Research activity information

• Aug. 2007 日本植物細胞分子生物学会, 日本植物細胞分子生物学会技術賞, バイオテクノロジーを利用したフキならびにナスの育種

  岩本 嗣, 中曽根 渡, 平井 正志, 近江戸 伸子, 福井 希一


• Aug. 2007 国際牧草分子生物学会, 第5回国際牧草分子生物学会ポスター賞, Chromosome map analysis of red clover

  近江戸 伸子


• Mar. 2002 日本育種学会, 日本育種学会奨励賞, 超微細蛍光in situハイブリダイゼーション法の開発

  近江戸 伸子


• Jan. 2001 財団法人日本染色体学会, 染色体学会賞, 高感度可視化法による植物染色体の分子細胞学的研究

  近江戸 伸子

• Repeatome landscapes and cytogenetics of hortensias provide a framework to trace Hydrangea evolution and domestication.

  Sara Ishiguro, Shota Taniguchi, Nicola Schmidt, Matthias Jost, Stefan Wanke, Tony Heitkam, Nobuko Ohmido

  BACKGROUND AND AIMS: Ornamental hortensias are bred from a reservoir of over 200 species in the genus Hydrangea s.l. (Hydrangeaceae), and are valued in gardens, households and landscapes across the globe. The phenotypic diversity of hortensia cultivars, hybrids and wild relatives is mirrored by their genomic variation, with differences in genome size, base chromosome numbers and ploidy level. We aim to understand the genomic and chromosomal basis of hortensia genome variation. Therefore, we analysed six hortensias with different origins and chromosomal setups for repeatome divergence, the genome fraction with the highest sequence turnover. This holds information from the hortensias' evolutionary paths and can guide breeding initiatives. METHODS: We compiled a hortensia genotype panel representing members of the sections Macrophyllae, Hydrangea, Asperae and Heteromallae and reconstructed a plastome-based phylogenetic hypothesis as the evolutionary basis for all our analyses. We comprehensively characterized the repeatomes by whole-genome sequencing and comparative repeat clustering. Major tandem repeats were localized by multicolour FISH. KEY RESULTS: The Hydrangea species show differing repeat profiles reflecting their separation into the two major Hydrangea clades: diploid Hydrangea species from Japan show a conserved repeat profile, distinguishing them from Japanese polyploids as well as Chinese and American hortensias. These results are in line with plastome-based phylogenies. The presence of specific repeats indicates that H. paniculata was not polyploidized directly from the common ancestor of Japanese Hydrangea species, but evolved from a distinct progenitor. Major satellite DNAs were detected over all H. macrophylla chromosomes. CONCLUSIONS: Repeat composition among the Hydrangea species varies in congruence with their origins and phylogeny. Identified species-specific satDNAs may be used as cytogenetic markers to identify Hydrangea species and cultivars, and to infer parental species of old Hydrangea varieties. This repeatome and cytogenetics information helps to expand the genetic toolbox for tracing hortensia evolution and guiding future hortensia breeding.

  Corresponding, Feb. 2025, Annals of botany, 135(3) (3), 549 - 564, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Allopolyploidy enhances survival advantages for urban environments in the native plant genus Commelina

  Hina Shimomai, Nakata Taichi, Koki R Katsuhara, Seiji Kato, Atushi Ushimaru, Nobuko Ohmido

  Abstract Background and Aims Urbanization-induced environmental changes affect the geographical distribution of natural plant species. This study focused on how polyploidization, a dynamic genome change, influences the survival and distribution of Commelina communis (Cc) and its subspecies C. communis f. ciliata (Ccfc), which have variable chromosome numbers (e.g. Cc, 2n = 88 for Cc; Ccfc, 2n = 46 for Ccfc). The aim was to investigate polyploidization effects on natural plant distribution in urban environments. Methods The geographical distribution across urban–rural gradients was investigated at a total of 218 sites in Japan. Stomata size and density were measured and compared between Cc and Ccfc. Flow cytometry determined genome size and polyploidy. Chromosome karyotyping was performed using the genomic in situ hybridization (GISH) method. Key Results Urban areas were exclusively dominated by Cc, while Cc and Ccfc coexisted in rural areas. Cc had larger and fewer stomata and a genome size more than twice that of Ccfc. GISH results indicated that Cc possesses Ccfc and another unknown genome, suggesting allopolyploidy. Conclusions Our results show that the ploidy difference affects the geographical distribution, stomata traits and genome size between two distinct taxa in the genus Commelina, C. communis as a neo-tetraploid and C. communis f. ciliata, the diploid. Cc is an allopolyploid and is therefore not only polyploidy but also has an additional genome that provides new sets of genes and alleles, contributing to Cc having enhanced survival potentials in urban environments compared with Ccfc. This is the first investigation to clarify the distribution difference related to urban environments, the difference in stomata traits and genome size, and to study chromosome composition in Commelina species.

  Corresponding, Oxford University Press (OUP), Aug. 2024, Annals of Botany

  [Refereed]

  Scientific journal


• Cytogenetic analysis of progeny from interspecific hybridization between Jatropha curcas and Jatropha integerrima using the GISH method

  Matiya Changjalern, Nobuko Omido, Penjit Srinophakun, Vipa Hongtrakul

  Science Society of Thailand, Apr. 2024, ScienceAsia, 50(3) (3), 1 - 1, English

  Scientific journal


• Localization and quantitative distribution of a chromatin structural protein Topoisomerase II on plant chromosome using HVTEM and UHVTEM.

  Channarong Sartsanga, Rinyaporn Phengchat, Toshiyuki Wako, Kiichi Fukui, Nobuko Ohmido

  Topoisomerase II (TopoII) is an essential structural protein of the metaphase chromosome. It maintains the axial compaction of chromosomes during metaphase. It is localized at the axial region of chromosomes and accumulates at the centromeric region in metaphase chromosomes. However, little is known about TopoII localization and distribution in plant chromosomes, except for several publications. We used high voltage transmission electron microscopy (HVTEM) and ultra-high voltage transmission electron microscopy (UHVTEM) in conjunction with immunogold labeling and visualization techniques to detect TopoII and investigate its localization, alignment, and density on the barley chromosome at 1.4 nm scale. We found that HVTEM and UHVTEM combined with immunogold labeling is suitable for the detection of structural proteins, including a single molecule of TopoII. This is because the average size of the gold particles for TopoII visualization after silver enhancement is 8.9 ± 3.9 nm, which is well detected. We found that 31,005 TopoII molecules are distributed along the barley chromosomes in an unspecific pattern at the chromosome arms and accumulate specifically at the nucleolus organizer regions (NORs) and centromeric region. The TopoII density were 1.32-fold, 1.58-fold, and 1.36-fold at the terminal region, at the NORs, and the centromeric region, respectively. The findings of TopoII localization in this study support the multiple reported functions of TopoII in the barley metaphase chromosome.

  Corresponding, Apr. 2024, Micron (Oxford, England : 1993), 179, 103596 - 103596, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Habitat diversification associated with urban development has a little effect on genetic structure in the annual native plant Commelina communis in an East Asian megacity

  Nakata Taichi, Naoyuki Nakahama, Nobuko Ohmido, Atushi Ushimaru

  Abstract Urban development greatly alters the natural and semi‐natural habitats of native plants. Urbanisation results in a range of diverse habitats including remnant agricultural lands, urban parks, and roadside habitats. This habitat diversity often promotes trait divergence within urban areas. However, the mechanisms by which diverse urban habitats influence the population genetic structure of individual plant species remain poorly understood. We investigated the effects of urbanisation on genetic diversity and structure within 24 Commelina communis populations across diverse habitat types (rural agricultural land, urban agricultural land, urban park land, and urban roadsides) within the Kyoto–Osaka–Kobe megacity in Japan. We conducted multiplexed inter‐simple sequence repeat genotyping to compare genetic diversity among populations in different habitats. We also examined the correlation between Nei's genetic distance and geographic and environmental distances and performed principal coordinate analysis (PCoA) to evaluate genetic differentiation among urban habitats. There were no significant differences in genetic diversity indices between urban and rural populations and among urban habitat types. Although we detected no isolation‐by‐distance structure in population pairs of the same habitat type and in those of different habitats, the difference in surrounding landscape facilitated genetic differentiation not only between urban and rural habitats but also between different urban habitats. PCoA revealed no clear genetic differentiation among rural and urban habitat populations. Our findings indicate that the establishment of diverse habitat types through urbanisation has no and little impact on genetic diversity and structure, respectively, in C. communis, likely due to its high selfing rate and ability to adapt to urban conditions.

  Wiley, Feb. 2024, Ecology and Evolution, 14(2) (2)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 科学技術の社会問題としてのゲノム編集を題材とした小学生向け教育プログラムの評価：リスク重視傾向の変化過程の検討

  口羽駿平, 山口悦司, 坂本美紀, 山本智一, 原愛佳, 近江戸伸子, 村山留美子, 俣野源晃, 澁野哲

  Dec. 2023, 日本科学教育学会研究会研究報告, 38(2) (2), 219 - 222, Japanese

  Symposium


• Ultrastructure of Javaen barb fish Systomus orphoides Valenciennes, 1842 spermatozoa (Cypriniformes: Cyprinidae) by electron microscopes.

  Sri Widiyanti Rahayu Hilia, Abinawanto, Astari Dwiranti, Anom Bowolaksono, Retno Lestari, Fadhilah, Anang Hari Kristanto, Nobuko Ohmido

  Javaen barb fish Systomus orphoides Valenciennes, 1842 (Cypriniformes: Cyprinidae) is a freshwater fish whose population is declining and threatened with extinction. In this study, the ultrastructure of spermatozoa of Javaen barb fish (S. orphoides) was studied using transmission and scanning electron microscopy. The spermatozoa of S. orphoides are relatively simple cells composed of a spherical head, a short midpiece, and a flagellum, as in most Cyprinidae. The ultrastructure is characterized by the absence of acrosome, the total length of spermatozoa is 27.16 ± 4.5 μm, and the head has spherical with a length of 1.84 ± 0.10 μm and width of 1.55 ± 0.15 μm containing a nucleus, midpiece region containing the proximal and distal centrioles and mitochondria. Two or three mitochondria surrounding the axoneme (with a 9 + 2 microtubular pattern). Ultrastructural analyses by SEM and TEM of Javaen barb fish spermatozoa cells are very consistent with those of Cyprinidae. This study provides the ultrastructure information of S. orphoides spermatozoa in the Cyprinidae family this research could be useful in increasing reproductive efficiency and further prevent the extinction of this species.

  Nov. 2023, Microscopy research and technique, 86(11) (11), 1411 - 1415, English, International magazine

  [Refereed]

  Scientific journal


• CRISPR imaging reveals chromatin fluctuation at the centromere region related to cellular senescence.

  Hideaki Takata, Yumena Masuda, Nobuko Ohmido

  The human genome is spatially and temporally organized in the nucleus as chromatin, and the dynamic structure of chromatin is closely related to genome functions. Cellular senescence characterized by an irreversible arrest of proliferation is accompanied by chromatin reorganisation in the nucleus during senescence. However, chromatin dynamics in chromatin reorganisation is poorly understood. Here, we report chromatin dynamics at the centromere region during senescence in cultured human cell lines using live imaging based on the clustered regularly interspaced short palindromic repeat/dCas9 system. The repetitive sequence at the centromere region, alpha-satellite DNA, was predominantly detected on chromosomes 1, 12, and 19. Centromeric chromatin formed irregular-shaped domains with high fluctuation in cells undergoing 5'-aza-2'-deoxycytidine-induced senescence. Our findings suggest that the increased fluctuation of the chromatin structure facilitates centromere disorganisation during cellular senescence.

  Sep. 2023, Scientific reports, 13(1) (1), 14609 - 14609, English, International magazine

  [Refereed]

  Scientific journal


• Author Correction: Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines.

  Yikun Liu, Yeng Mun Liaw, Chee How Teo, Petr Cápal, Naoki Wada, Kiichi Fukui, Jaroslav Doležel, Nobuko Ohmido

  Corresponding, May 2023, Scientific reports, 13(1) (1), 8487 - 8487, English, International magazine

  [Refereed]


• Chromatin Immunostaining of Plant Nuclei.

  Nobuko Ohmido, Aqwin Polosoro

  Recent evidence has demonstrated that specific epigenetic changes are also related to plant growth and development. Immunostaining enables the detection and characterization of chromatin modification, e.g., histone H4 acetylation (H4K5ac), histone H3 methylation (H3K4me2 and H3K9me2), and DNA methylation (5mC) with unique and specific patterns in plant tissues. Here we describe experimental procedures to determine the histone H3 methylation (H3K4me2 and H3K9me2) patterns in 3D-chromatin in whole roots tissue and 2D-chromatin in single nuclei in rice. To analyze both iron and salinity treatments, we show how to test for changes to the epigenetic chromatin landscape using heterochromatin (H3K9me2) and euchromatin (H3K4me) markers for chromatin immunostaining, especially in the proximal meristem region. To elucidate the epigenetic impact of environmental stress and external plant growth regulators, we demonstrate how to apply a combination of salinity, auxin, and abscisic acid treatments. The results of these experiments provide insights into the epigenetic landscape during rice root growth and development.

  2023, Methods in molecular biology (Clifton, N.J.), 2672, 233 - 244, English, International magazine

  [Refereed]

  Scientific journal


• Higher-order structure of barley chromosomes observed by electron tomography

  Misa Hayashida, Channarong Sartsanga, Rinyaporn Phengchat, Marek Malac, Ken Harada, Tetsuya Akashi, Kiichi Fukui, Nobuko Ohmido

  Sep. 2022, Micron, 160, 103328 - 103328, English

  [Refereed]

  Scientific journal


• Applications of image analysis in plant chromosome and chromatin structure study

  Nobuko Ohmido, Astari Dwiranti, Seiji Kato, Kiichi Fukui

  Lead, Apr. 2022, Quantitative Biology, 10(3) (3), 226 - 238, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Water Spectral Patterns Reveals Similarities and Differences in Rice Germination and Induced Degenerated Callus Development.

  Zoltan Kovacs, Jelena Muncan, Nobuko Ohmido, George Bazar, Roumiana Tsenkova

  In vivo monitoring of rice (Oryza sativa L.) seed germination and seedling growth under general conditions in closed Petri dishes containing agar base medium at room temperature (temperature = 24.5 ± 1 °C, relative humidity = 76 ± 7% (average ± standard deviation)), and induced degenerated callus formation with plant growth regulator, were performed using short-wavelength near-infrared spectroscopy and aquaphotomics over A period of 26 days. The results of spectral analysis suggest changes in water absorbances due to the production of common metabolites, as well as increases in biomass and the sizes of the samples. Quantitative models built to predict the day of the development provided better accuracy for rice seedlings growth compared to callus formation. Eight common water bands were identified as presenting prominent changes in the absorbance pattern. The water matrix of only rice seedlings showed three developmental stages: firstly expressing a predominantly weakly hydrogen-bonded state, then a more strongly hydrogen-bonded state, and then, again, a weakly hydrogen-bonded state at the end. In rice callus induction and proliferation, no similar change in water absorbance pattern was observed. The presented findings indicate the potential of aquaphotomics for the in vivo detection of degeneration in cell development.

  Sep. 2021, Plants (Basel, Switzerland), 10(9) (9), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Calcium ion significance on the maintenance of barley (Hordeum vulgare) chromosome compaction.

  Aldi Yazri Siregar, Channarong Sartsanga, Fendi Sofyan Arifudin, Rinyaporn Phengchat, Andi Salamah, Nobuko Ohmido, Kiichi Fukui, Astari Dwiranti

  Cations, especially calcium ions (Ca2+), is one of the major factors responsible for the chromosome higher-order structure formation. The effects of cations on the human chromosomes have already been evaluated, however, whether the presence of similar effects on plant chromosomes has not been reported to date. Thus, in this study, we investigated the role of Ca2+ on the barley (Hordeum vulgare L.) chromosome structure. Barley chromosomes were isolated from the meristematic tissue within the germinated roots. The roots were subjected to enzymatic treatment, fixed, and drop on the cover glass to spread the chromosomes out. Some chromosomes were treated with BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) to chelate Ca2+. Chromosome samples were then observed by fluorescence microscopy and scanning electron microscopy (SEM). The disperse structure of the chromosome was observed after BAPTA treatment. Chromosomes showed less condensed structure due to Ca2+ chelation. The high-resolution of SEM provided a more detailed visualization of chromosome ultrastructure under different calcium ion conditions. This study revealed the calcium ion effect on chromosome structure is important regardless of the organisms, suggesting a similar mechanism of chromosome condensation through humans and plants.

  Jun. 2021, Micron (Oxford, England : 1993), 145, 103046 - 103046, English, International magazine

  [Refereed]

  Scientific journal


• Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a Human-Arabidopsis Hybrid Cell Line.

  YengMun Liaw, Yikun Liu, CheeHow Teo, Petr Cápal, Naoki Wada, Kiichi Fukui, Jaroslav Doležel, Nobuko Ohmido

  Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human-Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes.

  Corresponding, May 2021, International journal of molecular sciences, 22(11) (11), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines.

  Yikun Liu, Yeng Mun Liaw, Chee How Teo, Petr Cápal, Naoki Wada, Kiichi Fukui, Jaroslav Doležel, Nobuko Ohmido

  Although plants and animals are evolutionarily distant, the structure and function of their chromosomes are largely conserved. This allowed the establishment of a human-Arabidopsis hybrid cell line in which a neo-chromosome was formed by insertion of segments of Arabidopsis chromosomes into human chromosome 15. We used this unique system to investigate how the introgressed part of a plant genome was maintained in human genetic background. The analysis of the neo-chromosome in 60- and 300-day-old cell cultures by next-generation sequencing and molecular cytogenetics suggested its origin by fusion of DNA fragments of different sizes from Arabidopsis chromosomes 2, 3, 4, and 5, which were randomly intermingled rather than joined end-to-end. The neo-chromosome harbored Arabidopsis centromeric repeats and terminal human telomeres. Arabidopsis centromere wasn't found to be functional. Most of the introgressed Arabidopsis DNA was eliminated during the culture, and the Arabidopsis genome in 300-day-old culture showed significant variation in copy number as compared with the copy number variation in the 60-day-old culture. Amplified Arabidopsis centromere DNA and satellite repeats were localized at particular loci and some fragments were inserted into various positions of human chromosome. Neo-chromosome reorganization and behavior in somatic cell hybrids between the plant and animal kingdoms are discussed.

  Corresponding, Mar. 2021, Scientific reports, 11(1) (1), 7160 - 7160, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy.

  Channarong Sartsanga, Rinyaporn Phengchat, Kiichi Fukui, Toshiyuki Wako, Nobuko Ohmido

  The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction of chromatin fibers has been a topic of controversy since the discovery of chromosomes in the 19th century. Although chromatin fibers were first identified using electron microscopy, the chromatin fibers on the surface of chromosome structures in plants remain unclear due to shrinking and breaking caused by prior chromosome isolation or preparation with alcohol and acid fixation, and critical point drying occurred into dehydration and denatured chromosomal proteins. This study aimed to develop a high-quality procedure for the isolation and preparation of plant chromosomes, maintaining the native chromosome structure, to elucidate the organization of chromatin fibers on the surface of plant chromosomes by electron microscopy. A simple technique to isolate intact barley (Hordeum vulgare) chromosomes with a high yield was developed, allowing chromosomes to be observed with a high-resolution scanning ion microscopy and helium ion microscopy (HIM) imaging technology, based on a scanning helium ion beam. HIM images from the surface chromatin fibers were analyzed to determine the size and alignment of the chromatin fibers. The unit size of the chromatin fibers was 11.6 ± 3.5 nm and was closely aligned to the chromatin network model. Our findings indicate that compacting the surface structure of barley via a chromatin network and observation via HIM are powerful tools for investigating the structure of chromatin.

  Corresponding, Mar. 2021, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 29(1) (1), 81 - 94, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Higher-Order Structure of Human Chromosomes Observed by Electron Diffraction and Electron Tomography.

  Misa Hayashida, Rinyaporn Phengchat, Marek Malac, Ken Harada, Tetsuya Akashi, Nobuko Ohmido, Kiichi Fukui

  It is well known that two DNA molecules are wrapped around histone octamers and folded together to form a single chromosome. However, the nucleosome fiber folding within a chromosome remains an enigma, and the higher-order structure of chromosomes also is not understood. In this study, we employed electron diffraction which provides a noninvasive analysis to characterize the internal structure of chromosomes. The results revealed the presence of structures with 100–200 nm periodic features directionally perpendicular to the chromosome axis in unlabeled isolated human chromosomes. We also visualized the 100–200 nm periodic features perpendicular to the chromosome axis in an isolated chromosome whose DNA molecules were specifically labeled with OsO4 using electron tomography in 300 keV and 1 MeV transmission electron microscopes.

  Feb. 2021, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27(1) (1), 149 - 155, English, International magazine

  [Refereed]

  Scientific journal


• Application of the Chromosome Image Analyzing System (CHIAS) for Straightening Cation-treated Bent Chromosomes.

  Astari Dwiranti, Fendi Sofyan Arifudin, Hideaki Takata, Nobuko Ohmido, Kiichi Fukui

  Divalent cations, mainly calcium and magnesium ions, are known to play a major role in the maintenance of chromosomes. The depletion of both ions using ethylenediaminetetraacetic acid (EDTA) results in a bent chromosome structure with extended arms and dispersed chromatin fibers. The importance of divalent cations for the maintenance of chromosome structure has been reported previously; nevertheless, previous studies were limited to qualitative data only. Straightening the bent image of the chromosome would provide quantitative data. Thus, this study aimed to evaluate the effects of cation depletion by the application of the Chromosome Image Analyzing System (CHIAS) to straighten bent chromosomes. Human HeLa chromosomes were treated with EDTA as a known chelating agent in order to investigate the importance of divalent cations on the maintenance of chromosome structure. Chromosomes were stained and directly observed with a fluorescence microscope. Images were then analyzed using CHIAS. The results revealed that EDTA-treated chromosomes showed longer arms than those without EDTA treatment, and most of them tended to bend-out. By straightening the image using CHIAS, the bent chromosomes were successfully straightened. The average lengths of the chromosomes treated with and without EDTA were 4.97 and 0.96 μm, respectively. These results signify the advantages of CHIAS for chromosome analysis and highlight the fundamental effects of cations on chromosome condensation.

  Nov. 2020, Microscopy research and technique, 83(11) (11), 1411 - 1416, English, International magazine

  [Refereed]

  Scientific journal


• Higher-order Structure of Human Chromosomes Observed by Electron Tomography and Electron Diffraction

  Misa Hayashida, Rinyaporn Phengchat, Darren Homeniuk, Marek Malac, Ken Harada, Tetsuya Akashi, Nobuko Ohmido, Kiichi Fukui

  Cambridge University Press (CUP), Jul. 2020, Microscopy and Microanalysis, 1 - 5

  [Refereed]

  Scientific journal


• Evolution and Diversity of the Wild Rice Oryza officinalis Complex, across Continents, Genome Types, and Ploidy Levels.

  Matt Shenton, Masaaki Kobayashi, Shin Terashima, Hajime Ohyanagi, Dario Copetti, Tania Hernández-Hernández, Jianwei Zhang, Nobuko Ohmido, Masahiro Fujita, Atsushi Toyoda, Hiroshi Ikawa, Asao Fujiyama, Hiroyasu Furuumi, Toshie Miyabayashi, Takahiko Kubo, David Kudrna, Rod Wing, Kentaro Yano, Ken-Ichi Nonomura, Yutaka Sato, Nori Kurata

  The Oryza officinalis complex is the largest species group in Oryza, with more than nine species from four continents, and is a tertiary gene pool that can be exploited in breeding programs for the improvement of cultivated rice. Most diploid and tetraploid members of this group have a C genome. Using a new reference C genome for the diploid species O. officinalis, and draft genomes for two other C genome diploid species Oryza eichingeri and Oryza rhizomatis, we examine the influence of transposable elements on genome structure and provide a detailed phylogeny and evolutionary history of the Oryza C genomes. The O. officinalis genome is 1.6 times larger than the A genome of cultivated Oryza sativa, mostly due to proliferation of Gypsy type long-terminal repeat transposable elements, but overall syntenic relationships are maintained with other Oryza genomes (A, B, and F). Draft genome assemblies of the two other C genome diploid species, Oryza eichingeri and Oryza rhizomatis, and short-read resequencing of a series of other C genome species and accessions reveal that after the divergence of the C genome progenitor, there was still a substantial degree of variation within the C genome species through proliferation and loss of both DNA and long-terminal repeat transposable elements. We provide a detailed phylogeny and evolutionary history of the Oryza C genomes and a genomic resource for the exploitation of the Oryza tertiary gene pool.

  Apr. 2020, Genome biology and evolution, 12(4) (4), 413 - 428, English, International magazine

  [Refereed]

  Scientific journal


• Global epigenetic changes of histone modification under environmental stresses in rice root.

  Aqwin Polosoro, Wening Enggarini, Nobuko Ohmido

  Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulated by chromatin reconfiguration and histone modification. Several types of histone modifications are dramatically transformed in stress-responsive gene regions under drought stress conditions. Environmental stresses also cause the root apical meristem (RAM) region to decelerate root growth. In this study, we investigated how quantitative changes in epigenetic markers in this region influence rice morphology and physiology. Both iron and salinity treatments changed the epigenetic landscape from euchromatic to heterochromatic according to heterochromatin (H3K9me2) and euchromatin (H3K4me) markers, especially in the proximal meristem region. Moreover, supplementation with external abscisic acid (ABA) was able to mimic the effect of environmental stresses on global epigenetic changes. In contrast, the addition of external auxin (IAA) to rice under saline conditions affected heterochromatin formation without influencing euchromatin transformation. Chromatin dynamics is therefore believed to be directly connected to plant growth regulator signaling. We discuss insights into the role of plant growth regulators: ABA and IAA, peroxide signaling, and their effects on the global epigenetic change of histone modification under abiotic stresses.

  Corresponding, Dec. 2019, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 27(4) (4), 287 - 298, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 3D observation of chromosome scaffold structure using a 360° electron tomography sample holder.

  Rinyaporn Phengchat, Misa Hayashida, Nobuko Ohmido, Darren Homeniuk, Kiichi Fukui

  The chromosome scaffold is considered to be a key structure of the mitotic chromosome. It plays a vital role in chromosome condensation, shaping the X-shaped structure of the mitotic chromosome, and also provides flexibility for chromosome movement during cell division. However, it remains to be elucidated how the chromosome scaffold organizes the mitotic chromosome and how it supports shaping the structure of the chromosome during metaphase. Here we present a new technique that enables the observation of the chromosome scaffold structure in metaphase chromosomes from any direction, by transferring an isolated chromosome to a 360° rotational holder for electron tomography (ET). The chromosome was stained with immunogold-labeled condensin complex, one of the major chromosome scaffold proteins and then observed in three dimensions using ET. Using the locations of gold nanoparticles to visualize the underlying structure, the tomograms we obtained reveal the patterns of chromosome scaffold organization, which appears to consist of a helical structure that serves to organize chromatin loops into the metaphase chromosome.

  Nov. 2019, Micron (Oxford, England : 1993), 126, 102736 - 102736, English, International magazine

  [Refereed]

  Scientific journal


• Cytogenetic and agronomic characterization of intergeneric hybrids between Saccharum spp. hybrid and Erianthus arundinaceus.

  Babil Pachakkil, Yoshifumi Terajima, Nobuko Ohmido, Masumi Ebina, Shin Irei, Hisayoshi Hayashi, Hiroko Takagi

  In sugarcane (Saccharum spp. hybrid) breeding, introgression of useful genes via intergeneric hybridization is a powerful strategy for improving the crop productivity. Erianthus arundinaceus shows great potential in terms of useful traits; however, little is known about the cytogenetic and agronomic characteristics of intergeneric hybrids between these two species. Here, we examine the cytogenetic and agronomic characteristics, and relationships between the two in intergeneric F1 hybrids between modern sugarcane cultivar and E. arundinaceus identified by amplification of 5S rDNA markers and morphological characteristics. The nuclear DNA content of the hybrids varied from 6.07 to 8.94 pg/2C, with intra-clonal variation in DNA content and 5S rDNA sites. Genomic in situ hybridization revealed 53 to 82 chromosomes in the hybrids, with 53 to 56 derived from sugarcane and 1 to 29 from E. arundinaceus. There were significant positive correlations between the number of E. arundinaceus chromosomes and dry matter yield, millable stalk weight, single stalk weight, and stalk diameter, but not sucrose content, reducing sugar content, sucrose/reducing sugar ratio or fiber content. This detailed information on intergeneric F1 hybrids between modern sugarcane cultivar and E. arundinaceus will contribute to effective utilization of E. arundinaceus in sugarcane breeding.

  Springer Nature Publishing AG, Feb. 2019, Scientific reports, 9(1) (1), 1748 - 1748, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Molecular insights into the non-recombining nature of the spinach male-determining region.

  Tomohiro Kudoh, Mitsuhiko Takahashi, Takayuki Osabe, Atsushi Toyoda, Hideki Hirakawa, Yutaka Suzuki, Nobuko Ohmido, Yasuyuki Onodera

  Spinach (Spinacia oleracea L.) is a dioecious plant with male heterogametic sex determination and homomorphic sex chromosomes (XY). The dioecism is utilized for producing commercial hybrid seeds, and hence understanding the molecular-genetic basis of the species' sex determining locus is an important issue for spinach breeding. In this study, seven dominant DNA markers were shown to completely co-segregate with the male-determining gene in segregating spinach populations comprising > 1500 plants. In addition, these seven dominant DNA markers were completely associated with the male-determining gene in over 100 spinach germplasm accessions and cultivars. These observations suggest that, in spinach, a Y-chromosomal region around the male-determining locus does not (or almost not) recombine with a counterpart region on the X chromosome. Using five of the seven DNA markers, five bacterial artificial chromosome (BAC) clone contigs with a total length of approximately 690 kbp were constructed. Full sequencing of six representative BAC clones (total insert length 504 kbp) from the five contigs and a transcriptome analysis by RNA-seq revealed that the Y-chromosomal region around the male-determining locus contains large amounts of repetitive elements, suggesting that the region might be poor in gene content. Most of the repeats found in this region are novel Ty1-copia-like and its derivative elements that accumulate predominantly in heterochromatic regions. Our findings may provide valuable insight into spinach genome structure and clues for future research into the evolution of the sex determining locus.

  Springer, Apr. 2018, Molecular genetics and genomics : MGG, 293(2) (2), 557 - 568, English, International magazine

  [Refereed]

  Scientific journal


• Development of a quantitative pachytene chromosome map and its unification with somatic chromosome and linkage maps of rice (Oryza sativa L.).

  Nobuko Ohmido, Aiko Iwata, Seiji Kato, Toshiyuki Wako, Kiichi Fukui

  A quantitative pachytene chromosome map of rice (Oryza sativa L.) was developed using imaging methods. The map depicts not only distribution patterns of chromomeres specific to pachytene chromosomes, but also the higher order information of chromosomal structures, such as heterochromatin (condensed regions), euchromatin (decondensed regions), the primary constrictions (centromeres), and the secondary constriction (nucleolar organizing regions, NOR). These features were image analyzed and quantitatively mapped onto the map by Chromosome Image Analyzing System ver. 4.0 (CHIAS IV). Correlation between H3K9me2, an epigenetic marker and formation and/or maintenance of heterochromatin, thus was, clearly visualized. Then the pachytene chromosome map was unified with the existing somatic chromosome and linkage maps by physically mapping common DNA markers among them, such as a rice A genome specific tandem repeat sequence (TrsA), 5S and 45S ribosomal RNA genes, five bacterial artificial chromosome (BAC) clones, four P1 bacteriophage artificial chromosome (PAC) clones using multicolor fluorescence in situ hybridization (FISH). Detailed comparison between the locations of the DNA probes on the pachytene chromosomes using multicolor FISH, and the linkage map enabled determination of the chromosome number and short/long arms of individual pachytene chromosomes using the chromosome number and arm assignment designated for the linkage map. As a result, the quantitative pachytene chromosome map was unified with two other major rice chromosome maps representing somatic prometaphase chromosomes and genetic linkages. In conclusion, the unification of the three rice maps serves as an indispensable basic information, not only for an in-depth comparison between genetic and chromosomal data, but also for practical breeding programs.

  PLOS, 2018, PloS one, 13(4) (4), e0195710, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Chromosome instability of allopolyploid resynthesized Brassica napus.

  OHMIDO NOBUKO, Kanako Ueda, Kouei Fujii

  Allopolyploid resynthesized Brassica napus (AACC, 2n = 38) was produced by cross-hybridization between B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18) for new vegetative crop breeding. Many studies have provided evidences for the phenotype instability and close relationship between A and C genome in the amphidiploid resynthesized B. napus cultivars. In fact, a new B. napus

  Oct. 2016, Chromosome Science, 18, 79 - 84, English

  [Refereed]

  International conference proceedings


• Image Analysis of DNA Fiber and Nucleus in Plants.

  Nobuko Ohmido, Toshiyuki Wako, Seiji Kato, Kiichi Fukui

  Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play important roles in the biological systems. Visualization and image analysis methods are useful techniques in the analyses of the detailed structure and function of extended DNA fibers (EDFs) and interphase nuclei. The EDF is the highest in the spatial resolving power to reveal genome structure and it can be used for physical mapping, especially for closely located genes and tandemly repeated sequences. One the other hand, analyzing nuclear DNA and proteins would reveal nuclear structure and functions. In this chapter, we describe the image analysis protocol for quantitatively analyzing different types of plant genome, EDFs and interphase nuclei.

  2016, Methods in molecular biology (Clifton, N.J.), 1469, 171 - 80, English, International magazine

  [Refereed]

  Scientific journal


• Evidence for a Common Origin of Homomorphic and Heteromorphic Sex Chromosomes in Distinct Spinacia Species.

  Satoshi Fujito, Satoshi Takahata, Reimi Suzuki, Yoichiro Hoshino, Nobuko Ohmido, Yasuyuki Onodera

  The dioecious genus Spinacia is thought to include two wild relatives (S. turkestanica Ilj. and S. tetrandra Stev.) of cultivated spinach (S. oleracea L.). In this study, nuclear and chloroplast sequences from 21 accessions of Spinacia germplasm and six spinach cultivars or lines were subjected to phylogenetic analysis to define the relationships among the three species. Maximum-likelihood sequence analysis suggested that the Spinacia plant samples could be classified into two monophyletic groups (Group 1 and Group 2): Group 1 consisted of all accessions, cultivars, and lines of S. oleracea L. and S. turkestanica Ilj. and two of five S. tetrandra Stev. accessions, whereas Group 2 was composed of the three remaining S. tetrandra Stev. accessions. By using flow cytometry, we detected a distinct difference in nuclear genome size between the groups. Group 2 also was characterized by a sexual dimorphism in inflorescence structure, which was not observed in Group 1. Interspecific crosses between the groups produced hybrids with drastically reduced pollen fertility and showed that the male is the heterogametic sex (XY) in Group 2, as is the case in S. oleracea L. (Group 1). Cytogenetic and DNA marker analyses suggested that Group 1 and Group 2 have homomorphic and heteromorphic sex chromosome pairs (XY), respectively, and that the sex chromosome pairs of the two groups evolved from a common ancestral pair. Our data suggest that the Spinacia genus may serve as a good model for investigation of evolutionary mechanisms underlying the emergence of heteromorphic sex chromosome pairs from ancestral homomorphic pairs.

  Jun. 2015, G3 (Bethesda, Md.), 5(8) (8), 1663 - 73, English, International magazine

  [Refereed]

  Scientific journal


• Cell differentiation and development in Arabidopsis are associated with changes in histone dynamics at the single-cell level.

  Stefanie Rosa, Vardis Ntoukakis, Nobuko Ohmido, Ali Pendle, Rita Abranches, Peter Shaw

  Dec. 2014, The Plant cell, 26(12) (12), 4821 - 33, English, International magazine

  [Refereed]

  Scientific journal


• Chromosome interior observation by focused ion beam/scanning electron microscopy (FIB/SEM) using ionic liquid technique.

  Tohru Hamano, Astari Dwiranti, Kohei Kaneyoshi, Shota Fukuda, Reo Kometani, Masayuki Nakao, Hideaki Takata, Susumu Uchiyama, Nobuko Ohmido, Kiichi Fukui

  Attempts to elucidate chromosome structure have long remained elusive. Electron microscopy is useful for chromosome structure research because of its high resolution and magnification. However, biological samples such as chromosomes need to be subjected to various preparation steps, including dehydration, drying, and metal/carbon coating, which may induce shrinkage and artifacts. The ionic liquid technique has recently been developed and it enables sample preparation without dehydration, drying, or coating, providing a sample that is closer to the native condition. Concurrently, focused ion beam/scanning electron microscopy (FIB/SEM) has been developed, allowing the investigation and direct analysis of chromosome interiors. In this study, we investigated chromosome interiors by FIB/SEM using plant and human chromosomes prepared by the ionic liquid technique. As a result, two types of chromosomes, with and without cavities, were visualized, both for barley and human chromosomes prepared by critical point drying. However, chromosome interiors were revealed only as a solid structure, lacking cavities, when prepared by the ionic liquid technique. Our results suggest that the existence and size of cavities depend on the preparation procedures. We conclude that combination of the ionic liquid technique and FIB/SEM is a powerful tool for chromosome study.

  Oct. 2014, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 20(5) (5), 1340 - 7, English, International magazine

  [Refereed]

  Scientific journal


• Chromosome Interior Observation by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) Using Ionic Liquid Technique.

  OHMIDO NOBUKO, Hamano T, Dwiranti A, Kaneyoshi K, Fukuda S, Kometani R, Nakao M, Takata H, Uchiyama S, Fukui K

  Jun. 2014, Microsc Microanal., 10, 1 - 8, English

  [Refereed]

  Scientific journal


• Karyotyping using FISH and Chromosome Image Analysis System IV.

  Nobuko Ohmido, Akane Shimoura, Seiji Kato, Sachiko Isobe, Satoshi Tabata

  In this study, kudzu (Pueraria lobata Ohwi), a new biomass plant widely grown in Japan, was investigated by chromosome karyotyping. Four strains of Japanese kudzu were examined, and all were found to contain 22 chromosomes. The 5S and 45S rDNA genes, which contain fundamental repeat sequences used in karyotyping, were determined by fluorescence in situ hybridization (FISH). Our

  Society of Chromosome Research, Feb. 2014, Chromosome Science, 16(1+2) (1+2), 17 - 21, English

  [Refereed]

  Scientific journal


• Identification and Characterization of Novel Gypsy-Type Retrotransposons in a Biodiesel Crop, Jatropha curcas L

  Atefeh Alipour, Joyce A. Cartagena, Suguru Tsuchimoto, Hiroe Sakai, Nobuko Ohmido, Kiichi Fukui

  Springer New York LLC, 2014, Plant Molecular Biology Reporter, 32(4) (4), 923 - 930, English

  [Refereed]

  Scientific journal


• Structural characterization of copia-type retrotransposons leads to insights into the marker development in a biofuel crop, Jatropha curcas L.

  Atefeh Alipour, Suguru Tsuchimoto, Hiroe Sakai, Nobuko Ohmido, Kiichi Fukui

  BACKGROUND: Recently, Jatropha curcas L. has attracted worldwide attention for its potential as a source of biodiesel. However, most DNA markers have demonstrated high levels of genetic similarity among and within jatropha populations around the globe. Despite promising features of copia-type retrotransposons as ideal genetic tools for gene tagging, mutagenesis, and marker-assisted selection, they have not been characterized in the jatropha genome yet. Here, we examined the diversity, evolution, and genome-wide organization of copia-type retrotransposons in the Asian, African, and Mesoamerican accessions of jatropha, then introduced a retrotransposon-based marker for this biofuel crop. RESULTS: In total, 157 PCR fragments that were amplified using the degenerate primers for the reverse transcriptase (RT) domain of copia-type retroelements were sequenced and aligned to construct the neighbor-joining tree. Phylogenetic analysis demonstrated that isolated copia RT sequences were classified into ten families, which were then grouped into three lineages. An in-depth study of the jatropha genome for the RT sequences of each family led to the characterization of full consensus sequences of the jatropha copia-type families. Estimated copy numbers of target sequences were largely different among families, as was presence of genes within 5 kb flanking regions for each family. Five copia-type families were as appealing candidates for the development of DNA marker systems. A candidate marker from family Jc7 was particularly capable of detecting genetic variation among different jatropha accessions. Fluorescence in situ hybridization (FISH) to metaphase chromosomes reveals that copia-type retrotransposons are scattered across chromosomes mainly located in the distal part regions. CONCLUSION: This is the first report on genome-wide analysis and the cytogenetic mapping of copia-type retrotransposons of jatropha, leading to the discovery of families bearing high potential as DNA markers. Distinct dynamics of individual copia-type families, feasibility of a retrotransposon-based insertion polymorphism marker system in examining genetic variability, and approaches for the development of breeding strategies in jatropha using copia-type retrotransposons are discussed.

  Sep. 2013, Biotechnology for biofuels, 6(1) (1), 129 - 129, English, International magazine

  [Refereed]

  Scientific journal


• Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization.

  Stefanie Rosa, Filomena De Lucia, Joshua S Mylne, Danling Zhu, Nobuko Ohmido, Ali Pendle, Naohiro Kato, Peter Shaw, Caroline Dean

  Vernalization, the promotion of flowering by cold, involves Polycomb-mediated epigenetic silencing of FLOWERING LOCUS C (FLC). Cold progressively promotes cell-autonomous switching to a silenced state. Here, we used live-cell imaging of FLC-lacO to monitor changes in nuclear organization during vernalization. FLC-lacO alleles physically cluster during the cold and generally remain so after plants are returned to warm. Clustering is dependent on the Polycomb trans-factors necessary for establishment of the FLC silenced state but not on LIKE HETEROCHROMATIN PROTEIN 1, which functions to maintain silencing. These data support the view that physical clustering may be a common feature of Polycomb-mediated epigenetic switching mechanisms.

  Sep. 2013, Genes & development, 27(17) (17), 1845 - 50, English, International magazine

  [Refereed]

  Scientific journal


• Advances in Imaging Methods on Plant Chromosomes.

  Wako, T, Kato, S, Ohmido, N, Fukui, K

  Jul. 2013, Plant Image Analysis, English

  [Refereed]


• Molecular cytogenetics of rice and its wild relatives

  Aiko Iwata, Dongying Gao, Nobuko Ohmido, Scott A. Jackson

  Springer New York, Jan. 2013, Genetics and Genomics of Rice, 71 - 79, English

  [Refereed]

  In book


• Integration of Linkage and Chromosome Maps of Red Clover (Trifolium pratense L.)

  R. Kataoka, M. Hara, S. Kato, S. Isobe, S. Sato, S. Tabata, N. Ohmido

  2012, CYTOGENETIC AND GENOME RESEARCH, 137(1) (1), 60 - 69, English

  [Refereed]

  Scientific journal


• Stable progeny production of the amphidiploid resynthesized Brassica napus cv. Hanakkori, a newly bred vegetable

  K. Fujii, N. Ohmido

  Dec. 2011, THEORETICAL AND APPLIED GENETICS, 123(8) (8), 1433 - 1443, English

  [Refereed]

  Scientific journal


• Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.

  Shusei Sato, Hideki Hirakawa, Sachiko Isobe, Eigo Fukai, Akiko Watanabe, Midori Kato, Kumiko Kawashima, Chiharu Minami, Akiko Muraki, Naomi Nakazaki, Chika Takahashi, Shinobu Nakayama, Yoshie Kishida, Mitsuyo Kohara, Manabu Yamada, Hisano Tsuruoka, Shigemi Sasamoto, Satoshi Tabata, Tomoyuki Aizu, Atsushi Toyoda, Tadasu Shin-i, Yohei Minakuchi, Yuji Kohara, Asao Fujiyama, Suguru Tsuchimoto, Shin'ichiro Kajiyama, Eri Makigano, Nobuko Ohmido, Nakako Shibagaki, Joyce A Cartagena, Naoki Wada, Tsutomu Kohinata, Alipour Atefeh, Shota Yuasa, Sachihiro Matsunaga, Kiichi Fukui

  The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.

  Feb. 2011, DNA research : an international journal for rapid publication of reports on genes and genomes, 18(1) (1), 65 - 76, English, International magazine

  [Refereed]

  Scientific journal


• Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes.

  Nobuko Ohmido, Akiko Ishimaru, Seiji Kato, Shusei Sato, Satoshi Tabata, Kiichi Fukui

  To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major tandem repeat DNAs (LjTR1 and LjTR2), two major retroelements (LjRE1 and LjRE2), and 27 transformation-competent artificial chromosome clones were physically localized on Lotus japonicus (Miyakojima MG-20, 2n = 12) chromosomes. The distributions of heterochromatin and euchromatin along six chromosomes were compared based on the linkage map. Distortion between the recombination frequencies and physical chromosomal distance was recognized where the centromeric heterochromatic regions and constitutive heterochromatin are composed of the highest copy tandem repeat LjTR1 on the interstitial specific regions. Our study shows that the heterochromatin are composed of the specific repeated sequences, and the discrepancy between the recombination frequency and cytological information detected in L. japonicus chromosomes is due to the heterochromatin.

  Feb. 2010, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 18(2) (2), 287 - 99, English, International magazine

  [Refereed]

  Scientific journal


• Physical mapping of ribosomal DNA genes on Jatropha curcas chromosomes by multicolor FISH.

  N. Ohmido, M. Witkowska, J. Cartagena, N. Shibagaki, S. Kajiyama, K. Fukui

  Feb. 2010, Cytologia, vol.74 pp. 133-139, 133 - 139, English

  [Refereed]

  Scientific journal


• Image analysis of small plant chromosomes by using an improved system, CHIAS IV

  KATO Seiji, OHMIDO Nobuko, HARA Masaki, KATAOKA Ryouhei, FUKUI Kiichi

  Small plant chromosomes show uneven condensation during the prometaphase stage in mitosis. Recently, an improved chromosome image analyzing system (CHIAS IV) has been released for measurement of the condensation patterns (CPs) in fluorescence images. CHIAS IV is based on the multiplatform public-domain image-processing software, ImageJ, which was developed by the National Institutes of Health, US. In this study, we used CHIAS IV to perform image analysis of the fluorescence patterns of somatic prometaphase chromosomes of the leguminous plant, red clover (Trifolium pratense L., 2n = 2x = 14). We produced a quantitative chromosome map of red clover by combining the qualitative-analysis results obtained using fluorescence in situ hybridization (FISH) mapping with the results of the CHIAS IV image-analysis method, thereby clearly demonstrating the effectiveness and utility of this software as an analytical tool for genetic and genomic studies.

  THE SOCIETY OF CHROMOSOME RESEARCH, Jul. 2009, Chromosome Science, 12(3) (3), 43 - 50, English


• Anti-peptide antibodies for examining the conformation, molecular assembly, and localization of an intracellular protein, ribosormal protein S6, in vivo.

  N. Ohmido, M. Nakagawa, K. Ishikawa, S. Uchiyama, K. Fukui, T. Azuma

  Nov. 2008, J. Biochem., vol. 143, pp. 325-332, English

  [Refereed]

  Scientific journal


• Genome structure of the legume, Lotus japonicus.

  Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko, Erika Asamizu, Tomohiko Kato, Mitsuteru Nakao, Shigemi Sasamoto, Akiko Watanabe, Akiko Ono, Kumiko Kawashima, Tsunakazu Fujishiro, Midori Katoh, Mitsuyo Kohara, Yoshie Kishida, Chiharu Minami, Shinobu Nakayama, Naomi Nakazaki, Yoshimi Shimizu, Sayaka Shinpo, Chika Takahashi, Tsuyuko Wada, Manabu Yamada, Nobuko Ohmido, Makoto Hayashi, Kiichi Fukui, Tomoya Baba, Tomoko Nakamichi, Hirotada Mori, Satoshi Tabata

  The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10,951 complete and 19,848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.

  Aug. 2008, DNA research : an international journal for rapid publication of reports on genes and genomes, 15(4) (4), 227 - 39, English, International magazine

  [Refereed]

  Scientific journal


• Anti-peptide antibodies for examining the conformation, molecular assembly and localization of an intracellular protein, ribosomal protein S6, in vivo.

  Masatoshi Nakagawa, Nobuko Ohmido, Katsumi Ishikawa, Susumu Uchiyama, Kiichi Fukui, Takachika Azuma

  Ribosomal protein S6 (rpS6) is known to relate to cell proliferation. Our recent proteome analysis of human metaphase chromosomes revealed the enrichment of rpS6 during mitosis. Here, structure, localization and molecular assembly in vitro and in vivo of a human rpS6, were examined using antibodies (Abs) prepared by immunizing rabbits with synthetic peptides. Five peptides, Ser6-Asp20 (S6-1), Ile52-Gly66 (S6-2), Asp103-Gly117 (S6-3), Asn146-Lys160 (S6-4) and Arg178-Ile192 (S6-5) were chosen as epitopes of human rpS6. These peptides except for S6-3 induced strong Ab production, and with an enzyme-linked immunosorbent assay, anti-S6-2, anti-S6-4 and anti-S6-5, showed high reactivity to recombinant rpS6 (r-rpS6), while anti-S6-1 did not, suggesting that S6-2, S6-4 and S6-5 were exposed on the r-rpS6 surface, while S6-1 was less exposed or possessed a different conformation. The immunostaining of HeLa cells as well as isolated chromosomes suggested that rpS6 occurs in endoplasmic reticulum (ER) but less possible on chromosomes since no Abs showed localization of rpS6 to chromosomes. In addition, the immunostaining suggested that only S6-4 is exposed on the protein surface, while S6-2 and S6-5 are buried by the interaction with other macromolecules in HeLa cells. Present our result shows new possibility of antibodies as tools for structure-oriented cell biology.

  Mar. 2008, Journal of biochemistry, 143(3) (3), 325 - 32, English, International magazine

  [Refereed]

  Scientific journal


• Interstitial telomere-like repeats in the Haplopappus gracilis (Asteraceae) genome revealed by fluorescence in situ hybridization

  Hidehiro Hanmoto, Ryohei Kataoka, Nobuko Ohmido, Yoshihiko Yonezawa

  Dec. 2007, Cytologia, 72(4) (4), 483 - 488, English

  [Refereed]

  Scientific journal


• Fertile somatic hybrids between Solanum integrifolium and S. sanitwongsei (syn. S. kurzii) as candidates for bacterial wilt-resistant rootstock of eggplant

  Yuzuri Iwamoto, Masashi Hirai, Nobuko Ohmido, Kiichi Fukui, Hiroshi Ezura

  Mar. 2007, PLANT BIOTECHNOLOGY, 24(2) (2), 179 - 184, English

  [Refereed]

  Scientific journal


• Chromosome maps of legumes.

  Nobuko Ohmido, Shusei Sato, Satoshi Tabata, Kiichi Fukui

  Legumes are of immense importance as food and feed, and for sustainable agriculture, due to their ability to fix nitrogen. Here, the chromosome maps of the legumes soybean (Glycine max), Lotus (Lotus japonicus), and red clover (Trifolium pratense) are reviewed. These species have relatively small chromosomes and therefore are difficult to exploit for chromosome studies. Nevertheless, the identification of individual chromosomes became feasible, and chromosome maps have been developed applying image analysis and fluorescence in-situ hybridization. For Lotus japonicus, e.g. detailed chromosome maps have been developed using the information of genetic linkage maps. Future prospects of further legume chromosome mapping for breeding and genetic purposes are discussed.

  2007, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 15(1) (1), 97 - 103, English, International magazine

  [Refereed]

  Scientific journal


• CSCL-based Science Curriculum on the Theme of Social Decision-Making about Genetically Modified Foods : Epistemic Agency in Knowledge Building and Students' Personal Opinions

  SAKAMOTO Miki, INAGAKI Shigenori, YAMAGUCHI Etsuji, FUJIMOTO Masaji, YAMAMOTO Tomokazu, TAKENAKA Makiko, OSHIMA Jun, OSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko

  筆者らは,CSCLシステムであるKnowledge Forumを活用した小学生のための科学教育カリキュラムを開発・評価している.本研究では,遺伝子組換え食品問題に対する社会的意思決定を扱うカリキュラムを,知識構築に関するデザイン原則のひとつである認識主体性の観点から改善し,有効性を評価した.評価の指標は,この問題に対する個人的意見であり,単元開始時,中盤,終了後の3時点で測定し,学習の進行に伴う変化を改善前のカリキュラムでの結果と比較した.その結果,改善後のカリキュラムでは,単元の中盤から提案型の意見が出現し,単元後にはほぼ全員が,条件や代替策を示した賛成意見を記述した.児童の認識主体性を考慮した授業デザインの変更により,共同体にとって価値あるアイディアの創出という知識構築のレベルを,さらに向上させることができた.

  Japan Society for Science Education (JSSE), Aug. 2006, JSSE Research Report, 21(1) (1), 83 - 88, Japanese

  Symposium


• Development of an Empowerment System for Citizens in Science and Technology II : Starting Science Cafe Kobe

  ITOH Masayuki, TANAKA Shigenori, EBINA Kuniyoshi, NAGASAKA Kosaku, OHMIDO Nobuko, OGASAWARA Fumie, SAKURAI Kaori, HAMAOKA Rie

  「市民の科学に対する大学の支援に関する実践的研究」(略称「市民の科学」)プロジェクトの一環として、「サイエンスカフェ神戸」を創始した。このプロジェクトは、科学技術的課題に対する市民のエンパワーメント・システム構築をめざすもので、サイエンスカフェ開催はその第一段階として位置づけられる。2005年10月から2006年6月までに16回を開催し、科学コミュニケーションの新しいスタイルとして高い可能性を確認した。サイエンスカフェは現在各地に広がりつつあるが、「サイエンスカフェ神戸」では、文化としての科学を地域社会に根づかせることを大きな目的とし、運営に市民が主体的に参加し、様々な場で頻繁に開催されるようなあり方をゴールとして設定している点で特徴をもっている。「市民の科学」プロジェクトでは、次のステップとして、サイエンスカフェを通じて形成された緩やかなネットワークも利用しつつ、大学の支援のもとでの、環境などに関わる課題の市民による調査・研究の展開可能性を探ってゆく。

  Japan Society of Science Education, Aug. 2006, 日本科学教育学会研究会研究報告, Vol. 21, No. 1, pp. 37-42(1) (1), 37 - 42, Japanese

  Scientific journal


• Comprehensive Structural Analysis of the Genome of Red Clover (Trifolium pratense L.)

  Tabata Satoshi, Okumura Kenji, Isobe Sachiko, Sato Shusei, Asamizu Erika, Kataoka Ryohei, Ohmido Nobuko, Sakurai Nozomi, Kaneko Takakazu, Nakamura Yasukazu, Klimenko Irina

  With the aim of establishing the basic knowledge and resources needed for applied genetics, we investigated the genome structure of red clover Trifolium pratense L.. The average GC content of the nuclear genome was shown to be 34.2% by analysis of 1.4 Mb of random genomic sequences. A total of 26,356 ESTs, that were grouped into 9339 non-redundant sequences, were collected. A high-density genetic linkage map of the genome was constructed using two parent plants, HR and R130, and188 F1 plants from the mapping population. As a result, a total of 1434 loci detected by 1399 markers (1286 detected by microsatellite markers and 148 by RFLP markers) were successfully mapped onto seven linkage groups totaling 868.7 cM in length. Macrosynteny at the segmental level was observed between the genomes of red clover and two model legumes, Lotus japonicus and Medicago truncatula.

  The Japanese Society of Plant Physiologists, 2006, Plant and Cell Physiology Supplement, 2006(0) (0), 878 - 878


• Chromosome dynamics in tobacco BY-2 cultured cells

  S. Matsunaga, N. Ohmido, K. Fukui

  Springer International Publishing, 2006, Biotechnology in Agriculture and Forestry, 58, 51 - 63, English

  [Refereed]

  Scientific journal


• RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.

  Koichi Sakamoto, Tomoko Abe, Tomoki Matsuyama, Shigeo Yoshida, Nobuko Ohmido, Kiichi Fukui, Shinobu Satoh

  Male-associated DNA sequences were analyzed in Cannabis sativa L. (hemp), a dioecious plant with heteromorphic sex chromosomes. DNA was isolated from male and female plants and subjected to random amplified polymorphic DNA analysis. Of 120 primers, 17 yielded 400 to 1500-bp fragments detectable in male, but not female, plants. These fragments were cloned and used as probes in gel-blot analysis of genomic DNA. When male and female DNA was hybridized with 2 of these male-specific fragments, MADC(male-associated DNA sequences in C. sativa)3 and MADC4, particularly intense bands specific to male plants were detected in addition to bands common to both sexes. The MADC3 and MADC4 sequences were shown to encode gag/pol polyproteins of copia-like retrotransposons. Fluorescence in situ hybridization with MADC3 and MADC4 as probes revealed a number of intense signals on the Y chromosome as well as dispersed signals on all chromosomes. The gel-blot analysis and fluorescence in situ hybridization results presented here support the hypothesis that accumulation of retrotransposable elements on the Y chromosome might be 1 cause of heteromorphism of sex chromosomes.

  Oct. 2005, Genome, 48(5) (5), 931 - 6, English, International magazine

  [Refereed]

  Scientific journal


• Developement of an Empowerment System for Citizens in Science and Technology

  ITOH, Masayuki, OGAWA, Masataka, TAKEDA, Yoshiaki, USHIMARU, Atsushi, TAINOSHO, Yoshiaki, EBINA, Kuniyoshi, OHMIDO, Nobuko, SHIRASUGI, Naoko, NAGASAKA, Kosaku, TANAKA, Shigenori, SANUKIDA, Satoshi, NOBUKAWA, Takako

  科学・技術が高度に発達した社会において、(a)環境問題等の解決手段として、(b)知的探求活動として、市民の科学・技術にかかわる問題の調査・研究能力を高めてゆくこと(エンパワーメント)が大きな意味を持つ。我々は、神戸大学大学院総合人間科学研究科に設置された発達支援インスティテュート/ヒューマン・コミュニティ創成研究センターの研究プロジェクトとして「市民科学に対する大学の支援に関する実践的研究」の取り組みを始めた。本プロジェクトは、神戸を主なフィールドとして、幅広い年齢や素養をもつ市民が、大学の支援のもとに、科学リテラシーを高めるとともに、自らが調査・研究能力を獲得してゆく持続可能なシステムとそれを担う組織、人材のあり方を実践的に探り、日本の社会に適したモデルを構築することを目指す。

  Japan Society of Science Education, Sep. 2005, 科教研報, 20・2, 47-51(2) (2), 47 - 51, Japanese

  Scientific journal


• CSCL-based Science Curriculum on the theme of Social Decision-Making about Genetic Modified Foods: How Pupils Change Their Personal Opinions through the Curriculum?

  SAKAMOTO, Miki, FUJIMOTO, Masaji, INAGAKI, Shigenori, YAMAGUCHI, Etsuji, TAKENAKA, Makiko, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, OHMIDO, Nobuko, TACHIBANA, Sanae, YAMAMOTO, Tomokazu

  筆者らは, CSCLシステムであるKnowledge Forumを小学校へ導入し, 遺伝子組換え食品問題に対する社会的意思決定をテーマとした, 小学生のための科学教育カリキュラムを開発・改善している.本研究では, 改善したカリキュラムの有効性を評価する一環として, 単元開始時, 中盤, 終了後の3時点で, 遺伝子組換え食品問題に対する個人的意見を記述させ, 学習の進行に伴う変化を検討した.その結果, 学習の進行に伴って賛否の立場の分布が変容したことに加え, その理由の記述が, イメージによるものから, エビデンスに基づくものや提案型のものへと変化したことが明らかになった.カリキュラムの目標である社会的意思決定の能力を獲得したことにより, 学習者は, 論争性の把握や対立の解消が明示的に要求されない場面においても, 賛否両論のエビデンスやそのバランスを考慮して, 建設的な意見を表明できるようになったといえる.

  Japan Society of Science Education, Sep. 2005, 日本科学教育学会研究会研究報告, 20・2, p1-p6(2) (2), 1 - 6, Japanese

  Scientific journal


• CSCL-based Science Curriculum on the Theme of Social Decision-Makingabout Genetic Modification Foods: Conceptual Understanding and Change of Image of Elementary School Child Who Studied about the 2004 Version of the Curriculum

  FUJIMOTO, Masaji, SAKAMOTO, Miki, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, OHMIDO, Nobuko, TACHIBANA, Sanae, YAMAMOTO, Tomokazu

  筆者らは, CSCLシステムのKnowledge Forumを活用し, 遺伝子組換え食品問題に対する社会的意思決定をテーマとした科学教育カリキュラムを開発・改善している.2004年度版カリキュラムは, 2003年度版カリキュラムの評価結果で指摘された問題点を改善したものであった.本研究では, 改善したカリキュラム評価の一環として, 概念的理解とイメージの変容に関する評価結果を年度間で比較した.その結果, 遺伝子組換え食品に関する社会的な論争性の理解を深めるとともに, 遺伝子組換え食品に対するネガティブなイメージをポジティブとネガティブが混在するイメージへ変容させる点において, 2004年度版カリキュラムの高い効果が認められた.

  Japan Society of Science Education, Aug. 2005, 日本科学教育学会研究会研究報告, 20・1, p15-p20(1) (1), 15 - 20, Japanese

  Scientific journal


• Comparison of surface structures between extended and condensed stages of barley chromosomes revealed with atomic force microscopy

  Nobuko Ohmido, Kyoko Kijima, Osamu Hoshi, Tatsuo Ushiki, Kiichi Fukui

  Mar. 2005, Cytologia, 70(1) (1), 101 - 108, English

  [Refereed]

  Scientific journal


• CSCL-based Science Curriculum on the Theme of Social Decision-Making about Genetic Modification Foods : Achievement of Social Decision-Making of Elementary School Child Who Studied about the 2004 Version of the Curriculum

  FUJIMOTO Masaji, SAKAMOTO Miki, INAGAKI Shigenori, TAKENAKA Makiko, YAMAGUCHI Etsuji, OSHIMA Jun, OHSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko, TACHIBANA Sanae, YAMAMOTO Tomokazu

  筆者らは,CSCLシステムのKnowledge Forumを小学校へ導入し,遺伝子組換え食品問題に対する社会的意思決定をテーマとした小学生のための科学教育カリキュラムを開発・改善している.2004年度は,基礎的内容に関する理解促進および社会的意思決定の洗練という観点から,2003年度版カリキュラムを改善している.実験授業を実施し,学習者の社会的意思決定を適切性の観点から評価したところ,2004年度の達成度が2003年度よりも向上したことがわかった.

  Japan Society for Science Education, 2005, Proceedings of the Annual Meeting of Japan Society for Science Education, 29(0) (0), 527 - 528, Japanese

  Scientific journal


• CSCL-based Science Curriculum on the Theme of Social Decision-Making about Genetic Modification Foods : Can Pupils Transfer their Decision-Making to Other Issues?

  SAKAMOTO Miki, FUJIMOTO Masaji, INAGAKI Shigenori, YAMAGUCHI Etsuji, TAKENAKA Makiko, OSHIMA Jun, OHSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko, TACHIBANA Sanae, YAMAMOTO Tomokazu

  筆者らは,遺伝子組換え食品問題に対する社会的意思決定をテーマとした小学生のための科学教育カリキュラムを開発している.本研究では,このカリキュラムの評価の一環として,育成した社会的意思決定の力,すなわち賛否両論を踏まえ,そのバランスを考慮しながらコンセンサスを創出する能力が,他の科学技術問題に転移するかどうか検討した.カリキュラムの前後で,原子力発電に関する社会的意思決定を学習者に課し,記述の内容を比較した結果,転移が認められ,特にコンセンサスの観点で向上が見られた.

  Japan Society for Science Education, 2005, Proceedings of the Annual Meeting of Japan Society for Science Education, 29(0) (0), 525 - 526, Japanese

  Scientific journal


• Comprehensive structural analysis of the genome of red clover (Trifolium pratense L.).

  Shusei Sato, Sachiko Isobe, Erika Asamizu, Nobuko Ohmido, Ryohei Kataoka, Yasukazu Nakamura, Takakazu Kaneko, Nozomi Sakurai, Kenji Okumura, Irina Klimenko, Shigemi Sasamoto, Tsuyuko Wada, Akiko Watanabe, Mitsuyo Kohara, Tsunakazu Fujishiro, Satoshi Tabata

  With the aim of establishing the basic knowledge and resources needed for applied genetics, we investigated the genome structure of red clover Trifolium pratense L. by a combination of cytological, genomic and genetic approaches. The deduced genome size was approximately 440 Mb, as estimated by measuring the nuclear DNA content by flow cytometry. Seven chromosomes could be distinguished by microscopic observation of DAPI stained prometaphase chromosomes and fluorescence in situ hybridization using 28S and 5S rDNA probes and bacterial artificial chromosome probes containing microsatellite markers with known positions on a genetic linkage map. The average GC content of the genomes of chloroplast, mitochondrion and nucleus were shown to be 33.8, 42.9 and 34.2%, respectively, by the analysis of 1.4 Mb of random genomic sequences. A total of 26,356 expressed sequence tags (ESTs) that were grouped into 9339 non-redundant sequences were collected, and 78% of the ESTs showed sequence similarity to registered genes, mainly of Arabidopsis thaliana and rice. To facilitate basic and applied genetics in red clover, we generated a high-density genetic linkage map with gene-associated microsatellite markers. A total of 7159 primer pairs were designed to amplify simple sequence repeats (SSRs) identified in four different types of libraries. Based on sequence similarity, 82% of the SSRs were likely to be associated with genes. Polymorphism was examined using two parent plants, HR and R130, and 10 F(1) progeny by agarose gel electrophoresis, followed by genotyping for the primer pairs showing polymorphisms using 188 F(1) plants from the mapping population. The selected 1305 microsatellite markers as well as the previously developed 167 restriction fragment length polymorphism markers were subjected to linkage analysis. A total of 1434 loci detected by 1399 markers were successfully mapped onto seven linkage groups totaling 868.7 cM in length; 405 loci (28%) were bi-parental, 611 (43%) were specific to HR and 418 (29%) were specific to R130. Each genetic linkage group was linked to a corresponding chromosome by FISH analysis using seven microsatellite markers specific to each of the linkage groups as probes. Transferability of the developed microsatellite markers to other germplasms was confirmed by testing 268 selected markers on 88 red clover germplasms. Macrosynteny at the segmental level was observed between the genomes of red clover and two model legumes, Lotus japonicus and Medicago truncatula, strongly suggesting that the genome information for the model legumes is transferable to red clover for genetic investigations and experimental breeding.

  2005, DNA research : an international journal for rapid publication of reports on genes and genomes, 12(5) (5), 301 - 64, English, International magazine

  [Refereed]

  Scientific journal


• CSCL-based Science Curriculum on the theme of Social Decision-Making about Genetic Modification Foods: The 2004 Version of the Curriculum and Analysis of Students' Understanding of Basic Contents

  FUJIMOTO, Masaji, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, SAKAMOTO, Miki, OHMIDO, Nobuko, YAMAMOTO, Tomokazu, TACHIBANA, Sanae, TAKESHITA, Yuko

  筆者らは, CSCLシステムのKnowledge Forumを導入し, 遺伝子組換え食品問題に対する社会的意思決定をテーマとした小学生のための科学教育カリキュラムを開発している.2004年度は, 基礎的内容に関する理解の促進, および社会的意思決定の洗練という観点から, 昨年度のカリキュラムを改善した.基礎的内容に関する理解度を評価した結果, 昨年よりも理解度が向上したことが明らかになった.

  Japan Society of Science Education, Jan. 2005, 日本科学教育学会研究会研究報告, 19・3, p33-p38(3) (3), 33 - 38, Japanese

  Scientific journal


• Fluorescent labeling of plant chromosomes in suspension by FISH.

  Ohmido N

  Jan. 2005, Genes Genet. Syst., 2: 35-39

  [Refereed]

  Scientific journal


• Chromosome painting as a tool for rice genetics and breeding.

  Shishido, R, Ohmido, N, Fukui, K

  Nov. 2004, Methods in Cell Science, English


• CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetically Modified Foods: Evaluation of Students' Achievement on the Learning Unit

  SAKAMOTO, Miki, TAKENAKA, Makiko, INAGAKI, Shigenori, YAMAGUCHI, Etsuji, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, YAMAMOTO, Tomokazu, FUJIMOTO, Masaji, OHMIDO, Nobuko, TAKESHITA, Yuko

  Oct. 2004, 日本科学教育学会研究会研究報告, 19・1, p39-p44, 427 - 428, Japanese

  Scientific journal


• Design Experiments in Elementary Science Lessond Using CSCL Systems

  INAGAKI, Shigenori, YAMAMOTO, Tomokazu, KURODA, Hideko, TACHIBANA, Sanae, TAKESHITA, Yuko, FUJIMOTO, Masaji, OSHIMA, Jun, NAKAYAMA, Hayashi, YAMAGUCHI, Etsuji, MURAYAMA, Isao, TAKENAKA, Makiko, OSHIMA, Ritsuko, FUNAOI, Hideo, DEGUCHI, Akiko, SUZUKI, Hideyuki, KATO, Hiroshi, OHKUBO, Masahiko, TAKEDA, Yoshiaki, TAINOSHO, Yoshiaki, KOISHI, Hirofumi, DOI, Shozo, ITOH, Masako, SAKAMOTO, Miki, HATONO, Itsuo, IKARI, Miwa, MOCHIZUKI, Toshio, OGAWA, Masakato, OHMIDO, Nobuko

  本研究では,神戸大学発達科学部附属住吉小学校をフィールドとして展開されているCSCLシステムを利用した授業のデザイン実験の実際について,Knowledge Forum,再構成型コンセプトマップ作成ソフトウェア「あんどう君」,ケータイ利用の野外情報共有システム(clippic)の事例を公開授業として報告する.

  Japan Society of Science Education, Oct. 2004, 日本科学教育学会研究会研究報告, 19・1, p1-p4(1) (1), 1 - 4, Japanese

  Scientific journal


• 6I7-13 CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetic Modification Foods : Analyses of Achievement of Social Decision-Making

  FUJIMOTO Masaji, INAGAKI Shigenori, TAKENAKA Makiko, YAMAGUCHI Etsuji, YAMAMOTO Tomokazu, SAKAMOTO Miki, OSHIMA Jun, OSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko, TAKESHITA Yuko

  Japan Society for Science Education (JSSE), Jul. 2004, 年会論文集, 28, 427 - 428, Japanese

  Scientific journal


• 6I7-12 CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetically Modified Foods : How Students Change Their Conceptual Understanding and Image

  SAKAMOTO Miki, INAGAKI Shigenori, TAKENAKA Makiko, YAMAGUCHI Etsuji, FUJIMOTO Masaji, YAMAMOTO Tomokazu, OSHIMA Jun, OSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko, AKESHITA Yuko

  Japan Society for Science Education (JSSE), Jul. 2004, 年会論文集, 28, 425 - 426, Japanese

  Scientific journal


• 6I7-11 CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetic Modification Foods : Analysis of Students' Understanding of Basic Contents

  YAMAMOTO Tomokazu, INAGAKI Shigenori, TAKENAKA Makiko, YAMAGUCHI Etsuji, FUJIMOTO Masaji, SAKAMOTO Miki, OSHIMA Jun, OSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko, TAKESHITA Yuko

  Japan Society for Science Education (JSSE), Jul. 2004, 年会論文集, 28, 423 - 424, Japanese

  Scientific journal


• Application of the bio-active beads method in rice transformation

  Haibo Liu, Akira Kawabe, Sachihiro Matsunaga, Akio Kobayashi, Satoshi Harashima, Susumu Uchiyama, Nobuko Ohmido, Kiichi Fukui

  Japanese Society for Plant Cell and Molecular Biology, 2004, Plant Biotechnology, 21(4) (4), 303 - 306, English

  [Refereed]

  Scientific journal


• Chromosomal rearrangements in interspecific hybrids between Nicotiana gossei Domin and N-tabacum L., obtained by crossing with pollen exposed to helium ion beams or gamma-rays

  S Kitamura, M Inoue, N Ohmido, K Fukui, A Tanaka

  May 2003, NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 206, 548 - 552, English

  [Refereed]

  Scientific journal


• Development of a quantitative pachytene chromosome map in Oryza sativa by imaging methods

  S Kato, N Ohmido, M Fukui

  Apr. 2003, GENES & GENETIC SYSTEMS, 78(2) (2), 155 - 161, English

  [Refereed]

  Scientific journal


• High resolution FISH for gene mapping and molecular analysis of rice chromosomes.

  Ohmido, N, Fukui, K

  Chinese Academic Publishers, Beijin, Mar. 2002, Advances in Chromosome Sciences, English


• Trends in site-number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae)

  Misako Mishima, Nobuko Ohmido, Kiichi Fukui, Tetsukazu Yahara

  Springer Verlag, 2002, Chromosoma, 110(8) (8), 550 - 558, English

  [Refereed]

  Scientific journal


• Rapid DNA fiber technique for size measurements of linear and circular DNA probes

  O Henegariu, L Grober, W Haskins, PN Bowers, MW State, N Ohmido, P Bray-Ward, DC Ward

  Aug. 2001, BIOTECHNIQUES, 31(2) (2), 246 - +, English

  [Refereed]

  Scientific journal


• A new gypsy-type retrotransposon, RIRE7: preferential insertion into the tandem repeat sequence TrsD in pericentromeric heterochromatin regions of rice chromosomes

  N Kumekawa, N Ohmido, K Fukui, E Ohtsubo, H Ohtsubo

  May 2001, MOLECULAR GENETICS AND GENOMICS, 265(3) (3), 480 - 488, English

  [Refereed]

  Scientific journal


• Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISH

  R Nakamura, S Kitamura, M Inoue, N Ohmido, K Fukui

  May 2001, THEORETICAL AND APPLIED GENETICS, 102(6-7) (6-7), 810 - 814, English

  [Refereed]

  Scientific journal


• High resolution physical mapping of genes of plant chromosomes

  OHMIDO

  May 2001, Chromosome Sci., 5, 174p-179p, English

  Scientific journal


• Visualization of specific DNA sequences on chromosomes and extended DNA fibers.

  Cartagena, J. A, Sykorova, E, Fajkus, J, Haibo, L, Ohmido, N, Ito, M, Fukui, K

  Feb. 2001, Ann.Rep.IC Biotech., English


• A new gypsy-type retrotransposon, RIRE7: Preferential insertion into the tandem repeat sequence TrsD in pericentromeric heterochromatin regions of rice chromosomes

  N. Kumekawa, N. Ohmido, K. Fukui, E. Ohtsubo, H. Ohtsubo

  2001, Molecular and General Genetics, 265(3) (3), 480 - 488, English

  [Refereed]

  Scientific journal


• Chromosome painting as a tool for rice genetics and breeding

  Rieko Shishido, Nobuko Ohmido, Kiichi Fukui

  2001, Methods in Cell Science, 23(1-3) (1-3), 125 - 132, English

  Scientific journal


• Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers

  Nobuko Ohmido, Kyoko Kijima, Ikuo Ashikawa, J. Hans De Jong, Kiichi Fukui

  2001, Plant Molecular Biology, 47(3) (3), 413 - 421, English

  [Refereed]

  Scientific journal


• Estimation of the nuclear DNA content of strawberries (Fragaria spp.) Compared with Arabidopsis thaliana by using dual-step flow cytometry

  Yukio Akiyama, Yosnihisa Yamamoto, Nobuko Ohmido, Masahiro Ohshima, Kiichi Fukui

  Japan Mendel Society, 2001, Cytologia, 66(4) (4), 431 - 436, English

  [Refereed]

  Scientific journal


• Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa

  K Sakamoto, N Ohmido, K Fukui, H Kamada, S Satoh

  Dec. 2000, PLANT MOLECULAR BIOLOGY, 44(6) (6), 723 - 732, English

  [Refereed]

  Scientific journal


• Quantitative chromosome maps and rDNA localization in the T subgenome of Nicotiana tabacum L. and its putative progenitors

  S Kitamura, M Inoue, N Ohmido, K Fukui

  Dec. 2000, THEORETICAL AND APPLIED GENETICS, 101(8) (8), 1180 - 1188, English

  [Refereed]

  Scientific journal


• Visual detection of useful genes on plant chromosomes.

  Fukui, K, Ohmido, N

  Nov. 2000, Jpn. Agr. Res.Quart., English


• Quantification of total genomic DNA and selected repetitive sequences reveals concurrent changes in different DNA families in indica and japonica rice

  N Ohmido, K Kijima, Y Akiyama, JH de Jong, K Fukui

  Apr. 2000, MOLECULAR AND GENERAL GENETICS, 263(3) (3), 388 - 394, English

  [Refereed]

  Scientific journal


• Quantitative chromosome map of Arabidopsis thaliana L. by imaging methods

  ITO, OHMIDO, AKIYAMA, FUKUI

  Mar. 2000, Cytologia, 65, 325p-331p, English

  [Refereed]

  Scientific journal


• Smallness: gain and loss in plant chromosome research

  K Fukui, N Ohmido, T Wako

  2000, CHROMOSOMES TODAY, VOL 13, 13, 287 - +, English

  [Refereed]

  International conference proceedings


• Characterization of spinach chromosomes by condensation patterns and physical mapping of 5S and 45S rDNAs by FISH

  M Ito, N Ohmido, Y Akiyama, K Fukui, T Koba

  Jan. 2000, JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 125(1) (1), 59 - 62, English

  [Refereed]

  Scientific journal


• Rice genome research: An alternative approach based on molecular cytology

  K Fukui, N Ohmido

  2000, GENOMES, , 109p-121p, 109 - 121, English

  [Refereed]

  International conference proceedings


• Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants

  T Sone, M Fujisawa, M Takenaka, S Nakagawa, S Yamaoka, M Sakaida, R Nishiyama, KT Yamato, N Ohmido, K Fukui, H Fukuzawa, K Ohyama

  Nov. 1999, PLANT MOLECULAR BIOLOGY, 41(5) (5), 679 - 685, English

  [Refereed]

  Scientific journal


• Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods

  S Ha, PH Moore, D Heinz, S Kato, N Ohmido, K Fukui

  Apr. 1999, PLANT MOLECULAR BIOLOGY, 39(6) (6), 1165 - 1173, English

  [Refereed]

  Scientific journal


• Recent advances in the physical mapping of genes by fluorescence in situ hybridization (FISH) of rice

  Nobuko Ohmido, Kyoko Kijima, Tamaki Hirose, Hans De Jong, Kiichi Fukui

  Feb. 1999, American Biotechnology Laboratory, 17(3) (3), 56 - 58, English

  Scientific journal


• Erratum: Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods (Plant Molecules Biology 39 (1165-1173))

  S. Ha, P. H. Moore, D. Heinz, S. Kato, N. Ohmido, K. Fukui

  Springer Netherlands, 1999, Plant Molecular Biology, 41(2) (2), 293, English

  Scientific journal


• Physical mapping of 18S rDNA by fluorescence in situ hybridization (FISH) in the three species of the genus Paris L., Liliaceae

  Junko Miyamoto, Nobuko Ohmido, Kiichi Fukui

  Japan Mendel Society, 1999, Cytologia, 64(2) (2), 175 - 180, English

  [Refereed]

  Scientific journal


• Physical mapping of unique nucleotide sequences on identified rice chromosomes

  N Ohmido, Y Akiyama, K Fukui

  Dec. 1998, PLANT MOLECULAR BIOLOGY, 38(6) (6), 1043 - 1052, English

  [Refereed]

  Scientific journal


• Characterization of parental genomes in a hybrid between Oryza sativa L. and O. officinalis Wall ex Watt. through fluorescence in situ hybridization.

  ASGHAR, BRAR, HERNANDEZ, OHMIDO, KHUSH

  Dec. 1998, Rice Genetics News, 15, 83p-84p, English

  Scientific journal


• Isolation and FISH mapping of Yeast Artificial Chromosomes (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in rice

  KR Rajyashri, S Nair, N Ohmido, K Fukui, N Kurata, T Sasaki, M Mohan

  Sep. 1998, THEORETICAL AND APPLIED GENETICS, 97(4) (4), 507 - 514, English

  [Refereed]

  Scientific journal


• RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides

  A Escalante, S Imanishi, M Hossain, N Ohmido, K Fukui

  May 1998, THEORETICAL AND APPLIED GENETICS, 96(6-7) (6-7), 719 - 726, English

  [Refereed]

  Scientific journal


• Agronomic and Cytological Characteristics of F1 Hybrids between Lolium multiflorum Lam. and Festuca arundinacea var. glaucescens Boiss.

  MIZUKAMI, SUGITA, OHMIDO, FUKUI

  In order to evaluate the agronomic importance of amphitetraploid F 1 hybrids between Lolium multiflorum Lam. (2n=4x=28) and Festuca arundinacea var. glaucescens Boiss. (2n=4x=28), several agronomic and cytological characters were examined. The general appearance of F 1 hybrids produced using an embryo-culture was similar to L. multiflorum. Their agronomic characters, including plant height, first heading date and regrowth vigor, tended toward the middle of the parental characters or were rather more similar to the tetraploid L. multiflorum (cv. Aki-aoba). The total fresh weight per year of F 1 hybrids was equal to the tetraploid L. multiflorum and F. arundinacea (2n=6x=42, cvs. Lubrette, Hokuryo). The dry matter digestibility was equal or superior to that of F. arundinacea. The pollen fertility and seed yield were sufficient for ordinary propagation. These results suggest the possibility of producing a new Festulolium line with good agronomic performance from the tetraploid hybrids obtained in this study. Though there was no aneuploid in F 1 hybrids, some multivalents and/or univalents were observed. Parental chromosomes were identified in the F 1 hybrids using genomic in situ hybridization (GISH). GISH was found to be useful in detecting the changes of parental genomes among the progenies in the breeding programs.

  Japanese Society of Grassland Science, Apr. 1998, Grassland Science, 44-1, 14p-21p(1) (1), 14 - 21, English

  [Refereed]

  Scientific journal


• Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomes

  K Fukui, S Nakayama, N Ohmido, H Yoshiaki, M Yamabe

  Mar. 1998, THEORETICAL AND APPLIED GENETICS, 96(3-4) (3-4), 325 - 330, English

  [Refereed]

  Scientific journal


• Multi-dimensional analyses of plant chromosomes and genomes.

  FUKUI, OHMIDO, WAKO

  1998, The 12th Symposium on Plant Biotech, 12, 61p-70p, English

  International conference proceedings


• Detection of specific chromosome reduction in rice somatic hybrids with the A, B, and C genomes by multi-color genomic in situ hybridization

  R. Shishido, S. Apisitwanich, N. Ohmido, Y. Okinaka, K. Mori, K. Fukui

  1998, Theoretical and Applied Genetics, 97(7) (7), 1013 - 1018, English

  [Refereed]

  Scientific journal


• Visual verification of close disposition between a rice A genome-specific DNA sequence (TrsA) and the telomere sequence

  N Ohmido, K Fukui

  Dec. 1997, PLANT MOLECULAR BIOLOGY, 35(6) (6), 963 - 968, English

  [Refereed]

  Scientific journal


• Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza

  S Uozu, H Ikehashi, N Ohmido, H Ohtsubo, E Ohtsubo, K Fukui

  Dec. 1997, PLANT MOLECULAR BIOLOGY, 35(6) (6), 791 - 799, English

  [Refereed]

  Scientific journal


• Chromosomal location and reorganization of the 45S and 5S rDNA in the Brachyscome lineariloba complex (Asteraceae)

  J Adachi, K Watanabe, K Fukui, N Ohmido, K Kosuge

  Sep. 1997, JOURNAL OF PLANT RESEARCH, 110(1099) (1099), 371 - 377, English

  [Refereed]

  Scientific journal


• Analysis of the Chromosome Composition of Common Wheat-Agropyron intermedium Partial Amphiploid,Yuan Zhong 2 by in situ hybridization.

  MA, TOMITA, NAKATA, YASUMURO, OHMIDO, FUKUI

  Jun. 1997, Chinese Journal of Genetics, 24-3, 199p-223p, English

  [Refereed]

  Scientific journal


• Construction of an 800-kb contig in the near-centromeric region of the rice blast resistance gene Pi-ta(2) using a highly representative rice BAC library

  S Nakamura, S Asakawa, N Ohmido, K Fukui, N Shimizu, S Kawasaki

  May 1997, MOLECULAR & GENERAL GENETICS, 254(6) (6), 611 - 620, English

  [Refereed]

  Scientific journal


• Identification of parental chromosomes in the interspecific hybrids of Nicotiana rustica L x N-tabacum L and N-gossei Domin x N-tabacum L, using genomic in situ hybridization

  S Kitamura, M Inoue, N Ohmido, K Fukui

  Mar. 1997, BREEDING SCIENCE, 47(1) (1), 67 - 70, English

  [Refereed]

  Scientific journal


• Identification of parental chromosomes in the interspecific hybrids of Nicotiana rustica L. x N. tabacum L. and N. gossei Domin x N. tabacum L., using genomic in situ hybridization

  Satoshi Kitamura, Masayoshi Inoue, Nobuko Ohmido, Kiichi Fukui

  Japanese Society of Breeding, 1997, Breeding Science, 47(1) (1), 67 - 70, English

  [Refereed]

  Scientific journal


• The distribution of 5-methylcytosine in somatic chromosomes of germinating aged rice seeds

  FIUMINHAN, OHMIDO, FUKUI, MIZUGAKI, KAMEYA

  Dec. 1996, Maize Genet. Coop. Newslett, 71, 162p-166p, English

  Scientific journal


• Heterochromatic knob-specific repeated sequence is associated with the formation of chromosome bridges in cultured cells and in germinating roots of aged seeds.

  FIUMINHAN, OHMIDO, FUKUI, KAMEYA

  Mar. 1996, Maize Genet. Coop. Newslett, 70, 60p-61p, English

  Scientific journal


• Cytological studies of African cultivated rice, Oryza glaberrima Steud. Theor. Appl.

  Ohmido, N, K. Fukui

  Jun. 1995, Theor. Appl. Genet., 91, 212 - 217, English

  Scientific journal


• Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridization

  K. Fukui, N. Ohmido, G. S. Khush

  1994, Theoretical and Applied Genetics: International Journal of Plant Breeding Research, 87(8) (8), 893 - 899, English

  Scientific journal


• MICRODISSECTION OF PLANT CHROMOSOMES BY ARGON-ION LASER-BEAM

  K FUKUI, M MINEZAWA, Y KAMISUGI, M ISHIKAWA, N OHMIDO, T YANAGISAWA, M FUJISHITA, F SAKAI

  Sep. 1992, THEORETICAL AND APPLIED GENETICS, 84(7-8) (7-8), 787 - 791, English

  [Refereed]

  Scientific journal

• 科学技術の社会問題としてのゲノム編集を題材とした小学生向け教育プログラムの評価： 複数のステイクホルダーの意見に関する学習を支援するダイヤローグ型ビデオ教材に着目して

  口羽駿平, 山口悦司, 坂本美紀, 山本智一, 原愛佳, 近江戸伸子, 俣野源晃, 澁野哲

  May 2023, 日本科学教育学会研究会研究報告, 37(6) (6), 59 - 62, Japanese


• 科学技術の社会問題としてのゲノム編集を題材とした小学生向け教育プログラムの開発

  口羽 駿平, 山口 悦司, 坂本 美紀, 山本 智一, 原 愛佳, 近江戸 伸子, 俣野 源晃, 澁野 哲

  Feb. 2023, 日本科学教育学会研究会研究報告, 37(5) (5), 75 - 78, Japanese


• ホウレンソウの雄決定遺伝子座周辺から見出された組換抑制領域の分子構造

  小野寺康之, 工藤友裕, 高橋光彦, 長部高之, 豊田敦, 平川英樹, 鈴木穣, 近江戸伸子

  2018, 園芸学研究 別冊, 17(1) (1)


• 2La12 Phylogenetic analysis of Ty1-copia retrotransposons in Jatropha curcas L. :

  ALIPOUR Atefeh, Cartagena Joyce A, Tsuchimoto Suguru, Ohmido Nobuko, Uchiyama Susumu, Fukui Kiichi

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 197 - 197, English


• P15 アーギュメンテーション・スキルの育成を目指した実践的研究 : 小学校第6学年の理科授業デザイン(研究発表(ポスター発表))

  村津 啓太, 山本 智一, 稲垣 成哲, 近江戸 伸子, 坂本 美紀, 山口 悦司, 神山 真一

  日本理科教育学会近畿支部大会実行委員会, 2011, 日本理科教育学会近畿支部大会(大阪大会)発表要旨集, 2011, 61 - 61, Japanese


• Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization (FISH).

  Nobuko Ohmido, Kiichi Fukui, Toshiro Kinoshita

  Fluorescence in situ hybridization (FISH) is an effective method for the physical mapping of genes and repetitive DNA sequences on chromosomes. Physical mapping of unique nucleotide sequences on specific rice chromosome regions was performed using a combination of chromosome identification and highly sensitive FISH. Increases in the detection sensitivity of smaller DNA sequences and improvements in spatial resolution have ushered in a new phase in FISH technology. Thus, it is now possible to perform in situ hybridization on somatic chromosomes, pachytene chromosomes, and even on extended DNA fibers (EDFs). Pachytene-FISH allows the integration of genetic linkage maps and quantitative chromosome maps. Visualization methods using FISH can reveal the spatial organization of the centromere, heterochromatin/euchromatin, and the terminal structures of rice chromosomes. Furthermore, EDF-FISH and the DNA combing technique can resolve a spatial distance of 1 kb between adjacent DNA sequences, and the detection of even a 300-bp target is now feasible. The copy numbers of various repetitive sequences and the sizes of various DNA molecules were quantitatively measured using the molecular combing technique. This review describes the significance of these advances in molecular cytology in rice and discusses future applications in plant studies using visualization techniques.

  2010, Proceedings of the Japan Academy. Series B, Physical and biological sciences, 86(2) (2), 103 - 16, English, Domestic magazine

  [Refereed]


• テクノ・トレンド FISH法の新しい展開とそれを用いた高精細ゲノム解析法

  近江戸 伸子, 福井 希一

  羊土社, Jul. 2007, バイオテクノロジージャーナル, 7(4) (4), 460 - 463, Japanese


• P-04. Establishment of red clover chromosome map by the improved chromosome image analyzing system, CHIAS4(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :

  HARA Masaki, KATAOKA Ryohei, KATO Seiji, ISOBE Sachiko, SATO Shusei, TABATA Satoshi, OHMIDO Nobuko

  Society of Chromosome Research, 2007, Chromosome science, 10(4) (4), 124 - 124, English


• O-16. The characteristic condensation patterns in rice chromosome(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :

  OHMIDO Nobuko, IWATA Aiko, KATO Seiji, FUKUI Kiichi

  Society of Chromosome Research, 2007, Chromosome science, 10(4) (4), 115 - 115, English


• O-15. Construction of red clover (Trifolium pratense L.) chromosome map(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :

  KATAOKA Ryohei, HARA Masaki, KATO Seiji, ISOBE Sachiko, SATO Shusei, TABATA Satoshi, OHMIDO Nobuko

  Society of Chromosome Research, 2007, Chromosome science, 10(4) (4), 115 - 115, English


• Legume chromosome studies of Trifolium pratense and Lotus japonicus

  Nobuko Ohmido, Seiji Kato, Ryohei Kataoka, Alciko Ishimaru, Sachiko Isobe, Shusei Sato, Satoshi Tabata

  2007, CHROMOSOME RESEARCH, 15, 56 - 56, English

  Summary international conference


• Quantitative and fine rice pachytene chromosome map by analyzed chromomeric features

  Aiko Iwata, Seiji Kato, Kiichi Fukui, Nobuko Ohmido

  2007, CHROMOSOME RESEARCH, 15, 55 - 56, English

  Summary international conference


• O-32. Integration of linkage and chromosome maps of red clover(Trifolium pratense L.)(Abstracts of the oral and poster presentations) :

  KATAOKA Ryohei, KATO Seiji, ISOBE Sachiko, SATO Shusei, TABATA Satoshi, OHMIDO Nobuko

  Society of Chromosome Research, 2006, Chromosome science, 9(4) (4), 127 - 127, English


• Advances in rice chromosomes research.

  Ohmido, N

  Rice chromosome research was initiated around 1910 and has been progressing ever since. Twelve pairs of chromosomes (2n = 24) and a 440 Mb genome, the smallest genome among all main cereal crops, have been identified. According to the recent rice chromosome researches, all chromosomes were well identified over the disadvantages of small chromosome size and quantitative rice chromosomal maps have been developed using chromosome image analysis system (CHIAS). Moreover, agriculturally important genes have been detected using new direct DNA visualization methods with fluorescence in situ hybridization (FISH). This review describes the recent progress in molecular and digital cytogenetics using visualization from a historical perspective, highlighting the future prospects in rice chromosome science.
  
  
  (Communicated by Eishiro SHIKATA, M.J.A.)
  

  The Japan Academy, Oct. 2005, The Proceedings of the Japan Academy, Series B., 12: 382-392.(9) (9), 382 - 392, English

  [Refereed]

  Introduction scientific journal


• Advances in rice chromosomes research.

  N. Ohmido, K. Fukui, T. Kinoshita

  Rice chromosome research was initiated around 1910 and has been progressing ever since. Twelve pairs of chromosomes (2n = 24) and a 440 Mb genome, the smallest genome among all main cereal crops, have been identified. According to the recent rice chromosome researches, all chromosomes were well identified over the disadvantages of small chromosome size and quantitative rice chromosomal maps have been developed using chromosome image analysis system (CHIAS). Moreover, agriculturally important genes have been detected using new direct DNA visualization methods with fluorescence in situ hybridization (FISH). This review describes the recent progress in molecular and digital cytogenetics using visualization from a historical perspective, highlighting the future prospects in rice chromosome science.
  
  
  (Communicated by Eishiro SHIKATA, M.J.A.)
  

  The Japan Academy, Apr. 2005, The Proceedings of the Japan Academy, Series B12., vol. 81, pp.382-392(9) (9), 382 - 392, English

  [Refereed]

  Introduction scientific journal


• アカクローバゲノムの構造解析

  田畑哲之, 佐藤修正, 浅水理恵香, 片平遼平, 近江戸伸子, 笹本茂美, 桜井望, 金子貴一, 中村保一, 渡辺安希子, KLIMENKO Irina, 奥村健治, 磯部祥子

  2005, 日本分子生物学会年会講演要旨集, 28th


• キーワード 人間と発達 V. 生活とテクノロジー

  OHMIDO NOBUKO

  2005, 大学教育出版, Japanese

  Introduction research institution


• ゲノミクス解析システム ゲノム構造解析システム (バイオ高性能機器・新技術利用マニュアル) -- (生体高分子の包括的解析のためのシステム)

  佐藤 修正, 近江戸 伸子, 中村 保一

  共立出版, Aug. 2004, 蛋白質核酸酵素, 49(11) (11), 1828 - 1833,1459, Japanese


• Strategies for structural anaysis of genomes

  Ohmido N

  Aug. 2004, Tanpakushitsu Kakusan Koso., No. 8 -11, pp. 1828-1833, Japanese

  Introduction scientific journal


• P-15 Knowledge Forumを利用した学習環境のデザイン実験 : 遺伝子組換え食品を題材にした単元の開発III

  藤本 雅司, 稲垣 成哲, 竹中 真希子, 山口 悦司, 大島 純, 大島 律子, 村山 功, 中山 迅, 坂本 美紀, 近江戸 伸子, 山本 智一, 橘 早苗, 竹下裕子

  日本理科教育学会近畿支部大会実行委員会, 2004, 日本理科教育学会近畿支部大会(大阪大会)発表要旨集, 2004, 72 - 72, Japanese


• 1pC16-2 Localization of metaphase chromosome-related proteins with indirect immunofluorescent staining

  HAYASHIHARA Kayoko, UCHIYAMA Susumu, KOBAYASHI Shouhei, OHMIDO Nobuko, MATSUNAGA Sachihiro, FUKUI Kiichi

  日本生物工学会, 2004, 日本生物工学会大会講演要旨集, 16, 68 - 68, Japanese


• アカクローバ高密度連鎖地図の作製

  田畑哲之, 磯部祥子, IRINA K, 近江戸伸子, 桜井望, 佐藤修正

  2004, 日本分子生物学会年会プログラム・講演要旨集, 27th


• Development of hyper resolution FISH and physical mapping of plant genes

  Ohmido Nobuko

  Japanese Society of Breeding, 01 Dec. 2003, Breeding research, 5(4) (4), 183 - 191, Japanese


• CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetically Modified Foods : Its Effectiveness from the Viewpoints of Learners

  INAGAKI Shigenori, TAKENAKA Makiko, YAMAGUCHI Etsuji, YAMAMOTO Tomokazu, OSHIMA Jun, OSHIMA Ritsuko, MURAYAMA Isao, NAKAYAMA Hayashi, OHMIDO Nobuko, TAKESHITA Yuko

  11 Oct. 2003, 日本教育工学会大会講演論文集, 19(2) (2), 741 - 742, Japanese


• Development of hyper resolution F SH techniques and physical mapping of genes in rice.

  OHMIDO Nobuko

  30 Mar. 2002, 育種学研究 = Breeding research, 4(1) (1), 6 - 7, Japanese


• Visualization of interaction between DNA and protein molecules

  FUKUI Kiichi, FUJIWARA Shinsuke, Ohmido Nobuko

  The Japan Society of Mechanical Engineers, 01 Mar. 2002, バイオエンジニアリング講演会講演論文集, 2002(14) (14), 223 - 224, Japanese


• Estimation of nuclear DNA content of strawberry by flow cytometry.

  AKIYAMA Y., YAMAMOTO Y., OHMIDO N., OHSHIMA M., FUKUI K.

  25 Sep. 2000, 育種学研究 = Breeding research, 2(2) (2), 30 - 30, Japanese


• A high -resolution pachlytene chromosome map of Oryza sativa L. by imaging methods.

  KATO S., OHMIDO N., FUKUI K.

  25 Sep. 2000, 育種学研究 = Breeding research, 2(2) (2), 2 - 2, Japanese


• Visual detection of useful genes on plant chromosomes

  K Fukui, N Ohmido

  Jul. 2000, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 34(3) (3), 153 - 158, English


• High sensitive detection of DNA mutation by Rolling circle amplification (RCA).

  OHMIDO N., WARD David C.

  Apr. 2000, 育種学研究 = Breeding research, 2(1) (1), 84 - 84, Japanese


• Chromosome number of microspore-derived plants in cabbage and Chibese cabbage with highly regenerative ability

  KUGINUKI Y, TSUKAZAKI H, HIDA K, OHMIDO N, FUKUI K

  26 Mar. 2000, 園芸学会雑誌. 別冊, 園芸学会大会研究発表, 69(1) (1), 238 - 238, Japanese


• Quantitative Chromosome Map of Arabidopsis thaliana L.by Imaging Methods

  ITO Mikako, OHMIDO Nobuko, AKIYAMA Yukio, FUKUI Kiichi

  Arabidopsis thaliana L. has 10 chromosomes that are small and similar in morphology. Molecular cytogenetics and image analyses were applied to characterize the somatic chromosomes. Prometaphase chromosomes were quantitatively analyzed based on their patterns of condensation, because they show a prominent uneven condensation along the chromosomes. The total length of the haploid prometaphase chromosomes is 16.5 μm, which is about 1.8 times the size of the metaphase chromosomes. We also detected the 45S (18S-5.8S-25S) and 5S rDNA loci by fluorescence in situ hybridization (FISH) and determined their locations on the chromosomes by the imaging methods. As a result, a quantitative chromosome map based on the condensation pattern was developed for the first time in Arabidopsis with the locations of rDNAs by using imaging methods. It is now possible to localize genes precisely on this map.

  Japan Mendel Society, International Society of Cytology, 2000, CYTOLOGIA, 65(3) (3), 325 - 331, English


• Development of M-FISH using custom labeled nucleotide synthesis. :

  OHMIDO Nobuko, Henegariu Octavian, Ward David C

  Society of Chromosome Research, 2000, Chromosome science, 4(4) (4), 132 - 132, English


• MALE-SPECIFIC RAPD MARKERS IN Cannabis sativa CODED RETROTRANSPOSONS

  SAKAMOTO Koichi, OHMIDO Nobuko, FUKUI Kiichi, KAMADA Hiroshi, SATOH Shinobu

  Mar. 1999, Plant and cell physiology, 40, s27 - s27, English


• Analysis and utility of Chromosome information 118. Visual analysis of internal structure of rice genome by extended DNA fiber-FISH

  KIJIMA K., OHMIDO N., HIROSE T., MITSUI T., FUKUI K.

  01 Dec. 1998, 日本分子生物学会年会プログラム・講演要旨集, 21, 321 - 321, Japanese


• Detection of alien genes and genomes by molecular cytological methods.

  OOMIDO N., FUKUI K.

  Sep. 1998, 育種学最近の進歩, 40, 55 - 58, Japanese


• ESTIMATION OF COPY NUMBER BY HIGH RESOLUTION FISH USING EXTENDED DNA FIBERS IN RICE

  OHMIDO Nobuko, KIJIMA Kyoko, JONG Hans de, FUKUI Kiichi

  May 1998, Plant and cell physiology, 39, S115 - S115, English


• Paralleled changes of repetitive sequences in the Oryza genomes

  N Ohmido, K Kijimal, JH de Jong, K Fukui

  1998, CYTOGENETICS AND CELL GENETICS, 81(2) (2), 144 - 144, English

  Summary international conference


• Chromosome analysis of hybrids in the Japanese wild lily by genomic in situ hybridization

  J Miyamoto, K Fukui, N Ohmido

  1998, CYTOGENETICS AND CELL GENETICS, 81(2) (2), 132 - 132, English

  Summary international conference


• 植物染色体情報の解析・利用に関する国際ワークショップ

  近江戸 伸子, 中山 繁樹

  日本育種学会, 01 Dec. 1997, Breeding science, 47(4) (4), 410 - 410, Japanese


• 染色体情報の利用と解析に関する研究 105. DNAファイバー上でのFISH法およびその定量的解析

  木島 暁子, 近江戸 伸子, 廣瀬 玉紀, 三ツ井 敏明, 福井 希一

  Sep. 1997, 日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan, 61, 314 - 314, Japanese


• Brachyscome lineariloba 複合体 (2n=4, 8, 10, 12, 16) における r-DNAの染色体上の分布

  足立 淳子, 渡辺 邦秋, 福井 希一, 近江 戸伸子, 小菅 桂子

  Sep. 1997, 日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan, 61, 292 - 292, Japanese


• A new manual for fluorescence in situ hybridization (FISH) in plant chromosomes

  OHMIDO N.

  1996, Rice Genet Newsl.., 13, 89 - 93


• Rice chromosomes studies by fluorescence in situ hybridization with special reference to physical mapping and chromosome structure

  Ohmido Nobuko

  Hokkaido University, Sep. 1995, Journal of the Faculty of Agriculture, Hokkaido University, 66(3) (3), 277 - 320, English


• PHYSICAL MAPPING OF RICE DNAS BY AN IMPROVED FISH METHOD

  N OHMIDO, K FUKUI

  Apr. 1995, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 29(2) (2), 83 - 88, English


• Cytological studies on African cultivated rice.

  Ohmido, N, K. Fukui

  1995, Rice Biotech. Quart., 24, 20 - 20


• Involvement of transposition in dispersion of tandem repeat sequences (TrsA) in rice genomes.

  OHMIDO N.

  1994, Molec. Gen. Genet., 245, 449 - 455


• Reliable FISH method for rice chromosomes

  Ohmido, N, K. Fukui

  1993, Rice Genome, 2, 14 - 15

• Flowering genes and homeotic floral genes analysis in Jatropha in 'The Jatropha Genome'

  OHMIDO NOBUKO, Eri Makigano, Suguru Tsuchimoto, Kiichi Fukui

  Others, Springer International Publishing AG, Nov. 2017, English, Jatropha curcas is a monoecious species, which forms unisexual flowers. The unisexual flowers are produced on the same inflorescence with 10 to 30-folds higher ratio of male flowers to female flowers. The bias in the inflorescence limits Jatropha seed production. Flowering genes analysis would provide important knowledge about the multiple environmental and endogenous factors t, ISBN: 9783319496535

  Scholarly book


• Image Analysis of DNA fiber and nucleus in plants.

  OHMIDO NOBUKO, Toshiyuki Wako, Seiji Kato, Kiichi Fukui

  Joint work, Springer, Dec. 2016, English, Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play important roles in the biological systems. Development of visualization and image analysis methods are useful techniques in the analyses of the detailed structure and function

  Scholarly book


• Plant Image Analysis: Fundamentals and Applications.

  OHMIDO NOBUKO, Toshiyuki Wako, Seiji Kato, Kiichi Fukui

  Joint work, CRC Press, Jun. 2014, English

  Scholarly book


• 生物学辞典

  近江戸 伸子, 石川統ら

  Joint work, 東京化学同人, 2010, Japanese

  Scholarly book


• 植物ゲノム科学辞典

  近江戸 伸子, 駒嶺穆

  Joint work, ユニバーサルアカデミープレス, Jan. 2009, Japanese

  Scholarly book


• Dynamic and functional analysis of chromosomal proteins.

  N. Ohmido, S Uchiyama.S. Matsunaga, M. Nakagawa, T. Azuma, K. Fukui

  Joint work, Taylor and Francis, Jan. 2009, English

  Scholarly book


• Chromosome dynamics in tobacco BY-2 cultured cells.

  N. Ohmido, S. Matsunaga, K. Fukui

  Joint work, Springer-Verlag Berlin Heidelberg, Jan. 2009, English

  Scholarly book


• キーワード 人間と発達［増補改訂版］ V. 生活とテクノロジー

  丸谷 宣子, 井上 真理, 青木 務, 平山 洋介, 市橋 秀樹, 浜口 八朗, 高橋 正, 長坂 耕作, 矢野 澄雄, 福田 博也, 城 仁士, 阪本 雄二, 高橋 譲嗣, 高橋 真, 白倉 暉弘, 稲葉 太一, 白杉 直子, 近江戸 伸子, 宮田 任寿, 澤 宗則, 二宮 厚美

  Joint work, 大学教育出版, Apr. 2007, Japanese

  Textbook


• Dynamic and functional analysis of chromosomal proteins, in:"Chromosome Nanoscience and Technology (eds.) K. Fukui and T. Ushiki"

  Ohmido, N, Uchiyama, S, Matsunaga, S, Nakagawa, M, Azuma T, Fukui, K

  Joint work, Taylor and Francis, 2007, English

  Scholarly book


• Chromosome dynamics in tobacco BY-2 cultured cells, in:"Biotechnology in agriculture and forestry, 58: 51-63. (Ed. T. Nagata, K. Matsuoka, D. Inze) Tabacco NY-2 cells: From Cellular Dynamics to Omics"

  Matsunaga, S, Ohmido, N, Fukui, K

  Joint work, Springer, Jan. 2007, English

  Scholarly book


• クロモソーム植物染色体研究の方法

  OHMIDO NOBUKO, JUNKO MIYAMOTO

  Joint work, 養賢堂, Apr. 2006, Japanese, 染色体の研究に関して

  Textbook


• Comparision of Surface Structures between Extended and Condensed Stages of Barley Chromosomes Revealed with Atomic Force Microscopy

  OHMIDO, KIJIMA, HOSHI, USHIKI, FUKUI

  Joint work, Cytologia, Mar. 2005, English

  Scholarly book


• Fluorescent labeling of plant chromosomes in suspension by FISH

  MA, LEE, LI, UCHIYAMA, OHMIDO, FUKUI

  Joint work, Genes Genet. Syst., Feb. 2005, English

  Scholarly book


• Recent advances in FISH analysis of plant chromosomes

  OHMIDO, FUKUI

  Joint work, Rice Genetics, Dec. 2004, English

  Scholarly book


• Strategies for structural analysis of genomes

  SATO, S, OHMIDO, N, NAKAMURA, Y, 田畑 哲之

  Joint work, 蛋白質 核酸 酵素, Aug. 2004, Japanese

  General book


• Application of the Bio-Active Beads Method in Rice Transformation

  LIU, KAWABE, MATSUNAGA, KOBAYASHI, HARASHIMA, UCHIYAMA, OHMIDO, FUKUI

  Joint work, Plant Biotechnology, Jul. 2004, English

  Scholarly book


• High-resolution fluorescence in situ hybridization (FISH) for gene mapping and molecular analysis of rice chromosomes

  OHMIDO, FUKUI

  Joint work, Genetic diversity, 2004, English

  Scholarly book


• Recent advances in FISH analysis of plant chromosomes. In: "Advances in Rice Genetics"

  Ohmido N

  Joint work, IRRI, Jan. 2004, English

  Scholarly book


• Gene mapping

  OHMIDO, FUKUI

  Joint work, Handbook for Plant Metabolic Engineering, Apr. 2002, English

  Scholarly book


• FISH analysis

  OHMIDO, FUKUI

  Joint work, 日本生物工学会, Apr. 2002, English

  Scholarly book


• Smallness: gain and loss in plant chromosome research.

  FUKUI, OHMIDO, WAKO

  Joint work, Chromosomes Today, Dec. 2000, English

  Scholarly book


• Visual Detection of Useful Genes on Plant Chromosomes.

  FUKUI, OHMIDO

  Joint work, Japan Agricultural Research Quarterly (JARQ), Jun. 2000, English

  Scholarly book


• DNAの位置や遺伝子をカラーで見る

  OHMIDO

  Single work, 化学と生物, Jun. 2000, Japanese

  General book


• マルチカラーFISH法によるヒト染色体の核型分析．

  OHMIDO

  Single work, 化学と生物, Mar. 2000, Japanese

  General book


• Visualization of DNA sequences and gene by multi colorzation.

  OHMIDO

  Single work, Biosci. Biotech., 2000, English

  Scholarly book


• Advanced development of visualization techniques for plant genome.

  OHMIDO, FUKUI

  Joint work, Genome science protocols in plants. Plant Cell Technology series 14, 2000, English

  Scholarly book


• Recent advances in the physical mapping of genes by fluorescence in situ hybridization (FUISH) of rice.

  OHMIDO, KIJIMA, HIROSE, HANS, FUKUI

  Joint work, American Biotechnology Laboratory, Feb. 1999, English

  Scholarly book


• FISH method visualizes DNA and genes on chromosomes and genomes.

  OHMIDO, FUKUI

  Joint work, BRAIN Techno News, 1999, English

  General book


• 蛍光in situハイブリダイゼーション法を用いた分子細胞生物学

  OHMIDO, MIYAMOTO

  Joint work, クロモソーム, Mar. 1998, Japanese

  Scholarly book


• Analysis of transgene and genome by molecular cytological analysis

  OHMIDO, FUKUI

  Joint work, Recent advance in plant breeding, 1998, English

  Scholarly book


• Physical mapping of several genes in rice using fluorescent in situ hybridization.

  OHMIDO, OHTSUBO, OHTSUBO, FUKUI

  Joint work, Rice Genetics III, 1997, English

  Scholarly book

• Nano-visualization by advanced electron microscopy in chromosome research.

  Nobuko Ohmido

  Med school of Silesia syposium, Dec. 2024, English

  [Invited]

  Public discourse


• Repeatome and Cytogenetics Landscapes in Ornamental Hortensias

  Nobuko Ohmido

  Silesia Univ symposium, Dec. 2024, English

  [Invited]

  Public discourse


• Plant Chromosome Architecture: Nanoscale Observations with Cutting-Edge Microscopy..

  Nobuko Ohmido

  UEB Symposium, Dec. 2024, English

  [Invited]

  Public discourse


• 異質倍数性は野生植物の都市環境適応に有効にはたらく

  下舞陽菜, 中田泰地, 勝原光希, 加藤成二, 丑丸敦史, 近江戸伸子

  第75回染色体学会年会, Oct. 2024, Japanese


• アジサイ属の遺伝的多様性：6種のゲノムリピトームと染色体解析

  石黒更, 谷口聖太, Nicola Schmid, Tony Heitkam, Stefan Wanke, Chee How Teo, 近江戸伸子

  第74回染色体学会年会, Oct. 2023, Japanese

  Oral presentation


• Nano-Level Insights into Chromosome Biology: High-Resolution Imaging with Advanced Microscopy.

  Nobuko Ohmido

  The 8th Asia-Pacific Chromosome Colloquium (APCC8), Sep. 2023, English

  [Invited]

  Invited oral presentation


• Urban environments, chromosome formula and polyploidization in two Commelina species

  Hina Shimomai, Taichi Nakata, Koki R. Katsuhara, Seiji Kato, Atsushi Ushimaru, Nobuko Ohmido

  Plant Chromosome Biology: Cytogenetics meeting 2023, Sep. 2023, English

  Poster presentation


• Urban adaptation of Commelina communis related to polyploidization.

  Hina Shimomai, Taichi Nakata, Koki R. Katsuhara, Seiji Kato, Atsushi Ushimaru, Nobuko Ohmido

  The 8th Asia-Pacific Chromosome Colloquium (APCC8), Sep. 2023, English

  Poster presentation


• Comparative clustering analysis reveals diversity of repetitive elements in the genus Hydrangea.

  Sara Ishiguro, Shota Taniguchi, Nicola Schmid, Stefan Wanke, Tony Heitkam, Nobuko Ohmido

  TheAsia-Pacific Chromosome Colloquium (APCC8), Sep. 2023, English

  Poster presentation


• Improved CRISPR imaging techniques for target-specific DNA sequences

  Bhanu Prakash Potlapalli, Nobuko Ohmido, Houben Andreas

  The 8th Asia-Pacific Chromosome Colloquium (APCC8), Aug. 2023, English

  Oral presentation


• Unveiling the Nano-World of Plant Chromosomes: Chromatin Fiber Compaction and Scaffold Protein Localization using Advanced Microscopy Techniques.

  Nobuko Ohmido

  Plant Chromosome Biology: Cytogenetics meeting 2023, Sep. 2023, English

  Oral presentation


• Major genotype-panels for Hydrangea genomics by comparative repeat analysis.

  Sara Ishiguro, Shota Taniguchi, Nicola Schmid, Stefan Wanke, Tony Heitkam, Nobuko Ohmido

  Plant Chromosome Biology: Cytogenetics meeting 2023, Sep. 2023, English

  Poster presentation


• 在来一年生草本ツユクサの形質進化の検証 -多様な都市環境に着目して-

  中田泰地, 中濱 直之, 近江戸伸子, 丑丸敦史

  日本生態学会第70回大会, Mar. 2023, Japanese

  Poster presentation


• 科学技術の社会問題としてのゲノム編集を題材とした小学生向け教育プログラムの開発

  口羽駿平, 山口悦司, 坂本美紀, 山本智一, 原愛佳, 近江戸伸子, 俣野源晃, 澁野哲

  2022年度第5回日本科学教育学会研究会（九州・沖縄支部開催）, Feb. 2023, Japanese

  Oral presentation


• Characterization of Hydrangea repetitive sequences and analysis of satellite repeats by whole genome sequence

  Shota Taniguchi, Sara Ishiguro, Nicola Schmid, Tony Heitkam, Chee How Teo, Nobuko Ohmido

  The 73th Annual Meeting of the Society of Chromosome Research, Oct. 2022, Japanese

  Oral presentation


• Ty1-copia retrotransposons variation in Crinum thaianum (water-onion) endangered species in Thailand

  Piriya Putanyawiwa, Vipa Hongtrakul, Decha Duangnamon, Nobuko Ohmido

  The 73th Annual Meeting of the Society of Chromosome Research, Oct. 2022, English

  Oral presentation


• Topoisomerase localization and plant chromosome higher-order structure by ultra high voltage transmission electron microscope (UHVTEM).

  Nobuko OHMIDO, Channarong SARTSANGA, Rinyaporn PHENGCHAT, Toshityki WAKO, Kiichi FUKUI

  The 73th Annual Meeting of the Society of Chromosome Research, Oct. 2022, Japanese

  Oral presentation


• How do plant chromosomes adapt in animal cells?

  Nobuko Ohmido, Yeng Mun Liaw, Yikun Liu, Chee How Teo, Petr Cápal, Naoki Wada, Kiichi Fukui, Jaroslav Doležel

  The 72th Annual Meeting of the Society of Chromosome Research, Sep. 2021, Japanese

  Oral presentation


• Helium ion microscope reveals chromatin fiber compaction in barley chromosome

  Channarong SARTSANGA, Rinyaporn PHENGCHAT, Kiichi Fukui, Toshiyuki Wako, Nobuko Ohmido

  The 72th Annual Meeting of the Society of Chromosome Research, Sep. 2021, Japanese

  Poster presentation


• Helium ion microscope reveals chromatin fiber compaction in barley chromosome

  Channarong Sartsanga, Rinyaporn Phengchat, Kiichi Fukui, Toshiyuki Wako, Nobuko Ohmido

  The 23rd International Chromosome Conference, Jul. 2021

  Poster presentation


• How do plant chromosomes genetically behave and modify in human cells?

  Nobuko Ohmido

  The 23rd International Chromosome Conference, Jul. 2021, English

  Oral presentation


• Outer structure of barley (Hordeum vulgare) chromosome using Helium Ion Microscope

  Channarong Sartsanga, Rinyaporn Phengchat, Kiichi Fukui, Toshiyuki Wako, Nobuko Ohmido

  Asian Pacific Chromosome Colloquium 7, Nov. 2020, English

  Poster presentation


• Genetics and Epigenetics of Plant Chromosomes in a Human-plant Hybrid Cell Line

  Yeng Mun Liaw, Yi Kun Liu, Petr Cápal, Jaroslav Doležel, Chee How Teo, Naoki Wada, Kiichi Fukui, Nobuko Ohmido

  Asian Pacific Chromosome Colloquium 7, Nov. 2020, English

  Poster presentation


• Complex genomic and chromosome rearrangement in Human-Arabidopsis hybrid

  Liu yikun Liu YK, Liaw YM, Cápal P, Teo CH, Wada N, Fukui K, Doležel J, Ohmido N

  5th Nano- Chromosome workshop, Feb. 2020, English

  Oral presentation


• Isolation of Barley chromosome for tomography analysis

  Channarong Sartsanga, Rinyaporn Phengchat, Petr Cápal, Nobuko Ohmido

  5th Nano- Chromosome workshop, Feb. 2020, English

  [Invited]

  Oral presentation


• Insight of epigenetical chromatin dynamics in plant cell.

  Nobuko Ohmido

  5th Nano- Chromosome workshop, Feb. 2020, English

  [Invited]

  Oral presentation


• Epigenetical changes of plant cell under metal and salinity stress

  Nobuko Ohmido,Aqwin Polosoro

  財団法人染色体学会第70回年会, Sep. 2019, Japanese

  Oral presentation


• Reconstruction of Arabidopsis chromosome in human-plant hybrid cell

  Yi Kun Liu,Yeng Mun Liaw,Petr Cápal,Jaroslav Dolezel,Chee How Teo, Naoki Wada, Kiichi Fukui, Nobuko Ohmido

  財団法人染色体学会第70回年会, Sep. 2019, English

  Poster presentation


• Visualization of chromosome scaffold structure using electron tomography

  Rinyaporn Phengchat,Misa Hayashida,Nobuko Ohmido,Darren Homeniuk,Kiichi Fukui

  財団法人染色体学会第70回年会, Sep. 2019, English

  Oral presentation


• Morphology changes of rice root nucleus under iron stress.

  Aqwin Polosoro, Wening Enggarini, Toto Hadiarto, Nobuko Ohmido

  The 1st International Conference on Agriculture and Rural Development (ICARD), Aug. 2019, English

  Oral presentation


• Insights of cytogenetics research for rice genetics and breeding

  Nobuko Ohmido

  21st National Genetics Conferences, Jun. 2019, English

  [Invited]

  Invited oral presentation


• Development of nano-visualization for structural analyses of chromosome

  Channarong Sartsanga, Rinyaporn Phengchat, Kiichi Fukui, Nobuko Ohmido

  The 3rd international symposium. e-Asia meeting, Jun. 2019, English

  [Invited]

  Oral presentation


• Histone dynamics under environmental stress in plants

  Nobuko Ohmido

  The 3rd international symposium. e-Asia meeting, Jun. 2019, English

  [Invited]

  Invited oral presentation


• Visualizing environmental DNA using cytogenetics

  JO Toshiaki, MURAKAMI Hiroaki, MASUDA Reiji, OHMIDO Nobuko, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, English, Kobe Convention Center, Kobe, Japan, Domestic conference

  Oral presentation


• Epigenetical chromatin dynamics under abiotic stresses in rice.

  Nobuko Ohmido, Aqwin Polosoro

  12th International Symposium Exploring the Global Sustainability, 2019, English

  [Invited]

  Invited oral presentation


• Studies of histone modification caused by iron stress in tolerant and sensitive rice varieties

  Aqwin Polosoro, Nobuko Ohmido

  iJaDe2018, Sep. 2018, English, Kobe, Japan, International conference

  Oral presentation


• Histone dynamics of single cell in plant development and environment

  Nobuko Ohmido

  iJaDe2018, Sep. 2018, English, Kobe, Japan, International conference

  [Invited]

  Invited oral presentation


• Chromosome mapping for rice genetics and breeding

  Nobuko Ohmido

  2nd e-ASIA Symposium on Functional Nano-Biology, Sep. 2018, English, Osaka, Japan, International conference

  [Invited]

  Invited oral presentation


• Molecular Cytogenetics for Plant Genetics and Breeding

  Nobuko Ohmido

  GSA/APCC6, Jul. 2018, English, Canberra, Austraria, International conference

  [Invited]

  Invited oral presentation


• Cytological effect of histone modification and retrotransposon transcription by iron stress in rice

  Aqwin Polosoro, Wening Enggarini, Nobuko Ohmido

  GSA/APCC6, Jul. 2018, English, Canberra, Austraria, International conference

  Poster presentation


• ホウレンソウの雄決定遺伝子座周辺から見出された組換抑制領域の分子構造

  小野寺 康之, 工藤友裕, 豊田敦, 高橋光彦, 平川英樹, 鈴木穣, OHMIDO NOBUKO

  一般社団法人園芸学会平成3 0 年度春季大会, Mar. 2018, Japanese, Kindai University, Domestic conference

  Poster presentation


• Transformant propagation and chromosome research in Jatropha

  OHMIDO NOBUKO, Yukon Liu, Rumi Matuoka, Naoki Wada, Suguru Tsuchumoto, Hisashi Tomemori, Hisashi Tsujimoto

  鳥取大学乾燥地研究センター共同研究発表会, Dec. 2017, Japanese, Tottori University, It is difficult to obtain fluorescence images and analyze the localizations of nucleic acid and/or protein in plant cells keeping the structure of plant organs. The purpose of our study is analyze the transgene localization in Jatropha genome using FISH and high-sensitive 3D imaging technique in inner tissues structures., Domestic conference

  Poster presentation


• Effective chromosome research for inedible biomass plants

  OHMIDO NOBUKO

  10th International symposium Exploring the global sustainability, Mar. 2017, English, Osaka university, In this study, we investigated polyploidy level and chromosome structure using chromosome morphological analysis, fluorescence in situ hybridization (FISH), and chromosome image analyzing system (CHIAS) in Kudzu and Pyrethrum., International conference

  Oral presentation


• High sensitive 3D imaging of immunostaining in plants tissue

  松岡留美, OHMIDO NOBUKO

  財団法人日本染色体学会 第67回年会, Nov. 2016, Japanese, Tokyo university, Using high-sensitive 3D imaging technique, more clearly plant inner structures are investigated. This technique will be combined the immunostaing using antibodies against proteins and DNA- or RNA-FISH in 3D structure cell. We expect that this technique could visualize in vivo localization of small molecules in cells and help to understand plant functions, Domestic conference

  Oral presentation


• Chromosome research of Pyrethrum

  OHMIDO NOBUKO, 里山昌子, 松田一彦

  財団法人日本染色体学会 第67回年会, Nov. 2016, Japanese, Tokyo university, A cultivar of Pyrethrum Tanacetum cinerariifolium in Asteraceae is used for the natural insecticide product, Pyrethrin for long time ago, before the industrial chemical agents Pyrethroid were pervasive. However, its genetics study has just barely started. In previous research by Watanabe1), most Tanacetum are composed diploid species having 2n=2x=18 with the basic chromosome nu, Domestic conference

  Oral presentation


• Perspective of effective plant usages for edible and energy plants

  OHMIDO NOBUKO

  German-Japanese Symposium, Bridge Dresden-Japan, May 2016, English, Technische Universität Dresden, International conference

  [Invited]

  Invited oral presentation


• Chromatin modification and mobility in plant development

  OHMIDO NOBUKO

  German-Japanese Symposium, Bridge Dresden-Japan, May 2016, English, The Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany, International conference

  [Invited]

  Invited oral presentation


• Transgenic flowering gene and chromosome research in Jatropha

  Ayaka Hata, Suguru Tsuchimoto, Kiichi Fukui, Hisashi Tomemori, Hisashi Tsujimoto, OHMIDO NOBUKO

  10th International symposium Exploring the global sustainability, Mar. 2016, English, Osaka university, In this study, we have expected early flowering and were intended to contribute to the effect Jatropha breeding. We performed three experimental trails. First, we constructed explants introduced Flowering Locus T gene and established the transformation and effective culture method., International conference

  Oral presentation


• Diversity of ribosomal DNA and chromosome conformation gene in Camellia

  小川桜子, 杉山 昇平, 飯田 誠也, 太田 智也, OHMIDO NOBUKO, 古川 一実

  129th Breeding Science, Mar. 2016, Japanese, Yokohama city Univ, チャ（Camellia sinensis，2n=30）の変異拡大を目的とした近縁種との交雑における染色体の挙動解析のために，基本的な核型分析および減数分裂の対合を観察した．まずランドマークとして用いるリボゾーム遺伝子の多様性および染色体構成を調査するために，rDNAシークエンスおよび染色体fluorescence in situ hybridization(FISH)を比較した．またCamellia属植物3種において，チャカフェイン合成酵素遺伝子(TCS1)の染色体上での位置の特定を試みた．, Domestic conference

  Oral presentation


• Chromosome Study of Pyrethrum

  Masako Satoyama, Koji Sakamori, Kazuhiko Matsuda, Kenji Taniguchi, OHMIDO NOBUKO

  II International Symposium on Pyrethrum, Aug. 2015, English, Kyoto, Japan, A cultivar of Tanacetum cinerariifolium, Pyrethrum in Asteraceae is used for the natural insecticide product, Pyrethrin for long period before the industrial chemical agents. However, its genome study has just barely started. In this study, we investigated polyploidy level and chromosome structure using chromosome morphological analysis, fluorescence in situ hybridization (FISH, International conference

  Poster presentation


• Research of early flowering transgenic Jatropha plant

  Ayaka Hata, Suguru Tsuchumoto, Kiichi Fukui, Hisashi Tomemori, Hisashi Tsujimoto, OHMIDO NOBUKO

  Asia Chromosome Colloquium, May 2015, English, Bangkok, Thai lands, Jatropha curcas L is a monoecious plant. But the ratio of female flower is less than the male flower. So the productivity falls and the yield is limited. So the study of a flower is important to improve epiphytic of fruit and yield. In this study, we generated early flowering transgenic Jatropha by introducing FLOWERING LOCUS T (FT) gene which is flowering hormone using agrobac, International conference

  Poster presentation


• Chromosome instability of allopolyploid resynthesized Brassica napus

  OHMIDO NOBUKO, Kanako UEDA, Kouei FUJII, Kiyotaka NAGAKI, Minoru MURATA

  Asia Chromosome Colloquium, May 2015, English, Bangkok, Thai lands, A new cultivar of Brassica napus “Hanakkori” (AACC, 2n = 38) was resynthesized by hybridization and diplodization between B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18) in Yamaguchi, Japan. However, its genome instability has been noticed as shown in other resynthesized B. napus cultivars. In fact, when out-crossed seeds of Hanakkori are germinated, various kinds of morpho, International conference

  [Invited]

  Nominated symposium


• Research of early flowering transgenic Jatropha plant

  OHMIDO NOBUKO, Ayaka Hata, Suguru Tsuchimoto, Kiichi Fukui, Hisashi Tomemori, Hisashi Tsujimoto

  8th International Symposium Exploring the global sustainability-Advances in Plant Biotechnology for Agriculture in Semi-arid land-, Mar. 2015, English, Osaka Univ, International conference

  Oral presentation


• Camellia属植物および種間雑種におけるrDNA配列FISHの比較

  OHMIDO NOBUKO, 古川一実, 飯田誠也, 寺前香里, 小川桜子

  127th Breeding Science, Mar. 2015, Japanese, Tamagawa Univ, Domestic conference

  Oral presentation


• 系統的に異なるSpinacia属植物からの同型および異型性染色体の同定

  OHMIDO NOBUKO, 藤戸 聡史, 鈴木 伶実, 星野 洋一郎, 小野寺 康之

  37th Japan Moleculat Biology, Nov. 2014, Japanese, yokohama, Domestic conference

  Oral presentation


• 画像解析によるイネ染色体統合地図の構築

  OHMIDO NOBUKO, 若生俊行, 加藤成二, 岩田愛子, 福井希一

  65th Chromosome Science, Oct. 2014, Japanese, Okayama Univ, Domestic conference

  Oral presentation


• Chromosome instability in the progeny of amphidiploid resynthesized Brassica napus

  OHMIDO NOBUKO, 植田加奈子, 藤井宏栄, 長岐清孝, 村田稔

  65th Chromosome Science, Oct. 2014, English, Okayama Univ, Domestic conference

  Poster presentation


• サトウキビとエリアンサスの属間雑種の細胞遺伝学的特性および農業形質の評価

  OHMIDO NOBUKO, バビル パチャキル, 寺島義文, 伊禮信, 高木洋子

  125th Breeding Science, Mar. 2014, Japanese, Tohoku Univ., Domestic conference

  Oral presentation


• Spinacia属から見出された同型および異型性染色体のFISH法による特徴付け

  OHMIDO NOBUKO, 藤戸 聡史, 鈴木 伶実, 星野 洋一郎, 小野寺 康之

  125th Breeding Science, Mar. 2014, Japanese, Tohoku, Domestic conference

  Oral presentation


• Generation of early flowering transgenic plant and study of flower development in Jatropha.

  OHMIDO NOBUKO

  7th meeting of international society for environmental bio-Resources, Mar. 2014, English, Osaka Univ, International conference

  [Invited]

  Invited oral presentation


• Divergence of plant genome revealed by FISH

  OHMIDO NOBUKO

  Chromosome imaging workshop, Feb. 2014, English, Oxford Univ.UK., International conference

  [Invited]

  Invited oral presentation


• ジャトロファの早期開花組換えならびに花成に関する研究

  OHMIDO NOBUKO

  平成２５年度鳥取大学乾燥地研究センター共同研究発表会, Dec. 2013, Japanese, 鳥取大学, Domestic conference

  Poster presentation


• 日本に自生するクズの核型解析

  OHMIDO NOBUKO, Akane Shimoura, 加藤成二, 磯部祥子, 田畑哲之

  染色体学会, Nov. 2013, Japanese, 財団法人染色体学会, 富山大学, Domestic conference

  Oral presentation


• Production of transgenic plants with early flowering gene and morphological analysis of flowers in Jatoropha

  OHMIDO NOBUKO

  6th International Symposium Green Biotechnology for Global Sustainability, Mar. 2013, English, Osaka Univ, International conference

  Oral presentation


• 油糧植物ジャトロファの早期開花組換え体創出に関する研究

  OHMIDO NOBUKO

  鳥取大学乾燥地研究センター発表会, Dec. 2012, Japanese, 鳥取大学乾燥地研究センター, Domestic conference

  Poster presentation


• バイオ燃料植物ジャトロファの開花遺伝子ならびに組換えに関する研究

  近江戸 伸子, 井本 衣里, 福井 希一

  日本育種学会, Nov. 2012, Japanese, 京都産業大学, Domestic conference

  Oral presentation


• Development of Jatropha transgenic plants with drought tolerance.

  OHMIDO NOBUKO

  International Conference on NextGeneration Technologies for Bioenergy and Biomass Utilisation, Aug. 2012, English, Singapore, International conference

  [Invited]

  Invited oral presentation


• アーギュメンテーション・スキルの育成を目指した実践的研究:小学校第6学年の理科授業デザイン

  村津 啓太, 山本 智一, 稲垣 成哲, 近江戸 伸子, 坂本 美紀, 山口 悦司, 神山 真一

  平成23年度日本理科教育学会近畿支部大会発表論文集, Nov. 2011, Japanese, Domestic conference

  Oral presentation


• 大学職場の男女平等実現のためのストラテジー

  朴木 佳緒留, 近江戸 伸子, 川島 弓枝

  2008年度日本女性学会大会, Jun. 2008, Japanese, アピオ青森, Domestic conference

  Oral presentation


• アカクローバー完全染色体地図の構築

  片岡 遼平, 原 昌輝, 加藤 成二, 磯部 祥子, 佐藤 修正, 田畑 哲之, 近江戸 伸子

  第５８回染色体学会, Nov. 2007, Japanese, 総合研究大学院大学 葉山キャンパス, Domestic conference

  Oral presentation


• CHIAS4を用いたアカクローバー染色体の画像解析法の確立

  原 昌輝, 片岡 遼平, 加藤 成二, 磯部 祥子, 佐藤 修正, 田畑 哲之, 近江戸 伸子

  第５８回染色体学会, Nov. 2007, Japanese, 総合研究大学院大学 葉山キャンパス, Domestic conference

  Oral presentation


• 学校と専門家を結ぶケータイと電子掲示板を使った連携授業：小学校2年生の生活科における冬野菜の栽培活動

  竹中 真希子, 稲垣 成哲, 黒田 秀子, 大久保 正彦, 出口 明子, 近江戸 伸子

  日本教育工学会第23回, Sep. 2007, Japanese, 早稲田大学, Domestic conference

  Oral presentation


• Quantitative and fine rice pachytene chromosome map by analyzed chromomeric features

  Aiko Iwata, Seiji Kato, Kiichi Fukui, Nobuko Ohmido

  16th International Chromosome Conference, Aug. 2007, English, Amsterdam, International conference

  Poster presentation


• Legume chromosome studies of Trifolium pretense and Lotus japonicus

  Nobuko Ohmido, Seiji Kato, Ryohei Kataoka, Akiko Ishimaru, Sachiko Isobe, Shusei Sato, Satoshi Tabata

  16th International Chromosome Conference, Aug. 2007, English, 16th International Chromosome Conference, Amsterdam, International conference

  Poster presentation


• Chromosome map analysis of Red clover

  Ryohei Kataoka, Masaki Hara, Seiji Kato, Sachiko Isobe, Shusei Sato, Satoshi Tabata, Nobuko Ohmido

  The 5th International Symposium on the Molecular Breeding of Forage and Turf, Aug. 2007, English, Sapporo, International conference

  Poster presentation


• マメ科植物染色体の定量的マッピングの進展

  片岡 遼平, 原 昌輝, 加藤 成二, 磯部 祥子, 佐藤 修正, 田畑 哲之, 近江戸 伸子

  染色体学会第57回大会, Nov. 2006, Japanese, 千葉大学, Domestic conference

  Oral presentation


• カプセル化法を用いた植物培養細胞の凍結保存

  田中 美佳, 近江戸 伸子

  染色体学会第57回大会, Nov. 2006, Japanese, 千葉大学, Domestic conference

  Oral presentation


• アカクローバーにおける連鎖地図と染色体地図の統合

  片岡 遼平, 原 昌輝, 加藤 成二, 磯部 祥子, 佐藤 修正, 田畑 哲之, 近江戸 伸子

  染色体学会第57回大会, Nov. 2006, Japanese, 千葉大学, Domestic conference

  Oral presentation


• タバコならびにイネ培養細胞のカプセル化による効果的な凍結保存法

  田中 美佳, 近江戸 伸子

  日本育種学会第110回大会, Sep. 2006, Japanese, 愛媛大学, Domestic conference

  Oral presentation


• 遺伝子組み換え食品を題材にしたCSCLシステム活用型科学教育カリキュラム：実験授業に参加した科学者が語るカリキュラムの意義

  藤本 雅司, 稲垣 成哲, 近江戸 伸子, 橘 早苗, 山本 智一, 山口 悦司, 竹中 真希子, 大島 純, 村山 功, 中山 迅, 大島 律子, 坂本 美紀

  平成１７年度日本理科教育学会近畿支部大会, Nov. 2005, Japanese, 滋賀大学, Domestic conference

  Oral presentation


• ケータイを活用した学校・農園連携授業の試み：小学校２年生の生活科「冬の野さいをそだてよう」

  稲垣 成哲, 近江戸 伸子, 黒田 秀子, 山下 真, 一藁 章敬, 山中 康広, 大久保 正彦, 出口 明子, 竹中 真希子

  平成１７年度日本理科教育学会近畿支部大会, Nov. 2005, Japanese, 滋賀大学, Domestic conference

  Oral presentation


• CSCL-based Science Curriculum on the Theme of Social Decision-Making about Genetic Modification Foods: Can Pupils Transfer their Decision-Making to Other Issue?

  SAKAMOTO, Miki, 藤本 雅司, INAGAKI, Shigenori, YAMAGUCHI, Etsuji, TAKENAKA, Makiko, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, OHMIDO, Nobuko, 橘 早苗, 山本 智一

  日本科学教育学会第29回年会, Sep. 2005, Japanese, 日本科学教育学会, 岐阜, 日本, Domestic conference

  Oral presentation


• CSCL-based Science Curriculum on the Theme of Social Decision-Making about Genetic Modification Foods: Achievement of Social Decision-Making of Elementary School Child Who Studied about the 2004 Version of the Curriculum

  FUJIMOTO Masaji, SAKAMOTO, Miki, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, OHMIDO, Nobuko, 橘 早苗, 山本 智一

  日本科学教育学会第29回年会, Sep. 2005, Japanese, 日本科学教育学会, 岐阜, 日本, Domestic conference

  Oral presentation


• Integration of the quantitative chromosome map and linkage map of Lotus japonicus.

  ISHIMARU, KATAOKA, TABATA, SATOH, FUKUI, OHMIDO

  日本育種学会 第107・108回講演会, Aug. 2005, Japanese, 日本育種学会, Tsukuba, 日本, Domestic conference

  Invited oral presentation


• ITによる協調学習支援ー自然・社会認識におけるITを活用した協調学習の授業デザイン

  黒田 秀子, 神山 真一, 山本 智一, 橘 早苗, 稲垣 成哲, 蛯名 邦禎, 近江戸 伸子, 大久保 正彦, 武田 義明, 田結庄 良昭, 鳩野 逸生, 望月 俊男, 大島 純, 大島 律子, 小石 寛文, 坂本 美紀, 竹中 真希子, 土井 捷三, 中山 迅, 舟生 日出男, 村山 功, 山口 悦司

  平成１７年度学部附属共同研究教育研究発表会, Jun. 2005, Japanese, 神戸大学発達科学部附属住吉小学校, Domestic conference

  Others


• Knowledge Forumを利用した学習環境のデザイン実験：遺伝子組換え食品を題材にした単元の開発III

  FUJIMOTO, Masaji, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKYAMA, Hayashi, SAKAMOTO, Miki, OHMIDO, Nobuko, YAMAMOTO, Tomokazu, TACHIBANA, Sanae, TAKESITA, Yuko

  平成16年度日本理科教育学会近畿支部大会, Nov. 2004, Japanese, 日本理科教育学会近畿支部, 大阪, 日本, Domestic conference

  Poster presentation


• CSCLシステムを利用した授業のデザイン実験

  稲垣 成哲, 山本 智一, 黒田 秀子, 橘 早苗, 竹下 裕子, 藤本 雅司, 大島 純, 中山 迅, 山口 悦司, 村山 功, 竹中 真希子, 大島 律子, 舟生 日出男, 出口 明子, 鈴木 栄幸, 加藤 浩, 大久保 正彦, 武田 義明, 田結庄 良明, 小石 寛文, 土井 捷三, 伊東 昌子, 坂本 美紀, 鳩野 逸生, 五十里 美和, 望月 俊男, 小川 正賢, 近江戸 伸子

  日本科学教育学会研究会研究報告, Oct. 2004, Japanese, 日本科学教育学会, Domestic conference

  Oral presentation


• Localization of Heterochromatin Protein 1 and meilated HistoneH3 in plant cell cycles.

  NO, NAKAGAWA, KAWABE, MATUNAGA, FUKUI, OHMIDO

  日本遺伝学会 第76会大会, Sep. 2004, Japanese, 日本遺伝学会, Suita, 日本, International conference

  Oral presentation


• Intercelluler dynamics of heterochromatin protein in BY-2 cell cycle.

  NO, NAKAGAWA, KAWABE, MATUNAGA, FUKUI, OHMIDO

  International workshopCell and Molecular Biology of Tobacco BY-2 cells BY 2, Sep. 2004, English, Yokohama, 日本, International conference

  Poster presentation


• A quantitative pachytene chromosome mapping of Lotus japonicus using FISH and CHIAS3

  ISHIMARU, TABATA, SATO, HAYASHI, HASHIMOTO, FUKUI, OHMIDO

  日本育種学会 第106回講演会, Sep. 2004, Japanese, 日本育種学会, Tsu, 日本, Domestic conference

  Oral presentation


• CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetic Modification Foods : Analyses of Achievement of Social Decision-Making

  FUJIMOTO, Masaji, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, YAMAMOTO, Tomokazu, SAKAMOTO, Miki, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKYAMA, Hayashi, OHMIDO, Nobuko, TAKESITA, Yuko

  日本科学教育学会第28回年会, Aug. 2004, Japanese, 日本科学教育学会, 千葉, 日本, Domestic conference

  Oral presentation


• CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetic Modification Foods : Analysis of Students' Understanding of Basic Contents

  山本 智一, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, 藤本 雅司, SAKAMOTO, Miki, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKYAMA, Hayashi, OHMIDO, Nobuko, TAKESITA, Yuko

  日本科学教育学会第28回年会, Aug. 2004, Japanese, 日本科学教育学会, 千葉, 日本, Domestic conference

  Oral presentation


• CSCL Environment for Science Education on the Theme of Social Decision-Making about Genetically Modified Foods : How Students Change Their Conceptual Understanding and Image

  SAKAMOTO, Miki, INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, 藤本 雅司, 山本 智一, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKYAMA, Hayashi, OHMIDO, Nobuko, TAKESITA, Yuko

  日本科学教育学会第28回年会, Aug. 2004, Japanese, 日本科学教育学会, 千葉, 日本, Domestic conference

  Oral presentation


• High resolution chromosome mapping of Lotus japonicus

  OHMIDO, ISHIMARU, SAKAGUCHI, HAYASHI, SATOH, TABATA, FUKUI

  The 2nd asian chromsome colloquium, May 2004, English, 太田, 韓国, International conference

  Oral presentation


• 遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境：学習者からみた有効性

  INAGAKI, Shigenori, TAKENAKA, Makiko, YAMAGUCHI, Etsuji, YAMAMOTO, Tomokazu, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, OHMIDO, Nobuko, TAKESHITA, Yuko

  日本教育工学会第19回全国大会, Oct. 2003, Japanese, 日本教育工学会, 盛岡, 日本, Domestic conference

  Oral presentation


• Physical mapping of Lotus japonicus by repetitive sequences and TAC clones

  ISHIMARU, SAKAGUCHI, SATOH, TABATA, HAYASHI, FUKUI, OHMIDO

  日本育種学会 第104回講演会, Sep. 2003, Japanese, 日本育種学会, Kobe, 日本, Domestic conference

  Oral presentation


• Knowledge Forumを利用した学習環境のデザイン実験：遺伝子組み換え食品を題材にした単元の開発II

  YAMAMOTO, Tomokazu, TAKENAKA, Makiko, INAGAKI, Shigenori, YAMAGUCHI, Etsuji, OSHIMA, Jun, OSHIMA, Ritsuko, MURAYAMA, Isao, NAKAYAMA, Hayashi, OHMIDO, Nobuko, TAKESHITA, Yuko

  日本理科教育学会第53回全国大会, Aug. 2003, Japanese, 日本理科教育学会, 札幌, 日本, Domestic conference

  Oral presentation


• High resolution FISH for gene mapping and molecular analysis of rice chromosomes

  OHMIDO, FUKUI

  The 1st asian chromsome colloquium, Sep. 2001, English, Beijing, 中国, International conference

  Oral presentation


• 遺伝子高感度可視化技術によるゲノム解析

  OHMIDO

  東京大学分子細胞生物学研究所セミナー, Mar. 2001, Japanese, 東京大学, Tokyo, 日本, Domestic conference

  Invited oral presentation


• Genome size of the genus Fragaria

  AKIYAMA, YAMAMOTO, OHMIDO, OHSHIMA, FUKUI

  Plant and Animal Genome IX, Jan. 2001, English, Schergo International,Inc, SanDiego, アメリカ, International conference

  Poster presentation


• 高感度可視化技術を用いた遺伝子の検出

  OHMIDO

  第10回 染色体コロキュウム, Dec. 2000, Japanese, かずさDNA研究所, 木更津, 日本, Domestic conference

  Invited oral presentation


• Visualization of genes by FISH method in plant genome

  OHMIDO

  第23回 日本分子生物学会年会, Dec. 2000, Japanese, 日本分子生物学会, Kobe, 日本, Domestic conference

  Oral presentation


• カスタムの標識ヌクレオチドを用いたマルチカラーFISH法の開発

  OHMIDO, HENEGARIU, WARD

  第51回 染色体学会, Oct. 2000, Japanese, （財）染色体学会, Yokohama, 日本, Domestic conference

  Oral presentation


• High resolution FISH for gene mapping and molecular analysis of rice chromosomes

  OHMIDO, FUKUI

  4th International Rice Genetics Symposium, Oct. 2000, English, International Rice Research Insutitute, Laguna, フィリピン, International conference

  Oral presentation


• A high-resoiution pachytene chromosome map of Oryza sativa L. by imaging methods.

  KATOH, OHMIDO, FUKUI

  日本育種学会 第98回講演会, Sep. 2000, Japanese, 日本育種学会, Hirosaki, 日本, Domestic conference

  Oral presentation


• Estimation of nuclear DNA content of strawberry by fiow cytometry.

  AKIKYAMA, Y, YAMAMOTO, Y, OHMIDO, N, OHSHIMA, M, FUKUI, K

  日本育種学会 第98回講演会, Sep. 2000, Japanese, 日本育種学会, Hirosaki, 日本, Domestic conference

  Oral presentation


• 遺伝子の可視化ー染色体から１塩基まで

  OHMIDO

  大阪大学大学院工学研究科応用生物セミナー, Jul. 2000, Japanese, 大阪大学, Suita, 日本, Domestic conference

  Invited oral presentation


• High sensitive detection of DNA mutation by Rolling circle amplification(RCA).

  OHMIDO, WARD

  日本育種学会 第97回講演会, Apr. 2000, Japanese, 日本育種学会, Tsukuba, 日本, Domestic conference

  Oral presentation


• Chromosome number of microspore-derived plants in cabbabe and Chibese cabbbage with highly regenerative ability.

  KUGINUKI, TSUKAZAKI, HIDA, OHMIDO, FUKUI

  園芸学会 平成12年度春季大会研究発表, Mar. 2000, Japanese, 園芸学会, Kobe, 日本, Domestic conference

  Oral presentation


• BRYOPHYTE 5S RDNA WAS INSERTED INTO 45S RDNA REPEAT UNITS AFTER THE DIVERGENCE FROM HIGHER LAND PLANTS

  SONE, T, FUJISAWA, M, TAKENAKA, M, NAKAGAWA, S, YAMAOKA, S, OHMIDO, N

  Plant and Animal Genome VIII, Jan. 2000, English, Scherago International,Inc., SanDiego, アメリカ, Domestic conference

  Poster presentation


• 植物染色体をのばしてみる-染色体FISHからDNAファイバーFISHへ-

  MUKAI, Y, OHMIDO, N, YAMAMOTO, M

  染色体学会 第50回記念大会, Nov. 1999, Japanese, （財）染色体学会, Nishinomiya, 日本, Domestic conference

  Oral presentation


• Physical mapping of ribosomal RNA genes in Nicotiana tabacum L. and its relatives.

  KITAMURA, INOUE, OHMIDO, FUKUI

  日本育種学会 第96回講演会, Sep. 1999, Japanese, 日本育種学会, Okayama, 日本, Domestic conference

  Oral presentation


• Analysis and utility of cromosome information by scanning grobe microscopy.1.Applocation of atomatic force microscopy to extended DNA fiber samples.

  HIROSE, OHMIDO, FUKUI, OHTANI

  日本育種学会 第95回講演会, Apr. 1999, Japanese, 日本育種学会, Utsunomiya, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome information 119.Development of a quantitative chromosome map of Arabidopsis by imaging methods.

  ITO, OHMIDO, AKIYAMA, KOBA, FUKUI

  日本育種学会 第95回講演会, Apr. 1999, Japanese, 日本育種学会, Utsunomiya, 日本, Domestic conference

  Oral presentation


• Structual analysis for a YAC clone orginating from the centromeric region of rice

  NONOMURA, OHMIDO, FUKUI, KURATA

  日本育種学会 第95回講演会, Apr. 1999, Japanese, 日本育種学会, Utsunomiya, 日本, Domestic conference

  Oral presentation


• Chromosommorphorogical characteristics of hexaploid somatic hybrids between Solanum integrifolium and S.sane composition and S.sanitwongsei.

  IWAMOTO, HIRAI, OHMIDO, FUKUI

  日本育種学会 第95回講演会, Apr. 1999, Japanese, 日本育種学会, Utsunomiya, 日本, Domestic conference

  Oral presentation


• Identification of small chromosomes of Nicotiana Kawakamii Y.Ohashi by fiuorescence in situ hybridization with 5SrDNA.

  NAKAMURA, KITAMURA, INOUE, OHMIDO, FUKUI

  日本育種学会 第95回講演会, Apr. 1999, Japanese, 日本育種学会, Utsunomiya, 日本, Domestic conference

  Oral presentation


• アラビドプシスにおける染色体の定量的地図の作成

  OHMIDO, ITO, KOBA, FUKUI

  第16回 染色体ワークショップ, Jan. 1999, Japanese, 日本,, Domestic conference

  Oral presentation


• Visualization of the DNA stretches in a rice genome by extended DNA fiber-FISH.

  KIJIMA, OHMIDO, HIROSE, MITSUI, FUKUI

  Plant and Animal Genome VII, Jan. 1999, English, Scherago International,Inc., SanDiego, アメリカ, International conference

  Oral presentation


• Development of a quantitative chromosome map of Arabidopsis by imaging methods.

  ITO, OHMIDO, KOBA, FUKUI

  Plant and Animal Genome VII, Jan. 1999, English, Scherago International,Inc., SanDiego, アメリカ, International conference

  Oral presentation


• A new method in optical mapping visualizes the structure of the S locus region of Brassica campestris L.

  HIROSE, KIJIMA, OHMIDO, SUZUKI, KAI, WATANABE, ISOGAI, HINATA, FUKUI

  Plant and Animal Genome VII, Jan. 1999, English, Scherago International,Inc., SanDiego, アメリカ, International conference

  Oral presentation


• Analysis and utility of chromosome information 118.Visual analysis of internal structure of rice benome by extended DNA fiber-FISH

  KIJIMA, OHMIDO, HIROSE, MITSUI, FUKUI

  第21回 日本分子生物学会年会, Dec. 1998, Japanese, 日本分子生物学会, Yokohama, 日本, Domestic conference

  Poster presentation


• Analysis and utility of chromosome information 117.Karyotype analysis of spinach chromosomes by condensation patterns.

  ITOU, KOBA, AKIYAMA, OHMIDO, FUKUI

  日本育種学会 第94回講演会, Sep. 1998, Japanese, 日本育種学会, Morioka, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome information 116.Visualmapping on single DNA molecule by DNA combing method.

  HIROSE, KIJIMA, OHMIDO, SUZUKI, KAI, WATANABE, HINATA

  日本育種学会 第94回講演会, Sep. 1998, Japanese, 日本育種学会, Morioka, 日本, Domestic conference

  Oral presentation


• Comparative analysis of T genome chromosomes in Nicotiana tabacum L. and its related species.

  KITAMURA, INOUE, OHMIDO, FUKUI

  日本育種学会 第94回講演会, Sep. 1998, Japanese, 日本育種学会, Morioka, 日本, Domestic conference

  Oral presentation


• Paralleled changes of repetitive sequences in the Oryza genomes.

  OHMIDO, KIJIMA, HANS, FUKUI

  13th International Chromosome Conference, Sep. 1998, English, Cytogenetics and Cell Genetics, Ancona, イタリア, International conference

  Poster presentation


• Comparison of ribosomal DNA loci on chromosomes between cultivated soybean and wild soybean by FISH

  FUJII, OHMIDO, AKIYAMA, FUKUI, SHIMAMOTO

  日本育種学会 第94回講演会, Sep. 1998, Japanese, 日本育種学会, Morioka, 日本, Domestic conference

  Oral presentation


• Karyotype analysis of Nicotiana kawakamii Y.Ohashi by CMA/DAPI stsining and FISH.

  NAKAMURA, KITAMURA, INOUE, OHMIDO, FUKUI

  日本育種学会 第94回講演会, Sep. 1998, Japanese, 日本育種学会, Morioka, 日本, Domestic conference

  Oral presentation


• Visual verification of the telomeric structure of the rice chromosomes

  FUKUI, OHMIDO, HANS

  The 18th International Congress of Genetics, Aug. 1998, English, Chinese Academy of Sciences, Genetics Society of China, Beijing, 中国, International conference

  Oral presentation


• イネの伸長DNAファイバーを用いた高解像FISH法による遺伝子コピー数の推定

  OHMIDO, KIJIMA, HANS, FUKUI

  日本植物生理学会 1998年度年会, May 1998, Japanese, 日本植物生理学会, Hokkaodo, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome information 114. Development of quantitative analysis of copy number of genes EDFs-FISH.

  FUKUI, OHMIDO

  日本育種学会 第93回講演会, Apr. 1998, Japanese, 日本育種学会, Yokohama, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome information 113.Visualization of the single copy benes in Brassica campestris L.by EDFs-FISH.

  HIROSE, KIJIMA, OHMIDO, SUZUKI, WATANABE, HINATA, FUKUI

  日本育種学会 第93回講演会, Apr. 1998, Japanese, 日本育種学会, Yokohama, 日本, Domestic conference

  Oral presentation


• Characteristics and chromosome aberration of the F2 generation in Festuca-Lolium interbeneric hybridization.

  MIZUKAMI, SUGITA, HOUMURA, OHMIDO, FUKUI

  日本育種学会 第93回講演会, Apr. 1998, Japanese, 日本育種学会, Yokohama, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome information 110. Detection of specific chromosome reduction in rice somatic hybrids with A,B and C genome by multi-color genomic in situ hybridization.

  SHISHIDO, R, SOMSAK, A, OHMIDO, N, OKINAKA, Y, MORI, K, SANO, Y, FUKUI, K

  Plant and Animal Genome VI, Jan. 1998, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Oral presentation


• Analysis and utility of chromosome information 109. Molecular cytogenetical characteristics of rice tissue culture cells with high activity of retrotransposon.

  NAKAYAMA, S, HIROCHIKA, H, OHMIDO, N, FUKJUI, K

  Plant and Animal Genome VI, Jan. 1998, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Oral presentation


• Advances in high resolution FISH using extended DNA fibers in plants.

  OHMIDO, HANS, FUKUI

  Plant and Animal Genome VI, Jan. 1998, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Oral presentation


• Analysis and utility of chromosome information 108.Identification of rice chromosomes by Spectra-FISH.

  NAKAYAMA, MIYAMOTO, OHMIDO, WING, FUKUI

  第20回 日本分子生物学会年会, Dec. 1997, Japanese, 日本分子生物学会, Kyoto, 日本, Domestic conference

  Poster presentation


• Analysis and utility of chromosome information 107.Visualization of rRNA gene in Brassica campestris L. by EDF/FISH method.

  HIROSE, KIJIMA, WAKO, OHMIDO, FUKUI

  日本育種学会 第92回講演会, Oct. 1997, Japanese, 日本育種学会, Tottori, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome informatio 106. Development of quantitative chromosome maps by using Java.

  KATO, AKIYAMA, HIROSE, OHMIDO, FUKUI

  日本育種学会 第92回講演会, Oct. 1997, Japanese, 日本育種学会, Tottori, 日本, Domestic conference

  Oral presentation


• Analysis of signal patterns obtained by genomic in situ hybridization in interspecific hybrids between Nicotiana tabacum L. and N. kawakamii Y. Ohashi.

  NAKAMURA, KITAMURA, INOUE, OHMIDO, FUKUI

  日本育種学会 第92回講演会, Oct. 1997, Japanese, 日本育種学会, Tottori, 日本, Domestic conference

  Oral presentation


• 染色体情報の利用と解析に関する研究105. DNAファイバー不でのFISH法およびその定量的解析．

  KIJIMA, OHMIDO, HIROSE, MITSUI, FUKUI

  日本植物学会 第61回大会研究発表, Sep. 1997, Japanese, 日本植物学会, Narashino, 日本, Domestic conference

  Oral presentation


• RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and progenies between Lycopersicon esculentum and Solanum lycopersicoides.

  ESCALANTE, R A, IMANISHI, S, OHMIDO, N, FUKUI, K

  5th International Congress of Plant Molecular Biology, Sep. 1997, English, The International Society For Plant molecular Bioligy,The National Univercity Of singapore,The Insutitute Of Molecular Agrobiology, Singapore, シンガポール, International conference

  Poster presentation


• Physical and Quantitative localisation of repetitive sequences in Oryza Species.

  OHMIDO, KIJIMA, ASHIKAWA, HANS, FUKUI

  5th International Congress of Plant Molecular Biology, Sep. 1997, English, The International Society For Plant molecular Bioligy,The National Univercity Of singapore,The Insutitute Of Molecular Agrobiology, Singapore, シンガポール, International conference

  Poster presentation


• Localization of rice 5S-rRNA genes on extended DNA fibers.

  FUKUI, OHMIDO, KIJIMA

  5th International Congress of Plant Molecular Biology, Sep. 1997, English, The International Society For Plant molecular Bioligy,The National Univercity Of singapore,The Insutitute Of Molecular Agrobiology, Singapore, シンガポール, International conference

  [Invited]

  Invited oral presentation


• Brachyscome lineariloba複合体(2n=4,8,10,12,16)におけるr-DNAの染色体不の分布．

  ADACHI, WATANABE, FUKUI, OHMIDO, KOSUGA

  日本植物学会 第61回大会研究発表, Sep. 1997, Japanese, 日本植物学会, Narashino, 日本, Domestic conference

  Oral presentation


• Analysis and utility of chromosome information 101. Visualisation of specific tandem repeat DNA (TrsA) and telomere squences on extended DNA fiberes in rice.

  OHMIDO, KIJIMA, HANS, FUKUI

  Exchange Center, Mar. 1997, Japanese, 日本育種学会, Tokyo, 日本, Domestic conference

  Oral presentation


• Analysis of distribution of topoisomerase-II in plant chromosomes.

  FURUMINHAN, OHMIDO, FUKUI, KAMEYA

  日本育種学会 第91回講演会, Mar. 1997, Japanese, 日本育種学会, Tokyo, 日本, Domestic conference

  Oral presentation


• Variation in genome size and chromosome morphology due to repetitive sequences in the genus Oryza.

  UOZU, IKEHASHI, OHMIDO, OHTSUBO, OHTSUBO, FUKUI

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• The mechanism of chromosome structure variation in cultured cells and at first mitoses in germinating aged seeds of maize.

  FLUMINHAN, OHMIDO, FUKUI, PHILLIPS, MIZUGAKI, KAMEYA

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• The distribution of topoisomerase II on maize somatic chromosomes.

  FLUMINHAN, OHMIDO, FUKUI, KAMEYA

  39th annual maize genetics conference, Mar. 1997, English, Florida, アメリカ, International conference

  Poster presentation


• Quantitative chromosome mapping of three diploid genomes of the genus Brassica.

  NAKAYAMA, OHMIDO, FUKUI

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• Phyletic relationship of races and genomic reorganization in the Brachyscome lineariloba Complex.

  WATANABE, ADACHI, FUKUI, OHMIDO, KOSUGE

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• Chromosome analysis of the interspecific hybrids between Nicotiana tabacum L. and Kawakamii Y. Ohashi,using genomic in situ hybridization.

  KITAMURA, NAKAMURA, INOUE, OHMIDO, FUKUI

  日本育種学会 第91回講演会, Mar. 1997, Japanese, 日本育種学会, Tokyo, 日本, Domestic conference

  Oral presentation


• High resolution physical mapping in rice.

  OHMIDO, HANS, FUKUI

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• Heterogenetic association in meiosis of Festuca-Lolium intergeneric F1 hybrids and reciprocal translocation of their F2 generation.

  MIZUKAMI, SUGITA, INABA, OHMIDO, FUKUI

  日本育種学会 第91回講演会, Mar. 1997, Japanese, 日本育種学会, Tokyo, 日本, Domestic conference

  Oral presentation


• Evidence for diploidization of tetraploid burnet (Sanguisorba L.,ROSACEAE).

  MISHIMA, MURATA, IWATSUBO, OHMIDO, FUKUI

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• Distinction of parental chromosomes in somatic hybrids between Solanum integrifolium and S. sanitwongsei using genomic in situ hybridization (GISH).

  IWAMOTO, HIRAI, OHMIDO, FUKUI

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• Direct measurement of 5s ribosomal RNA gene by FISH on extended DNA fibers.

  KIJIMA, OHMIDO, HIROSE, MITUNAGA, OHYAMA, FUKUI

  International Workshop on Analysis and Utility of Plant Chromosome Information, Mar. 1997, English, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, International conference

  Oral presentation


• Quantitative chromosome mapping of three diploid genomes of the genus Brassica.

  NAKAYAMA, OHMIDO, FUKUI

  Plant & Animal Genome V, Jan. 1997, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Poster presentation


• Quantitative analysis of fluorescence in situ hybridization to extended DNA fibers.

  KIJIMA, OHMIDO, HIROSE, MITSUNAGA, OHYAMA, FUKUI

  Plant & Animal Genome V, Jan. 1997, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Poster presentation


• Physical mapping of single copy genes using an improved FISH method in rice

  OHMIDO, FUKUI

  Aberystwhty cell genetics group 7th annual conference, Physical mapping of plant chromosomes, Jan. 1997, English, Aberystwhty cell genetics group, Aberystwyth, イギリス, International conference

  Oral presentation


• Newly propozed basic chromosome number in burnet (Sanguisorba L.,rosaceae): evidence from number of 5S rDNA loci detected by FISH.

  MISHIMA, MURATA, OHMIDO, FUKUI

  Plant & Animal Genome V, Jan. 1997, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Poster presentation


• Immunofluorescence analysis of the distribution of methylated cytosine in maize and rice somatic chromosomes

  FLUMINHAN, OHMIDO, FUKUI, MIZUGAKI, KAMEYA

  Plant & Animal Genome V, Jan. 1997, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Poster presentation


• High resolution physical mapping gene by McFISH in rice.

  OHMIDO, KIJIMA, DRAAISTRA, HANS, FUKUI

  Plant & Animal Genome V, Jan. 1997, English, Scherao International,Inc., SanDiego, アメリカ, International conference

  Poster presentation


• The distribution of 5-methylcytosine in somatic chromosomes of germinating aged rice seeds.

  FLUMINHAN, OHMIDO, FUKUI, KAMEYA

  ISRMB'96 Fifth International Sympsium on Rice Molecular Biology, Oct. 1996, English, ISRMB, Taipei, 台湾, International conference

  Poster presentation


• Visualization of methylated cytosine in maize somatic chromosomes

  FLUMINHAN, OHMIDO, FUKUI, MIZUGAKI, KAMEYA

  The Ministry of Education Science, Sports and Culture, 1996, English, The Ministry of Education Science, Sports and Culture, Sendai, 日本, International conference

  Oral presentation

• 分子生物学会


• 日本遺伝学会


• 日本育種学会


• 染色体学会

• Characterization of two complementary Hydrangea collections

  Nobuko Ohmido

  JSPS, 二国間交流事業共同研究・セミナー, DAAD, Kobe University, Apr. 2020 - Mar. 2022, Principal investigator


• Development of nano-visualization for structural analyses of genetic materials and early infection process for further innovation of functional bio-nanotechnology

  Nobuko Ohmido: Kiichi Fukui, Toshiyuki Wako

  戦略的国際共同研究プログラム（SICORP）, 日本-タイ国・ベトナム国(革新的材料のための機能性バイオナノテクノロジー), Kobe University, Jan. 2018 - Mar. 2022


• Altered gene expression in alien plant chromosome introgression lines

  Nobuko Ohmido

  JSPS, 二国間交流事業共同研究・セミナー, CAS, Apr. 2018 - Mar. 2020, Principal investigator


• Analysis of chromosome inner structure by nucleosome tomography using FIB/SEM

  Fukui Kiichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), 01 Apr. 2013 - 31 Mar. 2018

  Chromosome structure has been an enigma through these three centuries. We tried to elucidate the inner structure of the chromosome by visualization of nucleosome positions by slice and view method using focused ion beam/scanning electron microscopy. As a result, random distribution of nucleosomes were revealed through the chromosome and no regular structure was not detected. On the other hand, we analyzed the chromosome scaffold and revealed that the scaffold consisted with helical double stranded structure. This structure accounts the rigid and flexible nature of the chromosome.


• 六甲山地における防災林機能を高めるエリアマネジメント

  古川 文美子

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2016 - Mar. 2018

  Competitive research funding


• Studies on genomic structure and evolution of spinach sex-determining loci

  Onodera Yasuyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Hokkaido University, 01 Apr. 2014 - 31 Mar. 2017

  Spinach is commonly known as a dioecious species. However, certain lines and crosses produce monoecious individuals. Dioecism and monoecism are of relevance in commercial hybrid seed production, and elucidation of the mechanisms underlying sex determination is valuable for spinach breeding programs. Sex expression in dioecious spinach plants is controlled by a single gene pair termed X and Y. Meiotic recombination was found suppressed in the vicinity of the male-determining locus (Y). A locus controlling the monoecious condition (M) is linked to X/Y locus at 12 cM. In this study, we identified 220 genes linked to X/Y locus, part of which were found to be located in the recombinationally suppressed region. The locus for monoecism was confined to a 25-kbp chromosomal segment.


• Fostering decision-making skills for trans-science problems through knowledge collaborative argumentation

  Sakamoto Miki, ITO MASAYUKI, OUMIDO NOBUKO, HAYASHI HAJIMU

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2014 - 31 Mar. 2017

  This study focuses on three aspects of teaching trans-science problem-solving skills. First, we focused on students’ inquiry skills of science and technology contents. We conducted experimental lessons that aimed to nurture the skills of generating well-defined scientific questions and so on. Second, we elucidated the acquisition process of epistemic understanding from an analysis of learners’ discourse during peer critique. Third, we collected examples of socio-scientific issues-based instruction as well as empirical findings on measurement tasks and evaluation methods from the literature, and attempted to theorize the instruction and evaluation methods systematically. We made a Japanese version of the measurement task and conducted a survey of Japanese college students. Having analyzed the survey results, we determined the characteristics of college students’ decision-making on trans-science problems and elaborated the rubrics.


• 遺伝物質の構造のナノ可視化法の開発によるバイオナノテクノロジーの新たな展開

  近江戸 伸子

  国立研究開発法人科学技術振興機構, 国際科学技術共同研究プログラム(SICORP), 2017, Principal investigator

  Competitive research funding


• アクアフォトミクスによる水を介した生体機能の解明

  ルミアナ ツエンコヴァ

  科学研究費一部基金／基盤研究(B)特設, Apr. 2013 - Mar. 2016

  Competitive research funding


• 欧州における科学技術系博士号取得者のキャリア形成を支援する事業マネジメントの研究

  野上 智行

  科学研究費補助金／基盤研究(B), Apr. 2012 - Mar. 2015

  Competitive research funding


• アクアフォトミクスを用いた生体内水分子構造の解析による細胞の生存能力診断

  ルミアナ ツエンコヴア

  学術研究助成基金助成金／挑戦的萌芽研究, 2011

  Competitive research funding


• ミヤコグサとのシンテニーを利用したダイズのゲノム構造の解析

  原田 久也, 佐藤 修正, 近江戸 伸子

  日本学術振興会, 科学研究費助成事業, 特定領域研究, 独立行政法人農業生物資源研究所, 2008 - 2009

  1. ミスズダイズと秣食豆公503に由来するRILの連鎖地図を基準にして、ミヤコグサの塩基配列情報を利用して比較地図を作製した。F2連鎖地図を用いた結果と同様にダイズの染色体はミヤコグサ連鎖ブロックのモザイクとなっていた。相同染色体は1対ずつで、染色体レベルでは2倍体である。 2. Williams82のゲノム塩基配列から同祖領域を探索したところ、領域の大きさは最大約12.4MBにわたっていることがわかった。同祖領域の分布は極めて複雑でダイズゲノムは複数の倍加と再編成を経て形成されたと考えられる 3. ミヤコグサの花の対称性に関わる遺伝子cyc3のオーソログGmcyc3aを含むBACクローンとGmcyc3aの同祖遺伝子Gmcyc3bを含むBACクローンを異なる蛍光色素でラベルしてパキテン期染色体に同時に貼り付けるとそれぞれ同じ領域にシグナルが観察され、一方のシグナルが強いところは他方のシグナルが弱く見かけ上2つのシグナルがはっきりと認められた。同じことが、同祖遺伝子NFR1a、NFR1bなどでも観察された。また開花期関連遺伝子FT3を含むダイズのTACクローンと相同なミヤコグサのTACクローンは同じ位置にシグナルが見られ強弱が逆転していた。一般にミヤコグサのTACクローンはダイズのBACクローンに比較してシグナルが弱かった。 4. ダイズのゲノムはマメ科植物の中で、極めて特異的であることがわかった。マクロシンテニー、マイクロシンテニーの解析から、倍加と再編成の結果、現在のゲノム構造が生じたと考えられる。ホモロジーの極めて高い同祖遺伝子は、一般に2つ存在していてそれは最も新しい時期の倍加による結果だと考えられる。それらの遺伝子間では機能の分業が生じていて、ダイズは遺伝子レベルでも2倍体としてのシステムを獲得していると言える。


• 他殖性アカクローバー染色体地図のインティグレーション

  近江戸 伸子

  科学研究費補助金／基盤研究(C), 2008, Principal investigator

  Competitive research funding


• 女性科学者育成のためのメンターマインドを養成するワークショップ・プログラムの開発

  朴木 佳緒留

  科学研究費補助金／萌芽研究, 2008

  Competitive research funding


• 社会・国民に支持される科学技術を実現する新しい科学教育システムの構想

  稲垣 成哲

  科学研究費補助金／基盤研究(C), 2006

  Competitive research funding


• 染色体の構造と機能解明のためのナノデバイスに関する総合研究

  近江戸 伸子

  2005, Principal investigator

  Competitive research funding


• マメ科モデル植物ミヤコグサの染色体統合地図の構築

  近江戸 伸子

  科学研究費補助金／基盤研究(C), 2005, Principal investigator

  Competitive research funding


• CSCLシステムを活用した科学教育カリキュラムの日本型モデル

  稲垣 成哲, 大島 純, 村山 功, 近江戸 伸子, 竹中 真希子

  日本学術振興会, 科学研究費助成事業, 特定領域研究, 神戸大学, 2003 - 2004

  本年度の研究実績は,以下であった。 1.文献・資料収集とそのデータベース化 初年度に引き続き,協調的学習に関する理論や方法に関する研究及び情報帳信ネットワークの教育利用に関する研究を収集し,これまでの研究成果や議論を整理した。また,諸外国における研究の先進的な事例を調査するとともに,これら収集した資料をデータベース化し,WWW上で公開した。 2.日本型カリキュラム・モデル試案の検討 初年度に開発した暫定的なCSCLシステム及びカリキュラムのモデルを再検討するとともに,新しく遺伝子工学に関する小学生用の具体的な学習用コンテンツの制作を行った。 3.国内・国際学会等における中間的成果の発表 千葉大学で開催される日本科学教育学会第28回年会において,中間的な発表を行った。また,その他の日本教育工学会等で成果の一部を発表した。さらに,8月にカナダ・トロント大学の応用認知科学センターで開催されるKnowledge Forumの国際会議で発表を行い,北米における同様の研究との比較を試みる中で,本研究で開発したカリキュラムを日本型モデルとして提案した。 4.CSCLを利用した日本型モデルに基づいたカリキュラム開発,運用,評価,公開 3.で報告した学習環境(システム,学習用コンテンツ及びカリキュラム)の日本型モデルの一部を具体化し,神戸大学発達科学部の附属住吉小学校において,1クラス規模の実験授業を行った。また。2004年10月には日本科学教育学会研究会の際に,公開授業研究会を開催し,カリキュラムの普及を行った。本実験授業に即して作成した各種コンテンツ等については,NICERのサーバで広く公開することを予定している。