Research activity information

• Sep. 2024 Ecology and Civil Engineering Society, Hirose Award

  Toshifumi Minamoto


• May 2024 公益社団法人土木学会, 環境賞, 環境DNAを活用した生物モニタリングに向けた調査手法の構築と建設現場への適用

  大成建設(赤塚真依子、高山百合子、伊藤一教、高畑陽、内池智広、渡邉千佳子、大井涼太朗、望月聖、神野桂子、土肥聡、小菅憲正)、神戸大学(源利文)


• Mar. 2023 Kobe City, Kobe SDGs Encouragement Award

  Minamoto Lab at Graduate School of Human Development and Environment, Kobe University


• Jul. 2021 Ecological Society of Japan / Shiga Prefecture, The 21nd Biwako Prize for Ecology

  Toshifumi Minamoto


• Mar. 2019 Murao Educational Foundation, The 36th Academic Award of Murao Educational Foundation

  MINAMOTO Toshifumi

  Publisher


• Dec. 2016 National Institute of Science and Technology Policy, 科学技術への顕著な貢献2016（ナイスステップな研究者）

  MINAMOTO Toshifumi

  Others


• Oct. 2015 Kobe University, President Award of Kobe University

  MINAMOTO Toshifumi

  Others

• Significant improvement of environmental DNA assay by targeting retrotransposon sequences characteristic to Anguilla eels

  Itsuki T. Hirayama, Yuta Kunimasa, Aya Takeuchi, Toshifumi Minamoto

  Last, Sep. 2025, Environmental DNA, (in press), English

  [Refereed]

  Scientific journal


• Hunter, M. E., Sepulveda, A., Trujillo-Gonzalez, A., Helbing, C. C., Rees, H. C., Jones-Slobodian, D. N., Miliano, R. C., Minamoto, T., Theroux, S., Wilcox, T., Yamanaka, H., Villacorta-Rath, C., Klymus, K. E.

  Margaret E. Hunter, Adam Sepulveda, Alejandro Trujillo-Gonzalez, Caren C. Helbing, Helen C. Rees, Devin N. Jones-Slobodian, Rachel C Miliano, Toshifumi Minamoto, Susanna Theroux, Taylor Wilcox, Hiroki Yamanaka, Cecilia Villacorta-Rath, Katy E. Klymus

  Sep. 2025, Environmental DNA, (in press), English

  [Refereed]

  Scientific journal


• Association between objective and subjective relatedness to nature and human well-being: key factors for residents and possible measures for inequality in Japan’s megacities

  Yuta Uchiyama, Akira Kyan, Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto, Kazuhiro Harada, Minoru Takakura, Ryo Kohsaka, Mieko Kiyono, Tetsuya Tsurumi, Atsuhiko Uchida, Tatsuya Saga, Kenta Yamamoto

  Sep. 2025, Landscape and Urban Planning, 261, 105377, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of an eDNA-based qPCR and dPCR method for detecting the spatial distribution and relative abundance of the critically endangered giant barb (Catlocarpio siamensis)

  Jesdakorn Ongkulee, Chatmongkon Suwannapoom, Toshifumi Minamoto, Maslin Osathanunkul

  Elsevier BV, Jun. 2025, Global Ecology and Conservation, 59, e03511, English

  Scientific journal


• Development of universal PCR primers for the environmental DNA metabarcoding of cephalopod (Mollueca) diversity

  Qianqian Wu, Tomoyuki Nakano, So Ishida, Tomoyuki Komai, Yoshihiro Fujiwara, Takao Yoshida, Masaru Kawato, Shin-ichiro Oka, Katsunori Fujikura, Masaki Miya, Toshifumi Minamoto

  Last, Elsevier BV, Apr. 2025, Marine Environmental Research, 208, 107094, English

  [Refereed]

  Scientific journal


• マクロ生物の環境DNA分析：発展の歴史と将来への展望

  源利文

  Apr. 2025, 応用生態工学, (in press), Japanese

  [Refereed][Invited]

  Scientific journal


• Seven-year changes in eDNA concentrations of two dominant submerged macrophytes in Lake Shinji: effects of salinity

  Teruhiko Takahara, Satoshi Yamagishi, Rina Shimoda, Akihiro Nagata, Masayuki K. Sakata, Hideyuki Doi, Toshifumi Minamoto

  Elsevier BV, Apr. 2025, Estuarine, Coastal and Shelf Science, 315, 109165, English

  [Refereed]

  Scientific journal


• Reconstruction of marimo population dynamics over 200 years using molecular markers and fossil plankton remains

  Jotaro Urabe, Isamu Wakana, Hajime Ohtsuki, Masayuki K. Sakata, Yurie Ohtake, Ryotaro Ichige, Michinobu Kuwae, Toshifumi Minamoto

  Mar. 2025, Environmental DNA, 7, e70085, English

  [Refereed]

  Scientific journal


• Influence of homogenization methods on lichen species detection from environmental DNA metabarcoding

  Ayumi Sakata, Toshifumi Minamoto, Tetsuya Sado, Ryo O. Gotoh, Masaki Miya

  Mar. 2025, Metabarcoding and Metagenomics, 9, e144340, English

  [Refereed]

  Scientific journal


• Improved environmental DNA detection sensitivity of Opisthorchis viverrini using a multi-marker assay

  Riko Matsuo, Ayana Togetani, Poom Adisakwattana, Tippayarat Yoonuan, Orawan Phuphisu, Yanin Limpanon, Masayuki K. Sakata, Marcello Otake Sato, Megumi Sato, Toshifumi Minamoto

  Dec. 2024, Parasitology Research, 123, 419, English

  [Refereed]

  Scientific journal


• Methodological assessment for efficient collection of Schistosoma mansoni environmental DNA and improved schistosomiasis surveillance in tropical wetlands

  Ryosuke Osawa, Toshiaki S. Jo, Risa Nakamura, Kyoko Futami, Tomoaki Itayama, Evans Asena Chadeka, Benard Ngetich, Sachiyo Nagi, Mihoko Kikuchi, Sammy M. Njenga, Collins Ouma, George O. Sonye, Shinjiro Hamano, Toshifumi Minamoto

  Elsevier BV, Dec. 2024, Acta Tropica, 260, 107402, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Local environment perceived in daily life and urban green and blue space visits: Uncovering key factors for different age groups to access ecosystem services

  Yuta Uchiyama, Akira Kyan, Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto, Mieko Kiyono, Kazuhiro Harada, Minoru Takakura

  Nov. 2024, Journal of Environmental Management, 370, 122676, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Stability of environmental DNA methylation and its utility in tracing spawning in fish

  Itsuki T. Hirayama, Luhan Ưu, Toshifumi Minamoto

  Last, Oct. 2024, Molecular Ecology Resources, 24(8) (8), e14011, English

  [Refereed]

  Scientific journal


• Application of dietary content analysis using dropped feces to assess damage to fisheries by great cormorants

  Tetsuo Asai, Shiori Ikushima, Michiyo Sugiyama, Tomoya Morimoto, Akiko Sudo, Toshifumi Minamoto

  Ornithological Society of Japan, Sep. 2024, Ornithological Science, 23(2) (2), 125 - 128, English

  [Refereed]

  Scientific journal


• Detection of environmental DNA of finless porpoise (Neophocaena asiaeorientalis) in Osaka Bay, Japan

  Nagisa Hashimoto, Takashi Iwata, Natsumi Kihara, Kiyomi Nakamura, Masayuki K. Sakata, Toshifumi Minamoto

  Sep. 2024, Conservation Genetics Resources, 16, 255 - 261, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of specific detection assays from environmental DNA of invasive diatom Cymbella janischii in Japan

  Yoko Kato-Unoki, Shigeki Mayama, Akira Kurihara, Toshifumi Minamoto

  Springer Science and Business Media LLC, Aug. 2024, Limnology, 25(3) (3), 247 - 254, English

  [Refereed]

  Scientific journal


• Traces of pandemic fluoroquinolone-resistant Escherichia coli clone ST131 transmitted from human society to aquatic environments and wildlife in Japan

  Toyotaka Sato, Kojiro Uemura, Mitsuru Yasuda, Aiko Maeda, Toshifumi Minamoto, Kazuki Harada, Michiyo Sugiyama, Shiori Ikushima, Shin-ichi Yokota, Motohiro Horiuchi, Satoshi Takahashi, Testuo Asai

  Elsevier BV, Jun. 2024, One Health, 18, 100715, English

  [Refereed]

  Scientific journal


• Sensitive and efficient surveillance of Japanese giant salamander (Andrias japonicus) distribution in western Japan using multi-copy nuclear DNA marker

  Shunsuke Hidaka, Toshiaki S. Jo, Satoshi Yamamoto, Koki R. Katsuhara, Sei Tomita, Masaki Miya, Makihiko Ikegami, Atushi Ushimaru, Toshifumi Minamoto

  Abstract Japanese giant salamander (Andrias japonicus) is one of the largest amphibian species in the world and an iconic species in Japan. However, as its distribution has recently declined across the country, rapid and extensive monitoring of the distribution is urgently needed for its efficient conservation. Here, we used environmental DNA (eDNA) analysis to assess the Japanese giant salamander’s distribution in western Japan and, for that purpose, we collected 410 water samples from 12 rivers. We then developed a new eDNA assay for multi-copy nuclear DNA (nuDNA) of the giant salamander and compared the eDNA detectability of the nuDNA marker with that of a previous mitochondrial DNA (mtDNA) marker. Throughout the survey, we detected target eDNA from 162 water samples using either of the markers, which generally corresponded to the known natural distribution of the species. Additionally, the use of the nuDNA marker allowed for higher detection rate of target eDNA than the mtDNA marker. Moreover, the detection rate of target eDNA decreased substantially in water samples with higher conductivity and also partly in those with higher pH, suggesting their negative impacts on the salamander’s ecology. Our results demonstrated that eDNA analysis with multi-copy nuDNA marker is highly useful for efficient and sensitive surveillance of Japanese giant salamander’s distribution. Our study provided the methodology for efficiently monitoring the Japanese giant salamander’s distribution via eDNA analysis and facilitating conservation activities for them.

  Springer Science and Business Media LLC, Apr. 2024, Limnology, 25(2) (2), 189 - 198, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The effect of artificial light at night on wild fish community: manipulative field experiment and species composition analysis using environmental DNA

  Aisha Oyabu, Luhan Wu, Takehiro Matsumoto, Natsumi Kihara, Hiroki Yamanaka, Toshifumi Minamoto

  Last, Elsevier BV, Apr. 2024, Environmental Advances, 15, 100457, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Monitoring of multiple fish species by quantitative environmental DNA metabarcoding surveys over two summer seasons

  Luhan Wu, Tomonori Osugi, Takashi Inagawa, Jiro Okitsu, Shogo Sakamoto, Toshifumi Minamoto

  Abstract Periodic monitoring can provide important information for the protection of endangered fish, sustainable use of fishery resources and management of alien species. Previous studies have attempted to monitor fish using non‐invasive environmental DNA (eDNA) technology, generally employing quantitative PCR to quantify the eDNA concentration. However, the throughput was limited. High‐throughput metabarcoding technology can detect the DNA of multiple species simultaneously in a single experiment but does not provide sufficient quantification. In this study, we applied a quantitative metabarcoding approach to simultaneously quantify the eDNA concentration of an entire fish assemblage in a small reservoir over two summer seasons. Traditional surveys were also conducted to investigate the individuals of fish. The eDNA concentrations were quantified using quantitative metabarcoding, and the fish species detected using this approach were highly consistent with the results of traditional fish monitoring. A significant positive relationship was observed between the eDNA concentration and fish species abundance. Seasonal changes in fish community structure were estimated using eDNA concentrations, which may reveal the activity seasons of different fish. The eDNA concentrations of different fish species peaked at different water temperatures, reflecting the differential responses of fish species to this environmental factor. Finally, by detecting outlier eDNA concentrations, the spawning activities of 13 fish species were estimated, 12 of which were roughly consistent with the current knowledge of fish spawning periods. These results indicate that quantitative eDNA metabarcoding with dozens of sampling times is useful for the simultaneous ecological monitoring of multiple fish species.

  Last, Wiley, Jan. 2024, Molecular Ecology Resources, 24, e13875, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan

  Hiroki Hashizume, Suguru Taga, Masayuki K. Sakata, Mahmoud Hussein, Emmanuel Edwar Siddig, Toshifumi Minamoto, Ahmed Hassan Fahal, Satoshi Kaneko

  Dec. 2023, Tropical Medicine and Health, 51, 71, English

  [Refereed]

  Scientific journal


• A study on the distribution characteristics of environmental DNA associated with seagrass beds and the sampling area selection procedure

  Maiko AKATSUKA, Kotaro IIMURA, Yuriko TAKAYAMA, Toshifumi MINAMOTO

  Last, Japan Society of Civil Engineers, Nov. 2023, Japanese Journal of JSCE, 79(17) (17), 23-27158, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Regional-scale effects of deer-induced forest degradation on river ecosystem dynamics

  Hikaru Nakagawa, Daisuke Fujiki, Hiroo Numata, Luhan Wu, Terutaka Mori, Toshifumi Minamoto

  Last, Nov. 2023, Population Ecology, (in press), English

  [Refereed]

  Scientific journal


• Comparison of evolutionary relationships between Branchiostoma floridae, Ciona intestinalis, and Homo sapiens globins provide evidence of gene cooption and convergent evolution

  Nanako Yano, Toshifumi Minamoto, Hiroshi Yamaguchi, Toshiyuki Goto, Takahito Nishikata

  Nov. 2023, International Journal of Molecular Sciences, 24(21) (21), 16009, English

  [Refereed]

  Scientific journal


• Effective environmental DNA collection for an endangered catfish species: testing for habitat and daily periodicity

  Teruhiko Takahara, Hideyuki Doi, Toshihiro Kosuge, Nanae Nomura, Nobutaka Maki, Toshifumi Minamoto, Katsutoshi Watanabe

  Springer Science and Business Media LLC, Nov. 2023, Ichthyological Research, 70(4) (4), 409 - 418, English

  [Refereed]

  Scientific journal


• Opposite trends in environmental DNA distributions of two freshwater species under climate change

  Qianqian Wu, Jinxin Zhou, Tatsuya Komoto, Toshiyuki Ishikawa, Naoshige Goto, Masayuki K. Sakata, Daisuke Kitazawa, Toshifumi Minamoto

  Last, Sep. 2023, Ecosphere, 14(9) (9), e4651, English

  [Refereed]

  Scientific journal


• Spatial epidemiology model can explain the seasonal dynamics of infectious disease Cyprinid herpesvirus 3 (CyHV-3) by thermoregulation behavior of the host, common carp (Cyprinus carpio)

  Takeshi Miki, Hiroki Yamanaka, Atsushi Sogabe, Koji Omori, Yasuhisa Saito, Toshifumi Minamoto, Kimiko Uchii, Mie N. Honjo, Alata A. Suzuki, Yukihiro Kohmatsu, Zen’ichiro Kawabata

  Springer Science and Business Media LLC, Sep. 2023, Theoretical Ecology, 16(3) (3), 195 - 208, English

  [Refereed]

  Scientific journal


• An interdisciplinary approach to environmental conservation policy: a case of Satoyama redevelopment in the peri-urban area

  Masayuki Sato, Toshifumi Minamoto, Atushi Ushimaru

  Abstract This study proposes a practice and discussion for an interdisciplinary approach to policies for the conservation of suburban and peri-urban ecosystems. We highlight the need for evidence-based assessment of the current quality of the targeted nature from perspectives of natural science and problem formulation, and that causes should be investigated from the combined perspectives of social science, economic evaluation, and policy design and evaluation, with an awareness of the possibility of consensus building. In this study, based on the ongoing international trend of ecosystem conservation, an economic analysis was conducted to examine the direction of Satoyama development as a case study of urban and peri-urban ecosystem conservation. The result identified the preference and needs of citizens with regard to Satoyama ecosystems and discussed the consistency between policy targets and citizens’ evaluation.

  Springer Science and Business Media LLC, Jul. 2023, International Journal of Economic Policy Studies, 17(2) (2), 403 - 419, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Species traits and ecosystem characteristics affect species detection by eDNA metabarcoding in lake fish communities

  Hideyuki Doi, Shunsuke Matsuoka, Shin‐ichiro S. Matsuzaki, Mariko Nagano, Hirotoshi Sato, Hiroki Yamanaka, Saeko Matsuhashi, Satoshi Yamamoto, Toshifumi Minamoto, Hitoshi Araki, Kousuke Ikeda, Atsuko Kato, Kouichi Kumei, Nobutaka Maki, Takashi Mitsuzuka, Teruhiko Takahara, Kimihito Toki, Natsuki Ueda, Takeshi Watanabe, Kanji Yamazoe, Masaki Miya

  Abstract Although environmental DNA (eDNA) metabarcoding is an exceptionally useful and powerful tool for monitoring biodiversity, little is known about whether the traits of organisms and their ecosystem characteristics affect eDNA metabarcoding performance. Nationwide surveys can provide more detailed insights, yet such studies have rarely been conducted. In order to evaluate eDNA metabarcoding performance in broad‐scale monitoring, we examined the effects of species ecological/biological traits and ecosystem characteristics on species detection rates and the implications for community analysis. In addition, we tested the effects of sample mixing and transportation methods, including cooling and freezing, on eDNA metabarcoding. On a nationwide scale, we conducted eDNA metabarcoding for fish communities in 18 Japanese lakes. By comparing species records, we observed that certain traits, including body size, body shape, saltwater tolerance and habitat preference, influenced eDNA detection. In addition, the proportion of species detected decreased significantly with an increase in lake surface area owing to ecosystem size effect on species detection. We conclude that species traits, including habitat preference, body size and ecosystem size, should be considered when assessing the eDNA metabarcoding performance in broad‐scale monitoring.

  Wiley, Jul. 2023, Freshwater Biology, 68(8) (8), 1346 - 1358, English

  [Refereed]

  Scientific journal


• Detection of Echinococcus multilocularis in repurposed environmental DNA samples from river water

  Kensuke Mori, Akio Imamura, Itsuki Hirayama, Toshifumi Minamoto

  Last, Jun. 2023, PeerJ, 11, e15431, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparing environmental DNA with whole pond survey to estimate the total biomass of fish species in ponds

  Rio Souma, Izumi Katano, Hideyuki Doi, Teruhiko Takahara, Toshifumi Minamoto

  Wiley, May 2023, Freshwater Biology, 68(5) (5), 727 - 736, English

  [Refereed]

  Scientific journal


• Heterogeneous preference for biodiversity in Japanese urban blue spaces based on people's nature experiences: Analysis using eDNA and satisfaction data

  Ippei Aoshima, Ryohei Nakao, Toshifumi Minamoto, Atushi Ushimaru, Masayuki Sato

  Elsevier BV, Apr. 2023, City and Environment Interactions, 18, 100101, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• An efficient environmental DNA detection method for rare species: a case study of a small salamander (Hynobius boulengeri)

  Masayuki K. Sakata, Daiki Takeshita, Ryohei Nishizawa, Takuya Sato, Toshifumi Minamoto

  Springer Science and Business Media LLC, Mar. 2023, Analytical Sciences, 39(5) (5), 721 - 728, English

  [Refereed]

  Scientific journal


• Development of two new sets of PCR primers for eDNA metabarcoding of brittle stars (Echinodermata, Ophiuroidea)

  Masanori Okanishi, Hisanori Kohtsuka, Qianqian Wu, Junpei Shinji, Naoki Shibata, Takashi Tamada, Tomoyuki Nakano, Toshifumi Minamoto

  Brittle stars (class Ophiuroidea) are marine invertebrates comprising approximately 2,100 extant species, and are considered to constitute the most diverse taxon of the phylum Echinodermata. As a non-invasive method for monitoring biodiversity, we developed two new sets of PCR primers for metabarcoding environmental DNA (eDNA) from brittle stars. The new primer sets were designed to amplify 2 short regions of the mitochondrial 16S rRNA gene, comprising a conserved region (111–115 bp, 112 bp on average; named “16SOph1”) and a hyper-variable region (180–195 bp, 185 bp on average; named “16SOph2”) displaying interspecific variation. The performance of the primers was tested using eDNA obtained from two sources: a) rearing water of an 2.5 or 170 L aquarium tanks containing 15 brittle star species and b) from natural seawater collected around Misaki, the Pacific coast of central Japan, at depths ranging from shallow (2 m) to deep (> 200 m) sea. To build a reference library, we obtained 16S rRNA sequences of brittle star specimens collected from around Misaki and from similar depths in Japan, and sequences registered in International Nucleotide Sequence Database Collaboration. As a result of comparison of the obtained eDNA sequences with the reference library 37 (including cryptic species) and 26 brittle star species were detected with certain identities by 16SOph1 and 16SOph2 analyses, respectively. In shallow water, the number of species and reads other than the brittle stars detected with 16SOph1 was less than 10% of the total number. On the other hand, the number of brittle star species and reads detected with 16SOph2 was less than half of the total number, and the number of detected non-brittle star metazoan species ranged from 20 to 46 species across 6 to 8 phyla (only the reads at the “Tank” were less than 0.001%). The number of non-brittle star species and reads at 80 m was less than 10% with both of the primer sets. These findings suggest that 16SOph1 is specific to the brittle star and 16SOph2 is suitable for a variety of marine metazoans. It appears, however, that further optimization of primer sequences would still be necessary to avoid possible PCR dropouts from eDNA extracts. Moreover, a detailed elucidation of the brittle star fauna in the examined area, and the accurate identification of brittle star species in the current DNA databank is required.

  Pensoft Publishers, Feb. 2023, Metabarcoding and Metagenomics, 7, e94298, English

  [Refereed]

  Scientific journal


• Improvement of recovery yield of macro-organismal environmental DNA from seawater samples

  Qianqian Wu, Toshifumi Minamoto

  Abstract In recent years, environmental DNA (eDNA) technology has been used in a variety of water environments. Environmental DNA concentrations in marine samples tend to be lower than those in freshwater samples, and few studies have explored methods to improve the recovery yields of eDNA from seawater samples. In this study, we compared different seawater preservation solutions (RNAlater or ATL) to improve eDNA yields. The eDNA concentrations of vertebrate and invertebrate species were compared using species-specific eDNA assays, and the number of detected fish and their compositions were compared using metabarcoding analysis. ATL treatment resulted in significantly higher eDNA yields for both vertebrate and invertebrate species than RNAlater treatment. Metabarcoding analysis revealed non-significant effects of preservation on the number of detected species and species composition. These results suggest that ATL treatment improves DNA yields without changing the species composition compared with the commonly used RNAlater treatment. The findings of this study will reduce false-negative outcomes and provide highly reliable results in future biological surveys. Graphical abstract

  Last, Springer Science and Business Media LLC, Feb. 2023, Analytical Sciences, 39(5) (5), 713 - 720, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimating the spawning activity of fish species using nuclear and mitochondrial environmental DNA concentrations and their ratios

  Luhan Wu, Qianqian Wu, Takashi Inagawa, Jiro Okitsu, Shogo Sakamoto, Toshifumi Minamoto

  Last, Wiley, Jan. 2023, Freshwater Biology, 68(1) (1), 103 - 114, English

  [Refereed]

  Scientific journal


• Development of primer–probe sets for environmental DNA-based monitoring of pond smelt Hypomesus nipponensis and Japanese icefish Salangichthys microdon

  Teruhiko Takahara, Katsuya Fukui, Daisuke Hiramatsu, Hideyuki Doi, Masato Fujii, Toshifumi Minamoto

  Springer Science and Business Media LLC, Jan. 2023, Landscape and Ecological Engineering, 19(1) (1), 11 - 19, English

  [Refereed]

  Scientific journal


• Development of environmental DNA detection assays for snakes in paddy fields in Japan

  Ryohei Nishizawa, Ryohei Nakao, Atushi Ushimaru, Toshifumi Minamoto

  Springer Science and Business Media LLC, Jan. 2023, Landscape and Ecological Engineering, 19(1) (1), 3 - 10, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Influence of flow in seagrass bed monitoring utilizing environmental ANA analysis

  Maiko Akatsuka, Edwin Muchebve, Yuriko Takayama, Yukinobu Oda, Toshifumi Minamoto

  Last, Nov. 2022, J. JSCE, Ser. B2, Coastal Engineering, 78(2) (2), I_865 - I_870, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Relationships between the eDNA concentration obtained from metabarcoding and stream fish abundance estimated by the removal method under field conditions

  Hikaru Nakagawa, Keitaro Fukushima, Masaru Sakai, Luhan Wu, Toshifumi Minamoto

  Wiley, Nov. 2022, Environmental DNA, 4(6) (6), 1369 - 1380, Japanese

  [Refereed]

  Scientific journal


• Valuing the cultural services from urban blue‐space ecosystems in Japanese megacities during the COVID‐19 pandemic

  Toshiaki Jo, Masayuki Sato, Toshifumi Minamoto, Atushi Ushimaru

  Wiley, Oct. 2022, People and Nature, 4(5) (5), 1176 - 1189, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Isolation of extended-spectrum beta-lactamase-producing Escherichia coli from Japanese red fox (Vulpes vulpes japonica)

  Tetsuo Asai, Michiyo Sugiyama, Tsutomu Omatsu, Masato Yoshikawa, Toshifumi Minamoto

  Sep. 2022, MicrobiologyOpen, 11(5) (5), e1317, English

  [Refereed]

  Scientific journal


• Spatiotemporal changes in environmental DNA concentrations caused by fish spawning activity

  Luhan Wu, Yoshihiko Yamamoto, Shogo Yamaguchi, Toshifumi Minamoto

  Last, Elsevier BV, Sep. 2022, Ecological Indicators, 142, 109213 - 109213, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens

  Masayuki K. Sakata, Megumi Sato, Marcello Otake Sato, Tomoe Watanabe, Honami Mitsuishi, Tomoyuki Hikitsuchi, Jun Kobayashi, Toshifumi Minamoto

  Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics related to the persistence of immature mosquito eDNA. We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. In the future, monitoring immature mosquitoes using eDNA analysis will contribute to prevent mosquito-borne infectious diseases.

  Last, Public Library of Science (PLoS), Aug. 2022, PLOS ONE, 17(8) (8), e0272653 - e0272653, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Applicability of environmental DNA surveys in agricultural waterways and diffusion distances of environmental DNA

  Noriyuki Koizumi, Toshifumi Minamoto, Tomoyasu Shirako, Masatoshi Nakamura

  Aug. 2022, Water, Land and Environmental Engineering, 90(8) (8), 11 - 14, Japanese

  [Refereed][Invited]

  Scientific journal


• Slower growth of farmed eels stocked into rivers with higher wild eel density

  Ryoshiro Wakiya, Hikaru Itakura, Tatsumu Hirae, Tadamitsu Igari, Miyuki Manabe, Noriaki Matsuya, Katsushi Miyata, Masayuki K. Sakata, Toshifumi Minamoto, Takashi Yada, Kenzo Kaifu

  Wiley, Jun. 2022, Journal of Fish Biology, 101(3) (3), 613 - 627, English

  [Refereed]

  Scientific journal


• Environmental DNA analysis for macro-organisms: Species distribution and more

  Toshifumi Minamoto

  Abstract In an era of severe biodiversity loss, biological monitoring is becoming increasingly essential. The analysis of environmental DNA (eDNA) has emerged as a new approach that could revolutionize the biological monitoring of aquatic ecosystems. Over the past decade, macro-organismal eDNA analysis has undergone significant developments and is rapidly becoming established as the golden standard for non-destructive and non-invasive biological monitoring. In this review, I summarize the development of macro-organismal eDNA analysis to date and the techniques used in this field. I also discuss the future perspective of these analytical methods in combination with sophisticated analytical techniques for DNA research developed in the fields of molecular biology and molecular genetics, including genomics, epigenomics, and single-cell technologies. eDNA analysis, which to date has been used primarily for determining the distribution of organisms, is expected to develop into a tool for elucidating the physiological state and behavior of organisms. The fusion of microbiology and macrobiology through an amalgamation of these technologies is anticipated to lead to the future development of an integrated biology.

  Oxford University Press (OUP), Jun. 2022, DNA Research, 29(3) (3), dsac018, English

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan

  Hiroki Hashizume, Suguru Taga, Masayuki K. Sakata, Mahmoud Hussein Mohamed Taha, Emmanuel Edwar Siddig, Toshifumi Minamoto, Ahmed Hassan Fahal, Satoshi Kaneko

  Mycetoma is a tropical disease caused by several fungi and bacteria present in the soil. Fungal mycetoma and eumycetoma are especially challenging to treat; therefore, prevention, early diagnosis, and early treatment are important, but it is also necessary to understand the geographic distribution of these pathogenic fungi. In this study, we used DNA metabarcoding methodology to identify fungal species from soil samples. Soil sampling was implemented at seven villages in an endemic area of Sennar State in Sudan in 2019, and ten sampling sites were selected in each village according to land-use conditions. In total, 70 soil samples were collected from ground surfaces, and DNA in the soil was extracted with a combined method of alkaline DNA extraction and a commercial soil DNA extraction kit. The region for universal primers was selected to be the ribosomal internal transcribed spacer one region for metabarcoding. After the second PCR for DNA library preparation, the amplicon-based DNA analysis was performed using next-generation sequencing with two sets of universal primers. A total of twelve mycetoma-causative fungal species were identified, including the prime agent, Madurella mycetomatis, and additional pathogens, Falciformispora senegalensis and Falciformispora tompkinsii, in 53 soil samples. This study demonstrated that soil DNA metabarcoding can elucidate the presence of multiple mycetoma-causative fungi, which may contribute to accurate diagnosis for patient treatment and geographical mapping.

  Public Library of Science (PLoS), Mar. 2022, PLoS Neglected Tropical Diseases, 16(3) (3), e0010274 - e0010274, English

  [Refereed]

  Scientific journal


• Linking the state of environmental DNA to its application for biomonitoring and stock assessment: Targeting mitochondrial/nuclear genes, and different DNA fragment lengths and particle sizes

  Toshiaki Jo, Kenta Takao, Toshifumi Minamoto

  Last, Wiley, Mar. 2022, Environmental DNA, 4(2) (2), 271 - 283, English

  [Refereed][Invited]

  Scientific journal


• Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia

  Masayuki K. Sakata, Mone U. Kawata, Atsushi Kurabayashi, Takaki Kurita, Masatoshi Nakamura, Tomoyasu Shirako, Ryosuke Kakehashi, Kanto Nishikawa, Mohamad Yazid Hossman, Takashi Nishijima, Junichi Kabamoto, Masaki Miya, Toshifumi Minamoto

  Biodiversity monitoring is important for the conservation of natural ecosystems in general, but particularly for amphibians, whose populations are pronouncedly declining. However, amphibians’ ecological traits (e.g. nocturnal or aquatic) often prevent their precise monitoring. Environmental DNA (eDNA) metabarcoding – analysis of extra-organismal DNA released into the environment – allows the easy and effective monitoring of the biodiversity of aquatic organisms. Here, we developed and tested the utility of original PCR primer sets. First, we conducted in vitro PCR amplification tests with universal primer candidates using total DNA extracted from amphibian tissues. Five primer sets successfully amplified the target DNA fragments (partial 16S rRNA gene fragments of 160–311 bp) from all 16 taxa tested (from the three living amphibian orders Anura, Caudata and Gymnophiona). Next, we investigated the taxonomic resolution retrieved using each primer set. The results revealed that the universal primer set “Amph16S” had the highest resolution amongst the tested sets. Finally, we applied Amph16S to the water samples collected in the field and evaluated its detection capability by comparing the species detected using eDNA and physical survey (capture-based sampling and visual survey) in multiple agricultural ecosystems across Japan (160 sites in 10 areas). The eDNA metabarcoding with Amph16S detected twice as many species as the physical surveys (16 vs. 8 species, respectively), indicating the effectiveness of Amph16S in biodiversity monitoring and ecological research for amphibian communities.

  Last, Pensoft Publishers, Feb. 2022, Metabarcoding and Metagenomics, 6, 15 - 26, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Autumn dispersal and limited success of reproduction of the deepbody bitterling (Acheilognathus longipinnis) in terrestrialized floodplain

  Shigeya Nagayama, Munehiro Oota, Tomohiko Fujita, Jyun-ichi Kitamura, Toshifumi Minamoto, Seiichi Mori, Masayuki Kato, Naofumi Takeyama, Fumiya Takino, Ryuji Yonekura, Hiroki Yamanaka

  Feb. 2022, Knowledge and Management of Aquatic Ecosystems, 423, 4, English

  [Refereed]

  Scientific journal


• Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA

  Sachia Sasano, Hiroaki Murakami, Keita W. Suzuki, Toshifumi Minamoto, Yoh Yamashita, Reiji Masuda

  Springer Science and Business Media LLC, Jan. 2022, Fisheries Science, 88(1) (1), 91 - 107, English

  [Refereed]

  Scientific journal


• Environmental DNA emission by two carangid fishes in single and mixed-species tanks

  Hiroaki Murakami, Reiji Masuda, Satoshi Yamamoto, Toshifumi Minamoto, Yoh Yamashita

  Springer Science and Business Media LLC, Jan. 2022, Fisheries Science, 88(1) (1), 55 - 62, English

  [Refereed]

  Scientific journal


• Pre-centrifugation before DNA extraction mitigates extraction efficiency reduction of environmental DNA caused by the preservative solution (benzalkonium chloride) remaining in the filters

  Satsuki Tsuji, Ryohei Nakao, Minoru Saito, Toshifumi Minamoto, Yoshihisa Akamatsu

  Springer Science and Business Media LLC, Jan. 2022, Limnology, 23(1) (1), 9 - 16, English

  [Refereed]

  Scientific journal


• Universal performance of benzalkonium chloride for the preservation of environmental DNA in seawater samples

  Toshiaki Jo, Masayuki K. Sakata, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

  Last, Wiley, Nov. 2021, Limnology and Oceanography: Methods, 19(11) (11), 758 - 768, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Study on an environmental DNA analysis protocol for seagrass bed monitoring

  Maiko AKATSUKA, Yuriko TAKAYAMA, Edwin MUCHEBVE, Kazunori ITO, Kenta WATANABE, Tomohiro KUWAE, Toshifumi MINAMOTO

  環境DNAを活用した藻場の増減を捉えるモニタリング方法の開発には，採水，分析手順のプロトコル化が重要である．これまでの研究で，現地調査での海草の環境DNA量は，1Lの海水を用いる従来法で定量下限以下となり，ろ過量を増やし，環境DNAを多く回収することで定量分析が可能になることを確認してきた．しかしながら，現地調査で想定される懸濁物質等により必要量をろ過できない場合の対策法は確立されていない．本研究では，低濃度で懸濁物質が多い試料から海草のDNAを多く回収する方法を検討し，現地調査に適応可能な分析方法の有効性を確認した．また，コアマモ場の入り江を対象に数値計算を実施し，採水に適した時間帯が地点毎に異なり，現地調査に向けて採水地点，時刻選定が重要であることを示した．

  Japan Society of Civil Engineers, Nov. 2021, Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering), 77(2) (2), I_895 - I_900, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Discovery of residual Ayu fish in the backwater area of the Yodo River Barrage

  Yuichi Seguchi, Yoshihiko Yamamoto, Yasuhiro Takemon, Toshifumi Minamoto

  Nov. 2021, Japanese Journal of Ichthyology, 68(2) (2), 163 - 172, Japanese

  [Refereed]

  Scientific journal


• Prevalence of Antimicrobial-Resistant Escherichia coli in Migratory Greater White-Fronted Geese (Anser albifrons) and their Habitat in Miyajimanuma, Japan

  Akira Fukuda, Masaru Usui, Katsumi Ushiyama, Dipti Shrestha, Nagisa Hashimoto, Masayuki K. Sakata, Toshifumi Minamoto, Osamu Yoshida, Kanako Murakami, Yutaka Tamura, Tetsuo Asai

  The spread of antimicrobial-resistant bacteria (ARB) in natural environments including wild animals is a concern for public health. Birds cover large areas, and some fly across borders to migrate in large flocks. As a migratory bird, the Greater White-fronted Goose (Anser albifrons) travels to Miyajimanuma, North Japan, each spring and autumn. To investigate the ARB in migratory birds and their surroundings, we collected 110 fecal samples of A. albifrons and 18 water samples from Miyajimanuma in spring and autumn of 2019. Isolation of Escherichia coli was performed using selective agars with or without antimicrobials (cefazolin and nalidixic acid). Isolates of E. coli were recovered from 56 fecal samples (50.9%) and five water samples (27.8%) on agars without antimicrobials. No isolates were recovered on agars with antimicrobials. One E. coli isolate derived from a fecal sample exhibited resistance to β-lactams (ampicillin and cefazolin), whereas all other isolates exhibited susceptibility to all tested antimicrobials. The resistant isolate harbored blaACC, which could be transferred to other bacteria and confer resistance to β-lactams. These results suggest a low prevalence of antimicrobial resistance in wild migratory birds and their living environments; however, wild migratory birds sometimes carry ARB harboring transferrable antimicrobial resistance genes and therefore present a risk of spreading antimicrobial resistance.

  Wildlife Disease Association, Oct. 2021, Journal of Wildlife Diseases, 57(4) (4), 954 - 958, English, International magazine

  [Refereed]

  Scientific journal


• eDNA‐based detection of a vulnerable crocodile newt (Tylototriton uyenoi) to influence government policy and raise public awareness

  Maslin Osathanunkul, Toshifumi Minamoto

  Wiley, Oct. 2021, Diversity and Distributions, 27(10) (10), 1958 - 1965, English

  [Refereed]

  Scientific journal


• Environmental DNA preserved in marine sediment for detecting jellyfish blooms after a tsunami

  Mizuki Ogata, Reiji Masuda, Hiroya Harino, Masayuki K. Sakata, Makoto Hatakeyama, Katsuhide Yokoyama, Yoh Yamashita, Toshifumi Minamoto

  Environmental DNA (eDNA) can be a powerful tool for detecting the distribution and abundance of target species. This study aimed to test the longevity of eDNA in marine sediment through a tank experiment and to use this information to reconstruct past faunal occurrence. In the tank experiment, juvenile jack mackerel (Trachurus japonicus) were kept in flow-through tanks with marine sediment for two weeks. Water and sediment samples from the tanks were collected after the removal of fish. In the field trial, sediment cores were collected in Moune Bay, northeast Japan, where unusual blooms of jellyfish (Aurelia sp.) occurred after a tsunami. The samples were analyzed by layers to detect the eDNA of jellyfish. The tank experiment revealed that after fish were removed, eDNA was not present in the water the next day, or subsequently, whereas eDNA was detectable in the sediment for 12 months. In the sediment core samples, jellyfish eDNA was detected at high concentrations above the layer with the highest content of polycyclic aromatic hydrocarbons, reflecting tsunami-induced oil spills. Thus, marine sediment eDNA preserves a record of target species for at least one year and can be used to reconstruct past faunal occurrence.

  Springer Science and Business Media LLC, Aug. 2021, Scientific Reports, 11(1) (1), 16830, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Application of environmental DNA metabarcoding in a lake with extensive algal blooms

  Qianqian Wu, Masayuki K. Sakata, Deyi Wu, Hiroki Yamanaka, Toshifumi Minamoto

  Springer Science and Business Media LLC, Aug. 2021, Limnology, 22(3) (3), 363 - 370, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Complex interactions between environmental DNA (eDNA) state and water chemistries on eDNA persistence suggested by meta-analyses.

  Toshiaki Jo, Toshifumi Minamoto

  Understanding the processes of environmental DNA (eDNA) persistence and degradation is essential to determine the spatiotemporal scale of eDNA signals and accurately estimate species distribution. The effects of environmental factors on eDNA persistence have previously been examined; however, the influence of the physiochemical and molecular states of eDNA on its persistence is not completely understood. Here, we performed meta-analyses including 26 previously published papers on the estimation of first-order eDNA decay rate constants, and assessed the effects of filter pore size, DNA fragment size, target gene, and environmental conditions on eDNA decay rates. Almost all supported models included the interactions between the filter pore size and water temperature, between the target gene and water temperature, and between the target gene and water source, implying the influence of complex interactions between the eDNA state and environmental conditions on eDNA persistence. These findings were generally consistent with the results of a re-analysis of a previous tank experiment which measured the time-series changes in marine fish eDNA concentrations in multiple size fractions after fish removal. Our results suggest that the mechanism of eDNA persistence and degradation cannot be fully understood without knowing not only environmental factors but also cellular and molecular states of eDNA in water. Further verification of the relationship between eDNA state and persistence is required by obtaining more information on eDNA persistence in various experimental and environmental conditions, which will enhance our knowledge on eDNA persistence and support our findings.

  Last, Jul. 2021, Molecular Ecology Resources, 21(5) (5), 1490 - 1503, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimating fish population abundance by integrating quantitative data on environmental DNA and hydrodynamic modelling

  Keiichi Fukaya, Hiroaki Murakami, Seokjin Yoon, Kenji Minami, Yutaka Osada, Satoshi Yamamoto, Reiji Masuda, Akihide Kasai, Kazushi Miyashita, Toshifumi Minamoto, Michio Kondoh

  Wiley, Jul. 2021, Molecular Ecology, 30(13) (13), 3057 - 3067, English

  [Refereed]

  Scientific journal


• Utility of environmental DNA analysis for effective monitoring of invasive fish species in reservoirs

  Toshiaki Jo, Saki Ikeda, Arisa Fukuoka, Takashi Inagawa, Jiro Okitsu, Izumi Katano, Hideyuki Doi, Katsuki Nakai, Hidetaka Ichiyanagi, Toshifumi Minamoto

  Last, Wiley, Jun. 2021, Ecosphere, 12(6) (6), e03643, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples

  Tetsu Yasashimoto, Masayuki K. Sakata, Tomoya Sakita, Satoko Nakajima, Mamiko Ozaki, Toshifumi Minamoto

  Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species.

  Springer Science and Business Media LLC, May 2021, Scientific Reports, 11(1) (1), 10712, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Molecular detection of giant snakeheads, Channa micropeltes (Cuvier, 1831), one of the most troublesome fish species

  Maslin Osathanunkul, Toshifumi Minamoto

  A lack of reliable tools for determining the presence and distribution of fish species can impede understanding of predator–prey interactions and fishery management. Conventional fish survey methods are invasive, and can be size or species selective. Combining netting and electrofishing is a current method used to monitor fish species in Phayao Lake (Kwan Phayao), Thailand. However, the methods are inefficient and time-consuming. Recently, locals who rely on inland fisheries in Kwan Phayao expressed their deep concerns about the giant snakehead, Channa micropeltes (Cuvier, 1831) destroying other fish there. The giant snakehead prey on many commercially important fish species, as the prey species is reduced, negative effects on both biodiversity and the fishery sector could follow. Here, an eDNA-based survey was developed to detect the presence of the giant snakehead. Water samples were collected from six sites within Kwan Phayao and 17 sites in Ing River where water flowed into and out of Kwan Payao. The eDNA of the giant snakehead was detected in water samples from all collection sites using the developed qPCR assay with various concentrations. The eDNA was shown here to be a sensitive and reliable tool for fish surveillance so there will be a better chance for developing an effective management strategy.

  Springer Science and Business Media LLC, May 2021, Scientific Reports, 11(1) (1), 9943, English

  [Refereed]

  Scientific journal


• Spatiotemporal distribution of Flavobacterium psychrophilum and ayu Plecoglossus altivelis in rivers revealed by environmental DNA analysis

  Hinako Tenma, Koki Tsunekawa, Riho Fujiyoshi, Hajime Takai, Masae Hirose, Nanami Masai, Kosuke Sumi, Yuta Takihana, Sogen Yanagisawa, Kota Tsuchida, Kenichi Ohara, Toshiaki Jo, Masaki Takagi, Akiko Ota, Hiroyoshi Iwata, Yuichi Yaoi, Toshifumi Minamoto

  May 2021, Fisheries Science, 87(3) (3), 321 - 330, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Compilation of real‐time PCR conditions toward the standardization of environmental DNA methods

  Hideyuki Doi, Toshifumi Minamoto, Teruhiko Takahara, Satsuki Tsuji, Kimiko Uchii, Satoshi Yamamoto, Izumi Katano, Hiroki Yamanaka

  Wiley, May 2021, Ecological Research, 36(3) (3), 379 - 388, English

  [Refereed]

  Scientific journal


• Determining an effective sampling method for eDNA metabarcoding: a case study for fish biodiversity monitoring in a small, natural river

  Masayuki K. Sakata, Takeshi Watanabe, Nobutaka Maki, Kousuke Ikeda, Toshihiro Kosuge, Hiroaki Okada, Hiroki Yamanaka, Tetsuya Sado, Masaki Miya, Toshifumi Minamoto

  Last, Apr. 2021, Limnology, 22(2) (2), 221 - 235, English

  [Refereed]

  Scientific journal


• Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique

  Tatsuhiko Hoshino, Ryohei Nakao, Hideyuki Doi, Toshifumi Minamoto

  The combination of high-throughput sequencing technology and environmental DNA (eDNA) analysis has the potential to be a powerful tool for comprehensive, non-invasive monitoring of species in the environment. To understand the correlation between the abundance of eDNA and that of species in natural environments, we have to obtain quantitative eDNA data, usually via individual assays for each species. The recently developed quantitative sequencing (qSeq) technique enables simultaneous phylogenetic identification and quantification of individual species by counting random tags added to the 5′ end of the target sequence during the first DNA synthesis. Here, we applied qSeq to eDNA analysis to test its effectiveness in biodiversity monitoring. eDNA was extracted from water samples taken over 4 days from aquaria containing five fish species (Hemigrammocypris neglectus, Candidia temminckii, Oryzias latipes, Rhinogobius flumineus, and Misgurnus anguillicaudatus), and quantified by qSeq and microfluidic digital PCR (dPCR) using a TaqMan probe. The eDNA abundance quantified by qSeq was consistent with that quantified by dPCR for each fish species at each sampling time. The correlation coefficients between qSeq and dPCR were 0.643, 0.859, and 0.786 for H. neglectus, O. latipes, and M. anguillicaudatus, respectively, indicating that qSeq accurately quantifies fish eDNA.

  Springer Science and Business Media LLC, Feb. 2021, Scientific Reports, 11(1) (1), 4372, English

  [Refereed]

  Scientific journal


• Continuous prevalence of VEB‐3 extended‐spectrum β‐lactamase‐producing Aeromonas hydrophila in a local river in gifu city, Japan

  Tomoki Aratani, Nanami Koide, Kana Hayami, Michiyo Sugiyama, Toshifumi Minamoto, Tetsuo Asai

  Wiley, Feb. 2021, Microbiology and Immunology, 65(2) (2), 99 - 100, English, International magazine

  Scientific journal


• Broad-scale detection of environmental DNA for an invasive macrophyte and the relationship between DNA concentration and coverage in rivers

  Hideyuki Doi, Yoshihisa Akamatsu, Masuji Goto, Ryutei Inui, Takashi Komuro, Mariko Nagano, Toshifumi Minamoto

  Lead, Springer Science and Business Media LLC, Feb. 2021, Biological Invasions, 23(2) (2), 507 - 520, English

  [Refereed]

  Scientific journal


• Messenger RNA typing of environmental RNA (eRNA): A case study on zebrafish tank water with perspectives for the future development of eRNA analysis on aquatic vertebrates

  Kenji Tsuri, Shizuya Ikeda, Takaya Hirohara, Yasuhito Shimada, Toshifumi Minamoto, Hiroki Yamanaka

  Wiley, Jan. 2021, Environmental DNA, 3(1) (1), 14 - 21, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols

  Toshifumi Minamoto, Masaki Miya, Tetsuya Sado, Satoquo Seino, Hideyuki Doi, Michio Kondoh, Keigo Nakamura, Teruhiko Takahara, Satoshi Yamamoto, Hiroki Yamanaka, Hitoshi Araki, Wataru Iwasaki, Akihide Kasai, Reiji Masuda, Kimiko Uchii

  Wiley, Jan. 2021, Environmental DNA, 3(1) (1), 8 - 13, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Characterizing the spatial and temporal occurrence patterns of the endangered botiid loach Parabotia curtus by environmental DNA analysis using a newly developed species-specific primer set

  Ko Sugiura, Sei Tomita, Toshifumi Minamoto, Tappei Mishina, Akihisa Iwata, Tsukasa Abe, Satoshi Yamamoto, Katsutoshi Watanabe

  Springer Science and Business Media LLC, Jan. 2021, Ichthyological Research, 68(1) (1), 152 - 157, English

  [Refereed]

  Scientific journal


• An Optional Low-Cost Method of Extracting Environmental DNA of Macro-Organisms from Filter Membranes in Large Scale eDNA Surveys

  Zhi Chen, Toshifumi Minamoto, Longshan Lin, Tianxiang Gao

  ResearchersLinks Ltd, Jan. 2021, Pakistan Journal of Zoology, 53(1) (1), 253 - 272, English

  [Refereed]

  Scientific journal


• Revealing an invasion risk of fish species in Qingdao Underwater World by Eevironmental DNA metabarcoding

  Jianwei Chen, Zhi Chen, Shanshan Liu, Wenjie Guo, Di Li, Toshifumi Minamoto, Tianxiang Gao

  Springer Science and Business Media LLC, Jan. 2021, Journal of Ocean University of China, 20(1) (1), 124 - 136, English

  [Refereed]

  Scientific journal


• A molecular survey based on eDNA to assess the presence of a clown featherback (Chitala ornata) in a confined environment

  Maslin Osathanunkul, Toshifumi Minamoto

  Last, Dec. 2020, PeerJ, 8, e10338, English

  [Refereed]

  Scientific journal


• Seasonal monitoring of Hida salamander Hynobius kimurae using environmental DNA with a genus-specific primer set

  Toshiaki Jo, Sei Tomita, Yukihiro Kohmatsu, Maslin Osathanunkul, Atushi Ushimaru, Toshifumi Minamoto

  The diversity and the abundance of amphibians have dramatically declined globally over the past 30 years, and the monitoring and conservation of their habitats is essential. However, traditional methods such as bait trapping and mark-recapture are costly, and morphological identification usually requires a high level of taxonomic expertise. Here, seasonal surveillances of Hida salamander Hynobius kimurae were performed by means of environmental DNA (eDNA) analysis with Hynobius-specific primers and a species-specific TaqMan probe. Water sampling and visual surveys were conducted seasonally in a stream in Kyoto Prefecture, Japan. Detection rates of eDNA were then calculated by real-time PCR, and eDNA site occupancy probability was estimated by multi-scale occupancy modeling. The eDNA-based detection rate of Hida salamander was 76.7%, whereas the visual survey-based detection rate was 23.3%, and target eDNA was detected at almost all sites where the presence of target species was visually confirmed. Moreover, factors relating to the site- and sample-level occurrence probabilities of the target eDNA differed depending on the developmental stage of the target species. Our findings support previous studies showing that eDNA analysis enables an effective assessment of amphibian distributions without damaging the organisms or their habitat, and we compare for the first time the site occupancy probability of amphibian eDNA throughout the life cycle of an amphibian species. The present study contributes to the development of eDNA analysis as a tool for understanding the distribution and seasonal activity of amphibian species and will thus aid in the planning of conservation measures and habitat restoration for these species.

  Inter-Research Science Center, Nov. 2020, Endangered Species Research, 43, 341 - 352, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Feasibility study for seagrass beds monitoring using environmental DNA

  Edwin Muchebve, Yuriko Takayama, Maiko Akatsuka, Kazunori Ito, Toshifumi Minamoto

  Last, Japan Society of Civil Engineers, Nov. 2020, Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering), 76(2) (2), I_949 - I_954, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Seasonal changes of environmental DNA in seagrass beds and examination of water sampling procedure

  Maiko Akatsuka, Yuriko Takayama, Edwin Muchevbe, Kazunori Ito, Kenta Watanabe, Tomohiro Kuwae, Toshifumi Minamoto

   The utilization of environmental DNA(eDNA) is being considered as a new method for monitoring seagrass beds. To understand the change in eDNA corresponding to seasonal variations in seagrass, the growth of Zostera marina inhabiting inside a water tank along with the amount of eDNA were investigated for fifteen months. Furthermore, investigations were performed under conditions where the flow changes due to the tide for the future monitoting in the sea. The investigations focused on the differernce of the eDNA collected at different sampling points and at the same time of a day on the seasonal changes.

   The fifteen month monitoring confirmed that the concentration of eDNA was high in the summer season which suggests that it is related to the increase in the amount of seagrass biomass and its release. As a result of the survey in the sea, the samples taken at the same time of a day showed a mixture of detection and non-detection. However, by increasing the amount of water sampled by 10 times, and the amount of analysis by 2.5times, and also by reducing the inhibition effect, detection was possible. Additionally, the results showed the possibility of applying the method in this study for future surveys in the sea.

  Last, Japan Society of Civil Engineers, Nov. 2020, Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering), 76(2) (2), I_943 - I_948, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA revealed the fish community of Hokkaido Island, Japan, after invasion by rainbow trout

  Akio Imamura, Kana Hayami, Masayuki K. Sakata, Toshifumi Minamoto

  In freshwater ecosystems, invasive salmonid fishes can have a significant impact on native fish species. Detecting the invasion and its negative effects is critical for the conservation of native fish communities. We examined the species composition and seasonal changes in the freshwater fish community, including salmonids, on the Kamikawa Plain, Hokkaido Island, Japan, using environmental DNA (eDNA) metabarcoding. We detected 23 fish species in 176 samples collected from 16 sites over 12 months (October 2018 – August 2019). Between 11 and 20 species were detected at each site, including five native salmonids (Oncorhynchus masou, Oncorhynchus keta, Parahucho perryi, Salvelinus leucomaenis leucomaenis and Salvelinus malma krascheninnikova). The invasive alien rainbow trout Oncorhynchus mykiss was detected at all 16 sites and it was the most commonly detected salmonid. Although we found no obvious competitive exclusion of native salmonids by rainbow trout in the study area, the invasive species occurred more often and at more sites than any of the natives. We also determined the occurrence and seasonal changes in the fish community, classified as native salmonids, invasive rainbow trout, Cypriniformes and other benthic fishes. There were fewer species overall in winter, but the sites with higher species richness in winter were on the lower reaches of the river. In addition, we detected domestic invaders, such as the topmouth gudgeon, Pseudorasbora parva, although they were less prevalent than rainbow trout. These results show the effectiveness of eDNA metabarcoding, which can be used for surveying species richness at an ecosystem scale. In particular, the detection of the early stages of establishment and spread of invasive species can be achieved by eDNA monitoring.

  Last, Pensoft Publishers, Oct. 2020, Biodiversity Data Journal, 8, e56876, English, International magazine

  [Refereed]

  Scientific journal


• Sedimentary DNA tracks decadal-centennial changes in fish abundance

  Michinobu Kuwae, Hiromichi Tamai, Hideyuki Doi, Masayuki K. Sakata, Toshifumi Minamoto, Yoshiaki Suzuki

  Far too little is known about the long-term dynamics of populations for almost all macro-organisms. Here, we examined the utility of sedimentary DNA techniques to reconstruct the dynamics in the “abundance” of a species, which has not been previously defined. We used fish DNA in marine sediments and examined whether it could be used to track the past dynamics of pelagic fish abundance in marine waters. Quantitative PCR for sedimentary DNA was applied on sediment-core samples collected from anoxic bottom sediments in Beppu Bay, Japan. The DNA of three dominant fish species (anchovy, sardine, and jack mackerel) were quantified in sediment sequences spanning the last 300 years. Temporal changes in fish DNA concentrations are consistent with those of landings in Japan for all three species and with those of sardine fish scale concentrations. Thus, sedimentary DNA could be used to track decadal-centennial dynamics of fish abundance in marine waters.

  Springer Science and Business Media LLC, Oct. 2020, Communications Biology, 3(1) (1), 558, English

  [Refereed]

  Scientific journal


• Detection of herbivory: eDNA detection from feeding marks on leaves

  Aoi Kudoh, Toshifumi Minamoto, Satoshi Yamamoto

  Wiley, Oct. 2020, Environmental DNA, 2(4) (4), 627 - 634, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA

  Masayuki K. Sakata, Satoshi Yamamoto, Ryo O. Gotoh, Masaki Miya, Hiroki Yamanaka, Toshifumi Minamoto

  Last, Wiley, Oct. 2020, Environmental DNA, 2(4) (4), 505 - 518, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Selective collection of long fragments of environmental DNA using larger pore size filter

  Toshiaki Jo, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

  Last, Elsevier BV, Sep. 2020, Science of The Total Environment, 735, 139462 - 139462, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA analysis shows high potential as a tool for estimating intraspecific genetic diversity in a wild fish population

  Satsuki Tsuji, Atsushi Maruyama, Masaki Miya, Masayuki Ushio, Hirotoshi Sato, Toshifumi Minamoto, Hiroki Yamanaka

  Wiley, Sep. 2020, Molecular Ecology Resources, 20(5) (5), 1248 - 1258, English

  [Refereed]

  Scientific journal


• Projection range of eDNA analysis in marshes: a suggestion from the Siberian salamander (Salamandrella keyserlingii) inhabiting the Kushiro marsh, Japan

  Daiki Takeshita, Shigeharu Terui, Kousuke Ikeda, Takashi Mitsuzuka, Maslin Osathanunkul, Toshifumi Minamoto

  Freshwater ecosystems are rapidly declining. The Siberian salamander (Salamandrella keyserlingii) which inhabits the Kushiro marsh in Hokkaido, Japan has lost some habitat due to human activity. There are many challenges associated with conventional monitoring methods, including cost, the need for specialist personnel, environmental impact, and ability to detect the presence of this species; thus, we investigated the feasibility of using environmental DNA (eDNA) analysis to detect its presence and identify its breeding grounds. We performed tank experiments to confirm eDNA emission from egg sacs, larvae, and adult Siberian salamanders in the water. We also performed water sampling and visual observation of egg sacs in the Kushiro marsh during the end of the breeding season and the larval season. The tank experiments found eDNA emission from all growth stages. It also implied concentrated emissions just after spawning and after hatching, and limited emissions during the incubation phase in egg sacs. We also detected eDNA in the field, likely reflecting the distribution of egg sacs or larvae. Combining this data with visual observations, it was determined that the eDNA results from the field were best explained by the number of egg sacs within 7–10 m of the sampling point. The results of this investigation show that the breeding sites and habitats of marshland species can successfully be monitored using eDNA analysis. They also suggest that the eDNA results from the marshes may reflect the biomass that is in close range to the sampling point. These results support the increased use of eDNA analysis in marshes and provide knowledge that could improve the interpretation of future results.

  PeerJ, Aug. 2020, PeerJ, 8, e9764 - e9764, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Suppression of environmental DNA degradation in water samples associated with different storage temperature and period using benzalkonium chloride

  Teruhiko Takahara, Junya Taguchi, Satoshi Yamagishi, Hideyuki Doi, Shigeki Ogata, Hiroki Yamanaka, Toshifumi Minamoto

  Wiley, Jul. 2020, Limnology and Oceanography: Methods, 18(8) (8), 437 - 445, English

  [Refereed]

  Scientific journal


• Estimations of riverine distribution, abundance and biomass of anguillid eels in Japan and Taiwan using environmental DNA analysis

  Hikaru Itakura, Ryoshiro Wakiya, Masayuki K. Sakata, Hsiang-Yi Hsu, Shih-Chong Chen, Chih-shao Yang, Satoshi Yamamoto, Toshifumi Minamoto

  Although populations of anguillid eels have declined remarkably in recent decades, monitoring data on the spatial and temporal variation in their dynamics are often limited, particularly for tropical eel species. As there are often sympatries of multiple eel species in tropical rivers, identifying eel species based solely on morphological characteristics is challenging. Basin-scale surveys were conducted in rivers of southern Japan and northern Taiwan to investigate (1) whether the spatial distribution, abundance, and biomass of the tropical eel species, the giant mottled eel (Anguilla marmorata), can be monitored in rivers by comparing the results obtained from environmental DNA (eDNA) analysis with data from electrofishing and (2) the riverine distribution of the sympatric A. marmorata and the temperate eel species, the Japanese eel (Anguilla japonica), in this region using eDNA analysis. Although we found an much lower abundance of A. marmorata in the study region, we identified the eDNA of the species from all of the study sites (21 sites) where it was collected by electrofishing, in addition to 22 further study sites where it was not collected directly. This indicates that eDNA analysis has a greater sensitivity for detecting A. marmorata, making it a powerful tool for monitoring the spatial distribution of the species in rivers. We found a significant positive relationship between eDNA concentration and both the abundance and biomass of A. marmorata, and eDNA concentration seemed to better reflect the abundance of the species than did biomass. eDNA of both A. japonica and A. marmorata was identified from almost all rivers, indicating the sympatry of these species in this region, although the degree of sympatry differed between rivers. Though the eDNA concentration of A. japonica decreased significantly with increasing distance from the river mouth, no significant relationship was found for A. marmorata. This study is the first to demonstrate the potential usefulness of eDNA analysis for estimating the spatial distribution, abundance, and biomass of tropical eels in rivers and to further apply this method to investigate sympatry among anguillid species. eDNA analysis can help in obtaining data on the population dynamics of tropical eels, providing invaluable information for managing these species.

  Jun. 2020, Zoological Studies, 59, 17, English, International magazine

  [Refereed]

  Scientific journal


• Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs

  Kana Hayami, Masayuki K. Sakata, Takashi Inagawa, Jiro Okitsu, Izumi Katano, Hideyuki Doi, Katsuki Nakai, Hidetaka Ichiyanagi, Ryo O. Gotoh, Masaki Miya, Hirotoshi Sato, Hiroki Yamanaka, Toshifumi Minamoto

  Last, Wiley, May 2020, Ecology and Evolution, 10(11) (11), 5354 - 5367, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparing the efficiency of open and enclosed filtering systems in fish and jellyfish environmental DNA quantification

  Sayaka Takahashi, Masayuki K. Sakata, Toshifumi Minamoto, Reiji Masuda

  Water sampling and filtration of environmental DNA (eDNA) analysis have been performed by several different methods, and each method may yield a different species composition or eDNA concentration. Here, we investigated the eDNA of seawater samples directly collected by SCUBA to compare two widely used filtration methods: open filtration with a glass filter (GF/F) and enclosed filtration (Sterivex). We referred to biomass based on visual observation data collected simultaneously to clarify the difference between organism groups. Water samples were collected at two points in the Sea of Japan in May, September and December 2018. The respective samples were filtered through GF/F and Sterivex for eDNA extraction. We quantified the eDNA concentration of five fish and two cnidarian species by quantitative polymerase chain reaction (qPCR) using species-specific primers/probe sets. A strong correlation of eDNA concentration was obtained between GF/F and Sterivex; the intercepts and slopes of the linear regression lines were slightly different in fish and jellyfish. The amount of eDNA detected using the GF/F filtration method was higher than that detected using Sterivex when the eDNA concentration was high; the opposite trend was observed when the eDNA concentration was relatively low. The concentration of eDNA correlated with visually estimated biomass; eDNA concentration per biomass in jellyfish was approximately 700 times greater than that in fish. We conclude that GF/F provides an advantage in collecting a large amount of eDNA, whereas Sterivex offers superior eDNA sensitivity. Both filtration methods are effective in estimating the spatiotemporal biomass size of target marine species.

  Apr. 2020, PLOS ONE, 15(4) (4), e0231718, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimating shedding and decay rates of environmental nuclear DNA with relation to water temperature and biomass

  Toshiaki Jo, Mio Arimoto, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

  Wiley, Apr. 2020, Environmental DNA, 2(2) (2), 140 - 151, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt ( Spirinchus lanceolatus )

  Tetsu Yatsuyanagi, Ryotaro Ishida, Masayuki K. Sakata, Takashi Kanbe, Hiroki Mizumoto, Yumi Kobayashi, Shoko Kamada, Satoko Namba, Hisaya Nii, Toshifumi Minamoto, Hitoshi Araki

  Wiley, Apr. 2020, Environmental DNA, 2(2) (2), 130 - 139

  [Refereed]

  Scientific journal


• Multiplex real-time PCR enables the simultaneous detection of environmental DNA from freshwater fishes: a case study of three exotic and three threatened native fishes in Japan

  Toshiaki Jo, Arisa Fukuoka, Kei Uchida, Atushi Ushimaru, Toshifumi Minamoto

  Environmental DNA (eDNA) analysis has been used as a cost-efficient and non-invasive tool for monitoring invasive and threatened species. Previous studies typically involve two approaches; species-specific detection via PCR and multiple species detection via metabarcoding. However, the former could be costly when several species are targeted, and the latter could sometimes be insufficient to distinguish closely related species. Here, the simultaneous eDNA detection from multiple species via multiplex real-time PCR was applied to 99 ponds to evaluate the distribution of three exotic and three threatened native fish species over different seasons. We detected bluegill sunfish (Lepomis macrochirus) eDNA at 31 sites, largemouth bass (Micropterus salmoides) eDNA at 22 sites, smallmouth bass (Micropterus dolomieu) eDNA at one site, golden venus chub (Hemigrammocypris rasborella) eDNA at 11 sites, Japanese medaka (Oryzias latipes) eDNA at 26 sites, and weather loach (Misgurnus anguillicaudatus) eDNA at 41 sites. We found that eDNA detection rates were higher in early summer for all fish species. Moreover, exotic fish eDNA was detected more frequently in ponds which were easier to access by car and which have a larger surface area and higher pH. Furthermore, the detection rates of native fish eDNA were generally lower in the ponds where exotic fish eDNA was detected more frequently. Multiplex real-time PCR can help detect the distribution of exotic and threatened native species for conservation and ecosystem management. This method is expected to substantially contribute to the early detection of invasive species and the efficient protection of threatened species' habitat.

  SPRINGER, Feb. 2020, Biological Invasions, 22(2) (2), 455 - 471, English

  [Refereed]

  Scientific journal


• The eDNA Collection method of Zhoushan coastal waters

  CHEN Zhi, SONG Na, MINAMOTO Toshifumi, WU Qian-Qian, GAO Tian-Xiang

  Jan. 2020, Acta Hydrobiologica Sinica, 44(1) (1), Chinese

  [Refereed]

  Scientific journal


• Evaluating intraspecific genetic diversity using environmental DNA and denoising approach: A case study using tank water

  Satsuki Tsuji, Masaki Miya, Masayuki Ushio, Hirotoshi Sato, Toshifumi Minamoto, Hiroki Yamanaka

  Wiley, Jan. 2020, Environmental DNA, 2(1) (1), 42 - 52, English

  [Refereed]

  Scientific journal


• Environmental DNA detection and quantification of invasive red-eared sliders, Trachemy scripta elegans, in ponds and the influence of water quality

  Aozora Kakuda, Hideyuki Doi, Rio Souma, Mariko Nagano, Toshifumi Minamoto, Izumi Katano

  Environmental DNA (eDNA) is a powerful tool for monitoring the distribution of aquatic macro-organisms. However, environmental factors, including the water temperature and water quality, can affect the inhibition and/or degradation of eDNA, which complicates accurate estimations of eDNA concentrations and the detection of the presence/absence of species in natural habitats. Further very few eDNA studies have been conducted for reptiles, especially with respect to estimating their biomass and/or abundances. Here we examined the relationship between the visually-observed number of red-eared sliders (Trachemys scripta elegans) and eDNA concentrations across 100 ponds. Additionally, we evaluated the effect of water quality on red-eared slider eDNA concentration in these ponds. We found that there was a significant positive correlation between the observed number of red-eared sliders and the eDNA concentration in the ponds. On comparing various water quality indicators, including dissolved nitrogen, dissolved phosphorous, organic matter, and chlorophyll a (Chl. a), we found that only Chl. a had a negative correlation with the red-eared slider eDNA concentration, while we did not find any inhibition in the quantitative PCR. We conclude that concentrations of eDNA can potentially be used for estimating the abundance of the red-eared slider. Additionally, Chl. a might indirectly influence the degradation of eDNA through the microorganisms bonded to the phytoplankton in the ponds, as microbial activity is thought to decrease eDNA persistence.

  PeerJ, Dec. 2019, PeerJ, 7, e8155 - e8155, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan

  Yukuto Sato, Masaru Mizuyama, Megumi Sato, Toshifumi Minamoto, Ryosuke Kimura, Claudia Toma

  Springer Science and Business Media LLC, Dec. 2019, Scientific Reports, 9(1) (1), 6575, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines

  Raffy Jay C. Fornillos, Marcello Otake Sato, Ian Kim B. Tabios, Megumi Sato, Lydia R. Leonardo, Yuichi Chigusa, Toshifumi Minamoto, Mihoko Kikuchi, Emelda R. Legaspi, Ian Kendrich C. Fontanilla

  In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection.

  Nov. 2019, PLOS ONE, 14(11) (11), e0224617, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA

  Kimiko Uchii, Hideyuki Doi, Teruyuki Okahashi, Izumi Katano, Hiroki Yamanaka, Masayuki K. Sakata, Toshifumi Minamoto

  Wiley, Nov. 2019, Environmental DNA, 1(4) (4), 359 - 367, English

  [Refereed]

  Scientific journal


• A feasibility study in a monitoring method of non-moving aquatic organism using environmental DNA

  TAMAYAMA Yuriko, AKATSUKA Maiko, ITO Kazunori, MINAMOTO Toshifumi

  Last, Nov. 2019, Journal of Japan Society of Civil Engineers (B2), 75(2) (2), I_1087 - I_1092, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• STUDY ON SEASONAL, DAILY AND MORPHOLOGICAL CHANGE OF ENVIRONMENTAL DNA FOR SEAGRASS BED

  AKATSUKA Maiko, TAKAYAMA Yuriko, ITO Kazunori, WATANABE Kenta, KUWAE Tomohiro, OSAWA Ryosuke, MORIMOTO Teppei, MINAMOTO Toshifumi

  Last, 土木学会, Nov. 2019, Journal of Japan Society of Civil Engineers (B2), 75(2) (2), I_1075 - 1080, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi

  Fritz Ivy C. Calata, Camille Z. Caranguian, Jillian Ela M. Mendoza, Raffy Jay C. Fornillos, Ian Kim B. Tabios, Ian Kendrich C. Fontanilla, Lydia R. Leonardo, Louie S. Sunico, Satoru Kawai, Yuichi Chigusa, Mihoko Kikuchi, Megumi Sato, Toshifumi Minamoto, Zenaida G. Baoanan, Marcello Otake Sato

  Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.

  MDPI AG, Sep. 2019, Pathogens, 8(4) (4), 160 - 160, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Seasonal change in environmental DNA concentration of a submerged aquatic plant species

  Saeko Matsuhashi, Toshifumi Minamoto, Hideyuki Doi

  Sep. 2019, Freshwater Science, 38, 654 - 660, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Discovery of an unrecorded population of Yamato salamander ( Hynobius vandenburghi ) by GIS and eDNA analysis

  Yusuke Sakai, Ayane Kusakabe, Kota Tsuchida, Yuka Tsuzuku, Shogo Okada, Takuto Kitamura, Sei Tomita, Takahiko Mukai, Masataka Tagami, Masaki Takagi, Yuichi Yaoi, Toshifumi Minamoto

  Wiley, Sep. 2019, Environmental DNA, 1(3) (3), 281 - 289, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Particle Size Distribution of Environmental DNA from the Nuclei of Marine Fish

  Toshiaki Jo, Mio Arimoto, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

  Last, Aug. 2019, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 53(16) (16), 9947 - 9956, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Biomass‐dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles

  Tomoya Horiuchi, Reiji Masuda, Hiroaki Murakami, Satoshi Yamamoto, Toshifumi Minamoto

  Wiley, Jul. 2019, Journal of Fish Biology, 95(3) (3), 979 - 981, English

  [Refereed]

  Scientific journal


• How science, technology and innovation can be placed in broader visions — Public opinions from inclusive public engagement activities

  Kei Kano, Mitsuru Kudo, Go Yoshizawa, Eri Mizumachi, Makiko Suga, Naonori Akiya, Kuniyoshi Ebina, Takayuki Goto, Masayuki Itoh, Ayami Joh, Haruhiko Maenami, Toshifumi Minamoto, Mikihiko Mori, Yoshitaka Morimura, TAMAKI Motoki, Akie Nakayama, Katsuya Takanashi

  Jun. 2019, Journal of Science Communication, 18(03) (03), A02, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Are Salvelinus species on Hokkaido Island, Japan, endangered by damming and invasive rainbow trouts? ~based on the results of eDNA analysis

  IMAMURA Akio, HAYAMI Kana, SAKATA Masayuki, K, MINAMOTO Toshifumi

  We investigated the distributions of two native (Dolly Varden Salvelinus malma malma and whitespotted char Salvelinus leucomaenis leucomaenis) and one invasive (rainbow trout Oncorhynchus mykiss) salmonid species using environmental DNA (eDNA) analysis in the centre of Hokkaido Island, Japan. The native species' populations are fragmented by damming and threatened by invasive species. Therefore, DNA real-time PCR assays specific to these three salmonids were used to investigate the effects of damming and invasive species on the two native salmonids. Salvelinus malma populations exhibited separation due to damming. Additionally, they were not eliminated by invasive O. mykiss but rather lived together at some sites. Salvelinus leucomaenis populations occupied the lower reaches more than did S. malma populations. We detected S. leucomaenis and the invasive O. mykiss population less frequently than expected. We were unable to clarify the seasonal movements of species, even during their reproductive phase, despite conducting eDNA surveys throughout the year, including during the coldest parts of winter. We hypothesise that damming may function both as a protective barrier against invasive species and as an impassable barrier preventing migration; however, the significance of these potential functions was not revealed in this study. From a long-term perspective, fragmentation may negatively affect the viability of native Salvelinus populations. Conservation efforts for native Salvelinus species would be aided by additional studies using eDNA surveys, which can be effectively conducted even in mid-winter.

  Last, The Ecological Society of Japan, May 2019, Japanese Journal of Conservation Ecology, 24(1) (1), 71 - 81, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan—An eDNA‐based study

  Qianqian Wu, Ken Kawano, Toshiyuki Ishikawa, Masayuki K. Sakata, Ryohei Nakao, Masayoshi K. Hiraiwa, Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  Last, Wiley, May 2019, Environmental DNA, 1(1) (1), 54 - 63, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The life history with seasonal migration of the lacustrine shrimp Palaemon paucidens in an ancient lake in Japan

  Qianqian Wu, Yasuoki Takami, Toshifumi Minamoto, Toshiyuki Ishikawa

  Apr. 2019, Ecosphere, 10(4) (4), e02628, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Using environmental DNA to estimate the seasonal distribution and habitat preferences of a Japanese basket clam in Lake Shinji, Japan

  Teruhiko Takahara, Takashi Ikebuchi, Hideyuki Doi, Toshifumi Minamoto

  Mar. 2019, Estuarine, Coastal and Shelf Science, 221, 15 - 20, English

  [Refereed]

  Scientific journal


• Environmental DNA analysis reveals the spatial distribution, abundance, and biomass of Japanese eels at the river‐basin scale

  Hikaru Itakura, Ryoshiro Wakiya, Satoshi Yamamoto, Kenzo Kaifu, Takuya Sato, Toshifumi Minamoto

  Wiley, Mar. 2019, Aquatic Conservation: Marine and Freshwater Ecosystems, 29(3) (3), 361 - 373, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Dispersion and degradation of environmental DNA from caged fish in a marine environment

  Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda

  Springer Science and Business Media LLC, Mar. 2019, Fisheries Science, 85(2) (2), 327 - 337, English

  [Refereed]

  Scientific journal


• Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution

  Toshiaki Jo, Hiroaki Murakami, Satoshi Yamamoto, Reiji Masuda, Toshifumi Minamoto

  Last, Feb. 2019, ECOLOGY AND EVOLUTION, 9(3) (3), 1135 - 1146, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Real-time PCR assays for environmental DNA detection of three salmonid fish in Hokkaido, Japan: application to winter surveys

  Toshifumi Minamoto, Kana Hayami, Masayuki K. Sakata, Akio Imamura

  Jan. 2019, Ecological Research, 34(1) (1), 237 - 242, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• First use of oceanic environmental DNA to study the spawning ecology of the Japanese eel Anguilla japonica

  Aya Takeuchi, Shun Watanabe, Satoshi Yamamoto, Michael J. Miller, Tatsuhiro Fukuba, Tstsuya Miwa, Tatsufumi Okino, Toshifumi Minamoto, Katsumi Tsukamoto

  Inter-Research Science Center, Jan. 2019, Marine Ecology Progress Series, 609, 187 - 196, English

  [Refereed]

  Scientific journal


• Usefulness of environmental DNA for detecting Schistosoma mansoni occurrence sites in Madagascar

  Marcello Otake Sato, Armand Rafalimanantsoa, Charles Ramarokoto, Alain Marcel Rahetilahy, Pascaline Ravoniarimbinina, Satoru Kawai, Toshifumi Minamoto, Megumi Sato, Masashi Kirinoki, Voahangy Rasolofo, Mathilde De Calan, Yuichi Chigusa

  Elsevier BV, Nov. 2018, International Journal of Infectious Diseases, 76, 130 - 136, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Study on appropriate environmental DNA and applicability of tidal flow analysis for seagrass bed

  AKATSUKA Maiko, TAKAYAMA Yuriko, ITO Kazunori, MORIMOTO Teppei, MINAMOTO Toshifumi

  Nov. 2018, Journal of Japan Society of Civil Engineers (B2), 74(2) (2), I_1225 - I_1230, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A feasibility study in a monitoring method of seagrass bed using environmental DNA.

  TAMAYAMA Yuriko, AKATSUKA Maiko, ITO Kazunori, MINAMOTO TOSHIFUMI

  Nov. 2018, Journal of Japan Society of Civil Engineers (B2), 74(2) (2), I_1231 - I_1236, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA reveals nonmigratory individuals of Palaemon paucidens overwintering in Lake Biwa shallow waters

  Qianqian Wu, Ken Kawano, Yoshitoshi Uehara, Noboru Okuda, Masamichi Hongo, Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  Last, University of Chicago Press, Jun. 2018, Freshwater Science, 37(2) (2), 307 - 314, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparing local- and regional-scale estimations of the diversity of stream fish using eDNA metabarcoding and conventional observation methods

  Hikaru Nakagawa, Satoshi Yamamoto, Yukuto Sato, Tetsuya Sado, Toshifumi Minamoto, Masaki Miya

  Blackwell Publishing Ltd, Jun. 2018, Freshwater Biology, 63(6) (6), 569 - 580, English

  [Refereed]

  Scientific journal


• Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing

  Masayuki Ushio, Hiroaki Murakami, Reiji Masuda, Tetsuya Sado, Masaki Miya, Sho Sakurai, Hiroki Yamanaka, Toshifumi Minamoto, Michio Kondoh

  Mar. 2018, Metabarcoding & Metagenomics, 2018, e23297, English

  [Refereed]

  Scientific journal


• Detecting river fish using environmental DNA analysis and a conventional field sampling survey at 500-m intervals

  Niwa Hideyuki, Sakata Masayuki K., Minamoto Toshifumi, Kiyono Mieko

  Environmental DNA (eDNA) analysis for macro-organisms has developed rapidly, and studies have reported the application of such analyses to various kinds of organism. However, only a few studies have compared the results of eDNA analysis and traditional sampling surveys on a fine scale, and the usefulness of eDNA analysis for understanding the distribution of endangered species on a finer scale has not been sufficiently assessed. In this study, we conducted eDNA analysis and a traditional sampling survey in multiple 500-m sections of a river and compared the results. The survey targets were the endangered fish species Liobagrus reinii, Coreoperca kawamebari, and Lethenteron sp. 2, which are known to occur in the surveyed river. We established 23, 500-m-long sections in the Sasayama River and sampled water at the downstream end of each section. The eDNA of the target species was detected using real-time PCR assays developed in this study. We also collected the target species in traditional sampling surveys and recorded the presence/absence of the target species in each section. The rates of concordance between the results of the eDNA analysis and the traditional sampling surveys varied among the species. Our result showed that the results of eDNA analysis and field sampling surveys do not necessarily match at a finer scale, and suggested the importance of knowledge accumulation for eDNA analysis for proper use of this analysis method to conserve endangered species.

  The Ecological Society of Japan, 2018, Japanese Journal of Conservation Ecology, 23(2) (2), 257 - 264, Japanese

  [Refereed]

  Scientific journal


• Effect of different personal histories on valuation for forest ecosystem services in urban areas: A case study of Mt. Rokko, Kobe, Japan

  Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto

  Dec. 2017, URBAN FORESTRY & URBAN GREENING, 28, 110 - 117, English

  [Refereed]

  Scientific journal


• Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan

  Masayuki K. Sakata, Nobutaka Maki, Hideki Sugiyama, Toshifumi Minamoto

  Dec. 2017, SCIENCE OF NATURE, 104(11-12) (11-12), 100, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA

  Toshiaki Jo, Hiroaki Murakami, Reiji Masuda, Masayuki K. Sakata, Satoshi Yamamoto, Toshifumi Minamoto

  Nov. 2017, MOLECULAR ECOLOGY RESOURCES, 17(6) (6), e25 - e33, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Water sampling for environmental DNA surveys by using an unmanned aerial vehicle

  Hideyuki Doi, Yoshihisa Akamatsu, Yutaka Watanabe, Masuji Goto, Ryutei Inui, Izumi Katano, Mariko Nagano, Teruhiko Takahara, Toshifumi Minamoto

  Nov. 2017, LIMNOLOGY AND OCEANOGRAPHY-METHODS, 15(11) (11), 939 - 944, English

  [Refereed]

  Scientific journal


• Distinct seasonal migration patterns of Japanese native and non-native genotypes of common carp estimated by environmental DNA

  Kimiko Uchii, Hideyuki Doi, Hiroki Yamanaka, Toshifumi Minamoto

  Oct. 2017, ECOLOGY AND EVOLUTION, 7(20) (20), 8515 - 8522, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA method for estimating salamander distribution in headwater streams, and a comparison of water sampling methods

  Izumi Katano, Ken Harada, Hideyuki Doi, Rio Souma, Toshifumi Minamoto

  May 2017, PLOS ONE, 12(5) (5), e0176541 - e0176541, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples

  Hiroki Hashizume, Megumi Sato, Marcello Otake Sato, Sumire Ikeda, Tippayarat Yoonuan, Surapol Sanguankiat, Tiengkham Pongvongsa, Kazuhiko Moji, Toshifumi Minamoto

  May 2017, ACTA TROPICA, 169, 1 - 7, English

  [Refereed]

  Scientific journal


• Water temperature-dependent degradation of environmental DNA and its relation to bacterial abundance

  Satsuki Tsuji, Masayuki Ushio, Sho Sakurai, Toshifumi Minamoto, Hiroki Yamanaka

  Apr. 2017, PLOS ONE, 12(4) (4), e0176608, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant

  Hiroki Yamanaka, Toshifumi Minamoto, Junichi Matsuura, Sho Sakurai, Satsuki Tsuji, Hiromu Motozawa, Masamichi Hongo, Yuki Sogo, Naoki Kakimi, Iori Teramura, Masaki Sugita, Miki Baba, Akihiro Kondo

  Apr. 2017, LIMNOLOGY, 18(2) (2), 233 - 241, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio

  Toshifumi Minamoto, Kimiko Uchii, Teruhiko Takahara, Takumi Kitayoshi, Satsuki Tsuji, Hiroki Yamanaka, Hideyuki Doi

  Mar. 2017, MOLECULAR ECOLOGY RESOURCES, 17(2) (2), 324 - 333, English

  [Refereed]

  Scientific journal


• Isopropanol precipitation method for collecting fish environmental DNA

  Hideyuki Doi, Kimiko Uchii, Saeko Matsuhashi, Teruhiko Takahara, Hiroki Yamanaka, Toshifumi Minamoto

  Feb. 2017, LIMNOLOGY AND OCEANOGRAPHY-METHODS, 15(2) (2), 212 - 218, English

  [Refereed]

  Scientific journal


• Environmental DNA reflects spatial and temporal jellyfish distribution

  Toshifumi Minamoto, Miho Fukuda, Koki R. Katsuhara, Ayaka Fujiwara, Shunsuke Hidaka, Satoshi Yamamoto, Kohji Takahashi, Reiji Masuda

  Feb. 2017, PLOS ONE, 12(2) (2), e0173073, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Random mutagenesis by error-prone polymerase chain reaction using a heavy water solvent

  Toshifumi Minamoto

  Humana Press Inc., 2017, Methods in Molecular Biology, 1498, 491 - 495, English

  [Refereed]

  International conference proceedings


• Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea

  Satoshi Yamamoto, Reiji Masuda, Yukuto Sato, Tetsuya Sado, Hitoshi Araki, Michio Kondoh, Toshifumi Minamoto, Masaki Miya

  Jan. 2017, SCIENTIFIC REPORTS, 7, 40368, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA analysis for estimating the abundance and biomass of stream fish

  Hideyuki Doi, Ryutei Inui, Yoshihisa Akamatsu, Kazuki Kanno, Hiroki Yamanaka, Teruhiko Takahara, Toshifumi Minamoto

  Jan. 2017, FRESHWATER BIOLOGY, 62(1) (1), 30 - 39, English

  [Refereed]

  Scientific journal


• Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples

  Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  Jan. 2017, LIMNOLOGY, 18(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal


• Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

  Masaki Miya, Toshifumi Minamoto, Hiroki Yamanaka, Shin-ichiro Oka, Keiichi Sato, Satoshi Yamamoto, Tetsuya Sado, Hideyuki Doi

  Nov. 2016, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 117(117) (117), e54741, English

  [Refereed][Invited]

  Scientific journal


• On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation

  Hiroki Yamanaka, Hiromu Motozawa, Satsuki Tsuji, Ryohei C. Miyazawa, Teruhiko Takahara, Toshifumi Minamoto

  Nov. 2016, ECOLOGICAL RESEARCH, 31(6) (6), 963 - 967, English

  [Refereed]

  Scientific journal


• Use of environmental DNA to survey the distribution of an invasive submerged plant in ponds

  Ayaka Fujiwara, Saeko Matsuhashi, Hideyuki Doi, Satoshi Yamamoto, Toshifumi Minamoto

  Jun. 2016, FRESHWATER SCIENCE, 35(2) (2), 748 - 754, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Evaluation of the Environmental DNA Method for Estimating Distribution and Biomass of Submerged Aquatic Plants

  Saeko Matsuhashi, Hideyuki Doi, Ayaka Fujiwara, Sonoko Watanabe, Toshifumi Minamoto

  Jun. 2016, PLOS ONE, 11(6) (6), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Supporting citizens' activity scrutinizing scientific information: citizens' seminar on IPCC reports

  EBINA Kuniyoshi, ITOH Masayuki, UMEMURA Kaito, MINAMOTO Toshifumi

  平成27年度日本科学教育学会第7回研究会（四国支部開催); [日時] 平成28年5月28日（土）; [会場]香川大学; [主催]一般社団法人日本科学教育学会

  Japanese Society for Science Education, May 2016, 日本科学教育学会研究会研究報告, 30(7) (7), 17 - 20, Japanese

  Symposium

  Link to Kobe Univ. Repository (Kernel)


• Supporting research activities of high school students using environmental DNA analysis

  MINAMOTO Toshifumi, ITOH Masayuki, EBINA Kuniyoshi

  平成27年度日本科学教育学会第7回研究会（四国支部開催); [日時] 平成28年5月28日（土）; [会場]香川大学; [主催]一般社団法人日本科学教育学会

  Japanese Society for Science Education, May 2016, 日本科学教育学会研究会研究報告, 30(2) (2), 21 - 24, Japanese

  Symposium

  Link to Kobe Univ. Repository (Kernel)


• A novel environmental DNA approach to quantify the cryptic invasion of non-native genotypes

  Kimiko Uchii, Hideyuki Doi, Toshifumi Minamoto

  Mar. 2016, MOLECULAR ECOLOGY RESOURCES, 16(2) (2), 415 - 422, English

  [Refereed]

  Scientific journal


• Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan

  Satoshi Yamamoto, Kenji Minami, Keiichi Fukaya, Kohji Takahashi, Hideki Sawada, Hiroaki Murakami, Satsuki Tsuji, Hiroki Hashizume, Shou Kubonaga, Tomoya Horiuchi, Masamichi Hongo, Jo Nishida, Yuta Okugawa, Ayaka Fujiwara, Miho Fukuda, Shunsuke Hidaka, Keita W. Suzuki, Masaki Miya, Hitoshi Araki, Hiroki Yamanaka, Atsushi Maruyama, Kazushi Miyashita, Reiji Masuda, Toshifumi Minamoto, Michio Kondoh

  Mar. 2016, PLOS ONE, 11(3) (3), e1249786, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The use of environmental DNA of fishes as an efficient method of determining habitat connectivity

  Hiroki Yamanaka, Toshifumi Minamoto

  Mar. 2016, ECOLOGICAL INDICATORS, 62, 147 - 153, English

  [Refereed]

  Scientific journal


• Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction

  Toshifumi Minamoto, Takafumi Naka, Kazuhiko Moji, Atsushi Maruyama

  Jan. 2016, LIMNOLOGY, 17(1) (1), 23 - 32, English

  [Refereed]

  Scientific journal


• Coastal Fish Monitoring Using Environmental DNA

  Minamoto Toshifumi, Yamamoto Satoshi, Kasai Akihide, Kondoh Michio

  Distribution survey methods of macro-organisms using environmental DNA（eDNA）have been rapidly developing in recent years. Environmental DNA analysis can be divided into two major methods, species-specific detection and eDNA metabarcoding. Here we outline these techniques, and we report the preliminary results of an eDNA-based biomass survey of Japanese jack mackere（l Trachurus japonicus）. The results showed significantly positive associations between eDNA concentrations from water samples and echo intensity detected by an echo sounder, suggesting that the spatial variation of the eDNA concentration can reflect the local biomass of fish even in marine environments. More detailed distribut ion mapping of coastal fish can be achieved by environmental DNA analysis, and it will contribute in various fields, such as fisheries science, ichthyology, and ecology.

  Coastal Oceanography Research Committee, the Oceanographic Society of Japan, 2016, Bulletin on Coastal Oceanography, 53(2) (2), 173 - 178, Japanese

  [Refereed][Invited]

  Scientific journal


• Establishment of detection system for native rare species, Hemigrammocypris rasborella, using environmental DNA

  Arisa Fukuoka, Teruhiko Takahara, Munehiro Matsumoto, Biology Club of Hyogo Prefectural Agricultural High School, Atushi Ushimaru, Toshifumi Minamoto

  Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 613 - 620, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA analysis in field research

  Hiroki Yamanaka, Toshifumi Minamoto, Teruhiko Takahara, Kimiko Uchii, Hideyuki Doi

  Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 601 - 612, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Current state of biomonitoring method using environment DNA analysis

  Teruhiko Takahara, Hiroki Yamanaka, Toshifumi Minamoto, Hideyuki Doi, Kimiko Uchii

  Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 583 - 600, Japanese

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection of periodic patterns in microarray data reveals novel oscillating transcripts of biological rhythms in Ciona intestinalis

  Hiromi Matsumae, Ryosuke Ishiwata, Toshifumi Minamoto, Norio Ishida, Soichi Ogishima, Hiroshi Tanaka

  Springer Science and Business Media LLC, Dec. 2015, Artificial Life and Robotics, 20(4) (4), 347 - 352, English

  [Refereed]

  Scientific journal


• MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

  Masaki Miya, Yukuto Sato, Tsukasa Fukunaga, Tetsuya Sado, Keiichi Sato, Toshifumi Minamoto, Satoshi Yamamoto, Hiroaki Yamanaka, Hitoshi Araki, Michio Kondoh, Wataru Iwasaki

  Jul. 2015, ROYAL SOCIETY OPEN SCIENCE, 2(7) (7), 150088, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Preliminary experiment for detection method of fish inhabiting agricultural drainage canals using environmental DNA

  KOIZUMI Noriyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi, DOI Hideyuki, MORI Atsushi, WATANABE Keiji, TAKEMURA Takeshi

  The Japanese Society of Irrigation, Drainage and Rural Engineering, Jun. 2015, Irrigation, Drainage and Rural Engineering Journal, 297(83-3) (83-3), IV_7 - IV_8, English

  [Refereed]


• Droplet Digital Polymerase Chain Reaction (PCR) Outperforms Real-Time PCR in the Detection of Environmental DNA from an Invasive Fish Species

  Hideyuki Doi, Teruhiko Takahara, Toshifumi Minamoto, Saeko Matsuhashi, Kimiko Uchii, Hiroki Yamanaka

  May 2015, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 49(9) (9), 5601 - 5608, English

  [Refereed]

  Scientific journal


• A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan

  Sou Fukumoto, Atushi Ushimaru, Toshifumi Minamoto

  Apr. 2015, JOURNAL OF APPLIED ECOLOGY, 52(2) (2), 358 - 365, English

  [Refereed]

  Scientific journal


• Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys

  Hideyuki Doi, Kimiko Uchii, Teruhiko Takahara, Saeko Matsuhashi, Hiroki Yamanaka, Toshifumi Minamoto

  Mar. 2015, PLOS ONE, 10(3) (3), e0122763 - e0122763, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of sample processing on the detection rate of environmental DNA from the Common Carp (Cyprinus carpio)

  Teruhiko Takahara, Toshifumi Minamoto, Hideyuki Doi

  Mar. 2015, BIOLOGICAL CONSERVATION, 183, 64 - 69, English

  [Refereed]

  Scientific journal


• Monitoring fish pathogenic viruses in natural lakes in Yunnan, China

  Toshifumi Minamoto, Xiaoying Pu, Jie Xie, Yi Dong, Deyi Wu, Hainan Kong, Xiaoxia Yang, Teruhiko Takahara, Mie N. Honjo, Hiroki Yamanaka, Zen'ichiro Kawabata

  Jan. 2015, LIMNOLOGY, 16(1) (1), 69 - 77, English

  [Refereed]

  Scientific journal


• The Release Rate of Environmental DNA from Juvenile and Adult Fish

  Atsushi Maruyama, Keisuke Nakamura, Hiroki Yamanaka, Michio Kondoh, Toshifumi Minamoto

  Dec. 2014, PLOS ONE, 9(12) (12), e114639, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of daily temperature fluctuation on the survival of carp infected with Cyprinid herpesvirus 3

  Teruhiko Takahara, Mie N. Honjo, Kimiko Uchii, Toshifumi Minamoto, Hideyuki Doi, Takafumi Ito, Zen'ichiro Kawabata

  Sep. 2014, AQUACULTURE, 433, 208 - 213, English

  [Refereed]

  Scientific journal


• パブリックコメント・ワークショップの試行 〜「宇宙基本計画（案）」をテーマとしたワークショップの事例報告〜

  MIZUMACHI Eri, KANO Kei, ITOH Masayuki, MINAMOTO Toshifumi, NAKAYAMA Akie, EBINA Kuniyoshi, AKIYA Naonori

  北海道大学高等教育推進機構 高等教育研究部 科学技術コミュニケーション教育研究部門（CoSTEP）, Jun. 2014, Japanese Journal of Science Communication, 15(15) (15), 123 - 136, Japanese

  [Refereed]

  Scientific journal


• Differences between domesticated Eurasian and Japanese indigenous strains of the common carp (Cyprinus carpio) in cortisol release following acute stress

  Teruhiko Takahara, Toshifumi Minamoto, Hideyuki Doi, Takafumi Ito, Zen'ichiro Kawabata

  Apr. 2014, ICHTHYOLOGICAL RESEARCH, 61(2) (2), 165 - 168, English

  [Refereed]

  Scientific journal


• Seasonal reactivation enables Cyprinid herpesvirus 3 to persist in a wild host population

  Kimiko Uchii, Toshifumi Minamoto, Mie N. Honjo, Zen'ichiro Kawabata

  Feb. 2014, FEMS MICROBIOLOGY ECOLOGY, 87(2) (2), 536 - 542, English

  [Refereed]

  Scientific journal


• An emerging infectious pathogen endangers an ancient lineage of common carp by acting synergistically with conspecific exotic strains

  K. Uchii, N. Okuda, T. Minamoto, Z. Kawabata

  Wiley, Jun. 2013, Animal Conservation, 16(3) (3), 324 - 330, English

  [Refereed]

  Scientific journal


• Relationship between aquatic vegetation and water quality in the littoral zones of Lake Dian-chi and Lake Er-hai

  Xie J, Wu D, Chen X, Kong H, Pu X, Yang X, Minamoto T, Yamanaka H, Honjo M. N, Kawabata Z, Li M

  Apr. 2013, Environmental Science & Technology (Wuhan), 36(2) (2), 61 - 65, Chinese

  [Refereed]

  Scientific journal


• Using Environmental DNA to Estimate the Distribution of an Invasive Fish Species in Ponds

  Teruhiko Takahara, Toshifumi Minamoto, Hideyuki Doi

  Feb. 2013, PLOS ONE, 8(2) (2), e56584, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Surveillance of fish species composition using environmental DNA

  Toshifumi Minamoto, Hiroki Yamanaka, Teruhiko Takahara, Mie N. Honjo, Zen'ichiro Kawabata

  Aug. 2012, LIMNOLOGY, 13(2) (2), 193 - 197, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Nationwide Cyprinid herpesvirus 3 contamination in natural rivers of Japan

  Toshifumi Minamoto, Mie N. Honjo, Hiroki Yamanaka, Kimiko Uchii, Zen'ichiro Kawabata

  Aug. 2012, RESEARCH IN VETERINARY SCIENCE, 93(1) (1), 508 - 514, English

  [Refereed]

  Scientific journal


• Estimation of Fish Biomass Using Environmental DNA

  Teruhiko Takahara, Toshifumi Minamoto, Hiroki Yamanaka, Hideyuki Doi, Zen'ichiro Kawabata

  Apr. 2012, PLOS ONE, 7(4) (4), e35868, English

  [Refereed]

  Scientific journal


• Reservoirs of Cyprinid herpesvirus 3 (CyHV-3) DNA in sediments of natural lakes and ponds

  Mie N. Honjo, Toshifumi Minamoto, Zen'ichiro Kawabata

  Mar. 2012, VETERINARY MICROBIOLOGY, 155(2-4) (2-4), 183 - 190, English

  [Refereed]

  Scientific journal


• A new method for random mutagenesis by error-prone polymerase chain reaction using heavy water

  Toshifumi Minamoto, Eitaro Wada, Isamu Shimizu

  Jan. 2012, JOURNAL OF BIOTECHNOLOGY, 157(1) (1), 71 - 74, English

  [Refereed]

  Scientific journal


• Expression of spliced variants of period mRNA in the Japanese honeybee Apis cerana japonica

  Toshifumi Minamoto, Taro Fuchikawa, Isamu Shimizu

  2012, BIOLOGICAL RHYTHM RESEARCH, 43(2) (2), 125 - 135, English

  [Refereed]

  Scientific journal


• Spatial-temporal analysis of water temperatures during spring in Lake Erhai, China: implications for fisheries

  Hiroki Yamanaka, Toshifumi Minamoto, Deyi Wu, Hainan Kong, Zhihong Wei, Bin Liu, Zen'ichiro Kawabata

  2012, INLAND WATERS, 2(3) (3), 129 - 136, English

  [Refereed]

  Scientific journal


• Environment-KHV-carp-human linkage as a model for environmental diseases

  Zen'ichiro Kawabata, Toshifumi Minamoto, Mie N. Honjo, Kimiko Uchii, Hiroki Yamanaka, Alata A. Suzuki, Yukihiro Kohmatsu, Kota Asano, Tomoaki Itayama, Tomoaki Ichijo, Koji Omori, Noboru Okuda, Masayuki Kakehashi, Masao Nasu, Kazuaki Matsui, Masatomi Matsuoka, Hainan Kong, Teruhiko Takahara, Deyi Wu, Ryuji Yonekura

  Nov. 2011, ECOLOGICAL RESEARCH, 26(6) (6), 1011 - 1016, English

  [Refereed]

  Scientific journal


• Stress response to daily temperature fluctuations in common carp, Cyprinus carpio L.

  Teruhiko Takahara, Hiroki Yamanaka, Alata A. Suzuki, Mie N. Honjo, Toshifumi Minamoto, Ryuji Yonekura, Tomoaki Itayama, Yukihiro Kohmatsu, Takafumi Ito, Zen'ichiro Kawabata

  Oct. 2011, HYDROBIOLOGIA, 675(1) (1), 65 - 73, English

  [Refereed]

  Scientific journal


• Detection of cyprinid herpesvirus-3 DNA in lake plankton

  Toshifumi Minamoto, Mie N. Honjo, Hiroki Yamanaka, Nobuyuki Tanaka, Tomoaki Itayama, Zen'ichiro Kawabata

  Lead, Jun. 2011, RESEARCH IN VETERINARY SCIENCE, 90(3) (3), 530 - 532, English

  [Refereed]

  Scientific journal


• Transmission dynamics of an emerging infectious disease in wildlife through host reproductive cycles

  Kimiko Uchii, Arndt Telschow, Toshifumi Minamoto, Hiroki Yamanaka, Mie N. Honjo, Kazuaki Matsui, Zen'ichiro Kawabata

  Feb. 2011, ISME JOURNAL, 5(2) (2), 244 - 251, English

  [Refereed]

  Scientific journal


• Apis cerana japonica Discriminates between Floral Color Phases of the Oriental Orchid, Cymbidium floribundum

  Michio Sugahara, Toshifumi Minamoto, Taro Fuchikawa, Masanao Michinomae, Isamu Shimizu

  Dec. 2010, ZOOLOGICAL SCIENCE, 27(12) (12), 901 - 906, English

  [Refereed]

  Scientific journal


• Spatial variation and temporal stability of littoral water temperature relative to lakeshore morphometry: environmental analysis from the view of fish thermal ecology

  Hiroki Yamanaka, Yukihiro Kohmatsu, Toshifumi Minamoto, Zen'ichiro Kawabata

  Apr. 2010, LIMNOLOGY, 11(1) (1), 71 - 76, English

  [Refereed]

  Scientific journal


• Circadian clock in Ciona intestinalis revealed by microarray analysis and oxygen consumption

  Toshifumi Minamoto, Shuji Hanai, Koji Kadota, Katsutaka Oishi, Hiromi Matsumae, Manabu Fujie, Kaoru Azumi, Noriyuki Satoh, Masanobu Satake, Norio Ishida

  Feb. 2010, JOURNAL OF BIOCHEMISTRY, 147(2) (2), 175 - 184, English

  [Refereed]

  Scientific journal


• Quantification of Cyprinid Herpesvirus 3 in Environmental Water by Using an External Standard Virus

  Mie N. Honjo, Toshifumi Minamoto, Kazuaki Matsui, Kimiko Uchii, Hiroki Yamanaka, Alata A. Suzuki, Yukihiro Kohmatsu, Takaji Iida, Zen'ichiro Kawabata

  Jan. 2010, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(1) (1), 161 - 168, English

  [Refereed]

  Scientific journal


• Seasonal Distribution of Cyprinid Herpesvirus 3 in Lake Biwa, Japan

  Toshifumi Minamoto, Mie N. Honjo, Zen'ichiro Kawabata

  Nov. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(21) (21), 6900 - 6904, English

  [Refereed]

  Scientific journal


• Detection of cyprinid herpesvirus 3 DNA in river water during and after an outbreak

  Toshifumi Minamoto, Mie N. Honjo, Kimiko Uchii, Hiroki Yamanaka, Alata A. Suzuki, Yukihiro Kohmatsu, Takaji Iida, Zen'ichiro Kawabata

  Mar. 2009, VETERINARY MICROBIOLOGY, 135(3-4) (3-4), 261 - 266, English

  [Refereed]

  Scientific journal


• 大阪府下のミツバチ生息状態

  菅原道夫, 清水良訓, 源利文, 清水勇

  玉川大学ミツバチ科学研究所, Dec. 2006, ミツバチ科学, 27(1) (1), 19 - 22, Japanese

  Research institution


• Molecular mechanism of visual adaptation in fish

  Minamoto T, Shomizu I

  日本魚類学会, Nov. 2005, Japan. J. Ichthyol., 52(2) (2), 91 - 106, Japanese

  [Refereed]

  Scientific journal


• Molecular cloning of cone opsin genes and their expression in the retina of a smelt, Ayu (Plecoglossus altivelis, Teleostei)

  Toshifumi Minamoto, Isamu Shimizu

  Lead, 2, Feb. 2005, Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 140(2) (2), 197 - 205, English

  [Refereed]

  Scientific journal


• キンリョウヘンCymbidium floribundum唇弁の着色がニホンミツバチApis cerana japonicaの訪花行動にあたえる影響

  菅原道夫, 源利文, 清水勇, 東克

  玉川大学ミツバチ科学研究所, Nov. 2003, ミツバチ科学, 24(3) (3), 115 - 118, Japanese

  Research institution


• Molecular cloning and characterization of rhodopsin in a teleost (Plecoglossus altivelis, Osmeridae)

  Toshifumi Minamoto, Isamu Shimizu

  Lead, Apr. 2003, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 134(4) (4), 559 - 570, English

  [Refereed]

  Scientific journal


• Molecular cloning of lysozyme-encoding cDNAs expressed in the salivary gland of a wood-feeding termite, Reticulitermes speratus

  Ai Fujita, Toshifumi Minamoto, Isamu Shimizu, Takuya Abe

  Dec. 2002, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 32(12) (12), 1615 - 1624, English

  [Refereed]

  Scientific journal


• Studies of opsin genes in a smelt fish, Ayu (Plecoglossus altivelis)

  Toshifumi Minamoto, Isamu Shimizu

  Lead, Korean Society of PhotoScience, 2002, Journal of Photoscience, 9(2) (2), 269 - 271, English

  International conference proceedings


• Phylogenetic relationship among osmerid and salangid fish inferred from mitochondrial cytochrome b gene sequences

  Toshifumi Minamoto, Isamu Shimizu

  Lead, Faculty of Science, Kyoto University, 2002, Memoirs of the Faculty of Science, Kyoto University (Series of Biology), 18, 1 - 13, English

  Research institution


• A novel isoform of vertebrate ancient opsin in a smelt fish, Plecoglossus altivelis

  Toshifumi Minamoto, Isamu Shimizu

  Lead, Jan. 2002, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 290(1) (1), 280 - 286, English

  [Refereed]

  Scientific journal


• Cloning of gene encoding the visual pigments in the silkworm, Bombyx mori

  Isamu Shimizu, Yoshinori Yamakawa, Toshifumi Minamoto, Katsuhiko Sakamoto

  Feb. 1998, APPLIED ENTOMOLOGY AND ZOOLOGY, 33(1) (1), 199 - 204, English

  [Refereed]

  Scientific journal

• 環境DNA調査の広がりと今後の期待

  源利文

  Lead, Jan. 2025, ミルシル, 18(1) (1), 15 - 17, Japanese

  [Invited]

  Introduction other


• 教科書的には正しくないことがおもしろい

  源利文

  Lead, Dec. 2024, 中等教育資料, (1063) (1063), 2 - 3, Japanese

  [Invited]

  Introduction other


• The eDNA Society International Meeting 2023, “Moving from knowledge into practice”

  Hiroki Yamanaka, Hideyuki Doi, Hitoshi Araki, Kimiko Uchii, Toshifumi Minamoto

  Abstract With the aim to gather and synthesize knowledge regarding environmental DNA analysis and its applications, The eDNA Society International Meeting 2023 was held in Otsu, Shiga, Japan, from May 17th to 19th with more than 252 participants from 19 countries. During the three core days, there were three plenary sessions with nine leading researchers in the field as invited speakers, as well as general oral sessions, a poster session, workshops presented by the society's corporate companies, and opening and closing ceremonies. The meeting had been postponed multiple times due to the COVID‐19 pandemic but was finally held in‐person, with options for online attendance, in front of Lake Biwa. This is a memorial site where the first field test for eDNA analysis in Japan was conducted. Here, we report the record of the meeting by sharing the highlights of the three core days. The proposed meeting theme, “Moving from Knowledge into Practice,” was well achieved during the meeting with energetic participants and enthusiastic discussion, which was stimulated by interesting presentations and lectures spanning from basic technical developments to practical applications.

  Wiley, Nov. 2023, Environmental DNA, 5, 1191 - 1195, English

  Meeting report


• Assessing the distribution of invasive alien ants by environmental DNA analysis of soils (in Japanese)

  Toshifumi Minamoto

  Lead, Apr. 2022, Pest Control, 198, 20 - 25, Japanese

  [Invited]

  Introduction other


• Attempt to obtain information on aquatic organisms using environmental nucleic acids

  MINAMOTO Toshifumi

  Lead, Feb. 2020, JMOA Report, (19) (19), 1 - 10, Japanese

  [Invited]

  Introduction other


• Development and application of environmental DNA analysis for macro-organisms

  MINAMOTO Toshifumi

  Nov. 2019, KAGAKU, 89(11) (11), 1029 - 1035, Japanese

  [Invited]

  Introduction commerce magazine


• Environmental DNA: A novel monitoring tool for aquatic organisms

  MINAMOTO Toshifumi

  Mar. 2019, Seiryu-Seiko, 146, 10 - 11, Japanese

  [Invited]

  Introduction other


• Outline of environmental DNA analysis and detection of rare species: distribution of endangered species discovered by a cup of water

  MINAMOTO Toshifumi

  Mar. 2019, Kagaku to Seibutsu (Chemistry and Organisms), 57(3) (3), 181 - 186, Japanese

  [Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 環境DNA技術の現状と今後の展望(座談会)—特集 環境DNAを考える

  源 利文, 乾 隆帝, 大井 和之, 藤井 暁彦

  九州環境管理協会, 2019, 環境管理 = Environmental evaluation, (48) (48), 4 - 14, Japanese


• Ecological surveys of macro-organisms based on species-specic detection of environmental DNA

  MINAMOTO Toshifumi

  Apr. 2018, Journal of Japan Society on Water Environment, 41A(4) (4), 123 - 127, Japanese

  [Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Environmental DNA: A new tool for biological monitoring with a bottle of water

  IKEDA Kousuke, DOI Hideyuki, MINAMOTO Toshifumi

  The Institution of Professional Engineers, Japan, Mar. 2018, IPEJ Journal, 30(3) (3), 20 - 21, Japanese

  Introduction scientific journal


• Evaluating the efficiency of environmental DNA through tnak experiments and field surveys

  MASUDA Reiji, MURAKAMI Hiroaki, TAKAHASHI Kohji, MINAMOTO Toshifumi, MIYA Masaki

  Feb. 2018, Aquabiology, 40(1) (1), 17 - 22, Japanese

  [Invited]

  Introduction commerce magazine


• The potential of environmental DNA for the estimation of population abundance and biomass

  FUKAYA Keiichi, OSADA Yutaka, MINAMOTO TOSHIFUMI

  Feb. 2018, Aquabiology, 40(1) (1), 47 - 53, Japanese

  [Invited]

  Introduction commerce magazine


• What is environmental DNA?

  MINAMOTO Toshifumi

  Feb. 2018, Aquabiology, 40(1) (1), 3 - 8, Japanese

  [Invited]

  Introduction commerce magazine


• Environmental DNA and its application in the detection and control of fish and water borne zoonosis: Opisthorchiasis in LAOs and schistosomiasis in Madagascar

  Marcello Otake Sato, Armand Rafalimanantsoa Solofoniaina, Megumi Sato, Tiengkham Pongvongsa, Toshifumi Minamoto, Satoru Kawai, Yuichi Chigusa

  Dokkyo University School of Medicine, 2018, Dokkyo Journal of Medical Sciences, 45(2) (2), 85, English

  Report scientific journal


• Current developmental status of environmental DNA monitoring for aquatic environments

  MINAMOTO Toshifumi

  Dec. 2017, Environmental Conservation Engineering, 46(12) (12), 624 - 629, Japanese

  [Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Issues and prospects of environmental DNA monitoring methods

  MINAMOTO Toshifumi, UCHII Kimiko, TAKAHARA Teruhiko, DOI Hideyuki

  Dec. 2017, Environmental Conservation Engineering, 46(12) (12), 648 - 652, Japanese

  [Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 大量シーケンスと標準DNAを利用した魚類環境DNAの網羅的・定量的モニタリング:京都府舞鶴湾での解析事例

  潮雅之, 潮雅之, 村上弘章, 益田玲爾, 佐土哲也, 宮正樹, 櫻井翔, 山中裕樹, 源利文, 近藤倫生

  10 Nov. 2017, 水産海洋研究, 81(4) (4), 305‐307, Japanese


• Biological survey by high school students using environmental DNA analysis

  MINAMOTO Toshifumi

  Jun. 2017, Education Study Group for Biology of High School in Hyogo Prefecture, 41, 2 - 5, Japanese

  [Invited]

  Introduction other


• Expectation for infectious disease studies using environmental DNA

  MINAMOTO Toshifumi

  Environmental DNA analysis for micro- and macro-organisms is rapidly developing. Environmental DNA means total DNA present in environmental media such as water or soil, and includes DNA contained in the organisms themselves and extra-organism DNA of macro-organisms. Analysis of environmental DNA can be divided into two methods, species-specific detection and meta-barcoding, which can be used according to each purpose. Applicable subjects are all organisms (including viruses in this case) with DNA as genes, and application to rivers, ponds, lakes and marines has been reported. In this paper, the present situation of environmental DNA analysis of macro organisms is described, and the possibility of application to infectious disease studies and the problems to be solved are discussed.

  The Japanese Society for Virology, Jan. 2017, Virus, 66(2) (2), 171 - 178, Japanese

  [Invited]

  Introduction scientific journal


• Aquatic species census using environmental DNA

  MINAMOTO Toshifumi

  CMC Publishing, Jun. 2016, BIOINDUSTRY, 33(6) (6), 60 - 65, Japanese

  [Invited]

  Introduction commerce magazine


• 環境DNAメタバーコーディングを用いた淀川の魚類相モニタリング

  稲波璃香, 山本義彦, 近藤美麻, 上原一彦, 佐藤行人, 宮正樹, 山本哲史, 源利文

  2016, 日本生態学会大会講演要旨(Web), 63rd


• Impact of environmental DNA analysis

  IWASAKI Wataru, SATO Yukuto, MINAMOTO Toshifumi, YAMANAKA Hiroki, ARAKI Hitoshi, MIYA Masaki

  Jan. 2016, Jikken Igaku (Experimental Medicine), 34(1) (1), 103 - 107, Japanese

  [Invited]

  Introduction commerce magazine


• Environmental DNA analysis: A new monitoring tool for aquatic species

  Kimiko Uchii, Toshifumi Minamoto, Hideyuki Doi, Teruhiko Takahara, Hiroki Yamanaka, Izumi Katano

  Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 581 - 582, Japanese

  Report scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Towards the further developments of environmental DNA analysis

  Toshifumi Minamoto, Kimiko Uchii, Hiroki Yamanaka, Teruhiko Takahara, Izumi Katano, Hideyuki Doi

  Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 621 - 624, Japanese

  Report scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The Release Rate of Environmental DNA from Juvenile and Adult Fish (vol 9, e114639, 2014)

  A. Maruyama, K. Nakamura, H. Yamanaka, M. Kondoh, T. Minamoto

  Mar. 2015, PLOS ONE, 10(3) (3), English

  Others


• Environmental DNA: A novel tool for Ecohealth research

  MINAMOTO Toshifumi

  Ishiyaku Publishers, Icn., Jun. 2014, Journal of Clinical and Experimental Medicine, 249(12) (12), 1264 - 1269, Japanese

  [Invited]

  Introduction commerce magazine


• ANALYSIS OF mRNA VARIANTS OF PERIOD GENES IN APIS CERANA AND OTHER HYMENOPTERA INSECTS(Physiology&Biochemistry,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan) :

  Minamoto Toshifumi, Shimizu Isamu

  Zoological Society of Japan, 2003, Zoological science, 20(12) (12), 1593 - 1593, English

• 環境DNA入門 ただよう遺伝子は何を語るか

  源利文

  Single work, 岩波書店, 2022, Japanese, ISBN: 9784000297158

  General book


• Cutting-edge Analytical Techniques, 2nd Edition

  Toshifumi Minamoto

  Contributor, pp. 756-765. "Environmental DNA analysis", NTS Inc., Jan. 2022, Japanese

  Dictionary or encycropedia


• Environmental DNA: Deciphering the True Nature of Ecosystems (Ed. by H. Doi and M. Kondoh) (in Japanese)

  Toshifumi Minamoto

  Joint work, pp. 249-253. column "Standardization of Methods in Environmental DNA Analysis", Kyoritsu Syuppan Co. LTD., Mar. 2021


• Environmental DNA: Deciphering the True Nature of Ecosystems (Ed. by H. Doi and M. Kondoh) (in Japanese)

  Masayuki K. Sakata, Toshiaki Jo, Toshifumi Minamoto

  Contributor, pp. 1-35. Chapter 1 "Overview of Environmental DNA Analysis", Kyoritsu Syuppan Co. LTD., Mar. 2021


• The Encyclopedia of Ichthyology (in Japanese)

  MINAMOTO Toshifumi

  Contributor, pp. 492-493, "Environmental DNA", Maruzen Publishing, Oct. 2018, Japanese

  Dictionary or encycropedia


• In Vitro Mutagenesis Methods and Protocols (Methods in Molecular Biology vol. 1498; Ed. Andrew Reeves)

  Toshifumi Minamoto

  Contributor, pp.491-495. Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent, Springer New York, Oct. 2016, English, ISBN: 9781493964703

  Scholarly book


• Pathogenic mechanism of infectious diseases. In Ecology of Infectiuos diseases (Ecological Society of Japan ed.), Kyoritsu Syuppan (Tokyo),

  MINAMOTO Toshifumi

  Contributor, pp. 39-51., Kyoritsu Syuppan, Mar. 2016, Japanese

  Scholarly book


• 身近な水の環境科学 実習・測定編 （日本陸水学会東海支部会編）

  Teruhiko Takahara, Hideyuki Doi, Toshifumi Minamoto

  Contributor, pp. 159-161. コラム「水を調べるだけで生き物がわかる！環境DNAを利用した生物分布モニタリング法」, 朝倉書店, Jun. 2014, Japanese

  Textbook


• Our Lakes: From the Present towards a Future Perspective (RIHN-China Study Series No.3: Kawabata Z., Kong H., Wu D., FUKUSHI Y., Kubota J. eds)

  MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  Contributor, pp. 75-86. "Environmental Change of the Lake and Infectious Disease", 松香堂書店, Mar. 2014, Japanese

  Scholarly book


• Our Lakes: From the Present towards a Future Perspective (RIHN-China Study Series No.3: Kawabata Z., Kong H., Wu D., FUKUSHI Y., Kubota J. eds)

  XIE Qing, WU Deyi, MINAMOTO Toshifumi, YAMANAKA Hiroki, HONJO Mie N, KAWABATA Zen'ichiro

  Contributor, pp. 139-151. "An On-Site Empirical Study on the Role of Aquatic Plants in Improving Lakefromt Water Quality", 松香堂書店, Mar. 2014, Chinese

  Scholarly book


• 地球環境学マニュアル２ はかる・みせる・読みとく（総合地球環境学研究所編）

  Toshifumi Minamoto

  Contributor, pp. 68-69. 第4章 4.4 「環境DNAを用いた水中動物相のモニタリング」, 総合地球環境学研究所, Jan. 2014, Japanese

  Dictionary or encycropedia


• 生物の多様性ってなんだろう-生命のジグソーパズル (京都大学総合博物館・京都大学生態学研究センター編)

  Isamu Shimizu, Toshifumi Minamoto

  Contributor, pp140-164 魚類の多様性とオプシン遺伝子, 京都大学学術出版会, 2007, Japanese, ISBN: 9784876988273

  General book


• DNAチップ活用テクノロジーと応用 (久原哲編)

  Norio Ishida, Toshifumi Minamoto

  Contributor, pp 108-117「DNAチップを用いた生物時計機能解析－ショウジョウバエの交尾行動リズムとホヤの体内時計」, シーエムシー出版, Sep. 2006, Japanese, ISBN: 9784882315902

  Scholarly book


• 生物多様性のすすめ－生態学からのアプローチ(大串隆之編)

  Isamu Shimizu, Toshifumi Minamoto

  Contributor, pp90-109 「見える世界が魚を変える－魚類の多様性と視覚適応」, 丸善, 2003, Japanese, ISBN: 9784621072189

  General book

• 自然共生サイトにおける環境DNA分析と希少種の保全にむけた小さなアクション

  源利文

  第41回個体群生態学会, Sep. 2025, Japanese

  [Invited]

  Nominated symposium


• Development of a high-sensitivity environmental DNA detection method for eel species using UnaSINE target primers

  Hirayama, I. T., Kunimasa, Y., Takeuchi, A., Minamoto, T.

  1st Asian International Eel Symposium, Sep. 2025, Japanese

  Poster presentation


• ダム湖底におけるエビ類の生息状況と環境DNAによる検出可能性の検討

  稲川崇史, 鄔倩倩, 岡本理央, 大杉奉功, 沖津二朗, 坂本正吾, 東信行, 梅田信, 源利文

  応用生態工学会 第２8回新潟大会, Sep. 2025, Japanese

  Poster presentation


• 高効率eDNA濃縮手法QuickConcの大容量試料への適用

  黒板智博, Qianqian Wu, 岩本遼, 源利文

  応用生態工学会 第２8回新潟大会, Sep. 2025, Japanese

  Poster presentation


• 大量の水試料中のDNA高効率・迅速濃縮手法の開発

  岩本遼, 黒板智博, Qianqian Wu, 源利文

  日本微生物生態学会第38回東京大会, Sep. 2025, Japanese

  Poster presentation


• Recent developments in environmental DNA (eDNA) analysis and its expanding applications in pathogen monitoring

  Toshifumi Minamoto

  The 4th International Conference on Parasitology 2025, Jul. 2025, English

  [Invited]

  Invited oral presentation


• The novel identification method of the hybrid using environmental DNA

  Masayuki K. Sakata, Nanako Yano, Akio Imamura, Hiroki Yamanaka, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• Impact of global warming on Lake Biwa fish: identifying climate-sensitive species

  Qianqian Wu, Jinxin Zhou, Yuan Yao, Toshiyuki Ishikawa, Daisuke Kitazawa, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• Impact of farmland abandonment on survival of a small salamander (Hynobius setouchi) using environmental DNA analysis

  Nana Matsumoto, Masayuki K. Sakata, Yuta Kunimasa, Yuna Yamamoto, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• How to monitor pollinators: Comparison among conventional and eDNA metabarcoding methods

  Eimi Nagahama, Gaku Hirayama, Tomoya Yoshihara, Kazuya Takeda, Toshifumi Minamoto, Atushi Ushimaru

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• Approaches to enhancing the detection sensitivity of Schistosoma mansoni eDNA

  Yuna Yamamoto, Qianqian Wu, Evans Asena Chadeka, Benard Ngetich, Ododa George, Sachiyo Nagi, Kyoko Futami, Ayako Hyuga, Sammy Njenga, Collins Ouma, Shinjiro Hamano, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• Analysis of the relationship between anurans population density and landscape factors in paddy field using environmental DNA analysis

  Akinori Ogura, Qianqian Wu, Ryohei Nakao, Atushi Ushimaru, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• Fish communities monitoring using eDNA metabarcoding considering seasonal and depth variations

  Ryota P. Kitani, Rikuto Yazawa, Towa Masuda, Yo Kurazono, Yuichi Yaoi, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  Poster presentation


• Expanding eDNA applications through epigenetics: spawning monitoring focus

  Itsuki T. Hirayama, Toshifumi Minamoto

  The 11th EAFES International Congress, Jul. 2025, English

  [Invited]

  Nominated symposium


• Monitoring of biological distribution by environmental DNA analysis and its application to epigenetics

  Toshifumi Minamoto

  The 53nd Annual Meeting of the Japanese Society of Toxicology, Jul. 2025, Japanese

  [Invited]

  Nominated symposium


• Cumulative negative effects of river crossing structures on the Japanese eel upstream migration

  Yuta Kunimasa, Nagisa Hashimoto, Ryota P. Kitani, Nakata Taichi, Manabu Kume, Tomoichiro Tanaka, Hiromichi Mitamura, Toshifumi Minamoto

  32nd International Congress for Conservation Biology, Jun. 2025, English

  Poster presentation


• QuickConc: Rapid eDNA concentration and ambient temperature biobanking for enhanced environmental DNA monitoring

  Ryo Iwamoto, Tomohiro Kuroita, Qianqian Wu, Toshifumi Minamoto

  ISBER 2025 Annual Meeting, May 2025, English

  Oral presentation


• Frontline of eDNA studies: Identifying reproductive behavior

  Toshifumi Minamoto

  温州大学 第八十一期生態学前沿大講堂, Apr. 2025, Japanese

  [Invited]

  Public discourse


• 高収率な環境DNA濃縮手法QuickConcの開発

  岩本遼, 黒板智博, 鄔倩倩, 源利文

  令和7年度日本水産学会春季大会, Mar. 2025, Japanese

  Oral presentation


• DNA分析手法を用いたホシガレイ人工種苗の捕食者の探索

  叶一希, 村上弘章, 深野直孝, 恩田都和, 源利文, 鄔倩倩, 山野辺貴寛, 成田薫, 舟木優斗, 小木曽奏斗, 荒井優志, 角野和史, 久米学, 高木淳一, 和田敏裕, 三田村啓理

  令和7年度日本水産学会春季大会, Mar. 2025, Japanese

  Poster presentation


• Enhancing eDNA metabarcoding accuracy: mitigating PCR bias with droplet PCR

  Yamanaka, H., Shaffer, M. R., Scott, O. M., Alla, E. A., Minamoto, T., Kelly, R. P.

  AquaEcOmics 2025, Mar. 2025, English

  Oral presentation


• QuickConc: a novel cationic-assisted eDNA capture method for enhanced biodiversity monitoring

  Iwamoto, R., Kuroita, T., Wu, Q., Minamoto, T.

  AquaEcOmics 2025, Mar. 2025, English

  Oral presentation


• Environmental surveillance of Opisthorchis viverrini in endemic areas of Champasack

  Prasayasith, P., Valencia, J. E., Revolteado, M. J. V., Hansana, P., Pakouakue, B., Matsuo, R., Matsumura, N., Thanabouasy, C., Laymanivong, S., Sayosone, S., Banouvong, V., Buchy, P., Iwagami, M., Sato, M. O., Minamoto, T., Sato, M.

  The 94th Annual Meeting of Japanese Society for Parasitology, Mar. 2025, English

  Oral presentation


• 環境DNA分析を用いた水田におけるカエル類の個体数密度と景観要因の関係の解析

  小倉彰紀, 鄔倩倩, 中尾遼平, 丑丸敦史, 源利文

  第72回日本生態学会大会, Mar. 2025, Japanese

  Poster presentation


• 環境DNAを用いたタイ肝吸虫検出系の開発および感染症生態学への応用

  松尾莉子, Prasayasith, P., Valencia, J. E., Revolteado, M. J. V., Adisakwattana, P., Yoonuan, T., Phuohisut, O., Limpanont, Y., Sato, M. O., Samo, M., Pongvongsa, T., 源利文

  第72回日本生態学会大会, Mar. 2025, Japanese

  Poster presentation


• 温暖化は琵琶湖の魚類の分布にどのような影響を与えるのか？

  鄔倩倩, 周金鑫, 姚遠, 石川俊之, 北澤大輔, 源利文

  第72回日本生態学会大会, Mar. 2025, Japanese

  Poster presentation


• 環境DNAにおける精子特異的メチル化状態を利用したカワバタモロコの繁殖検出

  平山一槻, 源利文

  第72回日本生態学会大会, Mar. 2025, Japanese

  Poster presentation


• Tracking reproductive activities using environmental DNA

  Toshifumi Minamoto

  2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, English

  [Invited]

  Keynote oral presentation


• QuickConc: A rapid, efficient, and power-free eDNA concentration method for diverse aquatic environments

  Ryo Iwamoto, Tomohiro Kuroita, Qianqian Wu, Toshifumi Minamoto

  2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, English

  Oral presentation


• Environmental DNA can detect the hybrid

  Masayuki K. Sakata, Nanako Yamo, Akio Imamura, Hiroki Yamanaka, Toshifumi Minamoto

  2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, English

  Poster presentation


• Negative effects of farmland abandonment on the reproduction of a small salamander (Hynobius setouchi)

  Nana Matsumoto, Masayuki K. Sakata, Toshifumi Minamoto

  2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, English

  Poster presentation


• QuickConc: A rapid, efficient, and power-free eDNA concentration method for diverse aquatic environments

  Masayuki Imai, Tomohiro Kuroita, Ryu Iwamoto, Qianqian Wu, Tshifumi Minamoto

  International Symposium on Environmental DNA for Conservation and Biomonitoring in Southeast Asia 2025 (eDNAConBio 2025), Feb. 2025, English

  Oral presentation


• 環境DNAメタバーコーディング法を活用した新たな樹上生物多様性モニタリング法の開発―樹幹流を利用して―

  坂田歩美, 佐土哲也, 岡慎一郎, 潮雅之, 宮正樹, 源利文, 蘭光健人, 池田裕二, 海老原淳, 井上侑哉, 坪田博美, 原田浩

  日本地衣学会第23回大会, Nov. 2024, Japanese

  Oral presentation


• Development of a rapid, efficient, and power-free eDNA concentration method (QuickConc)

  Tomohiro Kuroita, Qianqian Wu, Ryo Iwamoto, Toshifumi Minamoto

  The 7th Annual Meeeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Attempt to understand crustaceans inhabiting the bottom of a dam reservoir by eDNA analysis

  Takashi Inagawa, Rio Okamoto, Qianqian Wu, Tomonori Osugi, Jiro Okitsu, Shogo Sakamoto, Nobuyuki Azyma, Makoto Umeda, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, Japanese

  Poster presentation


• Potential of droplet PCR to ameliorate biased amplification in library preparation for eDNA metabarcoding

  Hiroki Yamananaka, Megan R. Shaffer, Olivia M. Sco, Elizabeth Andruszkiewicz Allan, Toshifumi Minamoto, Ryan P. Kelly

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, Japanese

  [Invited]

  Nominated symposium


• Environmental DNA can detect a hybrid

  Masayuki K. Sakata, Nanako Yano, Akio Imamura, Hiroki Yamanaka, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, Japanese

  [Invited]

  Nominated symposium


• Influence of homogenization methods on lichen species detection from environmental DNA metabarcoding

  Ayumi Sakata, Toshifumi Minamoto, Tetsuya Sado, Masaki Miya

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, Japanese

  Poster presentation


• Development of a detection tool and distribution analyses for the nuisance alien diatom Cymbella janischii

  Yoko Kato-Unoki, Tamie Suzawa, Trang Tran Thu, Toshifumi Minamoto, Yoshitsugu Masuda, Jun-ichi Tsuboi, Satoquo Seino

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Introducing Epigenetics into Environmental DNA Analysis: Opportunities and Challenges

  Itsuki Hirayama, Luhan Wu, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, Japanese

  [Invited]

  Nominated symposium


• Elucidating the spatiotemporal distribution dynamics of black bass species in Lake Biwa

  Atsushi Okada, Kei Wakimura, Kimiko Uchii, Qianqian Wu, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Estimation of environmental factors regulating the distribution of Setouchi salamanders in abandoned farmland

  Nana Matsumoto, Masayuki K. Sakata, Yuta Kunimasa, Yuna Yamamoto, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Study on the distribution of native and non-native bitterling fish in the Okayama Plain

  Kanta Fujino, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Collection of environmental DNA from stemflow for detecting terrestrial mammals

  Yuya Ishihara, Kazumi Aoki, Ayumi Sakata, Masaki Miya, Kyouhei Hamano, Masayuki K. Sakata, Qianqian Wu, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Development of detection assay for Schistosoma mekongi eDNA and its application in the field

  Nao Matsumura, Riko Matsuo, Megumi Sato Marcello, Otake Sato, Joseph Evangelista Valencia, Mark June Valiente, Revolteado, Phoyphaylinh Prasayasith, Poom Adisakwattana, Yanin Limpanon, Orawan Phuphisu, Tippayarat Yoonuan, Somphou Sayasone, Masayuki K. Sakata, Qianqian Wu, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• Estimation of distribution factors of Opisthorchis viverrini, in Savannakhet province, Lao PDR

  Riko Matsuo, Phoyphaylinh Prasayasith, Joseph Evangelista Valencia, Mark June Valiente Revolteadom, Poom Adisakwattana, Tippayarat Yoonuan, Orawan Phuphisu, Yanin Limpanon, Marcello Otake Sato, Megumi Sato, Tiengkham Pongvongsa, Toshifumi Minamoto

  The 7th Annual Meeting of The eDNA Society, Dec. 2024, English

  Poster presentation


• 迅速、効率的なeDNA核酸濃縮手法(QuickConc)の開発

  岩本遼, 黒板智博, Wu Qianqian, 源利文

  第４７回日本分子生物学会年会, Nov. 2024, Japanese

  Poster presentation


• Monitoring the distribution of hatchery-reared spotted halibut (Verasper variegatus) juveniles after release using environmental DNA

  Murakami, H., Fukano, N., Wu, Q., Minamoto, T., Takagi, J., Kume, M., Yamanobe, T., Yamashita, Y., Mitamura, H., Wada., T.

  The 11th International Flatfish Symposium, Nov. 2024, English

  Oral presentation


• QuickConc: Fast, efficient, power-free eDNA capture

  Ryo Iwamoto, Tomohiro Kuroita, Qianqian Wu, Toshifumi Minamoto

  Biodiversity Genomics Conference 2024, Oct. 2024, Esperanto

  Oral presentation


• Seasonality of Opisthorchis viverrini environmental DNA detection in endemic areas of Champasack, Lao-PDR

  Prasayasith, P., Valencia, J., Revolteado, M. J., Hansana, P., Pakouakue, B., Matsuo, R., Matsumura, N., Thanabouasy, C., Laymanivong, S., Sayasone, S., Banouvong, V., Buchy, P., Iwagami, M., Sato, M. O., Minamoto, T., Sato, M.

  The 14th Lao National Health Research Forum, Oct. 2024, English


• Eco Health/One Health towards eradication of opisthorchiasis using the environmental DNA detection as an evaluation tool

  Sato, M., Pongvongsa, T., Sayasone, S., Prasayasith, P., Valencia, J., Revolteado, M. J., Younuan, T., Adisakwattana, P., Minamoto, T., Sato, M. O.

  The 14th Lao National Health Research Forum, Oct. 2024, English


• Investigating the habitat of Aphelocheirus nawai using environmental DNA analysis

  Tomoharu Hino, Ryota P. Kitani, Yuta Kunimasa, Toshifumi Minamoto

  The 29th Biennial Conference of Asian Association for Biology Education, Oct. 2024, English


• Evaluation of the effectiveness of environmental education using environmental DNA analysis: Is it effective even for people who hesitate to touch living things?

  Ryota P. Kitani, Tatsuya Saga, Minoru Kasada, Mieko Kiyono, Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto

  The 29th Biennial Conference of Asian Association for Biology Education, Oct. 2024, English

  Poster presentation


• Environmental DNA approach in Eco Health/One Health towards eradication of opisthorchiasis

  Sato, M., Adisakwattana, P., Sayasone, S., Prasayasith, P., Valencia, J., Revolteado, M. J., Pongvongsa, T., Younuan, T., Minamoto, T., Sato, M. O.

  21st International Congress for Tropical Medicine and Malaria, Sep. 2024, English


• 迅速、効率的、かつ電力不要なeDNA核酸濃縮手法(QuickConc)の開発

  黒板智博, Qianqian Wu, 岩本遼, 源利文

  応用生態工学会 第２７回さいたま大会, Sep. 2024, Japanese

  Oral presentation


• 環境DNAを用いたセトウチサンショウウオ(Hynobius setouchi)の生息に影響する環境要因の推定

  松本奈々, 坂田雅之, 國政祐太, 山本優奈, 源利文

  応用生態工学会 第２７回さいたま大会, Sep. 2024, Japanese

  Poster presentation


• Estimating suitable breeding habitat for the Setouchi salamander (Hynobius setouchi) based on environmental DNA surveys

  Nana Matsumoto, Masayuki K. Sakata, Toshifumi Minamoto

  Salamander Meeting 2024, Jul. 2024, English

  Poster presentation


• クロダイの産卵検出における環境DNAの核/ミトコンドリア比の有効性

  石山颯人, 西田剛, 竹下大輝, 源利文, 笹野祥愛, 益田玲爾, 海野徹也, 河合賢太郎

  令和6年度日本水産学会春季大会, Mar. 2024, Japanese

  Poster presentation


• 環境DNAのメチル化解析に基づく魚類繁殖活動の検出

  平山一槻, 呉盧漢, 源利文

  第71回日本生態学会大会, Mar. 2024, Japanese

  Poster presentation


• 環境DNAを用いたニホンウナギ(Anguilla japonica)とオオウナギ(A. marmorata)の河川内分布特性

  恩田都和, 深野直孝, 三田村啓理, 久米学, 和田敏裕, 源利文, 渡邊俊, 高木淳一, 片山知史, 村上弘章

  第4回ウナギ学の現状, Mar. 2024, Japanese

  Oral presentation


• 環境DNA分析を用いたニホンウナギの分布規定要因の推定

  國政祐太, 橋本渚, 木谷亮太, 三田村啓理, 久米学, 田中智一朗, 源利文

  第4回ウナギ学の現状, Mar. 2024, Japanese

  Oral presentation


• Comparison of terrestrial mammal detection methods using environmental DNA

  Toshifumi Minamoto, Niko Nakamura, Nagisa Hashimoto, Masayuki K. Sakata, Qianqian Wu

  BES Annual Meeting 2023, Dec. 2023, English, British Ecological Society, Belfast, United Kingdom, International conference

  Poster presentation


• Methylation analysis of environmental DNA for breeding monitoring of fish

  Itsuki T. Hiyayama, Luhan Wu, Toshifumi Minamoto

  BES Annual Meeting 2023, Dec. 2023, English, British Ecological Society, Belfast, United Kingdom, International conference

  Poster presentation


• Development of detection methods for land mammals and birds using environmental DNA

  Niko Nakamura, Nagisa Hashimoto, Masayuki K. Sakata, Qianqian Wu, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  [Invited]

  Nominated symposium


• The Development and International Standardization of the Manual for Environmental DNA Analysis

  Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  [Invited]

  Nominated symposium


• Elucidation of the distribution of finless porpoise in Osaka Bay

  Nagisa Hashimoto, Ryota Kitani, Takashi Iwata, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Study on the breeding environment of Hynobius setouchi

  Saori Hisatomi, Masayuki K. Sakata, Niko Nakamura, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Distribution of native and non-native fish species in rivers

  Marina Otai, Nagisa Hashimoto, Yuta Kunimasa, Itsuki Hirayama, Yoshihiko Yamamoto, Masayuki K. Sakata, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Distribution survey of Opisthorchis viverrini and intermediate hosts in Savannakhet Province, Lao PDR

  Riko Matsuo, Natsumi Kihara, Megumi Sato, Marcello Otake Sato, Phoyphailin Prasayasith, Tippayarat Yoonuan, Poom Adisakwattana, Tiengkham Pongvongsa, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Implementation of environmental DNA education for high school students and verification of its effectiveness

  Ryota Kitani, Tatsuya Saga, Minoru Kasada, Mieko Kiyono, Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Estimation of factors regulating the distribution of Japanese eels in rivers

  Yuta Kunimasa, Nagisa Hashimoto, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Prediction of spatio-temporal distribution of organisms under climate change using environmental DNA analysis and ecological modeling

  Qianqian Wu, Jinxing Zhou, Tatsuya Komoto, Toshiyuki Ishikawa, Naoshige Goto, Masayuki K. Sakata, Daisuke Kitazawa, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Study on environmental nucleic acid extraction methods from highly turbid water samples

  Yuna Yamamoto, Ryo Iwamoto, Itsuki Hirayama, Ayumi Ogawa, Masayuki K. Sakata, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Monitoring Aquatic Plants in Irrigation Ponds Using Environmental DNA Metabarcoding Techniques

  Ayumi Ogawa, Saori Hisatomi, Nagisa Hashimoto, Masayuki K. Sakata, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• Development of detection assay for Schistosoma mekongi eDNA and its application in the field

  Nao Matsumura, Riko Matsuo, Masayuki K. Sakata, Qianqian Wu, Toshifumi Minamoto

  The 6th Annual Meeting of The eDNA Society, Dec. 2023, Japanese

  Poster presentation


• マリモの過去150年をミジンコで復元する

  占部城太郎, 大槻朝, 大竹裕里恵, 坂田雅之, 源利文, 加三千宣, 若菜勇

  日本生態学会東北地区会第68回大会, Nov. 2023, Japanese

  Oral presentation


• Prospects of eDNA analysis for macro–organisms: beyond species distribution

  Toshifumi Minamoto

  International Symposium: Past, Present, and Future of the Marine Environment and Ecosystems, Oct. 2023, English

  [Invited]

  Nominated symposium


• 堆積物DNAを用いた阿寒湖における魚類個体群動態の復元

  坂田雅之, 加三千宣, 大槻朝, 若菜勇, 源利文, 占部城太郎

  日本陸水学会第87回大分大会, Oct. 2023, Japanese

  Oral presentation


• 環境DNA分析を用いたニホンウナギのハビタット推定

  國政祐太, 橋本渚, 源利文

  日本陸水学会第87回大分大会, Oct. 2023, Japanese

  Poster presentation


• ホシガレイVerasper variegatusの環境DNA技術の確立と種苗放流後の分布推定

  村上弘章, 深野直孝, 鄔倩倩, 源利文, 髙木淳一, 久米学, 山下洋, 三田村啓理, 和田敏裕

  令和5年度日本水産学会秋季大会, Sep. 2023, Japanese

  Poster presentation


• 環境DNA分析を用いた大阪湾におけるスナメリの分布およびホットスポットの解明

  橋本渚, 岩田高志, 源利文

  日本哺乳類学会2023年度大会, Sep. 2023, Japanese

  Poster presentation


• The nuisance invasive species Cymbella janischii in Japan: knowledge from studies and monitoring

  Yoko Kato-Unoki, Tamie Suzawa, Akira Kurihara, Yuta Takahashi, Toshifumi Minamoto, Yoshitsugu Masuda, Jun-ichi Tsuboi, Satoquo Seino, Shigeki Mayama

  The 26th International Diatom Symposium, Aug. 2023, English

  Poster presentation


• Environmental DNA analysis of macroorganisms: recent trend and future prospects in Japan and Worldwide

  Toshifumi Minamoto

  The XXIIIrd International Congress of Genetics, Jul. 2023, English

  [Invited]

  Nominated symposium


• Development of eDNA research in Japan and its application to environmental studies

  Toshifumi Minamoto

  Biodiversity Genomics - A Global Perspective: Satellite Meeting of The XXIIIrd International Congress of Genetics, Jul. 2023, English

  [Invited]

  Nominated symposium


• Investigation of the effect of Artificial Light at Night (ALAN) on wild fish community ~manipulative field experiment and species composition analusis using eDNA methods~

  Aisha Oyabu, Luhan Wu, Natusmi Kihara, Hiroki Yamanaka, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Oral presentation


• Application of DNA methylation analysis to eDNA studies

  Itsuki Hirayama, Luhan Wu, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Oral presentation


• Investigation of the potential applicability of eDNA as an environmental education tool

  Ryota Kitani, Mieko Kiyono, Masayuki Sato, Atushi Ushimaru, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Horizontal and vertical distribution of environmental DNA of jack mackerel Trachurus japonicus and Japanese anchovy Engraulis japonicus in Maizuru Bay, Kyoto Prefecture

  Hiroaki Murakami, Keiichi Fukaya, Satoshi Yamamoto, Toshifumi Minamoto, Kenji Minami, Kazushi Miyashita, Taishin Kameoka, Reiji Masuda, Yoh Yamashita, Michio Kondoh

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Unraveling the effects of cross rover structures on the habitat of Japanese eel

  Yuta Kunimasa, Natsumi Kihara, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Distribution of finless porpoise (Neophicaena asiaeorientalis) in Osaka Bay, Japan using eDNA analysis

  Nagisa Hashimoto, Takashi Iwata, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Seasonal movement of Rainbow Trout detected by year-round water sampling

  Akio Imamura, Masayuki K. Sakata, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Fish population dynamics in Lake Akan associated with eutrophication reconstructed by sedementary DNA

  Masayuki K. Sakata, Michinobu Kuwae, Hajime Otsuki, Isamu Wakana, Toshifumi Minamoto, Jotaro Urabe

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Development of a highly sensitive eDNA detection method

  Ryo Iwamoto, Tomohiro Kuroita, Yuta Kunimasa, Yuna Yamanmoto, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Detection of Southeast Asian liver fluke by multi-region PCR assay

  Riko Matsuo, Megumi Sato, Mercello Sato, Poom Adisakwattana, Orawan Phuphisu, Tippayarat Yoonuan, Yanin Limpanon, Yupa Chusongsang, Paporn Poodeepiyasawa, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• Regional-scale effects of deer-induced forest degradation on river ecosystem dynamics

  Hikaru Nakagawa, Daisuke Fujiki, Hiroo Numata, Luhan Wu, Terutaka Mori, Toshifumi Minamoto

  The eDNA Society International Meeting 2023, May 2023, English

  Poster presentation


• 環境DNA分析で明らかにするさくら湖の魚類相

  源利文

  さくら湖自然環境フォーラム2022, Mar. 2023, Japanese

  [Invited]

  Public discourse


• 絶滅危惧種の保全と外来種対策における環境DNA分析の有効性

  源利文, 木原菜摘

  スイゲンゼニタナゴの保全を考えるワークショップ, Mar. 2023, Japanese

  [Invited]

  Nominated symposium


• 環境DNAを用いたスイゲンゼニタナゴと近縁外来種の同時検出系の開発と実践

  木原菜摘, 源利文

  第十八回外来魚情報交換会, Jan. 2023, Japanese

  Oral presentation


• マクロ生物の環境DNA分析: 生物分布から繁殖期推定まで

  源利文

  2023年 水生昆虫談話会・日本陸水学会共催シンポジウム, Jan. 2023, Japanese

  [Invited]

  Nominated symposium


• Environmental DNA in lake sediment provides fish information on reconstructing past fauna in lake ecosystems

  Masayuki K. Sakata, Narumi Tsugeki, Michinobu Kuwae, Hideyuki Doi, Toshifumi Minamoto

  BES Annual Meeting 2022, Dec. 2022, English

  Poster presentation


• 水中の生き物調査の新手法：環境DNA

  源利文

  公開シンポジウム「え、ニジマスって外来種なの？」, Dec. 2022, Japanese

  [Invited]

  Nominated symposium


• クモヒトデ綱(棘皮動物門) の環境DNAメタバーコーディング プライマーの開発

  岡西政典, 幸塚久典, 鄔倩倩, 進士淳平, 芝田直樹, 玉田貴, 中野智之, 源利文

  第18回棘皮動物研究集会, Dec. 2022, Japanese

  Oral presentation


• 棘皮動物における環境DNAメタバーコーディングプライマーの開発

  小泉佳祐, 中野智之, 鄔倩倩, 幸塚久典, 小川晟人, 岡西政典, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• クモヒトデ綱（棘皮動物門）の環境DNAメタバーコーディングプライマーの開発

  岡西政典, 幸塚久典, 鄔倩倩, 進士淳平, 芝田直樹, 玉田貴, 中野智之, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• 各組織が分散してデータを管理し、段階的にデータを共有・公開してデータの価値を高め活用する「データ価値共創環境」の提案

  坂本久, 庄司諒, 川北智弥, 坂田雅之, 山中裕樹, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• 環境水からの外来種ミズワタクチビルケイソウの特異的検出

  鵜木(加藤)陽子, 真山茂樹, 栗原暁, 清野聡子, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• スナメリの検出系開発と野外適用

  橋本渚, 木原菜摘, 坂田雅之, 中村清美, 源利文, 岩田高志

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• 河川横断構造物がニホンウナギの河川遡上に与える影響の解明

  國政祐太, 木原菜摘, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• 環境DNAにおけるDNAメチル化解析とその生態学的利用に向けたアプローチ

  平山一槻, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• 環境DNAを用いた陸上哺乳類の検出方法の開発

  中村仁湖, 橋本渚, 坂田雅之, 鄔倩倩, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• バラタナゴ属3種の新しい同時検出系の開発と実用可能性の検討～HRM法、マルチプレックスプローブ法の比較～

  木原菜摘, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• 定量メタバーコーディングを用いた魚類生態調査

  呉盧漢, 稲川崇史, 沖津二朗, 坂本正吾, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  [Invited]

  Poster presentation


• 環境DNAメタバーコーディングによる深海無脊椎動物の検出

  鄔倩倩, 西澤崚平, 藤原義弘, 土田真二, 吉田尊雄, 河戸勝, 駒井智幸, 源利文

  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese

  Poster presentation


• Detection of Opisthorchis viverrini using environmental DNA

  Riko Matsuo, Megumi Sato, Marcello Otake Sato, Poom Adisakwattana, Orawan Phuphisut, Tippayarat Yoonuan, Yanin Limpanont, Yupa Chusongsang, Paporn Poodeepiyasawat, Toshifumi Minamoto

  The 20th International Congress for Tropical Medicine and Malaria, Oct. 2022, English

  Poster presentation


• ダム湖の生物モニタリングに環境DNA技術を適用するためには？

  源利文

  ELR2022, Sep. 2022, Japanese

  [Invited]

  Nominated symposium


• 堆積物環境DNAによる琵琶湖魚類の過去復元

  坂田雅之, 槻木玲美, 加三千宣, 土居秀幸, 源利文

  日本陸水学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• 夜間の光は魚の生態に影響を及ぼすのか？ー環境DNA技術を用いた種組成調査よりー

  大藪愛紗, 源利文

  日本陸水学会第86回大会, Sep. 2022, Japanese

  Poster presentation


• 野生動物の糞便からの薬剤耐性大腸菌の分離と性状解析

  浅井鉄夫, 杉山美千代, 大松勉, 源利文

  令和4年度獣医学術中部地区学会, Aug. 2022, Japanese

  Oral presentation


• 環境DNAと種分布モデルを用いた島根県におけるオオサンショウウオの生息実態の解明

  リュウゲンテイ, 橋本真佑, 山岸聖, 三浦祐児, 源利文, 高原輝彦

  2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese

  Oral presentation


• 環境RNA手法の実用化に向けた検証：ニホンウナギをモデルケースにした野外調査と飼育実験から

  永田晃弘, 辻井彩花, 山岸聖, 源利文, 山中裕樹, 高原輝彦

  2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese

  Oral presentation


• 堆積物コアDNAを用いた宍道湖におけるマクロ生物の近過去生息状況の推定

  山岸聖, 小室隆, 神門利之, 引野愛子, 坂田雅之, 源利文, 下田莉奈, 高原輝彦

  2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese

  Oral presentation


• 外来種ミズワタクチビルケイソウ検出法の開発

  鵜木(加藤)陽子, 真山茂樹, 栗原暁, 清野聡子, 源利文

  令和4年度日本水産学会春季大会, Mar. 2022, Japanese

  Poster presentation


• Reconstruction of past Charales algae in Lake Shinji using seda DNA (in Japanese)

  小室隆, 神門利之, 加藤季晋, 引野愛子, 山岸聖, 高原輝彦, 後藤益滋, 坂田雅之, 源利文

  2022年日本地理学会春季学術大会, Mar. 2022, Japanese

  Oral presentation


• Search for reservoir animals of antimicrobial resistant bacteria using environmental DNA analysis

  Nagisa Hashimoto, Masayuki K. Sakata, Michiyo Sugiyama, Tetsuo Asai, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• The potential of abandoned river as habitats for fish species in Lake Biwa

  Hayato Higashisaka, Takehiro Matsumoto, Masayuki K. Sakata, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• Optimization of survey design for environmental DNA analysis using a multispecies site occupancy model

  Takehiro Matsumoto, Keiichi Fukaya, Masayuki K. Sakata, Jiro Okitsu, Takashi Inagawa, Kosuke Hiraoka, Hidetaka Ichiyanagi, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• Field application of a new eDNA metabarcoding assay using nuclear DNA as a marker

  Daisuke Sasaki, Gen Ito, Hiroki Yamanaka, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• Simultaneous monitoring of the spawning period of multiple fishes using quantitative environmental DNA metabarcoding

  Luhan Wu, Takashi Inagawa, Jiro Okitsu, Syogo Sakamoto, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• Investigation of an effective method to detect environmental DNA targeting lotic salamander species

  Masayuki K. Sakata, Daiki Takeshita, Ryohei Nishizawa, Takuya Sato, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• Impact of the change from a full-circulation to a partial-circulation lake on benthic organisms

  Qianqian Wu, Jinxin Zhouu, Tatsuya Komoto, Toshiyuki Ishikawa, Masayuki K. Sakata, Naoshige Goto, Daisuke Kitazawa, Toshifumi Minamoto

  The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, Japanese

  Poster presentation


• Review of integrated research on human well-being, ecosystem services, and spatial characteristics of cities: research trends and future directions

  Yuta Uchiyama, Masayuki Sato, Atishi Ushimaru, Toshifumi Minamoto

  3rd ESP Asia Conference, Dec. 2021, English

  Oral presentation


• 環境DNA分析の基礎と魚病を含む感染症への応用

  源利文

  令和３年度 魚病症例研究会, Nov. 2021, Japanese

  [Invited]

  Keynote oral presentation


• 大阪府内における環境DNA分析技術を用いた淡水魚分布調査の検討

  山本義彦, 鴛海智佳, 白子智康, 源利文

  第24回自然系調査研究機関連絡会議, Japanese

  Poster presentation


• Novel universal primers for metabarcoding environmental DNA surveys of cephalopods

  Qianqian Wu, Tomoyuki Nakano, Tomoyuki Komai, Masaki Miya, Luhan Wu, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Invited oral presentation


• 海洋堆積物のDNA量から魚類個体数の数十年スケール変動を捉えられるか

  加三千宣, 玉井弘道, 土居秀幸, 源利文, 坂田雅之

  2021年度水産海洋学会研究発表大会, Nov. 2021, Japanese


• Improved accuracy of environmental DNA metabarcoding analysis using thresholds obtained from the defined copy number of DNA

  Yusuke Osaki, Kazuki Watanabe, Yuki Yonekawa, Satoshi Nakazawa, Hirotaka Unno, Eri Nishiyama, Hiroaki Murakami, Takahiro Matsudaira, Masayuki K. Sakata, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Investigation of long-term storage method of environmental DNA glass filter samples at low and room temperature

  Hiroki Hashizume, Toshifumi Minamoto, Shin-ya Ohba, Kazuhiko Moji, Tomonori Hoshi

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Comparison of fish detection results by environmental DNA according to tidal conditions, water sampling volume, and water sampling depth in the coastal waters of Suooshima, Yamaguchi Prefecture

  Etsujiro Katayama, Tomoyuki Kohama, Toshikazu Tatematsu, Takeshi Watanabe, Koichi Kumei, Koji Kaneko, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Is it possible to replace capture surveys? -Impact of primers and DNA databases on macroinvertebrate detection-

  Yuta Hasebe, Toshifumi Minamoto, Masaki Takenaka, Yoshihiro Handa, Masafumi Seki, Tomoyasu Shirako

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Fish eDNA analysis of seawater and sediment at offshore Nobeoka City Miyazaki Prefecture

  Toshikazu Tatematsu, Etsujiro Katayama, Tomoyuki Kohama, Nobutaka Maki, Takeshi Kanbashira, Ichiro Toridamari, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Attempt of fish eDNA metabarcoding in a lake with algal blooms

  Qianqian Wu, Masayuki K. Sakata, Deyi Wu, Hiroki Yamanaka, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Development and evaluation of PCR primers for environmental DNA metabarcoding of Amphibia

  Masayuki K, Sakata, Mone, U. Kawata, Atsushi Kurabayashi, Takaki Kurita, Masatoshi Nakamura, Tomoyasu Shirako, Ryosuke Kakehashi, Kanto Nishikawa, Mohamad Yazid Hossman, Takashi Nishijima, Junichi Kabamoto, Masaki Miya, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Development of eDNA metabarcoding assay targeting nuclear DNA as a marker for fish

  Daisuke Sasaki, Hiroki Yamanaka, Masayuki K. Sakata, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Spatio-temporal changes of environmental DNA concentration due to fish spawning activity

  Luhan Wu, Yoshihiko Yamamoto, Shogo Yamaguchi, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Towards the implementation of environmental DNA metabarcoding for fish surveys in dam reservoirs

  Takehiro Matsumoto, Keiichi Fukaya, Masayuki K. Sakata, Jiro Okitsu, Takashi Inagawa, Kosuke Hiraoka, Hidetala Ichiyanagi, Toshifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Development of eDNA metabarcoding assays for Anthozoa

  Ryohei Nishizawa, Qianqian Wu, Tomoyuki Nakano, Tomoyuki Komai, Tohifumi Minamoto

  The 4th Meeting of The eDNA Society, Nov. 2021, Japanese

  Poster presentation


• Detection of a group of pathogenic fungi in soils of mycetoma endemic region in the arid area of the Republic of Sudan

  Hiroki Hashizume, Suguru Taga, Masayuki K, Sakata,Mahmou, Hussein Mohamed Taha, Emmanuel Edwar Siddig,Toshifumi Minamoto,Ahmed, Hassan Fahal, Satoshi Kaneko

  The 34th JSME Meeting, Oct. 2021, English

  Poster presentation


• 環境DNA技術を感染症の分布診断に応用する

  源利文

  日本生態学会第24回公開講演会 （環境DNAの衝撃：生き物たちの過去・現在・未来を解き明かす）, Mar. 2021, Japanese

  [Invited]

  Public discourse


• 産卵と被食に伴う核およびミトコンドリア環境DNAの動態:カタクチイワシとマアジを用いた水槽実験

  上村真太郎, 益田玲爾, 村上弘章, 徐寿明, 源利文

  令和３年度日本水産学会春季大会, Mar. 2021, Japanese

  Poster presentation


• 感染症の生態学研究のためのツールとしての環境DNA

  源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 魚類環境RNAの検出最適化のためのプロトコル検証および野外適用

  高尾健太, 徐寿明, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境核酸分析によるコイの栄養状態の評価

  姜明揚, 山本義彦, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 核ＤＮＡをマーカーとした魚類環境ＤＮＡメタバーコーディングの新規手法の開発

  佐々木大介, 山中裕樹, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境DNAを用いたメダカにおける遺伝的撹乱の検出

  中尾遼平, 北川忠生, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• ニホンジカ(Cervus nippon)環境DNAの種特異的検出系の開発

  石坂恵規, 中川光, 高柳敦, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境DNA分析によるオオダイガハラサンショウウオの分布予測

  竹下大輝, 西澤崚平, 佐藤拓哉, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境DNAを用いたバラタナゴ属3種の同時検出系確立と野外適用

  木原菜摘, 坂田雅之, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境DNAを用いたダム湖における魚類繁殖期の推定

  呉盧漢, 稲川崇史, 沖津二郎, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• アオコの発生した湖におけるeDNA メタバーコーディング: 中国太湖を事例として

  邬倩倩, 坂田雅之, 吴德意, 山中裕樹, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境DNAとRNAの分解に及ぼす温度とpHの影響

  釣健司, 廣原嵩也, 島田康人, 源利文, 山中裕樹

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 琵琶湖の湖底貧酸素化に伴うスジエビ及びイサザへの影響について

  河本達也, 鄔倩倩, 坂田雅之, 石川俊之, 後藤直成, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 鉢虫綱クラゲ類の環境DNAメタバーコーディング系の設計

  西澤崚平, 邬倩倩, 中野智之, 駒井智幸, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 環境DNAメタバーコーディングを用いたトンボ目多様性の評価

  矢指本哲, 坂田雅之, 中尾遼平, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 堆積物環境DNAを用いた琵琶湖における過去生物情報の再構築

  坂田雅之, 槻木玲美, 加三千宣, 土居秀幸, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• ダム湖において環境DNAメタバーコーディング手法を用いる際の最適採水地点数の検討

  松本岳大, 深谷肇一, 坂田雅之, 稲川崇史, 沖津二朗, 平岡康介, 一柳英隆, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• 長鎖・核DNAおよび海水サンプルを対象とした塩化ベンザルコニウムの環境DNA保存効果

  徐寿明, 村上弘章, 益田玲爾, 源利文

  日本生態学会第68回大会, Mar. 2021, Japanese

  Poster presentation


• Using environmental nucleic acids in water to understand animal behavior and physiology

  Toshifumi Minamoto

  Kyoto University Center for Ecological Research Joint Research Meeting: New Challenges in Environmental DNA and RNA Research, Dec. 2020, Japanese

  [Invited]

  Nominated symposium


• Development and implementation of a survey method for underwater organisms using environmental DNA

  Toshifumi Minamoto

  FY2020 Fisheries Resource Conservation Awareness Research Project: Workshop on Environmental DNA Research and Development, Dec. 2020, Japanese

  [Invited]

  Public discourse


• Environmental DNA preservation in sediment with application to detecting jellyfish blooms after the tsunami

  Mizuki Ogata, Reiji Masuda, Hiroya Harino, Masayuki K. Sakata, Makoto Hatakeyama, Katsuhide Yokoyama, Yoh Yamashita, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• Detection of environmental DNA of snakes in paddy fields

  Ryohei Nishizawa, Ryohei Nakao, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• Detection of spawning behavior of Cyprinidae fish by eDNA analysis

  Daisuke Sasaki, Takeshi Watanabe, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• Estimation of the spawning period of Japanese sea cucumber, Apostichopus japonicus

  Daiki Takeshita, Hiroaki Murakami, Reiji Masuda, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• Investigating an effective sampling method for fish surveys using environmental DNA metabarcoding

  Masayuki K. Sakata, Takeshi Watanabe, Nobutaka Maki, Kousuke Ikeda, Toshihiro Kosuge, Hiroaki Okada, Hiroki Yamanaka, Tetsuya Sado, Masaki Miya, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• State-dependent persistence of environmental DNA

  Toshiaki Jo, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• Estimating the spawning period of fish species using environmental DNA

  Luhan Wu, Takashi Inagawa, Jiro Okitsu, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• The influence of marine fish mixed with other species on environmental DNA emission

  Hiroaki Murakami, Reiji Masuda, Satoshi Yamamoto, Toshifumi Minamoto, Yoh Yamashita

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• The effect of body size and activity on eDNA in red-spotted grouper Epinephelus akaara

  Mikihiro Sonetaka, Reiji Masuda, Toshiaki, Jo, Daiki Takeshita, Hiroaki Murakami, Shintaro Kamimura, Toshifumi Minamoto

  The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, Japanese

  Poster presentation


• Study on intermediate and reservoir hosts of Opisthorchis viverrini in high endemic area in Prey Veng Province, Cambodia

  Masashi Kirinoki, Marcello Otake Sato, Khieu Virak, Toshifumi Minamoto, Yuichi Chigusa, Kazuko Miyamoto

  Joint Congress on Global Health 2020 in Osaka, Nov. 2020, Japanese

  Poster presentation


• Does the release of environmental DNA vary with growth stage ? a case study using Japanese giant salamander

  MORIMOTO Teppei, TAGUCHI Yuki, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Species composition of native freshwater fish and alien Rainbow Trouts in Hokkaido Island using eDNA barcoding

  IMAMURA Akio, HAYAMI Kana, SAKATA Masayuki K., MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Javanese

  Poster presentation


• Distribution of smallmouth bass and channel catfish in Yodo River: evaluation using environmental DNA

  YAMAMOTO Yoshihiko, HONGO Masamichi, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Improving the accuracy in eDNA analysis using threshold based on known number of DNA

  WATANABE Kazuki, MINAMOTO Toshifumi, MATSUDAIRA Takahiro, OSAKI Yusuke, YONEKAWA Yuki, SUZUKI Takeru, HASHIMOTO Michie, UNNO Hirotaka

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Study on disease ecology via environmental DNA analysis

  MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Estimating spawning behavior of common carp with environmental RNA analysis

  JIANG Mingyang, YAMAMOTO Yoshihiko, WU Luhan, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• What physiological factors does the shedding of environmental DNA associate?

  JO Toshiaki, MURAKAMI Hiroaki, MASUDA Reiji, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• The ecology and genetic structure of polymorphic migratory behavior of Palaemon paucidens in Lake Biwa

  WU Qianqian, YAIDA Yuki, SAWADA Hayato, MARUYAMA Atsushi, TAKAMI Yasuoki, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Distribution of Schistosoma environmental DNA in Lake Victoria

  OSAWA Ryousuke, NAKAMURA Risa, FUTAMI Kyoko, ITAYAMA Tomoaki, KIKUCHI Mihoko, OUMA Colins, HAMANO Shinjiro, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Evaluation of regenerated reed zone as spawning habitat using environmental DNA analysis

  SASAKI Daisuke, WATANABE Takeshi, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Seasonal changes in the structure of the fish community including the endangered loach Parabotia curtus, inferred from environmental DNA

  SUGIURA Ko, IWATA Akihisa, ABE Tsukasa, MINAMOTO Toshifumi, TOMITA Sei, YAMAMOTO Satoshi, MISHINA Tappei, WATANABE Katsutoshi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Restoration of past biological information by sedimentary environmental DNA analysis in Lake Biwa

  SAKATA Masayuki K., TSUGEKI Narumi, KUWAE Michinobu, OCHI Natsuki, HAYAMI Kana, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Estimation of reservoir animals of antibiotic resistant bacteria by environmental DNA metabarcoding for mammals

  HAYAMI Kana, SAKATA Masayuki K., ARATANI Tomoki, ASAI Tetsuo

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Can snakes be detected by eDNA analysis?

  NISHIZAWA Ryohei, NAKAO Ryohei, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Observation of eDNA in a breeding season of Siberian salamander

  TAKESHITA Daiki, TERUI Shigeharu, IKEDA Kousuke, MITSUZUKA Takashi, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Estimating the breeding period of wild fish using environmental DNA

  Wu Luhan, JIANG Migyang, YAMAMOTO Yoshihiko, INAGAWA Takashi, OKITSU Jiro, MINAMOTO Toshifumi

  The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, Japanese

  Poster presentation


• Monitoring biodiversity using environmental DNA

  MINAMOTO Toshifumi

  Lecture "Biodiversity of coastal seas and restoring environment", Feb. 2020, Japanese

  [Invited]

  Public discourse


• Environmental DNA surveys and its application

  MINAMOTO Toshifumi

  JEAS Seminar FY2019, Feb. 2020, Japanese

  [Invited]

  Public discourse


• An attempt to understand the distribution of native fish communities and rainbow trout in Hokkaido by eDNA metabarcoding

  IMAMURA Akio, HAYAMI Kana, SAKATA Masayuki K., MINAMOTO Toshifumi

  Meeting of Hokkaido Natural History Rsearch Society 2019, Feb. 2020, Japanese

  Oral presentation


• Biological survey using environmental DNA: from the basis to newest researches

  MINAMOTO Toshifumi

  MEC Seminar, Dec. 2019, Japanese

  [Invited]

  Public discourse


• Monitoring breeding behavior of Japanese giant salamander via eDNA analysis

  MINAMOTO Toshifumi, MORIMOTO Teppei, TAGUCHI Yuki, RAKAO Ryohei

  British Ecological Society Annual Meeting 2019, Dec. 2019, English, International conference

  Oral presentation


• Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA

  SAKATA Masayuki K, YAMAMOTO Satoshi, GOTOH Ryo, MIYA Masaki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  British Ecological Society Annual Meeting 2019, Dec. 2019, English, International conference

  Poster presentation


• Particle size distribution of environmental DNA from fish nuclei and mitochondria

  JO Toshiaki, ARIMOTO Mio, MURAKAMI Hiroaki, MASUDA Reiji, MINAMOTO Toshifumi

  The 42nd Annual Meeting of the Molecular Biology Society of Japan, Dec. 2019, Japanese, Domestic conference

  Poster presentation


• Environmental DNA (eDNA) analysis of pathogenic Leptospira and associated organisms in leptospirosis-endemic Okinawa areas

  Sato, Y, Mizuyama, M, Sato, M, Minamoto, T, Kimura, R, Toma, C

  The 4th Kuroshio Conference, Nov. 2019, English, Domestic conference


• Improvement of environmental DNA detection of Schistosoma mansoni: comparison of filtration methods

  OSAWA Ryosuke, NAKAMURA Risa, FUTAMI Kyoko, ITAYAMA Tomoaki, KIKUCHI Mihoko, HAMANO Shinjiro, MINAMOTO Toshifumi

  The 60th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2019, Japanese, Domestic conference

  Poster presentation


• Environmental DNA as a novel tool for clarifying disease ecology

  MINAMOTO Toshifumi

  The 60th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2019, English, Domestic conference

  [Invited]

  Nominated symposium


• Opisthorchiasis and schistosomiasis from the environmental DNA/GIS perspective

  SATO Marcello Otake, RAFALIMANANTSOA Armand, SATO Megumi, PONGVONGSA Tiengkham, MINAMOTO Toshifumi, WAIKAGUL Hitra, KAWAI Satoru, FONTANILLA Ian, CHIGUSA Yuichi

  The 60th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2019, English, Domestic conference

  [Invited]

  Nominated symposium


• Dynamics of environmental DNA derived from nuclei and mitochondria

  JO Toshiaki, ARIMOTO Mio, MURAKAMI Hiroaki, MASUDA Reiji, MINAMOTO Toshifumi

  The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conference

  Nominated symposium


• Improvement of environmental DNA detection method for Schistosoma mansoni

  OSAWA Ryosuke, NAKAMURA Risa, FUTAMI Kyoko, ITAYAMA Tomoaki, KIKUCHI Mihoko, OUMA Collins, HAMANO Shinjiro, MINAMOTO Tosnifumi

  The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conference

  Poster presentation


• Observation of small amphibians in wetland using environmental DNA

  TAKESHITA Daiki, TERUI Shigeharu, IKEDA Kosuke, MITSUZUKA Takashi, OSATHANUNKUL Maslin, MINAMOTO Toshifumi

  The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conference

  Poster presentation


• Vertical distribution of fish environmental DNA in a dam reservoir

  HAYAMI Kana, SAKATA Masayuki K, OKITSU Jiro, INAGAWA Takashi, MINAMOTO Toshifumi

  The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conference

  Poster presentation


• Elucidation of the life history of Palaemon paucidens in Lake Biwa

  WU Qianqian, MINAMOTO Toshifumi

  The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conference

  Poster presentation


• Elucidation of seasonal movement of Acheilognathus longipinnis using environmental DNA and relationship with environmental characteristics

  NAGAYAMA Shigeya, OHTA Munehiro, KATO Masayuki, MINAMOTO Toshifumi, MORI Seiichi

  The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conference

  Poster presentation


• Changes of the concentration of environmental DNA of fish before and after flooding-a case study in a lentic water-

  INAGAWA Takashi, OKITSU Jiro, MINAMOTO Toshifumi, MURAOKA Keiko, NAKAMURA Keigo

  The 2nd Annual Meeting of The eDNA Society, Sep. 2019, Japanese, Domestic conference

  Poster presentation


• Toward the implementation of fish monitoring using environmental DNA

  MINAMOTO Toshifumi

  The 23rd Annual Meeting of the Ecology and Civil Engineering Society, Sep. 2019, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• Application of bamboo biomass resources in agrochemical-free rice farming: effects on Odonata diversity

  HUYNH Thien, Quang, SAKATA Masayuki, K, NAKAO Ryohei, NOMURA Shinya, KLAILATI Masfiro, MINAMOTO Toshifumi, USIO Nishikawa

  The 84th Annual Meeting of the Japanese Society of Limnology, Sep. 2019, English, Domestic conference

  Oral presentation


• Search for a rare freshwater fish, Acheilognathus typus, and related organisms through environmental DNA metabarcoding

  SAKATA Masayuki K, DOI Hideyuki, MAKI Nobutaka, UEDA Natsuki, SUGIYAMA Hideki, MINAMOTO, Toshifumi

  The 84th Annual Meeting of the Japanese Society of Limnology, Sep. 2019, Japanese, Domestic conference

  Oral presentation


• Water temperature and biomass dependence of nucleus and mitochondria environmental DNA of Japanese jack mackerel

  JO Toshiaki, ARIMOTO Mio, MURAKAMI Hiroaki, MASUDA Reiji, MINAMOTO Toshifumi

  2019 Autumn Meeting of Japanese Society of Fisheries Science, Sep. 2019, Japanese, Domestic conference

  Oral presentation


• On the efficacy of eDNA metabarcoding for a basin scale fish survey

  KATO Atsuko, OKADA Yasuaki, WATANABE Takeshi, MAKI Nobutaka, KOSUGE Toshihiro, IKEDA Kosuke, MATSUSHIMA Yukako, SAKATA Masayuki K, MINAMOTO Toshifumi

  Japan Society of Civil Engineers 2019 Annual Meeting, Sep. 2019, Japanese, Kagawa University, Takamatsu, Japan, Domestic conference

  Oral presentation


• On the detection accuracy of fish species in Koidegawa river by the difference in sampling volume and sampling position

  MAKI Nobutaka, WATANABE Takeshi, OKADA Yasuaki, KOSUGE Toshihiro, IKEDA Kosuke, MATSUSHIMA Yukako, SAKATA Masayuki K, MINAMOTO TOSHIFUMI

  Japan Society of Civil Engineers 2019 Annual Meeting, Sep. 2019, Japanese, Kagawa University, Takamatsu, Japan, Domestic conference

  Oral presentation


• The applicability of sedimentary environmental DNA analysis for restoration of past biological information

  SAKATA Masayuki, MINAMOTO Toshifumi

  21st Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2019, Japanese, Hokkaido University, Sapporo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Estimating population abundance using environmental DNA

  FUKAYA Keiichi, MURAKAMI Hiroaki, YOON Seokjin, MINAMI Kenji, OSADA Yutaka, YAMAMOTO Satoshi, MASUDA Reiji, KASAI Akihide, MIYASHITA Kazushi, MINAMOTO Toshifumi, KONDOH Michio

  21st Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2019, Japanese, Hokkaido University, Sapporo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Environmental DNA for the ecology of infectious diseases

  MINAMOTO Toshifumi

  21st Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2019, Japanese, Hokkaido University, Sapporo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Distributional pattern of black sea bream Acanthopagrus schlegelii estimated from environmental DNA: seasonal changes in marine and river waters

  SASANO Sachia, MURAKAMI Hiroaki, SUZUKI Keita W, YAMASHITA Yoh, MASUDA Reiji, MINAMOTO Toshifumi

  Fisheries Society of the British Isles, Symposium 2019, Jul. 2019, English, University of Hull (Hull City, United Kingdom), International conference

  Oral presentation


• Biomass-dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles

  HORIUCHI Tomoya, MASUDA Reiji, MURAKAMI Hiroaki, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  Fisheries Society of the British Isles, Symposium 2019, Jul. 2019, English, University of Hull (Hull City, United Kingdom), International conference

  Poster presentation


• Detection of eDNA of alien ant speciesfrom soil samples

  YASASHIMOTO Tetsu, SAKATA Masayuki K, SAKIDA Tomoya, OZAKI Mamiko, NAKAJIMA Tomoko, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Detection of common carp using eRNA from water samples

  JIANG Mingyang, MAEGAWA Kazuya, NAKAO Ryohei, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Application of environmental DNA method in tropical wetland: example of Schistosoma mansoni in Lake Victoria, Kenya

  OSAWA Ryosuke, ITAYAMA Tomoaki, FUTAMI Kyoko, HAMANO Shinjiro, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Improvement of eDNA extraction method from sedimental samples

  SAKATA Masayuki K, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Establishment of eDNA metabarcoding method for aquatic beetles

  INOUE Aoi, NAKAO Ryohei, MINAMOTO Toshifumi, SHINOHARA Tadashi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Estimation of mammal communities using environmental DNA from water and soil samples

  HAYAMI Kana, SAKATA Masayuki K, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Detection and quantification of macroorganisms using species-specific environmental DNA analysis

  MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Environmental DNA as a tool for citizen science

  MINAMOTO Toshifumi

  FY2018 Institute of Human Development Symposium, Mar. 2019, Japanese, Kobe University, Kobe, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Estimation of the source of environmental DNA by gene-specific environmental RNA analysis focusing on tissue-specific messenger RNA expressions

  TSURI Kenji, IKEDA Shizuya, HIROHARA Takaya, SHIMADA Yasuhito, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Using environmental DNA to detect the spatial and temporal distribution of Palaemonpaucidens in Lake Biwa

  WU Qianqian, KAWANO Ken, ISHIKAWA Toshiyuki, SAKATA Masayuki K, NAKAO Ryohei, HIRAIWA Masayoshi K, TSUJI Satsuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Habitat identification of Hynobius kimurae in Rokko mountains using environmental DNA analysis

  KISHIMOTO Akira, SUZUKI Takanori, NAKAMURA Toshiki, SAITO Hiroyuki, IIDUKA Tetsuya, YOKOTA Masahiro, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Oral presentation


• An attempt to quantify of invasive fishusing environmental DNA analysis

  GOTO Narumi, HAYAMI Kana, OKITSU Jiro, INAGAWA Takashi, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• An attempt for the distribution survey of Japanese giant salamander in Shiga Prefecture, Japan

  NAKAGAWA Akira, WAKAYAMA Shunto, SIROMOTO Yuki, TANAKA Satoma, MITO Naoki, MINAMOTO Toshifumi, NISHIKAWA Kanto, MATSUI Masafumi, SAITO Osamu

  2019 Meeting of Kansai Organization for Nature Conservation, Mar. 2019, Japanese, Tasaka Museum of Natural History, Osaka, Japan, Domestic conference

  Poster presentation


• Environmental DNA reveals the relationships among land uses and freshwater fish fauna around Mts. Rokko

  NAKAO Ryohei, HIRAIWA Masayoshi K, YAMAMOTO Satoshi, MIYA Masaki, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Capturing spawning migration of the endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus), using environmental DNA

  YATSUYANAGI Tetsu, KANBE Takashi, MIZUMOTO Hiroki, KOBAYASHI Yumi, SAKATA Masayuki K, MINAMOTO Toshifumi, ISHIDA Ryotaro, NII Hisaya, ARAKI Hitoshi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Estimation of eDNA shedding and degradation rates of Japanese jack mackerel using nuclear DNA marker

  ARIMOTO Mio, JO Toshiaki, MURAKAMI Hiroaki, MASUDA Reiji, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Evaluating the effectiveness of control measures for invasive red-eared slider turtles using environmental DNA

  KANAYAMA Akiri, IIDUKA Tetsuya, YOKOTA Masahiro, SUZUKI Takanori, KISHIMOTO Akira, NAKAMURA Toshiki, SAITO Hiroyuki, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Amphibians environmental DNA detection using metabarcoding analysis

  KAWATA Mone, KURABAYASHI Atsushi, RAMAMONJISOA Noerikanto, NATSUHARA Yoshihiro, YAMANAKA Hiroki, SATO Hirotoshi, SAKATA Masayuki K, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Establishment of environmental DNA detection system of Salamandrella keyserlingii and its application in Kushiro Marsh

  TAKESHITA Daiki, TERUI Shigeharu, IKEDA Kousuke, MITSUZUKA Takashi, OSATHANUNKUL Maslin, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Can breeding behavior of Japanese giant salamander detect just by water?~Based on the results of the environmental DNA survey in artificial nests~

  MORIMOTO Teppei, TAGUCHI Yuki, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Visualizing environmental DNA using cytogenetics

  JO Toshiaki, MURAKAMI Hiroaki, MASUDA Reiji, OHMIDO Nobuko, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, English, Kobe Convention Center, Kobe, Japan, Domestic conference

  Oral presentation


• The use of SNP markers to detect genetic variation in environmental DNA

  UCHII Kimiko, DOI Hideyuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Application of bamboo biomass resources in agrochemical-free rice farming: effects onodonate diversity

  HUYNH Thien Quang, SAKATA Masayuki K, NAKAO Ryohei, MINAMOTO Toshifumi, LAILATI Masfiro, USIO Nisikawa

  The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, English, Kobe Convention Center, Kobe, Japan, Domestic conference

  Poster presentation


• Monitoring of aquatic organisms using environmental DNA

  MINAMOTO Toshifumi

  2019 Academic Meeting of Tottori Prefectural Institute of Public Health and Environmental Science, Feb. 2019, Japanese, Tottori University, Tottori, Japan, Domestic conference

  Oral presentation


• Distribution survey for rare or invasive species by species-specific detection of eDNA

  MINAMOTO Toshifumi

  64th Seminar of Japan Society on Water Environment, Jan. 2019, Japanese, Jidousya-Kaikan, Tokyo, Japan, Domestic conference

  Public discourse


• On the development of eDNA analysis and basic techniques

  MINAMOTO Toshifumi

  Symposium of Marine Biodiversity Research Area of CREST program, Jan. 2019, Japanese, Sasagawa Peace Foundation Building, Tokyo, Japan, Domestic conference

  Public discourse


• Looking into the world of aquatic organisms: environmental DNA

  MINAMOTO Toshifumi

  Lecture for High School Teachers in Osaka, Dec. 2018, Japanese, Viale Osaka, Osaka, Japan, Domestic conference

  Public discourse


• Applicability of eDNA analysis: estimation of biomass and multispecies-detection

  MINAMOTO Toshifumi

  Kickoff Symposium of eDNA Research Center, Yamaguchi University, Dec. 2018, Japanese, International Hotel Ube, Ube, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Spatio-temporal detection patterns of fish eDNA in lentic ecosystems

  MINAMOTO Toshifumi, HAYAMI Kana, SAKATA Masayuki K, OKITSU Jiro, MIYA Masaki, GOTOH Ryo O, SATO Hirotoshi, YAMANAKA Hiroki

  British Ecological Society Annual Meeting 2018, Dec. 2018, English, Birmingham ICC, Birmingham, UK, International conference

  Poster presentation


• High water temperature and fish biomass can accelerate the shedding and degradation of the Japanese jack mackerel (Trachurus japonicus) environmental DNA

  JO Toshiaki, MURAKAMI Hiroaki, YAMAMOTO Satoshi, MASUDA Reiji, MINAMOTO Toshifumi

  British Ecological Society Annual Meeting 2018, Dec. 2018, English, Birmingham ICC, Birmingham, UK, International conference

  Poster presentation


• Environmental DNA: a different approach for food/water borne helminths studies

  SATO Marcello Otake, RAFALIMANANTSOA Armand, SATO Megumi, PONGVONGSA Tiengkham, MINAMOTO Toshifumi, WAIKAGUL Jitra, KAWAI Satoru, FORNILLOS Raffy Jay C, TABIOS Ian Kim B, LEONARD Lydia R, FONTANILLA Ian Kendrich C, CHIGUSA Yuichi

  Joint International Triopical Medicine Meeting 2018, Dec. 2018, English, Amari Watergate Bangkok, Bangkok, Thailand, International conference

  Oral presentation


• Detection of zebrafish messenger RNAs from tank water: implications for the practicability of environmental RNA analysis for aquatic macroorganisms

  YAMANAKA Hiroki, TSURI Kenji, IKEDA Seiya, SHIMADA Yasuhito, MINAMOTO Toshifumi

  British Ecological Society Annual Meeting 2018, Dec. 2018, English, Birmingham ICC, Birmingham, UK, International conference

  Poster presentation


• Aquatic survey by water collection: introduction of eDNA techniques

  MINAMOTO Toshifumi

  Kinki Meeting of the Institution of Professional Engineers, Japan, Nov. 2018, Japanese, Urbanex Bingo-cho, Osaka, Japan, Domestic conference

  Public discourse


• Environmental DNA: from micro- to macro-organisms

  MINAMOTO Toshifumi

  The 41st Annual Meeting of Molecular Bilogy Society of Japan, Nov. 2018, Japanese, Pacifico Yokohama, Yokohama, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Environmental DNA: a new method of monitoring underwater organisms

  MINAMOTO Toshifumi

  Kobe City Symposium on Biodiversity, Nov. 2018, Japanese, Ozi Zoo, Kobe, Japan, Domestic conference

  Public discourse


• Fish monitoring in dam lakes by using environmental DNA

  HAYAMI Kana, SAKATA Masayuki K, OKITSU Jiro, KATANO Izumi, MIYA Masaki, GOTOH Ryo O, SATO Hirotoshi, YAMANAKA Hiroki, MINAMOTO Toshifumi

  Ecology and Civil Engineering Society Sendai Branch Symposium, Nov. 2018, Japanese, Mahara, Miharu, Fukushima, Japan, Domestic conference

  Poster presentation


• Application of eDNA analysis to fish distribution survey

  MINAMOTO TOSHIFUMI

  Japanese Society of Fisheries Oceanography Symposium, Nov. 2018, Japanese, The University of Tokyo, Kashiwa, Chiba, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Improvement of sedimentary environmental DNA extraction method and prospect for restoration of past fauna

  SAKATA Masayuki K, MINAMOTO Toshifumi

  The 83rd Meeting of theJapanese Society of Limnology, Oct. 2018, Japanese, Okayama University, Okayama, Japan, Domestic conference

  Oral presentation


• Underwater biodiversity survey using environmental DNA

  MINAMOTO Toshifumi

  Green Science Seminar at Seishin Women's High School, Oct. 2018, Japanese, Seishin Women's High School, Domestic conference

  Public discourse


• Detection of genetic diversity using environmental DNA analysis

  TSUJI Sarsyki, MIYA Masaki, USHIO Masayuki, SATO Hirotoshi, MINAMOTO Toshifumi, MARUYAMA Atsushi, YAMANAKA Hiroki

  2018 Annual Meeting of the Ichthyological Society of Japan, Oct. 2018, Japanese, National Olympics Memorial Youth Center, Tokyo, Japan, Domestic conference

  Oral presentation


• Detection of genetic disturbance by colored medaka using environmental DNA method

  NAKAO Ryohei, KITAGAWA Tadao, MINAMOTO Toshifumi

  2018 Annual Meeting of the Ichthyological Society of Japan, Oct. 2018, Japanese, National Olympics Memorial Youth Center, Tokyo, Japan, Domestic conference

  Oral presentation


• Verification of environmental DNA detection accuracy of Acheilognathus longipinnis in Kiso River

  NAGAYAMA Shigeya, OHTA Munehiro, FUJITA Tomohiko, MINAMOTO Toshifumi, MORI Seiichi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Estimation of spatiotemporal distribution and migration timing of common shrimp in Lake Biwa

  WU Qianqian, KAWANO Ken, ISHIKAWA Toshiyuki, TSUJI Satsuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Monitoring of distribution of Ayu fish and cold water disease bacteria in Nagara and Ibi Rivers

  HIROSE Masae, MASAI Nanami, TSUNEGAWA Mitsuki, TSUCHIDA Kota, TAKAGI Masaki, YAOI Yuichi, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Detection of environmental DNA from bites of herbivorous insects

  KUDOH Aoi, MINAMOTO Toshifumi, YAMAMOTO Satoshi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• On the basis of environmental DNA analysis and distribution survey for rare or alien species

  MINAMOTO Toshifumi

  2018 Open Seminar of JAES Kansai Branch, Sep. 2018, Japanese, Osaka Garden Palace, Osaka, Japan, Domestic conference

  Public discourse


• Estimation of the breeding season of the Japanese giant salamander using environmental DNA analysis

  MORIMOTO Teppei, NAKAO Ryohei, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Attempt to investigate the ecology of fish and shellfish living in Lakes Shinji and Nakaumi using environmental DNA analysis

  TAKAHARA Teruhiko, YAMAGISHI Satoshi, TAGUCHI Junya, IKEBUCHI Takashi, TATEISHI Arata, OGATA Shigeki, INAOKA Yuuki, HATTORI Shinya, KUROSE Hiroto, FUJII Masato, DOI Hideyuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Estimating fish population abundance or biomass by integrating quantitative environmental DNA analysis and hydrodymamic modeling

  FUKAYA Keiichi, MURAKAMI Hiroaki, YOON Seokjin, MINAMI Kenji, OSADA Yutaka, YAMAMOTO Satoshi, MASUDA Reiji, KASAI Akihide, MIYASHITA Kazushi, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Protocol for environmental DNA survey

  MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Public discourse


• Detecting spawning migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus), using environmental DNA

  YATSUYANAGI Tetsu, KANBE Takashi, MIZUMOTO Hiroki, KOBAYASHI Yumi, KAMADA Shoko, NAMBA Satoko, NII Hisaya, MINAMOTO Toshifumi, SAKATA Masayuki K, ARAKI Hitoshi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Possibility of biomass estimation of coastal fish via environmental DNA

  MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Relationship between rare insect species and invasive alien species in tameike ponds clarified with environmental DNA analysis

  OGATA Shigeki, NISHIWAKI Atsuhiro, YAMAZOE Kanji, DOI Hideyuki, MINAMOTO Toshifumi, SUGAI Kyoko, TAKAHARA Teruhiko

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Comparison of detection trends with fish faunal survey by eDNA metabarcoding and literature data of rivers around Lake Biwa

  NAKAGAWA Hikaru, MINAMOTO Toshifumi, YAMAMOTO Satoshi, SATO Yukuto, SADO Tetsuya, MIYA Masaki

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Freshwater fish fauna around Rokko Montains clarified by environmental DNA metabarcoding

  NAKAO Ryohei, YAMAMOTO Satoshi, MIYA Masaki, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Effect of water temperature and biomass on release and decomposition of environmental DNA

  JO Toshiaki, MURAKAMI Hiroaki, YAMAMOTO Satoshi, MASUDA Reiji, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Introduction to environmental DNA

  MINAMOTO Toshifumi

  Shiga Water Environment Business Promotion Forum: Research and Technology Subcommittee, Sep. 2018, Japanese, Piazza Omi, Otsu, Japan, Domestic conference

  Public discourse


• Distribution pattern of Japanese black seabream inferred from environmental DNA

  SASANO Sachia, MURAKAMI Hiroaki, SUZUKI Keita, MINAMOTO Toshifumi, YAMASHITA Yo, MASUDA Reiji

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Development of environmental DNA metabarcoding detection assay for dragonfly species

  SAKATA K. Masayuki, UCHIDA Kei, SATO Hirotoshi, YAMANAKA Hiroki, NAKAO Ryohei, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Optimization of eDNA sampling in dam resoivers

  HAYAMI Kana, SAKATA Masayuki K, OKITSU Jiro, KATANO Izumi, MIYA Masaki, GOTOH Ryo, SATO Hirotoshi, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Investigation of inhibitory factors of environmental DNA detection of turtle species

  KAKUTA Aozora, HIGASHIGAKI Daisuke, MINAMOTO Toshifumi, DOI Hideyuki, KATANO Izumi

  The 22nd Annual Meeting of the Ecology and Civil Engineering Society, Sep. 2018, Japanese, Tokyo Institute of Technology, Tokyo, Japan, Domestic conference

  Poster presentation


• Investigation of inhibitory factors of environmental DNA detection of turtle

  KAKUTA Aozora, HIGASHIGAKI Daisuke, MINAMOTO Toshifumi, DOI Hideyuki, KATANO Izumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Evaluation of the fish distribution and their environmental DNA in a semi-closed bay using a three-dimensional tracer model

  YOON Seokjin, KASAI Akihide, MURAKAMI Hiroaki, MINAMI Kenji, MASUDA Reiji, MIYASHITA Kazushi, MINAMOTO Toshifumi, KONDOH Michio

  The First Annual Meeting of the eDNA Society, Sep. 2018, English, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Environmental DNA metabarcoding reveals fish species in Qingdao Underwater World

  CHEN Zhi, SONG Na, GAO Tianxiang, XU Shengyong, WU Qianqian, LI Di, CHEN Jianwei, ZHAO Linlin, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, English, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• An optional cost-effective method of extracting environmental DNA from filter membranes in large scale eDNA surveys

  CHEN Zhi, SONG Na, GAO Tianxiang, XU Shengyong, WU Qianqian, LI Di, CHEN Jianwei, ZHAO Linlin, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, English, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Detection of amphbiam species by using 16SrRNA metabarcoding of environmental DNA

  KAWATA Mone, KURABAYASHI Atsushi, Noerikanto Ramamonjisoa, NATSUHARA Yoshihiro, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conference

  Poster presentation


• Forensic investigation of rivers? examining underwater fauna with environmental DNA

  MINAMOTO Toshifumi

  2018 Hyogo prefecture freshwater fishermen workshop, Aug. 2018, Japanese, Hyogo Prefecture Kanzaki Branch Office, Domestic conference

  Public discourse


• The present and future situation of environmental DNA studies

  MINAMOTO Toshifumi

  2018 Open Seminar of JAES Hokkaido Branch, Aug. 2018, Japanese, Sapporo Education and Culture Hall, Sapporo, Japan, Domestic conference

  Public discourse


• The use of SNP markers in environmental DNA to detect intraspecific genetic variation

  UCHII Kimiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki

  2018 ESA Annual Meeting, Aug. 2018, English, Ernest N. Morial Convention Center, New Orleans, Louisiana, USA, International conference

  Poster presentation


• Examining PCR protocol for amplifying whole mitochondrial DNA of fish from environmental DNA

  YAMANAKA Hiroki, HIRAO Yohei, SATO Hirotoshi, MINAMOTO Toshifumi

  2018 ESA Annual Meeting, Aug. 2018, English, Ernest N. Morial Convention Center, New Orleans, Louisiana, USA, International conference

  Poster presentation


• Futuristic glasses looking into the world of aquatic organisms? ~ Environmental DNA

  MINAMOTO Toshifumi

  Science Gallery 2018, Jul. 2018, Japanese, International House, Osaka, Japan, Domestic conference

  Public discourse


• Clarifying underwater organisms by using environtal DNA

  MINAMOTO Toshifumi

  Seminar on environment in Mukogawa River, Jun. 2018, Japanese, Kobe City Hall, Domestic conference

  Public discourse


• Maximizing the potential of environmental DNA metabarcoding for fish detection in lentic ecosystem

  MINAMOTO Toshifumi, HAYAMI Kana, SAKATA Masayuki K, OKITSU Jiro, OKITSU Jiro, GOTOH Ryo O, SATO Hirotoshi, YAMANAKA Hiroki

  ASLO 2018 Summer Meeting, Jun. 2018, English, Victoria Conference Centre, British Columbia, Canada, International conference

  Poster presentation


• Detection of genetic diversity of a fish population using environmental DNA metabarcoding: Analysis strategies for eliminating sequence artifacts

  TSUJI Satsuki, MIYA Masaki, USHIO Masayuki, SATO Hirotoshi, SATO Yukuto, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 8th East Asian Federation of Ecological Societies International Congress, Apr. 2018, English, Nagoya University, Nagoya, Japan, International conference

  Poster presentation


• Can Salvelinus species coexist with rainbow trout in Hokkaido? ~Based on distribution data by environmental DNA ~

  HAYAMI Kana, SAKATA K. Masayuki, IMAMURA AKio, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Horizontal and vertical distribution of environmental DNA of Trachurus japonicus and Engraulis japonicus in Maizuru Bay

  MURAKAMI Hiroaki, YAMAMOTO Satoshi, MINAMOTO Toshifumi, MINAMI Kenji, MIYASHITA Kazushi, FUKAYA Keiichi, YUN Sukjin, KASAI Akihide, SAWADA Hideki, SUZUKI Keita, MASUDA Reiji, YAMASHITA Yoh, KONDOH Michio

  2018 Spring Meeting of Japanese Society of Fisheries Science, Mar. 2018, Japanese, Tokyo University of Marine Science and Technology, Domestic conference

  Oral presentation


• Genetic structure of common shrimp in Lake Biwa related to the life history polymorphism

  WU Qianqian, TAKAMI Yasuoki, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Investigation of basic technologies for environmental RNA analysis

  IKEDA Shizuya, HIRAO Yohei, TADA Satoru, SATO Hirotoshi, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Detection of breeding behavior of common carp using environmental RNA

  MAEGAWA Kazuya, MINAMOTO Toshifumi, YAMAMOTO Yoshihiko

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Improvement of environmental DNA detection assay for South East Asian liver fluke

  TOUGETANI Ayana, SATO Megumi, SATO Otake Marcello, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Current status and prospects of basic researches on environmental DNA analysis

  JO Toshiaki, MURAKAMI Hiroaki, MASYDA Reiji, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Detection of genetic diversity using environmental DNA Analysis: Application to wild Ayu population and its detectability

  TSUJI Satsuki, MIYA Masaki, USHIO Masayuki, SATO Hirotoshi, SATO Yukuto, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Oral presentation


• Factors regulating biodiversity of ponds - Case studies on environmental DNA analysis of dragonfly and fish species-

  SAKATA K. Masayuki, UCHIDA Kei, SATO Hirotoshi, YAMANAKA Hiroki, NAKAO Ryohei, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Establishment of environmental DNA assays for freshwater turtles

  KAWATA Mone, UENO Shintaro, KAMEZAKI Naoki, TANIGUCHI Mari, MINAMOTO TOSHIFUMI

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Estimation of breeding season of the Japanese Giant Salamander using environmental DNA analysis

  MORIMOTO Tepei, NAKAO Ryohei, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Fixed sites monitoring of Hida salamander using environmental DNA

  TOMITA Sei, KOHMATSU Yukihiro, MINAMOTO TOSHIFUMI

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Estimation of amphibian distribution by environmental DNA and analysis of environmental factors

  MISHIMA Tasuro, ONISHI Takeo, IWASAWA Atsushi, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• Coastal dependence of Acanthopagrus schlegelii indestigated by environmental DNA and distribution characteristics of egg-laying and larval stage

  SASANO Sachia, MURAKAMI Hiroaki, SUZUKI Keita, MINAMOTO Toshifumi, YAMASHITA Yoh, MASUDA Reiji

  2018 Spring Meeting of Japanese Society of Fisheries Science, Mar. 2018, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conference

  Oral presentation


• Exploring the dynamics of host and pathogenic organisms using environmental DNA

  UCHII Kimiko, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Attempt to estimate resources of Corbicula japonica in Lake Shinji using environmental DNA

  TAKAHARA Teruhiko, TAGUCHI Junya, IKEBUCHI Takashi, UCHIDA Hiroshi, ISHIDA Kenji, YAMAGISHI Satoshi, OGATA Shigeki, DOI Hideyuki, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Oral presentation


• Investigation of fish fauna around Mt. Rokko using environmental DNA metabarcoding

  NAKAO Ryohei, YAMAMOTO Satoshi, MIYA Masaki, MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Oral presentation


• Identifying breeding seasons and locations using environmental DNA

  MINAMOTO Toshifumi

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Investigation of PCR conditions for acquisition of full length mitochondrial DNA sequence from environmental DNA samples

  HIRAO Yohei, SATO Hirotoshi, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conference

  Poster presentation


• A research on environmental DNA of Leptospira in north Okinawa

  MIZUYAMA Masaru, SATO Yukuto, SATO Megumi, MINAMOTO Toshifumi, CLAUDIA Toma

  The 55th Leptospira Symposium, Mar. 2018, Japanese, Kyushu University, Fukuoka, Japan, Domestic conference

  Oral presentation


• The impact of biodiversity in river on valuation of urban ecosystem services: the case of Hanshin region using eDNA and the residents' life satisfaction data

  AOSHIMA Ippei, NAKAO Ryohei, MINAMOTO Toshifumi, USHIMARU Atushi, SATO Masayuki

  The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, English, Sapporo Convention Center, Sapporo, Japan, International conference

  Oral presentation


• Environmental DNA: next generation monitoring method for aquatic biodiversity

  MINAMOTO Toshifumi

  11th International Symposium Exploring the Global Sustainability, Mar. 2018, English, Graduate School of Human Development and Environment, International conference

  [Invited]

  Invited oral presentation


• Suvey for turtles using environmental DNA

  MINAMOTO Toshifumi

  Symposium "Considering the freshwater organisms in Okayama: mainly on turtles", Feb. 2018, Japanese, Okayama University of Science, Domestic conference

  [Invited]

  Invited oral presentation


• Underwater biodiversity survey using environmental DNA

  MINAMOTO Toshifumi

  Green Science Seminar at Seishin Women's High School, Jan. 2018, Japanese, Seishin Women's High School, Domestic conference

  Public discourse


• Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, by combining eDNA and conventional surveys

  MINAMOTO Toshifumi, SAKATA K. Masayuki, MAKI Nobutaka, SUGIYAMA Hideki

  Ecology Across Borders: Joint Annual Meeting 2017, Dec. 2017, English, ICC Ghent, Belgium, International conference

  Poster presentation


• Fish larvae inside? Indirect assessment of fish larvae presence in host mussels using environmental DNA analysis.

  YAMANAKA Hiroki, YONEKURA Ryuji, KOMATSU Fumiya, KAKAMU Hiroto, TAKINO Fumiya, TADA Satoru, YAMAMOTO Yoshihiko, MINAMOTO Toshifumi

  Ecology Across Borders: Joint Annual Meeting 2017, Dec. 2017, English, ICC Ghent, Belgium, International conference

  Poster presentation


• Monitoring of rare species using environmental DNA analysis

  MINAMOTO Toshifumi

  Technical Seminar, Society of Environmental Conservation Engineering, Nov. 2017, Japanese, Osaka Kyoiku University, Osaka, Japan, Domestic conference

  Public discourse


• Southeast Asian liver fluke in Cambodia: identification of a new endemic area

  MIYAMOTO Kazuko, MATSUDA Hajime, CHIGUSA Yuichi, KIRINOKI Masashi, SATO Marcello, MINAMOTO Toshifumi

  Global Health 2017, Nov. 2017, Japanese, University of Tokyo, Tokyo, Japan, Domestic conference

  Oral presentation


• Development of distribution survey method for macro-organisms using environmental DNA

  MINAMOTO Toshifumi

  The 149th RIHN Seminar "Observation, analysis and theory in ecology for next generations -What we have achieved in global environment studies-", Nov. 2017, English, Research Institute for Humanity and Nature, Kyoto, Japan, International conference

  [Invited]

  Invited oral presentation


• The application of eDNA technique for the monitoring of marine fish species

  KONDOH Michio, ARAKI Hitoshi, KASAI Akihide, MASUDA Reiji, MINAMOTO Toshifumi, MIYA Masaki

  The 33rd Annual Meeting of Society for Population Ecology, Oct. 2017, English, Kyusyu University, Fukuoka, Japan, Domestic conference

  Poster presentation


• Identification of a breeding habitat of a critically endangered fish species, Acheilognathus typus, in the main stream of the Omono River

  SAKATA Masayuki, MAKI Nobutaka, SUGIYAMA Hideki, MINAMOTO Toshifumi

  The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conference

  Oral presentation


• Application of environmental DNA analysis in the Lake Biwa and connected lakes

  WU Qianqian, KAWANO Ken, UEHARA Yoshitoshi, OKUDA Noboru, TSUJI Satsuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Relationship between the coverage of Egeria densa and eDNA concentration in Saba and Takatsu rivers

  AKAMATSU Yoshihisa, DOI Hideyuki, GOTO Masuji, KOMURO Takashi, INUI Ryutei, NAGANO Mariko, MINAMOTO Toshifumi

  ELR2017 Nagoya, Sep. 2017, Japanese, Nagoya University, Nagoya, Japan, Domestic conference

  Poster presentation


• Confirmation of the effectiveness of eDNA analysis for the survey of rare species - detection of an unknown breeding site of Acheilognathus typus

  MAKI Nobutaka, SAKATA Masayuki K, SUGIYAMA Hideki, MINAMOTO Toshifumi, TOKI Kimihito

  2017 Annual Meeting of Japanese Society of Civil Engineers, Sep. 2017, Japanese, Kyushu University, Fukuoka, Japan, Domestic conference

  Oral presentation


• What is environmental DNA analysis?

  MINAMOTO Toshifumi

  The 82nd Annual Meeting of Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conference

  Public discourse


• Monitoring method for Acheilognathus longipinnis using environmental DNA

  KODUKI Sayuko, YAMAMOTO Yoshihiko, MINAMOTO Toshifumi, WATANABE Takeshi, UEHARA Kazuhiko

  2017 Annual Meeting of Japanese Society of Civil Engineers, Sep. 2017, Japanese, Kyushu University, Fukuoka, Japan, Domestic conference

  Oral presentation


• Environmental DNA survey of alien-species distribution in ponds - application for Trachemys scripta and consideration of PCR inhibition

  SOUMA Rio, MINAMOTO Tishifumi, DOI Hideyuki, KATANO Izumi

  ELR2017 Nagoya, Sep. 2017, Japanese, Nagoya University, Nagoya, Japan, Domestic conference

  Poster presentation


• Environmental DNA analysis using nuclear DNA markers

  UCHII Kimiko, DOI Hideyuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conference

  Oral presentation


• Are environmental DNA methods effective for species survey in rivers and reservoirs?

  MINAMOTO Toshifumi

  ELR2017 Nagoya, Sep. 2017, Japanese, Nagoya University, Nagoya, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Comparison of the accuracy of visual and environmental DNA surveys for the turtle species, Trachemys scripta: Toward constructing a potential map for prediction of invasion into ponds

  KAKUDA Aozora, TOUGAKI Daisuke, SOUMA Rio, MINAMOTO Toshifumi, DOI Hideyuki, KATANO Izumi

  The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conference

  Poster presentation


• Environmental DNA and its application in opisthorchiasis and schistosomiasis in endemic areas

  SATO Marcello Otake, RAFALIMANANTSOA-SOLOFONIAINA Armand, PONGVONGSA Tienkam, MINAMOTO Toshifumi, WAIKAGUL Jitra, KAWAI Satoru, CHIGUSA Yuichi

  The 11th Helminth Research Meeting, Sep. 2017, English, Iojima, Nagasaki, Japan, Domestic conference

  Oral presentation


• Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing

  USHIO Masayuki, MURAKAMI Hiroaki, MASUDA Reiji, SADO Tetsuya, MIYA Masaki, SAKURAI Sho, YAMANAKA Hiroki, MINAMOTO Toshifumi, KONDOH Michio

  2017 ESA Annual Meeting, Aug. 2017, English, Oregon Convention Center, Portland, Oregon, U.S.A., International conference

  Oral presentation


• Longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA

  MINAMOTO Toshifumi, JO Toshiaki, MURAKAMI Hiroaki, MASUDA Reiji, SAKATA Masayuki, SAKATA Masayuki

  2017 ESA Annual Meeting, Aug. 2017, English, Oregon Convention Center, Portland, Oregon, U.S.A., International conference

  Poster presentation


• Environmental DNA survey methods: Water sampling methods using an unmanned aerial vehicle

  DOI Hideyuki, AKAMATSU Yoshihisa, WATANABE Yutaka, GOTO Masuji, INUI Ryutei, KATANO Izumi, NAGANO Mariko, TAKAHARA Teruhiko, MINAMOTO Toshifumi

  2017 ESA Annual Meeting, Aug. 2017, English, Oregon Convention Center, Portland, Oregon, U.S.A., International conference

  Poster presentation


• Environmental DNA reflects the spatial and temporal distribution of common prawn, Palaemon paucidens, in Lake Biwa

  WU Qianqian, ISHIKAWA Toshiyuki, TSUJI Satsuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  International Congress of Ecology 2017, Aug. 2017, English, China National Convention Center, Beijing, China, International conference

  Poster presentation


• Biological survey by only collecting water: Development of environmental DNA technology to understand the type and quantity of aquatic organisms

  MINAMOTO Toshifumi

  Invitation letter to the near future - messages from nice step researcher 2016, Jul. 2017, Japanese, Japanese Ministry of Education, Culture, Sports, Science and Technology (Tokyo, Japan), Domestic conference

  Public discourse


• Investigate the fish in the Kobe Port using "environmental DNA"!

  MINAMOTO Toshifumi

  Let's learn! 'Sea Science'!, Jul. 2017, Japanese, Kobe City Industry Promotion Center (Kobe, Japan), Domestic conference

  Public discourse


• Investigating underwater organisms using DNA

  MINAMOTO Toshifiumi

  Special Seminar at HYOGO KANKYO TAIKENKAN, Jul. 2017, Japanese, HYOGO KANKYO TAIKENKAN（Sayo Town, Hyogo, Japan), Domestic conference

  Public discourse


• What is environmental DNA?

  MINAMOTO Toshifumi

  The 47th Symposium on the Sub-arctic Fisheries and Oceanography, Jun. 2017, Japanese, Hokkaido University, Sapporo, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Species-specific detection of vertebrates and invertebrates by eDNA analysis

  MINAMTO Toshifumi

  2017 International Workshop on Environmental Genomics, Jun. 2017, English, St. Johns, Canada, International conference

  [Invited]

  Invited oral presentation


• Search for habitats of threatened species using environmental DNA

  MINAMOTO Toshifumi

  The Japan Society for Analytical Chemistry, 77th Meeting, May 2017, Japanese, Ryukuko University, Kyoto, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• On the survey methods for endangered or alien species using environmental DNA

  MINAMOTO Toshifumi

  The 67th Hiyoshi Seminar, May 2017, Japanese, Hiyoshi Co. LTD., Ohmi-Hachiman, Shiga, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Numerical simulation on distribution of environmental DNA of Japanese jack mackerel (Trachurus japonicus) in a semi-closed bay

  YOON Seokjin, KASAI Aikihide, YAMAMOTO Satoshi, MINAMOTO Toshifumi, MINAMI Kenji, MIYASHITA Kazushi, MASUDA Reiji, KONDOH Michio

  2017 The Korean Society of Fisheries and Aquatic Sciences Spring Meeting, May 2017, English, Korea, International conference

  Oral presentation


• Organism is found by examining the water ~the wonders of environmental DNA~

  MINAMOTO Toshifumi

  The 202nd Yomiuri Techno Forum, Apr. 2017, Japanese, Nippon Press Center, Tokyo, Japan, Domestic conference

  Public discourse


• Rediscovery of a critically endangered freshwater fish;Acheilognathus typus;in the main stream of Omono River

  SAKATA Masayuki, MAKI Nobutaka, SUGIYAMA Hideki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Detection of environment DNA of rare fish species in rivers in Sasayama City;Hyogo Prefecture

  KAWANO Ken, KIYONO Mieko, NIWA Hideyuki, SHINOTANI Kazuhiko, TAI Akito, SAKATA Masayuki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Detection of non-migrating ecotype of common shrimp in Lake Biwa ~ Analysis using environmental DNA

  WU Qianqian, UEHARA Yoshitoshi, OKUDA Noboru, TSUJI Satsuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conference

  Poster presentation


• Quantitative monitoring of environmental DNA from multiple fish species by using mass-sequencing

  USHIO Masayuki, MURAKAMI Hiroaki, MASUDA Reiji, SADO Tetsuya, MIYA Masaki, SAKURAI Sho, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Detection of environmental DNA of Japanese jack mackerel from sediments: aquarium experiments and field surveys

  OGATA Mizuki, MASUDA Reiji, MURAKAMI Hiroaki, YODEN Takaaki, SAWADA Hideki, YAMASHITA Yoh, MINAMOTO Toshifumi

  2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conference

  Oral presentation


• Biological survey methods using environmental DNA information

  MINAMOTO Toshifumi

  The 122nd Meeting of Japanese Society of Animal Science, Mar. 2017, Japanese, Kobe University, Kobe, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Evaluation of a species distribution model based on environmental DNA analysis - An case study of Japanese giant salamander (Andrial japonicus) -

  HIDAKA Shunsuke, KATSUHARA R. Koki, TOMITA Sei, USHIMARU Atushi, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Progress of the biodiversity survey by environmental DNA analysis and its applicability

  MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Application of environmental DNA method to the ecology of infectious diseases: detection of schistosomes

  WATANABE Marina, SATO O. Marcello, KIRINOKI Masashi, SATO Megumi, IKEDA Sumire, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Evaluation of intraspecific genetic variation using environmental DNA: a new method for removing false haplotypes derived from NGS analysis

  TSUJI Satsuki, MIYA Masaki, USHIO Masayuki, SATO Yukuto, YAMAMOTO Satoshi, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Development of jellyfish control measure using environmental DNA: seasonal monitoring and detection from polyp

  YODEN Takaaki, MASUDA Reiji, MURAKAMI Hiroaki, OGATA Mizuki, YAMASHITA Yoh, MINAMOTO Toshifumi

  2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conference

  Oral presentation


• Environments DNA meta barcoding reveals seasonal change of fish community in a river

  YAMAMOTO Satoshi, MIYA Masaki, SADO Tetsuya, NAKAGAWA Hikaru, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Fish fauna survey by environmental DNA metabarcoding - a case study of small rivers in Rokko Mountains -

  NAKAO Ryohei, YAMAMOTO Satoshi, MIYA Masaki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Effect of water temperature on degradation and size fraction of environmental DNA

  JO Toshiaki, MURAKAMI Hiroaki, YODEN Takaya, OGATA Mizuki, YAMAMOTO Satoshi, MASUDA Reiji, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Genetic structure analysis of hybrid populations using environmental DNA analysis with nuclear DNA markers

  UCHII Kimiko, DOI HideyukiI, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Relationship between population density and environmental DNA concentration of Lateolabrax japonicus fry in different habitats

  MURAKAMI Hiroaki, SOGABE Kyosei, SUZUKI Yuto, MINAMOTO Toshifumi, KASAHI Akihide, SUZUKI Keita, YAMASITA Yoh, MASUDA Reiji

  2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Domestic conference

  Oral presentation


• Environment DNA detection of Hynobius spp. using universal primers

  TOMITA Sei, KOHMATSU Yukihiro, YAMANAKA Hiroki, NAGANO Masahiro, SATO Takuya, TAKAHARA Teruhiko, SAWADA Hayato, KATSUHARA R. Koki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Optimization of environmental DNA detection method in dam reservoirs

  IKEDA Saki, OKITSU Jiro, ICHIYANAGI Hidetaka, KATANO Izumi, NAKAI Katsuki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Investigation of optimal biomass estimation method using environmental DNA using Hemigrammocypris rasborella as a model species

  TAKAHARA Teruhiko, MATSUMOTO Munehiro, MINAMOTO Toshifumi, DOI Hideyuki

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• Environmental DNA as an ecological tool for salmonid fish distribution

  ARAKI Hitodhi, KANBE Takashi, MIZUMOTO Hiroki, TAKAHARA Teruhiko, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, English, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Spatio-temporal distribution of common prawn in Lake Biwa clarified by eDNA analysis

  WU Qianqian, ISHIKAWA Toshiyuki, TSUJI Satsuki, YAMANAKA Hiroki, TAKAMI Yasuoki, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conference

  Poster presentation


• A large scale study of aquatic plants according to eDNA analysis

  FUJIWARA Ayaka, UCHIDA Kei, SATO Hirotoshi, HAYASHI Norio, MINAMOTO Toshifumi

  The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, English, Waseda University, Tokyo, Japan, Domestic conference

  Oral presentation


• Development of a novel survey method for aquatic organisms using environmental DNA

  MINAMOTO Toshifumi

  The 38st Green Science Seminar, Jan. 2017, Japanese, Fukuyama University, Fukuyama, Japan, Domestic conference

  Public discourse


• A new biological survey method "environmental DNA analysis"

  MINAMOTO Toshifumi

  2016 Natural Science Lecture, Dec. 2016, Japanese, Agenda 21, Citizen's Environmental Conference in Toyonaka, Toyonaka City Central Community Center, Toyonaka, Osaka, Japan, Domestic conference

  Public discourse


• Numerical experiment on environmental DNA distribution ofJapanese jack mackerel in Maizuru Bay

  YOON Seokjin, KASAI Akihide, YAMAMOTO Satoshi, MINAMOTO Toshifumi, MINAMI Kenji, MIYASHITA Kazushi, MASUDA Reiji, KONDOH Michio

  2016 Annual Meeting of Japanese Society of Fisheries Oceanography, Nov. 2016, Japanese, Tokyo University of Marine Science and Technology, Domestic conference

  Oral presentation


• Detection of a semi-aquatic mammal nutria by environmental DNA analysis

  YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Poster presentation


• Metabarcoding of fish DNA using environmental DNA in sediments

  SAKATA Masayuki, YAMAMOTO Satoshi, MIYA Masaki, MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Detection of Opisthorchis viverrini DNA from Environmental Water Sample by eDNA Methods

  SATO Megumi, HASHIZUME Hiroki, SATO Otake Marcello, YOONUAN Tippayarat, SURAPOL Sanguankiat, IKEDA Sumire, PONGVONGSA Tiengkham, MOJI Kazuhiko, MINAMOTO Toshifumi

  The 57th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2016, Japanese, Hitotsubashi University, Tokyo, Japan, Domestic conference

  Poster presentation


• Current status and issues of environmental DNA analysis

  MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• The use of SNP markers in environmental DNA researches

  UCHII Kimiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Habitat survey of Acheilognathus longipinnis in Yodo River using environmental DNA

  YAMAMOTO Yoshihiko, KONDOH Mio, NAITOH Kaoru, UEHARA Kazuhiko, KODUKI Sayoko, WATANABE Takeshi, TSURUTA Tetsuya, INANAMI Riko, MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• A novel ecosystem survey method using environmental DNA and its applicability to water environment management

  MINAMOTO Toshifumi

  The 10th Nagoya presentation meeting of the River Foundation, Nov. 2016, Japanese, Wink Aichi, Nagoya, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Distribution survey of mountain stream inhabiting amphibians Onychodactylus japonicus using environmental DNA

  KATANO Izumi, HARADA Ken, SOUMA Rio, SAKATA Yusuke, MINAMOTO Toshifumi, DOI Hideyuki

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Evaluation of the species distribution model for Japanese giant salamander based on environmental DNA analysis

  HIDAKA Shunsuke, KATSUHARA R. Koki, TOMITA Sei, USHIMARU Atsushi, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Estimation of fish distribution and biomass: comparison of real-time PCR and digital PCR

  DOI Hideyuki, UCHII Kimiko, TAKAHARA Teruhiko, MATSUHASHI Saeko, YAMANAKA Hiroki, MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Environmental DNA detection of Hynobisu species using universal primers

  TOMITA Sei, KOHMATSU Yukihiro, YAMANAKA Hiroki, NAGANO Masahiro, SATO Takuya, TAKAHARA Teruhiko, SAWADA Shun, MINAMOTO Toshifumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Habitat survey for Trachemys scripta: application of environmental DNA technology and examination for PCR inhibition factor

  SOUMA Rio, MINAMOTO Toshifumi, DOI Hideyuki, KATANO Izumi

  The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conference

  Oral presentation


• Environmental DNA anlysis: introduction and some examples of target species detection

  MINAMOTO Toshifumi

  The 5th Japan-Taiwan Ecology Workshop, Nov. 2016, English, Ryukoku University, Kyoto, Japan, International conference

  [Invited]

  Invited oral presentation


• Application of environmental DNA to detect Opisthorchis viverrini in water sample in Savhannakhet, Laos

  SATO Megumi, HASHIZUME Hiroki, SATO Marcello Otake, IKEDA Sumire, YOONUAN Tippayarat, SANGUANKIAT Surapol, PONGVONGSA Tiengkham, MOJI Kazuhiko, MINAMOTO Toshifumi

  The 10th National Health Research Forum, Laos, Oct. 2016, English, Savannakhet, Laos, International conference

  Poster presentation


• Estimating the distribution of Acheilognathus longipinnis in Yodo River by eDNA anakysis

  KODUKI Sayoko, INANAMI Riko, MINAMOTO Toshifumi, UEHARA Kazuhiko, YAMAMOTO Yoshihiko

  Annual Meeting of Japan Society of Civil Engineers, Sep. 2016, Japanese, Sendai City, Miyagi, Japan, Domestic conference

  Oral presentation


• Trial of monitoring of natural monument, Acheilognathus longipinnis using eDNA

  YAMAMOTO Yoshihiko, NAITOH Kaoru, UEHARA Kazuhiko, TSURUTA Tetsuya, KODUKI Sayoko, WATANABE Takeshi, INANAMI Riko, MINAMOTO Toshifumi

  2016 Annual Meeting of The Ichthyological Society Japan, Sep. 2016, Japanese, Gifu City, Gifu, Japan (Gifu University), Domestic conference

  Poster presentation


• Year-round migration history of ayu sweetfish assessed by environmental DNA analysis: Implications for conservation and fisheries management

  YAMANAKA Hiroki, SAKURAI Sho, YAMAMOTO Daisuke, YAMAMOTO Toshiya, MINAMOTO Toshifumi

  Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conference

  Poster presentation


• Use of environmental DNA to survey the distributions of the salamander and fish species

  KATANO Izumi, HARADA Kei, SOUMA Rio, SAKATA Yusuke, DOI Hideyuki, MINAMOTO Toshifumi

  Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conference

  Poster presentation


• Use of Environmental DNA for detection of fish-water borne zoonosis and perspectives to the application of the technology in Madagascar

  SATO Marcello Otake, SATO Megumi, MINAMOTO Toshifumi

  24th Molecular Parasitology Workshop / 14th Molecular Parasitology and Malaria Research Forum, Aug. 2016, English, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan, Domestic conference

  Oral presentation


• Estimating the distribution of the Japanese giant salamander (Andrias japonicus) by ecological niche modeling based on environmental DNA detection

  MINAMOTO Toshifumi, HIDAKA Shunsuke, KATSUHARA Koki, TOMITA Sei, YAMAMOTO Satoshi, USHIMARU Atushi

  Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conference

  Poster presentation


• Environmental DNA method for estimating fish abundance and biomass

  DOI Hideyuki, INUI Ryutei, UCHII Kimiko, AKAMATSU Yoshihisa, KANNO Kazuki, MATSUHASHI Saeko, TAKAHARA Teruhiko, YAMANAKA Hiroki, MINAMOTO Toshifumi

  Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conference

  Oral presentation


• Application of environmental DNA to analysis of mitochondrial haplotypes of fish

  TSUJI Satsuki, YAMANAKA Hiroki, MIYA Masaki, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conference

  Poster presentation


• Environmental DNA analysis provides "snapshots" of species distribution

  MINAMOTO Toshifumi

  International symposium on alternative stable states: a unifying concept in global change ecology, Jul. 2016, English, Kyoto University, Kyoto, Japan, International conference

  [Invited]

  Invited oral presentation


• Application of environmental DNA analysis to ecological surveys

  MINAMOTO Toshifumi

  International Symposium of Probiotics Application on Aquaculture, Jul. 2016, English, National Kaohsiung Marine University, Kaohsiung, Taiwan, International conference

  [Invited]

  Invited oral presentation


• Understanding the distribution of underwater species using environmental DNA

  MINAMOTO Toshifumi

  35th Special Lecture of Hyogo Biotechnology Study Group, Jun. 2016, Japanese, Kobe University, Kobe, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• Underwater biodiversity revealed by environmental DNA analysis

  MINAMOTO Toshifumi

  Kyoto Sangyo University BioForum, Jun. 2016, Japanese, Kyoto Sangyo University, Kyoto, Japan, Domestic conference

  Public discourse


• Environmental DNA

  MINAMOTO Toshifumi

  The 26th lecture on animal survey methods for environmental assessments, Jun. 2016, Japanese, Osaka, Japan, Domestic conference

  Public discourse


• Analysis on relationship between fish distribution and environmental DNA in a semi -closed bay using a three dimensional regional circulation model

  YOON Seokjin, KASAI Akihide, YAMAMOTO Satoshi, MINAMI Kenji, MINAMOTO Toshifumi, MIYASHITA Kazushi, MASUDA Reiji, KONDOH Michio

  2016 Korean Society of Oceanology Spring Meeting, May 2016, English, Busan, Korea, Domestic conference

  Oral presentation


• Environmental DNA detection from sediments

  SAKATA Masayuki, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• A large survey of Japanese giant salamander (Andrias japonicus) by using environmental DNA

  HIDAKA Shunsuke, KATSUHARA Kohki, TOMITA Sei, USHIMARU Atushi, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• Detection of mitochondria DNA haplotype in ayu (Plecoglossus altivelis) by environmental DNA analysis

  TSUJI Satsuki, MIYA Masaki, SATO Yukuto, YAMAMOTO Satoshi, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• What kind of information to be obtained by the environmental DNA analysis?

  MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Detection of liver fluke Opisthorchis viverrini by environmental DNA analysis

  HASHIZUME Hiroki, SATO Megumi, Marcello Otake SATO, Tippayarat, YOONUAN, Surapol SANGUANKIAT, Tiengkham, PONGVONGSA, MOJI Kazuhiko, MINAMOTO Toshifumi

  The 85th Annual Meeting of the Japanese Society of Parasitology, Mar. 2016, Japanese, Miyazaki Citizen's Plaza, Miyazaki, Japan, Domestic conference

  Oral presentation


• Application of environmental DNA analysis for ecology of infectious diseases: the detection of liver fluke Opisthorchis viverrini

  HASHIZUME Hiroki, SATO Megumi, Marcello Otake SATO, Tippayarat, YOONUAN, Surapol SANGUANKIAT, Tiengkham, PONGVONGSA, MOJI Kazuhiko, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Practices and questions of environmental DNA research: challenges for the frontier of ecology

  ARAKI Hitoshi, KANBE Takashi, KAMADA Shouko, MINAMOTO Toshifumi, SATO Yukuto, MIYA Masaki

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Application of environmental DNA analysis for sub-merged plants: tests of survey methods and evaluation of the usefulness

  MATSUHASHI Saeko, MINAMOTO Toshifumi, FUJIWARA Ayaka, WATANABE Sonoko, YAMANAKA Hiroki, DOI Hideyuki

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• Large scale fish fauna survey in the rivers using "MiFish", the universal primers for environmental DNA analysis

  NAKAGAWA Hikaru, MINAMOTO Toshifumi, YAMAMOTO Satoshi, SATO Yukuto, MIYA Masaki

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• Biomass estimation of ayu (Peloglossus altivelis) by environmental DNA analysis in the logic environments

  TAKAHARA Teruhiko, MORISHITA Daigo, DOI Hideyuki, YAMANAKA Hiroki, MINAMOTO Toshifumi, KAWADA Gyou

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Fish fauna monitoring in Yodo River by using environmental DNA metabarcoding

  INANAMI Riko, YAMAMOTO Yoshihiko, KONDO Mio, UEHARA Kazuhiko, SATO Yukuto, MIYA Masaki, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• The difference of degradation rate between different lengths of environmental DNA

  JO Toshiaki, MURAKAMI Hiroaki, SAKATA Masayuki, MASUDA Reiji, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• Verification of the environmental DNA dispersion process in a coastal area

  MURAKAMI Hiroaki, YOON Seokjin, KASAI Akihide, MINAMOTO Toshifumi, YAMAMOTO Satoshi, SATAKA Masayuki, Horiuchi Tomoya, Sawed Hideki, MASUDA Reiji

  The 2016 Spring Meeting of Japanese Society of Fisheries Science, Mar. 2016, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conference

  Oral presentation


• Environmental DNA survey using multiplex PCR for endemic and exotic fish species in the ponds in Hyogo Prefecture

  FUKUOKA Arisa, USHIMARU Atushi, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Estimation of the distribution and biomass by digital PCR

  DOI Hideyuki, UCHII Kimiko, TAKAHARA Teruhiko, MATSUHASHI Saeko, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Detection of environmental DNA of Hynobius species using universal primers

  TOMITA Sei, KOHMATSU Yukihiro, YAMANAKA Hiroki, NAGANO Masahiro, MINAMOTO Toshifumi

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  Poster presentation


• Estimation of genotype frequencies by quantitative SNP analysis

  UCHII Kimiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki

  The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Spatial and temporal distribution surveys for marine jellyfish using environmental DNA

  MINAMOTO Toshifumi, FUKUDA Miho, KATSUHARA Kohki, FUJIWARA Ayaka, HIDAKA Shunsuke, YAMAMOTO Satoshi, MASUDA Reiji

  2015 Annual Meeting British Ecological Society, Dec. 2015, English, Edinburgh, UK., International conference

  Poster presentation


• eDNA based non-invasive method to analyze mitochondrial haplotype of fish

  TSUJI Satsuki, YAMAMOTO Satoshi, MINAMOTO Toshifumi, YAMANAKA Hiroki

  2015 Annual Meeting British Ecological Society, Dec. 2015, English, Edinburgh, UK, International conference

  Poster presentation


• 新たな環境DNAマーカーとしての核DNAの利用

  MINAMOTO Toshifumi, TAKAHARA Teruhiko, KITAYOSHI Takumi, TSUJI Satsuki, YAMANAKA Hiroki, UCHII Kimiko, DOI Hideyuki

  日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference

  Oral presentation


• 環境影響評価における新しい調査手法の試み- 環境DNAを用いたニホンザリガニ生息場の推定-

  IKEDA Kousuke, DOI Hideyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi, SUZUKI Tohru, KOIZUMI Itsuro, TANAKA Kazunori, NUNOKAWA Masanori

  日本土木学会第70回年次学術講演会, Sep. 2015, Japanese, 岡山県岡山市(岡山大学), Domestic conference

  Oral presentation


• 環境影響評価における新しい調査手法の試み-環境DNAを用いたイタセンパラ生息の推定-

  KOUZUKI Sayoko, WATANABE Takeshi, MINAMOTO Toshifumi, UEHARA Kazuhiko, YAMAMOTO Yoshihiko

  日本土木学会第70回年次学術講演会, Sep. 2015, Japanese, 岡山県岡山市(岡山大学), Domestic conference

  Oral presentation


• 環境DNA分析によるコイの季節移動の推定

  UCHII Kimiko, DOI Hideyuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference

  Oral presentation


• 環境DNA手法の新展開:魚類個体群の遺伝的多様性評価の試み

  TSUJI Satsuki, YAMANAKA Hiroki, MINAMOTO Toshifumi, YAMAMOTO Satoshi

  日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference

  Poster presentation


• デジタルPCRを用いた環境DNAによる生物量・生物分布推定

  DOI Hiedeyuki, UCHII Kimiko, TAKAHARA Teruhiko, MATSUHASHI Saeko, YAMANAKA Hiroki, MINAMOTO Toshifumi

  日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference

  Poster presentation


• ため池の環境DNA量と生物量の比較:池干し時の採捕調査による検証

  SOUMA Rio, KATANO Izumi, MINAMOTO Toshifumi, TAKAHARA Teruhiko, DOI Hideyuki

  日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference

  Oral presentation


• 水を汲むだけで生物分布がわかる？環境DNA 手法の開発と実践

  MINAMOTO Toshifumi

  第30回環境計量技術事例発表会, Jul. 2015, Japanese, 兵庫県計量協会環境計量証明部会, Domestic conference

  Public discourse


• Environmental DNA Assessment of Rare Endemic Species and Closely Related Exotic Species: A Case Study of Giant Salamanders in Japan

  MINAMOTO Toshifumi, FUKUKMOTO Sou, HIDAKA Shunsuke, USHIMARU Atushi

  2015 Society of Wetland Scientist Annual Meeting, Jun. 2015, English, Providence, Rhode Island, USA, International conference

  [Invited]

  Nominated symposium


• 環境DNA分析を用いた希少在来種と近縁外来種の流域スケール調査：京都府桂川のオオサンショウウオを例に

  FUKUMOTO Sou, USHIMARU Atushi, MINAMOTO Toshifumi

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Oral presentation


• 環境DNA手法を用いた在来および外来コイの生息地利用の推定

  UCHII Kimiko, DOI Hideyuki, YAMANAKA Hiroki, MINAMOTO Toshifumi

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Oral presentation


• 環境DNA手法の新たな展開〜沈水植物の検出法の開発〜

  FUJIWARA Ayaka, MATSUHASHI Saeko, DOI Hideyuki, MINAMOTO TOSHIFUMI

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Poster presentation


• 環境DNA技術を利用した沈水植物の生物量推定

  MATSUHASHI Saeko, MINAMOTO Toshifumi, FUJIWARA Ayaka, WATANABE Sonoko, DOI Hideyuki

  日本生態学会第62回全国大会, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• 環境DNA技術を用いた，ため池の生物分布調査：池干しによる採捕調査との比較

  SOUMA Rio, KATANO Izumi, MINAMOTO Toshifumi, TAKAHARA Teruhiko, DOI Hideyuki

  日本陸水学会近畿支部会第26回研究発表会, Mar. 2015, Japanese, 京都府京都市, Domestic conference

  Oral presentation


• 環境DNAを用いた魚類モニタリング

  MINAMOTO Toshifumi, KONDOH Michio, KASAI Akihide

  日本海洋学会 2015年度春季大会 沿岸海洋シンポジウム「沿岸海洋学における観測研究の最前線I」, Mar. 2015, Japanese, 東京都港区, Domestic conference

  [Invited]

  Nominated symposium


• 環境DNAを用いたクラゲ類の分布調査～舞鶴湾のアカクラゲを例に～

  FUKUDA Miho, MASUDA Reiji, FUJIWARA Ayaka, YAMAMOTO Satoshi, MINAMOTO Toshifumi

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Poster presentation


• 環境DNAの正体を探る：挙動の分析と観察によるアプローチ

  HASHIZUME Hiroki, MINAMOTO Toshifumi

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Poster presentation


• 環境DNAとマルチプレックスPCRを用いた複数魚種の同時検出

  FUKUOKA Arisa, USHIMARU Atushi, MINAMOTO Toshifumi

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Poster presentation


• eDNA解析による舞鶴湾におけるマアジ資源量の推定

  YAMAMOTO Satoshi, MINAMI Kenji, FUKAYA Keiichi, MASUDA Reiji, MIYASHITA Kazushi, KONDIH Michio, MINAMOTO Toshifumi

  日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference

  Poster presentation


• Assessment of the effect of artificial obstructions on fish migration in a river using environmental DNA

  YAMANAKA Hiroki, MINAMOTO Toshifumi

  Joint 2014 Annual Meeting British Ecological Society and Société Française d’Ecologie, Dec. 2014, English, Lille, France, International conference

  Oral presentation


• A basin-scale application of environmental DNA assessment for rare endemic and exotic giant salamander species in Japan

  MINAMOTO Toshifumi, FUKUMOTO Sou, USHIMARU Atushi

  Joint 2014 Annual Meeting British Ecological Society and Société Française d’Ecologie, Dec. 2014, English, Lille, France, International conference

  Poster presentation


• 魚類環境DNA用ユニバーサルプライマーの開発と次世代シーケンサを用いた分析法の確立

  MIYA Masaki, SATO Yukuto, FUKUNAGA Tsukasa, SADO Tetsuya, SATO Keiichi, MINAMOTO Toshifumi, YAMANAKA Hiroki, ARAKI Hitoshi, IWASAKI Wataru

  2014年度日本魚類学会年会, Nov. 2014, Japanese, 神奈川県小田原市, Domestic conference

  Oral presentation


• Development of a method for detecting inhabitation of the Dojo loach using environmental DNA

  KOIZUMI Noriyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi, DOI Hideyuki, MORI Atsushi, WATABE Keiji, TAKEMURA Takeshi

  PAWEES 2014 International Conference, Oct. 2014, English, Kaohsiung, Taiwan, International conference

  Oral presentation


• 集団における外来遺伝子頻度を定量する環境DNA手法の開発と野外への適用

  UCHII Kimiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki

  日本陸水学会第79回大会, Sep. 2014, Japanese, 茨城県つくば市, Domestic conference

  Oral presentation


• 環境DNA手法の希少生物種調査への応用: 兵庫県下のため池におけるカワバタモロコの分布調査

  MINAMOTO Toshifumi, FUKUOKA Arisa, TAKAHARA Teruhiko, Biology Club of, Hyogo, Prefectural Agricultural, High School

  日本陸水学会第79回大会, Sep. 2014, Japanese, 茨城県つくば市, Domestic conference

  Oral presentation


• ため池の動物プランクトン群集の規定要因:生産性,生態系サイズ,捕食者に着目した解析

  KATANO Izumi, NKURA Asuko, HAMANO Sayaka, DOI Hideyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi

  日本陸水学会第79回大会, Sep. 2014, Japanese, 茨城県つくば市, Domestic conference

  Poster presentation


• Community ecology using environmental DNA

  DOI Hideyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi

  Evolutionary Community Ecology 2014, Sep. 2014, English, Kyoto, International conference

  [Invited]

  Invited oral presentation


• 水から抽出したDNAを用いて生息魚類を検出する方法の試み

  KOIZUMI Noriyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi, DOI Hideyuki, WATANABE Keiji, MORI Atsushi, TAKEMURA Takeshi

  平成26年度農業農村工学会大会講演会, Aug. 2014, Japanese, 新潟県新潟市, Domestic conference

  Oral presentation


• The Japanese Society for Ecology and Health: J-EcoHealth - An Introduction

  J-Ecohealth

  EcoHealth2014 (The 5th Biennial Conference of the International Association for Ecology & Health), Aug. 2014, English, Montréal, Canada, International conference

  Poster presentation


• Monitoring upstream migration of fish using environmental DNA: Towards a more efficient method for assessing habitat connectivity

  YAMANAKA Hiroki, MINAMOTO Toshifumi

  2014 ESA Annual Meeting, Aug. 2014, English, Sacramento, CA, USA, International conference

  Poster presentation


• Marine fish surveys using environmental DNA

  MINAMOTO Toshifumi, MASUDA Reiji, TAKAHASHI Kohji, MARUYAMA Atsushi, YAMANAKA Hiroki, KASAI Akihide, KONDOH Michio

  2014 ESA Annual Meeting, Aug. 2014, English, Sacramento, CA, USA, International conference

  Poster presentation


• Impact of expansion of wet rice fields on the risk of liver fluke infection in Lao P.D.R.

  MOJI Kazuhiko, PONGVONGSA Tiengkham, PHONGMANY Panom, KOUNNAVONG Sengchanh, AKKAVONG Kongsap, PHROMMALA Souraxay, BOUPHA Boungnong, TOJO Bunpei, JIANG Honwei, NISHIMOTO Futoshi, SATO Megumi, MINAMOTO Toshifumi, KOHMATSU Yukihiro, MARUYAMA Atsushi, FUNATSU Kohei, IWASAKI Shinpei, TOMITA Shinsuke, TOMOKAWA Sachi, KANEDA Eiko, WIKAGUL Jitra, WATANABE Chiho

  EcoHealth2014 (The 5th Biennial Conference of the International Association for Ecology & Health), Aug. 2014, English, Montréal, Canada, International conference

  Poster presentation


• Why probability literacy is important to resolve framing conflicts in environmental issues?

  EBINA Kuniyoshi, IWAMURA Yasunobu, KAWAKATSU Kazuya, OKABE Yasuyuki, UMEMURA Kaito, ODA Toshikatsu, MINAMOTO Toshifumi, NAKAYAMA Akie, ITOH Masayuki

  14th SCA Annual Conferenc, Jun. 2014, English, Kuala Lumpur, Malaysia, International conference

  Oral presentation


• 同種内外来種の侵入規模の迅速把握

  UCHII Kimiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki

  日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference

  [Invited]

  Nominated symposium


• 水生生物の移動分散モニタリングへの環境DNA技術の適用：流水環境における研究例と展望

  YAMANAKA Hiroki, MINAMOTO Toshifumi, SAKURAI Shoh, OHGAKI Suzuka

  日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference

  [Invited]

  Nominated symposium


• 環境DNAとフェノロジー研究: 現状と未来

  DOI Hideyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi

  日本生態学会第61回全国大会, Mar. 2014, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 核DNAをマーカーとした環境DNA解析

  MINAMOTO Toshifumi

  日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference

  [Invited]

  Nominated symposium


• オオクチバス等の外来魚モニタリングにおける環境DNA技術の有用性の検証－調査手法の違いによる結果の比較を通して―

  TAKAHARA Teruhiko, MINAMOTO TOSHIFUMI, DOI Hideyuki, KIZUKA Toshikazu, MITSUO Yoshito, TSUNODA Hiroshi, TAKAMURA Noriko

  日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference

  [Invited]

  Nominated symposium


• Large-scale environmental DNA assessment for Japanese and Chinese giant salamanders in Katsura River, Japan

  FUKUMOTO Sou, USHIMARU Atushi, MINAMOTO Toshifumi

  Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, Mar. 2014, English, Higashi-Hiroshima City, Japan, International conference

  Poster presentation


• Environmental DNA release velocity from different sized fish

  MARUYAMA Atsushi, NAKAMURA Keisuke, YAMANAKA Hiroki, KONDOH Michio, MINAMOTO Toshifumi

  The 61st Annual Meeting of the Ecological Society of Japan, Mar. 2014, English, Hiroshima City, Japan, Domestic conference

  Poster presentation


• 環境DNA分析による生物の分布および生物量の推定技術

  YAMANAKA Hiroki, MINAMOTO Toshifumi

  第25回龍谷大学新春技術講演会, Jan. 2014, Japanese, 滋賀県大津市, Domestic conference

  Poster presentation


• 環境DNA技術で多様性の湖に挑む

  Maruyama Atsushi, KONDOH Michio, YAMANAKA Hiroki, MORIGUCHI Koya, MINAMOTO Toshifumi, RUSUWA Bosco

  第25回龍谷大学新春技術講演会, Jan. 2014, Japanese, 滋賀県大津市, Domestic conference

  Poster presentation


• 見えない生き物を見つけ出す環境DNA技術

  MINAMOTO TOSHIFUMI

  夢・化学21 高校生のための化学講座（浜松）, Dec. 2013, Japanese, 静岡大学（静岡県浜松市）, Domestic conference

  Public discourse


• 環境DNAを用いた水中生物モニタリングの現状

  MINAMOTO TOSHIFUMI

  第29回個体群生態学会大会, Oct. 2013, Japanese, 大阪府堺市, Domestic conference

  [Invited]

  Nominated symposium


• 環境DNAを利用した同種内外来種の迅速把握

  UCHII Kimiko, DOI Hideyuki, MINAMOTO TOSHIFUMI, YAMANAKA Hiroki

  日本陸水学会第78回大会, Sep. 2013, Japanese, 滋賀県大津市, Domestic conference

  Poster presentation


• 環境DNAを用いた在来および外来オオサンショウウオの検出

  FUKUMOTO So, USHIMARU Atushi, MINAMOTO Toshifumi

  日本陸水学会第78回大会, Sep. 2013, Japanese, 滋賀県大津市, Domestic conference

  Oral presentation


• STIに向けた政策プロセスへの関心層別関与フレーム設計

  KUDO Mitsuru, KANO Kei, MIZUMACHI Eri, AKIYA Naonori, MORIMURA Yoshitaka, TAKANASHI Katsuya, MORI Mikihiko, MOTOKI Tamaki, GOTO Takayuki, YOSHIZAWA Go, SUGA Makiko, ITOH Masayuki, EBINA Kuniyoshi, MINAMOTO Toshifumi, NAKAYAMA Akie, MAENAMI Haruhiko, HIOKI Koichiro, DING Xiaojun

  第8回科学コミュニケーション研究会年次大会, Sep. 2013, Japanese, 京都大学, Domestic conference

  Poster presentation


• 上海における環境意識に関する質問票調査

  TAKANO Kohei, JIANG Hong-Wei, FUKUSHI Yuki, CHANG Wen-Ming, MINAMOTO Toshifumi

  中国環境問題研究拠点 国際シンポジウム「東アジアにおける都市化と福祉・環境問題—上海を中心に—」, Jul. 2013, Japanese, 総合地球環境学研究所 中国環境問題研究拠点, 総合地球環境学研究所, International conference

  [Invited]

  Invited oral presentation


• Using environmental DNA to estimate the distributions and biomass of fish

  TAKAHARA Teruhiko, DOI Hideyuki, MINAMOTO Toshifumi

  26th International Congress for Conservation Biology (ICCB 2013), Jul. 2013, English, Baltimore, MD, USA, International conference

  [Invited]

  Nominated symposium


• 川の水をはかれば生き物がわかる？～環境DNA研究の最先端～

  MINAMOTO Toshifumi

  サイエンス・カフェひょうごin佐用, Mar. 2013, Japanese, 兵庫県佐用町, Domestic conference

  Public discourse


• 先端技術（環境DNA，次世代シーケンス）を使って生態学を変えていくには？

  DOI Hideyuki, TAKAHARA Teruhiko, MINAMOTO Toshifumi

  日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference

  [Invited]

  Nominated symposium


• 環境DNA分析の実用化：野外で使える前処理法の検討

  NAKA Takafumi, MINAMOTO Toshifumi, MOJI Kazuhiko, MARUYAMA Atsushi

  日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference

  Poster presentation


• 環境DNA技術を用いた外来種モニタリング手法の開発

  TAKAHARA Teruhiko, DOI Hideyuki, MINAMOTO Toshifumi

  日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference

  Oral presentation


• 環境DNAを用いてタイ肝吸虫の生態を探る

  MINAMOTO Toshifumi, NAKA Takafumi, FUNATSU Kohei, KOHMATSU Yukihiro, KAWABATA Zen'ichiro, MARUYAMA Atsushi, Tiengkham Pongvongsa, MOJI Kazuhiko

  日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference

  Poster presentation


• 環境DNAを用いた魚類の在不在判定による遡河行動のモニタリング

  YAMANAKA Hiroki, MINAMOTO Toshifumi

  日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference

  Oral presentation


• メコン中流域の魚類の移動と寄生虫感染率の変化

  FUNATSU Kohei, MARUYAMA Atsushi, KOHMATSU Yukihiro, MINAMOTO Toshifumi, MOJI Kazuhiko

  日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference

  Poster presentation


• 環境DNAを用いた微生物マッピング：コイヘルペスウイルスを例に

  MINAMOTO Toshifumi

  第二回同位体環境学シンポジウム, Feb. 2013, Japanese, 京都府京都市, Domestic conference

  Nominated symposium


• 環境改変と感染症―琵琶湖のコイヘルペスウイルス病を例に―

  MINAMOTO Toshifumi

  国際シンポジウム「湖の現状と未来可能性」, Jan. 2013, Japanese, 中国上海市, International conference

  Nominated symposium


• Detection and quantification of fish presence and biomass using environmental DNA to monitor population sustainability

  TAKAHARA Teruhiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki, KAWABATA Zen'ichiro

  The 9th International Conference on Environmental, Cultural, Economic and Social Sustainability, Jan. 2013, English, Hiroshima City, Japan, International conference

  Poster presentation


• Monitoring of fish pathogenic viruses in freshwater environments

  MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  EcoHealth2012, Oct. 2012, English, Kunming City, China, International conference

  Poster presentation


• 環境水中のウィルス定量：水域生態系での感染症研究への適用

  HONJO Mie N, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 名古屋大学, Domestic conference

  [Invited]

  Invited oral presentation


• 環境DNAを用いた魚類相の定性的把握法

  MINAMOTO Toshifumi, YAMANAKA Hiroki, TAKAHARA Teruhiko, HONJO Mie N, KAWABATA Zen'ichiro

  日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 愛知県名古屋市, Domestic conference

  [Invited]

  Invited oral presentation


• 核酸の動態から感染症の生態を探る

  UCHII Kimiko, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 名古屋大学, Domestic conference

  [Invited]

  Invited oral presentation


• ため池の水生動物モニタリングに環境DNAを応用する

  TAKAHARA Teruhiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki, KAWABATA Zen'ichiro

  日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 愛知県名古屋市, Domestic conference

  [Invited]

  Invited oral presentation


• Transmission dynamics of an emerging pathogen Cyprinid herpesvirus 3 and its impact on host population genetic structure

  UCHII Kimiko, OKUDA Noboru, MINAMOTO Toshifumi, TELSHOW Arndt, KAWABATA Zen'ichiro

  2012 ASLO Aquatic Sciences Meeting, Jul. 2012, English, ASLO, Otsu City, Shiga, Japan, International conference

  Oral presentation


• Seasonal and spatial distribution of Cyprinid herpesvirus 3 in water and sediment of a lagoon of Lake Biwa, Japan

  HONJO Mie N, MINAMOTO Toshifumi, YAMANAKA Hiroki, TAKAHARA Teruhiko, KAWABATA Zen'ichiro

  2012 ASLO Aquatic Sciences Meeting, Jul. 2012, English, ASLO, Otsu City, Shiga, Japan, International conference

  Poster presentation


• Detection and quantification of fish presence/biomass in ponds using environmental DNA

  TAKAHARA Teruhiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki, KAWABATA Zen'ichiro

  2012 ASLO Aquatic Sciences Meeting, Jul. 2012, English, ASLO, Otsu City, Shiga, Japan, International conference

  Poster presentation


• 新興病原体コイヘルペスウイルスの野生宿主個体群における持続性

  UCHII Kimiko, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  日本生態学会第59回大会／第５回東アジア生態学会連合大会合同大会, Mar. 2012, Japanese, 日本生態学会, EAFES, 滋賀県大津市, Domestic conference

  Oral presentation


• 湖水中に溶存するDNA断片から魚類のバイオマスを推定する

  TAKAHARA Teruhiko, DOI Hideyuki, MINAMOTO Toshifumi, YAMANAKA Hiroki, KAWABATA Zen'ichiro

  日本生態学会第59回大会／第５回東アジア生態学会連合大会合同大会, Mar. 2012, Japanese, 日本生態学会, EAFES, 滋賀県大津市, Domestic conference

  Oral presentation


• Seasonal and spatial distribution of Cyprinid herpesvirus 1 and Cyprinid herpesvirus 2 in Lake Biwa, Japan

  HONJO Mie N, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  Joint Meeting of The 59th Annual Meeting of ESJ and The 5th EAFES International Congress, Mar. 2012, English, 日本生態学会, EAFES, Otsu City, Shiga, Japan, International conference

  Oral presentation


• Dynamics of koi herpesvirus and related factors in freshwater environments (EAFES Symposium ES09: Interactions of pathogen-host with environments)

  MINAMOTO Toshifumi

  Joint Meeting of The 59th Annual Meeting of ESJ and The 5th EAFES International Congress, Mar. 2012, English, Otsu Ciyu, Shiga, Japan, International conference

  Nominated symposium


• 感染症の生態学的解析：コイヘルペスウイルス病を例に

  MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  感染症若手フォーラム2012, Feb. 2012, Japanese, 長崎県長崎市, Domestic conference

  Oral presentation


• 環境の変化と魚の病気

  MINAMOTO Toshifumi

  第10回 地球研地域連携セミナー「水辺の保全と琵琶湖の未来可能性」, Jan. 2012, Japanese, 滋賀県大津市, Domestic conference

  Public discourse


• Koi herpesvirus disease as a model of environmental disease

  MINAMOTO Toshifumi, Members of RIHN, Prohect

  The 6th RIHN International Symposium, Oct. 2011, English, Kyoto City, Japan, International conference

  Nominated symposium


• KHV dynamics in natural freshwater environments.

  MINAMOTO Toshifumi, HONJO Mie N, YAMANAKA Hiroki, UCHII Kimiko, KAWABATA Zen'ichiro

  Emergence of Viral Diseases: Ecology and Evolution of Koi Herpes Virus, Jul. 2011, English, Muenster, Germany, International conference

  Nominated symposium


• Dynamics of Cyprinid herpesvirus 3 in natural environments in Japan

  MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  4th Congress of European Microbiologists, FEMS 2011, Jun. 2011, English, FEMS, Geneva City, Switzerland, International conference

  Poster presentation


• 水温の変動パタンが魚類の生理コストに与える影響について

  YAMANAKA Hiroki, MINAMOTO Toshifumi, TAKAHARA Teruhiko, KAWABATA Zen'ichiro

  日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conference

  Poster presentation


• 環境DNAを用いた魚類相把握法の開発

  MINAMOTO Toshifumi, HONJO Mie N, YAMANAKA Hiroki, KAWABATA Zen'ichiro

  日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conference

  Oral presentation


• コイヘルペスウイルス（KHV）病の発症率はストレスの影響を受けるか？

  TAKAHARA Teruhiko, HONJO Mie N, MINAMOTO Toshifumi, ITOH Takafumi, KAWABATA Zen'ichiro

  日本応用動物昆虫学会第55回大会, Mar. 2011, Japanese, 日本応用動物昆虫学会, 福岡県福岡市, Domestic conference

  Oral presentation


• コイヘルペスウイルス病が琵琶湖の野生型コイへもたらした影響

  UCHII Kimiko, OKUDA Noboru, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conference

  Oral presentation


• コイにとっての岸辺環境の有用性とストレス回避のトレードオフ

  TAKAHARA Teruhiko, YAMANAKA Hiroki, SUZUKI Alata A, HONJO Mie N, MINAMOTO Toshifumi, YONEKURA Ryuji, ITAYAMA Tomoaki, KOHMATSU Yukihiro, ITOH Takafumi, KAWABATA Zen'ichiro

  日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conference

  Poster presentation


• 中国雲单省Erhai湖における水温の時空間分布パターン

  YAMANAKA Hiroki, MINAMOTO Toshifumi, Deyi Wu, Hainan Kong, Zhi-hong Wei, Liu Bin, KAWABATA Zen'ichiro

  日本陸水学会第75回大会, Sep. 2010, Japanese, 日本陸水学会, 青森県弘前市, Domestic conference

  Poster presentation


• 全国の自然河川におけるコイヘルペスウイルスの分布

  MINAMOTO Toshifumi, HONJO Mie N, YAMANAKA Hiroki, UCHII Kimiko, KAWABATA Zen'ichiro

  日本陸水学会第75回大会, Sep. 2010, Japanese, 日本陸水学会, 青森県弘前市, Domestic conference

  Oral presentation


• 魚類の多様性と視物質の分子進化

  MINAMOTO Toshifumi

  平成22年度 日本動物学会中部地区大会公開シンポジウム, Jul. 2010, Japanese, 日本動物学会中部支部会, 岐阜県岐阜市, Domestic conference

  Public discourse


• Seasonal dynamics of CyHV-3 in natural freshwater environments

  MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  Workshop on the Linkage between CyHV-3 (KHV) and Humans, May 2010, English, Jerusalem, Israel, International conference

  Oral presentation


• Detection of cyprinid herpesvirus-3 (CyHV-3) in environmental water and sediments

  HONJO Mie N, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  Workshop on the Linkage between CyHV-3 (KHV) and Humans, May 2010, English, Jerusalem, Israel, International conference

  Oral presentation


• 堆積物におけるコイヘルペスウイルスの検出・定量

  HONJO Mie N, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  日本生態学会第57回大会, Mar. 2010, Japanese, 日本生態学会, 東京都目黒区, Domestic conference

  Oral presentation


• 環境水中の低密度ウイルスに対する濃縮システムの開発

  Tanaka Nobuyuki, ITAYAMA Tomoaki, MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  第44回日本水環境学会年会, Mar. 2010, Japanese, 日本水環境学会, 福岡県福岡市, Domestic conference

  Oral presentation


• コイヘルペスウイルス感染症と人間の相互作用環

  MINAMOTO Toshifumi, HONJO Mie N, UCHII Kimiko, YAMANAKA Hiroki, SUZUKI Alata A, KOHMATSU Yukihiro, YONEKURA Ryuji, OMORI Koji, ITAYAMA Tomoaki, Tanaka Nobuyuki, ASANO Kota, SHIRAE Yusuke, OKUDA Noboru, KAWABATA Zen'ichiro

  日本生態学会第57回大会, Mar. 2010, English, 日本生態学会, 東京都目黒区, Domestic conference

  Nominated symposium


• コイの行動性体温調節と環境水温の時空間的不均一性がコイヘルペスウイルス症の蔓延に与える影響について

  YAMANAKA Hiroki, SOGABE Atsushi, OMORI Koji, MINAMOTO Toshifumi, MIKI Takeshi, SAITO Yasuhisa, UCHII Kimiko, HONJO Mie N, SUZUKI Alata A, KOHMATSU Yukihiro, KAWABATA Zen'ichiro

  日本生態学会第57回大会, Mar. 2010, Japanese, 日本生態学会, 東京都目黒区, Domestic conference

  Oral presentation


• 野外におけるコイの行動性体温調節とその季節変化：コイヘルペスウイルス病蔓延時期との対応について

  YAMANAKA Hiroki, SOGABE Atsushi, OMORI Koji, MINAMOTO Toshifumi, UCHII Kimiko, HONJO Mie N, SUZUKI Alata A, KOHMATSU Yukihiro, KAWABATA Zen'ichiro

  日本陸水学会第74回大会, Sep. 2009, Japanese, 日本陸水学会, 大分県大分市, Domestic conference

  Oral presentation


• 琵琶湖におけるコイヘルペスウイルスの動態解析

  MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  日本陸水学会第74回大会, Sep. 2009, Japanese, 日本陸水学会, 大分県大分市, Domestic conference

  Oral presentation


• Microarray analysis of circadian gene expressions in Ciona intestinalis

  MATSUMAE Hiromi, MINAMOTO Toshifumi, HANAI Shuji, ISHIWATA Ryosuke, Izumi, K, OGISHIMA Soichi, TANAKA Hiroshi, SATOH Nori, ISHIDA Norio

  The 5th International Tunicate Meeting, Jun. 2009, English, Naha City, Okinawa, Japan, International conference

  Poster presentation


• Seasonal distribution of cyprinid herpesvirus 3 in Lake Biwa

  MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  Workshop on CyHV-3 disease in an environment-human linkage, Apr. 2009, English, Kyoto City, Japan, International conference

  Oral presentation


• 琵琶湖におけるコイヘルペスウイルスの分布と季節変化

  MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  日本生態学会第56回大会, Mar. 2009, Japanese, 日本生態学会, 岩手県盛岡市, Domestic conference

  Oral presentation


• 湖岸形態と水温分布：魚類への影響の考察

  YAMANAKA Hiroki, KOHMATSU Yukihiro, MINAMOTO Toshifumi, HONJO Mie N, UCHII Kimiko, SUZUKI Alata A, KAWABATA Zen'ichiro

  日本生態学会第56回大会, Mar. 2009, Japanese, 日本生態学会, 岩手県盛岡市, Domestic conference

  Poster presentation


• ウイルス状粒子の迅速濃縮法とKHVの検出

  Tanaka Nobuyuki, ITAYAMA Tomoaki, MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  第42回日本水環境学会大会, Mar. 2009, Japanese, 日本水環境学会, 愛知県名古屋市, Domestic conference

  Oral presentation


• カタユウレイボヤの概日リズム

  MATSUMAE Hiromi, MINAMOTO Toshifumi, HANAI Shuji, OISHI Katsutaka, AZUMI Kaoru, ISHIWATA Ryusuke, OGISHIMA Soichi, TANAKA Hiroshi, SATOH Nori, ISHIDA Norio

  第15回日本時間生物学会学術大会, Nov. 2008, Japanese, 日本時間生物学会, 岡山県岡山市, Domestic conference

  Poster presentation


• 環境水中に存在するコイヘルペスウイルスの定量

  HONJO Mie N, MINAMOTO Toshifumi, MATSUI Kazuaki, UCHII Kimiko, YAMANAKA Hiroki, SUZUKI Alata A, KOHMATSU Yukihiro, IIDA Takaji, KAWABATA Zen'ichiro

  日本陸水学会第73回大会, Oct. 2008, Japanese, 日本陸水学会, 北海道札幌市, Domestic conference

  Oral presentation


• Development of a Rapid Concentration System for Virus in Environmental Water

  TANAKA Nobuyuki, ITAYAMA Tomoaki, HONJO Mie N, MINAMOTO Toshifumi, KAWABATA Zen'ichiro

  12th International Conference on Integrated Diffuse Pollution Management (IWA DIPCON 2008), Aug. 2008, English, Khon Kaen City, Thailand, International conference

  Oral presentation


• Relationship of lake morphometry and shore configuration to the temperature distribution in lagoons, and implications for its effect on fish health

  YAMANAKA Hiroki, SOGABE Atsushi, KOHMATSU Yukihiro, MINAMOTO Toshifumi, HONJO Mie. N, UCHII Kimiko, SUZUKI Alata A, OMORI Koji, KAWABATA Zen'ichiro

  International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conference

  Poster presentation


• Quantification method of Koi herpesvirus (KHV) in environmental water using cation-coated filter method and external standard virus

  HONJO Mie N, MINAMOTO Toshifumi, MATSUI Kazuaki, UCHII Kimiko, YAMANAKA Hiroki, SUZUKI Alata A, KOHMATSU Yukihiro, KAWABATA Zen'ichiro

  International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conference

  Poster presentation


• International Symposium on Environmental Change, Pathogens, and Human Linkages

  KAWABATA Zen'ichiro, MINAMOTO Toshifumi, HONJO Mie N, UCHII Kimiko, YAMANAKA Hiroki, SUZUKI Alata A, KOHMATSU Yukihiro

  International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City, Japan, International conference

  Oral presentation


• Development of an on site rapid concentration system for virus in environmental water

  ITAYAMA Tomoaki, TANAKA Nobuyuki, MINAMOTO Toshifumi, HONJO Mie N, KAWABATA Zen'ichiro

  International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conference

  Poster presentation


• Detection of koi herpesvirus DNA from natural river water

  MINAMOTO Toshifumi, HONJO Mie N, UCHII Kimiko, YAMANAKA Hiroki, SUZUKI Alata A, KOHMATSU Yukihiro, KAWABATA Zen'ichiro

  International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conference

  Poster presentation


• カタユウレイボヤのcDNAマイクロアレイの数理解析による時計候補遺伝子の抽出

  MATSUMAE Hiromi, MINAMOTO Toshifumi, HANAI Shuji, OISHI Katsutaka, AZUMI Kaoru, ISHIWATA Ryusuke, OGISHIMA Soichi, TANAKA Hiroshi, SATOH Nori, ISHIDA Norio

  第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会, Dec. 2007, Japanese, 日本分子生物学会, 日本生化学会, 神奈川県横浜市, Domestic conference

  Poster presentation


• カタユウレイボヤ（Ciona intestinalis）の概日リズム

  MINAMOTO Toshifumi, HANAI Shuji, OISHI Katsutaka, KADOTA Koji, SATOH Nori, 佐竹正延, AZUMI Kaoru, ISHIDA Norio

  第13回日本時間生物学会学術大会, Nov. 2006, Japanese, 日本時間生物学会, 東京都千代田区, Domestic conference

  Poster presentation


• カタユウレイボヤ（Ciona intestinalis）における概日振動遺伝子群の探索

  MINAMOTO Toshifumi, HANAI Shuji, OISHI Katsutaka, KADOTA Koji, SATOH Nori, SATAKE Masanobu, AZUMI Kaoru, ISHIDA Norio

  日本動物学会第77回大会, Sep. 2006, Japanese, 日本動物学会, 島根県松江市, Domestic conference

  Oral presentation


• カタユウレイボヤ（Ciona intestinalis）の概日振動遺伝子群

  MINAMOTO Toshifumi, HANAI Shuji, OISHI Katsutaka, 門田幸二, SATOH Nori, 佐竹正延, AZUMI Kaoru, ISHIDA Norio

  第12回日本時間生物学会大会, Nov. 2005, Japanese, 日本時間生物学会, 茨城県つくば市, Domestic conference

  Poster presentation


• ニホンミツバチにおける新たなperiod遺伝子 mRNAバリアントの解析

  MINAMOTO Toshifumi, FUCHIKAWA Taro, SHIMIZU Yoshinori, SHIMIZU Isamu

  日本動物学会第75回大会, Sep. 2004, Japanese, 日本動物学会, 神戸市灘区, Domestic conference

  Poster presentation


• ニホンミツバチ及び膜翅目昆虫におけるperiod遺伝子mRNAバリアントの解析

  MINAMOTO Toshifumi, SHIMIZU Isamu

  日本動物学会第74回大会, Sep. 2003, Japanese, 日本動物学会, 北海道函館市, Domestic conference

  Oral presentation


• アユの錐体視物質遺伝子に関する研究

  MINAMOTO Toshifumi, SHIMIZU Isamu

  日本動物学会第73回大会, Sep. 2002, Japanese, 日本動物学会, 石川県金沢市, Domestic conference

  Oral presentation


• Studies of opsin genes in a smelt fish, Ayu (Plecoglossus altivelis).

  MINAMOTO Toshifumi, SHIMIZU Isamu

  The 1st Asian Conference on Photobiology, Jun. 2002, English, Higashiura Town, Hyogo, Japan, International conference

  Poster presentation


• アユのVAオプシンに関する研究

  MINAMOTO Toshifumi, SHIMIZU Isamu

  日本動物学会第72回大会, Oct. 2001, Japanese, 日本動物学会, 福岡県福岡市, Domestic conference

  Oral presentation


• バイカル湖における水中光の分光放射特性

  MINAMOTO Toshifumi, SHIMIZU Isamu, MICHINOMAE Masanao, HIGASHI Masahiko, NAKANISHI Masami

  第65回日本陸水学会大会, Sep. 2000, Japanese, 日本陸水学会, 福岡県福岡市, Domestic conference

  Oral presentation


• アユの視物質遺伝子に関する研究（２）

  MINAMOTO Toshifumi, SHIMIZU Isamu

  日本動物学会第70回大会, Sep. 1999, Japanese, 日本動物学会, 山形県山形市, Domestic conference

  Oral presentation


• 魚類の視物質及びチトクロームb遺伝子の分子進化

  MINAMOTO Toshifumi, SHIMIZU Isamu

  日本動物学会第68回大会, Oct. 1997, Japanese, 日本動物学会, 奈良県奈良市, Domestic conference

  Oral presentation