Research activity information

• Sep. 2017 一般社団法人産業応用工学会, Best Paper Award, Estimation of Calf Weight from Fixed-Point Stereo Camera Images Using Three-Dimensional Successive Cylindrical Model

  山下 歩, OHKAWA TAKENAO, 大山 憲二, 太田 能, 西出 亮, 本多 健

  International society


• Aug. 2016 Secretariat for AAAP2016, 17th AAAP Animal Science Congress Young Scientists Award, Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima

  Oyama H, Nishi K, Imamura K, Sakamoto S, Kawabe K, Okamoto S, HONDA TAKESHI, OYAMA KENJI, Shimogiri T

  International society

• Estimation of genetic parameters for carcass grading traits, image analysis traits, and monounsaturated fatty acids in Japanese Black cattle from Hyogo Prefecture.

  Namiko Kohama, Emi Yoshida, Tatsunori Masaki, Eiji Iwamoto, Moriyuki Fukushima, Takeshi Honda, Kenji Oyama

  Genetic parameters for carcass grading traits, image analysis traits, and monounsaturated fatty acid (MUFA) percentages were estimated in 29,942 Japanese Black cattle from Hyogo Prefecture. The analyzed traits included five carcass grading traits, two image analysis traits, fat area ratio and fineness index, and two MUFA traits, one measured in intermuscular fat using near-infrared spectroscopy (NIRS) and the other in intramuscular fat using gas chromatography (GC). The heritability estimates of image analysis traits and MUFA were moderate to high, ranging from 0.395 to 0.740, and it was considered that they could be improved simultaneously with carcass grading traits because no severe genetic antagonism was observed. Although the heritability of the NIRS-based intermuscular MUFA was slightly lower than that of the GC-based intramuscular MUFA, the genetic correlation between the two methods was as high as 0.804. These results indicate that the NIRS method can be used as an alternative evaluation procedure to predict MUFA in intramuscular fat in the longissimus muscle.

  Dec. 2021, Animal science journal = Nihon chikusan Gakkaiho, 92(1) (1), e13664, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of carcass traits, proportion of monounsaturated fatty acids and fineness index of marbling on the carcass unit price of Japanese Black cattle in Hyogo prefecture

  Namiko KOHAMA, Takayuki AKIYAMA, Moriyuki HUKUSHIMA, Takeshi HONDA, Kenji OYAMA

  Japanese Society of Animal Science, Nov. 2021, Nihon Chikusan Gakkaiho, 92(4) (4), 511 - 517

  Scientific journal


• A Novel Indicator for Evaluating the Change of Genetic Composition of a Developed Pig Strain

  Takeshi Honda, Kenji Oyama, Masahiro Sato

  Sep. 2021, The Japanese Journal of Swine Science, 58(3) (3), 125 - 133, Japanese

  [Refereed]


• Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima

  Hidemi OYAMA, Kiyoto IMAMURA, Shinichi SAKAMOTO, Kazutaka NISHI, Kotaro KAWABE, Shin OKAMOTO, Takeshi HONDA, Kenji OYAMA, Takeshi SHIMOGIRI

  Japanese Society of Animal Science, Aug. 2021, Nihon Chikusan Gakkaiho, 92(3) (3), 285 - 291

  Scientific journal


• Detection of candidate polymorphisms around the QTL for fat area ratio to rib eye area on BTA7 using whole‐genome resequencing in Japanese Black cattle

  Shinji Sasazaki, Fuki Kawaguchi, Ayaka Nakajima, Raito Yamamoto, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen

  Wiley, Jan. 2020, Animal Science Journal, 91(1) (1), e13335

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimation of genetic parameters of defective appearances in Japanese Black heifer calves in Kagoshima

  Oyama H, Imamura K, Sakamoto S, Nishi K, Kawabe K, Okamoto S, Honda T, Oyama K, Shimogiri T

  Jan. 2020, Animal Science Journal, 91, e13338

  [Refereed]

  Scientific journal


• Estimation of lethal equivalents in the Tajima beef cattle

  Takeshi Honda, Kenji Oyama

  Lead, Nov. 2019, 日本畜産学会報, 90(4) (4), 337 - 340, Japanese

  [Refereed]

  Scientific journal


• Pool-based genome-wide association study identified novel candidate regions on BTA9 and 14 for oleic acid percentage in Japanese Black cattle

  Fuki Kawaguchi, Hiroto Kigoshi, Ayaka Nakajima, Yuta Matsumoto, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Wiley, Aug. 2018, Animal Science Journal, 89(8) (8), 1060 - 1066, English

  [Refereed]

  Scientific journal


• A genome-wide association study for fat-related traits computed by image analysis in Japanese Black cattle

  Ayaka Nakajima, Fuki Kawaguchi, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Wiley, May 2018, Animal Science Journal, 89(5) (5), 743 - 751, English

  [Refereed]

  Scientific journal


• Calf Weight Estimation with Stereo Camera Using Three-Dimensional Successive Cylindrical Model

  Ayumi Yamashita, Takenao Ohkawa, Kenji Oyama, Chikara Ohta, Ryo Nishide, Takeshi Honda

  Various studies have been conducted on methods for estimating the weight of cattle. In this study, we propose a method to estimate body weight by modeling the shape of calf using three-dimensional information extracted from the stereo images. Initially, a stereo camera is set with two fixed network cameras, to take a motion image of a calf. Three-dimensional coordinate is calcu

  The Institute of Industrial Applications Engineers, Jan. 2018, Journal of the Institute of Industrial Applications Engineers (JIIAE), 6(1) (1), 39 - 46, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 鹿児島県産黒毛和種における損徴発生状況に関する調査研究

  小山 秀美, 今村 清人, 坂元 信一, 西 和隆, 井上 慶一, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  Defects in appearance of Japanese Black cattle are not only impairing the characteristics of the breed but also inflicting economic losses on farmers in Japan. The objective of this study was to investigate the incidence of the four kinds of defects using 511,337 calf test records of Japanese Black heifer calves born in Kagoshima prefecture in April 1999 through March 2015. The defects included were white spotting (WS), tongue's defect (TD), nipple's defect (ND) and defect of coat color (DC). The defect incidence rate ranged from 0.29% for DC to 3.68% for WS. All incidences increased from 1999 to 2015. In addition, site of occurrence for WS has changed, and a decreased trend in ratio of udder-specific WS was observed. Regression coefficients of the incidences of WS on the level of inbreeding were significantly positive (P<0.05). The defect incidence of daughters was significantly different between normal and defective dams (P<0.01) except for DC. Moreover, highly positive correlations were found between the sire-by-sire incidence rates crossed with normal dams and defective dams in WS and TD, suggesting that sires could be influential factor for the incidence. Our results showed the possibility to reduce the appearance defects of Japanese Black by considering the mates.

  Japanese Society of Animal Science, Nov. 2017, 日本畜産学会報, 88(4) (4), 425 - 430, Japanese

  [Refereed]

  Scientific journal


• Preservation of Genetic Diversity of the Asian Native Goats

  Honda T, Shibano M, Matsumoto H, Sasazaki S, Oyama K, Mannen H

  Sep. 2017, Animal Veterinary Sciences, 5, 69 - 72, English

  [Refereed]

  Scientific journal


• Estimation of Calf Weight from Fixed-Point Stereo Camera Images Using Three-Dimensional Successive Cylindrical Model

  Ayumi Yamashita, Takenao Ohkawa, Kenji Oyama, Chikara Ohta, Ryo Nishide, Takeshi Honda

  Various studies have been conducted on methods for estimating the weight of cattle. In this study, we propose a method to estimate body weight by modeling the shape of calf using three-dimensional information extracted from the stereo images. Initially, a stereo camera is set with two fixed network cameras, to take a motion image of a calf. Three-dimensional coordinate is calcu

  The Institute of Industrial Applications Engineers, Jul. 2017, The 5th IIAE International Conference on Intelligent Systems and Image Processing 2017 (ICISIP2017), 247 - 254, English

  [Refereed]

  International conference proceedings


• Genetic relationships between meat quality traits and fatty acid composition in Japanese black cattle

  Keiichi Inoue, Noriaki Shoji, Takeshi Honda, Kenji Oyama

  Genetic parameters were estimated to investigate the relationships between meat quality traits and fatty acid composition from 11855 Japanese Black cattle. The meat quality traits included beef marbling score (BMS), beef color score, firmness of beef (FIR), texture of beef (TEX) and beef fat color score (BFS). The data on fatty acid composition included oleic acid and monounsaturated fatty acids (MUFA) contents, the ratio of MUFA to saturated fatty acids (MUS) and the ratio of elongation. The heritability estimates for meat quality traits ranged from moderate (0.30) to high (0.72). The strong genetic correlations between them were useful for simultaneous genetic improvement. In addition, high heritability estimates of fatty acid composition, ranging from 0.60 to 0.63, indicated that they could also be improved genetically. The genetic correlations of fatty acid composition with BMS, FIR and TEX were weak and negative. In contrast, positive and stronger genetic correlations were found between BFS and fatty acid composition, in particular, related to the level of unsaturation (0.77 and 0.79 for MUFA and MUS, respectively). This implies that improving the level of unsaturation makes fat darker (more yellow) and thus requires balancing with BFS.

  WILEY-BLACKWELL, Jan. 2017, ANIMAL SCIENCE JOURNAL, 88(1) (1), 11 - 18, English

  [Refereed]

  Scientific journal


• Inferring phenotypic causal structures among meat quality traits and the application of a structural equation model in Japanese Black cattle

  K. Inoue, B. D. Valente, N. Shoji, T. Honda, K. Oyama, G. J. M. Rosa

  Meat quality is one of the most important traits determining carcass price in the Japanese beef market. Optimized breeding goals and management practices for the improvement of meat quality traits requires knowledge regarding any potential functional relationships between them. In this context, the objective of this research was to infer phenotypic causal networks involving beef marbling score (BMS), beef color score (BCL), firmness of beef (FIR), texture of beef (TEX), beef fat color score (BFS), and the ratio of MUFA to SFA (MUS) from 11,855 Japanese Black cattle. The inductive causation (IC) algorithm was implemented to search for causal links among these traits and was conditionally applied to their joint distribution on genetic effects. This information was obtained from the posterior distribution of the residual (co)variance matrix of a standard Bayesian multiple trait model (MTM). Apart from BFS, the IC algorithm implemented with 95% highest posterior density (HPD) intervals detected only undirected links among the traits. However, as a result of the application of 80% HPD intervals, more links were recovered and the undirected links were changed into directed ones, except between FIR and TEX. Therefore, 2 competing causal networks resulting from the IC algorithm, with either the arrow FIR -> TEX or the arrow FIR -> TEX, were fitted using a structural equation model () to infer causal structure coefficients between the selected traits. Results indicated similar genetic and residual variances as well as genetic correlation estimates from both structural equation models. The genetic variances in BMS, FIR, and TEX from the structural equation models were smaller than those obtained from the MTM. In contrast, the variances in BCL, BFS, and MUS, which were not conditioned on any of the other traits in the causal structures, had no significant differences between the structural equation model and MTM. The structural coefficient for the path from MUS (BCL) to BMS showed that a 1-unit improvement in MUS (BCL) resulted in an increase of 0.85 or 1.45 (an decrease of 0.52 or 0.54) in BMS in the causal structures. The analysis revealed some interesting functional relationships, direct genetic effects, and the magnitude of the causal effects between these traits, for example, indicating that BMS would be affected by interventions on MUS and BCL. In addition, if interventions existed in this scenario, a breeding strategy based only on the MTM would lead to a mistaken selection for BMS.

  AMER SOC ANIMAL SCIENCE, Oct. 2016, JOURNAL OF ANIMAL SCIENCE, 94(10) (10), 4133 - 4142, English

  [Refereed]

  Scientific journal


• Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.

  H. Ozaki, T. Tamura, K. Fukazawa, Y. Uemoto, M. Nishio, E. Kobayashi, T. Matsuhashi, S. Maruyama, T. Honda, K. Oyama, S. Sasazaki, H. Mannen

  AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, English


• Identification of polymorphisms associated with oleic acid percentage by pool-based genome-wide association study in Japanese Black cattle.

  F. Kawaguchi, A. Nakajima, Y. Matsumoto, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, T. Honda, K. Oyama, H. Mannen, S. Sasazaki

  AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, English


• Genome-wide association study identifies a QTL for fat percentage in ribeye area on BTA10 in Japanese Black cattle.

  A. Inoue, T. Nakajima, A. Nakajima, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, K. Oyama, T. Honda, H. Mannen, S. Sasazaki

  AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, English


• Estimation of genetic parameters for carcass defects of Japanese Black cattle in Kagoshima

  Kazutaka Nishi, Takeshi Shimogiri, Akinori Kusano, Shinichi Sakamoto, Kiyomi Shiromoto, Kotaro Kawabe, Shin Okamoto, Takeshi Honda, Kenji Oyama

  Cattle exhibit a range of carcass defects, including blood splash (BLS), intramuscular edema (INE), muscle steatosis (MUS), bruising (BR), trim loss (TRL) and others (OTH). These defects lower the carcass value and can result in significant economic loss to producers. We estimated the incidence, relationship with inbreeding coefficients and genetic parameters of carcass defects in Japanese Black cattle using 561 619 carcass records from Kagoshima, Japan during April 1988 through March 2011. The defect incidence ranged from 0.22% for TRL to 5.73% for BR. The incidence of MUS and BR increased from 1.21% to 6.57% and from 1.06% to 9.31%, respectively. The incidence of INE peaked at 7.44% in 1999 and decreased thereafter. We observed a positive linear relationship between the defect incidence and the inbreeding coefficients in MUS, BR and TRL (P<0.01). The heritabilities estimated by univariate animal model with Gibbs sampling for BLS, INE, MUS, BR and TRL were 0.24, 0.06, 0.18, 0.05 and 0.02, respectively. The contribution of farm variance to phenotypic variance was negligible (0.01 to 0.04). Significant genetic correlations of TRL were estimated with MUS (0.63) and BR (0.63). Our results suggest that genetic factors contribute to the incidence of BLS and MUS.

  WILEY-BLACKWELL, May 2016, ANIMAL SCIENCE JOURNAL, 87(5) (5), 655 - 660, English

  [Refereed]

  Scientific journal


• Economic losses related to internal diseases in Japanese black cattle

  Keiichi Inoue, Takeshi Honda, Kenji Oyama

  The objective of this study was to estimate the direct economic losses due to the condemnation of the liver and large intestine because of internal diseases (multifocal necrosis in the liver (MNL) and inflammation of the large intestine (ILI)), and the indirect losses because of reductions in carcass performance from MNL, bovine abdominal fat necrosis (BFN) and ILI using data from 5383 Japanese Black cattle. Direct losses were estimated by multiplying the price of the condemned part by the frequency of its occurrence owing to the disease. Similarly, indirect losses were estimated as the product of unit carcass price and reduction in carcass weight (CW) due to the disease. The direct impact on the beef cattle industry from MNL and ILI was estimated at around $1.29 million (US$1 = (sic)120) per year. A least-squares analysis showed that MNL had no influence on any carcass trait, whereas BFN and ILI significantly reduced CW, rib eye area and darkened the beef. ILI also reduced rib thickness. The indirect losses from BFN and ILI were estimated as a maximum of $131.7 and $256.4 per animal and around $6.26 million and $4.03 million for the industry, respectively, mostly because of the reduction in CW.

  WILEY-BLACKWELL, May 2016, ANIMAL SCIENCE JOURNAL, 87(5) (5), 736 - 741, English

  [Refereed]

  Scientific journal


• Change of fatty acid composition of the lumbar longissimus during the final stage of fattening in the Japanese Black cattle

  Takeshi Honda, Takafumi Ishida, Ikuo Kobayashi, Yusuke Oguri, Yuto Mizuno, Hideyuki Mannen, Hiroaki Iwaisaki, Shiro Kuge, Kunihiko Saito, Kenji Oyama

  Consideration of the shortened fattening period seems to be worthwhile for the realization of profitable beef production. In this study, change of fatty acid composition of the lumbar longissimus during the final stage of fattening was investigated in Japanese Black cattle. Each of 110 fattening animals was sampled three times: the initial two samples were taken by biopsy (25.7 months and 27.5 months on average) and the final one was from carcasses (29.9 months on average). Preliminary analysis indicated that removing muscle tissues from the constant body position of the living animals should be essential for sampling. Average monounsaturated fatty acids (MUFA) at three sampling points were 58.1%, 58.5% and 60.5%, and the differences of the third sampling with the first and second samplings were significant. Both in steers and heifers, MUFA also increased as the fattening stage proceeded, and MUFA of the heifers at all the sampling points were significantly higher than those of the steers. The increasing rate of MUFA rose from 0.21 percentage points (pp)/month at period 1 (from the first sampling to the second sampling) to 0.84 pp/month at period 2 (from the second sampling to the slaughter).

  WILEY-BLACKWELL, Apr. 2016, ANIMAL SCIENCE JOURNAL, 87(4) (4), 578 - 583, English

  [Refereed]

  Scientific journal


• Extraction of Interaction Information Between Breeding Cows with GPS and Its Application to Estrus Detection

  Kengo Matsumoto, Kenji Oyama, Takeshi Honda, Takenao Ohkawa

  This study focuses on bovine sociality and proposes a new method of detecting estrus through the detection and the analysis of interactions between cows. In the proposed method, we mainly trace bovine behavior when an animal approaches other cows based on their time-series location data measured by a GPS installed on each animal. The cow's estrus is detected by analyzing the interaction information extracted from the behavior. Our proposed method experimentally obtained more accurate results than the existing method of estrus detection.

  IEEE, 2016, 2016 INTERNATIONAL JOINT CONFERENCE ON NEURAL NETWORKS (IJCNN), 2967 - 2972, English

  [Refereed]

  International conference proceedings


• A Method of Detecting Interaction Between Breeding Cows from Time-series Bird's Eye Pictures of Pasture

  Yoji Hosomi, Kenji Oyama, Takeshi Honda, Takenao Ohkawa

  Breeding cows are known to engage in sociality, in which they interact and form groups. This paper proposes a method of detecting the interaction between breeding cows from time-series pictures of pastures by a similar image retrieval method using a Bag of Visual Words. We divided the interaction detection into three tasks: detecting a pair of cows in an interaction, pinpointing the time and the place that the interaction was observed and identifying its type. This paper focuses on the identifying task. In the proposed method, a similar image retrieval method is used to classify the interaction, and the information from the states of preceding and succeeding the interaction is considered to improve the accuracy of the interaction classification. The effectiveness of the proposed method was confirmed experimentally.

  IEEE, 2016, 2016 INTERNATIONAL JOINT CONFERENCE ON NEURAL NETWORKS (IJCNN), 2973 - 2978, English

  [Refereed]

  International conference proceedings


• 放牧場の俯瞰動画データを用いた繁殖牛のインタラクション検出

  HOSOMI YOJI, OHKAWA TAKENAO, OYAMA Kenji, HONDA Takeshi

  May 2015, 電気学会 第62回情報システム研究会, IS-15-009, Japanese

  Symposium


• GPSを利用した放牧牛のインタラクション情報の取得とその分析

  MATSUMOTO Kengo, OHKAWA TAKENAO, OYAMA Kenji, HONDA Takeshi

  May 2015, 電気学会 第62回情報システム研究会, IS-15-001, Japanese

  Symposium


• Genetic relationships between internal diseases diagnosed at slaughter and carcass traits in Japanese Black cattle

  Inoue K, Honda T, Oyama K

  May 2015, Journal of Animal Science, 93(6) (6), 2714 - 2721, English

  [Refereed]

  Scientific journal


• Searching for phenotypic causal links among meat quality traits in Japanese Black cattle

  Inoue K, Valente DB, Shoji N, HONDA TAKESHI, OYAMA KENJI, Rosa JMG

  Aug. 2014, Proceedings of 10th World Congress on Genetics Applied to Livestock Production, 749, English

  [Refereed]

  International conference proceedings


• 兵庫系黒毛和種における育種集団毎の遺伝的多様性評価

  福島 護之, 本多 健, 小路 怜子, 小浜 菜美子, 秋山 敬孝, 坂瀬 充洋, 岡 章生, 大山 憲二

  (公社)日本畜産学会, Sep. 2013, 日本畜産学会大会講演要旨集, 117回, 65 - 65, Japanese


• Comparative analysis on gene expression profiles in longissimus dorsi muscle of Japanese Black cattle

  MATSUMOTO H, OGURI Y, MIZUNO Y, ISHIDA T, KOBAYASHI I, IWAISAKI H, SAITO K, SASAGO N, KOBAYASHI E, KUGE S, HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

  Recently, fatty acid composition has been recognized as an important economic trait of Japanese Black cattle. The aim of this study was to seek for candidate genes which might be useful for beef breeding to increase the mono-unsaturated fatty acid (MUFA) level. For this purpose, we compared gene expression profile of Japanese Black cattle which showed high percentage of MUFA in

  日本動物遺伝育種学会, Jun. 2013, The Journal of Animal Genetics, 41(1) (1), 7 - 14, Japanese

  [Refereed]

  Scientific journal


• Sampling method for estimating neutral allele frequency in a pedigreed population

  Takeshi Honda, Shinji Sasazaki, Kenji Oyama, Fumio Mukai, Tetsuro Nomura

  The estimated allele frequency at neutral DNA marker loci is a fundamental parameter for establishing a conservation scheme for a set of livestock breeds. In this study, we propose a novel minimum distance (MD) method for estimating neutral allele frequencies, which minimizes the error by the use of pedigree information. Using computer simulation and actual microsatellite data for a pedigreed cattle population, the performance of the proposed method was compared with that of conventional random sampling (RND). MD manifests a promising superiority to RND, irrespective of the sample size and the depth of pedigree. It is theoretically demonstrated that the error of the estimates depends on both the sample size and the distance of founder allele frequencies between the sampled and the non-sampled individuals. MD samples individuals so as to minimize the latter source of estimation error.

  WILEY-BLACKWELL, Jun. 2012, JOURNAL OF ANIMAL BREEDING AND GENETICS, 129(3) (3), 226 - 233, English

  [Refereed]

  Scientific journal


• Heritabilities and genetic correlations of fatty acid compositions in longissimus muscle lipid with carcass traits in Japanese Black cattle

  T. Nogi, T. Honda, F. Mukai, T. Okagaki, K. Oyama

  Fatty acid composition and carcass traits of 2,275 Japanese Black steers and heifers were analyzed to estimate the heritabilities and genetic correlations using the REML procedure. Slices of LM at the 6th to 7th rib section were minced and homogenized, and total lipids were extracted for the analysis by a gas chromatograph. Oleic acid accounted for the majority (51.3%), followed by palmitic (26.4%) and stearic (10.8%) acids. Heritabilities of carcass traits were moderate to high, ranging from 0.34 to 0.61, and heritabilities of individual fatty acids varied largely from 0.00 to 0.78. Those of MUFA, SFA, and PUFA were estimated to be 0.68, 0.66, and 0.47, respectively. Predicted breeding values for MUFA in 99 sires ranged from -3.0 to 5.4%. Genetic correlations of fatty acid compositions with carcass traits were generally weak (-0.28 to 0.39). Low but positive genetic correlations were obtained between beef marbling, on which emphasis of selection has been placed, and oleic acid (0.19) or MUFA (0.23). The results indicated the possibility not only for genetic improvement in fat quality traits but also simultaneous improvements with carcass traits by appropriate selection program.

  AMER SOC ANIMAL SCIENCE, Mar. 2011, JOURNAL OF ANIMAL SCIENCE, 89(3) (3), 615 - 621, English

  [Refereed]

  Scientific journal


• Estimates of milk yield until 90 days after calving in Japanese Black cows

  TSUJI Emi, HONDA Takeshi, KUGE Shiro, OYAMA Kenji

  2011, 肉用牛研究会報, 90, 10 - 14, Japanese

  [Refereed]

  Scientific journal


• Demographic analysis of breeding structure in Japanese thoroughbred population

  Jun Yamashita, Hironori Oki, Telhisa Hasegawa, Takeshi Honda, Tetsuro Nomura

  To investigate the breeding structure in the Japanese Thoroughbred population, we applied a demographic analysis to the populations of foals produced from 1978 to 2005. The migration rate estimated from the proportion of foals produced by imported breeding horses was around 40% over the investigated period. After early 1990s, the migration rate through stallions imported from USA sharply increased. The average generation interval was within range of 10.5-11.5 years. The longer generation interval of Thoroughbred was considered to be a reflection of the fact that Thoroughbred horses begin breeding only after completing their performance in races. After the peak of 729 in 1993, the number of sires of foals progressively declined to 358 in 2005. Although the coefficient of variation of the progeny number of sires was within range of 1.0-1.2 until early 1990s, it gradually increased and reached the value of 1.6-1.7 in recent years. The effective number of sires consistently decreased after the peak of 302.6 in 1992, and reached 120-130 in recent years, which is 25-30% of the actual number of sires. In parallel, the demographic estimate of the effective population size declined after early 1990s. The main cause of the observed change in the breeding structure was inferred to be the intensive use of a limited number of stallions for breeding.

  Jun. 2010, Journal of Equine Science, 21(2) (2), 11 - 16, English

  [Refereed]

  Scientific journal


• Genetic variability of semen characteristics in Japanese Black bulls

  NISHIMURA Kenichi, HONDA Takeshi, OYAMA Kenji

  2010, The Journal of Animal Genetics, 38(2) (2), 73 - 76, English

  [Refereed]

  Scientific journal


• Gene dropping analysis of ancestral contributions and allele survival in Japanese Thoroughbred population

  Jun Yamashita, Hironori Oki, Telhisa Hasegawa, Takeshi Honda, Tetsuro Nomura

  Genetic contributions of nine historically important ancestors and allelic diversity in the Japanese Thoroughbred population were examined by applying the gene dropping simulation to the foals produced from 1978 to 2005. Full pedigree records traced to ancestors (base animals) born around 1890 were used for the simulation. Alleles originated from some of the historically important ancestors were found to be at risk of future extinction, although their genetic contributions to the foal population have increased during the last three decades. The proportion of surviving alleles to the total alleles assigned to the base animals was 8.0% in the foal population in 2005, suggesting that a large part of genetic variability contained in the base animals is extinct in the current population.

  2010, Journal of Equine Science, 21(3) (3), 39 - 45, English

  [Refereed]

  Scientific journal


• Simulation Study on Parentage Analysis with SNPs in the Japanese Black Cattle Population

  Takeshi Honda, Tomohiro Katsuta, Fumio Mukai

  Parentage tests using polymorphic DNA marker are commonly performed to avoid incorrect recording of the parental information of livestock animals, and single-nucleotide polymorphisms (SNPs) are becoming the method of choice. In Japanese Black cattle, parentage tests based on the exclusion method using microsatellite markers are currently conducted; however, an alternative SNP system aimed at parentage tests has recently been developed. In the present study, two types of simulations were conducted using the pedigree data of two subpopulations in the breed (subpopulations of Hyogo and Shimane prefectures) in order to examine the effect of actual genetic and breeding structures. The first simulation (simulation 1) investigated the usefulness of SNPs for excluding a close relative of the true sire; the second one (simulation 2) investigated the accuracy of sire identification tests for multiple full-sib putative sires by a combined method of exclusion and paternity assignment based on the LOD score. The success rates of excluding a single full-sib and sire of the true sires were, respectively, 0.9915 and 0.9852 in Hyogo and 0.9848 and 0.9852 in Shimane, when 50 SNPs with minor allele frequency (MAF: q) of 0.25 <= q <= 0.35 were used in simulation 1. The success rates of sire identification tests based solely on the exclusion method were relatively low in simulation 2. However, assuming that 50 SNPs with MAF of 0.25 <= q <= 0.35 or 0.45 <= q <= 0.5 were available, the total success rates including achievements due to paternity assignment were, respectively, 0.9430 and 0.9681 in Hyogo and 0.8999 and 0.9399 for Shimane, even when each true sire was assumed to compete with 50 full-sibs.

  ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Oct. 2009, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 22(10) (10), 1351 - 1358, English

  [Refereed]

  Scientific journal


• Number of Calves Produced at Specified Age as a Measure of Reproductive Performance in Beef Cattle under Artificially-Inseminated Breeding Scheme

  Kenji Oyama, Shinya Fujiwara, Tomohiro Katsuta, Takeshi Honda, Fumio Mukai

  Reproductive abilities in beef cattle herds are receiving increased attention due to recent rises in production costs. To achieve more efficient management, a measure of fertility, namely the number of calves produced at k yr of age (NCPk), was developed and its genetic parameters were estimated from Japanese Black cows by restricted maximum likelihood procedures. The k examined were distributed from 2 to 10 yr of age and NCP(2) averaged 1.077 calves over 43,536 cows. The averages increased by approximately 0.9 calf with each additional 1 yr increment in k. Heritabilities of NCP(k) were estimated to be low ranging from 0.083 to 0.162, which seemingly suggested a difficulty of genetic improvement. However, large genetic variation and high accuracy were observed in predicted breeding values of NCP(k). For example, the breeding values of NCP(7) were predicted between -0.303 and +0.213 with average accuracy of 0.607 for cows with observations. Genetic correlations among different k were generally high and positive (0.474 to 0.995). The analyses showed that at least NCP(4) was required to maintain the genetic correlations of 0.8 or higher with subsequent NCP(k). Also NCP(5) maintained the genetic correlations of 0.9 or higher with subsequent NCP(k). The results suggested some possibilities for NCP(k) to be a selection criterion considering its genetic variation, high accuracy and consistency with subsequent performance.

  ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Feb. 2009, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 22(2) (2), 162 - 167, English

  [Refereed]

  Scientific journal


• Evaluation of genetic diversity in Japanese Brown cattle population by pedigree analysis

  T Honda, T Fujii, T Nomura, F Mukai

  The Japanese Brown is the second most common domestic beef breed in Japan. However, nowadays this breed is facing reduction in numbers because of pressure from a profitable domestic breed. This breed is uniformly characterized by its brown coat colour, but is comprised of two isolated sub-breeds, Kumamoto and Kouchi, each possessing a different gene pool. Pedigree analyses were carried out for the two sub-breeds using the pedigree records of animals born from 1970 to 2000. The effective population size has been found to be consistently reducing during the last three decades in both sub-breeds. The current effective sizes were estimated to be 25.5 and 6.0 for the Kumamoto and Kouchi sub-breeds, respectively. The estimate of the effective number of founders (N-ef) in the Kumamoto sub-breed decreased from 152.1 to 74.4; that of non-founders (N-enf), from 41.7 to 5.3; and that of founder genome equivalents (N-ge), from 32.7 to 4.9. The corresponding changes in the Kouchi sub-breed were from 108.2 to 79.4, 16.2 to 4.1, and 14.1 to 3.9. Increasing differences between the two genetic diversity indices in the sub-breeds indicate that the greater part of the reduction of genetic diversity can be attributed to genetic drift that accumulated in the non-founder generations. A comparison with published estimates for several cattle breeds suggests the extremely limited genetic diversity of Japanese Brown. In addition to the avoidance of further reduction of genetic diversity, it will be important to counteract the process of breed decline by establishing a production system to efficiently utilize the unique characteristics of this breed and by developing links between the breed and products with market value.

  BLACKWELL PUBLISHING, Jun. 2006, JOURNAL OF ANIMAL BREEDING AND GENETICS, 123(3) (3), 172 - 179, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Monitoring and preservation of genetic diversity in livestock breeds: a case study of the Japanese Black cattle population

  Nomura Tetsuro, MUKA Fumio, Honda Takeshi

  2006, Current Topics in Genetics, Vol.1 pp59-71, English

  [Refereed]


• Prediction of inbreeding in commercial females maintained by rotational mating with partially isolated sire lines

  T Honda, T Nomura, F Mukai

  The solution to the inbreeding problem for livestock breeds in commercial use is often complicated by hierarchical population structure, in which favourable genes are accumulated in the upper level of the hierarchy (breeding population) by artificial selection and the genetic progress achieved is transferred to the lower level through migration of males. When the breeding population is subdivided into several isolated lines, rotational mating with the lines has been shown to be quite an effective system to reduce the short- and long-term inbreeding of commercial females in the lower level. In practice, however, some amount of migration should be allowed among the lines to reduce the rate of inbreeding in each line. In this study, we developed the recurrence equation for the inbreeding coefficient of the commercial females maintained by the rotational mating with partially isolated lines. Numerical computations were carried out to evaluate the effect of the migration on the efficiency of the rotational mating. It was shown that even with a small amount of migration among the lines, the inbreeding of commercial females is substantially inflated. However, when four or five lines are available, the inbreeding coefficient of commercial females can be suppressed to an acceptable level, irrespective of the effective size of line and the migration rate. Application of the mating system to the population of Japanese Black cattle was also examined.

  BLACKWELL PUBLISHING, Oct. 2005, JOURNAL OF ANIMAL BREEDING AND GENETICS, 122(5) (5), 340 - 348, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Conservation of genetic diversity in the Japanese Black cattle population by the construction of partially isolated lines

  T Honda, T Nomura, F Mukai

  (I)n the Japanese Black cattle population, five genetically divergent subpopulations have played important roles as suppliers of breeding stocks to the entire breed. We supposed a situation where five lines were constructed from each of the five subpopulations, and applied to this set of lines a management plan to conserve genetic diversity. Assuming that the male migration pattern among the lines followed the island model, we assessed the optimum male migration rate and required male numbers in each line, satisfying the following three conditions simultaneously: (i) the rate of inbreeding in each line was below 0.01 per generation; (ii) at least 97% of the initial genetic diversity was preserved after 10 generations; and (iii) more than 50% of the genes in an initial line were retained in the line after 10 generations. We found that approximately one breeding male should be selected per year and one breeding male should be exchanged among the lines per generation in each line to satisfy these three conditions. Numerical analysis with the migration rates actually observed among the five subpopulations demonstrated that the initial genetic differentiation among the lines was rapidly decayed by an asymmetrical migration pattern. For a successful plan, migration among lines should be strictly managed.

  BLACKWELL VERLAG GMBH, Jun. 2005, JOURNAL OF ANIMAL BREEDING AND GENETICS, 122(3) (3), 188 - 194, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 但馬牛集団のジーンドロッピング法による系統分類の試み

  福島 護之, 坂瀬 充洋, 野田 昌信, 武田 和士, 上野 悟, 本多 健, 大山 憲二, 向井 文雄

  但馬牛における始祖個体遺伝子の消長をジーンドロッピング法で調べ，その結果を基に普遍的な系統分類の方法を検討した．1 現存牛の血統を可能な限り遡り出現した牛のうち名号，生年月日が明らかで，産地が兵庫県内の牛を始祖個体としたところ，条件を全て満たす個体は1,461頭(累計遺伝的寄与率0.773)となった．系統分類のための始祖個体は，Pr(lost)の小さい100頭に限定した．2 始祖個体の生産地は城崎郡14頭(累積寄与率0.0314)，出石郡2頭(累積寄与率0.0002)，美方郡82頭(累積寄与率0.637)と養父郡2頭であったが，当時の郡境からは養父郡の2頭は美方郡に分類されていると考えられ，美方郡産が84頭にのぼり寄与率が高かった．3 現存個体27,138頭に対する始祖個体からの遺伝的寄与率を計算し，その結果を基に主成分分析を行った．第3主成分までで約85％を説明でき，現在個体の頭数割合が3.7～25.9％の8グループに分類できた．4 各グループ内及びグループ間の平均血縁係数から，G1～G4とG8ではグループ間の平均血縁係数がグループ内の平均血縁係数よりも高いグループが存在し，近年は系統外の種雄牛との交配が増加したことに起因すると考えられた．G5～G7では，グループ内の平均血縁係数が最も高く，依然，系統内交配が主流であると考えられた．5 グループ毎の近交係数は，16,143頭の結果からG1～G4では13.0～17.8％と低く，G5～G8で18.7～23.2％と高い傾向にあった．6 グループ毎の枝肉重量の育種価の平均は-8.1～-2.7とグループ間でばらついていた．BMS育種価の平均に関しても，0.63～1.00とばらついていたが，各グループともに最大値をみると1.477～2.137と能力が高い個体から含まれていた．以上の結果から，ジーンドロッピング法により選定した100頭の始祖個体を元にした系統分類法によって現在の但馬牛を分類することが可能であり，頭数や産肉能力から見ても遺伝的多様性を保持しつつ，今後の改良が可能であることが明らかとなった．

  兵庫県立農林水産技術総合センター, 2005, 兵庫県立農林水産技術総合センター研究報告〔畜産編〕, 41 16-21(41) (41), 16 - 21, Japanese

  [Refereed]

  Scientific journal


• Genealogical relationship between pedigree and microsatellite information and analysis of genetic structure of a highly inbred Japanese black cattle strain

  S Sasazaki, T Honda, M Fukushima, K Oyama, H Mannen, F Mukai, S Tsuji

  Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated-low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.

  ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Oct. 2004, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(10) (10), 1355 - 1359, English

  Scientific journal


• Reduction of inbreeding in commercial females by rotational mating with several sire lines

  T Honda, T Nomura, F Mukai

  A mating system to reduce the inbreeding of commercial females in the lower level was examined theoretically, assuming a hierarchical breed structure, in which favorable genes are accumulated in the upper level by artificial selection and the. achieved genetic progress is transferred to the lower level through migration of males. The mating system examined was rotational mating with several closed sire lines in the upper level. Using the group coancestry theory, we derived recurrence equations for the inbreeding coefficient of the commercial females. The asymptotic inbreeding coefficient was also derived. Numerical computations showed that the critical factor for determining the inbreeding is the number of sire lines, and that the size of each sire line has a marginal effect. If four or five sire lines were available, rotational mating was found to be quite an effective system to reduce the short- and long-term inbreeding of the commercial females, irrespective of the effective size of each sire line. Oscillation of the inbreeding coefficient under rotational mating with initially related sire lines could be minimized by avoiding the consecutive use of highly related lines. Extensions and perspectives of the system are discussed in relation to practical application.

  Lead, E D P SCIENCES, Sep. 2004, GENETICS SELECTION EVOLUTION, 36(5) (5), 509 - 526, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Monitoring of genetic diversity in the Japanese Black cattle population by the use of pedigree information

  T Honda, T Nomura, Y Yamaguchi, F Mukai

  The gene pool of the Japanese Black cattle has been completely closed to foreign breeds during the last 100 years. Genetic diversity of the Japanese Black cattle from 1960 to 2000 was monitored with three estimates of effective number of ancestors. Founder genome equivalent (N-ge) accounts for all the causes of reduction of diversity. Effective number of founders (N-ef) and non-founders (N-enf) explain reduced diversity because of unequal genetic contributions of founders and random genetic drift in nonfounders, respectively. Further examination using gene dropping simulation was conducted to obtain information on survival of founder alleles. Unique founder alleles were dropped down along the actual pedigree with Monte Carlo procedure following Mendelian segregation rules, and generated genotypes of all the current live animals (612 959 heads). Pedigree records consisted of 2 075 188 animals was used for these analysis. The estimates of three effective numbers (N-ef, N-ge, and N-enf) decreased from 418.6 to 50.3, 86.6 to 7.3, and 109.2 to 8.5, respectively, during the period 1960-2000. The increasing differences between two kinds of genetic diversity indices derived from N-ge and N-ef showed that large part of the reduced diversity from 1980 was attributed to genetic drift caused by the intensive use of particular limited number of sires. In gene dropping analysis, probabilities of extinction of founder alleles were derived from their distributions of frequency in the current animals. Several founders showed low probabilities of allele extinction, irrespective of their relatively low genetic contributions. This suggests that these founders have lineages through which their alleles are surely transmitted to the current breed. The use of these founders as a strategy for recovering the genetic diversity was discussed.

  BLACKWELL VERLAG GMBH, Aug. 2004, JOURNAL OF ANIMAL BREEDING AND GENETICS, 121(4) (4), 242 - 252, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Pedigree analysis of genetic subdivision in a population of Japanese Black cattle

  HONDA Takeshi, NOMURA Tetsuro, YAMAGUCHI Yuki, MUKAI Fumio

  Dec. 2002, Anim. Sci. J., 73(6) (6), 445 - 452, English


• Gene dropping analysis of founder contributions in a closed Japanese black cattle population

  HONDA Takeshi, NOMURA Tetsuro, FUKUSHIMA Moriyuki, MUKAI Fumio

  Apr. 2002, Anim. Sci. J., 73(2) (2), 105 - 111, English


• Inbreeding and effective population size of Japanese Black cattle

  T Nomura, T Honda, F Mukai

  The objective of this research was to estimate the amount of inbreeding and effective population size of the Japanese Black breed using pedigree records from bulls and heifers registered between 1985 and 1997. Inbreeding was quantified by three F-statistics: actual inbreeding, inbreeding expected under random mating, and inbreeding due to population subdivision. During the period of 1985 to 1997, the inbreeding expected under random mating increased from 2.3% to 5.0%, whereas the increase of actual inbreeding was more gradual (from 4.7% to 5.4%). The inbreeding due to population subdivision decreased almost linearly and reached 0.5% in 1997, indicating that genetic subdivision of the Japanese Black cattle population has essentially disappeared The effective size of the breed was estimated from the increasing rate of inbreeding expected under random mating. In the earlier half of this period (1986 to 1990), the breed maintained an effective size of approximately 30. However, after 1991 the effective size sharply decreased and the harmonic mean between 1993 and 1997 was only 17.2. The main cause of this reduction of the effective size was considered to be the intensive use of a few prominent sires. To increase the effective size, an upper limit in the use of AI semen per sire should be imposed.

  AMER SOC ANIMAL SCIENCE, Feb. 2001, JOURNAL OF ANIMAL SCIENCE, 79(2) (2), 366 - 370, English

  [Refereed]

  Scientific journal

• A consideration on the sampling method for fecal microbiota studies in a cattle population

  Takeshi Honda, Ryoto Murata, Daisuke Tanaka, Itsuko Fukuda, Kenji Oyama

  Lead, May 2024, Kansai Journal of Animal Science, (181) (181), 7 - 11, Japanese

  [Refereed]

  Report scientific journal


• 但馬牛における遺伝的多様性の維持のための取り組みと致死相当量の推定

  本多健

  Lead, Jun. 2020, 畜産技術, (781) (781), 2 - 6, Japanese


• 黒毛和種の遺伝的多様性の評価と維持・回復のための方策

  NOMURA TETSURO, HONDA TAKESHI

  2012, 最新農業技術 畜産, 4, 127 - 136, Japanese

  Introduction other


• 黒毛和種の遺伝的多様性の評価と維持・回復のための方策

  NOMURA TETSURO, HONDA TAKESHI

  2011, 農業技術体系・畜産編追録, (30) (30), 154の32 - 154の41, Japanese

  Introduction other


• 黒毛和種の集団構造に関する遺伝学的分析

  HONDA TAKESHI

  全国和牛登録協会, 2009, 和牛, 60(3) (3), 4 - 10, Japanese

  Introduction other


• テントウムシ集団の遺伝学的研究 (バイオリージョナリズムに基礎をおく京都の自然と生活文化に関する調査およびその展開) (平成16年度共同研究プロジェクト研究成果報告)

  野村 哲郎, 飯田 智, 祝前 博明, 本多 健

  京都産業大学, Jul. 2005, 京都産業大学総合学術研究所所報, 3, 131 - 148, Japanese

• 捕獲した野生動物集団の遺伝的構成を維持するための新しい遺伝的管理法

  本多健, 大山憲二

  日本生態学会第69回全国大会, Mar. 2022


• 但馬牛の系統分類が長期的な遺伝的多様性に与える効果

  山田栞, 本多健, 小浜菜美子, 坂瀬充洋, 大山憲二

  日本畜産学会大会講演要旨, 2019


• 黒毛和種で発生する白斑と枝肉形質との関連性の調査

  小山秀美, 有木 啓, 今村清人, 坂元信一, 大里和弘, 西 和隆, 河邊弘太郎, 岡本 新, 本多健, OYAMA KENJI, 下桐 猛

  日本動物遺伝育種学会第18回大会, Sep. 2018, Japanese, Domestic conference

  Poster presentation


• ゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索

  安栖梨理, 井上麻美, 小林 栄治, 福島 護之, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

  中島彩華, 栖原功太郎, 小林 栄治, 福島 護之, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• ゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索

  中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

  Oral presentation


• PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle

  Kawaguchi F, kigoshi H, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle

  Kigoshi H, Matsumoto Y, Kawaguchi F, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Oyama K, Honda T, Mannen H, Sasazaki S

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• 黒毛和種の損徴に関する遺伝育種学的研究

  小山 秀美, 今村 清人, 坂元 信一, 西 和隆, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  日本畜産学会第122回大会, Mar. 2017, Japanese, 神戸大学, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種で発生する白斑に関する遺伝育種学的研究

  小山 秀美, 今村 清人, 坂元 信一, 西 和隆, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  日本動物遺伝育種学会第17回大会, Nov. 2016, Japanese, Domestic conference

  Poster presentation


• 鹿児島県産黒毛和種における死産と母牛の審査時形質との関連性

  小山 秀美, 西 和隆, 今村 清人, 坂元 信一, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  日本暖地畜産学会第9回山口大会, Oct. 2016, Japanese, Domestic conference

  Oral presentation


• Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima

  Oyama H, Nishi K, Imamura K, Sakamoto S, Kawabe K, Okamoto S, HONDA TAKESHI, OYAMA KENJI, Shimogiri T

  Proceedings of 17th AAAP Animal Science Congress, Aug. 2016, English, International conference

  Poster presentation


• Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle

  Ozaki H, Tamura T, Fukazawa K, Uemoto Y, Nishio M, Kobayashi E, Matsuhashi T, Maruyama S, Honda T, Oyama K, Sasazaki S, Mannen H

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black

  Kawaguchi F, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• ウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索

  井上麻美, 中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年英之, 笹崎晋史

  第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference

  Poster presentation


• 兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の長期的な有効性

  NAKATSUKA MACHI, HONDA TAKESHI, FUKUSHIMA MORIYUKI, OYAMA KENJI

  日本動物遺伝育種学会第16回大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析

  松本祐汰, 中島彩華, 上本 吉伸, 小林栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 笹崎晋史, 本多健, 大山憲二, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定

  深澤謙治, 笹崎晋史, 大山憲二, 本多健, 小林栄治, 福島 護之, 秋山 敬孝, 小浜 菜美子, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析

  田村朋子, 深澤謙治, 尾崎弘樹, 上本吉伸, 西尾元秀, 小林栄治, 松橋珠子, 丸山新, 本多健, 笹崎晋史, 大山憲二, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• GWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索

  中嶋翼, 中島彩華, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 放牧場の俯瞰動画データを用いた繁殖牛のインタラクション検出

  細見 洋司, 大川 剛直, 大山 憲二, 本多 健

  第62回情報システム研究会, May 2015, Japanese, 神戸大学, Domestic conference

  Oral presentation


• GPSを利用した放牧牛のインタラクション情報の取得とその分析

  松本 健吾, 大川 剛直, 大山 憲二, 本多 健

  第62回情報システム研究会, May 2015, Japanese, 神戸大学, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の有効性

  NAKATSUKA MACHI, HONDA TAKESHI, FUKUSHIMA MORIYUKI, OYAMA KENJI

  日本畜産学会, Mar. 2015, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• 黒毛和種における内臓疾病に関連した経済的損失

  INOUE KEIICHI, HONDA TAKESHI, OYAMA KENJI

  日本畜産学会, Mar. 2015, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• 見島ウシの集団構造に関する基礎的研究

  HIRAMOTO AGEHA, HONDA TAKESHI, 稲吉 洋裕, OYAMA KENJI

  日本畜産学会, Mar. 2015, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種の枝肉における瑕疵間の遺伝相関の推定

  西 和隆, 下桐 猛, 草野 昭徳, 坂元 信一, 城元 清巳, 河邊 弘太郎, 岡本 新, 本多 健, 大山 憲二

  日本畜産学会第118回大会, Mar. 2014, Japanese, つくば市, Domestic conference

  Oral presentation


• Searching for causal structures among internal diseases and carcass traits in Japanese Black cattle

  井上 慶一, Valente Brouno D, 本多 健, OYAMA KENJI, Rosa Guilherme J

  日本畜産学会第118回大会, Mar. 2014, English, つくば市, Domestic conference

  Poster presentation


• 兵庫系黒毛和種における育種集団毎の遺伝的多様性評価

  福島 護之, 本多 健, 小路 怜子, 小浜 菜美子, 秋山 敬孝, 坂瀬 充洋, 岡 章生, 大山 憲二

  日本畜産学会第117回大会, 2013, Japanese, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種を用いた瑕疵と枝肉6形質の遺伝相関及び表型相関

  西 和隆, 下桐 猛, 草野 昭徳, 坂元 信一, 城元 清巳, 河邊 弘太郎, 岡本 新, 本多 健, 大山 憲二

  2013年度日本暖地畜産学会, 2013, Japanese, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種の枝肉で発生する瑕疵に関する遺伝的パラメータの推定

  西 和隆, 下桐 猛, 草野 昭徳, 坂元 信一, 城元 清巳, 河邊 弘太郎, 岡本 新, 本多 健, 大山 憲二

  日本畜産学会第117回大会, 2013, Japanese, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団における系統内交配による遺伝的分化の可能性

  木綿 萌, 本多 健, 福島 護之, 大山 憲二

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 黒毛和種の肥育後期における胸最長筋および腰最長筋の脂肪酸組成の経時的変化

  小栗 佑介, 柳原 佳代子, 石田 孝史, 本多 健, 万年 英之, 小林 郁雄, 大山憲二

  第62回関西畜産学会大会, 2012, Japanese, Domestic conference

  Oral presentation


• 黒毛和種の肥育後期における腰最長筋の筋肉内脂肪および脂肪酸組成の経時的変化

  小栗 佑介, 水野 雄仁, 本多 健, 万年 英之, 石田 孝史, 祝前 博明, 久下 志朗, 齋藤 邦彦, 大山 憲二

  日本畜産学会第114回大会, 2011, Japanese, Domestic conference

  Poster presentation


• 中立遺伝子頻度を推定するための血統情報を用いた個体の抽出方法

  HONDA TAKESHI, NOMURA TETSURO

  日本畜産学会第112回大会, 2010, Japanese, 東京, Domestic conference

  Oral presentation


• 黒毛和種の遺伝的多様性に関する研究

  HONDA TAKESHI

  日本畜産学会若手企画シンポジウム，日本畜産学会第112回大会奨励賞受賞記念ポスター講演, 2010, Japanese, 東京, Domestic conference

  [Invited]

  Invited oral presentation


• Sampling method for estimating neutral allele frequency in a pedigreed population

  HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, NOMURA Tetsuro, MUKAI Fumio

  9th World Congress of Genetics Applied to Livestock Production, 2010, English, International conference

  Poster presentation


• 黒毛和種繁殖雌牛の近年の泌乳量

  辻 絵美, 本多 健, 久下 志朗, 大山 憲二

  日本畜産学会第111回大会, 2009, Japanese, Domestic conference

  Oral presentation


• SNPsを活用した黒毛和種の親子鑑定の精度に関する検討

  HONDA TAKESHI, KATSUTA TOMOHIRO, MUKAI FUMIO

  日本畜産学会第110回大会, 2009, Japanese, 神奈川, Domestic conference

  Oral presentation


• 黒毛和種種雄牛の遺伝的関連性に関する研究

  本多 健, 藤井 俊英, 穴田 勝人, 向井 文雄

  関西畜産学会第55回大会, Sep. 2005, Japanese, 関西畜産学会, Ehime, Domestic conference

  Oral presentation


• 黒毛和種の遺伝的多様性の維持に寄与する分集団の評価

  本多 健, 出野 直樹, 野村 哲郎, 向井 文雄

  日本畜産学会第105回大会, Sep. 2005, Japanese, 日本畜産学会, Hokkaido, Domestic conference

  Poster presentation


• 褐毛和種の集団構造と遺伝的多様性に関する研究

  藤井 俊英, 本多 健, 野村 哲郎, 向井 文雄

  日本畜産学会第105回大会, Sep. 2005, Japanese, 日本畜産学会, Hokkaido, Domestic conference

  Poster presentation


• 複数の育種集団を用いた黒毛和種集団の遺伝的多様性の維持および近交係数の抑制

  本多 健, 野村 哲郎, 向井 文雄

  日本畜産学会第104 回大会, Mar. 2005, Japanese, 日本畜産学会, Tokyo, Domestic conference

  Oral presentation


• 黒毛和種の遺伝的多様性維持に際して優先すべき分集団の特定

  野村 哲郎, 本多 健, 出野 直樹, 向井 文雄

  第102回日本畜産学会, 2003, Japanese, 日本畜産学会, 岐阜大学, Domestic conference

  Oral presentation


• 黒毛和種の遺伝的多様性の現状と課題

  本多 健

  第41回家畜育種研究会, 2003, Japanese, 未記入, 未記入, Domestic conference

  Oral presentation


• 近交係数および遺伝的多様性からにみた育種集団の構造上の留意点

  本多 健, 野村 哲郎, 向井 文雄

  第102回日本畜産学会, 2003, Japanese, 日本畜産学会, 岐阜大学, Domestic conference

  Oral presentation

• 日本畜産学会

• ウシ体脂肪の脂肪酸組成に関る責任遺伝子群の総合的解明

  万年 英之

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2020

  Competitive research funding


• 遺伝子発現量を基盤とした黒毛和種の不飽和脂肪酸に関与する遺伝子の探索

  大山 憲二

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015

  Competitive research funding


• 国際標準ＳＮＰシステムの構築に基づくアジア在来家畜集団の遺伝的多様性解析と保全策

  万年 英之

  科学研究費補助金／基盤研究(B), 2011

  Competitive research funding


• 黒毛和種の遺伝的多様性の評価と維持システムに関する研究

  本多 健

  日本学術振興会, 科学研究費助成事業, 特別研究員奨励費, 神戸大学, 2003 - 2004

  本年度は,黒毛和種の遺伝的多様性の維持・回復のため,品種の遺伝子給源となる種牛供給系統群と,その遺伝子を導入する繁殖雌集団からなる機能的階層構造を想定し,系統群を維持・管理する上で留意すべき以下の2点について理論的検討を行った. 1.繁殖雌集団の近交係数の抑制 2.系統内の近交係数の抑制と系統群全体および系統間における遺伝的多様性の維持 検討事項1に対しては,輪番交配をモデルとして採用し,系統群の集団構造を変量とした雌集団の近交係数の漸化式を導出した.数値計算からは,世代当たり数頭の雄からなる4,5個の系統を確保できれば,系統内の有効サイズの大きさに関わらず,雌集団の近交係数を低いレベルに抑制できることを明らかにした. 検討事項2に対しては,中国5県において系統が造成・維持されている分集団構造をモデル化し,系統群の集団構造の影響について検討を加えた.また,家畜育種学および保全生物学における知見を基に,系統内の近交係数の上昇率,10世代後の系統群全体および系統間の遺伝的多様性指数に目標値を設定し,それらを満たす最適な系統内の雄の数(N_m)と系統間における雄の交換率(d_m)の探索を行った.諸条件から導かれた各目標値の変化を総合的に勘案した結果,N_m=10およびd_m=0.1が最適値であると結論付けた. 系統間における雄の交換は雌集団の近交係数を増加させる危険性があるため,本研究では上述した2つのモデルを融合させ,雌集団の近交係数の漸化式を再導出し,検討事項1について再度検討を行った.数値計算の結果,系統間で一定の割合で雄の交換が行われていても,4,5個の系統が確保できれば,輪番交配は雌集団の近交抑制に有効であることを明らかにした.さらに,上述した最適値に基づいて,中国5県集団を基礎とする5系統を維持すれば,同品種の近交係数は無視し得るほど低いレベルにまで抑制できることを示した.