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KATSUDA TomohisaGraduate School of Engineering / Department of Chemical Science and EngineeringAssociate Professor
Researcher basic information
■ Research Keyword■ Research Areas
- Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering
- Apr. 2017 - Mar. 2019, The Society of Chemical Engineers, Japan, Member, Kansai Branch
- Mar. 2012 - Feb. 2018, The Society of Chemical Engineers, Japan, Secretary, Division of Biochemical Engineering
- Jun. 2003 - Dec. 2011, The Society for Biotechnology, Member, Kansai Branch
- Apr. 2002 - Oct. 2011, Young Asian Biochemical Engineers’ Community, 日本事務局員
- Oct. 2002 - Dec. 2010, 日本生物工学会関西支部, バイオテクノロジー実験講座講師
- Apr. 2000 - Mar. 2009, 化学工学会関西支部CES21, 運営委員
Research activity information
■ Award- Sep. 2024 The Society of Chemical Engineers, Japan, Outstanding Paper Award of 2023, Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica MatrixOfficial journal
- Nov. 2023 神戸大学工学部, 令和四年度優秀教育賞(応用化学科)
- Nov. 2018 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of influenza virus-like particles in stably transformed insect cellsInternational society
- Oct. 2018 神戸大学工学部, 平成29年度優秀教育賞(応用化学科)
- Sep. 2017 化学工学会, バイオ部会優秀ポスター賞, 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産Japan society
- Nov. 2016 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of virus-like particles using recombinant insect cellsInternational society
- Oct. 2010 Asia Pacific Confederation of Chemical Engineering, Best Poster Paper Award (The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010)), Production of Japanese encephalitis virus-like particles in insect cell expression systemsInternational society
- Feb. 2010 化学工学会関西支部, 化学工学会バイオ部会, 第8回最先端バイオテクノロジー発表会ポスター賞, 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響, 野上達弘
- Dec. 2009 Young Asian Biochemical Engineers’ Community, Best Poster Presentation Award for an Original Contribution to the 15th Symposium of Young Asian Biochemical Engineers’ Community, Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli
- Sep. 2007 神戸大学工学部, 平成18年度優秀教育賞(移動現象論・分離工学分野)
- Sep. 2007 日本生物工学会, 生物工学奨励賞(照井賞), フォトバイオリアクターによる有用物質生産のための生物化学工学的検討
- Mar. 2005 化学工学会, Masaki Naito Memorial Award, 光合成微生物による光培養生産プロセスの開発
- Feb. 2023, Journal of Chemical Engineering of Japan, 56(1) (1), 2179369, English[Refereed]Scientific journal
- Nov. 2022, JOURNAL OF BIOTECHNOLOGY, 359, 15 - 20, English[Refereed][Invited]Scientific journal
- Jun. 2021, CYTOTECHNOLOGY, 73(3) (3), 299 - 305, English[Refereed]Scientific journal
- Jan. 2021, MATEC Web of Conferences, 333, 07009 - 07009, English[Refereed]Scientific journal
- Nov. 2020, BIOCHEMICAL ENGINEERING JOURNAL, 163, 107757 - 107757, English[Refereed]Scientific journal
- Aug. 2020, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 130(2) (2), 205 - 211, English[Refereed]Scientific journal
- May 2020, BMC Proceedings, 14, 14 - 15, EnglishProduction of influenza virus-like particles in insect cellsInternational conference proceedings
- Jan. 2019, Separation and Purification Technology, 209, 193 - 201, English[Refereed]Scientific journal
- Jan. 2019, In Vitro Cellular & Developmental Biology - Animal, 55(1) (1), 1 - 6, English[Refereed]Scientific journal
- Dec. 2018, Bioresource Technology, 269, 489 - 502, English[Refereed]Scientific journal
- Oct. 2018, New Biotechnology, 44S, S159, EnglishInternational conference proceedings
- Apr. 2018, Clean Technologies and Environmental Policy, 20(9) (9), 1 - 12, English[Refereed]Scientific journal
- Mar. 2018, BMC Proceedings, 12(3) (3), 19, EnglishInternational conference proceedings
- Aug. 2017, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124(2) (2), 221 - 226, English[Refereed]Scientific journal
- Jul. 2016, NEW BIOTECHNOLOGY, 33, S200 - S201, EnglishInternational conference proceedings
- Aug. 2015, CYTOTECHNOLOGY, 67(4) (4), 741 - 747, English[Refereed]Scientific journal
- Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 345 - 350, English[Refereed]Scientific journal
- Jan. 2015, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 48(1) (1), 61 - 71, English[Refereed]Scientific journal
- Jul. 2014, NEW BIOTECHNOLOGY, 31, S186 - S186, EnglishInternational conference proceedings
- Feb. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(3) (3), 1071 - 1079, English[Refereed]Scientific journal
- Dec. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(6) (6), 657 - 662, English[Refereed]Scientific journal
- Nov. 2012, International Workshop on Process Intensification 2012, 51 - 52, EnglishDevelopment of Liposome Preparation, Using Gas-Liquid Phase AdsorptionInternational conference proceedings
- Aug. 2012, BIOCHEMICAL ENGINEERING JOURNAL, 67(-) (-), 77 - 82, English[Refereed]Scientific journal
- Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine-Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed.2012, Methods in molecular biology (Clifton, N.J.), 907, 305 - 24, English, International magazineScientific journal
- Jan. 2012, CHEMICAL ENGINEERING & TECHNOLOGY, 35(1) (1), 157 - 160, English[Refereed]Scientific journal
- Apr. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(4) (4), 465 - 470, English[Refereed]Scientific journal
- Feb. 2011, BIOCHEMICAL ENGINEERING JOURNAL, 53(3) (3), 253 - 259, English[Refereed]Scientific journal
- Nov. 2010, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 110(5) (5), 577 - 581, English[Refereed]Scientific journal
- Oct. 2010, The 13th Asia Pacific Confederation of Chemical Engineering Congress Proceedings, 10338Micro-fluidic behavior on liposome production in a micro-channel[Refereed]
- Oct. 2010, The 13th Asia Pacific Confederation of Chemical Engineering Congress Proceedings, 10640, EnglishProduction of Japanese encephalitis virus-like particles in insect cell expression systems[Refereed]International conference proceedings
- Aug. 2010, CYTOTECHNOLOGY, 62(4) (4), 293 - 300, English[Refereed]Scientific journal
- Apr. 2010, PROTEIN EXPRESSION AND PURIFICATION, 70(2) (2), 248 - 253, English[Refereed]Scientific journal
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S93 - S94, English[Refereed]International conference proceedings
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(S1) (S1), S7 - S7, EnglishInternational conference proceedings
- Sep. 2009, Biotechnology Journal, 4(9) (9), 1345 - 1356, English[Refereed]Scientific journal
- Sep. 2009, BIOCHEMICAL ENGINEERING JOURNAL, 46(1) (1), 65 - 68, English[Refereed]Scientific journal
- May 2009, BIOCHEMICAL ENGINEERING JOURNAL, 44(2-3) (2-3), 289 - 291, English[Refereed]Scientific journal
- Apr. 2009, CYTOTECHNOLOGY, 59(3) (3), 153 - 160, English[Refereed]Scientific journal
- Sep. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(3) (3), 310 - 312, English[Refereed]Scientific journal
- In photobioreactors, photosynthetic microorganisms are exposed to certain light/dark cycles caused by light intensity distribution and mixing inside the photobioreactor. In this study, Haematococcus pluvialis was cultivated in an airlift and a bubble column photobioreactor, and the cell growth and astaxanthin production were compared to clarify the effects of liquid circulation.The Society for Biotechnology, Japan, Aug. 2008, Journal of bioscience and bioengineering, 106(2) (2), 204 - 207, English
- Aug. 2008, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 41(8) (8), 739 - 743, English[Refereed]Scientific journal
- Aug. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(2) (2), 204 - 207, English[Refereed]Scientific journal
- Jul. 2008, Microoptics News, 26, 29 - 34, Japanese微生物の光合成を利用した有用物質生産-エネルギーと食品への活用-[Invited]Symposium
- May 2008, BIOCHEMICAL ENGINEERING JOURNAL, 39(3) (3), 575 - 580, English[Refereed]Scientific journal
- 日本生物工学会, Mar. 2008, 生物工学会誌, 86(3) (3), 117 - 122, Japaneseフォトバイオリアクターによる有用物質生産のための生物化学工学的検討[Invited]Research society
- Mar. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105(3) (3), 216 - 220, English[Refereed]Scientific journal
- Nov. 2007, The 8th Asia-Pacific Biochemical Engineering Conference 2007 (Taipei, Taiwan, 2007), C0177Liposome formation characteristics in a micro-tube[Refereed]
- Nov. 2007, Journal of Bioscience and Bioengineering, 104(5) (5), 403 - 407, English[Refereed]Scientific journal
- Aug. 2007, JOURNAL OF CHROMATOGRAPHY A, 1161(1-2) (1-2), 36 - 40, English[Refereed]Scientific journal
- Jan. 2007, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 40(1) (1), 59 - 62, English[Refereed]Scientific journal
- Dec. 2006, Biochemical Engineering Journal, 32(3) (3), 149 - 156, English[Refereed]Scientific journal
- Nov. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(5) (5), 442 - 446, English[Refereed]Scientific journal
- Oct. 2006, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 39(10) (10), 1104 - 1107, English[Refereed]Scientific journal
- Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 220 - 226, English[Refereed]Scientific journal
- Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 206 - 209, English[Refereed]Scientific journal
- Aug. 2006, JOURNAL OF CHROMATOGRAPHY A, 1123(2) (2), 182 - 188, English[Refereed]Scientific journal
- Aug. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(2) (2), 97 - 101, English[Refereed]Scientific journal
- Jun. 2006, FOOD AND BIOPRODUCTS PROCESSING, 84(C2) (C2), 135 - 138, English[Refereed]Scientific journal
- Dec. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100(6) (6), 672 - 677, English[Refereed]Scientific journal
- Sep. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100(3) (3), 339 - 342, English[Refereed]Scientific journal
- May 2005, The 7th Asia-Pacific Biochemical Engineering Conference 2005 (Jeju, Korea, 2005), P2-088Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis[Refereed]
- Apr. 2005, Iranian Journal of Chemical Engineering, 2(2) (2), 15 - 21, EnglishSemi-Continuous Cultivation of Photosynthetic Cells in Flat Plate Photobioreactor[Refereed]Scientific journal
- Dec. 2004, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 37(12) (12), 1445 - 1451, EnglishNeutralization of acids by microorganisms co-immobilized with Bacillus subtilis in a shallow layer of model soil[Refereed]Scientific journal
- Dec. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98(6) (6), 452 - 456, EnglishEffects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis[Refereed]Scientific journal
- Nov. 2004, 2004 AIChE Annual Meeting Conference Proceedings, 483bAstaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps[Refereed]
- Jul. 2004, ENZYME AND MICROBIAL TECHNOLOGY, 35(1) (1), 81 - 86, English[Refereed]Scientific journal
- May 2004, BIOTECHNOLOGY PROGRESS, 20(3) (3), 998 - 1000, English[Refereed]Scientific journal
- From engineering point of view, the effect of light intensity distribution on the stability of growth rate can play an important role in designing of effective photobioreactors and sustaining of stable growth rates. In these series of experiments, in order to keep the operational parameters at almost constant level, a semi-continuous culture method was developed. In this cultivation method, a part of culture broth containing grown cells was repeatedly replaced with the fresh medium at a predetermined time interval to keep the cell concentration and the volume of the broth constant at initial values. Thus it was possible to cultivate photosynthetic cells under defined light intensity distributions.The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 3P-01-085, 807 - 807, English
Under one side and both side illumination and also reflection by mirror photosynthetic cells were cultivated in flat plate photobioreactors with various light path lengths. The obtained results showed that stability of the growth rate strongly depended on the distribution of light intensity and ratio of light intensity at light zone to that at dark zone inside photobioreactors. These parameters should be taken into consideration for stable cultivation of photosynthetic cells.[Refereed]International conference proceedings - Haematococcus pluvialisis is one of the potential candidates for production of astaxanthin. Growth characteristics of H. pluvialis in batch run and medium replacement runs were measured. A two-stage process was employed for biomass and astaxanthin synthesis by this alga. During the first stage, medium was replaced several times every day to achieve high cell concentration. When the replacement was stopped, the light illumination was switched from red LEDs at 8μmol photons m-2 s-1 to blue LEDs at 12µmol photons m-2 s-1 to stimulate the accumulation of astaxanthin without decreasing in the cell numbers by death of the cells. H. pluvialis exhibited relatively high cell concentration in the first step and reached to about 1.5 mg-dry cell/cm3 and accumulated up to 65 µg/cm3 astaxanthin in the second stage, which are higher than those reported in literature.The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 3L-07, 430 - 430, English[Refereed]International conference proceedings
- In the present work, we investigated the effects of GC content, mismatching position, and length on the thermostability of double-strand oligo-DNAs in order to develop an affinity chromatography using oligonucleotide-immobilized capillary for SNP detection. We found that a single mismatching base pair appeared on 25 mer fragment of human tp53 tumor suppressor protein gene could reduce the melting temperature by 6.6°C. It seemed to be enough for eluting the mutated fragment separately from the intact one during a course of temperature ascending process.The Society of Chemical Engineers, Japan, 2004, The 10th Asian Pacific Confederation of Chemical Engineering Congress Proceedings, 1P-01-009, 479 - 479, English[Refereed]International conference proceedings
- Jan. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(1) (1), 54 - 58, EnglishCharacteristics of neutralization of acids by newly isolated fungal cells[Refereed]Scientific journal
- Jun. 2003, Chemical Engineering Transactions, 3, 1671 - 1675Cultivation of Microalga in Photobioreactors Illuminated with LEDs[Refereed]
- Oct. 2002, Journal of Chemical Engineering of Japan, 35(10) (10), 963 - 968, English[Refereed]Scientific journal
- May 2002, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 35(5) (5), 428 - 435, EnglishLight attenuation in suspension of the purple bacterium Rhodobacter capsulatus and the green alga Chlamydomonas reinhardtii[Refereed]Scientific journal
- Jun. 2001, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 34(6) (6), 743 - 747, EnglishControl of aggregate formation in refolding of carbonic anhydrase at high urea concentrations and effects of urea removal[Refereed]Scientific journal
- Apr. 2001, 神戸大学ベンチャー・ビジネス・ラボラトリー年報, 6, 185 - 190, Japanese光合成微生物の懸濁液中における光強度減衰Research institution
- Jun. 2000, Biochemical Engineering Journal, 5(2) (2), 157 - 164, English[Refereed]Scientific journal
- Jan. 2000, Bioscience, Biotechnology and Biochemistry, 64(2) (2), 248 - 253, English[Refereed]Scientific journal
- Nov. 1999, The 5th Asia-Pacific Biochemical Engineering Conference 1999 (Phuket, Thailand, 1999), P-BP9Light distribution model for the externally illuminated cylindrical photo-bioreactor and its application for production of hydrogen by Rhodobacter capsulatus[Refereed]
- The characteristics of Rhodobacter capsulatus ST410, a mutant of the wild strain B100 lacking hydrogenase activity, were investigated from the viewpoint of hydrogen production. When 30 mM DL-malate and 7 mM L-glutamate were used as carbon and nitrogen sources, respectively, in an argon atmosphere, a specific hydrogen evolution rate of 0.14 ml/h/mg-dry cells was obtained at 6600 lx and 33℃. The evolution rate strongly depended on the light intensity : the higher the light intensity, the larger the evolution rate became up to at least 6600 lx. R. capsulatus ST410 converted 60 mM malate to hydrogen at a yield of 68%, calculated as a percentage of the stoichiometric maximum for the complete conversion of the carbon source to H_2 and CO_2. On the other hand, when the wild strain was used under the same conditions, the yield was only 25%. R. capsulatus ST410 converted not only malate but also glucose and cellobiose to hydrogen with good yields(60% for 30mM glucose and 66% for 7.5 mM cellobiose). Ethanolamine was found to be a good nitrogen source, which permitted a large amount of hydrogen to be evolved and also depressed the cell growth to low levels.Elsevier BV, Jan. 1998, Journal of Fermentation and Bioengineering, 85(5) (5), 470 - 475, English[Refereed]Scientific journal
- Feb. 1997, Kagaku Kogaku Symposium Series 57 Horizon of Biochemical Engineering, 57, 81 - 85, EnglishHydrogen Detectable Color Indicator for Screening of MicroorganismsSymposium
- Feb. 2008, 生物工学会誌, 86(2) (2), 84 - 84, JapaneseCCSと微細藻類による有用物質生産Introduction scientific journal
- 日本生物工学会, Mar. 2002, 生物工学会誌, 80(3) (3), 128 - 128, Japanese光合成微生物による水素生産のゆくえIntroduction scientific journal
- Others, Springer Science+Business Media, Aug. 2012, EnglishProduction of antibody fragments in Escherichia coli. In: Patrick Chames (ed.), Antibody Engineering: Methods and Protocols, Second Edition, Method in Molecular Biology, vol. 907, pp. 305-324 (Chapter 18)Scholarly book
- Others, CMC Publishing, Jun. 2010, Japanese抗体医薬のための細胞構築と培養技術Scholarly book
- Joint work, シーエムシー出版, Sep. 2005, JapaneseFrontier of Eco-Bio EnergyScholarly book
- SCEJ 55th Autumn Meeting, Sep. 2024, English, SapporoUsing Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix[Invited]Invited oral presentation
- SCEJ 89th Annual Meeting, Mar. 2024, JapaneseEfficient transfection of insect cells with controlling cell cycle progressionPoster presentation
- 化学工学会第54回秋季大会, Sep. 2023, EnglishProduction of influenza virus-like particles displaying heterologous proteinsPoster presentation
- 化学工学会第88年会, Mar. 2023昆虫細胞を用いたキメラインフルエンザウイルス様粒子の生産Poster presentation
- 化学工学会第88年会, Mar. 2023培養温度と光強度を概日周期で制御したヘマトコッカス プルビアリスによるアスタキサンチン生産Poster presentation
- 化学工学会第87年会, Mar. 2022, EnglishAn investigation of diffusion and adsorption behavior in protein A mediaPoster presentation
- 化学工学会第87年会, Mar. 2022, English異種タンパク質を提示するインフルエンザウイルス様粒子の昆虫細胞を用いた生産Poster presentation
- 日本生物工学会100周年記念事業教育セミナー「培養技術勉強会」, Nov. 2021, Japanese分離精製(膜・クロマト分離)[Invited]Public discourse
- 化学工学会第52回秋季大会, Sep. 2021遺伝子組換え昆虫細胞樹立へのゲノム編集技術の応用
- 第34回日本動物細胞工学会2021年度大会 (JAACT2021), Jul. 2021ゲノム編集技術を用いた遺伝子組換え昆虫細胞の樹立
- 化学工学会第86年会, Mar. 2021, JapaneseプロテインA固定化マイクロアフィニティカラムを用いた疑似オンラインIgG濃度測定法の開発Poster presentation
- The 33rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2020 Fuchu), Nov. 2020Production of influenza virus-like particles using recombinant insect cells
- 化学工学会第51回秋季大会, Sep. 2020, Japanese昆虫細胞を用いたインフルエンザウイルス様粒子の生産
- Young Asian Biological Engineers' Community Symposium 2019, Nov. 2019, English, Young Asian Biological Engineers' Community, Seoul, Korea, Republic ofEffects of the circadian control in temperature and light intensity on the cell division of green algae under a photoautotrophic conditionPoster presentation
- 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, EnglishAdsorption of immunoglobulin G in protein A resins studied by confocal laser scanning microscopy taking account of the optical hindrance inside a particlePoster presentation
- 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, EnglishProduction of influenza virus proteins in recombinant insect cellsPoster presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese微細藻の細胞分裂に及ぼす培養温度・光強度の周期的制御の影響Oral presentation
- 第32回日本動物細胞工学会2019年度大会 (JAACT2019), Jul. 2019, Japanese組換え昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産Poster presentation
- 26th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2019), May 2019, EnglishProduction of influenza virus-like particles in insect cellsPoster presentation
- 化学工学会第84年会, Mar. 2019, Japanese, 化学工学会, 東京, Domestic conference固定化したシトクロムP450発現大腸菌によるwhole cell bioconversionPoster presentation
- 31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, 日本動物細胞工学会, 筑波, International conferenceProduction of influenza virus-like particles in stably transformed insect cellsPoster presentation
- 31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, 日本動物細胞工学会, 筑波, International conferenceProduction of antibody Fab fragment using 2A peptide in insect cellsPoster presentation
- 化学工学会第50回秋季大会, Sep. 2018, Japanese, 化学工学会, 鹿児島, Domestic conference緑藻Chlorella sorokinianaの光独立栄養培養における培養温度の影響Poster presentation
- 化学工学会第50回秋季大会, Sep. 2018, Japanese, 化学工学会, 鹿児島, Domestic conference培養温度・光強度の周期的な変化が緑藻Haematococcus pluvialisの細胞分裂に及ぼす影響Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会大会, 吹田, Domestic conference昆虫細胞における2Aペプチドを用いた抗体生産Oral presentation
- 化学工学会第50回秋季大会, Sep. 2018, English, 化学工学会, 鹿児島, Domestic conferenceゲノム編集技術を用いた組換え昆虫細胞の作製Poster presentation
- 18th European Congress on Biotechnology (ECB2018), Jul. 2018, English, European Federation of Biotechnology, Geneva, Switzerland, International conferenceProduction of influenza virus proteins in stably transformed insect cellsPoster presentation
- 化学工学会第83年会, Mar. 2018, Japanese, 化学工学会, 吹田, Domestic conference緑藻Haematococcus pluvialisにおける周期的温度変化と細胞増殖の関係Poster presentation
- 化学工学会第83年会, Mar. 2018, Japanese, 化学工学会, 吹田, Domestic conference昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産Poster presentation
- 化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産Poster presentation
- 化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference共焦点レーザー走査顕微鏡観察と数値ミュレーションに基づく抗体の吸着・破過の解析Poster presentation
- 化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conferenceエアリフト式フォトバイオリアクターの操作条件と微細藻類の光独立栄養増殖の関係Poster presentation
- International Meeting & 42nd Annual Conference of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Aug. 2017, English, The Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Kuala Lumpur, Malaysia, International conferenceProduction of influenza virus proteins by recombinant insect cellsPoster presentation
- 第30回日本動物細胞工学会2017年度大会 (JAACT2017), Jul. 2017, Japanese, 日本動物細胞工学会, 松山, Domestic conference昆虫細胞を用いたインフルエンザウイルスタンパク質の生産Poster presentation
- 25th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2017), May 2017, English, European Society for Animal Cell Technology (ESACT), Lausanne, Switzerland, International conferenceEfficient protein production by transient gene expression using insect cellsPoster presentation
- The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conferenceTransient gene expression in insect cells for efficient recombinant antibody productionPoster presentation
- The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conferenceProduction of virus-like particles using recombinant insect cellsPoster presentation
- The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conferenceCharacterization of the diffusion and adsorption of IgG in protein A-coupled adsorbents with confocal laser scanning microscopyPoster presentation
- 17th European Congress on Biotechnology (ECB2016), Jul. 2016, English, European Federation of Biotechnology (EFB), Krakow, Poland, International conferenceEfficient antibody production by transient gene expression in insect cellsPoster presentation
- 化学工学会第81年会, Mar. 2016, Japanese, 化学工学会, 大阪, Domestic conference培養条件の概日変化が緑藻Haematococcus pluvialisの栄養増殖に及ぼす影響Poster presentation
- 化学工学会第81年会, Mar. 2016, Japanese, 化学工学会, 大阪, Domestic conference共焦点レーザー走査顕微鏡観察に基づくProtein A固定化吸着剤の拡散・吸着特性の評価Oral presentation
- Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conferenceRecombinant antibody production by transient gene expression in insect cellsPoster presentation
- Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conferencePhotoautotrophic growth of Haematococcus pluvialis under controlled temperature and light conditionsPoster presentation
- Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conferenceOptimization of microalgal cultivation in cascade photobioreactors with wavy bottom via computational fluid dynamicsPoster presentation
- 化学工学会第47回秋季大会, Sep. 2015, Japanese, 化学工学会, 札幌, Domestic conferenceシトクロムP450発現大腸菌を用いたwhole cell bioconversionに及ぼす培養・反応条件の影響Poster presentation
- 第28回日本動物細胞工学会2015年度大会 (JAACT2015), Jul. 2015, Japanese, 日本動物細胞工学会, 仙台, Domestic conference昆虫細胞を宿主とした一過性発現による抗体タンパク質の生産Poster presentation
- 24th ESACT Meeting Barcelona 2015, Jun. 2015, English, European Society for Animal Cell Technology (ESACT), Barcelona, Spain, International conferenceProduction of an antibody molecule by transient gene expression in insect cellsPoster presentation
- 日本LCA学会, Mar. 2015, Japanese, Domestic conference鉛に汚染された土壌の評価とバイオレメディエーションによる鉛の回収Oral presentation
- 化学工学会姫路大会, Dec. 2014, Japanese, 化学工学会関西支部, 姫路, Domestic conferenceカスケード型フォトバイオリアクターにおける微細藻類培養の数値流体力学に基づく検討Poster presentation
- The 27th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT2014 Kitakyushu), Nov. 2014, English, 日本動物細胞工学会, 北九州, International conferenceTransient gene expression in insect cells for recombinant antibody productionPoster presentation
- 化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡, Domestic conference昆虫細胞を宿主とした一過性発現による抗体タンパク質生産Poster presentation
- 16th European Congress on Biotechnology (ECB16), Jul. 2014, English, European Federation of Biotechnology, Edinburgh, UK, International conferenceProduction of antibody fragments by transient gene expression in insect cellsPoster presentation
- 化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference昆虫細胞を用いた一過性発現による機能性抗体タンパク質の生産Poster presentation
- 化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conferenceHaematococcus pluvialisの増殖とアスタキサンチン生産に及ぼす培養温度の影響Poster presentation
- 化学工学会第78年会, Mar. 2013, English, 化学工学会, 豊中市, Domestic conferencePhotobioreactor design using CFD: mixing and light effects on microalgal culturesOral presentation
- The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, English, 日本動物細胞工学会, 名古屋市, International conferenceProduction of recombinant antibody molecules in insect cellsPoster presentation
- The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, English, 日本動物細胞工学会, 名古屋市, International conferenceProduction and purification of Japanese encephalitis virus-like particles from recombinant insect cellsPoster presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference組換え昆虫細胞によるscFv-Fc融合タンパク質の生産Oral presentation
- 第64回日本生物工学会大会, Oct. 2012, English, 日本生物工学会, 神戸市, Domestic conferenceComputational fluid dynamics for enhancing the light distribution in a photobioreactor grown culture of Hematococcus pluvialisOral presentation
- 化学工学会第44回秋季大会, Sep. 2012, Japanese, 化学工学会, 仙台市, Domestic conference大腸菌を用いた一本鎖抗体生産における終止コドンの影響Poster presentation
- 化学工学会第44回秋季大会仙台, Sep. 2012, Japanese, 仙台, Domestic conference気液界面を利用した高収率リポソーム生成法の開発Oral presentation
- 化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 東京, Domestic conference昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産Oral presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference炭化水素資化酵母Yarrowia lipolyticaによる油汚染土壌の修復のための無機栄養源の開発Poster presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference昆虫細胞を用いた抗体タンパク質の分泌生産Poster presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討Poster presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conferenceシャイン・ダルガーノ配列を改変した大腸菌による一本鎖抗体の流加培養生産Poster presentation
- IUMS 2011 Sapporo Congress, Sep. 2011, English, International Union of Microbiological Societies, 札幌, Domestic conferenceEfficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cellsPoster presentation
- ESACT 2011 Meeting, May 2011, English, European Society for Animal Cell Technology, Vienna, Austria, International conferenceSecretory production of virus-like particles by recombinant insect cellsPoster presentation
- 第13回化学工学会学生発表会(神戸大会), Mar. 2011, Japanese, 化学工学会, 神戸市, Domestic conference炭化水素資化酵母Yarrowia lipolyticaを用いた油汚染土壌の修復における栄養源の添加条件の検討Oral presentation
- 第13回化学工学会学生発表会(神戸大会), Mar. 2011, Japanese, 化学工学会, 神戸市, Domestic conference大腸菌を用いた一本鎖抗体生産におけるシャイン・ダルガーノ配列の影響Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 化学工学会, 東京都, Domestic conference大腸菌におけるインクルージョンボディ生成に及ぼすシャイン・ダルガーノ配列の影響Oral presentation
- 近畿バイオインダストリー振興会議第5回フォローアップ勉強会, Jan. 2011, Japanese, 近畿バイオインダストリー振興会議, 大阪市, Domestic conferenceリポソームの応用事例Invited oral presentation
- The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), Dec. 2010, English, Canadian Society for Chemistry, American Chemical Society, Chemical Society of Japan, New Zealand Institute of Chemistry, Royal Australian Chemical Institute, Korean Chemical Society, Chinese Chemical Society, Honolulu, USA, International conferenceOptimized Shine-Dalgarno sequence for efficient production of single-chain Fv antibody in Escherichia coliPoster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, English, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, International conferenceRapid production of functional antibody molecules by transient gene expression in insect cellsPoster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, English, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, International conferenceEffect of signal peptide on the production of single-chain fv antibody by Escherichia coliPoster presentation
- The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010), Oct. 2010, English, Asia Pacific Confederation of Chemical Engineering, Taipei, Taiwan, International conferenceProduction of Japanese encephalitis virus-like particles in insect cell expression systemsPoster presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市マイクロ流路内におけるリポソーム生成時のマイクロ流体挙動に関する研究Oral presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conferenceプロテインAアフィニティクロマトグラフィーのための溶離条件検討法の開発Poster presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conferenceシャペロン共発現による大腸菌での一本鎖抗体 (scFv) 融合タンパク質の生産Poster presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conferenceアルカン資化酵母Yarrowia lipolyticaを用いた土壌汚染油の分解Poster presentation
- The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010), Sep. 2010, English, The Japanese Association for Animal Cell Technology, 札幌市, International conferenceProduction of Japanese encephalitis virus-like particles using insect cellsPoster presentation
- 化学工学会第75年会, Mar. 2010, Japanese, 化学工学会, 鹿児島, Domestic conferencePichia pastorisを用いた一本鎖抗体生産における誘導初期のメタノール濃度の影響Oral presentation
- 第8回最先端バイオテクノロジー発表会, Feb. 2010, Japanese, 化学工学会, 大阪, Domestic conference昆虫細胞-バキュロウイルス系を用いた抗体タンパク質の高効率生産Poster presentation
- 第8回最先端バイオテクノロジー発表会, Feb. 2010, Japanese, 化学工学会, 大阪, Domestic conference一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響Poster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2009, Dec. 2009, English, Young Asian Biochemical Engineers Community, Xiamen, International conferenceEffects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coliPoster presentation
- Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC '09), Nov. 2009, English, APBioChEC '09 Committee, 神戸市, International conferenceProduction of Japanese encephalitis virus-like particles by recombinant insect cellsOral presentation
- The 9th Asia-Pacific Biochemical Engineering Conference 2009, Nov. 2009, English, The 9th Asia-Pacific Biochemical Engineering Conference 2009, Kobe, International conferenceOil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolyticaPoster presentation
- 化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference大腸菌を用いた一本鎖抗体生産におけるSD配列の影響Poster presentation
- 第61回日本生物工学会大会, Sep. 2009, Japanese, 日本生物工学会, 名古屋市, Domestic conference昆虫細胞を用いた日本脳炎ウイルス様粒子の生産Oral presentation
- 化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference昆虫細胞-バキュロウイルス系による抗体タンパク質の生産Poster presentation
- 化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conferenceヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用Poster presentation
- 化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference昆虫細胞を用いた日本脳炎ウイルスタンパク質の生産Oral presentation
- 化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference一塩基変異を検出する昇温溶出アフィニティクロマトグラフィーに対するPCRの適用性Oral presentation
- 化学工学会第74年会, Mar. 2009, 化学工学会, 横浜, Domestic conferenceマイクロ流路内におけるリポソーム生成機構Oral presentation
- The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, English, 日本動物細胞工学会, 福岡市, International conferenceProduction of adenovirus vector by 293 cells immobilized within porous biomass support particlesPoster presentation
- The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, English, 日本動物細胞工学会, 福岡市, International conferenceLow multiplicity infection of 293 cells for adenovirus vector productionPoster presentation
- The 14th Symposium of Young Asian Biochemical Engineers’ Community, Nov. 2008, English, Young Asian Biochemical Engineers’ Community, 東京都, International conferenceHigh efficiency expression of soluble scFv in E. coli by use of linkers containing rare codonsPoster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2008, Nov. 2008, English, Young Asian Biochemical Engineers Community, Tokyo, International conferenceEffects of the parameters of flashing light from blue emitting diodes on astaxanthin production by H. pluvialisPoster presentation
- The 2 nd SCEJ (Kansai-Branch)/SSCCI Joint International Conference on Chemical Engineering, Nov. 2008, English, SCEJ (Kansai-Branch) and SSCCI, Shanghai, China, International conferenceA biochemical engineering study of photo-bioproces[Invited]Invited oral presentation
- “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, English, Hanyang University, Ansan, Korea, Domestic conferenceSecretory production of Japanese encephalitis virus E protein by recombinant insect cellsPoster presentation
- “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, English, Hanyang University, Ansan, Korea, Domestic conferenceDetection of single nucleotide variation in amplified gene fragments using oligonucleotide-coupled affinity columnPoster presentation
- 化学工学会第40回秋季大会, Sep. 2008, Japanese, 化学工学会, 仙台市, Domestic conference多孔性粒子に固定化した大腸菌を用いる有用物質生産Poster presentation
- 化学工学会第40回秋季大会, Sep. 2008, Japanese, 化学工学会, 仙台市, Domestic conferenceレアコドンリンカーによる大腸菌での可溶性scFvの高効率生産Poster presentation
- 第108回微小光学研究会, Jul. 2008, Japanese微生物の光合成を利用した有用物質生産ーエネルギーと食品への活用ー[Invited]Public discourse
- 11th Liposome Research Days Conference, Jul. 2008, English, Yokohama, Japan, International conferenceLiposome Formation Characteristics with a Micro-ReactorOral presentation
- American Society for Microbiology 108th General Meeting, Jun. 2008, English, American Society for Microbiology, Boston, USA, International conferenceOxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen speciesPoster presentation
- 化学工学会 第73年会, Mar. 2008, Japanese, 静岡, Domestic conferenceマイクロ流路を用いたリポソームの生成機構Oral presentation
- 化学工学会第73年会, Mar. 2008, English, 化学工学会, 浜松, Domestic conferenceEnhancement of growth and astaxanthin production of Haematococcus pluvialis by using airlift photobioreactorOral presentation
- 第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conferenceストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化Oral presentation
- 第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conferenceシリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価Oral presentation
- 第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, English, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conferenceHigh efficiency production of astaxantin by autotrophic cultivation of Haematococcua pluvialis in column photobioreactorsOral presentation
- 上海市化学化工学会・化学工学会関西支部 第1回若手研究交流会, Dec. 2007, English, 上海市化学化工学会、化学工学会関西支部, 大阪, International conferenceEffects of the intensity and frequency on H. pluvialis grown under flashing light illuminationPoster presentation
- 化学工学会山口大会, Nov. 2007, Japanese, 化学工学会中国四国支部, 宇部, Domestic conference青色点滅光照射下でのH. pluvialisの培養に及ぼす光強度および周波数の影響Poster presentation
- The 6th Asia-Europe Biorecognition Engineering Society Conference, Nov. 2007, English, Asia-Europe Biorecognition Engineering Society, Taoyuan, Taiwan, International conferenceApplication of PCR to Affinity Chromatography for SNP Detection[Invited]Invited oral presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conferenceOxidative treatment of H. pluvialis with the sonocatalytic reactionPoster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conferenceEffects of the intensity and frequency on H. pluvialis grown under flashing light illuminationPoster presentation
- 2007 Fall KSBB Meeting & International Symposium, Oct. 2007, English, The Korean Society for Biotechnology and Bioengineering, Daegu, Korea, International conferenceA Biochemical Engineering Study of Bioproductions using Photobioreactor[Invited]Invited oral presentation
- 第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導Oral presentation
- 化学工学会第39回秋季大会, Sep. 2007, Japanese, 化学工学会, 札幌, Domestic conference気泡塔型フォトバイオリアクターを用いたヘマトコッカス藻による効率的アスタキサンチン生産Poster presentation
- 化学工学会 第39回秋季大会, Sep. 2007, Japanese, 北海道大学, Domestic conferenceマイクロキャピラリー内におけるリポソーム生成特性に関する研究Oral presentation
- 第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conferenceフォトバイオリアクターによる 有用物質生産のための生物化学工学的検討[Invited]Others
- 13th European Congress on Biotechnology, Sep. 2007, English, European Federation of Biotechnology, Barcelona, Spain, International conferenceInduction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasoundsPoster presentation
- 化学工学会第39回秋季大会, Sep. 2007, Japanese, 化学工学会, 札幌, Domestic conferenceDNAアフィニティカラムを用いるSNP検出法へのPCR増幅法の応用Poster presentation
- 化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference青色点滅光照射下のH. pluvialisにおけるアスタキサンチン生産量と光強度との関係Oral presentation
- 化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conferenceメタノール資化性酵母を用いた一本鎖抗体生産におけるメタノール供給方法Oral presentation
- 化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conferenceシリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価Oral presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, English, Young Asian Biochemical Engineers Community, Kaohsiung, International conferenceOptimization of silica based media for antibody purification by protein A affinity chromatographyPoster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, English, Young Asian Biochemical Engineers Community, Kaohsiung, International conferenceEffects of flashing light from blue LEDs on the cell growth and astaxanthin production of Haematococcus pluvialisPoster presentation
- 26th international symposium on the separation of proteins, peptides and polynucleotides, Oct. 2006, English, ISPPP, Innsbruck, International conferenceOptimization of silica-based matrices for antibody purification by protein A affinity chromatographyOral presentation
- 化学工学会バイオ部会員フォーマルセミナー, Sep. 2006, Japanese, 化学工学会バイオ部会, 福岡, Domestic conference昇温溶出アフィニティークロマトグラフィーによる一塩基多型検出法の開発Oral presentation
- 第58回生物工学会大会, Sep. 2006, Japanese, 生物工学会, 大阪, Domestic conferenceメタノール資化性酵母Pichia pastorisの連続培養による一本鎖抗体生産Oral presentation
- 第58回生物工学会大会, Sep. 2006, Japanese, 生物工学会, 大阪, Domestic conferenceヘマトコッカスによるアスタキサンチン生産における青色点滅光照射の影響Oral presentation
- 化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conferenceヘマトコッカスによるアスタキサンチン生産における青色パルス光照射条件の検討Oral presentation
- 化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conferencePseudomonas sp. を用いる自己固定化バイオレメディエーションによるシアヌル酸分解Oral presentation
- 化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conferencePhotocultivation of Haematococcus pluvialis Using Multi-Compartment PhotobioreactorOral presentation
- 化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conferenceSNP Detection Method Using Temperature Gradient Affinity ChromatographyOral presentation
- 化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conferenceFlashing light Illumination for Effective Production of Astaxanthin by Haematococcus pluvialisOral presentation
- 関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conferenceEffects of Iron Ion Concentration on the Growth Stability of Photosynthetic Bacteria in the Semi-Continuous CultivationOral presentation
- 関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conferenceBioremediation of Acidified Pond and SoilOral presentation
- 関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conferenceEffects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatusPoster presentation
- 関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conferenceDetection of Single Nucleotide Polymorphysms by Affinity Chromatography with DNA Coupled ColumnPoster presentation
- 平成17年度日本生物工学会大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conferenceBioremediation of Herbicide-Polluted Soil with Self-Immobilized MicroorganismsOral presentation
- 25th International Symposium on the Separation of Proteins, Peptides & Polynucleotides, Nov. 2005, English, International Symposium on the Separation of Proteins, Peptides & Polynucleotides, St. Pete Beach, Florida, International conferenceRapid SNP Detection by Temperature Gradient Affinity ChromatographyOral presentation
- 関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conferenceEffective Production of Astaxanthin from Haematococcus pluvialis by Use of Light Emitting DiodesPoster presentation
- Young Asian Biochemical Engineers Community (YABEC) Symposium 2005, Oct. 2005, English, Young Asia Biochemical Engineers Community, Beijing, China, International conferenceDetection of SNP Using Affinity Chromatography of Immobilized OligonucleotideOral presentation
- 化学工学会第37回秋季大会, Sep. 2005, Japanese, 化学工学会, 岡山, Domestic conferenceEffects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatusPoster presentation
- The 12th European Congress on Biotechnology, Aug. 2005, English, European Federation of Biotechnology, Copenhagen, Denmark, International conferenceGrowth Sstability of Photosynthetic Bacteria : Effects of Supplemental Illumination and Ferrous Ion ConcentrationPoster presentation
- The 12th European Congress on Biotechnology, Aug. 2005, English, European Federation of Biotechnology, Copenhagen, Denmark, International conferenceFed-Batch Cultivation of Haematococcus pluvialis under Illumination with LEDs for Production of AstaxanthinPoster presentation
- 分離技術会年会2005, Jun. 2005, English, 分離技術会, 大阪, Domestic conferenceTemperature Gradient Elution of DNAs from an Oligo-DNA Immobilized ColumnPoster presentation
- Asia-Pacific Biochemical Engineering Conference 2005, May 2005, English, The Korean Society for Biotechnology and Bioengineering, The Korean Institute of Chemical Engineers, Jeju, Korea, International conferenceEffects of Feeding Method on Astaxanthin Production by Haematococcus pluvialisPoster presentation
- The 4th Asia-Europe Biorecognition Engineering Society Conference, May 2005, English, Asia-Europe Biorecognition Engineering Society, Ansan, Korea, International conferenceDetection of SNP using Affinity Chromatography of Immobilized OligonucleotideOral presentation
- 化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conferenceEffects of Nutrients Feeding Method on Astaxanthin Production by Haematococcus pluvialisOral presentation
- 化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conferenceElution Behavior of DNA from Oligo-DNA Immobilized Column under Temperature GradientOral presentation
- 2004 AIChE Annual Meeting, Nov. 2004, English, American Institute of Chemical Engineers, Austin, TX, International conferenceAstaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED LampsOral presentation
- The 2nd International Conference on Bioseparation Engineering, Oct. 2004, English, International conferenceDevelopment of Oligonucleotide Immobilized Capillary ChromatographyOral presentation
- 日本生物工学会平成16年度大会, Sep. 2004, Japanese, 日本生物工学会, 名城大学, Domestic conferenceフォトバイオリアクターにおける光強度分布と増殖速度安定性の工学的検討Oral presentation
- 日本生物工学会平成16年度大会, Sep. 2004, Japanese, 日本生物工学会, 名城大学, Domestic conferenceオリゴDNA固定化担体を用いた一塩基多型(SNP)の検出法の検討Oral presentation
- Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, English, Young Asian Biochemical Engineers' Community (YABEC), Osaka, Japan, International conferenceInstability of Growth Rate in Dense Culture of Photosynthetic CellsOral presentation
- Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, English, Young Asian Biochemical Engineers' Community (YABEC), Osaka, Japan, International conferenceInduction of Astaxanthin Accumulation in H. pluvialis by IIIumination with LED Lamps and Fed Batch Addition of NutrientsOral presentation
- 化学工学会第69年会, Apr. 2004, English, 化学工学会, 大阪府立大学, Domestic conferenceGrowth and Astaxanthin Accumulation in H. pluvialis Cultures Illuminated by LED LampsOral presentation
- 第55回日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本大学, Domestic conference緑藻Haematococcus pluvialisによるアスタキサンチン生産におよぼす青色パルス光の影響Oral presentation
- 第55回日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本大学, Domestic conference光合成微生物の半連続培養における増殖安定性におよぼす光強度分布の影響Oral presentation
- 化学工学会 第36回秋季大会, Sep. 2003, Japanese, 化学工学会, 東北大学, Domestic conferenceパルス光照射下におけるヘマトコッカスによるアスタキサンチン生産Oral presentation
- 化学工学会 第36回秋季大会, Sep. 2003, English, 化学工学会, 東北大学, Domestic conferenceThe effects of light intensity distribution on the stability of growth rate of a photosynthetic microorganism Rhodobacter capsulatusOral presentation
- The 6th Italian Conference on Chemical and Process Engineering, Jun. 2003, English, 未記入, 未記入, International conferenceCultivation of microalga in photobioreactors illuminated with LEDsOral presentation
- Asia-Europe Symposium on Biomolecular Recognition, May 2003, EnglishDevelopment of a new SNP detectable method by use of the immobilized oligonucleotide capillary chromatographyOral presentation
- 化学工学会第68年会, Mar. 2003, Japanese緑藻Haematococcus pluvialisによるアスタキサンチン生産における光照射法の検討Oral presentation
- 化学工学会第68年会, Mar. 2003, Japanese二槽式バイオリアクターによる光合成微生物の生産性の向上Oral presentation
- Young Asian Biochemical Engineers' Community (YABEC) Symposium 2002, Nov. 2002, EnglishAstaxanthin production by Haematococcus pluvialis using LED lampsOral presentation
- 化学工学会第35回秋季大会, Sep. 2002, English緑藻Haematococcus pluvialisによるアスタキサンチン生産に及ぼす照射光の影響Oral presentation
- 化学工学会第35回秋季大会, Sep. 2002, Japanese発光ダイオードを用いた緑藻Haematococcus pluvialisによるアスタキサンチン生産Oral presentation
- 化学工学会第35回秋季大会, Sep. 2002, Japaneseタンパク質のリフォールディングにおよぼす変性剤除去の影響Oral presentation
- 化学工学会鳥取大会, Jul. 2002, Japanese高効率土壌汚染浄化法を利用した酸性雨の中和Oral presentation
- 化学工学会第67年会, Mar. 2002, Japanese光合成微生物培養液中における光強度減衰モデルの適用性Oral presentation
- 化学工学会第67年会, Mar. 2002, JapaneseB. subtilisの自己固定化能力を利用した酸性雨の土壌中での中和Oral presentation
- Young Asian Biochemical Engineers' Community (YABEC) Symposium 2001, Oct. 2001, EnglishLight attenuation in suspension of purple bacterium Rhodobacter capsulatus and green alga Chlamydomonas reinhardtiiOral presentation
- 日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 2001, Japanese光合成微生物培養液中における光強度減衰Oral presentation
- 化学工学会福井大会, Jul. 2001, Japanese光合成微生物培養液中での光強度減衰に及ぼす光源の影響Oral presentation
- 化学工学会第66年会, Apr. 2001, Japanese光合成微生物培養液中での光強度減衰Oral presentation
- 化学工学会第66年会, Apr. 2001, Japaneseテーラー渦型反応器によるタンパク質の連続リフォールディングOral presentation
- 化学工学会第32回秋季大会, Sep. 1999, Japanese円筒型光リアクターの新規な光強度分布モデルと光合成細菌による水素生産への適用Oral presentation
- 日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1999, Japanese円筒型フォトバイオリアクターの新規な光強度分布モデルと光合成微生物による水素生産への適用Oral presentation
- 日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1998, Japaneseエタノールアミンを窒素源として用いた光合成細菌Rhodobacter capsulatusによる水素生産Oral presentation
- 平成9年度日本生物工学会大会, Sep. 1997, Japaneseエタノールアミンを窒素源として用いた、Rhodobacter capsulatusによる水素生産Oral presentation
- 化学工学会第29回秋季大会, Sep. 1996, EnglishHydrogen detectable color indicator for screening of microorganismsPoster presentation
- European Federation of BiotechnologyMay 2007 - Present
- Asian Federation of Biotechnology
- 日本生物工学会
- 化学工学会
- 日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2021 - 31 Mar. 2024培養温度制御に基づく真核細胞への遺伝子導入の効率化本研究では,研究代表者らが緑藻の培養で見出した,培養温度を適切な範囲で周期的に変化させると培養液中の細胞間で細胞周期が同調するという知見に基づき,真核細胞の細胞周期を同調させることにより,核膜の消失から再構成の間にあわせて外来遺伝子を導入できるようにし,これによって外来遺伝子の核内移行,ならびに核DNAとの接触を促進させて,真核細胞への遺伝子導入の効率化を図ることを検討する. 令和3年度には,開放型の有糸分裂を行う昆虫細胞に注目し,培養温度をプログラム制御できるインキュベーターを用いて培養を行い,細胞周期が同調する温度制御条件の検討を行った.昆虫細胞には,組換えタンパク質の発現に広く利用されており,子宮頸がんワクチンの生産にも利用されているTrichoplusia ni BTI-TN-5B1-4 (High Five)細胞を用いた. はじめに,培養温度を最適よりも高温側で一定に保持して増殖挙動を調べた.High Five細胞は35℃までは最適温度である27℃のときと同等の増殖速度ならびに最高到達細胞密度を示した.しかし,37℃では増殖が顕著に抑制され,39℃では増殖しないことが分かった.培養液中のそれぞれの細胞の細胞周期をフローサイトメーターにより分析したところ,G2/M期にある細胞の全細胞数に対する割合が27℃の15-20%から37℃の30-40%まで,温度上昇に伴って増加することを見出した. そこで,培養温度は27℃と37℃を12 h周期で変化させることとし,High Five細胞の細胞周期の同調を試みた.しかし,植継ぎを行いながら6 dにわたって培養を継続したが,細胞周期が同調したときに見られる細胞密度の倍加は観察されなかった.High Five細胞では緑藻と同様にして細胞周期を同調させることは困難であることが分かった.
- 学術研究助成基金助成金/基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigatorCompetitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2015 - 31 Mar. 2019Production of influenza virus-like particles in recombinant insect cellsProduction of influenza virus-like particles in recombinant insect cells was investigated. The cDNA fragments encoding hemagglutinin (HA) and matrix protein 1 (M1) of an influenza virus A were separately cloned into the plasmid vector pIHAbla and pIHAneo. The pIHAbla and pIHAneo contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with either a blasticidin or neomycin resistance gene for use as a selectable marker, respectively. After cotransfection with the prepared plasmids, High Five cells were incubated with blasticidin and G418, and cells resistant to the antibiotics were effectively obtained. Sucrose density-gradient sedimentation analysis of the culture supernatant showed that secreted HA and M1 molecules were produced in a particulate form.
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2008 - 2010In order to investigate the formation mechanism of liposomes in a micro-channel, an experimental study has been performed. The Lagrangian observations and velocity field measurements by a micro-PTV were carried out by use of a movable stage. From the results, it was found that the lipid film pre-coated on the wall were taken into the liquid/air interface and the rod-like liposomes were formed between the interface and the wall and that the rod-like liposomes becomes spherical liposomes in a flow. It was also found that the rod-like liposomes serially induced from the interface by the strong reverse flow existing near the interfaceCompetitive research funding
- 2008, Principal investigator産業技術研究「活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発」Competitive research funding
- 2007, Principal investigator昇温溶出アフィニティークロマトグラフィーによる一塩基多型の検出Competitive research funding
- 2006, Principal investigator活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発Competitive research funding
- 2005, Principal investigator活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発Competitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2003 - 2004Integrate System for Creation, Detection and Production of Antibodies with Variety of CharacteristicsIn the fields of development of pharmaceuticals, medical diagnosis-analysis and bioprocesses, production of variety of antibodies, detection of the interactions between antibody and protein, screening methods of antibodies and effective production processes of antibodies are essential. In this work, we developed the simple and rapid methods to obtain antibody ligands for recognition and detection of target proteins, based on our recent researches on production of large libraries of antibodies, a high sensitivity detection method of proteins and effective production of antibodies by secretion from yeast cells. 1.A colony lift assay for detection of antibody secreted from E. coli was developed by use of antigen-adsorbed PVDF membrane and liposome immunoblotting assay. By use of this method, high density and micro-assay was possible and the number of samples treated was increased. 2.By use of HRP encapsulating immunoliposomes and hydrophobic membranes, an immunoblotting assay method with two orders of magnitude higher sensitivity, compared with the conventional immunoblotting assay using HRP-conjugated antibody, was developed. 3.A large library of whole antibodies was obtained based on mating of yeast cells transformed by libraries of H- and L-chains, and aproduction method of antibodies by secretion from yeast cells was established. 4.An affinity biopanning method utilizing immunoliposomes was developed and it was applied to biopanning selection with a high efficiency.