Research activity information

• Oct. 2024 令和6年度学長表彰（財務貢献者）, 神戸大学


• Oct. 2022 神戸大学工学部 令和4年度優秀教育賞（応用化学科）


• Oct. 2022 神戸大学, 令和4年度学長表彰（財務貢献者）


• Oct. 2021 神戸大学, 令和3年度学長表彰（財務貢献者）


• Oct. 2020 神戸大学, 令和2年度学長表彰（財務貢献者）


• Oct. 2019 神戸大学工学部, 平成30年度優秀教育賞（応用化学科）

  田中 勉


• Dec. 2014 酵素工学研究会, 酵素工学奨励賞, 微生物の細胞表層における酵素工学に関する研究

  TANAKA TSUTOMU

  Japan society


• Jan. 2014 日本農芸化学会, B.B.B.論文賞, Utilization of Lactic Acid Bacterial Genes in Synechocystis sp. PCC 6803 in the Production of Lactic Acid

  Ancy Joseph, Simpei Aikawa, Kengo Sasaki, Yota Tsuge, Fumio Matsuda, Tsutomu Tanaka, Akihiko Kondo

  Japan society


• Sep. 2013 日本生物工学会, 生物工学論文賞, Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  SOMA YUKI, INOKUMA KENTARO, TANAKA TSUTOMU, OGINO CHIAKI, KONDO AKIHIKO, OKAMOTO MASAHIRO, HANAI TAIZO

  Japan society


• Mar. 2011 化学工学会, 研究奨励賞, 酵素を用いたタンパク質高機能化技術の開発

  TANAKA Tsutomu

• Metabolic engineering of Escherichia coli for 4-nitrophenylalanine production via the 4-aminophenylalanine synthetic pathway

  Ayana Mori, Yuuki Hirata, Mayumi Kishida, Daisuke Nonaka, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

  Sep. 2025, Metabolic Engineering

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic Engineering for Resveratrol Production Based on Modularization of Metabolic Pathways in Escherichia coli

  Daisuke Nonaka, Mayumi Kishida, Yuuki Hirata, Ayana Mori, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

  May 2025, Journal of Agricultural and Food Chemistry

  Scientific journal


• Modular pathway engineering for enhanced production of para -aminobenzoic acid and 4-amino-phenylalanine in Escherichia coli via glucose/xylose co-utilization

  Daisuke Nonaka, Mayumi Kishida, Yuuki Hirata, Ayana Mori, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

  ABSTRACT The modularization of biosynthetic pathways is a promising approach for enhancing microbial chemical production. We have developed a co-utilization method with glucose and xylose substrates to divide metabolic pathways into distinct production and energy modules to enhance the biosynthesis of para -aminobenzoic acid (pABA) in Escherichia coli . Optimizing initial glucose/xylose concentrations and eliminating carbon leakage resulted in a pABA titer of 8.22 g/L (yield: 0.23 g/g glucose). This strategy was then applied to the biosynthesis of 4APhe, a compound synthesized from chorismate without pyruvate (PYR) release. Utilizing glucose and xylose as co-substrates resulted in the production of 4.90 g/L 4APhe. Although 4APhe production did not benefit from PYR-driven energy generation as pABA production did, high titer was still achieved. This study highlights the effectiveness of modular metabolic pathway division for enhancing the production of key aromatic compounds and provides valuable insight into microbial production of chemicals that require specific biosynthetic donors such as amino groups. IMPORTANCE Microbial biosynthesis of chemicals from renewable resources offers a sustainable alternative to fossil fuel-based production. However, inefficiencies due to substrate diversion into by-products and biomass hinder optimal yields. In this study, we employed a modular metabolic engineering approach, decoupling pathways for chemical production from cell growth. Using glucose and xylose as co-substrates, we achieved the enhancement of p -aminobenzoic acid production in Escherichia coli . Additionally, we demonstrated the versatility of this approach by applying it to the biosynthesis of 4-amino-phenylalanine production. This study highlights the potential of modular metabolic pathway division for increased production of target compounds and provides valuable insight into microbial production of chemicals that require specific biosynthetic donors such as amino groups.

  American Society for Microbiology, Apr. 2025, Applied and Environmental Microbiology

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Parallel metabolic pathway engineering for aerobic 1,2-propanediol production in Escherichia coli.

  Daisuke Nonaka, Yuuki Hirata, Mayumi Kishida, Ayana Mori, Ryosuke Fujiwara, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

  The demand for the essential commodity chemical 1,2-propanediol (1,2-PDO) is on the rise, as its microbial production has emerged as a promising method for a sustainable chemical supply. However, the reliance of 1,2-PDO production in Escherichia coli on anaerobic conditions, as enhancing cell growth to augment precursor availability remains a substantial challenge. This study presents glucose-based aerobic production of 1,2-PDO, with xylose utilization facilitating cell growth. An engineered strain was constructed capable of exclusively producing 1,2-PDO from glucose while utilizing xylose to support cell growth. This was accomplished by deleting the gloA, eno, eda, sdaA, sdaB, and tdcG genes for 1,2-PDO production from glucose and introducing the Weimberg pathway for cell growth using xylose. Enhanced 1,2-PDO production was achieved via yagF overexpression and disruption of the ghrA gene involved in the 1,2-PDO-competing pathway. The resultant strain, PD72, produced 2.48 ± 0.15 g L-1 1,2-PDO with a 0.27 ± 0.02 g g-1-glucose yield after 72 h cultivation. Overall, this study demonstrates aerobic 1,2-PDO synthesis through the isolation of the 1,2-PDO synthetic pathway from the tricarboxylic acid cycle.

  Aug. 2024, Biotechnology journal, 19(8) (8), e2400210, English, International magazine

  Scientific journal


• Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica.

  Ayumi Koshiba, Mariko Nakano, Yuuki Hirata, Rie Konishi, Yuta Matsuoka, Yuta Miwa, Ayana Mori, Akihiko Kondo, Tsutomu Tanaka

  Short-chain esters, particularly isobutyl acetate and isoamyl acetate, hold significant industrial value due to their wide-ranging applications in flavors, fragrances, solvents, and biofuels. In this study, we demonstrated the biosynthesis of acetate esters using Yarrowia lipolytica as a host by feeding alcohols to the yeast culture. Initially, we screened for optimal alcohol acyltransferases for ester biosynthesis in Y. lipolytica. Strains of Y. lipolytica expressing atf1 from Saccharomyces cerevisiae, produced 251 or 613 mg/L of isobutyl acetate or of isoamyl acetate, respectively. We found that introducing additional copies of ATF1 enhanced ester production. Furthermore, by increasing the supply of acetyl-CoA and refining the culture conditions, we achieved high production of isoamyl acetate, reaching titers of 3404 mg/L. We expanded our study to include the synthesis of a range of acetate esters, facilitated by enriching the culture medium with various alcohols. This study underscores the versatility and potential of Y. lipolytica in the industrial production of acetate esters.

  Jul. 2024, Biotechnology progress, e3499, English, International magazine

  Scientific journal


• p-Nitrobenzoate production from glucose by utilizing p-aminobenzoate N-oxygenase: AurF.

  Ayana Mori, Yuuki Hirata, Mayumi Kishida, Yutaro Mori, Akihiko Kondo, Shuhei Noda, Tsutomu Tanaka

  Nitroaromatic compounds are widely used in industry, but their production is associated with issues such as the hazardousness of the process and low regioselectivity. Here, we successfully demonstrated the production of p-nitrobenzoate (PNBA) from glucose by constructing p-aminobenzoate N-oxygenase AurF-expressing E. coli. We generated this strain, which we named PN-1 by disrupting four genes involved in PNBA degradation: nfsA, nfsB, nemA, and azoR. We then expressed AurF from Streptomyces thioluteus in this strain, which resulted in the production of 945 mg/L PNBA in the presence of 1 g/L p-aminobenzoate. Direct production of PNBA from glucose was achieved by co-expressing the pabA, pabB, and pabC, as well as aurF, resulting in the production of 393 mg/L PNBA from 20 g/L glucose. To improve the PNBA titer, we disrupted genes involved in competing pathways: pheA, tyrA, trpE, pykA, and pykF. The resultant strain PN-4Ap produced 975 mg/L PNBA after 72 h of cultivation. These results highlight the potential of using microorganisms to produce other nitroaromatic compounds.

  Dec. 2023, Enzyme and microbial technology, 171, 110321 - 110321, English, International magazine

  Scientific journal


• Triacetic acid lactone production using 2-pyrone synthase expressing Yarrowia lipolytica via targeted gene deletion.

  Yuta Matsuoka, Naofumi Fujie, Mariko Nakano, Ayumi Koshiba, Akihiko Kondo, Tsutomu Tanaka

  An environmentally sustainable world can be realized by using microorganisms to produce value-added materials from renewable biomass. Triacetic acid lactone (TAL) is a high-value-added compound that is used as a precursor of various organic compounds such as food additives and pharmaceuticals. In this study, we used metabolic engineering to produce TAL from glucose using an oleaginous yeast Yarrowia lipolytica. We first introduced TAL-producing gene 2-pyrone synthase into Y. lipolytica, which enabled TAL production. Next, we increased TAL production by engineering acetyl-CoA and malonyl-CoA biosynthesis pathways by redirecting carbon flux to glycolysis. Finally, we optimized the carbon and nitrogen ratios in the medium, culminating in the production of 4078 mg/L TAL. The strategy presented in this study had the potential to improve the titer and yield of polyketide biosynthesis.

  Oct. 2023, Journal of bioscience and bioengineering, 136(4) (4), 320 - 326, English, Domestic magazine

  Scientific journal


• Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum

  Misa Doke, Mayumi Kishida, Yuuki Hirata, Mariko Nakano, Mayo Horita, Daisuke Nonaka, Yutaro Mori, Ryosuke Fujiwara, Akihiko Kondo, Shuhei Noda, Tsutomu Tanaka

  Sciscan Publishing Limited, Jul. 2023, Synthetic Biology and Engineering, 1(2) (2), 10012 - 9, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Caffeic acid production from glucose using metabolically engineered Escherichia coli

  Kosuke Sakae, Daisuke Nonaka, Mayumi Kishida, Yuuki Hirata, Ryosuke Fujiwara, Akihiko Kondo, Shuhei Noda, Tsutomu Tanaka

  Elsevier BV, Mar. 2023, Enzyme and Microbial Technology, 164, 110193 - 110193, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of Schizosaccharomyces pombe for itaconic acid production.

  Fujie N, Ito M, Kishida M, Hirata Y, Akihiko Kondo, Tanaka T

  The economical production of value-added chemicals from renewable biomass is a promising aspect of producing a sustainable economy. Itaconic acid (IA) is a high value-added compound that is expected to be an alternative to petroleum-based chemicals. In this study, we developed a metabolic engineering strategy for the large-scale production of IA from glucose using the fission yeast Schizosaccharomyces pombe. Heterologous expression of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus, which encodes cis-aconitate decarboxylase in the cytosol, led to the production of 0.132 g/L of IA. We demonstrated that mitochondrial localization of CAD enhanced the production of IA. To prevent the leakage of carbon flux from the TCA cycle, we generated a strain in which the endogenous malate exporter, citrate lyase, and citrate transporter genes were disrupted. A titer of 1.110 g/L of IA was obtained from a culture of this strain started with 50 g/L of glucose. By culturing the multiple mutant strain at increased cell density, we succeeded in enhancing the IA production to 1.555 g/L. The metabolic engineering strategies presented in this study have the potential to improve the titer of the biosynthesis of derivatives of intermediates of the TCA cycle.

  Elsevier BV, Sep. 2022, Journal of biotechnology, 358, 111 - 117, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• G6P-capturing molecules in the periplasm of Escherichia coli accelerate the shikimate pathway

  Ryosuke Fujiwara, Mariko Nakano, Yuuki Hirata, Chisako Otomo, Daisuke Nonaka, Sakiya Kawada, Hikaru Nakazawa, Mitsuo Umetsu, Tomokazu Shirai, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Escherichia coli, the most studied prokaryote, is an excellent host for producing valuable chemicals from renewable resources as it is easy to manipulate genetically. Since the periplasmic environment can be easily controlled externally, elucidating how the localization of specific proteins or small molecules in the periplasm affects metabolism may lead to bioproduction development using E. coli. We investigated metabolic changes and its mechanisms occurring when specific proteins are localized to the E. coli periplasm. We found that the periplasmic localization of β-glucosidase promoted the shikimate pathway involved in the synthesis of aromatic chemicals. The periplasmic localization of other proteins with an affinity for glucose-6-phosphate (G6P), such as inactivated mutants of Pgi, Zwf, and PhoA, similarly accelerated the shikimate pathway. Our results indicate that G6P is transported from the cytoplasm to the periplasm by the glucose transporter protein EIICBGlc, and then captured by β-glucosidase.

  Elsevier BV, Jul. 2022, Metabolic Engineering, 72, 68 - 81, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• L‐Lactate oxidase‐mediated removal of L‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid

  Kenji Okano, Yu Sato, Shnji Hama, Tsutomu Tanaka, Hideo Noda, Akihiko Kondo, Kohsuke Honda

  BACKGROUND: There has been an increasing demand for optically pure d-lactic and l-lactic acid for the production of stereocomplex-type polylactic acid. The d-lactic acid production from lignocellulosic biomass is important owing to its great abundance in nature. Corn steep liquor (CSL) is a cheap nitrogen source used for industrial fermentation, though it contains a significant amount of l-lactic acid, which decreases the optical purity of d-lactic acid produced. METHOD AND RESULTS: To remove l-lactic acid derived from the CSL-based medium, l-lactate oxidase (LoxL) from Enterococcus sp. NBRC 3427 was expressed in an engineered Lactiplantibacillus plantarum (formally called Lactobacillus plantarum) strain KOLP7, which exclusively produces d-lactic acid from both hexose and pentose sugars. When the resulting strain was applied for d-lactic acid fermentation from the mixed sugars consisting of the major constituent sugars of lignocellulose (35 g L-1 glucose, 10 g L-1 xylose, and 5 g L-1 arabinose) using the medium containing 10 g L-1 CSL, it completely removed l-lactic acid derived from CSL (0.52 g L-1 ) and produced 41.7 g L-1 of d-lactic acid. The l-lactic acid concentration was below the detection limit, and improvement in the optical purity of d-lactic acid was observed (from 98.2% to > 99.99%) by the overexpression of LoxL. CONCLUSION AND IMPLICATIONS: The LoxL-mediated consumption of l-lactic acid would enable the production of optically pure d-lactic acid in any medium contaminated by l-lactic acid.

  Wiley, Apr. 2022, Biotechnology Journal, 17(4) (4), 2100331 - 2100331, English, International magazine

  [Refereed]

  Scientific journal


• Reprogramming Escherichia coli pyruvate-forming reaction towards chorismate derivatives production

  Shuhei Noda, Yutaro Mori, Ryosuke Fujiwara, Tomokazu Shirai, Tsutomu Tanaka, Akihiko Kondo

  Microbial metabolic pathway engineering is a potent strategy used worldwide to produce aromatic compounds. We drastically rewired the primary metabolic pathway of Escherichia coli to produce aromatics and their derivatives. The metabolic pathway of E. coli was compartmentalized into the production and energy modules. We focused on the pyruvate-forming reaction in the biosynthesis pathway of some compounds as the reaction connecting those modules. E. coli strains were engineered to show no growth unless pyruvate was synthesized along with the compounds of interest production. Production of salicylate and maleate was demonstrated to confirm our strategy's versatility. In maleate production, the production, yield against the theoretical yield, and production rate reached 12.0 g L-1, 67%, and up to fourfold compared to that in previous reports, respectively; these are the highest values of maleate production in microbes to our knowledge. The results reveal that our strategy strongly promotes the production of aromatics and their derivatives.

  Elsevier BV, Sep. 2021, Metabolic Engineering, 67, 1 - 10, English, International magazine

  [Refereed]

  Scientific journal


• Outcomes of Endoscopic Ultrasound-Guided Biliary Drainage in Patients Undergoing Antithrombotic Therapy

  Nozomi Okuno, Kazuo Hara, Nobumasa Mizuno, Shin Haba, Takamichi Kuwahara, Hiroki Koda, Masahiro Tajika, Tsutomu Tanaka, Sachiyo Onishi, Keisaku Yamada, Akira Miyano, Daiki Fumihara, Moaz Elshair

  Jul. 2021, Clinical Endoscopy

  Scientific journal


• Metabolic engineering of 1,2-propanediol production from cellobiose using beta-glucosidase-expressing E. coli

  Daisuke Nonaka, Ryosuke Fujiwara, Yuuki Hirata, Tsutomu Tanaka, Akihiko Kondo

  Elsevier Ltd, Jun. 2021, Bioresource Technology, 329, English

  Scientific journal


• Preparation of affinity membranes using polymer phase separation and azido-containing surfactants

  Kenta Morita, Shinano Takeda, Ayumi Yunoki, Takane Tsuchii, Tsutomu Tanaka, Tatsuo Maruyama

  Feb. 2021, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 611, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of shikimic acid-producing Corynebacterium glutamicum from glucose and cellobiose retaining its phosphotransferase system function and pyruvate kinase activities

  Naoki Sato, Mayumi Kishida, Mariko Nakano, Yuuki Hirata, Tsutomu Tanaka

  Sep. 2020, Frontiers in Bioengineering and Biotechnology, 8, 569406, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl‐CoA supply

  Daichi Satowa, Ryosuke Fujiwara, Shogo Uchio, Mariko Nakano, Chisako Otomo, Yuuki Hirata, Takuya Matsumoto, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Microbial production of mevalonate from renewable feedstock is a promising and sustainable approach for the production of value-added chemicals. We describe the metabolic engineering of Escherichia coli to enhance mevalonate production from glucose and cellobiose. First, the mevalonate-producing pathway was introduced into E. coli and the expression of the gene atoB, which encodes the gene for acetoacetyl-CoA synthetase, was increased. Then, the deletion of the pgi gene, which encodes phosphoglucose isomerase, increased the NADPH/NADP+ ratio in the cells but did not improve mevalonate production. Alternatively, to reduce flux toward the tricarboxylic acid cycle, gltA, which encodes citrate synthetase, was disrupted. The resultant strain, MGΔgltA-MV, increased levels of intracellular acetyl-CoA up to sevenfold higher than the wild-type strain. This strain produced 8.0 g/L of mevalonate from 20 g/L of glucose. We also engineered the sugar supply by displaying β-glucosidase (BGL) on the cell surface. When cellobiose was used as carbon source, the strain lacking gnd displaying BGL efficiently consumed cellobiose and produced mevalonate at 5.7 g/L. The yield of mevalonate was 0.25 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose). These results demonstrate the feasibility of producing mevalonate from cellobiose or cellooligosaccharides using an engineered E. coli strain.

  Wiley, Jul. 2020, Biotechnology and Bioengineering, 117(7) (7), 2153 - 2164, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose–xylose co-substrate

  Ryosuke Fujiwara, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2020, Nature Communications, 11, 279, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli.

  Takuya Matsumoto, Yuki Mori, Tsutomu Tanaka, Akihiko Kondo

  Bioamination methods using microorganisms have attracted much attention because of the increasing demand for environmentally friendly bioprocesses. n-Butylamine production from glucose in Escherichia coli was demonstrated in this study, which has never been reported because of the absence of n-butylamine-producing pathway in nature. We focused on a transaminase-mediated cascade for bioamination from an alcohol or aldehyde. The cascade can convert an alcohol or an aldehyde to the corresponding amine with l-alanine as an amine donor. Here, n-butyraldehyde, which is a metabolic intermediate in the n-butanol producing pathway, is a potential intermediate for producing n-butylamine using this cascade. Hence, the n-butanol-producing pathway and the transaminase-mediated cascade were combined into a synthetic metabolic pathway for producing n-butylamine from glucose. Firstly, we demonstrated the conversion of n-butanol to n-butylamine using a three enzyme-mediated cascade. n-Butanol was successfully converted to n-butylamine in 92% yield in the presence of l-alanine and ammonium chloride. Then, the n-butanol-producing pathway and transaminase-mediated cascade were introduced into E. coli. Using this system, n-butylamine was successfully produced from glucose as a carbon source at a concentration of 53.2 mg L-1 after 96 h cultivation using a ppc (phosphoenolpyruvate carboxylase)-deficient strain. To the best of our knowledge, this is the first report of the direct production of n-butylamine from glucose, and may provide a starting point for the development of microbial methods to produce other bioamines.

  Jan. 2020, Journal of bioscience and bioengineering, 129(1) (1), 99 - 103, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering to improve 1,5‐diaminopentane production from cellobiose using β‐glucosidase‐secreting Corynebacterium glutamicum

  Rena Matsuura, Mayumi Kishida, Rie Konishi, Yuuki Hirata, Noriko Adachi, Shota Segawa, Kenta Imao, Tsutomu Tanaka, Akihiko Kondo

  Oct. 2019, Biotechnology and Bioengineering, 116(10) (10), 2640 - 2651

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli

  Matsumoto, T, Mori, Y, Tanaka, T, Kondo, A

  2019, Journal of Bioscience and Bioengineering

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe

  TAKAYAMA Seiya, OZAKI Aiko, KONISHI Rie, OTOMO Chisako, KISHIDA Mayumi, HIRATA Yuuki, MATSUMOTO Takuya, TANAKA Tsutomu, KONDO Akihiko

  BACKGROUND: Economical production of value-added chemicals from renewable biomass is a promising path to sustainability. 3-Hydroxypropionic acid (3-HP) is an important chemical for building a bio-sustainable society. Establishment of 3-HP production from renewable resources such as glucose would provide a bio-sustainable alternative to the production of acrylic acid from fossil resources. RESULTS: Here, we describe metabolic engineering of the fission yeast Schizosaccharomyces pombe to enhance 3-HP production from glucose and cellobiose via the malonyl-CoA pathway. The mcr gene, encoding the malonyl-CoA reductase of Chloroflexus aurantiacus, was dissected into two functionally distinct fragments, and the activities of the encoded protein were balanced. To increase the cellular supply of malonyl-CoA and acetyl-CoA, we introduced genes encoding endogenous aldehyde dehydrogenase, acetyl-CoA synthase from Salmonella enterica, and endogenous pantothenate kinase. The resulting strain produced 3-HP at 1.0 g/L from a culture starting at a glucose concentration of 50 g/L. We also engineered the sugar supply by displaying beta-glucosidase (BGL) on the yeast cell surface. When grown on 50 g/L cellobiose, the beta-glucosidase-displaying strain consumed cellobiose efficiently and produced 3-HP at 3.5 g/L. Under fed-batch conditions starting from cellobiose, this strain produced 3-HP at up to 11.4 g/L, corresponding to a yield of 11.2% (g-3-HP/g-glucose; given that 1 g cellobiose corresponds to 1.1 g glucose upon digestion). CONCLUSIONS: In this study, we constructed a series of S. pombe strains that produced 3-HP via the malonyl-CoA pathway. Our study also demonstrated that BGL display using cellobiose and/or cello-oligosaccharides as a carbon source has the potential to improve the titer and yield of malonyl-CoA- and acetyl-CoA-derived compounds.

  Nov. 2018, Microbial Cell Factories, 17(1) (1), 176 - 176, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Muconic Acid Production Using Gene-Level Fusion Proteins in Escherichia coli

  Fujiwara, Ryosuke, Noda, Shuhei, Tanaka, Tsutomu, Kondo, Akihiko

  Oct. 2018, Acs Synthetic Biology, 7(11) (11)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 分裂酵母を用いた有機酸生産技術の開発

  TANAKA TSUTOMU, KONDO AKIHIKO

  化学工業社, Jul. 2018, ケミカルエンジニヤリング, 特集=革新技術が拓く新しい可能性, 63(7) (7), 7 - 10, Japanese

  Scientific journal


• Metabolic Engineering of Lactobacillus plantarum for Direct l-Lactic Acid Production From Raw Corn Starch

  Kenji Okano, Gentaro Uematsu, Shinji Hama, Tsutomu Tanaka, Hideo Noda, Akihiko Kondo, Kohsuke Honda

  Wiley-VCH Verlag, May 2018, Biotechnology Journal, 13(5) (5), e1700517, English

  [Refereed]

  Scientific journal


• Casein-based scaffold for artificial cellulosome design

  Geisa A.L.G. Budinova, Yutaro Mori, Tsutomu Tanaka, Noriho Kamiya

  Elsevier Ltd, Mar. 2018, Process Biochemistry, 66, 140 - 145, English

  [Refereed]

  Scientific journal


• Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode.

  Takuya Matsumoto, Yuki Isogawa, Tsutomu Tanaka, Akihiko Kondo

  A tetrameric streptavidin (SA)-appended LPETG tag was site-specifically linked to azido-containing tri-glycine via sortase A catalysis and the resulting azido-modified SA (SA-N3) was retained in the biotin-binding pocket. SA-N3 was polymerized with dibenzylcyclooctyne-modified branched poly(ethyleneglycol) (DBCO-PEG) using azido-modified branched PEG (N3-PEG) as a spacer via copper-free click chemistry. The resulting SA-based hydrogel exhibited gel-like mechanical properties and could immobilize biotin-modified molecules through biotin-SA affinity. Glucose dehydrogenase (GDH) was immobilized in the SA-based hydrogel, and the hydrogel was then coated on a glassy carbon electrode (GCE) and used for the biocatalytic oxidation of glucose. The designed GCE exhibited better performance and stability compared with GDH chemically adsorbed onto a GCE. In addition, the designed GCE anode and a Pt-carbon cathode were assembled into a glucose/O2 fuel cell that provided a maximum power density and open circuit voltage of 11.8±0.56µWcm-2 and 0.17V, respectively.

  Jan. 2018, Biosensors & bioelectronics, 99, 56 - 61, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of S. pombe via CRISPR-CAS9 genome editing for 3-hydroxypropionic acid production from glucose and cellobiose

  Tanaka, T., Kondo, A.

  2018, Basic & Clinical Pharmacology & Toxicology, 124, 17 - 17

  Scientific journal


• Metabolic design of Escherichia coli for production of shikimate pathway derivatives

  Fujiwara, R., Noda, S., Tanaka, T., Kondo, A.

  2018, Basic & Clinical Pharmacology & Toxicology, 124, 18 - 18

  Scientific journal


• Metabolic design of Escherichia coli for muconic acid production

  Fujiwara, R., Noda, S., Tanaka, T., Kondo, A.

  2018, Febs Open Bio, 8, 180 - 180

  Scientific journal


• Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux.

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Metabolic engineering has been an important approach for microbial bio-production. To produce bio-chemicals with engineered microorganisms, metabolic pathways have been edited using several common strategies, including gene disruption, gene overexpression, and gene attenuation. Here, we demonstrated metabolic channeling based on enzymatic metabolic enzyme ligation as a noteworthy approach for enhancing a desired metabolic flux. To achieve metabolic channeling , the metabolic enzymes should be in close proximity in cells. In the literature, several methodologies have been recently applied to achieve metabolic channeling . Meanwhile, we have proposed a strategy for possessing metabolic enzymes in close proximity, by utilizing sortase A as a stapler to tether such enzymes in Escherichia coli. By tethering metabolic enzymes that catalyze the reactions before and after a target metabolite, the metabolic flux may be enhanced. This chapter describes the approach for enhancing acetate-producing flux by sortase-A-assisted metabolic ligation in E. coli.

  2018, Methods in molecular biology (Clifton, N.J.), 1772, 125 - 136, English, International magazine

  [Refereed]

  Scientific journal


• Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode

  Takuya Matsumoto, Yuki Isogawa, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2018, BIOSENSORS & BIOELECTRONICS, 99, 56 - 61, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of Schizosaccharomyces pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose.

  Aiko Ozaki, Rie Konishi, Chisako Otomo, Mayumi Kishida, Seiya Takayama, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Modification of the Schizosaccharomyces pombe genome is often laborious, time consuming due to the lower efficiency of homologous recombination. Here, we constructed metabolically engineered S. pombe strains using a CRISPR-Cas9 system and also demonstrated D-lactic acid (D-LA) production from glucose and cellobiose. Genes encoding two separate pyruvate decarboxylases (PDCs), an L-lactic acid dehydrogenase (L-LDH), and a minor alcohol dehydrogenase (SPBC337.11) were disrupted, thereby attenuating ethanol production. To increase the cellular supply of acetyl-CoA, an important metabolite for growth, we introduced genes encoding bacterial acetylating acetaldehyde dehydrogenase enzymes (Escherichia coli MhpF and EutE). D-LA production by the resulting strain was achieved by expressing a Lactobacillus plantarum gene encoding D-lactate dehydrogenase. The engineered strain efficiently consumed glucose and produced D-LA at 25.2 g/L from 35.5 g/L of consumed glucose with a yield of 0.71 g D-LA / g glucose. We further modified this strain by expressing beta-glucosidase by cell surface display; the resulting strain produced D-LA at 24.4 g/L from 30 g/L of cellobiose in minimal medium, with a yield of 0.68 g D-LA / g glucose. To our knowledge, this study represents the first report of a S. pombe strain that was metabolically engineered using a CRISPR-Cas9 system, and demonstrates the possibility of engineering S. pombe for the production of value-added chemicals.

  Dec. 2017, Metabolic engineering communications, 5, 60 - 67, English, International magazine

  [Refereed]

  Scientific journal


• Engineering metabolic pathways in Escherichia coli for constructing a "microbial chassis" for biochemical production.

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  The present work reviews literature describing the re-design of the metabolic pathways of a microbial host using sophisticated genetic tools, yielding strains for producing value-added chemicals including fuels, building-block chemicals, pharmaceuticals, and derivatives. This work employed Escherichia coli, a well-studied microorganism that has been successfully engineered to produce various chemicals. E. coli has several advantages compared with other microorganisms, including robustness, and handling. To achieve efficient productivities of target compounds, an engineered E. coli should accumulate metabolic precursors of target compounds. Multiple researchers have reported the use of pathway engineering to generate strains capable of accumulating various metabolic precursors, including pyruvate, acetyl-CoA, malonyl-CoA, mevalonate and shikimate. The aim of this review provides a promising guideline for designing E. coli strains capable of producing a variety of useful chemicals. Herein, the present work reviews their common and unique strategies, treating metabolically engineered E. coli as a "microbial chassis".

  Dec. 2017, Bioresource technology, 245(Pt B) (Pt B), 1362 - 1368, English, International magazine

  [Refereed]

  Scientific journal


• Engineering metabolic pathways in Escherichia coli for constructing a "microbial chassis" for biochemical production

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(Pt B) (Pt B), 1362 - 1368, English

  [Refereed]


• 1,5-Diaminopentane production from xylooligosaccharides using metabolically engineered Corynebacterium glutamicum displaying beta-xylosidase on the cell surface

  Kenta Imao, Rie Konishi, Mayumi Kishida, Yuuki Hirata, Shota Segawa, Noriko Adachi, Rena Matsuura, Yota Tsuge, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(Pt B) (Pt B), 1684 - 1691, English

  [Refereed]

  Scientific journal


• A DNA-gold nanoparticle hybrid hydrogel network prepared by enzymatic reaction

  Yuka Eguchi, Tomoharu Kato, Tsutomu Tanaka, Tatsuo Maruyama

  May 2017, CHEMICAL COMMUNICATIONS, 53(43) (43), 5802 - 5805, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of optically pure D-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum

  Kenji Okano, Shinji Hama, Maki Kihara, Hideo Noda, Tsutomu Tanaka, Akihiko Kondo

  Mar. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(5) (5), 1869 - 1875, English

  [Refereed]

  Scientific journal


• Production of optically pure D-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum

  Kenji Okano, Shinji Hama, Maki Kihara, Hideo Noda, Tsutomu Tanaka, Akihiko Kondo

  Mar. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(5) (5), 1869 - 1875, English

  [Refereed]

  Scientific journal


• Styrene production from a biomass-derived carbon source using a coculture system of phenylalanine ammonia lyase and phenylacrylic acid decarboxylase-expressing Streptomyces lividans transformants

  Ryosuke Fujiwara, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2016, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(6) (6), 730 - 735, English

  [Refereed]

  Scientific journal


• Sortase A-Mediated Metabolic Enzyme Ligation in Escherichia coli

  Takuya Matsumoto, Kou Furuta, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2016, ACS SYNTHETIC BIOLOGY, 5(11) (11), 1284 - 1289, English

  [Refereed]

  Scientific journal


• 4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides

  Ryosuke Fujiwara, Shuhei Noda, Yoshifumi Kawai, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2016, BIOTECHNOLOGY LETTERS, 38(9) (9), 1543 - 1549, English

  [Refereed]

  Scientific journal


• 4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides

  Ryosuke Fujiwara, Shuhei Noda, Yoshifumi Kawai, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2016, BIOTECHNOLOGY LETTERS, 38(9) (9), 1543 - 1549, English

  [Refereed]

  Scientific journal


• Titanium peroxide nanoparticles enhanced cytotoxic effects of X-ray irradiation against pancreatic cancer model through reactive oxygen species generation in vitro and in vivo

  Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Tsutomu Tanaka, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Zhenquan Tan, Yuya Nishimura, Hiroaki Akasaka, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo

  Jul. 2016, RADIATION ONCOLOGY, 11(1) (1), 91, English

  [Refereed]

  Scientific journal


• Improvement of ectoine productivity by using sugar transporter-overexpressing Halomonas elongata

  Kosuke Tanimura, Takuya Matsumoto, Hideki Nakayama, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2016, ENZYME AND MICROBIAL TECHNOLOGY, 89, 63 - 68, English

  [Refereed]

  Scientific journal


• 2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis

  Kosuke Tanimura, Shingo Takashima, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(13) (13), 5781 - 5789, English

  [Refereed]

  Scientific journal


• Twigged streptavidin polymer as a scaffold for protein assembly

  Takuya Matsumoto, Yuki Isogawa, Kosuke Minamihata, Tsutomu Tanaka, Akihiko Kondo

  May 2016, JOURNAL OF BIOTECHNOLOGY, 225, 61 - 66, English

  [Refereed]

  Scientific journal


• d-lactic acid production from renewable lignocellulosic biomass via genetically modified Lactobacillus plantarum

  Yixing Zhang, Amit Kumar, Philip R. Hardwidge, Tsutomu Tanaka, Akihiko Kondo, Praveen V. Vadlani

  Mar. 2016, BIOTECHNOLOGY PROGRESS, 32(2) (2), 271 - 278, English

  [Refereed]

  Scientific journal


• One-dimensional assembly of functional proteins: toward the design of an artificial cellulosome.

  Mori Y, Nakazawa H, Gonçalves G.A.L, Tanaka T, Umetsu M, Kamiya N

  2016, Molecular Systems Design & Engineering, 1(1) (1), 66 - 73, English

  [Refereed]

  Scientific journal


• Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum

  Yixing Zhang, Praveen V. Vadlani, Amit Kumar, Philip R. Hardwidge, Revathi Govind, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(1) (1), 279 - 288, English

  [Refereed]

  Scientific journal


• Cell surface engineering of industrial microorganisms for biorefining applications

  Tsutomu Tanaka, Akihiko Kondo

  Nov. 2015, BIOTECHNOLOGY ADVANCES, 33(7) (7), 1403 - 1411, English

  [Refereed]

  Scientific journal


• C-Terminal-oriented Immobilization of Enzymes Using Sortase A-mediated Technique

  Yuto Hata, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Oct. 2015, MACROMOLECULAR BIOSCIENCE, 15(10) (10), 1375 - 1380, English

  [Refereed]

  Scientific journal


• Production of D-lactic acid from hardwood pulp by mechanical milling followed by simultaneous saccharification and fermentation using metabolically engineered Lactobacillus plantarum

  Shinji Hama, Shino Mizuno, Maki Kihara, Tsutomu Tanaka, Chiaki Ogino, Hideo Noda, Akihiko Kondo

  Jul. 2015, BIORESOURCE TECHNOLOGY, 187, 167 - 172, English

  [Refereed]

  Scientific journal


• Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse

  Lili Jia, Geisa A. L. Goncalves, Yusaku Takasugi, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Noriho Kamiya

  Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 158 - 164, English

  [Refereed]

  Scientific journal


• 新たな放射線増感剤としての過酸化チタンナノ粒子は膵癌治療における抗腫瘍効果を増強させる(Titanium Peroxide Nanoparticles, as Novel Radiosensitizers, Enhance Antitumor Efficacy in Pancreatic Cancer Therapy)

  Nakayama Masao, Sasaki Ryohei, Ogino Chiaki, Tanaka Tsutomu, Morita Kenta, Umetsu Mitsuo, Ohara Satoshi, Sato Kazuyoshi, Numako Chiya, Takami Seiichi, Kondo Akihiko

  (一社)日本放射線影響学会, May 2015, 日本放射線影響学会大会講演要旨集, 58回, 3 - 07, English


• Synergistic effect and application of xylanases as accessory enzymes to enhance the hydrolysis of pretreated bagasse

  Geisa A. L. Goncalves, Yusaku Takasugi, Lili Jia, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Noriho Kamiya

  May 2015, ENZYME AND MICROBIAL TECHNOLOGY, 72, 16 - 24, English

  [Refereed]

  Scientific journal


• Synergistic effect and application of xylanases as accessory enzymes to enhance the hydrolysis of pretreated bagasse

  Geisa A. L. Goncalves, Yusaku Takasugi, Lili Jia, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Noriho Kamiya

  May 2015, ENZYME AND MICROBIAL TECHNOLOGY, 72, 16 - 24, English

  [Refereed]

  Scientific journal


• 4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase- and tyrosine ammonia lyase-expressing Streptomyces lividans

  Shuhei Noda, Yoshifumi Kawai, Tsutomu Tanaka, Akihiko Kondo

  Mar. 2015, BIORESOURCE TECHNOLOGY, 180, 59 - 65, English

  [Refereed]

  Scientific journal


• Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology

  Tsutomu Tanaka, Akihiko Kondo

  Feb. 2015, FEMS YEAST RESEARCH, 15(1) (1), 1 - 9, English

  [Refereed]

  Scientific journal


• Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host

  Shuhei Noda, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2015, MICROBIAL CELL FACTORIES, 14(1) (1), 5, English

  [Refereed]

  Scientific journal


• Multi-functional glycoside hydrolase: Blon_0625 from Bifidobacterium longum subsp infantis ATCC 15697

  Takuya Matsumoto, Shota Shimada, Yuto Hata, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2015, ENZYME AND MICROBIAL TECHNOLOGY, 68, 10 - 14, English

  [Refereed]

  Scientific journal


• The effect of combining signal sequences with the N28 fragment on GFP production in Aspergillus oryzae

  Chiak Ogino, Tetsuya Matsuda, Fumiyoshi Okazaki, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2014, PROCESS BIOCHEMISTRY, 49(7) (7), 1078 - 1083, English

  [Refereed]

  Scientific journal


• Creation of Cellobiose and Xylooligosaccharides-Coutilizing Escherichia con Displaying both beta-Glucosidase and beta-Xylosidase on Its Cell Surface

  Tsutomu Tanaka, Yuuki Hirata, Mariko Nakano, Hitomi Kawabata, Akibiko Kondo

  Jul. 2014, ACS SYNTHETIC BIOLOGY, 3(7) (7), 446 - 453, English

  [Refereed]

  Scientific journal


• Creation of Cellobiose and Xylooligosaccharides-Coutilizing Escherichia con Displaying both beta-Glucosidase and beta-Xylosidase on Its Cell Surface

  Tsutomu Tanaka, Yuuki Hirata, Mariko Nakano, Hitomi Kawabata, Akibiko Kondo

  Jul. 2014, ACS SYNTHETIC BIOLOGY, 3(7) (7), 446 - 453, English

  [Refereed]

  Scientific journal


• Two-step production of dlactate from mixed sugars by growing and resting cells of metabolically engineered Lactobacillus plantarum

  Yota Tsuge, Hideo Kawaguchi, Kengo Sasaki, Tsutomu Tanaka, Akihiko Kondo

  Jun. 2014, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(11) (11), 4911 - 4918, English

  [Refereed]

  Scientific journal


• Lactic Acid

  Kenji Okano, Tsutomu Tanaka, Akihiko Kondo

  Wiley Blackwell, Apr. 2014, Bioprocessing of Renewable Resources to Commodity Bioproducts, 353 - 380, English

  [Refereed]

  In book


• Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions.

  Yota Tsuge, Toshihiro Tateno, Kengo Sasaki, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo

  We produced organic acids, including lactate and succinate, directly from soluble starch under anaerobic conditions using high cell-density cultures of Corynebacterium glutamicum displaying α-amylase (AmyA) from Streptococcus bovis 148 on the cell surface. Notably, reactions performed under anaerobic conditions at 35 and 40°C, which are higher than the optimal growth temperature of 30°C, showed 32% and 19%, respectively, higher productivity of the organic acids lactate, succinate, and acetate compared to that at 30°C. However, α-amylase was not stably anchored and released into the medium from the cell surface during reactions at these higher temperatures, as demonstrated by the 61% and 85% decreases in activity, respectively, from baseline, compared to the only 8% decrease at 30°C. The AmyA-displaying C. glutamicum cells retained their starch-degrading capacity during five 10 h reaction cycles at 30°C, producing 107.8 g/l of total organic acids, including 88.9 g/l lactate and 14.0 g/l succinate. The applicability of cell surface-engineering technology for the production of organic acids from biomass by high cell-density cultures of C. glutamicum under anaerobic conditions was demonstrated.

  Dec. 2013, AMB Express, 3(1) (1), 72 - 72, English, International magazine

  [Refereed]

  Scientific journal


• Two-Stage Oxidation of Glucose by an Enzymatic Bioanode

  T. Matsumoto, S. Shimada, K. Yamamoto, T. Tanaka, A. Kondo

  Dec. 2013, FUEL CELLS, 13(6) (6), 960 - 964, English

  [Refereed]

  Scientific journal


• Hyper secretion of Thermobifida fusca beta-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans

  Takaya Miyazaki, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Oct. 2013, MICROBIAL CELL FACTORIES, 12(1) (1), 88, English

  [Refereed]

  Scientific journal


• Ectoine production from lignocellulosic biomass-derived sugars by engineered Halomonas elongata

  Kosuke Tanimura, Hideki Nakayama, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2013, BIORESOURCE TECHNOLOGY, 142, 523 - 529, English

  [Refereed]

  Scientific journal


• Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface

  Noriko Adachi, Chihiro Takahashi, Naoko Ono-Murota, Rie Yamaguchi, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2013, Applied Microbiology and Biotechnology, 97(16) (16), 7165 - 7172, English

  [Refereed]

  Scientific journal


• Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source

  Shuhei Noda, Yoshifumi Kawai, Takaya Miyazaki, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(13) (13), 5711 - 5720, English

  [Refereed]

  Scientific journal


• Starchy biomass-powered enzymatic biofuel cell based on amylases and glucose oxidase multi-immobilized bioanode.

  Kazuhiro Yamamoto, Takuya Matsumoto, Shota Shimada, Tsutomu Tanaka, Akihiko Kondo

  The present study reports the design of a novel bioanode to directly utilize starch as a fuel in an enzymatic biofuel cell. The enzymatic fuel cell is based on three enzymes (alpha-amylase, glucoamylase and glucose oxidase). The carbon paste electrode containing these three enzymes and tetrathiafulvalene can both saccharize and oxidize starchy biomass. In cyclic voltammetry, catalytic currents were successfully observed with both glucose and starchy white rice used as a substrate. Finally, a membrane-less white rice/O2 biofuel cell was assembled and the electrochemical performance was evaluated. The three enzyme based electrode was used as a bioanode and an immobilized bilirubin oxidase (derived from Myrothecium verrucaria) electrode was used as a biocathode. The biofuel cell delivered an open circuit voltage of 0.522V and power density of up to 99.0 μWcm(-2). Our results show that a readily available fuel can be used for enzymatic fuel cells, and will lead to new designs.

  Jun. 2013, New biotechnology, 30(5) (5), 531 - 5, English, International magazine

  [Refereed]

  Scientific journal


• P-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans

  Yoshifumi Kawai, Shuhei Noda, Chiaki Ogino, Yasunobu Takeshima, Naoko Okai, Tsutomu Tanaka, Akihiko Kondo

  May 2013, Microbial Cell Factories, 12(1) (1), 45, English

  [Refereed]

  Scientific journal


• High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host

  Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Ogino Chiaki, Akihiko Kondo

  May 2013, Biochemical Engineering Journal, 74, 76 - 80, English

  [Refereed]

  Scientific journal


• Utilization of Lactic Acid Bacterial Genes in Synechocystis sp PCC 6803 in the Production of Lactic Acid

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Yota Tsuge, Fumio Matsuda, Tsutomu Tanaka, Akihiko Kondo

  May 2013, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(5) (5), 966 - 970, English

  [Refereed]

  Scientific journal


• Utilization of Lactic Acid Bacterial Genes in Synechocystis sp PCC 6803 in the Production of Lactic Acid

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Yota Tsuge, Fumio Matsuda, Tsutomu Tanaka, Akihiko Kondo

  May 2013, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(5) (5), 966 - 970, English

  [Refereed]

  Scientific journal


• Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins

  Tsutomu Tanaka, Sayoko Matsumoto, Mari Yamada, Ryosuke Yamada, Fumio Matsuda, Akihiko Kondo

  May 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(10) (10), 4343 - 4352, English

  [Refereed]

  Scientific journal


• Preparation of affinity membranes using thermally induced phase separation for one-step purification of recombinant proteins

  Takafumi Honjo, Kazuki Hoe, Shunsuke Tabayashi, Tsutomu Tanaka, Josui Shimada, Masahiro Goto, Hideto Matsuyama, Tatsuo Maruyama

  Mar. 2013, ANALYTICAL BIOCHEMISTRY, 434(2) (2), 269 - 274, English

  [Refereed]

  Scientific journal


• Preparation of hemispherical polymer particles via phase separation induced by microsuspension polymerization

  Yamagami, Tomoe, Tanaka, Takuya, Suzuki, Toyoko, Okubo, Masayoshi

  2013, Colloid and Polymer Science, 291(1) (1)

  Scientific journal


• Particle size for photocatalytic activity of anatase TiO2 nanosheets with highly exposed {001} facets

  Zhenquan Tan, Kazuyoshi Sato, Seiichi Takami, Chiya Numako, Mitsuo Umetsu, Kohei Soga, Masao Nakayama, Ryohei Sasaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Kazuhiro Yamamoto, Takeshi Hashishin, Satoshi Ohara

  2013, RSC ADVANCES, 3(42) (42), 19268 - 19271, English

  [Refereed]

  Scientific journal


• Single-step production of polyhydroxybutyrate from starch by using alpha-amylase cell-surface displaying system of Corynebacterium glutamicum

  Yuyang Song, Ken'ichiro Matsumoto, Tsutomu Tanaka, Akihiko Kondo, Seiichi Taguchi

  Jan. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115(1) (1), 12 - 14, English

  [Refereed]

  Scientific journal


• Aligning an endoglucanase Cel5A from Thermobifida fusca on a DNA scaffold: potent design of an artificial cellulosome

  Yutaro Mori, Shiori Ozasa, Momoko Kitaoka, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Noriho Kamiya

  2013, CHEMICAL COMMUNICATIONS, 49(62) (62), 6971 - 6973, English

  [Refereed]

  Scientific journal


• Direct cadaverine production from cellobiose using beta-glucosidase displaying Escherichia coli

  Naoki Ikeda, Mari Miyamoto, Noriko Adachi, Mariko Nakano, Tsutomu Tanaka, Akihiko Kondo

  2013, AMB EXPRESS, 3(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal


• Efficient heterologous expression and secretion in Aspergillus oryzae of a llama variable heavy-chain antibody fragment V-HH against EGFR

  Fumiyoshi Okazaki, Jun-ichi Aoki, Soichiro Tabuchi, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 96(1) (1), 81 - 88, English

  [Refereed]

  Scientific journal


• Enzyme-mediated methodologies for protein modification and bioconjugate synthesis

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2012, BIOTECHNOLOGY JOURNAL, 7(9) (9), 1137 - 1146, English

  [Refereed]

  Scientific journal


• Recent developments in yeast cell surface display toward extended applications in biotechnology

  Tsutomu Tanaka, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

  Aug. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 95(3) (3), 577 - 591, English

  [Refereed]

  Scientific journal


• Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  Yuki Soma, Kentaro Inokuma, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Masahiro Okamoto, Taizo Hanai

  Jul. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 80 - 85, English

  [Refereed]

  Scientific journal


• Site-specific protein labeling with amine-containing molecules using Lactobacillus plantarum sortase

  Takuya Matsumoto, Ryosuke Takase, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  May 2012, BIOTECHNOLOGY JOURNAL, 7(5) (5), 642 - 648, English

  [Refereed]

  Scientific journal


• Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and alpha-amylase

  Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2012, ENZYME AND MICROBIAL TECHNOLOGY, 50(6-7) (6-7), 343 - 347, English

  [Refereed]

  Scientific journal


• Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and alpha-amylase

  Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2012, ENZYME AND MICROBIAL TECHNOLOGY, 50(6-7) (6-7), 343 - 347, English

  [Refereed]

  Scientific journal


• Benzoic acid fermentation from starch and cellulose via a plant-like beta-oxidation pathway in Streptomyces maritimus

  Shuhei Noda, Eiichi Kitazono, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Apr. 2012, MICROBIAL CELL FACTORIES, 11, 49, English

  [Refereed]

  Scientific journal


• Sortase A-Catalyzed Site-Specific Coimmobilization on Microparticles via Streptavidin

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Feb. 2012, LANGMUIR, 28(7) (7), 3553 - 3557, English

  [Refereed]

  Scientific journal


• Protein-encapsulated bio-nanocapsules production with ER membrane localization sequences

  Yuya Nishimura, Takuya Shishido, Jun Ishii, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jan. 2012, JOURNAL OF BIOTECHNOLOGY, 157(1) (1), 124 - 129, English

  [Refereed]

  Scientific journal


• Production of Streptoverticillium cinnamoneum transglutaminase and cinnamic acid by recombinant Streptomyces lividans cultured on biomass-derived carbon sources

  Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jan. 2012, BIORESOURCE TECHNOLOGY, 104, 648 - 651, English

  [Refereed]

  Scientific journal


• Task-specific membranes for the isolation of recombinant proteins with peptide tags

  Tatsuo Maruyama, Shunsuke Tabayashi, Takafumi Honjo, Kazuki Hoe, Tsutomu Tanaka, Josui Shimada, Masahiro Goto, Hideto Matsuyama

  2012, RSC ADVANCES, 2(1) (1), 125 - 127, English

  [Refereed]

  Scientific journal


• Homo-D-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum

  Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92(1) (1), 67 - 76, English

  [Refereed]

  Scientific journal


• Improved homo l-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating Lactococcus lactis

  Satoru Shinkawa, Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(6) (6), 1537 - 1544, English

  [Refereed]

  Scientific journal


• Creation of a Cellooligosaccharide-Assimilating Escherichia coli Strain by Displaying Active Beta-Glucosidase on the Cell Surface via a Novel Anchor Protein

  Tsutomu Tanaka, Hitomi Kawabata, Chiaki Ogino, Akihiko Kondo

  Sep. 2011, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(17) (17), 6265 - 6270, English

  [Refereed]

  Scientific journal


• Co-fermentation of cellulose/xylan using engineered industrial yeast strain OC-2 displaying both beta-glucosidase and beta-xylosidase

  Satoshi Saitoh, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(6) (6), 1553 - 1559, English

  [Refereed]

  Scientific journal


• Glutamate production from beta-glucan using endoglucanase-secreting Corynebacterium glutamicum

  Takeyuki Tsuchidate, Toshihiro Tateno, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 895 - 901, English

  [Refereed]

  Scientific journal


• Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase

  Shuhei Noda, Takaya Miyazaki, Takanori Miyoshi, Michiru Miyake, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2011, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 38(5) (5), 643 - 648, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Apr. 2011, BIOTECHNOLOGY FOR BIOFUELS, 4, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Apr. 2011, BIOTECHNOLOGY FOR BIOFUELS, 4, 8, English

  [Refereed]

  Scientific journal


• Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases

  Ryosuke Yamada, Syun-ichi Yamakawa, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Apr. 2011, ENZYME AND MICROBIAL TECHNOLOGY, 48(4-5) (4-5), 393 - 396, English

  [Refereed]

  Scientific journal


• Site-specific tetrameric streptavidin-protein conjugation using sortase A

  Takuya Matsumoto, Shiori Sawamoto, Takayuki Sakamoto, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Mar. 2011, JOURNAL OF BIOTECHNOLOGY, 152(1-2) (1-2), 37 - 42, English

  [Refereed]

  Scientific journal


• Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans

  Chiaki Ogino, Shuhei Noda, Akihiko Kondo, Naoko Okai, Tsutomu Tanaka

  2011, 2011 Defense Science Research Conference and Expo, DSR 2011, English

  [Refereed]

  International conference proceedings


• Homo D-lactic acid production from xylose/glucose mixture using xylose-assimilating operon integrated Lactobacillus plantrum

  YOSHIDA Shogo, OKANO Kenji, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  2011, Applied Microbiology and Biotechnology, 92(1), 67-76, English

  [Refereed]

  Scientific journal


• Enzyme-Mediated Site-Specific Antibody-Protein Modification Using a ZZ Domain as a Linker

  Takayuki Sakamoto, Shiori Sawamoto, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Dec. 2010, BIOCONJUGATE CHEMISTRY, 21(12) (12), 2227 - 2233, English

  [Refereed]

  Scientific journal


• Preparation of monodispersed polyelectrolyte microcapsules with high encapsulation efficiency by an electrospray technique

  Yu Fukui, Tatsuo Maruyama, Yuko Iwamatsu, Akihiro Fujii, Tsutomu Tanaka, Yoshikage Ohmukai, Hideto Matsuyama

  Nov. 2010, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 370(1-3) (1-3), 28 - 34, English

  [Refereed]

  Scientific journal


• Over-production of various secretory-form proteins in Streptomyces lividans

  Shuhei Noda, Yuko Ito, Nobuaki Shimizu, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2010, PROTEIN EXPRESSION AND PURIFICATION, 73(2) (2), 198 - 202, English

  [Refereed]

  Scientific journal


• Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells

  Takuya Shishido, Hiroaki Mieda, Sang Youn Hwang, Yuya Nishimura, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Oct. 2010, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20(19) (19), 5726 - 5731, English

  [Refereed]

  Scientific journal


• Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts

  Risa Koda, Takao Numata, Shinji Hama, Sriappareddy Tamalampudi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 66(1-2) (1-2), 101 - 104, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes

  Shuhei Yanase, Tomohisa Hasunuma, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(1) (1), 381 - 388, English

  [Refereed]

  Scientific journal


• Biofunctional TiO2 nanoparticle-mediated photokilling of cancer cells using UV irradiation

  Kazusa Matsui, Miki Karasaki, Maiko Segawa, Sang Youn Hwang, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Sep. 2010, MEDCHEMCOMM, 1(3) (3), 209 - 211, English

  [Refereed]

  Scientific journal


• Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module

  Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Fumiyoshi Okazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1783 - 1789, English

  [Refereed]

  Scientific journal


• Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2

  Satoshi Saitoh, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1975 - 1982, English

  [Refereed]

  Scientific journal


• Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain

  Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(1) (1), 109 - 115, English

  [Refereed]

  Scientific journal


• Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling

  Shota Togawa, Jun Ishii, Atsushi Ishikura, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 867 - 873, English

  [Refereed]

  Scientific journal


• Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast

  Yusuke Iguchi, Jun Ishii, Hideki Nakayama, Atsushi Ishikura, Keiko Izawa, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 875 - 884, English

  [Refereed]

  Scientific journal


• Ethanol production from cellulosic materials using cellulase-expressing yeast

  Shuhei Yanase, Ryosuke Yamada, Shohei Kaneko, Hideo Noda, Tomohisa Hasunuma, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, BIOTECHNOLOGY JOURNAL, 5(5) (5), 449 - 455, English

  [Refereed]

  Scientific journal


• Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, MICROBIAL CELL FACTORIES, 9, 32, English

  [Refereed]

  Scientific journal


• The competitor-introduced G gamma recruitment system, a new approach for screening affinity-enhanced proteins

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Akihiko Kondo

  Apr. 2010, FEBS JOURNAL, 277(7) (7), 1704 - 1712, English

  [Refereed]

  Scientific journal


• Fatty acid production from butter using novel cutinase-displaying yeast

  Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Atsushi Kotaka, Hiroshi Sahara, Tsuneharu Hashimoto, Nobuyuki Kuratani, Seiji Shibasaki, Chiaki Ogino, Hideo Noda, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Mar. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(3-4) (3-4), 194 - 199, English

  [Refereed]

  Scientific journal


• Novel strategy for yeast construction using delta-integration and cell fusion to efficiently produce ethanol from raw starch

  Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(5) (5), 1491 - 1498, English

  [Refereed]

  Scientific journal


• D-lactic acid production from cellooligosaccharides and beta-glucan using L-LDH gene-deficient and endoglucanase-secreting Lactobacillus plantarum

  Kenji Okano, Qiao Zhang, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 643 - 650, English

  [Refereed]

  Scientific journal


• Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction

  Tsutomu Tanaka, Shinsuke Masunari, Jun Ishii, Kanako Wakamura, Maiko Segawa, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, JOURNAL OF BIOTECHNOLOGY, 145(1) (1), 79 - 83, English

  [Refereed]

  Scientific journal


• Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits

  Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 413 - 423, English

  [Refereed]

  Scientific journal


• Improved Production of Homo-D-Lactic Acid via Xylose Fermentation by Introduction of Xylose Assimilation Genes and Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

  Kenji Okano, Shogo Yoshida, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24) (24), 7858 - 7861, English

  [Refereed]

  Scientific journal


• Biotinylated Bionanocapsules for Displaying Diverse Ligands Toward Cell-specific Delivery

  Takuya Shishido, Yuki Azumi, Takeshi Nakanishi, Mitsuo Umetsu, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2009, JOURNAL OF BIOCHEMISTRY, 146(6) (6), 867 - 874, English

  [Refereed]

  Scientific journal


• Development of novel cell surface display in Corynebacterium glutamicum using porin

  Toshihiro Tateno, Kazuki Hatada, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Sep. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(4) (4), 733 - 739, English

  [Refereed]

  Scientific journal


• Improvement of isoflavone aglycones production using beta-glucosidase secretory produced in recombinant Aspergillus oryzae

  Ken Horii, Takashi Adachi, Tetsuya Matsuda, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Aug. 2009, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 59(4) (4), 297 - 301, English

  [Refereed]

  Scientific journal


• Homo-D-Lactic Acid Fermentation from Arabinose by Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

  Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Aug. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(15) (15), 5175 - 5178, English

  [Refereed]

  Scientific journal


• Evaluation of cell surface-displayed protein stability against simulated gastric fluid

  Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Aug. 2009, BIOTECHNOLOGY LETTERS, 31(8) (8), 1259 - 1264, English

  [Refereed]

  Scientific journal


• Development of an enzyme activity screening system for beta-glucosidase-displaying yeasts using calcium alginate micro-beads and flow sorting

  Yan Zhou, Shin'ichiro Kajiyama, Kouichi Itoh, Takanori Tanino, Nobuo Fukuda, Tsutomu Tanaka, Akihiko Kondo, Kiichi Fukui

  Aug. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(2) (2), 375 - 382, English

  [Refereed]

  Scientific journal


• Regulation of the Display Ratio of Enzymes on the Saccharomyces cerevisiae Cell Surface by the Immunoglobulin G and Cellulosomal Enzyme Binding Domains

  Junji Ito, Akihiko Kosugi, Tsutomu Tanaka, Kouichi Kuroda, Seiji Shibasaki, Chiaki Ogino, Mitsuyoshi Ueda, Hideki Fukuda, Roy H. Doi, Akihiko Kondo

  Jun. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(12) (12), 4149 - 4154, English

  [Refereed]

  Scientific journal


• Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae

  Shohei Kaneko, Tsutomu Tanaka, Hideo Noda, Hideki Fukuda, Rinji Akada, Akihiko Kondo

  Jun. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 83(4) (4), 783 - 789, English

  [Refereed]

  Scientific journal


• A Simple and Immediate Method for Simultaneously Evaluating Expression Level and Plasmid Maintenance in Yeast

  Jun Ishii, Keiko Izawa, Shizuka Matsumura, Kanako Wakamura, Takanori Tanino, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jun. 2009, JOURNAL OF BIOCHEMISTRY, 145(6) (6), 701 - 708, English

  [Refereed]

  Scientific journal


• Efficient production of ethanol from raw starch by a mated diploid Saccharomyces cerevisiae with integrated alpha-amylase and glucoamylase genes

  Ryosuke Yamada, Yohei Bito, Takashi Adachi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2009, ENZYME AND MICROBIAL TECHNOLOGY, 44(5) (5), 344 - 349, English

  [Refereed]

  Scientific journal


• Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  May 2009, FEBS JOURNAL, 276(9) (9), 2636 - 2644, English

  [Refereed]

  Scientific journal


• Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  May 2009, FEBS JOURNAL, 276(9) (9), 2636 - 2644, English

  [Refereed]

  Scientific journal


• A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

  Takuya Shishido, Naoya Kurata, Myung Eui Yoon, Tsutomu Tanaka, Hideki Yamaji, Hideki Fukuda, Akihiko Kondo

  May 2009, BIOTECHNOLOGY LETTERS, 31(5) (5), 623 - 627, English

  [Refereed]

  Scientific journal


• Construction of arginine-rich peptide displaying bionanocapsules

  Takuya Shishido, Daisaku Yonezawa, Kiyokazu Iwata, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Mar. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(5) (5), 1473 - 1476, English

  [Refereed]

  Scientific journal


• Direct production of cadaverine from soluble starch using Corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase

  Toshihiro Tateno, Yusuke Okada, Takeyuki Tsuchidate, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Feb. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(1) (1), 115 - 121, English

  [Refereed]

  Scientific journal


• CONSTRUCTION OF A NOVEL DETECTION SYSTEM FOR PROTEIN-PROTEIN INTERACTIONS USING YEAST G-PROTEIN SIGNALING

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  2009, IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS, 238 - 238, English

  [Refereed]

  International conference proceedings


• Efficient Production of Optically Pure D-Lactic Acid from Raw Corn Starch by Using a Genetically Modified L-Lactate Dehydrogenase Gene-Deficient and alpha-Amylase-Secreting Lactobacillus plantarum Strain

  Kenji Okano, Qiao Zhang, Satoru Shinkawa, Shogo Yoshida, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Jan. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(2) (2), 462 - 467, English

  [Refereed]

  Scientific journal


• Specific Protein Delivery to Target Cells by Antibody-displaying Bionanocapsules

  Naoya Kurata, Takuya Shishido, Masaru Muraoka, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2008, JOURNAL OF BIOCHEMISTRY, 144(6) (6), 701 - 707, English

  [Refereed]

  Scientific journal


• Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple beta-Glucosidase Genes

  Satoshi Saitoh, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(6) (6), 594 - 597, English

  [Refereed]

  Scientific journal


• Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating S-cerevisiae via expression of beta-glucosidase on its cell surface

  Nobuhiro Nakamura, Ryosuke Yamada, Satoshi Katahira, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Sep. 2008, ENZYME AND MICROBIAL TECHNOLOGY, 43(3) (3), 233 - 236, English

  [Refereed]

  Scientific journal


• Improvement of ethanol productivity during xylose and glucose co-fermentation by xylose-assimilating S. cerevisiae via expression of glucose transporter Sut1

  Satoshi Katahira, Meguru Ito, Hisae Takema, Yasuya Fujita, Takanori Tanino, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Aug. 2008, ENZYME AND MICROBIAL TECHNOLOGY, 43(2) (2), 115 - 119, English

  [Refereed]

  Scientific journal


• Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast

  Jun Ishii, Tsutomu Tanaka, Shizuka Matsumura, Kenji Tatematsu, Shun'ichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2008, JOURNAL OF BIOCHEMISTRY, 143(5) (5), 667 - 674, English

  [Refereed]

  Scientific journal


• Analysis of human oral mucosa ex vivo for fatty acid compositions using fourier-transform infrared spectroscopy

  Satoshi Yoshida, Yuhki Okazaki, Takumi Yamashita, Hiroshi Ueda, Reza Ghadimi, Akihiro Hosono, Tsutomu Tanaka, Kiyonori Kuriki, Sadao Suzuki, Shinkan Tokudome

  Apr. 2008, LIPIDS, 43(4) (4), 361 - 372, English

  [Refereed]

  Scientific journal


• Analysis of human oral mucosa ex vivo for fatty acid compositions using fourier-transform infrared spectroscopy

  Satoshi Yoshida, Yuhki Okazaki, Takumi Yamashita, Hiroshi Ueda, Reza Ghadimi, Akihiro Hosono, Tsutomu Tanaka, Kiyonori Kuriki, Sadao Suzuki, Shinkan Tokudome

  Apr. 2008, LIPIDS, 43(4) (4), 361 - 372, English

  [Refereed]

  Scientific journal


• Site-specific protein modification on living cells catalyzed by Sortase

  Tsutomu Tanaka, Teruyasu Yamamoto, Shinya Tsukiji, Teruyuki Nagamune

  Mar. 2008, CHEMBIOCHEM, 9(5) (5), 802 - 807, English

  [Refereed]

  Scientific journal


• Site-specific protein modification on living cells catalyzed by Sortase

  Tsutomu Tanaka, Teruyasu Yamamoto, Shinya Tsukiji, Teruyuki Nagamune

  Mar. 2008, CHEMBIOCHEM, 9(5) (5), 802 - 807, English

  [Refereed]

  Scientific journal


• Rapid and efficient selection of yeast displaying a target protein using thermo-responsive magnetic nanoparticles

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Hideki Fukuda, Noriyuki Ohnishi, Akihiko Kondo

  Mar. 2008, BIOTECHNOLOGY PROGRESS, 24(2) (2), 352 - 357, English

  [Refereed]

  Scientific journal


• System using tandem repeats of the cA peptidoglycan-binding domain from Lactococcus lactis for display of both N- and C-terminal fusions on cell surfaces of lactic acid bacteria

  Kenji Okano, Qiao Zhang, Sakurako Kimura, Junya Narita, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Feb. 2008, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(4) (4), 1117 - 1123, English

  [Refereed]

  Scientific journal


• Novel cell surface display on lactic acid bacteria and its application to lactic acid production from starchy materials

  Kenji Okano, Qiao Zhang, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  2008, AIChE Annual Meeting, Conference Proceedings, English

  International conference proceedings


• 抗体提示バイオナノカプセルを用いたピンポイントプロテインデリバリーシステムの開発

  安住 友希, 倉田 直弥, 宍戸 卓矢, 上田 政和, 妹尾 昌治, 多田 宏子, 谷澤 克行, 黒田 俊一, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 320 - 320, Japanese


• ペプチド転移酵素を用いた細胞内タンパク質連結技術の開発

  若村 香菜子, 田中 勉, 石井 純, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 944 - 944, Japanese


• 酵母蛍光レポーターアッセイによるヒトソマトスタチンレセプター変異体の機能解析

  戸川 翔太, 石井 純, 田中 勉, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 971 - 971, Japanese


• 酵母でのヒトG蛋白質共役型受容体発現に関する分泌シグナル配列の影響

  井口 裕介, 石井 純, 田中 勉, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 970 - 970, Japanese


• セルラーゼ発現酵母によるセルロースからのバイオエタノール生産

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 990 - 990, Japanese


• 生デンプンからの高効率D-乳酸生産技術の開発

  新川 智, 岡野 憲司, 張 イ肖, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 988 - 988, Japanese


• Whole-cell biocatalystを使用した菜種油からのバイオディーゼル生産

  甲田 梨沙, 田中 勉, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 997 - 997, Japanese


• 部分グリセリドリパーゼを用いたバイオディーゼル燃料生産系の開発

  甲田 梨沙, 沼田 崇男, 田中 勉, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 399 - 399, Japanese


• C5・C6糖からの効率的なエタノール生産技術の開発

  阿部 祐一郎, 山田 亮介, 中村 延博, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 394 - 394, Japanese


• Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure

  Hidehiko Hirakawa, Noriho Kamiya, Tsutomu Tanaka, Teruyuki Nagamune

  Sep. 2007, PROTEIN ENGINEERING DESIGN & SELECTION, 20(9) (9), 453 - 459, English

  [Refereed]

  Scientific journal


• Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure

  Hidehiko Hirakawa, Noriho Kamiya, Tsutomu Tanaka, Teruyuki Nagamune

  Sep. 2007, Protein Engineering, Design and Selection, 20(9) (9), 453 - 459, English

  [Refereed]

  Scientific journal


• 酵素を用いた細胞表層膜蛋白質の部位特異的ラベリング技術の開発

  山本 晃康, 田中 勉, 築地 真也, 長棟 輝行

  (公社)日本生物工学会, Aug. 2007, 日本生物工学会大会講演要旨集, 平成19年度, 64 - 64, Japanese

  [Refereed]


• 酵素を用いた蛋白質環状化技術の開発

  田中 勉, 築地 真也, 長棟 輝行

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 410 - 410, Japanese


• 蛋白質改変技術を指向した新規分割型インテインの合理的創製

  安東 友美, 田中 勉, 築地 真也, 長棟 輝行

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 412 - 412, Japanese


• 酵素を用いた細胞表層膜蛋白質のC末端特異的ラベリング技術の開発

  山本 晃康, 田中 勉, 築地 真也, 長棟 輝行

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 411 - 411, Japanese


• Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation

  Tomomi Ando, Shinya Tsukiji, Tsutomu Tanaka, Teruyuki Nagamune

  2007, CHEMICAL COMMUNICATIONS, 47(47) (47), 4995 - 4997, English

  [Refereed]

  Scientific journal


• Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation

  Tomomi Ando, Shinya Tsukiji, Tsutomu Tanaka, Teruyuki Nagamune

  2007, CHEMICAL COMMUNICATIONS, (47) (47), 4995 - 4997, English

  [Refereed]

  Scientific journal


• N-terminal glycine-specific protein conjugation catalyzed by microbial transglutaminase

  T Tanaka, N Kamiya, T Nagamune

  Apr. 2005, FEBS LETTERS, 579(10) (10), 2092 - 2096, English

  [Refereed]

  Scientific journal


• Peptidyl linkers for protein heterodimerization catalyzed by microbial transglutaminase

  Tsutomu Tanaka, Noriho Kamiya, Teruyuki Nagamune

  May 2004, Bioconjugate Chemistry, 15(3) (3), 491 - 497, English

  [Refereed]

  Scientific journal


• A Peptidyl Linker for Protein Cross-Linking Catalyzed by Microbial Transglutaminase

  Tanaka Tsutomu, Kamiya Noriho, Nagamune Teruyuki

  We found that some kind of peptidyl linkers can work as peptidyl tags for microbial transglutaminase (MTG)-mediated protein heterodimerization. The Myc epitope (the amino acid sequence: EQKLISEEL) and the T7 epitope (MASMTGGQQMG) that were attached to the N-terminus of enhanced green fluorescent protein (EGFP) were found to serve Gln-tags (i.e. peptidyl tags that provide reactive Gln residues for MTG-mediated protein cross-linking). A calmodulin binding peptide (KRRWKKNFIAVSAANRFKKISSSGAL), the V5 epitope (GKPIPNPLLGLD-ST) and the Strep-tag II (WSHPQFEK) that were attached to the N-terminus of EGFP were found to be Lys-tags that provide reactive Lys residues for the enzymatic cross-linking. The specific Gln-Lys cross-linkage was formed through Gln- and Lys-tags by MTG and the major products were EGFP heterodimers. The reactivity of peptidyl linkers containing reactive Lys residue decreased when they were tethered to C-terminus of EGFP, indicating that the microenvironment or steric hindrance affects more the substrate recognition of MTG than their amino acid sequences. It was found that the insertion of a specific peptide sequence (FERQHMDS, a part of ribonuclease S-peptide) into a loop of EGFP could facilitate the protein cross-linking reaction. These results suggest that commercial peptidyl tags can be employed as specific cross-linkers for MTG-mediated site-specific protein ligation.

  The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 377 - 377, English


• Site-specific cross-linking of functional proteins by transglutamination

  Kamiya, N., Takazawa, T., Tanaka, T., Ueda, H., Nagamune, T.

  Sep. 2003, Enzyme and Microbial Technology, 33(4) (4), 492 - 496, English

  [Refereed]

  Scientific journal


• S-peptide as a potent peptidyl linker for protein cross-linking by microbial transglutaminase from Streptomyces mobaraensis

  N Kamiya, T Tanaka, T Suzuki, T Takazawa, S Takeda, K Watanabe, T Nagamune

  Mar. 2003, BIOCONJUGATE CHEMISTRY, 14(2) (2), 351 - 357, English

  [Refereed]

  Scientific journal


• 酵素法によるタンパク質へテロ二量化のための特異的ペプチドリンカーの創製

  田中 勉, 神谷 典穂, 長棟 輝行

  公益社団法人 化学工学会, 2003, 化学工学会 研究発表講演要旨集, 2003, 674 - 674, Japanese

• Cost-effective production of D-lactic acid from corncobs by simultaneous saccharification and fermentation using genetically engineered lactic acid bacteria

  久保早友理, 熊田陽一, 堀内淳一, 岡野憲司, 近藤昭彦, 田中勉

  2022, 日本生物工学会大会講演要旨集, 74th


• 未利用竹資源を活用したを用いたアスタキサンチン及びD-乳酸の微生物生産

  津田哲昌, 熊田陽一, 堀内淳一, 岡野憲司, 近藤昭彦, 田中勉

  2020, 化学工学会秋季大会研究発表講演要旨集(CD-ROM), 51st


• 未利用バイオマスからのD-乳酸生産に向けた乳酸菌の代謝工学研究

  岡野憲司, 濵真司, 田中勉, 野田秀夫, KONDO Akihiko

  農林水産・食品産業技術振興協会, 2017, JATAFFジャーナル, 5(3) (3), 33 - 37, Japanese

  [Refereed]

  Introduction scientific journal


• Synergistic degradation of arabinoxylan by free and immobilized xylanases and arabinofuranosidase

  Lili Jia, Geisa A. L. G. Budinova, Yusaku Takasugi, Shuhei Noda, Tsutomu Tanaka, Hirofumi Ichinose, Masahiro Goto, Noriho Kamiya

  Oct. 2016, BIOCHEMICAL ENGINEERING JOURNAL, 114, 271 - 278, English


• Putrescine production from cellobiose by cell surface- and metabolically-engineered E. coli

  Tsutomu Tanaka, Hiroki Nishikawa, Naoki Ikeda, Akihiko Kondo

  Jul. 2016, NEW BIOTECHNOLOGY, 33, S191 - S191, English

  Summary international conference


• Metabolic enzyme ligation by sortagging

  Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  Jul. 2016, NEW BIOTECHNOLOGY, 33, S194 - S194, English

  Summary international conference


• 2P-195 Development of metabolic channeling for efficient production of butanol using Escherichia coli.

  Furuta Kou, Matsumoto takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 223 - 223, Japanese


• 2P-196 C-Terminal-oriented Immobilization of Enzymes Using Sortase A-mediated Technique

  Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 223 - 223, Japanese


• 2P-183 An efficient biomass degradation system by using synergistic action of accessory enzymes

  Lili Jia, Geisa A.L.G. Budinova, Yusaku Takasugi, Tsutomu Tanaka, Hirofumi Ichinose, Noriho Kamiya

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 220 - 220, English


• 2P-014 Cadaverine production from biomss using Corynebacterium glutamicum

  Imao Kenta, Segawa Shota, Tanaka Tsutomu, Kondou Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 178 - 178, Japanese


• Co-assimilation of cellobiose and xylooligosaccharides using E. coli displaying both beta-glucosidase and beta-xylosidase on its cell surface

  Tsutomu Tanaka, Mariko Nakano, Yuuki Hirata, Akihiko Kondo

  Sep. 2014, JOURNAL OF BIOTECHNOLOGY, 185, S52 - S52, English

  Summary international conference


• 過酸化チタンナノ粒子による放射線増感の検討

  中山 雅央, 佐々木 良平, 西村 英輝, 吉田 賢史, 宮脇 大輔, 赤坂 浩亮, 椋本 成俊, 村岡 修, 原田 文, ノル・シャズリナ, 上原 和之, 田中 勉, 荻野 千秋, 近藤 昭彦

  (公社)日本医学放射線学会, Feb. 2014, Japanese Journal of Radiology, 32(Suppl.) (Suppl.), 37 - 37, Japanese


• 3P-227 Enzymatic degradation of cellulose/hemicellulose with multi-functional beta-glucosidase

  Matsumoto Takuya, Hata Yuto, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 251 - 251, Japanese


• 3P-224 Study of synergistic effect between xylanases for high efficient degradation of cellulosic biomass

  Takasugi Yusaku, Mori Yutaro, Jia Lili, L. Goncalves Geisa A., Hikino Yukie, Tanaka Tsutomu, Ichinose Hirofumi, Kamiya Noriho

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 250 - 250, Japanese


• 3P-225 Highly-oriented immobilized enzymes using sortase A-mediated transpeptidation

  Hata Yuto, Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 251 - 251, Japanese


• 2P-110 Direct cadaverine production from oligosaccharide using Escherichia coli coexpressing beta-glucosidase and beta-xylosidase

  Ikeda Naoki, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 134 - 134, Japanese


• 2P-094 Analysis of high-yield, high-efficient homo-butanol-fermentative Clostridia

  Mukoyama Masaharu, Nananosho Masahiro, Ichige Eita, Dobashi Yukio, Nakayama Shunichi, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 130 - 130, Japanese


• 2P-112 Lactic acid production from cellobiose using Schizosaccharomyces pombe displaying beta-glucosidase on the surface

  Ogawa Kousuke, Matsumoto Sayoko, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 134 - 134, Japanese


• Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 岡本 正宏, 花井 泰三

  日本生物工学会, 2014, 生物工学会誌 : seibutsu-kogaku kaishi, 92(2) (2), 71 - 71, Japanese


• コリネ菌の細胞表層提示技術を用いたデンプンからの乳酸様ポリマーの生産

  KADOYA KYOSUKE, SONG YUYANG, TOBITANI KOTA, MATSUMOTO KEN'ICHIRO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI

  27 Nov. 2013, 日本農芸化学会北海道支部講演会講演要旨, 2013, 38, Japanese


• デンプンを炭素源に用いたポリ乳酸様ポリマーの合成

  KADOYA RYOSUKE, SONG YUYANG, TOBITANI KOTA, MATSUMOTO KEN'ICHIRO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI

  日本生物工学会, 25 Aug. 2013, 日本生物工学会大会講演要旨集, 65th, 238 - 238, Japanese


• 2P-089 Highly-oriented immobilized cellulase using sortase A-mediated transpeptidation

  Hata Yuto, Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 126 - 126, Japanese


• 3P-182 Glucose dehydrogenase and gluconate dehydrogenase co-immobilized bioanode for biofuel cell

  Shimada Shota, Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 233 - 233, Japanese


• 3P-161 Direct putrescine production from cellobiose using Escherichia coli displaying beta-glucosidase

  Ikeda Naoki, Miyamoto Mari, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 228 - 228, Japanese


• 3P-183 Oriented co-immobilization of enzymes through sortase A mediated-methodology

  Matsumoto Takuya, Hata Yuto, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 234 - 234, Japanese


• Novel Radiosensitization Through Reactive Oxygen Species Generation Using Titanium Peroxide Nanoparticle Compounds Against Pancreas Cancer

  M. Nakayama, R. Sasaki, C. Ogino, T. Tanaka, M. Umetsu, S. Ohara, Z. Tan, K. Sato, C. Numako, A. Kondo

  Nov. 2012, INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 84(3) (3), S700 - S700, English

  Summary international conference


• 糖質バイオマスから多様なポリエステルを生産するコリネ菌微生物工場の開発

  SONG YUYANG, MATSUMOTO KEN'ICHIRO, OI TOSHIHIKO, TANAKA TSUTOMU, KONDO AKIHIKO, TAGUCHI SEIICHI

  日本生物工学会, 25 Sep. 2012, 日本生物工学会大会講演要旨集, 64th, 27 - 27, Japanese


• Benzoic acid fermentation from starch and cellulose via a plant-like beta-oxidation pathway in Streptomyces maritimus

  Shuhei Noda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Sep. 2012, NEW BIOTECHNOLOGY, 29, S50 - S50, English

  Summary international conference


• Sortase-mediated protein modification and utilization for biotechnological application

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2012, NEW BIOTECHNOLOGY, 29, S97 - S98, English

  Summary international conference


• Cellooligosaccharide assimilating E. coli by displaying active BGL on the cell surface using novel anchor protein

  Tsutomu Tanaka, Yuuki Hirata, Hitomi Kawabata, Akihiko Kondo

  Sep. 2012, NEW BIOTECHNOLOGY, 29, S51 - S51, English

  Summary international conference


• 4Hp09 Cellulase and glucose dehydrogenase co-immobilized bioanode for biofuel cell

  Shimada Shota, Yamamoto Kazuhiro, Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 234 - 234, Japanese


• 4Ha05 Construction of biomass-degradation enzymes library from Streptomyces and exploring novel high-level secretion signal

  Miyazaki Takaya, Noda Shuhei, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 230 - 230, Japanese


• 3Hp19 Production of ectoine from biomass using Halomonas elongata

  Tanimura Kosuke, Nakayama Hideki, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 156 - 156, Japanese


• 3Hp07 Production of L-Lysine from cellobiose using Corynebacterium glutamicum expressing beta-glucosidase

  ADACHI Noriko, ONO Naoko, HIRATA Yuuki, TAKAHASHI Chihiro, OKAI Naoko, TANAKA Tsutomu, KONDO Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 153 - 153, Japanese


• 4Cp07 Highly-oriented co-immobilization of proteins on streptavidin-supported particles

  Matsumoto Takuya, Hata Yuto, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 196 - 196, Japanese


• 4Dp13 Benzoic acid fermentation from starch and cellulose using the polyketide synthesis pathway of Streptomyces maritimus

  Noda Shuhei, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 208 - 208, Japanese


• 麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位V_HH分泌生産系の構築

  吉栄俊秀, 青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

  2012, 日本農芸化学会大会講演要旨集(Web), 2012


• 1Kp11 IPA production from cellobiose by E. coli displayed BGL

  Souma Yuki, Inokuma Kentaro, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko, Hanai Taizo

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 69 - 69, Japanese


• 麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位V_HH分泌生産系の構築

  青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

  2011, 日本農芸化学会関西支部講演会講演要旨集, 472nd


• 麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位V_HHの生産

  青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

  2011, 日本生物工学会大会講演要旨集, 63rd


• バイオマスからのバイオ燃料生産に向けた戦略

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  Nov. 2010, バイオインダストリー, 11, 6-12, Japanese

  Introduction scientific journal


• Gene copy number and polyploidy on products formation in yeast

  Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(4) (4), 849 - 857, English

  [Refereed]

  Book review


• 次世代燃料・化成品原料製造に向けたバイオリファイナリー戦略

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  Jul. 2010, 生物工学, 88(7), 333-335, Japanese

  Introduction scientific journal


• Protein-protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling

  Jun Ishii, Nobuo Fukuda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2010, FEBS JOURNAL, 277(9) (9), 1982 - 1995, English

  [Refereed]

  Book review


• 2P-1106 Over-production of various secretory-form proteins in Streptomyces lividans

  NODA Shuhei, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 35 - 35, Japanese


• 3P-1082 Site-specific modification of Streptavidin using Sortase

  Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 78 - 78, Japanese


• 3P-2082 Development of non-invasive cancer cell killing method based on combination of titanium peroxiside nano-particle and low intensity X-ray irradiation

  KARASAKI Miki, MATSUI Kazusa, SATO Kazuyoshi, OHARA Satoshi, SASAKI Ryohei, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 168 - 168, Japanese


• 2P-2047 Direct ethanol production from cellulose by using cellulolytic enzymes expression optimized yeast strain

  YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 118 - 118, Japanese


• 3P-1011 Construction of the recombinant xylose fermentation yeast with high β-xylosidase activity from OC-2 triple marker strain as a platform

  Saitoh Satoshi, Tanaka Tsutomu, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 60 - 60, Japanese


• Applications of yeast cell-surface display in bio-refinery

  Akihiko Kondo, Tsutomu Tanaka, Tomohisa Hasunuma, Chiaki Ogino

  2010, Recent Patents on Biotechnology, 4(3) (3), 226 - 234, English

  [Refereed]

  Introduction scientific journal


• EP-P27 Site-specific streptavidin modification using sortase(Section VII Enzyme and Protein Engineering)

  Matsumoto Takuya, Sawamoto Shiori, Sakamoto Takayuki, Tanaka Tsutomu, Kondo Akihiko

  The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S108, English


• Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domain

  Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, English

  Summary international conference


• Efficient biodiesel production using whole-cell biocatalyst employing a lipase high expression system

  Tomohiro Takaya, Sriappareddy Tamalampudi, Kazunori Nakashima, Tsutomu Tanaka, Takayuki Bogaki, Junpei Wada, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S49 - S49, English

  Summary international conference


• Enzyme-mediated protein immobilization on particles

  Maiko Segawa, Kazusa Matsui, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S159 - S159, English

  Summary international conference


• Affibody displaying bionanocapsules for HER2 specific drug delivery

  Yuya Nishimura, Takuya Shishido, Hiroaki Mieda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S27 - S27, English

  Summary international conference


• Enzyme-mediated antibody-protein conjugation

  Takayuki Sakamoto, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S108 - S108, English

  Summary international conference


• Site-specific streptavidin modification using sortase

  Takuya Matsumoto, Shiori Sawamoto, Takayuki Sakamoto, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S108 - S108, English

  Summary international conference


• Efficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiae

  Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, English

  Summary international conference


• Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S61 - S62, English

  Summary international conference


• Expression and signaling analyses of human G protein-coupled receptor in yeast

  Yusuke Iguchi, Jun Ishii, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S164 - S164, English

  Summary international conference


• Enzyme-mediated site-specific protein modification

  Tsutomu Tanaka, Takayuki Sakamoto, Kanako Wakamura, Ryosuke Takase, Takuya Matsumoto, Shiori Sawamoto, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S96 - S97, English

  Summary international conference


• Site-specific protein modification with functional molecule using novel enzyme

  Ryosuke Takase, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S107 - S108, English

  Summary international conference


• Efficient D-lactic acid production from raw starch

  Satoru Shinkawa, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S47 - S48, English

  Summary international conference


• Efficient homo D-lactic acid production from xylose

  Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S48, English

  Summary international conference


• Enzymatic protein conjugation in vivo

  Kanako Wakamura, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S109 - S109, English

  Summary international conference


• Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipases

  Daisuke Adachi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S52, English

  Summary international conference


• Efficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genes

  Syunichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S51, English

  Summary international conference


• Efficient ethanol production from xylose by mated diploid Saccharomyces cerevisiae

  Hiroaki Suyama, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S49 - S49, English

  Summary international conference


• Engineering of endoglucanase expression system for Corynebacterium glutamicum

  Takeyuki Tsuchidate, Toshihiro Tateno, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S49, English

  Summary international conference


• Direct fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzyme

  Shuhei Yanase, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S52 - S52, English

  Summary international conference


• Functional analysis of mutant human somatostatin receptor using a yeast-based fluorescence reporter assay

  Shota Togawa, Jun Ishii, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S108 - S108, English

  Summary international conference


• Integrated and energy-saving biodiesel fuel production using fungus whole-cell biocatalyst

  Risa Koda, Kazunori Nakashima, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S51, English

  Summary international conference


• Antibody-immobilized TiO2 nanoparticles for cancer therapy

  Kazusa Matsui, Maiko Segawa, Tsutomu Tanaka, Akihiko Kondo, Chiaki Ogino

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S36 - S37, English

  Summary international conference


• 生体分子と無機材料を融合した中空バイオナノ粒子による新しいがん治療戦略

  近藤 昭彦, 荻野 千秋, 田中 勉

  Nov. 2009, 工業材料, 61-64, Japanese

  Introduction scientific journal


• Enzyme-assisted cell surface protein modification

  TANAKA Tsutomu, YAMAMOTO Teruyasu, NAGAMUNE Teruyuki

  バイオインダストリー協会, 01 Feb. 2009, Bioscience & industry, 67(2) (2), 65 - 67, Japanese


• 2La01 Efficient ethanol production from raw starch using novel yeast strain

  Yamakawa Syunichi, Yamada Ryosuke, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 170 - 170, Japanese


• 2La11 Development of novel cell-surface display system using porin in Corynebacterium glutamicum

  Hatada Kazuki, Tateno Toshihiro, Okai Naoko, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 172 - 172, Japanese


• 2Lp02 Efficient protein production in recombinant Aspergillus oryzae

  SAKAI Shoji, MATSUDA Tetsuya, ADACHI Takashi, HISADA Hiromoto, HATA Yoji, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 173 - 173, Japanese


• 特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用

  近藤 昭彦, 宍戸 卓矢, 田中 勉, 荻野 千秋

  30 Oct. 2008, バイオイメージング, 17(2) (2), 110 - 111, Japanese


• 細胞表層提示技術を用いた微生物の高機能化と有用物質生産

  TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  Aug. 2008, 月刊バイオインダストリー, Vol. 25, No. 8, pp. 13-20, Japanese

  Introduction scientific journal


• バイオリファイナリーが求める植物バイオマス

  OGINO Chiaki, TANAKA Tsutomu, FUKUDA Hideki, KONDO Akihiko

  Apr. 2008, 月刊バイオインダストリー, Vol. 25, No. 4, pp. 11-19, Japanese

  Introduction scientific journal


• 2Ep19 Efficient Production of Optically Pure D-Lactic Acid from Raw Starch

  ZHANG Qiao, OKANO Kenji, SHINKAWA Satoru, TANAKA Tsutomu, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 145 - 145, Japanese


• 2Aa08 Efficient ethanol production from raw starch by delta-integrant polyploid fusants

  YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 60 - 60, Japanese


• 2Aa07 Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpressing Lipases

  ADACHI Daisuke, NUMATA Takao, TANAKA Tsutomu, KONDO Akihiko, FUKUDA Hideki

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 60 - 60, Japanese


• 2Ap01 Biotin-displaying yeast for displaying functional molecule

  MASUNARI Shinsuke, TANAKA Tsutomu, ISHII Jun, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 63 - 63, Japanese


• 2Ap05 Fiber weight loss process using enzyme reaction and electron beam irradiation

  KURATA Seiichi, YAMAZAKI Hirotaka, TAKAYAMA Katsumi, ISHIGAKI Tomonori, BABA Toshiyuki, SEKI Takayuki, TANAKA Tsutomu, KONDO Akihiko, KURODA Koichi, UEDA Mitsuyoshi, SUYE Shin-ichiro

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 64 - 64, Japanese


• タンパク質間相互作用とシグナル伝達解析 −酵母細胞を用いたハイスループット技術の開発−

  Ishii Jun, Tanaka Tsutomu, Kondo Akihiko

  Jun. 2007, 未来材料, (6月号) (6月号), 40 - 45, Japanese

  Introduction commerce magazine


• 1D11-4 Efficient ethanol production process from raw starch by recombinant Saccharomyces cerevisiae

  YAMADA Ryosuke, BITO Yohei, TANAKA Tsutomu, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 97 - 97, Japanese


• 2H10-4 Development of a new technique for the site-specific incorporation of synthetic molecules into membrane-penetrating proteins on a live cell surface

  GODA Satoshi, TSUKIJI Shinya, TANAKA Tsutomu, NAGAMUNE Teruyuki

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 220 - 220, Japanese


• 2A10-1 A branched protein mediated electron transfer system

  HIRAKAWA Hidehiko, KAMIYA Noriho, TANAKA Tsutomu, NAGAMUNE Teruyuki

  日本生物工学会, 2004, 日本生物工学会大会講演要旨集, 16, 104 - 104, Japanese

• Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux.：Synthetic Biology, Methods in Molecular Biology

  MATSUMOTO Takuya, TANAKA Tsutomu, KONDO Akihiko

  Joint work, Humana Press, May 2018, English

  Scholarly book


• Sortase Aを用いたタンパク質配向固定化技術の開発

  MATSUMOTO TAKUYA, TANAKA TSUTOMU

  Joint work, シーエムシー出版, Apr. 2018, Japanese, 細胞・生体分子の固定化と機能発現 第3章

  Scholarly book


• 酵母菌・麹菌・乳酸菌の産業応用展開, 第III編 乳酸菌 第2章「乳酸菌の遺伝子操作技術の進展」

  岡野 憲司, TANAKA TSUTOMU, 本田 孝祐, KONDO AKIHIKO

  Joint work, シーエムシー出版, Jan. 2018, Japanese

  Scholarly book


• Lactic Acid

  Kenji Okano, Tsutomu Tanaka, Akihiko Kondo

  Joint work, Wley, Apr. 2014, English

  Scholarly book


• 遺伝子工学

  KONDO AKIHIKO, 田中 勉

  Joint work, 化学同人, Jun. 2012, Japanese

  Scholarly book


• エコバイオリファイナリー

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  Joint work, シーエムシー出版, Dec. 2010, Japanese

  Scholarly book


• シングルセル解析の最前線 （第３章 細胞内生体分子群の実測定量解析，第4節 「フローサイトメトリーとGFPレポーターによるG蛋白質シグナルのシングルセル解析」）

  石井 純, 田中 勉, 荻野 千秋, 近藤 昭彦

  Joint work, シーエムシー出版, Mar. 2010, Japanese

  Scholarly book


• 第二世代バイオ燃料の開発と応用展開

  近藤 昭彦, 福田 秀樹, 荻野 千秋, 田中 勉, 蓮沼 誠久, 原田 和生, 福崎 英一郎

  Joint work, シーエムシー出版, Apr. 2009, Japanese

  Scholarly book


• 微生物によるものづくり

  植田 充実, 芝崎 誠司, 荻野 千秋, 近藤 昭彦, 谷野 孝徳, 田中 勉, 福田 秀樹

  Single work, シーエムシー出版, Jun. 2008, Japanese

  Scholarly book

• 分裂酵母の代謝改変によるβ-カロテン生産

  伊藤 美紀, 田上 満里奈, 田中 勉, 近藤 昭彦


• コリネ菌におけるシキミ酸経路の強化

  佐藤 直樹, 田中 勉, 近藤 昭彦

  化学工学会第85年会


• 大腸菌を用いたメバロン酸生産技術の開発

  里和 大地, 田中 勉, 近藤 昭彦

  化学工学会第85年会


• "Parallel Metabolic Pathway Engineering" of Escherichia coli for Shikimate Pathway Derivative Production from Glucose-Xylose Co-substrate

  藤原 良介, 野田 修平, 田中 勉, 近藤 昭彦

  化学工学会第85年会


• Parallel Metabolic Pathway Engineeringを用いた大腸菌による高収率物質生産

  藤原良介, 野田修平, 田中 勉, 近藤昭彦

  酵素工学研究会第８２回講演会, Nov. 2019, 酵素工学研究会, 東京工業大学, Domestic conference


• コリネ菌を用いたバイオマスからの脂肪酸生産技術の開発

  奥 香奈, 佐藤直樹, 田中 勉, 近藤昭彦

  酵素工学研究会第８２回講演会, Nov. 2019, 酵素工学研究会, 東京工業大学, Domestic conference


• 大腸菌を用いたイソペンテノール生産のための代謝改変

  内尾匠吾, 里和大地, 田中 勉, 近藤昭彦

  酵素工学研究会第８２回講演会, Nov. 2019, 酵素工学研究会, 東京工業大学, Domestic conference


• 分裂酵母の代謝改変によるβ-カロテン生産

  伊藤美紀, 田上満里奈, 田中 勉, 近藤昭彦

  酵素工学研究会第８２回講演会, Nov. 2019, 酵素工学研究会, 東京工業大学, Domestic conference


• メバロン酸生産 のための新規ターゲット遺伝子の探索

  里和大地, 藤原良介, 田中 勉, 近藤昭彦

  酵素工学研究会第８２回講演会, Nov. 2019, 酵素工学研究会, 東京工業大学, Domestic conference


• コリネ菌におけるシキミ酸経路の強化

  佐藤直樹, 田中 勉, 近藤昭彦

  酵素工学研究会第８２回講演会, Nov. 2019, 酵素工学研究会, 東京工業大学, Domestic conference


• The effect of single gene knockout on mevalonate production by Escherichia coli

  Takuya MATSUMOTO, Naoki HAMADA, Tsutomu TANAKA, Hiroyasu OGINO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, APCChE, Sapporo Convention Center

  Poster presentation


• High yield bioproduction of shikimate pathway derivatives by unitization and combination of purpose-specific metabolic pathways

  Ryosuke FUJIWARA, Shuhei NODA, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• Beta-carotene Production Using Genome Edited Schizosaccharomyces pombe

  Miki ITO, Marina TANOUE, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• Enhancement of fatty acid productivity using metabolically engineered Corynebacterium glutamicum

  Kana OKU, Naoki SATO, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• Metabolic engineering of Escherichia coli for isopentenol production

  Shogo UCHIO, Daichi SATOWA, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• Metabolic engineering of Escherichia coli for production of p-aminobenzoic acid

  Tatsuya TAKAHASHI, Ryosuke FUJIWARA, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• Production of shikimic acid using metabolically engineered Corynebacterium glutamicum

  Naoki SATO, Rena MATSUURA, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• 遺伝子組換え乳酸菌によるコーンコブを原料としたD-乳酸の高濃度・高収率生産

  内藤 秋花, 堀内 淳一, 熊田 陽一, 岡野 憲司, KONDO AKIHIKO, TANAKA TSUTOMU

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• Metabolic engineering to improve 1,5-diaminopentane production from cellobiose using BGL-secreting Corynebacterium glutamicum

  Rena MATSUURA, Tsutomu TANAKA, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Jul. 2019, English, APCChE, Sapporo Convention Center, International conference

  Poster presentation


• The effect of gene knockout on mevalonate production orelectricity generation by Escherichia coli

  Takuya Matsumoto, Naoki Hamada, Takuto Matsui, Tsutomu Tanaka, Hiroyasu Ogino

  14th International symposium on Biocatalysis and Biotransformations (Biotrans 2019), Jul. 2019, English, University of Groningen, the MartiniPlaza, International conference

  Poster presentation


• Metabolic engineering of Escherichia coli for production of mevalonate

  Daichi Satowa, Ryosuke Fujiwara, Tsutomu Tanaka, Akihiko Kondo

  14th International symposium on Biocatalysis and Biotransformations (Biotrans 2019), Jul. 2019, English, University of Groningen, the MartiniPlaza, International conference

  Poster presentation


• High yield bioproduction of shikimate pathway derivatives by"Parallel Metabolic Design"

  Ryosuke Fujiwara, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  14th International symposium on Biocatalysis and Biotransformations (Biotrans 2019), Jul. 2019, University of Groningen, the MartiniPlaza


• High yield production of muconic acid from glucose by supplementing the carbon source from xylose for cell growth.

  Ryosuke Fujiwara, Tsutomu Tanaka, Shuhei Noda, Akihiko Kondo

  Symposium on Biotechnology for Fuels and Chemicals (SBFC), Apr. 2019, Society for Industrial Microbiology and Biotechnology, Hyatt Regency Seattle


• Enhancing 3-hydroxypropionic acid production in combination with cell surface display and metabolic engineering of S. pombe

  Tsutom Tanaka, Akihiko Kondo

  Symposium on Biotechnology for Fuels and Chemicals (SBFC), Apr. 2019, Society for Industrial Microbiology and Biotechnology, Hyatt Regency Seattle


• コーンコブを原料とした代謝改変乳酸菌による高濃度高収率D-乳酸生産

  内藤 秋花, 堀内 淳一, 熊田 陽一, 岡野 憲司, KONDO AKIHIKO, TANAKA TSUTOMU

  化学工学会第84年会, Mar. 2019, Japanese, 芝浦工業大学 豊洲キャンパス, Domestic conference

  Poster presentation


• 酵素反応によりゲルネットワークを精密に合成した金ナノ粒子／DNAハイブリッドゲル

  Maruyama Tatsuo, 永口侑香, 加藤智晴, Tanaka Tsutomu

  第67回高分子討論会, Sep. 2018, Japanese, 公益社団法人高分子学会, 札幌, Domestic conference

  Oral presentation


• 糖の使い分けによる高収率物質生産技術の開発

  藤原 良介, 野田 修平, TANAKA TSUTOMU, KONDO AKIHIKO

  化学工学会第50回秋季大会, Sep. 2018, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference

  Poster presentation


• 大腸菌を用いたセロビオースからのメバロン酸生産技術の開発

  里和 大地, 藤原 良介, TANAKA TSUTOMU, KONDO AKIHIKO

  化学工学会第50回秋季大会, Sep. 2018, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference

  Poster presentation


• 遺伝子組換え乳酸菌によるコーンコブを原料とした高収率D-乳酸生産

  内藤 秋花, 堀内 淳一, 熊田 陽一, 岡野 憲司, KONDO AKIHIKO, TANAKA TSUTOMU

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• コリネ菌を用いたセロビオースからのシキミ酸生産技術の開発

  佐藤 直樹, TANAKA TSUTOMU, 松浦 礼奈, KONDO AKIHIKO

  化学工学会第50回秋季大会, Sep. 2018, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference

  Poster presentation


• Target-AID を利用したゲノム編集による高収率ブタ ノール発酵性クロストリジウム属微生物の育種

  向山正治, 市毛栄太, 田中 勉, 西田敬二, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• n-ブタノールを経由した大腸菌利用によるn-ブチルアミン生産技術の開発

  森 裕貴, MATSUMOTO TAKUYA, TANAKA TSUTOMU, KONDO AKIHIKO

  化学工学会第50回秋季大会, Sep. 2018, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference

  Poster presentation


• Metabolic engineering of Escherichia coli for production of shikimate pathway derivatives

  Ryosuke Fujiwara, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Corynebacterium glutamicumを用いたセロビオースからのカダベリン生産技術の開発

  松浦 礼奈, 岸田 真裕美, TANAKA TSUTOMU, KONDO AKIHIKO

  化学工学会第50回秋季大会, Sep. 2018, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference

  Poster presentation


• Production of shikimic acid from cellobiose using Corynebacterium glutamicum

  SATO NAOKI, TANAKA TSUTOMU, KONDO AKIHIKO

  The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering, Jun. 2018, English, 京都大学, International conference

  Poster presentation


• Production of n-butylamine in Escherichia coli by transaminase

  MORI YUKI, MATSUMOTO TAKUYA, TANAKA TSUTOMU, KONDO AKIHIKO

  The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering, Jun. 2018, English, 京都大学, International conference

  Poster presentation


• Metabolic engineering of S. pombe via CRISPR-Cas9 genome editing for 3-hydroxyprpininc acid production from glucose and cellobiose

  TANAKA TSUTOMU, TAKAYAMA SEIYA, KONDO AKIHIKO

  The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering, Jun. 2018, English, 京都大学, International conference

  Poster presentation


• Metabolic engineering of Escherichia coli for production of mevalonate from cellobiose

  SATOWA DAICHI, FUJIWARA RYOSUKE, TANAKA TSUTOMU, KONDO AKIHIKO

  The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering, Jun. 2018, English, 京都大学, International conference

  Poster presentation


• Metabolic design of Escherichia coli for production of shikimate pathway derivatives

  FUJIWARA RYOSUKE, NODA SHUHEI, TANAKA TSUTOMU, KONDO AKIHIKO

  The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering, Jun. 2018, English, 京都大学, International conference

  Poster presentation


• Cadaverine production from cellobiose using Corynebacterium glutamicum

  MATSUURA RENA, TANAKA TSUTOMU, KONDO AKIHIKO

  The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering, Jun. 2018, English, 京都大学, International conference

  Poster presentation


• 分裂酵母を用いた有機酸生産技術の開発

  TANAKA TSUTOMU, 高山 征也, 尾崎 愛子, KONDO AKIHIKO

  化学工学会第83年会, Mar. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 代謝デザイン大腸菌を利用したムコン酸発酵生産

  藤原 良介, 野田 修平, TANAKA TSUTOMU, KONDO AKIHIKO

  化学工学会第83年会, Mar. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Sortaggingを用いた大腸菌代謝酵素の連結

  MATSUMOTO TAKUYA, TANAKA TSUTOMU, KONDO AKIHIKO

  化学工学会第83年会, Mar. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 分裂酵母の代謝改変株を用いた3-ヒドロキシプロピオン生産

  高山 征也, 山口 理絵, TANAKA TSUTOMU, Akihiko Kondo

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• 代謝デザイン大腸菌を利用したムコン酸発酵生産

  藤原 良介, 野田 修平, TANAKA TSUTOMU, KONDO AKIHIKO

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• 酵素カスケード反応を用いたヘキサメチレンジアミン生産

  入村 高史, MATSUMOTO TAKUYA, TANAKA TSUTOMU, KONDO AKIHIKO

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• トランスアミナーゼを用いた菌体内n-ブチルアミン生産技術の開発

  森 裕貴, MATSUMOTO TAKUYA, TANAKA TSUTOMU, Akihiko Kondo

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• コリネ菌を用いたセルロース系バイオマスからのカダベリン生産技術の開発

  松浦 礼奈, TANAKA TSUTOMU, Akihiko Kondo

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• グルカル酸生産を志向した大腸菌の代謝改変

  中西 將裕, MATSUMOTO TAKUYA, TANAKA TSUTOMU, Akihiko Kondo

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• Sortaggingを用いた代謝チャネリング技術の開発

  MATSUMOTO TAKUYA, TANAKA TSUTOMU, KONDO AKIHIKO

  酵素工学研究会第７８回講演会, Oct. 2017, Japanese, カレッジプラザ, Domestic conference

  Poster presentation


• 分裂酵母の代謝改変株を用いた3-ヒドロキシプロピオン酸生産

  高山 征也, 小西 理絵, TANAKA TSUTOMU, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 早稲田大学 西早稲田キャンパス, Domestic conference

  Oral presentation


• 大腸菌を用いたムコン酸の高効率発酵生産

  藤原 良介, 野田 修平, TANAKA TSUTOMU, KONDO AKIHIKO

  第69回日本生物工学会大会, Sep. 2017, Japanese, 早稲田大学 西早稲田キャンパス, Domestic conference

  Poster presentation


• 酵素ステープラーを用いた代謝酵素の連結による代謝改変技術

  MATSUMOTO TAKUYA, TANAKA TSUTOMU, Akihiko Kondo

  化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋大学東山キャンパス, Domestic conference

  Poster presentation


• 酵素カスケード反応を用いたヘキサメチレンジアミン生産

  入村 高史, MATSUMOTO TAKUYA, TANAKA TSUTOMU, Akihiko Kondo

  化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋大学東山キャンパス, Domestic conference

  Poster presentation


• トランスアミナーゼを用いた大腸菌内におけるブチルアミン生産

  森 裕貴, MATSUMOTO TAKUYA, TANAKA TSUTOMU, Akihiko Kondo

  化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋大学東山キャンパス, Domestic conference

  Poster presentation


• Target-AID を利用したゲノム編集による高収率ブタ ノール発酵性クロストリジウム属微生物の育種

  向山正治, 市毛栄太, 田中 勉, 西田敬二, 近藤 昭彦

  第69回日本生物工学会大会, Sep. 2017, Japanese, 早稲田大学 西早稲田キャンパス, Domestic conference

  Oral presentation


• Corynebacterium glutamicumを用いたセルロース系バイオマスからのカダベリン生産技術の開発

  松浦 礼奈, 岸田 真裕美, 平田 有希, 田中 勉, KONDO AKIHIKO

  第69回日本生物工学会大会, Sep. 2017, Japanese, 早稲田大学 西早稲田キャンパス, Domestic conference

  Poster presentation


• 細胞表層工学と代謝工学を用いた物質生産

  TANAKA TSUTOMU

  合成生物工学シンポジウム, Aug. 2017, Japanese, 神戸大学百年記念館六甲ホール, Domestic conference

  Oral presentation


• 細胞表層工学と合成代謝経路による物質生産

  TANAKA TSUTOMU

  バイオ工学シンポジウム, Jul. 2017, Japanese, 大和屋本店 愛媛大学, Domestic conference

  Oral presentation


• Metabolic engineering of S. pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose

  Tsutomu Tanaka

  SB7.0 The Seventh International Meeting on Synethetic Biology, Jun. 2017, English, University Cultural Center, International conference

  Oral presentation


• Target-AID を利用したゲノム編集による高収率ブタ ノール発酵性クロストリジウム属微生物の育種

  向山正治, 市毛栄太, 田中 勉, 西田敬二, 近藤 昭彦

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都女子大学, Domestic conference

  Oral presentation


• Metabolic engineering for putrescine production from cellobiose by betaglucosidase displaying E. coli

  TANAKA TSUTOMU

  the 17th International Biotechnology Symposium and Exhibition (IBS 2016), Oct. 2016, English, IBS, Melbourne, Australia, International conference

  Oral presentation


• 分裂酵母におけるマロニルCoAを介した有機酸生合成経路の構築

  尾崎 愛子, 山口 理絵, Tsutom Tanaka, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• 酵素カスケード反応を用いたヘキサメチレンジアミン生産

  入村 高史, 松本 拓也, Tsutom Tanaka, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• バイオ燃料電池への応用を志向したストレプトアビジンハイドロゲルの作製

  五十川 由季, 松本 拓也, Tsutom Tanaka, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• Sortase A を用いた大腸菌体内での代謝酵素の連結とその影響

  松本 拓也, Tsutom Tanaka, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• Sortase Aを用いた大腸菌体内での代謝酵素の連結とその影響

  松本 拓也, Tsutom Tanaka, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• CRISPR Cas9 systemによる遺伝子組み換えSchizosaccharomyces pombeを用いた有機酸生産

  高山 征也, 尾崎 愛子, 山口 理絵, Tsutom Tanaka, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• Corynebacterium glutamicum を用いたヘミセルロース系バイオマスからのカダベリン生産

  今尾 健太, 山口 理絵, Tsutom Tanaka, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• BGL提示大腸菌に適した代謝改変戦略を用いたセロビオースからのプトレシン生産

  Tsutom Tanaka, 西川 弘樹, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• Putrescine production from cellobiose by cell surface- and metabolically-engineered E. coli

  Tsutom Tanaka, Hiroki Nishikawa, Naoki Ikeda, KONDO Akihiko

  17th European Congress on Biotechnology (ECB2016), Jul. 2016, English, ECB2016, Kraków; Poland, International conference

  Oral presentation


• Metabolic enzyme ligation by sortagging

  Matsumoto Takuya, Tsutom Tanaka, KONDO Akihiko

  17th European Congress on Biotechnology (ECB2016), Jul. 2016, English, ECB2016, Kraków; Poland, International conference

  Oral presentation


• 異種酵素からなる一次元酵素集合体の設計と協奏効果の検討

  TANAKA TSUTOMU, KAWASHIMA HIROKI, MORI YUTAROU, NAKAZAWA HIKARU, UMEDU MITSUO, KAMIYA NORIHO

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• β-グルコシダーゼ提示大腸菌を用いたセロビオースからのプトレシン生産

  Nishikawa Hiroki, Tanaka Tsutomu, Kondo Akihiko

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• ポリメラーゼ連鎖反応を利用したDNA―金ナノ粒子ハイブリッドゲルの作製と特性評価

  永口侑香, 加藤智晴, Tanaka Tsutomu, Maruyama Tatsuo

  化学工学会第81年会本部大会, Mar. 2016, Japanese, 化学工学会, 福岡市, Domestic conference

  Poster presentation


• SorataseAを用いた大腸菌体内での代謝酵素連結技術の開発

  Furuta K, Matsumoto Takuya, Tanaka Tsutomu, Kondo Akihiko

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• Streptavidin Hydrogel as a Scaffold for Protein Assembly

  Isogawa Yuki, Tanaka Tsutomu, Kondou Akihiko, Matsumoto Takuya

  Protein & Antibody Engineering Summit (PEGS EUROPE), Nov. 2015, English, International conference

  Oral presentation


• C-terminal-oriented immobilization of enzymes using sortase A-mediated technique

  Hata Yuto, Matsumoto Takuya, Kondo Akihiko, Tanaka Tsutomu

  Protein & Antibody Engineering Summit (PEGS EUROPE), Nov. 2015, English, International conference

  Oral presentation


• 効率的なブタノール生産に向けた代謝チャネリング技術の開発

  Furuta, Matsumoto Takuya, Tanaka Tsutomu, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• Sortase Aを用いた酵素配向固定化微粒子の機能評価

  Matsumoto Takuya, Tanaka Tsutomu, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• Production of 2,3-butanediol from cellobiose using Baccillus subtilis

  Tanimura Kousuke, Takashima Shingo, Tanaka Tsutomu, Kondo Akihiko

  The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• Direct cadaverine production from cellobiose using β-gulucosidase displaying Esherichia coil

  Tanaka Tsutomu, Ikeda Noaki, Nakano Mariko, Kondou Akihiko

  The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• C-terminal-oriented immobilization of enzymes using sortase A-mediated technique

  Matsumoto Takuya, Tanaka Tsutomu, Kondou Akihiko

  The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• Corynebacterium glutamicumを用いたバイオマスからのカダベリン生産

  Imao Kenta, Segawa Syota, Tanaka Tsutomu, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• 酵素固定化足場としてのストレプトアビジンハイドロゲルの作成

  Isokawa Yuki, Matsumoto Takuya, Tanaka Tsutomu, Kondou Akihiko

  化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• 酵素ステープラーを用いた大腸菌体内での代謝酵素の連結

  Matsumoto Takuya, Tanaka Tsutomu, Kondou Akihiko

  化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• タンパク質ｰリガンド相互作用を介した一次元セルラーゼ集合体の構築と触媒特性

  TANAKA TSUTOMU, KAWASHIMA HIROKI, MORI YUTAROU, NAKAZAWA HIKARU, UMEDU MITSUO, KAMIYA NORIHO

  化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• PCR法を用いた金ナノ粒子を架橋点とするDNAゲルの構築

  永口侑香, 加藤智晴, Tanaka Tsutomu, Maruyama Tatsuo

  第64回高分子討論会, Sep. 2015, Japanese, 高分子学会, 仙台市, Domestic conference

  Poster presentation


• PCR増幅による金ナノ粒子を架橋点としたDNAゲルの形成

  永口侑香, 加藤智晴, Tanaka Tsutomu, Maruyama Tatsuo

  化学工学会第47回秋季大会, Sep. 2015, Japanese, 化学工学会, 札幌市, Domestic conference

  Poster presentation


• CRISPR Cas9 system を用いて代謝改変を行った分裂酵母による有機酸生産技術の開発

  Ozaki Aiko, Yamaguchi Rie, Kondou Akihiko, Tanaka Tsutomu

  化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• ポリメラーゼ連鎖反応を用いた金ナノ粒子を架橋点とするDNAゲルの形成

  永口侑香, 加藤智晴, Tanaka Tsutomu, Maruyama Tatsuo

  第61回高分子研究発表会（神戸）, Jul. 2015, Japanese, 高分子学会, 神戸市, Domestic conference

  Poster presentation


• Hydrogel composed of DNA-gold nanoparticle network prepared by polymerase chain reaction

  永口侑香, 加藤智晴, Tanaka Tsutomu, Maruyama Tatsuo

  4th International Frontiers in Polymer Science, May 2015, English, Elsevier, Riva del Garda, International conference

  Poster presentation


• 線菌のタンパク質分泌機構を用いた機能性ストレプトアビジン生産

  田中 陽祐, 野田 修平, 田中 勉, KONDO AKIHIKO

  化学工学会第80年会, Mar. 2015, Japanese, 化学工学会, 江東区, Domestic conference

  Poster presentation


• 枯草菌によるセロビオースからの2,3-ブタンジオール生産

  高島 慎吾, 谷村 幸亮, 田中 勉, KONDO AKIHIKO

  化学工学会第80年会, Mar. 2015, Japanese, 化学工学会, 江東区, Domestic conference

  Poster presentation


• Sortase Aを用いた酵素の配向固定化とその評価

  松本 拓也, 秦 悠斗, 田中 勉, KONDO AKIHIKO

  化学工学会第80年会, Mar. 2015, Japanese, 化学工学会, 江東区, Domestic conference

  Oral presentation


• 大腸菌を用いたオリゴ糖からのカダべリン生産系の構築

  池田 直樹, 田中 勉, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 多機能性ベータグルコシダーゼを用いたセルロース/ ヘミセルロース様基質の糖化

  松本 拓也, 秦 悠斗, 田中 勉, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 多機能性ベータグルコシダーゼの機能評価とその応用

  松本 拓也, 秦 悠斗, 田中 勉, KONDO AKIHIKO

  化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡市, Domestic conference

  Oral presentation


• 線菌を用いたバイオマス資源からのスチレン系化合物生産

  藤原 良介, 野田 修平, 河井 禎文, 田中 勉, KONDO AKIHIKO

  化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡市, Domestic conference

  Poster presentation


• 新規セルラーゼ-ポリマーハイブリッドによる結晶性セルロースの加水分解挙動

  佐伯 貴史, 若林 里衣, 森 裕太郎, 八尋 謙介, 一瀬 博文, 田中 勉

  化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡市, Domestic conference

  Poster presentation


• 高収率・高生産型クロストリジウム属ホモブタノール生産菌の解析

  向山 正治, 中之庄 正弘, 市毛 栄太, 土橋 幸生, 中山 俊一, 田中 勉, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 枯草菌によるバイオマス由来炭素源からの2,3-butanediol生産

  谷村 幸亮, 高島 慎吾, 田中 勉, KONDO AKIHIKO

  化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡市, Domestic conference

  Oral presentation


• 化学的前処理バガスの酵素分解におけるキシラナーゼの協奏効果の検討

  高杉 優作, 森 裕太郎, JIA L, LOPES Goncalves G. A, 引野 幸枝, 田中 勉

  化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡市, Domestic conference

  Poster presentation


• β- グルコシターゼ提示分裂酵母を用いた乳酸生産

  小川 晃右, 松本 紗世子, 田中 勉, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• Sortase A を用いた酵素配向固定化粒子の作製

  秦 悠斗, 松本 拓也, 田中 勉, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• BGL提示Corynebacterium glutamicumを用いたセロビオースからのジアミン生産

  瀬川 将太, 足立 典子, 田中 勉, KONDO AKIHIKO

  化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡市, Domestic conference

  Poster presentation


• Co-assimilation of cellobiose and xylooligosaccharides using E. coli displaying both beta-glucosidase and beta-xylosidase on its cell surface

  TANAKA Tsutomu

  EUROPEAN BIOTECHNOLOGY CONGRESS 2014, May 2014, English, European Biotechnology Thematic Network Assosiation, Lecce, Italy, International conference

  Poster presentation


• βグルコシダーゼ提示大腸菌を用いたセロビオースからのカダべリン生産

  池田 直樹, 田中 勉

  化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conference

  Poster presentation


• Avidin-biotin相互作用による新規自己集合性人工セルロソームの構築

  森 裕太郎, 中澤 光, 田中 勉

  化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conference

  Poster presentation


• Creation of Escherichia coli Displaying both β-Glucosidase and β-Xylosidase on Its Cell Surface

  TANAKA Tsutomu

  Korea-Japan Smart Biodesign Workshop:Technology exchange for green biotechnology, Jan. 2014, English, 東北大学, 仙台市, International conference

  Oral presentation


• 糖の輸送能力強化によるエクトイン高生産Halomonas elongata の創製

  谷村 幸亮, 仲山 英樹, 田中 勉

  酵素工学研究会 第70回講演会, Oct. 2013, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• 放線菌を用いたセルロースからのp-クマル酸生産

  河井 禎文, 野田 修平, 田中 勉

  化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference

  Poster presentation


• 放線菌を用いたp-ヒドロキシスチレン生産

  藤原 良介, 野田 修平, 河井 禎文, 田中 勉

  化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference

  Poster presentation


• β-グルコシダーゼ表層提示 Corynebacterium glutamicumを用いたセロビオースからのリジン生産

  足立 典子, 田中 勉

  化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference

  Poster presentation


• β-グルコシダーゼ提示大腸菌を用いたプトレシン生産経路の構築

  池田 直樹, 宮本 茉里, 田中 勉

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• セルロース系バイオマスの酵素分解における協奏効果に関する基礎検討

  高杉 優作, 森 裕太郎, 一瀬 博文, 田中 勉

  化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference

  Poster presentation


• DNA足場上への異種セルラーゼ提示による人工セルロソームの設計

  森 裕太郎, 小笹 詩織, 田中 勉

  化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference

  Poster presentation


• Co-assimilation of cellobiose and xylooligosaccharides using E. coli displaying both beta-glucosidase and beta-xylosidase on its cell surface

  TANAKA Tsutomu

  Enzym Engineering XXII, Sep. 2013, English, 酵素工学研究会, 富山市, International conference

  Poster presentation


• Co-assimilation of cellobiose and xylooligosaccharides using E. coli displaying both b-glucosidase andb-xylosidase on its cell surface

  TANAKA Tsutomu

  The 19th Symposium of Young Asian Biochemical Engineers' Communyty, Aug. 2013, English, 生物工学会, Xinjiang,China, International conference

  Oral presentation


• Starchy and Cellulosic Biomass-Powered Enzymatic Biofuel Cells based on Degrading and Oxidizing Enzymes Multi-Immobilized Bioanodes

  MATSUMOTO Takuya, TANAKA Tsutomu, KONDO Akihiko

  9th European Congress of chemical engineering, Apr. 2013, English, European Society of Biochemical Engineering Science, The Hague, Netherland, International conference

  Poster presentation


• Co-Assimilation of Celloorigosaccharide and Xyloorigosaccharide Using E.coli Displaying both Beta-Glucosidase

  TANAKA Tsutomu, HIRATA Yuuki, KAWABATA Hitomi, KONDO Akihiko

  9th European Congress of chemical engineering, Apr. 2013, English, European Society of Biochemical Engineering Science, The Hague, Netherland, International conference

  Poster presentation


• 乳酸菌を用いたオリゴ糖からのD-乳酸生産技術の開発

  小郷 裕也, 篠原 正隆, 田中 勉, KONDO AKIHIKO

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• β-グルコシダーゼ発現Corynebacterium glutamicumを用いたセロビオースからのリジン生産

  足立 典子, 田中 勉, KONDO AKIHIKO

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• βグルコシダーゼ提示大腸菌を用いたプトレシン生産経路の構築

  池田 直樹, 宮本 茉里, 田中 勉, KONDO AKIHIKO

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• セロビオース資化性分裂酵母を用いた3-ヒドロキシプロピオン酸生産技術の開発

  山田 真璃, 松本 紗世子, 小川 晃右, 田中 勉, KONDO AKIHIKO

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• Sortase A を用いた酵素配向固定化微粒子の作製

  秦 悠斗, 松本 拓也, 田中 勉, KONDO AKIHIKO

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• 放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索

  宮崎 貴也, 野田 修平, 田中 勉, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• 糖質バイオマスから多様なポリエステルを生産するコリネ菌微生物工場の開発

  宋 育陽, 松本 謙一郎, 大井 俊彦, 田中 勉, KONDO AKIHIKO

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• 代謝改変によるエクトイン高生産Halomonas elongata の創製

  谷村 幸亮, 仲山 英樹, 田中 勉, KONDO AKIHIKO

  酵素工学研究会第６８回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• β- グルコシダーゼ発現Corynebacterium glutamicum を用いたセロビオースからのリジン生産

  足立 典子, 小野 尚子, 平田 有希, 高橋 千尋, OKAI NAOKO, 田中 勉, KONDO AKIHIKO

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• バイオマス直接利用型電極の開発

  嶋田 翔太, 山本 一寛, 松本 拓也, 田中 勉, KONDO AKIHIKO

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• ストレプトアビジンを介したタンパク質の高配向同時固定化技術の開発

  松本 拓也, 秦 悠斗, 田中 勉, KONDO AKIHIKO

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

  野田 修平, 田中 勉, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

  野田 修平, 田中 勉, OGINO CHIAKI

  酵素工学研究会第６８回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• Sortase A を用いた高配向なタンパク質固定化技術の開発

  松本 拓也, 田中 勉, KONDO AKIHIKO

  酵素工学研究会第６８回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• Halomonas elongata を用いたバイオマスからのエクトイン生産技術の開発

  谷村 幸亮, 仲山 英樹, 田中 勉, KONDO AKIHIKO

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• 目的タンパク質のみを特異的に認識する機能性高分子多孔膜の開発

  石岡 佑美, 本庄 崇文, 田中 勉, MATSUYAMA HIDETO, MARUYAMA TATSUO

  化学工学会 第44回秋季大会, Sep. 2012, Japanese, 化学工学会, 東北大学, Domestic conference

  Poster presentation


• 目的タンパク質のみを特異的に認識する機能性高分子

  石岡 佑美, 本庄 崇文, 田中 勉, MATSUYAMA HIDETO, Maruyama Tatsuo

  化学工学会 第44回秋季大会, Sep. 2012, Japanese, 高分子学会, 仙台市, Domestic conference

  Poster presentation


• 木質系バイオマス直接利用型電極の開発

  嶋田 翔太, 山本 一寛, 松本 拓也, 田中 勉, KONDO AKIHIKO

  化学工学会第44回秋季大会, Sep. 2012, Japanese, 化学工学会, 仙台市, Domestic conference

  Oral presentation


• バイオマス資化性大腸菌を用いたグルカル酸生産技術の開発

  宮本 茉里, 田中 勉, KONDO AKIHIKO

  化学工学会第44回秋季大会, Sep. 2012, Japanese, 化学工学会, 仙台市, Domestic conference

  Oral presentation


• 特定タンパク質選択性を有する高分子多孔膜の開発

  本庄 崇文, 石岡 佑美, Maruyama Tatsuo, 田中 勉, MATSUYAMA HIDETO

  第58回高分子研究発表会（神戸）, Jul. 2012, Japanese, 高分子学会, 神戸市, Domestic conference

  Poster presentation


• ラジカルを発生する金属酸化物粒子に対するXAFSによる非破壊状態分析

  沼子 千弥, 佐藤 和好, 高見 誠一, 梅津 光央, 田中 勉, OGINO CHIAKI

  第72回分析化学討論会, May 2012, Japanese, 日本分生化学会, 鹿児島市, Domestic conference

  Oral presentation


• 麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築

  吉栄 俊秀, 青木 淳一, 田淵 聡一郎, 岡崎 文美, 荻野 千秋, 田中 勉, 久田 博元, 秦 洋二, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• セルロースからの高効率一段階エタノール発酵を可能にする高機能酵母の創製

  中谷 侑紀, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌を用いたセルロースからの有用タンパク質生産

  河井 禎文, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

  野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• Streptaivdinを用いた部位特異的な異種タンパク質同時固定化技術の開発

  松本 拓也, 田中 勉, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• Halomonas elongataを用いたバイオマスからの高効率エクトイン生産プロセスの開発

  谷村 幸亮, 仲山 英樹, 田中 勉, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• Creation of cellooligosaccharide-assimilating E. coli by displaying active BGL on the cell surface using novel anchor protein

  TANAKA Tsutomu

  The 2nd i-BioP Asian Symposium, Dec. 2011, English, EBRC, Gyeongbuk、Korea, International conference

  Oral presentation


• 組換え麹菌生産セルラーゼを用いた広葉樹クラフトパルプの酵素糖化

  吉栄 俊秀, 酒井 翔司, 岡﨑 文美, 荻野 千秋, 田中 勉, 久田 博元, 萩原 伸哉, 秦 洋二, 近藤 昭彦

  第11回糸状菌分子生物学コンファレンス(2011), Nov. 2011, Japanese, 糸状菌分子生物学研究会, 文京区, Domestic conference

  Poster presentation


• ラクダ由来一本鎖抗体可変部位 VHH の麹菌 Aspergillus oryzae による分泌生産

  青木 淳一, 田淵 聡一郎, 岡﨑 文美, 荻野 千秋, 田中 勉, 久田 博元, 秦 洋二, 近藤 昭彦

  第11回糸状菌分子生物学コンファレンス(2011), Nov. 2011, Japanese, 糸状菌分子生物学研究会, 文京区, Domestic conference

  Poster presentation


• Halomonas elongata を用いたバイオマス由来C5糖からの高効率エクトイン生産プロセスの開発

  谷村 幸亮, 仲山 英樹, 田中 勉, 近藤 昭彦

  極限環境生物学会2011年度(第12回)年会, Nov. 2011, Japanese, 極限環境生物学会, 長崎市, Domestic conference

  Poster presentation


• 放線菌が有する炭素源資化能力を生かしたタンパク質の大量分泌生産

  宮崎 貴也, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• 高効率バイオエタノール製造技術開発- SSCF(同時糖化並行複発酵)のための糖化発酵技術-

  保谷 典子, 徳原 渡, 仲田 利樹, 則武 義幸, 田中 勉, 近藤 昭彦, 大西 徹, 金川 章孝

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• 酵素を用いたタンパク質高機能化技術の開発

  田中 勉

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  [Invited]

  Invited oral presentation


• βグルコシダーゼを表層提示したセロオリゴ糖資化性大腸菌の創製

  田中 勉, 川畑 瞳, 平田 有希, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• バイオマス同時糖化酸化型電極の開発

  山本 一寛, 松本 拓也, 田中 勉, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• セルラーゼ発現最適化二倍体酵母による木質系バイオマスからのエタノール発酵

  山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• Creation of cellooligosaccharide‐assimilating E. coli by displaying active BGL on the cell surface using novel anchor protein

  TANAKA Tsutomu

  Enzyme Engineering XXI, Sep. 2011, English, Engineering Conferences International, Colorado. USA, International conference

  Poster presentation


• Corynebacterium glutamicumを用いた高効率なGABA生産技術の開発

  高橋 千尋, 白川 順規, 畑田 一樹, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• BGL 表層提示大腸菌によるセロビオースを基質としたIPA 生産

  相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 花井 泰三

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• バイオナノカプセルを用いた細胞特異的ドラッグデリバリーシステム

  近藤 昭彦, 田中 勉, 荻野 千秋

  第27回日本DDS学会学術集会, Jun. 2011, Japanese, 日本DDS学会, 文京区, Domestic conference

  Oral presentation


• Homo D-lactic acid fermentation from pentose and xylan by metabolically engineered Lactobacillus plantarum

  TANAKA Tsutomu, OKANO Kenji, OGINO Chiaki, KONDO Akihiko

  33rd Symposium on Biotechnology for Fuels and Chemicals, May 2011, English, Society for industrial Microbiology, Seattle, USA, International conference

  Poster presentation


• Cinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividans

  NODA Shuhei, MIYOSHI, Takanori, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  33rd Symposium on Biotechnology for Fuels and Chemicals, May 2011, English, Society for industrial Microbiology, Seattle, USA, International conference

  Poster presentation


• 放線菌を用いたあらゆる炭素源からのケイ皮酸生産

  野田 修平, 三好 孝則, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 放線菌によるトランスグルタミナーゼの大量分泌生産に向けた生産条件の検討

  宮崎 貴也, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 放線菌によるトランスグルタミナーゼの生産

  宮崎 貴也, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 放線菌における表層提示技術に関する研究

  中村 悠佑, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製

  山川 瞬一, YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 多様なバイオマスを燃料とした酵素バイオ燃料電池の開発

  山本 一寛, 松本 拓也, TANAKA Tsutomu, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 酵素を用いたタンパク質高機能化技術の開発

  TANAKA Tsutomu

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発

  森田 健太, 唐崎 美樹, 佐藤 和好, 大原 智, 中山 雅央, 佐々木 良平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発

  森田 健太, 唐崎 美樹, 佐藤 和好, 大原 智, 中山 雅央, 佐々木 良平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• βグルコシダーゼ表層提示によるセロビオース資化性大腸菌の創製

  TANAKA Tsutomu, 川畑 瞳, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• βグルコシダーゼを表層提示したセロビオース資化性大腸菌の創製

  TANAKA Tsutomu, 川畑 瞳, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• ランダム変異導入による高活性エンドグルカナーゼの創製

  中谷 侑紀, YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• バイオマスの直接利用を目指した多機能性電極の開発

  山本 一寛, 松本 拓也, TANAKA Tsutomu, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• トランスポゾンタギング法による好塩性細菌Halomonas elongata OUT30018株の浸透圧ストレス応答性遺伝子の探索

  谷村 幸亮, NAKAYAMA Hideki, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• コリネ型細菌を用いたGABAの生産

  高橋 千尋, 白川 順規, 畑田 一樹, 土舘 健幸, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• Sortaseを用いたStreptavidinの新規修飾技術の開発

  松本 拓也, TANAKA Tsutomu, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• Production of enantiomeric pure lactic acid from biomass by cell surface engineered LAB

  KONDO Akihiko, TANAKA Tsutomu, OKANO, K

  日本農芸化学会2011年度大会, Mar. 2011, English, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• Corynebacterium glutamicumを用いたGABAの高効率生産技術の開発

  高橋 千尋, 白川 順規, 畑田 一樹, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• Biofunctional TiO2 Nanoparticle-mediated Photokilling of Cancer Cells Using UV Irradiation

  TANAKA Tsutomu

  The 16th Symposium of Young Asian Biochemical Engineers' Communyty, Nov. 2010, English, (社)化学工学会, 台湾桃園県, International conference

  Poster presentation


• 放線菌を用いた様々なタンパクの大量分泌系の構築

  野田 修平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 過酸化チタンナノ粒子及び低線量X 線照射を併用した深部ガンの非侵襲的治療法の開発

  唐崎 美樹, 松井 かずさ, 佐藤 和好, 大原 智, 佐々木 良平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• ワイン酵母OC-2 トリプルマーカー株をプラットフォームとした高β – グルコシダーゼ活性と高キシロシダーゼ活性を持つキシロース発酵酵母の作製

  齋藤 聡志, TANAKA Tsutomu, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• セルラーゼ発現バランス最適化酵母を用いたセルロースからのエタノール発酵

  YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• Sortase を用いたStreptavidin の新規修飾技術の開発

  松本 拓也, TANAKA Tsutomu, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• Affibody 提示ナノカプセルを用いたHER2 発現癌細胞特異的な薬物送達

  三枝 宏彰, 宍戸 卓矢, 西村 勇哉, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 放線菌を用いた様々なタンパクの大量分泌系の構築

  野田 修平, TANAKA Tsutomu, KONDO Akihiko, OGINO Chiaki

  2010年度日本放線菌学会大会, Sep. 2010, Japanese, 日本放線菌学会, 東京都 江戸川区, Domestic conference

  Oral presentation


• affibody提示バイオナノカプセルを用いたタンパク質デリバリーシステム

  西村 勇哉, 三枝 宏彰, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference

  Poster presentation


• Construction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells

  KONDO Akihiko, HASUNUMA Tomohisa, OGINO Chiaki, TANAKA Tsutomu

  RENEWABLE ENERGY 2010, Jun. 2010, English, International Solar Energy Society, 横浜市, International conference

  Oral presentation


• 放線菌を用いた様々なタンパクの大量分泌系の構築

  野田 修平, 岡井 直子, 田中 勉, 近藤 昭彦, 荻野 千秋

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• 非侵襲性がん治療に向けた高機能性二酸化チタンナノ粒子の開発

  唐崎 美樹, 松井 かずさ, 瀬川 麻衣子, 田中 勉, 近藤 昭彦, 荻野 千秋

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• 生デンプンからの効率的な繰返し発酵を達成するアミラーゼ発現新規酵母株の創製

  山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• 新規リパーゼ発現系を用いたWhole-cell biocatalystによる効率的なバイオディーゼル燃料生産

  高谷 倫弘, Tamalampudi Sriappareddy, 田中 勉, 中島 一紀, 坊垣 隆之, 和田 純平, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• 酵素を用いた抗体結合ドメインー機能性タンパク質調製法の最適化

  坂本 崇幸, 田中 勉, 近藤 昭彦

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• バイオマスからの燃料・化学品生産を目指した細胞工場の創製

  近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 田中 勉

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• カクテルδインテグレーション法によるセルラーゼ発現バランス最適化酵母の創製

  山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• His-Tagタンパク質特異的精製機能を有する高分子多孔膜の開発

  田林 俊介, 丸山 達生, 田中 勉, 大向 吉景, 松山 秀人

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• Site-specific streptavidin modification using sortase

  松本 拓也, 澤本 詩織, 坂本 崇幸, 田中 勉, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Site-specific protein modification with functional molecule using novel enzyme

  高瀬 了奨, 田中 勉, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Integrated and energy-saving biodiesel fuel production using fungus whole-cell biocatalyst

  甲田 梨紗, 中島 一紀, 田中 勉, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Functional analysis of mutant human somatostatin receptor using a yeast-based fluorescence reporter assay

  TOGAWA Shota, ISHII Jun, TANAKA Tsutomu, KONDO Akihiko

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Expression and signaling analyses of human G protein-coupled receptor in yeast

  IGUCHI Yusuke, ISHII Jun, TANAKA Tsutomu, KONDO Akihiko

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Enzyme-mediated site-specific protein modification

  田中 勉, 坂本 崇幸, 若村 香菜子, 高瀬 了奨, 松本 拓也, 澤本 詩織, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Oral presentation


• Enzyme-mediated protein immobilization on particles

  瀬川 麻衣子, 松井 かずさ, 田中 勉, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Enzyme-mediated antibody-protein conjugation

  坂本 崇幸, 田中 勉, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Enzymatic protein conjugation in vivo

  若村 加菜子, 田中 勉, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Engineering of endoglucanase expression system for Corynebacterium glutamicum

  土舘 健幸, 舘野 俊博, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genes

  山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient homo D-lactic acid production from xylose

  吉田 渉悟, 岡野 憲司, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient ethanol production from xylose by mated diploid Saccharomyces cerevisiae

  角山 裕昭, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient D-lactic acid production from raw starch

  新川 智, 岡野 憲司, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient biodiesel production using whole-cell biocatalyst employing a lipase high expression system

  高谷 倫弘, Sriappareddy Tamalampudi, 中島 一紀, 田中 勉, 坊垣 隆之, 和田 純平, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiae

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Direct fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzyme

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domain

  田渕 聡一郎, 伊藤 純二, 足立 崇, 石田 博樹, 秦 洋二, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling

  FUKUDA Nobuo, ISHII Jun, TANAKA Tsutomu, KONDO Akihiko

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Oral presentation


• Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipases

  足立 大輔, 中島 一紀, 田中 勉, 荻野 千秋, 近藤 昭彦, 福田 秀樹

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Antibody-immobilized TiO2 nanoparticles for cancer therapy

  松井 かずさ, 瀬川 麻衣子, 田中 勉, 近藤 昭彦, 荻野 千秋

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Affibody displaying bionanocapsules for HER2 specific drug delivery

  西村 勇哉, 宍戸 卓矢, 三枝 宏彰, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• 生デンプンから高効率にエタノールを生産する新規酵母の創製

  山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• 癌細胞標的化を目指したAffibody 提示ナノカプセルの開発

  三枝 宏彰, 宍戸 卓矢, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• 遺伝子組換え麹菌を用いたタンパク質大量分泌生産技術の開発

  酒井 翔司, 松田 哲弥, 足立 崇, 久田 博元, 秦 洋二, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• Porin を用いたコリネ菌新規細胞表層提示技術の開発

  畑田 一樹, 舘野 俊博, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling

  Fukuda Nobuo, Ishii Jun, Tanaka Tsutomu, Kondo Akihiko

  The 6th International Forum on Post-Genome Technologies (IFPT'6), Sep. 2009, English, Beijing, China, International conference

  Poster presentation


• 非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発

  松井 かずさ, 荻野 千秋, 田中 勉, 福田 秀樹, 近藤 昭彦

  化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• 新規発現系を用いたWhole-Cell Biocatalystによる効率的なバイオディーゼル燃料生産

  高谷 倫弘, 甲田 梨沙, 足立 大輔, Tamalampudi Sriappareddy, 田中 勉, 坊垣 隆之, 和田 純平, 近藤 昭彦, 福田 秀樹

  化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• ピンポイントDDSを目指したバイオナノカプセルの開発

  西村 勇哉, 宍戸 卓矢, 三枝 宏彰, 田中 勉, 福田 秀樹, 近藤 昭彦

  化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• バイオエタノール生産を目指したスーパー酵母の創製

  近藤 昭彦, 蓮沼 誠久, 田中 勉, 荻野 千秋

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Oral presentation


• タンパク質連結酵素を用いた酵素融合タンパク質調製法の開発

  澤本 詩織, 坂本 祟幸, 田中 勉, 福田 秀樹, 近藤 昭彦

  化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• キシロースからの高効率エタノール生産を目指した宿主酵母株の検討

  角山 裕昭, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• キシロースからの高光学純度D-乳酸生産技術の開発

  吉田 渉悟, 岡野 憲司, 田中 勉, 福田 秀樹, 近藤 昭彦

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• アミラーゼ発現酵母による生デンプンからの効率的な繰り返し発酵プロセスの開発

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• Whole-cell biocatalystを使用したバイオディーゼル燃料生産

  甲田 梨沙, 田中 勉, 近藤 昭彦, 福田 秀樹

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• Construction of novel detection system for protein-protein interaction using yeast

  Kondo Akihiko, Fukuda Nobuo, Ishii Jun, Tanaka Tsutomu

  International Workshop HITS 2009, Feb. 2009, English, Tokyo, Japan, International conference

  Poster presentation


• 未来バイオ燃料生産用スーパー(CBP)微生物の開発

  近藤 昭彦, 荻野 千秋, 田中 勉

  第31回日本分子生物学会年会;第81回日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学学会, 日本生化学会, 神戸市, Domestic conference

  Oral presentation


• 酵母G蛋白質共役型受容体蛍光アッセイシステムによるヒトソマトスタチレセプター細胞外ループドメイン2の変異解析

  石井 純, 松村 静香, 立松 健司, 黒田 俊一, 田中 勉, 藤原 俊伸, 福田 秀樹, 近藤 昭彦

  第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学学会, 日本生化学会, 神戸市, Domestic conference

  Poster presentation


• 温度応答性磁性ナノ粒子を用いた迅速かつ高効率なアフィニティー分子の選択法の確立

  福田 展雄, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  若手フロンティア研究会2008, Dec. 2008, Japanese, 神戸大学研究基盤センター, 神戸市, Domestic conference

  Poster presentation


• Methylester Synthesis By Fungi Immobilized BSPs in Ionic Liquid

  荒井 章吾, 荻野 千秋, 谷野 孝徳, 田中 勉, 福田 秀樹, 近藤 昭彦

  4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Kookmin University, Seoul, International conference

  Poster presentation


• Efficient and direct ethanol production from raw starch by d-integrant polyploid fusants of Saccharomyces cerevisiae

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Seoul, Korea, International conference

  Poster presentation


• 特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用

  近藤 昭彦, 宍戸 卓矢, 田中 勉, 荻野 千秋

  第17回日本バイオイメージング学会, Oct. 2008, Japanese, 日本バイオイメージング学会, 千葉市, Domestic conference

  Oral presentation


• Enzyme-Mediated Site-Specific Protein Conjugation In Vitro and In Vivo

  田中 勉

  2008 “Bioseparation for Biorecognition and Bionanotechnology”, Oct. 2008, English, Hanyang University Bionanotechnology BK Program, Inha University Ultra Procesion Bioseparation Technology ERC, Hanyang University Human Sensing System ERC, Ansan, Korea, International conference

  Oral presentation


• 生デンプンからの高効率D-乳酸生産技術の開発

  新川 智, 岡野 憲司, 張 ショウ, 田中 勉, 福田 秀樹, 近藤 昭彦

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• 酵母蛍光レポーターアッセイによるヒトソマトスタチンレセプター変異体の機能解析

  戸川 翔太, 石井 純, 田中 勉, 松村 静香, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• 酵母でのヒトG蛋白質共役型受容体発現に関する分泌シグナル配列の影響

  井口 裕介, 石井 純, 田中 勉, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• ペプチド転移酵素を用いた細胞内タンパク質連結技術の開発

  若村 香菜子, 田中 勉, 石井 純, 福田 秀樹, 近藤 昭彦

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• セルラーゼ発現酵母によるセルロースからのバイオエタノール生産

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• Whole-cell biocatalystを使用した菜種油からのバイオディーゼル生産

  甲田 梨沙, 田中 勉, 近藤 昭彦, 福田 秀樹

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• 無蒸煮デンプンから効率的にエタノールを生産する高機能酵母の創製

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• 生デンプンを原料とした高光学純度D-乳酸の効率的生産技術の開発

  張 ショウ, 岡野 憲司, 新川 智, 田中 勉, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• 新規酵素を用いたタンパク質への小分子修飾技術

  高瀬 了奨, 田中 勉, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• 酵素処理と電子線照射を組み合わせたセルローストリアセテート繊維の減量加工

  倉田 誠一, 山崎 博貴, 高山 勝己, 石垣 智基, 馬場 俊之, 関 隆之, 田中 勉, 近藤 昭彦, 黒田 浩一, 植田 充美, 末 信一朗

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• 酵素を用いた抗体と機能性タンパク質の新規連結法の開発

  坂本 崇幸, 田中 勉, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• ビオチン提示酵母を用いた新規細胞表層提示システムの開発

  増成 伸介, 田中 勉, 石井 純, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• ビオチン提示バイオナノカプセルを用いるピンポイント薬剤送達システムの開発

  安住 友希, 宍戸 卓矢, 田中 勉, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• バイオディーゼルを目的としたAspergillus oryzae によるリパーゼ共発現系の構築

  足立 大輔, 沼田 崇男, 田中 勉, 近藤 昭彦, 福田 秀樹

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• キチン結合ドメインを用いた麹菌細胞表層提示技術の開発

  田淵 聡一郎, 伊藤 純二, 足立 崇, 石田 博樹, 秦 洋二, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation

• 酵素工学研究会


• 生物工学会


• 化学工学会

• わずかひと振りで目的化合物の生産性を向上させる代謝スパイスの開発

  田中 勉

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025

  本年度は、微生物の菌体表層にG6Pと親和性のあるタンパク質を発現させ、G6Pをキャプチャーさせることで菌体内の代謝が代わりシキミ酸経路が強化される現象に着目し、その評価基盤の構築を進めた。シキミ酸経路の強化は経路の最終産物の1つであるフェニルアラニンを指標としてHPLCにて評価する。グルコースおよびG6Pを培養液に添加しても際立った変化は見られず、外部添加からの表層への濃縮は効果がないことが示された。一方で、トランスポーター破壊株ではG6Pキャプチャーの影響は無くなることから、G6Pの膜間での移動が重要であることが確認できた。グルコース以外の糖類で検討したところ、代謝経路に依存する結果が得られた。これは糖を取り込んだあとの代謝物が影響していることを示唆しており、想定しているメカニズム通りに動いている可能性が高まった。また、フェニルアラニン以外にも経路の異なるアミノ酸であるリジン、および合成経路の異なる有機酸を生産する系をベースにした評価系を構築した。これらにおいても上記と同様の検討を行ったところ、こちらは経路により強化する場合と減少する場合があることが新たに判明した。これより、糖取り込み後の代謝物のいずれかが重要な役割を担っていること、および下流経路に存在するであろう別の代謝中間体が、代謝を変化させる働きをしていることが示唆される。しかし、代謝物のみで代謝を劇的に変化することは難しいと考えられるため、酵素もしくはエフェクタータンパク質などの関与が示唆される。これらの新しい因子の探索は次年度以降引きづつき進める。


• 増殖にとらわれずに様々な前駆体を十分量供給できるプラットフォーム微生物群の構築

  近藤 昭彦, 田中 勉

  日本学術振興会, 科学研究費助成事業, 基盤研究(A), 神戸大学, Apr. 2020 - Mar. 2023

  前年度に引き続き増殖制御の基盤構築と重要な前駆体を蓄積する菌株の構築を進めた。キシロースを炭素源とした増殖制御系を野生株および改変株にそれぞれ導入し、炭素源で増殖を評価した。初めは増殖速度の違いは単純に導入した経路の活性に依存すると思われたが、中間体の蓄積量が一致せず、経路の導入により代謝ネットワークが別途活性化されている可能性が示唆された。この可能性のあるポイントをいくつか破壊していくことで増殖経路をスムーズに流す方向性が見出された。次年度はこの最適化を進める。また、野生株に比べるとその増殖能の低下が見られたが、炭素源の取り込みを改善することで回復できる可能性が示された。これについても遺伝子改変によりこれまで抑制されてきた制御系が発現している可能性があり、その解明についても進めている。前駆体においては、アセチルCoAを基本とする各種CoAについて検討を行った。アセチルCoA、マロニルCoA、などのCoA群を評価するためにモデル化合物の生産系を構築した。グルコース及びキシロースを炭素源として増殖と生産をそれぞれ制御し、どのパラメータが鍵となるかを評価できる系を構築した。解糖系の上流の前駆体においてもモデル化合物の生産系を構築し、増殖と生産を別々に制御できる評価系を構築した。物質生産におけるフィードバック阻害などの多数の制御系を解除するとともに、競合経路の破壊や副生成物の削除などを引き続き次年度も進めていく。


• 糖の使い分けによる高収率物質生産微生物の構築

  田中 勉

  科学研究費補助金／基盤研究(B), Apr. 2019 - Mar. 2022, Principal investigator

  Competitive research funding


• 異種界面での化学結合を伴わないナノ－バイオハイブリッド材料作製技術の開発

  丸山 達生

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2020

  Competitive research funding


• マルチファンクショナルセルラーゼによるバイオマス分解能の拡張とバイオ繊維原料生産

  田中 勉

  科学研究費補助金／若手研究(A), Apr. 2016 - Mar. 2019, Principal investigator

  Competitive research funding


• ネガティブレギュレーションを全て解除した基幹化合物生産細胞工場の創製

  近藤 昭彦

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2019

  Competitive research funding


• 補酵素を必要としないモノマー重合酵素の創製

  近藤 昭彦

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2015 - Mar. 2017

  Competitive research funding


• 【未来社会】 細胞表層工学と代謝工学を用いたＰＥＰ蓄積シャーシ株の創製

  田中 勉

  国立研究開発法人科学技術振興機構, 未来社会創造事業（探索加速型）, 2017, Principal investigator

  Competitive research funding


• わずかな通電で酵素活性と遺伝子発現をそれぞれ制御可能なON/OFFスイッチの開発

  福田 秀樹

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2013 - Mar. 2015

  Competitive research funding


• 工業展開を指向した細胞表層提示の網羅的解析「Displome」の確立

  近藤 昭彦

  科学研究費補助金／基盤研究(B), Apr. 2012 - Mar. 2015

  Competitive research funding


• バイオ燃料･有用ケミカル・電気エネルギーを同時に作り出す生産プロセスの開発

  福田 秀樹

  科学研究費補助金／基盤研究(B), Apr. 2012 - Mar. 2015

  Competitive research funding


• A-STEP「バイオマスからワンステップで乳酸ポリマーを直接生産する技術の開発」

  田中 勉

  研究成果最適展開支援プログラム フィージビリティスタディステージ 探索タイプ, 2013, Principal investigator

  Competitive research funding


• A-STEP「バイオマスからワンステップで乳酸ポリマーを直接生産する技術の開発」

  田中 勉

  研究成果最適展開支援プログラム フィージビリティスタディステージ 探索タイプ, 2012, Principal investigator

  Competitive research funding


• 細胞内外の微小な温度差に応答する磁性ナノ粒子を用いた早期ガン診断・治療法の開発

  近藤 昭彦

  学術研究助成基金助成金／挑戦的萌芽研究, 2011

  Competitive research funding


• 実バイオマスから直接電気エネルギーを生み出す固定化生体触媒の開発

  福田 秀樹

  科学研究費補助金／萌芽研究, 2010

  Competitive research funding


• タンパク質の安定性向上技術の開発とバイオ燃料生産への応用

  田中 勉

  科学研究費補助金／若手研究(B), 2009, Principal investigator

  Competitive research funding


• 非天然型膜構造を持つ有機溶媒耐性型の新規菌体触媒開発

  近藤 昭彦

  科学研究費補助金／基盤研究(B), 2008

  Competitive research funding

• βグルコシダーゼを発現する大腸菌

  KONDO AKIHIKO, OGINO CHIAKI, TANAKA TSUTOMU

  特願2010-258361, 18 Nov. 2010, 大学長, 特許5846523, 04 Dec. 2015

  Patent right


• 油糧粕からのアルコール製造方法

  TANAKA TSUTOMU

  特願2010-054637, 11 Mar. 2010, 大学長, 特許5742102, 15 May 2015

  Patent right


• 放射線治療剤

  OGINO CHIAKI, TANAKA TSUTOMU, SASAKI RYOHEI, KONDO AKIHIKO

  特願2012-500638, 17 Feb. 2011, 大学長, 特許5649137, 21 Nov. 2014

  Patent right


• 高親和性のタンパク質間相互作用検出・スクリーニング方法

  福田 展雄, ISHII JUN, TANAKA TSUTOMU, KONDO AKIHIKO

  特願2010-017509, 28 Jan. 2010, 大学長, 特許5574479, 11 Jul. 2014

  Patent right


• 放射線治療剤(中)

  OGINO CHIAKI, TANAKA TSUTOMU, SASAKI RYOHEI, KONDO AKIHIKO

  201180008541.3, 17 Feb. 2011, 大学長, ZL201180008541.3, 02 Jul. 2014

  Patent right


• 放射線治療剤

  OGINO CHIAKI, TANAKA TSUTOMU, SASAKI RYOHEI, KONDO AKIHIKO

  13/578836, 17 Feb. 2011, 大学長, 8580312, 12 Nov. 2013

  Patent right