Research activity information

• Jun. 2022 一般社団法人日本質量分析学会, MSSJ AWARD for Distinguished Service

  Ken-ichi YOSHINO

  Others

  IRI


• May 2006 The Mass Spectrometry Society of Japan, JMSSJ Award for Exellent Article, J. Mass Spectrom. Soc. Jpn. 52, 106-129 (2004)

  Ken-ichi Yoshino, Noriko Oshiro, Chaharu Tokunaga, Kazuyoshi Yonezawa

  IRI


• May 2003 社団法人日本肝臓学会, AJINOMOTO Award

  Ken-ichi YOSHINO


• Oct. 1988 日本動物学会, Zoological Science Award

  Norio Suzuki, Masanori Kurita, Ken-ichi Yoshino, Hiroko Kajiura, Koji Nomura, Masaaki Yamaguchi

• Skeleton binding protein 1 localizes to the Maurer's cleft and interacts with PfHSP70-1 and PfHSP70-x in Plasmodium falciparum gametocyte-infected erythrocytes

  Ayaka Omoda, Konomi Matsumoto, Ken-ichi Yoshino, Mayumi Tachibana, Takafumi Tsuboi, Motomi Torii, Tomoko Ishino, Hideyuki Iriko

  Elsevier BV, Feb. 2024, Parasitology International, 100, 102864 - 102864, English, International magazine

  [Refereed]

  Scientific journal


• Polo-like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5

  Shuji Ueda, Moeka Nishihara, Yuuki Hioka, Ken-ichi Yoshino, Soichiro Yamada, Minoru Yamanoue, Yasuhito Shirai

  Protein homeostasis, including protein folding, refolding, and degradation, is thought to decline with aging. HSPB5 (also known as αB-crystallin) prevents target protein aggregation as a molecular chaperone and exhibits a cytoprotective function against various cell stresses. To elucidate the effect of HSPB5 on endoplasmic reticulum (ER) stress, we searched for novel binding proteins of HSPB5 using the proximity-dependent biotin labeling method. Proteins presumed to interact with HSPB5 in cells treated with the proteasome inhibitor MG132 were identified by a reversible biotin-binding capacity method combining tamavidin2-REV magnetic beads and mass spectrometry. We discovered a new binding protein for HSPB5, polo-like kinase 2 (PLK2), which is an apoptosis-related enzyme. The expression of PLK2 was upregulated by MG132 treatment, and it was co-localized with HSPB5 near the ER in L6 muscle cells. Inhibition of PLK2 decreased ER stress-induced phosphorylation of serine 19 in HSPB5 and increased apoptosis by activation of caspase 3 under ER stress. Overexpression of HSPB5 (WT) suppressed the ER stress-induced caspase 3 activity, but this was not observed with phospho-deficient HSPB5 (3A) mutants. These results clarify the role of HSPB5 phosphorylation during ER stress and suggest that the PLK2/HSPB5 pathway plays an essential role in cytoprotection against proteasome inhibition-induced ER stress.

  Sep. 2022, Int. J. Mol. Sci., 23(19) (19), 11257, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gene Expression Analysis Provides New Insights into the Mechanism of Intramuscular Fat Formation in Japanese Black Cattle

  Shuji Ueda, Mana Hosoda, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai

  Japanese Black cattle (Japanese Wagyu) have a unique phenotype in which ectopic intramuscular fat accumulates in skeletal muscle, producing finely marbled beef. However, the mechanism of intramuscular fat formation in Japanese Black cattle remains unclear. To investigate the key genes involved in intramuscular fat accumulation, we comprehensively analyzed mRNA levels in subcutaneous and intramuscular fat tissues using RNA sequence (RNA-seq) analysis, which detected 27,606 genes. We identified eight key genes, namely carboxypeptidase E, tenascin C, transgelin, collagen type IV alpha 5 (COL4A5), cysteine and glycine-rich protein 2, PDZ, and LIM domain 3, phosphatase 1 regulatory inhibitor subunit 14A, and regulator of calcineurin 2. These genes were highly and specifically expressed in intramuscular fat tissue. Immunohistochemical analysis revealed a collagen network, including COL4A5, in the basement membrane around the intramuscular fat tissue. Moreover, pathway analysis revealed that, in intramuscular fat tissue, differentially expressed genes are related to cell adhesion, proliferation, and cancer pathways. Furthermore, pathway analysis showed that the transforming growth factor-β (TGF-β) and small GTPases regulators RASGRP3, ARHGEF26, ARHGAP10, ARHGAP24, and DLC were upregulated in intramuscular fat. Our study suggests that these genes are involved in intramuscular fat formation in Japanese Black cattle.

  MDPI AG, Jul. 2021, Genes, 12(8) (8), 1107, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Strategy for Protein Identification by Using Spectral Libraries of Chemically Modified Trypsin Autolysis Peptide Fragments

  Ken-ichi Yoshino, Itaru Usami, Atsuko Takeuchi

  The Mass Spectrometry Society of Japan, Feb. 2021, Journal of the Mass Spectrometry Society of Japan, 69(1) (1), 8 - 19, Japanese

  [Refereed]

  Scientific journal


• The C-terminal flexible region of branched-chain polyamine synthase facilitates substrate specificity and catalysis.

  Ryota Hidese, Masataka Toyoda, Ken-ichi Yoshino, Wakao Fukuda, Gita Adhirani Wihardja, Seigo Kimura, Junso Fujita, Masaru Niitsu, Tairo Oshima, Tadayuki Imanaka, Eiichi Mizohata, Shinsuke Fujiwara

  Branched-chain polyamine synthase (BpsA) catalyzes sequential aminopropyl transfer from the donor, decarboxylated S-adenosylmethionine (dcSAM), to the acceptor, linear-chain polyamine, resulting in the production of a quaternary-branched polyamine via tertiary branched polyamine intermediates. Here, we analyzed the catalytic properties and X-ray crystal structure of Tth-BpsA from Thermus thermophilus and compared them with those of Tk-BpsA from Thermococcus kodakarensis, which revealed differences in acceptor substrate specificity and C-terminal structure between these two enzymes. To investigate the role of the C-terminal flexible region in acceptor recognition, a region (QDEEATTY) in Tth-BpsA was replaced with that in Tk-BpsA (YDDEESSTT) to create chimeric Tth-BpsA C9, which showed a severe reduction in catalytic efficiency toward N4 -aminopropylnorspermidine, but not toward N4 -aminopropylspermidine, mimicking Tk-BpsA substrate specificity. Tth-BpsA C9 Tyr346 and Thr354 contributed to discrimination between tertiary branched-chain polyamine substrates, suggesting that the C-terminal region of BpsA recognizes acceptor substrates. Liquid chromatography-tandem mass spectrometry analysis on a Tk-BpsA reaction mixture with dcSAM revealed two aminopropyl groups bound to two of five aspartate/glutamate residues (Glu339 , Asp342 , Asp343 , Glu344 , and Glu345 ) in the C-terminal flexible region. Mutating each of these five amino acid residues to asparagine/glutamine resulted in a slight decrease in activity. The quadruple mutant D342N/D343N/E344Q/E345Q exhibited a severe reduction in catalytic efficiency, suggesting that these aspartate/glutamate residues function to receive aminopropyl chains. In addition, the X-ray crystal structure of the Tk-BpsA ternary complex bound to N4 -bis(aminopropyl)spermidine revealed that Asp126 and Glu259 interacted with the aminopropyl moiety in N4 -aminopropylspermidine.

  Oct. 2019, The FEBS Journal, 286(19) (19), 3926 - 3940, English, International magazine

  [Refereed]

  Scientific journal


• The Role of Cysteine String Protein α Phosphorylation at Serine 10 and 34 by Protein Kinase Cγ for Presynaptic Maintenance.

  Toshihiko Shirafuji, Takehiko Ueyama, Naoko Adachi, Ken-ichi Yoshino, Yusuke Sotomaru, Junsuke Uwada, Azumi Kaneoka, Taro Ueda, Shigeru Tanaka, Izumi Hide, Naoaki Saito, Norio Sakai

  Society for Neuroscience, Jan. 2018, The Journal of Neuroscience, 38(2) (2), 278 - 290, English, International magazine

  [Refereed]

  Scientific journal


• Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.

  Caitlin Kozel, Brytteny Thompson, Samantha Hustak, Chelsea Moore, Akio Nakashima, Chingakham Ranjit Singh, Megan Reid, Christian Cox, Evangelos Papadopoulos, Rafael E Luna, Abbey Anderson, Hideaki Tagami, Hiroyuki Hiraishi, Emily Archer Slone, Ken-ichi Yoshino, Masayo Asano, Sarah Gillaspie, Jerome Nietfeld, Jean-Pierre Perchellet, Stefan Rothenburg, Hisao Masai, Gerhard Wagner, Alexander Beeser, Ushio Kikkawa, Sherry D Fleming, Katsura Asano

  Oct. 2016, Nucleic acids research, 44(18) (18), 8704 - 8713, English, International magazine

  [Refereed]

  Scientific journal


• Hepatitis C virus NS5A protein interacts with lysine methyltransferase SET and MYND domain-containing 3 and induces activator protein 1 activation.

  Ming Chen, Xiang Gan, Ken-ichi Yoshino, Madoka Kitakawa, Ikuo Shoji, Lin Deng, Hak Hotta

  Jun. 2016, Microbiology and immunology, 60(6) (6), 407 - 417, English, International magazine

  [Refereed]

  Scientific journal


• Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein.

  Syota Matsumoto, Eric S Fischer, Takeshi Yasuda, Naoshi Dohmae, Shigenori Iwai, Toshio Mori, Ryotaro Nishi, Ken-ichi Yoshino, Wataru Sakai, Fumio Hanaoka, Nicolas H Thomä, Kaoru Sugasawa

  In mammalian nucleotide excision repair, the DDB1-DDB2 complex recognizes UV-induced DNA photolesions and facilitates recruitment of the XPC complex. Upon binding to damaged DNA, the Cullin 4 ubiquitin ligase associated with DDB1-DDB2 is activated and ubiquitinates DDB2 and XPC. The structurally disordered N-terminal tail of DDB2 contains seven lysines identified as major sites for ubiquitination that target the protein for proteasomal degradation; however, the precise biological functions of these modifications remained unknown. By exogenous expression of mutant DDB2 proteins in normal human fibroblasts, here we show that the N-terminal tail of DDB2 is involved in regulation of cellular responses to UV. By striking contrast with behaviors of exogenous DDB2, the endogenous DDB2 protein was stabilized even after UV irradiation as a function of the XPC expression level. Furthermore, XPC competitively suppressed ubiquitination of DDB2 in vitro, and this effect was significantly promoted by centrin-2, which augments the DNA damage-recognition activity of XPC. Based on these findings, we propose that in cells exposed to UV, DDB2 is protected by XPC from ubiquitination and degradation in a stochastic manner; thus XPC allows DDB2 to initiate multiple rounds of repair events, thereby contributing to the persistence of cellular DNA repair capacity.

  Feb. 2015, Nucleic acids research, 43(3) (3), 1700 - 1713, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Chicken heat shock protein HSPB1 increases and interacts with αB-crystallin in aged skeletal muscle.

  Shuji Ueda, Yoshito Kokaji, Shunsaku Simizu, Kazuhisa Honda, Ken-ichi Yoshino, Hiroshi Kamisoyama, Yasuhito Shirai, Minoru Yamanoue

  2015, Bioscience, biotechnology, and biochemistry, 79(11) (11), 1867 - 1875, English, International magazine

  [Refereed]

  Scientific journal


• The role of Pak-interacting exchange factor-β phosphorylation at serines 340 and 583 by PKCγ in dopamine release.

  Toshihiko Shirafuji, Takehiko Ueyama, Ken-ichi Yoshino, Hideyuki Takahashi, Naoko Adachi, Yukio Ago, Ken Koda, Tetsuaki Nashida, Naoki Hiramatsu, Toshio Matsuda, Tatsushi Toda, Norio Sakai, Naoaki Saito

  Jul. 2014, The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(28) (28), 9268 - 9280, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes.

  Akio Nakashima, Ippei Kawanishi, Sumiko Eguchi, Eugene Hsin Yu, Satoshi Eguchi, Noriko Oshiro, Ken-ichi Yoshino, Ushio Kikkawa, Kazuyoshi Yonezawa

  Apr. 2013, Journal of biomedical science, 20, 24 - 24, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Novel PKCα-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release.

  Megumi Sakuma, Yasuhito Shirai, Ken-ichi Yoshino, Maho Kuramasu, Tomofumi Nakamura, Toshihiko Yanagita, Kensaku Mizuno, Izumi Hide, Yoshihiro Nakata, Naoaki Saito

  Sep. 2012, Molecular biology of the cell, 23(18) (18), 3707 - 3721, English, International magazine

  [Refereed]

  Scientific journal


• c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr-218.

  Takehiro Matsubara, Momo Ikeda, Yuko Kiso, Megumi Sakuma, Ken-ichi Yoshino, Fumio Sakane, Isabel Merida, Naoaki Saito, Yasuhito Shirai

  Feb. 2012, The Journal of biological chemistry, 287(8) (8), 5507 - 5517, English, International magazine

  [Refereed]

  Scientific journal


• Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron.

  Seki T, Yoshino K, Tanaka S, Dohi E, Onji T, Yamamoto K, Hide I, Paulson HL, Saito N, Sakai N

  BACKGROUND: Chaperone-mediated autophagy (CMA) is a selective autophagy-lysosome protein degradation pathway. The role of CMA in normal neuronal functions and in neural disease pathogenesis remains unclear, in part because there is no available method to monitor CMA activity at the single-cell level. METHODOLOGY/PRINCIPAL FINDINGS: We sought to establish a single-cell monitoring method by visualizing translocation of CMA substrates from the cytosol to lysosomes using the HaloTag (HT) system. GAPDH, a CMA substrate, was fused to HT (GAPDH-HT); this protein accumulated in the lysosomes of HeLa cells and cultured cerebellar Purkinje cells (PCs) after labeling with fluorescent dye-conjugated HT ligand. Lysosomal accumulation was enhanced by treatments that activate CMA and prevented by siRNA-mediated knockdown of LAMP2A, a lysosomal receptor for CMA, and by treatments that inactivate CMA. These results suggest that lysosomal accumulation of GAPDH-HT reflects CMA activity. Using this method, we revealed that mutant γPKC, which causes spinocerebellar ataxia type 14, decreased CMA activity in cultured PCs. CONCLUSION/SIGNIFICANCE: In the present study, we established a novel fluorescent-based method to evaluate CMA activity in a single neuron. This novel method should be useful and valuable for evaluating the role of CMA in various neuronal functions and neural disease pathogenesis.

  2012, PloS one, 7(2) (2), e31232, English, International magazine

  [Refereed]

  Scientific journal


• Direct binding of RalA to PKCη and its crucial role in morphological change during keratinocyte differentiation.

  Yasuhito Shirai, Shoko Morioka, Megumi Sakuma, Ken-ichi Yoshino, Chihiro Otsuji, Norio Sakai, Kaori Kashiwagi, Kazuhiro Chida, Ryutaro Shirakawa, Hisanori Horiuchi, Chikako Nishigori, Takehiko Ueyama, Naoaki Saito

  Apr. 2011, Molecular biology of the cell, 22(8) (8), 1340 - 52, English, International magazine

  [Refereed]

  Scientific journal


• Tor directly controls the Atg1 kinase complex to regulate autophagy.

  Yoshiaki Kamada, Ken-ichi Yoshino, Chika Kondo, Tomoko Kawamata, Noriko Oshiro, Kazuyoshi Yonezawa, Yoshinori Ohsumi

  Feb. 2010, Molecular and cellular biology, 30(4) (4), 1049 - 58, English, International magazine

  [Refereed]

  Scientific journal


• Precursor ion scanning and sequencing of arginine-ADP-ribosylated peptide by mass spectrometry.

  Harumi Osago, Kazuo Yamada, Tomoko Shibata, Ken-ichi Yoshino, Nobumasa Hara, Mikako Tsuchiya

  Oct. 2009, Analytical biochemistry, 393(2) (2), 248 - 54, English, International magazine

  [Refereed]

  Scientific journal


• AMP-activated protein kinase phosphorylates glutamine : fructose-6-phosphate amidotransferase 1 at Ser243 to modulate its enzymatic activity.

  Satoshi Eguchi, Noriko Oshiro, Takafumi Miyamoto, Ken-ichi Yoshino, Sumiko Okamoto, Takamasa Ono, Ushio Kikkawa, Kazuyoshi Yonezawa

  Feb. 2009, Genes to cells : devoted to molecular & cellular mechanisms, 14(2) (2), 179 - 89, English, International magazine

  [Refereed]

  Scientific journal


• AMP-activated protein kinase phosphorylates Golgi-specific brefeldin A resistance factor 1 at Thr1337 to induce disassembly of Golgi apparatus.

  Takafumi Miyamoto, Noriko Oshiro, Ken-ichi Yoshino, Akio Nakashima, Satoshi Eguchi, Mikiko Takahashi, Yoshitaka Ono, Ushio Kikkawa, Kazuyoshi Yonezawa

  Feb. 2008, The Journal of Biological Chemistry, 283(7) (7), 4430 - 4438, English, International magazine

  [Refereed]

  Scientific journal


• Identification of TBC7 having TBC domain as a novel binding protein to TSC1-TSC2 complex.

  Akio Nakashima, Ken-ichi Yoshino, Takafumi Miyamoto, Satoshi Eguchi, Noriko Oshiro, Ushio Kikkawa, Kazuyoshi Yonezawa

  Sep. 2007, Biochemical and biophysical research communications, 361(1) (1), 218 - 23, English, International magazine

  [Refereed]

  Scientific journal


• The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1.

  Noriko Oshiro, Rinako Takahashi, Ken-ichi Yoshino, Keiko Tanimura, Akio Nakashima, Satoshi Eguchi, Takafumi Miyamoto, Kenta Hara, Kenji Takehana, Joseph Avruch, Ushio Kikkawa, Kazuyoshi Yonezawa

  Jul. 2007, The Journal of biological chemistry, 282(28) (28), 20329 - 39, English, International magazine

  [Refereed]

  Scientific journal


• Phosphorylation and up-regulation of diacylglycerol kinase gamma via its interaction with protein kinase C gamma.

  Yasuto Yamaguchi, Yasuhito Shirai, Takehiro Matsubara, Koichi Sanse, Masamitsu Kuriyama, Noriko Oshiro, Ken-ichi Yoshino, Kazuyoshi Yonezawa, Yoshitaka Ono, Naoaki Saito

  Oct. 2006, The Journal of biological chemistry, 281(42) (42), 31627 - 37, English, International magazine

  [Refereed]

  Scientific journal


• Different roles for the TOS and RAIP motifs of the translational regulator protein 4E-BP1 in the association with raptor and phosphorylation by mTOR in the regulation of cell size.

  Satoshi Eguchi, Chiharu Tokunaga, Sujuti Hidayat, Noriko Oshiro, Ken-ichi Yoshino, Ushio Kikkawa, Kazuyoshi Yonezawa

  Jul. 2006, Genes to cells : devoted to molecular & cellular mechanisms, 11(7) (7), 757 - 66, English, International magazine

  [Refereed]

  Scientific journal


• Phylogeny of vertebrate Src tyrosine kinases revealed by the epitope region of mAb327.

  Tetsushi Iwasaki, Ken-ichi Sato, Ken-ichi Yoshino, Shuji Itakura, Keiko Kosuge, Alexander A Tokmakov, Koji Owada, Kazuyoshi Yonezawa, Yasuo Fukami

  Mar. 2006, Journal of Biochemistry, 139(3) (3), 347 - 354, English, International magazine

  [Refereed]

  Scientific journal


• Suppression of the mTOR-raptor signaling pathway by the inhibitor of heat shock protein 90 geldanamycin.

  Goh Ohji, Sujuti Hidayat, Akio Nakashima, Chiharu Tokunaga, Noriko Oshiro, Ken-Ichi Yoshino, Koichi Yokono, Ushio Kikkawa, Kazuyoshi Yonezawa

  Jan. 2006, Journal of biochemistry, 139(1) (1), 129 - 135, English, International magazine

  [Refereed]

  Scientific journal


• Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa.

  Xiang Gan, Kyoko Arita, Setsuko Isono, Madoka Kitakawa, Ken-ichi Yoshino, Kazuyoshi Yonezawa, Akihiro Kato, Hirokazu Inoue, Katsumi Isono

  Jan. 2006, FEMS microbiology letters, 254(1) (1), 157 - 64, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Biochemical and morphologic evidence of the interaction of oligodendrocyte membrane rafts with actin filaments.

  Katsutoshi Taguchi, Koji Yoshinaka, Ken-ichi Yoshino, Kazuyoshi Yonezawa, Shohei Maekawa

  Cytoskeletal structures under the cell membrane carry out pivotal roles in the maintenance and remodeling of the cell structures. Reforming of the cytoskeletal networks after partial extraction of membrane components could be a good clue to identify molecular components pertaining the interaction of cytoskeleton with membrane. A detergent extract from 3-week-old rat brain membrane fraction was found to make an actin-based gel upon incubation at 25 degrees C. Some protein components of the gelation products were recovered in a Triton-insoluble low-density microdomain fraction (raft) after depolymerization of actin filaments. Some of these proteins were identified as 2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase), proteolipid protein (PLP), and myelin oligodendrocyte glycoprotein (MOG) through electrospray time-of-flight (ESI-TOF) analysis and Western blotting. Because these proteins are well-known marker proteins of oligodendrocytes, localization of these proteins and cholesterol, a raft-localized lipid, with actin filaments was studied using cultured oligodendrocytes. Clear colocalization of these proteins and cholesterol with actin filaments was observed after Triton treatment at 4 degrees C before fixation. These results indicate that raft microdomains participate in the formation of cell shape through interaction with microfilaments in oligodendrocytes.

  Jul. 2005, Journal of neuroscience research, 81(2) (2), 218 - 225, English, International magazine

  [Refereed]

  Scientific journal


• Molecular identification and characterization of Xenopus egg uroplakin III, an egg raft-associated transmembrane protein that is tyrosine-phosphorylated upon fertilization.

  Keiichi Sakakibara, Ken-ichi Sato, Ken-ichi Yoshino, Noriko Oshiro, Shino Hirahara, A K M Mahbub Hasan, Tetsushi Iwasaki, Yasushi Ueda, Yasuhiro Iwao, Kazuyoshi Yonezawa, Yasuo Fukami

  Apr. 2005, The Journal of biological chemistry, 280(15) (15), 15029 - 37, English, International magazine

  [Refereed]

  Scientific journal


• Involvement of General Transcriptional Coactivator PC4 in the Transcription of Medaka Fish Intestine-Specific Membrane Guanylyl Cyclase Gene (OlGC6)

  NAKAUCHI Mina, YOSHINO Ken-ichi, YONEZAWA Kazuyoshi, SUZUKI Norio

  Apr. 2005, Journal of Biochemistry, 137(4) (4), 509 - 515, English, International magazine

  [Refereed]


• Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function.

  Noriko Oshiro, Ken-ichi Yoshino, Sujuti Hidayat, Chiharu Tokunaga, Kenta Hara, Satoshi Eguchi, Joseph Avruch, Kazuyoshi Yonezawa

  The mammalian target of rapamycin (mTOR) is a Ser/Thr protein kinase that plays a crucial role in a nutrient-sensitive signalling pathway that regulates cell growth. TOR signalling is potently inhibited by rapamycin, through the direct binding of a FK506-binding protein 12 (FKBP12)/rapamycin complex to the TOR FRB domain, a segment amino terminal to the kinase catalytic domain. The molecular basis for the inhibitory action of FKBP12/rapamycin remains uncertain. Raptor (regulatory associated protein of mTOR) is a recently identified mTOR binding partner that is essential for mTOR signalling in vivo, and whose binding to mTOR is critical for mTOR-catalysed substrate phosphorylation in vitro. Here we investigated the stability of endogenous mTOR/raptor complex in response to rapamycin in vivo, and to the direct addition of a FKBP12/rapamycin complex in vitro. Rapamycin diminished the recovery of endogenous raptor with endogenous or recombinant mTOR in vivo; this inhibition required the ability of mTOR to bind the FKBP12/rapamycin complex, but was independent of mTOR kinase activity. Rapamycin, in the presence of FKBP12, inhibited the association of raptor with mTOR directly in vitro, and concomitantly reduced the mTOR-catalysed phosphorylation of raptor-dependent, but not raptor-independent substrates; mTOR autophosphorylation was unaltered. These observations indicate that rapamycin inhibits mTOR function, at least in part, by inhibiting the interaction of raptor with mTOR; this action uncouples mTOR from its substrates, and inhibits mTOR signalling without altering mTOR's intrinsic catalytic activity.

  Apr. 2004, Genes to cells : devoted to molecular & cellular mechanisms, 9(4) (4), 359 - 366, English, International magazine

  [Refereed]

  Scientific journal


• Protein-tyrosine phosphatase alpha, RPTP alpha, is a Helicobacter pylori VacA receptor.

  Kinnosuke Yahiro, Akihiro Wada, Masaaki Nakayama, Takahiro Kimura, Ken-ichi Ogushi, Takuro Niidome, Haruhiko Aoyagi, Ken-ichi Yoshino, Kazuyoshi Yonezawa, Joel Moss, Toshiya Hirayama

  Helicobacter pylori vacuolating cytotoxin, VacA, induces vacuolation, mitochondrial damage, cytochrome c release, and apoptosis of gastric epithelial cells. To detect gastric proteins that serve as VacA receptors, we used VacA co-immunoprecipitation techniques following biotinylation of the cell surface and identified p250, a receptor-like protein-tyrosine phosphatase beta (RPTP beta) as a VacA-binding protein (Yahiro, K., Niidome, T., Kimura, M., Hatakeyama, T., Aoyagi, H., Kurazono, H., Imagawa, K., Wada, A., Moss, J., and Hirayama, T. (1999) J. Biol. Chem. 274, 36693-36699). VacA causes vacuolation of G401 cells, a human kidney tumor cell line, although they do not express RPTP beta. By co-immunoprecipitation with VacA, we identified p140 as a potential receptor in those cells. p140 purified by chromatography on a peanut agglutinin affinity matrix contained internal amino acid sequences of RGEENTDYVNASFIDGYRQK and AEGILDVFQTVK, which are identical to those in RPTP alpha. The peptide mass fingerprinting of p140 by time of flight-MS analysis also supported this identification. Treatment of G401 cells with RPTP alpha-morpholino antisense oligonucleotide before exposure to toxin inhibited vacuolation. These data suggest that RPTP alpha acts as a receptor for VacA in G401 cells. Thus, two receptor tyrosine phosphatases, RPTP alpha and RPTP beta, serve as VacA receptors.

  May 2003, The Journal of biological chemistry, 278(21) (21), 19183 - 19189, English, International magazine

  [Refereed]

  Scientific journal


• The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.

  Hiroki Nojima, Chiharu Tokunaga, Satoshi Eguchi, Noriko Oshiro, Sujuti Hidayat, Ken-ichi Yoshino, Kenta Hara, Noriaki Tanaka, Joseph Avruch, Kazuyoshi Yonezawa

  May 2003, The Journal of biological chemistry, 278(18) (18), 15461 - 4, English, International magazine

  [Refereed]

  Scientific journal


• Inhibition of amino acid-mTOR signaling by a leucine derivative induces G1 arrest in Jurkat cells.

  Sujuti Hidayat, Ken-ichi Yoshino, Chiharu Tokunaga, Kenta Hara, Masafumi Matsuo, Kazuyoshi Yonezawa

  We have previously demonstrated that N-acetylleucine amide, a derivative of L-leucine, inhibits leucine-induced p70(S6k) activation in a rat hepatoma cell line. In the present study, we investigated whether N-acetylleucine amide is capable of inhibiting amino acid-mTOR signaling. N-Acetylleucine amide caused cell cycle arrest at G1 stage in Jurkat cells, a human leukemia T cell line, concomitant with the inhibition of serum-induced p70(S6k) activation and p27 degradation. Treatment of Jurkat cells with this compound also exhibited dephosphorylation of retinoblastoma protein. These effects are similar to the inhibitory effects of rapamycin on amino acid-mTOR signaling pathway and suggest that N-acetylleucine amide acts as a rapamycin-like reagent to inhibit cell cycle progression in Jurkat cells.

  Feb. 2003, Biochemical and biophysical research communications, 301(2) (2), 417 - 423, English, International magazine

  [Refereed]

  Scientific journal


• A possible linkage between AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) signalling pathway.

  Naoki Kimura, Chiharu Tokunaga, Sushila Dalal, Christine Richardson, Ken-ichi Yoshino, Kenta Hara, Bruce E Kemp, Lee A Witters, Osamu Mimura, Kazuyoshi Yonezawa

  BACKGROUND: The mammalian target of rapamycin (mTOR) regulates multiple cellular functions including translation in response to nutrients, especially amino acids. AMP-activated protein kinase (AMPK) modulates metabolism in response to energy demand by responding to changes in AMP. RESULTS: The treatment of SV40-immortalized human corneal epithelial cells (HCE-T cells) with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), widely used as an AMPK activator, inhibits p70 S6k activities. Altered glucose availability, which regulates AMPK activity, also modulates the activity of p70 S6k. AICAR treatment also inhibits phosphorylation of Thr-412 in the p70 S6 kinase (p70 S6k), which is indispensable for the activity. Furthermore, over-expression of mutant AMPK subunits by stable expression in rabbit pulmonary fibroblast cell lines (PS120 cells) also modulates p70 S6k activity. The insensitivity of the rapamycin-resistant p70 S6k variant to AICAR treatment suggests that the inhibition of p70 S6k is mediated through a common effector, supporting a model whereby mTOR and its downstream effector are controlled by AMPK. CONCLUSION: These results indicate that the AMPK and mTOR signalling pathways are possibly linked. In addition to the mTOR signal acting as a priming switch that modulates p70 S6k activation, AMPK appears to provide an overriding switch linking p70 S6k regulation to cellular energy metabolism.

  Jan. 2003, Genes to Cells, 8(1) (1), 65 - 79, English, International magazine

  [Refereed]

  Scientific journal


• Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components.

  Xiang Gan, Madoka Kitakawa, Ken-ichi Yoshino, Noriko Oshiro, Kazuyoshi Yonezawa, Katsumi Isono

  Mitochondrial ribosomal proteins (mrps) of the budding yeast, Saccharomyces cerevisiae, have been extensively characterized genetically and biochemically. However, the list of the genes encoding individual mrps is still not complete and quite a few of the mrps are only predicted from their similarity to bacterial ribosomal proteins. We have constructed a yeast strain in which one of the small subunit proteins, termed Mrp4, was tagged with S-peptide and used for affinity purification of mitochondrial ribosome. Mass spectrometric analysis of the isolated proteins detected most of the small subunit mrps which were previously identified or predicted and about half of the large subunit mrps. In addition, several proteins of unknown function were identified. To confirm their identity further, we added tags to these proteins and analyzed their localization in subcellular fractions. Thus, we have newly established Ymr158w (MrpS8), Ypl013c (MrpS16), Ymr188c (MrpS17) and Ygr165w (MrpS35) as small subunit mrps and Img1, Img2, Ydr116c (MrpL1), Ynl177c (MrpL22), Ynr022c (MrpL50) and Ypr100w (MrpL51) as large subunit mrps.

  Nov. 2002, European Journal of Biochemistry, 269(21) (21), 5203 - 5214, English, International magazine

  [Refereed]

  Scientific journal


• Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.

  Kenta Hara, Yoshiko Maruki, Xiaomeng Long, Ken-ichi Yoshino, Noriko Oshiro, Sujuti Hidayat, Chiharu Tokunaga, Joseph Avruch, Kazuyoshi Yonezawa

  mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

  Jul. 2002, Cell, 110(2) (2), 177 - 189, English, International magazine

  [Refereed]

  Scientific journal


• Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.

  Takeshi Shimizu, Kensuke Shima, Ken-ichi Yoshino, Kazuyoshi Yonezawa, Tohru Shimizu, Hideo Hayashi

  The proteins under the control of the two-component system VirR/VirS in Clostridium perfringens were analyzed by using two-dimensional gel electrophoresis of the culture supernatant from the wild type and the virR mutant. Based on matrix-assisted laser desorption ionization-time of flight/mass spectrometry, seven positively regulated proteins and eight negatively regulated proteins were identified. Transcriptome analysis confirmed that 7 of the 15 proteins were regulated by the VirR/VirS system at the transcriptional level, but the remaining proteins were modified with a VirR/VirS-directed protease at the posttranslation and secretion levels. We purified and characterized the VirR/VirS-directed protease from the culture supernatant and identified it as a kind of clostripain. Because this proteolytic activity was strongly inhibited by leupeptin and antipain, it was concluded that this protease was a member of the family of cysteine proteases of C. perfringens.

  May 2002, Journal of bacteriology, 184(10) (10), 2587 - 2594, English, International magazine

  [Refereed]

  Scientific journal


• The complete amino acid sequence determination of a novel protein by mass spectrometry with the tentative human consensus sequence database

  Ken-Ichi Yoshino, Kenta Hara, Noriko Oshiro, Sujuti Hidayat, Chiharu Tokunaga, Kazuyoshi Yonezawa

  2002, Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 751 - 752, English

  International conference proceedings


• Distinct regulatory mechanism for p70 S6 kinase beta from that for p70 S6 kinase alpha.

  Taichi Minami, Kenta Hara, Noriko Oshiro, Sachiko Ueoku, Ken-ichi Yoshino, Chinaru Tokunaga, Yasuhito Shirai, Naoaki Saito, Ivan Gout, Kazuyoshi Yonezawa

  BACKGROUND: A novel ribosomal S6 kinase, termed p70 S6 kinase beta (p70beta), has a highly homologous amino acid sequence to that of p70/p85 S6 kinase (p70alpha). This includes the critical phosphorylation sites, Thr252, Ser394 and Thr412 in p70alpha1, which correspond to Thr241, Ser383 and Thr401 in p70beta1, respectively. However, the regulatory mechanism for p70beta remains to be elucidated. RESULTS: We report here the expression and the mechanism of in vivo regulation of p70beta. Two isoforms, p70beta1 and p70beta2, were expressed in a variety of tissues at a different level. p70beta1 was mainly targeted to the nucleus, whereas p70beta2 dispersed throughout the cytoplasm including nucleoplasm. The kinase activity of p70beta1 was less sensitive to the inhibition induced by rapamycin, wortmannin and amino acid withdrawal than that of p70alpha. The portion of p70beta activity inhibited by rapamycin was rescued by the rapamycin-resistant mutant of the mammalian target of rapamycin (mTOR). Mutational analysis revealed that the phosphorylation of Thr241 and Thr401 in p70beta1 was indispensable for the kinase activity. In contrast, a p70beta1 mutant in which Ser383 was substituted with Gly (S383G) still retained nearly the half maximal activity. Sequential phosphorylation of wild-type and S383G mutant of p70beta1 with mTOR and 3-phosphoinositide-dependent protein kinase 1 (PDK1) in vitro synergistically activated their kinase activities. CONCLUSION: These results indicate that p70beta is regulated by the mTOR- and PDK1-signalling pathways through a synergistic interaction between phosphorylated Thr241 and Thr401, while Ser383 plays minor role in their activation mechanism. Activated p70beta may be less sensitive to dephosphorylation mediated by putative phosphatases activated by rapamycin, amino acid withdrawal, and probably wortmannin.

  Nov. 2001, Genes to Cells, 6(11) (11), 1003 - 1015, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Carboxyl-terminal region conserved among phosphoinositide-kinase-related kinases is indispensable for mTOR functionin vivoandin vitro

  Tetsuya Takahashi, Kenta Hara, Hitomi Inoue, Yoshitaka Kawa, Chiharu Tokunaga, Sujuti Hidayat, Ken-ichi Yoshino, Yoshikazu Kuroda, Kazuyoshi Yonezawa

  Wiley, Sep. 2000, Genes to Cells, 5(9) (9), 765 - 775, English

  [Refereed]

  Scientific journal


• A case of Salmonellosis with severe neurological impairment caused by Salmonella urbana (O30), which antigen is identical to the Escherichia coli O157 antigen

  MINAMI Koichi, YANAGAWA Toshihiko, KOBAYASHI Masakazu, SUZUKI Hiroyuki, NAKANISHI Naoyuki, SHIMOYAMADA Yozo, OISHI Ko, YOSHINO Kenichi, TAKEDA Tae, KOIKE Michio

  Mar. 2000, 小児感染免疫, 12(1) (1), 19 - 22, Japanese

  [Refereed]

  Scientific journal


• In vitro assessment of a chemically synthesized Shiga toxin receptor analog attached to Chromosorb P (Synsorb Pk) as a specific absorbing agent of Shiga toxin 1 and 2

  Tae Takeda, Ken-ichi Yoshino, Eriko Adachi, Yuko Sato, Kyoko Yamagata

  1999, Microbiology and Immunology, 43(4) (4), 331 - 337, English

  [Refereed]

  Scientific journal


• Proteolysis of SNAP-25 isoforms by botulinum neurotoxin types A, C, and E: Domains and amino acid residues controlling the formation of enzyme- substrate complexes and cleavage

  Vadakkanchery V. Vaidyanathan, Ken-ichi Yoshino, Michael Jahnz, Christos Dörries, Steffen Bade, Sonja Nauenburg, Heiner Niemann, Thomas Binz

  1999, Journal of Neurochemistry, 72(1) (1), 327 - 337, English

  [Refereed]

  Scientific journal


• Anti-LPS Antibodies in Adult and Infant Patients Infected with Enterohemorrhagic Escherichia coli O157

  ADACHI Eriko, YOSHINO Ken-ichi, TAKEDA Tae

  The antibodies against LPS of enterohemorrhagic Escherichia coli (EHEC) O157 in sera from patients infected with this organism and sera from healthy control subjects were examined by ELISA to investigate their immune response. Analysing these data, we found some heterogeneity of antibody response between adults and infants.
  In healthy adults and children, the cutoff values for IgM antibodies to O157 LPS were calculated as 0.85, 0.40 and for IgG were as 0.57, 0.39 respectively. In patients, the ELISA values of adult were lower and decreased earlier than those of children. Therefore sero diagnosis for EHEC O157 infection for adult patients the timing of blood collection is very important. On the other hand, in some patients the sera of children showed a high level of IgM only 3 days from the onset. This tendency seemed to be related to severity of the disease. This immunological test was proved to be useless for healthy carriers because the antibodies against O157 LPS were not observed in their sera.

  The Japanese Association for Infectious Diseases, 1999, Kansenshogaku Zasshi, 73(8) (8), 772 - 777, Japanese

  [Refereed]

  Scientific journal


• 急激に重症脳症に進展したサルモネラ感染症の1例

  中西 直之, 南 弘一, 柳川 敏彦, 小林 昌和, 小池 通夫, 大石 興, 吉川 明男, 吉野 健一, 竹田 多恵

  日本小児感染症学会, Oct. 1998, 小児感染免疫, 10(3) (3), 227 - 227, Japanese, Domestic magazine

  [Refereed]

  Scientific journal


• Mass spectrometric analysis of phosphoserine residues conserved in the catalytic domain of membrane-bound guanylyl cyclase from the sea urchin spermatozoa

  Hirotaka Furuya, Ken-ichi Yoshino, Takeshi Shimizu, Tsuyoshi Mantoku, Tae Takeda, Kohji Nomura, Norio Suzuki

  Aug. 1998, Zoological Science, 15(4) (4), 507 - 516, English

  [Refereed]

  Scientific journal


• Anti-verotoxin-neutralizing antibody in intravenous gammaglobulin preparations

  E. Adachi, K. Yoshino, T. Kimura, Y. Matsumoto, T. Takeda

  The Japanese Association for Infectious Diseases, Aug. 1998, Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, 72(8) (8), 808 - 812, Japanese

  [Refereed]

  Scientific journal


• Evaluation of Immunochromatography-based Rapid Detection Kit for Fecal Escherichia coliO157

  TAKEDA Tae, YAMAGATA Kyoko, YOSHIDA Yuji, YOSHINO Kenichi, NOMURA Toshie

  “Quix^TM” is an immunochromato graphy-based direct detection kit for the E. coli O157 LPS antigen in the patient's stool. The present study was conducted to evaluate the efficacy of the kit for rapid diagnosis of enterohemorrhagic Escherichia coli (EHEC) O157 infection.
  Sensitivity of the kit was determined using a pure culture of a clinical isolate of E. coli O157. Analytical sensitivity was found to be 5×10⁵ CFU/ml. When compared with the culture method using fecal samples of 64 patients and with bloody diarrhea, sensitivity and specificity were 95.0%(19/20) and 86.4%(38/44), respectively, and overall agreement to culture method was 89.1%(57/64). One patient was found positive by culture method while negative in the present method, where the sample contained a low number of the cells less than the detection limit. Four of the six patients with a negative result by culture method and positive in the present method, were confirmed E. coli O157 infection by positive IgM antibody response against the E. coli O157 LPS. The discrepancy between the two methods seemed to be attributable to antibiotic adminis adminis-tration. In one patient, Salmonella urbana (O30₁30₂) was detected. The O30₁ antigen of this bacterium is well known to be identical to the E. coli O157 antigen.
  When the present method was compared with an ELISA-based E. coli O157 LPS antigen detection kit, sensitivity and specificity were 100%(11/11) and 82.1%(23/28), and overall agreement to ELISA method was 87.2%(34/39).
  From these findings, Quix^TM is useful as a rapid diagnostic kit in the primary clinics, outpatient or bedside use. E. coli O157 LPS antigen in patient's fecal samples can be detected in about five minutes with this simple procedure. Early diagnosis using such a simple kit will largely contribute for the early treatment and prevention of severe complications of the E. coli O157 infection.

  The Japanese Association for Infectious Diseases, 1998, Kansenshogaku Zasshi, 72(8) (8), 834 - 839, Japanese, Domestic magazine

  [Refereed]

  Scientific journal


• In Vitro Specific Binding of Shiga Toxin 1 and 2 by TAK-751S (Gb3 analog)

  TAKEDA Tae, YOSHINO Ken-ichi, ADACHI Eriko, YAMAGATA Kyohko, UCHIDA Hiroshi, OKONOGI Kenji, IIZAWA Yuji

  TAK-751S is a synthetic trisaccharide coupled to Chromosorb P using a spacer sequence of 8-methoxycarboyloctyl (MCO). Its chemical structure is similar to a human receptor (Gb3) of Stx produced by enterohemorrhagic Escherichia coli (EHEC). In vitro efficacy of TAK-715S was studied by using ACHN cultured cell assay, which is sensitive and specific for measuring low level of Stx.
  Under various conditions, TAK-751S was mixed with purified Stx1 and Stx2, and residual free toxins in the solution were measured by using ACHN cells. TAK-715S was demonstrated to bind specifically to Stx1 and Stx2 under the condition similar to a human intestine while Chromsorb P did not bind to any Stx. The binding activity was stable in the presence of various processed foods, fresh vegetables and fruits. Antibiotics such as fosfomycin, kanamycin and norfloxacin did not disturb its binding capability. Minimum inhibitory concentrations of these antibiotics against Staphylococcus aureus FDA209P or E. coli NIH J JC-2 neither changed after incubating with TAK-751S for 60 min at 37°C.
  These results suggest that TAK-751S can be given orally with various foods and antibiotics for the elimination of Stx1 and Stx2 in the gut of patients with EHEC infections.

  The Japanese Association for Infectious Diseases, 1998, Kansenshogaku Zasshi, 72(9) (9), 924 - 934, Japanese

  [Refereed]

  Scientific journal


• The novel heat-stable enterotoxin subtype gene (ystB) of Yersinia enterocolitica: Nucleotide sequence and distribution of the yst genes

  T. Ramamurthy, Ken-ichi Yoshino, Xiaozhe Huang, G. Balakrish Nair, Elisabeth Carniel, Tsutomu Maruyama, Hiroshi Fukushima, Tae Takeda

  Oct. 1997, Microbial Pathogenesis, 23(4) (4), 189 - 200, English

  [Refereed]

  Scientific journal


• A First Case of Hemolytic Uremic Syndrome Associated with Verotoxin-producing Escherichia coli O121:H19 in Japan

  SUZUKI Yoshiharu, OHNISHI Masako, SASAKI Hidekuni, YOSHINO Ken-ichi, TAKEDA Tae

  Sep. 1997, 日本小児科学会雑誌, 101(9) (9), 1403 - 1407, Japanese, Domestic magazine

  [Refereed]

  Scientific journal


• Purification, characterization and cloning of a novel variant of the superantigen Yersinia pseudoturberculosis-derived mitogen

  T. Ramamurthy, Ken-ichi Yoshino, Jun Abe, Norikazu Ikeda, Tae Takeda

  Aug. 1997, FEBS Letters, 413(1) (1), 174 - 176, English

  [Refereed]

  Scientific journal


• Nucleotide sequence of a gene encoding the novel Yersinia enterocolitica heat-stable enterotoxin that includes a pro-region-like sequence in its mature toxin molecule

  Xiaozhe Huang, Ken-ichi Yoshino, Hiroshi Nakao, Tae Takeda

  Feb. 1997, Microbial Pathogenesis, 22(2) (2), 89 - 97, English

  [Refereed]

  Scientific journal


• Identification of the functional region on the superantigen Yersinia pseudotuberculosis-derived mitogen responsible for induction of lymphocyte proliferation by using synthetic peptides

  Ken ichi Yoshino, Toshifumi Takao, Makiko Ishibashi, Yuji Samejima, Yasutsugu Shimonishi, Tae Takeda

  Lead, Jul. 1996, FEBS Letters, 390(2) (2), 196 - 198, English, International magazine

  [Refereed]

  Scientific journal


• Sensitive analysis of oligosaccharides derivatized with 4-aminobenzoic acid 2-(diethylamino)ethyl ester by matrix-assisted laser desorption/ ionization mass spectrometry

  Toshifumi Takao, Yanet Tambara, Akihiro Nakamura, Ken Ichi Yoshino, Hiroyuki Fukuda, Masafumi Fukuda, Yasutsugu Shimonishi

  1996, Rapid Communications in Mass Spectrometry, 10(6) (6), 637 - 640

  Scientific journal


• Characterization of a highly toxic, large molecular size heat-stable enterotoxin produced by a clinical isolate of Yersinia enterocolitica

  Ken-ichi Yoshino, Toshifumi Takao, Xiaozhe Huang, Hiroshi Murata, Hiroshi Nakao, Tae Takeda, Yasutsugu Shimonishi

  Lead, Apr. 1995, FEBS Letters, 362(3) (3), 319 - 322, English

  [Refereed]

  Scientific journal


• Geographical heterogeneity between Far East and Europe in prevalence of ypm gene encoding the novel superantigen among Yersinia pseudotuberculosis strains

  Ken-ichi Yoshino, Thandavarayan Ramamurthy, G. Balakrish Nair, Hiroshi Fukushima, Yoshimitsu Ohtomo, Nobuaki Takeda, Seiji Kaneko, Tae Takeda

  Lead, 1995, Journal of Clinical Microbiology, 33(12) (12), 3356 - 3358, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Purification and gene cloning of a superantigen (YPM) produced by a patient strain of Yersinia pseudotuberculosis.

  Tae TAKEDA, Ken-ichi YOSHINO, Yasuhiko. ITOH, Jun. ABE, Yasutsugu SHIMONISHI, Takao KOHSAKA

  1995, Contributions to Microbiology and Immunology, 13, 331 - 333, English

  [Refereed]

  International conference proceedings


• Sequence analysis of the gene for a novel superantigen produced by Yersinia pseudotuberculosis and expression of the recombinant protein

  Yasuhiko Ito, Jun Abe, Ken-ichi Yoshino, Tae Takeda, Takao Kohsaka

  1995, Journal of Immunology, 154(11) (11), 5896 - 5906, English

  [Refereed]

  Scientific journal


• Use of the Derivatizing Agent, 4-Aminobenzoic Acid 2-(Diethylamino)Ethyl Ester, for High-Sensitivity Detection of Oligosaccharides by Electrospray Ionization Mass Spectrometry

  Ken-ichi Yoshino, Toshifumi Takao, Hiroshi Murata, Yasutsugu Shimonishi

  Lead, 1995, Analytical Chemistry, 67(21) (21), 4028 - 4031, English

  [Refereed]

  Scientific journal


• Purification and characterization of a novel superantigen produced by a clinical isolate of Yersinia pseudotuberculosis

  Ken-ichi Yoshino, Jun Abe, Hiroshi Murata, Toshifumi Takao, Takao Kohsaka, Yasutsugu Shimonishi, Tae Takeda

  Lead, Dec. 1994, FEBS Letters, 356(1) (1), 141 - 144, English

  [Refereed]

  Scientific journal


• Amino acid sequence of a novel heat-stable enterotoxin produced by a yst gene-negative strain of Yersinia enterocolitica

  Ken-ichi Yoshino, Huang Xiaozhe, Miki Miyachi, Yeong Man Hong, Toshifumi Takao, Hiroshi Nakao, Tae Takeda, Yasutsugu Shimonishi

  Lead, Oct. 1994, Letters in Peptide Science, 1(2) (2), 95 - 105, English

  [Refereed]

  Scientific journal


• Identification and Characterization of Putative Receptors for Sperm‐Activating Peptide I (SAP‐I) in Spermatozoa of the Sea Urchin Hemicentrotus pulcherrimus

  Takeshi Shimizu, Ken‐ichi Yoshino, Norio Suzuki

  Apr. 1994, Development Growth & Differentiation, 36(2) (2), 209 - 221, English

  [Refereed]

  Scientific journal


• Purification and sequence determination of heat-stable enterotoxin elaborated by a cholera toxin-producing strain of Vibrio cholerae O1

  Ken-ichi Yoshino, Miki Miyachi, Toshifumi Takao, Prasanta K. Bag, Huang Xiaozhe, G. Balakrish Nair, Tae Takeda, Yasutsugu Shimonishi

  Lead, Jul. 1993, FEBS Letters, 326(1-3) (1-3), 83 - 86, English

  [Refereed]

  Scientific journal


• Sperm-activating peptide type-V (SAP-V), a fifth member of the sperm-activating peptide family, purified from the egg-conditioned media of the heart urchin Brissus agassizii

  Ken-ichi Yoshino, Toshifumi Takao, Yasutsugu Shimonishi, Norio Suzuki

  Lead, Elsevier BV, Aug. 1992, Comparative Biochemistry and Physiology Part B, 102(4) (4), 691 - 700, English

  [Refereed]

  Scientific journal


• Two classes of receptor specific for sperm‐activating peptide III in sand‐dollar spermatozoa

  Ken‐ichi Yoshino, Norio SUZUKI

  Lead, Jun. 1992, European Journal of Biochemistry, 206(3) (3), 887 - 893, English

  [Refereed]

  Scientific journal


• Determination of the amino acid sequence of an intramolecular disulfide linkage-containing sperm-activating peptide by tandem mass spectrometry

  Ken-ichi Yoshino, Toshifumi Takao, Yasutsugu Shimonishi, Norio Suzuki

  Lead, Dec. 1991, FEBS Letters, 294(3) (3), 179 - 182, English

  [Refereed]

  Scientific journal


• Identification of a Novel Amino Acid o-Bromo-L-phenylalanine in Egg-Associated Peptides That Activate Spermatozoa

  Ken-ichi Yoshino, Masaaki Yamaguchi, Norio Suzuki, Toshifumi Takao, Hideaki Hori, Yasutsugu Shimonishi, Masahiko Suhara, Tetsuya Kitai, Kohji Nomura

  Lead, Jun. 1991, Biochemistry, 30(25) (25), 6203 - 6209, English

  [Refereed]

  Scientific journal


• Analysis of post‐translational modifications of proteins by accurate mass measurement in fast atom bombardment mass spectrometry

  Toshifumi Takao, Ken‐ichi Yoshino, Norio Suzuki, Yasutsugu Shimonishi

  Nov. 1990, Biological Mass Spectrometry, 19(11) (11), 705 - 712, English

  [Refereed]

  Scientific journal


• A species-specific sperm-activating peptide from the egg jelly of the sea urchin Diadema setosum

  Ken-ichi Yoshino, Masanori Kurita, Masaaki Yamaguchi, Kohji Nomura, Toshifumi Takao, Yasutsugu Shimonishi, Norio Suzuki

  Lead, 1990, Comparative Biochemistry and Physiology, 95B(2) (2), 423 - 429, English

  [Refereed]

  Scientific journal


• Derivatives of sperm-activating peptide III (SAP-III) associated with eggs of sand dollars, Clypeaster japonicus and Astriclypeus manni

  Ken-ichi Yoshino, Toshifumi Takao, Masaaki Yamaguchi, Yasutsugu Shimonishi, Norio Suzuki

  Lead, 1990, Comparative Biochemistry and Physiology, 97B(4) (4), 679 - 685, English

  [Refereed]

  Scientific journal


• A halogenated amino acid-containing sperm activating peptide and its related peptides isolated from the egg jelly of sea urchins, Tripneustes gratilla, Pseudoboletia maculata, Strongylocentrotus nudus, Echinometra mathaei and Heterocentrotus mammillatus

  Ken-ichi Yoshino, Hiroko Kajiura, Kohji Nomura, Toshifumi Takao, Yasutsugu Shimonishi, Masanori Kurita, Masaaki Yamaguchi, Norio Suzuki

  Lead, 1989, Comparative Biochemistry and Physiology, 94B(4) (4), 739 - 751, English

  [Refereed]

  Scientific journal


• Some more speract derivatives associated with eggs of sea urchins Pseudocentrotus depressus, Strongylocentrotus purpuratus, Hemicentrotus pulcherrimus and Anthocidaris crassispina

  Norio Suzuki, Hiroko Kajiura, Kohji Nomura, David L. Garbers, Ken-ichi Yoshino, Masanori Kurita, Hiroshi Tanaka, Masaaki Yamaguchi

  1988, Comparative Biochemistry and Physiology, 89B(4) (4), 687 - 693, English

  [Refereed]

  Scientific journal


• A novel group of sperm activating peptides from the sea urchin Glyptocidaris crenularis

  Norio Suzuki, Ken-ichi Yoshino, Masanori Kurita, Kohji Nomura, Masaaki Yamaguchi

  1988, Comparative Biochemistry and Physiology, 90C(2) (2), 305 - 311, English

  [Refereed]

  Scientific journal


• Purification and structure of mosact and its derivatives from the egg jelly of the sea urchin Clypeaster japonicus

  Norio Suzuki, Masanori Kurita, Ken-ichi Yoshino, Hiroko Kajiura, Koji Nomura, Masaaki Yamaguchi

  Zoological Society of Japan, Aug. 1987, Zoological Science, 4(4) (4), 649 - 656, English, International magazine

  [Refereed]

  Scientific journal


• Speract binds exclusively to sperm tails and causes an electrophoretic mobility shift in a major sperm tail protein of sea urchins

  Norio Suzuki, Masanori Kurita, Ken-ichi Yoshino, Masaaki Yamaguchi

  Zoological Society of Japan, Aug. 1987, Zoological Science, 4(4) (4), 641 - 648, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

• 試験に出た質量分析（12）［変動範囲による原子量と同位体存在度の表記について］

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Jun. 2025, Journal of the Mass Spectrometry Society of Japan, 73(2) (2), 102 - 105, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析（11）［The 38th International Chemistry Olympiad, Korea, 2006］

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Jun. 2025, Journal of the Mass Spectrometry Society of Japan, 73(2) (2), 98 - 101, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析（10）［福井大学医学部入学試験問題（平成26年度）］

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Mar. 2025, Journal of the Mass Spectrometry Society of Japan, 73(1) (1), 45 - 48, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析（9）［大学入学共通テスト試験問題（令和6年度）］（後編）

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Dec. 2024, Journal of the Mass Spectrometry Society of Japan, 72(4) (4), 81 - 84, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析（8）［大学入学共通テスト試験問題（令和6年度）］（前編）

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Sep. 2024, Journal of the Mass Spectrometry Society of Japan, 72(3) (3), 46 - 49, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 第26回日本質量分析学会中部談話会開催報告

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Jun. 2024, Journal of the Mass Spectrometry Society of Japan, 72(2) (2), 35 - 38, Japanese, International magazine

  [Refereed]

  Meeting report


• 試験に出た質量分析（7）聖マリアンナ医科大学入学試験問題（令和2年度）

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Jun. 2024, Journal of the Mass Spectrometry Society of Japan, 72(2) (2), 27 - 30, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析 （6） 東京医科歯科大学入試問題（平成30年度）

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Mar. 2024, Journal of the Mass Spectrometry Society of Japan, 72(1) (1), 7 - 10, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 質量分析関係用語集2024追補版

  川崎ナナ, 絹見朋也, 佐藤貴弥, 角野浩史, 竹内孝江, 内藤康秀, 平田岳史, 福山繭子, 丸岡照幸, 南雅代, 本山晃, 吉野健一

  Last, 一般社団法人日本質量分析学会, 01 Mar. 2024, Journal of the Mass Spectrometry Socitey of Japan, 72(1) (1), 4 - 6, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• 第49回BMSコンファレンス開催報告

  吉野健一, 馬場健史

  Lead, 一般社団法人日本質量分析学会, 01 Dec. 2023, Journal of the Mass Spectrometry Society of Japan, 71(4) (4), 172 - 174, Japanese, Domestic magazine

  [Refereed]

  Meeting report


• 日本質量分析学会 部会・談話会の歴史

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Sep. 2023, Journal of the Mass Spectrometry Society of Japan, 71(3) (3), 103 - 106, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析（5）～第104回薬剤師国家試験問題（平成31年）～

  吉野健一

  Lead, 一般社団法人日本質量分析学会, 01 Sep. 2023, Journal of the Mass Spectrometry Society of Japan, 71(3) (3), 128 - 131, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• 試験に出た質量分析（4）～第65回臨床検査技師国家試験問題（平成31年）～

  吉野健一

  Lead, 01 Mar. 2023, 質量分析, 71(1) (1), 11 - 14, Japanese

  [Refereed]

  Introduction scientific journal


• Standard Definitions of Terms Related to Mass Spectrometry (Additional Edition 2023)

  Satoka Aoyagi, Nana Kawasaki, Tomoya Kinumi, Takaya Sato, Toshiki Sugai, Hirochika Sumino, Takae Takeuchi, Yasuhide Naito, Takafumi Hirata, Mayuko Fukuyama, Teruyuki Maruoka, Masayo Minami, Akira Motoyama, Ken-ichi Yoshino

  Last, The Mass Spectrometry Society of Japan, 01 Mar. 2023, Journal of the Mass Spectrometry Society of Japan, 71(1) (1), 15 - 18, Japanese

  [Refereed]

  Introduction scientific journal


• Online MS Colloquium by Kanto, Hokkaido, Tohoku and Chubu-Area Groups, and Diversity and Inclusion Committee: Lecture on Quantitative Analysis, Lab Tour, and Social Meeting

  Miwako Asanuma, Nana Kawasaki, Yuki Kobayashi, Kazumi Saikusa, Ryuichi Sawa, Kazutaka Shimbo, Kana Tanabe, Kohei Nozawa, Akira Harazono, Akira Motoyama, Hiroaki Akutsu, Seiko Oka, Shigeki Jin, Yusuke Takata, Ken-ichi Bajo, Tomohiro Hirose, Ayako Mori, Daisuke Saigusa, Emiko Sato, Tomoyoshi Soga, Akiyoshi Hirayama, Masamitsu Maekawa, Nariyasu Mano, Ken-ichi Yoshino, Issey Osaka, Sadamu Kurono, Keiko Kuwata, Tatsuko Sakai, Mitsutoshi Setou, Yutaka Takahashi, Yasuhide Naito, Tomohiro Matsumoto, Ken-ichi Yoshino, Keiko Kuwata

  The Mass Spectrometry Society of Japan, 01 Mar. 2022, Journal of the Mass Spectrometry Society of Japan, 70(1) (1), 85 - 89, Japanese

  Meeting report


• Standard Definition of Terms Relating to Mass Spectrometry (Additional Edition 2022)

  Aoyagi Satoka, Kawasaki Nana, Kinumi Tomoya, Sato Takaya, Sugai Toshiki, Takeuchi Takae, Naito Yasuhide, Hirata Takafumi, Fukuyama Mayuko, Maruoka Teruyuki, Motoyama Akira, Yoshino Ken-ichi

  Last, The Mass Spectrometry Society of Japan, 01 Mar. 2022, Journal of the Mass Spectrometry Society of Japan, 70(1) (1), 62 - 69, Japanese

  [Refereed]

  Introduction scientific journal


• 中田先生からの宿題

  吉野 健一

  Lead, 一般社団法人日本質量分析学会, 01 Oct. 2021, 質量分析, 69(5) (5), 98 - 99, Japanese, Domestic magazine

  [Invited]


• 中田尚男先生講演資料集

  黒野 定, 片木 宗弘, 堀山 志朱代, 吉野 健一

  Last, 一般社団法人日本質量分析学会, 01 Oct. 2021, 質量分析, 69(5) (5), 114 - 137, Japanese, Domestic magazine

  Others


• Selected Reaction Monitoring (SRM), Multiple Reaction Monitoring (MRM), and Consecutive Reaction Monitoring (CRM)

  Ken-ichi Yoshino

  Lead, The Mass Spectrometry Society of Japan, 01 Apr. 2021, Journal of the Mass Spectrometry Society of Japan, 69(2) (2), 30 - 33, Japanese

  [Refereed]

  Introduction scientific journal


• Mass Spectrometry in Exam Questions (3) ～The 102nd National Pharmacist Licensure Examination (2017) ～

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, 01 Jun. 2018, Journal of the Mass Spectrometry Society of Japan, 66(3) (3), 142 - 147, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• Mass Spectrometry in Exam Questions(2)～The 100th National Pharmacist Licensure Examination(2015)～

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, Apr. 2018, Journal of the Mass Spectrometry Society of Japan, 66(2) (2), 107 - 115, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• Mass Spectrometry in Exam Questions (1) ～The 98th National Pharmacist Licensure Examination (2013)～

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, Feb. 2018, Journal of the Mass Spectrometry Society of Japan, 66(1) (1), 43 - 48, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• Tamavidin-REVを用いたタンパク質間相互作用の網羅的解析

  Ueda Shuji, Yoshino Kenichi, 山田聡一郎

  Jan. 2018, 和光純薬時報, 86(1) (1), 6 - 7, Japanese

  [Invited]

  Introduction other


• Appropriate Usage of Analytical Instruments : Mass Spectrometry

  YOSHINO KEN-ICHI

  Lead, The Japanese Society for Analytical Chemistry, Jun. 2017, Benseki, 2017(6) (6), 216 - 223, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction scientific journal


• Standard Definitions of Terms Relating to Mass Spectrometry (additional edition)

  YOSHINO Ken-ichi, NAITO Yasuhide, KUBOTA Masayuki, KAWASAKI Hideya, IWAMOTO Kenichi, KINUMI Tomoya

  The Mass Spectrometry Society of Japan, 2017, Journal of the Mass Spectrometry Society of Japan, 65(2) (2), 76 - 90, Japanese

  [Refereed]

  Introduction scientific journal


• PKCγKOパーキンソン症候群モデル βPIXリン酸化のドパミン遊離での役割

  白藤 俊彦, 上山 健彦, 吉野 健一, 足立 直子, 高橋 英之, 平松 直樹, 吾郷 由希夫, 松田 敏夫, 戸田 達史, 酒井 規雄, 齋藤 尚亮

  (一社)日本神経学会, Dec. 2014, 臨床神経学, 54(Suppl.) (Suppl.), S242 - S242, Japanese


• Abbreviations of Combined Methods and Combined Instruments

  YOSHINO Ken-ichi

  IUPAC (International Union of Pure and Applied Chemistry) recommends that a hyphen, or, alternatively, a slash, should be used to indicate a combination of two analytical techniques such as liquid chromatography separation combined with mass spectrometry detection, in the last definitions of terms relating to mass spectrometry. Thus, such combined methods can be written as liquid chromatography-mass spectrometry or alternatively liquid chromatography/mass spectrometry. The corresponding abbreviations are LC-MS or LC/MS.

  The Mass Spectrometry Society of Japan, 01 Oct. 2014, Journal of the Mass Spectrometry Society of Japan, 62(5) (5), 61 - 64, Japanese

  [Refereed]

  Introduction scientific journal


• A protein kinase C-gamma knockout Parkinsonian model: Involvement of Pak-Interacting Exchange Factor-beta

  Toshihiko Shirafuji, Takehiko Ueyama, Ken-ichi Yoshino, Naoko Adachi, Hideyuki Takahashi, Naoki Hiramatsu, Yukio Ago, Toshio Matsuda, Norio Sakai, Naoaki Saito

  2014, JOURNAL OF PHARMACOLOGICAL SCIENCES, 124, 207P - 207P, English

  Summary international conference


• Usage of the Term MS/MS Spectrum

  YOSHINO Ken-ichi

  The "Definitions of terms relating to mass spectrometry [IUPAC (International Union of Pure and Applied Chemistry) recommendations 2013]" define the term "MS/MS spectrum" as "a mass spectrum obtained using tandem mass spectrometry." The semantic content of the term "MS/MS spectrum" is not only a product ion mass spectrum but also a precursor ion spectrum and a neutral loss spectrum. However, the term "MS/MS spectrum" is frequently used as a synonym of a product ion spectrum. An "MS/MS spectrum of the peptide angiotensin I" does not convey the same meaning as the "product ion spectrum of the peptide angiotensin I."

  The Mass Spectrometry Society of Japan, 2014, Journal of the Mass Spectrometry Society of Japan, 62(6) (6), 115 - 118, Japanese

  [Refereed]

  Introduction scientific journal


• Report of the 40th Biological Mass Spectrometry Conference

  TAKEUCHI Atsuko, YOSHINO Ken-ichi

  Last, The Mass Spectrometry Society of Japan, Oct. 2013, Journal of the Mass Spectrometry Society of Japan, 61(5) (5), 47 - 52, Japanese, Domestic magazine

  [Refereed]

  Meeting report


• 蛍光イメージングを用いた神経細胞でのシャペロン介在性オートファジー活性の解析

  関 貴弘, 吉野 健一, 田中 茂, 土肥 栄祐, 隠地 智也, 秀 和泉, Paulson Henry, 齋藤 尚亮, 酒井 規雄

  日本臨床精神神経薬理学会・日本神経精神薬理学会, Oct. 2013, 日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集, 23回・43回, 249 - 249, Japanese


• 意外に知らない分子量と質量の単位の違い

  Yoshino Kenichi

  Aug. 2013, 生物工学会誌, 91(8) (8), 464 - 468, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• Analysis of PKCY substrates in nigro-striatum system:The role of beta PIX phosphorylation in the dopamine release

  Toshihiko Shirafuji, Takehiko Ueyama, Kenichi Yoshino, Naoko Adachi, Hideyuki Takahashi, Yukio Ago, Toshio Matsuda, Naoaki Saito

  2013, JOURNAL OF PHARMACOLOGICAL SCIENCES, 121, 122P - 122P, English

  Summary international conference


• 黒質線条体系におけるPKCγの基質の解析

  白藤 俊彦, 上山 健彦, 吉野 健一, 足立 直子, 高橋 英之, 香田 健, 梨子田 哲明, 吾郷 由希夫, 松田 敏夫, 齋藤 尚亮

  (公社)日本薬理学会, Oct. 2012, 日本薬理学雑誌, 140(4) (4), 1P - 1P, Japanese


• 無細胞再構成系によるヌクレオチド除去修復活性制御因子の探索

  戸根大輔, 吉野健一, 岩井成憲, 菅澤薫

  2012, 日本分子生物学会年会プログラム・要旨集(Web), 35th


• Does mutant gamma PKC causing spinocerebellar ataxia type 14 (SCA14) affect chaperone-mediated autophagy (CMA)? -Attempt to measure CMA activity using a novel indicator-

  Takahiro Seki, Ken-ichi Yoshino, Tomoya Onji, Shigeru Tanaka, Izumi Hide, Naoaki Saito, Norio Sakai

  2011, JOURNAL OF PHARMACOLOGICAL SCIENCES, 115, 247P - 247P, English

  Summary international conference


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～最終回

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 山岡 寛史

  化学同人, Dec. 2010, 化学, 65(12), 62-65(12) (12), 62 - 65, Japanese

  [Refereed]

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～⑩

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 山岡 寛史

  化学同人, Nov. 2010, 化学, 65(11), 28-30(11) (11), 28 - 30, Japanese

  [Refereed]

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～⑨

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 山岡 寛史

  化学同人, Sep. 2010, 化学, 65(9), 41-44(1) (1), 59 - 61, Japanese

  [Refereed]

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～⑧

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 山岡 寛史

  化学同人, Aug. 2010, 化学, 65(8) (8), 56 - 59, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～⑦

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 中林 安雄

  化学同人, Jul. 2010, 化学, 65(7), 56-58(7) (7), 56 - 58, Japanese

  [Refereed]

  Introduction scientific journal


• Detectors for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer

  TANAKA Koichi, YOSHINO Ken-ichi, HAYASHI Masahiro, NAITO Yasuhide, OHKUBO Masataka

  In general, microchannel plate (MCP) is used as the detector for time-of-flight mass spectrometer (TOF-MS). In addition to the MCP, TOF-MS equipped with modified secondary electron multiplier (SEM) is commercially available. A superconducting tunnel junction (STJ) detector, which enables measurement of kinetic energy in addition to time-of-flight, has been developed for TOF-MS. MCP and SEM detectors give only m/z values, which remains ambiguous over charge number (z). Since kinetic energy is proportional to z, TOF-MS equipped with STJ detector is possible to determine mass (m) unambiguously. In this commentary, the features of MCP and modified SEM will be described. Furthermore, we give an outline of the STJ detector.

  The Mass Spectrometry Society of Japan, 01 Jun. 2010, Journal of the Mass Spectrometry Society of Japan, 58(3) (3), 111 - 116, Japanese

  [Refereed]

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～⑥

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 山岡 寛史

  化学同人, Jun. 2010, 化学, 65(6), 44-47(6) (6), 44 - 47, Japanese

  [Refereed]

  Introduction scientific journal


• Phosphopeptide analysis by LC/MS[n] with a linear ion trap mass spectrometer

  Ken-ichi Yoshino

  科学評論社, Apr. 2010, Endocrinology, diabetology & metabolism, 30(4) (4), 377 - 385, Japanese

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～⑤

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 中林 安雄

  化学同人, Apr. 2010, 化学, 65(4), 54-57(4) (4), 54 - 57, Japanese

  [Refereed]

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～④

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 中林 安雄

  化学同人, Mar. 2010, 化学, 65(3), 45-47(3) (3), 45 - 47, Japanese

  [Refereed]

  Introduction scientific journal


• Comments on the Redundant Expressions "MS-Bunseki," : "MS-Kaiseki" and "MS-Sokutei"

  YOSHINO Ken-ichi

  “MS” is an abbreviation for “mass spectrometry.” “Bunseki” and “Kaiseki” in Japanese are equivalent for “analysis” in English. A Japanese word “Sokutei” is for the English word “measurement.” Thus, the Japanese words “MS-Bunseki,” “MS-Kaiseki” and “MS-Sokutei” are redundant expressions.

  Lead, The Mass Spectrometry Society of Japan, 01 Feb. 2010, Journal of the Mass Spectrometry Society of Japan, 58(1) (1), 29 - 34, Japanese

  Introduction scientific journal


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～③

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也, 中林 安雄

  化学同人, Feb. 2010, 化学, 65(2), 46-48(2) (2), 46 - 48, Japanese

  [Refereed]

  Introduction scientific journal


• Novel phosphorylation site(s) of cofilin by PKC alpha and its role in the degranulation from RBL-2H3 mast cells

  Megumi Sakuma, Yasuhito Shirai, Ken-ichi Yoshino, Maho Kuramasu, Tomofumi Nakamura, Izumi Hide, Yoshihiro Nakata, Naoaki Saito

  2010, JOURNAL OF PHARMACOLOGICAL SCIENCES, 112, 54P - 54P, English

  Summary international conference


• Mutant gamma PKC causing spinocerebellar ataxia type 14 (SCA14) preferably interacts with heat shock cognate protein 70 (Hsc70) and affects its translocation to lysosome

  Takahiro Seki, Ken-ichi Yoshino, Kouta Ogawa, Tomoya Onji, Shigeru Tanaka, Izumi Hide, Naoaki Saito, Norio Sakai

  2010, NEUROSCIENCE RESEARCH, 68, E196 - E196, English

  Summary international conference


• クイズで学ぶ用語の誤用～化学者なら専門用語も正確に～②

  Ken-ichi Yoshino, 阿竹 徹, 川瀬 雅也

  化学同人, Jan. 2010, 化学, 65(1), 59-61(1) (1), 59 - 61, Japanese

  [Refereed]

  Introduction scientific journal


• Definitions of Incorrect and Unofficial Terms Relating to Mass Spectrometry

  YOSHINO Ken-ichi

  This is a mass spectrometry glossary recording of daily used “unofficial terms” which are not recorded in the official glossary. In addition, the misuse examples which ignore the definition of scientific terminology and the terms which become the obsolete words officially are recorded.

  Lead, The Mass Spectrometry Society of Japan, 01 Dec. 2009, Journal of the Mass Spectrometry Society of Japan, 57(6) (6), 379 - 385, Japanese

  [Refereed]

  Introduction scientific journal


• 間違えやすい質量分析基礎用語

  Ken-ichi Yoshino

  公益社団法人 日本農芸化学会, 01 Jun. 2009, 化学と生物, 47(6) (6), 436 - 440, Japanese

  Introduction scientific journal


• マススペクトルの横軸ラベルは"m/z"に限ります ～御用だ誤用だ！ 「用語」の誤用、第12回

  Yoshino Ken-ichi

  Apr. 2009, 化学, 64(4) 43, Japanese

  Introduction scientific journal


• “TIC”はtotal ion chromatogramの略語にあらず！～御用だ誤用だ！ 「用語」の誤用、第11回

  Yoshino Ken-ichi

  Mar. 2009, 化学, 64(3), 43, Japanese

  Introduction scientific journal


• The Deprecated Term "Daughter Ion"

  YOSHINO Ken-ichi

  Lead, The Mass Spectrometry Society of Japan, 01 Feb. 2009, Journal of the Mass Spectrometry Society of Japan, 57(1) (1), 37 - 39, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• The Deprecated Term "Parent Ion"

  YOSHINO Ken-ichi

  Lead, The Mass Spectrometry Society of Japan, 01 Feb. 2009, Journal of the Mass Spectrometry Society of Japan, 57(1) (1), 40 - 41, Japanese

  [Refereed]

  Introduction scientific journal


• Principles of the Delayed Extraction Technique

  TANAKA Koichi, SATOH Takaya, YOSHINO Ken-ichi

  The delayed extraction (also known as pulsed extraction) technique involves the application of a time delay between the ionization pulse and an accelerating voltage pulse for desorption/ionization from a surface. This technique can achieve energy focusing in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer and is used to improve mass resolving power. We explain the principles of delayed extraction (pulsed extraction) used in a MALDI TOF mass spectrometer.

  The Mass Spectrometry Society of Japan, 01 Feb. 2009, Journal of the Mass Spectrometry Society of Japan, 57(1) (1), 31 - 36, Japanese

  [Refereed]

  Introduction scientific journal


• トリプシンは制限酵素にあらず！ ～御用だ誤用だ！ 「用語」の誤用、第10回

  Yoshino Ken-ichi

  Feb. 2009, 化学, 64(2), 23, Japanese

  Introduction scientific journal


• Cofilin is phosphorylated by cPKC in the degranulation from RBL-2H3 mast cells

  Megumi Sakuma, Yasuhito Shirai, Maho Kuramasu, Kenichi Yoshino, Tomofumi Nakamura, Izumi Hide, Yoshihiro Nakata, Naoaki Saito

  2009, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 236P - 236P, English

  Summary international conference


• “Mass Spectrometer”は質量分析器にあらず ～御用だ誤用だ！ 「用語」の誤用、第９回

  Yoshino Ken-ichi

  Jan. 2009, 化学, 64(1), 61, Japanese

  Introduction scientific journal


• Why Can Reflectron Mode in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Perform MS/MS-like Analysis?

  TANAKA Koichi, YOSHINO Ken-ichi, KINUMI Tomoya, TAKAYAMA Mitsuo

  By measurement of post-source decay (PSD) product ion mass spectrum, matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOFMS) enable mass spectrometry/mass spectrometry (MS/MS)-like analysis to obtain structural information. We will explain the basics of MS/MS-like analysis in reflectron mode of MALDI TOFMS by comparing post-source decay product ion mass spectrum acquired by the standard reflectron and the curved field reflectron.

  The Mass Spectrometry Society of Japan, 01 Dec. 2008, Journal of the Mass Spectrometry Society of Japan, 56(6) (6), 263 - 268, Japanese

  [Refereed]

  Introduction scientific journal


• Units of Atomic Mass ; "u," "Da," "amu," and "mmu"

  YOSHINO Ken-ichi

  The unified atomic mass unit (unit symbol: u) is a non-SI unit of mass, defined as one-twelfth the mass of a single ¹²C atom in its ground state. [SI: Le Système International d'Unités; The International System of Units] This definition was agreed upon by both the International Union of Pure and Applied Physics (IUPAP) and the International Union of Pure and Applied Chemistry (IUPAC) in the early 1960s to resolve a longstanding difference between two scales of the atomic mass unit. The term “atomic mass unit” (unit symbol: amu) has been used as a unit of mass defined as one-sixteenth the mass of a single atom ¹⁶O [m(¹⁶O)=16 amu] in physics and as one-sixteenth the isotope-averaged atomic mass (equivalent to the atomic weight) of oxygen [A_r(O)=16 amu] in chemistry.
  The unit dalton (unit symbol: Da) is also a non-SI unit of mass defined as “1 Da=1 u,” and is accepted as a unit for use by the SI in the 8th edition of the SI brochure (2006). Therefore, both the unified atomic mass unit and dalton are authorized units for mass of ions and molecules.
  It is a common mistake to use the deprecated term “atomic mass unit” and the deprecated unit symbol “amu” for the unit of mass defined as one-twelfth the mass of single atom ¹²C. The unit symbol “mmu,” meaning a millimass unit, is also an appropriate unit in SI. Instead of “mmu,” “mDa” or and “10^-3 u” should be used.

  The Mass Spectrometry Society of Japan, 01 Dec. 2008, Journal of the Mass Spectrometry Society of Japan, 56(6) (6), 269 - 274, Japanese

  [Refereed]

  Introduction scientific journal


• kDaは分子量の単位ではありません ～御用だ誤用だ！ 「用語」の誤用、第８回

  Yoshino Ken-ichi

  Dec. 2008, 化学, 63(12), 41, Japanese

  Introduction scientific journal


• 控えましょう！ “mmu”を使うこと ～御用だ誤用だ！ 「用語」の誤用、第７回

  Yoshino Ken-ichi

  Nov. 2008, 化学, 63(11), 25, Japanese

  Introduction scientific journal


• Comments on Using of the Alias "Masu" for Units of Mass and m/z

  YOSHINO Ken-ichi

  The Japanese word “masu” originates from the English word “mass.” It is occasionally used as an alias for units of mass and m/z. However, mass of molecules and ions should be expressed with the unified atomic mass unit (unit symbol: u) or dalton (unit symbol: Da). Furthermore, the m/z value indicated in the abscissa of mass spectra is dimensionless; therefore, it should not have any units.

  Lead, The Mass Spectrometry Society of Japan, 01 Oct. 2008, Journal of the Mass Spectrometry Society of Japan, 56(5) (5), 241 - 245, Japanese, Domestic magazine

  Introduction scientific journal


• 「amu」は、半世紀前に廃止された単位です ～御用だ誤用だ！ 「用語」の誤用、第６回

  Yoshino Ken-ichi

  Sep. 2008, 化学, 63(9), 25, Japanese

  Introduction scientific journal


• 使い分けましょう！ ２種類の「精密質量」 ～御用だ誤用だ！ 「用語」の誤用、第５回

  Yoshino Ken-ichi

  Aug. 2008, 化学, 63(8), 17, Japanese

  Introduction scientific journal


• "m/z" and "Mass-to-Charge Ratio"

  YOSHINO Ken-ichi

  Mass spectrometers separate ions according to each mass/charge value, which is not a dimensionless quantity. However, the m/z value indicated in the abscissa of mass spectra is defined as a dimensionless quantity and not a numerical value the same as the mass/charge value in kilograms/coulombs (kg/C) or in unified atomic mass units/coulombs (u/C). The numerical value of m/z converted from the mass/charge value depends on the unified atomic mass unit and the physical constant “elementary charge,” e that is also the atomic unit of charge. This conversion confuses many mass spectrometrists with regard to the abscissa labeling of mass spectra, e.g. the symbol “m/z” is considered as an abbreviation of the term “mass-to-charge ratio.”

  Lead, The Mass Spectrometry Society of Japan, 01 Aug. 2008, Journal of the Mass Spectrometry Society of Japan, 56(4) (4), 209 - 213, Japanese

  [Refereed]

  Introduction scientific journal


• 細胞組織化学におけるプロテオミクス

  吉野 健一

  Lead, 日本組織細胞化学会, 10 Jul. 2008, 組織細胞化学2008ーイメージングテクニックの入門とその応用ー, 75 - 85, Japanese, Domestic magazine

  [Invited]

  Lecture materials


• 控えましょう？ 「娘イオン」を使うこと ～御用だ誤用だ！ 「用語」の誤用、第４回

  Yoshino Ken-ichi

  Jul. 2008, 化学, 63(7), 44, Japanese

  Introduction scientific journal


• "Mass Value" and "Mass Number"

  YOSHINO Ken-ichi

  Some of mass spectrometrists refer to a term “mass number” as physical quantities of mass value and m/z value. However, the term “mass number” is defined as the sum of the total number of protons and neutrons in an atomic nucleus, atom, molecule, ion, or radical.

  The Mass Spectrometry Society of Japan, 01 Jun. 2008, Journal of the Mass Spectrometry Society of Japan, 56(3) (3), 173 - 178, Japanese

  [Refereed]

  Introduction scientific journal


• これからかわります！ 「質量電荷比」の使い方 ～御用だ誤用だ！ 「用語」の誤用、第３回

  Yoshino Ken-ichi

  Jun. 2008, 化学, 63(6), 38, Japanese

  Introduction scientific journal


• まちがえていませんか？ 「分子イオン」の使い方 ～御用だ誤用だ！ 「用語」の誤用、第２回

  Yoshino Ken-ichi

  May 2008, 化学, 63(5), 40, Japanese

  Introduction scientific journal


• The Incorrect Term "Protonated Molecular Ion"

  YOSHINO Ken-ichi

  Lead, The Mass Spectrometry Society of Japan, 01 Apr. 2008, Journal of the Mass Spectrometry Society of Japan, 56(2) (2), 55 - 56, Japanese

  [Refereed]

  Introduction scientific journal


• Comments on Usage of the Alias “Mirimasu”

  Ken-ichi YOSHINO

  The Japanese word “mirimasu” originates from the English words “milli” and “mass.” It is normally used as an alias for “measured accurate mass” and “accurate mass measurement.” It causes confusions that “mirimasu” is also used as an alias for “high resolution mass spectrometry,” “high resolution mass spectrum (spectra),” and “mass spectrometer with high mass resolving power.” This nomenclature results from the fact that “high resolution” is confused with “high accuracy.” In addition to these interpretations, Japanese mass spectrometrists also use “mirimasu” as an alias for “one-thousandth of a unified atomic mass unit,” “one-thousandth of a dalton (mDa),” and “one-thousandth of an m/z unit.” This incorrect usage is due to a lack of understanding of the unit system of atomic mass and m/z on mass spectra. Therefore, “mirimasu” should not be used in Japanese, because it is ambiguous and involves various problems and misunderstandings.

  Lead, The Mass Spectrometry Society of Japan, 01 Apr. 2008, Journal of the Mass Spectrometry Society of Japan, 56(2) (2), 69 - 76, Japanese, Domestic magazine

  Introduction scientific journal


• Confusion between the Japanese Alias "Mirimasu" and the Mass Spectrometer with High Mass Resolving Power

  TAKINAMI Yoshihiko, YOSHINO Ken-ichi

  The Mass Spectrometry Society of Japan, 01 Apr. 2008, Journal of the Mass Spectrometry Society of Japan, 56(2) (2), 57 - 57, Japanese

  [Refereed]

  Introduction scientific journal


• まちがえていませんか？ 「質量数」の使い方 ～御用だ誤用だ！ 「用語」の誤用、第1回

  Yoshino Ken-ichi

  Apr. 2008, 化学, 63(4), 48, Japanese

  Introduction scientific journal


• Why Can Reflectron and Multi-Turn Time-of-Flight Mass Spectrometer Improve Their Mass Resolving Powers?

  TANAKA Koichi, YOSHINO Ken-ichi, NAITO Yasuhide, TOYODA Michisato

  Linear mode, reflectron mode, and multi-turn are techniques for m/z analysis in time-of-flight mass spectrometry. Reflectron mode and multi-turn enable analysis to be performed with a higher mass resolving power than that attained with linear mode. In this commentary, starting from the basic principles of time-of-flight mass spectrometry in linear mode, the possible improvements in mass resolving power that can be made by reflectron mode and multi-turn techniques will be explained.

  The Mass Spectrometry Society of Japan, 01 Apr. 2008, Journal of the Mass Spectrometry Society of Japan, 56(2) (2), 49 - 54, Japanese

  [Refereed]

  Introduction scientific journal


• The Misuse of the Term "Mass Number" When Used to Refer to Mass Value

  YOSHINO Ken-ichi

  Lead, The Mass Spectrometry Society of Japan, 01 Feb. 2008, Journal of the Mass Spectrometry Society of Japan, 56(1) (1), 21 - 22, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• Comments on the Usage of the Terms “Accurate Mass”and “Exact Mass”

  Ken-ichi YOSHINO

  The terms “accurate mass” and “exact mass” are not synonymous in mass spectrometry. Accurate mass refers to the mass value of an ion or radical determined experimentally by mass spectrometry. On the other hand, exact mass refers to the mass value of a single ion, radical, or molecule calculated by summing the mass values of individual isotopes. To avoid confusion, “measured accurate mass” and “calculated exact mass” should be used in place of “accurate mass” and “exact mass,” respectively.

  Lead, The Mass Spectrometry Society of Japan, 01 Feb. 2008, Journal of the Mass Spectrometry Society of Japan, 56(1) (1), 27 - 32, Japanese, Domestic magazine

  Introduction scientific journal


• RalAはηPKCによるケラチノサイトの扁平化に必須である

  白井康仁, 盛岡祥子, 大辻千裕, 吉野健一, 酒井規雄, 上山健彦, 齋藤尚亮

  2008, 日本薬理学雑誌, 132(3) (3)


• Comments on the Usage of the Abbreviation "TIC" for "Total Ion Chromatogram"

  Ken-ichi YOSHINO

  In terms related to mass spectrometry, “TIC” should be used as an abbreviation for “total ion current.” However, many mass spectrometrists use “TIC” as an abbreviation for “total ion chromatogram” while referring to “total ion current chromatogram,” which is incorrect. Another common misunderstanding related to abbreviations is the usage of “MCP” as an abbreviation for “multichannel plate,” which should be used for “microchannel plate.”

  Lead, The Mass Spectrometry Society of Japan, 01 Dec. 2007, Journal of the Mass Spectrometry Society of Japan, 55(6) (6), 381 - 388, Japanese, Domestic magazine

  Introduction scientific journal


• Comments on Usage of the Term "Mass Spectrograph"

  YOSHINO Ken-ichi

  Similar to a mass spectrometer, mass spectrograph is an instrument used to obtain mass spectra. In the mass spectrograph, ions with different m/z values are passed through a magnetic sector and dispersed in different trajectories similar to the dispersion of different wavelengths of light in a prism. The ions separated according to their m/z values are directed onto the same face and are simultaneously detected by a focal plane detector. This mass spectrum-measurement system is unique for instruments called as “mass spectrographs.” However, many mass spectrometrists refer to instruments that should be called “mass spectrometers” as “mass spectrographs.”

  Lead, The Mass Spectrometry Society of Japan, 01 Oct. 2007, Journal of the Mass Spectrometry Society of Japan, 55(5) (5), 353 - 361, Japanese, Domestic magazine

  Introduction scientific journal


• Benefits of Contributing to the JMSSJ

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, 01 Aug. 2007, Journal of the Mass Spectrometry Society of Japan, 55(4) (4), 294 - 297, Japanese, Domestic magazine

  Others


• Comments on Usage of the Abbreviation "MS" and the Japanese Abbreviation "Masu"

  Ken-ichi YOSHINO

  The term “mass spectrometry” is usually abbreviated as “MS.” It is confusing that “MS” is also used as an abbreviation for “mass spectrometer,” “mass spectroscopy,” “mass spectrograph,” “mass spectrum (spectra),” and “mass separation.” “Masu” in Japanese is an ambiguous word and originates from “mass” in English or “Mass” in German. In addition to the meaning of mass, however, “masu” is used as an abbreviation for “mass spectrometry,” “mass spectrometer,” “unified atomic mass unit,” and “a quantity of m/z= 1” by Japanese mass spectrometrists. Because of the ambiguity in the usage of “MS” and “masu,” some inappropriate terms, such as “peptide MS fingerprinting” for “peptide mass fingerprinting” and “TOF-mass” for “time-of-flight mass spectrometer,” were used by Japanese mass spectrometrists.

  Lead, The Mass Spectrometry Society of Japan, 01 Aug. 2007, Journal of the Mass Spectrometry Society of Japan, 55(4) (4), 298 - 309, Japanese, Domestic magazine

  Introduction scientific journal


• Comments on Usage of the Term "Restriction Enzyme"

  Ken-ichi YOSHINO

  The term “restriction enzyme” is derived from the phenomenon of host-controlled restriction and modification in viral infection of bacteria, not from the ability of enzymes to recognize the specific target nucleotide sequence in the DNA and break the DNA chain. That is, the term “restriction enzyme” is not derived from the fact that the cleavage site is restricted. Bacteria initially resist infections by new bacteriophages, and this “restriction” of viral growth stems from endonucleases within the cells that destroy foreign DNA molecules from bacteriophages. Restriction enzymes are a kind of nuclease that catalyze hydrolysis of DNA strands and were discovered during investigations of the phenomenon of host-controlled restriction and modification of bacteriophages. They are not peptidases (proteases). However, it is a common mistake to use the term “restriction enzyme” for amino acid residue-specific endopeptidase such as trypsin.

  Lead, The Mass Spectrometry Society of Japan, 01 Jun. 2007, Journal of the Mass Spectrometry Society of Japan, 55(3) (3), 245 - 260, Japanese, Domestic magazine

  Introduction scientific journal


• Comments on Usage of the Terms “Parent Ion” and “Daughter Ion”

  Ken-ichi YOSHINO

  Ions are not organisms, and thus they do not proliferate by either sexual or asexual reproduction. They are hence incapable of being parents, children, or grandchildren. Therefore, the terms “parent ion,” “daughter ion,” and “granddaughter ion” are scientifically incorrect. In addition, mass spectrometrists should note that the terms “daughter ion” and “granddaughter ion” are gender-specific. (The terms “daughter nuclide” and “granddaughter nuclide” are also gender-specific.)
  Therefore, the terms “parent ion” and “daughter ion” need to be replaced by “precursor ion” and “product ion,” respectively. Instead of the gender-specific terms “granddaughter ion” and “great-granddaughter ion,” the gender-neutral terms “second generation product ion” and “third generation product ion” should be used.

  Lead, The Mass Spectrometry Society of Japan, 01 Apr. 2007, Journal of the Mass Spectrometry Society of Japan, 55(2) (2), 99 - 115, Japanese, Domestic magazine

  Introduction scientific journal


• Comments on Abscissa Labeling of Mass Spectra

  Ken-ichi YOSHINO

  Mass spectrometers separate ions according to each mass/charge value, which is not a dimensionless quantity. However, the m/z value indicated in the abscissa of mass spectra is defined as a dimensionless quantity and not a numerical value the same as the mass/charge value in kilograms/coulombs (kg/C). The numerical value of m/z converted from the mass/charge value depends on the unified atomic mass unit, u (or dalton, Da) and the physical constant “elementary charge,” e that is also the atomic unit of charge. This conversion confuses many mass spectrometrists with regard to the abscissa labeling of mass spectra. To avoid this confusion, a new unit, “thomson” (unit symbol, Th), was proposed, and defined as 1 Th=1 u/atomic charge=1 Da/atomic charge. However, this unit is not yet well known and has been deprecated.

  Lead, The Mass Spectrometry Society of Japan, 01 Feb. 2007, Journal of the Mass Spectrometry Society of Japan, 55(1) (1), 51 - 62, Japanese, Domestic magazine

  Introduction scientific journal


• A Deprecated Term "Molecular-Related Ion"

  YOSHINO Ken-ichi

  Lead, The Mass Spectrometry Society of Japan, 01 Dec. 2006, Journal of the Mass Spectrometry Society of Japan, 54(6) (6), 255 - 255, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• The Difference between Unit Symbols "u", "Da", "amu", and "mmu"

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, 01 Dec. 2006, Journal of the Mass Spectrometry Society of Japan, 54(6) (6), 256 - 257, Japanese, International magazine

  [Refereed]

  Introduction scientific journal


• Comments on Usage of the Unit Symbol "amu"

  Ken-ichi YOSHINO

  The unified atomic mass unit (unit symbol: u) is a non-SI unit of mass defined as one twelfth the mass of a single ¹²C atom in its ground state. This definition was agreed upon by both the International Union of Pure and Applied Physics and the International Union of Pure and Applied Chemistry in early 1960s to resolve a longstanding difference between two scales of atomic mass unit. The term “atomic mass unit” (unit symbol: amu) has been used to a unit of mass defined as one sixteenth the mass of a single atom ¹⁶O [m(¹⁶O)=16 amu] in physics, or as one sixteenth the isotope-averaged atomic mass (equivalent to the atomic weight) of oxygen [A_r(O)=16 amu] in chemistry.
  It is a common mistake to use the deprecated term “atomic mass unit” and the deprecated unit symbol “amu” for the unit of mass defined as one twelfth the mass of single atom ¹²C.

  Corresponding, The Mass Spectrometry Society of Japan, 01 Dec. 2006, Journal of the Mass Spectrometry Society of Japan, 54(6) (6), 259 - 271, Japanese, International magazine

  Introduction scientific journal


• Comments on Usage of the Term "Mass-to-Charge Ratio"

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, 01 Oct. 2006, Journal of the Mass Spectrometry Society of Japan, 54(5) (5), 219 - 227, Japanese, International magazine

  Introduction scientific journal


• The Unit Symbol "Th"

  Yasuhide NAITO, Ken-ichi YOSHINO

  The Mass Spectrometry Society of Japan, 01 Oct. 2006, Journal of the Mass Spectrometry Society of Japan, 54(5) (5), 218 - 218, Japanese

  [Refereed]

  Introduction scientific journal


• Comments on Usage of the Symbol "m/z"

  Ken-ichi YOSHINO

  Lead, The Mass Spectrometry Society of Japan, 01 Aug. 2006, Journal of the Mass Spectrometry Society of Japan, 54(4) (4), 175 - 182, Japanese, International magazine

  Introduction scientific journal


• Comments on Usage of the Terms "Sodium Adduct Ion" and "Protonated Molecular Ion"

  Ken-ichi YOSHINO

  Lead, 日本質量分析学会, 01 Jun. 2006, Journal of the Mass Spectrometry Society of Japan, 54(3) (3), 119 - 126, Japanese, International magazine

  Introduction scientific journal


• Comments on Usage of the Term "Molecular Ion"

  Ken-ichi YOSHINO

  Lead, 日本質量分析学会, 01 Apr. 2006, Journal of the Mass Spectrometry Society of Japan, 54(2) (2), 57 - 63, Japanese

  Introduction scientific journal


• Mammalian target of rapamycin (mTOR)

  吉野 健一, 米澤 一仁

  Lead, 11 Feb. 2006, 医学のあゆみ, 216(6) (6), 477 - 478, Japanese

  [Invited]

  Introduction commerce magazine


• Comments on Usage of the Term "Mass Number"

  Ken-ichi YOSHINO

  Lead, 日本質量分析学会, 01 Feb. 2006, Journal of the Mass Spectrometry Society of Japan, 54(1) (1), 33 - 37, Japanese, International magazine

  Introduction scientific journal


• Good Medicine Is Bitter in the Mouth

  YOSHINO Ken-ichi

  Lead, 日本質量分析学会, 01 Dec. 2005, 質量分析, 53(6) (6), 335 - 336, Japanese, Domestic magazine

  Others


• The 53rd ASMS Conference (San Antonio,Texas) レポート FIESTA ASMS OLE!

  吉野 健一

  Lead, 日本質量分析学会, 01 Oct. 2005, 質量分析, 53(5) (5), 298 - 303, Japanese, Domestic magazine

  Meeting report


• Mammalian target of rapamycin complexes for cell growth comtrol devices

  吉野 健一, 大城 紀子, 米澤 一仁

  Lead, 株式会社羊土社, 01 Jul. 2005, Experimental Medicine, 23(12) (12), 1939 - 1945, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• 叡智に満ちたジャーナルへ ～編集委員長就任のご挨拶に代えて～

  吉野 健一

  Lead, 日本質量分析学会, 01 Jun. 2005, 質量分析, 53(3) (3), 179 - 182, Japanese, Domestic magazine

  Others


• Protein Identification by Mass Spectrometry

  YOSHINO Ken-ichi

  JAPANESE SOCIETY FOR BACTERIOLOGY, 25 May 2005, Nippon Saikingaku Zasshi, 60(2) (2), 397 - 417, Japanese, Domestic magazine

  [Invited]

  Introduction scientific journal


• ポストゲノム時代のLC/MS ④ MALDIでLC/MS

  吉野 健一

  Lead, 東ソー株式会社科学計測事業部, 20 Jan. 2005, HLC MAILGRAM, 102(2005-1) (2005-1), 1 - 2, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• ポストゲノム時代のLC/MS③ 象を飛ばしたエレクトロスプレーイオン化法

  吉野 健一

  Lead, 東ソー株式会社科学計測事業部, 20 Aug. 2004, HLC MAILGRAM, 101(2004-4) (2004-4), 1 - 2, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• 第52回ASMSコンファレンス(Nashville, Tennessee)レポート : キーワードはOPRY, ETD, FT-ICRそしてFLT

  吉野 健一

  Lead, 日本質量分析学会, 01 Aug. 2004, 質量分析, 52(4) (4), 257 - 261, Japanese

  Meeting report


• ポストゲノム時代のLC/MS② ゲノムに直接支配されない生体分子群の解析

  吉野 健一

  Lead, 東ソー株式会社科学計測事業部, 25 Jun. 2004, HLC MAILGRAM, 100(2004-3) (2004-3), 1 - 2, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• Mass Spectrometry-Based Protein Identification by Correlation with Sequence Database

  YOSHINO Ken-ichi, OSHIRO Noriko, TOKUNAGA Chiharu, YONEZAWA Kazuyoshi

  The progress in genome sequencing projects of a large number of organisms and the advance in mass spectrometry of protein analysis have been significant driving forces in the formation of the field proteomics. The advance in protein mass spectrometry includes development of computer algorithms that use mass spectrometric data to identify proteins in sequence databases. The computer algorithms for protein identification by correlation with sequence databases rely on the availability of constraining parameters that distinguish specific matches from all the other sequences in the database. They can be categorized into three strategies. One of strategies is called “peptide mass fingerprinting,” which is based upon correlating measured masses of peptides derived from digestion of proteins by a residue-specific protease with theoretically calculated peptide masses derived from proteins registered in sequence database. Two strategies for protein identification using tandem mass spectrometry (MS/MS) data are distinguished by demand for interpretation of product ion mass spectra. Product ion mass fingerprinting using uninterpreted MS/MS data of peptides is conceptually similar approach to peptide mass fingerprinting. SEQEUST^® and MS/MS Ions Search in MASCOT^® are the most widely used algorithms for protein identification by searching sequence database using uninterpreted product ion mass spectra. Another strategy using MS/MS data employs the search algorithm by using parameters, such as “peptide sequence tag,” found by manual inspection of product ion mass spectra.

  Lead, The Mass Spectrometry Society of Japan, 01 Jun. 2004, Journal of the Mass Spectrometry Society of Japan, 52(3) (3), 106 - 129, Japanese, Domestic magazine

  [Refereed]

  Introduction scientific journal


• ポストゲノム時代のLC/MS① ポストゲノム時代を担うLC/MS

  吉野 健一

  Lead, 東ソー株式会社科学計測事業部, 15 Apr. 2004, HLC MAILGRAM, 99(2004-2) (2004-2), 1 - 2, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• ラパマイシン標的タンパク質mTORシグナル伝達系

  徳永 千春, 大城 紀子, 吉野 健一, 原 賢太, 米澤 一仁

  秀潤社, Mar. 2004, 細胞工学, 23(3) (3), 340 - 348, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• Raptor, a binding partner of target of rapamycin

  Kazuyoshi Yonezawa, Chiharu Tokunaga, Noriko Oshiro, Ken-ichi Yoshino

  The mammalian target of rapamycin (mTOR) controls cell growth in response to amino acids and growth factors, in part by regulating p70 S6 kinase alpha (p70 alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor (regulatory associated protein of mTOR) is a 150 kDa mTOR binding protein that is essential for TOR signaling in vivo and also binds 4EBP1 and p70alpha through their respective TOS (TOR signaling) motifs, a short conserved segment previously shown to be required for amino acid- and mTOR-dependent regulation of these substrates in vivo. Raptor appears to serve as an mTOR scaffold protein, the binding of which to the TOS motif of mTOR substrates is necessary for effective mTOR-catalyzed phosphorylation. Further understanding of regulation of the mTOR-raptor complex in response to the nutritional environment would require identification of the interplay between the mTOR-raptor complex and its upstream effectors such as the protein products of tumor suppressor gene tuberous sclerosis complexes 1 and 2, and the Ras-related small G protein Rheb.

  09 Jan. 2004, Biochemical and biophysical research communications, 313(2) (2), 437 - 441, English, International magazine

  [Refereed]

  Introduction scientific journal


• mTOR integrates amino acid- and energy-sensing pathways

  Chiharu Tokunaga, Ken-ichi Yoshino, Kazuyoshi Yonezawa

  The AMP-activated protein kinase (AMPK) exists as a heterotrimetric complex comprising a catalytic alpha subunit and non-catalytic beta and gamma subunits. Under conditions of hypoxia, exercise, ischemia, heat shock, and low glucose, AMPK is activated allosterically by rising cellular AMP and by phosphorylation of the catalytic alpha subunit. The mammalian target of rapamycin (mTOR) controls cellular functions in response to amino acids and growth factors. Recent reports including our study have demonstrated the possible interplay between mTOR and AMPK signaling pathways, supporting a model in which mitochondrial dysfunction caused by the mitochondrial inhibitors or ATP depletion inhibits activation of p70 S6 kinase alpha (p70alpha), a downstream effector of mTOR, by activating AMPK. Leucine may stimulate p70alpha phosphorylation via mTOR pathway, in part, by serving both as a mitochondrial fuel through oxidative carboxylation and an allosteric activation of glutamate dehydrogenase. This hypothesis may support an idea in which leucine modulates mTOR function, in part by regulating mitochondrial function and AMPK. Further understanding of the role of mTOR in coordinating amino acid- and energy-sensing pathways would provide new insights into relationship between nutrients and cellular functions.

  09 Jan. 2004, Biochemical and biophysical research communications, 313(2) (2), 443 - 446, English, International magazine

  [Refereed]

  Introduction scientific journal


• ANALYSIS OF RECOGNITION SITE FOR SRC-SPECIFIC MONOCLONAL ANTIBODY(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan) :

  Iwasaki Tetsushi, Sato Ken-ichi, Yoshino Ken-ichi, Kosuge Keiko, Sakakibara Keiichi, Owada Koji, Yonezawa Kazuyoshi, Fukami Yasuo

  Zoological Society of Japan, 2004, Zoological science, 21(12) (12), 1285 - 1285, English


• 第51回ASMSコンファレンス(Montréal, CANADA)レポート : SARS,ノーべル賞そしてエピジェネティクス

  吉野 健一

  Lead, 日本質量分析学会, 01 Oct. 2003, 質量分析, 51(5) (5), 559 - 561, Japanese

  [Refereed]

  Meeting report


• アミノ酸と細胞内シグナル伝達－蛋白質合成を中心に

  大城 紀子, 吉野 健一, 徳永 千春, 米澤 一仁

  メディカルレビュー社, Oct. 2003, 栄養 評価と治療, 20(5) (5), 478 - 482, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• 細胞成長を司る情報伝達システムの中央情報集積装置mTOR複合体

  吉野 健一, 徳永 千春, 原 賢太, 米澤一仁

  Lead, 共立出版株式会社, 01 Aug. 2003, 蛋白質核酸酵素, 48(10) (10), 1378 - 1385, Japanese, Domestic magazine

  [Refereed][Invited]

  Introduction commerce magazine


• 暫定共通配列データベースを利用した新規タンパク質の同定

  吉野 健一, 米澤 一仁

  Lead, 医歯薬出版, 03 Aug. 2002, 医学のあゆみ, 202(5) (5), 325 - 330, Japanese

  [Invited]

  Introduction commerce magazine


• 第49回ASMS (Chicago, IL) レポート

  吉野 健一

  Lead, 01 Oct. 2001, 質量分析, 49(5) (5), 215 - 216, Japanese, Domestic magazine

  [Refereed]

  Meeting report


• 海外会員便り

  吉野健一

  Lead, Oct. 1998, 日本細菌学会関東支部ニュース, (32) (32), 7 - 7, Japanese, Domestic magazine

  [Invited]

  Introduction other


• ベロ毒素吸着剤による大腸菌O157感染症治療法

  吉野 健一, 竹田 多恵

  Lead, (株)医薬ジャーナル社, May 1997, 化学療法の領域, 13(6) (6), 1155 - 1158, Japanese

  [Invited]

  Introduction commerce magazine


• 腸管出血性大腸菌感染症の本邦臨床統計

  竹田 多恵, 吉野 健一, 内田 寛, 松田枝里子, 山形匡子

  (株)日本臨床社, Mar. 1997, 日本臨床, 55(3) (3), 693 - 699, Japanese

  Introduction commerce magazine


• Vero毒素吸着剤の開発研究の動向

  竹田 多恵, 吉野 健一, 松田 枝里子

  (株)日本臨床社, Mar. 1997, 日本臨床, 55(3) (3), 751 - 757, Japanese

  Introduction commerce magazine


• 腸管出血性大腸菌O157:H7感染症〈医学セミナー〉話題の感染症 その正しい理解と対応のために［１］

  吉野 健一, 竹田 多恵

  Lead, (株)文光堂, Feb. 1997, Quality Nursing, 3(2) (2), 191 - 195, Japanese, Domestic magazine

  [Invited]

  Introduction commerce magazine


• Amino acid sequence of a peptide that inhibits human lymphocyte proliferation induced by the superantigen Yersinia pseudotuberculosis-derived mitogen

  Ken-ichi YOSHINO, Toshifumi TAKAO, Makiko ISHIBASHI, Yuji SAMEJIMA, Yasutsugu SHIMONISHI, Tae TAKEDA

  Lead, National Institute of Health (Tokyo, JAPAN), 31 Jan. 1997, Japanese Journal of Medical Science and Biology, 49(5-6) (5-6), 256 - 257, English, International magazine

  Introduction international proceedings


• Purification and characaterization of a novel variant of the superantigen Yersinia pseudotuberculosis-derived mitogen

  Tae TAKEDA, Tandavarayan RAMAMURTY, Ken-ichi YOSHINO, Norikazu IKEDA, Yoshiko KATO

  31 Jan. 1997, Japanese Journal of Medical Science and Biology, 49(5-6) (5-6), 252 - 253, English, Domestic magazine

  Introduction international proceedings


• 1996年夏、日本で多発した腸管出血性大腸菌O157感染症

  竹田 多恵, 吉野 健一, Thandavarayan Ramamurthy, 内田 寛, 松田 枝里子, Amit Pal

  1996, 日本感染症学雑誌, 51(4) (4), 1037 - 1042, Japanese

  [Invited]

  Introduction scientific journal


• パルスフィールドゲル電気泳動によるYersinia pseudotuberculosisの分子疫学

  竹田 多惠, 吉野 健一, Thandavarayan Ramamurthy, Nair G, B, 福島 博, 大友 良光, 金子 誠二

  日水製薬(株), Jan. 1996, メディヤ サークル, 41(1) (1), 41 - 46, Japanese, Domestic magazine, Co-authored internationally

  Introduction scientific journal


• Amino Acid Sequences of the Precursor Protein and the Recombinant Toxin of a Novel Yersinia enterocolitica Heat-Stable Enterotoxin (Y-STc) that Incudes a 'Pro Region'-like Sequence in its Mature Toxin Region

  Ken-ichi YOSHINO, Xiaozhe HUANG, Hiroshi NAKAO, Tae TAKEDA

  Lead, 国立予防衛生研究所, 25 Dec. 1995, Japanese Journal of Medical Science and Biology, 48(5-6) (5-6), 283 - 284, English, International magazine

  Introduction international proceedings


• Nucleotide sequence of the gene encoding a novel superantigen (YPM) produced by Yersinia pseudotuberculosis and the geographical heterogeneity in the prevalence of the gene

  Tae Takeda, Ken-ichi Yoshino, Thandavarayan Ramamurthy, Yoshihiko Ito, Jun. Abe, Takao Kohsaka, Hiroshi Fukushima

  The National Institute of Health (Tokyo), 25 Dec. 1995, Japanese Journal of Medical Science and Biology, 48(5-6) (5-6), 261 - 262, English, International magazine

  Introduction international proceedings


• Establishment of a novel superantigen produced by Yersinia pseudotuberculosis and its association with systemic Kawasaki disease-like symptoms

  Tae Takeda, Jun Abe, Ken-ichi Yoshino, Yasuhiko Itoh, Shin-ichi Matsumoto, Yasutsugu Shimonishi, Takao Kohsaka, Noboru Kobayashi

  Elsevier Science, 01 Dec. 1995, Kawasaki Disease: Proceedings of the 5th International Kawasaki Disease Symposium, Fukuoka, Japan, 193 - 199, English, International magazine

  Introduction international proceedings


• GENETIC-ANALYSIS OF A NOVEL SUPERANTIGEN PRODUCED BY YERSINIA-PSEUDOTUBERCULOSIS AND ITS EFFECT IN ACUTE-PHASE PATIENTS

  J ABE, Y ITO, K YOSHINO, S MATSUMOTO, T TAKEDA, T KOHSAKA

  Mar. 1995, FASEB JOURNAL, 9(3) (3), A491 - A491, English

  Summary international conference


• Purification and Protein Characterization of a Novel Superantigen Produced by a Yersinia pseudotuberculosis Strain

  Ken-ichi YOSHINO, Jun ABE, Hiroshi MURATA, Toshifumi TAKAO, Takao KOHSAKA, Yasutsugu SHIMONISHI, Tae TAKEDA

  Lead, The National Institute of Health (Tokyo), 25 Dec. 1994, Japanese Journal of Medical Science and Biology, 47(5-6) (5-6), 298 - 299, English, International magazine

  Introduction international proceedings


• Purification and characterization of a superantigenic toxin produced by Yersinia pseudotuberculosis

  Tae TAKEDA, Jin ABE, Hiroshi NAKAO, Toshihiko SENBA, Ken-ichi YOSHINO, Nobuaki TAKEDA, Mutsuo TANAKA, Yasutsugu SHIMONISHI, Takao KOHSAKA, Noboru KOBAYASHI

  National Institute of Health (Tokyo, JAPAN), 10 Mar. 1994, Japanese Journal of Medical Science and Biology, 46(5-6) (5-6), 238 - 239, English, International magazine

  Introduction international proceedings


• Two novel heat-stable enterotoxins produced by yst gene-negative strains of Yersinia enterocolitica

  Ken-ichi YOSHINO, HUANG Xiaozhe, Miki MIYACHI, Toshifumi TAKAO, Hiroshi MURATA, Hiroshi NAKAO, LAI Xin-He, Tae TAKEDA, Yasutsugu SHIMONISHI

  Lead, National Institute of Health (Tokyo, JAPAN), 10 Mar. 1994, Japanese Journal of Medical Science and Biology, 46(5-6) (5-6), 236 - 237, English, International magazine

  Introduction international proceedings


• The relationship between amino acid sequences of sperm-activating peptides and the taxonomy of echinoids.

  Norio SUZUKI, Ken-ichi YOSHINO

  Last, Aug. 1992, Comparative Biochemistry and Physiology, 102B(4) (4), 679 - 690, English, International magazine

  [Refereed]

  Introduction scientific journal


• A free o-bromophenylalanine, and o-, m-, p-phenylalanine- and bromohistidine-containing sperm-activating peptides in the extracellular matrix odf sea urchin eggs

  Ken-ichi YOSHINO, Toshifumi TAKAO, Masahiko SUHARA, Yasutsugu SHIMONISHI, Norio SUZUKI

  Lead, Elsevier Science Publishers, 1992, Frontiers and New Horizons in Amino Asid Research, 315 - 320, English, International magazine

  Introduction international proceedings


• Tandem mass spectrometry of a new SAP-IIB derivative isolated from the egg jelly of the sea urchin Stomosneustes variolaris

  Ken-ichi YOSHINO, Toshifumi TAKAO, Yasutsugu SHIMONISHI, Norio SUZUKI

  Lead, 1992, Peptide Chemstry 1991, 327 - 332, English

  Summary national conference


• Further structural stady of sperm-activating peptides isolated from the egg jelly of sea urchins, Tripneustes gratilla, Pseudoboletia maculata, Heterocentrotus mammillatus and Clypeaster japonicus, using fast atom bombardment mass spectrometry

  Ken-ichi YOSHINO, Masaaki YAMAGUCHI, Norio SUZUKI, Toshifumi TAKAO, Yasutsugu SHIMONISHI, Koji NOMURA

  Lead, A. A. Balkema (Rotterdam), 1991, Biology of Echinodermata, 419 - 425, English, International magazine

  Introduction international proceedings


• Primary Structures of Bromophenylalanine-containing Sperm-activating Peptides

  Ken-ichi YOSHINO, Toshifumi TAKAO, Hideaki HORI, Masahiko SUHARA, Tetsuya KITAI, Koji NOMURA, Masaaki YAMAGUCHI, Yasutsugu SHIMONISHI, Norio SUZUKI

  Lead, Protein Research Foundation, 1991, Peptide Chemistry 1990, 171 - 176, English, International magazine, Co-authored internationally

  Summary national conference


• Taxonomical significance of respiratory stimulation of sea urchin spermatozoa by egg associated substances

  Norio SUZUKI, Ken-ichi YOSHINO, Masanori KURITA, Masaaki YAMAGUCHI, Shonan AMEMIYA

  A. A. Balkema (Rotterdam), 1988, Echinoderm Biology, 213 - 218, English, International magazine

  Introduction international proceedings

• マススペクトロメトリー関係用語集（第4版）

  吉野, 健一

  Editor, 一般社団法人日本質量分析学会, Mar. 2020, Japanese, ISBN: 9784902590906


• 日本工業規格, 高速液体クロマトグラフィー質量分析通則 JIS K 0136:2015

  Yoshino Ken-ichi

  Contributor, 一般社団法人 日本規格協会, Mar. 2015, Japanese

  Others


• タンパク質分析

  吉野 健一

  Contributor, 2.2 質量分析法によるシークエンシング, 丸善出版, Nov. 2012, Japanese, ISBN: 9784621086155


• 分析化学用語辞典

  吉野 健一

  Contributor, オーム社, Oct. 2011, Japanese, ISBN: 9784274210914


• 改訂 タンパク質実験ハンドブック : 取り扱いの基礎から機能解析まで完全網羅!

  吉野 健一

  Contributor, 第４章 質量分析法を利用したタンパク質同定法, 羊土社, Feb. 2011, Japanese, ISBN: 9784758101790


• マススペクトロメトリー関係用語集（第３版）

  吉野 健一, 内藤 康秀, 瀧浪 欣彦, 竹内 孝江, 豊田 岐聡, 益田 勝吉

  Joint editor, 日本質量分析学会, Jun. 2009, Japanese

  Dictionary or encycropedia


• Xenopus protocols: cell biology and signal transduction (Methods in Molecular Biology, 322)

  Ken-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov, Tetsushi Iwasaki, Kazuyoshi Yonezawa, Yasuo Fukami

  Contributor, 28. Signal transduction of egg fertilization : Focused proteomics on membrane/lipid rafts, Humana Press Inc., Totowa, NJ, Dec. 2005, 395-411, English, ISBN: 1588293629

  Scholarly book


• TOR : target of rapamycin (Current Topics in Microbiology and Immunology, 279)

  Kazuyoshi YONEZAWA, Ken-ichi YOSHINO, Chiharu TOKUNAGA, Kenta HARA

  Joint work, Kinase Activities Associated with mTOR, Springer, Aug. 2003, 271-282, English, ISBN: 354000534X

  Scholarly book


• 糖尿病学2003

  徳永 千春, 吉野 健一, 原 賢太, 米澤 一仁

  Contributor, アミノ酸-ｍTOR-raptorシグナル伝達系の解明とインスリン作用, 株式会社 診断と治療社, Jun. 2003, 21-31, Japanese, ISBN: 4787813161

  Scholarly book


• プロテオミクスの最新技術 (シーエムシーBOOKS 669)

  吉野健一, 米澤一仁

  Contributor, 質量分析法による配列未知蛋白質の同定：非哺乳動物モデル生物のプロテオーム解析, シーエムシー出版, Nov. 2002, 81-92, Japanese, ISBN: 4882317761

  Scholarly book


• 分子糖尿病学の進歩 : 基礎から臨床まで（2001）

  米澤一仁, 吉野健一, 原賢太

  Joint work, 2:インスリン作用の分子機構:4:ｍTORとインスリン作用, 金原出版, Mar. 2001, Japanese, ISBN: 4307510089


• The Comprehensive Sourcebook of Bacterial Protein Toxins, Second Edition

  Tae TAKEDA, Ken-ichi YOSHINO, Thandavarayan RAMAMURTY, G. Balakrish NAIR

  Joint work, Chapter 30; Heat-stable enterotoxin of Vibrio and Yersinia species, Academic Press, Aug. 1999, 545-556, English, ISBN: 0120530759

  Scholarly book


• Escherichia coli O157:H7 and other Shiga Toxin-Producing E. coli Strains

  Tae TAKEDA, Ken-ichi YOSHINO, Hiroshi UCHIDA, Norikazu IKEDA, Masako TANIMURA

  Contributor, Early use of fosfomycin for Shiga toxin-producing Escherichia coli O157 infection reduces the risk of hemolytic uremic syndrome, American Society for Microbiology, Jul. 1998, 385-387, English, ISBN: 9781555811297

  Scholarly book


• バイオロジカルマススペクトロメトリー (現代化学増刊 31)

  高尾敏文, 宮地美貴, 吉野健一, 下西康嗣

  Joint work, 6.タンパク質、6.3 修飾タンパク質, 東京化学同人, Apr. 1997, Japanese, ISBN: 480791331X


• Cytokines, Cholera and the Gut

  Ken-ichi YOSHINO, Thandavarayam RAMAMURTHY, Jun ABE, Yasuhiko ITO, G. Balakrish NAIR, Toshifumi TAKAO, Takao KOHASAKA, Yasutsugu SHIMONISHI, Tae TAKEDA

  Joint work, Chapter 35:Purification and Characterization of a Novel Superantigen Produced by Yersinia pseudotuberclosis, Ios Pr Inc, Jan. 1997, English, Co-authored internationally, ISBN: 905199298X

  Scholarly book


• Contribution to Microbiology and Immunology: Volume 13; Yersiniosis: present and future: 6th International Symposium on Yersinia, Rome, September 26-28, 1994

  Tae TAKEDA, Ken-ichi YOSHINO, Yasuhiko ITOH, Jun ABE, Yasutsugu SHIMONISHI, Takao KOHSAKA, Noboru KOBAYASHI

  Joint work, Purification and gene cloning of a superantigen (YPM) produced by a patient strain of Yersinia pseudotuberculosis, pp. 331-333, S. Karger (Basel), Sep. 1995, English, ISBN: 9783805561389

• 熱帯熱マラリア原虫の生殖母体期におけるGAP45 の分子局在解析と相互 作用分子の探索

  面田 彩馨, 伊坂 恵里奈, 松本 香乃実, 吉野 健一, 森田 将之, 橘 真由美, 高島 英造, 坪井 敬文, 鳥居 本美, 入子 英幸

  第94回日本寄生虫学会大会, Mar. 2025, Japanese, 日本寄生虫学会, 大阪大学コンベンションセンター, Japan, Domestic conference

  Oral presentation


• 前立腺癌細胞株のプロテアソーム阻害剤に対する薬剤耐性におけるPolo-like kinaseの役割

  植原 美遊, 重村 克巳, 吉野 健一, 福田 伊津子, 白井 康仁, 上田 修司

  第34回泌尿器科分子・細胞研究会, Mar. 2025, Japanese, 泌尿器科分子・細胞研究会, なんばスカイオ コンベンションホール, Japan, Domestic conference

  Poster presentation


• 質量分析用語

  吉野健一

  第53回質量分析講習会、基礎（A）コース「質量分析の基礎」, Dec. 2024, Japanese, 一般社団法人日本質量分析学会, Japan, Domestic conference

  [Invited]

  Public discourse


• 植物におけるカテキン/ビタミンE受容体の相互作用蛋白質同定

  林 大輝, 西村 侑斗, 廣瀬 梨彩, 吉野 健一, 金丸 研吾

  日本農芸化学会関西支部第533回講演会, Dec. 2024, Japanese, 公益財団法人日本農芸化学会, 神戸大学百年記念館（六甲台第2キャンパス）, Japan, International conference

  Oral presentation


• 質量分析用語

  吉野健一

  第52回質量分析講習会、基礎（A）コース「質量分析の基礎」, Aug. 2024, Japanese, 一般社団法人日本質量分析学会, Japan

  [Invited]

  Public discourse


• Identification of a Variant Protein of HSP70 Chaperone of Malaria Parasites (Plasmodium falciparum) by Mass Spectrometry and Database Searches using Extracted UniProtKB Database

  Ken-ichi Yoshino, Ayaka Omoda, Hideyuki Iriko

  第72回質量分析総合討論会, Jun. 2024, English, 一般社団法人日本質量分析学会, つくば国際会議場, Japan

  Poster presentation


• HSPB5を介した小胞体ストレス応答における小胞輸送のメカニズムの解明

  齋藤由純, 火岡優希, 吉野健一, 福田伊津子, 白井康仁, 上田修司

  第70回日本生化学会近畿支部例会, May 2024, Japanese, 日本生化学会近畿支部, 国立循環器病研究センター

  Oral presentation


• 質量分析への扉

  吉野健一

  第175回日本質量分析学会関西談話会, Mar. 2024, Japanese, 一般社団法人日本質量分析学会, 株式会社島津製作所関西支社、大阪梅田ツインタワーズ・サウス24階, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 熱帯熱マラリア原虫の未成熟生殖母体期のSBP1はPfHSP70と相互作用する

  面田彩馨, 松本香乃美, 吉野健一, 橘真由美, 石野智子, 伊藤大輔, 大槻 均, 坪井敬文, 鳥居本美, 入子英幸

  第93回日本寄生虫学会大会, Mar. 2024, Japanese, 日本寄生虫学会, 順天堂大学本郷キャンパス, Japan, Domestic conference

  Oral presentation


• 質量分析用語

  吉野健一

  第51回質量分析講習会、基礎（A）コース「質量分析の基礎」, Nov. 2023, Japanese, 一般社団法人日本質量分析学会, 大阪大学産業科学研究所, Japan, Domestic conference

  [Invited]

  Public discourse


• Identification of bacterial binding proteins to perfluorooctanoic acid

  Shunsuke Azuma, Chisa Tanaka, Miyune Nakamura, Yutaka Kobayashi, Ken-ichi Yoshino, Takuya Kakoi, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

  2023 International Joint Meeting of the 23rd International Conference on Cytochrome P450 and the 38th Annual Meeting of the Japanese Society for the Study of Xenobiotics [2023 ICCP450/JSSX], Sep. 2023, English

  Poster presentation


• 熱帯熱マラリア原虫の生殖母体期における原虫タンパク質輸送の解析

  面田彩馨, 岡田小夏, 伊坂恵里奈, 吉野健一, 橘真由美, 石野智子, 鳥居本美, 入子英幸

  第78 回日本寄生虫学会 西日本支部大会,, Sep. 2023, Japanese, 日本寄生虫学会, 愛媛大学 南加記念ホール・校友会館, Japan, Domestic conference

  Oral presentation


• 質量分析用語

  吉野健一

  第50回質量分析講習会、Aコース「質量分析の基礎」, May 2023, Japanese, 一般社団法人日本質量分析学会

  [Invited]

  Public discourse


• 細菌由来のペルフルオロオクタン酸結合タンパク質の探索

  東駿介, 田中稚紗, 中村水音, 小林豊, 吉野健一, 栫拓也, 松村千里, 中野武, 乾秀之

  第2回環境化学物質3学会合同大会, May 2023, Japanese, あわぎんホール（徳島県郷土文化会館）, Japan, Domestic conference

  Poster presentation


• A Method for Efficient and Accurate Identification of Proteins of Malaria Parasites (Plasmodium falciparum) and Human, which are Prepared from Infected Red Blood Cells, by using Mass Spectrometry

  Ken-ichi YOSHINO, Ayaka OMODA, Konatsu OKADA, Atsuko TAKEUCHI, Hideyuki IRIKO

  The 71st Annual Conference on the Mass Spectrometry Society of Japan, 2023, Osaka, May 2023, English, 日本質量分析学会, Japan, Domestic conference

  Poster presentation


• 熱帯熱マラリア原虫生殖母体期の原虫タンパク質輸送におけるSBP1 の機能解析

  面田 彩馨, 伊藤 大輔, 橘 真由美, 吉野 健一, 石野 智子, 大槻 均, 鳥居 本美, 入子 英幸

  第92回日本寄生虫学会大会, Mar. 2023, Japanese, 日本寄生虫学会, 金沢歌劇座（金沢市）, Japan, International conference, Co-authored internationally

  Oral presentation


• 質量分析用語

  吉野健一

  第49回質量分析講習会、Aコース「質量分析の基礎」, Nov. 2022, Japanese, 一般社団法人日本質量分析学会, Japan, Domestic conference

  [Invited]

  Public discourse


• 熱帯熱マラリア原虫の生殖母体期におけるPTEX複合体の解析

  岡田小夏, 伊坂恵里奈, 面田彩馨, 吉野健一, 橘真由美, 石野智子, 鳥居本美, 入子英幸

  第77回日本寄生虫学会西日本支部大会大阪大会, Sep. 2022, Japanese

  Oral presentation


• 熱帯熱マラリア原虫生殖母体期のSBP1と相互作用するタンパク質の検索

  面田彩馨, 岡田小夏, 伊坂恵里奈, 吉野健一, 橘真由美, 石野智子, 鳥居本美, 入子英幸

  第77回日本寄生虫学会西日本支部大会大阪大会, Sep. 2022, Japanese

  Oral presentation


• 熱帯熱マラリア原虫生殖母体期のSBP1と相互作用するタンパク質の検索

  面田 彩馨, 岡田 小夏, 吉野 健一, 橘 真由美, 石野 智子, 鳥居 本美, 入子 英幸

  第55回 日本原生生物学会大会, Sep. 2022, Japanese, 法政大学 小金井キャンパス, Japan, Domestic conference

  Oral presentation


• 質量分析用語

  吉野健一

  第48回質量分析講習会、Aコース「質量分析の基礎」, Jul. 2022, Japanese, 一般社団法人日本質量分析学会, Japan

  [Invited]

  Public discourse


• The actual condition of sequence databases of chicken Gallus gallus and a method for coping to identify allergen proteins derived from chicken egg yolk using mass spectrometry-based protein identification

  Ken-ichi Yoshino, Yoshiko Oda, Atsushi Fukunaga, Akiharu Kubo, Atsuko Takeuchi

  The 70th Annual Conference on Mass Spectrometry, JAPAN, Jun. 2022, Japanese, 一般社団法人日本質量分析学会, 福岡国際会議場, Japan, Domestic conference

  Poster presentation


• 熱帯熱マラリア原虫生殖母体期におけるPTEX複合体構成タンパク質の発現プロフ ァイル解析

  岡田小夏, 伊坂恵里奈, 面田彩馨, 吉野 健一, 石野智子, 橘 真由美, 鳥居本美, 高島 英造, 坪井敬文, 入子英幸

  第91回日本寄生虫学会大会, May 2022, Japanese, とかちプラザ, Domestic conference

  Poster presentation


• 熱帯熱マラリア原虫生殖母体期におけるSBP1の機能解析

  面田彩馨, 岡田小夏, 吉野健一, 橘 真由美, 石野智子, 鳥居本美, 入子英幸

  第91回日本寄生虫学会大会, May 2022, Japanese, とかちプラザ, Domestic conference

  Poster presentation


• 卵黄による食物依存性運動誘発アナフィラキシーの１例

  川村真実, 織田好子, 田中彰浩, 山田はるひ, 福永 淳, 久保亮治, 吉野健一, 木谷美湖野

  日本皮膚科学会第488回大阪地方会, Dec. 2021, Japanese, 庄田裕紀子（住友病院）, Domestic conference

  Oral presentation


• Polo-like kinase 2 plays an essential role in cytoprotection in endoplasmic reticulum stress via phosphorylation of serine 19 in HSPB5

  Yuuki Hioka, Shuji Ueda, Moeka Nishihara, Ken-ichi Yoshino, Soichiro Yamada, Minoru Yamanoue, Yasuhito Shirai

  Cell Bio 2021 Virtual-an Online ASCB|EMBO Meeting, Dec. 2021, English, The American Society for Cell Biology

  Poster presentation


• Phosphorylation of HSPB1 regulates actin-myosin contraction in muscle cells via binding to the essential light chain

  Shuji Ueda, Kunimitu Fukushima, Ken-ichi Yoshino, Soichiro Yamada, Minoru Yamanoue, Yasuhito Shirai

  Cell Bio 2021 Virtual-an Online ASCB|EMBO Meeting, Dec. 2021, English, The American Society for Cell Biology

  Poster presentation


• 質量分析用語の正しい使い方（応用編）～用語の変遷をたどりながら～

  吉野 健一

  第47回質量分析講習会, Nov. 2021, Japanese, 一般社団法人日本質量分析学会, Japan, Domestic conference

  [Invited]

  Public discourse


• 黒毛和種牛の霜降り形質に関わるコラーゲンネットワークの役割について

  細田真菜, 吉野健一, 山之上稔, 白井康仁, 上田修司

  第71回関西畜産学会大会, Oct. 2021, Japanese, 関西畜産学会, オンライン, Domestic conference

  Oral presentation


• 黒毛和種牛の遺伝子発現プロファイルを用いた筋内脂肪のパスウェイ解析

  細田 真菜, 上田 修司, 吉野 健一, 山之上 稔, 白井 康仁

  日本農芸化学会2021年度西日本・中四国・関西支部合同大会, Sep. 2021, Japanese, 公益社団法人日本農芸化学会, Domestic conference

  Oral presentation


• Usefulness of utilizing multiple spectrum libraries containing different CID product ion spectra derived from the same molecule in a library search

  Ken-ichi YOSHINO, Atsuko TAKEUCHI

  The 69th Annual Conference on Mass Spectrometry, May 2021, Japanese, 一般社団法人日本質量分析学会, オンライン, Domestic conference

  Poster presentation


• 黒毛和種の筋内脂肪パターン形成に関わる蛋白質の網羅的解析

  細田真菜, 吉野健一, 白井康仁, 山之上稔, 上田修司

  日本食肉研究会第62回大会, Mar. 2021, Japanese, 神戸国際会議場＆オンライン開催, Japan, Domestic conference

  Poster presentation


• Small heat shock protein suppresses apoptosis caused by endoplasmic reticulum stress in L6 myocytes

  Yumeno Mitani, Shuji Ueda, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai

  Cell Bio Virtual 2020, On Line ASCB | EMBO meeting, Dec. 2020, English

  Poster presentation


• Proteomic analysis of senescence-like morphology induced by DNA damage in NIH3T3 cells

  Aika Hara, Ken-ichi Yoshino, Minoru Yamanoue, Yasuhito Shirai, Shuji Ueda

  Cell Bio 2020 Virtual-an Online ASCB | EMBO Meeting, Dec. 2020, English

  Poster presentation


• Identification of aminopropyl groups bound in the C-terminal region of branched-chain polyamine synthase (BpsA) by mass spectrometry

  Ken-ichi YOSHINO, Ryota, HIDESE Shinsuke, FUJIWARA, Atsuko TAKEUCHI

  The 68th Annual Conference on Mass Spectrometry, May 2020, English, 一般社団法人日本質量分析学会, グランキューブ大阪（大阪府立国際会議場）, Japan, Domestic conference

  Poster presentation


• Analysis of disease-related biomarker protein in urine

  Atsuko TAKEUCHI, Takahiro FUJIMOTO, Ken-ichi YOSHINO, Masafumi MATSUO

  The 68th Annual Conference on Mass Spectrometry, May 2020, Japanese, 一般社団法人日本質量分析学会, Domestic conference

  Poster presentation


• 筋細胞の小胞体ストレス応答におけるHSPB5の結合分子の探索と機能解析

  西原萌華, 上田修司, 吉野健一, 竹内敦子, 山之上稔, 白井康仁

  日本農芸化学会関西支部例会（第512回講演会）, Feb. 2020, Japanese, 公益社団法人日本農芸化学会関西支部, 京都大学百周年時計台記念館 2階 国際交流ホール, Japan, Domestic conference

  Oral presentation


• Identification of novel interacting proteins of small heat shock protein HSPB5 by using BioID2 method

  Moeka Nishihara, Shuji Ueda, Ken-ichi Yoshino, Atsuko Takeuchi, Minoru Yamanoue, Yasuhito Shirai

  The American Society for Cell Biology 2019 ASCB/EMBO Meeting, Dec. 2019, English, The American Society for Cell Biology, Walter E. Washington Convention Center, United States, International conference

  Poster presentation


• BioID法を用いたHSPB5の新規相互作用分子の探索

  西原萌華, 上田修司, 吉野健一, 竹内敦子, 山之上稔, 白井康仁

  第42回日本分子生物学会年会, Dec. 2019, Japanese, マリンメッセ福岡, Japan, Domestic conference

  Poster presentation


• “TIC”, “MRM”, “magnetic sector”, “high resolution mass measurement”, “accurate mass measurement”の適切な用法と訳語（和文用語）

  吉野健一

  第27回北海道談話会・研究会、第16回質量分析技術者研究会, Oct. 2019, Japanese, 一般社団法人日本質量分析学会, 北海道大学創成科学研究棟, Japan, Domestic conference

  Oral presentation


• 低親和性ビオチン結合タンパク質Tamavidinを用いた新規ペプチド捕捉型BioID法の開発

  吉野健一, 西原萌華, 上田修司, 竹内敦子

  The Annual Meeting of the Japan Society of Histochemistry and Cytochemistry, Sep. 2019, Japanese, Japan Society of Histochemistry and Cytochemistry, The Kobe Chamber of Commerce and Industry, Domestic conference

  Poster presentation


• モノアイソトピック質量と分子量，exact massとaccurate mass, m/zとは

  吉野健一

  第15回質量分析技術者研究会, Aug. 2019, Japanese, 一般社団法人日本質量分析学会, 大阪大学バイオテクノロジー国際交流棟（吹田市）, Japan, Domestic conference

  Oral presentation


• 問題を解きながら学ぶフラグメンテーション関連基礎用語

  吉野健一

  第46回BMSコンファレンス, Jul. 2019, Japanese, 日本質量分析学会, 札幌北広島クラッセホテル（北海道北広島市）, Japan, Domestic conference

  [Invited]

  Public discourse


• 低親和性ビオチン結合タンパク質を用いたペプチド捕捉型BioID（近位依存性ビオチン標識）法による新規タンパク質間相互作用マッピング法

  吉野健一, 西原萌華, 上田修司, 竹内敦子

  第67回質量分析総合討論会, May 2019, Japanese, 一般社団法人日本質量分析学会, つくば国際会議場 エポカルつくば, Japan, Domestic conference

  Poster presentation


• バイオマーカー探索のための尿中タンパク質のプロテオーム解析

  藤本貴大, 吉野健一, 松尾雅文, 竹内敦子

  日本薬学会 第139回年会, Mar. 2019, Japanese, 公益社団法人日本薬学会, 幕張メッセ, Japan, Domestic conference

  Poster presentation


• ジアシルグリセロールキナーゼαの新規結合分子の探索

  木本裕子, 渡辺真以, 上田修司, 吉野健一, 山之上稔, 白井康仁

  日本農芸化学会関西支部例会（第507回講演会）, Feb. 2019, Japanese, 公益社団法人日本農芸会関西支部, 京都大学百周年時計台記念館, Japan, Domestic conference

  Oral presentation


• 化学修飾トリプシンスペクトルライブラリーを利用して 想定外断片の誤同定を防止する方法

  吉野 健一, 宇佐見 至, 竹内 敦子

  第24回質量分析学会北海道談話会・研究会、第150回関東談話会, Oct. 2018, Japanese, 一般社団法人日本質量分析学会, 北海道大学, Japan, Domestic conference

  Oral presentation


• RhoA結合蛋白質として同定されたSTE20 like kinaseの筋細胞の肥大化における役割

  櫛谷 晃帆, 上田 修司, 前田 愛実, 加藤 良毅, 吉野 健一, 竹内 敦子, 山之上 稔, 白井 康仁

  第91回日本生化学会大会, Sep. 2018, Japanese, 公益社団法人日本生化学会, 国立京都国際会館, Japan, Domestic conference

  Poster presentation


• kg定義改定について

  吉野健一

  第14回質量分析技術者研究会, Jul. 2018, Japanese, 質量分析技術者研究会, 奈良先端科学技術大学院大学先端科学技術研究科バイオサイエンス棟（生駒市）, Japan, Domestic conference

  Oral presentation


• 感度良く測定するためのコツは？

  吉野健一

  第14回質量分析技術者研究会, Jul. 2018, Japanese, 質量分析技術者研究会, 奈良先端科学技術大学院大学先端科学技術研究科バイオサイエンス棟（生駒市）, Japan, Domestic conference

  Oral presentation


• 質量分析の基礎 ～測定原理からLC/MS～ 「全体のまとめと効果測定」

  吉野 健一

  一般社団法人日本質量分析学会 第42回質量分析講習会, Jun. 2018, Japanese, 一般社団法人日本質量分析学会, 公益財団法人微生物化学研究会 微生物化学研究所, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析の基礎 ～測定原理からLC/MS～ 「１日目のまとめ」

  吉野 健一

  一般社団法人日本質量分析学会 第42回質量分析講習会, Jun. 2018, Japanese, 一般社団法人日本質量分析学会, 公益財団法人微生物化学研究会 微生物化学研究所, Domestic conference

  [Invited]

  Public discourse


• A novel and simple database search strategy to prevent false-positive identification by using a spectral library of unexpected peptides from chemically modified trypsin

  YOSHINO KEN-ICHI, USAMI ITARU, TAKEUCHI ATSUKO

  Mass Spectrometry and Proteomics 2018, May 2018, Japanese, 一般社団法人日本質量分析学会、日本プロオテオーム学会, Hotel Hankyu Expo Park, Japan, Domestic conference

  Poster presentation


• 大多数のプロテオミクス科学者が知らない化学修飾トリプシンの真実たち

  吉野健一

  第22回日本質量分析学会北海道談話会・研究会, Feb. 2018, Japanese, 旭川医科大学, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• HSPB1 protein increases actomyosin ATPase activity of myofibrils fraction

  Yoshitake Kato, Yoshito Kokaji, Ken-ichi Yoshino, Atsuko Takeuchi, Miroru Yamanoue, Yasuhito Shirai, Shuji Ueda

  The American Society for Cell Biology 2017 ASCB/IFCB Meeting, Dec. 2017, English, The American Society for Cell Biology, Philadelphia conventional center, Pennsylvania, International conference

  Poster presentation


• Investigation of RhoA binding proteins using BioID system

  Akiho Kushiya, Soichiro Yamada, Ken-ichi Yoshino, Yoshitake Kato, Atsuko Takeuchi, Luc A. Sabourin, Andrey V. Cybulsky, Minoru Yamanoue, Yasuhito Shirai, Shuji Ueda

  The American Society for Cell Biology 2017 ASCB/IFCB Meeting, Dec. 2017, English, The American Society for Cell Biology, Pennsylvania Convention Center, United States, International conference, Co-authored internationally

  Poster presentation


• 化学修飾トリプシンの知られざる素顔

  吉野健一, 竹内敦子

  第21回質量分析学会北海道談話会・研究会, Oct. 2017, Japanese, 一般社団法人日本質量分析学会, 北海道大学, Japan, Domestic conference

  Oral presentation


• 現場の課題を一緒に考えてみませんか？ 初心者がはまるリン酸化ペプチド分析の落とし穴

  吉野健一

  第44回BMSコンファレンス, Jul. 2017, Japanese, 一般社団法人日本質量分析学会, 長浜ロイヤルホテル, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 問題を解きながら学ぶ質量分析用語

  吉野健一

  The 44th Biological Mass Spectrometry Conference, Jul. 2017, Japanese, 日本質量分析学会, 長浜ロイヤルホテル（長浜市）, Japan, Domestic conference

  [Invited]

  Public discourse


• Strategy for analysis of the splicing regulatory factors using high-resolution mass spectrometry

  TAKEUCHI ATSUKO, TORU TAKARADA, YOSHINO KEN-ICHI, MATSUO MASAFUMI

  The 65th Annual Conference on Mass Spectrometry, May 2017, Japanese, 一般社団法人日本質量分析学会, Tsukuba International Congress Center, Japan, Domestic conference

  Poster presentation


• Inconvenient truths of chemically modified trypsin

  YOSHINO KEN-ICHI, TAKEUCHI ATSUKO

  The 65th Annual Conference on Mass Spectrometry, May 2017, Japanese, 一般社団法人日本質量分析学会, Tsukuba International Congress Center, Japan, Domestic conference

  Poster presentation


• Identification of novel RhoA binding proteins by using BioID system

  Akiho KUSHIYA, Yoshitake KATO, Soichiro YAMADA, Ken-ichi YOSHINO, Atsuko TAKEUCHI, Minoru YAMANOUE, Yasuhito SHIRAI, Shiji UEDA

  The American Society for Cell Biology 2016 ASCB/IFCB Meeting, Dec. 2016, English, Moscone Center San Francisco, California, United States, International conference, Co-authored internationally

  Poster presentation


• Identification of novel HSPB1 binding proteins in skeletal muscle

  Yoshitake KATO, Yasuto KOKAJI, Akiho KUSHIYA, Ken-ichi YOSHINO, 竹Atsuko TAKEUCHI, Minoru YAMANOUE, Yasuhito SHIRAI, Shuji UEDA

  The American Society for Cell Biology 2016 ASCB/IFCB Meeting, Dec. 2016, English, Moscone Center San Francisco, United States, International conference

  Poster presentation


• Identification of novel RhoA binding protein by usinBioID system

  櫛谷晃帆, 上田 修司, 加藤良毅, 吉野健一, 竹内敦子, 山之上稔, 白井康仁

  第39回日本分子生物学会年会, Dec. 2016, Japanese, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• 今さら聞けない質量分析用語の疑問点

  吉野健一

  第18回日本質量分析学会北海道談話会･研究会, Oct. 2016, Japanese, 一般社団法人日本質量分析学会, 北海道大学創成科学研究棟（札幌市）, Japan, Domestic conference

  Oral presentation


• 骨格筋におけるHSPB1結合蛋白質の探索とそのアクトミオシンの ATPase活性に与える影響

  Kokaji Yoshito, Kato Yoshiki, Kushiya Akiho, YOSHINO KEN-ICHI, Takeuchi Atsuko, UEDA SHUJI, YAMANOUE MINORU, SHIRAI YASUHITO

  日本農芸化学会2016年度関西支部講演会（第496回講演会）, Sep. 2016, Japanese, 公益社団法人日本農芸化学会関西支部, 滋賀県立大学（滋賀県彦根市八坂町2500）, Japan, Domestic conference

  Oral presentation


• 筋肉の増加減退に関わるRhoAの機能解析と相互作用分子の探索

  櫛谷晃帆, 上田修司, 加藤良毅, 桐村悠佑, 石政碧, 吉野健一, 竹内敦子, 山之上稔, 白井康仁

  第63回日本生化学会近畿支部例会, May 2016, Japanese, 公益社団法人日本生化学会近畿支部, 神戸薬科大学, Japan, Domestic conference

  Oral presentation


• 質量分析法を用いたスプライシング制御機構解明へのアプローチ

  寶田徹, 吉野健一, 松尾雅文, 竹内敦子

  第64回質量分析総合討論会, May 2016, Japanese, 一般社団法人日本質量分析学会, ホテル阪急エキスポパーク, Japan, Domestic conference

  Poster presentation


• 質量分析法を利用したRNA結合蛋白質の解析

  寳田 徹, 吉野 健一, 松尾 雅文, 竹内敦子

  日本薬学会 第136年会, Mar. 2016, Japanese, 公益社団法人日本薬学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• 質量分析法を利用したスプライシング制御因子の解析

  寳田 徹, 吉野 健一, 松尾 雅文, 竹内敦子

  第38回日本分子生物学会年会、第88回日本生化学会合同大会, Dec. 2015, Japanese, 神戸国際展示場, Japan, Domestic conference

  Poster presentation


• RNAスプライシングを制御する蛋白質の分析

  寳田 徹, 吉野 健一, 松尾 雅文, 竹内 敦子

  第65回日本薬学会近畿支部大会, Oct. 2015, Japanese, 公益社団法人日本薬学会関西支部, 大阪大谷大学, Japan, Domestic conference

  Oral presentation


• Identification of the splicing regulatory factors using mass spectrometry

  Toru Takarada, Ken-ichi Yoshino, Masafumi Matsuo, Atsuko Takeuchi

  SciX 2015, Sep. 2015, English, FACSS (The Federation of Analytical Chemistry and Spectroscopy Societies), Rhode Island Convention Center, Providence, RI, United States, International conference

  Poster presentation


• RNA結合蛋白質の解析

  寳田 徹, 吉野 健一, 松尾 雅文, 竹内敦子

  第42回BMSコンファレンス, Jul. 2015, Japanese, 一般社団法人日本質量分析学会, 岐阜グランドホテル, Japan, Domestic conference

  Poster presentation


• 質量分析用語基礎講座

  吉野健一

  第42回BMSコンファレンス, Jul. 2015, Japanese, 一般社団法人日本質量分析学会, 岐阜グランドホテル（岐阜市）, Japan, Domestic conference

  [Invited]

  Public discourse


• nanoLC/ESI電場型フーリエ変換質量分析法による、封入体からのタンパク質可溶化試薬TAPS-sulfonateを用いる大腸菌リコンビナントヒトインスリン精製工程の評価

  吉野 健一, 平松 貴人, 茶谷 絵理, 竹内 敦子

  第63回質量分析総合討論会, Jun. 2015, Japanese, 一般社団法人日本質量分析学会, つくば国際会議場（つくば市）, Japan, Domestic conference

  Poster presentation


• エクソン認識を制御するRNA結合蛋白質解析法の構築

  寳田 徹, 吉野 健一, 松尾 雅文, 竹内敦子

  第63回質量分析総合討論会, Jun. 2015, Japanese, 一般社団法人日本質量分析学会, つくば国際会議場（つくば市）, Japan, Domestic conference

  Oral presentation


• 高分解能LC-MS/MSを用いるRNA結合蛋白質hnRNPA1の同定

  寳田 徹, 内池 光希, 吉野 健一, 竹内 敦子

  日本薬学会第135年会, Mar. 2015, Japanese, 公益社団法人日本薬学会, デザイン・クリエイティブセンター神戸, Japan, Domestic conference

  Poster presentation


• 筋細胞におけるHSPB1の結合分子の探索とその機能解析

  小鍛治 泰斗, 上田 修司, 吉野 健一, 山之上 稔, 白井 康仁

  第87回日本生化学会大会, Oct. 2014, Japanese, 公益社団法人日本生化学会, 国立京都国際会館, Japan, Domestic conference

  Poster presentation


• PKC KO Parkinsonian syndrome model:The role of PIX phosphorylation at Ser340 and Ser583 in dopamine release.

  Shirafuji, T, Ueyama Takehiko, Yoshino Kenichi, Adachi Naoko, Takahashi Harumi, Hiramatsu, N, Ago, Y, Matsuda, T, Toda Tatsushi, Sakai, N, Saito Naoaki

  第37回日本神経科学大会, Sep. 2014, Japanese, 日本神経科学会, 横浜, Domestic conference

  Poster presentation


• Analysis of PKC substrates in nigro-striatum system:The role of bPIX phosphorylation for dopamine release.

  Shirafuji, T, Ueyama Takehiko, Yoshino Kenichi, Adachi Naoko, Takahashi Harumi, Hiramatsu, N, Ago, Y, Matsuda, T, Toda Tatsushi, Sakai, N, Saito Naoaki

  Society for Neuroscience 2014, Sep. 2014, English, Society for Neuroscience, Washington D.C., USA, International conference

  Oral presentation


• IUPAC質量分析用語集（2013勧告）におけるMSSJ用語集第3版（2009）からの主要な変更点とその注意点

  吉野健一

  第41回BMSコンファレンス, Jul. 2014, Japanese, 日本質量分析学会, 能登ロイヤルホテル（石川県志賀町）, Japan, Domestic conference

  Poster presentation


• MSSJ用語集（第3版）とIUPAC質量分析用語集2013との差分・増分について

  吉野健一

  第62回質量分析総合討論会, May 2014, Japanese, 日本質量分析学会, ホテル阪急エキスポパーク, Japan, Domestic conference

  [Invited]

  Nominated symposium


• PKCノックアウトパーキンソン症候群モデル：bPIXリン酸化のドパミン遊離での役割

  白藤俊彦, Ueyama Takehiko, Yoshino Kenichi, Adachi Naoko, 高橋英之, 香田 健, 平松直樹, 吾郷 由希夫, 松田敏夫, Toda Tatsushi, 酒井規雄, Saito Naoaki

  第55回日本神経学会学術大会, May 2014, Japanese, 日本神経学会, 福岡, Domestic conference

  Poster presentation


• マススペクトルデータ表記法

  吉野健一

  第40回BMSコンファレンス, Jul. 2013, Japanese, 日本質量分析学会, シーガイアコンベンションセンター（宮崎市）, Japan, Domestic conference

  [Invited]

  Public discourse


• 基礎用語

  吉野健一

  第33回質量分析講習会基礎コース, May 2013, Japanese, 日本質量分析学会, 千里ライフサイエンスサンタ―, Japan, Domestic conference

  [Invited]

  Public discourse


• The Functional Analysis of the Modifications of the DNA Damage Recognition Protein DDB2(2W5II-6)

  Matsumoto, S, Fischer, E. S, Yoshino, K, Thomä, N. H, Sugasawa,K

  第35回日本分子生物学会年会, Dec. 2012, Japanese, 福岡, Domestic conference

  Keynote oral presentation


• Exploration of Novel Regulatory Factors in Mammalian Nucleotide Excision Repair (1ST3-018)

  Tone,D, Yoshino,K, Iwai,S, Sugasawa,K

  第35回日本分子生物学会年会, Dec. 2012, Japanese, 福岡, Domestic conference

  Poster presentation


• 基礎用語

  吉野健一

  第32回質量分析講習会基礎コース, Nov. 2012, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• Functional studies on ubiquitylation of the DNA damage recognition protein DDB2

  Matsumoto,S, Fischer, E. S, Yoshino,K, Thomä, N. H, Sugasawa,K

  The 8th 3R Symposium (International Symposium on DNA Replication, Recombination and Repair), Nov. 2012, English, Awaji, International conference

  Public symposium


• データベース未登録タンパク質をMASCOT検索によって同定するための変法 ～鶏肉（かしわ）由来タンパク質をモデルとして～

  吉野健一

  第6回日本質量分析学会北海道談話会・研究会, Oct. 2012, Japanese, 日本質量分析学会, 北海道大学創成研究棟, Japan

  Oral presentation


• マススペクトルを正しく理解するための質量分析用語の基礎

  吉野健一

  International Mass Spectrometry Conference (IMSC) 2012特別企画・質量分析の基礎と最先端（特別勉強会）～プロダクトイオンスペクトルの解釈から薬物動態質量分析へ, Sep. 2012, Japanese, 国立京都国際会館（京都市）, International conference

  [Invited]

  Public discourse


• Role of PKCalpha-mediated cofilin phosphorylation in the degranulation from RBL-2H3 mast cells.

  Sakuma M, Shirai Y, Yoshino Kenichi, Kuramasu M, Nakamura T, Mizuno K, Hide I, Nakata Y, Saito Naoaki

  22th IUBMB & 37th FEBS, Sep. 2012, English, Spain, International conference

  Poster presentation


• 基礎用語

  吉野健一

  第31回質量分析講習会, Jun. 2012, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• 黒質線条体系におけるPKCgammaの基質の解析

  白藤 俊彦, Yoshino Kenichi, Adachi Naoko, 高橋 英之, 香田 健, 梨子田 哲明, 吾郷 由希夫, 松田 敏夫, Saito Naoaki

  第121回日本薬理学会近畿部会, Mar. 2012, Japanese, 日本薬理学会, 徳島, Domestic conference

  Oral presentation


• 基礎用語

  吉野健一

  第30回質量分析講習会, Dec. 2011, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• 新しいMSのパワーを知る、プロテオーム関連MS、Q-TOF-MS

  吉野健一

  質量分析講習会アドバンストコース, Nov. 2011, Japanese, 日本質量分析学会, 日本化学会・化学会館, Japan, Domestic conference

  [Invited]

  Public discourse


• Novel phosphorylation site(s) of cofilin by PKCalpha contributes toproper termination of histamine release

  佐久間 恵, 白井康仁, Yoshino Kenichi, 倉増真帆, 中村朋文, 水野健作, 秀 和泉, 仲田義啓, Saito Naoaki

  第84回日本生化学会, Sep. 2011, Japanese, 日本生化学会, 京都, Domestic conference

  Oral presentation


• 脊髄小脳失調症14型 (SCA14)の原因となる変異γPKCはheat shock cognate protein 70 (Hsc70)と結合し、そのリソソームへのトランスロケーションを抑制する

  関貴弘, 吉野健一, 隠地智也, 田中茂, 秀和泉, 齋藤尚亮, 酒井規雄

  第33回日本分子生物学会年会・第83回日本生化学会年会 合同大会, Dec. 2010, Japanese, 特定非営利活動法人日本分子生物学会・社団法人日本生化学会, 神戸ポートピアホテル、神戸国際会議場、神戸国際展示場, Domestic conference

  Oral presentation


• いけないマススペクトロメトリー

  吉野健一

  第6回質量分析技術者近畿ブロック研究会, Jul. 2010, Japanese, 質量分析技術者近畿ブロック研究会, 京都大学大学院薬学研究科本館, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• 質量分析用語がわかる ～イケナイ質量分析用語～

  吉野健一

  サーモフィッシャーサイエンティフィック株式会社質量分析計ユーザーズフォーラム2010（東京会場）, Jul. 2010, Japanese, サーモフィッシャーサイエンティフィック株式会社, ホテルラフォーレ東京（品川区）, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析用語がわかる ～イケナイ質量分析用語～

  吉野健一

  サーモフィッシャーサイエンティフィック株式会社質量分析計ユーザーズフォーラム2010（大阪会場）, Jul. 2010, Japanese, サーモフィッシャーサイエンティフィック株式会社, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析基礎用語

  吉野健一

  第37回BMSコンファレンス, Jul. 2010, Japanese, 日本質量分析学会, 沖縄残波岬ロイヤルホテル, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析関連用語IUPACおよびMSSJ新勧告

  吉野健一

  第8回北海道質量分析研究会、日本質量分析学会北海道地区研究・講演会, Sep. 2009, Japanese, 北海道質量分析研究会、日本質量分析学会, 北海道大学薬学部, Japan, Domestic conference

  [Invited]

  Invited oral presentation


• 質量分析基礎用語

  吉野健一

  第25回質量分析講習会基礎コース, Jun. 2009, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• マススペクトルの基礎用語

  吉野健一

  第57回質量分析総合討論会, May 2009, Japanese, 日本質量分析学会, 大阪国際交流センター（大阪市）, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 質量分析基礎用語

  吉野 健一

  第24回質量分析講習会基礎コース, Nov. 2008, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• マススペクトルの読み方

  Yoshino Ken-ichi

  第24回質量分析講習会, Nov. 2008, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（大阪府豊中市）, Domestic conference

  [Invited]

  Invited oral presentation


• 組織細胞化学におけるプロテオミクス

  吉野 健一

  第33回組織細胞化学講習会, Aug. 2008, Japanese, 日本組織細胞化学会, 神戸国際会議場, Japan, Domestic conference

  Invited oral presentation


• 間違いやすい質量分析の基礎用語

  吉野 健一

  The 35th Biological Mass Spectrometry Conference, Jul. 2008, Japanese, 日本質量分析学会, 裏磐梯ロイヤルホテル, Japan, Domestic conference

  Poster presentation


• マススペクトルの読み方 １ ～用語解説を中心に～

  Yoshino Ken-ichi

  第23回質量分析講習会, Jun. 2008, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Public discourse


• マススペクトルの読み方 ３

  Yoshino Ken-ichi

  第23回質量分析講習会, Jun. 2008, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（大阪府豊中市）, Domestic conference

  [Invited]

  Invited oral presentation


• RalAはeta PKCによるケラチノサイトの扁平化に必須である

  Shirai Yasuhito, 盛岡 祥子, 大辻 千裕, Yoshino Ken-ichi, 酒井 規雄, Ueyama Takehiko, Saito Naoaki

  第113回日本薬理学会近畿部会, Jun. 2008, Japanese, 岡山, Domestic conference

  Oral presentation


• 質量分析基礎用語・IUPAC新勧告のポイント

  内藤康秀, 瀧浪欣彦, 竹内孝江, 豊田岐聡, 益田勝吉, 吉野健一

  第56回質量分析総合討論会, May 2008, Japanese, 日本質量分析学会, つくば国際会議場エポカル, Domestic conference

  [Invited]

  Nominated symposium


• Essential terms of mass spectrometry that need to be discussed

  吉野健一, 内藤康秀, 竹内孝江, 瀧浪欣彦, 豊田岐聡, 益田勝吉

  The 56th Annual Conference on Mass Spectrometry, May 2008, Japanese, 日本質量分析学会, International Congress Center Epochal Tsukuba, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Mass-Spectrometry-based Protein Identification by Correlation with Sequence Databases and Mass Spectrometric Fragmentation

  Yoshino Ken-ichi

  The 56th Annual Conference on Mass Spectrometry, May 2008, Japanese, 日本質量分析学会, International Congress Center Epochal Tsukuba, Domestic conference

  [Invited]

  Invited oral presentation


• マススペクトルの読み方 ３

  Yoshino Ken-ichi

  第22回質量分析講習会, Nov. 2007, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（大阪府豊中市）, Domestic conference

  [Invited]

  Invited oral presentation


• マススペクトルの読み方 １ ～用語解説を中心に～

  Yoshino Ken-ichi

  第22回質量分析講習会, Nov. 2007, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Domestic conference

  [Invited]

  Public discourse


• 用語を通して学ぶ質量分析・基礎の基礎 ～目から鱗のマススペクトロメトリー@BMSコンファレンス～

  Yoshino Ken-ichi

  The 34th Biological Mass Spectrometry Conference, Jul. 2007, Japanese, 日本質量分析学会, 鳥羽シーサイドホテル, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析用語の正しい使い方

  吉野健一

  田中耕一記念質量分析研究所社内セミナー, Jun. 2007, Japanese, 株式会社島津製作所, 株式会社島津製作所（京都市）, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析用語の諸課題

  内藤康秀, 瀧浪欣彦, 竹内孝江, 豊田岐聡, 益田勝吉, 吉野健一

  第54回質量分析総合討論会, May 2007, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Current Problems of the Terms relating to Mass Spectrometry

  Yoshino Ken-ichi

  The 55h Annual Conference on Mass Spectrometry, May 2007, Japanese, 日本質量分析学会, 広島国際会議場, Domestic conference

  [Invited]

  Nominated symposium


• Identification of TBC7, a TBC (Tre-2/Bub2/Cdc16) domain protein, as a novel component of the TSC1-TSC2 tumor suppressors.

  Akio Nakashima, Yoshino Kenichi, Takafumi Miyamoto, Satoshi Eguchi, Oshiro Noriko, Kikkawa Ushio, Kazuyoshi Yonezawa

  MBSJ Forum 2006, Molecular Biology -the Next Decade-conference & Scientific, Dec. 2006, English, 名古屋国際会議場, International conference

  Oral presentation


• Phosphoproteome Analysis by LC/MSn with Linear Ion Trap Mass Spectrometer

  Tomoyo Nakano, Ken-ichi Yoshino, Naoyuki Iida, Mitsuyo Machida, Yasuhiko Bando, Seisuke Hattori

  The 4th JHUPO and the 2nd JSCP Joint Conference, Jul. 2006, Japanese, 日本ヒトプロテオーム機構第4回大会、, 東京都新宿区、京王プラザホテル, Japan, Domestic conference

  Oral presentation


• 質量スペクトルの読み方

  吉野健一

  第33回BMSコンファレンス, Jul. 2006, Japanese, 日本質量分析学会, 大津プリンスホテル, Japan, Domestic conference

  [Invited]

  Public discourse


• マススペクトルデータを用いた配列データベース検索による蛋白質同定法

  吉野健一

  神戸大学21世紀COEプログラム拠点「蛋白質のシグナル伝達機能」拠点内技術講習会, Jun. 2006, Japanese, 神戸大学21世紀COEプログラム拠点「蛋白質のシグナル伝達機能」, 神戸大学遺伝子実験センター, Japan, International conference

  Public discourse


• A database search protocol for the detection of Ser/Thr phosphorylation on proteins by LC/MSn with linear ion trap mass spectrometer

  Tomoyo NAKANO, Ken-ichi YOSHINO, Naoyuki IIDA, Mitsuyo MACHIDA, Seisuke HATTORI, Yasuhiko BAMDO

  第54回質量分析総合討論会, May 2006, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  Poster presentation


• 質量分析関連用語の課題の克服とジャーナル編集

  吉野健一

  第54回質量分析総合討論会, May 2006, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター（豊中市）, Japan, Domestic conference

  [Invited]

  Nominated symposium


• TOS and RAIP motifs of the translational regulator protein 4E-BP1 play different roles for the association with raptor and phosphorylation by mTOR in the regulation of cell size

  Satoshi Eguchi, Chiharu Tokunaga, Hidayat, S, Oshiro Noriko, Yoshino Kenichi, Kikkawa Ushio, Kazuyoshi Yonezawa

  Cold Spring Harbor Conference on PTEN Pathways, Mar. 2006, English, New York, International conference

  Oral presentation


• mTOR による４EBP1のリン酸化を介した分子間相互作用と細胞サイズの制御

  江口 賢史, 徳永 千春, Sujuti Hidayat, 大城 紀子, 吉野 健一, 吉川 潮, 米澤 一仁

  第28回日本分子生物学会年会（福岡）, Dec. 2005, Japanese, 日本分子生物学会, 福岡Yahoo! JAPANドーム, Japan, Domestic conference

  Poster presentation


• Neurospora crassaミトコンドリアリボソーム蛋白質遺伝子の同定と進化的解析

  甘翔, 有田恭子, 磯野節子, 劉茵, 北川円, 吉野健一, 米澤一仁, 磯野克己

  第28回日本分子生物学会年会（福岡）, Dec. 2005, Japanese, 日本分子生物学会, 福岡Yahoo! JAPANドーム, Japan, Domestic conference

  Poster presentation


• 新しい「切り口」で攻めるプロテオーム解析

  吉野健一

  Thermo Electron Users' Forum 2005 製薬・ライフサイエンスLC/MSユーザーズフォーラム（大阪会場）, Nov. 2005, Japanese, サーモエレクトロン株式会社, 千里ライフサイエンスセンター

  Public discourse


• Biochemical and morphological analysis of the interaction of oligodendrocyte membrane rafts with actin filaments

  Katsutoshi TAGUCHI, Koji YOSHINAKA, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA, Shohei MAEKAWA

  第78回日本生化学会大会（神戸）, Oct. 2005, English, 神戸国際会議場、神戸国際展示場、神戸ポートピアホテル, Japan, Domestic conference

  Poster presentation


• Suppression of the mTOR-raptor signaling pathway by the inhibitor of heat shock protein 90 geldanamycin.

  Goh OHJI, Suyuti HIDAYAT, Akio NAKASHIMA, Ken-ichi YOSHINO, Noriko OSHIRO, Satoshi EGUCHI, Ushio KIKKAWA, Kazuyoshi YONEZAWA

  第78回日本生化学会大会（神戸）, Oct. 2005, English, 神戸, Japan, Domestic conference

  Oral presentation


• 質量分析による蛋白質同定法

  吉野健一

  第23回日本細菌学会技術講習会, Sep. 2005, Japanese, 日本細菌学会, 千里ライフサイエンスセンター、島津製作所本社, Japan, Domestic conference

  [Invited]

  Public discourse


• マススペクトルデータを利用したデータベース検索による蛋白質同定の基礎

  吉野健一

  第39回千里ライフサイエンスセンター技術講習会「プロテオミクス技術講習会、MALDI、ESI、MS/MS、ナノLC、データ解析」, Jul. 2005, Japanese, 財団法人千里ライフサイエンス振興財団, 千里ライフサイエンスセンター, Japan, Domestic conference

  [Invited]

  Public discourse


• マススペクトルの読み方２

  吉野健一

  質量分析講習会基礎コース, Jun. 2005, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター, Japan, Domestic conference

  [Invited]

  Public discourse


• mTORシグナリングに対する分子シャペロンHsp90の寄与

  Tsuyoshi Ooji, Sujuti Hidayat, Akio Nakashima, Kenichi Yoshino, Noriko Oshiro, Ushio Kikkawa, Kazuyoshi Yonezawa

  第52回日本生化学会近畿支部例会, May 2005, Japanese, Kobe, Domestic conference

  Oral presentation


• ナノエレクトロスプレーイオン化四重極飛行時間型タンデム質量分析法を用いた分子量6,000を超える高分子量ペプチドのアミノ酸配列解析:in-house配列データベース検索エンジンの新たな利用法

  吉野健一, 米原久美子, 大城紀子, 川俣朋子, 磯野克己, 田村厚夫, 米澤一仁

  第53回質量分析総合討論会（さいたま）, May 2005, Japanese, 日本質量分析学会, 大宮ソニックシティ, Japan, Domestic conference

  Poster presentation


• 質量分析法による新規蛋白質の探索とその機能解析

  吉野健一

  山口大学大学院医学研究科大学院セミナー, May 2005, Japanese, 山口大学大学院医学研究科, 山口大学大学院医学研究科, Japan, Domestic conference

  [Invited]

  Public discourse


• Molecular Identification and Immunochemical Characterization of Src Tyrosine Kinase Xenopus Eggs

  Tetsushi IWASAKI, Ken-ichi SATO, Ken-ichi YOSHINO, Keiko KOSUGE, Keiichi SAKAKIBARA, Zhize Ou, Yasushi UEDA, Koji OWADA, Kazuyoshi YONEZAWA, Yasuo FUKAMI

  第27回日本分子生物学会年会, Dec. 2004, 日本分子生物学会, 神戸ポートアイランド, Domestic conference

  Poster presentation


• mTOR複合体を構成するWDリピート蛋白質mLST8の機能解析

  中嶋昭雄, 岡本澄子, 徳永千春, 吉野健一, 川西一平, 江口賢史, 大城紀子, 米澤一仁

  第27回日本分子生物学会年会, Dec. 2004, Japanese, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  Poster presentation


• mTORを中枢としたアミノ酸情報感知伝達／細胞成長制御システムを担う分子探索

  吉野健一, 大城紀子, 高橋里菜子, 江口賢史, 中嶋昭雄, 谷村圭子, 大路剛, 徳永千春, 米澤一仁

  第27回日本分子生物学会年会, Dec. 2004, Japanese, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  Poster presentation


• The functions and phylogenetic correspondence of mitochondrial ribosomal proteins of Neurospora crassa

  Kyoko ARITA, Xiang Gan, Modoka KITAGAWA, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA, Akihiro KATO, Hirokazu INOUE, Katsumi ISONO

  The 27th Annual Meeting of the Molecular Biology Society of Japan, Dec. 2004, Japanese, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  Poster presentation


• mTORを介し細胞機能を制御するシグナル伝達系の研究

  米澤一仁, 大城紀子, 中嶋昭雄, 江口賢史, 徳永千春, 吉野健一

  第27回日本分子生物学会年会, Dec. 2004, Japanese, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  [Invited]

  Nominated symposium


• マススペクトルの読み方２

  吉野健一

  質量分析講習会基礎コース, Nov. 2004, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター, Japan, Domestic conference

  [Invited]

  Public discourse


• エレクトロスプレーイオン化-四重極-飛行時間型質量分析計の魅力

  吉野健一

  ジャスコインタナショナルプロテオミクスセミナー2004（大阪会場）, Nov. 2004, Japanese, ジャスコインタナショナル株式会社, 大阪国際会議場「グランキューブ大阪」

  [Invited]

  Public discourse


• エレクトロスプレーイオン化-四重極-飛行時間型質量分析計の魅力

  吉野健一

  ジャスコインタナショナルプロテオミクスセミナー2004（東京会場）, Nov. 2004, Japanese, ジャスコインタナショナル株式会社, 主婦会館「プラザエフ」, Japan, Domestic conference

  [Invited]

  Public discourse


• Molecular identification of uroplakin III as a raft protein that is tyrosine-phosphorylated upon fertilization of Xenopus eggs

  Yasuo FUKAMI, Keiichi SAKAKIBARA, Tetsushi IWASAKI, Ken-ichi YOSHINO, Ken-ichi SATO, Kazuyoshi YONEZAWA

  第77回日本生化学会大会, Oct. 2004, Japanese, 日本生化学会, パシフィコ横浜

  Nominated symposium


• Identification and Functional Analysis of Phosphorylation sites of a Novel Protein Raptor which is an Essential Scaffold Protein for mTOR Signaling

  Ippei KAWANISHI, Ken-ichi YOSHINO, Noriko OSHIRO, Akio NAKASHIMA, Kenta HARA, Chiharu TOKUNAGA, Kazuyoshi YONEZAWA

  第77回日本生化学会, Oct. 2004, English, 日本生化学会, パシフィコ横浜, Domestic conference

  Nominated symposium


• Binding between 4EBP1 and raptor is regulated by mTOR phosphorylation of 4EBP1

  Satoshi EGUCHI, Chiharu TOKUNAGA, Noriko OSHIRO, Akio NAKASHIMA, Kenta HARA, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA

  第77回日本生化学会大会, Oct. 2004, Japanese, 日本生化学会, パシフィコ横浜, Japan, Domestic conference

  Nominated symposium


• RNA翻訳制御スイッチとして働くラパマイシン標的蛋白mTORシグナルの研究

  米澤一仁, 江口賢史, 徳永千春, 大城紀子, 中嶋昭雄, 吉野健一

  第6回RNAミーティング／第6回日本RNA学会年会（熊本）, Aug. 2004, Japanese, 日本RNA学会, 熊本テルサ, Domestic conference


• Dissociation of raptor from mTOR is a mechanism of rapamaycin-induced inhibition of mTOR function

  Noriko OSHIRO, Ken-ichi YOSHINO, Sujuti HIDAYAT, Chiharu TOKINAGA, Kenta HARA, Satoshi EGUCHI, Joseph AVRUCH, Kazuyoshi YONEZAWA

  Gordon Research Conference "Second Messengers and Protein Phosphorylation", Jun. 2004, English, New Hampshire, United States, International conference, Co-authored internationally

  Poster presentation


• 配列データベース検索による蛋白質の同定

  吉野健一

  質量分析講習会応用コース１「プロテインマススペクトロメトリー」, Jun. 2004, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター, Japan, Domestic conference

  [Invited]

  Public discourse


• ESTコンセンサス配列データベースを用いた新規チロシンリン酸化蛋白質の同定

  吉野健一, 佐藤賢一, 大城紀子, 榊原圭一, 徳永千春, 深見泰夫, 米澤一仁

  第52回質量分析総合討論会, Jun. 2004, Japanese, 日本質量分析学会, 名古屋市工業研究所, Japan, Domestic conference

  Oral presentation


• Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function

  Noriko OSHIRO, Ken-ichi YOSHINO, Chiharu TOKUNAGA, Kenta HARA, Satoshi EGUCHI, J. Avruch, Kazuyoshi YONEZAWA

  第57回日本細胞生物学会大会, May 2004


• Mass Spectrometric Identification of a Novel Egg Plasma Membrane Rafts-Associated Protein that is Tyrosine-Phosphorylated upon Fertilization

  Ken-ichi YOSHINO, Ken-ichi SATO, Noriko OSHIRO, Keiichi SAKAKIBARA:Chiharu TOKUNAGA, Yasuo FUKAMI, Kazuyoshi YONEZAWA

  52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, English, Gayload Ppryland Hotel, Nashville, TN, United States, International conference

  Poster presentation


• The mammalian target of rapamycin (mTOR) partner, raptor binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.

  EGUCHI, S, TOKUNAGA, C, NOJIMA, H, OSHIRO, N, YOSHINO Kenichi, HARA, K, YONEZAWA Kazuyoshi

  Kobe University The 21st Century COE Program "Signaling Mechanisms by Protein Modification Reactions", The 1st Iternational Meeting "Conference on Lipid Messenger Signaling", Jan. 2004, English, Kobe University, Japan, International conference

  Oral presentation


• Mass spectrometric identification and functional analysis of novel protein components of mTOR (mammalian target of rapamicin ) complex

  Ken-ichi YOSHINO, Chiharu TOKUNAGA, Kazuyoshi YONEZAWA

  The 8th Korea-Japan Cancer Research Workshop, Dec. 2003, English, Okinawa Miyako Hotel (Naha, Okinawa, Japan), International conference

  [Invited]

  Poster presentation


• RapamicinによるmTOR (mammalian target of rapamysin) シグナル抑制機構

  大城 紀子, 吉野 健一, 徳永 千春, 江口 賢史, 米澤 一仁

  第26回日本分子生物学会年会（神戸）, Dec. 2003, Japanese, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  Oral presentation


• Molecular identification of Xenopus egg uroplakin III that localizes to rafts and is tyrosine-phosphorylated upon fertilization.

  Keiichi SAKAKIBARA, Ken-ichi YOSHINO, Noriko OSHIRO, Shino HIRAHARA, Y. NISHIHIRA, T. FUKUDA, A. K. M. Mahbub HASSAN, Tetsushi IWASAKI, Ken-ichi SATO, Kazuyoshi YONEZAWA, Yasuo FUKAMI

  第26回日本分子生物学会年会（神戸）, Dec. 2003, English, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  Oral presentation


• ミトコンドリアリボソーム蛋白質遺伝子の構造と機能：比較的解析と進化的意義

  有田 恭子, 甘 翔, 吉野 健一, 米澤 一仁, 加藤 晃弘, 井上 弘一, 北川 円

  第26回日本分子生物学会年会（神戸）, Dec. 2003, Japanese, 日本分子生物学会, 神戸ポートアイランド, Japan, Domestic conference

  Poster presentation


• The mammalian target of rapamycin (mTOR) partner, raptor binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif

  Satoshi EGUCHI, Chiharu TOKUNAGA, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA

  The New Frontier of RNA (RNA 2003 Kyoto), Nov. 2003, English, International conference


• 配列データベース検索による蛋白質の同定

  吉野健一

  質量分析講習会応用コース１「プロテインマススペクトロメトリー」, Nov. 2003, Japanese, 日本質量分析学会, 千里ライフサイエンスセンター, Japan, Domestic conference

  [Invited]

  Public discourse


• Direct binding and phosphorylation of two purkinje cell-enriched kinese, DGKγ and γPKC, regulate their spatio-tenporal activities

  Yasuto MOCHIDA, Yasuhito SHIRAI, Takehiro MATSUBARA, Masamitsu KURIYAMA, Noriko OSHIRO, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA, Yoshitaka ONO, Naoaki SAITO

  Neuroscience 2003, 33rd Annual Meeting of Society for Neuroscience, Nov. 2003, English


• Cellular signaling through an mTOR- containing complex.

  Kazuyoshi YONEZAWA, Chiharu TOKUNAGA, Kenta HARA, Ken-ichi YOSHINO

  第76回日本生化学会大会, Oct. 2003, English, 日本生化学会, パシフィコ横浜, Japan, Domestic conference

  [Invited]

  Nominated symposium


• Functional and Moleculer Interaction between DGKγ and γPKC

  Yasuto MOCHIDA, Yasuhito SHIRAI, Takehiro MATSUBARA, Masamitsu KURIYAMA, Noriko OSHIRO, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA, Yoshitaka ONO, Naoaki SAITO

  第76回日本生化学会大会, Oct. 2003, English, 日本生化学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• The seven WD-repeats protein Gable is one of components of mTOR complex.

  Sumiko OKAMOTO, Ken-ichi YOSHINO, Chiharu TOKUNAGA, Noriko OSHIRO, Kenta HARA, Joseph ABRUCH, Kazuyoshi YONEZAWA

  第76回日本生化学会大会, Oct. 2003, English, 日本生化学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• mTOR binding protein, raptor, binds to mTOR substrates via TOS motif.

  Satoshi EGUCHI, Chiharu TOKUNAGA, Hiroki NOJIMA, Noriko OSHIRO, Ken-ichi YOSHINO, Kenta HARA, Kazuyoshi YONEZAWA

  第76回日本生化学会大会, Oct. 2003, English, 日本生化学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• MALDI-TOF-MSとMascotを用いたペプチドマスフィンガープリンティング（PMF）法による蛋白質同定法

  吉野健一

  神戸大学21世紀COEプログラム拠点「蛋白質のシグナル伝達機能」拠点内技術講習会, Oct. 2003, Japanese, 神戸大学21世紀COEプログラム拠点「蛋白質のシグナル伝達機能」, 神戸大学バイオシグナル研究センター, Japan, International conference

  Public discourse


• 質量分析法と配列データベースを利用した蛋白質の同定

  吉野健一

  第30回BMSコンファレンス, Jul. 2003, Japanese, 日本質量分析学会, 淡路夢舞台国際会議場, Japan, Domestic conference

  [Invited]

  Public discourse


• 質量分析を用いたアミロイド線維の最終形態を決定付ける中間体のキャラクタリゼーション

  米原 久美子, 田中 修平, 吉野 健一, 米澤 一仁, 田中 厚夫

  第3回日本蛋白質化学会年会, Jun. 2003, Japanese, 日本蛋白質化学会, 札幌コンベンションセンター（札幌市）, Domestic conference

  Poster presentation


• 分岐鎖アミノ酸シグナル伝達系の中枢分子mTORに結合し、その活性を制御する新規分子raptorの同定と機能解析

  吉野健一

  第39回日本肝臓学会総会サテライトシンポジウム第5回AJINOMOTO Award受賞記念講演会, May 2003, Japanese, 日本肝臓学会, 福岡国際会議場, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 質量分析法によるアミロイド線維形成における構造単位の同定

  米原 久美子, 田中 修平, 吉野 健一, 米澤 一仁, 田村 厚夫

  第51回質量分析総合討論会, May 2003, Japanese, 日本質量分析学会, 独立行政法人産業技術総合研究所（つくば本部・つくばセンター）, Japan, Domestic conference

  Oral presentation


• mTOR結合分子raptorはTOSmtifを介してmTOR基質蛋白と結合する

  江口 賢史, 野島 洋樹, 徳永 千春, 大城 紀子, 吉野 健一, 原 賢太, 米澤 一仁

  第4回PIKPK研究会, Apr. 2003, Japanese, 神戸大学, Japan, Domestic conference

  Oral presentation


• Crosstalk between energy sensing system and mTOR signaling pathway

  Chiharu TOKUNAGA, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA

  Conference on Patho-Physiological aspects of BCAA, Mar. 2003, English, Takanawa Prince Hotel, Tokyo, Japan, International conference

  [Invited]

  Invited oral presentation


• Identification and Characterization of Raptor, a novel binding partner of mTOR

  Kazuyoshi YONEZAWA, Chiharu TOKUNAGA, Ken-ichi YOSHINO

  Conference on Patho-Physiological aspects of BCAA, Mar. 2003, English, Takanawa Prince Hotel, Tokyo, Japan, International conference

  [Invited]

  Invited oral presentation


• ヒトプロテオミクス研究のためのESTコンセンサス配列データベースの有効利用

  吉野健一, 原賢太, 大城紀子, Sujuti Hidayat, 徳永千春, 米澤一仁

  第1回ヒトプロテオーム学会, Feb. 2003, Japanese, 産業技術総合研究所共用講堂（つくば市）, Japan, Domestic conference

  Oral presentation


• 質量分析法とコンセンサス配列データベースを利用する新規蛋白質の探索

  吉野健一, 米澤一仁

  大阪大学蛋白質研究所セミナー、プロテオーム・ペプチドーム最前線 ー解析法の実際と応用ー, Feb. 2003, Japanese, 大阪大学蛋白質研究所, 大阪大学蛋白質研究所, Japan, Domestic conference

  [Invited]

  Nominated symposium


• ミトコンドリアリボソームの比較解析：構成と機能の進化

  甘翔, 北川円, 吉野健一, 米澤一仁, 加藤晃弘, 井上弘一, 礒野克己

  第25回日本分子生物学会年会, Dec. 2002, Japanese, 日本分子生物学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• 新規mTOR結合蛋白質p150^RaptorのmTOR-4EBP1 Signalingにおける機能の解析

  丸木佳子, 原賢太, Sujuti Hidayat, 吉野健一, 徳永千春, 米澤一仁

  第25回日本分子生物学会年会, Dec. 2002, Japanese, 日本分子生物学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• 新規mTOR結合蛋白質p150^RaptorのmTOR-p70^S6K Signalingにおける機能の解析

  Sujuti Hidayat, 原賢太, 丸木佳子, 吉野健一, 徳永千春, 米澤一仁

  第25回日本分子生物学会年会, Dec. 2002, Japanese, 日本分子生物学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• アミノ酸バランスを感知して細胞成長を制御するmTOR Signaling

  米澤一仁, 吉野健一, 徳永千春, 原賢太

  第25回日本分子生物学会年会, Dec. 2002, Japanese, 日本分子生物学会, パシフィコ横浜, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 新規mTOR結合蛋白質p150^Raptorの精製と質量分析法による同定・構造解析

  吉野健一, 原賢太, 大城紀子, 丸木佳子, Sujuti HIDYAT, 徳永千春, 米澤一仁

  第25回日本分子生物学会年会, Dec. 2002, Japanese, 日本分子生物学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• mTOR-scaffold蛋白p150-Raptorのクローニングとインスリン作用における役割

  原賢太, 吉野健一, 徳永千春, 永田正男, 横野浩一, 米澤一仁

  第14回分子糖尿病シンポジウム, Dec. 2002, Japanese, ホテルオオサカサンパレス, Japan

  Oral presentation


• プロテオミクスにおけるコンセンサス配列データベースの有効利用

  吉野健一

  Infocomプロテオミクスセミナー（大阪会場）, Nov. 2002, Japanese, インフォコム株式会社, 梅田スカイビル, Japan, Domestic conference

  [Invited]

  Public discourse


• ウシ視細胞ラフト様膜分画に局在する脂質と蛋白質 : 脊椎動物視細胞のraftとsignalplex

  林 文夫, 劉 涵, 義山 航, 吉野 健一, 米澤 一仁, 妹尾 圭司

  日本生物物理学会第40回年会, Nov. 2002, Japanese, 日本生物物理学会, 名古屋大学東山地区（名古屋市千種区不老町）, Japan, Domestic conference

  Oral presentation


• Activity of Xenopus Src kinase in the course of maturation, fertilization and early embryonic cell cycles

  Alexander A.TOKUMAKOV, Ken-ichi SATO, Tetsushi IWASAKI, Ken-ichi YOSHINO, Kazuyoshi YONEZAWA, Yasuo FUKAMI, Mikako SHIROUZU, Shigeyuki YOKOYAMA

  FEBS 2002 Meeting, Oct. 2002, English, Istambul, Turkey, International conference


• プロテオミクスと質量分析 ～核酸配列データベースを用いた未知蛋白質の探索～

  吉野健一

  CBI学会第222回研究講演会「創薬と質量分析の進歩」, Oct. 2002, Japanese, 情報計算化学生物学会（CBI学会）, 日本化学会化学会館, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 質量分析法とヒト暫定共通配列データベースを用いた新規mTOR結合蛋白質p150^TSWの同定と構造解析

  吉野健一, 原賢太, 大城紀子, Sujuti Hidayat, 徳永千春, 米澤一仁

  第75回日本生化学会大会, Oct. 2002, Japanese, 日本生化学会, 国立京都国際会館, Japan, Domestic conference

  [Invited]

  Nominated symposium


• 新規mTOR結合蛋白質p150TSWの精製とその機能解析

  丸木佳子、原賢太、吉野健一、大城紀子、Sujuti Hidayat、徳永千春、米澤一仁

  第75回日本生化学会大会（京都）, Oct. 2002, Japanese, 日本生化学会, 国立京都国際会館, Japan, Domestic conference

  Poster presentation


• mTORシグナル伝達経路とAMP-activated kinaseの相互作用の解析

  木村直樹, 徳永千春, 原賢太, 吉野健一, 三村治, 米澤一仁

  第75回日本生化学会大会（京都）, Oct. 2002, Japanese, 日本生化学会, 国立京都国際会館, Japan, Domestic conference

  Poster presentation


• 哺乳細胞において細胞成長を司るシグナル伝達分子群のプロテオーム解析と機能同定

  米澤一仁, 吉野健一, 徳永千春

  文部科学省科学研究費特定領域研究「ゲノム」４領域、2002年度合同班会議, Aug. 2002, Japanese, 神戸ポートピアホテル, Japan, Domestic conference


• Biosignal Mass Spectrometry

  吉野健一

  第29回BMSコンファレンス, Jul. 2002, Japanese, 日本質量分析学会, 八ヶ岳ロイヤルホテル, Japan, Domestic conference

  [Invited]

  Public symposium


• Crosstalk between mTOR and AMP-activated protein kinase

  Naoki KIMURA, Chiharu TOKUNAGA, Ken-ichi YOSHINO, Kenta HARA, Bruce E. KEMP, Lee A. WITTERS, Osamu MIMURA, Kazuyoshi YONEZAWA

  Gordon Research Conference, Second Messengers & Protein Phosphorylation, Jun. 2002, English, Kimball Union Academy, Meriden, NH, United States, International conference


• Identification of p150^TSW, a Target of Rapamycin (TOR)-Scaffold Protein containing WD-repeats

  Kazuyoshi YONEZAWA, Noriko OSHIRO, Chiharu TOKUNAGA, Yoshiko MARUKI, Xiaomeng LONG, Ken-ichi YOSHINO, Sujuti HIDAYAT, Joseph AVRUCH, Kenta HARA

  Gordon Research Conference, Second Messengers and Protein Phosphorylation, Jun. 2002, English, Kimball Union Academy, Meriden, NH, United States, International conference, Co-authored internationally


• The Complete Amino Acid Sequence Determination of a Novel Protein by Mass Spectrometry with the Tentative Human Consensus Sequence Database

  Ken-ichi YOSHINO, Kenta HARA, Noriko OSHIRO, Sujuti HIDAYAT, Chiharu TOKUNAGA, Kazuyoshi YONEZAWA

  50th ASMS Conference on Mass Spectrometry and Allied Topics, Jun. 2002, English, American Society for Mass Spectrometry, Orlando, Florida, United States

  Poster presentation


• 新規mTOR結合蛋白（p150^TORAP）の同定とインスリン作用における役割

  原賢太, 吉野健一, 徳永千春, 米澤一仁

  第45回日本糖尿病学会年次学術集会, May 2002, Japanese, 東京国際フォーラム（東京都千代田区丸の内三丁目5番1号）, Japan, Domestic conference

  Poster presentation


• Nano-ESI-Q-TOF-MSを用いた相同性の高い複数の蛋白質からなる混合物の解析

  吉野健一, 佐藤賢一, 岩崎哲史, 深見泰夫, 米澤一仁

  第50回質量分析総合討論会, May 2002, Japanese, 日本質量分析学会, 京都工芸繊維大学松ヶ崎キャンパス, Domestic conference

  Poster presentation


• Nano-ESI-Q-TOF-MSを用いたmammalian target of rapamycin (mTOR)のin vivoリン酸化部位の同定と解析

  大城紀子, 吉野健一, 原賢太, 徳永千春, 米澤一仁

  第50回質量分析総合討論会, May 2002, Japanese, 日本質量分析学会, 京都工芸繊維大学松ヶ崎キャンパス, Japan, Domestic conference

  Oral presentation


• Usefulness of the Tentative Human Consensus Sequence Database in Protein Identification by Mass Spectrometry

  吉野健一, 原賢太, 大城紀子, Sujuti Hydayat, 米澤一仁

  第50回質量分析総合討論会, May 2002, Japanese, 日本質量分析学会, 京都工芸繊維大学松ヶ崎キャンパス, Japan, Domestic conference

  Oral presentation


• プロテオミクス的手法によるゼノパス卵受精成立の場の解析

  榊原圭一, 佐藤賢一, 吉野健一, 岩崎哲史, 小西雅子, 西平友介, 米澤一仁, 深見泰夫

  第24回日本分子生物学会年会（横浜）, Dec. 2001


• ゼノパス卵SrcファミリーチロシンキナーゼXykの構造解析と発現

  岩崎哲史, 佐藤賢一, 吉野健一, 板倉修二, 米澤一仁, 大和田幸嗣, 深見泰夫

  第24回日本分子生物学会年会（横浜）, Dec. 2001


• AMP-activated protein kinaseによるp70 S6 kinase活性制御機構の解析

  木村直樹, 徳永千春, 吉野健一, 原賢太, 三村治, 米澤一仁

  第24回日本分子生物学会年会（横浜）, Dec. 2001


• 質量分析法によるmTORリン酸化部位の解析

  大城紀子, 吉野健一, 原賢太, 徳永千春, 米澤一仁

  第24回日本分子生物学会年会（横浜）, Dec. 2001


• 酵母の出芽部位に局在するElm1タンパク質の機能解析

  川俣朋子, 堀江哲郎, 吉野健一, 大城紀子, 米澤一仁, 磯野克己

  第24回日本分子生物学会年会（横浜）, Dec. 2001


• 出芽酵母のミトコンドリアリボソーム蛋白質遺伝子の同定と解析

  甘翔, 北川円, 吉野健一, 大城紀子, 米澤一仁, 磯野克己

  第24回日本分子生物学会年会（横浜）, Dec. 2001


• ラパマイシン標的蛋白質mTORを介したシグナル伝達系の研究

  米澤一仁, 吉野健一, 徳永千春, 原賢太

  第24回日本分子生物学会年会、ワークショップ（横浜）, Dec. 2001


• 新しいMSのパワーを知る、プロテオーム関連MS、Q-TOF-MS

  吉野健一

  質量分析講習会アドバンストコース「MSによるプロテオーム研究立ち上げに何が必要か」, Nov. 2001, Japanese, 日本質量分析学会, Domestic conference

  [Invited]

  Public discourse


• 蛋白合成系と蛋白質分解系を制御する分子TORを介した情報伝達機構

  米澤一仁, 吉野健一, 徳永千春, 原賢太

  第74回日本生化学会大会（京都）, Oct. 2001, Japanese, 日本生化学会, 国立京都国際会館, Japan, Domestic conference

  [Invited]

  Nominated symposium


• ロイシン誘導体Ac-Leu-NH2を用いたアミノ酸-mTORシグナル伝達機構の解析

  スユティ・ヒダヤット, 原賢太, 徳永千春, 吉野健一, 米澤一仁

  第74回日本生化学会大会（京都）, Oct. 2001, Japanese, 日本生化学会, 国立京都国際会館

  Poster presentation


• 哺乳動物におけるシグナル伝達機構解明のためのプロテオーム解析の基盤研究

  米澤一仁, 原賢太, 吉野健一

  文部科学省科学研究費特定領域研究C「ゲノム」４領域、2001年度合同班会議, Aug. 2001, Japanese, 神戸ポートピアホテル


• 酵母の出芽部位に局在するElm1タンパク質の機能解析

  川俣朋子, 堀江哲郎, 妹尾彰宏, 吉野健一, 大城紀子, 米澤一仁, 磯野克己

  酵母遺伝学フォーラム第34回研究報告会（京都）, Jul. 2001


• アミノ酸によるp70S6キナーゼの活性化に対するmTORとPDK1依存性シグナルの役割

  原賢太, 村上未玲, 徳永千春, 吉野健一, ダリオ アレッシ, 米澤一仁

  第54回日本細胞生物学会大会（岐阜）, May 2001, Japanese


• 質量分析法によって見出されたmTORリン酸化部位の解析

  谷口直子, 吉野健一, 大城紀子, 原賢太, 米澤一仁

  第54回日本細胞生物学会大会（岐阜）, May 2001, Japanese


• mTORを介した情報伝達系の研究

  米澤一仁, 吉野健一, 徳永千春, 原賢太

  第54回日本細胞生物学会大会（岐阜）, May 2001, Japanese


• ウェルシュ菌のプロテオーム解析

  清水健, 嶋謙介, 吉野健一, 米澤一仁, 林英生, 清水徹

  第3回ワークショップ「微生物ゲノム研究のフロンティア」, Feb. 2001, Japanese, 文部省科学研究費補助金・特定領域研究『統合ゲノム』, かずさアカデミアホール（千葉県木更津市）, Japan, Domestic conference

  Poster presentation


• Proteomics analysis of Clostridium perfringens extracelluar protein

  嶋謙介, 清水健, 吉野健一, 米澤一仁, 林英生, 清水徹

  第23回日本分子生物学会年会（神戸）, Dec. 2000, Japanese, 日本分子生物学会, 神戸国際展示場, Domestic conference

  Poster presentation


• Proteomics analysis of VirR/VirS regulated-protein in Clostridium perfringens

  清水健, 嶋謙介, 吉野健一, 米澤一仁, 林英生, 清水徹

  第23回日本分子生物学会年会（神戸）, Dec. 2000, Japanese, 日本分子生物学会, 神戸国際展示場, Domestic conference

  Poster presentation


• Leucine誘導体L-Leu8MAPによるmTOR情報伝達系の活性化

  スユティ・ヒダヤット, 吉野健一, 徳永千春, 原賢太, 米澤一仁

  第23回日本分子生物学会年会（神戸）, Dec. 2000, Japanese, 日本分子生物学会, 神戸国際展示場, Japan, Domestic conference

  Poster presentation


• ラパマイシン標的蛋白質mTORとPhosphoinositide dependent kinaseによるp70 S6 kinaseの活性化機構の解析

  原賢太, 大城紀子, 南太一, 吉野健一, 徳永千春, スユティ・ヒダヤット, 米澤一仁

  第73回日本生化学会大会（横浜）, Oct. 2000, Japanese, 日本生化学会, パシフィコ横浜, Japan, Domestic conference

  Poster presentation


• ラパマイシン標的蛋白質mTORキナーゼとPhospho-inositide dependent kinase (PDK1)によるp70 S6キナーゼ(p70)の活性化機構の解析

  原賢太, 吉野健一, 大城紀子, 徳永千春, スユティ・ヒダヤット, 米澤一仁

  第43回日本糖尿病学会年次学術集会（名古屋）, May 2000


• Site-directed mutagenesisを用いた抗Stx2マウスモノクローナル抗体のエピトープの解析

  中尾浩史, 林栄, 吉野健一, 内田寛, 山崎伸二, 竹田多惠

  第3回腸管出血性大腸菌感染症シンポジウム（東京）, Jul. 1998


• ヒトや種々の動物の血清中に検出される抗VT1及び抗VT2中和抗体の意義

  足立枝里子, 丸山務, 吉野健一, 竹田多惠

  第71回日本細菌学会総会（松本）, Apr. 1998, Japanese, 日本細菌学会, 長野県松本文化会館、松本市総合体育館, Japan, Domestic conference

  Poster presentation


• 1997年、当研究部での腸管出血性大腸菌O157患者の検出状況

  山形匡子, 内田寛, 吉野健一, 中尾浩史, 竹田多惠

  第72回日本感染症学会（大阪）, Apr. 1998


• VT2に対するマウスモノクローナル抗体のエピトープ解析

  吉川 和幸, 林 栄, 吉野 健一, 山崎 伸二, 木村 剛, 松本 洋一, 今泉 厚, 竹田 多恵

  第78回日本細菌学会関東支部総会, Oct. 1997, Japanese, 横浜ロイヤルパークホテルニッコー, Japan, Domestic conference

  Oral presentation


• 膜結合型グアニル酸シクラーゼのリン酸化部位

  鈴木範男, 古屋博隆, 野村晃司, 吉野健一, 竹田多恵

  第70回日本生化学会大会, Sep. 1997, Japanese


• 1996年全国アンケートによる大腸菌感染症O157の実態調査

  竹田 多恵, 吉野 健一, 内田 寛, 谷村 雅子, 池田 紀和

  第２回腸管出血性大腸菌感染症シンポジウム（堺）, Aug. 1997


• 腸管出血性大腸菌O157の産生するベロ毒素に対する吸着剤のin vitro薬効評価

  松田枝里子, 吉野健一, 竹田多恵

  第77回日本細菌学会関東支部総会, Jun. 1997, Japanese, アルカディア市ヶ谷, Domestic conference

  Nominated symposium


• 腸管出血性大腸菌O157:H7の非培養検査法の評価

  山形匡子, 松田枝里子, 吉野健一, 竹田多恵

  第77回日本細菌学会関東支部総会, Jun. 1997, Japanese, アルカディア市ヶ谷, Japan, Domestic conference

  Poster presentation


• Questionnaire-based Clinical Aspects of VTEC Infection in Japan, 1996

  Tae TAKEDA, Masako TANIMURA, Ken-ichi YOSHINO, Kyoko YAKAGATA, Eriko MATSUDA, Hiroshi UCHIDA, Norikazu IKEDA

  3rd International Symposium and Workshop on Shiga Toxin (Verocytotoxin)-Producing Escherrichia coli Infection (VTEC '97), Jun. 1997, English, Renaissane Harborplace Hotel, Baltimore, Maryland, United States, International conference

  Poster presentation


• Early use of antibiotics for STEC O157 infection rduces the risk of hemolytic uremic syndrome

  Tae TAKEDA, Masako TANIMURA, Ken-ichi YOSHINO, Eriko MATSUDA, Hiroshi UCHIDA, Norikazu IKEDA

  3rd International Symposium and Workshop on Siga Toxin (Verocytotoxin)-Producing Escherichia coli Infections (VTEC '97), Jun. 1997, English, Renaissane Harborplace Hotel, Baltimore, Maryland, United States, International conference

  Invited oral presentation


• Failure of immune response to Shiga toxin-producing Escherichia coli O157 lipopolysaccharide antigen in healthy carriers

  Ken-ichi YOSHINO, Eriko MATSUDA, Tae TAKEDA

  3rd International Symposium and Workshop on Shiga Toxin (Verocytotoxin)-Producing Escherrichia coli Infection (VTEC '97), Jun. 1997, English, Renaissane Harborplace Hotel, Baltimore, Maryland, United States, International conference

  Poster presentation


• Rapid Disappearance of Fecal Verotoxins in Patients with VTEC O157:H7 Infection who were Given Antibiotics at Early Stage of the Disease

  Eriko MATSUDA, Tae TAKEDA, Ken-ichi YOSHINO, Masashi SHIOMI, Kyoko YAMAGATA

  3rd International Symposium and Workshop on Shiga Toxin (Verocytotoxin)-Producing Escherrichia coli Infection (VTEC '97), Jun. 1997, English, Renaissane Harborplace Hotel, Baltimore, Maryland, United States, International conference

  Poster presentation


• Two Major Clones were Found during a Crisis of VTEC O 157:H7 (VT1+, VT2+) Infection in Japan, 1996

  Kyoko YAMAGATA, T. Ramamurthy, Amit PAL, Ken-ichi YOSHINO, Toshie KOYAMA, Hiromichi FUJISHIMA, Tae TAKEDA

  3rd International Symposium and Workshop on Shiga Toxin (Verocytotoxin)-Producing Escherrichia coli Infection (VTEC '97), Jun. 1997, English, Renaissane Harborplace Hotel, Baltimore, Maryland, United States, International conference

  Poster presentation


• Hemolytic Urine Syndrome

  Tae TAKEDA, Ken-ichi YOSHINO, Hiroshi UCHIDA, Kyoko YAMAGATA, Eriko MATSUDA, Norikazu IKEDA, Jun-ichiro FUJIMOTO

  The 6th China-Japan International Congress of Microbiology, Jun. 1997, English

  [Invited]

  Nominated symposium


• ベロ毒素産生性大腸菌O121: H19による溶血性尿毒症症候群の１幼児例

  鈴木与志晴, 大西雅子, 佐々木栄一, 吉野健一, 竹田多恵

  第32回日本小児腎臓病学会学術集会（大阪）, Jun. 1997


• 病原性大腸菌O165によると考えられた溶血性尿毒症症候群の２例

  西澤恭子, 長岡理恵子, 渡辺晴子, 木屋恵美, 大友義之, 松原知代, 小口学, 金子一成, 福田豊, 薮田 敬次郎, 竹田 多恵, 吉野健一

  第32回日本小児腎臓病学会学術集会（大阪）, Jun. 1997


• 腸管出血性大腸O157の迅速検出キットの評価

  松田 枝里子, 山形 匡子, 吉野 健一, 内田 寛, 竹田 多恵, 小川 道永

  第71回日本感染症学会総会, Apr. 1997, Japanese, ロイトン札幌（札幌市中央区北１条西１１丁目）, Japan, Domestic conference

  Oral presentation


• 1996年日本各地で分離された腸管出血性大腸菌O157の分子疫学

  池田 紀和, Ramamurthy T, Pal A, 吉野 健一, 竹田 多恵

  第70回日本細菌学会総会（宇都宮）, Mar. 1997, Japanese, 日本細菌学会, 栃木県総合文化センター, Japan, Domestic conference

  Nominated symposium


• ヒト血清中のベロ毒素２(VT2)中和因子

  木村 剛, 松本 洋一, 鈴木 洋二, 吉野 健一, 竹田 多恵

  第70回日本細菌学会総会（宇都宮）, Mar. 1997, Japanese, 日本細菌学会, 栃木県総合文化センター, Japan, Domestic conference

  Nominated symposium


• 腸管出血性大腸菌O157に対する乳酸菌、ビフィズス菌の感染予防効果

  小川 道永, 吉野 健一, 田中 隆一郎, 竹田 多恵

  第70回日本細菌学会総会（宇都宮）, Mar. 1997, Japanese, 日本細菌学会, 栃木県総合文化センター, Japan, Domestic conference

  Nominated symposium


• 一般健康成人からの大腸菌O157の分離頻度

  竹田 多恵, 吉野 健一

  第70回日本細菌学会総会（宇都宮）, Mar. 1997, Japanese, 日本細菌学会, 栃木県総合文化センター, Domestic conference

  Nominated symposium


• ベロ毒素産生性大腸菌O157LPSに対する血中抗体価にみられる小児と成人との差異

  吉野 健一, 松田 枝里子, 竹田 多恵

  第70回日本細菌学会総会（宇都宮）, Mar. 1997, Japanese, 日本細菌学会, 栃木県総合文化センター, Japan, Domestic conference

  Nominated symposium


• Hemolytic uremic syndrome associated with verotoxin-producing Escherichia coli

  Tee TAKEDA, Hiroshi UCHIDA, Ken-ichi YOSHINO, Thandavarayan Ramamuruthy, Amit PAL, Eriko MATSUDA, Jun-ichiro FUJIMOTO

  The 32nd Joint Conference of Cholera and Related Diarrheal Diseases Panel in U.S.-Japan Cooperative Medical Science Program, Nov. 1996, English, Nagasaki University Pompe Hall, Japan, International conference

  Oral presentation


• Identification of the functional regions of the superantigen Yersinia pseudotuberculosis-derived mitogen (YPM) by characterizing a novel variant molecule and by using the synthetic peptide fragments

  Ken-ichi Yoshino, Thandavarayan Ramamurthy, Tae Takeda

  The 32nd Joint Conference of Cholera and Related Diarrheal Diseases Panel in U.S.-Japan Cooperative Medical Science Program, Nov. 1996, English, Nagasaki University Pompe Hall, Japan, International conference

  Oral presentation


• 本年国内で集中発生したVTEC O157 のパルスフィールドゲル電気泳動法による分子疫学

  T. ラマムーティー, A. パル, 吉野 健一, 竹田 多恵

  第76回日本細菌学会関東支部総会, Nov. 1996, English, 東京女子医科大学弥生記念講堂（東京都新宿区河田町8-1）, Japan, Domestic conference

  Oral presentation


• 本年当院で分離したVTEC O157と岡山・邑久町での集団発生株との類似性

  竹田 多恵, T. ラマムーティー, 吉野 健一

  第76回日本細菌学会関東支部総会, Nov. 1996, Japanese, 東京女子医科大学弥生記念講堂（東京都新宿区河田町8-1）, Japan, Domestic conference

  Oral presentation


• VTEC O157感染症に対するグロブリン製剤の有用性

  内田 寛, 松田 枝里子, 吉野 健一, 竹田 多恵

  第76回日本細菌学会関東支部総会, Nov. 1996, Japanese, 東京女子医科大学弥生記念講堂（東京都新宿区河田町8-1）, Japan, Domestic conference

  Oral presentation


• 本年集中発生したHUSのVTEC O157 LPSに対する血中抗体の検出頻度

  松田 枝里子, 吉野 健一, 竹田 多恵

  第76回日本細菌学会関東支部総会, Nov. 1996, Japanese, 東京女子医科大学弥生記念講堂（東京都新宿区河田町8-1）, Japan, Domestic conference

  Oral presentation


• 便の増菌培養液を用いたPCRによるVTEC感染症の迅速診断

  吉野 健一, T. ラマムーティー, 松田 枝里子, 竹田 多恵

  第76回日本細菌学会関東支部総会, Nov. 1996, Japanese, 東京女子医科大学弥生記念講堂（東京都新宿区河田町8-1）, Japan, Domestic conference

  Oral presentation


• O157大腸菌による集団食中毒から２次感染した一例

  石井 祥子, 国富 泰二, 産賀 恵子, 内山 宙三, 三宅 陽子, 浜渦 庄司, T. ラマムーティー, 吉野 健一, 竹田 多恵

  第28回日本小児感染症学会, Nov. 1996, Japanese, 日本小児感染症学会, 砂防会館（東京都千代田区平河町2-7-5）, Japan, Domestic conference

  Oral presentation


• 当院でのベロ毒素産生性大腸菌O157によるHUSの散発例－患者の姉から原因菌が検出された意義－

  鈴木 輝明, 田川 学, 宇津木 忠仁, 吉野 健一, T. ラマムーティー, 松田 枝里子, 香坂 隆夫, 竹田 多恵

  第28回日本小児感染症学会, Nov. 1996, Japanese, 日本小児感染症学会, 砂防会館（東京都千代田区平河町2-7-5）, Japan, International conference

  Oral presentation


• パルスフィールドゲル電気泳動法によるベロ毒素産生性大腸菌（VTEC） O157の分子疫学

  T. ラマムーティー, A. パル, 松田 枝里子, 吉野 健一, 内田 寛, 金谷 誠久, 市場 洋三, 鈴木 輝明, 田川 学, 宇津木 忠仁, 岡本 則彦, 柏 弘, 河野 祥二, 佐橋 剛, 山口 久美子, 竹田 多恵

  第28回日本小児感染症学会, Nov. 1996, Japanese, 日本小児感染症学会, 砂防会館（東京都千代田区平河町2-7-5）, Japan, Domestic conference

  Oral presentation


• ベロ毒素産生性大腸菌（VTEC） O157検出のためのPCR法の有用性

  吉野 健一, T. ラマムーティー, 松田 枝里子, 内田 寛, 竹田 多恵

  第28回小児感染症学会, Nov. 1996, Japanese, 日本小児感染症学会, 砂防会館（東京都千代田区平河町2-7-5）, Domestic conference

  Oral presentation


• Incidence of Novel Subtype Heat-Stable Enterotoxin Genes Among Non-Epidemic Yersinia enterocolitica Strains

  Thandavarayan RAMAMURTHY, Ken-ichi YOSHINO, Tae TAKEDA

  The International Congress for Tripical Medicine and Malaria, Nov. 1996, English, Nagasaki University, Domestic conference


• スーパー抗原YPMのリンパ球増殖活性を特異的に阻害するペプチド

  吉野 健一, 高尾 敏文, 石橋 真紀子, 鮫島 勇次, 下西 康嗣, 竹田 多恵

  第43回毒素シンポジウム, Jul. 1996, Japanese, 加賀観光ホテル（加賀市）, Japan, Domestic conference

  Poster presentation


• スーパー抗原YPMのサブタイプ分子YPMbの精製、構造決定およびその性質

  竹田 多恵, T. Ramamurthy, 吉野 健一, 池田 紀和, 加藤 敬香

  第43回毒素シンポジウム, Jul. 1996, Japanese, 加賀観光ホテル（加賀市）, Japan, Domestic conference

  Oral presentation


• 腸管出血性大腸菌O157によるHUSの集中発生とその分子疫学

  竹田多恵, 吉野健一, T.ラマムーティー, 松田枝里子, 内田寛, 鈴木輝明, 宇津木忠仁, 産賀恵子, 金谷誠久, 市場洋三

  日本小児腎臓病学会第31回学術集会, Jul. 1996, Japanese, 前橋商工会議所会館, Domestic conference

  Oral presentation


• MALDI-TOF MS of Oligosaccharides Derivatized with 4-Aminobenzoic Acid 2-(Dimethylamino)ethyl Ester

  Toshifumi TAKAO, Yanet TAMBARA, Akihiro NAKAMURA, Ken-ichi YOSHINO, Yasutsugu SHIMONISHI

  44th ASMS Conference on Mass Spectrometry and Allied Topics, May 1996, English, American Society for Mass Spectrometry, Oregon Convetion Center, Portland, United States, International conference


• Yersinia enterocolitica産生するY-STbの遺伝子塩基配列および血清型O:5 Biotype 1Aとの高い相関

  T. Ramamurthy, 吉野 健一, 竹田 多恵

  第69回日本細菌学会総会（福岡）, Mar. 1996, English, 日本細菌学会, アクロス福岡, Japan, Domestic conference

  Poster presentation


• スーパー抗原YPMのサブタイプ分子YPMbの遺伝子クローニングと大腸菌での発現

  池田紀和, T.Ramamurthy, 吉野健一, 加藤敬香, 竹田多恵

  第69回日本細菌学会総会（福岡）, Mar. 1996, Japanese, 日本細菌学会, アクロス福岡, Domestic conference

  Poster presentation


• エルシニア菌の産生するスーパー抗原(YPM)の大量調製法の確立

  竹田 多恵, 吉野 健一

  第69回日本細菌学会総会（福岡）, Mar. 1996, Japanese, 日本細菌学会, アクロス福岡, Japan, Domestic conference

  Poster presentation


• スーパー抗原YPMのサブタイプ分子YPMbの精製とその性状

  石橋 真紀子, 吉野 健一, 鮫島 勇次, 竹田 多恵

  第69回日本細菌学会総会（福岡）, Mar. 1996, Japanese, 日本細菌学会, アクロス福岡, Japan, Domestic conference

  Poster presentation


• スーパー抗原YPMのマイトジェン活性を阻害するYPM部分ペプチドの調製

  吉野健一, 石橋真紀子, 鮫島勇次, 下西康嗣, 竹田多恵

  第69回日本細菌学会総会（福岡）, Mar. 1996, Japanese, 日本細菌学会, アクロス福岡, Japan, Domestic conference

  Poster presentation


• Purification and characterization of a novel superantigen produced by Yersinia pseudotuberculosis

  Ken-ichi Yoshino, Thandavarayan Ramamurthy, Jun Abe, Yasuhiko Ito, G. Balakrish Nair, Toshifumi Takao, Takao Kohsaka, Yasutsugu Shimonishi, Tae Takeda

  The 31st Joint Conference of Cholera and Related Diarrheal Diseases, Dec. 1995, English, The Unitted States-Japan Cooperative Medical Science Program, Kiawah Island, SC, USA, United States, International conference, Co-authored internationally

  Oral presentation


• Molecular epidemiology of Yersinia pseudotuberculosis by pulsed-field gel electrophoresis

  T. Ramamurthy, G. B. Nair, Hiroshi Fukushima, Seiji Kaneko, Ken-ichi Yoshino, Tae Takeda

  第74回日本細菌学会関東支部総会, Oct. 1995, English, 駒場エミナース（東京都目黒区大橋2-19-5）, Japan, Domestic conference, Co-authored internationally

  Oral presentation


• 耐熱性エンテロトキシン毒性ドメインのＮ末端側近傍に存在するアミノ酸残基の毒性に及ぼす役割

  吉野 健一, 黄 暁哲, 宮地 美貴, 高尾 敏文, 中尾 浩史, 下西 康嗣, 竹田 多恵

  第74回日本細菌学会関東支部総会, Oct. 1995, Japanese, 駒場エミナース（東京都目黒区大橋2-19-5）, Japan, Domestic conference

  Oral presentation


• 成熟毒素分子として“プロ領域”を含む耐熱性エンテロトキシンY-STcの前駆体蛋白質とリコンビナントY-STcの構造

  吉野 健一, 黄 暁哲, 中尾 浩史, 竹田 多恵

  第42回毒素シンポジウム, Aug. 1995, Japanese, 石橋文化センター（久留米市野中町1015）, Japan, Domestic conference

  Poster presentation


• 新規スーパー抗原Yersinia pseudotuberculosis-derived Mitogen (YPM)をコードする遺伝子の塩基配列およびその遺伝子頻度における地理的相違

  竹田 多恵, 吉野 健一, T. Ramamurthy, 伊藤 恭彦, 阿部 淳, 香坂 隆夫, 福島 博

  第42回毒素シンポジウム, Aug. 1995, Japanese, 石橋文化センター（久留米市野中町1015）, Japan, Domestic conference

  Oral presentation


• Sequence Analysis of the Gene for a Novel Superantigen Produced by Yersinia pseudotuberculosis and Characterization of the Recombinat Protein

  Yasuhiko ITO, Jun ABE, Ken-ichi YOSHINO, Tae TAKEDA, Takao KOHSAKA

  The 9th International Congress of Immunology, Jul. 1995, English, International conference


• Establishment of a novel superantigen produced by Yersinia pseudotuberculosis and its association with systemic Kawasaki disease-like symptoms

  Tae Takeda, Jun Abe, Ken-ichi Yoshino, Yasuhiko Itoh, Shin-ichi Matsumoto, Yasutsugu Shimonishi, Takao Kohsaka, Noboru Kobayashi

  5th International Kawasaki Disease Symposium (Fukuoka), May 1995, English, ACROS Fukuoka, International Convention Hall, Japan, International conference


• Genetic analysis of a novel superantigen produced by Yersinia pseudotuberculosis and its effect in acute phase patients

  Jun Abe, Yasuhiko Ito, Ken-ichi Yoshino, Shin-ichi Matsumoto, Tae Takeda, Takao Kohsaka

  Annual Meeting of Professional Research Scientists, Experimental Biology 95 (Atlanta, GA, USA), Apr. 1995, English


• ST毒素ファミリーのうち最大分子量を有するYersinia enterocolitica,/i>の新規なY-STサブタイプの遺伝子クローニングと大腸菌での発現

  黄暁哲, 吉野健一, 中尾浩史, 竹田多恵

  第68回日本細菌学会総会（京都府相楽郡）, Mar. 1995, Japanese, 日本細菌学会, けいはんな学研都市・けいはんなプラザ, Japan, Domestic conference

  Poster presentation


• Yersinia enterocoliticaの２種の新規なST毒素遺伝子の特異プローブの調製とその分布

  T. Ramamurthy, 黄暁哲, 丸山努, 大友良光, 吉野健一, 中尾浩史, 竹田多恵

  第68回日本細菌学会総会（京都府相楽郡）, Mar. 1995, English, 日本細菌学会, けいはんな学研都市・けいはんなプラザ, Japan, Domestic conference

  Poster presentation


• Yersinia pseudotuberculosis由来新規スーパー抗原をコードする遺伝子の分布

  吉野健一, T. Ramamurthy, 福島博, 大友良光, 竹田多恵

  第68回日本細菌学会総会（京都府相楽郡）, Mar. 1995, Japanese, 日本細菌学会, けいはんな学研都市・けいはんなプラザ, Japan, Domestic conference

  Poster presentation


• Yersinia pseudotuberculosisの産生するスーパー抗原の遺伝子解析と組換体蛋白の発現

  伊藤恭彦, 阿部淳, 吉野健一, 下西康嗣, 竹田多恵, 香坂隆夫

  第68回日本細菌学会総会（京都府相楽郡）, Mar. 1995, Japanese, 日本細菌学会, けいはんな学研都市・けいはんなプラザ, Japan, Domestic conference

  Poster presentation


• Amino acid sequences of newly recognized two subtypes of heat-stable enterotoxin produced by Yersinia enterocolitica

  Tae Takeda, Ken-ichi Yoshino, Hiroshi Nakao, Huang, XiaoZhe, Lai Xinhe, Miki Miyachi, Hiroshi Murata, Toshifumi Takao, Yasutsugu Shimonishi

  The 30th Joint Conference of Cholera and Related Diarrheal Diseases Panel, Dec. 1994, English, Cholera and Related Diarrheal Diseases Panel, The Unitted States-Japan Cooperative Medical Science Program, Kyushu University Alumnus Hall, Japan, International conference

  Oral presentation


• Yersinia pseudotuberculosisの産生するスーパー抗原の高度精製とその遺伝子のクローニング

  伊藤恭彦, 阿部淳, 吉野健一, 下西康嗣, 竹田多恵, 香坂隆夫

  第24回日本免疫学会総会・学術集会, Nov. 1994, Japanese, 日本免疫学会, 国立京都国際会館, Japan, Domestic conference


• Yersinia pseudotuberculosisの産生するスーパー抗原の高度精製とその遺伝子解析

  伊藤 恭彦, 阿部 淳, 吉野 健一, 下西 康嗣, 竹田 多恵, 香坂 隆夫

  第14回川崎病研究会（和歌山）, Oct. 1994, Japanese, 和歌山市民会館, Japan, Domestic conference


• 質量分析法による糖蛋白質の糖鎖構造解析

  吉野健一, 宮地美貴, 高尾敏文

  大阪大学蛋白質研究所セミナー 蛋白質の一次構造解析法の現状と展望, Oct. 1994, Japanese, 大阪大学蛋白質研究所, 大阪大学蛋白質研究所, Japan, International conference

  [Invited]

  Public discourse


• 精子活性化ペプチド受容体の構造

  清水健, 吉野健一, 鈴木範男

  日本動物学会第65回大会（名古屋）, Oct. 1994


• Purification and cloning of a gene encoding a novel superantigen produced by a clinical strain of Yersinia pseudotuberculosis

  Tae TAKEDA, Ken-ichi YOSHINO, Yasuhiko ITOH, Jun ABE, Yasutsugu SHIMONISHI, Takao KOHSAKA

  6th International Symposium on Yersinia (Rome, ITALY), Sep. 1994, English


• バフンウニ精子活性化ペプチド受容体の構造

  清水 健, 吉野 健一, 鈴木 範男

  平成6年度日本動物学会北海道支部大会（旭川）, Aug. 1994, Japanese


• Yersinia pseudotuberculosis由来スーパー抗原の高比活性精製標品の分離とその蛋白質化学的性質

  吉野健一, 阿部淳, 村田博, 高尾敏文, 香坂隆夫, 下西康嗣, 竹田多恵

  第41回毒素シンポジウム, Jul. 1994, Japanese, アルカディア市ヶ谷 私学会館（東京都千代田区九段北4-2-25）, Japan, Domestic conference

  Oral presentation


• Chemical Derivatization for High-Sensitive Detection of Oligosaccharides in Electrospray Ionization Mass Spectrometry

  Ken-ichi YOSHINO, Toshifumi TAKAO, Hiroshi MURATA, Yasutsugu SHIMONISHI

  The 42nd ASMS Conference on Mass Spectrometry and Allied Topics, Jun. 1994, English, American Society for Mass Spectrometry, The Hyatt Regency Hotel Chicago, IL, United States, International conference

  Poster presentation


• Yersinia pseudotuberculosisの産生するスーパー抗原精製法の検討

  吉野 健一, 阿部 淳, 村田 博, 高尾 敏文, 香坂 隆夫, 下西 康嗣, 竹田 多恵

  第67回日本細菌学会総会, Mar. 1994, Japanese, 日本都市センター（東京都新宿区河田町2-4-1）, Japan, Domestic conference

  Poster presentation


• 大腸菌内で合成した植物フェレドキシン分子の不均一性

  丸井洋二, 松村智裕, 角野富三郎, 長谷俊治, 吉野健一, 高尾敏文, 下西康嗣

  第66回日本生化学会大会（東京）, Oct. 1993, Japanese, 日本生化学会, 東京大学教養学部, Japan, Domestic conference

  Poster presentation


• エレクトロスプレーイオン化質量分析法による糖蛋白質糖鎖のミクロヘテロジェネイティの解析

  宮地美貴, 吉野健一, 村田博, 高尾敏文, 下西康嗣

  第66回日本生化学会大会（東京）, Oct. 1993, Japanese, 日本生化学会, 東京大学教養学部, Japan, Domestic conference

  Poster presentation


• エレクトロスプレーイオン化質量分析法によるオリゴ糖高感度分析：2-Diethylaminoethyl 4-aminobenzoate誘導体化

  吉野健一, 高尾敏文, 村田博, 下西康嗣

  第66回日本生化学会大会（東京）, Oct. 1993, Japanese, 日本生化学会, 東京大学教養学部, Japan, Domestic conference

  Oral presentation


• Yersinia pseudotuberculosisの産生するスーパー抗原の精製と性状

  竹田多恵, 阿部淳, 中尾浩史, 銭場俊彦, 吉野健一, 武田修明, 田中睦男, 下西康嗣, 香坂隆夫, 小林登

  第40回毒素シンポジウム, Jul. 1993, Japanese, 花びしホテル（函館市湯川町1-16-18）, Domestic conference

  Oral presentation


• Yersinia enterocolitica 84-50株および86-11株の産生する２種類の新しい耐熱性エンテロトキシンの単離、構造決定

  吉野 健一, 黄暁哲, 宮地美貴, 高尾敏文, 村田博, 中尾浩史, 頼心河, 竹田多恵, 下西康嗣

  第40回毒素シンポジウム, Jul. 1993, Japanese, 花びしホテル（函館市湯川町1-16-18）, Japan, Domestic conference

  Oral presentation


• Yersinia enterocoliticaの産生する新しい耐熱性エンテロトキシンの性状と一次構造

  黄 暁哲, 吉野 健一, 中尾 浩史, 春日 秀仁, 袁 佩娜, 小川 哲, 宮地 美貴, 高尾 敏文, 下西 康嗣, 竹田 多恵

  第66回日本細菌学会総会, Mar. 1993, Japanese, 名古屋国際会議場（名古屋市熱田区熱田西町１番１号）, Japan, Domestic conference

  Poster presentation


• Vibrio cholerae O1の産生する耐熱性エンテロトキシンの一次構造

  吉野 健一, 宮地 美貴, 高尾 敏文, P. K. Bag, 黄 暁哲, G. B. Nair, 竹田 多恵, 下西 康嗣

  第66回日本細菌学会総会, Mar. 1993, Japanese, 名古屋国際会議場（名古屋市熱田区熱田西町１番１号）, Japan, Domestic conference, Co-authored internationally

  Poster presentation


• 新しく単離されたシスチン含有精子活性化ペプチドのタンデム質量分析法による一次構造解析

  吉野 健一, 高尾 敏文, 下西 康嗣, 鈴木 範男

  第29回ペプチド化学討論会, Nov. 1991, Japanese, 日本ペプチド学会, 共立講堂（東京都千代田区神田一ツ橋2-2-1 共立女子大学）, Japan, Domestic conference

  Poster presentation


• Identification and characterization of specific receptors for sperm-activating peptide III (SAP-III)

  Ken-ichi YOSHINO, Norio SUZUKI, Toshifumi TAKAO, Yasutsugu SHIMONISHI

  日本動物学会第62回大会（岡山）, Oct. 1991, Japanese, 日本動物学会, 岡山大学教養部, Japan, Domestic conference

  Oral presentation


• The presence of o-, m-, and p-bromophenylalanines and bromohistidine in sperm-activating peptides

  Ken-ichi YOSHINO, Toshifumi TAKAO, Masahiko SUHARA, Yasutsugu SHIMONISHI, Norio SUZUKI

  1st Biennial International Conference on Amino Acid Research, Aug. 1991, English, Kyoto International Conference Hall, Japan, International conference

  Poster presentation


• 受精における精子活性化機構：卵ジェリーに含まれる精子活性化ペプチドとα-アミノイソ酪酸 (Aib) の効果

  吉野健一

  第24回若手ペプチド夏の勉強会（東浦町・淡路島）, Aug. 1991, Japanese, 東浦サンパーク（津名郡東浦町、淡路島）, Japan, Domestic conference

  Oral presentation


• Primary Structures of Bromophenylalanine-containing Sperm-Activating Peptides

  Ken-ichi YOSHINO, Toshifumi TAKAO, Hideaki HORI, Masahiro SUHARA, Tetsuya KITAI, Kohji NOMURA, Masayuki YAKAGUCH, Yasutsugu SHIMONISHI, Norio SUZUKI

  The 28th Symposium on Peptide Chemistry, Oct. 1990, Japanese, 日本ペプチド学会, 千里協栄生命ホール（大阪府豊中市千里西1-1-10）, Japan, Domestic conference

  Oral presentation


• Identification of a novel amino acid, L-ortho-bromopehylalanine, in sperm-activating peptides from the egg jelly of the sea urchin Tripneustes gratilla

  吉野健一, 山口正晃, 鈴木範男, 高尾敏文, 下西康嗣, 須原正彦, 北井哲也

  日本動物学会第61回大会, Oct. 1990, Japanese, 日本動物学会, 新潟大学工学部, Domestic conference

  Oral presentation


• Two novel brominated amino acids-containig sperm activating peptides isolated from the egg jelly of sea urchins, Tripneustes gratilla and Clypeaster japonicus

  Ken-ichi YOSHINO, Toshifumi TAKAO, Kohji NOMURA, Masaaki Yamaguchi, Yasutsugu SHIMONISHI, Norio SUZUKI

  7th International Echinoderm Conference, Sep. 1990, English, Hotel Chateutel Akanezaki, Atami, Japan, International conference

  Poster presentation


• 精子活性化ペプチドⅠ（SAP-I）の構造活性相関

  吉野健一

  第22回若手ペプチド夏の勉強会（高遠町・長野県）, Jul. 1989, Japanese, Japan, Domestic conference

  Oral presentation


• Variations in the structures of sperm activating peptides from the egg jelly of sea urchins in order Echinoida

  吉野健一, 山口正晃, 鈴木範男, 野村晃司, 梶浦弘子

  59th Annual Meetings of the Zoological Society of Japan, Oct. 1988, Japanese, the Zoological Society of Japan, 札幌医科大学, Domestic conference

  Oral presentation


• Sperm Activating Peptide (SAP) の単離・精製・構造決定およびその生理作用

  吉野健一

  第21回若手ペプチド夏の勉強会（阿南市・徳島県）, Aug. 1988, Japanese, Japan

  Oral presentation


• Specificity of Sperm Activating Peptides

  Norio SUZUKI, Masaaki YAMAGUCHI, Ken-ichi YOSHINO, Masanori KURITA, Hiroshi TANAKA, Koji NOMURA, Hiroko KAJIURA

  The 58th Annual Meetings of the Zoological Society of Japan, Oct. 1987, Japanese, 日本動物学会, 富山大学, Japan, Domestic conference

  Oral presentation


• Purification and Structure of Sperm-Activating Peptides from the Egg Jelly of the Sea Urchin Diadema setosum

  Ken-ichi YOSHINO, Masaaki YAMAGUCHI, Norio SUZUKI, Koji NOMURA, Hiroko KAJIURA

  The 58th Annual Meeting of the Zoological Society of Japan, Oct. 1987, Japanese, the Zoological Society of Japan, 富山大学, Japan, Domestic conference

  Oral presentation


• Taxonomical significance of respiratory stimulation of sea urchin spermatozoa by egg associated substances

  Norio Suzuki, Ken-ichi Yoshino, Masanori Kurita, Masaaki Yamaguchi, Shonan Amemiya

  6th International Echinoderm Conference (Victoria, CANADA), Aug. 1987, English

  Oral presentation


• Speract結合量の測定、および結合部位の局在性

  栗田正徳, 吉野健一, 山口正晃, 鈴木範男

  昭和61年度日本動物学会中部支部秋季例会, Nov. 1986, Japanese, 日本動物学会中部支部, 富山市, Japan, Domestic conference

  Oral presentation


• Alloresact（アロレスアクト）誘導体の分離、精製および構造

  吉野健一, 栗田正徳, 梶浦弘子, 野村晃司, 山口正晃, 鈴木範男

  昭和61年度日本動物学会中部支部秋季例会, Nov. 1986, Japanese, 日本動物学会中部支部, 富山市, Domestic conference

  Oral presentation


• The effect of speract on the mobility of a high molecular weight protein of the sea urchin spermatozoa on SDS-polyacrylamide gels

  栗田正徳, 吉野健一, 山口正晃, 鈴木範男, 野村晃司

  日本動物学会第57回大会, Oct. 1986, Japanese, 日本動物学会, 九州大学箱崎法文系キャンパス, Japan, Domestic conference

  Oral presentation


• Isolation of sperm activating peptides from egg jelly of the sea urchin, Glyptocidaris crenularis

  吉野 健一, 栗田 正徳, 山口 正晃, 鈴木 範男, 梶浦 弘子

  日本動物学会第57回大会, Oct. 1986, Japanese, 日本動物学会, Fukuoka, Japan, Domestic conference

  Oral presentation


• Sperm-activating peptides from the egg jelly of various species of sea urchin.

  Norio SUZUKI, David L. GARBERS, Kohji NOMURA, Hiroko KAJIURA, Ken-ichi YOSHINO, Masanori KURITA, Masaaki YAMAGUCHI

  The 5th International Symposium on Spermatology, Aug. 1986, English, Fujiyoshida City, YAMANASHI, Japan, International conference, Co-authored internationally

  Oral presentation


• ホンウニ類卵ジェリー中の精子呼吸促進ペプチド

  鈴木 範男, 吉野 健一, 山口 正晃

  昭和61年度日本動物学会中部支部春季例会（津）, May 1986, Japanese

  Oral presentation