研究者紹介システム

深山 浩
フカヤマ ヒロシ
大学院農学研究科 資源生命科学専攻
准教授
農学関係
Last Updated :2021/04/12

研究者情報

所属

  • 【主配置】

    大学院農学研究科 資源生命科学専攻
  • 【配置】

    農学部 資源生命科学科

学位

  • 博士(学術), 神戸大学

授業科目

ジャンル

  • 環境・交通 / 農業

コメントテーマ

  • 熱帯有用植物学
  • 南米野生イネ
  • 浮稲
  • 冠水耐性稲
  • イネの光合成の改良

研究活動

研究分野

  • 環境・農学 / 作物生産科学

受賞

  • 2019年03月 日本作物学会, 日本作物学会賞, イネの光合成能力の改良に向けた炭素同化関連タンパク質の生理・生化学的研究

    深山 浩

    国内学会・会議・シンポジウム等の賞

  • 2015年03月 神戸大学農学部六篠会, 六篠論文賞, CO2-responsive CONSTANS, CONSTANS-like, and time of chlorophyll a/b binding protein Expression1 protein is a positive regulator of starch synthesis in vegetative organs of rice.

    森田 隆太郎, 杉野 充保, 畠中 知子, 三十尾 修司, 深山 浩

    国際的に評価の高い学術雑誌に論文が掲載されたため.

    出版社・新聞社・財団等の賞

  • 2014年09月 日本作物学会, 日本作物学会優秀発表賞, 耐塩性インディカイネ交雑由来倍加半数体系統の継続的耐塩性評価

    板倉 俊介, 深山 浩, 畠中 知子, 三十尾 修司

    日本国

    国内学会・会議・シンポジウム等の賞

論文

  • CO2 Responsive CCT Protein Stimulates the Ectopic Expression of Particular Starch Biosynthesis-Related Enzymes, which Markedly Change the Structure of Starch in the Leaf Sheaths of Rice.

    森田 隆太郎, クロフツ 尚子, 柴谷 直樹, 三浦 聡子, 保坂 優子, 追留 那緒子, 池田 健一, 藤田 直子, 深山 浩

    2019年05月, Plant and Cell Physiology, 60, 961 - 972, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hajime Tomimatsu, Tsuyoshi Sakata, Hiroshi Fukayama, Yanhong Tang

    Long-term high CO2 exposure accelerates photosynthetic induction response due to rapid light increase. However, it is unclear whether the acceleration is caused by acclimation of photosynthetic components (long-term CO2 effect) and/or by the sufficient substrate under high CO2 at the measurement (short-term CO2 effect). Populus koreana x trichocarpa cv. Peace has wide-open stomata almost not responding to changes of photon flux density. Using this species, we examined the long- and short-term CO2 effects on photosynthetic induction by focusing on biochemical components. We grew the plants under [CO2] of 380, 700 and 1020 mol CO2 mol(-1) air and measured the photosynthetic induction response under [CO2] of 380 and 1020 mol CO2 mol(-1) air. Despite significant reduction in Rubisco content and light-saturated photosynthetic rate in the leaves from the high growth CO2, the photosynthetic induction time was similar in leaves from different growth CO2 plants when measurement [CO2] was the same. The induction, however, was significantly fast at the higher than at the lower measurement [CO2], regardless of growth CO2 of the plants. These results demonstrate that the acceleration of apparent photosynthetic induction under high CO2 environment was mainly contributed by a short-term CO2 effect rather than by a long-term acclimation effect when stomatal limitation is not the major factor.

    OXFORD UNIV PRESS, 2019年03月, Tree Physiology, 39 (3), 474 - 483, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga SHIMAKAWA, Kentaro IFUKU, Yuji SUZUKI, Amane MAKINO, Kimitsune ISHIZAKI, Hiroshi FUKAYAMA, Ryutaro MORITA, Katsuhiko SAKAMOTO, Akiko NISHI, Chikahiro MIYAKE

    Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2018年12月, Bioscience, Biotechnology and Biochemistry, 82 (12), 2072 - 2083, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Internodal elongation under submergence in the Amazonian wild rice species Oryza glumaepatula: the growth response is induced by hypoxia but not by ethylene

    Daisuke Sasayama, Takuma Okishio, Tatsuya Hirano, Hiroshi Fukayama, Tomoko Hatanaka, Masahiro Akimoto, Tetsushi Azuma

    2018年02月, Plant Growth Regulation, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rubisco small subunits of C4 plants, Napier grass and guinea grass confer C4-like catalytic properties on Rubisco in rice.

    深山 浩, 小原 崇, 塩見 慶太, 森田 隆太郎, 笹山 大輔, 畠中 知子, 東 哲司

    2018年, Plant Production Science, DOI: 10.1080/1343943X.2018.154, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice.

    Hiroshi Fukayama, Akina Mizumoto, Chiaki Ueguchi, Jun Katsunuma, Ryutaro Morita, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma

    2018年, Photosynthesis Research, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Activation State of Rubisco Decreases with the Nitrogen accumulation during the Reproductive Stage in Soybean [Glycine max (L.) Merr.]

    Kazuma Sakoda, Seita Suzuki, Hiroshi Fukayama, Yu Tanaka, Tatsuhiko Shiraiwa

    2018年, Photosynthetica, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryutaro Morita, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Hiroshi Fukayama

    © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Production of bioethanol from rice straw has attracted attention from the point of effective use of agricultural residue. Starch content is an important determinant for bioethanol production from rice straw. The overexpression of CO2-responsive CCT protein (CRCT), which is the positive regulator of starch synthesis in vegetative organs, notably increased the starch content in rice straw. To produce the bioethanol from rice straw, the dilute acid pretreatment is a general pretreatment method. Importantly, the glucose yields in liquid hydrolyzate after dilute acid pretreatment was markedly increased in the CRCT overexpression lines compared with non-transgenic rice. In addition, the overexpression of CRCT enhanced the biomass production. In contrast, CRCT did not affect on the glucose yields from cellulose in acid-insoluble residue obtained after dilute acid pretreatment. With respect to byproduct in liquid hydrolyzate which inhibits the fermentation, the formic acid content was increased, whereas the furfural, 5-hydroxymethylfurfural and acetic acid contents were unchanged by the overexpression of CRCT. These results demonstrate that genetic engineering of CRCT is an effective method to increase the bioethanol production from rice straw.

    日本作物学会, 2017年10月02日, Plant Production Science, 20 (4), 441 - 447, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Sasayama, Kenta Ikemachi, Hisateru Sakai, Hiroshi Fukayama, Kazuyuki Itoh, Tetsushi Azuma

    The semiaquatic plants, Sagittaria trifolia and Sagittaria pygmaea, are perennial and troublesome weeds of rice paddy fields. These species mainly reproduce vegetatively via tubers, which sprout after the rice paddy fields are irrigated. Sprouting finally leads to the emergence of the shoots from the water in the paddy fields. In both species, the first internode elongates around the time of emergence and this lifts the shoot meristems in the soil, aiding in the establishment of the young plants. Therefore, elongation of the internode is a factor that determines the weed damage to rice caused by these species. In this study, the first internode elongation of both species was characterized. During emergence, S. trifolia and S. pygmaea showed distinct growth patterns. In S. trifolia, the internodes elongated before the emergence of the shoots from the water; whereas, in S. pygmaea, the internodes started to elongate only after emergence. We examined environmental prerequisites for internode elongation, and found that the internode elongation of S. trifolia was induced by submergence and was independent of the soil cover, while that of S. pygmaea required both submergence and covering. Next, we determined which gaseous factors were responsible for internode elongation. Treatment with ethylene, which enhances growth of several other aquatic and semiaquatic plants, did not stimulate internode elongation in either species under an anaerobic condition. Our results suggested that the gaseous factor stimulating internode elongation in S. trifolia and S. pygmaea may be oxygen depletion and carbon dioxide, respectively.

    WILEY-BLACKWELL, 2016年12月, WEED BIOLOGY AND MANAGEMENT, 16 (4), 147 - 156, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koichi Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    Rubisco is the key enzyme of photosynthetic CO2 fixation, whereas a Rubisco small subunit homolog (OsRbcS1) in rice, was exclusively expressed in non-photosynthetic organs. In this study, OsRbcS1-like genes were identified in several plant species including foxtail millet (SiRbcS1), tomato (SlRbcS1), Lotus japonicus (LjRbcS1), grape (VvRbcS1A, VvRbcS1B) and Selaginella moellendorffii (SmRbcS1A, SmRbcS1B). RT-PCR analysis revealed that SiRbcS1 was expressed in seeds of foxtail millet. In tomato, SlRbcS1 was expressed in the stamen, pistil and green fruit. In L. japonicus, LjRbcS1 was expressed in the root, nodule and seed. In grape, both VvRbcS1A and VvRbcS1B were expressed in mature leaves and the green berry. In S. moellendorffii, SmRbcS1A and SmRbcS1B displayed similar expression patterns and were expressed in the rhizome and root. Although these OsRbcS1-like genes were expressed in photosynthetic organs in S. moellendorffii, its expression levels were higher in non-photosynthetic organs than in photosynthetic organs. These results suggest that OsRbcS1-like RbcS functions in metabolic pathways other than photosynthetic CO2 fixation in various organs.

    Elsevier, 2016年12月01日, Plant Gene, 8, 26 - 31, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryutaro Morita, Kanako Inoue, Ken-ichi Ikeda, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    CO2-responsive CCT protein (CRCT) is the suggested positive regulator of starch synthesis in vegetative organs, particularly the leaf sheath of rice. In this study, we analyzed the effects of the starch level in the leaf sheath on the photosynthetic rate in the leaf blade using CRCT overexpression and RNA interference (RNAi) knockdown transgenic rice grown under ambient (38 Pa) or elevated (100 Pa) CO2 conditions. In leaf sheath, the starch content was markedly changed in relation to CRCT expression levels under both CO2 conditions. In contrast, the soluble sugar and starch contents of the leaf blade were markedly increased in the knockdown line grown under elevated CO2 conditions. The overexpression or RNAi knockdown of CRCT did not cause large effects on the photosynthetic rate of the transgenic lines grown under ambient CO2 condition. However, the photosynthetic rate of the overexpression line was enhanced, while that of the knockdown line was substantially decreased under elevated CO2 conditions. These photosynthetic rates were weakly correlated with the nitrogen contents and negatively correlated with the total nonstructural carbohydrate contents. Thus, the capacity for starch synthesis in leaf sheath, which is controlled by CRCT, can indirectly affect the carbohydrate content, and then the photosynthetic rate in the leaf blade of rice grown under elevated CO2 conditions.

    OXFORD UNIV PRESS, 2016年11月, PLANT AND CELL PHYSIOLOGY, 57 (11), 2334 - 2341, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takehiro Sejima, Hitomi Hanawa, Ginga Shimakawa, Daisuke Takagi, Yuji Suzuki, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

    This study aims to elucidate the molecular mechanism for the transient increase in the O-2-uptake rate in tobacco (Nicotiana tabacum cv Xanthi) leaves after turning off actinic lights (ALs). The photosynthetic O-2 evolution rate reaches a maximum shortly after the onset of illumination with ALs and then decreases to zero in atmospheric CO2/O-2 conditions. After turning off the ALs, tobacco leaves show a transient increase in the O-2-uptake rate, the post-illumination transient O-2-uptake, and thereafter, the O-2-uptake rate decreases to the level of the dark-respiration rate. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], maintained a steady-state value distinct from the photosynthetic O-2-evolution rate. In high-[CO2] conditions, the photosynthetic O-2-evolution rate and Y(II) showed a parallel behavior, and the post-illumination transient O-2-uptake was suppressed. On the other hand, in maize leaves (a C4 plant), even in atmospheric CO2/O-2 conditions, Y(II) paralleled the photosynthetic O-2-evolution rate and the post-illumination transient O-2-uptake was suppressed. Hypothesizing that the post-illumination transient O-2-uptake is driven by C3 plant photorespiration in tobacco leaves, we calculated both the ribulose 1,5-bisphosphate carboxylase-and oxygenase-rates (Vc and Vo) from photosynthetic O-2-evolution and the post-illumination transient O-2-uptake rates. These values corresponded to those estimated from simultaneous chlorophyll fluorescence/O-2-exchange analysis. Furthermore, the H+-consumption rate for ATP synthesis in both photosynthesis and photorespiration, calculated from both Vc and Vo that were estimated from chlorophyll fluorescence/CO2-exchange analysis, showed a positive linear relationship with the dissipation rate of the electrochromic shift signal. Thus, these findings support our hypothesis.

    WILEY-BLACKWELL, 2016年02月, PHYSIOLOGIA PLANTARUM, 156 (2), 227 - 238, 英語

    [査読有り]

    研究論文(学術雑誌)

  • ダイズ体細胞胚長期懸濁培養におけるマルトースの効果に関する一考察

    畠中 知子, 三十尾 修司, 深山 浩, 木原 由貴, 左海 庸光, 瀬田 聡美

    ダイズの組織培養による再分化・植物体再生は,未熟子葉から体細胞胚を誘導し,増殖・形態分化・成熟の各ステージを経て,植物体を再生させる方法が主流である.このプロトコールはモデル品種であるJackでほぼ確立されているが,最適な培養方法は現在でも遺伝子型の違いに大きく左右される.代表的な日本産黒ダイズである丹波黒ではまだ正式な成功例が報告されていないが,我々の研究室では,Jackのプロトコールを改変することにより,丹波黒ダイズから体細胞胚を誘導・増殖し,効率は低いものの植物体再生にも成功してきた.これらの実験過程で,成熟培養だけでなく増殖培養でも培地に加える糖をスクロースからマルトースに置き換えることで,明らかな再分化促進効果を表すことがわかってきた.通常,増殖培養を2年以上も続けると,モデル品種であるJackでもその後の体細胞胚成熟効率が下がり,次第に再

    2015年11月, 作物研究, 60, 47 - 53, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Fukayama, Chisato Masumoto, Yojiro Taniguchi, Akiko Baba-Kasai, Yuuki Katoh, Hiroshi Ohkawa, Mitsue Miyao

    To verify the presence of enolase related to the chloroplastic glycolysis in rice, database search was carried out and identified seven putative enolase genes in the rice genome. Among them, OsEno1 and OsEno3 encode long proteins with N-terminal extensions. GFP protein fusions of these N-terminal extensions were both targeted to plastids of onion epidermal cell. Promoter::GUS analysis showed that OsEno3 was highly expressed in young developing leaves, but its expression was drastically decreased during leaf development and greening. On the other hand, the expression of OsEno1 was low and detected in limited portions such as leaf sheath at the tiller base. Recombinant OsEno1 protein showed enolase activity with a pH optimum at pH 8.0, whereas OsEno3 did not exhibit detectable activity. Although it remains obscure if OsEno3 encodes a functional enolase in vivo, our results demonstrate that the entire glycolytic pathway does not operate in rice chloroplasts.

    TAYLOR & FRANCIS LTD, 2015年03月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79 (3), 402 - 409, 英語

    [査読有り]

    研究論文(学術雑誌)

  • CO2-responsive CONSTANS, CONSTANS-like, and time of chlorophyll ab binding protein Expression1 protein is a positive regulator of starch synthesis in vegetative organs of rice.

    Ryutaro Morita, Miho Sugino, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    2015年02月, Plant Physiology, 167 (4), 1321 - 1331, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Fukayama, Atsushi Koga, Tomoko Hatanaka, Shuji Misoo

    Effects of overexpression of high activity-type Rubisco small subunit (RbcS) from a cold-resistant plant, timothy (Phleum pratense), on kinetic properties of Rubisco were studied in rice (Oryza sativa). The full-length mRNA sequence of timothy RbcS (PpRbcS1) was determined by 5'RACE and 3'RACE. The coding sequence of PpRbcS1 was fused to the chlorophyll a/b-binding protein promoter and introduced into rice. PpRbcS was highly expressed in leaf blade and accounted for approximately 30 % of total RbcS in homozygous transgenic lines. However, the catalytic turnover rate and K m for CO2 of Rubisco did not significantly change in these transgenic lines compared to non-transgenic rice, suggesting that PpRbcS1 is not effective for improvement of catalytic efficiency of rice Rubisco. The photosynthetic rate and growth were essentially unchanged, whereas the photosynthetic rate at low CO2 condition was marginally increased in transgenic lines. Rubisco content was significantly increased, whereas soluble protein, nitrogen, and chlorophyll contents were unchanged in transgenic lines compared to non-transgenic rice. Because the kinetic properties were similar, observed slight increase in photosynthetic rate at low CO2 is considered to be large due to increase in Rubisco content in transgenic lines. Introduction of foreign RbcS is an effective approach for the improvement of Rubisco kinetics and photosynthesis. However, in this study, it was suggested that RbcS of high activity-type Rubisco, even showing higher amino acid identity with rice RbcS, did not always enhance the catalytic turnover rate of Rubisco in rice. Thus, we should carefully select RbcS to be overexpressed before introduction.

    Kluwer Academic Publishers, 2015年, Photosynthesis Research, 124 (1), 57 - 65, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takehiro Sejima, Daisuke Takagi, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

    Under field conditions, the leaves of plants are exposed to fluctuating light, as observed in sunfleck. The duration and frequency of sunfleck, which is caused by the canopy being blown by the wind, are in the ranges from 0.2 to 50 s, and from 0.004 to 1 Hz, respectively. Furthermore, > 60% of the sunfleck duration ranges from 0.2 to 0.8 s. In the present research, we analyzed the effects of repetitive illumination by short-pulse (SP) light of sunflower leaves on the photosynthetic electron flow. The duration of SP light was set in the range from 10 to 300 ms. We found that repetitive illumination with SP light did not induce the oxidation of P700 in PSI, and mainly inactivated PSI. Increases in the intensity, duration and frequency of SP light enhanced PSI photoinhibition. PSI photoinhibition required the presence of O-2. The inactivation of PSI suppressed the net CO2 assimilation. On the other hand, the increase in the oxidized state of P700 suppressed PSI inactivation. That is, PSI with a reduced reaction center would produce reactive oxygen species (ROS) by SP light, leading to PSI photodamage. This mechanism probably explains the PSI photodamage induced by constant light.

    OXFORD UNIV PRESS, 2014年06月, PLANT AND CELL PHYSIOLOGY, 55 (6), 1184 - 1193, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Fukayama, Naoko Fujiwara, Tomoko Hatanaka, Shuji Misoo, Mitsue Miyao

    Phosphoenolpyruvate carboxylase (PEPC) undergoes activity regulation through reversible phosphorylation. The day/night phosphorylation of leaf PEPC in 27 C-3 plant species was analyzed by immunoblotting. PEPC was phosphorylated in the daytime in 12 species, whereas it was phosphorylated at night in three species, rice, Monochoria vaginalis, and Sagittaria trifolia, all of which are hygrophytic monocots. Immunoblot analysis of isolated chloroplasts of M. vaginalis identified a PEPC protein inside the chloroplast in addition to cytosolic isozyme(s) as previously shown in genus Oryza. Using transgenic rice overexpressing the maize PEPC in the cytosol, we confirmed that the cytosolic PEPC underwent the nocturnal phosphorylation. These results suggest the interrelationship between the presence of chloroplastic PEPC and the nocturnal phosphorylation of cytosolic isozyme(s).

    TAYLOR & FRANCIS LTD, 2014年04月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 78 (4), 609 - 613, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A Dominant Mutation in LOV2 Domain Vicinity Impairs Phototropin1 Signaling in Tomato

    Sulabha Sharma, Eros Kharshiing, Ankanagari Srinivas, Kazunori Zikihara, Satoru Tokutomi, Akira Nagatani, FUKAYAMA HIROSHI, Reddaiah Bodanapu, Rajendra K Behera, Yellamaraju Sreelakshmi, Rameshwar Sharma

    2014年04月, Plant Physiology, 164, 2030 - 2044, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nocturnal phosphorylation of phosphoenolpyruvate carboxylase in the leaves of hygrophytic C3 monocots.

    FUKAYAMA HIROSHI, Naoko Fujiwara, Tomoko Hatanaka, Shuji Misoo, Mitsue Miyao

    Phosphoenolpyruvate carboxylase (PEPC) undergoes activity regulation through reversible phosphorylation. The day/night phosphorylation of leaf PEPC in 27 C3 plant species was analyzed by immunoblotting. PEPC was phosphorylated in the daytime in 12 species, whereas it was phosphorylated at night in three species, rice, Monochoria vaginalis and Sagittaria trifolia, all of which a

    2014年02月, Bioscience, Biotechnology, and Biochemistry, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Effect of free-air CO2 enrichment (FACE) and soil/water warming on leaf photosynthetic parameters in rice

    Adachi Minako, Hasegawa Toshihiro, Fukayama Hiroshi, Tokida Takeshi, Sakai Hidemitsu, Matsunami Toshinori, Nakamura Hirofumi, Sameshima Ryoji, Okada Masumi

    2014年02月, Plant Cell physiology, 55, 370–380, 英語

    [査読有り][招待有り]

    研究論文(学術雑誌)

  • Unusual small subunit, OsRbcS1 that is not expressed in photosynthetic tissues alters the catalytic properties of Rubisco in rice

    Morita K, Hatanaka T, Misoo S, Fukayama H

    American Society of Plant Biologists, 2014年, Plant Physiology, 164 (1), 69 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koichi Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    Rubisco small subunits (RbcSs) are encoded by a nuclear multigene family in plants. Five RbcS genes, OsRbcS1, OsRbcS2, OsRbcS3, OsRbcS4, and OsRbcS5, have been identified in rice (Oryza sativa). Among them, the amino acid sequence of OsRbcS1 differs notably from those of other rice RbcSs. Phylogenetic analysis showed that OsRbcS1 is genetically distant from other rice RbcS genes and more closely related to RbcS from a fern and two woody plants. Reverse transcription-PCR and promoter beta-glucuronidase analyses revealed that OsRbcS1 was not expressed in leaf blade, a major photosynthetic organ in rice, but was expressed in leaf sheath, culm, anther, and root central cylinder. In leaf blade of transgenic rice overexpressing OsRbcS1 and leaf sheath of nontransgenic rice, OsRbcS1 was incorporated into the Rubisco holoenzyme. Incorporation of OsRbcS1 into Rubisco increased the catalytic turnover rate and K-m for CO2 of the enzyme and slightly decreased the specificity for CO2, indicating that the catalytic properties were shifted to those of a high-activity type Rubisco. The CO2 assimilation rate at low CO2 partial pressure was decreased in overexpression lines but was not changed under ambient and high CO2 partial pressure compared with nontransgenic rice. Although the Rubisco content was increased, Rubisco activation state was decreased in overexpression lines. These results indicate that the catalytic properties of Rubisco can be altered by ectopic expression of OsRbcS1, with substantial effects on photosynthetic performance in rice. We believe this is the first demonstration of organ-specific expression of individual members of the RbcS gene family resulting in marked effects on Rubisco catalytic activity.

    AMER SOC PLANT BIOLOGISTS, 2014年01月, PLANT PHYSIOLOGY, 164 (1), 69 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Wataru Yamori, Chisato Masumoto, Hiroshi Fukayama, Amane Makino

    The role of Rubisco activase in steady-state and non-steady-state photosynthesis was analyzed in wild-type (Oryza sativa) and transgenic rice that expressed different amounts of Rubisco activase. Below 25 degrees C, the Rubisco activation state and steady-state photosynthesis were only affected when Rubisco activase was reduced by more than 70%. However, at 40 degrees C, smaller reductions in Rubisco activase content were linked to a reduced Rubisco activation state and steady-state photosynthesis. As a result, overexpression of maize Rubisco activase in rice did not lead to an increase of the Rubisco activation state, nor to an increase in photosynthetic rate below 25 degrees C, but had a small stimulatory effect at 40 degrees C. On the other hand, the rate at which photosynthesis approached the steady state following an increase in light intensity was rapid in Rubisco activase-overexpressing plants, intermediate in the wild-type, and slowest in antisense plants at any leaf temperature. In Rubisco activase-overexpressing plants, Rubisco activation state at low light was maintained at higher levels than in the wild-type. Thus, rapid regulation by Rubisco activase following an increase in light intensity and/or maintenance of a high Rubisco activation state at low light would result in a rapid increase in Rubisco activation state and photosynthetic rate following an increase in light intensity. It is concluded that Rubisco activase plays an important role in the regulation of non-steady-state photosynthesis at any leaf temperature and, to a lesser extent, of steady-state photosynthesis at high temperature.

    WILEY-BLACKWELL, 2012年09月, PLANT JOURNAL, 71 (6), 871 - 880, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

    Chisato Masumoto, Hiroshi Fukayama, Tomoko Hatanaka, Naotsugu Uchida

    The activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which catalyses CO2 fixation in photosynthesis, requires the assistance of the regulatory protein Rubisco activase. Rubisco activase promotes carbamylation of Rubisco by releasing inhibitory sugar phosphates bound to the catalytic site of Rubisco in the light. To clarify the effects of Rubisco activase contents on the photosynthesis of rice, we investigated the steady-state photosynthesis and light-induction of photosynthesis in transgenic rice plants, in which leaf Rubisco activase levels were reduced. The reduction in Rubisco activase did not affect steady-state photosynthesis under high light intensity until the Rubisco activase was about 15% of that in control plants. However, light-induction of photosynthesis, namely, increase in photosynthetic rate following a transition from a low to high light intensity, was considerably low in transgenic rice plants with 20-25% Rubisco activase, which was sufficient to support the steady-state photosynthesis. In addition, the Rubisco activase content was highly correlated with the initial rate of Rubisco activation after the increase in light intensity. These results suggest that Rubisco activase in rice leaves largely limits the light-induction of photosynthesis, but not steady-state photosynthesis.

    CROP SCIENCE SOC JAPAN, 2012年07月, PLANT PRODUCTION SCIENCE, 15 (3), 174 - 182, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

    Chisato Masumoto, Hiroshi Fukayama, Tomoko Hatanaka, Naotsugu Uchida

    The activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which catalyses CO2 fixation in photosynthesis, requires the assistance of the regulatory protein Rubisco activase. Rubisco activase promotes carbamylation of Rubisco by releasing inhibitory sugar phosphates bound to the catalytic site of Rubisco in the light. To clarify the effects of Rubisco activase contents on the photosynthesis of rice, we investigated the steady-state photosynthesis and light-induction of photosynthesis in transgenic rice plants, in which leaf Rubisco activase levels were reduced. The reduction in Rubisco activase did not affect steady-state photosynthesis under high light intensity until the Rubisco activase was about 15% of that in control plants. However, light-induction of photosynthesis, namely, increase in photosynthetic rate following a transition from a low to high light intensity, was considerably low in transgenic rice plants with 20-25% Rubisco activase, which was sufficient to support the steady-state photosynthesis. In addition, the Rubisco activase content was highly correlated with the initial rate of Rubisco activation after the increase in light intensity. These results suggest that Rubisco activase in rice leaves largely limits the light-induction of photosynthesis, but not steady-state photosynthesis.

    CROP SCIENCE SOC JAPAN, 2012年07月, PLANT PRODUCTION SCIENCE, 15 (3), 174 - 182, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Fukayama, Chiaki Ueguchi, Kaoru Nishikawa, Nobuaki Katoh, Chie Ishikawa, Chisato Masumoto, Tomoko Hatanaka, Shuji Misoo

    The effects of overexpression of Rubisco activase on photosynthesis were studied in transgenic rice expressing barley or maize Rubisco activase. Immunoblot and SDS-PAGE analyses showed that transgenic lines from both gene constructs expressed the foreign Rubisco activase at high levels. The activation state of Rubisco in transgenic lines was slightly higher than that in non-transgenic plants (NT). In addition, light activation of Rubisco was significantly more rapid in transgenic lines compared with NT. These findings indicate that the overexpression of Rubisco activase can enhance Rubisco activation. However, despite enhanced activation of Rubisco in these transgenic plants, the CO2 assimilation rate at ambient CO2 conditions was decreased. This decrease in CO2 assimilation rate was observed in both young developing and mature leaves independent of nitrogen nutrition. The contents of nitrogen and Chl did not differ significantly between transformants and NT; however, Rubisco content was substantially decreased in transgenic lines. There was no evidence for reduced transcription of RbcS or RbcL in these transgenic lines; in fact, transcript levels were marginally increased compared with NT. These results indicate that the overexpression of Rubisco activase leads to a decrease in Rubisco content, possibly due to post-transcriptional mechanisms.

    OXFORD UNIV PRESS, 2012年06月, PLANT AND CELL PHYSIOLOGY, 53 (6), 976 - 986, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Fukayama, Chiaki Ueguchi, Kaoru Nishikawa, Nobuaki Katoh, Chie Ishikawa, Chisato Masumoto, Tomoko Hatanaka, Shuji Misoo

    The effects of overexpression of Rubisco activase on photosynthesis were studied in transgenic rice expressing barley or maize Rubisco activase. Immunoblot and SDS-PAGE analyses showed that transgenic lines from both gene constructs expressed the foreign Rubisco activase at high levels. The activation state of Rubisco in transgenic lines was slightly higher than that in non-transgenic plants (NT). In addition, light activation of Rubisco was significantly more rapid in transgenic lines compared with NT. These findings indicate that the overexpression of Rubisco activase can enhance Rubisco activation. However, despite enhanced activation of Rubisco in these transgenic plants, the CO2 assimilation rate at ambient CO2 conditions was decreased. This decrease in CO2 assimilation rate was observed in both young developing and mature leaves independent of nitrogen nutrition. The contents of nitrogen and Chl did not differ significantly between transformants and NT; however, Rubisco content was substantially decreased in transgenic lines. There was no evidence for reduced transcription of RbcS or RbcL in these transgenic lines; in fact, transcript levels were marginally increased compared with NT. These results indicate that the overexpression of Rubisco activase leads to a decrease in Rubisco content, possibly due to post-transcriptional mechanisms.

    OXFORD UNIV PRESS, 2012年06月, PLANT AND CELL PHYSIOLOGY, 53 (6), 976 - 986, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Identification of Two Plastid-Targeted beta-Amylases in Rice

    Tatsuya Hirano, Yusuke Takahashi, Hiroshi Fukayama, Hiroyasu Michiyama

    In order to identify beta-amylase isoforms acting inside plastids in rice (Oryza sativa L.), in the rice genome database we searched the genes predicted to encode beta-amylase-like proteins, and designated them OsBAM1-9. OsBAM1, OsBAM7, and OsBAM9 genes were mainly expressed in germinating seeds and developing caryopses. In contrast, the transcripts of OsBAM2, OsBAM3, OsBAM4 and OsBAM5, which have a putative chloroplast transit peptide in their N-terminal regions, were mainly detected in leaf blades, leaf sheaths and internodes after heading. Soluble protein fractions prepared from Escherichia con containing recombinant OsBAM2 and OsBAM3 proteins had significant beta-amylase activity. Furthermore, OsBAM2-GFP and OsBAM3-GFP fusion proteins introduced into epidermis of Allium cepa scaly bulb were localized to the plastids. These results strongly suggest that OsBAM2 and OsBAM3 are plastid-targeted active beta-amylase.

    CROP SCIENCE SOC JAPAN, 2011年10月, PLANT PRODUCTION SCIENCE, 14 (4), 318 - 324, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoshi Kubo, Takehiro Masumura, Yuhi Saito, Hiroshi Fukayama, Yuji Suzuki, Toshio Sugimoto, Amane Makino, Katsumi Amako, Chikahiro Miyake

    We investigated the regulation mechanism of cyclic electron flow around photosystem I (CEF-PSI) in the rice leaves which suffered from photosystem II (PSII) photoinhibition. High-light (HL) treatment [2000 mu mol photon m-2 s-1 at 0% carbon dioxide (CO2), 2% oxygen (O2) and 25 degrees C] of rice leaves decreased both the maximum quantum efficiency of PSII (Fv/Fm) and the light-dependent O2-evolution rate [V(O2)]. High-light treatment did not affect the relative electron flux in PSI [phi(PSI) x PFD]. In non-treated leaves, increasing in the photon flux density (PFD) enhanced V(O2), phi(PSI) x PFD and the ratio of oxidized P700 to total P700 [(P700+)/(P700)total]. phi(PSI) x PFD continued to increase even after the saturation of V(O2) against PFD. These results suggested that the electrons not used for the major electron sink, photosynthetic carbon reduction-cycle, did turnover in PSI, that is, CEF-PSI functioned at a higher PFD. High-light treatments did not affect the activity of CEF-PSI and increased (P700+)/(P700)total in the lower PFDs, compared to non-treated leaves. The activity of CEF-PSI depends on the amount of oxidized PQ. Photoinhibition of PSII suppressed electron influx from PSII to photosynthetic linear electron transport. The enhanced (P700+)/(P700)total suggested the increase in the ratio of oxidized plastoquinone (PQ) to total PQ, which supported the activity of CEF-PSI in the photoinhibited leaves.

    WILEY-BLACKWELL, 2011年02月, SOIL SCIENCE AND PLANT NUTRITION, 57 (1), 105 - 113, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Gene expression profiling of rice grown in free air CO2 enrichment (FACE) and elevated soil temperature

    深山 浩, 杉野 充保, Fukuda T, Masumoto C, Taniguchi Y, Okada M, Sameshima R, 畠中 知子, 三十尾 修司

    2011年, Field Crops Res., 121, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Functional Incorporation of Sorghum Small Subunit Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice.

    石川 知恵, 畠中 知子, 三十尾 修司, 三宅 親弘, 深山 浩

    2011年, Plant Physiol., 156, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Functional Incorporation of Sorghum Small Subunit Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice

    Ishikawa C, Hatanaka T, Misoo S, Miyake C, Fukayama H

    American Society of Plant Biologists, 2011年, Plant Physiology, 156, 1603 - 1611, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Activation of cyclic electron flow around PSI in the photoinhibited rice leaves

    Satoshi Kubo, Takehiro Masumura, Yuhi Saito, Hiroshi Fukayama, Yuji Suzuki, Toshio Sugimoto, Amane Makino, Katsumi Amako, Chikahiro Miyake

    2011年, Soil Science and Plant Nutrition, 57, 105 - 113, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Fukayama, Rie Abe, Naotsugu Uchida

    Protease activities and its relation to the contents of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase were investigated in detached leaves of rice (Oryza sativa L) floated on the solutions containing abscisic acid (ABA) or benzyladenine (BA). Rubisco and Rubisco activase contents were decreased during the time course and the decreases were enhanced by ABA and suppressed by BA. The decrease in Rubisco activase was faster than that in Rubisco. SDS-dependent protease activities at 50 -70 kDa (rice SDS-dependent protease: RSP) analyzed by the gelatin containing PAGE were significantly enhanced by ABA. RSPs were also increased in attached leaves during senescence. RSPs had the pH optimum of 5.5, suggesting that RSPs are vacuolar protease. Both decrease in Rubisco and Rubisco activase contents and increase in RSPs activities were suppressed by cycloheximide. These findings indicate that the activities of RSPs are well correlated with the decrease in these protein contents. Immunoblotting analysis showed that Rubisco in the leaf extracts was completely degraded by 5 h at pH 5.5 with SDS where it was optimal condition for RSPs. However, the degradation of Rubisco did not proceed at pH 7.5 without SDS where it is near physiological condition for stromal proteins. Rubisco activase was degraded at similar rate under both conditions. These results suggest that RSPs can functions in a senescence related degradation system of chloroplast protein in rice leaves. Rubisco activase would be more susceptible to proteolysis than Rubisco under physiological condition and this could affect the contents of these proteins in leaves. (C) 2010 Elsevier Masson SAS. All rights reserved.

    ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2010年10月, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 48 (10-11), 808 - 812, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Screening of High k(cat) Rubisco among Poaceae for Improvement of Photosynthetic CO2 Assimilation in Rice

    Chie Ishikawa, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a major limiation of photosynthetic CO2 assimilation in C-3 plants. In order to find useful Rubisco for improvement of photosynthesis in rice under elevated CO2, we analyzed the catalytic turnover rate (k(cat)) of Rubisco in Poaceae including C-3 alpine plants, C-3 cold-resistant plants and C-4 plants. Rubisco in these plants showed 1.1- to 2.8-fold higher k(cat) than that in rice. However, the most of high k(cat) Rubisco also showed a higher km for CO2 (Kc) than that of rice, indicating that increase in k(cat) led to decrease in the affinity for CO2. Rubisco in Festuca ovina, Phleum pratense and Sorghum bicolor showed relatively high k(cat) to Kc. Although the k(cat) of Rubisco in F. ovina and P. pralense was not so high (1.5-1.6 fold relative to rice), the Kc was comparable to that in rice and the amino acid sequence of RbcL shared higher identity to that in rice than that in S. bicolor. By contrast, Rubisco of S. bicolor showed considerably high k(cat) (2.5-fold relative to rice), which is considered to be the most improtant factor for improvement of photosynthesis. In our estimation, the expression of high k(cat) Rubisco of F. ovina and S. bicolor in rice could significantly enhance CO2 assimilation at Ci and 50 Pa, the level assumed to be the middle of this century.

    CROP SCIENCE SOC JAPAN, 2009年07月, PLANT PRODUCTION SCIENCE, 12 (3), 345 - 350, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Phosphoenolpyruvate carboxylase intrinsically located in the chloroplast of rice plays a crucial role in ammonium assimilation

    Chisato Masumoto, Shinichi Miyazawa, Hiroshi Ohkawa, Takuya Fukuda, Yojiro Taniguchi, Seiji Murayama, Miyako Kusano, Kazuki Saito, Hiroshi Fukayama, Mitsue Miyao

    2009年, Proceedings of the National Academy of Sciences of the United States of America, 177, 203 - 210, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Identification of C4 responsive genes in the facultative C4 plant Hydrilla verticillata.

    Hiroshi Fukayama

    2006年07月, Photosynthesis Research, 88, 173-183,, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Characterization and functional analysis of phosphoenolpyruvate carboxylase kinase genes in rice.

    Hiroshi Fukayama

    2006年04月, The Plant Journal, 47, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • 植物の高CO2応答における遺伝子発現変化

    深山 浩

    2013年, 化学と生物, 51, 710 - 716, 日本語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • 高CO2環境に適したRubiscoの導入によるイネの光合成能力の改良

    深山 浩

    2013年, 光合成研究, 23, 24 - 32, 日本語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • 光合成誘導反応

    唐 艶鴻, 冨松 元, 深山 浩

    2013年, 化学と生物, 52, 106 - 112, 日本語

    [査読有り][招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • Mitsue Miyao, Chisato Masumoto, Shin-Ichi Miyazawa, Hiroshi Fukayama

    The transfer of C-4 plant traits into C-3 plants has long been a strategy for improving the photosynthetic performance of C-3 plants. The introduction of a pathway mimicking the C-4 photosynthetic pathway into the mesophyll cells of C-3 plants was only a realistic approach when transgenic technology was sufficiently well developed and widely adopted. Here an attempt to introduce a single-cell C-4-like pathway in which CO2 capture and release occur in the mesophyll cell, such as the one found in the aquatic plant Hydrilla verticillata (L.f.) Royle, into rice (Oryza sativa L.) is described. Four enzymes involved in this pathway were successfully overproduced in the transgenic rice leaves, and 12 different sets of transgenic rice that overproduce these enzymes independently or in combination were produced and analysed. Although none of these transformants has yet shown dramatic improvements in photosynthesis, these studies nonetheless have important implications for the evolution of C-4 photosynthetic genes and their metabolic regulation, and have shed light on the unique aspects of rice physiology and metabolism. This article summarizes the lessons learned during these attempts to engineer single-cell C-4 rice.

    OXFORD UNIV PRESS, 2011年05月, JOURNAL OF EXPERIMENTAL BOTANY, 62 (9), 3021 - 3029, 英語

    [査読有り]

    書評論文,書評,文献紹介等

講演・口頭発表等

  • Vernonia galamensis DGAT1は他種のDGAT1より シロイヌナズナ種子油含量を上げることができる

    畠中 知子, 冨田 典希, 周 采穎, 笹山 大輔, 深山 浩, 東 哲司, DAVID F. HILDEBRAND

    第31回植物脂質シンポジウム, 2018年11月, 日本語, 高知大学, 国内会議

    ポスター発表

  • Vernonia galamensis DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s

    畠中 知子, 冨田 典希, 周 采穎, 笹山 大輔, 深山 浩, 東 哲司, DAVID F. HILDEBRAND

    The 14th International Symposium on Biocatalysis and Agricultural Biotechnology, 2018年10月, 英語, Kuala Lumpur, Malaysia, 国際会議

    ポスター発表

  • 南米野生イネOryza glumaepatulaの浮稲反応の解析

    新川 麻由子, 遠藤 宏朗, 笹山 大輔, 畠中 知子, 深山 浩, 東 哲司

    日本作物学会第246回講演会, 2018年09月, 日本語, 北海道大学農学部, 国内会議

    ポスター発表

  • イネRubiscoのC4化と構造機能相関

    塩見 慶太, 笹山 大輔, 畠中 知子, 東 哲司, 吉川 大貴, 松村 浩由, 深山 浩

    日本作物学会第246回講演会, 2018年09月, 日本語, 北海道大学農学部, 国内会議

    口頭発表(一般)

  • アフリカイネOryza glaberrimaとその祖先種Oryza barthiiの洪水に対する応答

    羅 全淑, 中澤 美沙希, 笹山 大輔, 畠中 知子, 深山 浩, 東 哲司

    日本作物学会第246回講演会, 2018年09月, 日本語, 北海道大学農学部, 国内会議

    ポスター発表

  • Highly active Vernonia galamensis DGAT1 can effectively increase oil levels in yeast, soybean and Arabidopsis

    畠中 知子, 笹山 大輔, 深山 浩, 東 哲司, 松岡 大介, 冨田 典希, 周 采穎, 宮下 稚菜, William Serson, Runzhi Li, Paul Armstrong, Keshun Yu, Todd Pfeiffer, Xi-Le Li, David F. Hildebrand

    The 23rd International Symposium on Plant Lipids, 2018年07月, 英語, Yokohama, Japan, 国際会議

    ポスター発表

  • アフリカイネ Oryza glaberrima とその祖先種 O. barthii の洪水に対する応答

    羅 全淑, 中澤 美沙希, 笹山 大輔, 畠中 知子, 深山 浩, 東 哲司

    近畿作物・育種研究会 第185回例会, 2018年06月, 日本語, 国内会議

    口頭発表(一般)

  • バレイショにおけるイネCRCT遺伝子のオーソログの解析

    林龍之介, 横山音, 津釜大輔, 森田隆太郎, 深山浩, 藤野介延

    日本作物学会講演会, 2018年, 日本語, 国内会議

    ポスター発表

  • シロイヌナズナにおける CRCT オーソログの機能解析

    萩原輝, 中馬望, 松岡大介, 笹山大輔, 畠中知子, 東哲司, 深山浩

    日本作物学会講演会, 2018年, 日本語, 国内会議

    ポスター発表

  • イネの Rubisco activase 様タンパク質 Rca2 は Rubisco activase の機能を阻害する

    水本晃那, 小林亜希子, 山内靖雄, 松岡大介, 笹山大輔, 畠中知子, 東哲司, 深山浩

    日本作物学会講演会, 2018年, 日本語, 国内会議

    ポスター発表

  • イネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害する

    水本晃那, 小林亜希子, 松岡大介, 山内靖雄, 笹山大輔, 畠中知子, 東哲司, 深山浩

    日本光合成学会講演会, 2018年, 日本語, 国内会議

    ポスター発表

  • Introduction of C4-like enzymic properties and nitrogen distribution of Rubisco into C3 plant, rice

    深山浩, 山本滉樹人, 塩見慶太, 小林則之, 吉川大貴, 谷口洋二郎, 松村浩由

    日本植物生理学会年会, 2018年, 英語, 国内会議

    シンポジウム・ワークショップパネル(指名)

  • Functional analysis of Rubisco small subunit, OsRbcS1, that is not expressed in photosynthetic organs in rice

    小林 則之, 森田 耕一, 笹山 大輔, 畠中 知子, 東 哲司, 深山 浩

    International Symposium on Photosynthesis and Chloroplast Biogenesis, 2018年, 英語, 国内会議

    ポスター発表

  • C4植物の Rubisco 小サブユニットを高発現する形質転換イネの Rubisco 酵素特性

    小原崇, 笹山大輔, 畠中知子, 東哲司, 深山浩

    日本作物学会講演会, 2018年, 日本語, 国内会議

    ポスター発表

  • Highly active Vernonia galamensis DGAT1 can effectively increase seed oil levels in soybean and Arabidopsis

    畠中 知子, 冨田 典希, 周 采穎, 笹山 大輔, 深山 浩, 東 哲司, William Serson, Runzhi Li, Paul Armstrong, Keshun Yu, Todd Pfeiffer, Xi-Le Li, David F. Hildebrand

    The 7th Asian Symposium on Plant Lipid, 2017年11月, 英語, Taipei, Taiwan, 国際会議

    ポスター発表

  • 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ品種の深水成長応答と関連遺伝子の発現

    尾江 俊祐, 笹山 大輔, 深山 浩, 畠中 知子, 東 哲司

    日本作物学会第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議

    ポスター発表

  • 南米野生イネOryza glumaepatulaの深水に対する応答とSNORKEL遺伝子の多様性

    遠藤 宏朗, 新川 麻由子, 笹山 大輔, 畠中 知子, 深山 浩, 東 哲司

    日本作物学会第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議

    ポスター発表

  • 深水環境下において浮稲節間の伸長を誘導する新規因子の探索

    冨田 一嘉, 笹山 大輔, 深山 浩, 畠中 知子, 東 哲司

    日本作物学会第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議

    ポスター発表

  • ベルノニアのDGAT1はシロイヌナズナにおいても他種のDGAT1より高い活性を示すか?

    畠中 知子, 冨田 典希, 周 采穎, 笹山 大輔, 深山 浩, 東 哲司, David F. Hildebrand

    日本作物学会 第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議

    ポスター発表

  • シロイヌナズナにおけるベルノニアDGAT1の働き

    畠中 知子, 冨田 典希, 周 采穎, 笹山 大輔, 深山 浩, 東 哲司, David F. Hildebrand

    第30回植物脂質シンポジウム, 2017年09月, 日本語, お茶の水大学, 国内会議

    ポスター発表

  • Vernonia DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s

    畠中 知子, 冨田 典希, 周 采穎, 笹山 大輔, 深山 浩, 東 哲司, David F. Hildebrand

    Plant Biology 2017, 2017年06月, 英語, Honolulu, Hawaii, USA, Plant oils represent one of the world’s most important renewable resources. In addition to extensive food uses, vegetable oils also have value as renewable chemical derivatives and fuels, such as biodiesel. Worldwide production of plant oil has been increasing. Triacylglycerols (TAGs) are the major component of plant storage lipid. Acyl-CoA:diacylglycerol acyltransferase (DGAT), 国際会議

    ポスター発表

  • マイクロアレイ法によるOsRbcS1発現抑制イネの遺伝子発現プロファイリング

    森田 耕一, 笹山 大輔, 東 哲司, 深山 浩

    日本作物学会講演会, 2017年, 日本語, 国内会議

    ポスター発表

  • デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果

    森田 隆太郎, 寺村 浩, 荻野 千秋, 近藤 昭彦, 深山 浩

    日本作物学会講演会, 2017年, 日本語, 国内会議

    ポスター発表

  • ソルガム高活性型Rubiscoの小サブユニットを利用したイネの光合成能力の改良

    塩見慶太, 山本滉樹人, 畠中知子, 笹山大輔, 東哲司, 深山浩

    農芸化学会関西支部会, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • イネにおけるRubisco activase様タンパク質OsRca2の機能解析

    水本晃那, 小林亜希子, 畠中知子, 笹山大輔, 東哲司, 深山浩

    農芸化学会関西支部会, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • CO2応答性CCTタンパク質CRCTはイネ栄養器官のデンプン合成制御に働く

    森田 隆太郎, 深山 浩

    日本作物学会講演会, 2017年, 日本語, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の深水伸長応答

    尾江 俊佑, 笹山 大輔, 深山 浩, 東 哲司

    日本作物学会 第242回講演会, 2016年09月, 日本語, 龍谷大学瀬田キャンパス, 国内会議

    ポスター発表

  • 水田雑草オモダカとウリカワの塊茎萌芽期の節間伸長の酸素欠乏および二酸化炭素による促進

    笹山 大輔, 深山 浩, 伊藤 一幸, 東 哲司

    日本作物学会 第242回講演会, 2016年09月, 日本語, 龍谷大学瀬田キャンパス, 国内会議

    口頭発表(一般)

  • エチレンとジベレリンが誘導する浮稲節間の伸長促進に及ぼす重力方向と過重力の影響

    中谷 友美, 笹山 大輔, 深山 浩, 東 哲司

    日本作物学会 第242回講演会, 2016年09月, 日本語, 龍谷大学瀬田キャンパス, 国内会議

    ポスター発表

  • 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の生理特性

    尾江 俊佑, 笹山 大輔, 深山 浩, 東 哲司

    近畿作物・育種研究会 第181回例会, 2016年05月, 日本語, 国内会議

    口頭発表(一般)

  • 多年生水田雑草オモダカとウリカワの節間伸長はそれぞれ酸素欠乏と二酸化炭素によって促進される

    笹山 大輔, 深山 浩, 伊藤 一幸, 東 哲司

    近畿作物・育種研究会 第181回例会, 2016年05月, 日本語, 滋賀県立大学 サテライト教室, 国内会議

    口頭発表(一般)

  • 高等植物の貯蔵脂質合成の強化に関する研究

    冨田 典希, 木村 円香, 深山 浩, 三十尾 修司, 畠中 知子

    日本作物学会第241回講演会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • 高CO2条件で育成したソルガムRbcS高発現・イネRbcS発現抑制2重形質転換イネの生理特性

    山本 滉樹人, 迫田 和馬, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会講演会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • デンプン合成関連酵素の発現制御に働くCRCTは葉鞘のデンプンの鎖長分布パターンに影響を与える

    森田 隆太郎, クロフツ 尚子, 畠中 知子, 三十尾 修司, 藤田 直子, 深山 浩

    日本作物学会講演会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • シロイヌナズナにおけるCRCTオーソログの発現解析

    萩原 輝, 中馬 望, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会講演会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • イネ葉身の発達・老化過程におけるOsRca2 含量の変化

    水本 晃那, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会講演会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • OsRbcS1の代謝機能:植物におけるOsRbcS1様RbcSの発現解析とOsRbcS1発現抑制イネのメタボローム解析

    森田 耕一, 畠中 知子, 三十尾 修司, 草野 都, 深山 浩

    日本作物学会講演会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • CO2 Responsive CCT protein alters the chain-length distribution of starch from leaf sheath in rice by regulating the expression of multiple starch synthesis related enzymes

    森田 隆太郎, クロフツ尚子, 畠中 知子, 三十尾 修司, 藤田 直子, 深山 浩

    日本植物生理学会年会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • Starch Content in Leaf Sheath Controlled by CO2 Responsive CCT Protein is a potential Determinant of Photosynthetic Capacity in Rice Grown under Elevated CO2 Condition

    Ryutaro Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    The 17th International Congress on Photosynthesis Research, 2016年, 英語, 国際会議

    ポスター発表

  • Functional analysis of OsRbcS1, a non-photosynthetic small subunit that increases the catalytic turnover rate of Rubisco in rice

    Koichi Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    The 17th International Congress on Photosynthesis Research, 2016年, 英語, 国際会議

    ポスター発表

  • Engineering Rubisco and starch synthesis to enhance photosynthesis under elevated CO2

    深山 浩

    The 2015 Tokyo Whole Plant Photosynthesis Workshop, 2015年05月, 英語, 国内会議

    口頭発表(招待・特別)

  • Sink Capacity of Leaf Sheath Regulated by CRCT Affects the Photosynthetic Rate of Leaf Blade in Rice Grown under Elevated CO2 Condition.

    森田 隆太郎, 畠中 知子, 三十尾 修司, 深山 浩

    日本植物生理学会年会, 2015年03月, 日本語, 国内会議

    口頭発表(一般)

  • Responsive CCT Protein Ortholog in Arabidopsis Controls the Starch Accumulation in Leaves.

    中馬 望, 畠中 知子, 三十尾 修司, 深山 浩

    日本植物生理学会年会, 2015年03月, 日本語, 国内会議

    ポスター発表

  • Functional analysis of Rubisco activase-like protein, OsRca2 in rice.

    小林 亜希子, 畠中 知子, 三十尾 修司, 深山 浩

    日本植物生理学会年会, 2015年03月, 日本語, 国内会議

    口頭発表(一般)

  • CO2 Responsive CCT Protein, CRCT Is a Positive Regulator of Starch Synthesis in Vegetative Organ in Rice.

    高堂 愛子, 森田 隆太郎, 畠中 知子, 三十尾 修司, 深山 浩

    日本植物生理学会年会, 2015年03月, 日本語, 国内会議

    ポスター発表

  • A rice MAP3K, Os11g45280, controls plant growth concerning tiller formation

    山村 優太, 深山 浩, 松岡 大介, 南森 隆司

    第56回日本植物生理学会年会, 2015年03月, 日本語, 国内会議

    ポスター発表

  • Rubisco activase様タンパク質OsRca2の光合成における機能

    深山 浩, 小林 亜希子, 水本晃那, 畠中 知子, 三十尾 修司

    日本作物学会講演会, 2015年, 日本語, 国内会議

    口頭発表(一般)

  • 耐塩性インディカイネ交雑由来倍加半数体系統の継続的耐塩性評価

    板倉俊介, 深山 浩, 畠中 知子, 三十尾 修司

    日本作物学会, 2014年09月, 日本語, 愛媛大学, 国内会議

    口頭発表(一般)

  • デンプン合成の制御因子CRCTの発現量がイネの生育と光合成に与える影響

    森田 隆太郎, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会講演会, 2014年09月, 日本語, 国内会議

    ポスター発表

  • イネ高活性型Rubisco小サブユニット遺伝子OsRbcS1のオーソログの発現解析

    森田 耕一, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会講演会, 2014年09月, 日本語, 国内会議

    ポスター発表

  • ダイズ体細胞胚長期懸濁培養におけるマルトースの効果に関する一考察

    畠中 知子, 木原 由貴, 左海 庸光, 瀬田 聡美, 深山 浩, 三十尾 修司

    第177回 近畿作物・育種研究会例会, 2014年07月, 日本語, 神戸大学農学部, 国内会議

    口頭発表(一般)

  • 高CO2条件下で発現促進されるCCTタンパク質CRCTはイネの栄養器官におけるデンプン合成の調節因子である

    森田 隆太郎, 杉野 充保, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議

    口頭発表(一般)

  • 寒地型イネ科牧草チモシーのRbcSを高発現する形質転換イネの光合成特性.

    古賀 敦士, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議

    口頭発表(一般)

  • ソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法でRubisco含量を減少させた二重形質転換イネの光合成特性.

    迫田 和馬, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議

    口頭発表(一般)

  • イネ高活性型Rubisco小サブユニットOsRbcS1の機能解析

    森田 耕一, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議

    口頭発表(一般)

  • イネのPASドメインを有するMAP3Kの機能について

    高岡 翔平, 深山 浩, 松岡 大介, 南森 隆司

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 国内会議

    口頭発表(一般)

  • C3植物のC4化:Rubiscoだけでも役に立つ?

    深山 浩

    日本植物学会, 2014年, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 耐塩性イネ品種PokkaliのF1倍加半数体系統群における幼苗期耐塩性指標の評価

    梅嵜友理香, 西村幸一郎, 深山 浩, 畠中 知子, 三十尾 修司

    日本作物学会, 2013年, 日本語, 国内会議

    口頭発表(一般)

  • 高CO2条件で発現促進される新規CCTドメインタンパク質CRCTは分げつ角度と基本代謝酵素の発現調節に働く

    森田隆太郎, 杉野充保, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2013年, 日本語, 国内会議

    口頭発表(一般)

  • ソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法等でRubisco量を減少させた形質転換イネの生理特性

    深山 浩, 石川智恵, 畠中 知子, 三十尾 修司

    日本作物学会, 2013年, 日本語, 国内会議

    口頭発表(一般)

  • イネにおいて葉身で発現しない新規Rubisco小サブユニットOsRbcS1の機能解析

    森田耕一, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2013年, 日本語, 国内会議

    口頭発表(一般)

  • Rubisco activaseはRubisco生合成を阻害することによりRubiscoの発現を抑制する

    上口千晶, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2013年, 日本語, 国内会議

    口頭発表(一般)

  • 幼苗期におけるインディカイネの交雑親と倍加半数体系統群の耐塩性比較

    西村 幸一郎, 深山 浩, 畠中 知子, 三十尾 修司

    日本作物学会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • 高CO2条件で発現促進される受容体型キナーゼOsTPK1の機能解析

    廣瀬 由依, 畠中 知子, 三十尾 修司, 深山 浩

    日本作物学会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • 高CO2 環境に適したRubisco の導入によるイネの光合成能力の改良

    深山 浩

    本光合成学会 公開シンポジウム「植物とCO2」, 2012年, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • イネにおいて高CO2条件下で発現促進される新規CCTタンパク質OsCCT1の機能解析

    深山 浩, 森田隆太郎, 杉野充保, 畠中 知子, 三十尾 修司

    日本植物生理学会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • 新規なCCTドメインタンパク質OsCCT1は高CO2環境への代謝的・形態的応答に関係している.

    杉野充保, 深山 浩, 酒井英光, 畠中 知子, 三十尾 修司

    日本作物学会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • イネにおける葉緑体型ホスホグリセリン酸ムターゼOsPGMd2の機能解析

    加藤祐樹, 深山 浩, 大河浩, 谷口洋二郎, 増本千都, 宮尾光恵, 畠中 知子, 三十尾 修司

    日本作物学会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • Rubisco activaseの高発現はRubisco含量の減少により光合成速度を低下させる

    深山 浩, 上口千晶, 西川薫, 石川智恵, 畠中 知子, 三十尾 修司

    日本植物生理学会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • ソルガムRbcS の高発現は形質転換イネにおけるRubisco の反応回転速度を増加させる

    石川智恵, 畠中 知子, 三十尾 修司, 深山 浩

    日本植物生理学会年会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • イネにおけるプラスチド局在型エノラーゼ遺伝子の機能解析

    深山 浩, 加藤祐樹, 新谷翔太, 谷口洋二郎, 増本千都, 畠中 知子, 三十尾 修司, 宮尾光恵, 大河浩

    日本作物学会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • Chimeric Incorporation of Sorghum RbcS Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice Plants

    Chie Ishikawa, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

    The 15th International Congress of Photosynthesis, 2010年, 英語, 国際会議

    口頭発表(一般)

  • オオムギRubisco activaseの高発現がイネの光合成特性と葉内成分含量におよぼす効果

    深山 浩, 西川 薫, 畠中 知子, 三十尾 修司

    日本作物学会大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 油料作物Jatropha curcasの組織培養による増殖と脂質定量

    栗本 温子, 畠中 知子, 深山 浩, 三十尾 修司, 三本木 一夫

    日本作物学会大会, 2008年, 日本語, 国内会議

    ポスター発表

  • 油料作物Jatropha curcasの脂質含量と組織培養による増殖

    栗本 温子, 畠中 知子, 深山 浩, 三十尾 修司, 三本木 一夫

    近畿作物・育種研究会例会, 2008年, 日本語, 神戸大学, 国内会議

    口頭発表(一般)

  • 丹波黒大豆‘兵系黒3号’の成熟培養における,糖とアミノ酸の効果

    木原 由貴, 畠中 知子, 深山 浩, 三十尾 修司

    日本作物学会大会, 2008年, 日本語, 国内会議

    ポスター発表

  • 開放系大気CO2 濃度増加条件(FACE)で育成したイネの遺伝子発現プロファイリング

    深山 浩, 福田 琢哉, 長谷川 利拡, 宮尾 光恵, 畠中 知子, 三十尾 修司

    近畿作物・育種研究会例会, 2008年, 日本語, 神戸大学, 国内会議

    口頭発表(一般)

  • イネの光合成能力改良に向けたイネ科植物内における高Kcat Rubiscoの探索

    深山 浩, 石川 智恵, 畠中 知子, 三十尾 修司

    日本作物学会大会, 2008年, 日本語, 国内会議

    口頭発表(一般)

  • Ribulose-1,5-bisphosphate carboxylase/oxygenase activaseを過剰発現させた形質転換イネの光合成特性

    増本 千都, 山田 健太郎, 加藤 伸明, 深山 浩, 畠中 知子, 内田 直次

    日本作物学会大会, 2007年, 日本語, 国内会議

    口頭発表(一般)

  • Ribulose-1,5-bisphosphate carboxylase/oxygenase acitivase (Rubisco activase)含量を減少させた形質転換イネの光強度に対する光合成反応

    増本 千都, 畠中 知子, 深山 浩, 内田 直次

    日本作物学会大会, 2006年, 日本語, 国内会議

    ポスター発表

所属学協会

  • 日本光合成研究会

  • 日本植物生理学会

  • 日本作物学会

共同研究・競争的資金等の研究課題