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KATSUDA Tomohisa
Graduate School of Engineering / Department of Chemical Science and Engineering
Associate Professor

Researcher basic information

■ Research Keyword
  • Biochemical Engineering
  • 微細藻類
  • クロマトグラフィー
■ Research Areas
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering
■ Committee History
  • Apr. 2017 - Mar. 2019, The Society of Chemical Engineers, Japan, Member, Kansai Branch
  • Mar. 2012 - Feb. 2018, The Society of Chemical Engineers, Japan, Secretary, Division of Biochemical Engineering
  • Jun. 2003 - Dec. 2011, The Society for Biotechnology, Member, Kansai Branch
  • Apr. 2002 - Oct. 2011, Young Asian Biochemical Engineers’ Community, 日本事務局員
  • Oct. 2002 - Dec. 2010, 日本生物工学会関西支部, バイオテクノロジー実験講座講師
  • Apr. 2000 - Mar. 2009, 化学工学会関西支部CES21, 運営委員

Research activity information

■ Award
  • Sep. 2024 The Society of Chemical Engineers, Japan, Outstanding Paper Award of 2023, Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix
    Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji
    Official journal

  • Nov. 2023 神戸大学工学部, 令和四年度優秀教育賞(応用化学科)
    勝田知尚

  • Nov. 2018 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of influenza virus-like particles in stably transformed insect cells
    MATSUDA Takuya, TANIJIMA Toshikazu, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa, YAMAJI Hideki
    International society

  • Oct. 2018 神戸大学工学部, 平成29年度優秀教育賞(応用化学科)
    勝田 知尚

  • Sep. 2017 化学工学会, バイオ部会優秀ポスター賞, 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    Japan society

  • Nov. 2016 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of virus-like particles using recombinant insect cells
    TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki
    International society

  • Oct. 2010 Asia Pacific Confederation of Chemical Engineering, Best Poster Paper Award (The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010)), Production of Japanese encephalitis virus-like particles in insect cell expression systems
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    International society

  • Feb. 2010 化学工学会関西支部, 化学工学会バイオ部会, 第8回最先端バイオテクノロジー発表会ポスター賞, 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響, 野上達弘
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  • Dec. 2009 Young Asian Biochemical Engineers’ Community, Best Poster Presentation Award for an Original Contribution to the 15th Symposium of Young Asian Biochemical Engineers’ Community, Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli
    KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  • Sep. 2007 神戸大学工学部, 平成18年度優秀教育賞(移動現象論・分離工学分野)
    大村 直人, 今駒 博信, 鈴木 洋, 勝田 知尚

  • Sep. 2007 日本生物工学会, 生物工学奨励賞(照井賞), フォトバイオリアクターによる有用物質生産のための生物化学工学的検討
    KATSUDA Tomohisa

  • Mar. 2005 化学工学会, Masaki Naito Memorial Award, 光合成微生物による光培養生産プロセスの開発
    KATSUDA Tomohisa

■ Paper
■ MISC
  • CCSと微細藻類による有用物質生産
    勝田知尚
    Feb. 2008, 生物工学会誌, 86(2) (2), 84 - 84, Japanese
    Introduction scientific journal

  • 光合成微生物による水素生産のゆくえ
    勝田知尚
    日本生物工学会, Mar. 2002, 生物工学会誌, 80(3) (3), 128 - 128, Japanese
    Introduction scientific journal

■ Books And Other Publications
  • Production of antibody fragments in Escherichia coli. In: Patrick Chames (ed.), Antibody Engineering: Methods and Protocols, Second Edition, Method in Molecular Biology, vol. 907, pp. 305-324 (Chapter 18)
    KATSUDA Tomohisa, SONODA Hiroyuki, KUMADA Yoichi, YAMAJI Hideki
    Others, Springer Science+Business Media, Aug. 2012, English
    Scholarly book

  • 抗体医薬のための細胞構築と培養技術
    KATSUDA Tomohisa
    Others, CMC Publishing, Jun. 2010, Japanese
    Scholarly book

  • Frontier of Eco-Bio Energy
    KATSUDA Tomohisa, OOSHIMA Hiroshi
    Joint work, シーエムシー出版, Sep. 2005, Japanese
    Scholarly book

■ Lectures, oral presentations, etc.
  • Using Confocal Laser Scanning Microscopy to Analyze the Mass Transfer in the Protein A Medium of a Silica Matrix
    Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji
    SCEJ 55th Autumn Meeting, Sep. 2024, English, Sapporo
    [Invited]
    Invited oral presentation

  • Efficient transfection of insect cells with controlling cell cycle progression
    Hikaru Mori, Ryo Iwanaga, Yuki Honjo, Hideki Yamaji, Tomohisa Katsuda
    SCEJ 89th Annual Meeting, Mar. 2024, Japanese
    Poster presentation

  • Production of influenza virus-like particles displaying heterologous proteins
    Shimizu K, Matsuda T, Katsuda T, Yamaji H
    化学工学会第54回秋季大会, Sep. 2023, English
    Poster presentation

  • 昆虫細胞を用いたキメラインフルエンザウイルス様粒子の生産
    清水 康平, 市川 祐大, 松田 拓也, 勝田 知尚, 山地 秀樹
    化学工学会第88年会, Mar. 2023
    Poster presentation

  • 培養温度と光強度を概日周期で制御したヘマトコッカス プルビアリスによるアスタキサンチン生産
    勝田 知尚, 奥村 魁, 山地 秀樹
    化学工学会第88年会, Mar. 2023
    Poster presentation

  • An investigation of diffusion and adsorption behavior in protein A media
    Kohei HAYASHI, Tomohisa KATSUDA, Hideki YAMAJI
    化学工学会第87年会, Mar. 2022, English
    Poster presentation

  • 異種タンパク質を提示するインフルエンザウイルス様粒子の昆虫細胞を用いた生産
    市川 裕大, 松田 拓也, Prihardi Kahar, 勝田 知尚, 山地 秀樹
    化学工学会第87年会, Mar. 2022, English
    Poster presentation

  • 分離精製(膜・クロマト分離)
    勝田 知尚
    日本生物工学会100周年記念事業教育セミナー「培養技術勉強会」, Nov. 2021, Japanese
    [Invited]
    Public discourse

  • 遺伝子組換え昆虫細胞樹立へのゲノム編集技術の応用
    松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹
    化学工学会第52回秋季大会, Sep. 2021

  • ゲノム編集技術を用いた遺伝子組換え昆虫細胞の樹立
    松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹
    第34回日本動物細胞工学会2021年度大会 (JAACT2021), Jul. 2021

  • プロテインA固定化マイクロアフィニティカラムを用いた疑似オンラインIgG濃度測定法の開発
    川村 和樹, 勝田 知尚, 山地 秀樹
    化学工学会第86年会, Mar. 2021, Japanese
    Poster presentation

  • Production of influenza virus-like particles using recombinant insect cells
    Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    The 33rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2020 Fuchu), Nov. 2020

  • 昆虫細胞を用いたインフルエンザウイルス様粒子の生産
    松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹
    化学工学会第51回秋季大会, Sep. 2020, Japanese

  • Effects of the circadian control in temperature and light intensity on the cell division of green algae under a photoautotrophic condition
    Tomohisa KATSUDA, Hiroyuki YAMADA, Takaaki MORIMOTO, Hitoki KUWAI, Hideki YAMAJI
    Young Asian Biological Engineers' Community Symposium 2019, Nov. 2019, English, Young Asian Biological Engineers' Community, Seoul, Korea, Republic of
    Poster presentation

  • Adsorption of immunoglobulin G in protein A resins studied by confocal laser scanning microscopy taking account of the optical hindrance inside a particle
    Tomohisa Katsuda, Tomoki Yamaguchi, Naruaki Inoue, Yuki Kase, Kou Ota, Hideki Yamaj
    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English
    Poster presentation

  • Production of influenza virus proteins in recombinant insect cells
    Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sep. 2019, English
    Poster presentation

  • 微細藻の細胞分裂に及ぼす培養温度・光強度の周期的制御の影響
    勝田 知尚, 森本 高章, 桑井 仁葵, 山地 秀樹
    第71回日本生物工学会大会, Sep. 2019, Japanese
    Oral presentation

  • 組換え昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産
    松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹
    第32回日本動物細胞工学会2019年度大会 (JAACT2019), Jul. 2019, Japanese
    Poster presentation

  • Production of influenza virus-like particles in insect cells
    Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda
    26th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2019), May 2019, English
    Poster presentation

  • 固定化したシトクロムP450発現大腸菌によるwhole cell bioconversion
    FUJII ATSUSHI, ISHISAKA Akiho, KATSUDA Tomohisa, IMAISHI Hiromasa, YAMAJI Hideki
    化学工学会第84年会, Mar. 2019, Japanese, 化学工学会, 東京, Domestic conference
    Poster presentation

  • Production of influenza virus-like particles in stably transformed insect cells
    MATSUDA Takuya, TANIJIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, 日本動物細胞工学会, 筑波, International conference
    Poster presentation

  • Production of antibody Fab fragment using 2A peptide in insect cells
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, 日本動物細胞工学会, 筑波, International conference
    Poster presentation

  • 緑藻Chlorella sorokinianaの光独立栄養培養における培養温度の影響
    KUWAI Hitoki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第50回秋季大会, Sep. 2018, Japanese, 化学工学会, 鹿児島, Domestic conference
    Poster presentation

  • 培養温度・光強度の周期的な変化が緑藻Haematococcus pluvialisの細胞分裂に及ぼす影響
    MORIMOTO Takaaki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第50回秋季大会, Sep. 2018, Japanese, 化学工学会, 鹿児島, Domestic conference
    Poster presentation

  • 昆虫細胞における2Aペプチドを用いた抗体生産
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会大会, 吹田, Domestic conference
    Oral presentation

  • ゲノム編集技術を用いた組換え昆虫細胞の作製
    TOGAMI Shinya, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第50回秋季大会, Sep. 2018, English, 化学工学会, 鹿児島, Domestic conference
    Poster presentation

  • Production of influenza virus proteins in stably transformed insect cells
    YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa
    18th European Congress on Biotechnology (ECB2018), Jul. 2018, English, European Federation of Biotechnology, Geneva, Switzerland, International conference
    Poster presentation

  • 緑藻Haematococcus pluvialisにおける周期的温度変化と細胞増殖の関係
    KATSUDA Tomohisa, MORIMOTO Takaaki, YAMAJI Hideki
    化学工学会第83年会, Mar. 2018, Japanese, 化学工学会, 吹田, Domestic conference
    Poster presentation

  • 昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産
    MATSUDA Takuya, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第83年会, Mar. 2018, Japanese, 化学工学会, 吹田, Domestic conference
    Poster presentation

  • 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産
    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • 共焦点レーザー走査顕微鏡観察と数値ミュレーションに基づく抗体の吸着・破過の解析
    HIROOKA Kanamu, YAMAGUCHI Tomoki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • エアリフト式フォトバイオリアクターの操作条件と微細藻類の光独立栄養増殖の関係
    KUREBAYASHI Aika, MORIMOTO Takaaki, NISHIMURA Takumi, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第49回秋季大会, Sep. 2017, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • Production of influenza virus proteins by recombinant insect cells
    YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa
    International Meeting & 42nd Annual Conference of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Aug. 2017, English, The Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Kuala Lumpur, Malaysia, International conference
    Poster presentation

  • 昆虫細胞を用いたインフルエンザウイルスタンパク質の生産
    YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa
    第30回日本動物細胞工学会2017年度大会 (JAACT2017), Jul. 2017, Japanese, 日本動物細胞工学会, 松山, Domestic conference
    Poster presentation

  • Efficient protein production by transient gene expression using insect cells
    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa
    25th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2017), May 2017, English, European Society for Animal Cell Technology (ESACT), Lausanne, Switzerland, International conference
    Poster presentation

  • Transient gene expression in insect cells for efficient recombinant antibody production
    TAKAHASHI Ryoma, MASUMI-KOIZUMI Kyoto, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conference
    Poster presentation

  • Production of virus-like particles using recombinant insect cells
    TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki
    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conference
    Poster presentation

  • Characterization of the diffusion and adsorption of IgG in protein A-coupled adsorbents with confocal laser scanning microscopy
    HIROOKA Kanamu, HARUNA Yusuke, YAMAJI Hideki, KATSUDA Tomohisa
    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), Nov. 2016, English, 日本動物細胞工学会, 神戸, International conference
    Poster presentation

  • Efficient antibody production by transient gene expression in insect cells
    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa
    17th European Congress on Biotechnology (ECB2016), Jul. 2016, English, European Federation of Biotechnology (EFB), Krakow, Poland, International conference
    Poster presentation

  • 培養条件の概日変化が緑藻Haematococcus pluvialisの栄養増殖に及ぼす影響
    Kohei YOSHIMINE, YAMADA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第81年会, Mar. 2016, Japanese, 化学工学会, 大阪, Domestic conference
    Poster presentation

  • 共焦点レーザー走査顕微鏡観察に基づくProtein A固定化吸着剤の拡散・吸着特性の評価
    KATSUDA Tomohisa, HARUNA Yusuke, HIROOKA Kanamu, KASE Yuki, OTA Kou, YOSHIKAWA Toru, YAMAJI Hideki
    化学工学会第81年会, Mar. 2016, Japanese, 化学工学会, 大阪, Domestic conference
    Oral presentation

  • Recombinant antibody production by transient gene expression in insect cells
    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa
    Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conference
    Poster presentation

  • Photoautotrophic growth of Haematococcus pluvialis under controlled temperature and light conditions
    KATSUDA Tomohisa, GIANNELLI Luca, YAMADA Hiroyuki, YAMAJI Hideki
    Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conference
    Poster presentation

  • Optimization of microalgal cultivation in cascade photobioreactors with wavy bottom via computational fluid dynamics
    WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa
    Asian Congress on Biotechnology 2015 (ACB2015), Nov. 2015, English, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, Domestic conference
    Poster presentation

  • シトクロムP450発現大腸菌を用いたwhole cell bioconversionに及ぼす培養・反応条件の影響
    HARADA Masayuki, MORINAKA Ryohei, KATSUDA Tomohisa, IMAISHI Hiromaisa, YAMAJI Hideki
    化学工学会第47回秋季大会, Sep. 2015, Japanese, 化学工学会, 札幌, Domestic conference
    Poster presentation

  • 昆虫細胞を宿主とした一過性発現による抗体タンパク質の生産
    MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    第28回日本動物細胞工学会2015年度大会 (JAACT2015), Jul. 2015, Japanese, 日本動物細胞工学会, 仙台, Domestic conference
    Poster presentation

  • Production of an antibody molecule by transient gene expression in insect cells
    MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    24th ESACT Meeting Barcelona 2015, Jun. 2015, English, European Society for Animal Cell Technology (ESACT), Barcelona, Spain, International conference
    Poster presentation

  • 鉛に汚染された土壌の評価とバイオレメディエーションによる鉛の回収
    塩見 尚史, SUETSUGU KENICHIRO, KATSUDA TOMOHISA
    日本LCA学会, Mar. 2015, Japanese, Domestic conference
    Oral presentation

  • カスケード型フォトバイオリアクターにおける微細藻類培養の数値流体力学に基づく検討
    WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会姫路大会, Dec. 2014, Japanese, 化学工学会関西支部, 姫路, Domestic conference
    Poster presentation

  • Transient gene expression in insect cells for recombinant antibody production
    MORI Keita, HAMADA Hirotsugu, OGAWA Takahumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    The 27th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT2014 Kitakyushu), Nov. 2014, English, 日本動物細胞工学会, 北九州, International conference
    Poster presentation

  • 昆虫細胞を宿主とした一過性発現による抗体タンパク質生産
    HAMADA Hirotsugu, MORI Keita, OGAWA Takafumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第46回秋季大会, Sep. 2014, Japanese, 化学工学会, 福岡, Domestic conference
    Poster presentation

  • Production of antibody fragments by transient gene expression in insect cells
    YAMAJI Hideki, KATSUDA Tomohisa, HAMADA Hirotsugu, MORI Keita
    16th European Congress on Biotechnology (ECB16), Jul. 2014, English, European Federation of Biotechnology, Edinburgh, UK, International conference
    Poster presentation

  • 昆虫細胞を用いた一過性発現による機能性抗体タンパク質の生産
    MORI Keita, HAMAMOTO Kohei, MIYAGUCHI Naoko, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference
    Poster presentation

  • Haematococcus pluvialisの増殖とアスタキサンチン生産に及ぼす培養温度の影響
    YAMADA Hiroyuki, WADA Sotaro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference
    Poster presentation

  • Photobioreactor design using CFD: mixing and light effects on microalgal cultures
    GIANNELLI Luca, WADA Sotaro, YAMADA Hiroyuki, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第78年会, Mar. 2013, English, 化学工学会, 豊中市, Domestic conference
    Oral presentation

  • Production of recombinant antibody molecules in insect cells
    MIYAGUCHI Naoko, HAMAMOTO Kouhei, OGAWA Takafumi, SONODA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki
    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, English, 日本動物細胞工学会, 名古屋市, International conference
    Poster presentation

  • Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells
    HOTTA Yuri, MINAMITANI Azusa, NARITA Kazuma, KATSUDA Tomohisa, YAMAJI Hideki
    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), Nov. 2012, English, 日本動物細胞工学会, 名古屋市, International conference
    Poster presentation

  • 組換え昆虫細胞によるscFv-Fc融合タンパク質の生産
    SONODA Hiroyuki, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki
    第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference
    Oral presentation

  • Computational fluid dynamics for enhancing the light distribution in a photobioreactor grown culture of Hematococcus pluvialis
    GIANNELLI Luca, WADA Sotaro, YAMAJI Hideki, KATSUDA Tomohisa
    第64回日本生物工学会大会, Oct. 2012, English, 日本生物工学会, 神戸市, Domestic conference
    Oral presentation

  • 大腸菌を用いた一本鎖抗体生産における終止コドンの影響
    YAMAJO Yuki, FUKUMARU Yuka, YAMAJI Hideki, KATSUDA Tomohisa
    化学工学会第44回秋季大会, Sep. 2012, Japanese, 化学工学会, 仙台市, Domestic conference
    Poster presentation

  • 気液界面を利用した高収率リポソーム生成法の開発
    Hirohiko AIHARA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Ruri HIDEMA
    化学工学会第44回秋季大会仙台, Sep. 2012, Japanese, 仙台, Domestic conference
    Oral presentation

  • 昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産
    YAMAJI Hideki, SEGAWA Maiko, NAKAMURA Masataka, KATSUDA Tomohisa
    化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 東京, Domestic conference
    Oral presentation

  • 炭化水素資化酵母Yarrowia lipolyticaによる油汚染土壌の修復のための無機栄養源の開発
    FUJIMOTO Sho, TANUMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • 昆虫細胞を用いた抗体タンパク質の分泌生産
    HAMAMOTO Kouhei, MIYAGUCHI Naoko, SAWAMOTO Shiori, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • 昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討
    NARITA Kazuma, HOTTA Yuri, MINAMITANI Azusa, NAGASUGA Takashi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • シャイン・ダルガーノ配列を改変した大腸菌による一本鎖抗体の流加培養生産
    FUKUMARU Yuka, NOGAMI Tatsuhiro, KIHARA Mana, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋, Domestic conference
    Poster presentation

  • Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    IUMS 2011 Sapporo Congress, Sep. 2011, English, International Union of Microbiological Societies, 札幌, Domestic conference
    Poster presentation

  • Secretory production of virus-like particles by recombinant insect cells
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    ESACT 2011 Meeting, May 2011, English, European Society for Animal Cell Technology, Vienna, Austria, International conference
    Poster presentation

  • 炭化水素資化酵母Yarrowia lipolyticaを用いた油汚染土壌の修復における栄養源の添加条件の検討
    FUJIMOTO Shou, TANUMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki
    第13回化学工学会学生発表会(神戸大会), Mar. 2011, Japanese, 化学工学会, 神戸市, Domestic conference
    Oral presentation

  • 大腸菌を用いた一本鎖抗体生産におけるシャイン・ダルガーノ配列の影響
    FUKUMARU Yuka, NOGAMI Tatsuhiro, KATSUDA Tomohisa, YAMAJI Hideki
    第13回化学工学会学生発表会(神戸大会), Mar. 2011, Japanese, 化学工学会, 神戸市, Domestic conference
    Oral presentation

  • 大腸菌におけるインクルージョンボディ生成に及ぼすシャイン・ダルガーノ配列の影響
    KATSUDA Tomohisa, FUKUMARU Yuka, KIHARA Mana, NOGAMI Tatsuhiro, YAMAJI Hideki
    化学工学会第76年会, Mar. 2011, Japanese, 化学工学会, 東京都, Domestic conference
    Oral presentation

  • リポソームの応用事例
    KATSUDA Tomohisa
    近畿バイオインダストリー振興会議第5回フォローアップ勉強会, Jan. 2011, Japanese, 近畿バイオインダストリー振興会議, 大阪市, Domestic conference
    Invited oral presentation

  • Optimized Shine-Dalgarno sequence for efficient production of single-chain Fv antibody in Escherichia coli
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), Dec. 2010, English, Canadian Society for Chemistry, American Chemical Society, Chemical Society of Japan, New Zealand Institute of Chemistry, Royal Australian Chemical Institute, Korean Chemical Society, Chinese Chemical Society, Honolulu, USA, International conference
    Poster presentation

  • Rapid production of functional antibody molecules by transient gene expression in insect cells
    SAWAMOTO Shiori, HAMAMOTO Kouhei, KATSUDA Tomohisa, YAMAJI Hideki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, English, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, International conference
    Poster presentation

  • Effect of signal peptide on the production of single-chain fv antibody by Escherichia coli
    NOGAMI Tatsuhiro, FUKUMARU Yuka, KATSUDA Tomohisa, YAMAJI Hideki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, Nov. 2010, English, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, International conference
    Poster presentation

  • Production of Japanese encephalitis virus-like particles in insect cell expression systems
    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010), Oct. 2010, English, Asia Pacific Confederation of Chemical Engineering, Taipei, Taiwan, International conference
    Poster presentation

  • マイクロ流路内におけるリポソーム生成時のマイクロ流体挙動に関する研究
    FUJIWARA Jun, SUZUKI Hiroshi, KOMODA Hiroyuki, KATSUDA Tomohisa
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市
    Oral presentation

  • プロテインAアフィニティクロマトグラフィーのための溶離条件検討法の開発
    KASE Yuki, NISHIYAMA Takuya, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conference
    Poster presentation

  • シャペロン共発現による大腸菌での一本鎖抗体 (scFv) 融合タンパク質の生産
    SONODA Hiroyuki, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conference
    Poster presentation

  • アルカン資化酵母Yarrowia lipolyticaを用いた土壌汚染油の分解
    TANUMA Naoki, FUJIMOTO Shou, SHIOMI Naofumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第42回秋季大会, Sep. 2010, Japanese, 化学工学会, 京都市, Domestic conference
    Poster presentation

  • Production of Japanese encephalitis virus-like particles using insect cells
    NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, YAMAJI Hideki
    The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010), Sep. 2010, English, The Japanese Association for Animal Cell Technology, 札幌市, International conference
    Poster presentation

  • Pichia pastorisを用いた一本鎖抗体生産における誘導初期のメタノール濃度の影響
    FUJITA Keisuke, AKIYAMA Naoki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第75年会, Mar. 2010, Japanese, 化学工学会, 鹿児島, Domestic conference
    Oral presentation

  • 昆虫細胞-バキュロウイルス系を用いた抗体タンパク質の高効率生産
    SAWAMOTO Shiori, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki
    第8回最先端バイオテクノロジー発表会, Feb. 2010, Japanese, 化学工学会, 大阪, Domestic conference
    Poster presentation

  • 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    第8回最先端バイオテクノロジー発表会, Feb. 2010, Japanese, 化学工学会, 大阪, Domestic conference
    Poster presentation

  • Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli
    KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2009, Dec. 2009, English, Young Asian Biochemical Engineers Community, Xiamen, International conference
    Poster presentation

  • Production of Japanese encephalitis virus-like particles by recombinant insect cells
    YAMAJI Hideki, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji
    Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC '09), Nov. 2009, English, APBioChEC '09 Committee, 神戸市, International conference
    Oral presentation

  • Oil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolytica
    FUKUI Mayu, SHIOMI Shinji, TANUMA Naoki, KATSUDA Tomohisa, SHIOMI Naofumi, YAMAJI Hideki
    The 9th Asia-Pacific Biochemical Engineering Conference 2009, Nov. 2009, English, The 9th Asia-Pacific Biochemical Engineering Conference 2009, Kobe, International conference
    Poster presentation

  • 大腸菌を用いた一本鎖抗体生産におけるSD配列の影響
    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference
    Poster presentation

  • 昆虫細胞を用いた日本脳炎ウイルス様粒子の生産
    TAKAHASHI Yusuke, NAGASUGA Takashi, NAKAMURA Masataka, KATSUDA Tomohisa, YAMAJI Hideki
    第61回日本生物工学会大会, Sep. 2009, Japanese, 日本生物工学会, 名古屋市, Domestic conference
    Oral presentation

  • 昆虫細胞-バキュロウイルス系による抗体タンパク質の生産
    SAWAMOTO Shiori, SUZUKI Tasuku, FURUTA Takanori, OGAWA Takafumi, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference
    Poster presentation

  • ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用
    IWASAKI Daiki, SENGA Itaru, YAMASHITA Takashi, TONAI Masahiro, OGINO Chiaki, SHIMIZU Nobuaki, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference
    Poster presentation

  • 昆虫細胞を用いた日本脳炎ウイルスタンパク質の生産
    TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki
    化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference
    Oral presentation

  • 一塩基変異を検出する昇温溶出アフィニティクロマトグラフィーに対するPCRの適用性
    KATSUDA Tomohisa, IMANISHI Makoto, KATOH Shigeo, YAMAJI Hideki
    化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference
    Oral presentation

  • マイクロ流路内におけるリポソーム生成機構
    FUJIWARA Jun, HAMAMURA Junya, SUZUKI Hiroshi, KATSUDA Tomohisa, KOMODA Hiroyuki, USUI Hiromoto
    化学工学会第74年会, Mar. 2009, 化学工学会, 横浜, Domestic conference
    Oral presentation

  • Production of adenovirus vector by 293 cells immobilized within porous biomass support particles
    MORISHITA Naoya, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki
    The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, English, 日本動物細胞工学会, 福岡市, International conference
    Poster presentation

  • Low multiplicity infection of 293 cells for adenovirus vector production
    MORISHITA Naoya, YAMADA Kentaro, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki
    The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, Nov. 2008, English, 日本動物細胞工学会, 福岡市, International conference
    Poster presentation

  • High efficiency expression of soluble scFv in E. coli by use of linkers containing rare codons
    KAJIHARA Hideyuki, SAKAN Yoshinobu, KIHARA Mana, KIKUCHI Yasufumi, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki, KATOH Shigeo
    The 14th Symposium of Young Asian Biochemical Engineers’ Community, Nov. 2008, English, Young Asian Biochemical Engineers’ Community, 東京都, International conference
    Poster presentation

  • Effects of the parameters of flashing light from blue emitting diodes on astaxanthin production by H. pluvialis
    SENGA Itaru, SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2008, Nov. 2008, English, Young Asian Biochemical Engineers Community, Tokyo, International conference
    Poster presentation

  • A biochemical engineering study of photo-bioproces
    KATSUDA Tomohisa
    The 2 nd SCEJ (Kansai-Branch)/SSCCI Joint International Conference on Chemical Engineering, Nov. 2008, English, SCEJ (Kansai-Branch) and SSCCI, Shanghai, China, International conference
    [Invited]
    Invited oral presentation

  • Secretory production of Japanese encephalitis virus E protein by recombinant insect cells
    TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki
    “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, English, Hanyang University, Ansan, Korea, Domestic conference
    Poster presentation

  • Detection of single nucleotide variation in amplified gene fragments using oligonucleotide-coupled affinity column
    KATSUDA Tomohisa, IMANISHI Makoto, YAMAJI Hideki, KATOH Shigeo
    “Bioseparation for Biorecognition and Bionanotechnology” Conference, Oct. 2008, English, Hanyang University, Ansan, Korea, Domestic conference
    Poster presentation

  • 多孔性粒子に固定化した大腸菌を用いる有用物質生産
    JIN Na, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki
    化学工学会第40回秋季大会, Sep. 2008, Japanese, 化学工学会, 仙台市, Domestic conference
    Poster presentation

  • レアコドンリンカーによる大腸菌での可溶性scFvの高効率生産
    KIHARA Mana, SAKAN Yoshinobu, KAJIHARA Hideyuki, AKASAKA Sho, KATSUDA Tomohisa, KATOH Shigeo, YAMAJI Hideki
    化学工学会第40回秋季大会, Sep. 2008, Japanese, 化学工学会, 仙台市, Domestic conference
    Poster presentation

  • 微生物の光合成を利用した有用物質生産ーエネルギーと食品への活用ー
    勝田 知尚
    第108回微小光学研究会, Jul. 2008, Japanese
    [Invited]
    Public discourse

  • Liposome Formation Characteristics with a Micro-Reactor
    Jun-ya HAMAMURA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Shigeo KATO, Hiromoto USUI
    11th Liposome Research Days Conference, Jul. 2008, English, Yokohama, Japan, International conference
    Oral presentation

  • Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen species
    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Daiki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    American Society for Microbiology 108th General Meeting, Jun. 2008, English, American Society for Microbiology, Boston, USA, International conference
    Poster presentation

  • マイクロ流路を用いたリポソームの生成機構
    鈴木 洋, 浜村 隼矢, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基
    化学工学会 第73年会, Mar. 2008, Japanese, 静岡, Domestic conference
    Oral presentation

  • Enhancement of growth and astaxanthin production of Haematococcus pluvialis by using airlift photobioreactor
    Ranjbar Reza, Inoue Ryota, Iwasaki Daiki, Katsuda Tomohisa, Katoh Shigeo
    化学工学会第73年会, Mar. 2008, English, 化学工学会, 浜松, Domestic conference
    Oral presentation

  • ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化
    SENGA Itaru, YAMAGAMI Keisuke, KATSUDA Tomohisa, OGINO Chiaki, SHIMIZU Nobuaki, KATOH Shigeo
    第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference
    Oral presentation

  • シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価
    TAKEDA Naoki, KATSUDA Tomohisa, KATOH Shigeo, MIYAHARA Hiroyoshi, INOUE Masaki, NAKAMURA Shuji
    第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference
    Oral presentation

  • High efficiency production of astaxantin by autotrophic cultivation of Haematococcua pluvialis in column photobioreactors
    RANJBAR Reza, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo
    第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, English, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference
    Oral presentation

  • Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination
    SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo
    上海市化学化工学会・化学工学会関西支部 第1回若手研究交流会, Dec. 2007, English, 上海市化学化工学会、化学工学会関西支部, 大阪, International conference
    Poster presentation

  • 青色点滅光照射下でのH. pluvialisの培養に及ぼす光強度および周波数の影響
    SHIRAISHI Hironori, ISHIZU Naoki, SENGA Itaru, RANJBAR Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会山口大会, Nov. 2007, Japanese, 化学工学会中国四国支部, 宇部, Domestic conference
    Poster presentation

  • Application of PCR to Affinity Chromatography for SNP Detection
    KATSUDA Tomohisa, KATOH Shigeo
    The 6th Asia-Europe Biorecognition Engineering Society Conference, Nov. 2007, English, Asia-Europe Biorecognition Engineering Society, Taoyuan, Taiwan, International conference
    [Invited]
    Invited oral presentation

  • Oxidative treatment of H. pluvialis with the sonocatalytic reaction
    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Hiroki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conference
    Poster presentation

  • Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination
    SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conference
    Poster presentation

  • A Biochemical Engineering Study of Bioproductions using Photobioreactor
    KATSUDA Tomohisa
    2007 Fall KSBB Meeting & International Symposium, Oct. 2007, English, The Korean Society for Biotechnology and Bioengineering, Daegu, Korea, International conference
    [Invited]
    Invited oral presentation

  • 二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導
    KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference
    Oral presentation

  • 気泡塔型フォトバイオリアクターを用いたヘマトコッカス藻による効率的アスタキサンチン生産
    INOUE Ryota, Ranjbar Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第39回秋季大会, Sep. 2007, Japanese, 化学工学会, 札幌, Domestic conference
    Poster presentation

  • マイクロキャピラリー内におけるリポソーム生成特性に関する研究
    濱村 隼矢, 鈴木 洋, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基
    化学工学会 第39回秋季大会, Sep. 2007, Japanese, 北海道大学, Domestic conference
    Oral presentation

  • フォトバイオリアクターによる 有用物質生産のための生物化学工学的検討
    KATSUDA Tomohisa
    第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference
    [Invited]
    Others

  • Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasounds
    KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki
    13th European Congress on Biotechnology, Sep. 2007, English, European Federation of Biotechnology, Barcelona, Spain, International conference
    Poster presentation

  • DNAアフィニティカラムを用いるSNP検出法へのPCR増幅法の応用
    IMANISHI Makoto, NISHIWADA Yasushi, FUKUI Mayu, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第39回秋季大会, Sep. 2007, Japanese, 化学工学会, 札幌, Domestic conference
    Poster presentation

  • 青色点滅光照射下のH. pluvialisにおけるアスタキサンチン生産量と光強度との関係
    SHIRAISHI Hironori, YAMAGAMI Keisuke, RANJBAR Reza, SENGA Itaru, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference
    Oral presentation

  • メタノール資化性酵母を用いた一本鎖抗体生産におけるメタノール供給方法
    OHNISHI Yuka, YAMAWAKI Shinya, MATSUMOTO Takehiro, SHIOMI Naofumi, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference
    Oral presentation

  • シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価
    TAKEDA Naoki, IMADA Masami, KATSUDA Tomohisa, KATOH Shigeo, MIYAHARA Hiroyoshi, INOUE Masaki, NAKAMURA Shuji
    化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference
    Oral presentation

  • Optimization of silica based media for antibody purification by protein A affinity chromatography
    IMADA Masami, TAKEDA Naoki, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, English, Young Asian Biochemical Engineers Community, Kaohsiung, International conference
    Poster presentation

  • Effects of flashing light from blue LEDs on the cell growth and astaxanthin production of Haematococcus pluvialis
    YAMAGAMI Keisuke, SHIRAISHI Hironori, RANJBARDAVIJANI Reza, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2006, Nov. 2006, English, Young Asian Biochemical Engineers Community, Kaohsiung, International conference
    Poster presentation

  • Optimization of silica-based matrices for antibody purification by protein A affinity chromatography
    KATOH Shigeo, IMADA Masami, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji
    26th international symposium on the separation of proteins, peptides and polynucleotides, Oct. 2006, English, ISPPP, Innsbruck, International conference
    Oral presentation

  • 昇温溶出アフィニティークロマトグラフィーによる一塩基多型検出法の開発
    KATSUDA Tomohisa
    化学工学会バイオ部会員フォーマルセミナー, Sep. 2006, Japanese, 化学工学会バイオ部会, 福岡, Domestic conference
    Oral presentation

  • メタノール資化性酵母Pichia pastorisの連続培養による一本鎖抗体生産
    YAMAWAKI Shinya, MATSUMOTO takehiro, OHNISHI Yuka, KATSUDA Tomohisa, SHIOMI Naofumi, KATOH Shigeo
    第58回生物工学会大会, Sep. 2006, Japanese, 生物工学会, 大阪, Domestic conference
    Oral presentation

  • ヘマトコッカスによるアスタキサンチン生産における青色点滅光照射の影響
    KATSUDA Tomohisa, SHIRAISHI Hironori, YAMAGAMI Keisuke, SENGA Itaru, SHIMAHARA Kazumichi, KATOH Shigeo
    第58回生物工学会大会, Sep. 2006, Japanese, 生物工学会, 大阪, Domestic conference
    Oral presentation

  • ヘマトコッカスによるアスタキサンチン生産における青色パルス光照射条件の検討
    SHIRAISHI Hironori, YAMAGAMI Keisuke, RANJBAR Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference
    Oral presentation

  • Pseudomonas sp. を用いる自己固定化バイオレメディエーションによるシアヌル酸分解
    SHIOMI Naofumi, ARII Kenji, NAKAI Hiroaki, YAMAGUCHI Yutaka, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference
    Oral presentation

  • Photocultivation of Haematococcus pluvialis Using Multi-Compartment Photobioreactor
    YOSHIMURA Satoshi, INOUE Ryota, RANJBER Reza, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conference
    Oral presentation

  • SNP Detection Method Using Temperature Gradient Affinity Chromatography
    NISHIWADA Yasushi, KAMURA Mitsumasa, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conference
    Oral presentation

  • Flashing light Illumination for Effective Production of Astaxanthin by Haematococcus pluvialis
    SHIMAHARA Kazumichi, KATSUDA Tomohisa, YAMAGAMI Keisuke, SHIRAISHI Hironori, KATOH Shigeo
    化学工学会第71年会, Mar. 2006, English, 化学工学会, 東京, Domestic conference
    Oral presentation

  • Effects of Iron Ion Concentration on the Growth Stability of Photosynthetic Bacteria in the Semi-Continuous Cultivation
    KATSUDA Tomohisa, YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, INOUE Ryota, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Oral presentation

  • Bioremediation of Acidified Pond and Soil
    SHIOMI Naofumi, NABESHIMA Risako, YASUDA Takako, IWASAKI Saori, YAMAGUCHI Yutaka, NAKAI Hiroaki, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Oral presentation

  • Effects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatus
    YOSHIMURA Satoshi, YEGANI Reza, MORIYA Kazunori, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Poster presentation

  • Detection of Single Nucleotide Polymorphysms by Affinity Chromatography with DNA Coupled Column
    NISHIWADA Yasushi, KAMURA Mitsumasa, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Poster presentation

  • Bioremediation of Herbicide-Polluted Soil with Self-Immobilized Microorganisms
    NAKAI Hiroaki, YAMAGUCHI Yutaka, YASUDA Takako, ARII Kenji, SAKAI Toyohide, KATSUDA Tomohisa, KATOH Shigeo, SHIOMI Naofumi
    平成17年度日本生物工学会大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conference
    Oral presentation

  • Rapid SNP Detection by Temperature Gradient Affinity Chromatography
    KATOH Shigeo, KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi
    25th International Symposium on the Separation of Proteins, Peptides & Polynucleotides, Nov. 2005, English, International Symposium on the Separation of Proteins, Peptides & Polynucleotides, St. Pete Beach, Florida, International conference
    Oral presentation

  • Effective Production of Astaxanthin from Haematococcus pluvialis by Use of Light Emitting Diodes
    SHIMAHARA Kazumichi, YAMAGAMI Keisuke, SHIRAISHI Hironori, KATSUDA Tomohisa, KATOH Shigeo
    関西地区3学協会合同大会, Nov. 2005, Japanese, 近畿化学協会, 化学工学会, 触媒学会, 大阪, Domestic conference
    Poster presentation

  • Detection of SNP Using Affinity Chromatography of Immobilized Oligonucleotide
    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo
    Young Asian Biochemical Engineers Community (YABEC) Symposium 2005, Oct. 2005, English, Young Asia Biochemical Engineers Community, Beijing, China, International conference
    Oral presentation

  • Effects of Operation Condition on Semi-Continuous Cultivation of Photosynthetic Bacterium Rhodobacter capsulatus
    KATSUDA Tomohisa, YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, INOUE Ryota, KATOH Shigeo
    化学工学会第37回秋季大会, Sep. 2005, Japanese, 化学工学会, 岡山, Domestic conference
    Poster presentation

  • Growth Sstability of Photosynthetic Bacteria : Effects of Supplemental Illumination and Ferrous Ion Concentration
    YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo
    The 12th European Congress on Biotechnology, Aug. 2005, English, European Federation of Biotechnology, Copenhagen, Denmark, International conference
    Poster presentation

  • Fed-Batch Cultivation of Haematococcus pluvialis under Illumination with LEDs for Production of Astaxanthin
    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo
    The 12th European Congress on Biotechnology, Aug. 2005, English, European Federation of Biotechnology, Copenhagen, Denmark, International conference
    Poster presentation

  • Temperature Gradient Elution of DNAs from an Oligo-DNA Immobilized Column
    KATSUDA Tomohisa, KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATOH Shigeo
    分離技術会年会2005, Jun. 2005, English, 分離技術会, 大阪, Domestic conference
    Poster presentation

  • Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis
    KATSUDA Tomohisa, LABABPOUR Abdolmajid, SHIMAHARA Kazumichi, KATOH Shigeo
    Asia-Pacific Biochemical Engineering Conference 2005, May 2005, English, The Korean Society for Biotechnology and Bioengineering, The Korean Institute of Chemical Engineers, Jeju, Korea, International conference
    Poster presentation

  • Detection of SNP using Affinity Chromatography of Immobilized Oligonucleotide
    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo
    The 4th Asia-Europe Biorecognition Engineering Society Conference, May 2005, English, Asia-Europe Biorecognition Engineering Society, Ansan, Korea, International conference
    Oral presentation

  • Effects of Nutrients Feeding Method on Astaxanthin Production by Haematococcus pluvialis
    KATSUDA Tomohisa, LABABPOUR Abdolmajid, KYOUI Yoshiaki, SHIMAHARA Kazumichi, KATOH Shigeo
    化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference
    Oral presentation

  • Elution Behavior of DNA from Oligo-DNA Immobilized Column under Temperature Gradient
    KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATSUDA Tomohisa, KATOH Shigeo
    化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference
    Oral presentation

  • Astaxanthin Production by H. pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps
    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo
    2004 AIChE Annual Meeting, Nov. 2004, English, American Institute of Chemical Engineers, Austin, TX, International conference
    Oral presentation

  • Development of Oligonucleotide Immobilized Capillary Chromatography
    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, HOSSAIN Zakir, KATOH Shigeo
    The 2nd International Conference on Bioseparation Engineering, Oct. 2004, English, International conference
    Oral presentation

  • フォトバイオリアクターにおける光強度分布と増殖速度安定性の工学的検討
    イエガニ レザ, 吉村 誠司, 森谷 一徳, 勝田 知尚, 加藤 滋雄
    日本生物工学会平成16年度大会, Sep. 2004, Japanese, 日本生物工学会, 名城大学, Domestic conference
    Oral presentation

  • オリゴDNA固定化担体を用いた一塩基多型(SNP)の検出法の検討
    西島 研, 嘉村 光真, 勝田 知尚, 加藤 滋雄
    日本生物工学会平成16年度大会, Sep. 2004, Japanese, 日本生物工学会, 名城大学, Domestic conference
    Oral presentation

  • Instability of Growth Rate in Dense Culture of Photosynthetic Cells
    YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, English, Young Asian Biochemical Engineers' Community (YABEC), Osaka, Japan, International conference
    Oral presentation

  • Induction of Astaxanthin Accumulation in H. pluvialis by IIIumination with LED Lamps and Fed Batch Addition of Nutrients
    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2004, Sep. 2004, English, Young Asian Biochemical Engineers' Community (YABEC), Osaka, Japan, International conference
    Oral presentation

  • Growth and Astaxanthin Accumulation in H. pluvialis Cultures Illuminated by LED Lamps
    Lababpour Abdolmajid, 羽田 圭司, 島原 一道, 勝田 知尚, 加藤 滋雄
    化学工学会第69年会, Apr. 2004, English, 化学工学会, 大阪府立大学, Domestic conference
    Oral presentation

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産におよぼす青色パルス光の影響
    嶋原 一道, LABABPOUR Abdolmajid, 羽田 圭志, 勝田 知尚, 加藤 滋雄
    第55回日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本大学, Domestic conference
    Oral presentation

  • 光合成微生物の半連続培養における増殖安定性におよぼす光強度分布の影響
    勝田 知尚, イエガニ レザ, 吉村 誠司, 藤井 信彰, 五十嵐 啓介, 加藤 滋雄
    第55回日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本大学, Domestic conference
    Oral presentation

  • パルス光照射下におけるヘマトコッカスによるアスタキサンチン生産
    勝田 知尚, LABABPOUR Abdolmajid, 嶋原 一道, 羽田 圭志, 加藤 滋雄
    化学工学会 第36回秋季大会, Sep. 2003, Japanese, 化学工学会, 東北大学, Domestic conference
    Oral presentation

  • The effects of light intensity distribution on the stability of growth rate of a photosynthetic microorganism Rhodobacter capsulatus
    YEGANI Reza, 藤井 信彰, 五十嵐 啓介, 吉村 誠司, 勝田 知尚, 加藤 滋雄
    化学工学会 第36回秋季大会, Sep. 2003, English, 化学工学会, 東北大学, Domestic conference
    Oral presentation

  • Cultivation of microalga in photobioreactors illuminated with LEDs
    KATOH Shigeo, LABABPOUR Abdolmajid, KATSUDA Tomohisa
    The 6th Italian Conference on Chemical and Process Engineering, Jun. 2003, English, 未記入, 未記入, International conference
    Oral presentation

  • Development of a new SNP detectable method by use of the immobilized oligonucleotide capillary chromatography
    Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Shigeo Katoh
    Asia-Europe Symposium on Biomolecular Recognition, May 2003, English
    Oral presentation

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産における光照射法の検討
    勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄
    化学工学会第68年会, Mar. 2003, Japanese
    Oral presentation

  • 二槽式バイオリアクターによる光合成微生物の生産性の向上
    藤井 信彰, 五十嵐 啓介, Reza Yegani, 勝田 知尚, 加藤 滋雄
    化学工学会第68年会, Mar. 2003, Japanese
    Oral presentation

  • Astaxanthin production by Haematococcus pluvialis using LED lamps
    Tomohisa Katsuda, Abdolmajid Lababpour, Kazumichi Shimahara, Shigeo Katoh
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2002, Nov. 2002, English
    Oral presentation

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産に及ぼす照射光の影響
    Abdolmajid Lababpour, 嶋原 一道, 勝田 知尚, 加藤 滋雄
    化学工学会第35回秋季大会, Sep. 2002, English
    Oral presentation

  • 発光ダイオードを用いた緑藻Haematococcus pluvialisによるアスタキサンチン生産
    勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄
    化学工学会第35回秋季大会, Sep. 2002, Japanese
    Oral presentation

  • タンパク質のリフォールディングにおよぼす変性剤除去の影響
    宇高 太郎, 神野 一也, M.D. Farshbaf, 勝田 知尚, 加藤 滋雄
    化学工学会第35回秋季大会, Sep. 2002, Japanese
    Oral presentation

  • 高効率土壌汚染浄化法を利用した酸性雨の中和
    安田 多賀子, 岩崎 沙織, 勝田 知尚, 塩見 尚史, 加藤 滋雄
    化学工学会鳥取大会, Jul. 2002, Japanese
    Oral presentation

  • 光合成微生物培養液中における光強度減衰モデルの適用性
    勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄
    化学工学会第67年会, Mar. 2002, Japanese
    Oral presentation

  • B. subtilisの自己固定化能力を利用した酸性雨の土壌中での中和
    安田 多賀子, 岩崎 沙織, 井上 洋子, 楠本 智子, 勝田 知尚, 塩見 尚史, 加藤 滋雄
    化学工学会第67年会, Mar. 2002, Japanese
    Oral presentation

  • Light attenuation in suspension of purple bacterium Rhodobacter capsulatus and green alga Chlamydomonas reinhardtii
    Tomohisa Katsuda, Nobuaki Fujii, Natsushi Takata, Shigeo Katoh
    Young Asian Biochemical Engineers' Community (YABEC) Symposium 2001, Oct. 2001, English
    Oral presentation

  • 光合成微生物培養液中における光強度減衰
    勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄
    日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 2001, Japanese
    Oral presentation

  • 光合成微生物培養液中での光強度減衰に及ぼす光源の影響
    勝田 知尚, 藤井 信彰, 加藤 滋雄
    化学工学会福井大会, Jul. 2001, Japanese
    Oral presentation

  • 光合成微生物培養液中での光強度減衰
    勝田 知尚, 藤井 信彰, 大嶋 寛, 加藤 滋雄
    化学工学会第66年会, Apr. 2001, Japanese
    Oral presentation

  • テーラー渦型反応器によるタンパク質の連続リフォールディング
    加藤 芳寛, M.D. Farshbaf, 宇高 太郎, 野田 秀夫, 勝田 知尚, 加藤 滋雄
    化学工学会第66年会, Apr. 2001, Japanese
    Oral presentation

  • 円筒型光リアクターの新規な光強度分布モデルと光合成細菌による水素生産への適用
    勝田 知尚, 有本 武司, 五十嵐 幸一, 東 雅之, 大嶋 寛
    化学工学会第32回秋季大会, Sep. 1999, Japanese
    Oral presentation

  • 円筒型フォトバイオリアクターの新規な光強度分布モデルと光合成微生物による水素生産への適用
    勝田 知尚, 大嶋 寛
    日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1999, Japanese
    Oral presentation

  • エタノールアミンを窒素源として用いた光合成細菌Rhodobacter capsulatusによる水素生産
    勝田 知尚, 大嶋 寛
    日本生物工学会光合成微生物研究部会夏期研究集会, Aug. 1998, Japanese
    Oral presentation

  • エタノールアミンを窒素源として用いた、Rhodobacter capsulatusによる水素生産
    勝田 知尚, 大嶋 寛, 東 雅之, 加藤 錠治, 高桑 進
    平成9年度日本生物工学会大会, Sep. 1997, Japanese
    Oral presentation

  • Hydrogen detectable color indicator for screening of microorganisms
    Tomohisa Katsuda, Hiroshi Ooshima, Masayuki Azuma, Jyoji Kato
    化学工学会第29回秋季大会, Sep. 1996, English
    Poster presentation

■ Affiliated Academic Society
  • European Federation of Biotechnology
    May 2007 - Present

  • Asian Federation of Biotechnology

  • 日本生物工学会

  • 化学工学会

■ Research Themes
  • 培養温度制御に基づく真核細胞への遺伝子導入の効率化
    勝田 知尚
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2021 - 31 Mar. 2024
    本研究では,研究代表者らが緑藻の培養で見出した,培養温度を適切な範囲で周期的に変化させると培養液中の細胞間で細胞周期が同調するという知見に基づき,真核細胞の細胞周期を同調させることにより,核膜の消失から再構成の間にあわせて外来遺伝子を導入できるようにし,これによって外来遺伝子の核内移行,ならびに核DNAとの接触を促進させて,真核細胞への遺伝子導入の効率化を図ることを検討する. 令和3年度には,開放型の有糸分裂を行う昆虫細胞に注目し,培養温度をプログラム制御できるインキュベーターを用いて培養を行い,細胞周期が同調する温度制御条件の検討を行った.昆虫細胞には,組換えタンパク質の発現に広く利用されており,子宮頸がんワクチンの生産にも利用されているTrichoplusia ni BTI-TN-5B1-4 (High Five)細胞を用いた. はじめに,培養温度を最適よりも高温側で一定に保持して増殖挙動を調べた.High Five細胞は35℃までは最適温度である27℃のときと同等の増殖速度ならびに最高到達細胞密度を示した.しかし,37℃では増殖が顕著に抑制され,39℃では増殖しないことが分かった.培養液中のそれぞれの細胞の細胞周期をフローサイトメーターにより分析したところ,G2/M期にある細胞の全細胞数に対する割合が27℃の15-20%から37℃の30-40%まで,温度上昇に伴って増加することを見出した. そこで,培養温度は27℃と37℃を12 h周期で変化させることとし,High Five細胞の細胞周期の同調を試みた.しかし,植継ぎを行いながら6 dにわたって培養を継続したが,細胞周期が同調したときに見られる細胞密度の倍加は観察されなかった.High Five細胞では緑藻と同様にして細胞周期を同調させることは困難であることが分かった.

  • 勝田 知尚
    学術研究助成基金助成金/基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigator
    Competitive research funding

  • Production of influenza virus-like particles in recombinant insect cells
    Yamaji Hideki, KATSUDA Tomohisa
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2015 - 31 Mar. 2019
    Production of influenza virus-like particles in recombinant insect cells was investigated. The cDNA fragments encoding hemagglutinin (HA) and matrix protein 1 (M1) of an influenza virus A were separately cloned into the plasmid vector pIHAbla and pIHAneo. The pIHAbla and pIHAneo contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with either a blasticidin or neomycin resistance gene for use as a selectable marker, respectively. After cotransfection with the prepared plasmids, High Five cells were incubated with blasticidin and G418, and cells resistant to the antibiotics were effectively obtained. Sucrose density-gradient sedimentation analysis of the culture supernatant showed that secreted HA and M1 molecules were produced in a particulate form.

  • SUZUKI Hiroshi, KOMODA Yoshiyuki, KATSUDA Tomohisa
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2008 - 2010
    In order to investigate the formation mechanism of liposomes in a micro-channel, an experimental study has been performed. The Lagrangian observations and velocity field measurements by a micro-PTV were carried out by use of a movable stage. From the results, it was found that the lipid film pre-coated on the wall were taken into the liquid/air interface and the rod-like liposomes were formed between the interface and the wall and that the rod-like liposomes becomes spherical liposomes in a flow. It was also found that the rod-like liposomes serially induced from the interface by the strong reverse flow existing near the interface
    Competitive research funding

  • 産業技術研究「活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発」
    勝田 知尚
    2008, Principal investigator
    Competitive research funding

  • 昇温溶出アフィニティークロマトグラフィーによる一塩基多型の検出
    勝田 知尚
    2007, Principal investigator
    Competitive research funding

  • 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発
    勝田 知尚
    2006, Principal investigator
    Competitive research funding

  • 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発
    勝田 知尚
    2005, Principal investigator
    Competitive research funding

  • Integrate System for Creation, Detection and Production of Antibodies with Variety of Characteristics
    KATOH Shigeo, KATUSDA Tomohisa, SHIOMI Naofumi
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2003 - 2004
    In the fields of development of pharmaceuticals, medical diagnosis-analysis and bioprocesses, production of variety of antibodies, detection of the interactions between antibody and protein, screening methods of antibodies and effective production processes of antibodies are essential. In this work, we developed the simple and rapid methods to obtain antibody ligands for recognition and detection of target proteins, based on our recent researches on production of large libraries of antibodies, a high sensitivity detection method of proteins and effective production of antibodies by secretion from yeast cells. 1.A colony lift assay for detection of antibody secreted from E. coli was developed by use of antigen-adsorbed PVDF membrane and liposome immunoblotting assay. By use of this method, high density and micro-assay was possible and the number of samples treated was increased. 2.By use of HRP encapsulating immunoliposomes and hydrophobic membranes, an immunoblotting assay method with two orders of magnitude higher sensitivity, compared with the conventional immunoblotting assay using HRP-conjugated antibody, was developed. 3.A large library of whole antibodies was obtained based on mating of yeast cells transformed by libraries of H- and L-chains, and aproduction method of antibodies by secretion from yeast cells was established. 4.An affinity biopanning method utilizing immunoliposomes was developed and it was applied to biopanning selection with a high efficiency.

■ Industrial Property Rights
  • リポソームの製造方法
    SUZUKI HIROSHI, KOMODA YOSHIYUKI, KATSUDA TOMOHISA
    特願2012-553652, 06 Jan. 2012, 大学長, 特許5904555, 25 Mar. 2016
    Patent right

  • リポソーム製剤の製造方法
    SUZUKI HIROSHI, KATSUDA TOMOHISA, KATOH SHIGEO, KOMODA YOSHIYUKI, USUI HIROMOTO
    特願2007-134414, 21 May 2007, 大学長, 特許5126874, 09 Nov. 2012
    Patent right

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