Research activity information

• Mar. 2016 日本農芸化学会, BBB Most Cited Paper Award, Efficient Agrobacterium-mediated transformation of the liverwort Marchantia polymorpha using regenerating thalli. Bioscience, Biotechnology, and Biochemistry Vol.77, No.1, pp.167～172

  久保田 茜, ISHIZAKI KIMITSUNE, 保坂 将志, 河内 孝之

  論文発表後2年間で最も引用回数が多かった。

  Official journal


• Mar. 2015 The Japanese Society of Plant Physiologists, The Japanese Society of Plant Physiologists, 基部陸上植物ゼニゴケにおける器官発生制御機構の解析

  ISHIZAKI KIMITSUNE

  International society


• Mar. 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry, BBB論文賞, Efficient Agrobacterium-Mediated Transformation of the Liverwort Marchantia polymorpha Using Regenerating Thalli., Bioscience, Biotechnology, and Biochemistry Vol. 77 (2013) No. 1 p. 16

  Akane Kubota, ISHIZAKI KIMITSUNE, Masashi Hosaka, Takayuki Kohchi

  Official journal


• Sep. 2013 日本植物学会, JPR論文賞, Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha.

  ISHIZAKI KIMITSUNE, Maiko Nonomura, Hirotaka Kato, Katsuyuki T. Yamato, Takayuki Kohchi

  Ishizaki et al. (2012)は、陸上植物の発生過程で重要なはたらきをするオーキシンによる分子制御機構が、すでに非維管束植物であるゼニゴケで始まっていたことを示す論文です。モデル植物であるシロイヌナズナで得られた知見をいかして、植物生理学の重要課題に進化の視点から切り込んだ優れた論文です。

  Official journal


• Apr. 2013 Japan Society for Bioscience, Biotechnology, and Agrochemistry, JSBBA Award for Young Scientists, 光合成生物における生存戦略の分子機構に関する研究

  ISHIZAKI KIMITSUNE

  Japan society

• Divergent evolution of a thermospermine-dependent regulatory pathway in land plants.

  Anna Solé-Gil, Yuuki Sakai, Bruno Catarino, Victor A S Jones, Christopher E Youngstrom, Joan Jordà-Segura, Chi-Lien Cheng, Liam Dolan, Barbara A Ambrose, Kimitsune Ishizaki, Miguel A Blázquez, Javier Agustí

  Plants adapted to life on land by developing diverse anatomical features across lineages. The molecular basis of these innovations often involves the emergence of new genes or establishing new connections between conserved elements, though evidence for evolutionary genetic circuit rewiring remains scarce. Here, we show that the thermospermine-dependent pathway regulating vascular cell proliferation in Arabidopsis thaliana operates as two distinct modules with different functions in the bryophyte Marchantia polymorpha. One module controls dichotomous branching at meristems, while the other one modulates gemmae and rhizoid production in the thallus. Heterologous assays and comparative expression analyses reveal that the molecular links between these modules, forming a unified circuit in vascular plants, emerged early in tracheophyte evolution. Our results illustrate how the thermospermine-dependent circuit elements followed two divergent evolutionary trajectories in bryophytes and tracheophytes, eventually influencing distinct developmental processes.

  Dec. 2024, Developmental Cell, English, International magazine

  [Refereed]

  Scientific journal


• Evolutionary conserved RLF, a plant cytochromeb₅-like heme-binding protein, is essential for organ development inMarchantia polymorpha

  Kentaro P. Iwata, Takayuki Shimizu, Yuuki Sakai, Tomoyuki Furuya, Hinatamaru Fukumura, Yuki Kondo, Tatsuru Masuda, Kimitsune Ishizaki, Hidehiro Fukaki

  InArabidopsis thaliana, REDUCED LATERAL ROOT FORMATION (RLF), a cytochromeb₅-like heme-binding domain (Cytb5-HBD) protein, is necessary for proper lateral root formation. Whereas the other Cytb5-HBD proteins inA. thalianaregulate different metabolic reactions, RLF is unique as it specifically regulates organ development. However, it remains unknown whether heme binding to RLF is necessary for its function, and whetherRLForthologs in different plant species also regulate organ development. We demonstrate that RLF binds to hemein vitroand that the two histidine residues, which are conserved among Cytb5-HBD, are crucial for both heme binding and its biological function inA. thaliana. In addition, MpRLF, aRLFortholog inMarchantia polymorpha, rescues the lateral root formation phenotype of theA. thaliana rlfmutant. Mprlf^ge, the loss-of-function mutation in the MpRLF, resulted in delayed thallus growth and inhibited both gemma cup and antheridiophore formation. Transcriptome analysis using Mprlf^gerevealed that MpRLFaffects several metabolic pathways. Our findings indicate that MpRLFis essential for vegetative and reproductive development inM. polymorpha, suggesting that the RLF-dependent redox reaction systems are evolutionarily conserved as crucial mechanisms for organ development across diverse plant species.

  Cold Spring Harbor Laboratory, Sep. 2024


• A kinesin-like protein, KAC, is required for light-induced and actin-based chloroplast movement in Marchantia polymorpha.

  Yoshiko Yamamoto-Negi, Takeshi Higa, Aino Komatsu, Kanta Sasaki, Kimitsune Ishizaki, Ryuichi Nishihama, Eiji Gotoh, Takayuki Kohchi, Noriyuki Suetsugu

  Chloroplasts accumulate on the cell surface under weak light conditions to efficiently capture light but avoid strong light to minimize photodamage. The blue light receptor phototropin regulates the chloroplast movement in various plant species. In Arabidopsis thaliana, phototropin mediates the light-induced chloroplast movement and positioning via specialized actin filaments on the chloroplasts, chloroplast-actin filaments. KINESIN-LIKE PROTEIN FOR ACTIN-BASED CHLOROPLAST MOVEMENT (KAC) and CHLOROPLAST UNUSUAL POSITIONING 1 (CHUP1) are pivotal for chloroplast-actin-based chloroplast movement and positioning in land plants. However, the mechanisms by which KAC and CHUP1 regulate chloroplast movement and positioning remain unclear. In this study, we characterized KAC and CHUP1 orthologs in the liverwort Marchantia polymorpha, MpKAC and MpCHUP1, respectively. Their knockout mutants, Mpkack° and Mpchup1k°, impaired the light-induced chloroplast movement. Although Mpchup1k° showed mild chloroplast aggregation, Mpkack° displayed severe chloroplast aggregation, suggesting the greater contribution of MpKAC to the chloroplast anchorage to the plasma membrane. Analysis of the subcellular localization of the functional MpKAC-Citrine indicated that MpKAC-Citrine formed a punctate structure on the plasma membrane. Structure-function analysis of MpKAC revealed that a deletion of the conserved C-terminal domain abrogates the targeting to the plasma membrane and its function. A deletion of the N-terminal motor domain retained the plasma membrane targeting but abrogates the formation of punctate structure and showed severe defect in the light-induced chloroplast movement. Our findings suggest that the formation of the punctate structure on the plasma membrane of MpKAC is essential for chloroplast movement.

  Aug. 2024, Plant & Cell Physiology, 65(11) (11), 1787 - 1800, English, Domestic magazine

  [Refereed]

  Scientific journal


• SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions

  Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima

  Abstract Land plants have evolved many systems to adapt to a wide range of environmental stresses. In seed plants, oligogalactolipid synthesis is involved in tolerance to freezing and dehydration, but it has not been analyzed in non-vascular plants. Here we analyzed trigalactosyldiacylglycerol (TGDG) synthesis in Marchantia polymorpha. TGDG is synthesized by galactolipid: galactolipid galactosyltransferase [GGGT; SENSITIVE TO FREEZING2 (SFR2) in Arabidopsis]. We analyzed the subcellular localization and GGGT activity of two M. polymorpha SFR2 homologs (MpGGGT1 and MpGGGT2, each as a GFP-fusion protein) using a transient expression system in Nicotiana benthamiana leaves and found that MpGGGT1-GFP localized in the chloroplast envelope membrane. We produced mutants Mpgggt1 and Mpgggt2 and found that TGDG did not accumulate in Mpgggt1 upon treatment of the thallus with acetic acid. Moreover, growth of Mpgggt1 mutants was impaired by acetic acid treatment. Microscopy revealed that the acetic acid treatment of M. polymorpha plants damaged intracellular membranes. The fact that the effect was similar for wild-type and Mpgggt1 plants suggested that MpGGGT has a role in recovery from damage. These results indicate that MpGGGT plays a crucial role in M. polymorpha growth under conditions of acid stress, which may have been encountered during the ancient terrestrial colonization of plants.

  Springer Science and Business Media LLC, Aug. 2024, Journal of Plant Research, 137, 1115 - 1126, English

  [Refereed]

  Scientific journal


• Integration of cell differentiation and initiation of monoterpenoid indole alkaloid metabolism in seed germination of Catharanthus roseus.

  Mai Uzaki, Tetsuya Mori, Mayuko Sato, Mayumi Wakazaki, Noriko Takeda-Kamiya, Kotaro Yamamoto, Akio Murakami, Delia Ayled Serna Guerrero, Chizuko Shichijo, Miwa Ohnishi, Kimitsune Ishizaki, Hidehiro Fukaki, Sarah E O'Connor, Kiminori Toyooka, Tetsuro Mimura, Masami Yokota Hirai

  In Catharanthus roseus, monoterpenoid indole alkaloids (MIAs) are produced through the cooperation of four cell types, with final products accumulating in specialized cells known as idioblasts and laticifers. To explore the relationship between cellular differentiation and cell type-specific MIA metabolism, we analyzed the expression of MIA biosynthesis in germinating seeds. Embryos from immature and mature seeds were observed via stereomicroscopy, fluorescence microscopy, and electron microscopy. Time-series MIA and iridoid quantification, along with transcriptome analysis, were conducted to determine the initiation of MIA biosynthesis. In addition, the localization of MIAs was examined using alkaloid staining and imaging mass spectrometry (IMS). Laticifers were present in embryos before seed maturation. MIA biosynthesis commenced 12 h after germination. MIAs accumulated in laticifers of embryos following seed germination, and MIA metabolism is induced after germination in a tissue-specific manner. These findings suggest that cellular morphological differentiation precedes metabolic differentiation. Considering the well-known toxicity and defense role of MIAs in matured plants, MIAs may be an important defense strategy already in the delicate developmental phase of seed germination, and biosynthesis and accumulation of MIAs may require the tissue and cellular differentiation.

  May 2024, New Phytologist, 242(3) (3), 1156 - 1171, English, International magazine

  [Refereed]

  Scientific journal


• Non-canonical BZR/BES transcription factor regulates the developmental process of haploid reproductive organs in the liverwort Marchantia polymorpha

  Tomoyuki Furuya, Natsumi Saegusa, Shohei Yamaoka, Yuki Tomoita, Naoki Minamino, Masaki Niwa, Keisuke Inoue, Chiaki Yamamoto, Kazuki Motomura, Shunji Shimadzu, Ryuichi Nishihama, Kimitsune Ishizaki, Takashi Ueda, Hidehiro Fukaki, Takayuki Kohchi, Hiroo Fukuda, Masahiro Kasahara, Takashi Araki, Yuki Kondo

  Apr. 2024, Nature Plants, 10(未定) (未定), 未定 - 未定, English

  [Refereed]

  Scientific journal


• Regulation of ROP GTPase cycling between active/inactive states is essential for vegetative organogenesis inMarchantia polymorpha

  Yuuki Sakai, Aki Ueno, Hiroki Yonetsuka, Tatsuaki Goh, Hirotaka Kato, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

  Rho/Rac of plant (ROP) GTPases are a plant-specific subfamily of Rho small GTP-binding proteins that function as molecular switches by being converted to the active state by guanine nucleotide exchange factors (GEFs) and to the inactive state by GTPase-activating proteins (GAPs). The bryophyteMarchantia polymorphacontains single-copy genes encoding ROP (MpROP), two types of GEFs (ROPGEF and SPIKE (SPK)), and two types of GAPs (ROPGAP and ROP enhancer (REN)). MpROP regulates the development of various organs, including the air chambers, rhizoids, and clonal propagule gemmae. While the sole PRONE-type ROPGEF, KARAPPO (MpKAR), plays an essential role in gemma initiation, little is known about thein-plantafunctions of other ROP regulatory factors inM. polymorpha. In this study, we focused on the functions of two types of GAPs: MpROPGAP and MpREN. Loss-of-function Mpren^gesingle mutants showed pleiotropic defects in thallus growth, air chamber formation, rhizoid tip growth, and gemma development, whereas MpROPGAP mutants showed no detectable abnormalities. Despite the distinctive domain structures of MpROPGAP and MpREN, Mpropgap^geMpren^gedouble mutants showed more severe phenotypes than the Mpren^gesingle mutants, suggesting redundant functions of MpROPGAP and MpREN in gametophyte organogenesis. Interestingly, overexpression of MpROPGAP, MpREN, anddominant-negativeMpROP(MpROP^DN) resulted in similar air chamber defects, as well as loss-of-function of MpRENand MpROPGAPand overexpression ofconstitutively activeMpROP(MpROP^CA), suggesting importance of activation/inactivation cycling (or balancing) of MpROP. Furthermore, we proved the contributions of the sole DOCK family GEF, MpSPK, to MpROP-regulated air chamber formation. In summary, our results demonstrate a significant role of the two GAPs in the development of various organs and that the two GEFs are responsible for organogenesis through the control of the MpROP active/inactive cycle in the vegetative growth ofM. polymorpha.

  Cold Spring Harbor Laboratory, Apr. 2024


• Phosphatidic acid phosphohydrolase modulates glycerolipid synthesis in Marchantia polymorpha and is crucial for growth under both nutrient-replete and -deficient conditions

  Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima

  Abstract Main conclusion The phosphatidic acid phosphohydrolase of Marchantia polymorpha modulates plastid glycolipid synthesis through the ER pathway and is essential for normal plant development regardless of nutrient availability. Abstract Membrane lipid remodeling is one of the strategies plant cells use to secure inorganic phosphate (Pi) for plant growth, but many aspects of the molecular mechanism and its regulation remain unclear. Here we analyzed membrane lipid remodeling using a non-vascular plant, Marchantia polymorpha. The lipid composition and fatty acid profile during Pi starvation in M. polymorpha revealed a decrease in phospholipids and an increase in both galactolipids and betaine lipids. In Arabidopsis thaliana, phosphatidic acid phosphohydrolase (PAH) is involved in phospholipid degradation and is crucial for tolerance to both Pi and nitrogen starvation. We produced two M. polymorpha PAH (MpPAH) knockout mutants (Mppah-1 and Mppah-2) and found that, unlike Arabidopsis mutants, Mppah impaired plant growth with shorter rhizoids compared with wild-type plants even under nutrient-replete conditions. Mutation of MpPAH did not significantly affect the mole percent of each glycerolipid among total membrane glycerolipids from whole plants under both Pi-replete and Pi-deficient conditions. However, the fatty acid composition of monogalactosyldiacylglycerol indicated that the amount of plastid glycolipids produced through the endoplasmic reticulum pathway was suppressed in Mppah mutants. Phospholipids accumulated in the mutants under N starvation. These results reveal that MpPAH modulates plastid glycolipid synthesis through the endoplasmic reticulum pathway more so than what has been observed for Arabidopsis PAH; moreover, unlike Arabidopsis, MpPAH is crucial for M. polymorpha growth regardless of nutrient availability.

  Springer Science and Business Media LLC, Oct. 2023, Planta, 258(5) (5), 92, English

  [Refereed]

  Scientific journal


• Genetic Interaction between Arabidopsis SUR2/CYP83B1 and GNOM Indicates the Importance of Stabilizing Local Auxin Accumulation in Lateral Root Initiation

  Chieko Goto, Akira Ikegami, Tatsuaki Goh, Kaisei Maruyama, Hiroyuki Kasahara, Yumiko Takebayashi, Yuji Kamiya, Koichi Toyokura, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki

  Abstract Lateral root (LR) formation is an important developmental event for the establishment of the root system in most vascular plants. In Arabidopsis thaliana, the fewer roots (fwr) mutation in the GNOM gene, encoding a guanine nucleotide exchange factor of ADP ribosylation factor that regulates vesicle trafficking, severely inhibits LR formation. Local accumulation of auxin response for LR initiation is severely affected in fwr. To better understand how local accumulation of auxin response for LR initiation is regulated, we identified a mutation, fewer roots suppressor1 (fsp1), that partially restores LR formation in fwr. The gene responsible for fsp1 was identified as SUPERROOT2 (SUR2), encoding CYP83B1 that positions at the metabolic branch point in the biosynthesis of auxin/indole-3-acetic acid (IAA) and indole glucosinolate. The fsp1 mutation increases both endogenous IAA levels and the number of the sites where auxin response locally accumulates prior to LR formation in fwr. SUR2 is expressed in the pericycle of the differentiation zone and in the apical meristem in roots. Time-lapse imaging of the auxin response revealed that local accumulation of auxin response is more stable in fsp1. These results suggest that SUR2/CYP83B1 affects LR founder cell formation at the xylem pole pericycle cells where auxin accumulates. Analysis of the genetic interaction between SUR2 and GNOM indicates the importance of stabilization of local auxin accumulation sites for LR initiation.

  Oxford University Press (OUP), Jul. 2023, Plant & Cell Physiology, 64(10) (10), 1178 - 1188, English

  [Refereed]

  Scientific journal


• MpDWF5A-Encoded Sterol Δ7-Reductase Is Essential for the Normal Growth and Development of Marchantia polymorpha

  Miki Hatada, Ryota Akiyama, Moeko Yamagishi, Kimitsune Ishizaki, Masaharu Mizutani

  Abstract Sterols are essential components of eukaryotic cell membranes. However, studies on sterol biosynthesis in bryophytes are limited. This study analyzed the sterol profiles in the bryophyte model plant Marchantia polymorpha L. The thalli contained typical phytosterols such as campesterol, sitosterol and stigmasterol. BLASTX analysis of the M. polymorpha genome against the Arabidopsis thaliana sterol biosynthetic genes confirmed the presence of all the enzymes responsible for sterol biosynthesis in M. polymorpha. We further focused on characterizing two genes, MpDWF5A and MpDWF5B, which showed high homology with A. thaliana DWF5, encoding Δ5,7-sterol Δ7-reductase (C7R). Functional analysis using a yeast expression system revealed that MpDWF5A converted 7-dehydrocholesterol to cholesterol, indicating that MpDWF5A is a C7R. Mpdwf5a-knockout (Mpdwf5a-ko) lines were constructed using CRISPR/Cas9-mediated genome editing. Gas chromatography–mass spectrometry analysis of Mpdwf5a-ko revealed that phytosterols such as campesterol, sitosterol and stigmasterol disappeared, and instead, the corresponding Δ7-type sterols accumulated. The thalli of Mpdwf5a-ko grew smaller than those of the wild type, and excessive formation of apical meristem in the thalli was observed. In addition, the gemma cups of the Mpdwf5a-ko were incomplete, and only a limited number of gemma formations were observed. Treatment with 1 µM of castasterone or 6-deoxocastasterone, a bioactive brassinosteroid (BR), partly restored some of these abnormal phenotypes, but far from complete recovery. These results indicate that MpDWF5A is essential for the normal growth and development of M. polymorpha and suggest that the dwarfism caused by the Mpdwf5a-ko defect is due to the deficiency of typical phytosterols and, in part, a BR-like compound derived from phytosterols.

  Oxford University Press (OUP), May 2023, Plant & Cell Physiology, 64(7) (7), 826 - 838, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Sucrose Signaling Contributes to the Maintenance of Vascular Cambium by Inhibiting Cell Differentiation.

  Aoi Narutaki, Prihardi Kahar, Shunji Shimadzu, Shota Maeda, Tomoyuki Furuya, Kimitsune Ishizaki, Hidehiro Fukaki, Chiaki Ogino, Yuki Kondo

  Plants produce sugars by photosynthesis and use them for growth and development. Sugars are transported from source-to-sink organs via the phloem in the vasculature. It is well known that vascular development is precisely controlled by plant hormones and peptide hormones. However, the role of sugars in the regulation of vascular development is poorly understood. In this study, we examined the effects of sugars on vascular cell differentiation using a vascular cell induction system named Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL). We found that sucrose has the strongest inhibitory effect on xylem differentiation among several types of sugars. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation from cambial cells. Physiological and genetic analysis suggested that sucrose might function through the BES1 transcription factor, which is the central regulator of vascular cell differentiation. Conditional overexpression of cytosolic invertase led to a decrease in the number of cambium layers due to an imbalance between cell division and differentiation. Taken together, our results suggest that sucrose potentially acts as a signal that integrates environmental conditions with the developmental program.

  May 2023, Plant & Cell Physiology, English, Domestic magazine

  [Refereed]

  Scientific journal


• Abscisic acid‐mediated sugar responses are essential for vegetative desiccation tolerance in the liverwort Marchantia polymorpha

  Nobiza Khatun, Akihisa Shinozawa, Kosaku Takahashi, Hideyuki Matsuura, Akida Jahan, Mousona Islam, Masudul Karim, Rahul Sk, Mikako Yoshikawa, Kimitsune Ishizaki, Yoichi Sakata, Daisuke Takezawa

  Wiley, Mar. 2023, Physiologia Plantarum, 175(2) (2), e13898, English

  [Refereed]

  Scientific journal


• G protein signaling and metabolic pathways as evolutionarily conserved mechanisms to combat calcium deficiency

  Richalynn Leong, Javier Jingheng Tan, Sally Shuxian Koh, Ting‐Ying Wu, Kimitsune Ishizaki, Daisuke Urano

  Wiley, Jan. 2023, New Phytologist, 237(2) (2), 615 - 630

  [Refereed]

  Scientific journal


• R2R3-MYB transcription factor GEMMA CUP-ASSOCIATED MYB1 mediates the cytokinin signal to achieve proper organ development in Marchantia polymorpha

  Shiori S. Aki, Tomoyo Morimoto, Taiki Ohnishi, Ayumi Oda, Hirotaka Kato, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Masaaki Umeda

  Abstract Cytokinin, a plant hormone, plays essential roles in organ growth and development. The type-B response regulator-mediated cytokinin signaling is repressed by type-A response regulators and is conserved in the liverwort Marchantia polymorpha. Its signal coordinates the development of diverse organs on the thallus body, such as the gemma cup, rhizoid, and air pores. Here we report that the type-B response regulator MpRRB upregulates the expression of the R2R3-MYB transcription factor GEMMA CUP-ASSOCIATED MYB1 (MpGCAM1) in M. polymorpha. Whereas both Mpgcam1 and Mprrb knockout mutants exhibited defects in gemma cup formation, the Mpgcam1 Mprra double mutant, in which cytokinin signaling is activated due to the lack of type-A response regulator, also formed no gemma cups. This suggests that MpGCAM1 functions downstream of cytokinin signaling. Inducible overexpression of MpGCAM1 produced undifferentiated cell clumps on the thalli of both wild-type and Mprrb. However, smaller thalli were formed in Mprrb compared to the wild-type after the cessation of overexpression. These results suggest that cytokinin signaling promotes gemma cup formation and cellular reprogramming through MpGCAM1, while cytokinin signals also participate in activating cell division during thallus development.

  Springer Science and Business Media LLC, Dec. 2022, Scientific Reports, 12(1) (1), 21123, English

  [Refereed]

  Scientific journal


• G protein controls stress readiness by modulating transcriptional and metabolic homeostasis in Arabidopsis thaliana and Marchantia polymorpha

  Ting-Ying Wu, Shalini Krishnamoorthi, Kulaporn Boonyaves, Isam Al-Darabsah, Richalynn Leong, Alan M. Jones, Kimitsune Ishizaki, Kang-Ling Liao, Daisuke Urano

  Elsevier BV, Dec. 2022, Molecular Plant, 15(12) (12), 1889 - 1907, English

  [Refereed]

  Scientific journal


• The Renaissance and Enlightenment of Marchantia as a model system.

  John L Bowman, Mario Arteaga-Vazquez, Frederic Berger, Liam N Briginshaw, Philip Carella, Adolfo Aguilar-Cruz, Kevin M Davies, Tom Dierschke, Liam Dolan, Ana E Dorantes-Acosta, Tom J Fisher, Eduardo Flores-Sandoval, Kazutaka Futagami, Kimitsune Ishizaki, Rubina Jibran, Takehiko Kanazawa, Hirotaka Kato, Takayuki Kohchi, Jonathan Levins, Shih-Shun Lin, Hirofumi Nakagami, Ryuichi Nishihama, Facundo Romani, Sebastian Schornack, Yasuhiro Tanizawa, Masayuki Tsuzuki, Takashi Ueda, Yuichiro Watanabe, Katsuyuki T Yamato, Sabine Zachgo

  The liverwort Marchantia polymorpha has been utilized as a model for biological studies since the 18th century. In the past few decades there has been a Renaissance in its utilization in genomic and genetic approaches to investigating physiological, developmental, and evolutionary aspects of land plant biology. The reasons for its adoption are similar to those of other genetic models, e.g. simple cultivation, ready access via its worldwide distribution, ease of crossing, facile genetics, and more recently, efficient transformation, genome editing, and genomic resources. The haploid gametophyte dominant life cycle of M. polymorpha is conducive to forward genetic approaches. The lack of ancient whole-genome duplications within liverworts facilitates reverse genetic approaches, and possibly related to this genomic stability, liverworts possess sex chromosomes that evolved in the ancestral liverwort. As a representative of one of the three bryophyte lineages, its phylogenetic position allows comparative approaches to provide insights into ancestral land plants. Given the karyotype and genome stability within liverworts, the resources developed for M. polymorpha have facilitated the development of related species as models for biological processes lacking in M. polymorpha.

  Aug. 2022, The Plant Cell, 34(10) (10), 3512 - 3542, English, International magazine

  [Refereed]

  Scientific journal


• Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverworts

  Akida Jahan, Yuto Yamazaki, Mousona Islam, Totan Kumar Ghosh, Nami Yoshimura, Hirotaka Kato, Kimitsune Ishizaki, Akihisa Shinozawa, Yoichi Sakata, Daisuke Takezawa

  Phytohormone abscisic acid (ABA) plays a key role in stomata closure, osmostress acclimation, and vegetative and embryonic dormancy. Group B3 Raf protein kinases (B3-Rafs) serve as positive regulators of ABA and osmostress signaling in the moss Physcomitrium patens and the angiosperm Arabidopsis thaliana. While P. patens has a single B3-Raf called ARK, specific members of B3-Rafs among six paralogs regulate ABA and osmostress signaling in A. thaliana, indicating functional diversification of B3-Rafs in angiosperms. However, we found that the liverwort Marchantia polymorpha, belonging to another class of bryophytes, has three paralogs of B3-Rafs, MpARK1, MpARK2, and MpARK3, with structural variations in the regulatory domains of the polypeptides. By reporter assays of the P. patens ark line and analysis of genome-editing lines of M. polymorpha, we found that these B3-Rafs are functionally redundant in ABA response, with respect to inhibition of growth, tolerance to desiccation and expression of stress-associated transcripts, the majority of which are under the control of the PYR/PYL/RCAR-like receptor MpPYL1. Interestingly, gemmae in gemma cups were germinating only in mutant lines associated with MpARK1, indicating that dormancy in the gametophyte is controlled by a specific B3-Raf paralog. These results indicated not only conservation of the role of B3-Rafs in ABA and osmostress response in liverworts but also functional diversification of B3-Rafs, which is likely to have occurred in the early stages of land plant evolution.

  Frontiers Media SA, Jul. 2022, Frontiers in Plant Science, 13, 952820, English

  [Refereed]

  Scientific journal


• Distinct Functions of the Atypical Terminal Hydrophilic Domain of the HKT Transporter in the Liverwort Marchantia polymorpha.

  Shahin Imran, Masumi Oyama, Rie Horie, Natsuko I Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie

  K+/Na+ homeostasis is important for land plants, particularly under salt stress. In this study, the structure and ion transport properties of the high-affinity K+ transporter (HKT) of the liverwort Marchantia polymorpha were investigated. Only one HKT gene, MpHKT1, was identified in the genome of M. polymorpha. Phylogenetic analysis of HKT proteins revealed that non-seed plants possess HKTs grouped into a clade independent of the other two clades including HKTs of angiosperms. A distinct long hydrophilic domain was found in the C-terminus of MpHKT1. Complementary DNA (cDNA) of truncated MpHKT1 (t-MpHKT1) encoding the MpHKT_Δ596-812 protein was used to examine the functions of the C-terminal domain. Both MpHKT1 transporters fused with enhanced green fluorescent protein at the N-terminus were localized to the plasma membrane when expressed in rice protoplasts. Two-electrode voltage clamp experiments using Xenopus laevis oocytes indicated that MpHKT1 mediated the transport of monovalent alkali cations with higher selectivity for Na+ and K+, but truncation of the C-terminal domain significantly reduced the transport activity with a decrease in the Na+ permeability. Overexpression of MpHKT1 or t-MpHKT1 in M. polymorpha conferred accumulation of higher Na+ levels and showed higher Na+ uptake rates, compared to those of wild-type plants; however, phenotypes with t-MpHKT1 were consistently weaker than those with MpHKT1. Together, these findings suggest that the hydrophilic C-terminal domain plays a unique role in the regulation of transport activity and ion selectivity of MpHKT1.

  Jun. 2022, Plant & Cell Physiology, 63(6) (6), 802 - 816, English, Domestic magazine

  [Refereed]

  Scientific journal


• Visualization of phosphorus re‐translocation and phosphate transporter expression profiles in a shortened annual cycle system of poplar

  Yuko Kurita, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Ayumi Tezuka, Ayumi Deguchi, Kimitsune Ishizaki, Hidehiro Fukaki, Kei'ichi Baba, Atsushi J. Nagano, Keitaro Tanoi, Tomoko M. Nakanishi, Tetsuro Mimura

  Wiley, Apr. 2022, Plant, Cell & Environment, English

  [Refereed]

  Scientific journal


• A glycogen synthase kinase 3-like kinase MpGSK regulates cell differentiation in Marchantia polymorpha

  Tomoyuki Furuya, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi, Hiroo Fukuda, Yuki Kondo

  Japanese Society for Plant Cell and Molecular Biology, Mar. 2022, Plant Biotechnology, 39(1) (1), 65 - 72

  Scientific journal


• Migration of prospindle before the first asymmetric division in germinating spore of Marchantia polymorpha

  Yuuki Sakai, Takumi Higaki, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Seiichiro Hasezawa

  The development of the plant body starts with spore germination in bryophytes. In many cases, the first division of the spore occurs after germination and cell elongation of the spore. In Marchantia polymorpha, asymmetric division occurs upon spore germination to generate two daughter cells: the larger one retains the ability to divide and develops into the thallus via sporeling or protonema, while the smaller one maintains tip growth and differentiates into the first rhizoid, providing a scaffold for initial development. Although spore germination of M. polymorpha was described in the 19th century, the intracellular processes of the first asymmetric division of the spore have not been well characterized. In this study, we used live-cell imaging analyses to elucidate microtubule dynamics during the first asymmetric division concomitantly with germination. In particular, we demonstrated that the preprophase band was not formed in the spore and that the bipolar prospindle, which is a microtubule structure surrounding the nucleus during prophase, migrated from the center to the periphery in the spore, suggesting that it was the earliest visible sign of cell polarity. We also showed that the occurrence of asymmetric division depended on actin filaments. Our findings regarding the first division of the spore in M. polymorpha will lead to a better model for cell-autonomous asymmetric division in plants.

  Japanese Society for Plant Cell and Molecular Biology, Mar. 2022, Plant Biotechnology, 39(1) (1), 5 - 12, English, Domestic magazine

  [Refereed]

  Scientific journal


• Differential regulation of fluorescent alkaloid metabolism between idioblast and lacticifer cells during leaf development in Catharanthus roseus seedlings.

  Mai Uzaki, Kotaro Yamamoto, Akio Murakami, Yushiro Fuji, Miwa Ohnishi, Kimitsune Ishizaki, Hidehiro Fukaki, Masami Yokota Hirai, Tetsuro Mimura

  Bioactive specialized (secondary) metabolites are indispensable for plant development or adjustment to their surrounding environment. In many plants, these specialized metabolites are accumulated in specifically differentiated cells. Catharanthus roseus is a well-known medicinal plant known for producing many kinds of monoterpenoid indole alkaloids (MIAs). C. roseus has two types of specifically differentiated cells accumulating MIAs, so-called idioblast cells and laticifer cells. In this study, we compared each of the cells as they changed during seedling growth, and found that the fluorescent metabolites accumulated in these cells were differentially regulated. Analysis of fluorescent compounds revealed that the fluorescence observed in these cells was emitted from the compound serpentine. Further, we found that the serpentine content of leaves increased as leaves grew. Our findings suggest that idioblast cells and laticifer cells have different biological roles in MIA biosynthesis and its regulation.

  Mar. 2022, Journal of Plant Research, 135(3) (3), 473 - 483, English, Domestic magazine

  [Refereed]

  Scientific journal


• Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha

  Yohei Mizuno, Aino Komatsu, Shota Shimazaki, Satoshi Naramoto, Keisuke Inoue, Xiaonan Xie, Kimitsune Ishizaki, Takayuki Kohchi, Junko Kyozuka

  Abstract KARRIKIN INSENSITIVE2 (KAI2) was first identified as a receptor of karrikins, smoke-derived germination stimulants. KAI2 is also considered a receptor of an unidentified endogenous molecule called the KAI2 ligand. Upon KAI2 activation, signals are transmitted through the degradation of D53/SMXL proteins via MAX2-dependent ubiquitination. Although components in the KAI2-dependent signaling pathway, namely MpKAI2A and MpKAI2B, MpMAX2, and MpSMXL, exist in the genome of the liverwort Marchantia polymorpha, their functions remain unknown. Here, we show that early thallus growth is retarded and gemma dormancy in the dark is suppressed in Mpkai2a and Mpmax2 loss-of-function mutants. These defects are counteracted in Mpkai2a Mpsmxl and Mpmax2 Mpsmxl double mutants indicating that MpKAI2A, MpMAX2, and MpSMXL act in the same genetic pathway. Introduction of MpSMXLd53, in which a domain required for degradation is mutated, into wild-type plants mimicks Mpkai2a and Mpmax2 plants. In addition, the detection of citrine fluorescence in Nicotiana benthamiana cells transiently expressing a SMXL-Citrine fusion protein requires treatment with MG132, a proteasome inhibitor. These findings imply that MpSMXL is subjected to degradation, and that the degradation of MpSMXL is crucial for MpKAI2A-dependent signaling in M. polymorpha. Therefore, we claim that the basic mechanisms in the KAI2-dependent signaling pathway are conserved in M. polymorpha.

  Oxford University Press (OUP), Aug. 2021, The Plant Cell, 33(7) (7), 2395 - 2411, English, International magazine

  [Refereed]

  Scientific journal


• Development and Molecular Genetics of Marchantia polymorpha

  Takayuki Kohchi, Katsuyuki T. Yamato, Kimitsune Ishizaki, Shohei Yamaoka, Ryuichi Nishihama

  Bryophytes occupy a basal position in the monophyletic evolution of land plants and have a life cycle in which the gametophyte generation dominates over the sporophyte generation, offering a significant advantage in conducting genetics. Owing to its low genetic redundancy and the availability of an array of versatile molecular tools, including efficient genome editing, the liverwort Marchantia polymorpha has become a model organism of choice that provides clues to the mechanisms underlying eco-evo-devo biology in plants. Recent analyses of developmental mutants have revealed that key genes in developmental processes are functionally well conserved in plants, despite their morphological differences, and that lineage-specific evolution occurred by neo/subfunctionalization of common ancestral genes. We suggest that M. polymorpha is an excellent platform to uncover the conserved and diversified mechanisms underlying land plant development. Expected final online publication date for the Annual Review of Plant Biology, Volume 72 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

  Annual Reviews, May 2021, Annual Review of Plant Biology, 72(1) (1), 677 - 702, English

  [Refereed][Invited]

  Scientific journal


• Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation

  Félix Rico-Reséndiz, Sergio Alan Cervantes-Pérez, Annie Espinal-Centeno, Melissa Dipp-Álvarez, Araceli Oropeza-Aburto, Enrique Hurtado-Bautista, Andrés Cruz-Hernández, John L. Bowman, Kimitsune Ishizaki, Mario A. Arteaga-Vázquez, Luis Herrera-Estrella, Alfredo Cruz-Ramírez

  Phosphate (Pi) is a pivotal nutrient that constraints plant development and productivity in natural ecosystems. Land colonization by plants, more than 470 million years ago, evolved adaptive mechanisms to conquer Pi-scarce environments. However, little is known about the molecular basis underlying such adaptations at early branches of plant phylogeny. To shed light on how early divergent plants respond to Pi limitation, we analyzed the morpho-physiological and transcriptional dynamics of Marchantia polymorpha upon Pi starvation. Our phylogenomic analysis highlights some gene networks present since the Chlorophytes and others established in the Streptophytes (e.g., PHR1–SPX1 and STOP1–ALMT1, respectively). At the morpho-physiological level, the response is characterized by the induction of phosphatase activity, media acidification, accumulation of auronidins, reduction of internal Pi concentration, and developmental modifications of rhizoids. The transcriptional response involves the induction of MpPHR1, Pi transporters, lipid turnover enzymes, and MpMYB14, which is an essential transcription factor for auronidins biosynthesis. MpSTOP2 up-regulation correlates with expression changes in genes related to organic acid biosynthesis and transport, suggesting a preference for citrate exudation. An analysis of MpPHR1 binding sequences (P1BS) shows an enrichment of this cis regulatory element in differentially expressed genes. Our study unravels the strategies, at diverse levels of organization, exerted by M. polymorpha to cope with low Pi availability.

  MDPI AG, Nov. 2020, International Journal of Molecular Sciences, 21(21) (21), 8354 - 8354, English

  [Refereed]

  Scientific journal


• Induction of Multichotomous Branching by CLAVATA Peptide in Marchantia polymorpha

  Yuki Hirakawa, Toko Fujimoto, Sakiko Ishida, Naoyuki Uchida, Shinichiro Sawa, Tomohiro Kiyosue, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, John L. Bowman

  Elsevier BV, Oct. 2020, Current Biology, 30(19) (19), 3833 - 3840.e4, English

  [Refereed]

  Scientific journal


• Gemma cup and gemma development in Marchantia polymorpha

  Hirotaka Kato, Yukiko Yasui, Kimitsune Ishizaki

  Corresponding, Wiley, Oct. 2020, New Phytologist, 228(2) (2), 459 - 465, English

  [Refereed][Invited]

  Scientific journal


• Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106

  Bussarin Wachananawat, Takeshi Kuroha, Yuto Takenaka, Hiroyuki Kajiura, Satoshi Naramoto, Ryusuke Yokoyama, Kimitsune Ishizaki, Kazuhiko Nishitani, Takeshi Ishimizu

  Frontiers Media SA, Jul. 2020, Frontiers in Plant Science, 11

  [Refereed]

  Scientific journal


• Design principles of a minimal auxin response system

  Hirotaka Kato, Sumanth K. Mutte, Hidemasa Suzuki, Isidro Crespo, Shubhajit Das, Tatyana Radoeva, Mattia Fontana, Yoshihiro Yoshitake, Emi Hainiwa, Willy van den Berg, Simon Lindhoud, Kimitsune Ishizaki, Johannes Hohlbein, Jan Willem Borst, D. Roeland Boer, Ryuichi Nishihama, Takayuki Kohchi, Dolf Weijers

  Springer Science and Business Media LLC, May 2020, Nature Plants, 6(5) (5), 473 - 482

  [Refereed]

  Scientific journal


• A conserved regulatory mechanism mediates the convergent evolution of plant shoot lateral organs.

  Satoshi Naramoto, Victor Arnold Shivas Jones, Nicola Trozzi, Mayuko Sato, Kiminori Toyooka, Masaki Shimamura, Sakiko Ishida, Kazuhiko Nishitani, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Liam Dolan, Junko Kyozuka

  Land plant shoot structures evolved a diversity of lateral organs as morphological adaptations to the terrestrial environment, with lateral organs arising independently in different lineages. Vascular plants and bryophytes (basally diverging land plants) develop lateral organs from meristems of sporophytes and gametophytes, respectively. Understanding the mechanisms of lateral organ development among divergent plant lineages is crucial for understanding the evolutionary process of morphological diversification of land plants. However, our current knowledge of lateral organ differentiation mechanisms comes almost entirely from studies of seed plants, and thus, it remains unclear how these lateral structures evolved and whether common regulatory mechanisms control the development of analogous lateral organs. Here, we performed a mutant screen in the liverwort Marchantia polymorpha, a bryophyte, which produces gametophyte axes with nonphotosynthetic scalelike lateral organs. We found that an Arabidopsis LIGHT-DEPENDENT SHORT HYPOCOTYLS 1 and Oryza G1 (ALOG) family protein, named M. polymorpha LATERAL ORGAN SUPRESSOR 1 (MpLOS1), regulates meristem maintenance and lateral organ development in Marchantia. A mutation in MpLOS1, preferentially expressed in lateral organs, induces lateral organs with misspecified identity and increased cell number and, furthermore, causes defects in apical meristem maintenance. Remarkably, MpLOS1 expression rescued the elongated spikelet phenotype of a MpLOS1 homolog in rice. This suggests that ALOG genes regulate the development of lateral organs in both gametophyte and sporophyte shoots by repressing cell divisions. We propose that the recruitment of ALOG-mediated growth repression was in part responsible for the convergent evolution of independently evolved lateral organs among highly divergent plant lineages, contributing to the morphological diversification of land plants.

  Dec. 2019, PLoS Biology, 17(12) (12), e3000560, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• GEMMA CUP-ASSOCIATED MYB1, an ortholog of axillary meristem regulators, is essential in vegetative reproduction in Marchantia polymorpha

  Yukiko Yasui, Shigeyuki Tsukamoto, Tomomi Sugaya, Ryuichi Nishihama, Quan Wang, Hirotaka Kato, Katsuyuki T. Yamato, Hidehiro Fukaki, Tetsuro Mimura, Hiroyoshi Kubo, Klaus Theres, Takayuki Kohchi, Kimitsune Ishizaki

  Elsevier BV, Nov. 2019, Current Biology, 29(23) (23), 3987 - 3995, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha.

  Takuma Hiwatashi, Honzhen Goh, Yukiko Yasui, Li Quan Koh, Hideyuki Takami, Masataka Kajikawa, Hiroyuki Kirita, Takehiko Kanazawa, Naoki Minamino, Taisuke Togawa, Mayuko Sato, Mayumi Wakazaki, Katsushi Yamaguchi, Shuji Shigenobu, Hidehiro Fukaki, Tetsuro Mimura, Kiminori Toyooka, Shinichiro Sawa, Katsuyuki T Yamato, Takashi Ueda, Daisuke Urano, Takayuki Kohchi, Kimitsune Ishizaki

  Many plants can reproduce vegetatively, producing clonal progeny from vegetative cells; however, little is known about the molecular mechanisms underlying this process. Liverwort (Marchantia polymorpha), a basal land plant, propagates asexually via gemmae, which are clonal plantlets formed in gemma cups on the dorsal side of the vegetative thallus [1]. The initial stage of gemma development involves elongation and asymmetric divisions of a specific type of epidermal cell, called a gemma initial, which forms on the floor of the gemma cup [2, 3]. To investigate the regulatory mechanism underlying gemma development, we focused on two allelic mutants in which no gemma initial formed; these mutants were named karappo, meaning "empty." We used whole-genome sequencing of both mutants and molecular genetic analysis to identify the causal gene, KARAPPO (KAR), which encodes a ROP guanine nucleotide exchange factor (RopGEF) carrying a plant-specific ROP nucleotide exchanger (PRONE) catalytic domain. In vitro GEF assays showed that the full-length KAR protein and the PRONE domain have significant GEF activity toward MpROP, the only ROP GTPase in M. polymorpha. Moreover, genetic complementation experiments showed a significant role for the N- and C-terminal variable regions in gemma development. Our investigation demonstrates an essential role for KAR/RopGEF in the initiation of plantlet development from a differentiated cell, which may involve cell-polarity formation and subsequent asymmetric cell division via activation of ROP signaling, implying a similar developmental mechanism in vegetative reproduction of various land plants.

  Oct. 2019, Current Biology, 29(20) (20), 3525 - 3531, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• The complexity of intercellular localisation of alkaloids revealed by single-cell metabolomics.

  Kotaro Yamamoto, Katsutoshi Takahashi, Lorenzo Caputi, Hajime Mizuno, Carlos E Rodriguez-Lopez, Tetsushi Iwasaki, Kimitsune Ishizaki, Hidehiro Fukaki, Miwa Ohnishi, Mami Yamazaki, Tsutomu Masujima, Sarah E O'Connor, Tetsuro Mimura

  Catharanthus roseus is a medicinal plant well known for producing bioactive compounds such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). Although the leaves of this plant are the main source of these antitumour drugs, much remains unknown on how TIAs are biosynthesised from a central precursor, strictosidine, to various TIAs in planta. Here, we have succeeded in showing, for the first time in leaf tissue of C. roseus, cell-specific TIAs localisation and accumulation with 10 μm spatial resolution Imaging mass spectrometry (Imaging MS) and live single-cell mass spectrometry (single-cell MS). These metabolomic studies revealed that most TIA precursors (iridoids) are localised in the epidermal cells, but major TIAs including serpentine and vindoline are localised instead in idioblast cells. Interestingly, the central TIA intermediate strictosidine also accumulates in both epidermal and idioblast cells of C. roseus. Moreover, we also found that vindoline accumulation increases in laticifer cells as the leaf expands. These discoveries highlight the complexity of intercellular localisation in plant specialised metabolism.

  Wiley, Oct. 2019, New Phytologist, 224(2) (2), 848 - 859, English, International magazine

  [Refereed]

  Scientific journal


• Cytokinin Signaling Is Essential for Organ Formation in Marchantia polymorpha.

  Shiori S Aki, Tatsuya Mikami, Satoshi Naramoto, Ryuichi Nishihama, Kimitsune Ishizaki, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Junko Kyozuka, Takayuki Kohchi, Masaaki Umeda

  Cytokinins are known to regulate various physiological events in plants. Cytokinin signaling is mediated by the phosphorelay system, one of the most ancient mechanisms controlling hormonal pathways in plants. The liverwort Marchantia polymorpha possesses all components necessary for cytokinin signaling; however, whether they respond to cytokinins and how the signaling is fine-tuned remain largely unknown. Here, we report cytokinin function in Marchantia development and organ formation. Our measurement of cytokinin species revealed that cis-zeatin is the most abundant cytokinin in Marchantia. We reduced the endogenous cytokinin level by overexpressing the gene for cytokinin oxidase, MpCKX, which inactivates cytokinins, and generated overexpression and knockout lines for type-A (MpRRA) and type-B (MpRRB) response regulators to manipulate the signaling. The overexpression lines of MpCKX and MpRRA, and the knockout lines of MpRRB, shared phenotypes such as inhibition of gemma cup formation, enhanced rhizoid formation and hyponastic thallus growth. Conversely, the knockout lines of MpRRA produced more gemma cups and exhibited epinastic thallus growth. MpRRA expression was elevated by cytokinin treatment and reduced by knocking out MpRRB, suggesting that MpRRA is upregulated by the MpRRB-mediated cytokinin signaling, which is antagonized by MpRRA. Our findings indicate that when plants moved onto land they already deployed the negative feedback loop of cytokinin signaling, which has an indispensable role in organogenesis.

  Aug. 2019, Plant & Cell Physiology, 60(8) (8), 1842 - 1854, English, International magazine

  [Refereed]

  Scientific journal


• Physiological function of photoreceptor UVR8 in UV-B tolerance in the liverwort Marchantia polymorpha.

  Youichi Kondou, Yuta Miyagi, Takeshi Morito, Kenta Fujihira, Wataru Miyauchi, Asami Moriyama, Takuya Terasawa, Sakiko Ishida, Kosei Iwabuchi, Hiroyoshi Kubo, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi

  MAIN CONCLUSION: The physiological importance of MpUVR8 in UV-B resistance and translocation in a UV-B-dependent manner from the cytosol into the nucleus is characterized in Marchantia polymorpha. UV RESISTANCE LOCUS 8 (UVR8) is an ultraviolet-B (UV-B) light receptor functioning for UV-B sensing and tolerance in Arabidopsis thaliana and other species. It is unclear whether UVR8 physiologically functions in UV-B-induced defense responses in Marchantia polymorpha, which belongs to the earliest diverging group of embryophyte lineages. Here, we demonstrate that UVR8 has a physiological function in UV-B tolerance and that there is a UVR8-dependent pathway involved. In addition, a UVR8-independent pathway is revealed. We examine the tissue-specific expression pattern of M. polymorpha UVR8 (MpUVR8), showing that it is highly expressed in the apical notch in thalli and gametangiophores, as well as in antheridial and archegonial heads. Furthermore, Mpuvr8KO plant transformants, in which the MpUVR8 locus was disrupted, were produced and analyzed to understand the physiological and molecular function of MpUVR8. Analysis using these plants indicates the important roles of MpUVR8 and MpUVR8-regulated genes, and of MpUVR8-independent pathways in UV-B tolerance. Subcellular localization of Citrine-fused MpUVR8 in M. polymorpha cells was also investigated. It was found to translocate from the cytosol into the nucleus in response to UV-B irradiation. Our findings indicate strong conservation of the physiological function of UVR8 and the molecular mechanisms for UVR8-dependent signal transduction through regulation of gene expression in embryophytes.

  Springer Nature, May 2019, Planta, 249(5) (5), 1349 - 1364, English, International magazine

  [Refereed]

  Scientific journal


• Control of proliferation in the haploid meristem by CLE peptide signaling in Marchantia polymorpha

  Yuki Hirakawa, Naoyuki Uchida, Yasuka L. Yamaguchi, Ryo Tabata, Sakiko Ishida, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Shinichiro Sawa, John L. Bowman

  Public Library of Science (PLoS), Mar. 2019, PLoS Genetics, 15(3) (3), e1997997 - e1007997, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants

  Asuka Higo, Tomokazu Kawashima, Michael Borg, Mingmin Zhao, Irene López-Vidriero, Hidetoshi Sakayama, Sean A. Montgomery, Hiroyuki Sekimoto, Dieter Hackenberg, Masaki Shimamura, Tomoaki Nishiyama, Keiko Sakakibara, Yuki Tomita, Taisuke Togawa, Kan Kunimoto, Akihisa Osakabe, Yutaka Suzuki, Katsuyuki T. Yamato, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, José M. Franco-Zorrilla, David Twell, Frédéric Berger, Takashi Araki

  Springer Science and Business Media LLC, Dec. 2018, Nature Communications, 9(1) (1), 5283, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Responses of the chloroplast glyoxalase system to high CO2 concentrations.

  Ginga Shimakawa, Kentaro Ifuku, Yuji Suzuki, Amane Makino, Kimitsune Ishizaki, Hiroshi Fukayama, Ryutaro Morita, Katsuhiko Sakamoto, Akiko Nishi, Chikahiro Miyake

  Sugar metabolism pathways such as photosynthesis produce dicarbonyls, e.g. methylglyoxal ( MG), which can cause cellular damage. The glyoxalase ( GLX) system comprises two enzymes GLX1 and GLX2, and detoxifies MG; however, this system is poorly understood in the chloroplast, compared with the cytosol. In the present study, we determined GLX1 and GLX2 activities in spinach chloroplasts, which constituted 40% and 10%, respectively, of the total leaf glyoxalase activity. In Arabidopsis thaliana, five GFP-fusion GLXs were present in the chloroplasts. Under high CO2 concentrations, where increased photosynthesis promotes the MG production, GLX1 and GLX2 activities in A. thaliana increased and the expression of AtGLX1-2 and AtGLX2-5 was enhanced. On the basis of these findings and the phylogeny of GLX in oxygenic phototrophs, we propose that the GLX system scavenges MG produced in chloroplasts during photosynthesis.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Dec. 2018, Bioscience, Biotechnology, and Biochemistry, 82(12) (12), 2072 - 2083, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Cryopreservation of Marchantia polymorpha spermatozoa

  Taisuke Togawa, Tohru Adachi, Daijiro Harada, Tasuku Mitani, Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato

  Springer Nature America, Inc, Nov. 2018, Journal of Plant Research, 131(6) (6), 1047 - 1054, English

  [Refereed]

  Scientific journal


• An evolutionary conserved abscisic acid signaling pathway regulates dormancy in the liverwort Marchantia polymorpha

  D. Magnus Eklund, Masakazu Kanei, Eduardo Flores-Sandoval, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Ulf Lagercrantz, Rishikesh P. Bhalerao, Yoichi Sakata, John L. Bowman

  Elsevier {BV}, Nov. 2018, Current Biology, 28(22) (22), 3691 - 3699, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Biosynthesis of riccionidins and marchantins is regulated by R2R3-MYB transcription factors in Marchantia polymorpha

  Hiroyoshi Kubo, Shunsuke Nozawa, Takuma Hiwatashi, Youichi Kondou, Ryo Nakabayashi, Tetsuya Mori, Kazuki Saito, Kojiro Takanashi, Takayuki Kohchi, Kimitsune Ishizaki

  R2R3-MYB transcription factors constitute the largest gene family among plant transcription factor families. They became largely divergent during the evolution of land plants and regulate various biological processes. The functions of R2R3-MYBs are mostly characterized in seed plants but are poorly understood in non-seed plants. Here, we examined the function of two R2R3-MYB genes of Marchantia polymorpha (Mapoly0073s0038 and Mapoly0006s0226) that are closely related to subgroup 4 of the R2R3-MYB family. We performed LC/MS/MS metabolomics, RNA-seq analysis and expression analysis in overexpressors and knockout mutants of MpMYB14 and MpMYB02. Overexpression of MpMYB14 remarkably increased the amount of riccionidins, which are specific anthocyanins in liverworts and a few flowering plants. In contrast, overexpression of MpMYB02 increased the amount of several marchantins, which are characteristic cyclic bis (bibenzyl ether) compounds in M. polymorpha and related liverworts. Knockouts of MpMYB14 and MpMYB02 abolished the accumulation of riccionidins and marchantins, respectively. The expression of MpMYB14 was up-regulated by UV-B irradiation, N deficiency, and NaCl treatment, whereas the expression of MpMYB02 was down-regulated by NaCl treatment. Our results suggest that the regulatory framework of phenolic metabolism by R2R3-MYB was already established in early land plants.

  Last, Springer Tokyo, May 2018, Journal of Plant Research, 131(5) (5), 1 - 16, English, International magazine

  [Refereed]

  Scientific journal


• An evolutionarily conserved NIMA-related kinase directs rhizoid tip growth in the basal land plant Marchantia polymorpha.

  Kento Otani, Kimitsune Ishizaki, Ryuichi Nishihama, Shogo Takatani, Takayuki Kohchi, Taku Takahashi, Hiroyasu Motose

  Tip growth is driven by turgor pressure and mediated by the polarized accumulation of cellular materials. How a single polarized growth site is established and maintained is unclear. Here, we analyzed the function of NIMA-related protein kinase 1 (MpNEK1) in the liverwort Marchantia polymorpha In the wild type, rhizoid cells differentiate from the ventral epidermis and elongate through tip growth to form hair-like protrusions. In Mpnek1 knockout mutants, rhizoids underwent frequent changes in growth direction, resulting in a twisted and/or spiral morphology. The functional MpNEK1-Citrine protein fusion localized to microtubule foci in the apical growing region of rhizoids. Mpnek1 knockouts exhibited increases in both microtubule density and bundling in the apical dome of rhizoids. Treatment with the microtubule-stabilizing drug taxol phenocopied the Mpnek1 knockout. These results suggest that MpNEK1 directs tip growth in rhizoids through microtubule organization. Furthermore, MpNEK1 expression rescued ectopic outgrowth of epidermal cells in the Arabidopsis thaliana nek6 mutant, strongly supporting an evolutionarily conserved NEK-dependent mechanism of directional growth. It is possible that such a mechanism contributed to the evolution of the early rooting system in land plants.

  Mar. 2018, Development (Cambridge, England), 145(5) (5), 1047 - 1054, English, International magazine

  [Refereed]

  Scientific journal


• Generative Cell Specification Requires Transcription Factors Evolutionarily Conserved in Land Plants.

  Shohei Yamaoka, Ryuichi Nishihama, Yoshihiro Yoshitake, Sakiko Ishida, Keisuke Inoue, Misaki Saito, Keitaro Okahashi, Haonan Bao, Hiroyuki Nishida, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T Yamato, Takayuki Kohchi

  Land plants differentiate germ cells in the haploid gametophyte. In flowering plants, a generative cell is specified as a precursor that subsequently divides into two sperm cells in the developing male gametophyte, pollen. Generative cell specification requires cell-cycle control and microtubule-dependent nuclear relocation (reviewed in [1-3]). However, the generative cell fate determinant and its evolutionary origin are still unknown. In bryophytes, gametophytes produce eggs and sperm in multicellular reproductive organs called archegonia and antheridia, respectively, or collectively called gametangia. Given the monophyletic origin of land plants [4-6], evolutionarily conserved mechanisms may play key roles in these diverse reproductive processes. Here, we showed that a single member of the subfamily VIIIa of basic helix-loop-helix (bHLH) transcription factors in the liverwort Marchantia polymorpha primarily accumulated in the initial cells and controlled their development into gametangia. We then demonstrated that an Arabidopsis thaliana VIIIa bHLH transiently accumulated in the smaller daughter cell after an asymmetric division of the meiosis-derived microspore and was required for generative cell specification redundantly with its paralog. Furthermore, these A. thaliana VIIIa bHLHs were functionally replaceable by the M. polymorpha VIIIa bHLH. These findings suggest the VIIIa bHLH proteins as core regulators for reproductive development, including germ cell differentiation, since an early stage of land plant evolution.

  Feb. 2018, Current Biology, 28(3) (3), 479 - 486, English, International magazine

  [Refereed]

  Scientific journal


• DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha

  Nagisa Nagaoka, Akihiro Yamashita, Rina Kurisu, Yuta Watari, Fumiko Ishizuna, Nobuhissro Tsutsumi, Kimitsune Ishizaki, Takayuki Kohchi, Shin-Ichi Arimura

  Mitochondria increase in number by the fission of existing mitochondria. Mitochondrial fission is needed to provide mitochondria to daughter cells during cell division. In Arabidopsis thaliana, four kinds of genes have been reported to be involved in mitochondrial fission. Two of them, DRP3 (dynamin-related protein3) and FIS1 (FISSION1), are well conserved in eukaryotes. The other two are plant-specific ELM1 (elongated mitochondria1) and PMD (peroxisomal and mitochondrial division). To better understand the commonality and diversity of mitochondrial fission factors in land plants, we examined mitochondrial fission-related genes in a liverwort, Marchantia polymorpha. As a bryophyte, M. polymorpha has features distinct from those of the other land plant lineages. We found that M. polymorpha has single copies of homologues for DRP3, FIS1 and ELM1, but does not appear to have a homologue of PMD. Citrine-fusion proteins with MpDRP3, MpFIS1 and MpELM1 were localized to mitochondria in M. polymorpha. MpDRP3-And MpELM1-defective mutants grew slowly and had networked mitochondria, indicating that mitochondrial fission was blocked in the mutants, as expected. However, knockout of MpFIS1 did not affect growth or mitochondrial morphology. These results suggest that MpDRP3 and MpELM1 but neither MpFIS1 nor PMD are needed for mitochondrial fission in M. polymorpha.

  Nature Publishing Group, Dec. 2017, Scientific Reports, 7(1) (1), 4600, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Insights into land plant evolution garnered from the Marchantia polymorpha genome.

  John L Bowman, Takayuki Kohchi, Katsuyuki T Yamato, Jerry Jenkins, Shengqiang Shu, Kimitsune Ishizaki, Shohei Yamaoka, Ryuichi Nishihama, Yasukazu Nakamura, Frédéric Berger, Catherine Adam, Shiori Sugamata Aki, Felix Althoff, Takashi Araki, Mario A Arteaga-Vazquez, Sureshkumar Balasubrmanian, Kerrie Barry, Diane Bauer, Christian R Boehm, Liam Briginshaw, Juan Caballero-Perez, Bruno Catarino, Feng Chen, Shota Chiyoda, Mansi Chovatia, Kevin M Davies, Mihails Delmans, Taku Demura, Tom Dierschke, Liam Dolan, Ana E Dorantes-Acosta, D Magnus Eklund, Stevie N Florent, Eduardo Flores-Sandoval, Asao Fujiyama, Hideya Fukuzawa, Bence Galik, Daniel Grimanelli, Jane Grimwood, Ueli Grossniklaus, Takahiro Hamada, Jim Haseloff, Alexander J Hetherington, Asuka Higo, Yuki Hirakawa, Hope N Hundley, Yoko Ikeda, Keisuke Inoue, Shin-Ichiro Inoue, Sakiko Ishida, Qidong Jia, Mitsuru Kakita, Takehiko Kanazawa, Yosuke Kawai, Tomokazu Kawashima, Megan Kennedy, Keita Kinose, Toshinori Kinoshita, Yuji Kohara, Eri Koide, Kenji Komatsu, Sarah Kopischke, Minoru Kubo, Junko Kyozuka, Ulf Lagercrantz, Shih-Shun Lin, Erika Lindquist, Anna M Lipzen, Chia-Wei Lu, Efraín De Luna, Robert A Martienssen, Naoki Minamino, Masaharu Mizutani, Miya Mizutani, Nobuyoshi Mochizuki, Isabel Monte, Rebecca Mosher, Hideki Nagasaki, Hirofumi Nakagami, Satoshi Naramoto, Kazuhiko Nishitani, Misato Ohtani, Takashi Okamoto, Masaki Okumura, Jeremy Phillips, Bernardo Pollak, Anke Reinders, Moritz Rövekamp, Ryosuke Sano, Shinichiro Sawa, Marc W Schmid, Makoto Shirakawa, Roberto Solano, Alexander Spunde, Noriyuki Suetsugu, Sumio Sugano, Akifumi Sugiyama, Rui Sun, Yutaka Suzuki, Mizuki Takenaka, Daisuke Takezawa, Hirokazu Tomogane, Masayuki Tsuzuki, Takashi Ueda, Masaaki Umeda, John M Ward, Yuichiro Watanabe, Kazufumi Yazaki, Ryusuke Yokoyama, Yoshihiro Yoshitake, Izumi Yotsui, Sabine Zachgo, Jeremy Schmutz

  The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP.

  Oct. 2017, Cell, 171(2) (2), 287 - 304, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• The Roles of the Sole Activator-Type Auxin Response Factor in Pattern Formation of Marchantia polymorpha

  Hirotaka Kato, Masaru Kouno, Mayuko Takeda, Hidemasa Suzuki, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi

  Cell division patterning is important to determine body shape in plants. Nuclear auxin signaling mediated by AUXIN RESPONSE FACTOR (ARF) transcription factors affects plant growth and development through regulation of cell division, elongation and differentiation. The evolutionary origin of the ARF-mediated pathway dates back to at least the common ancestor of bryophytes and other land plants. The liverwort Marchantia polymorpha has three phylogenetically distinct ARFs: MpARF1, the sole 'activator' ARF; and MpARF2 and MpARF3, two 'repressor' ARFs. Genetic screens for auxin-resistant mutants revealed that loss of MpARF1 function conferred auxin insensitivity. Mparf1 mutants showed reduced auxin-inducible gene expression and various developmental defects, including thallus twisting and gemma malformation. We further investigated the role of MpARF1 in gemma development, which is traceable at the cellular level. In wild-type plants, a gemma initial first undergoes several transverse divisions to generate a single-celled stalk and a gemma proper, followed by rather synchronous longitudinal divisions in the latter. Mparf1 mutants often contained multicelled stalks and showed defects in the execution and timing of the longitudinal divisions. While wild-type gemmae finally generate two meristem notches, Mparf1 gemmae displayed various numbers of ectopic meristems. These results suggest that MpARF1 regulates formative cell divisions and axis formation through auxin responses. The mechanism for activator ARF regulation of pattern formation may be shared in land plants and therefore important for the general acquisition of three-dimensional body plans.

  Oxford University Press (OUP), Oct. 2017, Plant & Cell Physiology, 58(10) (10), 1642 - 1651, English, Domestic magazine

  [Refereed]

  Scientific journal


• Inositol hexakis phosphate is the seasonal phosphorus reservoir in the deciduous woody plant Populus alba L.

  Yuko Kurita, Kei'ichi Baba, Miwa Ohnishi, Ryosuke Matsubara, Keiko Kosuge, Aya Anegawa, Chizuko Shichijo, Kimitsune Ishizaki, Yasuko Kaneko, Masahiko Hayashi, Toshinobu Suzaki, Hidehiro Fukaki, Tetsuro Mimura

  Seasonal recycling of nutrients is an important strategy for deciduous perennials. Deciduous perennials maintain and expand their nutrient pools by the autumn nutrient remobilization and the subsequent winter storage throughout their long life. Phosphorus (P), one of the most important elements in living organisms, is remobilized from senescing leaves during autumn in deciduous trees. However, it remains unknown how phosphate is stored over winter. Here we show that in poplar trees (Populus alba L.), organic phosphates are accumulated in twigs from late summer to winter, and that IP6 (myo-inositol-1,2,3,4,5,6-hexakis phosphate: phytic acid) is the primary storage form. IP6 was found in high concentrations in twigs during winter and quickly decreased in early spring. In parenchyma cells of winter twigs, P was associated with electron-dense structures, similar to globoids found in seeds of higher plants. Various other deciduous trees were also found to accumulate IP6 in twigs during winter. We conclude that IP6 is the primary storage form of P in poplar trees during winter, and that it may be a common strategy for seasonal P storage in deciduous woody plants.

  OXFORD UNIV PRESS, Sep. 2017, Plant & Cell Physiology, 58(9) (9), 1477 - 1485, English

  [Refereed]

  Scientific journal


• Diversity of strategies for escaping reactive oxygen species production within photosystem I among land plants: P700 oxidation system is prerequisite for alleviating photoinhibition in photosystem I

  Daisuke Takagi, Kimitsune Ishizaki, Hitomi Hanawa, Tomohito Mabuchi, Ginga Shimakawa, Hiroshi Yamamoto, Chikahiro Miyake

  In land plants, photosystem I (PSI) photoinhibition limits carbon fixation and causes growth defects. In addition, recovery from PSI photoinhibition takes much longer than PSII photoinhibition when the PSI core-complex is degraded by oxidative damage. Accordingly, PSI photoinhibition should be avoided in land plants, and land plants should have evolved mechanisms to prevent PSI photoinhibition. However, such protection mechanisms have not yet been identified, and it remains unclear whether all land plants suffer from PSI photoinhibition in the same way. In the present study, we focused on the susceptibility of PSI to photoinhibition and investigated whether mechanisms of preventing PSI photoinhibition varied among land plant species. To assess the susceptibility of PSI to photoinhibition, we used repetitive short-pulse (rSP) illumination, which specifically induces PSI photoinhibition. Subsequently, we found that land plants possess a wide variety of tolerance mechanisms against PSI photoinhibition. In particular, gymnosperms, ferns and mosses/liverworts exhibited higher tolerance to rSP illumination-induced PSI photoinhibition than angiosperms, and detailed analyses indicated that the tolerance of these groups could be partly attributed to flavodiiron proteins, which protected PSI from photoinhibition by oxidizing the PSI reaction center chlorophyll (P700) as an electron acceptor. Furthermore, we demonstrate, for the first time, that gymnosperms, ferns and mosses/liverworts possess a protection mechanism against photoinhibition of PSI that differs from that of angiosperms.

  WILEY, Sep. 2017, Physiologia Plantarum, 161(1) (1), 56 - 74, English

  [Refereed]

  Scientific journal


• Land plants drive photorespiration as higher electron-sink: comparative study of post-illumination transient O-2-uptake rates from liverworts to angiosperms through ferns and gymnosperms

  Hitomi Hanawa, Kimitsune Ishizaki, Kana Nohira, Daisuke Takagi, Ginga Shimakawa, Takehiro Sejima, Keiichiro Shaku, Amane Makino, Chikahiro Miyake

  In higher plants, the electron-sink capacity of photorespiration contributes to alleviation of photoinhibition by dissipating excess energy under conditions when photosynthesis is limited. We addressed the question at which point in the evolution of photosynthetic organisms photorespiration began to function as electron sink and replaced the flavodiiron proteins which catalyze the reduction of O-2 at photosystem I in cyanobacteria. Algae do not have a higher activity of photorespiration when CO2 assimilation is limited, and it can therefore not act as an electron sink. Using land plants (liverworts, ferns, gymnosperms, and angiosperms) we compared photorespiration activity and estimated the electron flux driven by photorespiration to evaluate its electron-sink capacity at CO2-compensation point. In vivo photorespiration activity was estimated by the simultaneous measurement of O-2-exchange rate and chlorophyll fluorescence yield. All C3-plants leaves showed transient O-2-uptake after actinic light illumination (post-illumination transient O-2-uptake), which reflects photorespiration activity. Post- illumination transient O-2-uptake rates increased in the order from liverworts to angiosperms through ferns and gymnosperms. Furthermore, photorespiration-dependent electron flux in photosynthetic linear electron flow was estimated from post-illumination transient O-2-uptake rate and compared with the electron flux in photosynthetic linear electron flow in order to evaluate the electron-sink capacity of photorespiration. The electron-sink capacity at the CO2-compensation point also increased in the above order. In gymnosperms photorespiration was determined to be the main electron-sink. C3-C4 intermediate species of Flaveria plants showed photorespiration activity, which intermediate between that of C3- and C4-flaveria species. These results indicate that in the first land plants, liverworts, photorespiration started to function as electron sink. According to our hypothesis, the dramatic increase in partial pressure of O-2 in the atmosphere about 0.4 billion years ago made it possible to drive photorespiration with higher activity in liverworts.

  WILEY, Sep. 2017, Physiologia Plantarum, 161(1) (1), 138 - 149, English

  [Refereed]

  Scientific journal


• Chloroplastic ATP synthase builds up a proton motive force preventing production of reactive oxygen species in photosystem I

  Daisuke Takagi, Katsumi Amako, Masaki Hashiguchi, Hidehiro Fukaki, Kimitsune Ishizaki, Tatsuaki Goh, Yoichiro Fukao, Ryosuke Sano, Tetsuya Kurata, Taku Demura, Shinichiro Sawa, Chikahiro Miyake

  Over-reduction of the photosynthetic electron transport (PET) chain should be avoided, because the accumulation of reducing electron carriers produces reactive oxygen species (ROS) within photosystem I (PSI) in thylakoid membranes and causes oxidative damage to chloroplasts. To prevent production of ROS in thylakoid membranes the H+ gradient (pH) needs to be built up across the thylakoid membranes to suppress the over-reduction state of the PET chain. In this study, we aimed to identify the critical component that stimulates pH formation under illumination in higher plants. To do this, we screened ethyl methane sulfonate (EMS)-treated Arabidopsis thaliana, in which the formation of pH is impaired and the PET chain caused over-reduction under illumination. Subsequently, we isolated an allelic mutant that carries a missense mutation in the -subunit of chloroplastic CF0CF1-ATP synthase, named hope2. We found that hope2 suppressed the formation of pH during photosynthesis because of the high H+ efflux activity from the lumenal to stromal side of the thylakoid membranes via CF0CF1-ATP synthase. Furthermore, PSI was in a more reduced state in hope2 than in wild-type (WT) plants, and hope2 was more vulnerable to PSI photoinhibition than WT under illumination. These results suggested that chloroplastic CF0CF1-ATP synthase adjusts the redox state of the PET chain, especially for PSI, by modulating H+ efflux activity across the thylakoid membranes. Our findings suggest the importance of the buildup of pH depending on CF0CF1-ATP synthase to adjust the redox state of the reaction center chlorophyll P700 in PSI and to suppress the production of ROS in PSI during photosynthesis. Significance Statement pH, as well as ATP supply, is important for the adjustment of the redox state in the photosynthetic electron transport chain in higher plants; however, how pH formation is stimulated during photosynthesis is not yet known. This study aimed to identify the critical component to stimulate pH formation during photosynthesis by using Arabidopsis thaliana mutagenized with ethyl methane sulfonate; chloroplastic CF0CF1-ATP synthase was found to build up pH to protect photosynthetic machineries during photosynthesis.

  WILEY, Jul. 2017, The Plant Journal, 91(2) (2), 306 - 324, English

  [Refereed]

  Scientific journal


• Dynamic reorganization of the endomembrane system during spermatogenesis in Marchantia polymorpha

  Naoki Minamino, Takehiko Kanazawa, Ryuichi Nishihama, Katsuyuki T. Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda

  The processes involved in sexual reproduction have been diversified during plant evolution. Whereas charales, bryophytes, pteridophytes, and some gymnosperms utilize motile sperm as male gametes, in other gymnosperms and angiosperms the immotile sperm cells are delivered to the egg cells through elongated pollen tubes. During formation of the motile sperms, cells undergo a dynamic morphological transformation including drastic changes in shape and the generation of locomotor architecture. The molecular mechanism involved in this process remains mostly unknown. Membrane trafficking fulfills the exchange of various proteins and lipids among single membrane-bound organelles in eukaryotic cells, contributing to various biological functions. RAB GTPases and SNARE proteins are evolutionarily conserved key machineries of membrane trafficking mechanisms, which regulate tethering and fusion of the transport vesicles to target membranes. Our observation of fluorescently tagged plasma membrane-resident SNARE proteins demonstrated that these proteins relocalize to spherical structures during the late stages in spermiogenesis. Similar changes in subcellular localization were also observed for other fluorescently tagged SNARE proteins and a RAB GTPase, which acts on other organelles including the Golgi apparatus and endosomes. Notably, a vacuolar SNARE, MpVAMP71, was localized on the membrane of the spherical structures. Electron microscopic analysis revealed that there are many degradation-related structures such as multi-vesicular bodies, autophagosomes, and autophagic bodies containing organelles. Our results indicate that the cell-autonomous degradation pathway plays a crucial role in the removal of membrane components and the cytoplasm during spermiogenesis of Marchantia polymorpha. This process differs substantially from mammalian spermatogenesis in which phagocytic removal of excess cytoplasm involves neighboring cells.

  SPRINGER JAPAN KK, May 2017, Journal of Plant Research, 130(3) (3), 433 - 441, English

  [Refereed]

  Scientific journal


• Occurrence of brassinosteroids in non-flowering land plants, liverwort, moss, lycophyte and fern

  Takao Yokota, Toshiyuki Ohnishi, Kyomi Shibata, Masashi Asahina, Takahito Nomura, Tomomichi Fujita, Kimitsune Ishizaki, Takayuki Kohchi

  Endogenous brassinosteroids (BRs) in non-flowering land plants were analyzed. BRs were found in a liverwort (Marchantia polymorpha), a moss (Physcomitrella patens), lycophytes (Selaginella moellendorffii and S. uncinata) and 13 fern species. A biologically active BR, castasterone (CS), was identified in most of these non-flowering plants but another biologically active BR, brassinolide, was not. It may be distinctive that levels of CS in non-flowering plants were orders of magnitude lower than those in flowering plants. 22-Hydroxycampesterol and its metabolites were identified in most of the non-flowering plants suggesting that the biosynthesis of BRs via 22-hydroxylation of campesterol occurs as in flowering plants. Phylogenetic analyses indicated that M. polymorpha, P. patens and S. moellendorffii have cytochrome P450s in the CYP85 clans which harbors BR biosynthesis enzymes, although the P450 profiles are simpler as compared with Arabidopsis and rice. Furthermore, these basal land plants were found to have multiple P450s in the CYP72 clan which harbors enzymes to catabolize BRs. These findings indicate that green plants were able to synthesize and inactivate BRs from the land-transition stage. (C) 2016 Elsevier Ltd. All rights reserved.

  PERGAMON-ELSEVIER SCIENCE LTD, Apr. 2017, Phytochemistry, 136, 46 - 55, English

  [Refereed]

  Scientific journal


• The Liverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI

  Ginga Shimakawa, Kimitsune Ishizaki, Shigeyuki Tsukamoto, Moeko Tanaka, Takehiro Sejima, Chikahiro Miyake

  The diffusion efficiency of oxygen in the atmosphere, like that of CO2, is approximately 104 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha, a basal land plant, has habitats where it is exposed to both water and the atmosphere. Furthermore, like cyanobacteria, M. polymorpha has genes encoding flavodiiron proteins (FLV). In cyanobacteria, FLVs mediate oxygen-dependent alternative electron flow (AEF) to suppress the production of reactive oxygen species. Here, we investigated whether FLVs are required for the protection of photosynthesis in M. polymorpha. A mutant deficient in the FLV1 isozyme (Delta MpFlv1) sustained photooxidative damage to photosystem I (PSI) following repetitive short-saturation pulses of light. Compared with the wild type (Takaragaike-1), Delta MpFlv1 showed the same photosynthetic oxygen evolution rate but a lower electron transport rate during the induction phase of photosynthesis. Additionally, the reaction center chlorophyll in PSI, P700, was highly reduced in DMpFlv1 but not in Takaragaike-1. These results indicate that the gene product of MpFlv1 drives AEF to oxidize PSI, as in cyanobacteria. Furthermore, FLV-mediated AEF supports the production of a proton motive force to possibly induce the nonphotochemical quenching of chlorophyll fluorescence and suppress electron transport in the cytochrome b(6)/f complex. After submerging the thalli, a decrease in photosystem II operating efficiency was observed, particularly in Delta MpFlv1, which implies that species living in these sorts of habitats require FLV-mediated AEF.

  AMER SOC PLANT BIOLOGISTS, Mar. 2017, Plant Physiology, 173(3) (3), 1636 - 1647, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Evolution of land plants: Insights from molecular studies on basal lineages

  Kimitsune Ishizaki

  The invasion of the land by plants, or terrestrialization, was one of the most critical events in the history of the Earth. The evolution of land plants included significant transformations in body plans: the emergence of a multicellular diploid sporophyte, transition from gametophyte-dominant to sporophyte-dominant life histories, and development of many specialized tissues and organs, such as stomata, vascular tissues, roots, leaves, seeds, and flowers. Recent advances in molecular genetics in two model basal plants, bryophytes Physcomitrella patens and Marchantia polymorpha, have begun to provide answers to several key questions regarding land plant evolution. This paper discusses the evolution of the genes and regulatory mechanisms that helped drive such significant morphological innovations among land-based plants.

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2017, Bioscience, Biotechnology, and Biochemistry, 81(1) (1), 73 - 80, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Evolutionary analysis of iron (Fe) acquisition system in Marchantia polymorpha

  Jing-Chi Lo, Munkhtsetseg Tsednee, Ying-Chu Lo, Shun-Chung Yang, Jer-Ming Hu, Kimitsune Ishizaki, Takayuki Kohchi, Der-Chuen Lee, Kuo-Chen Yeh

  To acquire appropriate iron (Fe), vascular plants have developed two unique strategies, the reduction-based strategy I of nongraminaceous plants for Fe2+ and the chelation-based strategy II of graminaceous plants for Fe3+. However, the mechanism of Fe uptake in bryophytes, the earliest diverging branch of land plants and dominant in gametophyte generation is less clear. Fe isotope fractionation analysis demonstrated that the liverwort Marchantia polymorpha uses reduction-based Fe acquisition. Enhanced activities of ferric chelate reductase and proton ATPase were detected under Fe-deficient conditions. However, M. polymorpha did not show mugineic acid family phytosiderophores, the key components of strategy II, or the precursor nicotianamine. Five ZIP (ZRT/IRT-like protein) homologs were identified and speculated to be involved in Fe uptake in M. polymorpha. MpZIP3 knockdown conferred reduced growth under Fe-deficient conditions, and MpZIP3 overexpression increased Fe content under excess Fe. Thus, a nonvascular liverwort, M. polymorpha, uses strategy I for Fe acquisition. This system may have been acquired in the common ancestor of land plants and coopted from the gametophyte to sporophyte generation in the evolution of land plants.

  WILEY, Jul. 2016, New Phytologist, 211(2) (2), 569 - 583, English

  [Refereed]

  Scientific journal


• An Evolutionarily Conserved Plant RKD Factor Controls Germ Cell Differentiation

  Satoshi Koi, Tetsuya Hisanaga, Katsutoshi Sato, Masaki Shimamura, Katsuyuki T. Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Keiji Nakajima

  In contrast to animals, in which the germ cell lineage is established during embryogenesis, plant germ cells are generated in reproductive organs via reprogramming of somatic cells. The factors that control germ cell differentiation and reprogramming in plants are poorly understood. Members of the RKD subfamily of plant-specific RWP-RK transcription factors have been implicated in egg cell formation in Arabidopsis based on their expression patterns and ability to cause an egg-like transcriptome upon ectopic expression [1]; however, genetic evidence of their involvement is lacking, due to possible genetic redundancy, haploid lethality, and the technical difficulty of analyzing egg cell differentiation in angiosperms. Here we analyzed the factors that govern germ cell formation in the liverwort Marchantia polymorpha. This recently revived model bryophyte has several characteristics that make it ideal for studies of germ cell formation, such as low levels of genetic redundancy, readily accessible germ cells, and the ability to propagate asexually via gemma formation [2, 3]. Our analyses revealed that MpRKD, a single RWP-RK factor closely related to angiosperm RKDs, is preferentially expressed in developing eggs and sperm precursors in M. polymorpha. Targeted disruption of MpRKD had no effect on the gross morphology of the vegetative and reproductive organs but led to striking defects in egg and sperm cell differentiation, demonstrating that MpRKD is an essential regulator of germ cell differentiation. Together with previous findings [1, 4-6], our results suggest that RKD factors are evolutionarily conserved regulators of germ cell differentiation in land plants.

  CELL PRESS, Jul. 2016, Current Biology : CB, 26(13) (13), 1775 - 1781, English

  [Refereed]

  Scientific journal


• Phytochrome Signaling Is Mediated by PHYTOCHROME INTERACTING FACTOR in the Liverwort Marchantia polymorpha

  Keisuke Inoue, Ryuichi Nishihama, Hideo Kataoka, Masashi Hosaka, Ryo Manabe, Mika Nomoto, Yasuomi Tada, Kimitsune Ishizaki, Takayuki Kohchi

  Phytochromes are red light (R) and far-red light (FR) receptors that play important roles in many aspects of plant growth and development. Phytochromes mainly function in the nucleus and regulate sets of genes by inhibiting negatively acting basic helix-loop-helix transcription factors named PHYTOCHROME INTERACTING FACTORs (PIFs) in Arabidopsis thaliana. Although R/FR photoreversible responses and phytochrome genes are well documented in diverse lineages of plants, the extent to which phytochrome signaling is mediated by gene regulation beyond angiosperms remains largely unclear. Here, we show that the liverwort Marchantia polymorpha, an emerging model basal land plant, has only one phytochrome gene, Mp-PHY, and only one PIF gene, Mp-PIF. These genes mediate typical low fluence responses, which are reversibly elicited by R and FR, and regulate gene expression. Mp-phy is light-stable and translocates into the nucleus upon irradiation with either R or FR, demonstrating that the single phytochrome Mp-phy exhibits combined biochemical and cell-biological characteristics of type I and type II phytochromes. Mp-phy photoreversibly regulates gemma germination and downstream gene expression by interacting with Mp-PIF and targeting it for degradation in an R-dependent manner. Our findings suggest that the molecular mechanisms for light-dependent transcriptional regulation mediated by PIF transcription factors were established early in land plant evolution.

  AMER SOC PLANT BIOLOGISTS, Jun. 2016, The Plant Cell, 28(6) (6), 1406 - 1421, English

  [Refereed]

  Scientific journal


• Cell-specific localization of alkaloids in Catharanthus roseus stem tissue measured with Imaging MS and Single-cell MS

  Kotaro Yamamoto, Katsutoshi Takahashi, Hajime Mizuno, Aya Anegawa, Kimitsune Ishizaki, Hidehiro Fukaki, Miwa Ohnishi, Mami Yamazaki, Tsutomu Masujima, Tetsuro Mimura

  Catharanthus roseus (L.) G. Don is a medicinal plant well known for producing antitumor drugs such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). The TIA metabolic pathway in C. roseus has been extensively studied. However, the localization of TIA intermediates at the cellular level has not been demonstrated directly. In the present study, the metabolic pathway of TIA in C. roseus was studied with two forefront metabolomic techniques, that is, Imaging mass spectrometry (MS) and live Single-cell MS, to elucidate cell-specific TIA localization in the stem tissue. Imaging MS indicated that most TIAs localize in the idioblast and laticifer cells, which emit blue fluorescence under UV excitation. Single-cell MS was applied to four different kinds of cells [idioblast (specialized parenchyma cell), laticifer, parenchyma, and epidermal cells] in the stem longitudinal section. Principal component analysis of Imaging MS and Single-cell MS spectra of these cells showed that similar alkaloids accumulate in both idioblast cell and laticifer cell. From MS/MS analysis of Single-cell MS spectra, catharanthine, ajmalicine, and strictosidine were found in both cell types in C. roseus stem tissue, where serpentine was also accumulated. Based on these data, we discuss the significance of TIA synthesis and accumulation in the idioblast and laticifer cells of C. roseus stem tissue.

  NATL ACAD SCIENCES, Apr. 2016, Proceedings of the National Academy of Sciences of the United States of America, 113(14) (14), 3891 - 3896, English

  [Refereed]

  Scientific journal


• Abscisic acid-induced gene expression in the liverwort Marchantia polymorpha is mediated by evolutionarily conserved promoter elements

  Totan K. Ghosh, Midori Kaneko, Khaleda Akter, Shuhei Murai, Kenji Komatsu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Daisuke Takezawa

  Abscisic acid (ABA) is a phytohormone widely distributed among members of the land plant lineage (Embryophyta), regulating dormancy, stomata closure and tolerance to environmental stresses. In angiosperms (Magnoliophyta), ABA-induced gene expression is mediated by promoter elements such as the G-box-like ACGT-core motifs recognized by bZIP transcription factors. In contrast, the mode of regulation by ABA of gene expression in liverworts (Marchantiophyta), representing one of the earliest diverging land plant groups, has not been elucidated. In this study, we used promoters of the liverwort Marchantia polymorpha dehydrin and the wheat Em genes fused to the -glucuronidase (GUS) reporter gene to investigate ABA-induced gene expression in liverworts. Transient assays of cultured cells of Marchantia indicated that ACGT-core motifs proximal to the transcription initiation site play a role in the ABA-induced gene expression. The RY sequence recognized by B3 transcriptional regulators was also shown to be responsible for the ABA-induced gene expression. In transgenic Marchantia plants, ABA treatment elicited an increase in GUS expression in young gemmalings, which was abolished by simultaneous disruption of the ACGT-core and RY elements. ABA-induced GUS expression was less obvious in mature thalli than in young gemmalings, associated with reductions in sensitivity to exogenous ABA during gametophyte growth. In contrast, lunularic acid, which had been suggested to function as an ABA-like substance, had no effect on GUS expression. The results demonstrate the presence of ABA-specific response mechanisms mediated by conserved cis-regulatory elements in liverworts, implying that the mechanisms had been acquired in the common ancestors of embryophytes.

  WILEY-BLACKWELL, Apr. 2016, Physiologia Plantarum, 156(4) (4), 407 - 420, English

  [Refereed]

  Scientific journal


• Cryopreservation of Gemmae from the LiverwortMarchantia polymorphaL.

  Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato

  The liverwort Marchantia polymorpha L. is one of the key model plants in evo-devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, thereby enabling the clonal preservation and rapid propagation of transgenic or mutant lines. Here, we report a simple cryopreservation protocol for in vitro grown M. polymorpha gemmae using aluminum cryoplates. Gemmae were pre-cultured on sucrose-containing medium, embedded in calcium alginate gel on the surface of a cryoplate, moderately dehydrated and stored in liquid nitrogen. After rapid thawing, the stored gemmae showed a 100% survival rate. Our protocol does not require plant growth regulators such as ABA, and takes only 1 h to complete except for 1 d of pre-culture. Furthermore, gemmae treated as described above but then air-dried for 2 h can be stored at -80A degrees C for at least 1 year without a significant decrease in survival rate, which is convenient for most laboratories that have a -80A degrees C freezer but not a liquid nitrogen container for long-term storage. These preservation techniques for M. polymorpha should increase their availability in the research community.

  Oxford University Press (OUP), Feb. 2016, Plant & Cell Physiology, 57(2) (2), 300 - 306, English

  [Refereed]

  Scientific journal


• The Naming of Names: Guidelines for Gene Nomenclature in Marchantia.

  John L Bowman, Takashi Araki, Mario A Arteaga-Vazquez, Frederic Berger, Liam Dolan, Jim Haseloff, Kimitsune Ishizaki, Junko Kyozuka, Shih-Shun Lin, Hideki Nagasaki, Hirofumi Nakagami, Keiji Nakajima, Yasukazu Nakamura, Kyoko Ohashi-Ito, Shinichiro Sawa, Masaki Shimamura, Roberto Solano, Hirokazu Tsukaya, Takashi Ueda, Yuichiro Watanabe, Katsuyuki T Yamato, Sabine Zachgo, Takayuki Kohchi

  While Marchantia polymorpha has been utilized as a model system to investigate fundamental biological questions for over almost two centuries, there is renewed interest in M. polymorpha as a model genetic organism in the genomics era. Here we outline community guidelines for M. polymorpha gene and transgene nomenclature, and we anticipate that these guidelines will promote consistency and reduce both redundancy and confusion in the scientific literature.

  Oxford University Press ({OUP}), Feb. 2016, Plant & Cell Physiology, 57(2) (2), 257 - 61, English, Domestic magazine

  [Refereed]

  Scientific journal


• Transcriptional Framework of Male Gametogenesis in the Liverwort Marchantia polymorpha L.

  Asuka Higo, Masaki Niwa, Katsuyuki T. Yamato, Lixy Yamada, Hitoshi Sawada, Tomoaki Sakamoto, Tetsuya Kurata, Makoto Shirakawa, Motomu Endo, Shuji Shigenobu, Katsushi Yamaguchi, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Takashi Araki

  In land plants, there are two types of male gametes: one is a non-motile sperm cell which is delivered to the egg cell by a pollen tube, and the other is a motile sperm cell with flagella. The molecular mechanism underlying the sexual reproduction with the egg and pollen-delivered sperm cell is well understood from studies using model plants such as Arabidopsis and rice. On the other hand, the sexual reproduction with motile sperm has remained poorly characterized, due to the lack of suitable models. Marchantia polymorpha L. is a model basal land plant with sexual reproduction involving an egg cell and bi-flagellated motile sperm. To understand the differentiation process of plant motile sperm, we analyzed the gene expression profile of developing antheridia of M. polymorpha. We performed RNA-sequencing experiments and compared transcript profiles of the male sexual organ (antheridiophore and antheridium contained therein), female sexual organ (archegoniophore) and a vegetative organ (thallus). Transcriptome analysis showed that the antheridium expresses nearly half of the protein-coding genes predicted in the genome, but it also has unique features. The antheridium transcriptome shares some common features with male gamete transcriptomes of angiosperms and animals, and homologs of genes involved in male gamete formation and function in angiosperms and animals were identified. In addition, we showed that some of them had distinct expression patterns in the spermatogenous tissue of developing antheridia. This study provides a transcriptional framework on which to study the molecular mechanism of plant motile sperm development in M. polymorpha as a model.

  OXFORD UNIV PRESS, Feb. 2016, Plant & Cell Physiology, 57(2) (2), 325 - 338, English

  [Refereed]

  Scientific journal


• SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery

  Takehiko Kanazawa, Atsuko Era, Naoki Minamino, Yu Shikano, Masaru Fujimoto, Tomohiro Uemura, Ryuichi Nishihama, Katsuyuki T. Yamato, Kimitsune Ishizaki, Tomoaki Nishiyama, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda

  The membrane trafficking pathway has been diversified in a specific way for each eukaryotic lineage, probably to fulfill specific functions in the organisms. In green plants, comparative genomics has supported the possibility that terrestrialization and/or multicellularization could be associated with the elaboration and diversification of membrane trafficking pathways, which have been accomplished by an expansion of the numbers of genes required for machinery components of membrane trafficking, including soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. However, information regarding membrane trafficking pathways in basal land plant lineages remains limited. In the present study, we conducted extensive analyses of SNARE molecules, which mediate membrane fusion between target membranes and transport vesicles or donor organelles, in the liverwort, Marchantia polymorpha. The M. polymorpha genome contained at least 34 genes for 36 SNARE proteins, comprising fundamental sets of SNARE proteins that are shared among land plant lineages with low degrees of redundancy. We examined the subcellular distribution of a major portion of these SNARE proteins by expressing Citrine-tagged SNARE proteins in M. polymorpha, and the results showed that some of the SNARE proteins were targeted to different compartments from their orthologous products in Arabidopsis thaliana. For example, MpSYP12B was localized to the surface of the oil body, which is a unique organelle in liverworts. Furthermore, we identified three VAMP72 members with distinctive structural characteristics, whose N-terminal extensions contain consensus sequences for N-myristoylation. These results suggest that M. polymorpha has acquired unique membrane trafficking pathways associated with newly acquired machinery components during evolution.

  OXFORD UNIV PRESS, Feb. 2016, Plant & Cell Physiology, 57(2) (2), 307 - 324, English

  [Refereed]

  Scientific journal


• Profiling and Characterization of Small RNAs in the Liverwort, Marchantia polymorpha, Belonging to the First Diverged Land Plants

  Masayuki Tsuzuki, Ryuichi Nishihama, Kimitsune Ishizaki, Yukio Kurihara, Minami Matsui, John L. Bowman, Takayuki Kohchi, Takahiro Hamada, Yuichiro Watanabe

  MicroRNAs (miRNAs) have important roles in gene regulation during plant development. Previous studies revealed that some miRNAs are highly shared by most land plants. Recently, the liverwort, Marchantia polymorpha, has been studied by molecular genetic approaches, and sequencing of its genome is currently underway. The expression pattern and the detailed functions of miRNAs during Marchantia development are unknown. Here, we profiled the small RNAs expressed in thalli, antheridiophores and archegoniophores of M. polymorpha using high-throughput RNA sequencing. We revealed that a limited number of miRNAs are shared between M. polymorpha and the moss, Physcomitrella patens, and that a number of miRNAs are M. polymorpha specific. Like other land plants, cognate target genes corresponding to conserved miRNAs could be found in the genome database and were experimentally confirmed to guide cleavage of target mRNAs. The results suggested that two genes in the SPL (SQUAMOSA PROMOTER BINDING-LIKE) transcription factor family, which are regulated by miR156 in most land plants, were instead targeted by two distinct miRNAs in M. polymorpha. In order to demonstrate the physiological roles of miRNAs in M. polymorpha, we constructed an miRNA ectopic expression system to establish overexpression transformants for conserved miRNAs, miR166 and miR319. Ectopic expression of these miRNAs induced abnormal development of the thallus and gemma cups, suggesting that balanced expression of miRNA/target mRNAs has a crucial role in developmental regulation in M. polymorpha. Profiling data on miRNA together with the ectopic expression system would provide new information on the liverwort small RNA world and evolutionary divergence/conservation of small RNA function among land plants.

  OXFORD UNIV PRESS, Feb. 2016, Plant & Cell Physiology, 57(2) (2), 359 - 372, English

  [Refereed]

  Scientific journal


• Molecular Genetic Tools and Techniques for Marchantia polymorpha Research

  Kimitsune Ishizaki, Ryuichi Nishihama, Katsuyuki T. Yamato, Takayuki Kohchi

  Liverworts occupy a basal position in the evolution of land plants, and are a key group to address a wide variety of questions in plant biology. Marchantia polymorpha is a common, easily cultivated, dioecious liverwort species, and is emerging as an experimental model organism. The haploid gametophytic generation dominates the diploid sporophytic generation in its life cycle. Genetically homogeneous lines in the gametophyte generation can be established easily and propagated through asexual reproduction, which aids genetic and biochemical experiments. Owing to its dioecy, male and female sexual organs are formed in separate individuals, which enables crossing in a fully controlled manner. Reproductive growth can be induced at the desired times under laboratory conditions, which helps genetic analysis. The developmental process from a single-celled spore to a multicellular body can be observed directly in detail. As a model organism, molecular techniques for M. polymorpha are well developed; for example, simple and efficient protocols of Agrobacterium-mediated transformation have been established. Based on them, various strategies for molecular genetics, such as introduction of reporter constructs, overexpression, gene silencing and targeted gene modification, are available. Herein, we describe the technologies and resources for reverse and forward genetics in M. polymorpha, which offer an excellent experimental platform to study the evolution and diversity of regulatory systems in land plants.

  Lead, OXFORD UNIV PRESS, Feb. 2016, Plant & Cell Physiology, 57(2) (2), 262 - 270, English

  [Refereed]

  Scientific journal


• Identification of miRNAs and Their Targets in the Liverwort Marchantia polymorpha by Integrating RNA-Seq and Degradome Analyses

  Pin-Chun Lin, Chia-Wei Lu, Bing-Nan Shen, Guan-Zong Lee, John L. Bowman, Mario A. Arteaga-Vazquez, Li-Yu Daisy Liu, Syuan-Fei Hong, Chu-Fang Lo, Gong-Min Su, Takayuki Kohchi, Kimitsune Ishizaki, Sabine Zachgo, Felix Althoff, Mizuki Takenaka, Katsuyuki T. Yamato, Shih-Shun Lin

  Bryophytes (liverworts, hornworts and mosses) comprise the three earliest diverging lineages of land plants (embryophytes). Marchantia polymorpha, a complex thalloid Marchantiopsida liverwort that has been developed into a model genetic system, occupies a key phylogenetic position. Therefore, M. polymorpha is useful in studies aiming to elucidate the evolution of gene regulation mechanisms in plants. In this study, we used computational, transcriptomic, small RNA and degradome analyses to characterize microRNA (miRNA)-mediated pathways of gene regulation in M. polymorpha. The data have been integrated into the open access ContigViews-miRNA platform for further reference. In addition to core components of the miRNA pathway, 129 unique miRNA sequences, 11 of which could be classified into seven miRNA families that are conserved in embryophytes (miR166a, miR390, miR529c, miR171-3p, miR408a, miR160 and miR319a), were identified. A combination of computational and degradome analyses allowed us to identify and experimentally validate 249 targets. In some cases, the target genes are orthologous to those of other embryophytes, but in other cases, the conserved miRNAs target either paralogs or members of different gene families. In addition, the newly discovered Mpo-miR11707.1 and Mpo-miR11707.2 are generated from a common precursor and target MpARGONAUTE1 (LW1759). Two other newly discovered miRNAs, Mpo-miR11687.1 and Mpo-miR11681.1, target the MADS-box transcription factors MpMADS1 and MpMADS2, respectively. Interestingly, one of the pentatricopeptide repeat (PPR) gene family members, MpPPR_66 (LW9825), the protein products of which are generally involved in various steps of RNA metabolism, has a long stem-loop transcript that can generate Mpo-miR11692.1 to autoregulate MpPPR_66 (LW9825) mRNA. This study provides a foundation for further investigations of the RNA-mediated silencing mechanism in M. polymorpha as well as of the evolution of this gene silencing pathway in embryophytes.

  OXFORD UNIV PRESS, Feb. 2016, Plant & Cell Physiology, 57(2) (2), 339 - 358, English

  [Refereed]

  Scientific journal


• RSL Class I Genes Controlled the Development of Epidermal Structures in the Common Ancestor of Land Plants

  Helene Proust, Suvi Honkanen, Victor A. S. Jones, Giulia Morieri, Helen Prescott, Steve Kelly, Kimitsune Ishizaki, Takayuki Kohchi, Liam Dolan

  The colonization of the land by plants, sometime before 470 million years ago, was accompanied by the evolution tissue systems [1-3]. Specialized structures with diverse functions from nutrient acquisition to reproduction derived from single cells in the outermost layer (epidermis) were important sources of morphological innovation at this time [2, 4, 5]. In extant plants, these structures may be unicellular extensions, such as root hairs or rhizoids [6-9], or multicellular structures, such as asexual propagules or secretory hairs (papillae) [10-12]. Here, we show that a ROOT-HAIR DEFECTIVE SIX-LIKE (RSL) class I basic helix-loop-helix transcription factor positively regulates the development of the unicellular and multicellular structures that develop from individual cells that expand out of the epidermal plane of the liverwort Marchantia polymorpha; mutants that lack MpRSL1 function do not develop rhizoids, slime papillae, mucilage papillae, or gemmae. Furthermore, we discovered that RSL class I genes are also required for the development of multicellular axillary hairs on the gametophyte of the moss Physcomitrella patens. Because class I RSL proteins also control the development of rhizoids in mosses and root hairs in angiosperms [13, 14], these data demonstrate that the function of RSL class I genes was to control the development of structures derived from single epidermal cells in the common ancestor of the land plants. Class I RSL genes therefore controlled the generation of adaptive morphological diversity as plants colonized the land from the water.

  CELL PRESS, Jan. 2016, Current Biology, 26(1) (1), 93 - 99, English

  [Refereed]

  Scientific journal


• Biochemical characterization of allene oxide synthases from the liverwort Marchantia polymorpha and green microalgae Klebsormidium flaccidum provides insight into the evolutionary divergence of the plant CYP74 family

  Takao Koeduka, Kimitsune Ishizaki, Cynthia Mugo Mwenda, Koichi Hori, Yuko Sasaki-Sekimoto, Hiroyuki Ohta, Takayuki Kohchi, Kenji Matsui

  Allene oxide synthases (AOSs) were isolated from liverworts and charophytes. These AOSs exhibited enzymatic properties similar to those of angiosperms but formed a distinct phylogenetic clade. Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) mediate the formation of precursors of jasmonates and carbon-six volatiles, respectively. AOS and HPL utilize fatty acid hydroperoxides and belong to the plant cytochrome P450 74 (CYP74) family that mediates plant defense against herbivores, pathogens, or abiotic stresses. Although members of the CYP74 family have been reported in mosses and other species, the evolution and function of multiple CYP74 genes in plants remain elusive. Here, we show that the liverwort Marchantia polymorpha belongs to a basal group in the evolution of land plants; has two closely related proteins (59 % identity), MpAOS1 and MpAOS2, that are similar to moss PpAOS1 (49 and 47 % identity, respectively); and exhibits AOS activity but not HPL activity. We also found that the green microalgae Klebsormidium flaccidum, consist of multicellular and non-branching filaments, contains an enzyme, KfAOS, that is similar to PpAOS1 (37 % identity), and converts 13-hydroperoxide of linolenic acid to 12-oxo-phytodienoic acid in a coupled reaction with allene oxide cyclase. Phylogenetic analysis showed two evolutionarily distinct clusters. One cluster comprised AOS and HPL from charophytic algae, liverworts, and mosses, including MpAOSs and KfAOS. The other cluster was formed by angiosperm CYP74. Our results suggest that plant CYP74 enzymes with AOS, HPL, and divinyl ether synthase activities have arisen multiple times and in the two different clades, which occurred prior to the divergence of the flowering plant lineage.

  SPRINGER, Nov. 2015, Planta, 242(5) (5), 1175 - 1186, English

  [Refereed]

  Scientific journal


• Abscisic acid induces biosynthesis of bisbibenzyls and tolerance to UV-C in the liverwort Marchantia polymorpha

  Akito Kageyama, Kimitsune Ishizaki, Takayuki Kohchi, Hideyuki Matsuura, Kosaku Takahashi

  Environmental stresses are effective triggers for the biosynthesis of various secondary metabolites in plants, and phytohormones such as jasmonic acid and abscisic acid are known to mediate such responses in flowering plants. However, the detailed mechanism underlying the regulation of secondary metabolism in bryophytes remains unclear. In this study, the induction mechanism of secondary metabolites in the model liverwort Marchantia polymorpha was investigated. Abscisic acid (ABA) and ultraviolet irradiation (UV-C) were found to induce the biosynthesis of isoriccardin C, marchantin C, and riccardin F, which are categorized as bisbibenzyls, characteristic metabolites of liverworts. UV-C led to the significant accumulation of ABA Overexpression of MpABI1, which encodes protein phosphatase 2C (PP2C) as a negative regulator of ABA signaling, suppressed accumulation of bisbibenzyls in response to ABA and UV-C irradiation and conferred susceptibility to UV-C irradiation. These data show that ABA plays a significant role in the induction of bisbibenzyl biosynthesis, which might confer tolerance against UV-C irradiation in M. polymorpha. (C) 2015 Elsevier Ltd. All rights reserved.

  PERGAMON-ELSEVIER SCIENCE LTD, Sep. 2015, Phytochemistry, 117, 547 - 553, English

  [Refereed]

  Scientific journal


• Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha

  Kimitsune Ishizaki, Ryuichi Nishihama, Minoru Ueda, Keisuke Inoue, Sakiko Ishida, Yoshiki Nishimura, Toshiharu Shikanai, Takayuki Kohchi

  We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3xCitrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3xFLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.

  Lead, PUBLIC LIBRARY SCIENCE, Sep. 2015, PLOS ONE, 10(9) (9), e0138876, English

  [Refereed]

  Scientific journal


• Functional analysis of allene oxide cyclase, MpAOC, in the liverwort Marchantia polymorpha

  Yusuke Yamamoto, Jun Ohshika, Tomohiro Takahashi, Kimitsune Ishizaki, Takayuki Kohchi, Hideyuki Matusuura, Kosaku Takahashi

  12-Oxo-phytodienoic acid (OPDA) is an intermediate in jasmonic acid (JA) biosynthesis. OPDA exerts JA-dependent and JA-independent biological effects; therefore, it is considered a signaling molecule in flowering plants. OPDA is induced by bacterial infection and wounding and inhibits growth in the moss Physcomitrella patens. The functions of OPDA and allene oxide cyclase (AOC) in the liverwort Marchantia polymorpha were explored, which represents the most basal lineage of extant land plants. The analysis of OPDA showed that it is present in M. polymorpha and is increased by wounding. OPDA has been suggested to be involved in the response to environmental stresses. Moreover, OPDA showed growth inhibitory activity in M. polymorpha. Nonetheless JA in M. polymorpha was not found in this study. AOC synthesizes OPDA from an unstable allene oxide. A database search of the M. polymorpha genome identified only a putative gene encoding allene oxide cyclase (MpAOC). Recombinant MpAOC showed AOC activity similar to that in flowering plants. MpAOC was localized to chloroplasts, as in flowering plants. Expression of MpAOC was induced by wounding and OPDA treatment, and positive feedback regulation of OPDA was demonstrated in M. polymorpha. Overexpression of MpAOC increased the endogenous OPDA level and suppressed growth in M. polymorpha. These results indicate the role of OPDA as a signaling molecule regulating growth and the response to wounding in the liverwort M. polymorpha. (C) 2015 Elsevier Ltd. All rights reserved.

  PERGAMON-ELSEVIER SCIENCE LTD, Aug. 2015, Phytochemistry, 116, 48 - 56, English

  [Refereed]

  Scientific journal


• Involvement of Ca2+ in Vacuole Degradation Caused by a Rapid Temperature Decrease in Saintpaulia Palisade Cells: A Case of Gene Expression Analysis in a Specialized Small Tissue

  Miwa Ohnishi, Noriaki Kadohama, Yoshihiro Suzuki, Tomoharu Kajiyama, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Hidetoshi Iida, Hideki Kambara, Tetsuro Mimura

  Saintpaulia (African violet) leaves are known to be damaged by a rapid temperature decrease when cold water is applied to the leaf surface; the injury is ascribed to the chloroplast damage caused by the cytosolic pH decrease following the degradation of the vacuolar membrane in the palisade cells. In this report, we present evidence for the involvement of Ca2+ in facilitating the collapse of the vacuolar membrane and in turn in the temperature sensitivity of Saintpaulia leaves. In the presence of a Ca2+ chelator (EGTA) or certain Ca2+ channel inhibitors (Gd3+ or La3+) but not others (verapamil or nifedipine), the pH of the vacuole, monitored through BCECF (20,70-bis(carboxyethyl)-4 or 5-carboxyfluorescein) fluorescence, did not increase in response to a rapid temperature drop. These pharmacological observations are consistent with the involvement of mechanosensitive Ca2+ channels in the collapse of the vacuolar membrane. The high level of expression of an MCA-(Arabidopsis mechanosensitive Ca2+ channel) like gene, a likely candidate for a mechanosensitive Ca2+ channel(s) in plant cells, was confirmed in the palisade tissue in Saintpaulia leaves by using a newly developed method of gene expression analysis for the specialized small tissues.

  OXFORD UNIV PRESS, Jul. 2015, Plant & Cell Physiology, 56(7) (7), 1297 - 1305, English

  [Refereed][Invited]

  Scientific journal


• Diversification of histone H2A variants during plant evolution

  Tomokazu Kawashima, Zdravko J. Lorkovic, Ryuichi Nishihama, Kimitsune Ishizaki, Elin Axelsson, Ramesh Yelagandula, Takayuki Kohchi, Frederic Berger

  Among eukaryotes, the four core histones show an extremely high conservation of their structure and form nucleosomes that compact, protect, and regulate access to genetic information. Nevertheless, in multicellular eukaryotes the two families, histone H2A and histone H3, have diversified significantly in key residues. We present a phylogenetic analysis across the green plant lineage that reveals an early diversification of the H2A family in unicellular green algae and remarkable expansions of H2A variants in flowering plants. We define motifs and domains that differentiate plant H2A proteins into distinct variant classes. In non-flowering land plants, we identify a new class of H2A variants and propose their possible role in the emergence of the H2A.W variant class in flowering plants.

  ELSEVIER SCIENCE LONDON, Jul. 2015, Trends in Plant Science, 20(7) (7), 419 - 425, English

  [Refereed]

  Scientific journal


• Development of schizogenous intercellular spaces in plants

  Kimitsune Ishizaki

  Gas exchange is essential for multicellular organisms. In contrast to the circulatory systems of animals, land plants have tissues with intercellular spaces (ICSs), called aerenchyma, that are critical for efficient gas exchange. Plants form ICSs by two different mechanisms: schizogeny, where localized cell separation creates spaces; and lysogeny, where cells die to create ICSs. In schizogenous ICS formation, specific molecular mechanisms regulate the sites of cell separation and coordinate extensive reorganization of cell walls. Emerging evidence suggests the involvement of extracellular signaling, mediated by peptide ligands and leucine-rich repeat receptor-like kinases, in the regulation of cell wall remodeling during cell separation. Recent work on the liverwort Marchantia polymorpha has demonstrated a critical role for a plasma membrane-associated plant U-box E3 ubiquitin ligase in ICS formation. In this review, I discuss the mechanism of schizogenous ICS formation, focusing on the potential role of extracellular signaling in the regulation of cell separation.

  FRONTIERS RESEARCH FOUNDATION, Jul. 2015, Frontiers in Plant Science, 6, 497, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Correction: Auxin-Mediated Transcriptional System with a Minimal Set of Components Is Critical for Morphogenesis through the Life Cycle in Marchantia polymorpha.

  Kato H, Ishizaki K, Kouno M, Shirakawa M, Bowman JL, Nishihama R, Kohchi T

  Kato H, Ishizaki K, Kouno M, Shirakawa M, Bowman JL, Nishihama R, Kohchi T, PLoS genetics, 2015, vol. 11, no. 6, pp. e1005365

  Jun. 2015, PLoS Genetics, 11(6) (6), e1005365, English

  [Refereed]

  Scientific journal


• Auxin produced by the indole-3-pyruvic acid pathway regulates development and gemmae dormancy in the liverwort Marchantia polymorpha

  D. Magnus Eklund, Kimitsune Ishizaki, Eduardo Flores-Sandoval, Saya Kikuchi, Yumiko Takebayashi, Shigeyuki Tsukamoto, Yuki Hirakawa, Maiko Nonomura, Hirotaka Kato, Masaru Kouno, Rishikesh P. Bhalerao, Ulf Lagercrantz, Hiroyuki Kasahara, Takayuki Kohchi, John L. Bowman

  The plant hormone auxin (indole-3-acetic acid [IAA]) has previously been suggested to regulate diverse forms of dormancy in both seed plants and liverworts. Here, we use loss-and gain-of-function alleles for auxin synthesis-and signaling-related genes, as well as pharmacological approaches, to study how auxin regulates development and dormancy in the gametophyte generation of the liverwort Marchantia polymorpha. We found that M. polymorpha possess the smallest known toolkit for the indole-3-pyruvic acid (IPyA) pathway in any land plant and that this auxin synthesis pathway mainly is active in meristematic regions of the thallus. Previously a Trp-independent auxin synthesis pathway has been suggested to produce a majority of IAA in bryophytes. Our results indicate that the Trp-dependent IPyA pathway produces IAA that is essential for proper development of the gametophyte thallus of M. polymorpha. Furthermore, we show that dormancy of gemmae is positively regulated by auxin synthesized by the IPyA pathway in the apex of the thallus. Our results indicate that auxin synthesis, transport, and signaling, in addition to its role in growth and development, have a critical role in regulation of gemmae dormancy in M. polymorpha.

  Oxford University Press (OUP), Jun. 2015, The Plant Cell, 27(6) (6), 1650 - 1669, English

  [Refereed]

  Scientific journal


• Phytochrome-mediated regulation of cell division and growth during regeneration and sporeling development in the liverwort Marchantia polymorpha

  Ryuichi Nishihama, Kimitsune Ishizaki, Masashi Hosaka, Yoriko Matsuda, Akane Kubota, Takayuki Kohchi

  Light regulates various aspects of development throughout the life cycle of sessile land plants. Photoreceptors, such as the red (R) and far-red (FR) light receptors phytochromes, play pivotal roles in modulating developmental programs. Reflecting high developmental plasticity, plants can regenerate tissues, organs, and whole bodies from varieties of cells. Among land plants, bryophytes exhibit extraordinary competency of regeneration under hormone-free conditions. As an environmental factor, light plays critical roles in regeneration of bryophytes. However, how light regulates regeneration remains unknown. Here we show that using the liverwort Marchantia polymorpha, which contains a single phytochrome gene, the phytochrome regulates re-entry into the cell cycle and cell shape in newly regenerating tissues. Our morphological and cytological observations revealed that S-phase entry of G(1)-arrested epidermal cells around the midrib on the ventral surface of thallus explants was greatly retarded in the dark or under phytochrome-inactive R/FR cycle irradiation conditions, where, nevertheless, small, laterally narrow regenerants were eventually formed. Thus, consistent with earlier descriptions published over a century ago, light is not essential for, but exerts profound effects on regeneration in M. polymorpha. Ventral cells in regenerants grown under R/FR cycle conditions were longer and narrower than those under R cycle. Expression of a constitutively active mutant of M. polymorpha phytochrome allowed regeneration of well grown, widely expanded thalli even in the dark when sugar was supplied, further demonstrating that the phytochrome signal promotes cell proliferation, which is rate-limited by sucrose availability. Similar effects of R and FR irradiation on cell division and elongation were observed in sporelings as well. Thus, besides activation of photosynthesis, major roles of R in regeneration of M. polymorpha are to facilitate proliferation of rounder cells through the phytochrome by mechanisms that are likely to operate in the sporeling.

  SPRINGER JAPAN KK, May 2015, Journal of Plant Research, 128(3) (3), 407 - 421, English

  [Refereed]

  Scientific journal


• Auxin-Mediated Transcriptional System with a Minimal Set of Components Is Critical for Morphogenesis through the Life Cycle in Marchantia polymorpha

  Hirotaka Kato, Kimitsune Ishizaki, Masaru Kouno, Makoto Shirakawa, John L. Bowman, Ryuichi Nishihama, Takayuki Kohchi

  The plant hormone auxin regulates many aspects of plant growth and development. Recent progress in Arabidopsis provided a scheme that auxin receptors, TIR1/AFBs, target transcriptional co-repressors, AUX/IAAs, for degradation, allowing ARFs to regulate transcription of auxin responsive genes. The mechanism of auxin-mediated transcriptional regulation is considered to have evolved around the time plants adapted to land. However, little is known about the role of auxin-mediated transcription in basal land plant lineages. We focused on the liverwort Marchantia polymorpha, which belongs to the earliest diverging lineage of land plants. M. polymorpha has only a single TIR1/AFB (MpTIR1), a single AUX/IAA (MpIAA), and three ARFs (MpARF1, MpARF2, and MpARF3) in the genome. Expression of a dominant allele of MpIAA with mutations in its putative degron sequence conferred an auxin resistant phenotype and repressed auxin-dependent expression of the auxin response reporter proGH3:GUS. We next established a system for DEX-inducible auxin-response repression by expressing the putatively stabilized MpIAA protein fused with the glucocorticoid receptor domain (MpIAAmDII-GR). Repression of auxin responses in proMpIAA: MpIAAmDII-GR plants caused severe defects in various developmental processes, including gemmaling development, dorsiventrality, organogenesis, and tropic responses. Transient transactivation assays showed that the three MpARFs had different transcriptional activities, each corresponding to their phylogenetic classifications. Moreover, MpIAA and MpARF proteins interacted with each other with different affinities. This study provides evidence that pleiotropic auxin responses can be achieved by a minimal set of auxin signaling factors and suggests that the transcriptional regulation mediated by TIR1/AFB, AUX/IAA, and three types of ARFs might have been a key invention to establish body plans of land plants. We propose that M. polymorpha is a good model to investigate the principles and the evolution of auxin-mediated transcriptional regulation and its roles in land plant morphogenesis.

  PUBLIC LIBRARY SCIENCE, May 2015, PLoS Genetics, 11(5) (5), e1005084., English

  [Refereed]

  Scientific journal


• Altered levels of primary metabolites in response to exogenous indole-3-acetic acid in wild type and auxin signaling mutants of Arabidopsis thaliana: A capillary electrophoresis-mass spectrometry analysis

  Aya Anegawa, Miwa Ohnishi, Daisuke Takagi, Chikahiro Miyake, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

  A comprehensive analysis of the levels of primary metabolites in wild type (WT) and several auxin-signaling mutants namely, tir1, slr and arf7 arf19 of Arabidopsis thaliana has been performed using CE-MS, a technique particularly sensitive for the measurement of polar compounds. We first measured the levels of primary metabolites in shoots and roots, most of the analyzed metabolites were found to be quantitatively and qualitatively comparable in WT and three kinds of mutants (tir1, slr and arf7 arf19). Some amino acids such as GABA, Arg, Orn, Val, Thr, Leu and Ile exhibited a unique pattern of distribution between shoots and roots in both WT and the mutants. On the other hand, the mutant slr showed a quite different pattern of metabolites measured in the present study. Subsequently, the responses of primary metabolites to a short-term (60 min) application of exogenous IAA (10(-7), 10(-8) M) in WT and the mutants were characterized. Due to IAA treatments, some amino acids such as GABA in WT roots and Gly and Ala in WT shoots were altered, but not in the mutants. Gln was altered in slr shoots by 10(-7) M IAA treatment. Levels of G6P from the glycolic pathway were altered in WT roots and those of 2PG, 3PG were altered in tir1 shoots in response to IAA treatments. The levels of succinate in TCA cycle were altered by IAA treatments in WT shoots but not in the mutants. IAA treatment inhibited the respiration in WT roots. The suppression of respiration might account for the IAA-dependent alteration of some metabolites. Difference of auxin responses between WT and auxin-signaling mutants suggests that some metabolic processes are under IAA control.

  JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, Mar. 2015, Plant Biotechnology, 32(1) (1), 65 - 79, English

  [Refereed]

  Scientific journal


• Stomatal Guard Cells Co-opted an Ancient ABA-Dependent Desiccation Survival System to Regulate Stomatal Closure

  Christof Lind, Ingo Dreyer, Enrique J. Lopez-Sanjurjo, Katharina von Meyer, Kimitsune Ishizaki, Takayuki Kohchi, Daniel Lang, Yang Zhao, Ines Kreuzer, Khaled A. S. Al-Rasheid, Hans Ronne, Ralf Reski, Jian-Kang Zhu, Dietmar Geiger, Rainer Hedrich

  During the transition from water to land, plants had to cope with the loss of water through transpiration, the inevitable result of photosynthetic CO2 fixation on land [1, 2]. Control of transpiration became possible through the development of a new cell type: guard cells, which form stomata. In vascular plants, stomatal regulation is mediated by the stress hormone ABA, which triggers the opening of the SnR kinase OST1-activated anion channel SLAC1 [3, 4]. To understand the evolution of this regulatory circuit, we cloned both ABA-signaling elements, SLAC1 and OST1, from a charophyte alga, a liverwort, and a moss, and functionally analyzed the channel-kinase interactions. We were able to show that the emergence of stomata in the last common ancestor of mosses and vascular plants coincided with the origin of SLAC1-type channels capable of using the ancient ABA drought signaling kinase OST1 for regulation of stomatal closure.

  CELL PRESS, Mar. 2015, Current Biology, 25(7) (7), 928 - 935, English

  [Refereed]

  Scientific journal


• Cryopreservation of gemmae from the liverwort

  Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato

  Elsevier {BV}, 2015, Cryobiology, 71(3) (3), 560

  [Refereed]


• Arachidonic acid-dependent carbon-eight volatile synthesis from wounded liverwort (Marchantia polymorpha)

  Hirotomo Kihara, Maya Tanaka, Katsuyuki T. Yamato, Akira Horibata, Atsushi Yamada, Sayaka Kita, Kimitsune Ishizaki, Masataka Kajikawa, Hideya Fukuzawa, Takayuki Kohchi, Yoshihiko Akakabe, Kenji Matsui

  Eight-carbon (C8) volatiles, such as 1-octen-3-ol, octan-3-one, and octan-3-ol, are ubiquitously found among fungi and bryophytes. In this study, it was found that the thalli of the common liverwort Marchantia polymorpha, a model plant species, emitted high amounts of C8 volatiles mainly consisting of (R)-1-octen-3-ol and octan-3-one upon mechanical wounding. The induction of emission took place within 40 min. In intact thalli, 1-octen-3-yl acetate was the predominant C8 volatile while tissue disruption resulted in conversion of the acetate to 1-octen-3-ol. This conversion was carried out by an esterase showing stereospecificity to (R)-1-octen-3-yl acetate. From the transgenic line of M. polymorpha (des6(KO)) lacking arachidonic acid and eicosapentaenoic acid, formation of C8 volatiles was only minimally observed, which indicated that arachidonic and/or eicosapentaenoic acids were essential to form C8 volatiles in M. polymorpha. When des6(KO) thalli were exposed to the vapor of 1-octen-3-ol, they absorbed the alcohol and converted it into 1-octen-3-yl acetate and octan-3-one. Therefore, this implied that 1-octen-3-ol was the primary C8 product formed from arachidonic acid, and further metabolism involving acetylation and oxidoreduction occurred to diversify the C8 products. Octan-3-one was only minimally formed from completely disrupted thalli, while it was formed as the most abundant product in partially disrupted thalli. Therefore, it is assumed that the remaining intact tissues were involved in the conversion of 1-octen-3-ol to octan-3-one in the partially disrupted thalli. The conversion was partly promoted by addition of NAD(P)H into the completely disrupted tissues, suggesting an NAD(P)H-dependent oxidoreductase was involved in the conversion. (C) 2014 Elsevier Ltd. All rights reserved.

  PERGAMON-ELSEVIER SCIENCE LTD, Nov. 2014, Phytochemistry, 107, 42 - 49, English

  [Refereed]

  Scientific journal


• Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha

  Aino Komatsu, Mika Terai, Kimitsune Ishizaki, Noriyuki Suetsugu, Hidenori Tsuboi, Ryuichi Nishihama, Katsuyuki T. Yamato, Masamitsu Wada, Takayuki Kohchi

  Blue-light-induced chloroplast photorelocation movement is observed in most land plants. Chloroplasts move toward weak-light-irradiated areas to efficiently absorb light (the accumulation response) and escape from strong-light-irradiated areas to avoid photodamage (the avoidance response). The plant-specific kinase phototropin (phot) is the blue-light receptor for chloroplast movements. Although the molecular mechanisms for chloroplast photorelocation movement have been analyzed, the overall aspects of signal transduction common to land plants are still unknown. Here, we show that the liverwort Marchantia polymorpha exhibits the accumulation and avoidance responses exclusively induced by blue light as well as specific chloroplast positioning in the dark. Moreover, in silico and Southern-blot analyses revealed that the M. polymorpha genome encodes a single PHOT gene, MpPHOT, and its knockout line displayed none of the chloroplast photorelocation movements, indicating that the sole MpPHOT gene mediates all types of movement. Mpphot was localized on the plasma membrane and exhibited blue-light-dependent autophosphorylation both in vitro and in vivo. Heterologous expression of MpPHOT rescued the defects in chloroplast movement of phot mutants in the fern Adiantum capillus-veneris and the seed plant Arabidopsis (Arabidopsis thaliana). These results indicate that Mpphot possesses evolutionarily conserved regulatory activities for chloroplast photorelocation movement. M. polymorpha offers a simple and versatile platform for analyzing the fundamental processes of phototropin-mediated chloroplast photorelocation movement common to land plants.

  AMER SOC PLANT BIOLOGISTS, Sep. 2014, Plant Physiology, 166(1) (1), 411 - U598, English

  [Refereed]

  Scientific journal


• Co-option of a photoperiodic growth-phase transition system during land plant evolution

  Akane Kubota, Shogo Kita, Kimitsune Ishizaki, Ryuichi Nishihama, Katsuyuki T. Yamato, Takayuki Kohchi

  Photoperiodic control of the phase transition from vegetative to reproductive growth is critical for land plants. The GIGANTEA (GI) and FLAVIN-BINDING KELCH REPEAT F-BOX1 (FKF1) protein complex controls this process in angiosperms. However, little is known about how plants evolved this regulatory system. Here, we report that orthologues of GI and FKF1 are present in a basal plant, the liverwort Marchantia polymorpha, and describe the molecular interaction between their products. Knockout of either the GI or FKF1 orthologue completely abolishes the long-day-dependent growth-phase transition in M. polymorpha. Overexpression of either gene promotes growth-phase transition, even under short-day conditions. Introduction of the GI orthologue partially rescues the late-flowering phenotype of the Arabidopsis thaliana gi mutant. Our findings suggest that plants had already acquired the GI-FKF1 system to regulate growth-phase transition when they colonized land, and that this system was co-opted from gametophyte to sporophyte generation during evolution.

  NATURE PUBLISHING GROUP, Apr. 2014, Nature Communications, 5, 3668, English

  [Refereed]

  Scientific journal


• Comparison of the MpEF1 alpha and CaMV35 promoters for application in Marchantia polymorpha overexpression studies

  Felix Althoff, Sarah Kopischke, Oliver Zobell, Kentaro Ide, Kimitsune Ishizaki, Takayuki Kohchi, Sabine Zachgo

  Constitutive promoters are essential tools for analyses of gene functions by transgenic approaches. For overexpression and silencing studies of genes, a ubiquitous and strong expression of genes under investigation as well as selection markers is preferred. For future applications in the emerging basal plant model system Marchantia polymorpha, a liverwort, activities of the viral 35S cauliflower mosaic virus promoter and the endogenous elongation factor 1 alpha (MpEF1 alpha) promoter were analyzed. Expression of the reporter gene beta-glucuronidase (GUS), driven by the CaMV35 and MpEF1 alpha promoters, was compared throughout plant development. Significant differences were observed between the two promoter activities. The CaMV35 promoter yields a weak reporter gene expression in the meristematic zones but drives a strong expression in the thallus. The MpEF1 alpha promoter causes a strong meristematic GUS expression and is more active in female sexual tissues. Overall, the MpEF1 alpha promoter seems to be the better option for obtaining a strong and ubiquitous transgene expression. Furthermore, a whole mount in situ hybridization protocol for Marchantia was established. Analysis of MpEF1 alpha mRNA transcript in intact, whole tissues showed an expression pattern that is overall similar to the pattern of the GUS reporter gene expression driven by the MpEF1 alpha promoter, including strong expression in meristematic zones. The whole mount technique reported here can be used to determine the mRNA expression in intact gemmae and archegonia, and has the potential to be applied for screening large numbers of transgenic plants, for instance to identify knock-down mutants.

  SPRINGER, Apr. 2014, Transgenic Research, 23(2) (2), 235 - 244, English

  [Refereed]

  Scientific journal


• Essential Role of the E3 Ubiquitin Ligase NOPPERABO1 in Schizogenous Intercellular Space Formation in the Liverwort Marchantia polymorpha

  Kimitsune Ishizaki, Miya Mizutani, Masaki Shimamura, Akihide Masuda, Ryuichi Nishihama, Takayuki Kohchi

  The vast majority of land plants develop gas-exchange tissues with intercellular spaces (ICSs) connected directly to the air. Although the developmental processes of ICS have been described in detail at the morphological and ultrastructural level in diverse land plants, little is known about the molecular mechanism responsible for ICS formation. The liverwort Marchantia polymorpha develops a multilayered tissue with a large ICS (air chamber), whose formation is initiated at selected positions of epidermal cells. We isolated a mutant of M. polymorpha showing impaired air-chamber formation, nopperabo1 (nop1), from T-DNA-tagged lines. In nop1 plants, no ICS was formed; consequently, a single-layered epidermis developed on the dorsal side of the thallus. The causal gene NOP1 encodes a Plant U-box (PUB) E3 ubiquitin ligase carrying tandem ARMADILLO (ARM) repeats in the C terminus. An in vitro ubiquitination assay indicated that the NOP1 protein possesses E3 ubiquitin ligase activity in a U-box-dependent manner. Confocal microscopy and biochemical analysis showed that NOP1 was localized to the plasma membrane. Our investigation demonstrated the essential role of the PUB-ARM-type ubiquitin ligase in ICS formation in M. polymorpha, which sheds light on the molecular mechanism of schizogenous ICS formation in land plants.

  AMER SOC PLANT BIOLOGISTS, Oct. 2013, The Plant Cell, 25(10) (10), 4075 - 4084, English

  [Refereed]

  Scientific journal


• Characterization of four nuclear-encoded plastid RNA polymerase sigma factor genes in the liverwort marchantia polymorpha: Blue-light-and multiple stress-responsive SIG5 was acquired early in the emergence of terrestrial plants

  Takehiko Kanazawa, Kimitsune Ishizaki, Takayuki Kohchi, Mitsumasa Hanaoka, Kan Tanaka

  The plastids of plant cells each contain their own genome, and a bacterial-type RNA polymerase called plastid-encoded plastid RNA polymerase (PEP) is involved in transcription of this genome. While the catalytic core subunits are encoded by the plastid genome, the specificity subunit of PEP, sigma, is generally encoded by the nuclear genome and imported into plastids from the cytoplasm after translation. In this study, we identified and analyzed four sigma factor genes from the nuclear genome of a liverwort, Marchantia polymorpha. Phylogenetic analysis suggested that three of the four genes were orthologous to vascular plant genes and thus they were named MpSIG1, MpSIG2 and MpSIG5. The remaining gene was named MpSIGX. The gene products were predicted to localize to the plastid, and this prediction was experimentally demonstrated by expressing yellow fluorescent protein fusion genes in vivo. As with SIG5 genes of other plant species, expression of MpSIG5 was induced by blue-light irradiation and also under various stress conditions, indicating that the regulatory mechanism responsible is conserved among divergent plant species. However, while the major role of SIG5 in vascular plants is to repair the damaged PSII reaction center through psbD gene transcription, the relevant blue-light-responsive promoter (psbD-BLRP) was not found in M. polymorpha and psbD transcript accumulation did not occur in conjunction with MpSIG5 induction. Thus, the physiological role of SIG5 is probably divergent among plant phyla. © 2013 The Author.

  Oct. 2013, Plant & Cell Physiology, 54(10) (10), 1736 - 1748, English

  [Refereed]

  Scientific journal


• Cold-induced organelle relocation in the liverwort Marchantia polymorpha L.

  Yuka Ogasawara, Kimitsune Ishizaki, Takayuki Kohchi, Yutaka Kodama

  Organelles change their subcellular positions in response to various environmental conditions. Recently, we reported that cold treatments alter the intracellular position of chloroplasts and nuclei (cold positioning) in the fern Adiantum capillus-veneris; chloroplasts and nuclei localized to the periclinal cell wall relocated to anticlinal cell wall after cold treatments. To further understand organelle positioning under cold conditions, we studied cold-induced organelle relocation in the liverwort Marchantia polymorpha L. When sporelings and gemmmalings were treated under low temperature (5 degrees C), chloroplast cold positioning response was successfully induced both in the sporelings and the gemmmalings of M. polymorpha. Using a genetic transformation, nuclei, mitochondria or peroxisomes were visualized with a fluorescent protein, and the transgenic gemmmalings were incubated under the cold condition. Nuclei and peroxisomes, but not mitochondria, clearly relocated from the periclinal cell wall to the anticlinal cell wall after cold treatments. Our findings suggest that several organelles concurrently change their positions in the liverwort cell to cope with cold temperature.

  WILEY-BLACKWELL, Aug. 2013, Plant, Cell & Environment, 36(8) (8), 1520 - 1528, English

  [Refereed]

  Scientific journal


• Subfunctionalization of Sigma Factors during the Evolution of Land Plants Based on Mutant Analysis of Liverwort (Marchantia polymorpha L.) MpSIG1

  Minoru Ueda, Tsuneaki Takami, Lianwei Peng, Kimitsune Ishizaki, Takayuki Kohchi, Toshiharu Shikanai, Yoshiki Nishimura

  Sigma factor is a subunit of plastid-encoded RNA polymerase that regulates the transcription of plastid-encoded genes by recognizing a set of promoters. Sigma factors have increased in copy number and have diversified during the evolution of land plants, but details of this process remain unknown. Liverworts represent the basal group of embryophytes and are expected to retain the ancestral features of land plants. In liverwort (Marchantia polymorpha L.), we isolated and characterized a T-DNA-tagged mutant (Mpsig1) of sigma factor 1 (MpSIG1). The mutant did not show any visible phenotypes, implying that MpSIG1 function is redundant with that of other sigma factors. However, quantitative reverse-transcription polymerase chain reaction and RNA gel blot analysis revealed that genes related to photosynthesis were downregulated, resulting in the minor reduction of some protein complexes. The transcript levels of genes clustered in the petL, psaA, psbB, psbK, and psbE operons of liverwort were lower than those in the wild type, a result similar to that in the SIG1 defective mutant in rice (Oryza sativa). Overexpression analysis revealed primitive functional divergence between the SIG1 and SIG2 proteins in bryophytes, whereas these proteins still retain functional redundancy. We also discovered that the predominant sigma factor for ndhF mRNA expression has been diversified in liverwort, Arabidopsis (Arabidopsis thaliana), and rice. Our study shows the ancestral function of SIG1 and the process of functional partitioning (subfunctionalization) of sigma factors during the evolution of land plants.

  OXFORD UNIV PRESS, 2013, Genome Biology and Evolution, 5(10) (10), 1836 - 1848, English

  [Refereed]

  Scientific journal


• Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.

  Kimitsune Ishizaki, Yasuyo Johzuka-Hisatomi, Sakiko Ishida, Shigeru Iida, Takayuki Kohchi

  The liverwort Marchantia polymorpha is an emerging model organism on account of its ideal characteristics for molecular genetics in addition to occupying a crucial position in the evolution of land plants. Here we describe a method for gene targeting by applying a positive/negative selection system for reduction of non-homologous random integration to an efficient Agrobacterium-mediated transformation system using M. polymorpha sporelings. The targeting efficiency was evaluated by knocking out the NOP1 gene, which impaired air-chamber formation. Homologous recombination was observed in about 2% of the thalli that passed the positive/negative selection. With the advantage of utilizing the haploid gametophytic generation, this strategy should facilitate further molecular genetic analysis of M. polymorpha, in which many of the mechanisms found in land plants are conserved, yet in a less complex form.

  2013, Scientific Reports, 3, 1532, English

  [Refereed]

  Scientific journal


• Efficient Agrobacterium-Mediated Transformation of the Liverwort Marchantia polymorpha Using Regenerating Thalli

  Akane Kubota, Kimitsune Ishizaki, Masashi Hosaka, Takayuki Kohchi

  The thallus, the gametophyte body of the liverwort Marchantia polymorpha, develops clonal progenies called gemmae that are useful in the isolation and propagation of isogenic plants. Developmental timing is critical to Agrobacterium-mediated transformation, and high transformation efficiency has been achieved only with sporelings. Here we report an Agrobacterium-mediated transformation system for M. polymorpha using regenerating thalli. Thallus regeneration was induced by cutting the mature thallus across the apical-basal axis and incubating the basal portion of the thallus for 3d. Regenerating thalli were infected with Agrobacterium carrying binary vector that contained a selection marker, the hygromycin phosphotransferase gene, and hygromycin-resistant transformants were obtained with an efficiency of over 60%. Southern blot analysis verified random integration of 1 to 4 copies of the T-DNA into the M. polymorpha genome. This Agrobacterium-mediated transformation system for M. polymorpha should provide opportunities to perform genetic transformation without preparing spores and to generate a sufficient number of transformants with isogenic background.

  TAYLOR & FRANCIS LTD, Jan. 2013, Bioscience, Biotechnology, and Biochemistry, 77(1) (1), 167 - 172, English

  [Refereed]

  Scientific journal


• Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha

  Minoru Ueda, Tetsuki Kuniyoshi, Hiroshi Yamamoto, Kazuhiko Sugimoto, Kimitsune Ishizaki, Takayuki Kohchi, Yoshiki Nishimura, Toshiharu Shikanai

  The chloroplast NADH dehydrogenase-like (NDH) complex mediates cyclic electron transport and chloro-respiration and consists of five sub-omplexes, which in angiosperms further associate with photosystem I (PSI) to form a super-complex. In Marchantia polymorpha, 11 plastid-encoded subunits and all the nuclear-encoded subunits of the A, B, membrane and ferredoxin-binding sub-complexes are conserved. However, it is unlikely that the genome of this liverwort encodes Lhca5 and Lhca6, both of which mediate NDHPSI super-complex formation. It is also unlikely that the subunits of the lumen sub-complex, PnsL1L4, are encoded by the genome. Consistent with this in silico prediction, the results of blue-native gel electrophoresis showed that NDH subunits were detected in a protein complex with lower molecular mass in Marchantia than the NDHPSI super-complex in Arabidopsis. Using the plastid transformation technique, we knocked out the ndhB gene in Marchantia. Although the wild-type genome copies were completely segregated out, the Delta ndhB lines grew like the wild-type photoautotrophically. A post-illumination transient increase in chlorophyll fluorescence, which reflects NDH activity in vivo in angiosperms, was absent in the thalli of the Delta ndhB lines. In ruptured chloroplasts, antimycin A-insensitive, and ferredoxin-dependent plastoquinone reduction was impaired, suggesting that chloroplast NDH mediates similar electron transport in Marchantia and Arabidopsis, despite its possible difference in structure. As in angiosperms, linear electron transport was not strongly affected in the Delta ndhB lines. However, the plastoquinone pool was slightly more reduced at low light intensity, suggesting that chloroplast NDH functions in redox balancing of the inter system, especially under low light conditions.

  WILEY-BLACKWELL, Nov. 2012, The Plant Journal, 72(4) (4), 683 - 693, English

  [Refereed]

  Scientific journal


• Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha

  Kimitsune Ishizaki, Maiko Nonomura, Hirotaka Kato, Katsuyuki T. Yamato, Takayuki Kohchi

  The phytohormone auxin plays a pivotal role in various developmental aspects in land plants. However, little is known of the auxin response and distribution in non-vascular plants. In this study, we made transgenic plants of the liverwort Marchantia polymorpha which express the uidA (GUS) reporter gene under control of the soybean auxin-inducible promoter, ProGH3, and used it to indirectly monitor auxin-mediated transcriptional activation in planta. Transgenic plants carrying ProGH3:GUS showed GUS activity in an auxin-dependent manner. Histochemical GUS staining was observed at the bottom of gemma cups in the process of vegetative propagation. Significant GUS activity was also detected around the gametophyte-sporophyte junction as well as the developing sporophyte after fertilization. These results suggest that the activity of auxin is crucial in both gametophyte and sporophyte development in M. polymorpha, and that the mechanism for auxin-mediated transcriptional activation had already been established when plants emerged on the terrestrial environment.

  Lead, SPRINGER JAPAN KK, Sep. 2012, Journal of Plant Research, 125(5) (5), 643 - 651, English

  [Refereed]

  Scientific journal


• Characterization of the Plasma Membrane H+-ATPase in the Liverwort Marchantia polymorpha

  Masaki Okumura, Shin-ichiro Inoue, Koji Takahashi, Kimitsune Ishizaki, Takayuki Kohchi, Toshinori Kinoshita

  The plasma membrane H+-ATPase generates an electrochemical gradient of H+ across the plasma membrane that provides the driving force for solute transport and regulates pH homeostasis and membrane potential in plant cells. Recent studies have demonstrated that phosphorylation of the penultimate threonine in H+-ATPase and subsequent binding of a 14-3-3 protein is the major common activation mechanism for H+-ATPase in vascular plants. However, there is very little information on the plasma membrane H+-ATPase in nonvascular plant bryophytes. Here, we show that the liverwort Marchantia polymorpha, which is the most basal lineage of extant land plants, expresses both the penultimate threonine-containing H+-ATPase (pT H+-ATPase) and non-penultimate threonine-containing H+-ATPase (non-pT H+-ATPase) as in the green algae and that pT H+-ATPase is regulated by phosphorylation of its penultimate threonine. A search in the expressed sequence tag database of M. polymorpha revealed eight H+-ATPase genes, designated MpHA (for M. polymorpha H+-ATPase). Four isoforms are the pT H+-ATPase; the remaining isoforms are non-pT H+-ATPase. An apparent 95-kD protein was recognized by anti-H+-ATPase antibodies against an Arabidopsis (Arabidopsis thaliana) isoform and was phosphorylated on the penultimate threonine in response to the fungal toxin fusicoccin in thalli, indicating that the 95-kD protein contains pT H+-ATPase. Furthermore, we found that the pT H(+-)ATPase in thalli is phosphorylated in response to light, sucrose, and osmotic shock and that light-induced phosphorylation depends on photosynthesis. Our results define physiological signals for the regulation of pT H+-ATPase in the liverwort M. polymorpha, which is one of the earliest plants to acquire pT H+-ATPase.

  AMER SOC PLANT BIOLOGISTS, Jun. 2012, Plant Physiology, 159(2) (2), 826 - +, English

  [Refereed]

  Scientific journal


• Analysis of a Range of Catabolic Mutants Provides Evidence That Phytanoyl-Coenzyme A Does Not Act as a Substrate of the Electron-Transfer Flavoprotein/Electron-Transfer Flavoprotein:Ubiquinone Oxidoreductase Complex in Arabidopsis during Dark-Induced Senescence

  Wagner L. Araujo, Kimitsune Ishizaki, Adriano Nunes-Nesi, Takayuki Tohge, Tony R. Larson, Ina Krahnert, Ilse Balbo, Sandra Witt, Peter Doermann, Ian A. Graham, Christopher J. Leaver, Alisdair R. Fernie

  The process of dark-induced senescence in plants is not fully understood, however, the functional involvement of an electron-transfer flavoprotein/electron-transfer flavoprotein: ubiquinone oxidoreductase (ETF/ETFQO), has been demonstrated. Recent studies have revealed that the enzymes isovaleryl-coenzyme A (CoA) dehydrogenase and 2-hydroxyglutarate dehydrogenase act as important electron donors to this complex. In addition both enzymes play a role in the breakdown of cellular carbon storage reserves with isovaleryl-CoA dehydrogenase being involved in degradation of the branched-chain amino acids, phytol, and lysine while 2-hydroxyglutarate dehydrogenase is exclusively involved in lysine degradation. Given that the chlorophyll breakdown intermediate phytanoyl-CoA accumulates dramatically both in knockout mutants of the ETF/ETFQO complex and of isovaleryl-CoA dehydrogenase following growth in extended dark periods we have investigated the direct importance of chlorophyll breakdown for the supply of carbon and electrons during this process. For this purpose we isolated three independent Arabidopsis (Arabidopsis thaliana) knockout mutants of phytanoyl-CoA 2-hydroxylase and grew them under the same extended darkness regime as previously used. Despite the fact that these mutants accumulated phytanoyl-CoA and also 2-hydroxyglutarate they exhibited no morphological changes in comparison to the other mutants previously characterized. These results are consistent with a single entry point of phytol breakdown into the ETF/ETFQO system and furthermore suggest that phytol is not primarily metabolized by this pathway. Furthermore analysis of isovaleryl-CoA dehydrogenase/2-hydroxyglutarate dehydrogenase double mutants generated here suggest that these two enzymes essentially account for the entire electron input via the ETF complex.

  AMER SOC PLANT BIOLOGISTS, Sep. 2011, Plant Physiology, 157(1) (1), 55 - 69, English

  [Refereed]

  Scientific journal


• Protein degradation - an alternative respiratory substrate for stressed plants

  Wagner L. Araujo, Takayuki Tohge, Kimitsune Ishizaki, Christopher J. Leaver, Alisdair R. Fernie

  In cellular circumstances under which carbohydrates are scarce, plants can metabolize proteins and lipids as alternative respiratory substrates. Respiration of protein is less efficient than that of carbohydrate as assessed by the respiratory quotient; however, under certain adverse conditions, it represents an important alternative energy source for the cell. Significant effort has been invested in understanding the regulation of protein degradation in plants. This has included an investigation of how proteins are targeted to the proteosome, and the processes of senescence and autophagy. Here we review these events with particular reference to amino acid catabolism and its role in supporting the tricarboxylic acid cycle and direct electron supply to the ubiquinone pool of the mitochondrial electron transport chain in plants.

  ELSEVIER SCIENCE LONDON, Sep. 2011, Trends in Plant Science, 16(9) (9), 489 - 498, English

  [Refereed]

  Scientific journal


• Identification of the 2-Hydroxyglutarate and Isovaleryl-CoA Dehydrogenases as Alternative Electron Donors Linking Lysine Catabolism to the Electron Transport Chain of Arabidopsis Mitochondria

  Wagner L. Araujo, Kimitsune Ishizaki, Adriano Nunes-Nesi, Tony R. Larson, Takayuki Tohge, Ina Krahnert, Sandra Witt, Toshihiro Obata, Nicolas Schauer, Ian A. Graham, Christopher J. Leaver, Alisdair R. Fernie

  The process of dark-induced senescence in plants is relatively poorly understood, but a functional electron-transfer flavoprotein/electron-transfer flavoprotein: ubiquinone oxidoreductase (ETF/ETFQO) complex, which supports respiration during carbon starvation, has recently been identified. Here, we studied the responses of Arabidopsis thaliana mutants deficient in the expression of isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase to extended darkness and other environmental stresses. Evaluations of the mutant phenotypes following carbon starvation induced by extended darkness identify similarities to those exhibited by mutants of the ETF/ETFQO complex. Metabolic profiling and isotope tracer experimentation revealed that isovaleryl-CoA dehydrogenase is involved in degradation of the branched-chain amino acids, phytol, and Lys, while 2-hydroxyglutarate dehydrogenase is involved exclusively in Lys degradation. These results suggest that isovaleryl-CoA dehydrogenase is the more critical for alternative respiration and that a series of enzymes, including 2-hydroxyglutarate dehydrogenase, plays a role in Lys degradation. Both physiological and metabolic phenotypes of the isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase mutants were not as severe as those observed for mutants of the ETF/ETFQO complex, indicating some functional redundancy of the enzymes within the process. Our results aid in the elucidation of the pathway of plant Lys catabolism and demonstrate that both isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase act as electron donors to the ubiquinol pool via an ETF/ETFQO-mediated route.

  AMER SOC PLANT BIOLOGISTS, May 2010, The Plant Cell, 22(5) (5), 1549 - 1563, English

  [Refereed]

  Scientific journal


• Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

  Ken Tougane, Kenji Komatsu, Salma Begum Bhyan, Yoichi Sakata, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Daisuke Takezawa

  Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the beta-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.

  AMER SOC PLANT BIOLOGISTS, Mar. 2010, Plant Physiology, 152(3) (3), 1529 - 1543, English

  [Refereed]

  Scientific journal


• Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha

  Atsuko Era, Motoki Tominaga, Kazuo Ebine, Chie Awai, Chieko Saito, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda

  Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live imaging of filamentous actin (F-actin) have been developed, but new versatile probes are still needed. Here, we report the application of a new probe called Lifeact for visualizing F-actin in plant cells. Lifeact is a short peptide comprising 17 amino acids that was derived from yeast Abp140p. We used a LifeactVenus fusion protein for staining F-actin in Arabidopsis thaliana and were able to observe dynamic rearrangements of the actin meshwork in root hair cells. We also used LifeactVenus to visualize the actin cytoskeleton in the liverwort Marchantia polymorpha; this revealed unique and dynamic F-actin motility in liverwort cells. Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages.

  OXFORD UNIV PRESS, Jun. 2009, Plant & Cell Physiology, 50(6) (6), 1041 - 1048, English

  [Refereed]

  Scientific journal


• Gene content, organization and molecular evolution of plant organellar genomes and sex chromosomes - Insights from the case of the liverwort Marchantia polymorpha

  Kanji Ohyama, Miho Takemura, Kenji Oda, Hideya Fukuzawa, Takayuki Kohchi, Sigeki Nakayama, Kimitsune Ishizaki, Masaki Fujisawa, Katsuyuki Yamato

  The complete nucleotide sequence of chloroplast DNA (121;025 base pairs, bp) from a liverwort, Marchantia polymorpha, has made clear the entire gene organization of the chloroplast genome. Quite a few genes encoding components of photosynthesis and protein synthesis machinery have been identified by comparative computer analysis. We also determined the complete nucleotide sequence of the liverwort mitochondrial DNA and deduced 96 possible genes in the sequence of 186,608 bp. The complete chloroplast geuome encodes twenty introns (19 group II and I group I) in 18 different, genes. One of the chloroplast group II introns separates a ribosomal protein gene in a trans-position. The mitochondrial genome contains thirty-two introns (25 group II and 7 group I) in the coding regions of 17 genes. From the evolutionary point of view, we describe the origin of organellar introns and give evidence for vertical and horizontal intron transfers and their intragenomic propagation. Furthermore, we describe the gene organization of the Y chromosome in the dioecious liverwort M. polymorpha, the first detailed view of a Y chromosome in a haploid organism. On the 10 megabase (Mb) Y chromosome, 64 genes are identified, 14 of which are detected only in the male genome. These 14 genes are expressed in reproductive organs but not in vegetative thalli, suggesting their participation in male reproductive functions. These findings indicate that the Y and X chromosomes share the same ancestral autosome and support the prediction that in a haploid organism essential genes on sex chromosomes are more likely to persist than in a diploid organism.

  JAPAN ACAD, Mar. 2009, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 85(3) (3), 108 - 124, English

  [Refereed]

  Scientific journal


• Direct transformation of the liverwort Marchantia polymorpha L. by particle bombardment using immature thalli developing from spores

  Shota Chiyoda, Kimitsune Ishizaki, Hideo Kataoka, Katsuyuki T. Yamato, Takayuki Kohchi

  The liverwort, Marchantia polymorpha L., belongs to a group of basal land plants and is an emerging model for plant biology. We established a procedure to prepare sporangia of M. polymorpha under laboratory conditions by promoting its transition to reproductive development by far-red light irradiation. Here we report an improved direct transformation system of M. polymorpha using immature thalli developing from spores. Hygromycin-resistant transformants were obtained on selective media by transformation with a plasmid carrying the hygromycin-phosphotransferase gene (hpt) conferring hygromycin resistance in 4 weeks. The aminoglycoside-3 ''-adenyltransferase gene (aadA) conferring spectinomycin resistance was also successfully used as an additional selectable marker for nuclear transformation of M. polymorpha. The availability of the aadA gene in addition to the hpt gene should make M. polymorpha a versatile host for genetic manipulation. DNA gel-blot analyses indicated that transformed thalli carried a variable number of copies of the transgene integrated into the genome. Although the previous system using thalli grown from gemmae required a two-step selection in liquid and solid media for 8 weeks, the system reported here using thalli developing from spores allows generation of transformants in half the time by direct selection on solid media, facilitating genetic analyses in this model plant.

  SPRINGER, Sep. 2008, Plant Cell Reports, 27(9) (9), 1467 - 1473, English

  [Refereed]

  Scientific journal


• Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology

  Kimitsune Ishizaki, Shota Chiyoda, Katsuyuki T. Yamato, Takayuki Kohchi

  Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that contains a reporter, the beta-glucuronidase (GUS) gene with an intron, and a selection marker, the hygromycin phosphotransferase (hpt) gene. In this system, individual gemmae, which arise asexually from single initial cells, were analyzed as isogenic transformants. GUS activity staining showed that all hygromycin-resistant plants examined expressed the GUS transgene in planta. DNA analyses verified random integration of 1-5 copies of the intact T-DNA between the right and the left borders into the M. polymorpha genome. The efficient and rapid Agrobacterium-mediated transformation of M. polymorpha should provide molecular techniques to facilitate comparative genomics, taking advantage of this unique model plant that retains many features of the common ancestor of land plants.

  Lead, OXFORD UNIV PRESS, Jul. 2008, Plant & Cell Physiology, 49(7) (7), 1084 - 1091, English

  [Refereed]

  Scientific journal


• Gene organization of the liverwort Y chromosome reveals distinct sex chromosome evolution in a haploid system

  Katsuyuki T. Yamato, Kimitsune Ishizaki, Masaki Fujisawa, Sachiko Okada, Shigeki Nakayama, Mariko Fujishita, Hiroki Bando, Kohei Yodoya, Kiwako Hayashi, Tomoyuki Bando, Akiko Hasumi, Tomohisa Nishio, Ryoko Sakata, Masayuki Yamamoto, Arata Yamaki, Masataka Kajikawa, Takashi Yamano, Taku Nishide, Seung-Hyuk Choi, Yuu Shimizu-Ueda, Tsutomu Hanajiri, Megumi Sakaida, Kaoru Kono, Mizuki Takenaka, Shohei Yamaoka, Chiaki Kuriyama, Yoshito Kohzu, Hiroyuki Nishida, Axel Brennicke, Tadasu Shin-i, Yuji Kohara, Takayuki Kohchi, Hideya Fukuzawa, Kanji Ohyama

  Y chromosomes are different from other chromosomes because of a lack of recombination. Until now, complete sequence information of Y chromosomes has been available only for some primates, although considerable information is available for other organisms, e.g., several species of Drosophila. Here, we report the gene organization of the Y chromosome in the dioecious liverwort Marchantia polymorpha and provide a detailed view of a Y chromosome in a haploid organism. On the 10-Mb Y chromosome, 64 genes are identified, 14 of which are detected only in the male genome and are expressed in reproductive organs but not in vegetative thalli, suggesting their participation in male reproductive functions. Another 40 genes on the Y chromosome are expressed in thalli and male sexual organs. At least six of these genes have diverged X-linked counterparts that are in turn expressed in thalli and sexual organs in female plants, suggesting that these X-and Y-linked genes have essential cellular functions. These findings indicate that the Y and X chromosomes share the same ancestral autosome and support the prediction that in a haploid organism essential genes on sex chromosomes are more likely to persist than in a diploid organism.

  NATL ACAD SCIENCES, Apr. 2007, Proceedings of the National Academy of Sciences of the United States of America, 104(15) (15), 6472 - 6477, English

  [Refereed]

  Scientific journal


• The mitochondrial electron transfer flavoprotein complex is essential for survival of Arabidopsis in extended darkness

  Kimitsune Ishizaki, Nicolas Schauer, Tony R. Larson, Ian A. Graham, Alisdair R. Fernie, Christopher J. Leaver

  In mammals, the electron transfer flavoprotein (ETF) is a heterodimeric protein composed of two subunits, alpha and beta, that is responsible for the oxidation of at least nine mitochondrial matrix flavoprotein dehydrogenases. Electrons accepted by ETF are further transferred to the main respiratory chain via the ETF ubiquinone oxide reductase (ETFQO). Sequence analysis of the unique Arabidopsis homologues of two subunits of ETF revealed their high similarity to both subunits of the mammalian ETF. Yeast two-hybrid experiments showed that the Arabidopsis ETF alpha and ETF beta can form a heteromeric protein. Isolation and characterization of two independent T-DNA insertional Arabidopsis mutants of the ETF beta gene revealed accelerated senescence and early death compared to wild-type during extended darkness. Furthermore in contrast to wild-type, the etfb mutants demonstrated a significant accumulation of several amino acids, isovaleryl CoA and phytanoyl CoA during dark-induced carbohydrate deprivation. These phenotypic characteristics of etfb mutants are broadly similar to those that we observed previously in Arabidopsis etfqo mutants, suggesting functional association between ETF and ETFQO in Arabidopsis, and confirming the essential roles of the ETF/ETFQO electron transfer complex in the catabolism of leucine and involvement in the chlorophyll degradation pathway activated during dark-induced carbohydrate deprivation.

  Lead, WILEY-BLACKWELL PUBLISHING, INC, Sep. 2006, The Plant Journal, 47(5) (5), 751 - 760, English

  [Refereed]

  Scientific journal


• The critical role of Arabidopsis electron-transfer flavoprotein: Ubiquinone oxidoreductase during dark-induced starvation

  Kimitsune Ishizaki, Tony R. Larson, Nicolas Schauer, Alisdair R. Fernie, Ian A. Graham, Christopher J. Leaver

  In mammals, electron-transfer flavoprotein: ubiquinone oxidoreductase (ETFQO) and electron-transfer flavoprotein (ETF) are functionally associated, and ETF accepts electrons from at least nine mitochondrial matrix flavoprotein dehydrogenases and transfers them to ubiquinone in the inner mitochondrial membrane. In addition, the mammalian ETF/ETFQO system plays a key role in beta-oxidation of fatty acids and catabolism of amino acids and choline. By contrast, nothing is known of the function of ETF and ETFQO in plants. Sequence analysis of the unique Arabidopsis thaliana homologue of ETFQO revealed high similarity to the mammalian ETFQO protein. Moreover, green fluorescent protein cellular localization experiments suggested a mitochondrial location for this protein. RNA gel blot analysis revealed that Arabidopsis ETFQO transcripts accumulated in long-term dark-treated leaves. Analysis of three independent insertional mutants of Arabidopsis ETFQO revealed a dramatic reduction in their ability to withstand extended darkness, resulting in senescence and death within 10 d after transfer, whereas wild-type plants remained viable for at least 15 d. Metabolite profiling of dark-treated leaves of the wild type and mutants revealed a dramatic decline in sugar levels. In contrast with the wild type, the mutants demonstrated a significant accumulation of several amino acids, an intermediate of Leu catabolism, and, strikingly, high-level accumulation of phytanoyl-CoA. These data demonstrate the involvement of a mitochondrial protein, ETFQO, in the catabolism of Leu and potentially of other amino acids in higher plants and also imply a novel role for this protein in the chlorophyll degradation pathway activated during dark-induced senescence and sugar starvation.

  Lead, AMER SOC PLANT BIOLOGISTS, Sep. 2005, The Plant Cell, 17(9) (9), 2587 - 2600, English

  [Refereed]

  Scientific journal


• Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis

  Vicky Buchanan-Wollaston, Tania Page, Elizabeth Harrison, Emily Breeze, Pyung Ok Lim, Hong Gil Nam, Ji-Feng Lin, Shu-Hsing Wu, Jodi Swidzinski, Kimitsune Ishizaki, Christopher J Leaver

  An analysis of changes in global gene expression patterns during developmental leaf senescence in Arabidopsis has identified more than 800 genes that show a reproducible increase in transcript abundance. This extensive change illustrates the dramatic alterations in cell metabolism that underpin the developmental transition from a photosynthetically active leaf to a senescing organ which functions as a source of mobilizable nutrients. Comparison of changes in gene expression patterns during natural leaf senescence with those identified, when senescence is artificially induced in leaves induced to senesce by darkness or during sucrose starvation-induced senescence in cell suspension cultures, has shown not only similarities but also considerable differences. The data suggest that alternative pathways for essential metabolic processes such as nitrogen mobilization are used in different senescent systems. Gene expression patterns in the senescent cell suspension cultures are more similar to those for dark-induced senescence and this may be a consequence of sugar starvation in both tissues. Gene expression analysis in senescing leaves of plant lines defective in signalling pathways involving salicylic acid (SA), jasmonic acid (JA) and ethylene has shown that these three pathways are all required for expression of many genes during developmental senescence. The JA/ethylene pathways also appear to operate in regulating gene expression in dark-induced and cell suspension senescence whereas the SA pathway is not involved. The importance of the SA pathway in the senescence process is illustrated by the discovery that developmental leaf senescence, but not dark-induced senescence, is delayed in plants defective in the SA pathway.

  BLACKWELL PUBLISHING LTD, May 2005, The Plant Journal, 42(4) (4), 567 - 585, English

  [Refereed]

  Scientific journal


• Evolution of ribosomal DNA unit on the X chromosome independent of autosomal units in the liverwort Marchantia polymorpha

  Masaki Fujisawa, Shigeki Nakayama, Tomohisa Nishio, Mariko Fujishita, Kiwako Hayashi, Kimitsune Ishizaki, Masataka Kajikawa, Katsuyuki T. Yamato, Hideya Fukuzawa, Kanji Ohyama

  In the haploid dioecious liverwort, Marchantia polymorpha, the X chromosome, but not the Y, carries a cluster of ribosomal RNA genes (rDNAs). Here we show that sequences of 5S, 17S, 5.8S and 26S rDNAs are highly conserved(> 99% identity) between the X chromosomal and autosomal rDNA repeat units, but the intergenic spacer sequences differ considerably. The most prominent difference is the presence of a 615-bp DNA fragment in the intergenic spacer, X615, which has accumulated predominantly in the rDNA cluster of the X chromosome. These observations suggest that the rDNA repeat unit on the X chromosome evolved independently of that on autosomes, incorporating sex chromosome-specific sequences.

  KLUWER ACADEMIC PUBL, 2003, Chromosome Research, 11(7) (7), 695 - 703, English

  [Refereed]

  Scientific journal


• Multicopy genes uniquely amplified in the Y chromosome-specific repeats of the liverwort Marchantia polymorpha

  Kimitsune Ishizaki, Yuu Shimizu‐Ueda, Sachiko Okada, Masayuki Yamamoto, Masaki Fujisawa, Katsuyuki T. Yamato, Hideya Fukuzawa, Kanji Ohyama

  Sex of the liverwort Marchantia polymorpha is determined by the sex chromosomes Y and X, in male and female plant, respectively. Approximately half of the Y chromosome is made up of unique repeat sequences. Here, we report that part of the Y chromosome, represented by a 90-kb insert of a genomic clone pMM2D3, contains five putative genes in addition to the ORF162 gene, which is present also within the Y chromosome-specific repeat region. One of the five putative genes shows similarity to a male gamete-specific protein of lily and is expressed predominantly in male sex organs, suggesting that this gene has a male reproductive function. Furthermore, Southern blot analysis revealed that these five putative genes are amplified on the Y chromosome, but they also probably have homologs on the X chromosome and/or autosomes. These observations suggest that the Y chromosome evolved by co-amplifying protein-coding genes with unique repeat sequences.

  Lead, OXFORD UNIV PRESS, Nov. 2002, Nucleic Acids Research, 30(21) (21), 4675 - 4681, English

  [Refereed]

  Scientific journal


• The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene

  Sachiko Okada, Takefumi Sone, Masaki Fujisawa, Shigeki Nakayama, Mizuki Takenaka, Kimitsune Ishizaki, Kaoru Kono, Yuu Shimizu-Ueda, Tsutomu Hanajiri, Katsuyuki T. Yamato, Hideya Fukuzawa, Axel Brennicke, Kanji Ohyama

  The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, pMM4G7 and pMM23-130F12. Several chromosome-specific sequence elements of approximate to 70 to 400 nt are combined into larger arrangements, which in turn are assembled into extensive Y chromosome-specific stretches. These repeat sequences contribute 2-3 Mb to the Y chromosome based on the observations of three different approaches: fluorescence in situ hybridization, dot blot hybridization, and the frequency of clones containing the repeat sequences in the genomic library. A novel Y chromosome-specific gene family was found embedded among these repeat sequences. This gene family encodes a putative protein with a RING finger motif and is expressed specifically in male sexual organs. To our knowledge, there have been no other reports for an active Y chromosome-specific gene in plants. The chromosome-specific repeat sequences possibly contribute to determining the identity of the Y chromosome in M. polymorpha as well as to maintaining genes required for male functions, as in mammals such as human.

  NATL ACAD SCIENCES, Jul. 2001, Proceedings of the National Academy of Sciences of the United States of America, 98(16) (16), 9454 - 9459, English

  [Refereed]

  Scientific journal


• Ccm1, a regulatory gene controlling the induction of a carbon-concentrating mechanism in Chlamydomonas reinhardtii by sensing CO2 availability

  Hideya Fukuzawa, Kenji Miura, Kimitsune Ishizaki, Ken-ichi Kucho, Tatsuaki Saito, Tsutomu Kohinata, Kanji Ohyama

  Aquatic photosynthetic organisms, including the green alga Chlamydomonas reinhardtii, induce a set of genes for a carbon-concentrating mechanism (CCM) to acclimate to CO2-limiting conditions. This acclimation is modulated by some mechanisms in the cell to sense CO2 availability. Previously, a high-CO2-requiring mutant C16 defective in an induction of the CCM was isolated from C,reinhardtii by gene tagging. By using this pleiotropic mutant, we isolated a nuclear regulatory gene, Ccm1, encoding a 699-aa hydrophilic protein with a putative zinc-finger motif in its N-terminal region and a Gin repeat characteristic of transcriptional activators, Introduction of Ccm1 into this mutant restored an active carbon transport through the CCM, development of a pyrenoid structure in the chloroplast. and induction of a set of CCM-related genes. That a 5,128-base Ccm1 transcript and also the translation product of 76 kDa were detected in both high- and low-CO2 conditions suggests that CCM1 might be modified posttranslationally. These data indicate that Ccm1 is essential to control the induction of CCM by sensing CO2 availability in Chlamydomonas cells. In addition, complementation assay and identification of the mutation site of another pleiotropic mutant, cia5, revealed that His-54 within the putative zinc-finger motif of the CCM1 is crucial to its regulatory function.

  NATL ACAD SCIENCES, Apr. 2001, Proceedings of the National Academy of Sciences of the United States of America, 98(9) (9), 5347 - 5352, English

  [Refereed]

  Scientific journal


• Isolation and characterization of high-CO2 requiring mutants from Chlamydomonas reinhardtii by gene tagging

  Hideya Fukuzawa, Kimitsune Ishizaki, Kenji Miura, Shoji Matsueda, Tatsuya Ino-ue, Ken-ichi Kucho, Kanji Ohyama

  To isolate genes essential to the carbon concentrating mechanism and the CO2-signal transduction mechanisms, seven high-CO2 requiring mutants were isolated from Chlamydomonas reinhardtii by the insertion of nit1 gene encoding nitrate reductase. These mutants were grouped into two classes. Class I contains mutants that show a severe CO2-requiring phenotype (C1 and C3). Class II includes those with a moderate CO2-requiring phenotype (C2, C5, C7, C15, and C16). One of the class II mutants, C5, appeared to be defective in the utilization of intracellularly accumulated inorganic carbon. On the other hand, the mutant C16 appeared to be defective not only in the uptake of carbon but also in the induction of the periplasmic carbonic anhydrase gene, CAH1, as well as in the development of pyrenoid structure under low-CO2 conditions. These mutations in C5 and C16 mutants appeared to be caused by single insertional events of the introduced DNA fragment into the genomic DNA, suggesting that their corresponding genes could be tagged using the nit1 gene.

  NATL RESEARCH COUNCIL CANADA, Jun. 1998, Canadian Journal of Botany, 76(6) (6), 1092 - 1097, English

  [Refereed]

  Scientific journal

• Technical platform for metabolic engineering in a bryophyte Marchantia polymorpha

  石崎 公庸, 酒井 友希

  Last, Mar. 2023, アグリバイオ, 7(3) (3), 12 - 15, Japanese

  [Invited]

  Introduction scientific journal


• The bHLH transcription factor MpHYPNOS regulates gemma dormancy in the liverwort Marchantia polymorpha

  Hirotaka Kato, Nami Yoshimura, Mikako Yoshikawa, Hideyuki Matsuura, Kosaku Takahashi, Daisuke Takezawa, Tomoyuki Furuya, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  25 Apr. 2022, bioRxiv, 488978


• 庭の厄介者ゼニゴケがクローン個体をつくり繁殖する仕組み

  安居佑季子, 石崎公庸

  Corresponding, Sep. 2020, 化学と生物, 58(9) (9), 502 - 504, Japanese

  [Refereed]


• Role of E3 ubiquitin ligase in formation of intercellular spaces in plants

  Kimitsune Ishizaki, Takayuki Kohchi

  Ishizaki K, Kohchi T, Seikagaku. The Journal of Japanese Biochemical Society, 2014, vol. 86, no. 4, pp. 508-512

  Lead, Japanese Biochemical Society, Apr. 2014, Seikagaku, 86(4) (4), 508 - 512, Japanese

  [Refereed]

  Book review


• 基部陸上植物の光応答戦略−フィトクロムを介した光形態形成の分子機構−

  ISHIZAKI KIMITSUNE, 井上 佳祐, 河内 孝之

  2013, 植物科学の最前線, Japanese


• ゼニゴケ(研究室の片隅で生き物への愛を語る,生物材料インデックス)

  石崎 公庸, 河内 孝之

  日本生物工学会, 2012, 生物工学会誌 : seibutsu-kogaku kaishi, 90(9) (9), 600 - 603, Japanese

  Introduction scientific journal


• 陸上植物に固有の転写因子LFY

  荒木崇, 酒井友希, 辻井由香, 宇山和樹, 宮下結衣, 川本麻美, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之

  2010, 日本植物学会大会研究発表記録, 74th


• 苔類ゼニゴケにおけるPEBP family遺伝子の機能解析

  竹本覚, 辻井由香, 有手友嗣, 竹村美保, 酒井友希, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  2010, 日本植物学会大会研究発表記録, 74th


• 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの機能解析

  酒井友希, 宮下結衣, 宇山和樹, 辻井由香, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  2010, 日本植物学会大会研究発表記録, 74th


• 新規ゼニゴケ核ゲノム形質転換選抜用マーカーの開発

  上田 実, 石崎 公庸, 大和 勝幸, 河内 孝之, 鹿内 利治, 西村 芳樹

  2010, 日本植物生理学会、 第51回年会、於 熊本大学、2010年3月, Japanese

  [Refereed]


• The basics of cultivating the liverwort Marchantia polymorpha L.

  YAMATO Katsuyuki, ISHIZAKI Kimitsune, KOHCHI Takayuki

  Mar. 2009, Low Temperature Science, 67, 23 - 29, Japanese

  [Invited]


• Agrobacterium-mediated transformation of the liverwort Marchantia polymorpha L.

  石崎 公庸, 河内 孝之

  苔類ゼニゴケは，アグロバクテリウムを介する形質転換が容易である．胞子から培養した分化初期の葉状体を使うことによって，1胞子嚢あたり数百の独立した形質転換体が短期間で作成できる．世代の大半が半数体であることや無性芽による純系化といった特徴を生かしたユニークな実験が可能となった．Here we describe a rapid Agrobacterium-mediated transformation protocol for the liverwort, Marchantia polymorpha L. using immature thalli developed from spores. This protocol should provide molecular techniques to facilitate comparative genomics, taking advantage of this unique model plant that retains many features of the common ancestor of land plants.光合成研究法. 北海道大学低温科学研究所, 日本光合成研究会共編

  北海道大学低温科学研究所 = Institute of Low Temperature Science, Hokkaido University, 2008, Low temperature science, 67, 597 - 600, Japanese

• 植物生理学 第2版

  石崎 公庸

  Contributor, 第１章「植物の起源と進化」, 化学同人, Apr. 2019, Japanese

  Textbook


• ケイン生物学 第5版

  A. Singh-Cundy, M. L. Cain, J. Dusheck, 上村 慎治, ISHIZAKI KIMITSUNE, 赤染 康久, 奥野 誠, 神谷 律, 堂前 雅史, 野口 幸子, 廣野 喜幸, FUKAKI HIDEHIRO, 松田 学, 三浦 徹, MIMURA TETSURO, 吉田 千枝, 和田 祐子

  Joint translation, 東京化学同人, Sep. 2014, Japanese, 「第15章 遺伝子からタンパク質へ」の翻訳を行った。, ISBN: 9784807908523

  Textbook

• Causal gene identification of the air chamber-less mutant zunberabo in the liverwort Marchantia polymorpha

  Ayana Sano, Airi Hayashi, Yuuki Sakai, Kimitsune Ishizaki, Yoh Sakuma, Hirotaka Kato

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Poster presentation


• Regulatory mechanisms of DNA damage response and meristem maintenance via RNA m6A modification

  Ryosuke Matsuo, Akihito Mamiya, Kentaro P. Iwata, Yuuki Sakai, Takuya Sakamoto, Sachihiro Matsunaga, Yuki Kondo, Kimitsune Ishizaki, Hidehiro Fukaki

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• A transient cytokinin response maximum completes bifacial vascular stem cells for radial growth

  Shunji Shimadzu, Takaaki Yonekura, Tomoyuki Furuya, Mikiko Kojima, Kimitsune Ishizaki, Masashi Asahina, Kyoko Ohashi-Ito, Hitoshi Sakakibara, Hidehiro Fukaki, Hiroo Fukuda, Yuki Kondo

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• Auxin-inducible LBD transcription factors suppress shoot regeneration in phytohormone-induced callus

  Akihito Mamiya, Koki Wada, Hatsune Morinaka, Ayako Kawamura, Kotaro Torii, Akira Iwase, Tatsuaki Goh, Yuki Kondo, Kimitsune Ishizaki, Keiko Sugimoto, Hidehiro Fukaki

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• Functional Characterization of a Splicing Variant from the MpHKT1 Gene Encoding a Na+-permeable Channel in the Liverwort Marchantia polymorpha

  Takumi Koen, Chihiro Takegami, Kimitsune Ishizaki, Shahin Imran, Maki Katsuhara, Takayuki Kohchi, Natsuko I. Kobayashi, Keitaro Tanoi, Tomoaki Horie

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Poster presentation


• Phenotypic analyses of the loss-of-function mutants of f- and m-type thioredoxin in Marchantia polymorpha

  Taichi Sugiyama, Yuuki Sakai, Masaru Kono, Kimitsune Ishizaki, Keisuke Yoshida

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Poster presentation


• Morphological and physiological studies on the assimilatory filaments in the liverwort Marchantia polymorpha

  Chihiro Sugiyama, Yuuki Sakai, Ginga Shimakawa, Hidehiro Fukaki, Kimitsune Ishizaki

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Poster presentation


• Analysis of phospholipid degradation mechanism under phosphate starvation in Marchantia polymorpha

  Kota Takahara, Kimitsune Ishizaki, Mie Shimojima

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Poster presentation


• Leaf position and season-dependent changes in transcriptome and ionome analysis of field-grown poplar cuttings

  Yuko Kurita, Makoto Kashima, Kei’ichi Baba, Kimitsune Ishizaki, Natsuko I. Kobayashi, Keitaro Tanoi, Tetsuro Mimura, Atsushi Nagano

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• An R2R3-MYB transcription factor, SHOT GLASS promotes gemma cup development and FR-induced gametangiophore formation in the liverwort Marchantia polymorpha

  Yuuki Sakai, Hideyuki Takami, Shohei Yamaoka, Hirotaka Kato, Tetsuro Mimura, Hidehiro Fukaki, Kimitsune Ishizaki

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• Analysis of leaf position-dependent phosphorus allocation in a deciduous woody plant Populus alba L.

  Aoi Hirata, Yuko Kurita, Kimitsune Ishizaki, Natsuko I. Kobayashi, Keitaro Tanoi

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Poster presentation


• R2R3-MYB transcription factors facilitate regeneration from decapitated explants in the liverwort Marchantia polymorpha

  Yukiko Yasui, Hirotaka Kato, Suzuha Tanaka, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• Evolutionary conserved RLF, a plant cytochrome b5-like heme-binding protein, is essential for proper organ development in Marchantia polymorpha

  Kentaro P. Iwata, Takayuki Shimizu, Yuuki Sakai, Tomoyuki Furuya, Hinatamaru Fukumura, Yuki Kondo, Tatsuru Masuda, Kimitsune Ishizaki, Hidehiro Fukaki

  The 66th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2025, Japanese

  Oral presentation


• ゼニゴケの栄養成長における幹細胞制御〜GCAM1まわりの研究から見えてきたこと〜

  石崎 公庸

  コケ幹細胞研究会2024, Dec. 2024, Japanese

  Public symposium


• Developmental roles of WIP, the evolutionarily conserved auxin-responsive transcription factor

  Hirotaka Kato, Haruka Tsugawa, Weijers D., Kimitsune Ishizaki

  International Marchantia Workshop 2024, Nov. 2024, English

  Oral presentation


• R2R3-MYB transcription factors promote regeneration from decapitated explants in the liverwort Marchantia polymorpha

  Gaku Konishi, Yukiko Yasui, Suzuha Tanaka, Yuuki Sakai, Hirotaka Kato, Kimitsune Ishizaki

  International Marchantia Workshop 2024, Nov. 2024, English

  Poster presentation


• 新規機能性野菜としての‘ゼニゴケ’栽培研究

  小山竜平, 北 勇進, 水谷正治, 石崎公庸, 梶川昌孝, 竹村美保, 湯浅正洋, 宇野雄一

  園芸学会令和6年度秋季大会, Nov. 2024, Japanese

  Oral presentation


• 新たな農作物資源としての‘ゼニゴケ’栽培技術の開発

  北 勇進, 水谷 正治, 石崎 公庸, 梶川 昌孝, 竹村 美保, 湯浅 正洋, 宇野 雄一, 小山 竜平

  日本生物環境工学会2024年大阪大会, Sep. 2024, Japanese

  Oral presentation


• ジャスモン酸による維管束幹細胞分化制御機構の解析

  高島 実里, 安井 馨市, 石崎 公庸, 深城 英弘, 伊藤 佑, 古谷 朋之, 近藤 侑貴

  日本植物学会第88回大会, Sep. 2024, Japanese

  Poster presentation


• 二次成長の開始における維管束組織系の幹細胞活性化機構

  島津 舜治, 森 秀世, 古谷 朋之, 小嶋 美紀子, 竹林 裕美子, 伊藤(大橋, 恭子, 石崎 公庸, 朝比奈 雅, 稲垣 宗一, 角谷 徹仁, 深城 英弘, 福田 裕穂, 近藤 侑貴

  日本植物学会第88回大会, Sep. 2024, Japanese

  Poster presentation


• ゼニゴケ葉状体の形態形成におけるROP-GDP解離阻害因子の機能解析

  宮崎 優, 林 愛梨, 酒井 友希, 近藤 侑貴, 深城 英弘, 石崎 公庸

  日本植物学会第88回大会, Sep. 2024, Japanese

  Poster presentation


• 苔類ゼニゴケにおける転写因子WIP過剰発現株の解析

  加藤 大貴, 津川 遥佳, Dolf Weijers, 石崎 公庸

  日本植物学会第88回大会, Sep. 2024, Japanese

  Poster presentation


• 苔類ゼニゴケにおける気室および同化糸の形成異常株の解析

  佐野 あやな, 林 愛梨, 酒井 友希, 石崎 公庸, 佐久間 洋, 加藤 大貴

  日本植物学会第88回大会, Sep. 2024, Japanese

  Poster presentation


• 根とシュートの発生に異常を示すシロイヌナズナ amb2 変異体の解析

  松尾 亮佑, 間宮 章仁, 岩田 健太郎, 村岡 涼子, 近藤 侑貴, 石崎 公庸, 深城 英弘

  日本植物学会第88回大会, Sep. 2024, Japanese

  Oral presentation


• メリステム維持に異常を示すシロイヌナズナ aberrant meristem behavior1 変異体における DNA 損傷応答の解析

  村岡 涼子, 鈴木 洋翔, 間宮 章仁, 近藤 侑貴, 石崎 公庸, 深城 英弘

  日本植物学会第88回大会, Sep. 2024, Japanese

  Oral presentation


• 非維管束植物ゼニゴケのリン吸収における仮根の役割について

  菅野 里美, 福村 日向丸, 喜多 晴哉, 竹平 さくら, 佐 藤 しおり, 酒井 友希, 三村 徹郎, 近藤 侑貴, 深城 英弘, 石崎 公庸

  日本植物学会第88回大会, Sep. 2024, Japanese

  Oral presentation


• ゼニゴケのシトクロム b5 様ヘム結合タンパク質 MpRLF は栄養成長と生殖成長における適切な発生に必要である

  岩田 健太郎, 福村 日向丸, 酒井 友希, 古谷 朋之, 近藤 侑貴, 石崎 公庸, 深城 英弘

  日本植物学会第88回大会, Sep. 2024, Japanese

  Oral presentation


• ゼニゴケの胞子発芽過程におけるROPの機能

  酒井 友希, 石崎 公庸

  日本植物学会第88回大会, Sep. 2024, Japanese

  Oral presentation


• コケ植物における芽の休眠について

  石崎 公庸

  第76回日本細胞生物学会大会, Jul. 2024

  [Invited]

  Nominated symposium


• Elucidation of bisbibenzyl marchantins biosynthesis in Marchantia polymorpha

  Tamao INOUE, Miki MIZUTA, Kimitsune ISHIZAKI, Kojiro TAKANASHI, Masaharu MIZUTANI

  日本農芸化学会2024年度大会, Mar. 2024, Japanese

  Oral presentation


• GCAM1の標的遺伝子GROMはゼニゴケの杯状体形成だけでなく生殖器官の発生も制御する

  加藤大貴, 安居佑季子, 近藤侑貴, 深城英弘, 三村徹郎, 石崎公庸

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 苔類ゼニゴケの細胞膜H+-ATPaseによる形態形成制御の分子機構解析

  水谷未耶, 中根功太郎, 奥村将樹, 楊為雄, 田中周, 井上普一郎, 藤原徹, 神谷岳洋, 反田直之, 西浜竜一, 石崎公庸, 木下俊則

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• ゼニゴケのシトクロムb5様ヘム結合タンパク質MpRLFは栄養成長と生殖成長における適切な発生に必要である

  岩田健太郎, 福村日向丸, 酒井友希, 古谷朋之, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• コケ植物ゼニゴケのリン吸収における仮根の機能について

  菅野里美, 福村日向丸, 喜多晴哉, 佐藤しおり, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 経時的遺伝子発現変化から理解する苔類ゼニゴケの胞子発芽過程

  酒井友希, 島津舜治, 近藤侑貴, 深城英弘, 石崎公庸

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 維管束細胞分化を制御する糖シグナル伝達機構の解析

  吉田善葵, 鳴瀧葵, 島津舜治, 石崎公庸, 深城英弘, 近藤侑貴

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• 根系パターンに異常を示すシロイヌナズナbird feather変異体の解析

  伊佐積希, 岩田健太郎, 間宮章仁, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• 根の形態形成に異常を示す新たなシロイヌナズナ変異体の探索と解析

  小栗聡太, 岩田健太郎, 間宮章仁, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• ゼニゴケにおけるMarchantin類生合成経路遺伝子の探索

  水田珠希, 井上珠緒, 石崎公庸, 高梨功次郎, 水谷正治

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• bHLH型転写因子MpHYPNOSはゼニゴケ無性芽の休眠を制御する

  芳村那美, ��川実樺子, 安田有沙, 加藤大貴, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎, 公庸

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• ゼニゴケ雌雄株間での塩ストレス耐性の違いを決定する量的形質遺伝子座（QTLs）の探索

  熊野椋介, 石崎公庸, 松村英生, 堀江智明

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• N6-メチルアデノシン（m6A）は植物のDNA損傷修復に必要か？

  松尾亮佑, 間宮章仁, 岩田健太郎, 村岡涼子, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• 維管束細胞の分化制御における概日時計関連遺伝子GIの機能解析

  阪田真由, 荒野巧勝, 島津舜治, 深城英弘, 石崎公庸, 近藤侑貴

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• スクロースシグナリングは細胞分化を阻害することで維管束形成層の維持にはたらく

  鳴瀧葵, 島津舜治, 古谷朋之, 深城英弘, 石崎公庸, 近藤侑貴

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• 非維管束植物ゼニゴケにおける液胞膜リン酸輸送体VPTの機能解析

  林田雅大, 佐藤しおり, 福村日向丸, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

  第65回日本植物生理学会年会（神戸）, Mar. 2024, English

  Poster presentation


• シロイヌナズナSUR2/CYP83B1遺伝子の変異アリルfsp1は側根創始細胞形成の側方抑制に働くTOLS2ペプチドに対して低感受性を示す

  後藤千恵子, 前原菜々子, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 維管束幹細胞の確立に関する新たな制御因子の探索と解析

  前田照太, 島津舜治, 間宮章仁, 古谷朋之, 深城英弘, 石崎公庸, 近藤侑貴

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 側根形成に異常を示すシロイヌナズナlbd多重変異体を用いたカルス形成・シュート再生機構の解析

  和田昂己, 間宮章仁, 郷達明, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• メリステム維持に異常を示すシロイヌナズナaberrant meristem behavior1変異体におけるDNA損傷応答の解析

  村岡涼子, 間宮章仁, 近藤侑貴, 石崎公庸, 深城英弘

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 陸上植物におけるprospindleの形成機構と役割について

  佐々木武馬, 石崎公庸, 本瀬宏康, 小田祥久

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• 一過的サイトカイニン応答は二次成長開始のプライミングシグナルとして機能する

  島津舜治, 森秀世, 古谷朋之, 小嶋美紀子, 竹林裕美子, 伊藤(大橋)恭子, 石崎公庸, 朝比奈雅志, 榊原均, 稲垣宗一, 角谷徹仁, 深城英弘, 福田裕穂, 近藤侑貴

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• ゼニゴケにおける硝酸応答機構の解析

  大濱直彦, 石崎公庸, 林誠, 柳澤修一

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• ゼニゴケCHLH遺伝子の変異はPPO阻害除草剤耐性を付与する

  北岡理杜, 小牧知史, 酒井友希, 加藤大貴, 近藤侑貴, 深城英弘, 石崎公庸

  第65回日本植物生理学会年会（神戸）, Mar. 2024, Japanese

  Oral presentation


• MpHYPNOS regulates gemma dormancy in Marchantia polymorpha

  Nami Yoshimura, Mikako Yoshikawa, Arisa Yasuda, Hirotaka Kato, Yuuki Sakai, Tetsuro, Mimura4Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

  Taiwan-Japan Plant Biology 2023 (Taipei, Taiwan), Oct. 2023, English

  Poster presentation


• ゼニゴケの実用化に向けて ー食用ゼニゴケと合成生物学プラットフォームー

  水谷正治, 小山竜平, 梶川昌孝, 竹村美保, 石崎公庸

  第40回日本植物バイオテクノロジー学会, Sep. 2023, Japanese

  [Invited]

  Nominated symposium


• 陸上植物においてオーキシンの下流で働く転写因子WIPの機能解析

  加藤大貴, Weijers Dolf, 石 崎公庸

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• ゼニゴケの器官形成を制御するROPシグナリング

  酒井友希, 米塚広樹, 上野亜 紀, 加藤大貴, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• 植物の器官発生におけるシトクロムb5様ヘム結合タンパク質RLFの機能解析

  岩田健太郎, 後藤千恵子, 福 村日向丸, 清水隆之, 丸山海 成, 古谷朋之, 近藤侑貴, 笠原博幸, 増田建, 石崎公 庸, 深城英弘

  Sep. 2023, Japanese

  Oral presentation


• サイトカイニンは形成層幹細胞の活性化シグナルとして機能する

  島津舜治, 森秀世, Alif Meem Nurani, 山田一貴, 柴田 恭美, 古谷朋之, 伊藤(大橋) 恭子, 石崎公庸, 深城英弘, 朝 比奈雅志, 稲垣宗一, 角谷徹 仁, 福田裕穂, 近藤侑貴

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• 側根形成に異常を示すシロイ ヌナズナlbd多重変異体を用いたカルス形成・シュート再生機構の解析

  和田昂己, 間宮章仁, 郷達明, 近藤侑貴, 石崎公庸, 深城英弘

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• 陸上植物における紡錘体軸および細胞分裂方向の制御機構

  佐々木武馬, 石崎公庸, 本瀬 宏康, 小田祥久

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• 維管束細胞の分化制御における概日時計関連遺伝子GIの機能解析

  阪田真由, 荒野巧勝, 島津舜 治, 深城英弘, 石崎公庸, 近藤侑貴

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• スクロースシグナルによる維管束幹細胞制御機構の解析

  鳴瀧葵, 島津舜治, 古谷朋 之, 深城英弘, 石崎公庸, 近 藤侑貴

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• 維管束幹細胞の確立に関する新たな制御因子の探索と分子機構の解析

  前田照太, 島津舜治, 古谷 朋之, 深城英弘, 石崎公庸, 近藤侑貴

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• ゼニゴケCHLH遺伝子の変異はPPO阻害除草剤耐性を付与する

  北岡理杜, 小牧知史, 酒井友 希, 加藤大貴, 近藤侑貴, 深城英弘, 石崎公庸

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• MpHYPNOSはABA依存的および非依存的に無性芽の休眠を制御する

  芳村那美, ����川実樺子, 安田 有沙, 加藤大貴, 酒井友 希, 三村徹郎, 近藤侑貴, 深 城英弘, 石崎公庸

  日本植物学会第87回大会（北海道大学工学部）, Sep. 2023, Japanese

  Oral presentation


• 根系パターンに異常を示すシロイヌナズナbird feather変異体の解析

  伊佐 積希, 岩田 健太郎, 間宮 章仁, 近藤 侑貴, 石崎 公 庸, 深城 英弘

  日本植物学会第87回大会（北海道大学−オンライン）, Sep. 2023, Japanese

  Poster presentation


• 根の形態形成に異常を示す新たなシロイヌナズナ変異体の探索と解析

  小栗 聡太, 岩田 健太郎, 間宮 章仁, 近藤 侑貴, 石崎 公 庸, 深城 英弘

  日本植物学会第87回大会（北海道大学−オンライン）, Sep. 2023, Japanese

  Poster presentation


• 非維管束植物ゼニゴケにおける液胞膜リン酸輸送体VPTの機能解析

  林田 雅大, 佐藤 しおり, 酒井 友希, 三村 徹郎, 近藤 侑貴, 深城 英弘, 石崎 公庸

  日本植物学会第87回大会（北海道大学−オンライン）, Sep. 2023, Japanese

  Poster presentation


• Functional analysis of RLF, a cytochrome b5-like heme binding protein, in plant organ development

  Kentaro Iwata, Chieko Goto, Hinatamaru Fukumura, Takayuki Shimizu, Kaisei Maruyama, Tomoyuki Furuya, Yuki Kondo, Hiroyuki Kasahara, Tatsuru Masuda, Kimitsune Ishizaki, Hidehiro Fukaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• Leaf position and season-dependent transcriptome analysis of field-grown poplar cuttings

  Yuko Kurita, Makoto Kashima, Kei’ichi Baba, Kimitsune Ishizaki, Natsuko Kobayashi, Keitaro Tanoi, Tetsuro Mimura, Atsushi J. Nagano

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• Role of ROP signaling in the growth and organogenesis of Marchantia polymorpha

  Yuuki Sakai, Hiroki Yonetsuka, Aki Ueno, Hirotaka Kato, Tetsuro Mimura, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• MpBZR3 regulates gametangium development in Marchantia polymorpha

  Tomoyuki Furuya, Natsumi Saegusa, Shohei Yamaoka, Chiaki Yamamoto, Shunji Simadzu, Naoki Minamino, Ryuichi Nishihama, Kimitsune Ishizaki, Takashi Ueda, Hidehiro Fukaki, Takayuki Kohchi, Masahiro Kasahara, Hiroo Fukuda, Takashi Araki, Yuki Kondo

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• Mechanisms of MpHYPNOS-mediated gemma dormancy in Marchantia polymorpha

  Nami Yoshimura, Mikako Yoshikawa, Arisa Yasuda, Hirotaka Kato, Yuuki Sakai, Tetsuro Mimura, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• Functional analysis of Non-specific phospholipase C in Marchantia polymorpha

  Daisuke Uchikoshi, Misao Shimojo, Koichi Hori, Kimitsune Ishizaki, Hiroyuki Ohta, Mie Shimojima

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• The role of MYBCC-SPX module in phosphate response of nonvascular plant Marchantia polymorpha

  Hinatamaru Fukumura, Ginga Kitaura, Hirotaka Kato, Yuuki Sakai, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Oral presentation


• Analysis of DNA damage response in a novel Arabidopsis mutant showing aberrant root and shoot development

  Ryoko Muraoka, Akihito Mamiya, Yuki Kondo, Kimitsune Ishizaki, Hidehiro Fukaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Poster presentation


• Establishment of a vascular cell induction system using Ginkgo biloba leaves

  Keishi Yasui, Shunji Shimadzu, Aoi Narutaki, Shota Maeda, Kimitsune Ishizaki, Hidehiro Fukaki, Yuki Kondo

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Poster presentation


• The single MYB gene GROM is required for gemma cup formation of the liverwort Marchantia polymorpha as a direct target of GCAM1

  Hirotaka Kato, Yukiko Yasui, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Poster presentation


• SUR2 Fine-tunes Local Auxin Distribution for Lateral Root Formation in Arabidopsis thaliana

  Chieko Goto, Akira Ikegami, Tatsuaki Goh, Hiroyuki Kasahara, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Poster presentation


• Analysis of oligogalactolipid synthesis and function in Marchantia polymorpha

  Shinsuke Shimizu, Koichi Hori, Kimitsune Ishizaki, Mie Shimojima, Hiroyuki Ohta

  The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Mar. 2023, English

  Poster presentation


• MpHYPNOSを介したゼニゴケ無性芽の休眠制御機構

  芳村 那美, 石崎 公庸

  第5回 コケ幹細胞研究会（服部植物研究所）, Dec. 2022, Japanese

  Oral presentation


• ゼニゴケ胞子における不等分裂メカニズム

  酒井 友希, 石崎 公庸

  第5回 コケ幹細胞研究会（服部植物研究所）, Dec. 2022, Japanese

  Oral presentation


• ゼニゴケにおける栄養繁殖の分子機構

  石崎 公庸

  第5回 コケ幹細胞研究会（服部植物研究所）, Dec. 2022, Japanese

  Oral presentation


• G protein-signaling and metabolic pathways as evolutionarily conserved mechanisms to combat calcium deficiency

  Richalynn Leong, Javier Tan, Sally Koh, Wu Ting-Ying, Kimitsune Ishizaki, Daisuke Urano

  EMBO workshop on "An Integrated View of Early Land Plant Evolution", Nov. 2022, English

  Poster presentation


• Dormancy of vegetative propagules in a liverwort Marchantia polymorpha

  Kimitsune Ishizaki

  EMBO workshop on "An Integrated View of Early Land Plant Evolution", Nov. 2022, English

  [Invited]

  Nominated symposium


• 根とシュートの成長・発生に異常を示すシロイヌ ナズナamb変異体におけるDNA損傷応答の解析

  村岡 涼子, 間宮 章仁, 近藤 侑貴, 石崎 公庸, 深城 英弘

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• ゼニゴケのヘム結合タンパク質MpRLFは適切な葉状体と杯状体の形成に必要である

  岩田 健太郎, 福村 日向丸, 近藤 侑貴, 石崎 公庸, 深城 英弘

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• 陸上植物におけるガス交換系の発生と環境への適応

  水谷 未耶, 内橋 貴之, Christian Ganser, 林 優紀, 大川 諒也, 大西(小川) 真理, 松林 嘉克, 西浜 竜一, 木下 俊則, 石崎 公庸, 河内 孝之, 東山 哲也, 金岡 雅浩

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• ゼニゴケ無性芽の休眠を促進するMpHYPNOSの機能

  芳村 那美, ��川実樺子, 安田 有沙, 加藤 大貴, 酒井 友希, 三村 徹郎, 近藤 侑貴, 深城 英弘, 石崎 公庸

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• ゼニゴケ配偶子器の発生におけるMpBZR3の役割

  古谷 朋之, 三枝 菜摘, 山岡, 尚平, 島津 舜治, 山本 千愛, 石崎 公庸, 西浜 竜一, 河内 孝之, 福田 裕穂, 笠原 賢洋, 荒木 崇, 近藤 侑貴

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• 落葉木本植物ポプラにおける葉位ごとの季節的なシンク-ソース推移の解明

  栗田 悠子, 鹿島 誠, 馬場 啓一, 小林 奈通子, 田野井 慶太朗, 石崎 公庸, 三村 徹郎, 永野 惇

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• コケ植物ゼニゴケの脅威の繁殖力を支える分子基盤

  酒井 友希, 加藤 大貴, 安居 佑季子, 石崎 公庸

  日本植物学会第86回大会, Sep. 2022, Japanese

  [Invited]

  Nominated symposium


• 薬用植物ニチニチソウの種子発芽過程におけるアルカロイド代謝開始過程の解析

  鵜崎 真妃, 山本 浩太郎, 森 哲也, 村上 明男, 大西 美輪, 七條 千津子, 石崎 公庸, 深城 英弘, 三村 徹郎, 平井 優美

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• 維管束幹細胞の分裂と分化を制御するサイトカイニンの機能と動態

  島津 舜治, Nurani Alif Meem, 森 秀世, 山田 一貴, 柴田 恭美, 古谷 朋之, 伊藤(大橋) 恭子, 石崎 公庸, 深城 英弘, 朝比奈 雅志, 稲垣 宗一, 角谷 徹仁, 福田 裕穂, 近藤 侑貴

  日本植物学会第86回大会, Sep. 2022, Japanese

  Oral presentation


• 糖シグナルによる維管束幹細胞制御機構の解析

  鳴瀧 葵, 島津 舜治, 古谷 朋之, 深城 英弘, 石崎 公庸, 近藤 侑貴

  日本植物学会第86回大会, Sep. 2022, Japanese

  Poster presentation


• シロイヌナズナ側根形成変異体を用いたカルス形成機構の解析

  和田 昂己, 間宮 章仁, 近藤 侑貴, 石崎 公庸, 深城 英弘

  日本植物学会第86回大会, Sep. 2022, Japanese

  Poster presentation


• PPO 阻害除草剤耐性ゼニゴケの単離と解析

  北岡 理杜, 小牧 知史, 酒井 友希, 加藤 大貴, 近藤 侑貴, 深城 英弘, 石崎 公庸

  日本植物学会第86回大会, Sep. 2022, Japanese

  Poster presentation


• コケ植物ゼニゴケのリン欠乏応答を制御するMYBCC 型転写因子の機能解析

  福村 日向丸, 北浦 銀河, 加藤 大貴, 酒井 友希, 近藤 侑貴, 深城 英弘, 三村 徹郎, 石崎 公庸

  日本植物学会第86回大会, Sep. 2022, Japanese

  Poster presentation


• The role of MYBCC-SPX module in phosphate starvation of nonvascular plant Marchantia polymorpha

  Hinatamaru Fukumura, Ginga Kitaura, Hirotaka Kato, Yuuki Sakai, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Functional analysis of WANDERING ROOT1 and WAD2 genes, rice DECUSSATE homologues, in Arabidopsis root system construction

  Ikumi Azuma, Risa Kimura, Ken-Ichiro Hibara, Tatsuaki Goh, Akihito Mamiya, Tetsuro Mimura, Yuki Kondo, Kimitsune Ishizaki, Jun-Ichi Itoh, Hidehiro Fukaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Functional analysis of MpBZR3 on the regulation of gametangia development in Marchantia polymorpha

  Tomoyuki Furuya, Shohei Yamaoka, Kimitsune Ishizaki, Ryuichi Nishihama, Takashi Araki, Takayuki Kohchi, Hiroo Fukuda, Yuki Kondo

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Analysis of the regulatory mechanisms of root growth and development via the K+ efflux channel GORK

  Daisuke Ide, Riku Nishimaru, Yuka Aoki, Koichi Toyokura, Tatsuaki Goh, Tetsuro Mimura, Yuki Kondo, Kimitsune Ishizaki, Hidehiro Fukaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Analysis of the Arabidopsis mutants showing altered response to the TOLS2 peptide, an inhibitor of lateral root formation

  Nanako Maehara, Akihito Mamiya, Chieko Goto, Tatsuaki Goh, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Analysis of a novel Arabidopsis mutant showing abnormalities in root and shoot development

  Ryoko Muraoka, Yuki Kondo, Kimitsune Ishizaki, Hidehiro Fukaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Asymmetric division in the spore of Marchantia polymorpha

  Yuuki Sakai, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• Initiation process of monoterpenoid indole alkaloid biosynthesis during seed germination in Catharanthus roseus

  Mai Uzaki, Kotaro Yamamoto, Amit Rai, Akio Murakami, Miwa Ohnishi, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Sarah O’Connor, Tetsuro Mimura, Masami, Yokota Hirai

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Poster presentation


• The single MYB gene GROM regulates gemma cup formation of the liverwort Marchantia polymorpha

  Hirotaka Kato, Yukiko Yasui, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• Functional analysis of RLF, a cytochrome b5-like heme binding protein, in lateral root formation

  Kentaro Iwata, Chieko Goto, Kaisei Maruyama, Hinatamaru Fukumura, Yuki Kondo, Kimitsune Ishizaki, Hiroyuki Kasahara, Hidehiro Fukaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• The fsp1/sur2 mutation that increases the auxin content restores lateral root formation in the fwr/gnom mutant of Arabidopsis thaliana

  Chieko Goto, Akira Ikegami, Tatsuaki Goh, Hiroyuki Kasahara, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• Functional analysis of cytokinin in cell fate regulation of vascular stem cells

  Shunji Shimadzu, Alif Meem Nurani, Kazuki Yamada, Kyomi Shibata, Tomoyuki Furuya, Kyoko Ohashi-Ito, Kimitsune Ishizaki, Hidehiro Fukaki, Masashi Asahina, Hiroo Fukuda, Yuki Kondo

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• Role of the microtubule-associated protein CORD in Marchantia

  Takema Sasaki, Kimitsune Ishizaki, Hiroyasu Motose, Yoshihisa Oda

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• MpHYPNOS-mediated regulatory mechanism of gemma dormancy in Marchantia polymorpha

  Nami Yoshimura, Mikako Yoshikawa, Arisa Yasuda, Hirotaka Kato, Yuuki Sakai, Tetsuro Mimura, Yuki Kondo, Hidehiro Fukaki, Kimitsune Ishizaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• Marchantia DWF5A which is responsible for sterol 7-position reduction reaction, is involved in the development of thallus

  Miki Hatada, Ryota Akiyama, Kimitsune Ishizaki, Masaharu Mizutani

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  Oral presentation


• Evolutionary conserved mechanisms of stem cell proliferation in land plants

  Kimitsune Ishizaki

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, English

  [Invited]

  Invited oral presentation


• The analysis of the function of GPAT genes on surface lipid synthesis in Marchantia polymorpha

  Motoki Fuki, Koichi Hori, Yuta Ihara, Kimitsune Ishizaki, Mie Shimojima, Hiroyuki Ohta

  第63回 日本植物生理学会年会（つくば：オンライン）, Mar. 2022, Japanese

  Oral presentation


• ³²P及び³³Pを用いた樹木の季節的なリン転流経路の可視化

  栗田 悠子, 菅野 里美, 杉田 亮平, 廣瀬 農, 大西 美輪, 石崎 公庸, 深城 英弘, 馬場 啓一, 永野 惇, 三村 徹郎, 中西 友子, 小林 奈通子, 田野井 慶太朗

  アイソトープ・放射線研究発表会, 2022, Japanese, 公益社団法人 日本アイソトープ協会


• 基部陸上植物ゼニゴケにおけるリンの吸収・分配・貯蔵のメカニズム

  佐藤しおり, 福村日向丸, 加藤大貴, 酒井友希, 近藤侑貴, 深城英弘, 三村徹郎, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Oral presentation


• ゼニゴケ配偶子器の発生を制御する非典型BZR 転写因子

  古谷朋之, 山岡尚平, 石崎公庸, 西浜竜一, 荒木崇, 河内孝之, 福田裕穂, 近藤侑貴

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Oral presentation


• 陸上植物に保存されたオーキシン応答遺伝子WIP のゼニゴケにおける機能解析

  加藤大貴, 近藤侑貴, 深城英弘, Dolf Weijers, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Oral presentation


• 発光レポーター遺伝子を用いた側根プレパターニング変異体の単離と解析

  小笹綾香, 後藤千恵子, 郷達明, 近藤侑貴, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021


• 側根形成能が顕著に低下するシロイヌナズナ変異体fwr とその抑圧変異体fsp1 の解析

  後藤千恵子, 池上聡, 郷達明, 笠原博幸, 近藤侑貴, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Oral presentation


• 位置情報による維管束幹細胞の運命制御

  島津舜治, Alif Meem Nurani, 山田一貴, 柴田恭美, 古谷朋之, 伊藤(大橋)恭子, 石崎公庸, 深城英弘, 朝比奈雅志, 福田裕穂, 近藤侑貴

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Oral presentation


• ゼニゴケ器官形成におけるR2R3-MYB 転写因子SHOTGLASS の機能

  酒井友希, 高見英幸, 山岡尚平, 近藤侑貴, 深城英弘, 三村徹郎, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• カリウムイオン排出チャネルGORK を介した根の成長・発生制御機構の解析

  井手大輔, 西丸陸, 青木優佳, 豊倉浩一, 郷達明, 近藤侑貴, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• ゼニゴケ葉状体の形態形成におけるMpREN の機能解析

  米塚広樹, 上野亜紀, 樋渡琢真, 加藤大貴, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• シロイヌナズナ側根形成を抑制するTOLS2 ペプチドに対する応答異常変異体の探索と解析

  前原菜々子, 間宮章仁, 後藤千恵子, 郷達明, 近藤侑貴, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• シロイヌナズナ側根形成を抑制するTOLS2 ペプチド低感受性変異体toti1 の解析

  間宮章仁, 雨川智美, 豊倉浩一, 青木優佳, 郷達明, 近藤侑貴, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• 側根形成におけるシトクロムb5 様ヘム/ ステロイド結合タンパク質RLF の機能解析

  岩田健太郎, 後藤千恵子, 福村日向丸, 近藤侑貴, 石崎公庸, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese


• 根系構築に異常を示すシロイヌナズナ新規変異体の解析

  村岡涼子, 近藤侑貴, 石﨑公庸, 深城英弘

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• ゼニゴケの葉状体再生におけるGCAM1 およびGC1L の機能

  田中涼葉, 加藤大貴, 安居佑季子, 近藤侑貴, 深城英弘, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• 基部陸上植物ゼニゴケのリン欠乏応答におけるMpMYBCC-MpSPX モジュールの機能

  福村日向丸, 北浦銀河, 加藤大貴, 酒井友希, 深城英弘, 近藤侑貴, 三村徹郎, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• ゼニゴケ無性芽におけるMpHYPNOS を介した休眠制御

  芳村那美, 吉川実樺子, 安田有沙, 加藤大貴, 酒井友希, 三村徹郎, 近藤侑貴, 深城英弘, 石崎公庸

  日本植物学会 第85回大会（八王子：オンライン）, Sep. 2021, Japanese

  Poster presentation


• Isolation and analysis of lateral root pre-patterning mutants using the luminescence reporter gene

  Ayaka Ozasa, Chieko Goto, Tatsuaki Goh, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Poster presentation


• Analysis of fewer roots suppressor 1 (fsp1) in which the mutation suppresses the fewer roots (fwr) phenotype for lateral root formation

  Chieko Goto, Akira Ikegami, Tatsuaki Goh, Hiroyuki Kasahara, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Oral presentation


• Cell fate regulation of vascular stem cell via cytokinin signaling

  Shunji Shimadzu, Tomoyuki Furuya, Kyoko Ohashi-Ito, Kimitsune Ishizaki, Hidehiro Fukaki, Hiroo Fukuda, Yuki Kondo

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Oral presentation


• Analysis of changes in alkaloid metabolism during germination in Catharanthus roseus

  Mai Uzaki, Kotaro Yamamoto, Akio Murakami, Miwa Ohnishi, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura, Masami Yokota Hirai

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Oral presentation


• Functional analysis of a R2R3-MYB transcription factor SHOTGLASS in Marchantia polymorpha

  Yuuki Sakai, Hideyuki Takami, Shigeyuki Tsukamoto, Shohei Yamaoka, Yuki Kondo, Hidehiro Fukaki, Tetsuro Mimura, Kimitsune Ishizaki

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Oral presentation


• Functional characterization of LAX PANICLE2 homologous in the liverwort Marchantia polymorpha

  Naho Maehara, Hirotaka Kato, Yuki Sakai, Yuki Kondo, Tetsuro Mimura, Hidehiro Fukaki, Kimitsune Ishizaki

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Oral presentation


• The roles of plant specific BZR transcription factors in Marchantia polymorpha

  Tomoyuki Furuya, Shohei Yamaoka, Kimitsune Ishizaki, Ryuichi Nishihama, Takashi Araki, Takayuki Kohchi, Hiroo Fukuda, Yuki Kondo

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  Oral presentation


• Exploring the common mechanisms for stem cell propagation from vegetative reproduction of Marchantia polymorpha

  Hirotaka Kato, Yukiko Yasui, Kimitsune Ishizaki

  第62回日本植物生理学会年会（松江：オンライン開催）, Mar. 2021, Japanese

  [Invited]

  Nominated symposium


• 無性芽形成に重要なKAR/RopGEFを制御する上流因子の探索

  樋渡琢真, 南野尚紀, 海老根一生, 深城英弘, 三村徹郎, 上田貴志, 石崎公庸

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Poster presentation


• 薬用植物ニチニチソウをモデルとしたアルカロイド代謝分化過程の解析

  鵜崎真妃, 山本浩太郎, 村上明男, 大西美輪, 七條千津子, 高橋勝利, 石崎公庸, 深城英弘, 三村徹郎, 平井優美

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Poster presentation


• ポプラ短縮周年系を用いた季節的なリン転流機構の解明と野外RNA-seqとの比較

  栗田悠子, 馬場啓一, 手塚あゆみ, 出口亜由美, 大西美輪, 石崎公庸, 深城英弘, 三村徹郎, 永野惇

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Oral presentation


• ゼニゴケ仮根の先端成長におけるリン酸化とCa2+結合を介したROS生成酵素の活性制御機構

  橋本研志, 阿部尚明, 板橋武, 石崎公庸, 西浜竜一, 河内孝之, 朽津和幸

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Oral presentation


• ゼニゴケ側生器官形成を制御するMYB転写因子GCAM2の機能解析

  酒井友希, 高見英幸, 塚本成幸, 山岡尚平, 増田晃秀, 深城英弘, 三村徹郎, 河内孝之, 石崎公庸

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Oral presentation


• 苔類ゼニゴケの杯状体形成に重要なsingle-MYBタンパク質の同定

  加藤大貴, 安居佑季子, 深城英弘, 三村徹郎, 石崎公庸

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Oral presentation


• 基部陸上植物ゼニゴケにおけるリン酸欠乏に応答した遺伝子転写制御

  北浦銀河, 佐藤しおり, 加藤大貴, 深城英弘, 三村徹郎, 石崎公庸

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  Oral presentation


• 食虫植物の栄養認識機構の解析

  三村徹郎, 市川紗季, 谷口裕志, 公手晃太郎, 安井悦子, 篠田亜貴子, 石崎公庸, 深城英弘, 深城英弘, 大西美輪

  第84回日本植物学会大会（名古屋：オンライン開催）, Sep. 2020, Japanese

  [Invited]

  Public symposium


• Marchantia polymorpha as a model for evolutionary biology

  ISHIZAKI Kimitsune

  The Vassilios Sarafis OzBryo Meeting, Dec. 2019, English, Melbourne, Australia, International conference

  [Invited]

  Invited oral presentation


• An evolutionary conserved mechanism for production of secondary meristems in land plants

  ISHIZAKI Kimitsune

  Australian Society of Plant Scientists Conference: ASPS 2019, Nov. 2019, English, Melbourne, Australia, International conference

  [Invited]

  Invited oral presentation


• GEMMA CUP-ASSOCIATED MYB1, an orthologue of axillary meristem regulator, is essential for vegetative reproduction in the liverwort Marchantia polymorpha

  ISHIZAKI Kimitsune

  Marchantia Workshop 2019, Sep. 2019, English, Tohoku University, Sendai, Japan, Japan, International conference

  [Invited]

  Oral presentation


• 重イオンビーム照射胞子を用いたゼニゴケ配偶体形態形成変異体のスクリーニングと解析

  酒井嵩人, 平田千穂, 風間裕介, 阿部知子, 三村徹郎, 深城英弘, 石崎公庸

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• レポーター遺伝子を用いたシロイヌナズナ側根プレパターニング機構の解析

  小笹綾香, 郷達明, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• シロイヌナズナ側根形成能が顕著に低下するfewer roots変異体のサプレッサー変異体の解析

  田井中芳樹, 郷達明, 石崎公庸, 三村徹郎, 深城英弘

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• ゼニゴケ形態形成におけるRopGAPの機能解析

  上野亜紀, 樋渡琢真, 三村徹郎, 深城英弘, 石崎公庸

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• ニチニチソウ乳管細胞・異形細胞の発生，分化，代謝変動の解析

  鵜崎真妃, 山本浩太郎, 高橋勝利, 大西美輪, 栗田悠子, 七條千津子, 永野惇, 石崎公庸, 深城英弘, 三村徹郎

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケにおけるリン応答と転流の解析

  佐藤しおり, 北浦銀河, 三村徹郎, 深城英弘, 石崎公庸

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• ゼニゴケ組織分化へのBZR 転写因子ファミリーの寄与

  古谷朋之, 石崎公庸, 西浜竜一, 河内孝之, 福田裕穂, 近藤侑貴

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Oral presentation


• ゼニゴケ無性芽形成に機能するRopおよびRopGEFの細胞内局在解析

  樋渡琢真, 上野亜紀, 金澤建彦, 南野尚紀, 深城英弘, 三村徹郎, 上田貴志, 石崎公庸

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• ゼニゴケ頂端分裂組織の細胞分裂・分化制御におけるROS生成酵素MpRbohの役割

  萩原雄樹, 宮本大輔, 髙川智弘, 橋本研志, 西浜竜一, 石崎公庸, 河内孝之, 朽津和幸

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Poster presentation


• 根系の光環境が植物のリン酸応答に与える影響

  吉岡優介, 大西美輪, 栗田悠子, 木下俊則, 永野淳, 石崎公庸, 深城英弘, 三村徹郎

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Oral presentation


• 生育土壌の違いによる植物体イオノームの変動―植物体から生 育場所を推定できるか？―

  天野百々江, 大西美輪, 石崎公庸, 深城英弘, 三村徹郎

  日本植物学会第83回大会, Sep. 2019, Japanese

  [Invited]

  Oral presentation


• E3リガーゼNOPPERABO1は細胞壁の再構築を介して陸上植物 の通気組織形成を制御する

  水谷未耶, 林優紀, Christian Ganser, 内橋貴之, 石崎公庸, 西浜竜一, 木下俊則, 河内孝之, 東山哲也, 金岡雅浩

  日本植物学会第83回大会, Sep. 2019, Japanese

  Oral presentation


• TOLS2ペプチドによる側根形成関連遺伝子の発現制御に異常を 示す変異体の解析

  西丸 陸, 青木 優佳, 豊倉 浩一, 篠 田 明徳, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  日本植物学会第83回大会, Sep. 2019, Japanese, 仙台, Japan, Domestic conference

  Oral presentation


• An evolutionarily conserved mechanism for production of secondary meristems in land plants

  ISHIZAKI KIMITSUNE

  International Symposium: Principles of pluripotent stem cells underlying plant vitality, May 2019, English, Auditorium of Graduate School of Life Sciences, Katahira Campus, Tohoku University, Japan, Vegetative reproduction, a form of asexual reproduction in plants, is a developmental process by which clonal progeny arise directly from parental tissues. This process is based on the remarkable potential of plants to proliferate meristems, which develop whole plantlets from differentiated tissues. A basal land plant, the liverwort Marchantia polymorpha L., as well as certain, International conference

  [Invited]

  Invited oral presentation


• 陸上植物に共通するガス交換組織形成機構の理解

  水谷未耶, 林優紀, 石崎公庸, 西浜竜一, 木下俊則, 河内孝之, 東山哲也, 金岡雅浩

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• 転写因子D53 はゼニゴケ杯状体に形成される無性芽数の決定に関わる

  小松愛乃, 水野陽平, 児玉恭一, 島崎翔太, 楢本悟史, 石崎公庸, 経塚淳子

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• ニチニチソウ乳管細胞・異形細胞の発生、分化、代謝変動の解析

  鵜崎真妃, 山本浩太郎, 高橋勝利, 大西美輪, 栗田悠子, 七條千津子, 永野惇, 石崎公庸, 深城英弘, 三村徹郎

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• セントポーリアの温度降下感受性に関わる分子機構の解析

  本岡香奈, 大西美輪, 飯田和子, 鈴木祥弘, 石崎公庸, 深城英弘, 飯田秀利, 三村徹郎

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Poster presentation


• ゼニゴケ腹鱗片の粘液細胞形成におけるRop シグナル伝達の役割

  樋渡琢真, 深城英弘, 三村徹郎, 石崎公庸

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Poster presentation


• ゼニゴケの表層脂質合成系の解析

  高橋佑弥, 堀孝一, 石崎公庸, 下嶋美恵, 太田啓之

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• ゼニゴケの頂端分裂組織周辺におけるROS 生成酵素MpRbohA の役割の解析

  萩原雄樹, 宮本大輔, 髙川智弘, 橋本研志, 西浜竜一, 石崎公庸, 河内孝之, 朽津和幸

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• ゼニゴケの2 つのR2R3-MYB 型転写因子を介した異なる幹細胞性獲得制御機構

  安居佑季子, 塚本成幸, 石田咲子, 西浜竜一, 深城英弘, 三村徹郎, 河内孝之, 石崎公庸

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• ゼニゴケにおけるMpCLE2 の内在的な機能の解析

  平川有宇樹, 藤本童子, 打田直行, 澤進一郎, 石崎公庸, 西浜竜一, 河内孝之, 清末知宏, ボウマンジョン

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• ゼニゴケにおけるEndoglucanase16 の機能解析

  石田光南, 黒羽剛, 石崎公庸, 檜垣匠, 楢本悟史, 横山隆亮, 小竹敬久, 西谷和彦

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• シロイヌナズナ側根形成を抑制するTOLS2 ペプチドによる遺伝子の発現制御に異常を示す変異体の解析

  西丸陸, 青木優佳, 豊倉浩一, 篠田明徳, 郷達明, 石崎公庸, 三村徹郎, 深城英弘

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Poster presentation


• GSK3-BES1 シグナリングモジュールのゼニゴケにおける役割

  古谷朋之, 石崎公庸, 西浜竜一, 河内孝之, 福田裕穂, 近藤侑貴

  第60回日本植物生理学会年会, Mar. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• ゼニゴケにおけるMpCLE2の内在的な機能の解析

  平川有宇樹, 藤本童子, 打田直行, 澤進一郎, 石崎公庸, 西浜竜一, 河内孝之, 清末知宏, BOWMAN John

  日本植物生理学会年会(Web), 2019, Japanese


• ゼニゴケの頂端分裂組織周辺におけるROS生成酵素MpRbohAの役割の解析

  萩原雄樹, 宮本大輔, 高川智弘, 橋本研志, 西浜竜一, 石崎公庸, 河内孝之, 朽津和幸, 朽津和幸

  日本植物生理学会年会(Web), 2019, Japanese


• Molecular genetics of gemma dormancy in a basal land plantMarchantia polymorpha

  ISHIZAKI KIMITSUNE, Shigeyuki Tsukamoto, FUKAKI HIDEHIRO, MIMURA TETSURO, Daisuke Takezawa, Yoichi Sakata, Takayuki Kohchi

  6th Plant Dormancy Symposium, Oct. 2018, English, Kyoto, Japan, Dormancy is a critical strategy of plants to survive under severe environmental conditions by pausing growth, development, and physical activity, which can occur in diverse organs such as seeds and buds of diverse plant lineages. The liverwort Marchantia polymorpha, reproduces asexually by clonal individuals, gemmae, that are dormant within the specialized receptacle, called ge, International conference

  [Invited]

  Invited oral presentation


• 陸上植物の生殖細胞分化に必要な転写因子BONOBOの同定と標的遺伝子の探索

  山岡 尚平, 西浜 竜一, 吉竹 良洋, 石田 咲子, 井上 佳祐, 齊藤 美咲, 岡橋 啓太郎, 包 昊南, 西田 浩之, 山口 勝司, 重信 秀治, 石崎 公庸, 大和 勝幸, 河内 孝之

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Oral presentation


• 根系の光環境が植物のリン酸応答に与える影響

  吉岡 優介, 大西 美輪, 石崎 公庸, 木下 俊則, 深城 英弘, 三村 徹郎

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• ポプラの短期落葉―開芽系を用いた季節的なリン酸転流機構の解明と野外RNA-seqとの比較

  栗田 悠子, 菅野 里美, 杉田 亮平, 廣瀬 農, 大西 美輪, 手塚 あゆみ, 出口 亜由美, 小菅 桂子, 石崎 公庸, 深城 英弘, 田野井 慶太朗, 中西 友子, 馬場 啓一, 三村 徹郎, 永野 惇

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Oral presentation


• ニチニチソウ乳管細胞・異形細胞の発生・分化・機能の解析

  鵜崎 真妃, 山本 浩太郎, 高橋 勝利, 棟方 涼介, 大西 美輪, 七條 千津子, 石崎 公庸, 深城 英弘, 三村 徹郎

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Oral presentation


• セントポーリア温度降下傷害における機械受容Ca2+チャネルの関与と液胞動態の解析

  本岡 香奈, 大西 美輪, 飯田 和子, 角浜 憲明, 鈴木 祥弘, 石崎 公庸, 深城 英弘, 飯田 秀利, 三村 徹郎

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• ゼニゴケ葉状体の分枝におけるMpCLE2シグナルの役割

  平川 有宇樹, 藤本 童子, 打田 直行, 澤 進一郎, 石崎 公庸, 西浜 竜一, 河内 孝之, 清末 知宏, John Bowman

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Oral presentation


• ゼニゴケの無性芽形成におけるKAR/RopGEFの機能ドメイン解析

  樋渡 琢真, 安居 佑季子, 上野 亜紀, Li Quan Koh, 深城 英弘, 三村 徹郎, 河内 孝之, 浦野 大輔, 石崎 公庸

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• RopGEFの機能ドメイン解析

  樋渡 琢真, 安居 佑季子, 上野 亜紀, Li Quan Koh, 深城 英弘, 三村 徹郎, 河内 孝之, 浦野 大輔, 石崎 公庸

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• ゼニゴケのMYB型転写因子GCAM1による幹細胞制御機構の解析

  安居 佑季子, 塚本 成幸, 西浜 竜一, 深城 英弘, 三村 徹郎, 河内 孝之, 石崎 公庸

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• シロイヌナズナ側根形成能が顕著に低下するfewer roots変異体のサプレッサー変異体の解析

  田井中 芳樹, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• シロイヌナズナ側根形成を抑制するTOLS2ペプチドに対する応答異常変異体の解析

  西丸 陸, 青木 優佳, 雨川 智美, 豊倉 浩一, 篠田 明徳, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• シャジクモ細胞膜リン酸輸送体の分子機能解析

  三村 徹郎, 大西 美輪, 村西 直樹, 藤原 ひとみ, 石崎 公庸, 深城 英弘, 西山 智明, 坂山 英俊, Rob J. Reid, 且原 真木

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Oral presentation


• KAI2シグナル伝達経路はゼニゴケの形態形成を制御する

  水野 陽平, 楢本 悟史, 小松 愛乃, 島崎 翔太, 児玉 恭一, 石崎 公庸, 経塚 淳子

  日本植物学会第82回大会, Sep. 2018, Japanese, 広島国際会議場, Domestic conference

  Poster presentation


• Coordination of lateral organ development and meristem activity mediated by ALOG protein in Marchantia polymorpha

  Satoshi Naramoto, Trozzi Nicola, Jones Victor, Masaki Shimamura, Kanane Sato, Sakiko Ishida, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Junko Kyozuka

  日本植物学会第82回大会, Sep. 2018, English, 広島国際会議場, Domestic conference

  Oral presentation


• 現生の基部植物モデルから陸上植物の共通祖先の表現型をいかに推測するか？

  ISHIZAKI KIMITSUNE

  The 20th Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2018, Japanese, 東京大学駒場Iキャンパス, 陸上植物は約4億7千万年前までに淡水棲緑藻の１グループから派生した単系統のグループである。初期の陸上植物は、現生のコケ植物に近い体制をもっており、次に維管束をもち陸上生活により適したヒカゲノカズラ類やシダ植物の段階、そして種子をもつ種子植物が出現したと考えられている。近年、コケ植物や小葉類、緑藻類のゲノムが相次いで解読され、陸上植物共通祖先がもっていたツールキット遺伝子が推定されつつある。さらに先行のモデルコケ植物である蘚類ヒメツリガネゴケに加え、苔類ゼニゴケについても分子遺伝学の研究基盤が整い、“祖先的な”植物遺伝子の機能とその進化に関する研究が盛んになってきた。一方で、ヒメツリガネゴケとゼニゴケは双方とも“基部植物”ではあるが、分岐して数億年以上も経過しており、コケ植物として共通の形質以外には表現型に大きな違いがある。初期陸上植物はどんな表現型か, Domestic conference

  [Invited]

  Invited oral presentation


• Analysis of lipids accumulated in laticifer and idioblast cells in Catharanthus roseus.

  Mai Uzaki, Kotaro Yamamoto, Katsutoshi Takahashi, Miwa Ohnishi, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

  International Symposium on Plant Lipids, Jul. 2018, English, Yokohama, International conference

  Poster presentation


• The critical role of MpbHLH40 as a positive regulator of dormancy in theliverwort Marchantia polymorpha

  Kimitsune Ishizaki, Mikako Yoshikawa, Shigeyuki Tsukamoto, Hidehiro Fukaki, TetsuroMimura, Daisuke Takezawa, Yoichi Sakata, Takayuki Kohchi

  EMBO Workshop, New shores in land plant evolution, Jun. 2018, English, Lisbon, Portugal, International conference

  Poster presentation


• Germ cell differentiation requires the bHLH transcription factors BONOBOsevolutionarily conserved in land plants

  Shohei Yamaoka, Ryuichi Nishihama, Yoshihiro Yoshitake, Sakiko Ishida, KeisukeInoue, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi

  EMBO Workshop, New shores in land plant evolution, Jun. 2018, English, Lisbon, Portugal, International conference

  Oral presentation


• DICER-LIKE1 Controls Cell Fate Specification In Marchantia polymorpha.

  Adolfo Aguilar-Cruz, Ana Dorantes-Acosta, Bernardo Pollak, Takayuki Kohchi, KimitsuneIshizaki, John Bowman, Daniel Grimanelli, Jim Haseloff, Mario Arteaga-Vázaquez

  EMBO Workshop, New shores in land plant evolution, Jun. 2018, English, Lisbon, Portugal, International conference

  Poster presentation


• Cryopreservation of Marchantia polymorpha spermatozoa

  Taisuke Togawa, Tohru Adachi, Daijiro Harada, Tasuku Mitani, Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato

  EMBO Workshop, New shores in land plant evolution, Jun. 2018, English, Lisbon, Portugal, International conference

  Poster presentation


• Coordination of lateral organ development and stem cell activity inMarchantia polymorpha is mediated by an ALOG family protein

  Satoshi Naramoto, Nicola Trozzi, Victor Jones, Masaki Shimamura, Kanane Sato, Sakiko Ishida, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Liam Dolan, Junko Kyozuka

  EMBO Workshop, New shores in land plant evolution, Jun. 2018, English, Lisbon, Portugal, International conference

  Poster presentation


• Control of meristem organization by local peptide signaling in Marchantiapolymorpha

  Yuki Hirakawa, Naoyuki Uchida, Kimitsune Ishizaki, Ryuichi Nishihama, TakayukiKohchi, Shinichiro Sawa, John Bowman

  EMBO Workshop, New shores in land plant evolution, Jun. 2018, English, Lisbon, Portugal, International conference

  Oral presentation


• ゼニゴケ・ゲノムにおける植物ホルモン生合成・シグナル伝達系遺伝子

  山岡尚平, 西浜竜一, 石崎公庸, 大和勝幸, 河内孝之

  日本農芸化学会大会講演要旨集(Web), Mar. 2018, Japanese


• 陸上植物に共通する水環境依存的な間隙形成の理解

  水谷 未耶, 石崎 公庸, 西浜 竜一, 河内 孝之, 東山 哲也, 金岡 雅浩

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• 転写因子BONOBO は陸上植物の生殖系列細胞の分化に必要である

  山岡 尚平, 西浜 竜一, 吉竹 良洋, 石田 咲子, 井上 佳祐, 齊藤 美咲, 岡橋 啓太郎, 包 昊南, 西田 浩之, 山口 勝司, 重信 秀治, 石崎 公庸, 大和 勝幸, 河内 孝之

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• 根系の光環境が植物のリン酸応答に与える影響

  吉岡 優介, 大西 美輪, 石崎 公庸, 木下 俊則, 深城 英弘, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケの仮根細胞における微小管依存的な先端成長機構の解析

  本瀬 宏康, 大谷 健人, 石崎 公庸, 高谷 彰吾, 西浜 竜一, 河内 孝之, 高橋 卓

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• ニチニチソウ乳管細胞および異形細胞の分化機構の解明

  鵜崎 真妃, 山本 浩太郎, 高橋 勝利, 大西 美輪, 石崎 公庸, 深城 英弘, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• セントポーリアの温度降下感受性に関わる分子機構の解析

  本岡 香奈, 大西 美輪, 飯田 和子, 角浜 憲明, 鈴木 祥弘, 石崎 公庸, 深城 英弘, 飯田 秀利, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケ無性芽形成初期を制御するRop シグナル伝達経路の機能解析

  樋渡 琢真, Li Quan Koh, 深城 英弘, 三村 徹郎, 河内 孝之, 浦野 大輔, 石崎 公庸

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケ無性芽の休眠を正に制御するMpbHLH40の機能解析

  吉川 実樺子, 塚本 成幸, 深城 英弘, 三村 徹郎, 竹澤 大輔, 坂田 洋一, 河内 孝之, 石崎 公庸

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケを用いたD14/KAI2 シグナル伝達経路の根源的機能の解析

  水野 陽平, 楢本 悟史, 石崎 公庸, 経塚 淳子

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• ゼニゴケMarchantia polymorphaにおけるリン欠乏時の膜脂質転換機構の解析

  中村 将, 北浦 銀河, 佐々木(関本, 結子, 堀 孝一, 岩井 雅子, 太田 啓之, 石崎 公庸, 下嶋 美恵

  第58回日本植物生理学会年会, Mar. 2018, Japanese, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• ゼニゴケのサーモスペルミン合成酵素遺伝子MpACL5機能欠損変異株の解析

  古本拓也, 大谷健人, 石崎公庸, 山岡尚平, 河内孝之, 本瀬宏康

  日本ポリアミン学会年会プログラム及び抄録集, Jan. 2018, Japanese


• 転写因子BONOBOは陸上植物の生殖系列細胞の分化に必要である

  山岡尚平, 西浜竜一, 吉竹良洋, 石田咲子, 井上佳祐, 齊藤美咲, 岡橋啓太郎, BAO Haonan, 西田浩之, 山口勝司, 重信秀治, 石崎公庸, 大和勝幸, 河内孝之

  日本植物生理学会年会(Web), 2018, Japanese


• ゼニゴケ〜温故知新〜その２

  石崎 公庸

  しだとこけ談話会 第１８９回会合, Dec. 2017, Japanese, 大阪市立自然史博物館, Domestic conference

  [Invited]

  Invited oral presentation


• Regulation and Biosynthesis of Phenolics in M. polymorpha

  Hiroyoshi Kubo, Shunsuke Nozawa, Takuma Hiwatashi, Youichi Kondou, Ryo Nakabayashi, Tetsuya Mori, Kazuki Saito, Kojiro Takanashi, Takayuki Kohchi, Kimitsune Ishizaki

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• Molecular characterization of plasma membrane H+-ATPase in Marchantia polymorpha

  Kotaro Nakane, Masaki Okumura, Tameo You, Kimitsune Ishizaki, Takayuki Kohchi, Toshinori Kinoshita

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• Microtubule-dependent directional growth of rhizoids in Marchantia polymorpha

  Hiroyasu Motose, Kento Otani, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Taku Takahashi

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• Local CLE peptide signaling in the Marchantia polymorpha meristem

  Yuki Hirakawa, Naoyuki Uchida, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Shinichiro Sawa, John L. Bowman

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• KARAPPO encoding RopGEF is critical for the gemma initial development in the liverwort Marchantia polymorpha

  Takuma Hiwatashi, Koh Li Quan, Katsushi Yamaguchi, Shuji Shigenobu, Shinichiro Sawa, Hiroyuki Kirita, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, Daisuke Urano, Kimitsune Ishizaki

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• Gemma and Gemma Cup Development in Marchantia polymorpha

  Kimitsune Ishizaki

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  [Invited]

  Invited oral presentation


• Evolution of Pi-sensing along the plant kingdom

  Félix Rico-Resendiz, Alan Cervantes-Perez, Aracely Oropeza-Aburto, Kimitsune Ishizaki, Mario Arteaga-Vazquez, Alfredo Cruz-Ramirez, Luis Herrera-Estrella

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• DICER-LIKE1 Controls Cell Fate Specification In Marchantia polymorpha

  Adolfo Aguilar-Cruz, Ana Dorantes-Acosta, Bernardo Pollak, Takayuki Kohchi, Kimitsune Ishizaki, John Bowman, Daniel Grimanelli, Jim Haseloff, Mario Arteaga-Vázquez

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Oral presentation


• BONOBOs are evolutionarily conserved transcription factors required for germ cell fate determination in land plants

  Shohei Yamaoka, Ryuichi Nishihama, Yoshihiro Yoshitake, Sakiko Ishida, Keisuke Inoue, Misaki Saito, Keitaro Okahashi, Haonan Bao, Hiroyuki Nishida, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• Analysis of Air Chamber Development in Marchantia polymorpha

  Miya Mizutani, Kimitsune Ishizaki, Yoichiro Fukao, Masayuki Fujiwara, Ryuichi Nishihama, Takayuki Kohchi, Tetsuya Higashiyama, Masahiro Kanaoka

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• A gene encoding LRR-RLK is involved in OPDA signaling of Marchantia polymorpha

  Yuka Konishi, Jun Oshika, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi, Hideyuki Matsuura, Kosaku Takahashi

  The 65th NIBB conference ーMarchantia Workshop 2017−, Dec. 2017, English, 基礎生物学研究所（National Institute for Basic Biology), International conference

  Poster presentation


• Molecular mechanism of vegetative reproduction in the liverwort Marchantia polymorpha

  Kimitsune Ishizaki

  Taiwan-Japan Plant Biology 2017, Nov. 2017, English, Academia Sinica, International conference

  [Invited]

  Invited oral presentation


• Mechanism of cell injury induced by a rapid temperature decrease in Saintpaulia sp. leaves.

  Miwa Ohnishi, Kana Motooka, Noriaki Kadohama, Kazuko Iida, Kimitsune Ishizaki, Hidehiro Fukaki, Yoshihiro Suzuki, Hidetoshi Iida, Tetsuro Mimura

  Taiwan-Japan Plant Biology 2017, Nov. 2017, English, Academia Sinica, International conference

  Poster presentation


• KARAPPO encoding RopGEF is critical for the gemma development in the liverwort Marchantia polymorpha

  Takuma Hiwatashi, Koh Li Quan, Katsushi Yamaguchi, Shuji Shigenobu, Shinichiro Sawa, Hiroyuki Kirita, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, Daisuke Urano, Kimitsune Ishizaki

  Taiwan-Japan Plant Biology 2017, Nov. 2017, English, Academia Sinica, International conference

  Poster presentation


• Idioblast and laticifer cells play important roles in alkaloid biosynthesis in Catharanthus plants

  Kotaro Yamamoto, Katsutoshi Takahashi, Lorenzo Caputi, Hajime Mizuno, Miwa Ohnishi, Kimitsune Ishizaki, Mami Yamazaki, Hidehiro Fukaki, Sarah O’Connor, Tetsuro Mimura

  Taiwan-Japan Plant Biology 2017, Nov. 2017, English, Academia Sinica, International conference

  Poster presentation


• Functional characterization of MpbHLH40 in gemma dormancy of a liverwort Marchantia polymorpha

  Mikako Yoshikawa, Shigeyuki Tsukamoto, Hidehiro Fukaki, Tetsuro Mimura, Daisuke Takezawa, Yohichi Sakata, Kohchi Takayuki, Kimitsune Ishizaki

  Taiwan-Japan Plant Biology 2017, Nov. 2017, English, Academia Sinica, International conference

  Oral presentation


• A shortened annual cycle system; a tool for laboratory studies of seasonal phenomena in trees

  Yuko Kurita, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Ayumi Tezuka, Ayumi Deguchi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Keitaro Tanoi, Tomoko M. Nakanishi, Kei’ichi Baba, Tetsuro Mimura, Atsushi J. Nagano

  Taiwan-Japan Plant Biology 2017, Nov. 2017, English, Academia Sinica, International conference

  Poster presentation


• Seasonal Pre-translocation in the deciduous poplar tree.

  ○Yuko Kurita, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Ayumi Tezuka, Ayumi Deguchi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Keitaro Tanoi, Tomoko M. Nakanishi, Kei’ichi Baba, Tetsuro Mimura, Atsushi J. Nagano

  THE DEVELOPING PLANT IN ITS ENVIRONMENT, Oct. 2017, English, Lyon, France, International conference

  Poster presentation


• Mechanism of cell injury induced by a rapid temperature decrease in Saintpaulia sp. leaves.

  Miwa Ohnishi, Kana Motooka, Noriaki Kadohama, Kazuko Iida, Kimitsune Ishizaki, Hidehiro Fukaki, Yoshihiro Suzuki, Hidetoshi Iida, Tetsuro Mimura

  THE DEVELOPING PLANT IN ITS ENVIRONMENT, Oct. 2017, English, Domaine Lyon Saint-Joseph, International conference

  Poster presentation


• Idioblast and laticifer cells play important roles in alkaloid biosynthesis in Catharanthus roseus.

  ○Kotaro Yamamoto, Katsutoshi Takahashi, Lorenzo Caputi, Hajime Mizuno, Miwa Ohnishi, Kimitsune Ishizaki, Mami Yamazaki, Hidehiro Fukaki, Sarah E O’Connor, Tetsuro Mimura

  THE DEVELOPING PLANT IN ITS ENVIRONMENT, Oct. 2017, English, Lyon, France, International conference

  Poster presentation


• Critical role of KARAPPO/RopGEF in the initial stage of gemma formation in the liverwort Marchantia polymorpha

  Kimitsune Ishizaki

  GDRI-Integrative Plant Biology `The Developing Plant in Its Environment-, Oct. 2017, English, Domaine Lyon Saint-Joseph, Lyon, France, International conference

  Poster presentation


• ゼニゴケの活性酸素生成酵素Rbohのストレス応答における生理機能の探索

  進藤大輝, 橋本研志, 北畑信隆, 北畑信隆, 西浜竜一, 石崎公庸, 河内孝之, 朽津和幸, 朽津和幸

  日本植物学会大会研究発表記録, Sep. 2017, Japanese


• 落葉木本植物ポプラの季節的なリン転流機構の解析

  栗田 悠子, 菅野 里美, 杉田 亮平, 廣瀬 農, 大西 美輪, 手塚 あゆみ, 出口 亜由美, 小菅 桂子, 石崎 公庸, 深城 英弘, 田野井 慶太朗, 中西 友子, 馬場 啓一, 永野 惇, 三村 徹郎

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• 植物界の両生類？−コケ植物ゼニゴケの生き方

  石崎 公庸

  神戸大学重点チームシンポジウム「水環境における生物の適応戦略」, Sep. 2017, Japanese, 神戸大学-淡路島内海域環境教育研究センターマリンサイト, Domestic conference

  [Invited]

  Invited oral presentation


• 根系の光環境が植物のリン応答に及ぼす影響

  吉岡 優介, 大西 美輪, 石崎 公庸, 深城 英弘, 三村 徹郎

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• 汽水産緑藻 Ulva compressa のNa+に依存した成長とリン酸の取り込みについて

  井阪 若菜, 寺内 真, 大西 美輪, 羽生田 岳明, 市原 健介, 山崎 誠和, 石崎 公庸, 深城 英弘, 河野 重行, 川井 浩史, 新免 輝男, 三村 徹郎

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケにおけるリン酸応答機構

  石崎 公庸

  第３回植物の栄養研究会, Sep. 2017, Japanese, 東京工業大学, Domestic conference

  Oral presentation


• 基部陸上植物ゼニゴケにおけるリン酸に応答した遺伝子転写制御

  北浦 銀河, 三村 徹郎, 深城 英弘, 石崎 公庸

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• 活性酸素生成酵素Rbohはゼニゴケの発生・形態形成において細胞表層構造の調節に関わる

  橋本 研志, 高川 智弘, 木村 貴史, 春日谷 海, 賀屋 秀隆, 北畑 信隆, 石崎 公庸, 西浜 竜一, 河内 孝之, 朽津 和幸

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• セントポーリアの温度感受性に関わる分子機構の解析

  本岡 香奈, 大西 美輪, 飯田 和子, 角浜 憲明, 鈴木 祥弘, 石崎 公庸, 深城 英弘, 飯田 秀利, 三村 徹郎

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• ゼニゴケ無性芽発生を制御するRopGEF/KARの生化学的解析

  樋渡 琢真, Li Quan Koh, 深城 英弘, 三村 徹郎, 河内 孝之, 浦野 大輔, 石崎 公庸

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• ゼニゴケ気室が水環境の変動に応答して密度変化する分子機構の解析

  水谷 未耶, 石崎 公庸, 深尾 陽一朗, 藤原 正幸, 西浜 竜一, 河内 孝之, 東山 哲也, 金岡 雅浩

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケの生殖における活性酸素生成酵素Rbohの役割

  高川 智弘, 木村 貴史, 橋本 研志, 北畑 信隆, 石崎 公庸, 西浜 竜一, 河内 孝之, 朽津 和幸

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケの活性酸素生成酵素Rbohのストレス応答における生理機能の探求

  進藤 大輝, 橋本 研志, 北畑 信隆, 西浜 竜一, 石崎 公庸, 河内 孝之, 朽津 和幸

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケから見た微小管関連遺伝子の機能と進化

  本瀬 宏康, 大谷 健人, 倉田 元気, 高谷 彰吾, 石崎 公庸, 西浜 竜一, 河内 孝之, 高橋 卓

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケALOGドメイン遺伝子MpTAW1の進化発生学的解析

  楢本 悟史, 石崎 公庸, 嶋村 正樹, 徳永 浩樹, 吉田 明希子, 西浜 竜一, 河内 孝之, 経塚 淳子

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Oral presentation


• シロイヌナズナ側根形成を抑制するTOLS2類似ペプチドの解析

  久内 彩洋子, 豊倉 浩一, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• シロイヌナズナ側根形成を抑制するTOLS2ペプチドに対する高感受性変異体の解明

  青木 優佳, 豊倉 浩一, 篠田 明徳, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• さまざまな植物における紅葉・黄葉現象の解析

  岩村 青子, 栗田 悠子, 大西 美輪, 藤枝 聡志, 鈴木 留依, 出口 亜由美, 手塚 あゆみ, 小菅 桂子, 石崎 公庸, 深城 英弘, 永野 惇, 馬場 啓一, 飯野 盛利, 三村 徹郎

  日本植物学会第81回大会, Sep. 2017, Japanese, 東京理科大学 野田キャンパス, Domestic conference

  Poster presentation


• Molecular genetics of the gametophytic body plan in a basal land plant Marchantia polymorpha

  Kimitsune Ishizaki

  editBio 2017, 1st International Symposium and Training Course on Genome Engineering & Developmental Biology, Aug. 2017, English, Irapuato, Guanajuato. México, International conference

  [Invited]

  Invited oral presentation


• ゼニゴケ〜温故知新〜その１

  石崎 公庸

  しだとこけ談話会 第１８７回会合, Jun. 2017, Japanese, 大阪市立自然史博物館, Domestic conference

  [Invited]

  Invited oral presentation


• The transcription factor BONOBO controls sexual organ development in the basal land plant Marchantia polymorpha

  Shohei Yamaoka, Keisuke Inoue, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi

  50th Annual Meeting of the Japanese Society of Developmental Biologists, May 2017, English


• 根の障害物認識機構の解明

  吉田 峻一, 大西 美輪, 石崎 公庸, 深城 英弘, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• 汽水産緑藻 Ulva proliferaの Na+ に依存した成長とリン酸の取り込みについて

  井阪 若菜, 大西 美輪, HANYUDA TAKEAKI, 市原 健介, 山崎誠和, 石崎 公庸, 深城 英弘, 河野 重行, KAWAI HIROSHI, 新免 輝男, 三村 徹郎

  日本藻類学会第41回大会, Mar. 2017, Japanese, 高知, Domestic conference

  Oral presentation


• 基部陸上植物におけるUV-B 耐性に関するシグナル伝達系の解析

  森山 亜沙美, 藤平 健太, 森戸 健, 李 天虹, 西浜 竜一, 山岡 尚平, 石崎 公庸, 久保 浩義, 河内 孝之, 近藤 陽一

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• 基部陸上植物におけるPSI 防御戦略: ゼニゴケにおいてFlavodiironタンパク質はP700 酸化に働く

  嶋川 銀河, 石崎 公庸, 田中 萌子, 高木 大輔, 塙 仁美, 三宅 親弘

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• 基部陸上植物ゼニゴケのUV-B 受容体MpUVR8 の機能解析

  宮内 渉, 武井 翔太, 森戸 健, 宮城 祐太, 岩渕 功誠, 西浜 竜一, 石崎 公庸, 西村 いくこ, 河内 孝之, 近藤 陽一

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• ポプラの短期落葉- 開芽系を用いた季節的なリン転流機構の解析

  栗田 悠子, 菅野 里美, 杉田 亮平, 廣瀬 農, 大西 美輪, 手塚 あゆみ, 永野 惇, 小菅 桂子, 石崎 公庸, 深城 英弘, 田野井 慶太朗, 中西 友子, 馬場 啓一, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ニチニチソウ異形細胞・乳管細胞におけるTIA 代謝機構の解析

  山本 浩太郎, 大西 美輪, 高橋 勝利, 水野 初, 石崎 公庸, 山崎 真巳, 深城 英弘, 升島 努, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• なぜセントポーリアは急激な温度降下に敏感なのか？

  大西 美輪, 角浜 憲明, 石崎 公庸, 深城 英弘, 鈴木 祥弘, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケ無性芽の休眠に関連するbHLH 遺伝子の機能解析

  吉川 実樺子, 塚本 成幸, 深城 英弘, 三村 徹郎, 竹澤 大輔, 坂田 洋一, 石崎 公庸

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケ微小管関連遺伝子の機能解析

  本瀬 宏康, 大谷 健人, 石崎 公庸, 西浜 竜一, 河内 孝之, 高橋 卓

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• ゼニゴケ配偶体の成長における活性酸素生成酵素MpRbohAとMpRbohB の異なる役割

  橋本 研志, 木村 貴史, 春日谷 海, 賀屋 秀隆, 北畑 信隆, 石崎 公庸, 西浜 竜一, 河内 孝之, 朽津 和幸

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケ杯状体形成に重要なGEMMA CUP-ASSOCIATED MYB2の機能

  高見 英幸, 塚本 成幸, 増田 晃秀, 深城 英弘, 三村 徹郎, 河内 孝之, 石崎 公庸

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• ゼニゴケ精子形成過程を通じた中心体タンンパクの細胞内局在変化について

  野村 佳織, 嶋村 正樹, ISHIZAKI KIMITSUNE

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケの精子形成・機能に関わる膜交通システムの解析

  南野 尚紀, 金澤 建彦, 法月 拓也, 西浜 竜一, 大和 勝幸, 石崎 公庸, 河内 孝之, 中野 明彦, 上田 貴志

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケの光屈性には成長段階に応じて異なる光シグナル伝達因子が関与する

  小松 愛乃, 末次 憲之, 西浜 竜一, 石崎 公庸, 河内 孝之

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケにおける細胞膜H+-ATPase の生理的機能の解析

  中根 功多朗, 奥村 将樹, 楊 為雄, 井上 晋一郎, 石崎 公庸, 河内 孝之, 木下 俊則

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• ゼニゴケにおけるサーモスペルミン合成酵素遺伝子MpACL5の機能解析

  古本 拓也, 大谷 健人, 石崎 公庸, 山岡 尚平, 河内 孝之, 本瀬 宏康, 高橋 卓

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• ゼニゴケにおけるカリキンシグナル伝達機構

  水野 陽平, 楢本 悟史, 石崎 公庸, 経塚 淳子

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• ゼニゴケALOGドメイン遺伝子MpTAW1の機能解析

  楢本 悟史, 石崎 公庸, 嶋村 正樹, 石田 咲子, 徳永 浩樹, 吉田 明希子, 西浜 竜一, 河内 孝之, 経塚 淳子

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• シロイヌナズナ側根形成に関わるTOLS2 ペプチドによる遺伝子の発現制御に異常を示す変異体の解析

  青木 優佳, 豊倉 浩一, 篠田 明徳, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• シロイヌナズナのリン酸濃度応答遺伝子の探索／第58回日本植物生理学会年会

  鈴木 太郎, 大西 美輪, 菅野 里美, 手塚 あゆみ, 出口 亜由美, 永野 惇, 石川 亮, 石崎 公庸, 深城 英弘, 三村 徹郎

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Oral presentation


• Marchantia polymorphaでの無性芽形成初期におけるRopGEF 遺伝子、KARAPPOの重要な役割

  樋渡 琢真, 山口 勝司, 重信 秀治, 澤 進一郎, 桐田 啓如, 深城 英弘, 三村 徹郎, 河内 孝之, 石崎 公庸

  第58回日本植物生理学会年会, Mar. 2017, Japanese, 鹿児島, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケのUV‐B耐性に関するシグナル伝達系の解析

  森山亜沙美, 森戸健, 李天虹, 石崎公庸, 近藤陽一, 藤平健太, 宮城祐太, 西浜竜一, 河内孝之

  関東学院大学理工/建築・環境学会研究発表講演論文集, 2017, Japanese


• 基部陸上植物ゼニゴケのUV‐B受容体MpUVR8の機能解析

  宮内渉, 森戸健, 岩渕功誠, 石崎公庸, 河内孝之, 武井翔太, 宮城祐太, 西浜竜一, 西村いくこ, 近藤陽一

  関東学院大学理工/建築・環境学会研究発表講演論文集, 2017, Japanese


• 基部陸上植物及び高等植物を用いた高温・塩耐性を付与する有用遺伝子の探索

  飯沼弘太朗, 小林美由希, 宮城祐太, 石崎公庸, 二瓶遼太, 寺沢拓弥, 西浜竜一, 河内孝之, 近藤陽一

  関東学院大学理工/建築・環境学会研究発表講演論文集, 2017, Japanese


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  OHNISHI MIWA, 本岡 香奈, 角浜 憲明, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 鈴木 祥弘, MIMURA TETSURO

  近畿植物学会講演会, Nov. 2016, Japanese, 兵庫県民会館, Domestic conference

  Oral presentation


• シロイヌナズナのリン酸濃度応答遺伝子の探索

  鈴木 太郎, OHNISHI MIWA, 菅野 里美, 豊倉 浩一, 郷 達明, 手塚 あゆみ, 永野 惇, 石川 亮, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  近畿植物学会講演会, Nov. 2016, Japanese, 兵庫県民会館, Domestic conference

  Oral presentation


• ゼニゴケ無性芽形成異常変異体karappoの解析

  樋渡 琢真, ISHIZAKI KIMITSUNE

  新学術領域研究「植物発⽣ロジック」若⼿ワークショップ2016, Oct. 2016, Japanese, ラフォーレ修善寺, Domestic conference

  Oral presentation


• ゼニゴケ杯状体形成に関与するGCAM2遺伝⼦の同定と解析

  高見 英幸, ISHIZAKI KIMITSUNE

  新学術領域研究「植物発⽣ロジック」若⼿ワークショップ2016, Oct. 2016, Japanese, ラフォーレ修善寺, Domestic conference

  Oral presentation


• ゼニゴケにおけるリン酸応答機構の研究

  北浦 銀河, ISHIZAKI KIMITSUNE

  新学術領域研究「植物発⽣ロジック」若⼿ワークショップ2016, Oct. 2016, Japanese, ラフォーレ修善寺, Domestic conference

  Oral presentation


• シロイヌナズナのリン酸濃度応答遺伝子の探索

  鈴木太郎, 大西美輪, 菅野里美, 郷達明, 豊倉浩一, 永野惇, 手塚あゆみ, 石川亮, 石崎公庸, 深城英弘, 三村徹郎

  日本植物学会大会研究発表記録, Sep. 2016, Japanese


• ポプラにおける葉組織リン代謝の季節変動―野外と実験室培養系の比較―

  岩村青子, 栗田悠子, 馬場啓一, 大西美輪, 手塚あゆみ, 永野惇, 小菅桂子, 石崎公庸, 深城英弘, 三村徹郎

  日本植物学会大会研究発表記録, Sep. 2016, Japanese


• ポプラの短期落葉‐開芽系を用いた季節的なリン転流機構の解析

  栗田悠子, 菅野里美, 杉田亮平, 廣瀬農, 大西美輪, 手塚あゆみ, 永野惇, 小菅桂子, 石崎公庸, 深城英弘, 田野井慶太朗, 田野井慶太朗, 中西友子, 馬場啓一, 三村徹郎

  日本植物学会大会研究発表記録, Sep. 2016, Japanese


• ゼニゴケのストレス応答におけるROS生成酵素Rbohの機能解析

  進藤大輝, 橋本研志, 北畑信隆, 西浜竜一, 石崎公庸, 河内孝之, 朽津和幸

  日本植物学会大会研究発表記録, Sep. 2016, Japanese


• ゼニゴケX染色体に存在するREPRESSOR OF SILENCING 1ホモログMpROS1Xの機能解析

  十川太輔, 原田大士朗, 池田陽子, 塚本成幸, 石崎公庸, 丹羽優喜, 荒木崇, 山口勝司, 重信秀治, 河内孝之, 大和勝幸

  日本植物学会大会研究発表記録, Sep. 2016, Japanese


• ゼニゴケの発生・形態形成におけるROS生成酵素Rbohの機能解析

  橋本研志, 木村貴史, 籔田渉二, 山田融, 賀屋秀隆, 北畑信隆, 西浜竜一, 石崎公庸, 河内孝之, 朽津和幸

  日本植物学会大会研究発表記録, Sep. 2016, Japanese


• 植物の雄性配偶子形成に関する転写因子の進化：苔類ゼニゴケを用いた研究によりわかってきたこと

  肥後 あすか, 河島 友和, 石崎 公庸, 大和 勝幸, 河内 孝之, Frederic Berger, 荒木 崇

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  [Invited]

  Invited oral presentation


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  大西 美輪, 角浜 憲明, 石崎 公庸, 深城 英弘, 鈴木 祥弘, 三村 徹郎

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ポプラの短期落葉- 開芽系を用いた季節的なリン転流機構の解析

  栗田 悠子, 菅野 里美, 杉田 亮平, 廣瀬 農, 大西 美輪, 手塚 あゆみ, 永野 惇, 小菅 桂子, 石崎 公庸, 深城 英弘, 田野井 慶太朗, 中西 友子, 馬場 啓一, 三村 徹郎

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ポプラにおける葉組織リン代謝の季節変動 - 野外と実験室培養系の比較-

  岩村 青子, 栗田 悠子, 馬場 啓一, 大西 美輪, 手塚 あゆみ, 永野 惇, 小菅 桂子, 石崎 公庸, 深城 英弘, 三村 徹郎

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ニチニチソウ異形細胞におけるTIA 代謝分化機構の解析

  山本 浩太郎, 高橋 勝利, 水野 初, 大西 美輪, 江崎 剛史, 石崎 公庸, 深城 英弘, 山崎 真巳, 升島 努, 三村 徹郎

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケ無性芽形成におけるLATERAL SUPPRESSOR 相同遺伝子の機能

  宇野 真里奈, 塚本 成幸, 深城 英弘, 三村 徹郎, 石崎 公庸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケ無性芽の休眠に関連するbHLH遺伝子の機能解析

  吉川 実樺子, 塚本 成幸, 深城 英弘, 三村 徹郎, 石崎 公庸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケ配偶体の幹細胞増殖を制御するメカニズム

  石崎 公庸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  [Invited]

  Invited oral presentation


• ゼニゴケの発生・形態形成におけるROS生成酵素Rboh の機能解析

  橋本 研志, 木村 貴史, 籔田 渉二, 山田 融, 賀屋 秀隆, 北畑 信隆, 西浜 竜一, 石崎 公庸, 河内 孝之, 朽津 和幸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケの栄養繁殖器官におけるトランスクリプトーム解析

  塚本 成幸, 大和 勝幸, 山口 勝司, 重信 秀治, 深城 英弘, 三村 徹郎, 河内 孝之, 石崎 公庸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケのフェノール性化合物蓄積に関わるR2R3-MYB 遺伝子の発現解析

  久保 浩義, 野澤 俊介, 近藤 陽一, 石崎 公庸, 高梨 功次郎, 河内 孝之

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケのストレス応答におけるROS 生成酵素Rbohの機能解析

  進藤 大輝, 橋本 研志, 北畑 信隆, 西浜 竜一, 石崎 公庸, 河内 孝之, 朽津 和幸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゼニゴケX 染色体に存在するREPRESSOR OF SILENCING 1 ホモログMpROS1X の機能解析

  十川 太輔, 原田 大士朗, 池田 陽子, 塚本 成幸, 石崎 公庸, 丹羽 優喜, 荒木 崇, 山口 勝司, 重信 秀治, 河内 孝之, 大和 勝幸

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• シロイヌナズナ側根形成に関わるTOLS2ペプチドによるPUCHI 遺伝子の発現制御機構の解析

  青木 優佳, 豊倉 浩一, 篠田 明徳, 郷 達明, 石崎 公庸, 三村 徹郎, 深城 英弘

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• シロイヌナズナのリン酸濃度応答遺伝子の探索／日本植物学会 第80回大会

  鈴木 太郎, 大西 美輪, 菅野 里美, 郷 達明, 豊倉 浩一, 永野 惇, 手塚 あゆみ, 石川 亮, 石崎 公庸, 深城 英弘, 三村 徹郎

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• ゲノム編集を用いたゼニゴケ微小管関連遺伝子の機能解析

  本瀬 宏康, 大谷 健人, 石崎 公庸, 西浜 竜一, 河内 孝之, 高橋 卓

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• Ulva prolifera のナトリウム依存性の成長量変化について

  井阪 若菜, 大西 美輪, HANYUDA TAKEAKI, 市原 健介, 石崎 公庸, 深城 英弘, 河野 重行, KAWAI HIROSHI, 新免 輝男, 三村 徹郎

  日本植物学会 第80回大会, Sep. 2016, Japanese, 沖縄, Domestic conference

  Poster presentation


• The transcription factor BONOBO plays a central role in transition from vegetative to reproductive growth in the liverwort Marchantia polymorpha

  Shohei Yamaoka, Keisuke Inoue, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi

  EMBO Workshop New model systems for early land plant evolution, Jun. 2016, English


• Molecular genetics of gemma and gemma-cup development in the liverwort Marchantia polymorpha

  Kimitsune Ishizaki

  EMBO workshop "New model systems for early land plant evolution", Jun. 2016, English, Vienna, International conference

  [Invited]

  Invited oral presentation


• Critical role of the R2R3-MYB gene GEMMA CUP-ASSOCIATED MYB1 for vegetative propagation in the liverwort Marchantia polymorpha L.

  Shigeyuki Tsukamoto, Tomomi Sugaya, Katsuyuki T. Yamato, Ryuichi Nishihama, Hiroyoshi Kubo, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, Kimitsune Ishizaki

  EMBO workshop "New model systems for early land plant evolution", Jun. 2016, English, Vienna, Many plants have an ability to generate clonal progenies directly from somatic cells of vegetative organs, however, little is known about the molecular mechanism. The liverwort Marchantia polymorpha performs vegetative propagation via gemmae generated in the gemma cups formed on the dorsal side of thallus. To investigate regulatory genes involved in the process of gemma and gem, International conference

  Poster presentation


• 古い酒を新しい革袋に～preexisting gene regulatory network の転用による 陸上植物のボディプラン革新

  ISHIZAKI KIMITSUNE, SAKAKIBARA KEIKO

  BSJ-Review, Apr. 2016, Japanese, 日本植物学会

  [Invited]


• 植物組織における低分子量物質分布の質量顕微鏡による可視化

  高橋勝利, 姉川彩, OHNISHI MIWA, 山本浩太郎, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  Plant Morphology, Apr. 2016, Japanese

  [Invited]


• Evolutional analysis of phosphate transport mechanism in plant cells.

  FUJIWARA Hitomi, SAKAYAMA Hidetoshi, OHNISHI Miwa, ISHIZAKI Kimitsune, TOYOKURA Koichi, GOH Tatsuaki, SEKIMOTO Hiroyuki, NISHIYAMA Tomoaki, IKEGAYA Hisato, KANNO Satamoi, TEZUKA Ayumi, NAGANO Atsushi J, KOSUGE Keiko, FUKAKI Hidehiro, MIMURA Tetsuro

  日本植物生理学会年会要旨集, Mar. 2016, English


• The function of an R2R3-MYB on the development of epidermal tissues of Marchantia polymorpha.

  Ayano Morikoshi, Kimitsune Ishizaki, Hiroyoshi Kubo

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Terpenoid indole alkaloids accumulated in Catharanthus roseus idioblast and laticifer cells

  Kotaro Yamamoto, Miwa Ohnishi, Katsutoshi Takahashi, Hajime Mizuno, Tsuyoshi Esaki, Kimitsune Ishizaki, Mami Yamazaki, Hidehiro Fukaki, Tsutomu Masujima, Tetsuro Mimura

  （一社）日本植物生理学会第57回年会, Mar. 2016, Japanese, 盛岡, Domestic conference

  Poster presentation


• Studies of GUN1 plastid signaling.

  Nobuyoshi Mochizuki, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Akira Nagatani

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Seasonal changes in phosphorus metabolites in leaves of the deciduous woody plant, Populus alba

  Shoko Iwamura, Yuko Kurita, Kei’ichi Baba, Miwa Ohnishi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

  （一社）日本植物生理学会第57回年会, Mar. 2016, Japanese, 盛岡, Domestic conference

  Poster presentation


• Screen for regulatory proteins of schizogenous intercellular space formation in Marchantia polymorpha by using mass spectrometry and the CRISPR/Cas9 genome editing technology.

  Miya Mizutani, Kimitsune Ishizaki, Yoichiro Fukao, Masayuki Fujiwara, Akihide Masuda, Ryuichi Nishihama, Takayuki Kohchi

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Re-translocation of nutrients during heartwood formation of Poplar tree

  Shun Manabe, Yuko Kurita, Kei’ichi Baba, Kotaro Yamamoto, Katsutoshi Takahashi, Miwa Ohnishi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

  （一社）日本植物生理学会第57回年会, Mar. 2016, Japanese, 盛岡, Domestic conference

  Oral presentation


• Profiling and characterization of microRNAs in the liverwort, Marchantia polymorpha

  Masayuki Tsuzuki, Ryuichi Nishihama, Kimitsune Ishizaki, Yukio Kurihara, Minami Matsui, John L. Bowman, Takayuki Kohchi, Takahiro Hamada, Yuichiro Watanabe

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Physiological study of the response to thermospermine in Marchantia polymorpha.

  Takuya Furumoto, Kento Otani, Kimitsune Ishizaki, Takayuki Kohchi, Hiroyasu Motose

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Molecular mechanism of the PIF-mediated red light signaling in the liverwort Marchantia polymorpha

  Keisuke Inoue, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Investigation of molecular mechanisms for UV-B sensing in Marchantia polymorpha.

  Kenta Fujihira, Asami Moriyama, Wataru Miyauchi, Takeshi Morito, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi, Youichi Kondou

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Identification of the gene GEMMA CUP-ASSOCIATED MYB 2 involved in the formation of gemma cup in Marchantia polymorpha

  Hideyuki Takami, Shigeyuki Tsukamoto, Akihide Masuda, FUKAKI Hidehiro, MIMURA TETSURO, Takayuki Kohchi, ISHIZAKI Kimitsune

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Identification and characterization of a bHLH gene involved in gemma germination in the liverwort Marchantia polymorpha.

  Mikako Yoshikawa, Shigeyuki Tsukamoto, FUKAKI Hidehiro, MIMURA TETSURO, ISHIZAKI Kimitsune

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Functional diversification of SYP1 members in Marchantia polymorpha.

  Takehiko Kanazawa, Atsuko Era, Naoki Minamino, Hatsune Morinaka, Takuya Norizuki, Masaru Fujimoto, Kazuhiro Uemura, Ryuichi Nishihama, Katsuyuki T. Yamato, Kimitsune Ishizaki, Tomoaki Nishiyama, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Functional characterization of GCAM1, an R2R3-MYB essential for the development of gemma cup in the liverwort Marchantia polymorpha.

  Shigeyuki Tsukamoto, Tomomi Sugaya, Katsuyuki T. Yamato, Ryuichi Nishihama, Hiroyoshi Kubo, FUKAKI Hidehiro, MIMURA TETSURO, Takayuki Kohchi, ISHIZAKI Kimitsune

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Functional characterization of a LATERAL SUPPRESSOR homolog in Marchantia polymorpha.

  Marina Uno, Shigeyuki Tsukamoto, FUKAKI Hidehiro, MIMURA TETSURO, ISHIZAKI Kimitsune

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Functional analysis of ALOG family protein in Marchantia polymorpha.

  Satochi Naramoto, Kimitsune Ishizaki, Masaki Shimamura, Hiroki Tokunaga, Akiko Yoshida, Shigeyuki Tsukamoto, Hideyuki Takami, Ryuichi Nishihama, Takayuki Kohchi, Junko Kyozuka

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Functional analysis of ABI3 in Marchantia polymorpha using gene targeting

  Masakazu Kanei, Ryousuke Komatsu, Daisuke Onno, Hagiwara Yu, Kenji Komatsu, Daisuke Takezawa, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi, Teruaki Taji, Takahisa Hayashi, Yoichi Sakata

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Evolutional analysis of phosphate transport mechanism in plant cells

  Hitomi Fujiwara, Hidetoshi Sakayama, Miwa Ohnishi, Kimitsune Ishizaki, Koichi Toyokura, Tatsuaki Goh, Hiroyuki Sekimoto, Tomoaki Nishiyama, Hisato Ikegaya, Satamoi Kanno, Ayumi Tezuka, Atsushi J. Nagano, Keiko Kosuge, Hidehiro Fukaki, Tetsuro Mimura

  （一社）日本植物生理学会第57回年会, Mar. 2016, Japanese, 盛岡, Domestic conference

  Oral presentation


• Day length- and light quality-dependent expression of BONOBO, a master regulatory gene for growth-phase transition in the liverwort Marchantia polymorpha.

  Shohei Yamaoka, Keisuke Inoue, Hirokazu Tomogane, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Crucial roles of the ROS-signaling enzymes, Rbohs, in development and morphogenesis of Marchantia polymorpha.

  Kenji Hashimoto, Takashi Kimura, Shoji Yabuta, Toru Yamada, Hidetaka Kaya, Nobutaka Kitahata, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Kazuyuki Kuchitsu

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Conserved mechanism for secondary meristem formation in land plants

  ISHIZAKI Kimitsune

  第57回日本植物生理学会年会, Mar. 2016, English, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  [Invited]

  Nominated symposium


• Characterization of MpROS1X, an X-chromosomal homolog of REPRESSOR OF SILENCING 1, in the liverwort Marchantia polymorpha

  Taisuke Togawa, Daijiro Harada, Shigeyuki Tsukamoto, Kimitsune Ishizaki, Masaki Niwa, Takashi Araki, Katsushi Yamaguchi, Shuji Shigenobu, Takayuki Kohchi, Katsuyuki T. Yamato

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• Characterization of KARAPPO, an essential gene for gemma formation in Marchantia polymorpha.

  Takuma Hiwatashi, Katsushi Yamaguchi, Shuji Shigenobu, Shinichiro Sawa, Hiroyuki Kirita, FUKAKI Hidehiro, MIMURA TETSURO, Takayuki Kohchi, ISHIZAKI Kimitsune

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Poster presentation


• A NIMA-related kinase regulates directional tip growth of rhizoid cells in Marchantia polymorpha

  Kento Otani, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Taku Takahashi, Hiroyasu Motose

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• Analysis of temperature-sensing mechanism in Saintpaulia

  Yuuki Narita, Miwa Ohnishi, Noriaki Kadohama, Yoshihiro Suzuki, Hidetoshi Iida, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

  （一社）日本植物生理学会第57回年会, Mar. 2016, Japanese, 盛岡, Domestic conference

  Poster presentation


• Analysis of seasonal re-translocation of phosphate in a deciduous woody plant, Populus alba

  Yuko Kurita, Kei’ichi Baba, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Keitaro Tanoi, Yasuko Kaneko, Tomoko M. Nakanishi, Tetsuro Mimura

  （一社）日本植物生理学会第57回年会, Mar. 2016, Japanese, 盛岡, Domestic conference

  Oral presentation


• Analysis of downstream factors of phototropin in blue-light-dependent dorsoventral patterning of Marchantia polymorpha.

  Aino Komatsu, Yukiko Ashihara, Mika Terai, Noriyuki Suetsugu, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi

  第57回日本植物生理学会年会, Mar. 2016, Japanese, 日本植物生理学会, 岩手大学上田キャンパス, Domestic conference

  Oral presentation


• 植物における栄養繁殖の分子メカニズムとその進化

  ISHIZAKI KIMITSUNE

  「性と成熟：その普遍性と多様性を支える機構」, Feb. 2016, Japanese, サントリーワールドリサーチセンター（ＳＷＲ）メインホール, Domestic conference

  [Invited]

  Invited oral presentation


• 基部植物ゼニゴケから考える植物の繁殖戦略

  ISHIZAKI KIMITSUNE

  第35回インターゲノミクスセミナー「植物の環境適応戦略：進化の過程で獲得した巧妙なメカニズム」, Jan. 2016, Japanese, 神戸大学大学院 農学部Ｂ101, Domestic conference

  [Invited]

  Invited oral presentation


• Artificial modification of four seasons response in plants

  MIMURA TETSURO, 栗田 悠子, 真鍋 瞬, 岩村 青子, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, OHNISHI MIWA, 馬場 啓一

  第22回時間生物学会, Nov. 2015, Japanese, 東京大学, Domestic conference

  Oral presentation


• An ancient regulatory mechanism for secondary meristem formation in land plants

  ISHIZAKI KIMITSUNE

  "Towards Increased Plant Productivity through Understanding of Environmental Responses and Epigenetic Regulation", Nov. 2015, English, 理化学研究所（横浜キャンパス）, International conference

  [Invited]

  Invited oral presentation


• 無性芽形成異常変異体karappo-2における変異原因遺伝子の解析

  樋渡 琢真, 山口 勝司, 重信 秀治, 澤 進一郎, FUKAKI HIDEHIRO, MIMURA TETSURO, 河内 孝之, ISHIZAKI KIMITSUNE

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• 地表で暮らす葉状性苔類がとる光生存戦略

  西浜 竜一, 井上 佳祐, ISHIZAKI KIMITSUNE, 河内 孝之

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• 苔類ゼニゴケにおけるmicroRNAの機能解析

  都筑 正行, 藤本 剛史, 西浜 竜一, ISHIZAKI KIMITSUNE, 栗原 志夫, 松井 南, 河内 孝之, 濱田 隆宏, 渡邊 雄一郎

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• 植物組織における低分子量物質分布の質量顕微鏡による可視化

  MIMURA TETSURO, 姉川 彩, OHNISHI MIWA, 山本 浩太郎, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 高橋 勝利

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  OHNISHI MIWA, 成田 裕貴, 角浜 憲明, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 鈴木 祥弘, MIMURA TETSURO

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Poster presentation


• ランタナの花色変化における生理課程解析

  桑原 大季, 山本 浩太郎, OHNISHI MIWA, 七條 千津子, 水野 初, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 升島 努, MIMURA TETSURO

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ポプラにおけるリン酸転流経路の季節変化（ポスター）

  岩村 青子, 栗田 悠子, 馬場 啓一, OHNISHI MIWA, KOSUGE KEIKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Poster presentation


• ポプラにおけるリン酸転流経路の季節変化

  栗田 悠子, 馬場 啓一, 菅野 里美, 杉田 亮平, 廣瀬 農, OHNISHI MIWA, KOSUGE KEIKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 田野井 慶太朗, 金子 康子, 中西 友子, MIMURA TETSURO

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ニチニチソウ組織におけるTerpenoid indole alkaloidの合成と蓄積機構の解明

  山本 浩太郎, OHNISHI MIWA, 姉川 彩, 高橋 勝利, 水野 初, MURAKAMI AKIO, ISHIZAKI KIMITSUNE, 山崎 真巳, FUKAKI HIDEHIRO, 升島 努, MIMURA TETSURO

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケ精子超低温保存法の開発

  十川 太輔, 安達 徹, 田中 大介, ISHIZAKI KIMITSUNE, 河内 孝之, 大和 勝幸

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケ初期胚発生を制御する因子の探索

  丹羽 優喜, 酒井 友希, 石田 喬志, 西浜 竜一, ISHIZAKI KIMITSUNE, 大和 勝幸, 澤 進一郎, 河内 孝之, 荒木 崇

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケの杯状体形成を制御するGCAM1の機能解析

  塚本 成幸, 菅谷 友美, 大和 勝幸, 西浜 竜一, 久保 浩義, FUKAKI HIDEHIRO, MIMURA TETSURO, 河内 孝之, ISHIZAKI KIMITSUNE

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Poster presentation


• ゼニゴケの杯状体と生殖器の形成に関与するR2R3-MYB型転写因子GCAM2の解析

  高見 英幸, 塚本 成幸, 増田 晃秀, FUKAKI HIDEHIRO, MIMURA TETSURO, 河内 孝之, ISHIZAKI KIMITSUNE

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケの暗誘導老化Ⅱ.変異体sbr1の表現型の解析

  石井 優太, 山田 如都実, 佐藤 友哉, 児玉 豊, 山岡 尚平, ISHIZAKI KIMITSUNE, 河内 孝之, 井上 悠子, 森安 裕二

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Poster presentation


• ゼニゴケのSNARE分子から観る膜融合装置の保存性と多様性

  金澤 建彦, 恵良 厚子, 南野 尚紀, 鹿野 悠, 藤本 優, 植村 知博, 西浜 竜一, 大和 勝幸, ISHIZAKI KIMITSUNE, 西山 智明, 河内 孝之, 中野 明彦, 上田 貴志

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケのR2R3-MYB遺伝子過剰発現体におけるフェノール性二次代謝関連遺伝子の解析

  久保 浩義, ISHIZAKI KIMITSUNE, 野澤 俊介, 河内 孝之

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Poster presentation


• ゼニゴケNIMA関連キナーゼは仮根細胞の伸長を制御する

  大谷 健人, 高谷 彰吾, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之, 高橋 卓, 本瀬 宏康

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケMarchantia polymorphaの活性酸素種生成酵素MpRbohA, Bの発現部位と生理機能の解析

  木村 貴史, 橋本 研志, 籔田 渉二, 賀屋 秀隆, 北畑 信隆, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之, 朽津 和幸

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• ゼニゴケALOGドメイン遺伝子MpTAW1の機能解析

  経塚 淳子, ISHIZAKI KIMITSUNE, 嶋村 正樹, 徳永 浩樹, 吉田 明希子, 塚本 成幸, 高見 英幸, 西浜 竜一, 河内 孝之

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• シロイヌナズナ側根形成に関わるTOLS2ペプチドによるPUCHI遺伝子の発現制御機構の解析

  青木 優佳, 豊倉 浩一, 篠田 明徳, 郷 達明, ISHIZAKI KIMITSUNE, MIMURA TETSURO, FUKAKI HIDEHIRO

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• 基部陸上植物ゼニゴケのUV‐B受容体の機能解析

  森戸健, 宮城祐太, 藤平健太, 西浜竜一, 石崎公庸, 河内孝之, 近藤陽一

  日本植物生理学会年会要旨集, Mar. 2015, Japanese


• The transcription factor BONOBO regulates sexual organ development in the liverwort Marchantia polymorpha.

  Shohei Yamaoka, Hirokazu Tomogane, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi

  The 2nd International Symposium on Plant Environmental Sensing, Mar. 2015, English


• Isolation of a mutant showing accelerated senescence in darkness in Marchantia polymorpha L.

  Yuya Sato, Yutaka Kodama, Shohei Yamaoka, Kimitsune Ishizaki, Takayuki Kohchi, Yuko Inoue, Yuji Moriyasu

  第56回日本植物生理学会年会, Mar. 2015, Japanese


• 落葉性木本植物のリン酸分配・転流機構の解析

  栗田 悠子, 馬場 啓一, 菅野 里美, 杉田 亮平, 廣瀬 農, OHNISHI MIWA, ANEGAWA AYA, KOSUGE KEIKO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 田野井 慶太朗, 金子 康子, 中西 友子, MIMURA TETSURO

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 木本植物の心材形成時における栄養塩回収機構の解析

  真鍋 瞬, 栗田 悠子, 馬場 啓一, OHNISHI MIWA, KOSUGE KEIKO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 無性芽形成異常変異体karappo2 における変異原因遺伝子の同定

  樋渡 琢真, 山口 勝司, 重信 秀治, 澤 進一郎, SHICHIJO CHIZUKO, FUKAKI HIDEHIRO, MIMURA TETSURO, 河内 孝之, ISHIZAKI KIMITSUNE

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• 転写因子BONOBOは苔類ゼニゴケの有性生殖器官形成を制御する

  山岡 尚平, 友金 寛和, 西浜 竜一, 山口 勝司, 重信 秀治, ISHIZAKI KIMITSUNE, 大和 勝幸, 河内 孝之

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 苔類ゼニゴケを用いた転写因子PIFによる赤色光シグナル伝達機構の解析

  井上 佳祐, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 苔類ゼニゴケの雄性配偶子形成過程を制御する分子機構の解析

  肥後 あすか, 丹羽 優喜, 大和 勝幸, 坂本 智昭, 倉田 哲也, 白川 一, 重信 秀治, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之, 荒木 崇

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 苔類ゼニゴケにおけるオーキシン受容メカニズムの解析

  加藤 大貴, 鈴木 秀政, 山岡 尚平, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 苔類ゼニゴケにおけるmicroRNAの機能解析

  都筑 正行, 藤本 剛史, 西浜 竜一, ISHIZAKI KIMITSUNE, 栗原 志夫, 松井 南, 河内 孝之, 濱田 隆宏, 渡邊 雄一郎

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 植物細胞リン酸輸送機構とその進化について

  藤原 ひとみ, OHNISHI MIWA, SAKAYAMA HIDETOSHI, ISHIZAKI KIMITSUNE, 豊倉 浩一, 郷 達明, 関本 弘之, 西山 智明, SHICHIJO CHIZUKO, KOSUGE KEIKO, FUKAKI HIDEHIRO, MIMURA TETSURO

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 質量分析装置を用いたシロイヌナズナ植物体のオーキシン添加に応答する代謝変動解析

  姉川 彩, OHNISHI MIWA, 高橋 勝利, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  OHNISHI MIWA, 角浜 憲明, 鈴木 祥弘, 梶山 智晴, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 飯田 秀利, 神原 秀記, MIMURA TETSURO

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケのUV-B受容体の機能解析

  森戸 健, 宮城 祐太, 藤平 健太, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之, 近藤 陽一

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 基部陸上植物ゼニゴケにおける器官発生制御機構の解析

  ISHIZAKI KIMITSUNE

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  [Invited]

  Invited oral presentation


• 基部陸上植物ゼニゴケにおけるRAB GTPaseの網羅的解析

  南野 尚紀, 金澤 建彦, 恵良 厚子, 西浜 竜一, 大和 勝幸, ISHIZAKI KIMITSUNE, 西山 智明, 河内 孝之, 中野 明彦, 上田 貴志

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• ニチニチソウ茎組織におけるTerpenoid indole alkaloidの分布解析

  山本 浩太郎, OHNISHI MIWA, 姉川 彩, 高橋 勝利, 水野 初, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, 山崎 真巳, FUKAKI HIDEHIRO, 升島 努, MIMURA TETSURO

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• ゼニゴケ精子凍結保存法の開発

  十川 太輔, 安達 徹, 田中 大介, ISHIZAKI KIMITSUNE, 河内 孝之, 大和 勝幸

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• ゼニゴケを用いたミトコンドリア分裂因子の解析

  長岡 渚, 栗栖 里奈, 片山 健太, ISHIZAKI KIMITSUNE, 河内 孝之, 堤 伸浩, 有村 慎一

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• ゼニゴケの生殖におけるRWP-RK ファミリー遺伝子MpRKD の機能

  厚井 聡, 久永 哲也, 嶋村 正樹, ISHIZAKI KIMITSUNE, 河内 孝之, 中島 敬二

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• ゼニゴケのオーキシン応答転写因子ARF1は無性芽の細胞分裂パターンを制御する

  神埜 勝, 加藤 大貴, 武田 真由子, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• ゼニゴケにおける杯状体形成関連遺伝子GEMMA-CUP ASSOCIATED MYB 2 の解析

  高見 英幸, 塚本 成幸, 増田 晃秀, SHICHIJO CHIZUKO, FUKAKI HIDEHIRO, MIMURA TETSURO, 河内 孝之, ISHIZAKI KIMITSUNE

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• ゼニゴケにおける転写因子ABI3の機能解析

  兼井 雅和, 小野 大輔, 萩原 優羽, 小松 憲治, 竹澤 大輔, ISHIZAKI KIMITSUNE, 河内 孝之, 太治 輝昭, 林 隆久, 坂田 洋一

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• ゼニゴケゲノムアノテーションデータベースの構築

  長崎 英樹, ISHIZAKI KIMITSUNE, 大和 勝幸, 河内 孝之, 中村 保一

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• ゼニゴケNIMA関連キナーゼの機能解析

  竹田 健人, ISHIZAKI KIMITSUNE, 河内 孝之, 本瀬 宏康

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• ゼニゴケEILがエチレンおよび硫黄栄養応答に果たす役割

  牧野 宏美, 前田 祐華, 上土井 優貴, 陶山 明子, ISHIZAKI KIMITSUNE, 石田 咲子, 西浜 竜一, 河内 孝之, 平山 隆志, 丸山 明子

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• Vegetative propagation: development of asexual progenies from vegetative tissue

  Kimitsune Ishizaki

  第56回日本植物生理学会年会, Mar. 2015, English, 東京農業大学, Vegetative propagation is a form of asexual reproduction in plants. New individuals with functional meristems arise without fertilization and develop directly from vegetative tissues, such as leaves, stems, and roots. Many plant species from bryophytes to angiosperms have ability to propagate through vegetative propagation. Vegetative propagation is considered to be important f, Domestic conference

  Public symposium


• Systematic analysis of SNAREs inthe liverwort, Marchantiapolymorpha

  Takehiko Kanazawa, Atsuko Era, Naoki Minamino, Yu Shikano, Masaru Fujimoto, Ryuichi Nishihama, Katsuyuki Yamato, Kimitsune Ishizaki, Tomoaki Nishiyama, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda

  第56回日本植物生理学会年会, Mar. 2015, English, 東京農業大学, Domestic conference

  Oral presentation


• Search for the regulators of earlysporophyte development inMarchantia polymorpha

  Masaki Niwa, Yuuki Sakai, Asuka Higo, Motomu Endo, Ayako Yamaguchi, Kimitsune Ishizaki, Katsuyuki T. Yamato, Ryuichi Nishihama, Takashi Ishida, Shinichiro Sawa, Takayuki Kohchi, Takashi Araki

  第56回日本植物生理学会年会, Mar. 2015, English, 東京農業大学, Domestic conference

  Oral presentation


• R2R3-MYB型転写因子GCAM1は苔類ゼニゴケの杯状体形成を制御する

  塚本 成幸, 菅谷 友美, 大和 勝幸, 西浜 竜一, 久保 浩義, SHICHIJO CHIZUKO, FUKAKI HIDEHIRO, MIMURA TETSURO, 河内 孝之, ISHIZAKI KIMITSUNE

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference

  Poster presentation


• POSITIVE AND NEGATIVE REGULATORY MECHANISMS OF ABA SIGNALING ARE CRUCIAL FOR GROWTH AND STRESS

  Totan Kumar Ghosh, Shuuhei Murai, Keito Kato, Kimitsune Ishizaki, Takayuki Kohchi, Yoichi Sakata, DaisukeTakezawa

  第56回日本植物生理学会年会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

  Poster presentation


• Phosphate status in plant life

  Yuko Kurita, Kei’ichi Baba, Satomi Kanno, Miwa Ohnishi, Keiko Kosuge, Aya Anegawa, Kazuya Okada, Youichi Masuda, Taro Suzuki, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

  The 2nd International Symposium on Plant Environmental Sensing, Mar. 2015, English, AIST, Odaiba, International conference

  Poster presentation


• Mechanism of cell injury induced by a rapid temperature decrease in Saintpaulia sp. leaves.

  Miwa Ohnishi, Noriaki Kadohama, Yoshihiro Suzuki, Tomoharu Kajiyama, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Hidetoshi Iida, Hideki Kambara, Tetsuro Mimura

  The 2nd International Symposium on Plant Environmental Sensing, Mar. 2015, English, AIST, Odaiba, International conference

  Poster presentation


• Isolation of a mutant showingaccelerated senescence in darknessin Marchantia polymorpha L.

  Yuya Sato, Yutaka Kodama, Shohei Yamaoka, Kimitsune Ishizaki, Takayuki Kohchi, Yuko Inoue, Yuji Moriyasu

  第56回日本植物生理学会年会, Mar. 2015, English, 東京農業大学, Domestic conference

  Oral presentation


• Evolutionary analysis of phosphate transport mechanisms in plant cells

  Hitomi Fujiwara, Miwa Ohnishi, Hidetoshi Sakayama, Kimitsune Ishizaki, Koichi Toyokura, Tatsuaki Goh, Hiroyuki Sekimoto, Tomoaki Nishiyama, Chizuko Shichijo, Keiko Kosuge, Hidehiro Fukaki, Tetsuro Mimura

  The 2nd International Symposium on Plant Environmental Sensing, Mar. 2015, English, AIST, Odaiba, International conference

  Poster presentation


• Cell-specific localization of terpenoid indole alkaloids revealed by new metabolome analyses

  Kotaro Yamamoto, Miwa Ohnishi, Aya Anegawa, Katsutoshi Takahashi, Hajime Mizuno, Chizuko Shichijo, Kimitsune Ishizaki, Mami Yamazaki, Hidehiro Fukaki, Tsutomu Masujima, Tetsuro Mimura

  The 2nd International Symposium on Plant Environmental Sensing, Mar. 2015, English, AIST, Odaiba, International conference

  Poster presentation


• 基部陸上植物ゼニゴケのUV‐B耐性におけるカルコンシンターゼ(MpCHS)の役割

  藤平健太, 西浜竜一, 森山亜沙美, 石崎公庸, 森戸健, 河内孝之, 宮城祐太, 近藤陽一

  関東学院大学理工/建築・環境学会研究発表講演論文集, 2015, Japanese


• 基部陸上植物ゼニゴケのUV‐B受容体の機能解析

  森戸健, 西浜竜一, 宮内渉, 石崎公庸, 藤平健太, 河内孝之, 宮城祐太, 近藤陽一

  関東学院大学理工/建築・環境学会研究発表講演論文集, 2015, Japanese


• 植物の有用遺伝子を探索する新たなシステムの確立

  寺澤拓弥, 西浜竜一, 小林美由希, 石崎公庸, 二瓶遼太, 河内孝之, 宮城祐太, 近藤陽一

  関東学院大学理工/建築・環境学会研究発表講演論文集, 2015, Japanese


• 植物NADPH oxidaseの分子進化と,基部陸上植物ゼニゴケに探る活性制御の基本機構

  橋本研志, 山田融, 船木洋一, 賀屋秀隆, 賀屋秀隆, 北畑信隆, 石崎公庸, 西浜竜一, 河内孝之, 朽津和幸, 朽津和幸

  日本生化学会大会(Web), 2015, Japanese


• 基部陸上植物ゼニゴケのNADPH oxidase遺伝子MpRbohA,Bの発現部位と機能の解析

  木村貴史, 橋本研志, 籔田渉二, 賀屋秀隆, 北畑信隆, 石崎公庸, 西浜竜一, 河内孝之, 朽津和幸, 朽津和幸

  日本生化学会大会(Web), 2015, Japanese


• The transcription factor BONOBO appears to regulate gametangiophore formation in Marchantia polymorpha.

  Shohei Yamaoka, Kimitsune Ishizaki, Katsuyuki T. Yamato, Ryuichi Nishihama, Takayuki Kohchi

  Marchantia Workshop 2014, Dec. 2014, English

  [Invited]


• The role of the R2R3-MYB transcription factor, GCAM2, in the development of gemma cup in Marchantia polymorpha.

  Hideyuki Takami, Shigeyuki Tsukamoto, Akihide Masuda, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, Kimitsune Ishizaki

  Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, The liverwort Marchantia polymorpha forms cupule-like structures, gemma cups, on the dorsal side of thallus, where dozens of clonal progenies, gemmae, are generated. We isolated a mutant of M. polymorpha defective in gemma cup development from T-DNA-tagged lines. The T-DNA was inserted into a 5’UTR of a gene encoding an R2R3-MYB transcription factor. Analysis of the promoter ac, International conference

  Poster presentation


• Molecular characterization of karappo2, a gemma-less mutant, in Marchantia polymorpha.

  Takuma Hiwatashi, Katsushi Yamaguchi, Shuji Shigenobu, Shinichiro Sawa, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, ISHIZAKI KIMITSUNE

  Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, Gemma is a clonal progeny developed from the base of gemma-cup in Marchantia polymorpha. To investigate the regulatory mechanism for gemma development, we focused on a mutant, karappo2 (kar2), which was originally isolated from transgenics obtained by particle bombardment protocol. There is no gemma initial observed at the base of gemma-cup in kar2, while gemma-cups are regular, International conference

  Poster presentation


• GEMMA CUP-ASSOCIATED MYB1 is essential for the development of gemma-cup in the liverwort Marchantia polymorpha L.

  Shigeyuki Tsukamoto, Tomomi Sugatani, Katsuyuki T. Yamato, Katsushi Yamaguchi, Shuji Shigenobu, Hiroyoshi Kubo, Hidehiro Fukaki, Tetsuro Mimura, Takayuki Kohchi, Kimitsune Ishizaki

  Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, Marchantia polymorpha has an ability to propagate asexually via gemmae generated in gemma-cup formed on the dorsal side of thallus. To investigate genes involved in the process of gemma and gemma-cup development, we performed RNA-seq analysis comparing gemma-cups contaning gemmae and thallus without any gemma-cup. Through the comprehensive transcriptome analysis, we identified, International conference

  Poster presentation


• Evolutional analysis of phosphate trasnport mechanism in plant cells

  Fujiwara Hitomi, Ohnishi Miwa, Sakayama Hidetoshi, Ishizaki Kimitsune, Toyokura Koichi, Sekimoto Hiroyuki, Nishiyama Tomoaki, Shichijo Chizuko, Kosuge Keiko, Fukaki Hidehiro, MIMURA TETSURO

  Marchantia Workshop 2014, Dec. 2014, English, Kobe University, International conference

  Poster presentation


• Development of gemma and gemma-cup in Marchantia polymorpha

  Kimitsune Ishizaki

  Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, Many species in bryophytes produce gemmae on their gametophytes as a means of vegatative propagation. In the liverwort, Marchantia polymorpha, each gemma is originated from a single epidermal cell at the bottom of the gemma-cup, developed into a mature gemma through a number of cell divisions, and detached finally from the parental thallus by undergoing cell death of the stalk, International conference

  [Invited]

  Invited oral presentation


• An overview of available tools for Marchantia polymorpha

  Kimitsune Ishizaki

  Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, The liverwort Marchantia polymorpha L. has become a fascinating model organism for plant biology, as its molecular genetic resources have been being developed. Here I am going to introduce the techniques and resources for Marchantia research, especially focusing on transformation, Gateway-compatible binary vector system, homologous recombination mediated gene targeting, and EMS, International conference

  [Invited]

  Invited oral presentation


• 苔類ゼニゴケにおけるオーキシン信号伝達因子ARFの機能分化

  加藤大貴, 神埜勝, 白川一, 石崎公庸, 西浜竜一, 河内孝之

  植物化学調節学会, Oct. 2014


• オーキシン応答転写因子ARF1による無性芽発生の制御

  神埜勝, 加藤大貴, 武田真由子, 石崎公庸, 西浜竜一, 河内孝之

  植物化学調節学会, Oct. 2014


• オーキシン応答転移因子ARF1による無性芽発生の制御

  神埜勝, 加藤大貴, 武田真由子, 石崎公庸, 西浜竜一郎, 河内孝之

  植物の生長調節, Oct. 2014, Japanese


• ゼニゴゲの接合子におけるMpLFYの機能解析

  丹羽優喜, 酒井友希, 肥後あすか, 遠藤求, 山口礼子, 石崎公庸, 大和勝幸, 西浜竜一, 河内孝之, 荒木崇

  日本植物学会大会研究発表記録, Sep. 2014, Japanese


• 基部陸上植物ゼニゴケにおけるGI‐FKF複合体を介した光周性生長相制御機構

  久保田茜, 喜多祥吾, 永山啓太郎, 山岡尚平, 西浜竜一, 石崎公庸, 河内孝之

  日本植物学会大会研究発表記録, Sep. 2014, Japanese


• 落葉性木本植物のリン酸分配・転流機構の解析

  栗田 悠子, 馬場 啓一, OHNISHI MIWA, ANEGAWA AYA, KOSUGE KEIKO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• 木本植物におけるイオン輸送機構の解析

  真鍋 瞬, 栗田 悠子, 馬場 啓一, OHNISHI MIWA, KOSUGE KEIKO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケの造精器および精子の発生過程に関与する遺伝子発現プログラムを制御する機構の解析

  肥後 あすか, 丹羽 優喜, 遠藤 求, ISHIZAKI KIMITSUNE, 大和 勝幸, 西浜 竜一, 河内 孝之, 荒木 崇

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおける細胞膜H⁺-ATPaseの機能解析

  楊 為雄, 奥村 将樹, 井上 晋一郎, 高橋 宏二, ISHIZAKI KIMITSUNE, 河内 孝之, 木下 俊則

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおけるSBP型転写因子の機能解析

  大神 貴史, 山口 礼子, 丹羽 優喜, 遠藤 求, ISHIZAKI KIMITSUNE, 河内 孝之, 荒木 崇

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおいてCYCLING DOF FACTORは光周性成長相転換を抑制する

  永山 啓太郎, 久保田 茜, 喜多 祥吾, 山岡 尚平, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• 植物細胞リン酸輸送機構とその進化について

  藤原 ひとみ, OHNISHI MIWA, SAKAYAMA HIDETOSHI, ISHIZAKI KIMITSUNE, 豊倉 浩一, 関本 弘之, 西山 智明, SHICHIJO CHIZUKO, KOSUGE KEIKO, FUKAKI HIDEHIRO, MIMURA TETSURO

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• 植物ROS生成酵素の活性制御の基本機構をゼニゴケに探る

  橋本 研志, 藪田 渉二, 山田 融, 賀屋 秀隆, 北畑 信隆, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之, 朽津 和幸

  日本植物学会第78回大会, Sep. 2014, English, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  OHNISHI MIWA, 角浜 憲明, 高橋 勝利, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 鈴木 祥弘, MIMURA TETSURO

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケにおける膜交通因子の網羅的解析

  南野 尚紀, 金澤 建彦, 恵良 厚子, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之, 中野 明彦, 上田 貴志

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケにおけるGI-FKF複合体を介した光周性生長相制御機構

  久保田 茜, 喜多 祥吾, 永山 啓太郎, 山岡 尚平, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケ葉状体の暗誘導老化に関する突然変異体の単離と解析

  佐藤 友哉, 児玉 豊, ISHIZAKI KIMITSUNE, 河内 孝之, 井上 悠子, 森安 裕二

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• ゼニゴケ無性芽の－80℃フリーザーでの長期保存法の開発

  田中 大介, 大和 勝幸, ISHIZAKI KIMITSUNE, 河内 孝之

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• ゼニゴケの接合子におけるMpLFYの機能解析

  丹羽 優喜, 酒井 友希, 肥後 あすか, 遠藤 求, 山口 礼子, ISHIZAKI KIMITSUNE, 大和 勝幸, 西浜 竜一, 河内 孝之, 荒木 崇

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケの形態形成におけるmicroRNAの機能解析

  都筑 正行, 藤本 剛史, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之, 濱田 隆宏, 渡邊 雄一郎

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケの栄養繁殖器官で発現するR2R3型MYB遺伝子の単離と機能解析

  塚本 成幸, 菅谷 友美, 大和 勝幸, 山口 勝司, 重信 秀治, 西浜 竜一, SHICHIJO CHIZUKO, 久保 浩義, 河内 孝之, FUKAKI HIDEHIRO, MIMURA TETSURO, ISHIZAKI KIMITSUNE

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケのオーキシン応答転写因子ARF1は無性芽の発生を制御する

  神埜 勝, 加藤 大貴, 武田 真由子, ISHIZAKI KIMITSUNE

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• ゼニゴケから見えてきた栄養繁殖と腋芽発生の共通制御メカニズム

  ISHIZAKI KIMITSUNE

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• ゼニゴケCLEペプチドホルモンの機能

  平川 有宇樹, 田畑 亮, ISHIZAKI KIMITSUNE, 河内 孝之, John Bowman, 澤 進一郎

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• シロイヌナズナ側根形成におけるLBDの下流遺伝子TOLS2の解析

  篠田 明徳, 郷 達明, 豊倉 浩一, 上原 健生, 奥島 葉子, 田坂 昌生, ISHIZAKI KIMITSUNE, MIMURA TETSURO, FUKAKI HIDEHIRO

  日本植物学会第78回大会, Sep. 2014, Japanese, 明治大学 生田キャンパス, Domestic conference

  Oral presentation


• シロイヌナズナにおける葉の維管束鞘細胞の形成・分化機構の解析

  山本 真佑子, 豊倉 浩一, 郷 達明, ISHIZAKI KIMITSUNE, MIMURA TETSURO, FUKAKI HIDEHIRO

  日本植物学会第78回大会, Sep. 2014, English, 明治大学 生田キャンパス, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケにおける祖先的な赤色光シグナル伝達機構

  井上佳祐, 石崎公庸, 西浜竜一, 河内孝之

  日本光生物学協会, Aug. 2014


• ゼニゴケフォトトロピンMpphotの生化学的解析と下流因子の探索

  寺井三佳, 小松愛乃, 末次憲之, 四井いずみ, 中神弘史, 石田咲子, 石崎公庸, 西浜竜一, 河内孝之

  日本光生物学協会, Aug. 2014


• コケ植物配偶体における器官発生の分子遺伝学

  ISHIZAKI KIMITSUNE

  イネ遺伝学・分子生物学ワークショップ ２０１４, Jul. 2014, Japanese, 東京大学弥生講堂一条ホール, Domestic conference

  [Invited]

  Invited oral presentation


• 基部陸上植物ゼニゴケを用いた光周性花成制御因子GI‐FKF1複合体の祖先的機能の解析

  久保田茜, 喜多祥吾, 石崎公庸, 西浜竜一, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2014, Japanese


• 基部陸上植物ゼニゴケのUV‐B応答機構の解析

  宮城祐太, 平口尚樹, 伊藤考志, 植村泰将, 武田圭祐, 海老澤政彦, 山沖美穂, 菅間俊, 森戸健, 西浜竜一, 石崎公庸, 河内孝之, 近藤陽一

  日本植物生理学会年会要旨集, Mar. 2014, Japanese


• 苔類ゼニゴケを用いた祖先的な赤色光シグナル伝達機構の解析

  井上佳祐, 西浜竜一, 石崎公庸, 河内孝之

  日本農芸化学会大会講演要旨集(Web), Mar. 2014, Japanese


• 落葉性木本植物のリン酸分配・転流機構の解析

  栗田 悠子, 馬場 啓一, OHNISHI MIWA, ANEGAWA AYA, KOSUGE KEIKO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山, Domestic conference

  Oral presentation


• 転写因子の相互作用によるゼニゴケのオーキシン信号伝達ネットワーク

  加藤 大貴, 神埜 勝, 白川 一, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおける転写因子レパートリー

  白川 一, 井上 佳祐, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおける転写因子HY5を介した光形態形成制御機構

  竹谷 千尋, 井上 佳祐, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおける頂端細胞の選択を介した青色光依存的な背腹性決定機構

  芦原 悠紀子, 小松 愛乃, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおけるオキシリピン代謝酵素CYP74ファミリー酵素遺伝子の機能解析

  肥塚 崇男, ISHIZAKI KIMITSUNE, 河内 孝之, 松井 健二

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• 植物細胞リン酸輸送機構とその進化について

  藤原 ひとみ, OHNISHI MIWA, SAKAYAMA HIDETOSHI, ISHIZAKI KIMITSUNE, 関本 弘之, 西山 智明, SHICHIJO CHIZUKO, FUKAKI HIDEHIRO, MIMURA TETSURO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Oral presentation


• 質量分析装置を用いたシロイヌナズナ植物体のオーキシン添加に応答する代謝変動解析

  姉川 彩, OHNISHI MIWA, 高橋 勝利, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山, Domestic conference

  Poster presentation


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  OHNISHI MIWA, 角浜 憲明, 高橋 大輔, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 上村 松生, 鈴木 祥弘, MIMURA TETSURO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケを用いた光周性花成制御因子GI-FKF1複合体の祖先的機能の解析

  久保田 茜, 喜多 祥吾, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• 基部陸上植物ゼニゴケのUV-B応答機構の解析

  宮城 祐太, 平口 尚樹, 伊藤 考志, 植村 泰将, 武田 圭祐, 海老澤 政彦, 山沖 美穂, 菅間 俊, 森戸 健, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之, 近藤 陽一

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• フタバネゼニゴケにおけるキチン防御応答遺伝子の発現解析

  宮本 綾乃, 古崎 利紀, 榊原 恵子, ISHIZAKI KIMITSUNE, 緒方 法親, 石井 一夫, 嶋村 正樹, 加来 華江, 河内 孝之, 渋谷 直人, 中川 知己

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• フィトクロムによるトマト芽生えのフック巻込みに関わる植物ホルモン

  香山 大輝, 周伊テイ, 高原 綾子, 姉川 彩, OHNISHI MIWA, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO, 橋本 徹, SHICHIJO CHIZUKO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Oral presentation


• ニチニチソウ組織におけるTerpenoid indole alkaloid合成機構の解明

  山本 浩太郎, OHNISHI MIWA, ANEGAWA AYA, 高橋 勝利, IWASAKI TETSUSHI, 水野 初, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, 山崎 真巳, FUKAKI HIDEHIRO, 升島 努, MIMURA TETSURO

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学・五福キャンパス, Domestic conference

  Poster presentation


• ゼニゴケ研究地平への投射：オーキシン信号伝達を例に

  河内 孝之, 加藤 大貴, 西浜 竜一, 大和 勝幸, ISHIZAKI KIMITSUNE

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• ゼニゴケ気室の発生初期段階ではNOPPERABO1のE3ユビキチンリガーゼ活性が必須である

  水谷 未耶, ISHIZAKI KIMITSUNE, 増田 晃秀, 嶋村 正樹, 西浜 竜一, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学・五福キャンパス, Domestic conference

  Poster presentation


• ゼニゴケを用いた有用遺伝子探索システムの構築

  近藤 陽一, 宮城 祐太, 生江 亮介, 久保寺 美登, 関川 萌美, 滝澤 達哉, 吉積 毅, 松井 南, ISHIZAKI KIMITSUNE, 河内 孝之

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• ゼニゴケMpLFYノックアウト株の表現型解析

  丹羽 優喜, 酒井 友希, 肥後 あすか, 遠藤 求, 山口 礼子, ISHIZAKI KIMITSUNE, 大和 勝幸, 西浜 竜一, 河内 孝之, 荒木 崇

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Poster presentation


• コケ植物における無性芽の発生と休眠

  ISHIZAKI KIMITSUNE

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学五福キャンパス, Domestic conference

  Oral presentation


• コケ植物ANGUSTIFORIAに関する比較解析

  服部 考郎, ISHIZAKI KIMITSUNE, 武智 克彰, 榊原 恵子, 高野 博嘉, 河内 孝之, 塚谷 裕一

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学・五福キャンパス, Domestic conference

  Poster presentation


• E３ユビキチンリガーゼが関与する植物の細胞間隙形成

  石崎 公庸, 河内 孝之

  生化学, 2014, Japanese


• 苔類ゼニゴケを用いた植物microRNAネットワークの解明

  都筑正行, 藤本剛史, 西浜竜一, 石崎公庸, 河内孝之, 濱田隆宏, 渡邊雄一郎

  日本分子生物学会年会プログラム・要旨集(Web), 2014, Japanese


• 活性酸素‐カルシウムシグナルネットワークによる植物の生殖・発生の制御

  朽津和幸, 橋本研志, 賀屋秀隆, 木村幸恵, 河原崎朋子, 籔田渉二, 北畑信隆, 石崎公庸, 西浜竜一, 河内孝之

  日本分子生物学会年会プログラム・要旨集(Web), 2014, Japanese


• Gemma and gemma-cup development in Marchantia polymorpha

  Kimitsune Ishizaki

  International Marchantia Workshop 2013, Dec. 2013, English, Buln Buln Cabins, Yictoria, Australia, International conference

  Oral presentation


• 基部陸上植物ゼニゴケにおける概日時計を介した成長相制御機構

  久保田茜, 喜多祥吾, 村中智明, 石崎公庸, 大和勝幸, 青木摂之, 小山時隆, 西浜竜一, 河内孝之

  時間生物学, Oct. 2013, Japanese


• E3リガーゼNOPPERABO1はゼニゴケ気室形成を正に制御する

  石崎公庸, 水谷未耶, 嶋村正樹, 増田晃秀, 西浜竜一, 河内孝之

  日本農芸化学会関西支部講演会講演要旨集, Sep. 2013, Japanese


• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム

  OHNISHI MIWA, 角浜 憲明, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 鈴木 祥弘, MIMURA TETSURO

  日本植物学会第77回大会, Sep. 2013, Japanese, 札幌, Domestic conference

  Poster presentation


• バラの花色決定における液胞機能の解析

  西本 朱里, OHNISHI MIWA, ANEGAWA AYA, 興津 奈央子, 高橋 勝利, MURAKAMI AKIO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, 田中 良和, MIMURA TETSURO

  日本植物学会第77回大会, Sep. 2013, Japanese, 札幌, Domestic conference

  Poster presentation


• ニチニチソウ葉組織の単一細胞種を用いた二次代謝機構の解析

  山本 浩太郎, OHNISHI MIWA, ANEGAWA AYA, 高橋 勝利, IWASAKI TETSUSHI, 水野 初, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, 山崎 真巳, FUKAKI HIDEHIRO, 升島 努, MIMURA TETSURO

  日本植物学会第77回大会, Sep. 2013, Japanese, 札幌, Domestic conference

  Oral presentation


• シロイヌナズナ植物体のオーキシン添加に応答する 代謝変動解析

  ANEGAWA AYA, OHNISHI MIWA, 高橋 勝利, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  日本植物学会第77回大会, Sep. 2013, Japanese, 札幌, Domestic conference

  Poster presentation


• 苔類ゼニゴケの活性酸素種生成酵素の機能解析

  籔田渉二, 山田融, 賀屋秀隆, 石崎公庸, 西浜竜一, 河内孝之, 朽津和幸

  日本植物学会大会研究発表記録, Aug. 2013, Japanese


• 苔類ゼニゴケにおけるAUX/IAA‐ARF相互作用を介したオーキシン転写制御機構の解析

  加藤大貴, 神埜勝, 白川一, 石崎公庸, 西浜竜一, 河内孝之

  日本植物学会大会研究発表記録, Aug. 2013, Japanese


• 植物の活性酸素種生成酵素の発現部位・活性制御機構の網羅的解析

  朽津和幸, 中内愛由子, 籔田渉二, 賀屋秀隆, 村上祐樹, 森恭一郎, 河原崎朋子, 石崎公庸, 西浜竜一, 河内孝之

  バイオイメージング, Aug. 2013, Japanese


• 苔類ゼニゴケにおけるGI,FKFを介した日長依存的な成長相制御機構

  喜多祥吾, 久保田茜, 石崎公庸, 西浜竜一, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• 苔類ゼニゴケの背腹性決定は青色光受容体フォトトロピンとオーキシン極性輸送によって制御される

  芦原悠紀子, 小松愛乃, 西浜竜一, 石崎公庸, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• 苔類ゼニゴケにおける青色光受容体フォトトロピンが関与する葉緑体光定位運動の解析

  小松愛乃, 芦原悠紀子, 坪井秀憲, 末次憲之, 石崎公庸, 西浜竜一, 和田正三, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• 苔類ゼニゴケにおけるHY5を介した光形態形成シグナル伝達経路の解析

  竹谷千尋, 井上佳祐, 石崎公庸, 西浜竜一, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• ゼニゴケの赤色光によるサイクリンD遺伝子発現制御機構

  西浜竜一, 真鍋諒, 中村衣里, 井上佳祐, 石崎公庸, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• NOPPERABO1は細胞膜に局在し,ゼニゴケ気室の発生初期段階を制御する

  水谷未耶, 石崎公庸, 増田晃秀, 嶋村正樹, 西浜竜一, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• 苔類ゼニゴケにおける転写因子PIFを介した赤色光シグナル伝達機構

  井上佳祐, 石崎公庸, 西浜竜一, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• 原核生物型PEBPファミリータンパク質CORのシロイヌナズナとゼニゴケにおける機能解析

  森花小百合, 竹本覚, 辻井由香, 有手友嗣, 竹村美保, 酒井友希, 山口礼子, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• ゼニゴケを用いたミトコンドリア分裂因子の解析

  長岡渚, 栗栖里奈, 片山健太, 石崎公庸, 河内孝之, 堤伸浩, 有村慎一

  日本植物生理学会年会要旨集, Mar. 2013, Japanese


• 落葉性木本植物のリン酸分配・転流機構の解析

  栗田 悠子, 馬場 啓一, OHNISHI MIWA, ANEGAWA AYA, KOSUGE KEIKO, SHICHIJO CHIZUKO, ISHIZAKI KIMITSUNE, FUKAKI HIDEHIRO, MIMURA TETSURO

  日本植物学会第77回大会, 2013, Japanese, 札幌, Domestic conference

  Oral presentation


• 苔類におけるアブシジン酸受容体の解析

  村井 周平, 梅澤 泰史, 篠崎 一雄, ISHIZAKI KIMITSUNE, 大和 勝幸, 西浜 竜一, 河内 孝之, 竹澤 大輔

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおける光依存的な細胞分裂活性制御機構

  西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• 苔類ゼニゴケにおけるAUX/IAA-ARF相互作用を介したオーキシン転写制御機構の解析

  加藤 大貴, 神埜 勝, 白川 一, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• 赤色光受容体フィトクロムと光合成によるゼニゴケ細胞周期の光制御

  真鍋 諒, 中村 衣里, 井上 佳祐, 保坂 将志, ISHIZAKI KIMITSUNE, 河内 孝之, 西浜 竜一

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• 光合成生物における生存前略の分子機構に関する研究

  ISHIZAKI KIMITSUNE

  日本農芸化学会関西支部第479回講演会, 2013, Japanese, 京都府立大学, Domestic conference

  Oral presentation


• 基部陸上植物ゼニゴケにおける転写因子PIFを介した赤色光シグナル伝達機構

  井上 佳祐, 西浜 竜一, ISHIZAKI KIMITSUNE, 河内 孝之

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• ゼニゴケ葉状体における暗誘導老化

  佐藤 友哉, 児玉 豊, ISHIZAKI KIMITSUNE, 河内 孝之, 井上 悠子, 森安 裕二

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• ゼニゴケを用いた植物特異的膜交通経路の研究

  恵良 厚子, 海老根 一生, ISHIZAKI KIMITSUNE, 白川 一, 西浜 竜一, 河内 孝之, 中野 明彦, 上田 貴志

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• ゼニゴケのオーキシン信号伝達因子MpARF1による無性芽発生制御

  神埜 勝, 加藤 大貴, 武田 真由子, ISHIZAKI KIMITSUNE, 西浜 竜一, 河内 孝之

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• ゼニゴケNOPPERABO1はE3ユビキチンリガーゼとして気室の細胞間隙形成を正に制御する

  水谷 未耶, ISHIZAKI KIMITSUNE, 増田 晃秀, 嶋村 正樹, 西浜 竜一, 河内 孝之

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Poster presentation


• シロイヌナズナ側根形成におけるLBD16の下流遺伝子TOLS2の解析

  篠田 明徳, 郷 達明, 豊倉 浩一, 上原 健生, 奥島 葉子, 田坂 昌生, ISHIZAKI KIMITSUNE, MIMURA TETSURO, FUKAKI HIDEHIRO

  日本植物学会第77回大会, 2013, Japanese, 北海道大学札幌キャンパス, Domestic conference

  Oral presentation


• ゼニゴケにおけるアレンオキシドシクラーゼの機能解析

  山本祐介, 石崎公庸, 河内孝之, 高橋知寛, 松浦英幸, 高橋公咲

  植物の生長調節, Oct. 2012, Japanese


• ゼニゴケ気室形成制御遺伝子の機能解析

  水谷未耶, 石崎公庸, 増田晃秀, 桐田啓如, 西浜竜一, 河内孝之

  日本植物学会大会研究発表記録, Sep. 2012, Japanese


• 苔類ゼニゴケ葉状体の背腹性はフォトトロピンを介して青色光依存的に決定される

  芦原悠紀子, 小松愛乃, 石崎公庸, 西浜竜一, 河内孝之

  日本植物学会大会研究発表記録, Sep. 2012, Japanese


• ゼニゴケのフィトクロムによる細胞周期制御機構

  西浜竜一, 中村衣里, 保坂将志, 井上佳祐, 真鍋諒, 石崎公庸, 河内孝之

  日本植物学会大会研究発表記録, Sep. 2012, Japanese


• メリステムにおけるDNA複製のイメージング解析

  林耕磨, 長谷川淳子, 豊岡博子, 西浜竜一, 金鍾明, 石崎公庸, 野崎久義, 河内孝之, 関原明, 松永幸大

  日本植物生理学会年会要旨集, Mar. 2012, Japanese


• 苔類ゼニゴケにおける赤色光による細胞周期制御機構

  中村衣里, 保坂将志, 井上佳祐, 石崎公庸, 西浜竜一, 河内孝之

  日本植物生理学会年会要旨集, Mar. 2012, Japanese


• 青色光受容体フォトトロピンは苔類ゼニゴケの葉状体背腹性決定を制御する

  芦原悠紀子, 小松愛乃, 石崎公庸, 西浜竜一, 河内孝之

  日本農芸化学会大会講演要旨集(Web), Mar. 2012, Japanese


• 赤色光は苔類ゼニゴケの細胞分裂を制御する

  井上佳祐, 中村衣里, 保坂将志, 石崎公庸, 西浜竜一, 河内孝之

  日本農芸化学会大会講演要旨集(Web), Mar. 2012, Japanese


• ゼニゴケ葉緑体RNAポリメラーゼシグマ因子(Mpsig1)変異体の解析から明らかとなった陸上植物シグマ因子の機能分化

  上田 実, 高見 常明, Peng Lianwei, 石崎 公庸, 河内 孝之, 鹿内 利治, 西村 芳樹

  日本分子生物学会、第35回年会、於 パシフィコ横浜、2011年12月, 2011, Japanese


• 苔類ゼニゴケにおけるPEBP family遺伝子の機能解析

  竹本覚, 辻井由香, 有手友嗣, 竹村美保, 酒井友希, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  日本植物学会大会研究発表記録, Sep. 2010, Japanese


• 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの機能解析

  酒井友希, 宮下結衣, 宇山和樹, 辻井由香, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  日本植物学会大会研究発表記録, Sep. 2010, Japanese


• ゼニゴケFLO/LFY相同遺伝子の機能解析

  宇山和樹, 宮下結衣, 辻井由香, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 福澤秀哉, 河内孝之, 荒木崇

  日本植物生理学会年会要旨集, Mar. 2010, Japanese


• 新規ゼニゴケ核ゲノム形質転換選抜用マーカーの開発

  上田 実, 石崎 公庸, 大和 勝幸, 河内 孝之, 鹿内 利治, 西村 芳樹

  日本植物生理学会、 第51回年会、於 熊本大学、2010年3月, 2010, Japanese


• Development of a novel selectable marker for liverwort (Marchantia polymorpha).

  Ueda M, Ishizaki K, Yamato K. T, Kohchi T, Shikanai T, Nishimura Y

  Marchantia workshop 2010, March 11-12, 2010, Kyoto, Japan, 2010, English


• ゼニゴケFLO/LFY相同遺伝子の機能解析

  宇山和樹, 辻井由香, 宮下結衣, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  日本植物学会大会研究発表記録, Sep. 2009, Japanese


• ゼニゴケFLO/LFY相同遺伝子の単離と解析

  辻井由香, 宇山和樹, 大門靖史, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  日本植物生理学会年会要旨集, Mar. 2009, Japanese


• The liverwort Marchantia polymorpha as an emerging model plant

  Katsuyuki T. Yamato, Kimitsune Ishizaki, Aino Komatsu, Akane Kubota

  Memorial Symposium for the 25th International Prize for Biology Celebrating Dr. Winslow R. Briggs, 2009, English


• ゼニゴケFLO/LFY相同遺伝子の単離と解析

  辻井由香, 大門靖史, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇

  日本植物学会大会研究発表記録, Sep. 2008, Japanese


• The critical role of Arabidopsis electron-transfer flavoprotein ubiquinone oxidereductase during dark induced starvation

  K Ishizaki, TA Larson, N Schauer, AR Fernie, IA Graham, CJ Leaver

  PLANT AND CELL PHYSIOLOGY, 2006, English, OXFORD UNIV PRESS


• Gene organization of the Y chromosome of the liverwort, Marchantia polymorpha

  K Oyama, KT Yamato, K Ishizaki, S Okada, M Fujisawa, S Nakayama, M Fujishita, T Bando, A Hasumi, R Sakata, T Nishio, K Yodoya, H Bando

  PLANT AND CELL PHYSIOLOGY, 2003, English, OXFORD UNIV PRESS


• Isolation of high-CO2 requiring mutants from Chlamydomonas reinhardtii by gene tagging.

  H Fukuzawa, K Ishizaki, S Matsueda, K Miura, T Inoue, K Ohyama

  PLANT PHYSIOLOGY, Jul. 1997, English, AMER SOC PLANT PHYSIOLOGISTS

• JAPAN SOCIETY FOR BIOSCIENCE


• THE BOTANICAL SOCIETY OF JAPAN


• THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

• 植物に存在するステロイドホルモンの進化的起源の解明

  野村 崇人, 石崎 公庸, 水谷 正治

  日本学術振興会, 科学研究費助成事業, 挑戦的研究(開拓), 宇都宮大学, 28 Jun. 2024 - 31 Mar. 2028


• コケ植物繁殖子の休眠を制御する分子メカニズムの解明

  ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (B), Apr. 2019 - Mar. 2022, Principal investigator

  Competitive research funding


• リプログラミングによる植物幹細胞の新生機構の解明

  Hayashi Makoto

  Ministry of Education, Culture, Sports, Science and Technology-Japan, Grant-in-Aid for Scientific Research on Innovative Areas, Sep. 2017 - Mar. 2021

  Competitive research funding


• 植物における栄養繁殖器官の発生開始を制御する分子メカニズム

  石崎 公庸

  科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2019, Principal investigator

  Competitive research funding


• Investigation on the evolution of the multicellular body plan in plants by focusing on comparative and functional genomics in charophycean algae

  Sakayama Hidetoshi, YAMADA Toshihiro, ISHIZAKI Kimitsune, NISHIYAMA Tomoaki, SUZUKI Yutaka, KAWAI Hiroshi, MIMURA Tetsuro, WATANABE Miyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2015 - 31 Mar. 2018

  In order to investigate the evolution of the multicellular body plan in land plants and charophycean algae, in this study, we compared their multicellular gametophyte and sporophyte generations based on their genes and genomes. We identified the developmental genes specifically expressed in different tissues/cells of C. braunii. Moreover, we explored the expression/function of these developmental genes in charalean algae in detail and discussed the evolution of the multicellular body plan in land plants and charophycean algae. We were also tried to introduce the exogenous gene into Chara cells using the native promoter of the Chara gene by microinjection and particle bombardment.


• Identification and analysis of a novel master transcription factor for sexual organ development in lant plants

  Yamaoka Shohei, KOHCHI Takayuki, NISHIHAMA Ryuichi, YAMATO Katsuyuki T., ISHIZAKI Kimitsune, SHIGENOBU Shuji, YAMAGUCHI Katsushi, YOSHITAKE Yoshihiro, INOUE Keisuke, ISHIDA Sakiko, SAITO Misaki, OKAHASHI Keitaro, BAO Haonan, NISHIDA Hiroyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kyoto University, 01 Apr. 2015 - 31 Mar. 2018

  Here we identified a master transcription factor MpBONOBO (MpBNB) that controls sexual organ development in the liverwort Marchantia polymorpha. MpBNB is expressed in the initial cells that are destined to develop into archegonia and antheridia, suggesting its major role in archegonial/antheridial development. Phylogenetic analysis suggested that MpBNB is a member of an evolutionarily conserved transcription factor family. Arabidopsis BNB1 and BNB2 are redundantly required for specification of generative cell in developing pollen, and are functionally replaceable with MpBNB. These findings suggest evolutionarily conserved role of BNB family proteins in the regulation of germ cell differentiation from gametophytes in land plants.


• Genome editing by microinjection in the model organism Marchantia polymorpha

  Yamato Katsuyuki, KOHCHI Takayuki, ISHIZAKI Kimitsune, TAKIKAWA Yoshihiro

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kindai University, 01 Apr. 2015 - 31 Mar. 2018

  DNA-free genome editing allows target-specific manipulation of a given genome. CRISPR RNA and Cas9 protein can be directly introduced into target cells and lead to genome-editing. We have successfully performed DNA-free genome editing mediated by microinjection of CRISPR/Cas9 complex in the liverwort Marchantia polymorpha. NOPPERABO1 (NOP1), of which loss-of-function mutation causes impaired air-chamber formation and thus can be found readily, was selected as a target gene. Custom gRNA and commercially available Cas9 protein were first allowed to form RNA-protein complex and then injected into single-cell sporelings by a laser thermal microinjector. One of the thalli grown from microinjected sporelings formed a sector that showed the nop1 mutant phenotype, and DNA isolated from the sector showed 5-bp deletion in the target coding sequence. This is the first demonstration of DNA-free genome-editing by microinjection in plants.


• Elucidation of physiological roles and regulation of the plasma membrane H+-ATPase

  Kinoshita Toshinori, KOHCHI Takayuki, ISHIZAKI Kimitsune, OHTA Hiroyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Nagoya University, 01 Apr. 2015 - 31 Mar. 2018

  The plasma membrane (PM) H+-ATPase in plants mediates numerous important physiological processes, such nutrient uptake in roots, stomatal opening, and phloem loading. However, the molecular and regulatory mechanisms of this enzyme is still remains unknown. In this study, we investigated regulatory mechanism and physiological roles of PM H+-ATPase in plants and found that PM H+-ATPase is regulated photosynthesis-dependent manner in Arabidopsis and Klebsormidium, and that PM H+-ATPase in rice roots are activated by one of nutrient silicon, which has important role for the stress tolerant in rice. Furthermore, we found that plant hormones brassinosteroid and auxin activate PM H+-ATPase in etiolated hypocotyl in Arabidopsis through their receptors, BRI1 and TIR1, respectively. These results provide important information for understanding molecular mechanism and physiological role of PM H+-ATPase in plant growth.


• Evolutionary and molecuar genetic studies to logics in plant development

  Kohchi Takayuki, ISHIZAKI Kimitsune, OSAKABE Keishi, SUETSUGU Noriyuki, SUGANO Shigeo, NAKAGAMI Hirofumi, NAKAMURA Yasukazu, NISHIHAMA Ryuichi, YAMAOKA Shohei

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Kyoto University, 28 Jun. 2013 - 31 Mar. 2018

  We studied the logics of plant development from a point of evolutionary view using the liverwort, Marchantia polymorpha, a member of basal land plant lineages as a model. We developed the genome database, genome editing vectors, and other molecular tools for experimental biology of M. polymorpha. By the comparative and reverse genetic studies of fundamental factors known to regulate angiosperm development, we showed that the common ancestor of land plants had acquired the basic system of plant development including auxin-dependent three-dimensional growth, light signaling pathways, cell division regulation, and environmental induction of sexual reproduction. Starting with the mutant analysis and its causal gene identification in M. polymorpha, we identified the master regulator, BONOBO, which determines the germline cell lineage in land plants. We concluded the evolutionary strategies to plant developmental biology are highly effective and informative.


• Development of research base to elucidate the mechanism of stem endodermis formation

  Hidehiro Fukaki, MIMURA TETSURO, ISHIZAKI KIMITSUNE, GOH TATSUAKI, TOYOKURA KOICHI

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Kobe University, 01 Apr. 2015 - 31 Mar. 2017

  On the cross section of the stem of the plant, tissues such as epidermis, cortex and endodermis are concentrically arranged from the outside. Radial patterns of such tissues are formed from undifferentiated tissues at the tip of the stem (shoot apical meristem), but their formation mechanisms are hardly elucidated. This research aims to understand the mechanism of radial pattern formation of the stem as a major objective, focusing on endodermis layer of the stem. The research infrastructure for the elucidation of the endodermis formation mechanism of stem was improved by trying to identify the endodermis stem cells in the shoot apical meristem and the genes associated with endodermis formation, through devising research materials and using observation techniques developed recently.


• 細胞壁再構成をともなう離生細胞間隙形成の分子機構

  石崎 公庸

  科学研究費補助金／新学術領域研究, Apr. 2015 - Mar. 2017, Principal investigator

  Competitive research funding


• Genomic studies of transcription factors and experimental analysis of their functions by genome editing in Marchantia polymorpha

  Kohchi Takayuki, NISHIHAMA Ryuichi, YAMAOKA Shohei, ISHIZAKI Kimitsune, SUGANO Shigeo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Kyoto University, 01 Apr. 2014 - 31 Mar. 2016

  Land plants acquired a system to coordinate developmental program with environment during evolution. Transcriptional regulation is one of the major mechanisms for the adaptive growth and development in plants. As a basal land plant, the liverwort Marchantia polymorpha is an attractive model organism with haploid-dominant life cycle. In addition, sophisticated experimental systems such as efficient genetic transformation and genome information have been developed in M. polymorpha. We deduced the entire set of genes for transcription factors from the draft genome sequences of M. polymorpha. To experimentally analyze gene function efficiently, the CRISPR/Cas9-mediated genome editing is a simple and straightforward method to disrupt genes of interest. We significantly improved the efficiency of genome editing by optimizing Cas9 expression and CRISPR design. Simple, rapid and efficient mutagenesis by CRISPR/Cas9 enabled systematic analysis of transcription factors in M. polymorpha.


• Analyses on photoregulation of the cell cycle and mechanisms of cell division in plants

  NISHIHAMA Ryuichi, KOHCHI Takayuki, ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kyoto University, 01 Apr. 2012 - 31 Mar. 2015

  In plants, light plays critical roles in processes that accompany with cell proliferation, such as organogenesis and regeneration. For the liverwort Marchantia polymorpha, a bryophyte, red light promotes cell proliferation. This research project revealed that red light regulates different steps of expression of a gene that regulates S-phase entry by signaling via photosynthesis and the red-light receptor phytochrome, and that the phytochrome also promotes isotropic cell growth. Functional analysis on the microtubule-based motor protein that is localized to the cell plate suggested that the protein may play a role in the transport of membrane vesicles for cell-plate formation. As this gene was found to be essential, a vector and a protocol for obtaining conditional knockout mutants were created.


• Search for sex determination genes in Marchantia polymorpha

  YAMATO Katsuyuki, KOHCH Takayuki, ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kinki University, 01 Apr. 2012 - 31 Mar. 2015

  Five sequences of a female genomic data were identified as X-linked by comparing with a male genomic data. RNA-seq data obtained from a variety of tissues were mapped onto the X-linked sequences, and genes expressed specifically in female sexual organs were searched. RT-PCR revealed one of the candidate genes was indeed expressed specifically in female sexual organs. The candidate X-linked gene is similar to those involved in regulation of DNA methylation.


• 細胞壁再構築をともなう離生細胞間隙形成の分子機構

  石崎 公庸

  科学研究費補助金／新学術領域研究, Apr. 2013 - Mar. 2015, Principal investigator

  Competitive research funding


• 環境変動下における生存戦略としての栄養繁殖機構の解析

  石崎 公庸

  科学研究費補助金／新学術領域研究, Apr. 2013 - Mar. 2015, Principal investigator

  Competitive research funding


• 植物の栄養繁殖におけるオーキシン機能メカニズムの解析

  石崎 公庸

  学術研究助成基金助成金／基盤研究(C), Apr. 2012 - Mar. 2015, Principal investigator

  Competitive research funding


• Systematic analysis of transcription factors in Marchantia polymorpha

  KOHCHI Takayuki, ISHIZAKI Kimitsune, NISHIHAMA Ryuichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Kyoto University, 01 Apr. 2012 - 31 Mar. 2014

  Transcription factors are major regulators for various biological processes including development and environmental responses. Arabidopsis thaliana and Physcomitrella patens have 2,000 and 1,200 transcription factors, respectively. The liverwort Marchantia polymorpha is an emerging model organism for developmental and evolutional studies. M. polymorpha occupies a crucial position in the evolution of land plant and has the haploid-dominant life cycle, which provides advantages over diploid plants for molecular genetic analysis. In this study, we identified ca. 300 transcription factors in M. polymorpha by searching our EST and RNA-sequence databases that include more than 20,000 genes. Surprisingly, M. polymorpha had 38 transcription factor families that are common numbers with A. thaliana. These results indicate that redundancy of transcription factors in M. polymorpha should be very low and that M. polymoroha provides suitable system for genetic analysis.


• Proteomic identification of dephosphorylated proteins by phosphatase protein

  FUKAO YOICHIRO, ISHIZAKI Kimitsune, FUJIWARA Masayuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Nara Institute of Science and Technology, 01 Apr. 2012 - 31 Mar. 2014

  Protein phosphorylation and dephosphorylation by protein kinases and phosphatases regulate many biological events in plants. To identify as many phosphoproteins as possible, several purification methods of phosphopeptides have been established. Here, we used two kind of phosphopeptides purification columns (PhosTio and PolyMac) and each method allowed identifying 771 and 641 phosphopeptides in rice cultured cells. Also, 913 and 802 phosphopeptides were identified in rice cultured cells treated with rice blast fungus. These results indicate that phosphorylation of several proteins are induced responded to pathogen attack and may have a role in disease resistances.


• Molecular genetic studies on flowering control by VOZ1 and VOZ2 in Arabidopsis

  KOHCHI Takayuki, ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kyoto University, 01 Apr. 2011 - 31 Mar. 2014

  The phase transition of vegetative to reproductive growth in plants is regulated by various environmental factors including day length and light quality. Two Arabidopsis thaliana genes, VASCULAR PLANT ONE ZINC FINGER1 and VOZ2 mediated phyB-dependent flowering regulation. By mutant complementation assays, VOZ1 and VOZ2 expressed in vascular bundles functioned as transcriptional regulators in nucleus. Genetic and molecular researches suggested that VOZ1 and VOZ2 promote growth-phase transition to flowering by down-regulating FLC and its closely related homologs MAF1 to MAF5 by a pathway that is independent to vernalization pathway.


• 環境変動下における生存戦略としての栄養生殖機構の解析

  ISHIZAKI Kimitsune

  Ministry of Education, Culture, Sports, Science and Technology-Japan, Grant-in-Aid for Scientific Research on Innovative Areas, Apr. 2011 - Mar. 2013, Principal investigator

  Competitive research funding


• Reverse genetics and recombination

  IIDA Shigeru, JOHZUKA Yasuyo, KINOSHITA Tetsu, ISHIZAKI Kimitsune, KOBAYASHI Hirokazu, NOGUCHI Hiroshi, WATANABE Kenji, JOHZUKA Yasuyo

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), University of Shizuoka, 2010 - 2012

  To facilitate reverse genetic approaches, which include homologous recombination-mediated gene targeting using positive-negative selection and gene tagging employing endogenous nDart1-related DNA transposons, we were attempting to elucidate recombination mechanisms and their regulation mechanisms associated with gene targeting and tagging. We also characterized the functions of the genes identified by gene targeting and tagging.


• フィトクロムによる植物成長相転換制御の基本プログラム

  ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Apr. 2010 - Mar. 2011, Principal investigator

  Competitive research funding


• Development of molecular tools for forward genetics in the liverwort Marchantia polymorpha

  KOHCHI Takayuki, ISHIZAKI Kimitsune, YAMATO Katsuyki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Kyoto University, 2010 - 2011

  The liverwort Marchantia polymorpha is an emerging model of evodevo studies in land plants. We developed molecular tools for forward genetics for M. polymorpha. DNA polymorphism between the standard accession Takaragaike-1 and the reference accession Kitashirakawa was systematically detected and used for genetic mapping with high resolution. We also applied the T-DNA tagging to isolate developmental mutants and to identify causal genes in M. polymorpha.


• Evolution of sex chromosomes in haploid genome

  YAMATO Katsuyuki, KOHCHI Takayuki, ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kyoto University, 2008 - 2010

  Among the genes identified in the Y chromosomal region under investigation, eight appears to have essential functions and thus are expected to have their homologs on the X chromosome. In fact, the Y-chromosomal M547D3.1 gene has its X-chromosomal partner M547D3.1F. Additional 39 X-chromosomal counterparts were obtained from female genomic data provided from JGI.


• 光情報による陸上植物生長相転換制御メカニズム

  ISHIZAKI Kimitsune

  Ministry of Education, Culture, Sports, Science and Technology-Japan, Grant-in-Aid for Young Scientists (B), Apr. 2008 - Mar. 2009, Principal investigator

  Competitive research funding


• 陸上植物における生殖成長相移行の基本プログラム

  ISHIZAKI Kimitsune

  Japan Society for the Promotion of Science, Grant-in-Aid for Research Activity start-up, Apr. 2007 - Mar. 2008, Principal investigator

  Competitive research funding