Protein misfolding occasionally leads to the formation of supramolecular assemblies known as amyloid fibrils, the deposition of which is associated with over 30 degenerative diseases. One of the most unique and essential properties of amyloid fibrils is their template-dependent growth, which underlies the propagation of pathology of amyloidoses. We have investigated a monomer-fibril complex of β_2-microglobulin formed transiently during the fibril growth by using Trp fluorescence and hydrogen/deuterium (H/D) exchange-NMR methods.


• タンパク質の構造形成原理:折りたたみに関わる疎水性アミノ酸残基の特性の解析

  茶谷 絵理

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, Grant-in-Aid for JSPS Fellows, Osaka University, Apr. 2003 - Mar. 2006, Principal investigator

  本課題は、タンパク質の立体構造構築原理を明らかにし、さらに、場合によってはアミロイド線維のようにまったく異なる構造をとりうる現象の解明を目標にしている。平成15-16年度にわたり、ヒト由来β_2ミクログロブリンの形成するアミロイド線維に対する加圧効果を詳細に解析した。その結果、アミロイド線維は通常の球状タンパク質に比べて側鎖の相互作用の貢献が少なく、パッキングの程度が低い可能性が判明したので、本年度は、アミロイド線維が形成される過程をさまざまな圧力下で観察し、遷移状態の体積に関する情報を収集した。複数のアミロイド線維構造に対して活性化体積を算出したところ、従来の球状タンパク質の折りたたみ反応とは異なり、アミロイド線維構造形成過程にも側鎖の相互作用の貢献が少ない可能性が示唆された。さらに、高圧下ではアミロイド線維の適応現象も観察され、アミロイドーシスの伝播機構に関する知見を得ることもできた。アミロイド線維研究に対する圧力因子の適用例はまだ少ないが、今後本研究を発展させることによって、アミロイド線維に特有な構造形成機構の解明が期待される。 この研究では、これまでと同様に大腸菌を宿主とした発現系を用いてのβ_2ミクログロブリンの大量発現、精製を頻繁に繰り返し、培地、精製に用いるカラムのような消耗品の購入と、高圧力装置に関する消耗品を購入した。さらに、研究打ち合わせのための出張と、高圧力シンポジウム(THPPS2005)での研究発表のためにフランスへの渡航も行った。


• ウシ膵臓リボヌクレアーゼAの大量発現系の構築とこれを用いる構造生物学的研究

  茶谷 絵理

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, Grant-in-Aid for JSPS Fellows, Kyoto University, Apr. 1999 - Mar. 2002, Principal investigator