研究者紹介システム

土佐 幸雄
トサ ユキオ
大学院農学研究科 生命機能科学専攻
教授
農学部長;大学院農学研究科長
農学関係
Last Updated :2021/04/12

研究者情報

所属

  • 【主配置】

    大学院農学研究科 生命機能科学専攻
  • 【配置】

    農学部 生命機能科学科

学位

  • 農学博士, 京都大学

授業科目

ミニ講義

ジャンル

  • 環境・交通 / 農業

コメントテーマ

  • 植物病理学
  • いもち病菌
  • 細胞死機構

研究ニュース

研究活動

研究キーワード

  • 植物病理学

研究分野

  • 環境・農学 / 植物保護科学
  • 環境・農学 / 植物保護科学

受賞

  • 2009年03月 日本植物病理学会, 日本植物病理学会賞, 植物病原糸状菌における種特異的寄生性の遺伝学的解析

    土佐 幸雄

    日本国

    国内学会・会議・シンポジウム等の賞

  • 1993年04月 日本植物病理学会, 日本植物病理学会学術奨励賞, ムギ類うどんこ病菌の属間寄生性の分化機構に関する研究

    土佐 幸雄

    国内学会・会議・シンポジウム等の賞

論文

  • Evolution of an Eleusine-Specific Subgroup of Pyricularia oryzae Through a Gain of an Avirulence Gene

    Asuke S., Tanaka M., Hyon GS., Inoue Y., Vy TTP., Niwamoto D., Nakayashiki H., Tosa Y.

    2020年02月, Mol Plant Microbe Interact., 33 (2), 153 - 165, 英語

    [査読有り]

    研究論文(学術雑誌)

  • At Least Five Major Genes Are Involved in the Avirulence of an Eleusine Isolate of Pyricularia oryzae on Common Wheat

    Asuke S., Nishimi S., Tosa Y.

    2020年, Phytopathology, 110 (2), 465 - 471, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Blast fungal genomes show frequent chromosomal changes, gene gains and losses, and effector gene turnover

    Luis B, Gómez Luciano, Isheng Jason Tsai, Izumi Chuma, 土佐 幸雄, Yi-Hua Chen, Jeng-Yi Li, Meng-Yun Li, Mei-Yeh Jade Lu, 中屋敷 均, Wen-Hsiung Li

    Oxford press, 2019年03月, Molecular Biology and Evolution, 36, 1148 - 1161, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mosquito larvicidal and antifungal isoquinoline alkaloids from Papaveraceae

    Ueno, S., Park, P., Tosa, Y., Maoka, T., Kojima, N., Yamashita, M., Inoue, M., , Ueno, T.

    2019年, Jpn. J. Environ. Entomol. Zool., 30, 51 - 61, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Vu Lan Anh, Yoshihiro Inoue, Soichiro Asuke, Trinh Thi Phuong Vy, Nguyen Tuan Anh, Shizhen Wang, Izumi Chuma, Yukio Tosa

    Rmg8 and Rmg7 are genes for resistance to the wheat blast fungus (Pyricularia oryzae), located on chromosome 2B in hexaploid wheat and chromosome 2A in tetraploid wheat, respectively. AVR-Rmg8, an avirulence gene corresponding to Rmg8, was isolated from a wheat blast isolate through a map-based strategy. The cloned fragment encoded a small protein containing a putative signal peptide. AVR-Rmg8 was recognized not only by Rmg8, but also by Rmg7, suggesting that these two resistance genes are equivalent to a single gene from the viewpoint of resistance breeding.

    Blackwell Publishing Ltd, 2018年05月01日, Molecular Plant Pathology, 19 (5), 1252 - 1256, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A new resistance gene in combination with Rmg8 confers strong resistance against Triticum isolates of Pyricularia oryzae in a common wheat landrace

    Shizhen Wang, 足助 聡一郎, Trinh Thi Phuong, 井上 喜博, 中馬 いづみ, Joe Win, 加藤 鎌司, 土佐 幸雄

    2018年, Phytopathology, 108 (11), 1299 - 1306, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yoshihiro Inoue, Trinh T. P. Vy, Kentaro Yoshida, Hokuto Asano, Chikako Mitsuoka, Soichiro Asuke, Vu L. Anh, Christian J. R. Cumagun, Izumi Chuma, Ryohei Terauchi, Kenji Kato, Thomas Mitchell, Barbara Valent, Mark Farman, Yukio Tosa

    Wheat blast first emerged in Brazil in the mid-1980s and has recently caused heavy crop losses in Asia. Here we show how this devastating pathogen evolved in Brazil. Genetic analysis of host species determinants in the blast fungus resulted in the cloning of avirulence genes PWT3 and PWT4, whose gene products elicit defense in wheat cultivars containing the corresponding resistance genes Rwt3 and Rwt4. Studies on avirulence and resistance gene distributions, together with historical data on wheat cultivation in Brazil, suggest that wheat blast emerged due to widespread deployment of rwt3 wheat (susceptible to Lolium isolates), followed by the loss of function of PWT3. This implies that the rwt3 wheat served as a springboard for the host jump to common wheat.

    AMER ASSOC ADVANCEMENT SCIENCE, 2017年07月, SCIENCE, 357 (6346), 80 - 82, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Analiza Grubanzo Tagle, Izumi Chuma, Hiroshi Hisano, Kazuhiro Sato, Yukio Tosa

    The resistance of barley to Pyricularia oryzae isolates is controlled by the Rmo2 locus irrespective of their original hosts. The resistance of barley cultivar H.E.S.4 to isolate tO-7 of P. pennisetigena (a cryptic species in the P. oryzae/grisea species complex) cosegregated with the resistance to P. oryzae isolate GFSI-1-7-2 controlled by Rmo2.a. On the other hand, its resistance to isolate NI919, belonging to another cryptic species of Pyricularia, was controlled by another gene inherited independently from Rmo2.a. These results suggest that gene-for-gene interactions underlie the resistance of barley to cryptic species of Pyricularia.

    SPRINGER JAPAN KK, 2016年11月, JOURNAL OF GENERAL PLANT PATHOLOGY, 82 (6), 302 - 306, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ning Zhang, Jing Luo, Amy Y. Rossman, Takayuki Aoki, Izumi Chuma, Pedro W. Crous, Ralph Dean, Ronald P. de Vries, Nicole Donofrio, Kevin D. Hyde, Marc-Henri Lebrun, Nicholas J. Talbot, Didier Tharreau, Yukio Tosa, Barbara Valent, Zonghua Wang, Jin-Rong Xu

    The order Magnaporthales comprises about 200 species and includes the economically and scientifically important rice blast fungus and the take-all pathogen of cereals, as well as saprotrophs and endophytes. Recent advances in phylogenetic analyses of these fungi resulted in taxonomic revisions. In this paper we list the 28 currently accepted genera in Magnaporthales with their type species and available gene and genome resources. The polyphyletic Magnaporthe 1972 is proposed for suppression, and Pyricularia 1880 and Nakataea 1939 are recommended for protection as the generic names for the rice blast fungus and the rice stem rot fungus, respectively. The rationale for the recommended names is also provided. These recommendations are made by the Pyricularia/Magnaporthe Working Group established under the auspices of the International Commission on the Taxonomy of Fungi (ICTF).

    INT MYCOLOGICAL ASSOC, 2016年06月, IMA FUNGUS, 7 (1), 155 - 159, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naho Yamagishi, Toyozo Sato, Izumi Chuma, Yoshiyuki Ishiyama, Yukio Tosa

    Brown spots were found on leaves of black locust (Robinia pseudoacacia L.) in Nagano Prefecture, Japan, in August 2011. A fungus isolated from the diseased leaves reproduced the symptoms after inoculation, and was re-isolated from the lesions. The pathogen was identified as Colletotrichum nymphaeae based on morphological and molecular analyses. This is the first report of C. nymphaeae as a pathogen of anthracnose of black locust in Japan.

    SPRINGER JAPAN KK, 2016年05月, JOURNAL OF GENERAL PLANT PATHOLOGY, 82 (3), 174 - 176, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kentaro Yoshida, Diane G. O. Saunders, Chikako Mitsuoka, Satoshi Natsume, Shunichi Kosugi, Hiromasa Saitoh, Yoshihiro Inoue, Izumi Chuma, Yukio Tosa, Liliana M. Cano, Sophien Kamoun, Ryohei Terauchi

    Background: Magnaporthe oryzae (anamorph Pyricularia oryzae) is the causal agent of blast disease of Poaceae crops and their wild relatives. To understand the genetic mechanisms that drive host specialization of M. oryzae, we carried out whole genome resequencing of four M. oryzae isolates from rice (Oryza sativa), one from foxtail millet (Setaria italica), three from wild foxtail millet S. viridis, and one isolate each from finger millet (Eleusine coracana), wheat (Triticum aestivum) and oat (Avena sativa), in addition to an isolate of a sister species M. grisea, that infects the wild grass Digitaria sanguinalis. Results: Whole genome sequence comparison confirmed that M. oryzae Oryza and Setaria isolates form a monophyletic and close to another monophyletic group consisting of isolates from Triticum and Avena. This supports previous phylogenetic analysis based on a small number of genes and molecular markers. When comparing the host specific subgroups, 1.2-3.5 % of genes showed presence/absence polymorphisms and 0-6.5 % showed an excess of non-synonymous substitutions. Most of these genes encoded proteins whose functional domains are present in multiple copies in each genome. Therefore, the deleterious effects of these mutations could potentially be compensated by functional redundancy. Unlike the accumulation of nonsynonymous nucleotide substitutions, gene loss appeared to be independent of divergence time. Interestingly, the loss and gain of genes in pathogens from the Oryza and Setaria infecting lineages occurred more frequently when compared to those infecting Triticum and Avena even though the genetic distance between Oryza and Setaria lineages was smaller than that between Triticum and Avena lineages. In addition, genes showing gain/loss and nucleotide polymorphisms are linked to transposable elements highlighting the relationship between genome position and gene evolution in this pathogen species. Conclusion: Our comparative genomics analyses of host-specific M. oryzae isolates revealed gain and loss of genes as a major evolutionary mechanism driving specialization to Oryza and Setaria. Transposable elements appear to facilitate gene evolution possibly by enhancing chromosomal rearrangements and other forms of genetic variation.

    BIOMED CENTRAL LTD, 2016年05月, BMC GENOMICS, 17, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Vu Lan Anh, Nguyen Tuan Anh, Analiza Grubanzo Tagle, Trinh Thi Phuong Vy, Yoshihiro Inoue, Shigeo Takumi, Izumi Chuma, Yukio Tosa

    Blast, caused by Pyricularia oryzae, is one of the major diseases of wheat in South America. We identified a new gene for resistance to Triticum isolates of P. oryzae in common wheat 'S-615', and designated it "resistance to Magnaporthe grisea 8" (Rmg8). Rmg8 was assigned to chromosome 2B through molecular mapping with simple-sequence repeat markers. To identify an avirulence gene corresponding to Rmg8, Triticum isolate Br48 (avirulent on S-615) was crossed with 200R29 (virulent on S-615), an F-1 progeny derived from a cross between an Eleusine isolate (MZ5-1-6) and Br48. Segregation analysis of their progeny revealed that avirulence of Br48 on S-615 was conditioned by a single gene, which was designated AVR-Rmg8. AVR-Rmg8 was closely linked to AVR-Rmg7, which corresponded to Rmg7 located on chromosome 2A of tetraploid wheat.

    AMER PHYTOPATHOLOGICAL SOC, 2015年12月, PHYTOPATHOLOGY, 105 (12), 1568 - 1572, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naho Yamagishi, Masashi Fujinaga, Yoshiyuki Ishiyama, Hideki Ogiso, Toyozo Sato, Yukio Tosa

    Colletotrichum nymphaeae, causal agent of celery stunt anthracnose, has caused severe damage to celery production in Nagano Prefecture, Japan. For elucidating the infection cycle, celery seeds in the prefecture were screened on a selective medium and found to harbor C. nymphaeae as did seeds harvested from plants with diseased leaves. When seed samples with 1 and 10 % of the seeds infested were sown and grown, stunt anthracnose developed on seedlings, then finally spread extensively to other plants, suggesting that primary transmission occurred from seeds to seedlings, followed by secondary infection to other plants. These results strongly suggest that the disease cycle of the celery stunt anthracnose is completed through seed transmission in Nagano Prefecture. To disrupt the infection cycle, seed sterilization with hot water was tested. Conidia of C. nymphaeae in suspensions were killed by treatment at 50 A degrees C for 40 min or at > 55 degrees C for 10 min. Seedlings grown from seeds treated at 50 degrees C for 30 min did not develop any symptoms, and germination was not affected. Thus, hot water treatment at 50 degrees C for 30 min was the best method to eradicate the stunt anthracnose of celery.

    SPRINGER JAPAN KK, 2015年07月, JOURNAL OF GENERAL PLANT PATHOLOGY, 81 (4), 279 - 286, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Analiza Grubanzo Tagle, Izumi Chuma, Yukio Tosa

    A single gene for resistance, designated Rmg7 (Resistance to Magnaporthe grisea 7), was identified in a tetraploid wheat accession, St24 (Triticum dicoccum, KU120), against Br48, a Triticum isolate of Pyricularia oryzae. Two other wheat accessions, Stl7 (T. dicoccum, KU112) and St25 (T dicoccum, KU122), were also resistant against Br48 and showed a similar disease reaction pattern to St24. Crosses between these resistant accessions yielded no susceptible F2 seedlings, suggesting that St24, St17, and St25 carry the same resistance gene. Furthermore, a single avirulence gene corresponding to Rmg7 was detected in a segregation analysis of random Fl progenies between Br48 and MZ5-1-6, an Eleusine isolate virulent to St24 at a higher temperature. This avirulence gene was recognized not only by St24, but also by Stl7 and St25, thus supporting the preceding results indicating that all three accessions carry Rmg7. This resistance gene may have potential in future wheat breeding programs.

    AMER PHYTOPATHOLOGICAL SOC, 2015年04月, PHYTOPATHOLOGY, 105 (4), 495 - 499, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kieu Thi Minh Pham, Hanh Hieu Nguyen, Toshiko Murai, Izumi Chuma, Yukio Tosa, Hitoshi Nakayashiki

    Here we addressed the involvement of histone methylation at H3 lysine 4 (H3K4me) in substrate-dependent activation of cell-wall-degrading enzymes (CWDEs) in Magnaporthe oryzae. Transcriptional activation of 36 putative CWDEs including cellobiohydrolases, endoglucanases, beta-glucosidases, and endoxylanases was assessed by qRT-PCR. Among the CWDE genes, 16 were upregulated during infection and also in media containing their respective substrates with an exception. Interestingly, the substrate-dependent activation of most CWDE genes was severely impaired in a deletion mutant of MoSET1, histone H3K4 methyltransferase in M. oryzae, suggesting that MoSET1 directly or indirectly regulates a wide range of CWDE genes.

    SPRINGER JAPAN KK, 2015年03月, JOURNAL OF GENERAL PLANT PATHOLOGY, 81 (2), 127 - 130, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroki Nakano, Nobuyuki Mizuno, Yukio Tosa, Kentaro Yoshida, Pyoyun Park, Shigeo Takumi

    Hybrid chlorosis, a type of hybrid incompatibility, has frequently been reported in inter-and intraspecific crosses of allopolyploid wheat. In a previous study, we reported some types of growth abnormalities such as hybrid necrosis and observed hybrid chlorosis with mild or severe abnormalities in wheat triploids obtained in crosses between tetraploid wheat cultivar Langdon and four Ae. tauschii accessions and in their derived synthetic hexaploids. However, the molecular mechanisms underlying hybrid chlorosis are not well understood. Here, we compared cytology and gene expression in leaves to characterize the abnormal growth in wheat synthetics showing mild and severe chlorosis. In addition, we compared disease resistance to wheat blast fungus. In total 55 and 105 genes related to carbohydrate metabolism and 53 and 89 genes for defense responses were markedly up-regulated in the mild and severe chlorosis lines, respectively. Abnormal chloroplasts formed in the mesophyll cells before the leaves yellowed in the hybrid chlorosis lines. The plants with mild chlorosis showed increased resistance to wheat blast and powdery mildew fungi, although significant differences only in two, third internode length and maturation time, out of the examined agricultural traits were found between the wild type and plants showing mild chlorosis. These observations suggest that senescence might be accelerated in hybrid chlorosis lines of wheat synthetics. Moreover, in wheat synthetics showing mild chlorosis, the negative effects on biomass can be minimized, and they may show substantial fitness under pathogenpolluted conditions.

    PUBLIC LIBRARY SCIENCE, 2015年03月, PLOS ONE, 10 (3), e0121583, 英語

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    研究論文(学術雑誌)

  • Motoaki Kusaba, Taiga Mochida, Takeshi Naridomi, Yoshikatsu Fujita, Izumi Chuma, Yukio Tosa

    A small and extra chromosome of 1.6 Mb was previously identified in a Pyricularia oryzae strain, 84R-62B. To understand a role of the 1.6 Mb chromosome in the pathogenic changeability of P. oryzae, we performed experiments designed to characterize the 1.6 Mb chromosome in the present study. A gene family encoding secreted protein Pex31s in P. oryzae consists of five homologs, Pex31-A to -E. Among them, Pex31-A and -D are known to be recognized by Pik-m and Pik/Pik-m/Pik-p, respectively. In the present study, we identified Pex31-A and -D in the genome of 84R-62B. Segregation analyses using an F-1 population between 84R-62B and another rice blast strain, Y93-245c-2, revealed a strong linkage between the two homologs and the 1.6 Mb chromosome of 84R-62B. A CHEF-Southern analysis revealed an association between the 1.6 Mb chromosome and the homologs, indicating that both homologs are located on the 1.6 Mb chromosome of 84R-62B. The loss of the 1.6 Mb chromosome was observed in subcultures of a F-1 progeny, F1-327. These subcultures concomitantly acquired virulence on Pik, Pik-m, and Pik-p. The present study is the first report showing that loss of a small and extra chromosome leads to pathogenic mutation of P. oryzae and may provide a new insight into the mechanisms generating pathogenic variation of this fungus.

    SPRINGER, 2014年11月, CURRENT GENETICS, 60 (4), 315 - 325, 英語

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    研究論文(学術雑誌)

  • Christian Joseph R. Cumagun, Vu Lan Anh, Trinh Thi Phuong Vy, Yoshihiro Inoue, Hokuto Asano, Gang-Su Hyon, Izumi Chuma, Yukio Tosa

    In the process (BC3F1 generation) of backcrossing an Avena isolate of Pyricularia oryzae with a Triticum isolate, color mutants with white mycelia were obtained. These white mutants lacked virulence on all (31/31) hexaploid and most (28/32) tetraploid wheat lines tested. In a BC4F1 population, white and black cultures segregated in a 1:1 ratio, suggesting that the mutant phenotype is controlled by a single gene. Furthermore, the mycelial color was perfectly linked with avirulence in the BC4F1 population; white cultures were all avirulent on common wheat (Triticum aestivum) 'Norin 4' (N4) whereas black cultures were all virulent. White cultures in the BC3F1 and BC4F1 generations were also avirulent on tetraploid wheat (T dicoccoides) accession 'KU109' (Tat4), which was susceptible to all cultures derived from the parental wild isolates through the BC2F1 generation. A cross between Tat4 and a susceptible tetraploid (T paleocolchicum) accession 'KU196' (Tat14) produced resistant and susceptible F-2 seedlings in a 3:1 ratio against the white cultures. In the F-3 generation homozygous resistant/segregating/homozygous susceptible lines segregated in a 1:2:1 ratio. These results suggest that the resistance of Tat4 to the white cultures is controlled by a single major gene. This gene, tentatively designated as RmgTd(t), is considered to be a hidden resistance gene because it was not detected with the Br58, F-1, BC1F1, or BC2F1 cultures. Cytological analysis revealed that the moderate resistance controlled by RmgTd(t) was associated with a hypersensitive reaction of mesophyll cells.

    AMER PHYTOPATHOLOGICAL SOC, 2014年06月, PHYTOPATHOLOGY, 104 (6), 634 - 640, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Murata, Takayuki Aoki, Motoaki Kusaba, Yukio Tosa, Izumi Chuma

    In a phylogenetic analysis of species of Magnaporthaceae based on nucleotide sequences of rDNA-ITS and the RPB1 gene, isolates of the tested species were divided into two clusters with high bootstrap support. One group was composed of Pyricularia spp.; the other was composed of Magnaporthe salvinii, M. rhizophila, M. poae, Gaeumannomyces graminis, and G. incrustans. On the basis of this result, we concluded that Pyricularia spp. constitute a large but distinct phylogenetic species group that is not congeneric with Magnaporthe salvinii, the type species of Magnaporthe.

    SPRINGER JAPAN KK, 2014年01月, JOURNAL OF GENERAL PLANT PATHOLOGY, 80 (1), 66 - 72, 英語

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    研究論文(学術雑誌)

  • Trinh Thi Phuong Vy, Gang-Su Hyon, Nguyen Thi Thanh Nga, Yoshihiro Inoue, Izumi Chuma, Yukio Tosa

    Lolium isolate TP2 of Pyricularia oryzae, causal agent of gray leaf spot of perennial ryegrass (Lolium perenne), is virulent on perennial ryegrass, but avirulent on wheat cultivars. Genetic analysis of wheat F-2 populations revealed that the resistance of wheat cultivars Chinese Spring, Shin-chunaga, and Norin 4 to TP2 was conditioned by two genes, R1 and R2. R1 was highly effective, while R2 was less effective. The strong resistance gene R1, designated Rmg6, was mapped on chromosome 1D using microsatellite markers. For revealing genetic mechanisms of avirulence, TP2 was crossed with Triticum isolate Br48. Segregation analysis of their F-1 progenies revealed that the avirulence of TP2 on the three wheat cultivars was conditioned by two unlinked genes, one (A1) highly effective and the other (A2) less effective. These results suggest that the incompatibility between TP2 and the common wheat cultivars is conditioned by two gene pairs; the Rmg6-A1 interaction results in strong resistance, and the R2-A2 interaction results in moderate resistance.

    SPRINGER JAPAN KK, 2014年01月, JOURNAL OF GENERAL PLANT PATHOLOGY, 80 (1), 59 - 65, 英語

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    研究論文(学術雑誌)

  • Ken-ichi Ikeda, Ba Van Vu, Naoki Kadotani, Masaki Tanaka, Toshiki Murata, Kohta Shiina, Izumi Chuma, Yukio Tosa, Hitoshi Nakayashiki

    The long terminal repeat retrotransposon, Magnaporthe gypsy-like element (MAGGY), has been shown to be targeted for cytosine methylation in a subset of Magnaporthe oryzae field isolates. Analysis of the F-1 progeny from a genetic cross between methylation-proficient (Br48) and methylation-deficient (GFSI1-7-2) isolates revealed that methylation of the MAGGY element was governed by a single dominant gene. Positional cloning followed by gene disruption and complementation experiments revealed that the responsible gene was the DNA methyltransferase, MoDMT1, an ortholog of Neurospora crassa Dim-2. A survey of MAGGY methylation in 60 Magnaporthe field isolates revealed that 42 isolates from rice, common millet, wheat, finger millet, and buffelgrass were methylation proficient while 18 isolates from foxtail millet, green bristlegrass, Japanese panicgrass, torpedo grass, Guinea grass, and crabgrass were methylation deficient. Phenotypic analyses showed that MoDMT1 plays no major role in development and pathogenicity of the fungus. Quantitative polymerase chain reaction analysis showed that the average copy number of genomic MAGGY elements was not significantly different between methylation-deficient and -proficient field isolates even though the levels of MAGGY transcript were generally higher in the former group. MoDMT1 gene sequences in the methylation-deficient isolates suggested that at least three independent mutations were responsible for the loss of MoDMT1 function. Overall, our data suggest that MoDMT1 is not essential for the natural life cycle of the fungus and raise the possibility that the genus Magnaporthe may be losing the mechanism of DNA methylation on the evolutionary time scale.

    GENETICS SOC AM, 2013年11月, GENETICS, 195 (3), 845 - +, 英語

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    研究論文(学術雑誌)

  • Yoshihiro Inoue, Ryota Mori, Yujiro Takahashi, So Kiguchi, Takashi Enomoto, Izumi Chuma, Yukio Tosa

    To elucidate genetic mechanisms of host species specificity between graminicolous anthracnose fungi and gramineous plants, infection assays were performed with a Sorghum isolate (Colletotrichum sublineolum), a Digitaria isolate (C. hanaui), a Polypogon isolate (C. cereale), and an Avena isolate (C. cereale). They were specifically virulent on the plants from which they were isolated. When 72 wheat lines were inoculated with an unadapted isolate from Asia Minor bluegrass (Cgp29), however, some exceptional cultivars were recognized. Although most cultivars were resistant to Cgp29, 'Hope' was susceptible. In F-2 populations derived from crosses between three resistant cultivars-'Norin 4' (N4), 'Chinese Spring' (CS), and 'Shin-chunaga' (Sch) and the susceptible Hope, resistant and susceptible seedlings segregated in a 3:1 ratio, suggesting that a major gene is involved in the resistance of each cultivar to Cgp29. In F2 populations derived from crosses between the three resistant cultivars, all seedlings were resistant, suggesting that these three cultivars carry the same gene. This resistance gene was designated as "resistance to Colletotrichum cereale 1" (Rcc1). Analysis with the CS Hope chromosome substitution lines and molecular mapping revealed that Rcc1 was located on the long arm of chromosome 5A. Cytologically, Rcc1 was mainly associated with hypersensitive reaction. These results suggest that major genes similar to those controlling cultivar specificity are involved in the resistance of wheat against the unadapted isolate of C. cereale.

    AMER PHYTOPATHOLOGICAL SOC, 2013年06月, PHYTOPATHOLOGY, 103 (6), 575 - 582, 英語

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    研究論文(学術雑誌)

  • Trinh Xuan Hoat, Hitoshi Nakayashiki, Qian Yang, Yukio Tosa, Shigeyuki Mayama

    Victorin, the host-selective toxin produced by the fungus Cochliobolus victoriae, induces programmed cell death (PCD) in victorin-sensitive oat lines with characteristic features of animal apoptosis, such as mitochondrial permeability transition, chromatin condensation, nuclear DNA laddering and rRNA/mRNA degradation. In this study, we characterized a calcium-binding protein, namely AsALG-2, which might have a role in the victorin-induced PCD. AsALG-2 is homologous to the Apoptosis-Linked Gene ALG-2 identified in mammalian cells. Northern blot analysis revealed that the accumulation of AsALG-2 transcripts increased during victorin-induced PCD, but not during necrotic cell death. Salicylic acid, chitosan and chitin strongly activated the expression of general defence response genes, such as PR-10; however, neither induced cell death nor the accumulation of AsALG-2 mRNA. Pharmacological studies indicated that victorin-induced DNA laddering and AsALG-2 expression were regulated through similar pathways. The calcium channel blocker, nifedipine, moderately inhibited the accumulation of AsALG-2 mRNA during cell death. Trifluoperazine (calmodulin antagonist) and K252a (serine-threonine kinase inhibitor) reduced the victorin-induced phytoalexin accumulation, but did not prevent the victorin-induced DNA laddering or accumulation of AsALG-2 mRNA. Taken together, our investigations suggest that there is a calcium-mediated signalling pathway in animal and plant PCD in common.

    WILEY-BLACKWELL, 2013年04月, MOLECULAR PLANT PATHOLOGY, 14 (3), 222 - 229, 英語

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    研究論文(学術雑誌)

  • Ba Van Vu, Kenji Itoh, Quoc Bao Nguyen, Yukio Tose, Hitoshi Nakayashiki

    Upon infection, phytopathogenic fungi secrete an array of hydrolytic enzymes that can degrade components of the host epidermis, including waxes, the cuticle, and cell walls. Cellulases, which can hydrolyze crystalline cellulose in the plant cell wall, are among these hydrolytic enzymes. Here, we provide RNAi-based evidence to show that cellulases belonging to glycosyl hydrolase (GH) families 6 and 7 contribute to the penetration of the host epidermis and further invasion by the phytopathogenic fungus Magnaporthe oryzae. The GH6 and GH7 cellulases likely include all members of the cellobiohydrolase family and some endoglucanases in M. oryzae. Quantitative reverse-transcriptase polymerase chain reaction analysis indicated that more than half of the cellulases were highly induced during infection. We constructed knock-down (KD) mutants of these cellulases using the building blocks method we reported previously. The transcript levels of the target genes and cellulase activity were considerably reduced in the KD mutants. The KD mutants resulted in fewer lesions, less penetration, and infection of fewer cells compared with the parent strain. Cytological analyses showed that a high rate of papilla formation blocked invasion of the KD mutants into host cells. These results suggest that the GH6 and GH7 cellulases play roles in the virulence of M. oryzae.

    AMER PHYTOPATHOLOGICAL SOC, 2012年09月, MOLECULAR PLANT-MICROBE INTERACTIONS, 25 (9), 1135 - 1141, 英語

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    研究論文(学術雑誌)

  • Nguyen Thi Thanh Nga, Yoshihiro Inoue, Izumi Chuma, Gang-Su Hyon, Kazuma Okada, Trinh Thi Phuong Vy, Motoaki Kusaba, Yukio Tosa

    Barley cultivars show various patterns of resistance against isolates of Magnaporthe oryzae and M. grisea. Genetic mechanisms of the resistance of five representative barley cultivars were examined using a highly susceptible barley cultivar, `Nigrate', as a common parent of genetic crosses. The resistance of the five cultivars against Setaria, Oryza, Eleusine, and Triticum isolates of M. oryzae was all attributed to a single locus, designated as Rino2. Nevertheless, the Rino2 locus in each cultivar was effective against a different range of isolates. Genetic analyses of pathogenicity suggested that each cultivar carries an allele at the Rmo2 locus that recognizes a different range of avirulence genes. One allele, Rmo2.a, corresponded to PWTI, which conditioned the avirulence of Setaria and Oryza isolates on wheat, in a gene-for-gene manner. The other alleles, Rino2.b, Rino2.c, and Rmo2.d, corresponded to more than one avirulence gene. On the other hand, the resistance of those cultivars to another species, M. grisea, was conditioned by another locus, designated as Rmo3. These results suggest that Rmo2 is effective against a broad range of blast isolates but is specific to M. oryzae. Molecular mapping revealed that Rino2 is located on the 7H chromosome.

    AMER PHYTOPATHOLOGICAL SOC, 2012年07月, PHYTOPATHOLOGY, 102 (7), 674 - 682, 英語

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    研究論文(学術雑誌)

  • Gang-Su Hyon, Nguyen Thi Thanh Nga, Izumi Chuma, Yoshihiro Inoue, Hokuto Asano, Nobuaki Murata, Motoaki Kusaba, Yukio Tosa

    The Magnaporthe oryzae-M. grisea species complex is composed of several host-specific subgroups, but does not contain a barley-specific subgroup. To characterize the relationship between barley and these subgroups, we inoculated 24 barley cultivars separately with each of 18 isolates from various hosts. The interactions between these cultivars and isolates included various reactions from nonhost-like immune responses to typical host responses. Evenly closely related isolates of the blast fungi caused such contrasting reactions. The immune responses of barley cultivars against a Setaria isolate, Si-1J, were examined in detail. An infection assay with near-isogenic fungal strains suggested that PWT1, which was first identified as a major gene conditioning the avirulence of Si-1J on wheat, was involved in the avirulence on two-thirds of the barley cultivars. At the cytological level, the immune responses were associated with both papilla formation and hypersensitive reaction (HR). Of these two, however, HR played a more critical role than papilla formation. Studying the interactions of barley with M. oryzae and M. grisea may reveal various steps in the process of host specialization of a parasite species and the concomitant evolution of host resistance.

    SPRINGER TOKYO, 2012年07月, JOURNAL OF GENERAL PLANT PATHOLOGY, 78 (4), 237 - 246, 英語

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    研究論文(学術雑誌)

  • Hiromasa Saitoh, Shizuko Fujisawa, Chikako Mitsuoka, Akiko Ito, Akiko Hirabuchi, Kyoko Ikeda, Hiroki Irieda, Kae Yoshino, Kentaro Yoshida, Hideo Matsumura, Yukio Tosa, Joe Win, Sophien Kamoun, Yoshitaka Takano, Ryohei Terauchi

    To search for virulence effector genes of the rice blast fungus, Magnaporthe oryzae, we carried out a large-scale targeted disruption of genes for 78 putative secreted proteins that are expressed during the early stages of infection of M. oryzae. Disruption of the majority of genes did not affect growth, conidiation, or pathogenicity of M. oryzae. One exception was the gene MC69. The mc69 mutant showed a severe reduction in blast symptoms on rice and barley, indicating the importance of MC69 for pathogenicity of M. oryzae. The mc69 mutant did not exhibit changes in saprophytic growth and conidiation. Microscopic analysis of infection behavior in the mc69 mutant revealed that MC69 is dispensable for appressorium formation. However, mc69 mutant failed to develop invasive hyphae after appressorium formation in rice leaf sheath, indicating a critical role of MC69 in interaction with host plants. MC69 encodes a hypothetical 54 amino acids protein with a signal peptide. Live-cell imaging suggested that fluorescently labeled MC69 was not translocated into rice cytoplasm. Site-directed mutagenesis of two conserved cysteine residues (Cys36 and Cys46) in the mature MC69 impaired function of MC69 without affecting its secretion, suggesting the importance of the disulfide bond in MC69 pathogenicity function. Furthermore, deletion of the MC69 orthologous gene reduced pathogenicity of the cucumber anthracnose fungus Colletotrichum orbiculare on both cucumber and Nicotiana benthamiana leaves. We conclude that MC69 is a secreted pathogenicity protein commonly required for infection of two different plant pathogenic fungi, M. oryzae and C. orbiculare pathogenic on monocot and dicot plants, respectively.

    PUBLIC LIBRARY SCIENCE, 2012年05月, PLOS PATHOGENS, 8 (5), e1002711, 英語

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    研究論文(学術雑誌)

  • Hirofumi Suzuki, Fumihiko Suzuki, Motoaki Kusaba, Yukio Tosa

    In Mie Prefecture in Japan, rice blast isolates resistant to melanin biosynthesis inhibitors targeting scytalone dehydratase (SDH) were first observed in 2005. To analyze the distribution of the resistant isolates, 527 isolates were collected from wide areas in this prefecture during 2006 and 2007. Almost half of the isolates collected (233 of 527 isolates) carried a point mutation in the SDH gene conferring the resistant phenotype. To compare population structures of resistant and sensitive isolates, we analyzed the isolates with repetitive-element-based PCR DNA fingerprinting using a single primer complementary to a sequence in the terminal inverted repeat of transposable element Pot2. A majority of the resistant isolates were classified into a single DNA fingerprint haplotype, Mie 1. Despite its prevalence in the resistant isolates, Mie 1 was not found in the sensitive isolates. Furthermore, in a dendrogram constructed from the DNA fingerprint data, Mie 1 and six other haplotypes formed a cluster composed of resistant isolates alone. These results suggest that the resistant isolates that belonged to the Mie 1 haplotype had migrated from regions outside Mie Prefecture and selectively propagated in a short period in this prefecture.

    SPRINGER TOKYO, 2012年03月, JOURNAL OF GENERAL PLANT PATHOLOGY, 78 (2), 106 - 114, 英語

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    研究論文(学術雑誌)

  • Kohhei Uchihashi, Hitoshi Nakayashiki, Kaoru Okamura, Atsushi Ishihara, Yukio Tosa, Pyoyun Park, Shigeyuki Mayama

    We determined the temporal and spatial localization of the phytoalexin avenanthramide A, and its biosynthetic enzyme, hydroxycinnamoyl-CoA: hydroxyanthranilate N-hydroxy-cinnamoyltransferase (AsHHT) in oat leaves infected with the crown rust fungus. Accumulation of avenanthramide A and AsHHT was first observed predominantly in hypersensitive response (HR) cells at 36 h post inoculation (hpi), and later in adjacent cells at 48 hpi. At 120 hpi, avenanthramide A was detected in a wider area of infected tissues, but AsHHT-positive signals were only observed in the HR and adjacent cells, suggesting that avenanthramide A was synthesized around the HR cells, and then transported to other parts of the infected tissues in a center-to-periphery manner. (C) 2011 Elsevier Ltd. All rights reserved.

    ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2011年12月, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 76 (3-4), 173 - 181, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Izumi Chuma, Yuma Hotta, Yukio Tosa

    Dynamics of chromosomal ends during meiosis was examined using a tetrad F(1) population derived from a cross between Setaria and Triticum isolates of Magnaporthe oryzae. Telomeric fragments were liberated by six restriction enzymes and detected with a telomere probe. Each fragment was assigned to one of the 14 chromosomal ends using chromosome-specific markers. Size shifts and non-Mendelian segregation (4:0, 3:1, 1:3, and 0:4) were frequently observed in these telomeric fragments and were considered to be caused by deletion, insertion, point mutation, and gene conversion. Similar results were obtained in another tetrad F(1) population derived from a cross between Oryza and Triticum isolates. These results suggest that subtelomeric regions are unstable during meiosis and are prone to various rearrangements including gene conversion.

    SPRINGER TOKYO, 2011年11月, JOURNAL OF GENERAL PLANT PATHOLOGY, 77 (6), 317 - 325, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nguyen, Q.B, Itoh, K, Van, V.B, Tosa Yukio, Nakayashiki Hitoshi

    Due to functional redundancy, it is often difficult to genetically analyse the biological function of fungal cell wall-degrading enzymes that belong to a gene family. To overcome this difficulty, we used RNAi to knock-down (KD) multiple xylanase genes to eluci-date their roles in the pathogenicity of the blast fungus, Magnaporthe oryzae. To obtain the maximum average efficiency of gene silencing for the xylanase genes, we used the 'building blocks method', in which a 40 bp sequence was chosen from an endoxylanase gene, and 10 such sequences from 10 endoxylanases were combined to make an artificial RNAi trigger by synthetic DNA. Quantitative RT-PCR analysis revealed that the transcript levels of all the expressed xylanase genes were significantly reduced in KD mutants with the artificial RNAi trigger. Even though the KD mutants did not completely lose their pathogenicity to host plants, the number of lesions, rate of penetration and extent of infected cells were all reduced in KD mutant-infected leaves. The degree of pathogenicity reduction was associated with the silencing levels of xylanase mRNA and enzymatic activity in the KD mutants. Cytological analysis indicated that xylanases play significant roles in both vertical penetration and horizontal expansion of M. oryzae in infected plants.

    WILEY-BLACKWELL, 2011年08月, Mol. Microbiol., 81 (4), 1008 - 1019, 英語

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    研究論文(学術雑誌)

  • Izumi Chuma, Chihiro Isobe, Yuma Hotta, Kana Ibaragi, Natsuru Futamata, Motoaki Kusaba, Kentaro Yoshida, Ryohei Terauchi, Yoshikatsu Fujita, Hitoshi Nakayashiki, Barbara Valent, Yukio Tosa

    Magnaporthe oryzae is the causal agent of rice blast disease, a devastating problem worldwide. This fungus has caused breakdown of resistance conferred by newly developed commercial cultivars. To address how the rice blast fungus adapts itself to new resistance genes so quickly, we examined chromosomal locations of AVR-Pita, a subtelomeric gene family corresponding to the Pita resistance gene, in various isolates of M. oryzae (including wheat and millet pathogens) and its related species. We found that AVR-Pita (AVR-Pita1 and AVR-Pita2) is highly variable in its genome location, occurring in chromosomes 1, 3, 4, 5, 6, 7, and supernumerary chromosomes, particularly in rice-infecting isolates. When expressed in M. oryzae, most of the AVR-Pita homologs could elicit Pita-mediated resistance, even those from non-rice isolates. AVR-Pita was flanked by a retrotransposon, which presumably contributed to its multiple translocation across the genome. On the other hand, family member AVR-Pita3, which lacks avirulence activity, was stably located on chromosome 7 in a vast majority of isolates. These results suggest that the diversification in genome location of AVR-Pita in the rice isolates is a consequence of recognition by Pita in rice. We propose a model that the multiple translocation of AVR-Pita may be associated with its frequent loss and recovery mediated by its transfer among individuals in asexual populations. This model implies that the high mobility of AVR-Pita is a key mechanism accounting for the rapid adaptation toward Pita. Dynamic adaptation of some fungal plant pathogens may be achieved by deletion and recovery of avirulence genes using a population as a unit of adaptation.

    PUBLIC LIBRARY SCIENCE, 2011年07月, PLOS PATHOGENS, 7 (7), e1002147, 英語

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    研究論文(学術雑誌)

  • Ryohei Terauchi, Kentaro Yoshida, Hiromasa Saitoh, Hiroyuki Kanzaki, Yudai Okuyama, Koki Fujisaki, Ayako Miya, Akira Abe, Muluneh Tamiru, Yukio Tosa

    Rice blast caused by an ascomycete fungus Magnaporthe oryzae is the most devastating disease of rice worldwide. Thus understanding of the molecular mechanisms of Magnaporthe-rice interactions is crucial to devise efficient means of disease control. The availability of whole genome sequences of both M. oryzae and rice enables us to analyze their DNA polymorphisms on the genomic scale for the purpose of association genetics as well as for population genomics analysis. Studies on the association between phenotypes-DNA polymorphisms allowed us to isolate three M. oryzae AVR genes, AVR-Pia, AVR-Pii, AVR-Pik/km/kp as well as a rice R-gene, Pia. Population genomics addresses the patterns of DNA polymorphisms to identify the genomic regions under natural selection, a potentially useful tool to isolate the genes involved in plant-pathogen interactions. Recent progress in next-generation sequencing technologies predicts that these approaches would be commonly used to elucidate various host-pathogen interactions.

    SPRINGER, 2011年, AUSTRALASIAN PLANT PATHOLOGY, 40 (4), 328 - 334, 英語

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    研究論文(学術雑誌)

  • Tetsu Tsurushima, Yukari Minami, Hisashi Miyagawa, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama

    The ethyl acetate extract of the conidia germination fluid from an Avena isolate (Br58) of Pyricularia oryzae had chlorosis-inducing activity on oat leaf segments. The same activity was also present in the acetone extract of an oatmeal agar culture of Br58. Fungal cultures were used for a large-scale preparation. A series of acetone and ethyl acetate extraction monitored by chromatography was used to isolate an active fraction. The active principle was purified by HPLC. We show by NAIR and LC/MS that the toxin was an oxidized C18 unsaturated fatty acid named Mag-toxin. Mag-toxin induced chlorosis on oat leaf segments incubated in the light but not in the dark. Reactive oxygen species (ROS) and cell death were induced by Mag-toxin in oat cells. The subcellular localization of ROS generation induced by the toxin treatment was correlated with the location of mitochondria. Interestingly, the induction of ROS generation and cell death by Mag-toxin was light-independent.

    TAYLOR & FRANCIS LTD, 2010年11月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74 (11), 2220 - 2225, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masaki Tanaka, Gang-Su Hyon, Toshiki Murata, Hitoshi Nakayashiki, Yukio Tosa

    Eleusine isolates (members of the Eleusine subgroup) of Pyricularia oryzae are divided into two groups, Ec-I and Ec-II, differentiated by molecular markers. A multilocus phylogenetic analysis and DNA fingerprinting suggested that Ec-I isolates are very close to Eragrostis isolates rather than Ec-II isolates. Infection assays revealed that Ec-II and Eragrostis isolates were exclusively virulent on finger millet and weeping lovegrass, respectively, whereas Ec-I isolates were virulent on both. The avirulence or virulence on weeping lovegrass perfectly corresponded to the presence or absence of an avirulence gene, PWL1; all Ec-II isolates carried an identical, functional PWL1, whereas none of Ec-I isolates or Eragrostis isolates carried it. A comparison of PWL1 flanking regions revealed that Ec-II isolates had a peculiar structure produced by an insertion (or translocation) of a DNA fragment carrying PWL1. Based on these results, a model was constructed which illustrated possible pathways to the establishment of the Eleusine subgroup.

    AMER PHYTOPATHOLOGICAL SOC, 2010年06月, MOLECULAR PLANT-MICROBE INTERACTIONS, 23 (6), 771 - 783, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Izumi Chuma, Su-Wen Zhan, Shunsuke Asano, Nguyen Thi Thanh Nga, Trinh Thi Phuong Vy, Michiko Shirai, Kana Ibaragi, Yukio Tosa

    The pathogenicity to wheat (Pwt1) locus conditions host species specificity of Magnaporthe oryzae on wheat. GFSI 1-7-2 (Setaria isolate) carries the avirulence allele (PWT1) at this locus while Br48 (Triticum isolate) carries the virulence allele (pwt1). An F(1) culture derived from a cross between GFSI 1-7-2 and Br48 was backcrossed with Br48 to produce a tester population in which PWT1 alone segregated. When hexaploid wheat lines were inoculated with the BC(1)F(1) testers, they were all resistant to all PWT1 carriers and susceptible to all mill carriers, suggesting that they recognize PWT1. When barley cultivars were inoculated with the testers, they showed the same pattern of reactions as the hexaploid lines, suggesting that the barley cultivars also recognize PWT1. These results suggest that PWT1 is a fundamental gene that universally conditions the avirulence of Setaria isolates on two staple crops, hexaploid wheat and barley. Interestingly, tetraploid wheat lines did not recognize PWT1. Molecular mapping using the F(1) and BC(1)F(1) populations revealed that the Pwt1 locus is located on chromosome 2 and tightly linked to the ribosomal DNA locus and a telomere.

    AMER PHYTOPATHOLOGICAL SOC, 2010年05月, PHYTOPATHOLOGY, 100 (5), 436 - 443, 英語

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    研究論文(学術雑誌)

  • Hiromasa Saitoh, Shizuko Fujisawa, Akiko Ito, Chikako Mitsuoka, Thomas Berberich, Yukio Tosa, Makoto Asakura, Yoshitaka Takano, Ryohei Terauchi

    SPM1, encoding a putative subtilisin-like protease, is involved in pathogenicity of the rice blast fungus Magnaporthe oryzae, but its detailed function remains unknown. Here, we report that SPM1 encodes a vacuole-localized protease that is a critical component for autophagy during the infection process of M. oryzae. Detailed phenotypic analysis of targeted disruption mutants of SPM1 revealed that the mutants have pleiotropic defects in infection-related steps including germination, appressorium formation, host invasion and postinvasive growth, indicating the requirement of Spm1 function for the broad phase of infection. It has been shown that the Spm1 homolog of yeast functions in autophagy, the degradation machinery mediated by vacuoles, implying the involvement of Spm1 in autophagy in M. oryzae. In-gel protease activity assay of the recombinant Spm1 protein indicated that Spm1 had a protease activity. An Spm1-GFP fusion protein was detected inside vacuoles of fungal cells, indicating that Spm1 is a protease localized in vacuoles. Furthermore, degradation of putative autophagic bodies was retarded in vacuoles of the spm1 mutant. These data strongly suggest that SPM1-encoded protease functions in autophagy required for the pathogenicity of M. oryzae.

    OXFORD UNIV PRESS, 2009年11月, FEMS MICROBIOLOGY LETTERS, 300 (1), 115 - 121, 英語

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    研究論文(学術雑誌)

  • Izumi Chuma, Takeshi Shinogi, Naoki Hosogi, Ken-ichi Ikeda, Hitoshi Nakayashiki, Pyoyun Park, Yukio Tosa

    The inner cellular structure of microconidia of Magnaporthe oryzae was examined using fluorescent probes and electron microscopic techniques. The volume of the nucleus relative to the cell was significantly larger in microconidia than in macroconidia or vegetative hyphae, similar to observations for spermatia of other fungi. Selective fluorescent staining revealed that cytosolic RNA was less abundant in microconidia than in macroconidia and germ tubes, suggesting that general metabolic activity in microconidia is low. Consistently, GFP expression driven by the TrpC promoter was highly active during the formation of phialides and microconidia but gradually decreased as the microconidia matured. Such data suggest that microconidia are in a quiescent or dormant state.

    SPRINGER TOKYO, 2009年10月, JOURNAL OF GENERAL PLANT PATHOLOGY, 75 (5), 353 - 358, 英語

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    研究論文(学術雑誌)

  • N. T. T. Nga, V. T. B. Hau, Y. Tosa

    Common wheat cultivars are resistant to Magnaporthe grisea. a crabgrass (Digitaria sangumalis)-specific species of the blast fungus To dissect the genetic basis of this "nonhost" type of resistance. we need,in exceptional cultivar that is susceptible to M. grisea A screening under various conditions revealed that Triticum aestivum 'Chinese Spring' (CS) was susceptible to M grisea isolate Dig41 when incubated at high temperature (26 degrees C) after inoculation By contrast, T aestivum 'P168'. 'Shin-chunaga' (Sch), 'Norin 4' (M). 'Norin 26' (N26), 'Norin 29' (N29), 'Red Egyptian' (RE), and 'Salmon' (Slm) and Triticum compactum 'No. 44' (Cmp) were highly resistant even at the high temperature. When F(2) seedlings derived from crosses between the resistant cultivars and CS were inoculated with Dig41, they segregated in a 3.1 ratio of resistant 10 Susceptible, Suggesting that the resistance of each cultivar is controlled by one major gene. Crosses of N4 with P168, Sch. N26. N29. and Cmp yielded no susceptible F2 seedlings. suggesting that these six cultivars share the same gene Similarly, I cross between RE and Slm yielded no susceptible F2 seedlings, suggesting that these two cultivars share the same gene On the other hand, crosses between the N4 group and the RE group produced resistant and susceptible seedlings in a 15.1 ratio. indicating that these two groups carry different genes inherited independently. The gene in N4 wits located on chromosome 4A by monosomic analysis and designated Rmg4. while the gene in RE was located on chromosome 6D using a series of chromosome substitution lines and designated Rmg5. These results suggest that the resistance of common wheat to M grisea, an inappropriate species of the blast fungus, is under a simple genetic control

    NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS, 2009年09月, GENOME, 52 (9), 801 - 809, 英語

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    研究論文(学術雑誌)

  • Masaki Tanaka, Hitoshi Nakayashiki, Yukio Tosa

    Population structure of Eleusine isolates of Pyricularia oryzae (Magnaporthe oryzae) was examined using DNA markers. On the basis of rDNA sequences, Eleusine isolates were divided into two groups. One group clustered with Triticum isolates, while the other clustered with Eragrostis isolates. This grouping was supported by DNA fingerprinting with three repetitive elements: MGR586, MGR583, and grasshopper. These results suggest that the population of Eleusine isolates is composed of at least two groups that evolved independently from the original population of P. oryzae. Most of the isolates that were collected just after an outbreak of finger millet blast in the 1970s had almost identical fingerprint profiles although they were collected in distant prefectures. This result supports the idea that the outbreak was caused by seed transmission of a particular strain of Eleusine isolates.

    SPRINGER TOKYO, 2009年06月, JOURNAL OF GENERAL PLANT PATHOLOGY, 75 (3), 173 - 180, 英語

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    研究論文(学術雑誌)

  • Kentaro Yoshida, Hiromasa Saitoh, Shizuko Fujisawa, Hiroyuki Kanzaki, Hideo Matsumura, Kakoto Yoshida, Yukio Tosa, Izumi Chuma, Yoshitaka Takano, Joe Win, Sophien Kamoun, Ryohei Terauchi

    To subvert rice (Oryza sativa) host defenses, the devastating ascomycete fungus pathogen Magnaporthe oryzae produces a battery of effector molecules, including some with avirulence (AVR) activity, which are recognized by host resistance (R) proteins resulting in rapid and effective activation of innate immunity. To isolate novel avirulence genes from M. oryzae, we examined DNA polymorphisms of secreted protein genes predicted from the genome sequence of isolate 70-15 and looked for an association with AVR activity. This large-scale study found significantly more presence/absence polymorphisms than nucleotide polymorphisms among 1032 putative secreted protein genes. Nucleotide diversity of M. oryzae among 46 isolates of a worldwide collection was extremely low (theta = 8.2 x 10(-5)), suggestive of recent pathogen dispersal. However, no association between DNA polymorphism and AVR was identified. Therefore, we used genome resequencing of Ina168, an M. oryzae isolate that contains nine AVR genes. Remarkably, a total of 1.68 Mb regions, comprising 316 candidate effector genes, were present in Ina168 but absent in the assembled sequence of isolate 70-15. Association analyses of these 316 genes revealed three novel AVR genes, AVR-Pia, AVR-Pii, and AVR-Pik/km/kp, corresponding to five previously known AVR genes, whose products are recognized inside rice cells possessing the cognate R genes. AVR-Pia and AVR-Pii have evolved by gene gain/loss processes, whereas AVR-Pik/km/kp has evolved by nucleotide substitutions and gene gain/loss.

    AMER SOC PLANT BIOLOGISTS, 2009年05月, PLANT CELL, 21 (5), 1573 - 1591, 英語

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    研究論文(学術雑誌)

  • Rice blast association genetics reveals three novel avirulence genes, AVR-Pia, AVR-Pii, AVR-Pik/km/kp, whose products are recognized inside rice cells.

    Yoshida, K, Saitoh, H, Fujisawa, S, Kanzaki, H, Matsumura,H, Yoshida, K, Tosa, Y, Chuma, I, Takano, Y, Win, J, Kamoun, S, Terauchi, R

    2009年, Plant Cell, 21, 1573-1591

  • Ryohei Terauchi, Joe Win, Sophien Kamoun, Hideo Matsumura, Hiromasa Saitoh, Hiroyuki Kanzaki, Kentaro Yoshida, Matt Shenton, Thomas Berberich, Shizuko Fujisawa, Akiko Ito, Yoshitaka Takano, Yukio Tosa

    In 2005, a draft sequence of Magnaporthe oryzae isolate 70-15 genome was published (Dean et al. 2005), Complete rice genome sequence was also published in the same year (International Rice Genome Sequencing Project, 2005). As a result, study of the Magnaporthe-rice interaction has entered the "post-genomics" era. The challenge now is how to make use of this large amount of information to improve our understanding of this interaction. By employing genomics information, we are trying to identify and characterize Magnaporthe effectors directly involved in molecular interactions between the pathogen and the host. In this paper, we present a short review of phytopathogen effectors and Magnaporthe avirulence factors. We then briefly describe our current approach to identify new effectors from Magnaporthe.

    SPRINGER, 2009年, ADVANCES IN GENETICS, GENOMICS AND CONTROL OF RICE BLAST DISEASE, 105 - +, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Quoc Bao Nguyen, Naoki Kadotani, Seiji Kasahara, Yukio Tosa, Shigeyuki Mayama, Hitoshi Nakayashiki

    We developed an RNA-silencing vector, pSilent-Dual1 (pSD1), with a convergent dual promoter system that provides a high-throughput platform for functional genomics research in filamentous fungi. In the pSD1 system, the target gene was designed to be transcribed as a chimeric RNA with enhanced green fluorescent protein (eGFP) RNA. This enabled us to efficiently screen the resulting transformants using GFP fluorescence as an indicator of gene silencing. A model study with the eGFP gene showed that pSD1-based vectors induced gene silencing via the RNAi pathway with slightly lower efficiency than did hairpin eGFP RNA-expressing vectors. To demonstrate the applicability of the pSD1 system for elucidating gene function in the rice-blast fungus Magnaporthe oryzae, 37 calcium signalling-related genes that include almost all known calcium-signalling proteins in the genome were targeted for gene silencing by the vector. Phenotypic analyses of the silenced transformants showed that at least 26, 35 and 15 of the 37 genes examined were involved in hyphal growth, sporulation and pathogenicity, respectively, in M. oryzae. These included several novel findings such as that Pmc1-, Spf1- and Neo1-like Ca2+ pumps, calreticulin and calpactin heavy chain were essential for fungal pathogenicity.

    WILEY, 2008年06月, MOLECULAR MICROBIOLOGY, 68 (6), 1348 - 1365, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Toshiki Murata, Naoki Kadotani, Miki Yamaguchi, Yukio Tosa, Shigeyuki Mayama, Hitoshi Nakayashiki

    The LTR-retrotransposon MAGGY was introduced into naive genomes of Magnaporthe oryzae with different genetic backgrounds (wild-type, and MoDcl1 [mdl1] and MoDcl2 [mdl2] dicer mutants). The MoDcl2 mutants deficient in MAGGY siRNA biogenesis generally showed greater MAGGY mRNA accumulation and more rapid increase in MAGGY copy number than did the wild-type and MoDcl1 mutants exhibiting normal MAGGY siRNA accumulation, indicating that RNA silencing functioned as an effective defense against the invading element. Interestingly, however, regardless of genetic background, the rate of MAGGY transposition drastically decreased as its copy number in the genome increased. Notably, in the MoDcl2 mutant, copy-number-dependent MAGGY suppression occurred without a reduction in its mRNA accumulation, and therefore by a silencing mechanism distinct from both transcriptional gene silencing and siRNA-mediated RNA silencing. This might imply that some mechanism possibly similar to post-transcriptional cosuppression of Ty1 retrotransposition in Saccharomyces cerevisiae, which operates regardless of the abundance of target transcript and independent of RNA silencing, would also function in M. oryzae that possesses the RNA silencing machinery.

    OXFORD UNIV PRESS, 2007年09月, NUCLEIC ACIDS RESEARCH, 35 (18), 5987 - 5994, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kenji Hirata, Motoaki Kusaba, Izumi Chuma, Jun Osue, Hitoshi Nakayashiki, Shigeyuki Mayama, Yukio Tosa

    Pyricularia isolates from various host plants were subjected to a multilocus phylogenetic analysis based on rDNA-ITS, actin, beta-tubulin, and calmodulin loci. A combined gene tree resolved seven groups with 100% BS support, suggesting that they are monophyletic groups supported concordantly by all four loci. By incorporating biological and morphological species criteria, each of the seven groups was considered to be a current species. However, phylogenetic relationships among these species were unresolved in the single-gene trees and in the combined tree. Furthermore, the transition from concordance to conflict occurred more than once in the combined gene tree. They were interpreted by assuming that Pyricularia has evolved through repeated species radiation. The transition point other than the current species limit was considered to be the limit of the former species. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2007年07月, MYCOLOGICAL RESEARCH, 111 (Pt 7), 799 - 808, 英語

    [査読有り]

    研究論文(学術雑誌)

  • TX Hoat, H Nakayashiki, Y Tosa, S Mayama

    Here we report that rRNA and mRNA are specifically degraded in oat (Avena sativa L.) cells during apoptotic cell death induced by victorin, a host-selective toxin produced by Cochliobolus victoriae. Northern analysis indicated that rRNA species from the cytosol, mitochondria and chloroplasts were all degraded via specific degradation intermediates during victorin-induced apoptotic cell death but, in contrast, they were randomly digested in necrotic cell death induced by 30 MM CuSO4 and heat shock. This indicates that specific rRNA cleavage could be controlled by an intrinsic program. We also observed specific cleavage of mRNA of housekeeping genes such as actin and ubiquitin during victorin-induced cell death. Interestingly, no victorin-induced mRNA degradation was detected with stress-responding genes such as PR-1, PR-10 and GPx throughout the experimental period. The RNA degradation mostly, but not always, occurred in parallel with DNA laddering, but pharmacological studies indicated that these processes are regulated by different signaling pathways with some overlapping upstream signals.

    BLACKWELL PUBLISHING, 2006年06月, PLANT JOURNAL, 46 (6), 922 - 933, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y Tosa, H Tamba, K Tanaka, S Mayama

    A Triticum isolate (pathogenic on wheat) of Magnaporthe oryzae was crossed with an Oryza isolate (pathogenic on rice) to elucidate mechanisms of their parasitic specificity on wheat. When the pathogenicity of their F, Cultures (hybrids between a Triticum isolate and an Oryza isolate) was tested on wheat, avirulent and virulent Cultures segregated in a 7:1 ratio. This result suggests that three loci are involved in avirulence of the Oryza isolate on wheat. One of the three loci conditioned papilla formation, whereas the others conditioned the hypersensitive reaction. Allelism tests revealed that the locus conditioning papilla formation is Pwt2 while one of the two loci conditioning the hypersensitive reaction is Pwt1. The other locus conditioning the hypersensitive reaction was different from any other known loci and. therefore, was designated as Pwt5.

    AMER PHYTOPATHOLOGICAL SOC, 2006年05月, PHYTOPATHOLOGY, 96 (5), 480 - 484, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A Kiba, Y Sangawa, K Ohnishi, N Yao, P Park, H Nakayashiki, Y Tosa, S Mayama, Y Hikichi

    Pseudomonas cichorii is the major causal agent of bacterial rot of lettuce. Collapse and browning symptoms were observed in lettuce leaf tissue from 15 to 24 h after inoculation (HAI) with P cichorii; superoxide anion generation was detected at I to 6 HAI; and cell death was induced at 6 HAI, reaching a maximum at approximately 9 and 12 HAI. Heterochromatin condensation and DNA laddering also were observed within 3 HAI. Pharmacological studies showed that induction of cell death and DNA laddering was closely associated with de novo protein synthesis, protein kinase, intracellular reactive oxygen species, DNase, serine protease, and caspase III-like protease. Moreover, chemicals, which inhibited the induction of cell death and DNA laddering, also suppressed the development of disease symptoms. These results suggest that apoptotic cell death might be closely associated with the development of bacterial rot caused by P cichorii.

    AMER PHYTOPATHOLOGICAL SOC, 2006年02月, MOLECULAR PLANT-MICROBE INTERACTIONS, 19 (2), 112 - 122, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masaru Sakamoto, Yasuomi Tada, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama

    Reactive oxygen species (ROS) are thought to be involved in various forms of programmed cell death (PCD) in animal and plant cells. PCD, along with the production of ROS, occurs during plant-pathogen interactions. Here we show that victorin, a host-specific toxin produced by Cochliobolus victoriae, which causes victoria blight of oats, induces two phases of intracellular ROS production in victorin-sensitive oat mesophyll cells. The initial production of ROS is restricted at mitochondria and not accompanied with cellular oxidative damage. Later production of ROS is dispersed into cells concomitant with lipid peroxidation, chloroplast dysfunction, and cell death. Superoxide dismutase can clearly suppress the initial ROS production and delay the progression of cell death. These data indicate that the initial ROS production may be involved in the cell death induction process, and the later ROS production may play important roles in events leading to cellular disruption. © The Phytopathological Society of Japan and Springer-Verlag 2005.

    2005年12月, Journal of General Plant Pathology, 71 (6), 387 - 394, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y Tada, Y Ohura, S Betsuyaku, T Shinogi, M Sakamoto, Y Ohura, S Hata, T Mori, Y Tosa, S Mayama

    The host-selective toxin victorin is produced by Cochliobolus victoriae, the causal agent of victoria blight of oats. Victorin has been shown to bind to the P protein of the glycine decarboxylase complex (GDC) in mitochondria, and induce defense-related responses such as phytoalexin synthesis, extracellular alkalization and programmed cell death. However, evidence demonstrating that the GDC plays a critical role in the onset of cell death is still lacking, and the role of defense-like responses in the pathogenicity has yet to be elucidated. Here, cytofluorimetric analyses, using the fluorescein (VicFluor) or bovine serum albumin-fluorescein derivative of victorin (VicBSA), demonstrated that victorin-induced cell death occurs before these conjugates traverse the plasma membrane. As with native victorin, VicBSA clearly elicits apoptosis-like cell death, production of phytoalexin, extracellular alkalization, and generation of nitric oxide and reactive oxygen intermediates. These results suggest that the initial recognition of victorin takes place on the cell surface, not in mitochondria, and leads to the activation of a battery of victorin-induced responses. Pharmacological studies showed that extracellular alkalization is the essential regulator for both victorin- and VicBSA-induced cellular responses. We propose a model where victorin may kill the host cell by activating an HR-like response, independent of the binding to the GDC, through ion fluxes across the plasma membrane.

    OXFORD UNIV PRESS, 2005年11月, PLANT AND CELL PHYSIOLOGY, 46 (11), 1787 - 1798, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T Tsurushima, LD Don, K Kawashima, J Murakami, H Nakayashiki, Y Tosa, S Mayama

    Culture filtrates from 72 isolates of Pyricularia, grouped into 13 rDNA types, were analysed via HPLC. Of these isolates, 31 (r9 DNA type) from crabgrass (Digitaria sanguinalis), one (r9 DNA type) from pangolagrass (Digitaria smutsii) and six (r8 DNA type) from Digitaria horizontalis produced 20-280 mu g pyrichalasin H per millilitre of culture. These same isolates were pathogenic on five Digitaria species. Interestingly, two isolates, KM-1 and Br 29, which were originally isolated from Digitaria plants, did not produce pyrichalasin H, nor caused blast lesion on Digitaria plants. Because these two isolates were identified as Digitaria pathogens by PCR analysis using Digitaria-specific primers, they are likely to be mutants lacking pyrichalasin H production. Isolates that belonged to the remaining 11 rDNA types did not produce pyrichalasin H and were avirulent to Digitaria plants. Therefore, the virulence of Pyricularia on Digitaria plants correlates with pyrichalasin H production. Pyrichalasin H was also present in spore germination fluid of a crabgrass isolate (IBDS 5-1-1), but not in that of isolates from rice, foxtail millet, finger millet, common millet and wheat. In addition, pyrichalasin H was detected in host leaves infected with IBDS 5-1-1, but not in leaves from other plants infected with compatible Pyricularia isolates. Pretreatment of leaf sheaths of crabgrass with 3 mu g/mL pyrichalasin H led to the penetration and colonization by non-host isolates. Overall, these results indicate that production of pyrichalasin H is responsible for the genus-specific pathogenicity of Digitaria isolates.

    WILEY-BLACKWELL, 2005年11月, MOLECULAR PLANT PATHOLOGY, 6 (6), 605 - 613, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y Tosa, J Osue, Y Eto, HS Oh, H Nakayashiki, S Mayama, SA Leong

    The significance of AVR1-CO39, an avirulence gene of the blast fungus corresponding to Pi-CO39(t) in rice cultivars, during the evolution and differentiation of the blast fungus was evaluated by studying its function and distribution in Pyricularia spp. When the presence or absence of AVR1-C039 was plotted on a dendrogram constructed from ribosomal DNA sequences, a perfect parallelism was observed between its distribution and the phylogeny of Pyricularia isolates. AVR1-CO39 homologs were exclusively present in one species, Pyricularia oryzae, suggesting that AVR1-CO39 appeared during the early stage of evolution of P. oryzae. Transformation assays showed that all the cloned homologs tested are functional as an avirulence gene, indicating that selection has maintained their function. Nevertheless, Oryza isolates (isolates virulent on Oryza spp.) in P oryzae were exceptionally noncarriers of AVR1-CO39. All Oryza isolates suffered from one of the two types of known rearrangements at the Avr1-CO39 locus (i.e., G type and J type). These types were congruous to the two major lineages of Oryza isolates from Japan determined by MGR586 and MAGGY. These results indicate that AVR1-CO39 was lost during the early stage of evolution of the Oryza-specific subgroup of P oryzae. Interestingly, its corresponding resistance gene, Pi-CO39(t), is not widely distributed in Oryza spp.

    AMER PHYTOPATHOLOGICAL SOC, 2005年11月, MOLECULAR PLANT-MICROBE INTERACTIONS, 18 (11), 1148 - 1160, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kenji Hirata, Yukio Tosa, Hitoshi Nakayashiki, Shigeyuki Mayama

    We examined whether PWT4, an avirulence gene of Avena isolates of Magnaporthe oryzae toward wheat, corresponded to Rwt4, a resistance gene identified in wheat cultivar Norin 4, in a one-to-one manner. Twelve wheat cultivars were inoculated with 65X1, an F1 culture with PWT4 derived from a cross between an Avena isolate (Br58) and a Triticum isolate (Br48). Three wheat cultivars (Norin 26, Shin-chunaga, Cheyenne) were resistant and therefore selected as possible carriers of Rwt4. The three cultivars were then inoculated with a population derived from a backcross of 61M2 carrying PWT4 with Br48 carrying pwt4. Segregation analyses revealed that PWT4 operates against the three cultivars. If PWT4 corresponds to Rwt4 in a one-to-one manner, all three cultivars should carry Rwt4. To test if this is the case, the three cultivars were crossed with Chinese Spring (a noncarrier of Rwt4) and Norin 4. When F2 seedlings from Chinese Spring × Norin 26, Chinese Spring × Shin-chunaga, and Chinese Spring × Cheyenne were inoculated with 61M2, resistant and susceptible seedlings segregated in a 3:1 ratio. On the other hand, crosses between the three cultivars and Norin 4 yielded no susceptible F2 seedlings. These results indicate that all three cultivars carry Rwt4. Considering all results, we concluded that PWT4 corresponds to Rwt4 in a one-to-one manner. An inoculation test with Chinese Spring-Cheyenne chromosome substitution lines indicated that Rwt4 is located on chromosome 1D. © The Phytopathological Society of Japan and Springer-Verlag 2005.

    2005年10月, Journal of General Plant Pathology, 71 (5), 340 - 344, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T Shinogi, Y Hamanishi, Y Otsu, YQQ Wang, T Nonomura, Y Matsuda, H Toyoda, Y Narusaka, Y Tosa, S Mayama

    The process of host/non-host determination was dissected in interactions of Epilachna vigintioctopunctata, a specialist herbivore of solanaceous plants, with various plant species. On host plants (tomato and egg plant) the ladybird beetle started feeding within 5 min. On red pepper, another solanaceous plant, it also started feeding within 5 min, but did not continue the feeding as vigorously as on tomato or eggplant. This result suggests that the ladybird beetle recognizes red pepper as a host plant but does not overcome its constitutive resistance. On Chinese cabbage, the ladybird beetle did not start feeding as quickly as on the host plants, but once started, it continued feeding as vigorously as on the host plants. This result suggests that the ladybird beetle does not recognize Chinese cabbage as a host plant but overcomes its constitutive resistance. Subsequently, the effect of induced resistance in a host (tomato) and non-hosts (Chinese cabbage and Arabidopsis) was evaluated. The treatment with methyl jasmonate (MeJA) showed no effects in tomato but decreased the damaged area in Chinese cabbage and Arabidopsis. A feeding test with Arabialopsis mutants supported the idea that induced resistance via the jasmonic acid (JA) pathway is effective against the ladybird beetle on the cruciferous plants. We suggest that a specialist herbivore has to overcome not only constitutive resistance but also induced resistance to utilize the non-host plant as a host, and that induced resistance is one of the factors that determine host specificity of the specialist. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

    ELSEVIER IRELAND LTD, 2005年06月, PLANT SCIENCE, 168 (6), 1477 - 1485, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YQ Wang, Y Ohara, H Nakayashiki, Y Tosa, S Mayama

    Pseudomonas fluorescens FPT9601-T5 was originally identified as an endophytic plant growth-promoting rhizobacteria (PGPR) on tomato. To perform a molecular dissecttion of physiological and biochemical changes occurring in the host triggered by P fluorescens FPT9601-T5 colonization, the model plant Arabidopsis was used in this study. Root colonization of Arabidopsis with P. fluorescens FPT9601-T5 promoted plant growth later than three weeks after inoculation and partially suppressed disease symptoms caused by Pseudomonas syringae pv. tomato DC3000, indicating that R fluorescens FPT9601-T5 acted as a PGPR on Arabidopsis. To obtain a global view on transcript modification during the Arabidopsis-FPT9601-T5 interaction, we performed microarray analysis using Affymetrix Genechip probe arrays representing approximately 22,800 genes. The results showed that 95 and 105 genes were up- or down-regulated, respectively, more than twofold in FPT9601-T5-treated Arabidopsis plants as compared with control plants. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Noteworthy, upon FPT9601-T5 colonization, putative auxin-regulated genes and nodulin-like genes were up-regulated, and some ethylene-responsive genes were down-regulated. Our results suggest that P. fluorescens FPT9601-T5 triggered plant responses in a manner similar to known PGPR and, at least in some aspects, to rhizobia.

    AMER PHYTOPATHOLOGICAL SOC, 2005年05月, MOLECULAR PLANT-MICROBE INTERACTIONS, 18 (5), 385 - 396, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YQ Wang, Y Ohara, H Nakayashiki, Y Tosa, S Mayama

    Pseudomonas fluorescens FPT9601-T5 was originally identified as an endophytic plant growth-promoting rhizobacteria (PGPR) on tomato. To perform a molecular dissecttion of physiological and biochemical changes occurring in the host triggered by P fluorescens FPT9601-T5 colonization, the model plant Arabidopsis was used in this study. Root colonization of Arabidopsis with P. fluorescens FPT9601-T5 promoted plant growth later than three weeks after inoculation and partially suppressed disease symptoms caused by Pseudomonas syringae pv. tomato DC3000, indicating that R fluorescens FPT9601-T5 acted as a PGPR on Arabidopsis. To obtain a global view on transcript modification during the Arabidopsis-FPT9601-T5 interaction, we performed microarray analysis using Affymetrix Genechip probe arrays representing approximately 22,800 genes. The results showed that 95 and 105 genes were up- or down-regulated, respectively, more than twofold in FPT9601-T5-treated Arabidopsis plants as compared with control plants. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Noteworthy, upon FPT9601-T5 colonization, putative auxin-regulated genes and nodulin-like genes were up-regulated, and some ethylene-responsive genes were down-regulated. Our results suggest that P. fluorescens FPT9601-T5 triggered plant responses in a manner similar to known PGPR and, at least in some aspects, to rhizobia.

    AMER PHYTOPATHOLOGICAL SOC, 2005年05月, MOLECULAR PLANT-MICROBE INTERACTIONS, 18 (5), 385 - 396, 英語

    研究論文(学術雑誌)

  • H Nakayashiki, S Hanada, NB Quoc, N Kadotani, Y Tosa, S Mayama

    We have developed a pHANNIBAL-like silencing vector, pSilent-1, for ascomycete fungi, which carries a hygromycin resistance cassette and a transcriptional unit for hairpin RNA expression with a spacer of a cutinase gene intron from the rice blast fungus Magnaporthe oryzae. In M. oryzae, a silencing vector with the cutinase intron spacer (147 bp) showed a higher efficiency in silencing of the eGFP gene than did those with a spacer of a GUS gene fragment or a longer intron (850 bp) of a chitin binding protein gene. Application of pSilent-1 to two M. oryzae endogenous genes, MPG1 and polyketide synthase-like gene, resulted in various degrees of silencing of the genes in 70-90% of the resulting transformants. RNA silencing was also induced by a pSilent-1-based vector in Colletotrichum lagenarium at a slightly lower efficiency than in M. oryzae, indicating that this silencing vector should provide a useful reverse genetic tool in ascomycete fungi. (c) 2005 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2005年04月, FUNGAL GENETICS AND BIOLOGY, 42 (4), 275 - 283, 英語

    研究論文(学術雑誌)

  • H Nakayashiki, S Hanada, NB Quoc, N Kadotani, Y Tosa, S Mayama

    We have developed a pHANNIBAL-like silencing vector, pSilent-1, for ascomycete fungi, which carries a hygromycin resistance cassette and a transcriptional unit for hairpin RNA expression with a spacer of a cutinase gene intron from the rice blast fungus Magnaporthe oryzae. In M. oryzae, a silencing vector with the cutinase intron spacer (147 bp) showed a higher efficiency in silencing of the eGFP gene than did those with a spacer of a GUS gene fragment or a longer intron (850 bp) of a chitin binding protein gene. Application of pSilent-1 to two M. oryzae endogenous genes, MPG1 and polyketide synthase-like gene, resulted in various degrees of silencing of the genes in 70-90% of the resulting transformants. RNA silencing was also induced by a pSilent-1-based vector in Colletotrichum lagenarium at a slightly lower efficiency than in M. oryzae, indicating that this silencing vector should provide a useful reverse genetic tool in ascomycete fungi. (c) 2005 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2005年04月, FUNGAL GENETICS AND BIOLOGY, 42 (4), 275 - 283, 英語

    [査読有り]

    研究論文(学術雑誌)

  • いもち病菌の分類とその現状

    土佐 幸雄

    2005年, 植物防疫, 59 45-48, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • NAKAYASHIKI Hitoshi, AWA T, TOSA Yukio, MAYAMA Shigeyuki

    2005年, FEBS letters, 579,488-492, 英語

    研究論文(学術雑誌)

  • H Nakayashiki, T Awa, Y Tosa, S Mayama

    MAGGY is a Ty3/Gypsy retrotransposon, which was identified in the rice blast fungus Magnaporthe oryzae. Some Ty3/Gypsy retrotransposons, including MAGGY, contain a chromodomain-like module (CLM) in the C-terminus of the integrase domain. We have made a series of MAGGY mutants to examine the role of the CLM in the transposition activity of the element. Introduction of a mutation at different positions in the MAGGY integrase revealed that a loss or alteration of the CLM resulted in a drastic decrease in the transposition activity of the element. Our results indicate that the CLM may confer high transposition activity to the element. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2005年01月, FEBS LETTERS, 579 (2), 488 - 492, 英語

    [査読有り]

    研究論文(学術雑誌)

  • WANG Y G, YANG Q, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

    2005年, J. Gen. Plant. Pathol., 未記入, 英語

    研究論文(学術雑誌)

  • WANG Y.Q, YANG Q, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

    2005年, J. Gen. Plant Pathol., 71 33-38, 英語

    [査読有り]

    研究論文(学術雑誌)

  • K Kusaka, Y Tada, T Shigemi, M Sakamoto, H Nakayashiki, Y Tosa, S Mayama

    We have developed an oat cell-free apoptosis system to investigate the execution mechanisms of plant apoptosis. Cell extracts derived from oat tissues undergoing toxin (victorin)-induced apoptosis caused nuclear collapse and internucleosomal DNA fragmentation in isolated nuclei. Pharmacological studies revealed that cysteine protease, which is E-64-sensitive but insensitive to caspase-specific inhibitors, is a crucial component in the morphological change of isolated nuclei, and that nuclease and the cysteine protease act cooperatively to induce the apoptotic DNA laddering. Interestingly, this finding is contrasted with those in well-studied animal cell-free systems in which an apoptotic endonuclease is solely responsible for the DNA fragmentation. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2004年12月, FEBS LETTERS, 578 (3), 363 - 367, 英語

    研究論文(学術雑誌)

  • N Kadotani, H Nakayashiki, Y Tosa, S Mayama

    Dicer is a ribonuclease III-like enzyme playing a key role in the RNA silencing pathway. Genome sequencing projects have demonstrated that eukaryotic genomes vary in the numbers of Dicer-like (DCL) proteins from one ( human) to four ( Arabidopsis). Two DCL genes, MDL-1 and - 2 ( Magnaporthe Dicer-like-1 and - 2) have been identified in the genome of the filamentous fungus Magnaporthe oryzae. Here we show that the knockout of MDL-2 drastically impaired gene silencing of enhanced green fluorescence protein by hairpin RNA and reduced related small interfering RNA ( siRNA) accumulation to nondetectable levels. In contrast, mutating the other DCL, MDL-1, exhibited a gene silencing frequency similar to wild type and accumulated siRNA normally. The silencing-deficient phenotype and loss of siRNA accumulation in the mdl-2 mutant was restored by genetic complementation with the wild-type MDL-2 allele. These results indicate that only MDL-2 is responsible for siRNA production, and no functional redundancy exists between MDL-1 and MDL-2 in the RNA silencing pathway in M. oryzae. Our findings contrast with a recent report in the filamentous fungus Neurospora crassa, where two DCL proteins are redundantly involved in the RNA silencing pathway, but are similar to the results obtained in a more distantly related organism, Drosophila melanogaster, where an individual DCL protein has a distinct role in the siRNA/micro-RNA pathways.

    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2004年10月, JOURNAL OF BIOLOGICAL CHEMISTRY, 279 (43), 44467 - 44474, 英語

    研究論文(学術雑誌)

  • Y Otsu, Y Matsuda, H Mori, H Ueki, T Nakajima, K Fujiwara, M Matsumoto, N Azuma, K Kakutani, T Nonomura, Y Sakuratani, T Shinogi, Y Tosa, S Mayama, H Toyoda

    An entomopathogenic bacterium was isolated from tomato leaves and used as a microbial agent to control larvae of phytophagous ladybird beetles Epilachna vigintioctopunctata. The isolate was identified as Pseudomonas fluorescens KPM-018P on the basis of its bacteriological characteristics. KPM-018P produced extracellular chitinase to form a transparent zone around their colonies by hydrolyzing chitin in a minimal medium. Pale-yellow colonies turned red after a change of incubation temperature. These characteristics were availed as markers for tracking KPM-018P. The bacteria produced biosurfactants that enabled the bacteria to stably colonize the hydrophobic leaf surface; they were recovered without any considerable decrease even after a suspension of KPM-018P was sprayed onto leaves. KPM-018P, transformed with the gfp gene and observed with fluorescence microscopy, stably dwelled in the junctions of epidermal cells of bacteria-sprayed leaves. Ingestion of KPM-018P-sprayed leaves by the larvae caused prompt death of these insects to eventually suppress their pupation. This method is thus effective for decreasing the population of larvae and adult insect pests in the subsequent generation. The study provides an experimental basis for the biocontrol of herbivorous insect pests using a leaf-inhabiting, entomopathogenic strain of P. fluorescens.

    CARFAX PUBLISHING, 2004年08月, BIOCONTROL SCIENCE AND TECHNOLOGY, 14 (5), 427 - 439, 英語

    研究論文(学術雑誌)

  • Nitric oxide and reactive oxygen species do not elicit hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oat.

    TADA Y, MORI T, SHINOGI Takeshi, YAO N, TAKAHASHI S, BETSUYAKU S, SAKAMOTO M, PARK P, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2004年, Molecular Plant-Microbe Interactions., 17 3 245-253, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nitric oxide and reactive oxygen species are not required for hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oats.

    TADA Y, MORI T, SHINOGI T, YAO N, TAKAHASHI S, BETSUYAKU S, SAKAMOTO M, PARK P, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2004年, Mol. Plant-Microbe Interact., 17,245-253, 英語

    研究論文(学術雑誌)

  • TOSA Yukio, HIRATA K, TAMBA H, NAKAGAWA S, CHUMA Izumi, ISOBE Chihiro, OSUE J, URASHIMA A S, DON L. D, KUSABA M, NAKAYASHIKI Hitoshi, TANAKA A, TANI T, MORI Naoki, MAYAMA Shigeyuki

    2004年, Phytopathology, 94,454-462, 英語

    研究論文(学術雑誌)

  • Analysis of the involvement of hydroxyanthranilate hydroxycinnamoyltransferase and caffeoyl-CoA 3-O-methyltransferase in phytoalexin biosynthesis in Oat.

    YANG Q, IMAI S, ISHIHARA A, ZHANG L, TRINH H X, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2004年, Mol. Plant-Microbe Interact., 17,81-89, 英語

    研究論文(学術雑誌)

  • NARUSAKA Y, NARUSAKA M, SEKI M, ISHIDA J, NAKASHIMA M, KAMIYA A, ENJU A, SAKURAI T, SATOH M, KOBAYASHI M, TOSA Yukio, PARK P, SHINOZAKI K

    2003年, Plant and Cell Physiology, 44:377-387, 英語

    研究論文(学術雑誌)

  • NARUSAKA Y, NARUSAKA M, SEKI M, ISHIDA J, NAKASHIMA M, KAMIYA A, ENJU A, SAKURAI T, SATOH M, KOBAYASHI M, TOSA Yukio, PARK P, SHINOZAKI K

    2003年, Plant cell Physiol., 44,377-387, 英語

    研究論文(学術雑誌)

  • Suppression of leaf feeding and oviposition of phytophagous ladybird beetles(coleoptera: Coccinellidae) by chitinase gene-transformed phylloplane bacteria and their specific bacteriophages entrapped in alginate gel beads.

    OTSU Y, MORI H, KOMUTA K, SHIMIZU H, NOGAWA S, MATSUDA Y, NONOMURA T, SAKURATANI Y, TOSA Yukio, MAYAMA Shigeyuki, TOYODA H

    2003年, J. Econ. Entomol., 96,555-563, 英語

    研究論文(学術雑誌)

  • RNA silencing in the phytopathogenic fungus Magnaporthe oryzae.

    KADOTANI Naoki, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2003年, Mol. Plant-Microbe Interact., 16,769-776, 英語

    研究論文(学術雑誌)

  • TAKIKAWA Y, ISHII Y, FUJIKAWA K, MATSUDA Y, NONOMURA T, KAKUTANI K, TOSA Yukio, MAYAMA Shigeyuki, TOYODA H

    2003年, J. Gen. Plant. Pathol., 69,131-137, 英語

    研究論文(学術雑誌)

  • Nitric oxide and reactive oxygen species are not required for hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oats.

    TADA Y, MORI T, SHINOGI T, YAO N, TAKAHASHI S, BETSUYAKU S, SAKAMOTO M, PARK P, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2003年, Mol. Plant-MicrobeInteract., 未記入, 英語

    研究論文(学術雑誌)

  • Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae.

    KADOTANI Naoki, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2003年, Mol. Plant-MicrobeInteract., 16:769-776, 英語

    研究論文(学術雑誌)

  • Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae.

    CHUMA Izumi, TOSA Yukio, TAGA M, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

    2003年, Curr. Genet., 43:191-198., 英語

    研究論文(学術雑誌)

  • TOSA Yukio, HIRATA K, TAMBA H, NAKAGAWA S, CHUMA Izumi, ISOBE C, OSUE J, URASHIMA A S, DON L D, KUSABA M, NAKAYASHIKI Hitoshi, TANAKA A, TANI T, MORI Naoki, MAYAMA Shigeyuki

    2003年, Mol. Plant-MicrobeInteract., 未記入, 英語

    研究論文(学術雑誌)

  • IIDA Y, MATSUDA Y, SAITO R, NAKASATO M, NONOMURA T, KAKUTANI K, TOSA Yukio, MAYAMA Shigeyuki, TOYODA H

    2003年, Biosci. Biotechnol. Biochem., 67,198-202, 英語

    研究論文(学術雑誌)

  • OTSU Y, MATSUDA Y, SHIMIZU H, UEKI H, MORI H, FUJIWARA K, NAKAJIMA T, MIWA A, NONOMURA T, SAKURATANI Y, TOSA Yukio, MAYAMA Shigeyuki, TOYODA H

    2003年, J. Appl. Entomol., 127,441-446, 英語

    研究論文(学術雑誌)

  • Analysis of the involvement of hydroxyanthranilate hydroxycinnamoyltransferase and caffeoyl-CoA 3-O-methyltransferase in phytoalexin biosynthesis in Oat.

    YANG Q, IMAI S, ISHIHARA A, ZHANG L, TRINH H.X, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    2003年, Mol. Plant-MicrobeInteract., 未記入, 英語

    研究論文(学術雑誌)

  • Yao N, Tada Y, Sakamoto M, Nakayashiki H, Park P, Tosa Y, Mayama S

    2002年06月, The Plant journal : for cell and molecular biology, 30 (5), 567 - 579

    [査読有り]

  • Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains

    Nakayashiki, Hitoshi, Matsuo, Hideki, Chuma, Izumi, Ikeda, Kenichi, Betsuyaku, Shigeyuki, Kusaba, Motoaki, Tosa, Yukio, Mayama, Shigeyuki

    2001年10月, Nucleic Acids Res., 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • イネいもち病菌非病原力遺伝子のrepeat‐poor領域における欠失

    平岡大輝, 荒添貴之, 曾根輝雄, 土佐幸雄, 中馬いづみ

    2019年03月05日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2019, 59, 日本語

  • Pyricularia graminis-tritici is not the correct species name for the wheat blast fungus: response to Ceresini et al.

    B. Valent, M. Farman, 土佐 幸雄, D. Begerow, E. Fournier, P. Gladieux, M.T. Islam, S. Kamoun, M. Kemler, L.M. Kohn, M.-H. Lebrun, J.E. Stajich, N.J. Talbot, R. Terauchi, D. Tharreau, N. Zhang

    2019年, Mol. Plant Pathol., 20 (2), 173 - 179, 英語

    [査読有り][招待有り]

  • ゲノムを切らずに書き換えるイネいもち病菌における新規ゲノム編集

    荒添貴之, 西田敬二, 田畑麻由良, 平岡大輝, 中馬いづみ, 土佐幸雄, 近藤昭彦

    2017年04月12日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 75, 日本語

  • エンバクいもち病菌のコムギ品種Hopeに対する非病原力遺伝子のクローニング

    堀江晶子, 小松香織, 東夏希, 村上翼, 足助聡一郎, 中馬いづみ, 土佐幸雄

    2017年04月12日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 45, 日本語

  • いもち病菌集団内エフェクター分布解析によるシコクビエいもち病菌の普通系コムギに対する新規非病原力遺伝子のクローニング

    足助聡一郎, MAGCULIA Nicole, 中馬いづみ, 土佐幸雄

    2017年04月12日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 44, 日本語

  • Brassica rapa L.におけるアブラナ科植物白さび病抵抗性遺伝子の探索と検出

    西本良太, 宮路直実, 藤本龍, 土佐幸雄, 中馬いづみ

    2017年04月12日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 64, 日本語

  • コムギいもち病菌の保有するシコクビエに対する非病原力遺伝子の検出と分子マッピング

    天藤陽香, 足助聡一郎, 村上翼, 中馬いづみ, 土佐幸雄

    2017年04月12日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 45, 日本語

  • アブラナ科植物白さび病菌Albugo candidaに対する抵抗性遺伝子同定に向けたBrassica rapa抵抗性品種の選抜

    西本良太, 宮路直実, 駒寛子, 藤本龍, 土佐幸雄, 中馬いづみ

    2016年09月24日, 育種学研究, 18, 115, 日本語

  • コムギいもち病菌のオオムギに対する非病原力遺伝子PBY2の分子マッピング

    村上翼, 西見周子, 中馬いづみ, 土佐幸雄

    2016年03月03日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2016, 59, 日本語

  • シコクビエいもち病菌が保有するPWT3ホモログの普通系コムギに対する非病原力遺伝子としての機能の検討

    足助聡一郎, 天藤陽香, TRINH Vy, 井上喜博, 井上喜博, 中馬いづみ, 土佐幸雄

    2016年03月03日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2016, 60, 日本語

  • エンバクに対するコムギいもち病菌の非病原力遺伝子PAT1を保有するBACクローンの同定

    東夏希, 森亮太, 井上喜博, 中馬いづみ, 土佐幸雄

    2016年03月03日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2016, 59, 日本語

  • 日本産メヒシバいもち病原因菌の分類学的位置付けに関する検討

    田原佳代子, 草場基章, 中馬いづみ, 土佐幸雄

    2016年03月03日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2016, 78, 日本語

  • エンバクいもち病菌に対するコムギの抵抗性遺伝子RwtXAおよびRwtXDの同定

    小松香織, 井上喜博, VY Trinh Thi Phuong, 中馬いづみ, 土佐幸雄

    2016年, 日本植物病理学会報, 82 (1), 52(J‐STAGE), 日本語

  • シコクビエいもち病菌の普通系コムギに対する非病原力遺伝子クローニングのためのBC1F1集団の作出および対応する抵抗性遺伝子の座乗候補染色体の検討

    足助聡一郎, 中馬いづみ, 土佐幸雄

    2016年, 日本植物病理学会報, 82 (1), 52(J‐STAGE), 日本語

  • Colletotrichum nymphaeae(Passerini)Aaによるニセアカシア炭疽病(病原追加)

    山岸菜穂, 佐藤豊三, 石山佳幸, 小木曽秀紀, 中馬いづみ, 土佐幸雄

    2015年03月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2015, 49, 日本語

  • エンバクいもち病菌のコムギ品種Hopeに対する非親和性に関与する遺伝子対の同定

    小松香織, 森亮太, 井上喜博, TRINH Vy, 中馬いづみ, 土佐幸雄

    2015年03月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2015, 89, 日本語

  • DNA二本鎖切断とその修復過程において生じるイネいもち病菌の病原性変異

    荒添貴之, 田中寿樹, 小川哲央, 三好健之介, 大和澄, 佐久間哲史, 山本卓, 有江力, 中馬いづみ, 大里修一, 土佐幸雄, 桑田茂

    2015年03月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2015, 62, 日本語

  • ペレニアルライグラスいもち病菌のコムギに対する非病原力遺伝子PWT3のコムギいもち病菌における変異とRwt3保有コムギ品種への適応

    井上喜博, TRINH V, 吉田健太郎, 寺内良平, 中馬いづみ, 土佐幸雄

    2015年03月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2015, 55, 日本語

  • シコクビエいもち病菌が保有する普通系コムギに対する非病原力遺伝子数の遺伝学的推定

    足助聡一郎, 西見周子, TRINH Vy, 井上喜博, 中馬いづみ, 土佐幸雄

    2015年03月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2015, 56, 日本語

  • 非病原力遺伝子PWT3の破壊によるライグラスいもち病菌のコムギへの病原性獲得

    TRINH V, 井上喜博, 吉田健太郎, 草場基章, 寺内良平, 中馬いづみ, 土佐幸雄

    2015年03月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2015, 55, 日本語

  • いもち病菌における新菌群分化機構の解析

    土佐幸雄, 井上喜博, VY Trinh Thi Phuong, 中馬いづみ

    2014年07月28日, 日本植物病理学会植物感染生理談話会論文集, (49), 21 - 28, 日本語

  • ムギ類とその近縁植物におけるイネいもち病菌抵抗性遺伝子の探索

    角圭人, 中馬いづみ, 土佐幸雄

    2014年05月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2014, 76, 日本語

  • クローナルに増殖したと考えられる三重県のMBI‐D剤耐性イネいもち病菌株集団から観察されたMAGGY‐DNAフィンガープリントパターンの変異

    宮司貴浩, 池上裕美子, 鈴木啓史, 中馬いづみ, 土佐幸雄, 草場基章

    2014年05月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2014, 83, 日本語

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT3のクローニングおよびその進化過程の解析

    TRINH Vy, 井上喜博, 吉田健太郎, 寺内良平, 中馬いづみ, 土佐幸雄

    2014年05月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2014, 90, 日本語

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT4のクローニングおよびその進化過程の解析

    井上喜博, TRINH Vy, 吉田健太郎, 三岡周子, 麻野北斗, 寺内良平, 中馬いづみ, 土佐幸雄

    2014年05月10日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2014, 90, 日本語

  • Yukio Tosa, Izumi Chuma

    Pyricularia oryzae (Magnaporthe oryzae), a causal agent of blast diseases on staple gramineous crops, is a model organism listed as the most important economically and scientifically of the top 10 fungal pathogens by fungal molecular pathologists. Although we are now in an era of genome-enabled analysis, we need to understand the history of the pathogen's taxonomy, classification, and parasitic specialization in addition to recent research advances. In this review, we focus on these rather fundamental topics. First, the history of classification, including the discovery of its sexual stage and designation, is overviewed. Based on recent results of phylogenetic analysis of Magnaporthaceae isolates, blast fungi are suggested to constitute a robust population that is not congeneric with Magnaporthe salvinii, the type species of Magnaporthe. Second, genetic mechanisms involved in its parasitic specialization into host-specific subgroups and races are outlined. Implications of recent molecular data for resistance breeding are discussed.

    SPRINGER JAPAN KK, 2014年05月, JOURNAL OF GENERAL PLANT PATHOLOGY, 80 (3), 202 - 209, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • いもち病菌の分類と寄生性分化

    土佐 幸雄, 中馬 いづみ

    2014年, 日本植物病理学界報, 日本語

    [査読有り][招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 国際植物命名規約改訂に伴ういもち病菌の属名の再検討

    村田暢明, 草場基章, 青木孝之, 土佐幸雄, 中馬いづみ

    2013年03月21日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2013, 78, 日本語

  • いもち病菌非病原力遺伝子AVR‐Piztの変異に関わるトランスポゾン挿入と点突然変異

    角圭人, 中馬いづみ, 草場基章, 本藏良三, 土佐幸雄

    2013年03月21日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2013, 64, 日本語

  • コムギのエンバクいもち菌・ペレニアルライグラスいもち菌に対する抵抗性に共通に関与する抵抗性遺伝子‐非病原力遺伝子ペアの同定

    TRINH T.P.V, VU L.A, CUMAGUN C.J.R, 井上喜博, 中馬いづみ, 土佐幸雄

    2013年03月21日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2013, 64, 日本語

  • イネいもち病菌のクローナル増殖時に観察されたMAGGY‐DNAフィンガープリントパターンの変異

    池上裕美子, 宮司貴浩, 鈴木啓史, 中馬いづみ, 土佐幸雄, 草場基章

    2013年03月21日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2013, 87, 日本語

  • 次世代シーケンサーを用いたエンバクいもち病菌のコムギに対する非病原力遺伝子PWT4の同定

    井上喜博, 吉田健太郎, 三岡周子, 麻野北斗, 寺内良平, 中馬いづみ, 土佐幸雄

    2013年03月21日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2013, 64, 日本語

  • MBI‐D剤耐性に関与するSDH遺伝子のPyricularia属菌集団における変異性解析

    村田暢明, 中馬いづみ, 鈴木啓史, 草場基章, 土佐幸雄

    2013年02月25日, 日本植物病理学会報, 79 (1), 40 - 41, 日本語

  • オオムギのいもち病抵抗性遺伝子座Rmo2に対応するアワいもち病菌の非病原力遺伝子PBY3,PBY4の同定と分子マッピング

    中村貴弘, HYON G.‐S, NGUYEN Thi, Thanh Nga, 井上喜博, 中馬いづみ, 土佐幸雄

    2013年02月25日, 日本植物病理学会報, 79 (1), 40, 日本語

  • イネ科植物いもち病菌集団における宿主抵抗性遺伝子への適応戦略

    中馬 いづみ, 土佐 幸雄

    2013年, 植物防疫, 67 (2), 94 - 99, 日本語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • いもち病菌非病原力遺伝子AVR‐Piztの特殊性とそのエフェクター機能の重要性に関する一考察

    角圭人, 中馬いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2012年03月15日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2012, 79, 日本語

  • いもち病菌非病原力遺伝子のMultiple translocationはなぜ起こったか?欠失/再獲得仮説

    中馬いづみ, 古田純一, 草場基章, 吉田健太郎, 寺内良平, 藤田佳克, 中屋敷均, VALENT Barbara, 土佐幸雄

    2012年03月15日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2012, 79, 日本語

  • いもち病菌集団Pyricularia spp.における非病原力遺伝子AVR‐Pikの染色体彷徨―特に過剰染色体の関与について

    中馬いづみ, 今井誉子, 古田純一, 角圭人, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2011年03月11日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 57, 日本語

  • いもち病菌集団Pyricularia spp.における非病原力遺伝子AVR‐PiaおよびAVR‐Piiの染色体彷徨

    古田純一, 中馬いづみ, 大窪貴子, 草場基章, 吉田健太郎, 寺内良平, 曾根輝雄, 土佐幸雄

    2011年03月11日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 142, 日本語

  • 非病原力遺伝子AVR‐Piztはいもち病菌集団において安定して第7染色体に存在する

    角圭人, 中馬いづみ, 古田純一, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2011年03月11日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 142, 日本語

  • オオムギの各種いもち病菌抵抗性に関与する複合遺伝子座Rmo2の同定と分子マッピング

    HYON G, NGUYEN Thi, Thanh Nga, 中馬いづみ, 岡田和馬, 佐藤和広, 土佐幸雄

    2011年03月11日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 52, 日本語

  • 野生イネ菌,アワ菌,コムギ菌を用いた比較SSRマッピングによるいもち病菌ゲノム構造の解析

    堀田祐麻, 中馬いづみ, 内藤大貴, 草場基章, 土佐幸雄

    2011年03月11日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 143, 日本語

  • いもち病菌集団における非病原力遺伝子AVR‐Piiファミリーの解析

    古田純一, 中馬いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2011年02月25日, 日本植物病理学会報, 77 (1), 62, 日本語

  • SSRマーカーおよび分泌タンパク質コード遺伝子を用いたイネいもち病菌連鎖地図の改良

    内藤大貴, 諸石雅彦, 中馬いづみ, 吉田健太郎, 寺内良平, 土佐幸雄, 藤田佳克, 草場基章

    2011年02月25日, 日本植物病理学会報, 77 (1), 37, 日本語

  • 日本産イネいもち病菌におけるPex31ホモログコード遺伝子が座乗する余剰染色体の特徴付け

    持田泰雅, 馬場崎翔一, 中馬いづみ, 吉田健太郎, 寺内良平, 土佐幸雄, 藤田佳克, 草場基章

    2011年02月25日, 日本植物病理学会報, 77 (1), 36 - 37, 日本語

  • いもち病菌サブテロメアにおいて高頻度に検出される遺伝子変換

    中馬いづみ, 茨木加奈, 土佐幸雄

    2011年02月25日, 日本植物病理学会報, 77 (1), 62, 日本語

  • いもち病菌非病原力遺伝子ファミリーの進化と抵抗性崩壊

    中馬いづみ, 草場基章, 土佐幸雄

    2010年07月23日, 日本植物病理学会植物感染生理談話会論文集, (46), 91 - 99, 日本語

  • イネいもち病菌84R‐62BとY93‐245c‐2の子孫菌株で観察されたPex31ホモログの転座

    持田泰雅, 橋川慎吾, 成富毅誌, 中馬いづみ, 吉田健太郎, 寺内良平, 土佐幸雄, 藤田佳克, 草場基章

    2010年03月30日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 154, 日本語

  • いもち病菌の種特異的寄生性に関与する非病原力遺伝子PWT1の染色体座乗領域

    中馬いづみ, ZHAN S.‐W, 浅野峻介, NGUYEN N.T.T, TRINH V.T.P, 白井倫子, 茨木加奈, 土佐幸雄

    2010年03月30日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 74, 日本語

  • アワいもち病菌のコムギに対する非病原力遺伝子PWT1周辺領域のクローニング

    岡田和馬, HYON G, 白井倫子, ZHAN S, 中馬いづみ, 土佐幸雄

    2010年03月30日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 155, 日本語

  • 日本産イネいもち病菌のゲノムに分布するPex31ホモログの数

    成富毅誌, 馬場崎翔一, 持田泰雅, 今井誉子, 中馬いづみ, 吉田健太郎, 寺内良平, 土佐幸雄, 藤田佳克, 草場基章

    2010年03月30日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 155, 日本語

  • Super‐SAGE法を用いたエンバクいもち病菌のコムギに対する非病原力遺伝子PWT4の候補遺伝子の選抜

    井上喜博, 麻野北斗, 中馬いづみ, 吉田健太郎, 松村英生, 寺内良平, 土佐幸雄

    2010年02月25日, 日本植物病理学会報, 76 (1), 62, 日本語

  • イネいもち病菌株84R‐62B,Y93‐245c‐2およびその子孫菌株F1‐327におけるPex31ホモログの座乗染色体

    持田泰雅, 成富毅誌, 中馬いづみ, 吉田健太郎, 寺内良平, 土佐幸雄, 藤田佳克, 草場基章

    2010年02月25日, 日本植物病理学会報, 76 (1), 28, 日本語

  • いもち病菌小分生子の細胞学的特徴

    中馬いづみ, 中屋敷均, 土佐幸雄

    2010年02月25日, 日本植物病理学会報, 76 (1), 61, 日本語

  • 分泌タンパク質遺伝子のマッピングによるいもち病菌染色体高度可変領域の同定

    浅野峻介, 中馬いづみ, 古田純一, 今井誉子, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2010年02月25日, 日本植物病理学会報, 76 (1), 72, 日本語

  • さまざまないもち病菌菌株における非病原力遺伝子AVR‐Piiの周辺構造

    古田純一, 中馬いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2010年02月25日, 日本植物病理学会報, 76 (1), 72, 日本語

  • イネいもち病菌株84R‐62BおよびY93‐245c‐2の交配系におけるPex31ホモログの遺伝分離

    成富毅誌, 持田泰雅, 中馬いづみ, 吉田健太郎, 寺内良平, 土佐幸雄, 藤田佳克, 草場基章

    2010年02月25日, 日本植物病理学会報, 76 (1), 28, 日本語

  • Yukio Tosa

    SPRINGER TOKYO, 2009年12月, JOURNAL OF GENERAL PLANT PATHOLOGY, 75 (6), 455 - 457, 英語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • いもち病菌集団における非病原力遺伝子AVR‐Piiの分布

    古田純一, 中馬いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐幸雄

    2009年08月25日, 日本植物病理学会報, 75 (3), 253, 日本語

  • いもち病菌における非病原力遺伝子の比較解析とその進化過程に関する考察

    中馬いづみ, 草場基章, 大窪貴子, 古田純一, 萬野英俊, 吉田健太郎, 寺内良平, 曾根輝雄, 土佐幸雄

    2009年08月25日, 日本植物病理学会報, 75 (3), 196, 日本語

  • Setaria属寄生性いもち病菌におけるAVR‐Pitaの分布

    萬野英俊, 中馬いづみ, 祝千尋, 諸石雅彦, 草場基章, 土佐幸雄

    2009年08月25日, 日本植物病理学会報, 75 (3), 252, 日本語

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT4へのクロモソームウォーキング

    井上喜博, HAU V. T. B, 中馬いづみ, 土佐幸雄

    2009年08月25日, 日本植物病理学会報, 75 (3), 251 - 252, 日本語

  • AFLP分析を用いたSetaria属植物から分離したいもち病菌株の系統解析

    祝千尋, 諸石雅彦, 山頭亜紀子, 中馬いづみ, 土佐幸雄, 草場基章

    2009年02月25日, 日本植物病理学会報, 75 (1), 40 - 41, 日本語

  • いもち病菌集団における非病原力遺伝子AVR‐Piaの分布

    大窪貴子, 中馬いづみ, 吉田健太郎, 中屋敷均, 寺内良平, 土佐幸雄

    2009年02月25日, 日本植物病理学会報, 75 (1), 64, 日本語

  • いもち病菌サブテロメア領域における構造変異

    中馬いづみ, 中屋敷均, 土佐幸雄

    2009年02月25日, 日本植物病理学会報, 75 (1), 65, 日本語

  • 非病原力遺伝子としての機能からみたいもち病菌AVR‐Pitaの起源と進化

    中馬いづみ, 吉田健太郎, 中屋敷均, 眞山滋志, 土佐幸雄

    2008年08月25日, 日本植物病理学会報, 74 (3), 192 - 193, 日本語

  • イネいもち病菌84R‐62BおよびY93‐245c‐2の交配系における非病原性遺伝子AurPitaの染色体座乗位置について

    大鷹和也, 中馬いづみ, 土佐幸雄, 草場基章

    2007年11月10日, 九州病害虫研究会報, 53, 124, 日本語

  • いもち病菌非病原力遺伝子AVR‐Pitaホモログのタイピング,マッピングおよび機能の比較解析

    中馬いづみ, 大鷹和也, 草場基章, 吉田健太郎, 中屋敷均, 眞山滋志, 土佐幸雄

    2007年08月25日, 日本植物病理学会報, 73 (3), 202, 日本語

  • いもち病菌小型分生子の病理学的役割を解明するための基礎調査

    細木直樹, 中馬いづみ, 篠木武, 池田健一, 中屋敷均, 眞山滋志, 土佐幸雄, PARK P

    2007年08月25日, 日本植物病理学会報, 73 (3), 213, 日本語

  • いもち病菌非病原力遺伝子の変異と彷徨

    土佐幸雄, 中馬いづみ

    2007年07月27日, 日本植物病理学会植物感染生理談話会論文集, (43), 117 - 125, 日本語

  • いもち病菌非病原力遺伝子Avr‐Pitaの座乗染色体変動過程とその機構

    中馬いづみ, 吉田健太郎, 磯部千尋, 土佐幸雄, 中屋敷均, 眞山滋志

    2006年11月25日, 日本植物病理学会報, 72 (4), 242, 日本語

  • エンバクにおける細胞死機構研究のこれまで

    中屋敷 均, 土佐 幸雄, 眞山 滋志

    2004年, 植物病の探求. 高松進ら編 「植物病の探求」, 48-53, 日本語

    その他

  • シロイヌナズナにおけるgeneral resistanceの解析

    鳴坂 義弘, 篠崎 一雄, 朴 杓允, 鳴坂 真理, 土佐 幸雄, 関 原明, 石田 順子, 中嶋 舞子, 槐 亜希子, 櫻井 哲也, 佐藤 将一

    植物の本質的な防御機構を解明するため、ニホンナシに感染する病原糸状菌<I>Alternaria alternata</I> Japanese pear pathotypeとシロイヌナズナを用いて研究を行った。<I>A. alternata</I>をシロイヌナズナ葉に接種したところ、胞子は葉上で発芽して付着器を形成し、penetration pegを形成したが、植物細胞内へ侵入することは出来ず、感染は成立しなかった。これに対して、植物は菌の侵入を感知し、速やかにパピラを形成したが、活性酸素の生成や、過敏感細胞死等の過敏感反応を示さなかった。一方、cDNAマイクロアレイを用いて<I>A. alternata</I>の攻撃に対するシロイヌナズナの発現遺伝子の網羅的解析を行った。その結果、128の遺伝子が菌接種により発現誘導され、86遺伝子が発現抑制された。本報告において、これら遺伝子群の防御応答における役割について考察する。

    日本植物生理学会, 2003年, 日本植物生理学会年会およびシンポジウム 講演要旨集, 2003 (0), 612 - 612

書籍等出版物

  • 病原性にかかわる遺伝子

    池田 健一, 土佐 幸雄

    その他, 朝倉書店, 2013年, 日本語

    学術書

  • 「いもち病菌非病原力遺伝子ファミリーの進化と抵抗性崩壊」In 植物感染生理談話会論文集(第46号) 農業現場の問題解決に向けた感染生理学.p.91-99.

    中馬 いづみ, 草場 基章, 土佐 幸雄

    その他, 日本植物病理学会.東京., 2010年, 日本語

    学術書

  • Genetic analyses of “host species specificity” of Magnaporthe oryzae/grisea. In: T. Wolpert, T. Shiraishi, A. Collmer, K. Akimitsu, and J. Glazebrook. (eds) Genome-Enabled Integration of Research in Plant-Pathogen Systems. APS press, Minnesota. p.93-99.

    Tosa Yukio, Chuma Izumi

    その他, APS Press, 2010年, 英語

    学術書

  • Searching for effectors of Magnaporthe oryzae: a multi-faceted genomics approach. In: Rice Blast (Wang, G.-L. and Pang eds.) Springer. pp. 105-112.

    Terauchi, R, Win, J, Kamoun, S, Matsumura, H, Saitoh, H, Kanzaki, H, Yoshida, K, Shenton, M, Berberich, T, Fujisawa, S, Ito, A, Takano, Y, Tosa Yukio

    その他, Springer, 2009年, 英語

    学術書

  • いもち病菌におけるRNAサイレンシング機構 自他識別と応答のバイオフロンティア 植物感染生理談話論文集. 高橋英樹,羽柴輝良編 pp.53-62

    中屋敷 均, 角谷 直樹, 花田 周吾, 土佐 幸雄, 眞山 滋志

    共著, 日本植物病理学会, 2004年, 日本語

    学術書

  • RNA silencing in the phytopathogenic fungus Magnaporthe grisea. In: Rice Blast: Interaction with Rice and Control(eds. S. Kawasaki) pp.15-21

    NAKAYASHIKI Hitoshi, KADOTANI Naoki, TOSA Yukio, MAYAMA Shigeyuki

    共著, Kluwer academic pub., 2004年, 英語

    学術書

  • 遺伝・変異・進化. In 世界におけるいもち病研究の軌跡. 浅賀宏一・加藤肇・山田昌雄・吉野嶺一編pp.11-15

    土佐 幸雄, 鎌倉 高志

    共著, 日本植物防疫協会, 2003年, 日本語

    学術書

講演・口頭発表等

  • コムギいもち病菌の進化機構

    土佐 幸雄

    日本植物病理学会関西部会, 2018年09月, 日本語, 山口大学, 国内会議

    口頭発表(基調)

  • オオムギの各種いもち病菌群に対する抵抗性遺伝子座Rmo2の1アリル候補配列の機能解析

    庭本 大輔, 足助 聡一郎, Analiza Grubanzo Tagle, 久野裕, 佐藤和広, 土佐 幸雄

    日本植物病理学会関西部会, 2018年09月, 日本語, 山口大学, 国内会議

    口頭発表(一般)

  • Mechanisms of evolution of the wheat blast fungus

    土佐 幸雄, 井上 喜博, TRINH THI, PHUONG VY, 寺内 良平, BARBARA VALENT, MARK L. FARMAN

    11th International Coggress of Plant Pathology, 2018年08月, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • コムギいもち病菌に対する新規抵抗性遺伝子の探索

    王 士臻, 足助 聡一郎, TRINH THI, PHUONG VY, 井上 喜博, 中馬 いづみ, 土佐 幸雄

    平成29年度日本植物病理学会関西部会, 2017年09月, 英語, 大阪府立大学, 国内会議

    口頭発表(一般)

  • コムギいもち病菌の保有するシコクビエに対する非病原力遺伝子の検出と分子マッピング

    天藤 陽香, 足助 聡一郎, 村上 翼, 中馬 いづみ, 土佐 幸雄

    平成29年度日本植物病理学会大会, 2017年04月, 日本語, いわて県民情報交流センター, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌のコムギ品種Hopeに対する非病原力遺伝子のクローニング

    堀江 晶子, 小松 香織, 東 夏希, 村上 翼, 足助 聡一郎, 中馬 いづみ, 土佐 幸雄

    平成29年度日本植物病理学会大会, 2017年04月, 日本語, いわて県民情報交流センター, 国内会議

    口頭発表(一般)

  • いもち病菌集団内エフェクター分布解析によるシコクビエいもち病菌の普通系コムギに対する新規非病原力遺伝子のクローニング

    足助 聡一郎, NICOLE MAGCULIA, 中馬 いづみ, 土佐 幸雄

    平成29年度日本植物病理学会大会, 2017年04月, 日本語, いわて県民情報交流センター, 国内会議

    口頭発表(一般)

  • Molecular mechanisms of host jump of Pyricularia oryzae

    土佐 幸雄, 井上 喜博, Trinh T. P. Vy, 中馬 いづみ

    The 7th International Rice Blast Conference, 2016年10月, 英語, Manila, The Philippines, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Cloning of an avirulence gene of an Eleusine isolate of Pyricularia oryzae against common wheat

    中馬 いづみ, 足助 聡一郎, Nicole John Magculia, 土佐 幸雄

    The 7th International Rice Blast Conference., 2016年10月, 英語, Alabang, Philippines, 国際会議

    ポスター発表

  • TALENにより異なるゲノム領域に導入されたイネいもち病菌非病原力(AVR)遺伝子の変異頻度比較解析

    平岡 大輝, 荒添 貴之, 土佐 幸雄, 中馬 いづみ

    平成28年度植物感染生理談話会, 2016年08月, 日本語, 国内会議

    ポスター発表

  • Identification of gene pairs involved in the incompatibility between Eleusine isolates of Pyricularia oryzae and common wheat.

    足助 聡一郎, 中馬 いづみ, 土佐 幸雄

    IS-MPMI XVII Congress, 2016年07月, 英語, Portland, Oregon, U.S.A., 国際会議

    ポスター発表

  • 日本産メヒシバいもち病原因菌の分類学的位置付けに関する検討

    田原 佳代子, 草場 基章, 中馬 いづみ, 土佐 幸雄

    平成28年度日本植物病理学会大会, 2016年, 日本語, 岡山コンベンションセンター, 国内会議

    口頭発表(一般)

  • シコクビエいもち病菌が保有するPWT3ホモログの普通系コムギに対する非病原力遺伝子としての機能の検討.

    足助 聡一郎, 天藤 陽香, Vy Trinh, 井上 喜博, 中馬 いづみ, 土佐 幸雄

    平成28年度日本植物病理学会大会, 2016年, 日本語, 岡山コンベンションセンター, 国内会議

    口頭発表(一般)

  • コムギいもち病菌のオオムギに対する非病原力遺伝子PBY2の分子マッピング

    村上 翼, 西見 周子, 中馬 いづみ, 土佐 幸雄

    平成28年度日本植物病理学会大会, 2016年, 日本語, 岡山コンベンションセンター, 国内会議

    口頭発表(一般)

  • エンバクに対するコムギいもち病菌の非病原力遺伝子PAT1を保有するBACクローンの同定

    東 夏希, 森 亮太, 井上 喜博, 中馬 いづみ, 土佐 幸雄

    平成28年度日本植物病理学会大会, 2016年, 日本語, 岡山コンベンションセンター, 国内会議

    口頭発表(一般)

  • シコクビエいもち病菌の普通系コムギに対する非病原力遺伝子クローニングのためのBC1F1集団の作出および対応する抵抗性遺伝子の座乗候補染色体の検討

    足助 聡一郎, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会関西部会, 2015年09月, 日本語, あわぎんホール徳島県郷土文化会館, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌に対するコムギの抵抗性遺伝子RwtXAおよびRwtXDの同定

    小松 香織, 井上 喜博, Trin Thi, Phuong Vy, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会関西部会, 2015年09月, 日本語, 国内会議

    口頭発表(一般)

  • 非病原力遺伝子PWT3の破壊によるライグラスいもち病菌のコムギへの病原性獲得

    Trinh Thi, Phuong Vy, 井上 喜博, 吉田 健太郎, 草場 基章, 寺内 良平, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会大会, 2015年, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • ペレニアルライグラスいもち病菌のコムギに対する非病原力遺伝子PWT3のコムギいもち病菌における変異とRwt3保有コムギ品種への適応

    井上 喜博, Trinh Thi, Phuong Vy, 吉田 健太郎, 寺内 良平, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会大会, 2015年, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌のコムギ品種Hopeに対する非親和性に関与する遺伝子対の同定

    小松 香織, 森 亮太, 井上 喜博, Trinh Thi, Phuong Vy, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会大会, 2015年, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • Genetic and molecular analyses of the incompatibility between Lolium isolates of Pyricularia oryzae and wheat

    Yukio Tosa, Yoshihiro Inoue, Trinh Thi, Phuong Vy, Izumi Chuma

    11th US-Japan Scientific Seminar, 2015年, 英語, Takamatsu City, Kagawa, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Fine-mapping of Rmo2, a resistance locus against the blast fungus in barley

    Tagle A, Hyon, G-S, Yamaji N, Hisano H, Sato K, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会大会, 2015年, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • Colletotrichum nymphaeae  (Passerini) Aa によるニセアカシア炭疽病(病原追加)

    山岸 菜穂, 佐藤 豊三, 石山 佳幸, 小木曽 秀紀, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会大会, 2015年, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • Cloning of AVR-Rmg8 corresponding to Rmg8, a wheat gene for resistance to Triticum isolates of Pyricularia oryzae

    Anh V.L, 井上 喜博, Nguyen A.T, Trinh Thi, Phuong Vy, 中馬 いづみ, 土佐 幸雄

    平成27年度日本植物病理学会大会, 2015年, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • Evolution of the genus Pyricularia, the rice blast fungus, and its effector genes

    中馬 いづみ, 青木 孝之, 土佐 幸雄

    The 10th International Mycological Congress, 2014年08月, 英語, Bangkok, Thailand, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Chromosomal Localization Patterns of Avirulence Effector Genes in the Rice Blast Fungus Pyricularia oryzae and Related Species

    中馬 いづみ, 角 圭人, 土井 佑介, 村井 俊子, 草場 基章, 吉田 健太郎, 寺内 良平, 中屋敷 均, 土佐 幸雄

    IS-MPMI 2014 International Congress, 2014年07月, 英語, Rhodes, Greece, 国際会議

    ポスター発表

  • ムギ類とその近縁植物におけるイネいもち病菌抵抗性遺伝子の探索

    角 圭人, 中馬 いづみ, 土佐 幸雄

    平成26年度日本植物病理学会大会, 2014年06月, 日本語, 札幌コンベンションセンター, 国内会議

    口頭発表(一般)

  • クローナルに増殖したと考えられる三重県のMBI-D剤耐性イネいもち病菌株集団から観察されたMAGGY-DNAフィンガープリントパターンの変異

    宮司 貴浩, 池上 裕美子, 鈴木 啓史, 中馬 いづみ, 土佐 幸雄, 草場 基章

    平成26年度日本植物病理学会大会, 2014年06月, 日本語, 札幌コンベンションセンター, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT4のクローニングおよびその進化過程の解析

    井上 喜博, Trinh Thi, Phuong Vy, 吉田 健太郎, 三岡 周子, 麻野 北斗, 寺内 良平, 中馬 いづみ, 土佐 幸雄

    平成26年度日本植物病理学会大会, 2014年06月, 日本語, 札幌コンベンションセンター, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT3のクローニングおよびその進化過程の解析

    Trinh Thi, Phuong Vy, 井上 喜博, 吉田 健太郎, 寺内 良平, 中馬 いづみ, 土佐 幸雄

    平成26年度日本植物病理学会大会, 2014年06月, 英語, 札幌コンベンションセンター, 国内会議

    口頭発表(一般)

  • イネ品種「ひとめぼれ」と「蒙古稲」間に認められるイネいもち病菌感染性決定因子の遺伝学的解析

    藤崎 恒喜, 阿部 善子, 高木 宏樹, 阿部 陽, 小林 光智恵, 齋藤 宏昌, 中馬 いづみ, 土佐 幸雄, 寺岡 徹, 寺内 良平

    平成26年度日本植物病理学会大会, 2014年06月, 日本語, 札幌コンベンションセンター, 国内会議

    口頭発表(一般)

  • Various species of Pyricularia constitute a robust clade distinct from Magnaporthe salvinii and Its relatives in the Magnaporthaceae.

    中馬 いづみ, N. Murata, T. Aoki, M. Kusaba, 土佐 幸雄

    International Rice Blast Conference 2013, 2013年08月, 英語, Jejudo, South Korea, 国際会議

    口頭発表(一般)

  • 次世代シーケンサーを用いたエンバクいもち病菌のコムギに対する非病原力遺伝子PWT4の同定

    井上 喜博, 吉田 健太郎, 三岡 周子, 麻野 北斗, 寺内 良平, 中馬 いづみ, 土佐 幸雄

    平成25年度日本植物病理学会大会, 2013年, 日本語, 岐阜大学, 国内会議

    口頭発表(一般)

  • 国際植物命名規約改定に伴ういもち病菌の属名の再検討

    村田 暢明, 草場 基章, 青木 孝之, 土佐 幸雄, 中馬 いづみ

    平成25年度日本植物病理学会大会, 2013年, 日本語, 岐阜大学, 国内会議

    口頭発表(一般)

  • ヒストンメチル転移酵素群はイネ科植物いもち病菌の感染において重要な役割を果たす

    Pham Kieu, Vu Ba, Nguyen Quoc, 池田 健一, 井上 喜博, 土佐 幸雄, 中屋敷 均

    日本植物病理学会大会, 2013年, 日本語, 国内会議

    口頭発表(一般)

  • コムギのエンバクいもち病菌・ペレニアルライグラスいもち病菌に対する抵抗性に共通に関与する抵抗性遺伝子—非病原力遺伝子ペアの同定

    Trinh Ty Phung Vy, Vu Lan Anh, Cumagun Christian, 井上 喜博, 中馬 いづみ, 土佐 幸雄

    平成25年度日本植物病理学会大会, 2013年, 英語, 岐阜大学, 国内会議

    口頭発表(一般)

  • いもち病菌非病原力遺伝子AVR-Piztの変異に関わるトランスポゾン挿入と点突然変異

    角 圭人, 中馬 いづみ, 草場 基章, 本藏 良三, 土佐 幸雄

    平成25年度日本植物病理学会大会, 2013年, 日本語, 岐阜大学, 国内会議

    口頭発表(一般)

  • イネいもち病菌のクローナル増殖時に観察されたMAGGY-DNAフィンガープリントパターンの変異

    池上 裕美子, 宮司 貴浩, 鈴木 啓史, 中馬 いづみ, 土佐 幸雄, 草場 基章

    平成25年度日本植物病理学会大会, 2013年, 日本語, 岐阜大学, 国内会議

    口頭発表(一般)

  • 植物病原糸状菌の集団適応戦略-イネいもち病菌を例として

    土佐 幸雄

    第12回糸状菌分子生物学コンファレンスシンポジウム, 2012年11月, 日本語, ウインクあいち, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Screening for candidates of PWT4, a gene for avirulence of an Avena isolate of Magnaporthe oryzae on wheat, using whole-genome sequencing

    Inoue, Y, Yoshida, K, Mitsuoka, C, Asano, H, Terauchi, R, 土佐 幸雄

    IS-MPMI 2012 International Congress., 2012年07月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species.

    中馬 いづみ, C. Isobe, Y. Hotta, K. Ibaragi, N. Futamata, M. Kusaba, K. Yoshida, R. Terauchi, Y. Fujita, 中屋敷 均, B. Valent, 土佐 幸雄

    IS-MPMI 2012 International Congress, 2012年07月, 英語, Kyoto, Japan, 国際会議

    口頭発表(一般)

  • Molecular mapping of Rmo2, a core locus conditioning the resistance of barley to various host-specific subgroups of Magnaporthe oryzae

    Hyon, G-S, N.T.T. Nga, K. Sato, 中馬 いづみ, K. Okada, Y. Inoue, 土佐 幸雄

    IS-MPMI 2012 International Congress, 2012年07月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Identification and molecular mapping of a wheat gene for resistance to a Polypogon isolate of Colletotrichum cereale.

    R. Mori, Y, Inoue, Y, Takahashi, S, Kiguchi, 土佐 幸雄

    IS-MPMI 2012 International Congress, 2012年07月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Genetic analysis of the incompatibility between a Lolium isolate of Magnaporthe oryzae and wheat

    Vy, T.T.P, Y. Inoue, G-S. Hyon, 土佐 幸雄

    IS-MPMI 2012 International Congress, 2012年07月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species

    中馬 いづみ, C. Isobe, Y. Hotta, K. Ibaragi, N. Futamata, M. Kusaba, K. Yoshida, R. Terauchi, Y. Fujita, 中屋敷 均, B. Valent, 土佐 幸雄

    The 2nd Korea-Japan Joint Symposium 2012, 2012年03月, 英語, 国際会議

    ポスター発表

  • Identification and molecular mapping of Rmo2, a core locus conditioning the resistance of barley to various host-specific subgroups of Magnaporthe oryzae.

    Hyon, G-S, N.T.T. Nga, K. Sato, 中馬 いづみ, K. Okada, Y. Inoue, 土佐 幸雄

    The 2nd Korea-Japan Joint Symposium 2012, 2012年03月, 英語, 国際会議

    ポスター発表

  • Genetic analysis of the incompatibility between a Lolium isolate of Magnaporthe oryzae and wheat cultivars

    Trinh, T, Inoue, Y, Hyon, G, 土佐 幸雄

    The 2nd Korea-Japan Joint Symposium 2012, 2012年03月, 英語, 国際会議

    ポスター発表

  • 次世代シーケンサーを用いたエンバクいもち病菌のコムギに対する非病原力遺伝子PWT4の候補遺伝子の探索

    井上喜博, 吉田健太郎, 三岡周子, 麻野北斗, 寺内良平, 土佐 幸雄

    平成24年度日本植物病理学会大会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • コムギいもち病菌のエンバクに対する非病原力遺伝子PAT1のマッピング

    森亮太, 井上喜博, 中馬 いづみ, 土佐 幸雄

    平成24年度糸状菌分子生物学コンファレンス, 2012年, 日本語, 国内会議

    ポスター発表

  • オオムギのいもち病抵抗性遺伝子座Rmo2に対応するアワいもち病菌の非病原力遺伝子PBY3,PBY4の同定と分子マッピング

    中村貴弘, 玄康洙, Nguyen Thi Thanh Nga, 井上喜博, 中馬 いづみ, 土佐 幸雄

    平成24年度日本植物病理学会関西部会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌非病原力遺伝子AVR-Piztの特殊性とそのエフェクター機能の重要性に関する一考察

    角圭人, 中馬 いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成24年度日本植物病理学会大会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌比病原力遺伝子のMultiple translocationはなぜ起こったか―欠失/再獲得仮説

    中馬 いづみ, 古田純一, 草場基章, 吉田健太郎, 寺内良平, 藤田佳克, 中屋敷 均, Barbara Valent, 土佐 幸雄

    平成24年度日本植物病理学会大会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • アワいもち病菌のオオムギに対する非病原力遺伝子PBY3、PBY4の分子マッピング

    中村貴弘, 玄康洙, Nguyen Thi Thanh Nga, 井上喜博, 中馬 いづみ, 土佐 幸雄

    平成24年度植物感染生理談話会, 2012年, 日本語, 国内会議

    ポスター発表

  • Pyricularia属菌集団におけるMBI-D剤耐性遺伝子のゲノム内における存在様式と変異性

    村田暢明, 中馬 いづみ, 鈴木啓史, 草場基章, 土佐 幸雄

    平成24年度植物感染生理談話会, 2012年, 日本語, 国内会議

    ポスター発表

  • MBI-D剤耐性に関与するSDH遺伝子のPyricularia属菌集団における変異性解析

    村田暢明, 中馬 いづみ, 鈴木啓史, 草場基章, 土佐 幸雄

    平成24年度日本植物病理学会関西部会, 2012年, 日本語, 国内会議

    口頭発表(一般)

  • クロロシスを示す合成パンコムギのコムギいもち病菌に対する高い病害抵抗性

    中野 裕樹, 土佐 幸雄, 宅見 薫雄

    日本育種学会, 2011年09月, 日本語, 福井県立大学, 国内会議

    口頭発表(一般)

  • 野生イネ菌、アワ菌、コムギ菌を用いた比較SSRマッピングによるいもち病菌ゲノム構造の解析

    堀田祐麻, 中馬 いづみ, 内藤大貴, 草場基章, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • 非病原力遺伝子AVR-Piztはいもち病菌集団において安定して第7染色体に存在する

    角圭人, 中馬 いづみ, 古田純一, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • 細胞壁加水分解酵素群はイネ科植物いもち病菌による角皮侵入に寄与している

    伊藤賢司, Quoc Bao Nguyen, 土佐 幸雄, 中屋敷 均

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • キビ炭そ病菌に対するコムギの抵抗性遺伝子のマッピング

    高橋裕次郎, 井上喜博, 木口奏, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • オオムギの各種いもち病菌抵抗性に関与する複合遺伝子座Rmo2の同定と分子マッピング

    玄康洙, Nguyen, T.T.N, 中馬 いづみ, 岡田和馬, 佐藤和広, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • オオムギの各種いもち病菌抵抗性に関与する複合遺伝子座Rmo2の作用解析と分子マッピング

    玄康洙, Nguyen, T.T.N, 井上喜博, 中馬 いづみ, 麻野北斗, 佐藤和広, 土佐 幸雄

    平成23年度日本植物病理学会関西部会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT4を認識する新たな抵抗性遺伝子の同定

    井上喜博, 麻野北斗, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌集団Pyricularia spp.における非病原力遺伝子AVR-Pikの染色体彷徨―特に過剰染色体の関与について

    中馬 いづみ, 今井誉子, 古田純一, 角圭人, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌集団Pyricularia spp.における非病原力遺伝子AVR-PiaおよびAVR-Piiの染色体彷徨

    古田純一, 中馬 いづみ, 大窪貴子, 草場基章, 吉田健太郎, 寺内良平, 曾根輝雄, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌の非病原力遺伝子座Avr1-CO39における機能分子の同定

    藤田憲太郎, Hau, V.T.B, Nguyen, Q.B, 中屋敷 均, 土佐 幸雄

    平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • Building Blocks法によるイネ科植物いもち病菌細胞壁加水分解酵素群の機能解析

    伊藤賢司, Quoc Bao Nguyen, 河南裕美, 村部知里, 土佐 幸雄, 中屋敷 均

    平成23年度日本植物病理学会関西部会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • Roles of avirulence genes of Pyricularia species in defining host and nonhost specificity.

    土佐 幸雄

    Plant & Animal Genome conference XIX, 2011年01月, 英語, San Diego, U.S.A., 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • イネ科植物いもち病菌集団における非病原力遺伝子AVR-Pitaの染色体彷徨

    中馬 いづみ, 磯部千尋, 堀田祐麻, 茨木加奈, 二俣翔, 草場基章, 吉田健太郎, 寺内良平, 藤田佳克, 中屋敷 均, Barbara Valent, 土佐 幸雄

    ムギ類研究会, 2010年11月, 日本語, 帯広, 国内会議

    口頭発表(一般)

  • イネ科植物いもち病菌の寄生性分化過程‐コムギ・オオムギをモデルとして

    土佐 幸雄

    ムギ類研究会, 2010年11月, 日本語, 帯広, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • イネいもち病におけるboom-and-bust cycleとマルチライン

    土佐 幸雄

    日本学術会議公開シンポジウム, 2010年11月, 日本語, 日本学術会議講堂, 東京., 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 日本産イネいもち病菌のゲノムに分布するPex31ホモログの数.

    成富穀誌, 馬場崎翔一, 持田泰雅, 今井誉子, 中馬 いづみ, 吉田健太郎, 寺内良平, 土佐 幸雄, 藤田佳克, 草場基章

    平成22年度日本植物病理学会大会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌集団における非病原力遺伝子AVR-Piiファミリーの解析

    古田純一, 中馬 いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成22年度日本植物病理学会関西部会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌の種特異的寄生性に関与する非病原力遺伝子PWT1の染色体座乗領域

    中馬 いづみ, Zhan, S.W, 浅野峻介, Nguyen, N.T.T, Trihn, V.T.P, 白井倫子, 茨木加奈, 土佐 幸雄

    平成22年度日本植物病理学会大会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌サブテロメアにおいて高頻度に検出される遺伝子変換

    中馬 いづみ, 茨木加奈, 土佐 幸雄

    平成22年度日本植物病理学会関西部会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • イネいもち病菌84R-62BとY93-245c-2の子孫菌株で観察されたPex31ホモログの転座

    持田泰雅, 橋川慎吾, 成富穀誌, 中馬 いづみ, 吉田健太郎, 寺内良平, 土佐 幸雄, 藤田佳克, 草場基章

    平成22年度日本植物病理学会大会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • アワいもち病菌のコムギに対する非病原力遺伝子PWT1周辺領域のクローニング

    岡田和馬, 玄 康洙, 白井倫子, セン蘇文, 中馬 いづみ, 土佐 幸雄

    平成22年度日本植物病理学会大会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • RNA silencing as a novel tool for uncovering gene function in fungi

    Nguyen, B.Q, Itoh, K, 土佐 幸雄, 中屋敷 均

    The 4th CeFP International Symposium on Fungal Biology and Pathogenesis, 2010年, 英語, Seoul National University, Korea, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Genetic analyses of “host species specificity”of Magnaporthe oryzae/grisea.

    土佐 幸雄, 中馬 いづみ

    10th US-JAPAN Cooperative Scientific Seminar, 2010年01月, 英語, Corvallis, Oregon, USA, 国際会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 分泌タンパク質遺伝子のマッピングによるいもち病菌染色体高度可変領域の同定

    浅野峻介, 中馬 いづみ, 古田純一, 今井誉子, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成21年度日本植物病理学会関西部会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 植物病原糸状菌における種特異的寄生性の遺伝学的解析

    土佐 幸雄

    平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 冠さび病菌感染エンバク葉に誘導されるアベナスラミド蓄積とその生合成遺伝子発現の局在と配向解析

    内橋幸平, 岡村薫, 石原亨, 朴 杓允, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • さまざまないもち病菌菌株における非病原力遺伝子AVR-Pii の周辺構造

    古田純一, 中馬 いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成21年度日本植物病理学会関西部会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • コムギのメヒシバいもち病菌に対する抵抗性遺伝子の同定

    Nga, N.T.T, Hau, V.T, B, 土佐 幸雄

    平成21年度日本植物病理学会関西部会, 2009年, 英語, 国内会議

    口頭発表(一般)

  • エンバクいもち病菌のコムギに対する非病原力遺伝子PWT4へのクロモソームウオーキング

    井上喜博, Hau, V.T, B, 中馬 いづみ, 土佐 幸雄

    平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌小分生子の細胞学的特徴

    中馬 いづみ, 中屋敷 均, 土佐 幸雄

    平成21年度日本植物病理学会関西部会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌集団における非病原力遺伝子AVR-Pii の分布

    古田純一, 中馬 いづみ, 草場基章, 吉田健太郎, 寺内良平, 土佐 幸雄

    平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌における非病原力遺伝子の比較解析とその進化過程に関する考察

    中馬 いづみ, 草場基章, 大窪貴子, 古田純一, 萬野英俊, 吉田健太郎, 寺内良平, 曾根輝雄, 土佐 幸雄

    平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • Super-SAGE法を用いたエンバクいもち病菌のコムギに対する非病原力遺伝子PWT4の候補遺伝子の選抜

    井上喜博, 麻野北斗, 中馬 いづみ, 吉田健太郎, 松村英生, 寺内良平, 土佐 幸雄

    平成21年度日本植物病理学会関西部会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • Setaria 属寄生性いもち病菌におけるAVR-Pitaの分布

    萬野英俊, 中馬 いづみ, 祝千尋, 諸石雅彦, 草場基章, 土佐 幸雄

    平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌小型分生子の病理学的役割を解明するための基礎調査

    細木 直樹, 中馬 いづみ, 篠木 武, 池田 健一, 中屋敷 均, 眞山 滋志, 土佐 幸雄, 朴 杓允

    日本植物病理学会, 2007年, 日本語, 国内会議

    口頭発表(一般)

  • いもち病菌のシトシンDNAメチル化は同菌の生活環において必須ではない

    池田 健一, 田中 正起, 村田 聡樹, 椎名 宏太, 土佐 幸雄, 眞山 滋志, 朴 杓允, 中屋敷 均

    日本植物病理学会関西部会, 2007年, 日本語, 国内会議

    口頭発表(一般)

  • 比較マッピングによるいもち病菌の染色体構造解析

    茨木 加奈, 中馬 いずみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成17年日本植物病理学会関西部会講演要旨集p29, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議

    口頭発表(一般)

  • 植物病原糸状菌Magnaporthe oryzaeにおけるRNAi経路には二つのDicer 様タンパク質のうち一つが主に関与する

    角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成17年日本植物病理学会大会 (日本植物病理学会報,71: 199), 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議

    口頭発表(一般)

  • シコクビエいもち病菌集団における非病原力遺伝子PWL1 の解析

    田中 正起, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成17年日本植物病理学会大会 (日本植物病理学会報,71: 198-199), 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議

    口頭発表(一般)

  • コムギのエンバクいもち病菌に対する抵抗性遺伝子Rwt4の野外における役割

    Hau V.T.B, 平田 健治, 村上 二朗, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成17年日本植物病理学会大会 (日本植物病理学会報,71: 199), 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議

    口頭発表(一般)

  • いもち病菌集団における非病原力遺伝子Avr-Pita 座の変動機構

    中馬 いずみ, 磯部 千尋, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成17年日本植物病理学会大会 (日本植物病理学会報,71: 198), 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議

    口頭発表(一般)

  • いもち病菌の種特異的寄生性に関するPWT3 に対応する抵抗性遺伝子の検出とgene-forgene関係の証明

    田澤 佳子, 平田 健治, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成17年日本植物病理学会関西部会講演要旨集p30, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議

    口頭発表(一般)

  • イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定

    椎名 宏太, 池田 健一, 角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成17年日本植物病理学会関西部会講演要旨集p30, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議

    口頭発表(一般)

  • イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定

    椎名宏太, 池田 健一, 角谷直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    日本植物病理学会関西部会, 2005年, 日本語, 国内会議

    口頭発表(一般)

  • アワいもち病菌のイチゴツナギ亜科植物に対する非病原性の比較解析

    大村 和孝, 田中 健吾, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成17年日本植物病理学会関西部会講演要旨集p29, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議

    口頭発表(一般)

  • RNA silencing as a tool for exploring gene function in Ascomycete fungi.

    NAKAYASHIKI Hitoshi, HANADA S, QUOC N.B, KADOTANI N, TOSA Yukio, MAYAMA Shigeyuki

    XXIII Fungal Genetics Conference. Abstact p163., 2005年, 英語, 未記入, California, 国際会議

    その他

  • Pyricularia oryzae 近縁種の種分化過程のGCPSRによる解析

    平田 健治, 草場 基章, 中馬 いずみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成17年日本植物病理学会大会 (日本植物病理学会報,71: 198), 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議

    口頭発表(一般)

  • One of the two Dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related siRNA accumulation.

    KADOTANI N, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    XXIII Fungal Genetics Conference. Abstact p159., 2005年, 英語, 未記入, California, 国際会議

    その他

  • DNA methyltransferase は転移因子由来siRNAの蓄積を促進する

    角谷 直樹, 椎名 宏太, 池田 健一, 土佐 幸雄, 眞山 滋志, 中屋敷 均

    第7回RNA学会ミーティング(講演要旨集p86), 2005年, 日本語, 日本RNA学会, 弘前大学, 国内会議

    口頭発表(一般)

  • DNA methyltransferaseは転移因子由来siRNAの蓄積を促進する

    角谷 直樹, 椎名 宏太, 池田 健一, 土佐 幸雄, 眞山 滋志, 中屋敷 均

    第7回RNA学会, 2005年, 日本語, 弘前大学, 国内会議

    口頭発表(一般)

  • 農薬vs植物の化学兵器

    土佐 幸雄

    第3回神戸大学農学部秋季公開セミナー -農学が築く食の安全と安心-, 2004年11月, 日本語, 神戸大学農学部, 兵庫県私学会館, 国内会議

    口頭発表(一般)

  • 様々な植物から分離したPyricularia属菌におけるGCPSRに基づいた種の識別

    平田 健治, 大末 淳, 中馬 いずみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • 植物病原糸状菌Magnaporthe oryzaeにおけるRNAi経路には二つのDicer様タンパク質のうち一つが主に関与する

    角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    第6回RNAミーティング, 2004年, 日本語, RNAミーティング, 国内会議

    口頭発表(一般)

  • トランスポゾン配列の分布およびITS領域の塩基配列に基づくイタリアンライグラスいもち病菌の分類学的特徴

    山頭 亜紀子, 浦田 秀美, 平田 健治, 角田 佳則, 土佐 幸雄, 眞山 滋志, 草場 基章, 八重樫 博志

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • セントピートグラスに発生したいもち病菌のrDNA-ITS2領域とbeta-tubulin遺伝子の解析における病原性発現物質の解析

    冨家 和典, 森 真理, 中屋敷 均, 土佐 幸雄, 松浦 一穂, 眞山 滋志, 但見 明俊

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • シコクビエいもち病菌(Magnaporthe oryzae)の集団構造の解析

    田中 正起, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成16年日本植物病理学会関西部会, 2004年, 日本語, 日本植物病理学会, 松山, 国内会議

    口頭発表(一般)

  • コムギ品種とエンバクいもち病菌間における遺伝子対遺伝子関係の証明

    平田 健治, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成16年日本植物病理学会関西部会, 2004年, 日本語, 日本植物病理学会, 松山, 国内会議

    口頭発表(一般)

  • エンバクの抵抗性誘導におけるアポトーシス機構の解明14)エンバクにおける宿主特異的毒素ビクトリンへの結合因子のビアコアによる探索

    齋藤 隆一郎, 日下 広, 劉 小露, 中屋敷 均, 土佐 幸雄, 眞山 滋志, 多田 安臣

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • エンバクの抵抗性誘導におけるアポトーシス機構の解明13)宿主特異的毒素ビクトリンは感受性品種IowaX469において細胞表面で認識される

    多田 安臣, 日下 広, 松原 孝英, 大浦 康子, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • いもち病菌集団における染色体構造の多様性と非病原性遺伝子座の変動

    中馬 いずみ, 磯部 千尋, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • いもち病菌Magnaporthe oryzaeにおけるRNAサイレンシングベクターの作製

    花田 周吾, 角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • Degradation of ribosomal RNA associated with apoptotic response of oat cells trigered by the host-selective toxin, victorin.

    HOAT T X, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    平成16年日本植物病理学会関西部会, 2004年, 英語, 日本植物病理学会, 松山, 国内会議

    口頭発表(一般)

  • Arabidopsisにおける植物成長促進根圏微生物(PGPR)の遺伝子変動のマイクロアレイ解析

    大原 裕美子, 王 艶青, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議

    口頭発表(一般)

  • A convergent opposing promoters system to explore genome-wide gene function in the rice blast fungus by an RNA silencing approach.

    QUOC N B, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

    平成16年日本植物病理学会関西部会, 2004年, 英語, 日本植物病理学会, 松山, 国内会議

    口頭発表(一般)

  • いもち病菌Magnaporthe oryzae におけるRNA サイレンシング

    角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    第3回糸状菌分子生物学コンファレンス, 講演要旨集, 2003年11月, 日本語, 糸状菌分子生物学研究会, 東京大学, 国内会議

    口頭発表(一般)

  • Pyricularia 属集団における染色体構造の多様性

    中馬 いづみ, 磯部 千尋, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    第3回糸状菌分子生物学コンファレンス, 講演要旨集, 2003年11月, 日本語, 糸状菌分子生物学研究会, 東京大学, 国内会議

    口頭発表(一般)

  • いもち病菌における過剰染色体の遺伝様式と染色体構造変異の機構

    中馬 いづみ, 土佐 幸雄, 多賀 正節, 中屋敷 均, 眞山 滋志

    第75回日本遺伝大会, 講演要旨集, 2003年09月, 日本語, 日本遺伝大会, 東北大学, 国内会議

    口頭発表(一般)

  • ペレニアルライグラスいもち病菌の地理的機嫌の解明とコムギに対する発病ポテンシャルの評価

    中川 志織, 土佐 幸雄, 平田 健治, 中馬 いづみ, 磯部 千尋, 中屋敷 均, 森 直樹, WAKAR Uddin, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • ナス科植物青枯病生物防除因子Pseudomonas fluorescens のNOR遺伝子破壊による改変

    王 艶青, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • いもち病菌集団における染色体構造の多様性

    磯部 千尋, 中馬 いづみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • いもち病菌の種特異的寄生性機構に関する研究(13) いもち病菌雑種後代で認められるadult resistance に対する病原性の低下について

    村上 二朗, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • いもち病菌における病原性発現物質の解析7)エンバクいもち病菌の生産する新規毒素の白化,活性酸素生成, 細胞死の誘導

    南 友可里, 鶴嶋 鉄, 坂本 勝, 宮川 恒, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • いもち病菌におけるsupernumerary chromosome の遺伝様式と構造変異機構

    中馬 いづみ, 土佐 幸雄, 多賀 正節, 中屋敷 均, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • GCPSR に基づいたMagnaporthe/Pyricularia 属菌集団における種の識別

    平田 健治, 大末 淳, 中馬 いづみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • いもち病菌Magnaporthe grisea におけるsmall interfering RNAs (siRNAs)の検出

    角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年, 日本語, 日本植物病理学会, 未記入, 国内会議

    口頭発表(一般)

  • Mechanisms of species-specific parasitism in Magnaporthe oryzae.

    TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

    Plant & Animal Genome XI Conference., 2003年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Apoptotic response of oat leaf cells in defense against infection.

    MAYAMA Shigeyuki, NAN Y, KUSAKA K, TADA Y, YANG Q, SHIGEMI T, IMAI S, NAKAYASHIKI Hitoshi, TOSA Yukio, PARK P

    9th US-Japan Seminar on Plant-pathogen Interactions., 2003年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

所属学協会

  • Americal Phytopathological Society

  • Phytopathological Society of Japan

Works(作品等)

  • Roles of the AVR1-CO39 avirulence gene in the specific parasitism of Magnaporthe

    1996年

共同研究・競争的資金等の研究課題

産業財産権

  • キチン質分解酵素を遺伝子標識に利用した細菌モニタリング法

    土佐 幸雄, 眞山 滋志, 大津 康成, 豊田 秀吉, 桜谷 保之, 松田 克礼, 野々村 照雄, 瀧川 義浩, 森 裕文

    特願2002-2690418, 2002年09月05日, 大学長, 特許3726132, 2005年10月07日

    特許権

  • 食葉性害虫に対し抑制効果を有する細菌を内包したアルギン酸マイクロビーズによる害虫駆除の方法

    土佐 幸雄, 眞山 滋志, 大津 康成, 豊田 秀吉, 桜谷 保之, 松田 克礼, 野々村 照雄, 瀧川 義浩, 森 裕文

    特願2002-260419, 2002年09月05日, 大学長, 特許3686945, 2005年06月17日

    特許権