研究者紹介システム

荻野 千秋
オギノ チアキ
大学院工学研究科 応用化学専攻
教授
応用化学関係
Last Updated :2022/06/23

研究者情報

所属

  • 【主配置】

    大学院工学研究科 応用化学専攻
  • 【配置】

    学術研究推進機構, 工学部 応用化学科, 先端バイオ工学研究センター

学位

  • 博士(工学), 神戸大学
  • 微生物の力によるバイオマスからのバイオ燃料や化学品生産

授業科目

ジャンル

  • 科学・技術 / バイオテクノロジー

コメントテーマ

  • バイオマス
  • バイオリファイナリー
  • 酸化チタンナノ粒子
  • 放射線治療

研究ニュース

研究活動

研究分野

  • ナノテク・材料 / ナノバイオサイエンス
  • ものづくり技術(機械・電気電子・化学工学) / バイオ機能応用、バイオプロセス工学

受賞

  • 2021年10月 神戸大学, 第13回学長表彰(財務貢献者)

    荻野 千秋

  • 2019年10月 神戸大学, 第11回学長表彰(財務貢献者)

    荻野 千秋

  • 2018年10月 神戸大学, 第10回学長表彰(財務貢献者)

    荻野 千秋

  • 2017年10月 神戸大学, 第9回学長表彰(財務貢献者)

    荻野 千秋

  • 2013年09月 日本生物工学会, 生物工学論文賞, 合成代謝経路と細胞表層工学を利用した組換え大腸菌によるセロビオースからのイソプロパノール生産

    相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 岡本 正宏, 花井 泰三

    日本国

    国内学会・会議・シンポジウム等の賞

  • 2007年03月 化学工学会, 平成18年度化学工学会 奨励賞(實吉雅郎記念賞), リン脂質修飾酵素ホスホリパーゼDに関する基礎解析および生産技術の開発

    荻野 千秋

論文

  • Hideo Kawaguchi, Kenji Takada, Taghreed Elkasaby, Radityo Pangestu, Masakazu Toyoshima, Prihardi Kahar, Chiaki Ogino, Tatsuo Kaneko, Akihiko Kondo

    Elsevier BV, 2022年01月, Bioresource Technology, 344, 126165 - 126165, 英語

    研究論文(学術雑誌)

  • Prihardi Kahar, Nova Rachmadona, Radityo Pangestu, Rendi Palar, Deddy Triyono Nugroho Adi, Ario Betha Juanssilfero, Yopi, Immanuel Manurung, Shinji Hama, Chiaki Ogino

    Elsevier BV, 2022年01月, Bioresource Technology, 344, 126266 - 126266, 英語

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Tomohisa Hasunuma, Yasuo Ohnishi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino

    Abstract Background Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics. Results Under aerobic conditions, a recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI genes derived from Streptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. An ldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture. Conclusions Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives. Graphical Abstract

    Springer Science and Business Media LLC, 2021年12月, Microbial Cell Factories, 20 (1), 228, 英語

    研究論文(学術雑誌)

  • Taku Sakuragawa, Satoshi Wakai, Silai Zhang, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

    Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.

    Elsevier BV, 2021年08月, Journal of Bioscience and Bioengineering, 132 (2), 140 - 147, 英語, 国内誌

    研究論文(学術雑誌)

  • Nova Rachmadona, Emmanuel Quayson, Jerome Amoah, Daniel A Alfaro‐Sayes, Shinji Hama, Martha Aznury, Akihiko Kondo, Chiaki Ogino

    Wiley, 2021年05月, Biofuels, Bioproducts and Biorefining, 15 (3), 804 - 814, 英語

    研究論文(学術雑誌)

  • Kenta Morita, Yuya Nishimura, Satoko Nakamura, Yuki Arai, Chiya Numako, Kazuyoshi Sato, Masao Nakayama, Hiroaki Akasaka, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

    Elsevier BV, 2021年02月, Colloids and Surfaces B: Biointerfaces, 198, 111451 - 111451

    研究論文(学術雑誌)

  • Aniruddha Nag, Mohammad Asif Ali, Hideo Kawaguchi, Shun Saito, Yukie Kawasaki, Shoko Miyazaki, Hirotoshi Kawamoto, Deddy Triyono Nugroho Adi, Kumiko Yoshihara, Shunsuke Masuo, Yohei Katsuyama, Akihiko Kondo, Chiaki Ogino, Naoki Takaya, Tatsuo Kaneko, Yasuo Ohnishi

    Wiley, 2021年01月, Advanced Sustainable Systems, 5 (1), 2000193 - 2000193

    研究論文(学術雑誌)

  • Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

    Microbiology Research Foundation, 2021年, The Journal of General and Applied Microbiology, 67 (6), 265 - 268, 英語

    研究論文(学術雑誌)

  • Emmanuel Quayson, Jerome Amoah, Shinji Hama, Akihiko Kondo, Chiaki Ogino

    Elsevier BV, 2020年12月, Renewable and Sustainable Energy Reviews, 134, 110355 - 110355

    研究論文(学術雑誌)

  • Yuya Nishimura, Ryosuke Ezawa, Kenta Morita, Masao Nakayama, Jun Ishii, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

    American Chemical Society (ACS), 2020年11月16日, ACS Applied Bio Materials, 3 (11), 7743 - 7751

    研究論文(学術雑誌)

  • Emmanuel Quayson, Jerome Amoah, Nova Rachmadona, Kenta Morita, Lawrence Darkwah, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

    Continuous expansion of agriculture for the production of biofuels may be considered a potential source of greenhouse gas (GHG) emissions due to the ever-increasing amount of waste and fossil fuel-dependent materials involved. Agricultural waste utilization through the circular bioeconomy concept offers a pathway to reduce GHG emissions. Palm oil production, for instance, produces palm kernel shells (PKS) and palm oil mill effluents (POME) as wastes in enormous amounts. PKS and POME account for >60% of solid and liquid waste generated from the mill. In this work, the feasibility of a circular palm bioeconomy is explored where waste PKS is directly converted to activated carbons (AC) in a cost-effective one-step technique (550 degrees C, 10 mL min(-1) N-2) that departs from the conventional two-step (carbonization and activation) technique. Characterization of the synthesized carbons, PKAC, using Fourier-transform infrared spectroscopy and scanning electron microscopy with energy-dispersive X-ray spectroscopy showed oxygen-rich morphological features that were 2-fold higher than in bituminous coal-derived AC. The <10 mu m pore diameters of PKAC were relevant in the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase (PKAC-FHL). For biodiesel production, the 81.6 IU mg(-1) specific activity of PKAC-FHL yielded >= 97.5 wt% fatty acid methyl ester (FAME) from POME. While offering the benefits of environmental remediation through effluent utilization, the produced FAME showed 48.8 Cetane Number and cold-flow properties that conform to ASTM and EN biodiesel requirements. This waste valorization approach, thus, proposes a green framework for the use of POME and PKS in immobilized lipase-catalyzed methanolysis.

    Elsevier BV, 2020年11月, Biomass and Bioenergy, 142, 105768 - 105768, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Silai Zhang, Satoshi Wakai, Naoya Sasakura, Hiroko Tsutsumi, Yoji Hata, Chiaki Ogino, Akihiko Kondo

    Pyruvate is a central metabolite for the biological production of various chemicals. In eukaryotes, pyruvate produced by glycolysis is used in conversion to ethanol and lactate and in anabolic metabolism in the cytosol, or is transported into the mitochondria for use as a substrate in the tricarboxylic acid (TCA) cycle. In this study, we focused on controlling pyruvate metabolism in aerobic microorganisms for the biological production of various chemicals. We successfully improved productivity by redirecting pyruvate metabolism in the aerobic filamentous fungus Aspergillus oryzae via the deletion of two genes that encode pyruvate decarboxylase and mitochondrial pyruvate carriers. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Although the dry mycelial weights of the deletion mutants were reduced compared with those of wild type, the titer and yields of the target products were drastically increased. In particular, the redirection of pyruvate metabolism shifted from anabolism for biomass production to catabolism for the production of target chemicals. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms.

    2020年07月02日, Metabolic engineering, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Mennaallah Hassan, Masao Nakayama, Mohammed Salah, Hiroaki Akasaka, Hikaru Kubota, Makiko Nakahana, Tatsuichiro Tagawa, Kenta Morita, Ai Nakaoka, Takeaki Ishihara, Daisuke Miyawaki, Kenji Yoshida, Yuya Nishimura, Chiaki Ogino, Ryohei Sasaki

    The development of potentially safe radiosensitizing agents is essential to enhance the treatment outcomes of radioresistant cancers. The titanium peroxide nanoparticle (TiOxNP) was originally produced using the titanium dioxide nanoparticle, and it showed excellent reactive oxygen species (ROS) generation in response to ionizing radiation. Surface coating the TiOxNPs with polyacrylic acid (PAA) showed low toxicity to the living body and excellent radiosensitizing effect on cancer cells. Herein, we evaluated the mechanism of radiosensitization by PAA-TiOxNPs in comparison with gold nanoparticles (AuNPs) which represent high-atomic-number nanoparticles that show a radiosensitizing effect through the emission of secondary electrons. The anticancer effects of both nanoparticles were compared by induction of apoptosis, colony-forming assay, and the inhibition of tumor growth. PAA-TiOxNPs showed a significantly more radiosensitizing effect than that of AuNPs. A comparison of the types and amounts of ROS generated showed that hydrogen peroxide generation by PAA-TiOxNPs was the major factor that contributed to the nanoparticle radiosensitization. Importantly, PAA-TiOxNPs were generally nontoxic to healthy mice and caused no histological abnormalities in the liver, kidney, lung, and heart tissues.

    2020年06月07日, Nanomaterials (Basel, Switzerland), 10 (6), 英語, 国際誌

    研究論文(学術雑誌)

  • High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration

    Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Nanik Rahmani, Euis Hermiati, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    2020年05月, Processes, 8, 619, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

    Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Emmanuel Quayson, Nova Rachmadona, Jerome Amoah, Shinji Hama, Chiaki Ogino, Akihiko Kondo

    2020年04月, Processes, 8 (4), 450, 英語

    [査読有り]

  • Naoki Watanabe, Masahiro Murata, Teppei Ogawa, Christopher J Vavricka, Akihiko Kondo, Chiaki Ogino, Michihiro Araki

    Unannotated gene sequences in databases are increasing due to sequencing advances. Therefore, computational methods to predict functions of unannotated genes are needed. Moreover, novel enzyme discovery for metabolic engineering applications further encourages annotation of sequences. Here, enzyme functions are predicted using two general approaches, each including several machine learning algorithms. First, Enzyme-models (E-models) predict Enzyme Commission (EC) numbers from amino acid sequence information. Second, Substrate-Enzyme models (SE-models) are built to predict substrates of enzymatic reactions together with EC numbers, and Substrate-Enzyme-Product models (SEP-models) are built to predict substrates, products, and EC numbers. While accuracy of E-models is not optimal, SE-models and SEP-models predict EC numbers and reactions with high accuracy using all tested machine learning-based methods. For example, a single Random Forests-based SEP-model predicts EC first digits with an Average AUC score of over 0.94. Various metrics indicate that the current strategy of combining sequence and chemical structure information is effective at improving enzyme reaction prediction.

    2020年03月23日, Journal of chemical information and modeling, 60 (3), 1833 - 1843, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Nova Rachmadona, Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

    Palm oil mill effluent (POME), a liquid waste from the palm oil industry, presents an alternative source for biodiesel production without interfering with food supply. This study attempted to produce biodiesel from untreated POME with aqueous ethanol using Thermomyces lanuginosus lipase as a biocatalyst. The effects of enzyme concentration, alcohol to oil ratio, and ethanol concentration were considered in the transesterification reaction. The optimum conditions were 2100 U lipase loading, 4 : 1 ethanol to oil molar ratio, and 45% (v/v) ethanol concentration at 40 degrees C reaction, and under 24 hours. The maximum fatty acid ethyl ester (FAEE) yield reached 97.43% (w/w) under these conditions. Integration of dilute ethanol for the conversion of POME to biodiesel could be promising as both feedstocks could be obtained from the same location, and thus reduce the logistical burden on biodiesel production.

    ROYAL SOC CHEMISTRY, 2020年03月, SUSTAINABLE ENERGY & FUELS, 4 (3), 1105 - 1111, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Biodiesel-mediated biodiesel production: A recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils

    Emmanuel Quayson, Jerome Amoah, Nova Rachmadona, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

    2020年03月, Fuel Processing Technology, 199, 106278, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Apurav Krishna Koyande, Pau-Loke Show, Ruixin Guo, Bencan Tang, Chiaki Ogino, Jo-Shu Chang

    Microalgae biomass contains various useful bio-active components. Microalgae derived biodiesel has been researched for almost two decades. However, sole biodiesel extraction from microalgae is time-consuming and is not economically feasible due to competitive fossil fuel prices. Microalgae also contains proteins and carbohydrates in abundance. Microalgae are likewise utilized to extract high-value products such as pigments, anti-oxidants and long-chain polyunsaturated fatty acids which are useful in cosmetic, pharmaceutical and nutraceutical industry. These compounds can be extracted simultaneously or sequentially after biodiesel extraction to reduce the total expenditure involved in the process. This approach of bio-refinery is necessary to promote microalgae in the commercial market. Researchers have been keen on utilizing the bio-refinery approach to exploit the valuable components encased by microalgae. Apart from all the beneficial components housed by microalgae, they also help in reducing the anthropogenic CO2 levels of the atmosphere while utilizing saline or wastewater. These benefits enable microalgae as a potential source for bio-refinery approach. Although life-cycle analysis and economic assessment do not favor the use of microalgae biomass feedstock to produce biofuel and co-products with the existing techniques, this review still aims to highlight the beneficial components of microalgae and their importance to humans. In addition, this article also focuses on current and future aspects of improving the feasibility of bio-processing for microalgae bio-refinery.

    2019年12月, Bioengineered, 10 (1), 574 - 592, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Amza Rezky Lastinov, Kahar Prihardi, Juanssilfero Ario Betha, Miyamoto Nao, Otsuka Hiromi, Kihira Chie, Ogino Chiaki, Kondo Akihiko

    2019年09月15日, BIOCHEMICAL ENGINEERING JOURNAL, 149

    [査読有り]

  • Amoah Jerome, Ogura Kazuma, Schmetz Quentin, Kondo Akihiko, Ogino Chiaki

    2019年09月, BIOMASS & BIOENERGY, 128

    [査読有り]

  • Guirimand GGY, Bamba T, Matsuda M, Inokuma K, Morita K, Kitada Y, Kobayashi Y, Yukawa T, Sasaki K, Ogino C, Hasunuma T, Kondo A

    Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro-organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae (S. cerevisiae) (YPH499-SsXR-AaBGL) expressing cytosolic xylose reductase (Scheffersomyces stipitis xylose reductase [SsXR]), along with a β-d-glucosidase (Aspergillus aculeatus β-glucosidase 1 [AaBGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5-fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XR-ScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae.

    2019年09月, Biotechnology journal, 14 (9), e1800704, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Nathan Hillson, Mark Caddick, Yizhi Cai, Jose A Carrasco, Matthew Wook Chang, Natalie C Curach, David J Bell, Rosalind Le Feuvre, Douglas C Friedman, Xiongfei Fu, Nicholas D Gold, Markus J Herrgård, Maciej B Holowko, James R Johnson, Richard A Johnson, Jay D Keasling, Richard I Kitney, Akihiko Kondo, Chenli Liu, Vincent J J Martin, Filippo Menolascina, Chiaki Ogino, Nicola J Patron, Marilene Pavan, Chueh Loo Poh, Isak S Pretorius, Susan J Rosser, Nigel S Scrutton, Marko Storch, Hille Tekotte, Evelyn Travnik, Claudia E Vickers, Wen Shan Yew, Yingjin Yuan, Huimin Zhao, Paul S Freemont

    The original version of this Comment contained errors in the legend of Figure 2, in which the locations of the fifteenth and sixteenth GBA members were incorrectly given as '(15) Australian Genome Foundry, Macquarie University; (16) Australian Foundry for Advanced Biomanufacturing, University of Queensland.'. The correct version replaces this with '(15) Australian Foundry for Advanced Biomanufacturing (AusFAB), University of Queensland and (16) Australian Genome Foundry, Macquarie University'. This has been corrected in both the PDF and HTML versions of the Comment.

    2019年07月11日, Nature communications, 10 (1), 3132 - 3132, 英語, 国際誌

    [査読有り]

  • Quentin Schmetz, Hiroshi Teramura, Kenta Morita, Tomoko Oshima, Aurore Richel, Chiaki Ogino, Akihiko Kondo

    © 2019 American Chemical Society. An organosolv pretreatment consisting of an H2SO4/n-butanol biphasic system was designed to separate lignocellulosic biomass in three distinct phases: a cellulose-rich solid residue, hydrolyzed hemicelluloses in an aqueous phase, and lignin dissolved in a hydrophobic butanol phase. In the present study, the versatility of the process was investigated on materials of various compositions and origins: sugar cane bagasse, tall fescue, sugar beet pulp, eucalyptus, beech, and Japanese cedar. The efficiency was assessed in terms of lignin removal from the raw biomass and purity of the recovered cellulosic residue using the Klason method as well as improvement on enzymatic saccharification (increased from 18.7% to 96%). Results were correlated to biomass types and composition, and in comparison to an organic solvent-free method (dilute acid). Up to 81% cellulose purity corresponding to 87% lignin removal was achieved. Results were corroborated by scanning electron microscopy showing an absence of lignin deposition. Lignin molecular weight (GPC), structure (2D-HSQC NMR), recovery, and purity (up to 96%) have been investigated. Moreover, organic compounds responsible for fermentation inhibition were partially solubilized in the butanol, decreasing the concentration in the aqueous phase. Efficient butanol pretreatment applied on hardwood, bagasse, and herbaceous matter is promising. However, Japanese cedar (softwood) was too recalcitrant for this process.

    2019年07月01日, ACS Sustainable Chemistry and Engineering, 7 (13), 11069 - 11079

    [査読有り]

    研究論文(学術雑誌)

  • Yew Guo Yong, Thing Chai Tham, Law Chung Lim, Dinh-Toi Chu, Ogino Chiaki, Show Pau Loke

    2019年06月, MATERIALS TODAY COMMUNICATIONS, 19, 39 - 50

    [査読有り]

  • Hideo Kawaguchi, Hiroki Miyagawa, Sachiko Nakamura-Tsuruta, Naoki Takaya, Chiaki Ogino, Akihiko Kondo

    3-Phenyllactic acid (PhLA) is useful as a start-up material in the pharmaceutical and biorefinery industries. To enhance the production of PhLA from glucose using recombinant Escherichia coli, the effects of glucose concentration and oxygen limitation on PhLA production are assessed in a fed-batch system using dissolved oxygen (DO)-stat method. The highest titer of PhLA (7.3 g L-1 ) is observed with a high concentration of glucose and under oxygen-limited conditions (DO = 0 ppm). Under oxygen limitation, cell growth and the formation of acetate and l-phenylalanine (Phe) by-products after 72 h of cultivation are reduced by 30%, 70%, and 81%, respectively, as compared to that under high DO conditions (DO = 5 ppm). Gene expression levels are compared between low and high DO conditions by quantitative polymerase chain reaction (qPCR) analysis. Several genes in the glycolysis (gapA and pykA), pentose phosphate (tktA), and early shikimate pathways for PhLA biosynthesis (aroF, aroG, and aroH) are upregulated under oxygen limitation. The results suggest that oxygen limitation affects metabolism in the shikimate pathway at both metabolic and transcriptional levels and that controlling the DO level is critical for enhanced production of a variety of aromatic compounds through the shikimate pathway.

    2019年06月, Biotechnology journal, 14 (6), e1800478, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Jerome Amoah, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

    Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have, however, been the bottleneck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Over the last decade, research has shown possibilities of generating tens of new products but only few industrial implementations. This is partly associated with low production yields and poor cost-competitiveness. This review addresses the technical barriers associated with the conversion of emerging feedstocks into chemicals and bioenergy platforms and summarizes the developed biotechnological approaches including advances in metabolic engineering. This summary further suggests possible future advances that would expand the portfolio of biorefinery and speed up the realization of biofuels and biochemicals.

    2019年06月, Biotechnology journal, 14 (6), e1800494, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Yopi, Kumar Sudesh, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.

    2019年06月, Journal of bioscience and bioengineering, 127 (6), 726 - 731, 英語, 国内誌

    [査読有り]

    研究論文(学術雑誌)

  • Nathan Hillson, Mark Caddick, Yizhi Cai, Jose A Carrasco, Matthew Wook Chang, Natalie C Curach, David J Bell, Rosalind Le Feuvre, Douglas C Friedman, Xiongfei Fu, Nicholas D Gold, Markus J Herrgård, Maciej B Holowko, James R Johnson, Richard A Johnson, Jay D Keasling, Richard I Kitney, Akihiko Kondo, Chenli Liu, Vincent J J Martin, Filippo Menolascina, Chiaki Ogino, Nicola J Patron, Marilene Pavan, Chueh Loo Poh, Isak S Pretorius, Susan J Rosser, Nigel S Scrutton, Marko Storch, Hille Tekotte, Evelyn Travnik, Claudia E Vickers, Wen Shan Yew, Yingjin Yuan, Huimin Zhao, Paul S Freemont

    2019年05月09日, Nature communications, 10 (1), 2040 - 2040, 英語, 国際誌

    研究論文(学術雑誌)

  • Guirimand Gregory, Inokuma Kentaro, Bamba Takahiro, Matsuda Mami, Morita Kenta, Sasaki Kengo, Ogino Chiaki, Berrin Jean-Guy, Hasunuma Tomohisa, Kondo Akihiko

    Xylitol is a major commodity chemical widely used in both the food and pharmaceutical industries. Although the worldwide demand for xylitol is constantly growing, its industrial production from purified D-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production of xylitol from biomass is a promising strategy to establish an environmentally friendly sustainable conversion process. In this study, xylitol was produced from woody Kraft pulp (KP) by using an engineered strain of Saccharomyces cerevisiae (YPH499-XR-BGL-XYL-XYN) expressing cytosolic xylose reductase (XR), along with beta-D-glucosidase (BGL), xylosidase (XYL) and xylanase (XYN) enzymes co-displayed on the cell surface. All these enzymes contributed to the consolidated bioprocessing of KP to xylitol with a yield of 2.3 g L-1 (28% conversion) after 96 hours, along with a significantly reduced amount of commercial enzymes required for pre-treatment (commercial hemicellulase cocktail (CHC), [CHC] = 0.02 g-DW per g). Further improvement of the cell surface display of XYL and XYN was obtained by using a SED1 "SSS" cassette, containing the coding sequences of the SED1 promoter, the SED1 secretion signal, and the SED1 anchoring domain, to generate the improved strain YPH499-XR-BGL-XYLsss-XYNsss. This improved strain showed a significantly enhanced xylitol production capacity reaching a yield of 3.7 g L-1 (44% conversion) after 96 hours. The cellulosic content of KP residues was also significantly increased, from 78% to 87% after 96 hours of fermentation, and nanofibrillation of KP residues was observed by scanning electron microscopy. Pre-treatment and fermentation were successfully performed as a proof of concept to further scale up bio-refinery industrial production of xylitol from lignocellulose.

    ROYAL SOC CHEMISTRY, 2019年04月07日, GREEN CHEMISTRY, 21 (7), 1795 - 1808, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Quayson Emmanuel, Amoah Jerome, Hama Shinji, Yoshida Ayumi, Morita Kenta, Kondo Akihiko, Ogino Chiaki

    The biomass-derived source, low-cost and hydrophobicity/oleophilic advantages of activated carbon (AC) were explored for the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase. The adsorptive influence of AC favored growth of the cells into its porous interfaces with paralleled exterior dense film formation. Increasing AC weights hindered extracellular lipase activity. Cell aggregation of 0.34 +/- 0.02 mg/BSP was found to be effective in catalyzing an industrially challenging feedstock (68.77% w/w free fatty acids, 20.48% w/w triglycerides) to 98% fatty acid methyl esters (FAME). In a comparative investigation with polyurethane as matrix, higher trans/esterification facilitation was observed with AC. Benefiting from the oleophilicity of AC; denaturation effect from methanol on the lipases was reduced. Surface characterization with FE-SEM, XPS and FT-IR evidenced effective cell-matrix adhesion and a retention of the AC's intrinsic properties. The advantageous tribology of AC ensured recyclability of the matrix for fresh cells immobilization. Comparable FAME (98.08% w/w) was achieved with the recycled matrix in successive batches. The spent-matrix valorization approach, thus, proposes sustainable biorefineries with immobilized lipase catalyzed biodiesel production.

    AMER CHEMICAL SOC, 2019年03月04日, ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 7 (5), 5010 - 5017, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoshi Wakai, Nanami Nakashima, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

    Aspergillus oryzae, a filamentous fungus, can secrete large amounts of enzymes extracellularly. We constructed a genetically engineered A. oryzae that simultaneously produced cellobiohydrolase, endoglucanase, and β-glucosidase by integrating multiple copies of the genes encoding these cellulases into fungal chromosomes. The resulting strain possessed 5-16 copies of each cellulase gene within the chromosome and showed approximately 10-fold higher activity versus single integration strains. Copy number polymorphisms were attributed to differences in flanking region sequence for the integrated gene fragments. Furthermore, we found that the P-sodM/T-glaB set demonstrated the strongest transcription levels per gene copy number. We therefore modified promoter/terminator set and cellulase gene combinations based on this polymorphism and transcription level data, with the resulting transformant showing 40-fold higher cellulolytic activity versus the single integration strain. This designed expression method could be useful for the overexpression of multiple enzymes and pathway flux control-mediated metabolic engineering in A. oryzae.

    2019年03月, Bioresource technology, 276, 146 - 153, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Musashi Takenaka, Jae M Lee, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

    Actinobacteria plays a key role in the cycling of organic matter in soils. They secret biomass-degrading enzymes that allow it to produce the unique metabolites that originate in plant biomass. Although past studies have focused on these unique metabolites, a large-scale screening of Actinobacteria is yet to be reported to focus on their biomass-degrading ability. In the present study, a rapid and simple method is constructed for a large-scale screening, and the novel resources that form the plant biomass-degrading enzyme cocktail are identified from 850 isolates of Actinobacteria. As a result, Nonomuraea fastidiosa secretes a biomass degrading enzyme cocktail with the highest enzyme titer, although cellulase activities are lower than a commercially available enzyme. So the rich accessory enzymes are suggested to contribute to the high enzyme titer for a pretreated bagasse with a synergistic effect. Additionally, an optimized cultivation method of biomass induction caused to produce the improved enzyme cocktail indicated strong enzyme titers and a strong synergistic effect. Therefore, the novel enzyme cocktails are selected via the optimized method for large-scale screening, and then the enzyme cocktail can be improved via the optimized production with biomass-induction.

    2019年03月, Biotechnology journal, 14 (3), e1700744, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana

    AMOAH Jerome, HASUNUMA Tomohisa, OGINO Chiaki, KONDO Akihiko

    2019年02月, Biochemical Engineering Journal, 142, 117 - 123, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rahmani N, Kahar P, Lisdiyanti P, Lee J, Yopi, Prasetya B, Ogino C, Kondo A

    A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.

    2019年01月, Bioresource technology, 272, 315 - 325, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Hiroaki Akasaka, Naritoshi Mukumoto, Masao Nakayama, Tianyuan Wang, Ryuichi Yada, Yasuyuki Shimizu, Sachiko Inubushi, Katsusuke Kyotani, Keisuke Okumura, Masanori Miyamoto, Ai Nakaoka, Kenta Morita, Yuya Nishimura, Chiaki Ogino, Ryohei Sasaki

    © 2019, King Abdulaziz City for Science and Technology. Nanoparticles (NPs) are useful for radiotherapy. Currently, efforts are underway globally for the development of novel titanium dioxide NPs (TiO2-NPs) that exhibit both contrast effects and anti-tumor effects. In this study, the image contrast properties of TiO2-NPs were evaluated using a clinical magnetic resonance imaging (MRI) system and a clinical computed tomography (CT) scanner, as the use of TiO2-NPs as an anti-cancer agent has been reported in several reports. An obvious difference in visualization was observed between the control and TiO2-NP samples on T2-weighted images. These results suggest that TiO2 can potentially be used as a novel theranostic drug with radiosensitizing ability and radiological diagnostic ability, through modification of chemical groups on its surface, and as a component of drug delivery systems.

    2019年, Applied Nanoscience (Switzerland), 1 - 6, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yoshinori Miyata, Kunimasa Sagata, Yoshiko Yamazaki, Hiroshi Teramura, Yoshiaki Hirano, Chiaki Ogino, Yuichi Kita

    © 2018 American Chemical Society. Hydrothermal liquefaction (HTL) is a promising technique for converting biomass feedstocks to fuel and fine chemicals. A metallic Fe additive increases the water-soluble (WS) products of HTL, although the mechanism is unclear. Herein, commercially available carbohydrates (poly- and monosaccharides) and lignin isolated by enzymatic saccharification of palm empty fruit bunch were used as model substrates in the evaluation of the effect of Fe on HTL product composition. For carbohydrates, Fe and oxidized Fe synergistically contributed to the production of light compounds in the WS fraction by accelerating the retro-aldol condensation of sugars and suppressing the recondensation of unstable intermediates. The reactive intermediates could be stabilized by an electron-transfer-type reduction. On the other hand, Fe showed minimal effect on the HTL of enzymatic lignin, which was mainly converted to water-insoluble products. The results for the model substrates provided a picture of the overall pathway of Fe-assisted HTL of biomass.

    American Chemical Society, 2018年11月07日, Industrial and Engineering Chemistry Research, 57 (44), 14870 - 14877, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Yopi, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.

    2018年10月, Bioresource technology, 265, 542 - 547, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Yuji Noguchi, Norimasa Kashiwagi, Atsuko Uzura, Chiaki Ogino, Akihiko Kondo, Haruo Ikeda, Masahiro Sota

    BACKGROUND: Genetic tools including constitutive and inducible promoters have been developed over the last few decades for strain engineering in Streptomyces. Inducible promoters are useful for controlling gene expression, however only a limited number are applicable to Streptomyces. The aim of this study is to develop a controllable protein expression system based on an inducible promoter using sugar inducer, which has not yet been widely applied in Streptomyces. RESULTS: To determine a candidate promoter, inducible protein expression was first examined in Streptomyces avermitilis MA-4680 using various carbon sources. Xylose isomerase (xylA) promoter derived from xylose (xyl) operon was selected due to strong expression of xylose isomerase (XylA) in the presence of D-xylose. Next, a xylose-inducible protein expression system was constructed by investigating heterologous protein expression (chitobiase as a model protein) driven by the xylA promoter in Streptomyces lividans. Chitobiase activity was detected at high levels in S. lividans strain harboring an expression vector with xylA promoter (pXC), under both xylose-induced and non-induced conditions. Thus, S. avermitilis xylR gene, which encodes a putative repressor of xyl operon, was introduced into constructed vectors in order to control protein expression by D-xylose. Among strains constructed in the study, XCPR strain harboring pXCPR vector exhibited strict regulation of protein expression. Chitobiase activity in the XCPR strain was observed to be 24 times higher under xylose-induced conditions than that under non-induced conditions. CONCLUSION: In this study, a strictly regulated protein expression system was developed based on a xylose-induced system. As far as we could ascertain, this is the first report of engineered inducible protein expression in Streptomyces by means of a xylose-induced system. This system might be applicable for controllable expression of toxic products or in the field of synthetic biology using Streptomyces strains.

    2018年09月21日, Microbial cell factories, 17 (1), 151 - 151, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    A two-stage selection process was applied to eight oleaginous yeast strains from the Lipomyces genera. In the primary selection stage, a nitrogen-limited mineral medium (-NMM) that contained a mixture of glucose and xylose as a carbon source was used to evaluate the lipid-accumulating abilities of the yeast strains. The strains L. doorenjongii, L. orientalis, and L. starkeyi were selected as the potential strains in the primary selection. These three strains exhibited a remarkable ability to simultaneously assimilate glucose and xylose and achieved a cell biomass of more than 30 g/L. The values for lipid content in the selected strains were 57.89 ± 1.92, 56.38 ± 1.93, and 77.14 ± 1.55% for L. doorenjongii, L. orientalis, and L. starkeyi, respectively. In the secondary selection, when the -NMM medium contained an inhibitory chemical compound (ICC), the selected strains showed a different tolerance level against each of the typical inhibitor compounds. However, L. starkeyi accumulated the highest lipid content and yield at 68.24 ± 2.48% and 0.19 ± 0.00 (w/w), respectively. L. starkeyi accumulated high levels of intracellular lipid and tolerated the ICC. The composition of fatty acid methyl esters (FAMEs) was unaltered by the presence of ICC and the major FAMEs consisted of oleic, palmitic, stearic, palmitoleic and linoleic acids.

    Elsevier B.V., 2018年09月15日, Biochemical Engineering Journal, 137, 182 - 191, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Izzat F M Suffian, Julie T-W Wang, Farid N Faruqu, Julio Benitez, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo, Khuloud T Al-Jamal

    Hepatitis B virus core (HBc) particles acquire the capacity to disassemble and reassemble in a controlled manner, allowing entrapment and delivery of drugs and macromolecules to cells. HBc particles are made of 180-240 copies of 21 kDa protein monomers, assembled into 30-34 nm diameter icosahedral particles. In this study, we aimed at formulating HBc particles for the delivery of siRNA for gene silencing in vitro and in vivo. We have previously reported recombinant HBc particles expressing ZHER2 affibodies, specifically targeting human epidermal growth receptor 2 (HER2)-expressing cancer cells (ZHER2-ΔHBc). siRNA was encapsulated within the ZHER2-ΔHBc particles following disassembly and reassembly. The ZHER2-ΔHBc-siRNA hybrids were able to secure the encapsulated siRNA from serum and nucleases in vitro. Enhanced siRNA uptake in HER2-expressing cancer cells treated with ZHER2-ΔHBc-siRNA hybrids was observed compared to the nontargeted HBc-siRNA hybrids in a time- and dose-dependent manner. A successful in vitro polo-like kinase 1 (PLK1) gene knockdown was demonstrated in cancer cells treated with ZHER2-ΔHBc-siPLK1 hybrids, to levels comparable to commercial transfecting reagents. Interestingly, ZHER2-ΔHBc particles exhibit intrinsic capability of reducing the solid tumor mass, independent of siPLK1 therapy, in an intraperitoneal tumor model following intraperitoneal injection.

    2018年07月27日, ACS applied nano materials, 1 (7), 3269 - 3282, 英語, 国際誌

    研究論文(学術雑誌)

  • Yota Tsuge, Hideo Kawaguchi, Shogo Yamamoto, Yoshiko Nishigami, Masahiro Sota, Chiaki Ogino, Akihiko Kondo

    Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.

    2018年07月, Bioscience, biotechnology, and biochemistry, 82 (7), 1252 - 1259, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • 森田 健太, 鈴木 貴弘, 西村 勇哉, K. Matsumoto, 沼子 千弥, K. Sato, M. Nakayama, R. Sasaki, 荻野 千秋, 近藤 昭彦

    Polyacrylic acid (PAA)-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers. PAA-TiOx NPs were synthesized from commercial TiO2 nanoparticles that were modified with PAA and functionalized by H2O2 treatment. To realize practical clinical uses for PAA-TiOx NPs, their tissue distribution and acute toxicity were evaluated using healthy mice and mice bearing tumors derived from xenografted MIAPaCa-2 human pancreatic cancer cells. Healthy mice were injected with PAA-TiOx NPs at 25 mg/kg body weight via the tail vein, and tumor-bearing mice were injected either into the tumor locally or via the tail vein. The concentration of PAA-TiOx NPs in major organs was determined over time using inductively coupled-plasma atomic emission spectrometry. After 1 h, 12% of the PAA-TiOx NP dose had accumulated in the tumor, and 2.8% of the dose remained after 1 week. Such high accumulation could be associated with enhanced permeability and retention effects of the tumor, as PAA-TiOx NPs are composed of inorganic particles and polymers, without tumor-targeting molecules. The liver accumulated the largest proportion of the injected nanoparticles, up to 42% in tumor-bearing mice. Blood biochemical parameters were also investigated after intravenous injection of PAA-TiOx NPs in healthy mice. PAA-TiOx NPs invoked a slight change in various liver-related biochemical parameters, but no liver injury was observed over the practical dose range. In the future, PAA-TiOx NPs should be modified to prevent accumulation in the liver and minimize risk to patients.

    2018年07月, Journal of Bioscience and Bioengineering, 126 (1), 119 - 125, 英語, 国内誌

    [査読有り]

    研究論文(学術雑誌)

  • Ario B. Juanssilfero, Prihardi Kahar, Rezky L. Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    Oleaginous microbes can convert substrates such as carbon dioxide, sugars, and organic acids to single-cell oils (SCOs). Among the oleaginous microorganisms, Lipomyces starkeyi is a particularly well-suited host given its impressive native abilities, including the capability to utilize a wide variety of carbon sources. In this work, the potential of L. starkeyi NBRC10381 to produce SCOs in a synthetically nitrogen-limited mineral medium (-NMM) was investigated by differing the inoculum size using glucose and/or xylose as a carbon source. Fermentation using glucose and xylose as mixed carbon sources generated the highest production of biomass at 40.8 g/L, and achieved a lipid content of 84.9% (w/w). When either glucose or xylose was used separately, the totals for achieved lipid content were 79.6% (w/w) and 85.1% (w/w), respectively. However, biomass production was higher for glucose than for xylose (30.3 vs. 28.7 g/L, respectively). This study describes the first simultaneous achievement of higher levels of cell mass and lipid production using glucose and/or xylose as the carbon sources in different inoculum sizes.

    Elsevier B.V., 2018年06月01日, Journal of Bioscience and Bioengineering, 125 (6), 695 - 702, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Kumiko Yoshihara, Kiyotaka Y Hara, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    BACKGROUND: L-Arabinose is the second most abundant component of hemicellulose in lignocellulosic biomass, next to D-xylose. However, few microorganisms are capable of utilizing pentoses, and catabolic genes and operons enabling bacterial utilization of pentoses are typically subject to carbon catabolite repression by more-preferred carbon sources, such as D-glucose, leading to a preferential utilization of D-glucose over pentoses. In order to simultaneously utilize both D-glucose and L-arabinose at the same rate, a modified metabolic pathway was rationally designed based on metabolome analysis. RESULTS: Corynebacterium glutamicum ATCC 31831 utilized D-glucose and L-arabinose simultaneously at a low concentration (3.6 g/L each) but preferentially utilized D-glucose over L-arabinose at a high concentration (15 g/L each), although L-arabinose and D-glucose were consumed at comparable rates in the absence of the second carbon source. Metabolome analysis revealed that phosphofructokinase and pyruvate kinase were major bottlenecks for D-glucose and L-arabinose metabolism, respectively. Based on the results of metabolome analysis, a metabolic pathway was engineered by overexpressing pyruvate kinase in combination with deletion of araR, which encodes a repressor of L-arabinose uptake and catabolism. The recombinant strain utilized high concentrations of D-glucose and L-arabinose (15 g/L each) at the same consumption rate. During simultaneous utilization of both carbon sources at high concentrations, intracellular levels of phosphoenolpyruvate declined and acetyl-CoA levels increased significantly as compared with the wild-type strain that preferentially utilized D-glucose. These results suggest that overexpression of pyruvate kinase in the araR deletion strain increased the specific consumption rate of L-arabinose and that citrate synthase activity becomes a new bottleneck in the engineered pathway during the simultaneous utilization of D-glucose and L-arabinose. CONCLUSIONS: Metabolome analysis identified potential bottlenecks in D-glucose and L-arabinose metabolism and was then applied to the following rational metabolic engineering. Manipulation of only two genes enabled simultaneous utilization of D-glucose and L-arabinose at the same rate in metabolically engineered C. glutamicum. This is the first report of rational metabolic design and engineering for simultaneous hexose and pentose utilization without inactivating the phosphotransferase system.

    BioMed Central Ltd., 2018年05月17日, Microbial cell factories, 17 (1), 76 - 76, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Hideo Kawaguchi, Chiaki Ogino, Takashi Sazuka, Akihiko Kondo

    © 2017 We investigated the use of low concentrations of butanol (<40%, all v/v) as an organosolv pretreatment to fractionate lignocellulosic biomass into cellulose, hemicellulose, and lignin. The pretreatment conditions were optimized for sorghum bagasse by focusing on four parameters: butanol concentration, sulfuric acid concentration, pretreatment temperature, and pretreatment time. A butanol concentration of 25% or higher together with 0.5% or higher acid was effective for removing lignin while retaining most of the cellulose in the solid fraction. The highest cellulose (84.9%) and low lignin (15.3%) content were obtained after pretreatment at 200 °C for 60 min. Thus, pretreatment comprising 25% butanol, 0.5% acid, 200 °C, and 60 min process time was considered optimal. Enzymatic saccharification and fermentation by Saccharomyces cerevisiae produced 61.9 g/L ethanol from 200 g/L solid fraction obtained following pretreatment, and 10.2 g/L ethanol was obtained from the liquid fraction by xylose-utilizing S. cerevisiae following membrane nanofiltration to remove butanol.

    2018年03月, Bioresource Technology, 252, 157 - 164, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Amaliyah Rohsari Indah Utami, Yota Tsuge, Kosuke Kuroda, Chiaki Ogino, Tetsuya Taima, Joji Saito, Mitsugu Kimizu, Kenji Takahashi

    This is the first study to examine the minimum amount of choline acetate (ChOAc), a biocompatible cholinium ionic liquid (IL), for pretreatment of bagasse to achieve the sufficient enzymatic saccharification and co-fermentation from the obtained sugars. Pretreatment of bagasse was conducted using ChOAc at different ionic liquid biomass ratios (IL/biomass ratios of 0–3) to determine the minimum and sufficient IL/biomass ratio for enzymatic saccharification. To apply a small amount of viscous ChOAc to all of the bagasse powder, the ChOAc aqueous solution was first mixed with bagasse powder and the suspension was heated, which pretreated the biomass along with evaporation of water. The minimum IL/biomass ratio was found to be 1.5, which achieved cellulose and hemicellulose saccharification percentages of 95% and 93%, respectively, at low-loading (10 g/L) saccharification. The bagasse pretreated at the IL/biomass ratio of 1.5 was then applied to high-loading saccharification (100 g/L) and subsequent co-fermentation for ethanol production. Glucose and xylose concentrations were 56 g/L and 14 g/L, after 72 h of enzymatic saccharification at high-loading, which corresponded to cellulose and hemicellulose saccharification percentages of 95% and 65%, respectively. In the subsequent co-fermentation from a mixed sugar solution containing 27 g/L glucose and 7 g/L xylose, the ethanol concentration was 15 g/L at 24 h, which was 85% of the theoretical value for the sugar-based ethanol yield.

    Elsevier B.V., 2018年02月15日, Chemical Engineering Journal, 334, 657 - 663, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Megumi Abe, Takayuki Tsukegi, Kosuke Kuroda, Yota Tsuge, Chiaki Ogino, Kentaro Taki, Tetsuya Taima, Joji Saito, Mitsugu Kimizu, Kiyoshi Uzawa, Kenji Takahashi

    In the present study, we examined the efficacy of choline acetate (ChOAc, a cholinium ionic liquid))-assisted pretreatment of bagasse powder for subsequent mechanical nanofibrillation to produce lignocellulose nanofibers. Bagasse sample with ChOAc pretreatment and subsequent nanofibrillation (ChOAc/NF-bagasse) was prepared and compared to untreated control bagasse sample (control bagasse), bagasse sample with nanofibrillation only (NF-bagasse) and with ChOAc pretreatment only (ChOAc-bagasse). The specific surface area was 0.83 m2/g, 3.1 m(2)/g, 6.3 m(2)/g, and 32 m(2)/g for the control bagasse, ChOAc-bagasse, NF-bagasse, and the ChOAc/NF-bagasse, respectively. Esterified bagasse/polypropylene composites were prepared using the bagasse samples. ChOAc/NF-bagasse exhibited the best dispersion in the composites. The tensile toughness of the composites was 0.52 J/cm(3), 0.73 J/cm(3), 0.92 J/cm(3), and 1.29 J/cm(3) for the composites prepared using control bagasse, ChOAcbagasse, NF-bagasse, and ChOAc/NF-bagasse, respectively. Therefore, ChOAc pretreatment and subsequent nanofibrillation of bagasse powder resulted in enhanced tensile toughness of esterified bagasse/polypropylene composites.

    ELSEVIER SCI LTD, 2018年02月, CARBOHYDRATE POLYMERS, 182, 8 - 14, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Kobako Ochiai, Mao Eguchi, Kosuke Kuroda, Yota Tsuge, Chiaki Ogino, Tetsuya Taima, Kenji Takahashi

    This study compared the oxidative depolymerization potential between ionic liquid (IL)-pretreated/enzyme lignin, diluted acid (DA)-pretreated/enzyme lignin, and soda lignin of eucalyptus, by means of alkaline nitrobenzene or cupric oxide (CuO) oxidation which depolymerize lignin into phenolic aldehydes such as vanillin and syringaldehyde. Here, the IL-pretreated/enzyme lignin was prepared by 1-ethyl-3-methylimidazolium acetate pretreatment and subsequent enzymatic saccharification. Upon the oxidative depolymerization of lignin sample by alkaline nitrobenzene oxidation, total yield of vanillin and syringaldehyde was 36.6% for untreated control eucalyptus. In contrast, the yield was enhanced upto 48.0% for IL-pretreated/enzyme lignin. As for the soda lignin prepared by alkali cooking, the yield was 29.0%. As for the DA-pretreated/enzyme lignin prepared by DA pretreatment and subsequent enzymatic saccharification, the yield was 39.5%. Therefore, it was found that the IL-pretreated/enzyme lignin showed the highest yield of phenolic aldehydes (vanillin and syringaldehyde) via alkaline oxidation, namely, highest oxidative depolymerization potential among the lignin tested.

    Elsevier B.V., 2018年01月01日, Industrial Crops and Products, 111, 457 - 461, 英語

    研究論文(学術雑誌)

  • 川口 秀夫, 勝山 陽平, ト タンヤオ, 鶴田 祥子, 南 博道, 荻野 千秋, 大西 康夫, 近藤 昭彦

    Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by expressing the genes encoding a tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344 and 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (≤2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.

    一般社団法人 日本エネルギー学会, 2018年, バイオマス科学会議発表論文集, 13 (0), 135 - 136, 日本語

    [査読有り]

  • Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Euis Hermiati, Jaemin Lee, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

    The addition of enzymes that are capable of degrading hemicellulose has a potential to reduce the need for commercial enzymes during biomass hydrolysis in the production of fermentable sugars. In this study, a high xylanase producing actinomycete strain (Kitasatospora sp. ID06-480) and the first ethyl ferulate producing actinomycete strain (Nonomuraea sp. ID06-094) were selected from 797 rare actinomycetes, respectively, which were isolated in Indonesia. The addition (30%, v/v) of a crude enzyme supernatant from the selected strains in sugarcane bagasse hydrolysis with low-level loading (1 FPU/g-biomass) of Cellic® CTec2 enhanced both the released amount of glucose and reducing sugars. When the reaction with Ctec2 was combined with crude enzymes containing either xylanase or feruloyl esterase, high conversion yield of glucose from cellulose at 60.5% could be achieved after 72 h-saccharification.

    Japan Society for Bioscience Biotechnology and Agrochemistry, 2018年, Bioscience, Biotechnology and Biochemistry, 82 (5), 904 - 915, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Narges Ghobadi, Chiaki Ogino, Tomohiro Ogawa, Naoto Ohmura

    This study was focused on the intensification of alpha amylase activity by fungi. Submerged culture of Aspergillus oryzae as a non-Newtonian fluid with a complex rheology and morphology was used. The aim of this study was to enhance the hydrodynamics of the biological fluid for an improvement in cell-gas mass transfer. Therefore, we investigated the advantages of using a Swingstir® agitator that features an angular motion. Results showed that mixing time, mixing energy, and power-law model parameters using the Swingstir® all were lower than when a double Rushton turbine (DRT) was used at the same level of power consumption. The Swingstir® can produces a homogeneous and periodic-flow distribution. Moreover, KLa could be controlled throughout the fermentation process. Also, the morphology of pellets agitated by Swingstir® had a positive effect on mass transfer intensification, because the stress is applied by flexible shaft movement instead by the blade tip of impeller. Consequently, the alpha amylase activity and cell growth of a culture agitated by Swingstir® were higher than that developed using a DRT.

    Society of Chemical Engineers, Japan, 2018年, Journal of Chemical Engineering of Japan, 51 (2), 143 - 151, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryoko Ohno, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Shigeo Takumi

    © 2018, Genetics Society of Japan. All rights reserved. Wheat straw is one of the major attractive resources for low-cost raw materials for renewable energy, biofuels and biochemicals. However, like other sources of lignocellulosic biomass, straw is a heterogeneous material due to its mixed origin from different tissue and cell types. Here, to examine the genotypic effects on biorefinery usage of wheat straw, straw obtained from different wheat cultivars and experimental lines was pretreated with dilute acid. Significant differences between cultivars were observed in the concentrations of glucose and toxic byproducts of the liquid hydrolysates. A higher content of xylose than glucose was found in liquid hydrolysates from wheat straw, and the xylose content appeared to be affected by both environmental and genetic factors. Analysis using chromosome substitution lines of the common wheat cultivar Chinese Spring showed that chromosomes 2A and 3A from other wheat cultivars, Hope and Timstein, significantly increased the xylose content. However, no significant relationship was observed between the liquid hydrolysate xylose content and the glucose content obtained from enzymatic saccharification of the acid-insoluble residue. These results highlight the potential of wheat breeding to improve biomass-related traits in wheat straw.

    日本遺伝学会, 2018年, Genes and Genetic Systems, 93 (1), 1 - 7, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shimpei Aikawa, Kentaro Inokuma, Satoshi Wakai, Kengo Sasaki, Chiaki Ogino, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

    Background: The cyanobacterium Arthrospira platensis shows promise as a carbohydrate feedstock for biofuel production. The glycogen accumulated in A. platensis can be extracted by lysozyme-degrading the peptidoglycan layer of the bacterial cell walls. The extracted glycogen can be converted to ethanol through hydrolysis by amylolytic enzymes and fermentation by the yeast Saccharomyces cerevisiae. Thus, in the presence of lysozyme, a recombinant yeast expressing α-amylase and glucoamylase can convert A. platensis directly to ethanol, which would simplify the procedure for ethanol production. However, the ethanol titer and productivity in this process are lower than in ethanol production from cyanobacteria and green algae in previous reports. Results: To increase the ethanol titer, a high concentration of A. platensis biomass was employed as the carbon source for the ethanol production using a recombinant amylase-expressing yeast. The addition of lysozyme to the fermentation medium increased the ethanol titer, but not the ethanol productivity. The addition of CaCl2 increased both the ethanol titer and productivity by causing the delamination of polysaccharide layer on the cell surface of A. platensis. In the presence of lysozyme and CaCl2, ethanol titer, yield, and productivity improved to 48 g L-1, 93% of theoretical yield, and 1.0 g L-1 h-1 from A. platensis, corresponding to 90 g L-1 of glycogen. Conclusions: We developed an ethanol conversion process using a recombinant amylase-expressing yeast from A. platensis with a high titer, yield, and productivity by adding both lysozyme and CaCl2. The direct and highly productive conversion process from A. platensis via yeast fermentation could be applied to multiple industrial bulk chemicals.

    BioMed Central Ltd., 2018年, Biotechnology for biofuels, 11 (1), 50 - 50, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Tominaga, Hideo Kawaguchi, Yoshimi Hori, Tomohisa Hasunuma, Chiaki Ogino, Sachiyo Aburatani

    Measuring the concentrations of metabolites and estimating the reaction rates of each reaction step consisting of metabolic pathways are significant for an improvement in microorganisms used in maximizing the production of materials. Although the reaction pathway must be identified for such an improvement, doing so is not easy. Numerous reaction steps have been reported; however, the actual reaction steps activated vary or change according to the conditions. Furthermore, to build mathematical models for a dynamical analysis, the reaction mechanisms and parameter values must be known; however, to date, sufficient information has yet to be published for many cases. In addition, experimental observations are expensive. A new mathematical approach that is applicable to small sample data, and that requires no detailed reaction information, is strongly needed. S-system is one such model that can use smaller samples than other ordinary differential equation models. We propose a simplified S-system to apply minimal quantities of samples for a dynamic analysis of the metabolic pathways. We applied the model to the phenyl lactate production pathway of Escherichia coli. The model obtained suggests that actually activated reaction steps and feedback are inhibitions within the pathway.

    2018年, Bioinformatics and biology insights, 12, 1177932218775076 - 1177932218775076, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Ryoko Ohno, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Shigeo Takumi

    © 2018, Genetics Society of Japan. All rights reserved. Wheat straw is one of the major attractive resources for low-cost raw materials for renewable energy, biofuels and biochemicals. However, like other sources of lignocellulosic biomass, straw is a heterogeneous material due to its mixed origin from different tissue and cell types. Here, to examine the genotypic effects on biorefinery usage of wheat straw, straw obtained from different wheat cultivars and experimental lines was pretreated with dilute acid. Significant differences between cultivars were observed in the concentrations of glucose and toxic byproducts of the liquid hydrolysates. A higher content of xylose than glucose was found in liquid hydrolysates from wheat straw, and the xylose content appeared to be affected by both environmental and genetic factors. Analysis using chromosome substitution lines of the common wheat cultivar Chinese Spring showed that chromosomes 2A and 3A from other wheat cultivars, Hope and Timstein, significantly increased the xylose content. However, no significant relationship was observed between the liquid hydrolysate xylose content and the glucose content obtained from enzymatic saccharification of the acid-insoluble residue. These results highlight the potential of wheat breeding to improve biomass-related traits in wheat straw.

    2018年, Genes and Genetic Systems, 93 (1), 1 - 7, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naoko Okai, Takaya Masuda, Yasunobu Takeshima, Kosei Tanaka, Ken-Ichi Yoshida, Masanori Miyamoto, Chiaki Ogino, Akihiko Kondo

    Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.

    2017年12月, AMB Express, 7 (1), 130 - 130, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Norimasa Kashiwagi, Chiaki Ogino, Akihiko Kondo

    Bioproduction using microbes from biomass feedstocks is of interest in regards to environmental problems and cost reduction. Streptomyces as an industrial microorganism plays an important role in the production of useful secondary metabolites for various applications. This strain also secretes a wide range of extracellular enzymes which degrade various biopolymers in nature, and it consumes these degrading substrates as nutrients. Hence, Streptomyces can be employed as a cell factory for the conversion of biomass-derived substrates into various products. This review focuses on the following two points: (1) Streptomyces as a producer of enzymes for degrading biomass-derived polysaccharides and polymers; and, (2) wild-type and engineered strains of Streptomyces as a host for chemical production from biomass-derived substrates. (C) 2017 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1655 - 1663, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

    The worldwide market for plastics is rapidly growing, and plastics polymers are typically produced from petroleum-based chemicals. The overdependence on petroleum-based chemicals for polymer production raises economic and environmental sustainability concerns. Recent progress in metabolic engineering has expanded fermentation products from existing aliphatic acids or alcohols to include aromatic compounds. This diversity provides an opportunity to expand the development and industrial uses of high-performance bio-based polymers. However, most of the biomonomers are produced from edible sugars or starches that compete directly with food and feed uses. The present review focuses on recent progress in the microbial conversion of biomass into bio-based polymers, in which fermentative products from renewable feedstocks serve as biomonomers for the synthesis of bio-based polymers. In particular, the production of biomonomers from inedible lignocellulosic feedstocks by metabolically engineered microorganisms and the synthesis of bio-based engineered plastics from the biological resources are discussed. (C) 2017 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1664 - 1673, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoshi Wakai, Takayoshi Arazoe, Chiaki Ogino, Akihiko Kondo

    Filamentous fungi exhibit versatile abilities, including organic acid fermentation, protein production, and secondary metabolism, amongst others, and thus have applications in the medical and food industries. Previous genomic analyses of several filamentous fungi revealed their further potential as host microorganisms for bioproduction. Recent advancements in molecular genetics, marker recycling, and genome editing could be used to alter transformation and metabolism, based on optimized design carbolated with computer science. In this review, we detail the current applications of filamentous fungi and describe modern molecular genetic tools that could be used to expand the role of these microorganisms in bioproduction. The present review shed light on the possibility of filamentous fungi as host microorganisms in the field of bioproduction in the future. (C) 2017 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1314 - 1326, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jerome Amoah, Naoya Ishizue, Miki Ishizaki, Misa Yasuda, Kenji Takahashi, Kazuaki Ninomiya, Ryosuke Yamada, Akihiko Kondo, Chiaki Ogino

    This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (BGL, XYNII, EGII, CBHI and CBHII) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93 g/l ethanol whiles that for LUKP produced 0.71 g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass. (C) 2017 Published by Elsevier Ltd.

    ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1413 - 1420, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Prihardi Kahar, Eny Ida Riyanti, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Chiaki Ogino, Akihiko Kondo

    This study provides insight observation based on the gene expression and the metabolomic analysis of the natural robust yeast Saccharomyces cerevisiae NBRC849 during the fermentation in the medium containing inhibitory chemical complexes (ICC) at different concentrations. The tolerance mechanisms involved in the strain might have existed through the upregulation of genes involved in NAD(H)/NADP (H) cofactors generations (ALD6, ZWF1, GND1), membrane robustness for efflux pump (YOR1, PDR5, TPO3) and cation/polyamine transport (TPO3). The alteration of metabolic flux to the shikimic pathway was also found in this strain, resulted in the enhanced formation of aromatic amino acid required for cell survival. Enhanced expression of these genes as well as the increase of metabolic flux to shikimic pathway were suggested to result in the robustness of non-flocculating S. cerevisiae haploid strain. (C) 2017 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1436 - 1446, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Megumi Abe, Takayuki Tsukegi, Kosuke Kuroda, Masaaki Omichi, Kenji Takada, Mana Noguchi, Yota Tsuge, Chiaki Ogino, Kentaro Taki, Tetsuya Taima, Kiyoshi Uzawa, Kenji Takahashi

    For preparation of wood-plastic composites, pretreatment of lignocellulosic material is important to increase compatibility between matrix polymers and lignocellulosic fillers. In this study, choline acetate (ChOAc, a cholinium ionic liquid) assisted pretreatment of bagasse powder could improve properties of chemically modified bagasse/polypropylene composites. The composites had fewer voids derived from vascular bundles of the bagasse tissue when using ChOAc-pretreated bagasse powder compared with the case when using untreated bagasse powder. The tensile strength of the composites was 20 MPa, 35 MPa, and 40 MPa in the cases of polypropylene only, untreated bagasse powder, and ChOAc-pretreated bagasse powder, respectively. The elastic modulus was 0.7 GPa, 2.0 GPa, and 2.6 GPa in the case of polypropylene only, the case using untreated bagasse powder, and the case using ChOAc-pretreated bagasse powder, respectively. Thus, ChOAc pretreatment of bagasse powder could enhance the blending and the tensile property of propionylated bagasse/polypropylene composites compared with those of the composites made using untreated bagasse powder.

    ELSEVIER SCIENCE BV, 2017年12月, INDUSTRIAL CROPS AND PRODUCTS, 109, 158 - 162, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Alex Prima, Kiyotaka Y. Hara, Apridah Cameliawati Djohan, Norimasa Kashiwagi, Prihardi Kahar, Jun Ishii, Hideki Nakayama, Fumiyoshi Okazaki, Bambang Prasetya, Akihiko Kondo, Yopi, Chiaki Ogino

    This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/ mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, beta-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources. (C) 2017 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1400 - 1406, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    Lipid from Chlamydomonas sp. JSC4 was used as a feedstock for biodiesel production. The lipid was found to contain high amounts of phospholipids and free fatty acid in addition to the triglycerides. Two enzymatic methods for the efficient conversion of the heterogenous lipid to fatty acid methyl esters (FAME) were carried out. The method using either a lipase cocktail containing Candida cylindracea lipase and Thermomyces lanuginosus lipase combination (m I) or immobilized Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cells (m II) were both successful. However, the method using lipase cocktail showed 30.8% relative stability after the fourth batch, whereas the whole-cell biocatalyst showed 98.1%. Although the whole-cell biocatalyst tolerated a wide range of water content, an exploration of the effect of water-methanol interaction on the biocatalytic process showed that 24% water and 7: 1 methanol to oil ratio is more favorable for FAME production. A higher initial methanol consumption rate facilitated a more stable system with the whole-cell biocatalyst, producing over 97% FAME in 32 h. The efficient conversion of a highly heterogenous substrate in the presence of high amounts of water could be an effective technique for the enzymatic conversion of microalgal lipids.

    ELSEVIER SCIENCE BV, 2017年12月, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koji Osumi, Sachiko Matsuda, Naoki Fujimura, Kentaro Matsubara, Minoru Kitago, Osamu Itano, Chiaki Ogino, Nobuaki Shimizu, Hideaki Obara, Yuko Kitagawa

    Surgical site infections continue to be a common complication affecting surgical prognosis. Reactive oxygen species (ROS) are generated by ultrasound-irradiated titanium dioxide (TiO2) (UIT). Although excessive ROS production can cause cell damage, ROS at physiological levels mediate beneficial cellular responses, including angiogenesis. This study investigated whether UIT can promote healing of Escherichia coli-infected wounds. We used TiO2 and ultrasound irradiation using an ultrasonography machine at a frequency of 1.0 MHz and intensity of 0.4 W cm−2. These levels are not bactericidal in vitro therefore, we could study the effect of UIT on E. coli survival without interference of ultrasound effects. The number of cluster of differentiation 31-positive blood vessels, which are indicative of angiogenesis, was decreased by bacterial infection, and increased at the wound edges in the UIT-treated infected wounds, suggesting upregulation of neovascularization by UIT. Although UIT treatment did not decrease E. coli survival in vivo, it promoted healing of the infected wounds as evidenced by a significant decrease in the wound area in the UIT-treated mice. Our findings demonstrate that UIT promotes wound healing in surgical site infections and suggest beneficial use of the UIT-based approach as a novel therapeutic method to treat infected surgical wounds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2344–2351, 2017.

    John Wiley and Sons Inc., 2017年11月01日, Journal of Biomedical Materials Research - Part B Applied Biomaterials, 105 (8), 2344 - 2351, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

    A sensing system was constructed to monitor the target peptide via two aptamer-based sensors pinching. First, aptamers against somatostatin (SST) were selected via the systematic evolution of ligands by exponential enrichment, and four aptamers were selected from a single-stranded DNA library. Their specificities to SST were evaluated via surface plasmon resonance and atomic force microscopy (AFM). Next, two aptamers with higher specificities to SST were used as aptamer-based sensors; one aptamer was modified with a chip, and the other was modified with a probe. Based on AFM system, the probe was surveyed on the chip in SST solutions, simultaneously measuring an interactive force. The label-free SST could be detected, and then the change in its concentration could be monitored at levels that ranged from 2 to 2000 nM. The interactive force of a single pair was approximately 45 pN, and the molecule number was associated with the interactive force. Therefore, we could firstly select the aptamers against somatostatin, and the sandwich-like monitoring system can be used to promote peptide sensor or monitoring system using an aptamer-based sensor. (C) 2017 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2017年11月, SENSORS AND ACTUATORS B-CHEMICAL, 252, 813 - 821, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

    A split aptamers-based aptasensor is applied to peptide sensing using atomic force microscopy (AFM). Somatostatin (SST) is targeted as a model peptide. The aptamer against SST is split, and each part is chemically modified with a probe or a chip. Hybridized aptamers can detect label-free SSTs and monitor changes in their concentration from 2 nM to 2000 nM as an index of interactive force. The change in an interactive force could be firstly evaluated via Hill model. Additionally, the analysis based on the fitting enables clarification of the differences in hybridization and in interaction, as well as an estimation of the number of SSTs. The proposed sensing system can promote peptide sensing based on the use of an aptasensor. (C) 2017 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2017年11月, SENSORS AND ACTUATORS B-CHEMICAL, 252, 600 - 605, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama, Akihiko Kondo

    The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.

    2017年08月, Applied microbiology and biotechnology, 101 (15), 6007 - 6014, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Yohei Katsuyama, Du Danyao, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi, Ahikiko Kondo

    Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and a precursor for biologically active compounds and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by introducing the genes encoding a 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa and tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (ae<currency>2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. In SHF, the formation of by-product acetate and the accumulation of potential fermentation inhibitors increased significantly with kraft pulp hydrolysate than filter paper hydrolysate. The combination of these inhibitors had synergistic effects on caffeic acid fermentation at low concentrations. With lower loads of cellulase in SSF, less potential fermentation inhibitors (furfural, 5-hydroxymethyfurfural, and 4-hydroxylbenzoic acid) accumulated in the medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.

    SPRINGER, 2017年07月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101 (13), 5279 - 5290, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryosuke Yamada, Kazunori Nakashima, Nanami Asai-Nakashima, Wataru Tokuhara, Nobuhiro Ishida, Satoshi Katahira, Noriho Kamiya, Chiaki Ogino, Akihiko Kondo

    Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL.

    HUMANA PRESS INC, 2017年05月, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 182 (1), 229 - 237, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nanik Rahmani, 柏木 紀賢, J.M.LEE, S. Niimi-Nakamura, 松本 華, Prihardi Kahar, P.Lisdiyanti, Yopi, Bambang Prasetya, 荻野 千秋, 近藤 昭彦

    Mannan endo-1,4-beta-mannosidase(commonly known as beta-mannanase) catalyzes a random cleavage of the beta-D-1,4mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a beta-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected. We obtained a beta-mannanase from this strain, and an amino acid sequence of this Kitasatospora beta-mannanase showed a 58-71% similarity with the amino acid sequences of Streptomyces beta-mannanases. The Kitasatospora beta-mannanase showed a significant level of activity (944 U/mg) against locust bean gum (0.5% w/v) and a potential for oligosaccharide production from various mannan polymers. The beta-mannanase might be beneficial particularly in the enzymatic production of MOS for applications of mannan utilization.

    BIOMED CENTRAL LTD, 2017年05月, AMB Express, 7, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Izzat Fahimuddin Bin Mohamed Suffian, Mitla Garcia-Maya, Paul Brown, Tam Bui, Yuya Nishimura, Amir Rafiq Bin Mohammad Johari Palermo, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

    An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circular Dichroism (CD), respectively. The modified method produced similar to 3-fold higher yield and greater purity of wild type HBc particles than the conventional method. Our results demonstrated that the modified method produce a better yield and purity of HBc particles in an E. coli-expression system, which are fully characterised and suitable to be used for drug delivery applications.

    NATURE PUBLISHING GROUP, 2017年03月, SCIENTIFIC REPORTS, 7, 43160, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    The presence of high levels of free fatty acids (FFA) in oil is a barrier to one-step biodiesel production. Undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. This work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides (TAG). A partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic production of fatty acid methyl ester (FAME). Whole-cell Candida antarctica lipase B-expressing Aspergillus oryzae, and Novozym 435 produced only 75.2 and 73.5% FAME, respectively. Fusarium heterosporum lipase-expressing A. oryzae produced more than 93% FAME in 72 h using three molar equivalents of methanol. FFA and TAG were converted simultaneously in the presence of increasing water content that resulted from esterification. Therefore, F. heterosporum lipase with a noted high level of tolerance of water could be useful in the industrial production of biodiesel from feedstock that has high proportion of free fatty acids.

    WILEY-V C H VERLAG GMBH, 2017年03月, BIOTECHNOLOGY JOURNAL, 12 (3), 1600400, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Musashi Takenaka, Takuya Kobayashi, Kentaro Inokuma, Tomohisa Hasunuma, Tatsuo Maruyama, Chiaki Ogino, Akihiko Kondo

    The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the interactive force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface. (C) 2016 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2017年03月, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Izzat Fahimuddin Bin Mohamed Suffian, Julie Tzu-Wen Wang, Naomi O. Hodgins, Rebecca Klippstein, Mitla Garcia-Maya, Paul Brown, Yuya Nishimura, Hamed Heidari, Sara Bals, Jane K. Sosabowski, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

    Hepatitis B Virus core (HBc) particles have been studied for their potential as drug delivery vehicles for cancer therapy. HBc particles are hollow nano-particles of 30-34 nm diameter and 7 nm thick envelopes, consisting of 180-240 units of 21 kDa core monomers. They have the capacity to assemble/dis-assemble in a controlled manner allowing encapsulation of various drugs and other biomolecules. Moreover, other functional motifs, i.e. receptors, receptor binding sequences, peptides and proteins can be expressed. This study focuses on the development of genetically modified HBc particles to specifically recognise and target human epidermal growth factor receptor-2 (HER2)-expressing cancer cells, in vitro and in vivo, for future cancer therapy. The non-specific binding capacity of wild type HBc particles was reduced by genetic deletion of the sequence encoding arginine-rich domains. A specific HER2-targeting was achieved by expressing the ZHER2 affibodies on the HBc particles surface. In vitro studies showed specific uptake of ZHER2-AHBc particles in HER2 expressing cancer cells. In vivo studies confirmed positive uptake of ZHER2-ABBc particles in HER2-expressing tumours, compared to non-targeted AHBc particles in intraperitoneal tumour-bearing mice models. The present results highlight the potential of these nanocarriers in targeting HER2-positive metastatic abdominal cancer following intra-peritoneal administration. (C) 2016 The Authors. Published by Elsevier Ltd.

    ELSEVIER SCI LTD, 2017年03月, BIOMATERIALS, 120, 126 - 138, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Musashi Takenaka, Yuzo Okumura, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

    DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the interactive force in T10-A10 was measured by AFM, which returned an average interactive force of approximately 350 pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFMSELEX. (C) 2016 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2017年02月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27 (4), 954 - 957, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Narges Ghobadi, Chiaki Ogino, Kaoru Yamabe, Naoto Ohmura

    A Fullzone (FZ) impeller was used in the first study of the characteristics involved in the fermentation of Aspergillus oryzae. Both the experimental and simulation results of this study revealed novel findings into the positive relationship between the global-axial mixing patterns of a FZ impeller and fermentation efficiency. The mixing results when using the FZ impeller compared with a double Rushton turbine (DRT) impeller indicated that the culture mixed by the FZ resulted in a more homogeneous medium with higher values for oxygen mass transfer, cell growth rate, and alpha amylase activity. The simulation of fluid flow was done in a laminar regime using a two-fluid model. According to the simulation results, the maximum shear stress when using the DRT was higher than that with the FZ at the same power input (P-in). A high degree of local shear stress and the shear rate near the turbine blade of the DRT resulted in cell damage and a reduction in the enzyme activity, biomass, pellet diameter, and dissolved oxygen concentration. Calculations using the Brown equation showed that the maximum and average shear rates during mixing with the FZ impeller were lower than that when using the DRT. Therefore, the use of an FZ impeller, particularly at low Phi, enhanced the cultivation of A. oryzae. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2017年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123 (1), 101 - 108, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    The expression of epidermal growth factor receptor (EGFR) across a wide range of tumor cells has attracted attention for use as a tumor marker in drug delivery systems. Therefore, binding molecules with the ability to target EGFR have been developed. Among them, we focused on affibodies that are binding proteins derived from staphylococcal protein A. By displaying affibody (Z(EGFR)) binding to EGFR on the surface of a bio-nanocapsule (BNC) derived from a hepatitis B virus (HBV), we developed an altered BNC (Z(EGFR)-BNC) with a high specificity to EGFR-expressing cells. We considered two different types of Z(EGFR) (Z955 and Z1907), and found that the Z1907 dimer-displaying BNC ([Z1907[(2)-BNC) could effectively bind to EGFR-expressing cells and deliver drugs to the cytosol. Since this study showed that [Z1907](2)-BNC could target EGFR-expressing cells, we would use this particle as a drug delivery carrier for various cancer cells expressing EGFR. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license.

    PERGAMON-ELSEVIER SCIENCE LTD, 2017年01月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27 (2), 336 - 341, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Narges Ghobadi, Naoya Sasakura, Satoshi Wakai, Chiaki Ogino, Kaoru Yamabe, Naoto Ohmura

    2017年, Journal of Bioprocessing & Biotechniques, 7 (2), 300: 1 - 5, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryutaro Morita, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Hiroshi Fukayama

    Production of bioethanol from rice straw has attracted attention from the point of effective use of agricultural residue. Starch content is an important determinant for bioethanol production from rice straw. The overexpression of CO2 -responsive CCT protein (CRCT), which is the positive regulator of starch synthesis in vegetative organs, notably increased the starch content in rice straw. To produce the bioethanol from rice straw, the dilute acid pretreatment is a general pretreatment method. Importantly, the glucose yields in liquid hydrolyzate after dilute acid pretreatment was markedly increased in the CRCT overexpression lines compared with non-transgenic rice. In addition, the overexpression of CRCT enhanced the biomass production. In contrast, CRCT did not affect on the glucose yields from cellulose in acid-insoluble residue obtained after dilute acid pretreatment. With respect to byproduct in liquid hydrolyzate which inhibits the fermentation, the formic acid content was increased, whereas the furfural, 5-hydroxymethylfurfural and acetic acid contents were unchanged by the overexpression of CRCT. These results demonstrate that genetic engineering of CRCT is an effective method to increase the bioethanol production from rice straw.

    TAYLOR & FRANCIS LTD, 2017年, PLANT PRODUCTION SCIENCE, 20 (4), 441 - 447, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Teramura, Kengo Sasaki, Hideo Kawaguchi, Fumio Matsuda, Jun Kikuchi, Tomokazu Shirai, Takashi Sazuka, Masanori Yamasaki, Shigeo Takumi, Chiaki Ogino, Akihiko Kondo

    Bio-refinery processes require use of the most suitable lignocellulosic biomass for enzymatic saccharification and microbial fermentation. Glucose yield from biomass solid fractions obtained after dilute sulfuric acid (1%) pretreatment (at 180 degrees C) was investigated using 14, 8, and 16 varieties of rice, wheat, and sorghum, respectively. Biomass solid fractions of each crop showed similar cellulose content. However, glucose yield after enzymatic hydrolysis (cellulase loading at 6.6 filter paper unit/g-biomass) was different among the varieties of each crop, indicating genotypic differences for rice, wheat, and sorghum. Nuclear magnetic resonance method revealed that the high residual level of lignin aromatic regions decreased glucose yield from solid fraction of sorghum.

    TAYLOR & FRANCIS LTD, 2017年, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81 (8), 1650 - 1656, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryutaro Morita, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Hiroshi Fukayama

    Production of bioethanol from rice straw has attracted attention from the point of effective use of agricultural residue. Starch content is an important determinant for bioethanol production from rice straw. The overexpression of CO2 -responsive CCT protein (CRCT), which is the positive regulator of starch synthesis in vegetative organs, notably increased the starch content in rice straw. To produce the bioethanol from rice straw, the dilute acid pretreatment is a general pretreatment method. Importantly, the glucose yields in liquid hydrolyzate after dilute acid pretreatment was markedly increased in the CRCT overexpression lines compared with non-transgenic rice. In addition, the overexpression of CRCT enhanced the biomass production. In contrast, CRCT did not affect on the glucose yields from cellulose in acid-insoluble residue obtained after dilute acid pretreatment. With respect to byproduct in liquid hydrolyzate which inhibits the fermentation, the formic acid content was increased, whereas the furfural, 5-hydroxymethylfurfural and acetic acid contents were unchanged by the overexpression of CRCT. These results demonstrate that genetic engineering of CRCT is an effective method to increase the bioethanol production from rice straw.

    TAYLOR & FRANCIS LTD, 2017年, PLANT PRODUCTION SCIENCE, 20 (4), 441 - 447, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Quentin Schmetz, Guillaume Maniet, Nicolas Jacquet, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Aurore Richel

    It is commonly accepted that the current society needs to partially substitute fossil resources by renewable ones. Among many solutions, one approach consists in the development of biorefinery involving lignocellulosic biomass to produce bio-based materials and fuels. This study focuses on the comprehension of an organosolv treatment designed to break the complex lignocellulosic structure for high purity lignin extraction from tall fescue (Festuca arundinacea Schreb.). This grass benefits from an increasing interest in Western Europe and has been suggested as feedstock for biorefinery. However, its use as material for high purity lignin production has not been determined yet. Ethanol/water, 92/8% [v/v] with H2SO4 0.32 M was investigated at pilot scale under conventional heating (5 degrees C min(-1) during 30 min and stabilized at 148 degrees C for 5 min). Precipitated lignin were analyzed as well as the composition of side stream products (recovered cellulosic pulp and the aqueous hydrolysate). Lignin has been recovered at a purity level of 90% with a yield of 60%. The main contaminants were nitrogen containing compounds and degraded hemicelluloses. 2D-HSQC NMR (Two Dimension-Heteronuclear Single Quantum Correlation Nuclear Magnetic Resonance) revealed a co-extraction of ferulates and coumarates function as well as arabinoxylan. Cellulose was recovered at 53% purity with 60% yield. The conditions appear to be too harsh for tall fescue and led to significant amount of cellulose degradation. A process using a lower alcohol concentration will be developed to provide better yields of both cellulose and lignin. (C) 2016 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2016年12月, INDUSTRIAL CROPS AND PRODUCTS, 94, 308 - 317, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kenta Morita, Serika Miyazaki, Chiya Numako, Shinya Ikeno, Ryohei Sasaki, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo

    An induction of polyacrylic acid-modified titanium dioxide with hydrogen peroxide nanoparticles (PAA-TiO2/H2O2 NPs) to a tumor exerted a therapeutic enhancement of X-ray irradiation in our previous study. To understand the mechanism of the radiosensitizing effect of PAA-TiO2/H2O2 NPs, analytical observations that included DLS, FE-SEM, FT-IR, XAFS, and Raman spectrometry were performed. In addition, highly reactive oxygen species (hROS) which PAA-TiO2/H2O2 NPs produced with X-ray irradiation were quantified by using a chemiluminescence method and a EPR spin-trapping method. We found that PAA-TiO2/H2O2 NPs have almost the same characteristics as PAA-TiO2. Surprisingly, there were no significant differences in hROS generation. However, the existence of H2O2 was confirmed in PAA-TiO2/H2O2 NPs, because spontaneous hROS production was observed w/o X-ray irradiation. In addition, PAA-TiO2/H2O2 NPs had a curious characteristic whereby they absorbed H2O2 molecules and released them gradually into a liquid phase. Based on these results, the H2O2 was continuously released from PAA-TiO2/H2O2 NPs, and then released H2O2 assumed to be functioned indirectly as a radiosensitizing factor.

    TAYLOR & FRANCIS LTD, 2016年12月, FREE RADICAL RESEARCH, 50 (12), 1319 - 1328, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    The feedstocks used for the production of bio-based chemicals have recently expanded from edible sugars to inedible and more recalcitrant forms of lignocellulosic biomass. To produce biobased chemicals from renewable polysaccharides, several bioprocessing approaches have been developed and include separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP). In the last decade, SHF, SSF, and CBP have been used to generate macromolecules and aliphatic and aromatic compounds that are capable of serving as sustainable, drop-in substitutes for petroleum-based chemicals. The present review focuses on recent progress in the bioprocessing of microbially produced chemicals from renewable feedstocks, including starch and lignocellulosic biomass. In particular, the technological feasibility of bio-based chemical production is discussed in terms of the feedstocks and different bioprocessing approaches, including the consolidation of enzyme production, enzymatic hydrolysis of biomass, and fermentation.

    CURRENT BIOLOGY LTD, 2016年12月, CURRENT OPINION IN BIOTECHNOLOGY, 42, 30 - 39, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Narges Ghobadi, Chiaki Ogino, Tomohiro Ogawa, Naoto Ohmura

    The rheology behavior of biological fluids particularly when the viscosity is high and rheology is complex, is an important issue to understand, particularly for studies in mass-transfer and for solving technical problems with mixing in stirred bioreactors. In this paper, the use of a Swingstir(A (R)) impeller during the fermentation of Aspergillus oryzae resulted in decreases from the parameters of a power-law model, in viscosity and in the thixotropic behavior of a cultivation broth. The results showed that both the K (L) a and the alpha amylase activity were improved when using the Swingstir(A (R)) in comparison with Fullzone(A (R)) impeller (FZ) at the same level of energy consumption. Increasing the pellet porosity during mixing via the Swingstir(A (R)) resulted in increases in oxygen mass transfer and the average shear stress.

    SPRINGER, 2016年11月, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 39 (11), 1793 - 1801, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Narges Ghobadi, Chiaki Ogino, Naoto Ohmura

    Background: The intensification of fermentation in stirred bioreactors is an attractive approach for commercial interests and industries that work with biochemical products. Alpha amylase is an enzymatic bio-products that is produced largely from Aspergillus oryzae. Using adaptable agitator can be an efficient way for stirred fermentation. Objective: This study, concentrated on enhancing the mixing process in order to intensify Aspergillus oryzae fermentation. A straight type of Maxblend® impeller was used as an agitator to investigate the incubation parameters compared with the use of a 6-blade double Rushton turbine. Method: Stirred fermentation was done in batch condition. Effect of flow pattern of mixing on fermentation parameters was investigated after each sampling. Results: The results showed that the Maxblend® significantly intensified both enzyme activity and growth rate at low and moderate rates of power consumption (P). The main reason for the decreases in the growth rate and the enzyme activity during agitation by the Rushton turbine at low and high Pv was the lack of oxygen and mycelial damage, respectively. Additionally, use of the Maxblend® significantly intensified the KLa at low and moderate rates of power consumption. Conclusion: Axial and uniform mixing by Maxblend® impeller was resulted in improving the fermentation characteristics and enzyme activity.

    Bentham Science Publishers B.V., 2016年10月01日, Open Chemical Engineering Journal, 10, 88 - 109, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Effect of Swingstir impeller on rheology and morphology of submerged fermentation

    Narges Ghobadi, Chiaki Ogino, Tomohiro Ogawa, Naoto Ohmura

    2016年09月, Proceedings of 5th Asian Conference on Mixing, 2016 (ACOM2016), 45 - 50, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

    Background: Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays beta-mannanase and beta-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source. Results: Parental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered beta-mannanase and beta-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-beta-D-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-beta-D-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate. Conclusions: We successfully displayed beta-mannanase and beta-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying beta-mannanase and beta-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering beta-mannanase and beta-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components.

    BIOMED CENTRAL LTD, 2016年09月, Biotechnology for Biofuels, 9 (1), 188, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keisuke Yamamoto, Kiyotaka Y. Hara, Toshihiko Morita, Akira Nishimura, Daisuke Sasaki, Jun Ishii, Chiaki Ogino, Noriyuki Kizaki, Akihiko Kondo

    Background: Red yeast, Xanthophyllomyces dendrorhous is the only yeast known to produce astaxanthin, an antioxidant isoprenoid (carotenoid) widely used in the aquaculture, food, pharmaceutical and cosmetic industries. The potential of this microorganism as a platform cell factory for isoprenoid production has been recognized because of high flux through its native terpene pathway. Recently, we developed a multiple gene expression system in X. dendrorhous and enhanced the mevalonate synthetic pathway to increase astaxanthin production. In contrast, the mevalonate synthetic pathway is suppressed by ergosterol through feedback inhibition. Therefore, releasing the mevalonate synthetic pathway from this inhibition through the deletion of genes involved in ergosterol synthesis is a promising strategy to improve isoprenoid production. An efficient method for deleting diploid genes in X. dendrorhous, however, has not yet been developed. Results: Xanthophyllomyces dendrorhous was cultivated under gradually increasing concentrations of antibiotics following the introduction of antibiotic resistant genes to be replaced with target genes. Using this method, double CYP61 genes encoding C-22 sterol desaturases relating to ergosterol biosynthesis were deleted sequentially. This double CYP61 deleted strain showed decreased ergosterol biosynthesis compared with the parental strain and single CYP61 disrupted strain. Additionally, this double deletion of CYP61 genes showed increased astaxanthin production compared with the parental strain and the single CYP61 knockout strain. Finally, astaxanthin production was enhanced by 1.4-fold compared with the parental strain, although astaxanthin production was not affected in the single CYP61 knockout strain. Conclusions: In this study, we developed a system to completely delete target diploid genes in X. dendrorhous. Using this method, we deleted diploid CYP61 genes involved in the synthesis of ergosterol that inhibits the pathway for mevalonate, which is a common substrate for isoprenoid biosynthesis. The resulting decrease in ergosterol biosynthesis increased astaxanthin production. The efficient method for deleting diploid genes developed in this study has the potential to improve industrial production of various isoprenoids in X. dendrorhous.

    BIOMED CENTRAL LTD, 2016年09月, MICROBIAL CELL FACTORIES, 15 (1), 155, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    The presence of phospholipid has been a challenge in liquid enzymatic biodiesel production. Among six lipases that were screened, lipase AY had the highest hydrolysis activity and a competitive transesterification activity. However, it yielded only 21.1% FAME from oil containing phospholipids. By replacing portions of these lipases with a more robust bioFAME lipase, CalT, the combination of lipase AY-CalT gave the highest FAME yield with the least amounts of free fatty acids and partial glycerides. A higher methanol addition rate reduced FAME yields for lipase DF-CalT and A10D-CalT combinations while that of lipase AY-CalT combination improved. Optimizing the methanol addition rate for lipase AY-CalT resulted in a FAME yield of 88.1% at 2 h and more than 95% at 6 h. This effective use of lipases could be applied for the rapid and economic conversion of unrefined oils to biodiesel. (C) 2016 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2016年07月, BIORESOURCE TECHNOLOGY, 211, 224 - 230, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Tsutomu Tanaka, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Zhenquan Tan, Yuya Nishimura, Hiroaki Akasaka, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo

    Background: Biological applications of nanoparticles are rapidly increasing, which introduces new possibilities to improve the efficacy of radiotherapy. Here, we synthesized titanium peroxide nanoparticles (TiOxNPs) and investigated their efficacy as novel agents that can potently enhance the effects of radiation in the treatment of pancreatic cancer. Methods: TiOxNPs and polyacrylic acid-modified TiOxNPs (PAA-TiOxNPs) were synthesized from anatase-type titanium dioxide nanoparticles (TiO(2)NPs). The size and morphology of the PAA-TiOxNPs was evaluated using transmission electron microscopy and dynamic light scattering. The crystalline structures of the TiO(2)NPs and PAA-TiOxNPs with and without X-ray irradiation were analyzed using X-ray absorption. The ability of TiOxNPs and PAA-TiOxNPs to produce reactive oxygen species in response to X-ray irradiation was evaluated in a cell-free system and confirmed by flow cytometric analysis in vitro. DNA damage after X-ray exposure with or without PAA-TiOxNPs was assessed by immunohistochemical analysis of gamma-H2AX foci formation in vitro and in vivo. Cytotoxicity was evaluated by a colony forming assay in vitro. Xenografts were prepared using human pancreatic cancer MIAPaCa-2 cells and used to evaluate the inhibition of tumor growth caused by X-ray exposure, PAA-TiOxNPs, and the combination of the two. Results: The core structures of the PAA-TiOxNPs were found to be of the anatase type. The TiOxNPs and PAA-TiOxNPs showed a distinct ability to produce hydroxyl radicals in response to X-ray irradiation in a dose-and concentration-dependent manner, whereas the TiO(2)NPs did not. At the highest concentration of TiOxNPs, the amount of hydroxyl radicals increased by >8.5-fold following treatment with 30 Gy of radiation. The absorption of PAA-TiOxNPs enhanced DNA damage and resulted in higher cytotoxicity in response to X-ray irradiation in vitro. The combination of the PAA-TiOxNPs and X-ray irradiation induced significantly stronger tumor growth inhibition compared to treatment with either PAA-TiOxNPs or X-ray alone (p < 0.05). No apparent toxicity or weight loss was observed for 43 days after irradiation. Conclusions: TiOxNPs are potential agents for enhancing the effects of radiation on pancreatic cancer and act via hydroxyl radical production; owing to this ability, they can be used for pancreatic cancer therapy in the future.

    BIOMED CENTRAL LTD, 2016年07月, RADIATION ONCOLOGY, 11 (1), 91, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Hirotaka Maruyama, Chiaki Ogino, Kenji Takahashi, Nobuaki Shimizu

    Abstract The present study demonstrates ultrasound-induced cell injury using a nickel-titanium dioxide (Ni-TiO2) alloy plate as a sonocatalyst and a cell culture surface. Ultrasound irradiation of cell-free Ni-TiO2 alloy plates with 1 MHz ultrasound at 0.5 W/cm2 for 30 s led to an increased generation of hydroxyl (OH) radicals compared to nickel-titanium (Ni-Ti) control alloy plates with and without ultrasound irradiation. When human breast cancer cells (MCF-7 cells) cultured on the Ni-TiO2 alloy plates were irradiated with 1 MHz ultrasound at 0.5 W/cm2 for 30 s and then incubated for 48 h, cell density on the alloy plate was reduced to approximately 50% of the controls on the Ni-Ti alloy plates with and without ultrasound irradiation. These results indicate the injury of MCF-7 cells following sonocatalytic OH radical generation by Ni-TiO2. Further experiments demonstrated cell shrinkage and chromatin condensation after ultrasound irradiation of MCF-7 cells attached on the Ni-TiO2 alloy plates, indicating induction of apoptosis.

    Elsevier, 2016年06月30日, Ultrasonics Sonochemistry, 28, 1 - 6, 英語

    研究論文(学術雑誌)

  • Takashi Goda, Hiroshi Teramura, Miki Suehiro, Kengo Kanamaru, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Masanori Yamasaki

    Rice straw is a promising resource for bioethanol production. Because the glucose content of pretreatment liquid hydrolysates is highly correlated with ethanol yield, the selection of appropriate rice cultivars is essential. The glucose content in liquid hydrolysates of pretreated rice straws of 208 diverse cultivars was evaluated in natural field in 2013 and 2014 using a novel high-throughput system. The glucose content of the rice straw samples varied across cultivars and was affected by environmental factors such as temperature and solar radiation. Several high-quality cultivars exhibiting high glucose content in both years were identified. The results of this study can aid in development of novel rice cultivars suitable as both feedstocks for bioethanol production and cooking.

    TAYLOR & FRANCIS LTD, 2016年05月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80 (5), 863 - 869, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Liu Z, Ho S.H, Sasaki Kengo, Haan R, Inokuma Kentaro, Ogino Chiaki, van Zyl W.H, Hasunuma Tomohisa, Kondo Akihiko

    Cellulosic biofuel is the subject of increasing attention. The main obstacle toward its economic feasibility is the recalcitrance of lignocellulose requiring large amount of enzyme to break. Several engineered yeast strains have been developed with cellulolytic activities to reduce the need for enzyme addition, but exhibiting limited effect. Here, we report the successful engineering of a cellulose-adherent Saccharomyces cerevisiae displaying four different synergistic cellulases on the cell surface. The cellulase-displaying yeast strain exhibited clear cell-to-cellulose adhesion and a "tearing" cellulose degradation pattern; the adhesion ability correlated with enhanced surface area and roughness of the target cellulose fibers, resulting in higher hydrolysis efficiency. The engineered yeast directly produced ethanol from rice straw despite a more than 40% decrease in the required enzyme dosage for high-density fermentation. Thus, improved cell-to-cellulose interactions provided a novel strategy for increasing cellulose hydrolysis, suggesting a mechanism for promoting the feasibility of cellulosic biofuel production.

    NATURE PUBLISHING GROUP, 2016年04月, Scientific Reports, 6, 24550, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Fumio Matsuda, Mami Okamoto, Tomokazu Shirai, Hideo Kawaguchi, Chiaki Ogino, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Jun Kikuchi, Akihiko Kondo

    Background: The primary components of lignocellulosic biomass such as sorghum bagasse are cellulose, hemicellulose, and lignin. Each component can be utilized as a sustainable resource for producing biofuels and bio-based products. However, due to their complicated structures, fractionation of lignocellulosic biomass components is required. Organosolv pretreatment is an attractive method for this purpose. However, as organosolv pretreatment uses high concentrations of organic solvents (>50 %), decreasing the concentration necessary for fractionation would help reduce processing costs. In this study, we sought to identify organic solvents capable of efficiently fractionating sorghum bagasse components at low concentrations. Results: Five alcohols (ethanol, 1-propanol, 2-propanol, 1-butanol, and 1-pentanol) were used for organosolv pretreatment of sorghum bagasse at a concentration of 12.5 %. Sulfuric acid (1 %) was used as a catalyst. With 1-butanol and 1-pentanol, three fractions (black liquor, liquid fraction containing xylose, and cellulose-enriched solid fraction) were obtained after pretreatment. Two-dimensional nuclear magnetic resonance analysis revealed that the lignin aromatic components of raw sorghum bagasse were concentrated in the black liquor fraction, although the major lignin side-chain (beta-O-4 linkage) was lost. Pretreatment with 1-butanol or 1-pentanol effectively removed p-coumarate, some guaiacyl, and syringyl. Compared with using no solvent, pretreatment with 1-butanol or 1-pentanol resulted in two-fold greater ethanol production from the solid fraction by Saccharomyces cerevisiae. Conclusions: Our results revealed that a low concentration (12.5 %) of a highly hydrophobic solvent such as 1-butanol or 1-pentanol can be used to separate the black liquor from the solid and liquid fractions. The efficient delignification and visible separation of the lignin-rich fraction possible with this method simplify the fractionation of sorghum bagasse.

    BIOMED CENTRAL LTD, 2016年02月, BIOTECHNOLOGY FOR BIOFUELS, 9, 27, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kohji Nobuta, Hiroshi Teramura, Hiroaki Ito, Chizuru Hongo, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Takashi Nishino

    Four types of kenaf bast fibers were prepared via a combination of Wise treatments, for delignification, and alkaline treatments, for the removal of hemicellulose. Each type of kenaf bast fiber with different refining processes were nano fibrillated by grinding. Resulting, cellulose nanofiber (CNF) sheet was obtained from CNF by vacuum filtration (Scheme 1). The structures and properties of these CNF sheets then were investigated to determine how the CNF components had affected these properties. All of the CNFs from different refining processes were classified as a cellulose I-beta type by X-ray diffraction. However, the mechanical properties (Young's modulus, tensile strength and toughness) of the CNF sheet with Wise treatment were higher than the properties of the other three CNF sheets. These results strongly suggested that alkaline treatment was unnecessary for the removal of hemicellulose, and that the application of the Wise treatment effectively imparted high mechanical properties to the cellulose microfiber.

    SPRINGER, 2016年02月, CELLULOSE, 23 (1), 403 - 414, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naoko Okai, Takanori Miyoshi, Yasunobu Takeshima, Hiroaki Kuwahara, Chiaki Ogino, Akihiko Kondo

    Protocatechuic acid (3,4-dihydroxybenzoic acid; PCA) serves as a building block for polymers and pharmaceuticals. In this study, the biosynthetic pathway for PCA from glucose was engineered in Corynebacterium glutamicum. The pathway to PCA-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (CPL) and 4-hydroxybenzoate hydroxylase (4-HBA hydroxylase). As C. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-HBA hydroxylase, we focused on expressing Escherichia coli CPL in a phenylalanine-producing strain of C. glutamicum ATCC21420. To secrete PCA, the gene (ubiC) encoding CPL from E. coli was expressed in C. glutamicum ATCC 21420 (strain F(UbiC)). The formation of 28.8 mg/L of extracellular 4-HBA (36 h) and 213 ± 29 mg/L of extracellular PCA (80 h) was obtained by the C. glutamicum strain F(UbiC) from glucose. The strain ATCC21420 was also found to produce extracellular PCA. PCA fermentation was performed using C. glutamicum strain F(UbiC) in a bioreactor at the optimized pH of 7.5. C. glutamicum F(UbiC) produced 615 ± 2.1 mg/L of PCA from 50 g/L of glucose after 72 h. Further, fed-batch fermentation of PCA by C. glutamicum F(UbiC) was performed with feedings of glucose every 24 h. The maximum production of PCA (1140.0 ± 11.6 mg/L) was achieved when 117.0 g/L of glucose was added over 96 h of fed-batch fermentation.

    2016年01月, Applied microbiology and biotechnology, 100 (1), 135 - 45, 英語, 国際誌

    [査読有り]

  • 川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

    Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

    一般社団法人 日本エネルギー学会, 2016年01月, バイオマス科学会議発表論文集, 11, 35 - 36, 日本語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Kengo Sasaki, Kiyotaka Y. Hara, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    This study aimed to optimize extracellular glutathione production by a Saccharomyces cerevisiae engineered strain and to concentrate the extracellular glutathione by membrane separation processes, including ultrafiltration (UF) and nanofiltration (NF). Synthetic defined (SD) medium containing 20 g L-1 glucose was fermented for 48 h; the fermentation liquid was passed through an UF membrane to remove macromolecules. Glutathione in this permeate was concentrated for 48 h to 545.1 +/- 33.6 mg L-1 using the NF membrane; this was a significantly higher concentration than that obtained with yeast extract peptone dextrose (YPD) medium following 96 h NF concentration (217.9 +/- 57.4 mg L-1). This higher glutathione concentration results from lower cellular growth in SD medium (final OD600 = 6.9 +/- 0.1) than in YPD medium (final OD600 = 11.0 +/- 0.6) and thus higher production of extracellular glutathione (16.0 +/- 1.3 compared to 9.2 +/- 2.1 mg L-1 in YPD medium, respectively). Similar fermentation and membrane processing of sweet sorghum juice containing 20 g L-1 total sugars provided 240.3 +/- 60.6 mg L-1 glutathione. Increased extracellular production of glutathione by this engineered strain in SD medium and subsequent UF permeation and NF concentration in shortend time may help realize industrial recovery of extracellular glutathione. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2016年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121 (1), 96 - 100, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    The presence of phospholipids in oil has been a major hurdle in the production of biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts. A density of phospholipids within the range of 10-30% could reduce both the rate of production and the final yield of biodiesel. Phospholipids in the oil leads to the formation of water-in-oil phospholipid-based reverse micelles. The water that activates the enzymatic process is observed to be trapped inside these reverse micelles. This has resulted in the inactivation of the reaction systems and has subsequently led to the deactivation of the immobilized lipase by the extended residence time of the added methanol. A reaction system involving gentle agitation and higher amount of water was found to reduce the reverse micelles formation. This simple technique improved the conversion efficiency by approximately 3-folds, producing a final biodiesel of more than 90%, using immobilized A. oryzae whole cells expressing Fusarium heterosporum lipase. This demonstrates that, the above technique could be successfully applied to the enzymatic biodiesel conversion of oils containing high amounts of phospholipids such as that from microalgae. (C) 2015 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2016年01月, BIOCHEMICAL ENGINEERING JOURNAL, 105, 10 - 15, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0 g l(-1)) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum. (C) 2015 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2015年12月, BIORESOURCE TECHNOLOGY, 198, 410 - 417, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Chiaki Ogino, Miki Ishizaki, Misa Yasuda, Nobuaki Shimizu, Kenji Takahashi

    Choline acetate (ChOAc), a cholinium ionic liquid (IL), was used for biomass pretreatment thereafter, saccharification of the pretreated biomass and glucose/xylose co-fermentation were conducted with different number of post-pretreatment washes (1-5 and 10 washes). Besides saccharification and co-fermentation data, the inhibitory effect of ChOAc on the biocatalyst was compared with that of 1-ethyl-3-methylimidazolium acetate (EmimOAc). ChOAc showed less inhibitory effect on cellulase and yeast activity compared with EmimOAc. The cellulose and hemicellulose saccharification percentages of the IL-pretreated bagasse were approximately 90% and 60%, respectively, irrespective of ChOAc and EmimOAc with sufficient post-pretreatment washes. The cellulose and hemicellulose saccharification percentages after 5 washes were 82% and 59%, respectively, in the case of ChOAc and 51% and 13%, respectively, in the case of EmimOAc. The overall ethanol yields on the original bagasse basis after saccharification and co-fermentation of the IL-pretreated bagasse when washed 5 times were 54% and 22% in the case of ChOAc and EmimOAc, respectively. ChOAc-pretreated bagasse could be saccharified and co-fermented with fewer washes than EmimOAc-pretreated bagasse.

    Elsevier, 2015年11月05日, Biochemical Engineering Journal, 103, 198 - 204, 英語

    研究論文(学術雑誌)

  • Shinji Hama, Chiaki Ogino, Akihiko Kondo

    Phospholipids (PLs) containing specific polar head groups and fatty acids, artificially synthesized from a complex mixture of natural PLs, have considerable industrial applications. The biocatalytic approaches to synthesizing structured PLs are of great interest because the enzymes used show high selectivity and performance under mild conditions, leading to the generation of products that cannot easily be obtained by chemical catalysis. Although the limited supply of phospholipases (e.g., phospholipase D) has thus far been an obstacle to the widespread use of enzymatic processing, recent advances in enzyme preparation have opened up various applications for PL modification. In this review, attempts to increase the productivity and utility of microbial phospholipases and lipases are presented. We also summarize recent developments in enzyme-catalyzed modification of PLs, focusing particularly on the relevant reactions, bioreactor design, and novel proof-of-concept experiments.

    SPRINGER, 2015年10月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99 (19), 7879 - 7891, 英語

    [査読有り]

    研究論文(学術雑誌)

  • バイオリファイナリーの現状と展望

    蓮沼 誠久, 石井 純, 荻野 千秋, 近藤 昭彦

    2015年10月, 化学と生物, 53 (10), 689 - 695, 日本語

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Sayuri Omote, Chiaki Ogino, Kosuke Kuroda, Mana Noguchi, Takatsugu Endo, Ryohei Kakuchi, Nobuaki Shimizu, Kenji Takahashi

    Choline acetate (ChOAc), a cholinium ionic liquid (IL), was compared with 1-ethyl-3-methylimidazolium acetate (EmimOAc) with regard to biomass pretreatment, inhibition on cellulase and yeast, residuals in pretreated biomass, and saccharification and fermentation of pretreated biomass. Irrespective of ChOAc and EmimOAc, cellulose and hemicellulose saccharification of the IL-pretreated bagasse were over 90% and 60%, respectively. Median effective concentrations (EC50) based on cellulase activity were 32wt% and 16wt% for ChOAc and EmimOAc, respectively. The EC50 based on yeast growth were 3.1wt% and 0.3wt% for ChOAc and EmimOAc respectively. The residuals in IL-pretreated bagasse were 10% and 23% for ChOAc and EmimOAc, respectively, when washed 2 times after pretreatment. Ethanol yield on a bagasse basis were 60% and 24% for ChOAc and EmimOAc, respectively, in the saccharification and fermentation of IL-pretreated bagasse when washed 2 times. ChOAc-pretreated bagasse could be saccharified and fermented with fewer wash times than EmimOAc-pretreated bagasse.

    Elsevier Ltd, 2015年08月01日, Bioresource Technology, 189, 203 - 209, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Hama, Shino Mizuno, Maki Kihara, Tsutomu Tanaka, Chiaki Ogino, Hideo Noda, Akihiko Kondo

    This study focused on the process development for the D-lactic acid production from cellulosic feedstocks using the Lactobacillus plantarum mutant, genetically modified to produce optically pure D-lactic acid from both glucose and xylose. The simultaneous saccharification and fermentation (SSF) using delignified hardwood pulp (5-15% loads) resulted in the lactic acid titers of 55.2-84.6 g/L after 72 h and increased productivities of 1.77-2.61 g/L/h. To facilitate the enzymatic saccharification of high-load pulp at a fermentation temperature, short-term (610 min) pulverization of pulp was conducted, leading to a significantly improved saccharification with the suppressed formation of formic acid by-product. The short-term milling followed by SSF resulted in a lactic acid titer of 102.3 g/L, an optical purity of 99.2%, and a yield of 0.879 g/g-sugars without fed-batch process control. Therefore, the process presented here shows promise for the production of high-titer D-lactic acid using the L. plantarum mutant. (C) 2015 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2015年07月, BIORESOURCE TECHNOLOGY, 187, 167 - 172, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Shimpei Aikawa, Fumio Matsuda, Mami Okamoto, Tomokazu Shirai, Hideo Kawaguchi, Chiaki Ogino, Masanori Yamasaki, Jun Kikuchi, Akihiko Kondo

    A renewable raw material, rice straw is pretreated for biorefinery usage. Solution-state two-dimensional (2D) H-1-(13) C hetero-nuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy, was used to analyze 13 cultivars of rice straw before and after dilute acid pretreatment, to characterize general changes in the lignin and polysaccharide components. Intensities of most (15 of 16) peaks related to lignin aromatic regions, such as p-coumarate, guaiacyl, syringyl, p-hydroxyphenyl, and cinnamyl alcohol, and methoxyl, increased or remained unchanged after pretreatment. In contrast, intensities of most (11 of 13) peaks related to lignin aliphatic linkages or ferulate decreased. Decreased heterogeneity in the intensities of three peaks related to cellulose components in acid-insoluble residues resulted in similar glucose yield (0.45-0.59 g/g-dry biomass). Starch-derived components showed positive correlations (r = 0.71 to 0.96) with glucose, 5-hydroxymethyl-furfural (5-HMF), and formate concentrations in the liquid hydrolysates, and negative correlations (r = -0.95 to -0.97) with xylose concentration and acid-insoluble residue yield. These results showed the fate of lignin and polysaccharide components by pretreatment, suggesting that lignin aromatic regions and cellulose components were retained in the acid insoluble residues and starch-derived components were transformed into glucose, 5-HMF, and formate in the liquid hydrolysate.

    PUBLIC LIBRARY SCIENCE, 2015年06月, PLOS ONE, 10 (6), e0128417, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryosuke Yamada, Toshihide Yoshie, Shoji Sakai, Satoshi Wakai, Nanami Asai-Nakashima, Fumiyoshi Okazaki, Chiaki Ogino, Hiromoto Hisada, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

    Kraft pulp is a promising feedstock for bioproduction. The efficiency of kraft pulp saccharification was improved by using a cellulase cocktail prepared from genetically engineered Aspergillus oryzae. Application of the cellulase cocktail was demonstrated by simultaneous saccharification and fermentation, using kraft pulp and non-cellulolytic yeast. Such application would make possible to do an efficient production of other chemicals from kraft pulp.

    TAYLOR & FRANCIS LTD, 2015年06月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79 (6), 1034 - 1037, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation (ultrafiltration permeation and nanofiltration concentration) to increase sugar contents, was investigated. Ethanol production at 5th batch fermentation by Saccharomyces cerevisiae BY4741 attained 113.7 +/- 3.1 g L-1 (89.1 +/- 2.2% of the theoretical ethanol yield) from 270.0 +/- 22.6 g L-1 sugars, corresponding to 98.7% of ethanol titer attained at the 1st batch fermentation. This titer was comparable to ethanol production of 115.8 +/- 0.6 g L-1 (87.1 +/- 2.7% of the theoretical ethanol yield) obtained at 5th batch fermentation with 3 g L-1 yeast extract and 6 g L-1 polypeptone. Increase of cell density in the concentrated sweet sorghum juice was observed during sequential batch fermentation, as indicated by increased OD600. Utilization of sweet sorghum juice as the sole source, membrane separation, and S. cerevisiae was a cost-effective process for high ethanol production. (C) 2015 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2015年06月, BIORESOURCE TECHNOLOGY, 186, 351 - 355, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Hiroshi Teramura, Daisuke Sasaki, Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

    Background: Hydrothermal pretreatment of lignocellulosic biomass such as rice straw can dissolve part of the lignin and hemicellulose into a liquid fraction, thus facilitating enzyme accessibility to cellulose in bioethanol production process. Lignin is awaited to be recovered after hydrothermal pretreatment for utilization as value-added chemical, and lignin recovery also means removal of fermentation inhibitors. To recover lignin with high content from the liquid fraction, it is necessary to separate lignin and hemicellulose-derived polysaccharide. Therefore, the following processes were applied: membrane separation with nanofiltration (NF) and enzymatic hydrolysis by hemicellulase. To clarify lignin-concentrated fraction obtained during these processes, the fates of lignin and polysaccharide components were pursued by a solution NMR method and confirmed by compositional analysis of each fraction. Results: After hydrothermal pretreatment of rice straw, the NF concentrate of the supernatant of liquid fraction was hydrolyzed by hemicellulase and the resulting black precipitate was recovered. In this black precipitate, the intensity of NMR spectra related to lignin aromatic regions increased and those related to polysaccharides decreased, compared to rice straw, the solid fraction after hydrothermal pretreatment, and the NF concentrate. The lignin content of the black precipitate was 65.8 %. Lignin in the black precipitate included 52.9 % of the acid-insoluble lignin and 19.4 % of the soluble lignin in the NF concentrate of supernatant of liquid fraction. Conclusion: A precipitate with high lignin content was obtained from supernatants of the liquid fraction. These results suggested that precipitation of lignin was enhanced from concentrated mixtures of lignin and hemicellulosic polysaccharides by hydrolyzing the polysaccharides. Precipitation of lignin can contribute to lignin recovery from lignocellulosic biomass and, at the same time, allow more efficient ethanol production in the subsequent fermentation process.

    BIOMED CENTRAL LTD, 2015年06月, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    Mechanical milling and membrane separation were applied to simultaneous saccharification and co-fermentation from hydrothermally pretreated rice straw. Mechanical milling with minimized 4 cycles enabled 37.5 +/- 3.4 g L-1 and 45.3 +/- 4.4 g L-1 of ethanol production after 48 h by xylose-fermenting Saccharomyces cerevisiae from solid fractions (200 and 250 g L-1) of pretreated rice straw with 5 filter paper unit g-biomass(-1) cellulase (respectively, 77.3 +/- 7.1% and 74.7 +/- 7.3% of theoretical ethanol yield). Use of a membrane-based process including nanofiltration and ultrafiltration increased the sugar concentrations in the liquid fraction of pretreated rice straw and addition of this liquid fraction to 250 g L-1 solid fraction increased ethanol production to 52.0 +/- 0.4 g L-1 (73.8 +/- 0.6% of theoretical ethanol yield). Mechanical milling was effective in increasing enzymatic hydrolysis of the solid fraction and membrane separation steps increased the ethanol titer during co-fermentation, leading to a proposal for combining these processes for ethanol production from whole rice straw. (C) 2015 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2015年06月, BIORESOURCE TECHNOLOGY, 185, 263 - 268, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, Akihiko Kondo

    Dilute acid-pretreated sorghum bagasse, which was predominantly composed of glucan (59%) and xylose (7.2%), was used as a lignocellulosic feedstock for D-phenyllactic acid (PhLA) production by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. During fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, the PhLA yield was reduced by 35% compared to filter paper hydrolysate, and metabolomics analysis revealed that NAD(P)H regeneration and intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate for PhLA biosynthesis markedly reduced. Compared to separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components, including p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual enzymatic hydrolysis during SSF enhances PhLA production under glucose limitation and reduces the accumulation of fermentation inhibitors, collectively leading to increased PhLA yield. (c) 2015 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2015年04月, BIORESOURCE TECHNOLOGY, 182, 169 - 178, 英語

    [査読有り][招待有り]

    研究論文(学術雑誌)

  • Izzat Fahimuddin Bin Mohamed Suffian, Yuya Nishimura, Kenta Morita, Sachiko Nakamura-Tsuruta, Khuloud T. Al-Jamal, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    Background: The hepatitis B virus core (HBc) particle is known as a promising new carrier for the delivery of drugs and nucleic acids. However, since the arginine-rich domain that is located in the C-terminal region of the HBc monomer binds to the heparan sulphate proteoglycan on the cell surface due to its positive charge, HBc particles are introduced non-specifically into a wide range of cells. To avoid non-specific cellular uptake with the intent to control the ability of cell targeting, we individually replaced the respective arginine (R) residues of the arginine-rich domain located in amino acid positions 150-159 in glycine (G) residues. Results: The mutated HBc particles in which R154 was replaced with glycine (G) residue (R154G) showed a drastic decrease in the ability to bind to the heparan sulphate proteoglycan and to avoid non-specific cellular uptake by several types of cancer cells. Conclusions: Because this mutant particle retains most of its C-terminal arginine-rich residues, it would be useful in the targeting of specificity-altered HBc particles in the delivery of nucleic acids.

    BIOMED CENTRAL LTD, 2015年02月, JOURNAL OF NANOBIOTECHNOLOGY, 13 (1), 15, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sarvesh Kumar Srivastava, Chiaki Ogino, Akihiko Kondo

    Biological synthesis of nanoparticles has been present in living organisms over the course of evolution to serve a variety of purposes. In this chapter, we discuss the latest trends and application for nanoparticle synthesis via plants, algae, yeast, bacteria, fungi, etc. There exists several review articles among others documenting studies about various biogenic sources and associated nanoparticle synthesis we have rather emphasized on recent research works which probed into novel applications of these bio-nanoparticles along with some important historical fi ndings. Also, we have discussed the challenges faced by biogenic methods along with possible areas to tweak in order to standardize this synthesis technique. Biogenic synthesis of nanoparticles has the potential to provide cost-effective, eco-friendly alternative to work as “biological nanofactories”/functionalization method once the attention has been shifted to understand the underlying mechanism, its in vitro replication and obtaining shape/size control over the nanoparticles being synthesized.

    Springer International Publishing, 2015年01月01日, Green Processes for Nanotechnology: From Inorganic to Bioinspired Nanomaterials, 237 - 257, 英語

    [査読有り]

    論文集(書籍)内論文

  • Kazuaki Ninomiya, Asami Kohori, Mai Tatsumi, Koji Osawa, Takatsugu Endo, Ryohei Kakuchi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

    Choline acetate (ChOAc), a cholinium ionic liquid (IL), showed almost the same bagasse pretreatment capability as 1-ethyl-3-methylimidazolium acetate (EmimOAc), a conventional imidazolium IL used for biomass pretreatment. Moreover, ChOAc showed less of an inhibitory effect on cellulase than EmimOAc. Thus, ChOAc was used for IL/ultrasound-assisted pretreatment and in situ enzymatic saccharification, where IL was not washed out from the pretreated bagasse but diluted with the addition of a buffer solution. When in situ saccharification was performed for 48 h in the presence of 10% ChOAc, the cellulose and hemicellulose saccharification percentages were 80% and 72%, respectively. When ChOAc was increased to 20%, the saccharification percentages were 72% and 53%, respectively. However, the values were just 28% and 2%, respectively, in case of 20% EmimOAc. A glucose/xylose solution free from IL and ChOAc aqueous solution without these sugars could be recovered separately by electrodialysis of the hydrolysate of in situ saccharification. (C) 2014 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2015年01月, BIORESOURCE TECHNOLOGY, 176, 169 - 174, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kudou M, Okazaki Fumiyoshi, Asai-Nakashima N, Ogino Chiaki, Kondo Akihiko

    Cold-adapted beta-1,3-xylanase (P.t.Xyn26A) from the psychrotrophic bacterium, Psychroflexus torquis, was expressed as a fusion protein with tandem repeats of the N-terminal domain of Protein S from Myxocuccus xanthus (ProS2) in Escherichia coli. After cell lysis in phosphate buffer, most of the ProS2-P.t.Xyn26A was located in the insoluble fraction and aggregated during purification. Arginine hydrochloride (ArgHCl) efficiently solubilized the ProS2-P.t.Xyn26A. The solubilized ProS2-P.t.Xyn26A was purified using immobilized metal affinity chromatography (IMAC) with 500 mM ArgHCl. After cleavage of ProS2-P.t.Xyn26A by human rhinovirus 3C protease, we confirmed that recombinant P.t.Xyn26A maintained its native fold. This is the first report of the expression of a cold-adapted enzyme fused with a ProS2 tag under IMAC purification using a high concentration of ArgHCl. These insights into the expression and purification should be useful during the handling of cold-adapted enzymes.

    SPRINGER, 2015年01月, Biotechnology Letters, 37 (1), 89 - 94, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Ken Inoue, Yuka Aomori, Ayaka Ohnishi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

    Choline acetate, a totally bio-derived ionic liquid (IL), was used for IL-assisted pretreatment and subsequent fractionation of bagasse into carbohydrate and lignin. The fractionated carbohydrate-rich material (CRM) and lignin-rich material (LRM) were 79.6% and 5.2% of the original bagasse, respectively. CRM contained nearly all carbohydrate and approximately 50% of lignin in the original bagasse. In addition, approximately 20% of lignin in the original bagasse was fractionated as LRM. The cellulose saccharification percentage was over 95% for CRM, although the value was only 20% for the original bagasse. The average molecular weight of LRM was 4.2 x 10(4), which was approximately ten-times lower than that of the hydrolysis residue of CRM. After five cycles of the processes, the recovery of choline acetate was 80% of the fresh one. Throughout the five cycles, choline acetate maintained its chemical structure and exhibited its pretreatment capability for fractionation and enzymatic hydrolysis of bagasse. (C) 2014 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2015年01月, CHEMICAL ENGINEERING JOURNAL, 259, 323 - 329, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 二酸化チタン粒子の生物学的応用

    荻野 千秋

    2015年01月, 化学と生物, 53 (1), 9 - 11, 日本語

    研究論文(学術雑誌)

  • KUDOU Motonori, OKAZAKI F, ASAI-NAKASHIMA N, OGINO Chiaki, KONDO Akihiko

    Cold-adapted beta-1,3-xylanase (P.t.Xyn26A) from the psychrotrophic bacterium, Psychroflexus torquis, was expressed as a fusion protein with tandem repeats of the N-terminal domain of Protein S from Myxocuccus xanthus (ProS2) in Escherichia coli. After cell lysis in phosphate buffer, most of the ProS2-P.t.Xyn26A was located in the insoluble fraction and aggregated during purification. Arginine hydrochloride (ArgHCl) efficiently solubilized the ProS2-P.t.Xyn26A. The solubilized ProS2-P.t.Xyn26A was purified using immobilized metal affinity chromatography (IMAC) with 500 mM ArgHCl. After cleavage of ProS2-P.t.Xyn26A by human rhinovirus 3C protease, we confirmed that recombinant P.t.Xyn26A maintained its native fold. This is the first report of the expression of a cold-adapted enzyme fused with a ProS2 tag under IMAC purification using a high concentration of ArgHCl. These insights into the expression and purification should be useful during the handling of cold-adapted enzymes.

    SPRINGER, 2015年01月, Biotechnology Letters, 37 (1), 89 - 94, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Saccharification and ethanol fermentation from cholinium ionic liquid-pretreated bagasse with a different number of post-pretreatment washings

    Ninomiya Kazuaki, Omote S, Ogino Chiaki, Kuroda K, Noguchi M, Endo T, Kakuchi R, Shimizu N, Takahashi K

    2015年, Bioresourse Technology, 6, 888, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Green Processes for Nanotechnology, Chapter 8, Nanoparticle Synthesis

    Srivastave S.K, Ogino Chiaki, 近藤 昭彦

    2015年, Biogenic Approach, Springer, 237 - 257, 日本語

    研究論文(学術雑誌)

  • Musashi Takenaka, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    An atomic force microscope (AFM) can measure the adhesion force between a sample and a cantilever while simultaneously applying a rupture force during the imaging of a sample. An AFM should be useful in targeting specific proteins on a cell surface. The present study proposes the use of an AFM to measure the adhesion force between targeting receptors and their ligands, and to map the targeting receptors. In this study, Ste2p, one of the G protein-coupled receptors (GPCRs), was chosen as the target receptor. The specific force between Ste2p on a yeast cell surface and a cantilever modified with its ligand, a-factor, was measured and found to be approximately 250 pN. In addition, through continuous measuring of the cell surface, a mapping of the receptors on the cell surface could be performed, which indicated the differences in the Ste2p expression levels. Therefore, the proposed AFM system is accurate for cell diagnosis.

    ROYAL SOC CHEMISTRY, 2015年, Nanoscale, 7 (11), 4956 - 4963, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoshi Wakai, Toshihide Yoshie, Nanami Asai-Nakashima, Ryosuke Yamada, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

    Lactic acid is a commodity chemical that can be produced biologically. Lactic acid-producing Aspergillus oryzae strains were constructed by genetic engineering. The A. oryzae LDH strain with the bovine L-lactate dehydrogenase gene produced 38 g/L of lactate from 100 g/L of glucose. Disruption of the wild-type lactate dehydrogenase gene in A. oryzae LDH improved lactate production. The resulting strain A. oryzae LDHD871 produced 49 g/L of lactate from 100 g/L of glucose. Because A. oryzae strains innately secrete amylases, A. oryzae LDHD871 produced approximately 30 g/L of lactate from various starches, dextrin, or maltose (all at 100 g/L). To our knowledge, this is the first report describing the simultaneous saccharification and fermentation of lactate from starch using a pure culture of transgenic A. oryzae. Our results indicate that A. oryzae could be a promising host for the bioproduction of useful compounds such as lactic acid. (C) 2014 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2014年12月, BIORESOURCE TECHNOLOGY, 173, 376 - 383, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kiyotaka Y. Hara, Toshihiko Morita, Masao Mochizuki, Keisuke Yamamoto, Chiaki Ogino, Michihiro Araki, Akihiko Kondo

    Background: Red yeast, Xanthophyllomyces dendrorhous (Phaffia rhodozyma) is the only yeast known to produce astaxanthin, an anti-oxidant isoprenoid (carotenoid) that is widely used in the aquaculture, food, pharmaceutical and cosmetic industries. Recently, the potential of this microorganism as a platform cell factory for isoprenoid production has been recognized because of high flux through its native terpene pathway. Addition of mevalonate, the common precursor for isoprenoid biosynthesis, has been shown to be critical to enhance the astaxanthin content in X. dendrorhous. However, addition of mevalonate is unrealistic during industrial isoprenoid production because it is an unstable and costly chemical. Therefore, up-regulating the intracellular mevalonate supply by enhancing the mevalonate synthetic pathway though genetic engineering is a promising strategy to improve isoprenoid production in X. dendrorhous. However, a system to strongly express multiple genes has been poorly developed for X. dendrorhous. Results: Here, we developed a multiple gene expression system using plasmids containing three strong promoters in X. dendrorhous (actin, alcohol dehydrogenase and triose-phosphate isomerase) and their terminators. Using this system, three mevalonate synthetic pathway genes encoding acetoacetyl-CoA thiolase, HMG-CoA synthase and HMG CoA reductase were overexpressed at the same time. This triple overexpressing strain showed an increase in astaxanthin production compared with each single overexpressing strain. Additionally, this triple overexpression of mevalonate synthetic pathway genes together with genes involved in beta-carotene and astaxanthin synthesis showed a synergetic effect on increasing astaxanthin production. Finally, astaxanthin production was enhanced by 2.1-fold compared with the parental strain without a reduction of cell growth. Conclusions: We developed a system to strongly overexpress multiple genes in X. dendrorhous. Using this system, the synthetic pathway of mevalonate, a common substrate for isoprenoid biosynthesis, was enhanced, causing an increase in astaxanthin production. Combining this multiple gene overexpression system with a platform strain that overproduces mevalonate has the potential to improve industrial production of various isoprenoids in X. dendrorhous.

    BIOMED CENTRAL LTD, 2014年12月, MICROBIAL CELL FACTORIES, 13 (1), 175, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Norimasa Kashiwagi, Michiru Miyake, Shuichi Hirose, Masahiro Sota, Chiaki Ogino, Akihiko Kondo

    The end products from starch hydrolysis by amylases have important applications in various industries. Here, two amylases derived from two Streptomyces species that hydrolyze soluble starch from potato produced maltotriose as the primary maltooligosaccharide product. The genes, annotated as putative glycoside hydrolases, were cloned and expressed in Streptomyces lividans. These amylases displayed hydrolysis activity from pH 3 to 9.5 and were not affected by Ca2+. Optimal production of maltotriose was between 20 and 30 A degrees C at pH 6.5. At the optimal temperature, both amylases produced maltotriose-rich end products rather than either maltose or maltotetraose.

    SPRINGER, 2014年11月, BIOTECHNOLOGY LETTERS, 36 (11), 2311 - 2317, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo

    Oligomeric sugars in the liquid fraction of hot water-pretreated rice straw are more amenable to membrane process than monomeric sugars, as lower pressure is required. Following membrane process was employed: nanofiltration (NF) concentration -> (dilution -> NF concentration) x 2 times -> enzymatic hydrolysis (EH) -> ultrafiltration (UF) permeation [Implication: NF for recovery of oligomeric sugars, dilution and NF for removal of low molecular weight fermentation inhibitors, UF for removal of high molecular weight fermentation inhibitors and recovery of monomeric sugars after EH]. This process provided the liquid fraction containing 111.4 g L-1 of sugars, corresponding to 681.0 mM as monomeric sugars, from the original liquid fraction (181.1 mM monomeric sugars). Concentrations of low molecular weight fermentation inhibitors, acetic and formic acids, were decreased to 24% and 48%, respectively. Xylose-fermenting recombinant Saccharomyces cerevisiae produced 34.5 +/- 2.2 g L-1 ethanol from the 0.8 times liquid fraction (76% of theoretical yield). (C) 2014 Published by Elsevier Ltd.

    ELSEVIER SCI LTD, 2014年10月, BIORESOURCE TECHNOLOGY, 169, 380 - 386, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Satoshi Wakai, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

    The aim of this investigation was to attain high ethanol concentration by concentrating sweet sorghum juice using a two-step membrane separation process. Ultrafiltration permeation of the juice was used to remove residues, followed by nanofiltration concentration to increase the sugar concentration. The concentrated juice containing 180.0 g L-1 sucrose, 59.3 g L-1 glucose and 49.3 g L-1 fructose supplemented with nitrogen sources (10 and 20 g L-1 of yeast extract and polypeptone, respectively) was fermented by Saccharomyces cerevisiae BY4741 to produce 133.5 g L-1 of ethanol (87.6% of theoretical yield) after 48 h fermentation. Importantly, the addition of lower concentrations of exogenous nitrogen sources (3 and 6 g L-1 of yeast extract and polypeptone, respectively) or no exogenous nitrogen sources resulted in the production of 131.4 and 132.8 g L-1 of ethanol (84.8% and 86.0% of theoretical yield), respectively, after 48 h fermentation. (C) 2014 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2014年10月, BIORESOURCE TECHNOLOGY, 169, 821 - 825, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Aya Fukuda, Chiaki Ogino, Nobuaki Shimizu

    In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm(2), 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24 h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h. (C) 2014 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2014年09月, ULTRASONICS SONOCHEMISTRY, 21 (5), 1624 - 1628, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Takashi Yamauchi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

    We demonstrated that the enzymatic hydrolysis of cellulose after microwave pretreatment of lignocellulosic material in ionic liquids (ILs) is drastically enhanced compared with that after conventional thermal pretreatment in ILs. Three types of cholinium ILs, choline formate (ChFor), choline acetate (ChOAc), and choline propionate (ChPro), were examined. The cellulose saccharification percentage was approximately 20% for kenaf powders pretreated in ChFor, ChOAc, and ChPro by conventional heating at 110 degrees C for 20 min. In contrast, approximately 60-90% of cellulose was hydrolyzed to glucose after microwave pretreatment in the same ILs at 110 degrees C for 20 min. (C) 2014 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2014年09月, BIOCHEMICAL ENGINEERING JOURNAL, 90, 90 - 95, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    Techniques using nanotechnology in the detection and treatment of cancers have made great progress in multidisciplinary fields. The advances in drug delivery systems (DDSs) have been supported mainly by the development of varied nanoparticles (NPs). Although the NPs based on organic and inorganic materials are integral parts in DDSs, bio-nanoparticles containing biopolymer and virus-like particles (VLPs) are attractive biomaterials for DDSs because of their unique features originating in bio-based materials, such as biocompatibility, biodegradability and low immunogenicity. It is notable that these NPs additionally have a great advantage to enable the easy and flexible alteration of their features by genetic engineering approaches. Controlling the sequence and oligomeric process of polypeptide genes permits a variety of choices in type or size of biopolymeric NPs (e.g., elastin-like polypeptide NPs). In contrast, the functional genes are often inserted into the coding sequences for self-assembled proteins to give the VLPs (e.g., hemagglutinating virus of Japan, adeno-associated virus, human immunodeficiency virus-1, simian virus 40 and hepatitis B virus) additional functions. Thus, genetic engineering readily allow alterations of the properties of NPs (e.g., particle shape, size and stability) and grant of new abilities (e.g., cell-specificity and drug loading and release). In this review, we introduce recent advances in bio-nanoparticles from the standpoint of engineering.

    AMER SCIENTIFIC PUBLISHERS, 2014年09月, JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 10 (9), 2063 - 2085, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuma Ogura, Kazuaki Ninomiya, Kenji Takahashi, Chiaki Ogino, Akihiko Kondo

    Background: Lignocellulosic biomass from plant biomass, especially softwoods, are well-known to present difficulties during attempts at hydrolysis due to their rigid structure. Pretreatment of lignocellulosic biomass with ionic liquids (ILs) is attractive as this requires to a low input of energy. However, IL pretreatment has the disadvantage of the presence of large amounts of water. Recently, it was reported that a small amount of acid has a positive effect on the degradation of biomass in IL with water. In this study the pretreatment of Japanese cedar, the most abundant softwood in Japan, was investigated using a combination of IL, acid and metal ions. Results: First, the novel ionic liquid pretreatment was investigated by changing the pretreatment solvent and the anti-solvent. A mixture of IL, acid and ferric oxide (Fe3+) ion was most effective for pretreatment, and an acetone-water mixture was also most effective on the precipitation of biomass. These optimized pretreatment combinations attained a higher degree of glucose release from the pretreated biomass. The amount of cellulose was concentrated from to a level of 36 to 84% of the insoluble fraction by the optimized pretreatment. Based on this result, it was assumed that the extraction of the lignin fraction from the biomass into an anti-solvent solution was attained. Finally, this optimized pretreatment was applied to the enzymatic hydrolysis of Japanese cedar at high-solid biomass loading, and 110 g/L of glucose production was attained. In addition, the ethanol fermentation with this hydrolyzed solution by Saccharomyces cerevisiae achieved 50 g/L ethanol production, and this yield reached 90% of the theoretical yield. Conclusions: We developed an effective pretreatment protocol by changing to a pretreatment solvent containing IL, acid, metal ion and anti-solvent. The optimized pretreatment has an effect on softwood and separately retrieved lignin as a by-product. The saccharified solution at high-solid biomass loading was converted to ethanol in a high yield. This proposed methodology would boost the performance of the bioconversion of low-cost materials to other chemicals, and would not be limited to only ethanol but also would include other target chemicals.

    BIOMED CENTRAL LTD, 2014年08月, BIOTECHNOLOGY FOR BIOFUELS, 7, 120, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiak Ogino, Tetsuya Matsuda, Fumiyoshi Okazaki, Tsutomu Tanaka, Akihiko Kondo

    Effective secretion of green fluorescent protein (GFP) was investigated by the screening signal sequences for GFP secretion in Aspergillus oryzae. GFP production in A. oryzae was evaluated using fusions with signal sequences from Taka-amylase A (TAA), glucoamylase A, glucoamylase S, and triacylglycerol lipase. The TAA signal sequence promoted the highest protein secretion of GFP. Fusing this signal sequence with an N-terminal 28-amino acid region (N28 fragment) from the Rhizopus oryzae lipase signal sequence increased protein secretion. In addition, using multiple copies of this signal sequence, instead of the N28 fragment, also induced protein secretion. These results show that using multiple signal sequences or combining a signal sequence with the N28 fragment can be used to improve heterogeneous protein secretion in A. oryzae. (C) 2014 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2014年07月, PROCESS BIOCHEMISTRY, 49 (7), 1078 - 1083, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Kawaguchi, Kouji Uematsu, Chiaki Ogino, Hiroshi Teramura, Satoko Niimi-Nakamura, Yota Tsuge, Tomohisa Hasunuma, Ken-Ichi Oinuma, Naoki Takaya, Akihiko Kondo

    Simultaneous saccharification and fermentation (SSF) of renewable cellulose for the production of 3-phenyllactic acid (PhLA) by recombinant Escherichia coli was investigated. Kraft pulp recovered from biomass fractionation processes was used as a model cellulosic feedstock and was hydrolyzed using 10-50 filter paper unit (FPU) g(-1) kraft pulp of a commercial cellulase mixture, which increased the glucose yield from 21% to 72% in an enzyme dose-dependent manner. PhLA fermentation of the hydrolyzed kraft pulp by a recombinant E. coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens TK1 produced 1.9 mM PhLA. The PhLA yield obtained using separate hydrolysis and fermentation was enhanced from 5.8% to 42% by process integration into SSF of kraft pulp (20 g L-1) in a complex medium (pH 7.0) at 37 degrees C The PhLA yield was negatively correlated with the initial glucose concentration, with a five-fold higher PhLA yield observed in culture medium containing log L-1 glucose compared to 100 g L-1. Taken together, these results suggest that the PhLA yield from cellulose in kraft pulp can be improved by SSF under glucose-limited conditions. (C) 2014 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2014年07月, BIOCHEMICAL ENGINEERING JOURNAL, 88, 188 - 194, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 「バイオプロダクション次世代農工連携研究」と拠点形成への展望

    近藤 昭彦, 荻野 千秋

    2014年06月, 紙パルプ技術タイムス, 57 (6), 51 - 56, 日本語

    研究論文(学術雑誌)

  • Motonori Kudou, Yuka Kubota, Nanami Nakashima, Fumiyoshi Okazaki, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

    The uses of ionic liquids (ILs) to alter the performance of enzymes and to pretreat cellulosic biomass have been investigated. In the present study, the activity of beta-glucosidase from Themotoga maritime (TmBgl1A) was studied in a phosphate buffer containing imidazolium-based ILs. Among the imidazolium-based ILs that were tested, 1-butyl-3-methylimidazolium acetate [Bmim][OAc] showed the most improvement for the hydrolysis activity of TmBgl1A at pH 7.0. From the steady-state emission spectra, [Bmim][OAc] quenched the intrinsic fluorescence of TmBgl1A. The improvement in the activity of TmBgl1A induced by [Bmim][OAc] could be linked to the flexibility of the conformation of TmBgl1A. (C) 2014 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2014年06月, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 104, 17 - 22, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Enhanced OH radical generation by dual-frequency ultrasound with TiO2nanoparticles: its appplication to targeted sonodynamic therapy

    NINOMIYA K, NODA K, OGINO Chiaki, KURODA S, SHIMIZU N

    2014年05月, Ultrasonic Sonochemistry, 21 (1), 289 - 294, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryosuke Yamada, Toshihide Yoshie, Satoshi Wakai, Nanami Asai-Nakashima, Fumiyoshi Okazaki, Chiaki Ogino, Hiromoto Hisada, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

    Background: Kojic acid (5-Hydroxy-2-(hydroxymethyl)-4-pyrone) is one of the major secondary metabolites in Aspergillus oryzae. It is widely used in food, pharmaceuticals, and cosmetics. The production cost, however, is too high for its use in many applications. Thus, an efficient and cost-effective kojic acid production process would be valuable. However, little is known about the complete set of genes for kojic acid production. Currently, kojic acid is produced from glucose. The efficient production of kojic acid using cellulose as an inexpensive substrate would help establish cost-effective kojic acid production. Results: A kojic acid transcription factor gene over-expressing the A. oryzae strain was constructed. Three genes related to kojic acid production in this strain were transcribed in higher amounts than those found in the wild-type strain. This strain produced 26.4 g/L kojic acid from 80 g/L glucose. Furthermore, this strain was transformed with plasmid harboring 3 cellulase genes. The resultant A. oryzae strain successfully produced 0.18 g/L of kojic acid in 6 days of fermentation from the phosphoric acid swollen cellulose. Conclusions: Kojic acid was produced directly from cellulose material using genetically engineered A. oryzae. Because A. oryzae has efficient protein secretion ability and secondary metabolite productivity, an A. oryzae-based cell factory could be a platform for the production of various kinds of bio-based chemicals.

    BIOMED CENTRAL LTD, 2014年05月, MICROBIAL CELL FACTORIES, 13, 71, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 植物の機能解明と新たな機能材料創出の可能性

    寺村 浩, 延田 紘治, 川口 秀夫, 荻野 千秋, 西野 孝, 近藤 昭彦

    2014年04月, バイオインダストリー, 31 (4), 62 - 68, 日本語

    研究論文(学術雑誌)

  • Naoko Okai, Chihiro Takahashi, Kazuki Hatada, Chiaki Ogino, Akihiko Kondo

    Gamma-aminobutyric acid (GABA), a building block of the biodegradable plastic polyamide 4, is synthesized from glucose by Corynebacterium glutamicum that expresses Escherichia coli glutamate decarboxylase (GAD) B encoded by gadB. This strain was engineered to produce GABA more efficiently from biomass-derived sugars. To enhance GABA production further by increasing the intracellular concentration of its precursor glutamate, we focused on engineering pknG (encoding serine/threonine protein kinase G), which controls the activity of 2-oxoglutarate dehydrogenase (Odh) in the tricarboxylic acid cycle branch point leading to glutamate synthesis. We succeeded in expressing GadB in a C. glutamicum strain harboring a deletion of pknG. C. glutamicum strains GAD and GAD Delta pknG were cultured in GP2 medium containing 100 g L-1 glucose and 0.1 mM pyridoxal 5'-phosphate. Strain GAD Delta pknG produced 31.1 +/- 0.41 g L-1 (0.259 g L-1 h(-1)) of GABA in 120 hours, representing a 2.29-fold higher level compared with GAD. The production yield of GABA from glucose by GAD Delta pknG reached 0.893 mol mol(-1).

    BIOMED CENTRAL LTD, 2014年04月, AMB EXPRESS, 4, 20, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Koichi Takeda, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    Background: An affibody-displaying bio-nanocapsule (Z(HER2)-BNC) with a hepatocyte specificity derived from hepatitis B virus (HBV) was converted into an affibody, Z(HER2), that recognizes HER2 receptors. This affibody was previously reported to be the result of the endocytosis-dependent specific uptake of proteins and siRNA into target cancer cells. To assist the endosomal escape of inclusions, a helper lipid with pH-sensitive fusogenic ability (1,2-dioleoyl-sn-glycero-3-phos phoethanolamine; DOPE) was conjugated with a Z(HER2)-BNC. Findings: In this study, we displayed a pH-sensitive fusogenic GALA peptide on the surface of a particle in order to confer the ability of endosomal escape to a Z(HER2)-BNC. A GALA-displaying Z(HER2)-BNC purified from yeast uneventfully formed a particle structure. Furthermore, endosomal escape of the particle was facilitated after endocytic uptake and release of the inclusions to the cytoplasm without the cell toxicity. Conclusion: The genetic fusion of a GALA peptide to the virus-like particle confers the ability of endosomal escape.

    BIOMED CENTRAL LTD, 2014年04月, JOURNAL OF NANOBIOTECHNOLOGY, 12 (1), 11, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Structural evaluation of the DNA aptamer for ATP DH25.42 by AFM., Nucleosides

    MIYACHI Y, OGINO Chiaki, KONDO Akihiko

    2014年02月, Nucleosides, Nucleotides and Nucleic Acids, 33 (1), 31 - 39, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jun Ishii, Asami Oda, Shota Togawa, Akira Fukao, Toshinobu Fujiwara, Chiaki Ogino, Akihiko Kondo

    Neurotensin receptor type-I (NTSR1) is a member of the G-protein-coupled receptor (GPCR) family. The natural ligand of NTSR1 is neurotensin (NT), a neuromodulator of the central nervous system. Because NT is also involved in many oncogenic actions, the signaling mediator NTSR1 is a significant molecular target in medicinal and therapeutic fields. In the current study, we constructed a fluorescence-based microbial yeast biosensor that can monitor the activation of human NTSR1 signaling responding to its agonist. To increase the sensitivity of the biosensor, a yeast strain with the green fluorescent protein (GFP) reporter gene was genetically engineered to enhance binding with human NTSRI expressed on the membrane. Following previous reports, the 5 carboxy-terminal amino acid residues of the guanine nucleotide binding protein a-subunit (G alpha) in yeast Gpalp were substituted with the equivalent human G alpha, sequences (Gpal/G alpha(q) transplant). After optimizing the assay conditions, the G alpha-engineered yeast demonstrated significantly improved sensing for NTSR1 signaling. Because detection using a GFP fluorescence reporter considerably simplifies the measurement procedure, this microbial fluorescence sensor holds promise for use in the diagnosis of NTSR1-associated diseases and the development of agonists. (C) 2013 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014年02月, Analytical Biochemistry, 446, 37 - 43, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Takaki, Yoshihiro Higuchi, Minako Hashii, Chiaki Ogino, Nobuaki Shimizu

    We investigated the effects of nanosized TiO2 particles on the death of mouse leukemia L1210 cells. TiO2 particles suppressed proliferation and induced cell death, as measured by lactate dehydrogenase (LDH) release into the culture medium. Chromatin condensation, which is typical of the initiation of cell death, was observed in approximately 14% cells cultured with titanium dioxide (TiO2) particles for 12 h. Furthermore, giant DNA fragments of approximately 2 Mbp and high-molecular-weight DNA fragments between 100 kbp and 1 Mbp were observed in cells cultured for 18 h with TiO2 particles. These giant and high-molecular-weight DNA fragments were further degraded into smaller DNA fragments, appearing as DNA ladders. Corresponding to the generation of DNA fragments, caspase-3 activity increased in cells treated with TiO2 particles. TiO2 particle-induced LDH release was not inhibited by cytochalasin D, an inhibitor of endocytosis. These results suggest that nanosized TiO2 particles can induce apoptosis associated with DNA fragmentation and caspase-3 activation and that TiO2 particle-induced apoptosis is not caused by endocytosis but is associated with contact of the particles with the cell surface. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2014年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117 (1), 129 - 133, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sarvesh Kumar Srivastava, Chiaki Ogino, Akihiko Kondo

    A novel green one-pot approach for surface modification of graphene oxide into organosulfur modified graphene nanosheets (OS-GNS) is being reported. Alliin (garlic phytochemical) mediated organothiol linkages over OS-GNS facilitated one-step attachment of pre-synthesized gold nanoparticles. In absence of alliin treatment, no Au NP attachment was observed.

    ROYAL SOC CHEMISTRY, 2014年, RSC ADVANCES, 4 (12), 5986 - 5989, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sarvesh Kumar Srivastava, Jonathan Sabate del Rio, Ciara K. O'Sullivan, Chiaki Ogino, Akihiko Kondo

    We report an eco-friendly, one-pot, room-temperature method for the rapid synthesis of electrocatalytically active Au@Pt (50 nm) bimetallic nanoparticles via a tryptophan (Trp) mediated supramolecular interface in an aqueous environment. Our results demonstrate a simple universal approach for high shell-metal loading where a pre-stabilized tryptophan polymerized-Au core serves as a template to facilitate subsequent deposition of Pt. We observed that the amine-stabilized poly-Trp bi-layer has an enhancing effect on the electrocatalytic potential of Au@Pt NPs by the virtue of an amine stabilized interface, thereby enhancing the HER activity over glassy carbon electrodes. Several characterization techniques were used to confirm the inherent core-shell morphology of the resulting Au@Pt NPs. This Trp mediated facile green synthesis strategy has the potential to synthesize an array of Au-core containing bimetallic nanoparticles with enhanced catalytic activity and stable structure integration.

    ROYAL SOC CHEMISTRY, 2014年, RSC ADVANCES, 4 (89), 48458 - 48464, 英語

    [査読有り]

    研究論文(学術雑誌)

  • HWANG SY, NAKASHIMA K, OKAI Naoko, OKAZAKI F, MIYAKE M, HANAZONO K, OGINO Chiaki, KONDO Akihiko

    Amylases from Streptontyces are useful in the production of maltooligosaccharides, but they have weak thermal stability at temperatures higher than 40 degrees C. In this study, alpha-amylase (SAV5981 gene of Streptomyces avermitilis) was expressed from Streptomyces lividans 1326 and purified by ammonium sulfate fractionation followed by anionic chromatography (Q-HP sepharose). The properties of the purified SAV5981 amylase were determined by the starch-iodine method. The effect of metal ions on amylase activity was investigated. The optimal temperature shifted from 25 to 50 degrees C with the addition of the Ca2+ ion. The thermal stability of SAV5981 was also dramatically enhanced by the addition of 10 mm CaCl2. Improvement of the thermal stability of SAV5981 was examined by CD spectra in the presence and the absence of the Ca2+ ion. Thin-layer chromatography (TLC) analysis and HPLC analysis of starch degradation revealed that SAV5981 mainly produced maltose and maltotriose, not glucose. The maltoorigosaccharide-producing amylase examined in this study has the potential in the industrial application of oligosaccharide production.

    TAYLOR & FRANCIS LTD, 2013年12月, Bioscience, Biotechnlolgy, and Biochemistry, 77 (12), 2449 - 2453, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Teramura, Tomoko Oshima, Fumio Matsuda, Kengo Sasaki, Chiaki Ogino, Masanori Yamasaki, Akihiko Kondo

    Lignocellulosic biomass, such as rice straw, is often utilized as a bioresource after being hydrolyzed using dilute acid and separated into liquid hydrolysate and acid-insoluble residue. However, the biomass component that determines the distribution between liquid hydrolysate and acid-insoluble residue has not yet been clarified. In this study, the glucose content in the liquid hydrolysate and weight of acid-insoluble residue of 13 rice cultivars were analyzed. Starch content was positively correlated with glucose content in the liquid hydrolysate, and negatively correlated with acid-insoluble residue weight. These results indicate that the glucose in the liquid hydrolysate is mainly liberated from starch rather than cellulose in the rice straw. These observations suggest that starch content is a good indicator of the glucose distribution between the liquid hydrolysate and insoluble residue. (C) 2013 Published by Elsevier Ltd.

    ELSEVIER SCI LTD, 2013年12月, BIORESOURCE TECHNOLOGY, 149, 520 - 524, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Z. Tan, K. Sato, S. Takami, C. Numako, M. Umetsu, K. Soga, M. Nakayama, R. Sasaki, T. Tanaka, C. Ogino, A. Kondo, K. Yamamoto, T. Hashishin, S. Ohara

    Size-controllable TiO 2 nanosheets with highly exposed {001} facets were synthesized by a hydrothermal method. The particle sizes ranged from 25 nm to submicrometres by carefully adjusting the F/Ti molar ratio. TiO 2 nanosheets smaller than 100 nm have higher photocatalytic activity and are highly stable in degradation of organic dyes. © 2013 The Royal Society of Chemistry.

    2013年11月14日, Royal Society of Chemistry Advances, 3 (42), 19268 - 19271

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    Concentrating sugars using membrane separation, followed by ethanol fermentation by recombinant xylose-assimilating Saccharomyces cerevisiae, is an attractive technology. Three nanofiltration membranes (NTR-729HF, NTR-7250, and ESNA3) were effective in concentrating glucose, fructose, and sucrose from dilute molasses solution and no permeation of sucrose. The separation factors of acetate, formate, furfural, and 5-hydroxymethyl furfural, which were produced by dilute acid pretreatment of rice straw, over glucose after passage through these three membranes were 3.37-11.22, 4.71-20.27, 4.32-16.45, and 4.05-16.84, respectively, at pH 5.0, an applied pressure of 1.5 or 2.0 MPa, and 25 degrees C. The separation factors of these fermentation inhibitors over xylose were infinite, as there was no permeation of xylose. Ethanol production from approximately two-times concentrated liquid hydrolysate using recombinant S. cerevisiae was double (5.34-6.44 g L-1) that compared with fermentation of liquid hydrolysate before membrane separation (2.75 g L-1). (C) 2013 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2013年11月, BIORESOURCE TECHNOLOGY, 147, 84 - 88, 英語

    [査読有り]

    研究論文(学術雑誌)

  • ナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発

    田野 亜都里, 森田 健太, 荻野 千秋, 佐藤 和好, 沼子 千弥, 中山 雅央, 佐々木 良平, 近藤 昭彦

    2013年08月, 日本生物工学会大会講演要旨集平成25年度, 244, 日本語

    研究論文(その他学術会議資料等)

  • Kazuaki Ninomiya, Chiaki Ogino, Shinya Kawabata, Kentaro Kitamura, Teruya Maki, Hiroshi Hasegawa, Nobuaki Shimizu

    This is the first study to demonstrate that Microcystis aeruginosa, a typical algal bloom-forming cyanobacterium, can be effectively inactivated by ultrasound (US) irradiation in the presence of titanium dioxide (TiO2) particles as a sonocatalyst. When a culture broth of M. aeruginosa was ultrasonically irradiated for 15 min in the presence of 0.5 g/mL of TiO2 particles 2 mm in diameter, the cell survival ratio was 0.13, which was significantly lower than that in the case of US irradiation alone (0.87). Moreover, regrowth of M. aeruginosa in the culture was also inhibited for 10 days following ultrasonic disinfection in the presence of TiO2 particles for 15 min. (c) 2013, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2013年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116 (2), 214 - 218, 英語

    [査読有り]

    研究論文(学術雑誌)

  • OKAZAKI F, NAKASHIMA N, OGINO Chiaki, KONDO Akihiko

    The biochemical properties of a putative beta-1,3-xylanase from the hyperthermophilic eubacterium Thermotoga neapolitana DSM 4359 were determined from a recombinant protein (TnXyn26A) expressed in Escherichia coli. This enzyme showed specific hydrolytic activity against beta-1,3-xylan and released beta-1,3-xylobiose and beta-1,3-xylotriose as main products. It displayed maximum activity at 85 A degrees C during a 10-min incubation, and its activity half-life was 23.9 h at 85 A degrees C. Enzyme activity was stable in the pH range 3-10, with pH 6.5 being optimal. Enzyme activity was significantly inhibited by the presence of N-bromosuccinimide (NBS). The insoluble beta-1,3-xylan K (m) value was 10.35 mg/ml and the k (cat) value was 588.24 s(-1). The observed high thermostability and catalytic efficiency of TnXyn26A is both industrially desirable and also aids an understanding of the chemistry of its hydrolytic reaction.

    SPRINGER, 2013年08月, Applied Microbiology and Biotechnology, 97 (15), 6749 - 6757, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Hiroaki Mieda, Jun Ishii, Chiaki Ogino, Toshinobu Fujiwara, Akihiko Kondo

    Background: Small interfering RNA (siRNA) has attracted attention in the field of nucleic acid medicine as a RNA interference (RNAi) application that leads to gene silencing due to specific messenger RNA (mRNA) destruction. However, since siRNA is unstable in blood and unable to cross the cell membrane, encapsulation of siRNA into a carrier is required. Results: In this study, we used a carrier that combined Z(HER2)-displaying bio-nanocapsule (derived from hepatitis B virus surface antigen) and liposomes in a complex in order to investigate the feasibility of effective and target-cell-specific RNAi applications. As a result, by observing RNAi only in HER2-expressing breast cancer cells, using our proposed methodology, we successfully demonstrated target-cell-specific delivery and effective function expression of siRNA. Conclusions: These findings show that, in the field of nucleic acid medicine, Z(HER2)-BNC/ LP can be a useful carrier for siRNA delivery, and could also become a useful tool for gene silencing and to accomplish protein knock-down.

    BIOMED CENTRAL LTD, 2013年06月, JOURNAL OF NANOBIOTECHNOLOGY, 11, 19, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose

    NAKATANI Y, YAMADA R, OGINO Chiaki, KONDO Akihiko

    2013年06月, Microbial Cell Factories, 12 (1), 66, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yoshifumi Kawai, Shuhei Noda, Chiaki Ogino, Yasunobu Takeshima, Naoko Okai, Tsutomu Tanaka, Akihiko Kondo

    Background: p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmful to the environment. Although pHCA production using microbes has been widely studied, there remains a need for more cost-effective methods, such as the use of biomass as a carbon source. In this study, we produced pHCA using tyrosine ammonia lyase-expressing Streptomyces lividans. In order to improve pHCA productivity from cellulose, we constructed a tyrosine ammonia lyase- and endoglucanase (EG)-expressing S. lividans transformant and used it to produce pHCA from cellulose.Results: A Streptomyces lividans transformant was constructed to express tyrosine ammonia lyase derived from Rhodobacter sphaeroides (RsTAL). The transformant produced 786 or 736 mg/L of pHCA after 7 days of cultivation in medium containing 1% glucose or cellobiose as the carbon source, respectively. To enhance pHCA production from phosphoric acid swollen cellulose (PASC), we introduced the gene encoding EG into RsTAL-expressing S. lividans. After 7 days of cultivation, this transformant produced 753, 743, or 500 mg/L of pHCA from 1% glucose, cellobiose, or PASC, respectively.Conclusions: RsTAL-expressing S. lividans can produce pHCA from glucose and cellobiose. Similarly, RsTAL- and EG-expressing S. lividans can produce pHCA from glucose and cellobiose with excess EG activity remaining in the supernatant. This transformant demonstrated improved pHCA production from cellulose. Further enhancements in the cellulose degradation capability of the transformant will be necessary in order to achieve further improvements in pHCA production from cellulose. © 2013 Kawai et al. licensee BioMed Central Ltd.

    2013年05月07日, Microbial Cell Factories, 12 (1), 45, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Ogino Chiaki, Akihiko Kondo

    Streptomyces mobaraensis transglutaminase (MTG) is one of the most useful transglutaminases due to its rather broad substrate specificity and independence of Ca2+. To achieve efficient production of active-form MTG using Streptomyces lividans as a host, we created three vector constructs consisting of the signal peptide sequence (pld signal) derived from the phospholipase D gene of Streptomyces cinnamoneus, prepro-domain of S. cinnamoneus transglutaminase, and the sequence encoding mature MTG, and then generated three over-expressing S. lividans strains. We successfully demonstrated that S. lividans can be used as a host for the efficient production of mature, active-form MTG. © 2013 Elsevier B.V.

    2013年05月05日, Biochemical Engineering Journal, 74, 76 - 80, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sarvesh Kumar Srivastava, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

    Sidewall modification of multiwalled carbon nanotubes (abbreviated as MWCNTs) was achieved using Allivum sativum (garlic) extract by an acid-free green process. These organosulfur modified-MWCNTs were then decorated with gold nanoparticles and examined by transmission electron microscopy. The presence of organosulfurs over the modified nanotube surface was confirmed. Nanotube surface modification and subsequent presence of thiols as an active linker was confirmed by Raman spectroscopy, Fourier transform infrared spectroscopy, energy dispersive X-ray and X-ray photoelectron spectroscopy. In the absence of these organosulfurs (thiols), no gold nanoparticle attachment was observed. Both small (1-8 nm) and large (12-20 nm) gold nanoparticles were found to decorate the modified nanotube surface suggesting coalescence among nanoparticles. (C) 2013 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2013年05月, CARBON, 56, 309 - 316, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Adachi, Shinji Hama, Kazunori Nakashima, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

    For enzymatic biodiesel production from plant oil hydrolysates, an Aspergillus oryzae whole-cell biocatalyst that expresses Candida antarctica lipase B (r-CALB) with high esterification activity was developed. Each of soybean and palm oils was hydrolyzed using Candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-CALB was used as a catalyst. In esterification, r-CALB afforded a methyl ester content of more than 90% after 6 h with the addition of 1.5 M equivalents of methanol. Favorably, stepwise additions of methanol and a little water were unnecessary for maintaining the lipase stability of r-CALB during esterification. During long-term esterification in a rotator, r-CALB can be recycled for 20 cycles without a significant loss of lipase activity, resulting in a methyl ester content of more than 90% even after the 20th batch. Therefore, the presented reaction system using r-CALB shows promise for biodiesel production from plant oil hydrolysates. (C) 2012 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2013年05月, BIORESOURCE TECHNOLOGY, 135, 410 - 416, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Uju, Aya Nakamoto, Yasuhiro Shoda, Masahiro Goto, Wataru Tokuhara, Yoshiyuki Noritake, Satoshi Katahira, Nobuhiro Ishida, Chiaki Ogino, Noriho Kamiya

    The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80 degrees C or 100 degrees C. Short [Hpy][Cl] pretreatments, <30 min, at lower temperature accelerate subsequent enzymatic saccharification of Avicel. Over 95% conversion of pretreated Avicel to glucose was attained after 24 h enzymatic saccharification under optimal conditions, whereas regenerated Bagasse showed 1-3-fold higher conversion than untreated biomass. FT-IR analysis of both Avicel and Bagasse samples pretreated with [Hpy][Cl] or 1-ethyl-3-methyimidazolium acetate ([Emim][OAc]) revealed that these ionic liquids behaved differently during pretreatment. [Hpy][Cl] pretreatment for an extended duration (180 min) released mono- and disaccharides without using cellulase enzymes, suggesting [Hpy][Cl] has capability for direct saccharification of cellulosic feedstocks. On the basis of the results obtained, [Hpy][Cl] pretreatment enhanced initial reaction rates in enzymatic saccharification by either crystalline polymorphic alteration of cellulose or partial degradation of the crystalline cellulosic fraction in biomass. (C) 2012 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2013年05月, BIORESOURCE TECHNOLOGY, 135, 103 - 108, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Adachi, FookHee Koh, Shinji Hama, Chiaki Ogino, Akihiko Kondo

    To develop a robust whole-cell biocatalyst that works well at moderately high temperature (40-50 degrees C) with organic solvents, a thermostable lipase from Geobacillus thermocatenulatus (BTL2) was introduced into an Aspergillus oryzae whole-cell biocatalyst. The lipase-hydrolytic activity of the immobilized A. oryzae (r-BTL) was highest at 50 degrees C and was maintained even after an incubation of 24-h at 60 degrees C. In addition, r-BTL was highly tolerant to 30% (v/v) organic solvents (dimethyl carbonate, ethanol, methanol, 2-propanol or acetone). The attractive characteristics of r-BTL also worked efficiently on palm oil methanolysis, resulting in a nearly 100% conversion at elevated temperature from 40 to 50 degrees C. Moreover, r-BTL catalyzed methanolysis at a high methanol concentration without a significant loss of lipase activity. In particular, when 2 molar equivalents of methanol were added 2 times, a methyl ester content of more than 90% was achieved; the yield was higher than those of conventional whole-cell biocatalyst and commercial Candida antarctica lipase (Novozym 435). On the basis of the results regarding the excellent lipase characteristics and efficient biodiesel production, the developed whole-cell biocatalyst would be a promising biocatalyst in a broad range of applications including biodiesel production. (C) 2013 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2013年05月, ENZYME AND MICROBIAL TECHNOLOGY, 52 (6-7), 331 - 335, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Masayuki Arakawa, Chiaki Ogino, Nobuaki Shimizu

    This is the first study to demonstrate sonoelectrocatalytic disinfection using titanium dioxide (TiO2) as an anode for effective inactivation of Escherichia coli. In brief, a non-woven TiO2 fabric used as an anode and a platinum cathode were immersed in an E. coli suspension in which a positive potential was applied to TiO2 concomitant with ultrasound (US) irradiation. Two control experiments were performed using E. coli suspensions to exhibit the effects of the sonoelectrocatalytic disinfection. One was disinfection by applying a positive potential to a TiO2 electrode, but without US irradiation (electrochemical disinfection). The other was disinfection without applying a potential, but with US irradiation in the presence of TiO2 (sonocatalytic disinfection). The cell inactivation rate in sonoelectrocatalytic disinfection was synergistically much more enhanced than the combined inactivation rates in electrochemical disinfection and sonocatalytic disinfection. This synergistically enhanced inactivation rate of E. coli cells was attributable to effective reaction of the sonocatalytically generated OH radicals with E. coli cells at the surface of the TiO2 anode, which resulted from the electroadsorption of E. coli cells toward the TiO2 anode. (C) 2012 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2013年03月, ULTRASONICS SONOCHEMISTRY, 20 (2), 762 - 767, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Wakiko Mimura, Izzat Fahimuddin Mohamed Suffian, Tomokazu Amino, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    Capsid-like particles consisting of a hepatitis B core (HBc) protein have been studied for their potential as carriers for drug delivery systems (DDS). The hollow HBc particle, which is formed by the self-assembly of core proteins comprising 183 aa residues, has the ability to bind to various cells non-specifically via the action of an arginine-rich domain. In this study, we developed an engineered HBc particle that specifically recognizes and targets human epidermal growth factor receptor-related 2 (HER2)-expressing breast cancer cells. To despoil the non-specific binding property of an HBc particle, we genetically deleted the C-terminal 150-183 aa part of the core protein that encodes the arginine-rich domain (delta HBc). Then, we genetically inserted a Z(HER2) affibody molecule into the 78-81 aa position of the core protein to confer the ability of target-cell-specific recognition. The constructed Z(HER2)-displaying HBc (Z(HER2)-delta HBc) particle specifically recognized HER2-expressing SKBR3 and MCF-7 breast cancer cells. In addition, the Z(HER2)-delta HBc particle exhibited different binding amounts in accordance with the HER2 expression levels of cancer cells. These results show that the display of other types of affibody molecules on HBc particles would be an expandable strategy for targeting several kinds of cancer cells that would help enable a pinpoint DDS.

    OXFORD UNIV PRESS, 2013年03月, JOURNAL OF BIOCHEMISTRY, 153 (3), 251 - 256, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Kazuhiko Kaneda, Satoshi Kawashima, Yusuke Miyachi, Chiaki Ogino, Nobuaki Shimizu

    Single-stranded DNA aptamers recognizing human hepatocarcinoma were isolated by means of a systematic evolution of ligands by exponential enrichment using whole cells as targets (cell-SELEX). After 11 rounds of cell-SELEX procedure using human hepatoma HepG2 cells as targets and human normal hepatocyte cells as counterparts, 12 independent DNA aptamer candidate sequences were obtained. The specific interaction between selected DNA aptamers and targeted cell was confirmed. Dissociation constants of the 12 sequences obtained were also estimated in the range of 19-450 nM. Moreover, the consensus secondary structure was found in the isolated aptamers, which was responsible to the recognition of HepG2 cells. (C) 2013 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2013年03月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 23 (6), 1797 - 1802, 英語

    [査読有り]

    研究論文(学術雑誌)

  • An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules

    Yuya Nishimura, Koichi Takeda, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    2013年03月, Journal of Virological Methods, 189 (2), 393 - 396, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Takashi Yamauchi, Masafumi Kobayashi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

    The present study is the first report demonstrating that ionic liquids consisting of cholinium cations and linear carboxylate anions ([Ch][CA] ILs) can be used for pretreatment of lignocellulosic materials to enhance subsequent enzymatic saccharification. Six variants of [Ch][CA] ILs were systematically prepared by combining cholinium cations with linear monocarboxylate anions ([CnH2n+1-COO]-, n=0-2) or dicarboxylate anions ([HOOC-CnH2n+1-COO]-, n=0-2). These [Ch][CA] ILs were analyzed for their toxicity to yeast cell growth and their ability to pretreat kenaf powder for subsequent enzymatic saccharification. When assayed against yeast growth, the EC50 for choline acetate ([Ch][OAc]) was 510mM, almost one order of magnitude higher than that for 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]). The cellulose saccharification ratio after pretreatment at 110°C for 16h with [Ch][OAc] (100.6%) was almost comparable with that after pretreatment with [Emim][OAc]. Therefore, [Ch][OAc] is a biocompatible alternative to [Emim][OAc] for lignocellulosic material pretreatment. © 2012 Elsevier B.V.

    2013年02月05日, Biochemical Engineering Journal, 71, 25 - 29, 英語

    研究論文(学術雑誌)

  • イオン液体前処理バイオマスを用いたエタノール同時糖化発酵

    荻野 千秋, 山田 亮祐, 近藤 昭彦

    2013年02月, 化学工業, 64 (2), 52 - 55, 日本語

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Ryuji Yamada, Masami Matsumoto, Satoru Fukiya, Takane Katayama, Chiaki Ogino, Nobuaki Shimizu

    An image analyzing method was developed to evaluate in situ bioluminescence expression, without exposing the culture sample to the ambient oxygen atmosphere. Using this method, we investigated the effect of dissolved oxygen concentration on bioluminescence from an obligate anaerobe Bifidobacterium longum expressing bacterial luciferase which catalyzes an oxygen-requiring bioluminescent reaction. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2013年02月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115 (2), 196 - 199, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sarvesh Kumar Srivastava, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

    Room-temperature extracellular biosynthesis of gold nanoparticles (Au NPs) was achieved using Escherichia coli K12 cells without the addition of growth media, pH adjustments or inclusion of electron donors/stabilizing agents. The resulting nanoparticles were analysed by ultraviolet-visible (UV-vis) spectrophotometry, atomic force microscopy, transmission electron microscopy and X-ray diffraction. Highly dispersed gold nanoplates were achieved in the order of around 50 nm. Further, the underlying mechanism was found to be controlled by certain extracellular membrane-bound proteins, which was confirmed by Fourier transformation-infrared spectroscopy and sodium dodecyl sulfate polyacrylamide gel electrophoresis. We observed that certain membrane-bound peptides are responsible for reduction and subsequent stabilization of Au NPs (confirmed by zeta potential analysis). Upon de-activation of these proteins, no nanoparticle formation was observed. Also, we prepared a novel biocatalyst with Au NPs attached to the membrane-bound fraction of E. coli K12 cells serving as an efficient heterogeneous catalyst in complete reduction of 4-nitrophenol in the presence of NaBH4 which was studied with UV-vis spectroscopy. This is the first report on bacterial membrane-Au NP nanobiocomposite serving as an efficient heterogeneous catalyst in complete reduction of nitroaromatic pollutant in water.

    SPRINGER, 2013年02月, NANOSCALE RESEARCH LETTERS, 8 (1), 70, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Adachi, Risa Koda, Shinji Hama, Ryosuke Yamada, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

    We attempted to integrate lipase-catalyzed ethanolysis into fermentative bioethanol production. To produce bioethanol, ethanol fermentation from brown rice was conducted using a tetraploid Saccharomyces cerevisiae expressing alpha-amylase and glucoamylase. The resultant ethanol was distilled and separated into three fractions with different concentrations of water and fusel alcohols. In ethanolysis using the first fraction with 89.3% ethanol, a recombinant Aspergillus oryzae whole-cell biocatalyst expressing Fusarium heterosporum lipase (r-FHL) afforded the highest ethyl ester content of 94.0% after 96 h. Owing to a high concentration of water in the bioethanol solutions, r-FHL, which works best in the presence of water when processing ethanolysis, was found to be more suitable for the integrative process than a commercial immobilized Candida antarctica lipase. In addition, r-FHL was used for repeated-batch ethanolysis, resulting in an ethyl ester content of more than 80% even after the fifth batch. Fusel alcohols such as 1-butanol and isobutyl alcohol are thought to decrease the lipase activity of r-FHL. Using this process, a high ethyl ester content was obtained by simply mixing bioethanol, plant oil, and lipase with an appropriate adjustment of water concentration. The developed process model, therefore, would contribute to biodiesel production from only biomass-derived feedstocks. (c) 2012 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2013年02月, ENZYME AND MICROBIAL TECHNOLOGY, 52 (2), 118 - 122, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Chiaki Ogino, Masakatsu Urairi, Kazuhito Yoshida, Akihiko Kondo

    A perfluoropolymer (PFP) membrane has been prepared for use in vapor permeation to separate aqueous ethanol mixtures produced from rice straw with xylose-assimilating recombinant Saccharomyces cerevisiae. PFP membranes commonly have been used for dehydration process and possess good selectivity and high permeances. The effects of by-products during dilute acid pretreatment, addition of yeast extract, and ethanol fermentation on PFP membrane performance were investigated. While feeding mixtures of ethanol (90wt%) in water, to which individual by-products (0.1-2g/L) were added, the PFP membrane demonstrated no clear change in permeation rate (439-507gm-2h-1) or separation factor (14.9-23.5) from 2 to 4h of the process. The PFP membrane also showed no clear change in permeation rate (751-859gm-2h-1) or separation factor (12.5-13.8) while feeding the mixture (final ethanol conc.: 61wt%) of ethanol and distillation of the fermentation broth using a suspended fraction of dilute acid-pretreated rice straw for 20h. These results suggest that the PFP membrane can tolerate actual distillation liquids from ethanol fermentation broth obtained from lignocellulosic biomass pretreated with dilute acid. © 2012.

    2013年01月15日, Biochemical Engineering Journal, 70, 135 - 139, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Akiko Ohta, Sayuri Omote, Chiaki Ogino, Kenji Takahashi, Nobuaki Shimizu

    Completely bio-derived cholinium ionic liquids (ILs) such as choline acetate (ChOAc) are reported to be less expensive, more biocompatible, and more biodegradable and bio-renewable in comparison with the imidazolium ILs that are conventionally used for the pretreatment of lignocellulosic biomass. We demonstrated here, for the first time, that the cholinium-IL-assisted pretreatment of lignocellulosic biomass is enhanced by ultrasound irradiation in comparison with conventional heating. The cellulose saccharification ratio of bamboo powder was approximately 55% when pretreated thermally in ChOAc at 110°C for 60. min. Conversely, after ultrasonic pretreatment in the same IL at 25°C for 60. min, 92% of cellulose was hydrolyzed to glucose. Moreover, X-ray diffractometry and Fourier-transform infrared spectrometry analyses revealed that the cellulose crystallinity of pretreated bamboo powder was lower in case of ultrasonic pretreatment in ChOAc than in case of thermal pretreatment in the same IL. © 2012 Elsevier B.V.

    2013年01月05日, Chemical Engineering Journal, 215-216, 811 - 818, 英語

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Hiroshi Soda, Chiaki Ogino, Kenji Takahashi, Nobuaki Shimizu

    The pretreatment efficiency of weight ratios ranging from 0 to 10 of the ionic liquid, cholinum IL, to bamboo powder was investigated. An IL/biomass ratio of 3 g/g was critical to obtain a cellulose saccharification ratio of 80%. At this ratio, the treated bamboo powder remained as a solid. The solid-state pretreatment required a minimum amount of cholinium IL, which could reduce the cost of IL-assisted pretreatment and reduce the amount of wastewater generated in the process. (C) 2012 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2013年01月, BIORESOURCE TECHNOLOGY, 128, 188 - 192, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sarvesh Kumar Srivastava, Takashi Hasegawa, Ryosuke Yamada, Chiaki Ogino, Minoru Mizuhata, Akihiko Kondo

    A facile, eco-friendly, room-temperature method for rapid one-pot synthesis of Au@Pd bimetallic nanoparticles exhibiting a core-shell morphology (similar to 60 nm) has been developed based on the successive reduction of Au(III) and Pd(II) precursors with tryptophan (Trp) in an aqueous environment. The unique supramolecular chemistry arising due to the hydrogen bonded indole group layer over the Au core seemed critical in the formation Pd shell. The core-shell morphology and surface analysis of the resulting Au@Pd nanoparticles were confirmed by aberration corrected scanning transmission electron microscopy followed by X-ray photoelectron spectroscopy. The formation of the core (Au) and shell (Pd) was also confirmed by Energy Dispersive X-Ray elemental scanning analysis. The resulting Au, Pd and Au@Pd nanoparticles were also analysed by UV-Vis spectroscopy, X-ray diffraction and Dynamic Light Scattering. Our results suggest a simple coordination mechanism where the pre-stabilized poly-Trp Au core serves as a template to facilitate the subsequent reduction of Pd(II) via active carboxyl groups. This study effectively demonstrates for the first time that core-shell nanoparticle synthesis (reduction and stabilization) can be effectively achieved by simple amino acids like Trp in an aqueous reaction mixture.

    ROYAL SOC CHEMISTRY, 2013年, RSC ADVANCES, 3 (40), 18367 - 18372, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryosuke Yamada, Yuki Nakatani, Chiaki Ogino, Akihiko Kondo

    Efficient degradation of cellulosic biomass requires the synergistic action of the cellulolytic enzymes endoglucanase, cellobiohydrolase, and β-glucosidase. Although there are many reports describing consolidation of hydrolysis and fermentation steps using recombinant Saccharomyces cerevisiae that express cellulolytic enzymes, the efficiency of cellulose degradation has not been sufficiently improved. Although the yeast S. cerevisiae cannot take up cellooligosaccharide, some fungi can take up and assimilate cellooligosaccharide through a cellodextrin transporter. In this study, a S. cerevisiae strain co-expressing genes for several cell surface display cellulases and the cellodextrin transporter was constructed for the purpose of improving the efficiency of direct ethanol fermentation from phosphoric acid swollen cellulose (PASC). The cellulase/cellodextrin transporter-coexpressing strain produced 1.7-fold more ethanol (4.3 g/L) from PASC during a 72-h fermentation than did a strain expressing cellulase only (2.5 g/L). Direct ethanol production from PASC by the recombinant S. cerevisiae strain was improved by co-expression of cellulase display and cellodextrin transporter genes. These results suggest that cellulase- and cellodextrin transporter-co-expressing S. cerevisiae could be a promising technology for efficient direct ethanol production from cellulose. © 2013 Bel-Rhlid et al.

    Springer Verlag, 2013年, AMB Express, 3 (1), 1 - 7, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Jun Ishii, Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo

    A bio-nanocapsule (BNC), a hollow particle composed of hepatitis B virus (HBV) surface antigen (HBsAg), and liposome (LP) conjugation method (BNC/LP) has been recently developed by Jung et al. (2008). The BNC/LP complex carrier could successfully deliver fluorescence-labeled beads (100 nm) into liver cells. In this study, we report the promising delivery of proteins incorporated in the complex carriers, which were prepared by the BNC/LP conjugation method with specificity-altered BNC and composition-varied LPs. The specificity-altered BNC, Z(HER2)-BNC was developed by replacing the hepatocyte recognition site of BNC with Z(HER2) binding to HER2 receptor specifically. Using green fluorescent protein (GFP; 27 kDa) and cellular cytotoxic protein (exotoxin A; 66 kDa) for the delivery, we herein present the impact of different charges attributed to the composition of the LP on specific cell targeting and cellular uptake of the complex carriers. In addition, we demonstrate that the mixture prepared by mixing LPs with helper lipid possessing endosomal escaping ability boosts the functional expression of the cellular cytotoxic exotoxin A activity specifically. Finally, we further show the blending ratio of the LP mixture and Z(HER2)-BNC is a critical factor in determining the highly-efficient expression of the cytotoxic activity of exotoxin A.

    INFORMA HEALTHCARE, 2012年12月, JOURNAL OF DRUG TARGETING, 20 (10), 897 - 905, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Fumiyoshi Okazaki, Jun-ichi Aoki, Soichiro Tabuchi, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    We have constructed a filamentous fungus Aspergillus oryzae that secretes a llama variable heavy-chain antibody fragment (V-HH) that binds specifically to epidermal growth factor receptor (EGFR) in a culture medium. A major improvement in yield was achieved by fusing the V-HH with a Taka-amylase A signal sequence (sTAA) and a segment of 28 amino acids from the N-terminal region of Rhizopus oryzae lipase (N28). The yields of secreted, immunologically active anti-EGFR V-HH reached 73.8 mg/1 in a Sakaguchi flask. The V-HH fragments were released from the sTAA or N28 proteins by an indigenous A. oryzae protease during cultivation. The purified recombinant V-HH fragment was specifically recognized and could bind to the EGFR with a high affinity.

    SPRINGER, 2012年10月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 96 (1), 81 - 88, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takanori Tanino, Tomonori Ito, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

    Four kinds of transporters. HXT1 and HXY7 from Saccharomyces cerevisiae, and GXF1 and GXS1 from Candida intermedia, were overexpressed in xylose-metabolizing S. cerevisiae harboring a xyloseisomerase-based pathway. Overexpression of transporter enhanced sugar consumption and ethanol production, and GXF1 was efficient for ethanol fermentation from both glucose and xylose. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114 (2), 209 - 211, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tsutomu Tanaka, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

    Yeasts are promising hosts for industrial bio-refinery applications. In yeast cell surface displays, functional proteins, such as cellulases or lipases, are genetically fused to an anchor protein and expressed on the cell surface. Saccharomyces cerevisiae is the most commonly used yeast for cell surface display. Engineered yeasts have been utilized for a variety of applications, such as bioethanol production, chemicals synthesis, adsorption of environmental pollutants, and protein evolution. Here, we summarize recent developments in yeast cell surface display techniques for bio-refinery applications, including methods using hosts such as Pichia pastoris, Yarrowia lipolytica, and S. cerevisiae, focusing on the characteristics of anchor proteins and applications.

    SPRINGER, 2012年08月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 95 (3), 577 - 591, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chihiro Takahashi, Junki Shirakawa, Takeyuki Tsuchidate, Naoko Okai, Kazuki Hatada, Hideki Nakayama, Toshihiro Tateno, Chiaki Ogino, Akihiko Kondo

    Gamma-amino butyric acid (GABA) is a component of pharmaceuticals, functional foods, and the biodegradable plastic polyamide 4. Here, we report a simple and robust system to produce GABA from glucose using the recombinant Corynebacterium glutamicum strain GAD, which expresses GadB, a glutamate decarboxylase encoded by the gadB gene of Escherichia coli W3110. As confirmed by HPLC analysis. GABA fermentation by C. glutamicum GAD cultured at 30 degrees C in GABA Production 1 (GP1) medium containing 50 g/L glucose without the addition of glutamate yielded 8.07 +/- 1.53 g/L extracellular GABA after 96h. Addition of 0.1 mM pyridoxal 5'-phosphate (PLP) was found to enhance the production of GABA, whereas Tween 40 was unnecessary for GABA fermentation. Using the optimized GABA Production 2 (GP2) medium, which contained 50 g/L glucose and 0.1 mM PLP, fermentation was performed in a flask at 30 degrees C with 10% (v/v) seed culture of C. glutamicum GAD. GABA was produced in the culture supernatant with a yield of 12.37 +/- 0.88 g/L after 72 h with a space-time yield of 0.172 g/L/h, which is the highest yield obtained to date for GABA from fermentation with glucose as a main carbon source. (C) 2012 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2012年08月, ENZYME AND MICROBIAL TECHNOLOGY, 51 (3), 171 - 176, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuki Soma, Kentaro Inokuma, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Masahiro Okamoto, Taizo Hanai

    Efficient bio-production from lignocellulosic biomass is required for the purpose of developing an inexpensive, practical bio-refinery process. As one approach to address this problem, we genetically engineered Escherichia coli to produce isopropanol directly from cellobiose via the cellobiose degradation by Beta-Glucosidase (BGL) on the cell surface. First, we investigated the cellobiose consumption of two E. coli strains with the BGL protein from Thermobifida filsca YX (Tfu0937) fused to the anchor protein Blc (Tfu0937/Blc) using different fusion sites. Next, we introduced the synthetic pathway for isopropanol production into those strains and compared their isopropanol production in the presence of glucose. Based on the results of these assays, TA212/pTA411, which was introduced Tfu-Blc fused protein expression system and the synthetic pathway for isopropanol production, was selected for the directly isopropanol production from cellobiose. TA212/pTA411 produced 69.0 +/- 11.6 mM isopropanol at 21 h of fermentation, whereas TA212/pTA147, which did not introduced the BGL/anchor fused protein but was introduced the synthetic pathway for isopropanol production, showed no cellobiose consumption and no isopropanol production during fermentation. To our knowledge, this is the first report of the production of a bio-product from cellobiose using E. coli. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年07月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114 (1), 80 - 85, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMAKAWA S, YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    A diploid yeast strain displaying both α-amylase and glucoamylase was developed for repeated fermentation from raw starch. First, the construct of α-amylase was optimized for cell surface display, as there have been no reports of α-amylase-displaying yeast. The modified yeast displaying both glucoamylase and α-amylase produced 46.5. g/l of ethanol from 200. g/l of raw corn starch after 120. h of fermentation, and this was 1.5-fold higher when compared to native α-amylase-displaying yeast. Using the glucoamylase and modified α-amylase co-displaying diploid strain, we repeated fermentation from 100. g/l of raw starch for 23 cycles without the loss of α-amylase or glucoamylase activity. The average ethanol productivity and yield during repeated fermentation were 1.61. g/l/h and 76.6% of the theoretical yield, respectively. This novel yeast may be useful for reducing the cost of bio-ethanol production and may be suitable for industrial-scale bio-ethanol production. © 2012 Elsevier Inc.

    2012年05月10日, Enzyme and Microbial Technology, 50 (6-7), 343 - 347, 英語

    [査読有り]

    研究論文(学術雑誌)

  • イオン液体と超音波照射を組み合わせたリグノセルロース前処理によるバイオマス・リファイナリー

    高橋 憲司, 仁宮 一章, 荻野 千秋, 清水 宣明

    2012年05月, バイオインダストリー, 29, 38 - 45, 日本語

    研究論文(学術雑誌)

  • Kazuaki Ninomiya, Chiaki Ogino, Shuhei Oshima, Shiro Sonoke, Shun-ichi Kuroda, Nobuaki Shimizu

    Our previous study suggested new sonodynamic therapy for cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with a protein specifically recognizing target cells and subsequent generation of hydroxyl radicals from TiO2 NPs activated by external ultrasound irradiation (called TiO2/US treatment). The present study first examined the uptake behavior of TiO2 NPs modified with pre-S1/S2 (model protein-recognizing hepatocytes) by HepG2 cells for 24 h. It took 6 h for sufficient uptake of the TiO2 NPs by the cells. Next, the effect of the TiO2/US treatment on HepG2 cell growth was examined for 96 h after the 1 MHz ultrasound was irradiated (0.1 W/cm(2), 30 s) to the cells which incorporated the TiO2 NPs. Apoptosis was observed at 6 h after the TiO2/US treatment. Although no apparent cell-injury was observed until 24 h after the treatment, the viable cell concentration had deteriorated to 46% of the control at 96 h. Finally, the TiO2/US treatment was applied to a mouse xenograft model. The pre-S1/S2-immobilized TiO2 (0.1 mg) was directly injected into tumors, followed by 1 MHz ultrasound irradiation at 1.0 W/cm(2) for 60s. As a result of the treatment repeated five times within 13 days, tumor growth could be hampered up to 28 days compared with the control conditions. (C) 2011 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2012年05月, ULTRASONICS SONOCHEMISTRY, 19 (3), 607 - 614, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashi Kondo, Hironori Tezuka, Jun Ishii, Fumio Matsuda, Chiaki Ogino, Akihiko Kondo

    The production of higher alcohols by engineered bacteria has received significant attention. The budding yeast, Saccharomyces cerevisiae, has considerable potential as a producer of higher alcohols because of its capacity to naturally fabricate fusel alcohols, in addition to its robustness and tolerance to low pH. However, because its natural productivity is not significant, we considered a strategy of genetic engineering to increase production of the branched-chain higher alcohol isobutanol, which is involved in valine biosynthesis. Initially, we overexpressed 2-keto acid decarboxylase (KDC) and alcohol dehydrogenase (ADH) in S. cerevisiae to enhance the endogenous activity of the Ehrlich pathway. We then overexpressed Ilv2, which catalyzes the first step in the valine synthetic pathway, and deleted the PDC1 gene encoding a major pyruvate decarboxylase with the intent of altering the abundant ethanol flux via pyruvate. Through these engineering steps, along with modification of culture conditions, the isobutanol titer of S. cerevisiae was elevated 13-fold, from 11 mg/l to 143 mg/l, and the yield was 6.6 mg/g glucose, which is higher than any previously reported value for S. cerevisiae. (C) 2012 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2012年05月, JOURNAL OF BIOTECHNOLOGY, 159 (1-2), 32 - 37, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jun Ishii, Nobuo Yoshimoto, Kenji Tatematsu, Shun'ichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    G-protein-coupled receptors (GPCRs) regulate a wide variety of physiological processes and are important pharmaceutical targets for drug discovery. Here, we describe a unique concept based on yeast cell-surface display technology to selectively track eligible peptides with agonistic activity for human GPCRs (Cell Wall Trapping of Autocrine Peptides (CWTrAP) strategy). In our strategy, individual recombinant yeast cells are able to report autocrine-positive activity for human GPCRs by expressing a candidate peptide fused to an anchoring motif. Following expression and activation, yeast cells trap autocrine peptides onto their cell walls. Because captured peptides are incapable of diffusion, they have no impact on surrounding yeast cells that express the target human GPCR and non-signaling peptides. Therefore, individual yeast cells can assemble the autonomous signaling complex and allow single-cell screening of a yeast population. Our strategy may be applied to identify eligible peptides with agonistic activity for target human GPCRs.

    PUBLIC LIBRARY SCIENCE, 2012年05月, PLOS ONE, 7 (5), e37136, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kiyotaka Y. Hara, Songhee Kim, Kentaro Kiriyama, Hideyo Yoshida, Shogo Arai, Jun Ishii, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Glutathione is a valuable tripeptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is industrially produced by fermentation using Saccharomyces cerevisiae. Before the glutathione fermentation process with S. cerevisiae, a glucose extraction process from starchy materials is required. This glucose extraction is usually carried out by converting starchy materials to starch using high-temperature cooking and subsequent hydrolysis by amylases to convert starch to glucose. In this study, to develop an energy-saving glutathione production process by reducing energy consumption during the cooking step, we efficiently produced glutathione from low-temperature cooked rice using amylase-expressing S. cerevisiae. The combination of the amylase-expressing yeast with low-temperature cooking is potentially applicable to a variety of energy-saving bio-production methods of chemicals from starchy bio-resources.

    WILEY-BLACKWELL, 2012年05月, BIOTECHNOLOGY JOURNAL, 7 (5), 686 - 689, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Noda, Eiichi Kitazono, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    Background: Benzoic acid is one of the most useful aromatic compounds. Despite its versatility and simple structure, benzoic acid production using microbes has not been reported previously. Streptomyces are aerobic, Gram-positive, mycelia-forming soil bacteria, and are known to produce various kinds of antibiotics composed of many aromatic residues. S. maritimus possess a complex amino acid modification pathway and can serve as a new platform microbe to produce aromatic building-block compounds. In this study, we carried out benzoate fermentation using S. maritimus. In order to enhance benzoate productivity using cellulose as the carbon source, we constructed endo-glucanase secreting S. maritimus. Results: After 4 days of cultivation using glucose, cellobiose, or starch as a carbon source, the maximal level of benzoate reached 257, 337, and 460 mg/l, respectively. S. maritimus expressed beta-glucosidase and high amylase-retaining activity compared to those of S. lividans and S. coelicolor. In addition, for effective benzoate production from cellulosic materials, we constructed endo-glucanase-secreting S. maritimus. This transformant efficiently degraded the phosphoric acid swollen cellulose (PASC) and then produced 125 mg/l benzoate. Conclusions: Wild-type S. maritimus produce benzoate via a plant-like beta-oxidation pathway and can assimilate various carbon sources for benzoate production. In order to encourage cellulose degradation and improve benzoate productivity from cellulose, we constructed endo-glucanase-secreting S. maritimus. Using this transformant, we also demonstrated the direct fermentation of benzoate from cellulose. To achieve further benzoate productivity, the L-phenylalanine availability needs to be improved in future.

    BIOMED CENTRAL LTD, 2012年04月, MICROBIAL CELL FACTORIES, 11 (1), 49, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 酸化チタン粒子のバイオ応用

    荻野 千秋

    2012年03月, 生物工学会誌, 90, 110 - 114, 日本語

    研究論文(学術雑誌)

  • Daisuke Sugimori, Yusaku Matsumoto, Yu Tomita, Kazutaka Murayama, Chiaki Ogino

    Sphingomyelinase C (SMC) of the actinomycete, Streptomyces griseocarneus NBRC13471, was constitutively expressed to high levels using Streptomyces lividans host and thereafter was extracellularly secreted into the cell culture. Purified SMC had a high specific activity (approximately 550-950 U/mg) and was obtained in high yields (approximately 120 mg/L of culture). SMC activity was enhanced by MgCl2, and the maximum activity (542 +/- 25 U/mg) was observed in the presence of 1.5 mol/L (M) MgCl2. Dynamic light scattering analysis proved that the highest specific SMC activity was obtained with the smallest mixed micelles of sphingomyelin (SM) and Triton X-100. The turnover rate (k(cat)), K-m and k(cat)/K-m values for SM were 346 s(-1), 0.458 mM, and 756 mM(-1) s(-1), respectively, in the presence of 1 M MgCl2. The kcat was strongly influenced by the MgCl2 concentration. By contrast, the K-m value was independent of the MgCl2 concentration and was almost constant. Circular dichroism spectroscopy indicated that MgCl2 did not cause local structural changes in SMC. From these results, we concluded that the SMC activity enhancement by MgCl2 was caused by the increased specific surface area of the mixed micelles composed of substrate, SM, and Triton X-100. (C) 2011 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012年02月, PROTEIN EXPRESSION AND PURIFICATION, 81 (2), 151 - 156, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Glutathione is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae, and supplementation of fermentation with several amino acids can increase intracellular GSH content. More recently, however, focus has been given to protein as a resource for biofuel and fine chemical production. We demonstrate that expression of a protease on the cell surface of S. cerevisiae enables the direct use of keratin and soy protein as a source of amino acids and that these substrates enhanced intracellular GSH content. Furthermore, fermentation using soy protein also enhanced cell concentration. GSH fermentation from keratin and to a greater extent from soy protein using protease-displaying yeast yielded greater GSH productivity compared to GSH fermentation with amino acid supplementation. This protease-displaying yeast is potentially applicable to a variety of processes for the bio-production of value-added chemicals from proteinaceous biomass resources.

    SPRINGER, 2012年02月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 93 (4), 1495 - 1502, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Uju, Yasuhiro Shoda, Aya Nakamoto, Masahiro Goto, Wataru Tokuhara, Yoshiyuki Noritake, Satoshi Katahira, Nobuhiro Ishida, Kazunori Nakashima, Chiaki Ogino, Noriho Kamiya

    The potential of 1-buthyl-3-methylpyridinium chloride, [Bmpy][Cl], as a pretreatment solvent for lignocellulosic biomasses, Bagasse and Eucalyptus, was investigated. The yields of regenerated biomasses ranged between 35% and 96%, and varied according to the pretreatment time, type of ionic liquid (IL) and biomass. The pretreatment of the biomass with [Bmpy][Cl] resulted in up to 8-fold increase in the cellulose conversion when compared with the untreated biomass. For a short pretreatment period (i.e., 10 min), [Bmpy][Cl] showed better performance than 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) with respect to the initial enzymatic saccharification rates. The increase in the reaction rates with [Emim][OAc] treatment was because of a reduction in the cellulose crystallinity. In contrast, a decrease in the crystallinity index was not clearly observed for the biomass pretreated with [Bmpy][Cl], and the enhancement of the enzymatic saccharification rates using this IL is presumably due to a reduction in the degree of polymerization of cellulose in the biomass. (C) 2011 Published by Elsevier Ltd.

    ELSEVIER SCI LTD, 2012年01月, BIORESOURCE TECHNOLOGY, 103 (1), 446 - 452, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yuya Nishimura, Takuya Shishido, Jun Ishii, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    Bio-nanocapsules (BNCs) are hollow nanoparticles composed of the L protein of hepatitis B virus (HBV) surface antigen (HBsAg), which can specifically introduce genes and drugs into various kinds of target cells. Although the classic electroporation method has typically been used to introduce highly charged molecules such as DNA, it is rarely adopted for proteins due to its very low efficiency. In this study, a novel approach to the preparation of BNC was established whereby a target protein was pre-encapsulated during the course of nanoparticle formation. Briefly, because of the process of BNC formation in a budding manner on the endoplasmic reticulum (ER) membrane, the association of target proteins to the ER membrane with lipidation sequences (ER membrane localization sequences) could directly generate protein-encapsulating BNC in collaboration with co-expression of the L proteins. Since the membrane-localized proteins are automatically enveloped into BNCs during the budding event, this method can be protect the proteins and BNCs from damage caused by electroporation and obviate the need for laborious consideration to study the optimal conditions for protein encapsulation. This approach would be a useful method for encapsulating therapeutic candidate proteins into BNCs. (C) 2011 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2012年01月, JOURNAL OF BIOTECHNOLOGY, 157 (1), 124 - 129, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    Transglutaminase from Streptoverticillium cinnamoneum (StvcMTG) was produced using recombinant Streptomyces lividans. When grown on glycerol and xylose as sole carbon sources, S. lividans/StvcMTG produced 360 and 530 mg of StvcMTG per liter, respectively. With starch and xylan, the strain produced 230 and 400 mg of StvcMTG per liter, respectively. Recombinant S. lividans/encP, which expresses phenylalanine ammonia lyase from Streptomyces maritimus, produced 160 mg/L of cinnamic acid from cellulose. These results show that S. lividans can assimilate various carbon sources and produce useful compounds in desirable quantities. (C) 2011 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2012年01月, BIORESOURCE TECHNOLOGY, 104, 648 - 651, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yusuke Miyachi, Chiaki Ogino, Tomokazu Amino, Akihiko Kondo

    Atomic force microscopy (AFM) can dynamically detect the adhesion or affinity force between a sample surface and a cantilever. This feature is useful as a detection method using aptamers - single-strand DNA that recognizes its target with very high affinity. The present study proposes a novel DNA aptamer-based sensing system using AFM. In this study, thrombin was chosen as the target molecule, and a DNA aptamer-based AFM sensing system based on competition was developed. The affinity force between the gold chip and the cantilever decreased as the concentration of thrombin increased. Moreover, a low detection limit of 0.2 nM was achieved. Therefore, the AFM sensing system used would be appropriate for the measurement of various chemical compounds. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2011年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112 (5), 511 - 514, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    In order to achieve efficient d-lactic acid fermentation from a mixture of xylose and glucose, the xylose-assimilating xylAB operon from Lactobacillus pentosus (PXylAB) was introduced into an l-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum (Delta ldhL1-xpk1::tkt-Delta xpk2) strain in which the phosphoketolase 1 gene (xpk1) was replaced with the transketolase gene (tkt) from Lactococcus lactis, and the phosphoketolase 2 (xpk2) gene was deleted. Two copies of xylAB introduced into the genome significantly improved the xylose fermentation ability, raising it to the same level as that of Delta ldhL1-xpk1::tkt-Delta xpk2 harboring a xylAB operon-expressing plasmid. Using the two-copy xylAB integrated strain, successful homo-d-lactic acid production was achieved from a mixture of 25 g/l xylose and 75 g/l glucose without carbon catabolite repression. After 36-h cultivation, 74.2 g/l of lactic acid was produced with a high yield (0.78 g per gram of consumed sugar) and an optical purity of d-lactic acid of 99.5%. Finally, we successfully demonstrated homo-d-lactic acid fermentation from a mixture of three kinds of sugar: glucose, xylose, and arabinose. This is the first report that describes homo-d-lactic acid fermentation from mixed sugars without carbon catabolite repression using the xylose-assimilating pathway integrated into lactic acid bacteria.

    SPRINGER, 2011年10月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92 (1), 67 - 76, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoru Shinkawa, Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    In order to achieve efficient homo L-lactic acid fermentation from xylose, we first carried out addition of xylose assimilation ability to Lactococcus lactis IL 1403 by introducing a plasmid carrying the xylRAB genes from L. lactis IO-1 (pXylRAB). Then modification of xylose assimilation pathway was carried out. L. lactis has two pathways for xylose assimilation called the phosphoketolase pathway (PK pathway) that produces both lactic acid and acetic acid and the pentose phosphate pathway (PP pathway) that produces only lactic acid as a final product. Thus a mutant strain that disrupted its phosphokeolase gene (ptk) was constructed. The Delta ptk mutant harboring pXylRAB lacked the PK pathway and produced predominantly lactic acid from xylose via the PP pathway, although its fermentation rate slightly decreased. Further introduction of the transketolase gene (tkt) to disrupted ptk locus led restoration of fermentation rate and this was attributed to enhancement of the PP pathway. As a result, ptk::tkt strain harboring pXylRAB produced 50.1 g/l of L-lactic acid from xylose with a high optical purity of 99.6% and a high yield of 1.58 (moles per mole xylose consumed) that is close to theoretical value of 1.67 from xylose.

    SPRINGER, 2011年09月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91 (6), 1537 - 1544, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tsutomu Tanaka, Hitomi Kawabata, Chiaki Ogino, Akihiko Kondo

    We demonstrated direct assimilation of cellooligosaccharide using Escherichia coli displaying beta-glucosidase (BGL). BGL from Thermobifida fusca YX (Tfu0937) was displayed on the E. coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h.

    AMER SOC MICROBIOLOGY, 2011年09月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77 (17), 6265 - 6270, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Fumio Matsuda, Masanori Yamasaki, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    Biomass properties of rice straws were compared among eight cultivars that formed a mini diverse set. The ethanol productivity from rice straws was evaluated employing a laboratory-scale method based on dilute acid-hydrolysis pretreatment. The results indicated significant variation in biomass properties among the cultivars.

    TAYLOR & FRANCIS LTD, 2011年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75 (8), 1603 - 1605, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyo Yoshida, Kiyotaka Y. Hara, Kentaro Kiriyama, Hideki Nakayama, Fumiyoshi Okazaki, Fumio Matsuda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    We developed a novel enzymatic glutathione (GSH) production system using Saccharomyces cerevisiae as a whole-cell biocatalyst, and improved its GSH productivity by metabolic engineering. We demonstrated that the metabolic engineering of GSH pathway and ATP regeneration can significantly improve GSH productivity by up to 1.7-fold higher compared with the parental strain, respectively. Furthermore, the combination of both improvements in GSH pathway and ATP regeneration is more effective (2.6-fold) than either improvement individually for GSH enzymatic production using yeast. The improved whole-cell biocatalyst indicates its great potential for applications to other kinds of ATP-dependent bioproduction.

    SPRINGER, 2011年08月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91 (4), 1001 - 1006, 英語

    [査読有り]

    研究論文(学術雑誌)

  • OKAZAKI Fumiyoshi, OGINO Chiaki, KONDO Akihiko, MIKAMI B, KUREBAYASHI Yoichi, TSURUTA Hiroki

    Crystals of beta-1,3-xylanase (1,3-beta-D-xylan xylanohydrolase; EC 3.2.1.32) from Thermotoga neapolitana strain DSM 4359 with maximum dimensions of 0.2 x 0.1 x 0.02 mm were grown using the sitting-drop vapour-diffusion method at 293 K over 24 h. The crystals diffracted to a resolution of 1.82 angstrom, allowing structure determination. The crystals belonged to space group P2(1), with unit-cell parameters a = 39.061, b = 75.828, c = 52.140 angstrom; each asymmetric unit cell contained a single molecule.

    WILEY-BLACKWELL, 2011年07月, Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 67, 779 - 781, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Adachi, Shinji Hama, Takao Numata, Kazunori Nakashima, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    An Aspergillus oryzae whole-cell biocatalyst which coexpresses Fusarium heterosporum lipase (FHL) and mono- and di-acylglycerol lipase B (mdlB) in the same cell has been developed to improve biodiesel production. By screening a number of transformants, the best strain was obtained when FHL gene was integrated into A. oryzae chromosome using (S)C selection marker while mdlB was integrated using niaD selection marker. The reaction system using the lipase-coexpressing whole-cells was found to be superior in biodiesel production to others such as lipase-mixing and two-step reactions, affording the highest reaction rate and the highest ME content (98%). Moreover, an ME content of more than 90% was maintained during 10 repeated batch cycles. The whole-cell biocatalyst developed in this work would be promising biocatalysts for efficient biodiesel production. (C) 2011 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, 2011年06月, BIORESOURCE TECHNOLOGY, 102 (12), 6723 - 6729, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomohiro Takaya, Risa Koda, Daisuke Adachi, Kazunori Nakashima, Junpei Wada, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

    In the present study, a system with high lipase expression in Aspergillus oryzae was developed using an improved enolase promoter (P-enoA124) and the 5' , methanolysiuntranslated region of a heat-shock protein (Hsp-UTR). P-enoA142 enhanced the transcriptional level of a heterologous lipase gene and Hsp-UTR improved its translational efficiency. Fusarium heterosporum lipase (FHL) was inserted into a pSENSU-FHL expression vector harboring P-enoA142 and Hsp-UTR and was transformed into an A. oryzae NS4 strain. Transformants possessing pSENSU-FHL in single (pSENSU-FHL#1) and double copies (pSENSU-FHL#2) were selected to evaluate the lipase activity of the whole-cell biocatalyst. The two strains, pSENSU-FHL#1 and #2, showed excellent lipase activity in hydrolysis compared with the strain transformed with conventional expression vector pNAN8142-FHL. Furthermore, by using pSENSU-FHL#2s could proceed much more effectively without deactivation, which allowed a swift addition of methanol to the reaction mixture, thereby reducing reaction time.

    SPRINGER, 2011年05月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90 (3), 1171 - 1177, 英語

    [査読有り]

    研究論文(学術雑誌)

  • TSUCHIDATE Takeyuki, TATENO Toshihiro, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    We demonstrate glutamate production from beta-glucan using endoglucanase (EG)-expressing Corynebacterium glutamicum. The signal sequence torA derived from Escherichia coli K12, which belongs to the Tat pathway, was suitable for secreting EG of Clostridium thermocellum using C. glutamicum as a host. Using the torA signal sequence, endoglucanase from Clostridium cellulovorans 743B was successfully expressed, and the secreted EG produced 123 mg of reducing sugar from 5 g of beta-glucan at 30 A degrees C for 72 h, which is the optimal condition for C. glutamicum growth. Subsequently, glutamate fermentation from beta-glucan was carried out with the addition of Aspergillus aculeatus beta-glucosidase produced by recombinant Aspergillus oryzae. Using EG-secreting C. glutamicum, 178 mg/l of glutamate was produced from 15 g of beta-glucan. This is the first report of glutamate fermentation from beta-glucan using endoglucanase-secreting C. glutamicum.

    SPRINGER, 2011年05月, Applied Microbiology and Biotechnology, 90 (3), 895 - 901, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Noda, Takaya Miyazaki, Takanori Miyoshi, Michiru Miyake, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    Cinnamic acid production was demonstrated using Streptomyces as a host. A gene encoding phenylalanine ammonia lyase (PAL) from Streptomyces maritimus was introduced into Streptomyces lividans, and its expression was confirmed by Western blot analysis. After 4 days cultivation using glucose as carbon source, the maximal level of cinnamic acid reached 210 mg/L. When glycerol (30 g/L) was used as carbon source, the maximal level of produced cinnamic acid reached 450 mg/L. In addition, using raw starch, xylose or xylan as carbon source, the maximal level of cinnamic acid reached 460, 300, and 130 mg/L, respectively. We demonstrated that S. lividans has great potential to produce cinnamic acid as well as other aromatic compounds.

    SPRINGER HEIDELBERG, 2011年05月, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 38 (5), 643 - 648, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryosuke Yamada, Syun-ichi Yamakawa, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Efficient ethanol producing yeast Saccharomyces cerevisiae cannot produce ethanol from raw starch directly. Thus the conventional ethanol production required expensive and complex process. In this study, we developed a direct and efficient ethanol production process from high-yielding rice harvested in Japan by using amylase expressing yeast without any pretreatment or addition of enzymes or nutrients. Ethanol productivity from high-yielding brown rice (1.1 g/L/h) was about 5-fold higher than that obtained from purified raw corn starch (0.2 g/L/h) when nutrients were added. Using an inoculum volume equivalent to 10% of the fermentation volume without any nutrient supplementation resulted in ethanol productivity and yield reaching 1.2 g/L/h and 101%, respectively, in a 24-h period. High-yielding rice was demonstrated to be a suitable feedstock for bioethanol production. In addition, our polyploid amylase-expressing yeast was sufficiently robust to produce ethanol efficiently from real biomass. This is first report of direct ethanol production on real biomass using an amylase-expressing yeast strain without any pretreatment or commercial enzyme addition. (C) 2011 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2011年04月, ENZYME AND MICROBIAL TECHNOLOGY, 48 (4-5), 393 - 396, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Extracellular production of a sphingomyelinase fromStreptomyces griseocarneus using Streptomyces lividans

    SUGIMORI, D, TOMITA, Y, MATSUMOTO, Y, OGINO Chiaki

    2011年03月, Biotechnology Letters, 33(4), 727-731, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyo Yoshida, Shogo Arai, Kiyotaka Y. Hara, Ryosuke Yamada, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Glutathione is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae. We demonstrated that expression of amylase genes in glutathione-producing S. cerevisiae enables direct use of starch as a carbon source, thus eliminating the Crabtree effect that is caused by excess glucose. Consequently, cell growth and glutathione productivity were significantly improved. This approach is potentially applicable to a variety of fermentative processes for production of value-added chemicals under aerobic conditions.

    SPRINGER, 2011年03月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89 (5), 1417 - 1422, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiaki Ogino, Shuhei Noda, Akihiko Kondo, Naoko Okai, Tsutomu Tanaka

    Cinnamic acid production was demonstrated using Streptomyces as a host. A gene encoding phenylalanine ammonia lyase (PAL) from Streptomyces maritimus was introduced into Streptomyces lividans and its expression was confirmed by western blot analysis. After 4 days cultivation using glucose as a carbon source, the maximal level of cinnamic acid reached 210 mg/L. When glycerol (30 g/L) was used as the carbon source, the maximal level of produced cinnamic acid reached 450 mg/L. In addition, using raw starch, xylose and xylan as the carbon source, the maximal level of cinnamic acid reached 460, 300, 130 mg/L, respectively. We demonstrated that S. lividans has great potential to produce cinnamic acid as well as other aromatic compounds. © 2011 IEEE.

    2011年, 2011 Defense Science Research Conference and Expo, DSR 2011, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Background: Hydrolysis of cellulose requires the action of the cellulolytic enzymes endoglucanase, cellobiohydrolase and b-glucosidase. The expression ratios and synergetic effects of these enzymes significantly influence the extent and specific rate of cellulose degradation. In this study, using our previously developed method to optimize cellulase-expression levels in yeast, we constructed a diploid Saccharomyces cerevisiae strain optimized for expression of cellulolytic enzymes, and attempted to improve the cellulose-degradation activity and enable direct ethanol production from rice straw, one of the most abundant sources of lignocellulosic biomass. Results: The engineered diploid strain, which contained multiple copies of three cellulase genes integrated into its genome, was precultured in molasses medium (381.4 mU/g wet cell), and displayed approximately six-fold higher phosphoric acid swollen cellulose (PASC) degradation activity than the parent haploid strain (63.5 mU/g wet cell). When used to ferment PASC, the diploid strain produced 7.6 g/l ethanol in 72 hours, with an ethanol yield that achieved 75% of the theoretical value, and also produced 7.5 g/l ethanol from pretreated rice straw in 72 hours. Conclusions: We have developed diploid yeast strain optimized for expression of cellulolytic enzymes, which is capable of directly fermenting from cellulosic materials. Although this is a proof-of-concept study, it is to our knowledge, the first report of ethanol production from agricultural waste biomass using cellulolytic enzymeexpressing yeast without the addition of exogenous enzymes. Our results suggest that combining multigene expression optimization and diploidization in yeast is a promising approach for enhancing ethanol production from various types of lignocellulosic biomass.

    BioMed Central Ltd., 2011年, Biotechnology for Biofuels, 4 (1), 8, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

    YAMADA Ryosuke, TANIGUCHI N, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

    2011年, Biotechnology for Biofuels, 4:8, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazunori Nakashima, Koji Yamaguchi, Naho Taniguchi, Shogo Arai, Ryosuke Yamada, Satoshi Katahira, Nobuhiro Ishida, Haruo Takahashi, Chiaki Ogino, Akihiko Kondo

    The efficient production of biofuels from cellulosic biomass is a current imperative. The present study demonstrates direct bioethanol production from cellulose by cellulase-displaying yeast (arming yeast) using ionic liquid pretreatment. In this approach, all of the bioethanol production processes (pretreatment, saccharification, and fermentation) were conducted in a single vessel. In a preliminary test, the arming yeast showed resistance to cellulose-dissolving ionic liquids below a concentration of approximately 200 mM. Cellulose degradation and ethanol productivity by the arming yeast was dramatically enhanced by pretreatment with ionic liquids. The addition of free cellulases during fermentation significantly enhanced ethanol productivity in the arming yeast system, producing approximately 90% ethanol yield. Furthermore, in a preliminary examination, 82% of the ionic liquids in the culture medium could be recovered and recycled for the pretreatment of cellulose.

    ROYAL SOC CHEMISTRY, 2011年, GREEN CHEMISTRY, 13 (10), 2948 - 2953, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ninomiya Kazuaki, Oshima Shuhei, Sonoke Shiro, Ogino Chiaki, Kuroda Shun-ichi, Shimizu Nobuaki

    Our previous study suggested new sonodynamic therapy for cancer cells based on the delivery of titanium dioxide (TiO_2) nanoparticles (NPs) modified with a protein specifically recognizing target cells and subsequent generation of hydroxyl radicals from TiO_2 NPs activated by external ultrasound irradiation (called TiO_2/US treatment). The present study first examined the uptake behavior of TiO_2 NPs modified with pre-S1/S2 (model protein-recognizing hepatocytes) by HepG2 cells for 24 h. Next, the effect of the TiO_2/US treatment on HepG2 cell growth was examined for 96 h after the 1 MHz ultrasound was irradiated (0.1 W/cm^2, 30 s) to the cells which incorporated the TiO_2 NPs. Although no apparent cell-injury was observed until 24 h after the treatment, the viable cell concentration had deteriorated to 46% of the control at 96 h. Finally, the TiO_2/US treatment was applied to a mouse xenograft model. The pre-S1/S2-immobilized TiO_2 (0.1 mg) was directly injected into tumors, followed by 1 MHz ultrasound irradiation at 1.0 W/cm^2 for 60 s. As a result of the treatment repeated 5 times within 13 days, tumor growth could be hampered up to 28 days compared with the control conditions.

    日本ソノケミストリー学会, 2011年, ソノケミストリー討論会講演論文集, 20, 80 - 83, 英語

  • Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

    A yeast with the xylose isomerase (XI) pathway was constructed by the multicopy integration of XI overexpression cassettes into the genome of the Saccharomyces cerevisiae MT8-1 strain. The resulting yeast strain successfully produced ethanol from both xylose as the sole carbon source and a mixed sugar, consisting of xylose and glucose, without any adaptation procedure. Ethanol yields in the fermentation from xylose and mixed sugar were 61.9% and 62.2% of the theoretical carbon recovery, respectively. Knockout of GRE3, a gene encoding nonspecific aldose reductase, of the host yeast strain improved the fermentation profile. Not only specific ethanol production rates but also xylose consumption rates was improved more than twice that of xylose-metabolizing yeast with the XI pathway using GRE3 active yeast as the host strain. In addition, it was demonstrated that xylitol in the medium exhibits a concentration-dependent inhibition effect on the ethanol production from xylose with the yeast harboring the XI-based xylose metabolic pathway. From our findings, the combination of XI-pathway integration and GRE3 knockout could be result in a consolidated xylose assimilation pathway and increased ethanol productivity.

    SPRINGER, 2010年11月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88 (5), 1215 - 1221, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Construction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylITOl on fermentation

    Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohima, Akihiko Kondo

    2010年11月, Applied Microbiology and Biotechnology, 88, 1215-1221, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Noda, Yuko Ito, Nobuaki Shimizu, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    Streptomyces lividans is known to produce large amounts of proteins in culture supernatants. In this report, to expand the secretory expression system with a strong promoter derived from phospholipase D of Streptoverticillium cinnamoneum, we expressed three kinds of proteins: transglutaminase from Sty. cinnamoneum (StvcMTG) and beta-1,4-endoglucanase and beta-glucosidase from Thermobifida fusca YX. The StvcMTG gene was introduced into S. lividans using the shuttle vector pUC702 for Escherichia coli and S. lividans, and high level secretory production of StvcMTG (230 mu g/ml in the culture supernatant) was achieved. The other prokaryotic proteins, beta-1,4-endoglucanase and beta-glucosidase, were also expressed in (His)(6)-tag fused form into culture supernatants and retained high activity. Furthermore, complete purification was achieved by conventional column or affinity column chromatography for each recombinant protein with 1 mg/ml over protein concentration. Three independent proteins were thus successfully expressed and purified, and we expect to use this system for the expression of other valuable heterologous proteins. (C) 2010 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2010年10月, PROTEIN EXPRESSION AND PURIFICATION, 73 (2), 198 - 202, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takuya Shishido, Hiroaki Mieda, Sang Youn Hwang, Yuya Nishimura, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    A novel HER2-targeted carrier was developed using bionanocapsules (BNCs). Bionanocapsules (BNCs) are 100-nm hollow nanoparticles composed of the L-protein of hepatitis B virus surface antigen. An affibody of HER2 was genetically displayed on the BNC surface (Z(HER2)-BNC). For the investigation of binding affinity, Z(HER2)-BNC was incubated with the cancer cell lines SK-BR-3 (HER2 positive), and MDA-MB-231 (HER2 negative). For analysis of HER2 targeting specificity, Z(HER2)-BNC or Z(WT)-BNC (without affibody) was incubated with both SK-BR-3 and MDA-MB-231 cells by time lapse and concentration. For the delivery of encapsulated molecules (calcein), fluorescence of Z(HER2)-BNC mixed with liposomes was also compared with that of Z(WT)-BNC and nude liposomes by incubation with SK-BR-3 cells. As a result, Z(HER2)-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment. (C) 2010 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2010年10月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20 (19), 5726 - 5731, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Risa Koda, Takao Numata, Shinji Hama, Sriappareddy Tamalampudi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    We demonstrated ethanolysis of rapeseed oil to produce biodiesel fuel using lipase-producing filamentous fungi immobilized on biomass support particles (BSPs) as a whole-cell biocatalyst. We prepared two types of whole-cell biocatalyst: wild-type Rhizopus oryzae producing triacylglycerol lipase (w-ROL) and recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (r-FHL). Both w-ROL and r-FHL successfully catalyzed the ethanolysis of rapeseed oil, and the fatty acid ethyl ester yield was as high as 79% (w-ROL) or 94% (r-FHL). In the case of r-FHL, the residual monoglycerides (MGs) and diglycerides (DGs) were no more than 0.73 and 0.18%, respectively. In addition, r-FHL could be recycled for the ethanolysis of rapeseed oil, retaining over 85% fatty acid ethyl ester yield by the fifth cycle. r-FHL was revealed to be a promising catalyst for biodiesel production using rapeseed oil and ethanol. (C) 2010 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2010年09月, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 66 (1-2), 101 - 104, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Yanase, Tomohisa Hasunuma, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    To exploit cellulosic materials for fuel ethanol production, a microorganism capable of high temperature and simultaneous saccharification-fermentation has been required. However, a major drawback is the optimum temperature for the saccharification and fermentation. Most ethanol-fermenting microbes have an optimum temperature for ethanol fermentation ranging between 28 degrees C and 37 degrees C, while the activity of cellulolytic enzymes is highest at around 50 degrees C and significantly decreases with a decrease in temperature. Therefore, in the present study, a thermotolerant yeast, Kluyveromyces marxianus, which has high growth and fermentation at elevated temperatures, was used as a producer of ethanol from cellulose. The strain was genetically engineered to display Trichoderma reesei endoglucanase and Aspergillus aculeatus beta-glucosidase on the cell surface, which successfully converts a cellulosic beta-glucan to ethanol directly at 48 degrees C with a yield of 4.24 g/l from 10 g/l within 12 h. The yield ( in grams of ethanol produced per gram of beta-glucan consumed) was 0.47 g/g, which corresponds to 92.2% of the theoretical yield. This indicates that high-temperature cellulose fermentation to ethanol can be efficiently accomplished using a recombinant K. marxianus strain displaying thermostable cellulolytic enzymes on the cell surface.

    SPRINGER, 2010年09月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88 (1), 381 - 388, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiaki Ogino, Naonori Shibata, Ryosuke Sasai, Keiko Takaki, Yusuke Miyachi, Shun-ichi Kuroda, Kazuaki Ninomiya, Nobuaki Shimizu

    Recently, our group discovered an alternative titanium dioxide (TiO2) activation method that uses ultrasound irradiation (US/TiO2) instead of ultraviolet irradiation. The pre-S1/S2 protein from hepatitis B virus, which recognizes liver cells, was immobilized to the surface of TiO2 nanoparticles using an amino-coupling method. The ability of the protein-modified TiO2 nanoparticles to recognize liver cells was confirmed by surface plasmon resonance analysis and immuno-staining analyses. After uptake of TiO2 nanoparticles by HepG2 cancer cells, the cells were injured using this US/TiO2 method; significant cell injury was observed at an ultrasound irradiation intensity of 0.4 W/cm(2). Together with these results, this strategy could be applied to new cell injuring systems that use ultrasound irradiation in place of photodynamic therapy in the near future. (C) 2010 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2010年09月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20 (17), 5320 - 5325, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazusa Matsui, Miki Karasaki, Maiko Segawa, Sang Youn Hwang, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    Cancer cell-specific photokilling was successfully demonstrated using antibody-immobilized TiO2 nanoparticles with only 1 J cm(-2) UV irradiation.

    ROYAL SOC CHEMISTRY, 2010年09月, MEDCHEMCOMM, 1 (3), 209 - 211, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Fumiyoshi Okazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM and CBM-GFP) were expressed using A. oryzae as a host. Western blotting and fluorescence microscopy analysis showed that both GFP-CBM and CBM-GFP were successfully expressed on the cell surface. In addition, cell surface display of triacylglycerol lipase from A. oryzae (tglA), while retaining its activity, was also successfully demonstrated using CBM as an anchor protein. The activity of tglA was significantly higher when tglA was fused to the C terminus than N terminus of CBM. Together, these results show that CBM used as a first anchor protein enables the fusion of both the N and/or C terminus of a target protein.

    SPRINGER, 2010年08月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87 (5), 1783 - 1789, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    We successfully demonstrated batch ethanol fermentation repeated ten times from raw starch with high ethanol productivity. We constructed a yeast diploid strain coexpressing the maltose transporter AGT1, alpha-amylase, and glucoamylase. The introduction of AGT1 allows maltose and maltotriose fermentation as well as the improvement of amylase activities. We also found that alpha-amylase activity during fermentation was retained by the addition of 10 mM calcium ion and that the highest alpha-amylase activity was 9.26 U/ml during repeated fermentation. The highest ethanol productivity was 2.22 g/l/h at the fourth batch, and after ten cycles, ethanol productivity of more than 1.43 g/l/h was retained, as was alpha-amylase activity at 6.43 U/ml.

    SPRINGER, 2010年06月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87 (1), 109 - 115, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shota Togawa, Jun Ishii, Atsushi Ishikura, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    N-linked oligosaccharides or asparagine residues are often involved in G protein-coupled receptor functions. Focusing on Asn13 and Asn26 positioned on N-linked glycosylation motifs in the amino-terminal domain of human somatostatin receptor subtype-5 (hSSTR5), we performed site-directed mutagenesis and evaluated the mutants by using yeast cells as the host strain. This is because analysing the complicated signalling in mammalian cell lines is simplified by the utilization of the monopolistic pheromone signalling pathway in yeast. Western blot analysis and confocal laser scanning microscope observation showed that Asn13 and/or Asn26 mutations had no effects on cell-surface expression of hSSTR5 in yeast. By using an engineered yeast strain of Saccharomyces cerevisiae, which induces the expression of the green fluorescent protein (GFP) reporter gene in response to the agonist-specific signal transduction, it was demonstrated that a single mutation of two asparagine residues attenuated the somatostatin-specific signalling levels, and the double mutant significantly lost the signalling ability. These results clearly show the importance of these asparagine residues in the agonist-specific signalling of hSSTR5, although it was not enough to identify the consequence of oligosaccharides.

    OXFORD UNIV PRESS, 2010年06月, JOURNAL OF BIOCHEMISTRY, 147 (6), 867 - 873, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yusuke Iguchi, Jun Ishii, Hideki Nakayama, Atsushi Ishikura, Keiko Izawa, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    The yeast Saccharomyces cerevisiae is known as an available host for human G-protein-coupled receptor (GPCR) ligand screening. Although several types of yeast signal sequences (SS) attached with the GPCRs could improve their productivities and facilitate transportation of the GPCRs to the yeast plasma membrane, the effects of additional SS on ligand-specific signalling functions of GPCRs are not reported. Here, we demonstrated the controlling signalling properties by addition of SS using engineered yeast as a host. Prepro and pre regions of alpha-factor and amino-terminal sequence of Ste2 (Ste2N) were used as SS, and somatostatin (SST) receptor subtype-5 (SSTR5) was used as a model GPCR. We also constructed a yeast-based fluorescent assay system for monitoring the activation levels of SSTR5 signalling by a green fluorescent protein (GFP) reporter gene. The production levels and localisation patterns of the SS-attached SSTR5 were more significantly improved than those of wild-type SSTR5. In addition, we successfully controlled the pharmacological efficacy and potency by introducing SS. Among four types of SSTR5 receptors, Ste2N-SSTR5 responded at the lowest ligand concentration. This finding will be informative for constructing optimal yeast-based ligand screening systems to discriminate the cells on the basis of signalling levels.

    OXFORD UNIV PRESS, 2010年06月, JOURNAL OF BIOCHEMISTRY, 147 (6), 875 - 884, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Yanase, Ryosuke Yamada, Shohei Kaneko, Hideo Noda, Tomohisa Hasunuma, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    We demonstrate direct ethanol fermentation from amorphous cellulose using cellulase-co-expressing yeast. Endoglucanases (EG) and cellobiohydrolases (CBH) from Trichoderma reesei, and beta-glucosidases (BGL) from Aspergillus aculeatus were integrated into genomes of the yeast strain Saccharomyces cerevisiae MT8-1. BGL was displayed on the yeast cell surface and both EG and CBH were secreted or displayed on the cell surface. All enzymes were successfully expressed on the cell surface or in culture supernatants in their active forms, and cellulose degradation was increased 3- to 5-fold by co-expressing EG and CBH. Direct ethanol fermentation from 10 g/L phosphoric acid swollen cellulose (PASC) was also carried out using EG-, CBH-, and BGL-co-expressing yeast. The ethanol yield was 2.1 g/L for EG-, CBH-, and BGL-displaying yeast, which was higher than that of EG- and CBH-secreting yeast (1.6 g/L ethanol). Our results show that cell surface display is more suitable for direct ethanol fermentation from cellulose.

    WILEY-V C H VERLAG GMBH, 2010年05月, BIOTECHNOLOGY JOURNAL, 5 (5), 449 - 455, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMADA Ryosuke, TANIGUCHI, N, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

    Background: The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as beta-glucosidase, endoglucanase, and cellobiohydrolase. The expression levels of each cellulase are controlled simultaneously, and their ratios and synergetic effects are important for effective cellulose degradation. However, in recombinant Saccharomyces cerevisiae, it is difficult to simultaneously control many different enzymes. To construct engineered yeast with efficient cellulose degradation, we developed a simple method to optimize cellulase expression levels, named cocktail delta-integration. Results: In cocktail delta-integration, several kinds of cellulase expression cassettes are integrated into yeast chromosomes simultaneously in one step, and strains with high cellulolytic activity (i.e., expressing an optimum ratio of cellulases) are easily obtained. Although the total integrated gene copy numbers of cocktail delta-integrant strain was about half that of a conventional delta-integrant strain, the phosphoric acid swollen cellulose (PASC) degradation activity (64.9 mU/g-wet cell) was higher than that of a conventional strain (57.6 mU/g-wet cell). This suggests that optimization of the cellulase expression ratio improves PASC degradation activity more so than overexpression. Conclusions: To our knowledge, this is the first report on the expression of cellulase genes by delta-integration and optimization of various foreign genes by delta-integration in yeast. This method should be very effective and easily applied for other multi-enzymatic systems using recombinant yeast.

    BIOMED CENTRAL LTD, 2010年05月, Microbial Cell Factories, 9, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mohammad Mizanur Rahman, Kazuaki Ninomiya, Chiaki Ogino, Nobuaki Shimizu

    A non-woven titanium dioxide (TiO2) fabric was applied to disinfection by ultrasound (US) irradiation, and the disinfection efficiency and lipid peroxidation of Escherichia coli (E. coli) cell membrane were evaluated to investigate the killing process The addition of non-woven TiO2 fabric enhanced hydroxyl (OH) radical generation and disinfection efficiency Judging from the disinfection experiments using glutathione or t-butanol as a radical scavenger, the OH radical played a major role in cell killing in sonodynamic disinfection with non-woven TiO2 fabric Moreover, to understand the detailed killing process, damage to cell membrane was also evaluated using a diphenyl-l-pyrenylphosphine (DPPP) fluorescent probe, which detects the membrane's lipid peroxidation The addition of non-woven TiO2 fabric aggravated this peroxidation This aggravation was caused by the OH radical according to an assay using a radical scavenger From these results, it was concluded that non-woven no, fabric as a sonocatalyst promoted peroxidation of the polyunsaturated phospholipid component of the lipid membrane initially and induced a major disorder in the E. coli cell membrane under US irradiation. (C) 2009 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2010年04月, ULTRASONICS SONOCHEMISTRY, 17 (4), 738 - 743, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Akihiko Kondo

    Atomic force microscopy (AFM) can detect the adhesion or affinity force between a sample surface and cantilever, dynamically. This feature is useful as a method for the selection of aptamers that bind to their targets with very high affinity. Therefore, we propose the Systematic Evolution of Ligands by an EXponential enrichment (SELEX) method using AFM to obtain aptamers that have a strong affinity for target molecules. In this study, thrombin was chosen as the target molecule, and an 'AFM-SELEX' cycle was performed. As a result, selected cycles were completed with only three rounds, and many of the obtained aptamers had a higher affinity to thrombin than the conventional thrombin aptamer. Moreover, one type of obtained aptamer had a high affinity to thrombin as well as the anti-thrombin antibody. AFM-SELEX is, therefore, considered to be an available method for the selection of DNA aptamers that have a high affinity for their target molecules.

    OXFORD UNIV PRESS, 2010年03月, NUCLEIC ACIDS RESEARCH, 38 (4), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Atsushi Kotaka, Hiroshi Sahara, Tsuneharu Hashimoto, Nobuyuki Kuratani, Seiji Shibasaki, Chiaki Ogino, Hideo Noda, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

    We used cutinase from the filamentous fungi Aspergillus oryzae to produce dairy flavors. Secretory and displayed forms of cutinase were investigated using salt-free butter, which is composed mostly of triacylglycerides, as the substrate. The secretory form of cutinase, which was produced in recombinant A. oryzae, was suitable for producing butyric acids (16.8 mol%). Also, cutinase displayed on the cell surface of the yeast Saccharomyces cerevisiae as a fusion protein with alpha-agglutinin released butyric acid at a 2.7-fold rate (45.4 mol%) higher than that of the secreted form. Yeasts carrying two copies of cutinase genes into their chromosomes, which were constructed using the HELOH method, released free fatty acids rapidly and showed 2-fold higher lipase activity compared with yeasts carrying one copy of the cutinase gene. (C) 2010 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2010年03月, ENZYME AND MICROBIAL TECHNOLOGY, 46 (3-4), 194 - 199, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Shimizu, Kazuaki Ninomiya, Chiaki Ogino, Mohammad Mizanur Rahman

    The inactivation of hazardous microorganisms is an important subject for water treatment. While the most widely used methods in water treatment systems are chlorination, ozonation, and ultraviolet ray irradiation, the drawbacks of these techniques surpass their efficacy. The potential formation of carcinogenic chemical byproducts has been reported as a drawback of chemical disinfection methods, hence there is a need to develop some alternate disinfection techniques. Ultrasonic (US) irradiation is well known as a useful technique for microbial inactivation due to its chemical and physical factors. Our recent studies indicated that the presence of titanium dioxide (TiO2), known as a photocatalyst, accelerates the generation of hydroxyl (OH) radicals during US irradiation, and that the process is mediated through the induction of cavitation bubbles in irradiating solutions. This review describes a disinfection method that utilizes US irradiation of contaminated solution in the presence of TiO2, a so-called "sonocatalytic disinfection" method. The significant role of OH radicals in the mechanism of cell-killing is discussed. © 2009 Elsevier B.V. All rights reserved.

    2010年02月15日, Biochemical Engineering Journal, 48 (3), 416 - 423, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMADA, Ryosuke, TANAKA, Tsutomu, OGINO, Chiaki, FUKUDA, Hideki, KONDO, Akihiko

    We developed a novel strategy for constructing yeast to improve levels of amylase gene expression and the practical potential of yeast by combining delta-integration and polyploidization through cell fusion. Streptococcus bovis alpha-amylase and Rhizopus oryzae glucoamylase/alpha-agglutinin fusion protein genes were integrated into haploid yeast strains. Diploid strains were constructed from these haploid strains by mating, and then a tetraploid strain was constructed by cell fusion. The alpha-amylase and glucoamylase activities of the tetraploid strain were increased up to 1.5- and tenfold, respectively, compared with the parental strain. The diploid and tetraploid strains proliferated faster, yielded more cells, and fermented glucose more effectively than the haploid strain. Ethanol productivity from raw starch was improved with increased ploidy; the tetraploid strain consumed 150 g/l of raw starch and produced 70 g/l of ethanol after 72 h of fermentation. Our strategy for constructing yeasts resulted in the simultaneous overexpression of genes integrated into the genome and improvements in the practical potential of yeasts.

    SPRINGER, 2010年02月, Applied Microbiology and Biotechnology,, 85 (5), 1491 - 1498, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shogo Arai, Kazunori Nakashima, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

    The methanolysis of soybean oil to produce a fatty acid methyl ester (ME, i.e., biodiesel fuel) was catalyzed by lipase-producing filamentous fungi immobilized on biomass support particles (BSPs) as a whole-cell biocatalyst in the presence of ionic liquids. We used four types of whole-cell biocatalysts: wildtype Rhizopus oryzae producing triacylglycerol lipase (w-ROL), recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (r-FHL), Candida antarctica lipase B (r-CALB), and mono- and diacylglycerol lipase from A. oryzae (r-mdlB). w-ROL gave the high yield of fatty acid methyl ester (ME) in ionic liquid [Emim][BF(4)] or [Bmim][BF(4)] biphasic systems following a 24h reaction. While lipases are known to be severely deactivated by an excess amount of methanol (e.g. 1.5 Mequiv. of methanol against oil) in a conventional system, methanolysis successfully proceeded even with a methanol/oil ratio of 4 in the ionic liquid biphasic system, where the ionic liquids would work as a reservoir of methanol to suppress the enzyme deactivation. When only w-ROL was used as a biocatalyst for methanolysis, unreacted monoglyceride remained due to the 1,3-positional specificity of R. oryzae lipase. High ME conversion was attained by the combined use of two types of whole-cell biocatalysts, w-ROL and r-mdlB. In a stability test, the activity of w-ROL was reduced to one-third of its original value after incubation in [Bmim][BF(4)] for 72 h. The stability of w-ROL in [Bmim][BF(4)] was greatly enhanced by cross-linking the biocatalyst with glutaraldehyde. The present Study demonstrated that ionic liquids are promising candidates for use as the second solvent in biodiesel fuel production by whole-cell biocatalysts. (C) 2009 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2010年01月, ENZYME AND MICROBIAL TECHNOLOGY, 46 (1), 51 - 55, 英語

    [査読有り]

    研究論文(学術雑誌)

  • OKANO, K, ZHANG, Q, YOSHIDA, Shogo, TANAKA, Tsutomu, OGINO, Chiaki, FUKUDA, Hideki, KONDO, Akihiko

    In order to achieve direct fermentation of an optically pure d-lactic acid from cellulosic materials, an endoglucanase from a Clostridium thermocellum (CelA)-secreting plasmid was introduced into an l-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum (a dagger ldhL1) bacterial strain. CelA expression and its degradation of beta-glucan was confirmed by western blot analysis and enzyme assay, respectively. Although the CelA-secreting a dagger ldhL1 assimilated cellooligosaccharides up to cellohexaose (although not cellotetraose), the main end product was acetic acid, not lactic acid, due to the conversion of lactic acid to acetic acid. Cultivation under anaerobic conditions partially suppressed this conversion resulting in the production of 1.27 g/l of D-lactic acid with a high optical purity of 99.5% from a medium containing 2 g/l of cellohexaose. Subsequently, D-lactic acid fermentation from barley beta-glucan was carried out with the addition of Aspergillus aculeatus beta-glucosidase produced by recombinant Aspergillus oryzae and 1.47 g/l of D-lactic was produced with a high optical purity of 99.7%. This is the first report of direct lactic acid fermentation from beta-glucan and a cellooligosaccharide that is a more highly polymerized sugar than cellotriose.

    SPRINGER, 2010年01月, Applied Microbiology and Biotechnology, 85 (3), 643 - 650, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Lactic acid (LA) is an important and versatile chemical that can be produced from renewable resources such as biomass. LA is used in the food, pharmaceutical, and polymers industries and is produced by microorganism fermentation; however, most microorganisms cannot directly utilize biomass such as starchy materials and cellulose. Here, we summarize LA production using several kinds of genetically modified microorganisms, such as LA bacteria, Escherichia coli, Corynebacterium glutamicum, and yeast. Using gene manipulation and metabolic engineering, the yield and optical purity of LA produced from biomass has been significantly improved. In this review, the drawbacks as well as improvements of LA production by fermentation is discussed.

    SPRINGER, 2010年01月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85 (3), 413 - 423, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomonori Ito, Atsushi Hotta, Atsuko Uchida, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

    Glucose and xylose are major component of the lignocellulosic biomass, and the efficient utilization of not only glucose but also xylose has been required for large-scale ethanol production. In order to establish a highly efficient bio-ethanol production process, this study tried to enhance xylose asimilation ability by introducing the metabolic pathway. Xylose isomerase (XI) pathway (called Pathway I in this study) was selected as a xylose metabolic pathway, and introduced into Saccharomyces cerevisiae genome by integration of XI expression cassette. The resulting recombinant yeast (MT8-1/XKδXI) attained successfully production of ethanol from not only xylose as the sole carbon source but also mixed sugar of xylose and glucose. The xylose consumption rate of the fermentation from xylose was estimated to be a second-order reaction model, and from mixed sugars was estimated to be a first-order reaction model, respectively. These results were expected to be a glucose effect on fermentation from xylose. Copyright © 2010 AIDIC Servizi S.r.l.

    Italian Association of Chemical Engineering - AIDIC, 2010年, Chemical Engineering Transactions, 20, 103 - 108, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Tomonori Ito, Atsushi Hotta, Atsuko Uchida, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

    Glucose and xylose are major component of the lignocellulosic biomass, and the efficient utilization of not only glucose but also xylose has been required for large-scale ethanol production. In order to establish a highly efficient bio-ethanol production process, this study tried to enhance xylose asimilation ability by introducing the metabolic pathway. Xylose isomerase (XI) pathway (called Pathway I in this study) was selected as a xylose metabolic pathway, and introduced into Saccharomyces cerevisiae genome by integration of XI expression cassette. The resulting recombinant yeast (MT8-1/XK delta XI) attained successfully production of ethanol from not only xylose as the sole carbon source but also mixed sugar of xylose and glucose. The xylose consumption rate of the fermentation from xylose was estimated to be a second-order reaction model, and from mixed sugars was estimated to be a first-order reaction model, respectively. These results were expected to be a glucose effect on fermentation from xylose.

    AIDIC SERVIZI SRL, 2010年, IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY, 20, 103 - 108, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Kenji Okano, Shogo Yoshida, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    The production of optically pure D-lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose L-lactate dehydrogenase gene was deficient and whose phosphoketolase genes were replaced with a heterologous transketolase gene. After 60 h of fermentation, 41.2 g/liter of D-lactic acid was produced from 50 g/liter of xylose.

    AMER SOC MICROBIOLOGY, 2009年12月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75 (24), 7858 - 7861, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takuya Shishido, Yuki Azumi, Takeshi Nakanishi, Mitsuo Umetsu, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Bionanocapsule (BNC) is hollow nanoparticle composed of the l-protein of the hepatitis B virus surface antigen. BNC allows targeted delivery of either genes or drugs only to hepatocytes, but not to other cell types. In this study, we attempted to alter the specificity of BNC by insertion of biotin-acceptor peptide (BAP), which is efficiently biotinylated using biotin ligase BirA from Escherichia coli. Using streptavidin as a linker, biotinylated BNC could be display various biotinylated ligands that are otherwise difficult to fuse with BNC, such as antibodies, synthetic peptides and functional molecules. BAP-fused BNC was efficiently biotinylated and effectively displayed streptavidin. Furthermore, we demonstrated that biotinylated BNC was internalized into targeted cells via biotinylated Nanobody displayed on the BNC surface. Biotinylated BNC permit display of diverse ligands, and thus have potential as a versatile carrier for drug delivery to a variety of target cells.

    OXFORD UNIV PRESS, 2009年12月, JOURNAL OF BIOCHEMISTRY, 146 (6), 867 - 874, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takanori Tanino, Takumi Ohm, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    The effect of the reaction conditions on the ester synthesis reaction with CALB displaying yeast whole cells was determined. Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the ester synthesis activity of CALB displaying yeast whole cells. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108 (5), 369 - 371, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Optically pure D-lactic acid fermentation from arabinose was achieved by using the Lactobacillus plantarum NCIMB 8826 strain whose L-lactate dehydrogenase gene was deficient and whose phosphoketolase gene was substituted with a heterologous transketolase gene. After 27 h of fermentation, 38.6 g/liter of D-lactic acid was produced from 50 g/liter of arabinose.

    AMER SOC MICROBIOLOGY, 2009年08月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75 (15), 5175 - 5178, 英語

    [査読有り]

    研究論文(学術雑誌)

  • HORII, Ken, ADACHI, T, MATSUDA, T, TANAKA, Tsutomu, SAHARA, H, SHIBASAKI, S, OGINO, Chiaki, HATA, Y, UEDA, M, KONDO, Akihiko

    beta-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than P-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides. (C) 2008 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2009年08月, Journal of Molecular Catalysis B: ,, 59 (4), 297 - 301, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

    A molecular display technology that uses the displayed proteins on cell surfaces has many applications in microbiology and molecular biology. Here, we describe the resistance of displayed proteins to proteases using simulated gastric fluid (SGF), which included pepsin at pH 2. The displayed beta-glucosidase resisted pepsin digestion compared with secreted, free beta-glucosidase. In SDS-PAGE and Western blotting analysis, the secreted beta-glucosidase was immediately digested within 1 min following SGF treatment, although the displayed beta-glucosidase was stable for more than 60 min following SGF treatment. In addition, the residual activity of secreted beta-glucosidase was completely destroyed after 10 min SGF treatment. However, displayed beta-glucosidase retained 14% of its residual activity following the same treatment. These results clearly show that cell surface display technology using enzymes can reveal the protease resistance of a protein of interest under various conditions.

    SPRINGER, 2009年08月, BIOTECHNOLOGY LETTERS, 31 (8), 1259 - 1264, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). However, 8-OHdG was difficult to immobilize on a carrier for SELEX. Therefore, a DNA aptamer binding to 8-OHdG was selected using GMP-agarose as an analogue from a library of about 4(60) random ssDNA sources. As a result, three aptamer candidates were selected. Among the selected DNA aptamers, the No. 22 DNA aptamer exhibited a high affinity for 8-OHdG. The dissociation constant, K(D), of No. 22 DNA aptamer was on the order of 0.1 mu mol/L. This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix. (C) 2009 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2009年07月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19 (13), 3619 - 3622, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Junji Ito, Akihiko Kosugi, Tsutomu Tanaka, Kouichi Kuroda, Seiji Shibasaki, Chiaki Ogino, Mitsuyoshi Ueda, Hideki Fukuda, Roy H. Doi, Akihiko Kondo

    We constructed a novel cell surface display system to control the ratio of target proteins on the Saccharomyces cerevisiae cell surface, using two pairs of protein-protein interactions. One protein pair is the Z domain of protein A derived from Staphylococcus aureus and the Fc domain of human immunoglobulin G. The other is the cohesin (Coh) and dockerin (Dock) from the cellulosome of Clostridium cellulovorans. In this proposed displaying system, the scaffolding proteins (fusion proteins of Z and Coh) were displayed on the cell surface by fusing with the 3' half of alpha-agglutinin, and the target proteins fused with Fc or Dock were secreted. As a target protein, a recombinant Trichoderma reesei endoglucanase II (EGII) was secreted into the medium and immediately displayed on the yeast cell surface via the Z and Fc domains. Display of EGII on the cell surface was confirmed by hydrolysis of beta-glucan as a substrate, and EGII activity was detected in the cell pellet fraction. Finally, two enzymes, EGII and Aspergillus aculeatus beta-glucosidase 1, were codisplayed on the cell surface via Z-Fc and Dock-Coh interactions, respectively. As a result, the yeast displaying two enzymes hydrolyzed beta-glucan to glucose very well. These results strongly indicated that the proposed strategy, the simultaneous display of two enzymes on the yeast cell surface, was accomplished by quantitatively controlling the display system using affinity binding.

    AMER SOC MICROBIOLOGY, 2009年06月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75 (12), 4149 - 4154, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jun Ishii, Keiko Izawa, Shizuka Matsumura, Kanako Wakamura, Takanori Tanino, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    To allow the comprehensive assessments of yeast expression systems, a simple and immediate method for simultaneously evaluating the expression level and plasmid maintenance in yeast was demonstrated. This method uses green fluorescent protein (GFP) and flow cytometry (FCM) and is characterized by a dual analysis of the average intensity of GFP fluorescence and the population of GFP-expressing cells. The FCM analysis of GFP fluorescence intensity rapidly quantifies the expression level without complex manipulations, such as the enzymatic reaction of a lacZ reporter assay. Moreover, the single-cell analysis revealed that the proportion of cells expressing GFP in the cell cluster reflects the plasmid retention rate; therefore, the FCM analysis of the GFP-expressing population allows the immediate estimation of the plasmid retention rate without the 2- or 3-day incubation required for colony counting. We show that the FCM analysis with GFP reporter is a suitable method to explore the hopeful expression vector and host strain or establish the several expression systems exhibiting the characteristic properties in yeast.

    OXFORD UNIV PRESS, 2009年06月, JOURNAL OF BIOCHEMISTRY, 145 (6), 701 - 708, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMADA, Ryosuke, BITO, Y, ADACHI, T, TANAKA, Tsutomu, OGINO, Chiaki, FUKUDA, Hideki, KONDO, Akihiko

    The goal of this research was to construct a stable and efficient process for the production of ethanol from raw starch, using a recombinant Saccharomyces cerevisiae. which is productive even under conditions such as non-selection or long-term operation. Three recombinant yeast strains were used, two haploid strains (MT8-1SS and NBRC1440SS) and one diploid strain (MN8140SS). The recombinant strains were constructed by integrating the glucoamylase gene from Rhizopus oryzae fused with the 3'-half of the alpha-agglutinin gene as the anchor protein, and the alpha-amylase gene from Streptococcus bovis, respectively, into their chromosomal DNA by homologous recombination. The diploid strain MN8140SS was constructed by mating these opposite types of integrant haploid strains in order to enhance the expression of integrated amylase genes. The diploid strain had the highest ethanol productivity and reusability during fermentation from raw starch. Moreover, the ethanol production rate of the integrant diploid strain was maintained when batch fermentation was repeated three times (0.67. 0.60, and 0.67 g/l/h in each batch). These results clearly show that a diploid strain developed by mating two integrant haploid strains is useful for the establishment of an efficient ethanol production process. (C) 2009 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2009年05月, Enzyme and Microbial Technology, 44 (5), 344 - 349, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takuya Shishido, Daisaku Yonezawa, Kiyokazu Iwata, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Bionanocapsule (BNC) is a hollow nanoparticle composed of L-protein of the hepatitis B virus surface antigen. BNC can deliver genes or drugs into specific human hepatocytes, but delivery is limited to hepatocytes. In this study, we attempted to alter the specificity of BNCs by genetically introducing cell-penetrating peptides (CPPs), such as arginine-rich peptides, into BNCs. The CPP-fused BNC was efficiently internalized into various cell lines in a short period without significant cytotoxicity. These results show that CPP-BNC could be applied as an efficient carrier for gene and drug delivery. (C) 2009 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2009年03月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19 (5), 1473 - 1476, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takanori Tanino, Tohru Aoki, Won-young Chung, Yuka Watanabe, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    A Candida antarctica lipase B (CALB)-displaying yeast whole-cell biocatalyst was constructed with the integration of the CALB cell-surface display expression cassette in the yeast genome and cell fusion by mating. Lipase hydrolytic activity of the yeast whole-cell biocatalyst subsequently increased, in both a- and alpha-type yeast cells, with the number of copies of the CALB cell-surface display expression cassette introduced, and reached 43.6 and 32.2 U/g-dry cell at 168 h cultivation, respectively. The lipase hydrolytic activity of whole cells in diploid yeast cells containing eight copies of the CALB cell-surface expression cassette reached 117 U/g-dry cell, and this value is approximately ninefold higher than that of the previously reported haploid CALB cell-surface displaying yeast using a multi-copy plasmid (Tanino et al. Appl. Microbial Biotechnol 75:1319-1325, 2007). This improved novel CALB-displaying yeast whole-cell biocatalyst could repeatedly catalyze the polyester, polybutylene adipate, synthesis reaction, using adipic acid and 1, 4-butandiol as the monomer molecules, four times in succession. This is the first report of the polymer synthesis using enzyme displaying yeast as the catalyst. The ratios of cyclic compounds in the polybutylene adipates synthesized with the CALB-displaying yeast whole-cells were lower than that in the polybutylene adipate synthesized with conventional metal catalysis. From these results, it appears that the use of CALB-displaying yeast cells could be useful for the polyester synthesis reaction, with reduced by-product production.

    SPRINGER, 2009年02月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82 (1), 59 - 66, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

    公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 912 - 912, 日本語

  • 大島 周平, 仁宮 一章, 荻野 千秋, 黒田 俊一, 清水 宣明

    Titanium dioxide (TiO_2) is known as sonocatalyst which generate hydroxyl radicals when the ultrasonic (US) is irradiated to TiO_2 (TiO_2/ US method). In this study, we investigated the cell damage effect and antitumor effect by the combination of ultrasonic irradiation and TiO_2 nanoparticles that is modified with preSl/S2 protein for targeting HepG2 cells. For irradiating of US to the preS1/S2-TiO_2 nanoparticles incorporated HepG2, cell injuring efficiency was evaluated by Trypan blue dye exclusion test. As a result, the cytostatic activity was acquired when irradiated at 1MHz, 0.1 W/ cm^2, 30 second, 50% duty in the presence of TiO_2 nanoparticle modified with preS1/S2 protein. This cytostatic activity was observed at 24 hour after US irradiation, and this effect was significantly enhanced as time elapsed. Also, antitumor effect by TiO_2 / U.S. method in vivo was evaluated. When ultrasonic irradiation was performed by the frequency of 3 times per week, and the irradiation conditions of 1 MHz, 1 W/cm^2, 1 min, no significant damages to the mice was ovserved. These irradiation condition revealed the antitumor effect significantly. Moreover the further research, such as the examination of the irradiation conditions of ultrasound, injection amount of TiO_2 nanoparticles and the specific binding ability to HepG2 tumor after i.v. injection, are proposed.

    日本ソノケミストリー学会, 2009年, ソノケミストリー討論会講演論文集, 18, 5 - 6, 日本語

  • 堀田 淳史, 伊藤 友教, 内田 敦子, 谷野 孝徳, 荻野 千秋, 大村 直人

    公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 242 - 242, 日本語

  • Naoya Kurata, Takuya Shishido, Masaru Muraoka, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating enhanced green fluorescent protein (EGFP) inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-epidermal growth factor receptor (EGFR) antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery.

    OXFORD UNIV PRESS, 2008年12月, JOURNAL OF BIOCHEMISTRY, 144 (6), 701 - 707, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashi Adachi, Junji Ito, Kouji Kawata, Masahiro Kaya, Hiroki Ishida, Hiroshi Sahara, Yoji Hata, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    A novel cell-surface display system was constructed in Aspergillus oryzae. Each of the five genes encoding the putative cell-wall-localized protein from the A. oryzae genome was cloned and these cell-surface anchor functions were examined by fusion to the C-terminal of the green fluorescent protein (GFP). Using the MP1 and CWP proteins as anchor proteins, GFP signals were strongly observed on the cell surface of recombinant A. oryzae. When these proteins were used as anchor proteins for cell-surface display of beta-glucosidase from A. oryzae, enzyme activity was detected on the cell surface. In particular, beta-glucosidase activity of recombinant A. oryzae using MP1, a putative glycosylphosphatidylinositol (GPI) anchor protein was higher than CWP. Based on these results, it was concluded that the MP1 protein can act as a GPI-anchor protein in A. oryzae, and the proposed cell-surface display system using MP1 allows for the display of heterogeneous and endogenous proteins.

    SPRINGER, 2008年12月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81 (4), 711 - 719, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Shimizu, Chiaki Ogino, Mahmoud Farshbaf Dadjour, Kazuaki Ninomiya, Atsushi Fujihira, Kazumichi Sakiyama

    The generation of hydroxyl (CH) radicals was investigated during ultrasonic irradiation and in the presence of TiO(2). The effect of TiO(2) on an ultrasonic system's oxidation power was evaluated by examining the oxidation of salicylic acid. The generation of the salicylic acid derivatives, 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA, was measured by high-performance liquid chromatography coupled with electro-chemical detection under different experimental conditions. The presence of TiO(2) enhanced the generation of DHBA during ultrasonic irradiation, thus indicating a higher oxidation power in the ultrasonic system. Al(2)O(3) also increased the generation of DHBA during irradiation; however, the effect of TiO(2) was found to be higher than that of Al(2)O(3). The addition of OH radical scavengers such as dimethylsulfoxide (DMSO), methanol and mannitol significantly suppressed the production of DHBA, and DMSO was found to have the highest suppressive effect among all scavengers. The effects of dissolved gases on the generation of OH radicals were further studied, and their power was found to be in the order Xe > At > O(2) > N(2). The degassing of the irradiation solution completely suppressed the generation of CH radicals. These results indicate that the presence of TiO(2) accelerates the generation of OH radicals during ultrasonic irradiation, and that the process may be mediated through the induction of cavitation bubbles in irradiating solutions. (c) 2008 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2008年09月, ULTRASONICS SONOCHEMISTRY, 15 (6), 988 - 994, 英語

    [査読有り]

    研究論文(学術雑誌)

  • ITO J, SAHARA H, KAYA M, HATA Y, SHIBASAKI S, KAWATA K, ISHIDA S, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

    The yeast Saccharomyces cerevisiae GRI-117-UK was transformed to either display or secrete beta-glucosidase 1 (BGL1) from the koji mold, Aspergillus oryzae. The P-glucosidase activity of BGL1-displaying yeast strains reached 405.9 U/g dry cell mass after 72 h of cultivation in YPD medium. The optimal pH and temperature for BGL1 displayed on the cell surfaces of the yeast were 5.0 and 55 degrees C, while the optima for BCL1 secreted by the yeast were 4.0 and 55 degrees C. The displayed BGL1 was stable at higher pH compared with the secreted BGL1. In addition, the thermostability of BGL1 was improved by displaying the enzyme on the yeast cell surfaces. In addition, the displayed and secreted forms of BGL1 had similar substrate specificity. beta-Glucosidase hydrolyzes daidzin and genistin, which are the glycoside forms of soybean isoflavones, to the aglycones. Isoflavone aglycones were efficiently produced by BGL1-displaying yeast from an isoflavone mixture: at optimal temperature and pH the rate of aglycone production was at least 15.8 g/(1h). After 144 h of reaction, almost isoflavones were converted to its aglycone by BGL1-displaying yeast. The results of the present study demonstrate that BGL1-displaying yeast strains are effective whole cell biocatalysts of isoflavone aglycone production. (c) 2008 Elsevier B. V. All rights reserved.

    ELSEVIER SCIENCE BV, 2008年09月, Journal of Molecular Catalysis B, 55 (1-2), 69 - 75, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Bioethanol Production from Sugars by Yeast Having Sugar Uptake Ability by Expression of Sugar Transporter

    TANINO Takanori, OGINO Chiaki, KUMAGAI Norihisa, OHMURA Naoto

    2008年09月, Proceedings of the 10th International Chemical and Biological Engineering Conference-CHEMPOR 2008, Braga, Portugal (on CD-ROM), E20, 4pages, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Properties of TiO2-Polyacrylic acid (PAA) Dispersions having Molecular Recognition Ability

    KANEHIRA K, BANZAI T, OGINO Chiaki, SHIMIZU N, KUBOTA Y, SONEZAKI S

    2008年06月, Colloids and Surfaces B: Biointerfaces, Vol 64, pp. 10-15, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Jun Ishii, Tsutomu Tanaka, Shizuka Matsumura, Kenji Tatematsu, Shun'Ichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    Here, we describe a yeast-based fluorescence reporter assay for G protein-coupled receptor (GPCR) signalling using a flow cytometer (FCM). The enhanced green fluorescent protein (EGFP) gene was integrated into the FUS1 locus as a reporter gene. The engineered yeast was able to express the EGFP in response to ligand stimulation. Gene-disrupted yeast strains were constructed to evaluate the suitability of the yeast-based fluorescence screening system for heterologous GPCR. When receptor was expressed by episomal plasmid, the proportion of the signalling-activated cells in response to ligand stimulation decreased significantly. The GPCR-signalling-activated and non-activated cell clusters were individually isolated by analysing the fluorescence intensity at the single-cell level with FCM, and it was found that the plasmid retention rate decays markedly in the non-activated cell cluster. We attributed the loss of plasmid to G1 arrest in response to signalling, and successfully improved the plasmid retention rate by disrupting the FAR1 gene and avoiding cell cycle arrest. Our system will be a powerful tool for the quantitative and high-throughput GPCR screening of yeast-based combinatorial libraries using FCM. © 2008 The Japanese Biochemical Society.

    2008年05月, Journal of Biochemistry, 143 (5), 667 - 674, 英語

    [査読有り]

    研究論文(学術雑誌)

  • KAYA M, ITO J, KOTAKA A, MATSUMURA K, BANDO H, SAHARA H, OGINO Chiaki, SHIBASAKI S, KURODA K, UEDA M, KONDO Akihiko, HATA Y

    For efficient production of isoflavone aglycones from soybean isoflavones, we isolated three novel types of beta-glucosidase (BGL1, BGL3, and BGL5) from the filamentous fungi Aspergillus oryzae. Three enzymes were independently displayed on the cell surface of a yeast Saccharomyces cerevisiae as a fusion protein with alpha-agglutinin. Three beta-glucosidase-displaying yeast strains hydrolyzed isoflavone glycosides efficiently but exhibited different substrate specificities. Among these beta-glucosidases, BGL1 exhibited the highest activity and also broad substrate specificity to isoflavone glycosides. Although glucose released from isoflavone glycosides are generally known to inhibit beta-glucosidase, the residual ratio of isoflavone glycosides in the reaction mixture with BGL1-displaying yeast strain (Sc-BGL1) reached approximately 6.2%, and the glucose concentration in the reaction mixture was maintained at lower level. This result indicated that Sc-BGL1 assimilated the glucose before they inhibited the hydrolysis reaction, and efficient production of isoflavone aglycones was achieved by engineered yeast cells displaying beta-glucosidase.

    SPRINGER, 2008年05月, Applied Microbiology and Biotechnology, 79 (1), 51 - 60, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiaki Ogino, Mahmoud Farshbaf Dadjour, Yasuo Iida, Nobuaki Shimizu

    The pretreatment of TiO2-photocatalysts in solutions of H2O2 was studied by examining the decolorization of methylene blue in the dark. Incubation of TiO2 particles in H2O2 solutions increased the oxidizing capacity of TiO2. Methylene blue (0.3 mM) was degraded in the presence of pretreated TiO2, and a decolorizing ratio of 47% was obtained after a 48-h incubation period in the presence of 5.0 g/L pretreated TiO2. Titanium peroxide as a stable oxidant, which can be synthesized with the reaction of titanium sulfate and H2O2, was studied in the decolorizing process of methylene blue. Concentrations of methylene blue were significantly reduced in the presence of titanium peroxide, and a greater extent of decolorization was obtained with larger amounts of titanium peroxide. A 63% decrease in methylene blue concentration was achieved in 5 h incubation in the presence of 4.0 g/L titanium peroxide. H2O2 accelerated the decolorizing process in the presence of titanium peroxide. The addition of 100 mM H2O2 to a methylene blue solution containing 2.0 g/L titanium peroxide increased the decolorizing ratio to 85% after 5 h incubation. The addition of a hydroxyl radical scavenger, dimethyl sulfoxide, significantly decreased the decolorizing ratio, indicating the role of hydroxyl radicals in the oxidation process. (c) 2007 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2008年05月, JOURNAL OF HAZARDOUS MATERIALS, 153 (1-2), 551 - 556, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Growth acceleration of plants and mushroom by erythritol

    Masahiko Kaya, Junji Ito, Atsushi Kotaka, Kengo Matsumura, Hiroki Bando, Hiroshi Sahara, Chiaki Ogino, Seiji Shibasaki, Kouichi Kuroda, Mitsuyoshi Ueda, Akihiko Kondo, Yoji Hata

    2008年, Appl. Microbiol. Biotechnol., 79: 51-60, 英語

    [査読有り]

  • 荒川 雅幸, ラハマン モハマド ミザヌル, 仁宮 一章, 荻野 千秋, 清水 宣明

    To well characterize disinfection system and to investigate the mechanisms of the cell killing by TiO_2/U.S. method, Escherichia coli (NOVABLUE strain) was used as a model and target microorganism. Our previous studies reported that ultrasonic irradiation was very effective to kill the microbial cells. Microbial cells are physically and chemically sterilized by ultrasonic irradiation. Our present study was under taken, to investigate a suitable mechanism for cells killing and to develop the sterilization effect using electrochemically under U.S. in the presence of TiO_2. We suggested that, toxic substances such as free OH radical generated by electrolysis have been shown to be responsible for the sterilization effect.

    日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 16 - 17, 日本語

  • ラハマン モハマド ミザヌル, 荒川 雅幸, 仁宮 一章, 荻野 千秋, 清水 宣明

    Strong bacteriocidal activity of titanium dioxide (TiO_2) irradiated with ultrasound (U.S.) is an established phenomenon. A new non-woven TiO_2 fabric under U.S. system was used to well characterize the sonocatalytic disinfection and lipid peroxidation of Escherichia coli (E. coli) cells membrane and to investigate the possible killing mechanisms of its cell. It was found that applying ultrasonic irradiation with non-woven TiO_2 fabrics reduces the E. coli cell concentration very rapidly, which indicated very high disinfection rate at this system. Diphenyl-1-pyrenylphosphin (DPPP) was used as a non-fluorescent probe for lipid peroxidation on E. coli cells membrane. Reaction of DPPP with U.S. treated samples and organic hydroperoxides were examined by monitoring increase in fluorescence intensity of DPPP oxide (DPPP=O). The mechanism of cell killing and lipid peroxidation were further investigated by examining the effect of OH radical scavenger. The scavenger studies indicated the functional role of hydroxyl (OH) radicals in the initiation of ultrasound induced lipid peroxidation.

    日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 48 - 49, 英語

  • 大庭 達哉, 仁宮 一章, 荻野 千秋, 清水 宣明

    Effects of TiO_2 particles addition on OH radical formation by ultrasonic irradiation were investigated through comparison of Al_2O_3 particles. Formation of cavitation bubbles is accelerated on the surface of particles because sonoluminescence intensity and white noise intensity are increased by particle addition. It is suggested that there is the close relationship between the effeciency of TiO_2 on OH radical formation and cavitation phenomena. Irradiation frequency is the very important factor for cavitation phenomena. In this reseach, the effects of frequency changes on OH radical formation by ultrasonic irradiation were investigated in the presence of TiO_2.

    日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 50 - 51, 日本語

  • 仁宮 一章, 園家 史朗, 大島 周平, 荻野 千秋, 黒田 俊一, 清水 宣明

    Our previous study demonstrated that the titanium dioxide (TiO_2) could catalyze production of hydroxyl (OH) radicals by ultrasound (U.S.) irradiation even without any photo-excitation. The purpose of this study was to extend the applicability of our "TiO_2/U.S. system" to drug delivery systems for cancer therapy by modifying the surface of TiO_2 nanoparticles with PreS1/S2 protein for targeting HepG2 cells. To evaluate the specific binding of the preS1/S2-conjugated TiO_2 particles (preS1/S2-TiO_2), liver and colon cancer cell lines, HepG2 and WiDr were incubated with the preS1/S2-TiO_2 (0.01% (v/v)) for 6 hours and subjected to fluorescent immunostaining with anti-preS1 antibody. The fluorescence was detected only in the case of HepG2 cells, indicating specific binding and incorporation of preS1/S2-TiO_2. To evaluate the sonocatalytic cytotoxicity of HepG2 cells with incorporation of preS1/S2-TiO_2, the culture dish was irradiated with U.S. at the frequency of 1MHz and with output power of 0.3 W/m^2 for 30 sec (50% duty). Compared to the control condition without preS1/S2-TiO_2 incorporation and/or U.S. irradiation, the growth of HepG2 cells was restrained with both the preS1/S2-TiO_2 incorporation and the U.S. irradiation at initial time of culture, suggesting our "TiO_2/U.S. system" could be promising tool for cancer therapy.

    日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 96 - 97, 日本語

  • 吉野 和慶, 伊藤 吉基, 宮下 徹, 伊藤 裕子, 清水 宣明, 荻野 千秋

    公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 380 - 380, 日本語

  • 伊藤 吉基, 伊藤 裕子, 宮下 徹, 吉野 和慶, 清水 宣明, 荻野 千秋

    公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 381 - 381, 日本語

  • 石井 純, 田中 勉, 松村 静香, 立松 健司, 黒田 俊一, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 348 - 348, 日本語

  • 崎山 和亨, 大庭 達哉, 荻野 千秋, 清水 宣明

    公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 225 - 225, 日本語

  • Chiaki Ogino, Koki Kanehira, Ryosuke Sasai, Shuji Sonezaki, Nobuaki Shimizu

    Polyelectrolyte polyacrylic acid (PAA), used in the chemical modification of titanium dioxide (TiO2) nanoparticles, allows TiO2 nanoparticles to remain in suspension at neutral pH. The anti-17 beta-estradiol (E2) antibody was immobilized on PAA-modified TiO2 (PAA-TiO2) nanoparticles via covalent bonding between the carboxylic acid of PAA and the amino group of the antibody. The anti- E2-antibody-immobilized TiO2 (E2Ab-PAA-TiO2) nanoparticles can form a suspension at neutral pH, with a particle size of less than 100 nm. The E2-PAA-TiO2 nanoparticles caused the photocatalytic degradation of a typical TiO2 substrate, methylene blue. The anti-E2 antibody immobilized on the TiO2 surface recognized and bound E2 in the solution, thereby improving the efficiency of E2 degradation compared with that of PAA-TiO2 nanoparticles. These results demonstrate that the E2Ab-PAA-TiO2 nanoparticles developed in this study can be used in water treatment technology. Furthermore, this strategy of immobilizing proteins on nanoscale TiO2 particles creates new applications not only in the treatment of environmental waste, but also in medical and public sanitation processes.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2007年10月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104 (4), 339 - 342, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiaki Ogino, Masayuki Kanemasu, Masashi Fukumoto, Takafumi Kubo, Takamichi Yoshino, Akihiko Kondo, Hideki Fukuda, Nobuaki Shimizu

    Using biomass support particles (BSPs) as a cell immobilized matrix, immobilized recombinant Streptomyces lividans continuously produced phospholipase D (PLD) in a yield of about 1.5 x 10(4) U/L in each of eight batches. In contrast to the original strain Streptoverticillium cinnamoneum, this heterologous expression system with an immobilization method is capable of producing secretory PLD with an 8-fold greater efficiency. The presence of both glucose and tryptone in the initial culture medium also promoted secretory production, and PLD activity around 3.0 x 10(4) U/L were achieved. In addition, the promoter region of PLD ORF was deduced, and three types of plasmid having different lengths of promoter sequence were constructed. The deduced sequence had same effect on either of PLD production or mycelium immobilization, and the transformants harboring each of three plasmids showed the similar cultivation profiles (3.0 x 10(4) U/L). A combination of the immobilization method with BSPs and S. lividans transformant harboring the deduced plasmid has the potential for producing secretory PLD in the culture supernatant continuously. (c) 2007 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2007年07月, ENZYME AND MICROBIAL TECHNOLOGY, 41 (1-2), 156 - 161, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiaki Ogino, Hidenori Daido, Yuka Ohmura, Namiko Takada, Yoshiki Itou, Akihiko Kondo, Hideki Fukuda, Nobuaki Shimizu

    The gene that encodes phospholipase D (PLD) from Streptoverticillium cinnamoneum contains three consensus regions (Region 1, 11 and Was shown in Fig. 1A) that are conserved among the PLD superfamily. The glycine-glycine (GG) motif in Region I and the glycine-serine (GS) motif in Region IV are also conserved in the PLD superfamily. These (GG and GS) motifs are located 7 residues downstream from each HKD motif. In an investigation of fifteen GG/GS motif mutants, generated as fusion proteins with maltose-binding protein (MBP-PLDs), three highly active mutants were identified. Three of the mutants (G215S, G216S, and G216S-S489G) contained a serine residue in the GG motif, and exhibited approximately a 9-27-fold increased transphosphatidylation activity to DPPC compared with recombinant wild type MBP-PLD. When heat stability was compared between three mutants and the recombinant wild type, only G216S-S489G showed heat labile properties. It appears that the 489th serine residue in the GS motif also contributes to the thermal stability of the enzyme. In addition, the GG/GS motif was very close to the active center residue, including two HKD motifs, as shown by computer modeling. The findings suggest that the GG/GS motif of PLD is a key motif that affects catalytic function and enzymatic stability. (c) 2007 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2007年06月, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1774 (6), 671 - 678, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Shimizu, Chiaki Ogino, Mahmoud Farshbaf Dadjour, Tomoyuki Murata

    A series of experiments were carried out to study the degradation of methylene blue by the irradiation of ultrasound onto TiO2 in aqueous solution. A statistically significant decrease in the concentration of methylene blue was observed after 60 min irradiation. While the reduction was 22% of the initial concentration without H2O2, addition of H2O2 significantly enhanced the degradation of methylene blue for the TiO2 containing system (85% reduction of the initial concentration). The addition of H2O2 had no effect on the methylene blue degradation when the system contained Al2O3. The degradation ratio of methylene blue was dependent on the amount of TiO2 and also the specific surface area of TiO2 in the solution. The effects of radical scavenging agents on the degradation of methylene blue were also investigated for the system with TiO2. It was found that the radical scavenging agents dimethyl sulfoxide (DMSO), methanol, and mannitol suppressed the degradation, with DMSO being the most effective. The effect of pH on the degradation of methylene blue was further investigated. An U-shaped change in the concentration of methylene blue in the presence of TiO2 was observed along with the change in pH values (pH 3-12), and the highest degradation ratio was observed at around pH 7. In conclusion, ultrasound irradiation of TiO2 in aqueous solution resulted in significant generation of hydroxyl radicals, and this process may have potential for the treatment of organic dyes in wastewater. (C) 2006 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2007年02月, ULTRASONICS SONOCHEMISTRY, 14 (2), 184 - 190, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rahman Mohammad Mizanur, Ogino Chiaki, Shimizu Nobuaki

    日本ソノケミストリー学会, 2007年, ソノケミストリー討論会講演論文集, 2007, 102 - 102, 英語

  • 久保 孝文, 吉野 和慶, 伊藤 吉基, 宮下 徹, 荻野 千秋, 清水 宣明

    公益社団法人 化学工学会, 2007年, 化学工学会 研究発表講演要旨集, 2007, 353 - 353, 日本語

  • Chiaki Ogino, Mahmoud Farshbaf Dadjour, Keiko Takaki, Nobuaki Shimizu

    An ultrasonic system with TiO2 particles was utilized for disinfection of Escherichia coli (E coli) under condition of darkness. The mechanism of disinfection was studied by investigating the cell lysis and degradation of DNA leaked through the cell membrane. The degradation of DNA was enhanced remarkably in the presence of TiO2 particles. The effects of Al2O3 particles on the degradation of DNA were also examined, and these particles were found to be less effective than TiO2 particles at enhancing degradation. Compared with TiO2 addition case, the ultrasonic irradiation with no particles afforded 5% degradation, while the addition of Al2O3 induced approximately 54% degradation under the conditions used. The role of hydroxyl radicals in this process was also investigated by examining the effects of a hydroxyl radical scavenger, glutathione. A significant suppression of DNA leakage through the cell membrane was observed in the solutions containing glutathione, and the suppressive effects were dose-dependent. These results indicate that hydroxyl radicals may play a primary role in the degradation of DNA as well as cell lysis. (c) 2006 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2006年11月, BIOCHEMICAL ENGINEERING JOURNAL, 32 (2), 100 - 105, 英語

    [査読有り]

    研究論文(学術雑誌)

  • MF Dadjour, C Ogino, S Matsumura, S Nakamura, N Shimizu

    An ultrasonic treatment system, using a TiO2 photocatalyst, was used to disinfect Legionella pneumophila. A kinetic study of the process indicates that TiO2 Significantly improves the disinfection process. The concentrations of viable cells were reduced to 6% of the initial concentrations in the presence of 0.2 g/ml TiO2 after a 30 min of treatment period, while only an 18% reduction was observed in the absence of TiO2. The potency of the disinfection could be enhanced, to some extent, by increasing the amount of TiO2 used. Cell concentrations were decreased by an order of 3 within 30 min of treatment in the presence of 1.0 g/ml TiO2. The disinfection power in the presence of TiO2 versus Al2O3 was also compared and the findings showed that TiO2 induced a higher cell killing. No significant effect of initial cell concentration on the disinfection was found in the range of 10(2)-10(7) CFU/ml after a 30 min of treatment period. The mechanism of cell killing was investigated by examining the effects of OH radical scavengers such as ascorbic acid, histidine and glutathione. The disinfection power was reduced in samples that contained these radical scavengers, thus indicating the importance of OH radicals. (c) 2006 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2006年03月, WATER RESEARCH, 40 (6), 1137 - 1142, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 高木 圭子, 荻野 千秋, 清水 宣明

    Titanium dioxide (TiO_2) is known as photocatalytic material, and it generate reactive oxygen species (ROS) according to be excited by ultraviolet rays (UV) under 380nm. Those ROS generated from TiO_2 could sterilize E coli, and have cytotoxic effect on mammalian cancer cells. Previously, we reported the generation of OH radicals from TiO_2 particles excited by ultrasonic (US) irradiation, not UV. Furthermore, the TiO_2 excited by US exhibited decompose activity on E coli and chemical substances. In this study, we report the cytotoxic effect of US in the presence of TiO_2 particles in cell culture media. To estimate the cytotoxic effect, we measured lactase dehydrogenase (LDH) activity leaked from damaged cell numbers after US irradiation. Immediately after US irradiation in the presence of TiO_2 particles, the LDH activity exhibited high, indicating TiO_2 with US irradiation caused damage on cell membrane stability. Furthermore, we confirmed the cell membrane damage by flowcytometry assay with Annexin V-FITC/Propidium iodide (PI) stain method, Annexin V-negative/PI positive cells were dominant immediately after US irradiation in the presence of TiO_2 particles.

    日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 1 - 2, 日本語

  • 藤平 敦司, 崎山 和亨, 荻野 千秋, 清水 宣明

    Generation of OH radicals by ultrasound irradiation in the presence of TiO_2 was studied. The effects of dissolved argon and xenon applied into the irradiating solutions were studied in the presence of TiO_2. Heat capacity ratio of argon and xenon is higher than that of nitrogen and oxygen. Higher heat capacity ratio of dissolved argon and xenon causes higher local temperature when cavitation bubble collapsed, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Further, the effect of the photocatalysis of TiO_2 may be more intensified by high temperature and sonoluminescence.

    日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 99 - 100, 日本語

  • 崎山 和亨, 藤平 敦司, 荻野 千秋, 高木 圭子, 清水 宣明

    The formation of H_2O_2 was studied with ultrasound irradiation in the presence of TiO_2 or Al_2O_3 particles. The effect of varying the temperature of cavitation bubbles in aqueous solutions of two rare gases (Ar, Xe or mixture of Xe and Ne (Xe:Ne=3:7)) on the formation of H_2O_2 were estimated. The very high temperatures (several thousand K) of collapsing gas bubbles lead to the thermal dissociation of water molecules into OH radicals, and the reaction of two OH radicals causes the formation of H_2O_2 consequently. After 60 min sonication with 25〜36kHz ultrasound the highest concentration of H_2O_2 (612μM) was observed when Xe was saturated in the solution. While the increased concentration of H_2O_2 after 60 min sonication was lower (135μM) in the solution saturated with Ar or mixture of Xe and Ne. The difference in the increased concentration of H_2O_2 was not significant between Ar and mixture of Xe and Ne. These results suggest that the threshold for the formation of cavitation bubbles seems to be the lowest in the solution saturated with Xe and to be the important factor for the formation of H_2O_2 during the sonication in the presence of TiO_2 or Al_2O_3, respectively.

    日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 87 - 88, 日本語

  • Rahman Mohammad Mizanur, 荻野 千秋, 清水 宣明

    To well characterize disinfection system and to investigate the mechanism of cell killing by TiO_2/US method, Escherichia coli (E. coli) was used as a model and target microorganism. At present, our research is going on developing disinfection system by using ultrasonic reactor in the presence of nonwoven TiO_2 fabric. It was found that the decrease in cell concentration was almost insignificant without ultrasonic irradiation although TiO_2 filter was present, and vice versa. In contrast, applying ultrasonic irradiation with TiO_2 filter reduces the E. coli cell concentration very rapidly which indicated very high disinfection power at this condition or system. However, the effect of new ultrasonic reactors on the disinfection rate, the characterization of affecting parameters of the ultrasonic reactors, as well as possible germicidal mechanism of disinfection remain to be studied. Given an opportunity to conduct these research will uncover above issues and will be benefited our scientific career to develop, in addition to enrich the bio-industrial field as well.

    日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 103 - 104, 英語

  • 宮地 祐介, 荻野 千秋, 清水 宣明

    公益社団法人 化学工学会, 2006年, 化学工学会 研究発表講演要旨集, 2006, 997 - 997, 日本語

  • 藤平 敦司, 崎山 和享, 荻野 千秋, 清水 宣明

    公益社団法人 化学工学会, 2006年, 化学工学会 研究発表講演要旨集, 2006, 783 - 783, 日本語

  • MF Dadjour, C Ogino, S Matsumura, N Shimizu

    The kinetics of disinfection of Escherichia coli, in the presence of a TiO2 photocatalyst, using an ultrasonic irradiation system was investigated. TiO2 was found to significantly improve the disinfection process. A 98% reduction in the concentrations of viable cells was obtained in the presence of TiO2 during a 30 min period of irradiation, while only a 13% reduction was observed when an ordinary ultrasonic irradiation system was used. The rate of cell killing was also higher in the presence of TiO2 compared with Al2O3. The rate of disinfection was proportional to the amount of TiO2 in the concentration range examined. Cell concentrations were decreased by an order of 5 within 10 min of irradiation in the presence of 2.0 g/ml TiO2. No significant effect of cell concentration on the cell-killing process was found in the range of 10(3) to 10(7) CFU/ml. The mechanism of cell killing was further investigated by examining the effects of OH radical scavengers, such as histidine and glutathione. The rate of disinfection was decreased in samples containing these radical scavengers, thus indicating that radicals are of major importance in this process. (c) 2005 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2005年10月, BIOCHEMICAL ENGINEERING JOURNAL, 25 (3), 243 - 248, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Improvement of ultrasonic disinfection power using TiO2 photocatalyst

    MF Dadjour, C Ogino, S Matsumura, S Nakamura, N Shimizu

    The disinfection power of an ultrasonic system was enhanced using TiO2 photocatalyst in the irradiating solutions. Cultures of Legionella were used in the irradiation system with and without TiO2. A significant decrease in the concentration of viable cells was observed during irradiation in the presence of TiO2. The rate of cell killing was higher in the presence of TiO2 than it was with Al2O3, and was proportional to the amount of TiO2 used in the irradiating samples. There was no significant effect of cell concentration on the rate of cell killing in the range of 10(3) to 10(7) CFU/ml. Addition of OH radical scavengers such as glutathione, ascorbic acid and histidine to the irradiating solutions reduced the rate of disinfection, thus indicating the primary role of OH radicals in this process.

    AMER INST PHYSICS, 2005年, 4th International Symposium on Therapeutic Ultrasound, 754, 275 - 277, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Dadjour Mahmoud Farshbaf, 荻野 千秋, Matsumura Susumu, 清水 宣明

    日本ソノケミストリー学会, 2005年, ソノケミストリー討論会講演論文集, 14, 13 - 14, 英語

  • 藤平 敦司, 荻野 千秋, Dadjour Mahmoud Farshbaf, 清水 宣明

    Generation of OH radicals by ultrasonic irradiation in the presence of TiO_2 was studied. OH radicals are oxidizing agents and effective for the degradation of hazardous organic compounds. However, the mechanisms of OH radicals formation have not been elucidated yet. In this work, the effects of cavitation induced by ultrasonic irradiation in water was considered and investigated. As the lifetime of OH radical is very short and can be hardly analyzed directly, then sodium salicylate was used as OH radicals trapping agent. The addition reaction of OH radicals and salicylate results in the formation of dihydroxy benzoic acid(DHBA). The generation of DHBA by ultrasonic irradiation in the presence of TiO_2 as an indicator of OH radicals was investigated in complete darkness. The effects of particles (TiO_2 and A1_2O_3) on the generation of DHBA were examined(Fig.2). While no generation of DHBA was detected in the solutions without particles, higher DHBA concentration was obtained in the presence of TiO_2 than that of Al_2O_3. This enhancement in the formation of OH radicals may be due to the pyrolysis of H_2O and the photocatalysis of TiO_2 with sonoluminessence. Sonoluminessence may be due to cavitation, and the exsitence of particles couse more cavitation whuch may increase sonoluminessence effect. [graph] Also, the effects of dissolved argon applied into the irradiating solutions were studied in the presence of TiO_2 and Al_2O_2 (Fig.5, 6). Higher amount of DHBA was obtained in the solutions in the presence of TiO_2. Thermal capacity of argon is higher than that of nitrogen and oxygen. Higher thermal capacity of dissolved argon causes higher local temperature during cavitation, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Moreover, the effect of the photocatalysis of TiO_2 may be more intensified in the presence of dissolved argon. [graph] [graph] Consequently, the advantage of TiO_2 in the ultrasonic system for the formation of OH radicals was shown in this research.

    日本ソノケミストリー学会, 2005年, ソノケミストリー討論会講演論文集, 14, 91 - 93, 日本語

  • 福本 将士, 荻野 千秋, 清水 宣明

    公益社団法人 化学工学会, 2005年, 化学工学会 研究発表講演要旨集, 2005, 287 - 287, 日本語

  • 泉 麻里子, 荻野 千秋, 清水 宣明

    公益社団法人 化学工学会, 2005年, 化学工学会 研究発表講演要旨集, 2005, 280 - 280, 日本語

  • Over-expression system of phospholipase D from actinomycete by Streptomyces lividans

    OGINO Chiaki, KANEMASU M, HAYASHI Y, KURODA S, KONDO Akihiko, SHMIZU N, TANIZAWA K, FUKUDA Hideki

    2004年06月, Applied Microbiology and Biotechnology, Vol 64, pp. 823-828, 英語

    [査読有り]

    研究論文(学術雑誌)

  • NMR Talukder, MM Zaman, Y Hayashi, JC Wu, T Kawanishi, C Ogino, N Shimizu

    The addition of short chain polyethylene glycols (PEGs) activated Chromobacterium viscosum lipase in AOT reverse micelles. In this study, it was assumed that when AOT reverse micelles contained PEG molecules, native and activated lipases contributed to the reaction according to their weight fraction in the system. The fractions of individual lipases and their specific activities were estimated by using kinetics parameters determined by previous kinetic models. The maximum specific activity of PEG-activated lipase appeared to be 2.7 times higher than that of native lipase. The predicted weighted-average activity agreed well with experimental data. The results showed that the previous kinetic models could be used to estimate the fraction of lipase activated by PEG molecules and their specific activity. (C) 2004 Society of Chemical Industry.

    JOHN WILEY & SONS LTD, 2004年03月, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 79 (3), 273 - 276, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Over-expression system for secretory phospholipase D by Streptomyces livedanse

    OGINO Chiaki, KANEMASU Masayuki, HAYASI Yasuhiro, KONDO AKIHIKO, SHIMIZU Noriaki, TOKUYAMA Shinji, TAHARA Y, KURODA Syunicni, TANIZAWA Katsyuyuki, FUKUDA Hideki

    2004年, Applied and Microbiogy and Biotechnology, 64,823-828, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 松村 享, Farshbaf D.M., 吉川 文恵, 荻野 千秋, 清水 宣明

    Introduction Titanium dioxide in the rutile-pellet form was used as a catalyst in the ultrasonic irradiation system to generate hydroxyl radicals in order to disinfect the water. Cultivated culture of Legionella was used for the kinetically investigation of cell destruction and its mechanism. The formation of active species such as hydroxyl radical, hydrogen peroxide, superoxide and etc can be promoted during ultrasonic irradiation in the presence of TiO_2. It may be due to the concentrated energy of the exploded bubbles on the surfaces of titanium granules caused during acoustic cavitations. These reactive oxygen species (ROS) may disrupt or damage various cell or viral functions and structures. Improvement of this novel disinfection method and studying the mechanism of this system were the purposes of this investigation. Materials and methods -Preparation of Cultures Legionella pneumophila(GIFU 9888) was used in this research as the model microorganisms. Legionella were incubated on BCYE plate and transferred to BYE medium. The inoculated culture was incubated in a shaker incubator at 110 rpm for 48 hrs at 37℃. Then, the culture broth was diluted to a suitable concentration. Ten ml of the diluted broth was transferred to the bottles containing TiO_2 pellets at different concentrations. -Ultrasonic irradiation Bottles were irradiated at 36 KHz, 300 W and samples were driven at different time courses. The temperature was kept constant at 20℃ during irradiation. The irradiated samples were diluted to suitable concentrations and plated on BCYE plates. The plates were incubated for 72 hrs at 37℃. The number of colonies on the plates was counted after incubation. Results and Discussion Ultrasonic irradiation of diluted culture for 15 minutes showed 92% reduction in the number of viable cell concentration at the presence of TiO_2. The rate of disinfection was compared in the presence of TiO_2 and Al_2O_3. TiO_2 induced higher rate of cell killing compared with Al_2O_3. Also, investigating the effect of TiO_2 concentration indicated that the rate of disinfection was proportional to the concentration of TiO_2. The mechanism of cell killing was further investigated by examining the effects of OH radical scavengers such as ascorbic acid and glutathione. Lower rates of disinfection were observed in the samples containing these radical scavengers, thus indicating the primary role of OH radicals in this process.

    日本ソノケミストリー学会, 2004年, ソノケミストリー討論会講演論文集, 13, 38 - 40, 日本語

  • 舩越 正人, Farshbaf D., 吉川 文恵, 荻野 千秋, 清水 宣明

    Irradiation of TiO_2 particles under ultraviolet light has been known to be a source of OH radicals in the disinfection or oxidizing systems. However, a new system was devised in this study for production of OH radicals by replacing UV with ultrasonic system (US). Formation of OH radicals was investigated in a TiO_2/US system to study the mechanism of formation of OH radicals as well as enhancement of cavitation effect in the ultrasonic system. Formation of OH radicals in the irradiating solution was studied by the reaction of OH radicals with salicylate. 2,3-dihydroxybenzoicacid (2,3-DHBA) and 2,5-dihydroxybenzoicacid (2,5-DHBA) can be formed during this reaction and they can be measured by HPLC with an electro chemical detector (HPLC-ECD; Eicom). Formation of DHBAs could be detected during the irradiation of salicylate solution under ultrasonic system and in the presence of TiO_2. It was seen that the concentration of DHBAs was increased within 20 minutes irradiation and then decreased in spite of high concentration of salicylate. It was likely due to the oxidation and degradation of DHBA in the ultrasonic system. Therefore, for investigating the oxidation of DHBA in the presence of salicylate, more salicylate was added after 20 minutes reaction to the irradiating mixture consecutively. The concentration of DHBA was increased for three times by the addition of salicylate concentration in the solution. Enhancement of cavitation energy was also investigated by the addition of various gases (Ar, O2, N2) into irradiation solution. The ratio of the specific heats at constant pressure and constant volume (Cp/Cv) of the gas and vapor inside the cavity is known to be one of the most crucial parameters that determine the final temperature inside cavitation bubbles. Monoatomic gases, such as argon has a high value of Cp/Cv, while diatomic gases, such as N_2, O_2 have a lower values. Therefore, addition of argon to the irradiating solution induced higher concentration of OH radicals due to higher cavitation energy. Our results indicated that the addition of O_2 and N_2 gases at the same conditions resulted lower oxidation power. Finally, the competitive oxidation of salicylate and DHBAs in the irradiating solutions was more investigated in this research to develop a more descriptive mechanism of generation of hydroxyl radicals.

    日本ソノケミストリー学会, 2004年, ソノケミストリー討論会講演論文集, 13, 94 - 96, 日本語

  • S Ikeno, C Ogino, T Ito, Y Sugino, N Shimizu

    TOL plasmid pWW0 of Pseudomonas putida mt-2 encodes a series of enzymes for the degradation of benzene derivatives. In the presence of benzene derivatives, the complex formed from the regulating protein XylR and Ps promoter controls the expression of these enzymes in P. putida. For detecting of benzene derivatives, the plasmid called as pTS301-GFP has been constructed by introducing a gene encoding green fluorescent protein (GFP) into the downstream of the Ps promoter gene. GFP functioned as a reporter protein is transcribed by activation of the XylR complex. In the present study, the effects of the composition of culture medium and ATP addition on fluorescence of transformant were investigated. The optimal culture medium was found to NZM, and presence of casamino acid decreases the sensitivity for detection of chemicals. Furthermore, it was observed that the addition of 200 muM ATP was effective for detecting of fluorescence of transformants exposed to the low concentration (0.05 mM) of benzene derivatives for 4 h. Applying these results for the detection of the benzene derivatives, it will be expected that transformant with pTS301-GFP can be useful for environmental water evaluation. (C) 2003 Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2003年12月, BIOCHEMICAL ENGINEERING JOURNAL, 16 (3), 273 - 278, 英語

    [査読有り]

    研究論文(学術雑誌)

  • C Ogino, S Kuroda, S Tokuyama, A Kondo, N Shimizu, K Tanizawa, H Fukuda

    This review is focusing on an industrially important enzyme, phospholipase D (PLD), exhibiting both transphosphatidylation and hydrolytic activities for various phospholipids. The transphosphatidylation activity of PLD is particularly useful for converting phosphatidylcholine (PC) into other phospholipids. During the last decade, the genes coding for PLD have been identified from various species including mammals, plants, yeast, and bacteria. However, detailed basic and applied enzymological studies on PLD have been hampered by the low productivity in these organisms. Efficient production of a recombinant PLD has also been unsuccessful so far. We recently isolated and characterized the PLD gene from Streptoverticillium cinnamoneum, producing a secretory PLD. Furthermore, we constructed an overexpression system for the secretory enzyme in an active and soluble form using Streptomyces lividans as a host for transformation of the PLD gene. The Stv. cinnamoneum PLD was proven to be useful for the continuous and efficient production of phosphatidylethanolamine (PE) from phosphatidylcholine. Thus, the secretory PLD is a promising catalyst for synthesizing new phospholipids possessing various polar head groups that show versatile physiological functions and may be utilized in food and pharmaceutical industries. (C) 2003 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2003年09月, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 23 (2-6), 107 - 115, 英語

    [査読有り]

    研究論文(学術雑誌)

  • S Ikeno, C Ogino, T Ito, N Shimizu

    The TOL plasmid of Pseudomonas putida mt-2 encodes a series of enzymes for the degradation of benzene derivatives. The expression of the enzymes is controlled by the regulating protein XylR, of which the promoter, known as Ps, is activated in the presence of benzene derivatives. Sensitive and convenient sensing of benzene derivatives has been developed by fusing a gene encoding a green fluorescent protein (GFP) into the downstream of the Ps promoter. In the plasmid pTS301-GFP, GFP as a reporter protein is transcribed by controlling the promoter's activation by Xy1R and benzene derivatives. In the present study, a recombinant Escherichia coli transformed with this plasmid was applied to the environmental sensing of benzene derivatives in water. The expression of GFP was confirmed in water containing 0.1 mM m-xylene and fluorescence intensity was quantified by microplate reader. The optimal temperature for GFP expression was 27 degreesC and the lowest detectable concentration of xylene derivatives 0.1 mM. Benzene derivatives having CH3- or Cl- or both in the side chain of the benzene ring showed particularly high fluorescence intensity. To conclude, transformants harboring pTS301-GFP constitute an adaptable system for easy sensing of small amounts of benzene derivative in water. (C) 2003 Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2003年09月, BIOCHEMICAL ENGINEERING JOURNAL, 15 (3), 193 - 197, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/isooctane reverse micellar system

    MMR Talukder, T Takeyama, Y Hayashi, JC Wu, T Kawanishi, N Shimizu, C Ogino

    The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-1-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse micellar system, the phase diagram of AOT/ PEG 400/water/isooctane system was studied. The influences of relevant parameters on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable, retaining >75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics parameter maximum velocity (V-max) exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by the addition of PEG 400.

    HUMANA PRESS INC, 2003年08月, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 110 (2), 101 - 112, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Structural investigation of water trapped in AOT/isooctane reverse micelles containing PEG by Fourier transform infrared spectroscopy

    T Takeyama, Y Hayashi, M Talukder, N Shimizu, T Kawanishi, C Ogino

    The structure of water entrapped in AOT/PEG 400 reverse micelles was investigated and compared with that of simple AOT reverse micelles by using Fourier transform infrared spectroscopy (FT-IR) to measure the stretching vibrations for O-H, carbonyl (C=O) and SO3- (S=O) bonds. The water in a reverse micelle consists of trapped water, bound water and free water. The fraction of free water in the water pool of reverse micelles containing PEG increases with the molar ratio of AOT to H2O, W-o more steeply than that of simple reverse micelles, and the structure of bound water is disrupted by the addition of PEG. The shift of the peak of the S = O bond and analysis of the C = O bond spectra indicates that the interaction of AOT molecules with water is decreased by hydrated PEG molecules at W-o > 6, which coincides with the results of O-H spectra.

    SOC CHEMICAL ENG JAPAN, 2003年01月, KAGAKU KOGAKU RONBUNSHU, 29 (1), 124 - 130, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Farshbaf D.M., 清水 宣明, 松村 享, 荻野 千秋

    日本ソノケミストリー学会, 2003年, ソノケミストリー討論会講演論文集, 12, 30 - 32, 日本語

  • Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum

    C Ogino, Y Yasuda, A Kondo, N Shimizu, H Fukuda

    A new reaction model for transphosphatidylation and hydrolysis reactions by PLD has been constructed and simulated under various experimental conditions. The reaction model is based on the Ping-Pong mechanism and composed of PLD-phosphatidyl complex intermediates. In order to accommodate the inhibition of the initial reaction rate at high nucleophile concentration, a phosphatidyl-enzyme complex associating with two nucleophile molecules [P-PLD-X-2] is introduced into the transphosphatidylation scheme of the proposed reaction model. In both cases (phosphatidylethanolamine (PE) production, and phosphatidylglycerol (PG) production from phosphatidylcholine (PC)), the simulation results agreed well with the experimental data. While the PE production rate at the initial state was inhibited at high concentrations of the nucleophile (ethanolamine), the PG production rate did not show inhibition. Combination of the PE and PG production schemes is expected to make multi-phospholipid production from PC possible, thus, making this model of potential use for phospholipid synthesis in engineering applications. (C) 2002 Elsevier Science B.V All rights reserved.

    ELSEVIER SCIENCE SA, 2002年03月, BIOCHEMICAL ENGINEERING JOURNAL, 10 (2), 115 - 121, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Shimizu, Chiaki Ogino, Takuya Kawanishi, Yoshishige Hayashi

    To quantify individual behavioral responses to toxic chemicals, the swimming motion of individual Daphnia magna was continuously monitored using a motion analysis system. The fractal dimension was introduced to compare the straightness or complexity of the swimming trajectory before and after exposure to toxic chemicals. Analysis indicated that the swimming trajectory of individual Daphnia has a fractal structure. The basal fractal dimension in the control medium was 1.35±0.01 (n = 50 Daphnia). Exposure to CuSO4, (10 μg/L), organophosphorus (Dichlorvos 10 μg/L), and carbamate (Propoxur 500 μg/L) pesticide caused a significant increase in the fractal dimension with a latency of 60 min, reaching a maximal level of 2.26±0.34, 2.43±0.19, and 2.51±0.21, respectively, after a 120-min exposure. The magnitude of the change in the fractal dimension was related to the toxic chemical concentration and the exposure time. Threshold concentrations determined at 60 min of exposure were 10 μg/L for CuSO4, 5 μg/L for Dichlorvos, and 500 μg/L for Propoxur. The toxicity index (EC50) values after 120 min of exposure were 6.31 μg/L, 7.64 μg/L, and 466 μg/L for CuSO4, Dichlorvos, and Propoxur, respectively. Thus, the fractal dimension seems useful for analyzing and comparing complex trails, such as swimming trajectories, which could be used as the endpoint for an acute bioassay. © 2002 Wiley Periodicals, Inc.

    2002年, Environmental Toxicology, 17 (5), 441 - 448, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 清水 宣明, 荻野 千秋, 林 良茂, 三好 憲雄

    Heterogeneous photocatalysis over TiO_2 or ultrasonic irradiation have been reported to be a promising application for decomposition of the hazardous chemical substances. We observed the generation of ・OH in the aqueous solution during the irradiation of ultrasound on TiO_2. The purpose of this study was to verify the generation of ・OH by indicating (1) decomposition of methylene blue, (2) nucleophilic addition of ・OH to salicylic acid, which produce dihydroxybenzoic acid. The decomposition of bisphenol A (BPA) and pentachlorophenol (PCP) by the irradiation of ultrasound in presence of TiO_2 were further studied. The results are summarized as follows. Decomposition of methylene blue Experimentally it was observed that although the Al_2O_3 had no effect on the decomposition of methylene blue by ultrasonic irradiation, it was decomposed slightly by the presence of TiO_2. In addition H_2O_2 accelerated the decomposition of methylene blue for the TiO_2 containing system. But the addition of H_2O_2 had no effect on the methylene blue decomposition when the system containing Al_2O_3 only. The effect of radical scavengers on the decomposition of methylene blue was also investigated for the system with TiO_2. It was found that the scavengers such as methanol, DMSO, mannitol reduced the decomposition. Among them DMSO was the most significant. Effect of TiO_2 on the nucleophilic addition of ・OH to salicylic acid Ultrasonification of 10 μM salicylic acid (2 ml) was carried out in absence and presence of 0.4 g TiO_2 for exactly 10 minutes, and produced dihydroxybenzoic acid (DHBA) was measured by HPLC/ECD. In presence of TiO_2, significant amount of DHBA (34 pg/20μl) was produced, but almost no DHBA (2.2 pg/20μl) was found to produce without TiO_2. The effect of scavengers on the generation of DHBA was the same as it was observed in the case of methylene blue decomposition. This result inferred that the ultrasonic irradiation generated the ・OH, which is responsible for the hydroxylation of salicylic acid. Decomposition of BPA and PCP Ultrasonification of BPA and PCP, a representative endocrine disruptors, were carried out in the presence of TiO_2 (0.4 g) and the change in the concentration of BPA and PCP were measured by HPLC/ECD. The initial concentration of BPA (1 ppm) was gradually decreased with the irradiation time and was totally decomposed by 15 minute irradiation of ultrasound. The similar results were observed for PCP. It was concluded that the irradiation of ultrasound on TiO_2 in aqueous solution significantly generate hydroxyl radicals which is responsible for the decomposition of the endocrine disruptors such as BPA and PCP.

    日本ソノケミストリー学会, 2002年, ソノケミストリー討論会講演論文集, 11, 91 - 93, 日本語

  • Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity

    C Ogino, Y Negi, H Daido, M Kanemasu, A Kondo, S Kuroda, K Tanizawa, N Shimizu, H Fukuda

    A membrane-bound phospholipase D (PLD) has been identified and isolated in a soluble form from an actinomycete, Streptoverticillium cinnamoneum. The enzyme has a monomeric structure with a molecular size of about 37 kDa, bring the smallest among the enzymes so far reported. The enzyme catalyzes the hydrolysis of phosphatidylethanolamine and phosphatidylserine as preferred substrates, but not the transphosphatidylation reaction of their phospholipid groups to ethanol. Together with the absence of immunochemical cross-reactivity, these enzymatic properties demonstrate that the membrane-bound enzyme is distinct from the extracellular enzyme recently characterized and cloned from the same bacterial strain [C. Ogino ct al., J. Biochem. 125 (1999) 263-269] and is therefore regarded as a novel prokaryotic PLD. (C) 2001 Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2001年01月, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1530 (1), 23 - 31, 英語

    [査読有り]

    研究論文(学術雑誌)

  • The 5-HT1A receptor agonist, 8-OH-DPAT, attenuates stress-induced anorexia in conjunction with the suppression of hypothalamic serotonin release in rats

    N Shimizu, T Hori, C Ogino, T Kawanishi, Y Hayashi

    The effect of the selective 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) on stress-induced anorexia and serotonin (5-HT) release in the rat hypothalamus was studied with brain microdialysis. Subcutaneous injection of 8-OH-DPAT (1 mg/kg) significantly attenuated the immobilization-induced anorexia for 3 h, but had no effect during the following 9 h. Injection of 8-OH-DPAT itself had no effect on basal release of 5-HT, while it significantly blocked the immobilization-induced 5-HT release in the lateral hypothalamus. The results suggest that 8-OH-DPAT attenuated the stress-induced anorexia through the activation of 5-HT1A autoreceptors in dorsal raphe nucleus. (C) 2000 Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2000年12月, BRAIN RESEARCH, 887 (1), 178 - 182, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum

    C Ogino, Y Negi, T Matsumiya, K Nakaoka, A Kondo, S Kuroda, S Tokuyama, U Kikkawa, T Yamane, H Fukuda

    Phospholipase D (PLD), secreted into the culture medium of an actinomycete, Streptoverticillium cinnamoneum, has been purified to homogeneity and characterized. The Stv. cinnamoneum PLD efficiently catalyzes both the hydrolysis and transphosphatidylation of various phospholipids, including phosphatidylethanolamine (PE), phosphatidylcholine (PC), and phosphatidylserine (PS), However, the substrate specificity differs between the two reactions; PE serves as the most preferred substrate for the hydrolysis, but PC and PS are better substrates than PE for the transphosphatidylation. In addition, the transphosphatidylation but not the hydrolysis of PE and PC is markedly activated on the addition of metal ions, especially Al3+. Nucleotide and amino acid sequence determination of the Sty. cinnamoneum PLD revealed the presence of common structural motifs identified in all PLD sequences from various species.

    OXFORD UNIV PRESS, 1999年02月, JOURNAL OF BIOCHEMISTRY, 125 (2), 263 - 269, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kiyoshi Shimooku, Toshihiro Akisue, Hitoshi Jinnai, Tomohiro Hitomi, Chiaki Ogino, Kimihisa Yoshida, Shun-Ichi Nakamura, Yasutomi Nishizuka

    For activation of kidney membrane phospholipase D (PLD), cytosol isabsolutely needed in addition to GTP-γ-S. The active component of cytosol consists of three protein factors: ADP-ribosylation factor, RhoA, and a soluble 36-kDa protein. Any combination of these two factors synergistically activates PLD to some extent, but the presence of the three factors causes full activation. The 36-kDa protein is stable at 60°C but inactivated at 80°C for 10 min. Tissue distribution of the 36-kDa protein roughly coincides with that of PLD, suggesting physiological relevance of the protein in the regulation of PLD.

    Elsevier B.V., 1996年06月03日, FEBS Letters, 387 (2-3), 141 - 144, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shun-Ichi Nakamura, Yoshimoto Kiyohara, Hitoshi Jinnai, Tomohiro Hitomi, Chiaki Ogino, Kimihisa Yoshida, Yasutomi Nishizuka

    Bovine kidney phospholipase D (PLD) was assayed by measuring the formation of phosphatidylethanol from added radioactive phosphatidylcholine (PtdCho) in the presence of ethanol, guanosine 5'-[γ-thio]triphosphate, ammonium sulfate, and cytosol factor that contained small GTP-binding regulatory proteins. The PLD enzyme associated with particulate fractions was solubilized by deoxycholate and partially purified by chromatography on a heparin-Sepharose column. This PLD preferentially used PtdCho as substrate. After purification, the enzyme per se showed little or practically no activity but required an additional factor for the enzymatic reaction. This factor was extracted with chloroform/methanol directly from particulate fractions of various tissues, including kidney, liver, and brain, and identified as phosphatidylethanolamine (PtdEtn), although this phospholipid did not serve as a good substrate. Plasmalogen-rich PtdEtn, dioleoyl-PtdEtn, and L-α-palmitoyl-β-linoleoyl PtdEtn were effective, but dipalmitoyl- PtdEtn was inert. Sphingomyelin was 30% as active as PtdEtn. The results suggest that mammalian PLD reacts nearly selectively with PtdCho in the form of mixed micelles or membranes with other phospholipids, especially PtdEtn.

    1996年04月30日, Proceedings of the National Academy of Sciences of the United States of America, 93 (9), 4300 - 4304, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shun-Ichi Nakamura, Kiyoshi Shimooku, Toshihiro Akisue, Hitoshi Jinnai, Tomohiro Hitomi, Yoshimoto Kiyohara, Chiaki Ogino, Kimihisa Yoshida, Yasutomi Nishizuka

    Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[γ-thio]triphosphate and a cytosol fraction that contained ADP-ribosylation factor. When assayed by measuring the phosphatidyl transfer reaction to ethanol with exogenously added radioactive phosphatidylcholine as substrate, the PLD required a high concentration (1.6 M) of ammonium sulfate to exhibit high enzymatic activity. Other salts examined were far less effective or practically inactive, and this dramatic action of ammonium sulfate is not simply due to such high ionic strength. Addition of ATP but not of nonhydrolyzable ATP analogue adenosine 5'-[β,γ- imido]diphosphate further enhanced the PLD activation ≃2- to 3-fold. This enhancement by ATP needed cytosol, implying a role of protein phosphorylation. A survey of PLD activity in rat tissues revealed that, unlike in previous observations reported thus far, PLD was most abundant in membrane fractions of kidney, spleen, and liver in this order, and the enzymatic activity in brain and lung was low.

    1995年12月19日, Proceedings of the National Academy of Sciences of the United States of America, 92 (26), 12319 - 12322, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Prihardi Kahar, Akiho Itomi, Hikari Tsuboi, Miki Ishizaki, Misa Yasuda, Chie Kihira, Hiromi Otsuka, Nurlina binti Azmi, Hana Matsumoto, Chiaki Ogino, Akihiko Kondo

    Elsevier BV, 2022年07月, Metabolic Engineering, 72, 82 - 96, 英語

    研究論文(学術雑誌)

  • Nova Rachmadona, Yusuke Harada, Jerome Amoah, Emmanuel Quayson, Martha Aznury, Shinji Hama, Akihiko Kondo, Chiaki Ogino

    Elsevier BV, 2022年06月, Journal of Environmental Chemical Engineering, 10 (3), 107550 - 107550, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • 好冷性海洋細菌が産生するβ-1,3-キシラナーゼの機能解析

    谷口瑞季, 工藤基徳, 荻野千秋, 近藤昭彦, 田丸浩, 青木恭彦, 岡崎文美

    2020年, 日本農芸化学会中部支部例会講演要旨集(Web), 187th

  • 好冷性海洋細菌Psychroflexus torquis由来β-1,3-キシラナーゼの酵素化学的諸性質

    谷口瑞季, 工藤基徳, 荻野千秋, 近藤昭彦, 田丸浩, 青木恭彦, 岡崎文美

    2019年, 日本生物工学会大会講演要旨集, 71st

  • 機械学習を用いた酵素のEC number予測

    渡邉直暉, 村田昌浩, 荻野千秋, 近藤昭彦, 荒木通啓, 荒木通啓

    2018年08月07日, 日本生物工学会大会講演要旨集, 70th, 191, 日本語

  • 過酸化チタンナノ粒子の併用による放射線増感治療法の開発

    西村 勇哉, 森田 健太, 荻野 千秋, 犬伏 祥子, 佐々木 良平, 近藤 昭彦

    日本DDS学会, 2018年05月, 日本DDS学会学術集会プログラム予稿集, 34回, 204 - 204, 日本語

  • バイオマスのイオン液体前処理と酵素糖化により得られる残渣リグニンのアルカリ酸化分解特性

    江口真央, 仁宮一章, 落合芋葉子, 黒田浩介, 柘植陽太, 荻野千秋, 當摩哲也, 高橋憲司

    2018年03月05日, 日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 68th, ROMBUNNO.Z14‐02‐0930, 日本語

  • 疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発

    寺村浩, 佐々木建吾, 白井智量, 川口秀夫, 荻野千秋, 菊地淳, 佐塚隆志, 近藤昭彦, 近藤昭彦

    2016年10月14日, リグニン討論会講演集, 61st, 180‐181, 日本語

  • 凝集性酵母における新規な阻害剤耐性機構の解明

    糸見明穂, 紀平和枝, KAHAR Prihardi, 荻野千秋, 近藤昭彦

    2016年08月25日, 日本生物工学会大会講演要旨集, 68th, 316, 日本語

  • Yeast breeding for fuels and chemicals production from Indonesia Culture Collection

    KAHAR Prihardi, OTSUKA Hiromi, KIHARA Chie, ITOMI Akiho, LEE Jaemin, THONTOWI Ahmad, OCTAVIANA Senlio, JALU Filemon, KANTI Atit, YOPI, OGINO Chiaki, PRASETYA Bambang, KONDO Akihiko

    2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N316, 英語

  • Economic Value of Current Main Use of Oil Palm Empty Fruit Bunches (EFB) and Its Potential Use for Biofuel

    SIMAMORA Manaek, OGINO Chiaki, SUNARYA Yopi, KAHAR Prihardi, PRASETYA Bambang, PRASETYA Bambang, SUBIYANTO Bambang, YAMAN Aris, MAULANA Syafrizal, MALUDIN Syafrizal, MUNAWAR Sasa Sofyan

    2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N321, 英語

  • Biorefinery research in Kobe and Indonesia

    OGINO Chiaki, LEE JaeMin, KAHAR Prihardi, KONDO Akihiko

    2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N302, 英語

  • Biorefinery as strategic approach for supporting of utilization of tropical lignocellulosic biomas

    PRASETYA Bambang, SUNARYA Yopi, HERMIATI Euis, THONTOWI Ahmad, HANAFI, SIMAMORA Manaek, OGINO Chiaki, KAHAR Prihardi

    2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N313, 英語

  • マンナンバイオマスからのエタノール生産:β‐マンナナーゼとβ‐マンノシダーゼを細胞表層に提示した出芽酵母の開発

    石井純, 岡崎文美, DJOHAN Apridah Cameliawati, 原清敬, 浅井菜々実, ANDRIANI Ade, 寺村浩, KAHAR Prihardi, YOPI, PRASETYA Bambang, 荻野千秋, 近藤昭彦

    2016年03月05日, 日本農芸化学会大会講演要旨集(Web), 2016, 4A022 (WEB ONLY), 日本語

  • アルコールの添加が希硫酸前処理の効率に与える影響の解析

    寺村浩, 佐々木建吾, 白井智量, 川口秀夫, 荻野千秋, 菊地淳, 佐塚隆志, 近藤昭彦

    2016年03月05日, 日本農芸化学会大会講演要旨集(Web), 2016, 3A030 (WEB ONLY), 日本語

  • 紙パルプの同時糖化発酵によるカフェ酸の微生物生産

    川口秀夫, 勝山陽平, DU Danyao, 鶴田祥子, 南博道, 荻野千秋, 大西康夫, 近藤昭彦

    2016年, 日本農芸化学会大会講演要旨集(Web), 2016

  • 2次元NMRを用いた希硫酸前処理後固体画分の組成解析

    寺村浩, 佐々木建吾, 藍川晋平, 松田史生, 岡本真美, 白井智量, 川口秀夫, 荻野千秋, 山崎将紀, 菊地淳, 近藤昭彦

    2015年03月09日, 日本植物生理学会年会要旨集, 56th, 284, 日本語

  • 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

    KAHAR Prihardi, LEE Jaemin, 荻野千秋, 近藤昭彦

    2015年03月05日, 日本農芸化学会大会講演要旨集(Web), 2015, 3B33A01 (WEB ONLY), 日本語

  • 過酸化チタンナノ粒子による放射線増感の検討

    中山 雅央, 佐々木 良平, 西村 英輝, 吉田 賢史, 宮脇 大輔, 赤坂 浩亮, 椋本 成俊, 村岡 修, 原田 文, ノル・シャズリナ, 上原 和之, 田中 勉, 荻野 千秋, 近藤 昭彦

    (公社)日本医学放射線学会, 2014年02月, Japanese Journal of Radiology, 32 (Suppl.), 37 - 37, 日本語

  • 3P-045 バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響(オミクス解析,一般講演)

    川口 秀夫, 寺村 浩, 中村 聡子, 荻野 千秋, 原 清敬, 蓮沼 誠久, 老沼 研一, 高谷 直樹, 平野 恒, 佐塚 隆志, 北野 英己, 近藤 昭彦

    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 206 - 206, 日本語

  • 3P-226 pH 応答性ペプチドGALAを表層提示したバイオナノカプセルのエンドソーム脱出(生物化学工学,一般講演)

    西村 勇哉, 江澤 僚将, 石井 純, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 251 - 251, 日本語

  • 1P-219 稲わら前処理液の膜プロセスによるエタノール発酵高効率化(バイオマス,資源,エネルギー工学,一般講演)

    佐々木 建吾, 柘植 陽太, 佐々木 大介, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 72 - 72, 日本語

  • 1P-044 麹菌を用いたデンプンからのD- 乳酸の同時糖化発酵(遺伝子工学,一般講演)

    若井 暁, 浅井 菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 28 - 28, 日本語

  • 1P-112 放線菌を用いた糖を原料とするパラアミノ安息香酸の生産(発酵生理学,発酵工学,一般講演)

    岡井 直子, 佐藤 嘉弘, 大野 摩耶, 竹嶋 康誠, 増田 敬哉, 宮本 正教, 樋田 幸三, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 45 - 45, 日本語

  • 合成代謝経路と細胞表層工学を利用した組換え大腸菌によるセロビオースからのイソプロパノール生産(生物工学論文賞)

    相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 岡本 正宏, 花井 泰三

    日本生物工学会, 2014年, 生物工学会誌 : seibutsu-kogaku kaishi, 92 (2), 71 - 71, 日本語

  • イオン液体前処理バイオマスを用いたエタノール同時糖化発酵 (特集 イオン液体応用技術の新展開)

    荻野 千秋, 山田 亮祐, 近藤 昭彦

    小峰工業出版, 2013年02月, 化學工業, 64 (2), 136 - 139, 日本語

  • 1P-118 放線菌による芳香族化合物ホモゲンチジン酸の生産(一般講演(発酵生理学,発酵工学))

    中村 聡子, 川口 秀夫, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 47 - 47, 日本語

  • 1P-105 同時糖化発酵によるセルロースからのフェニル乳酸生産(一般講演(発酵生理学,発酵工学))

    川口 秀夫, 中村 聡子, 荻野 千秋, 老沼 研一, 高谷 直樹, 近藤 昭彦

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 44 - 44, 日本語

  • 1P-117 コリネ型細菌を用いたプロトカテク酸の生産(一般講演(発酵生理学,発酵工学))

    岡井 直子, 竹嶋 康誠, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 47 - 47, 日本語

  • 1P-183 低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵(一般講演(バイオマス,資源,エネルギー工学))

    表 小百合, 仁宮 一章, 曽田 裕司, 荻野 千秋, 高橋 憲司, 清水 宣明

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 63 - 63, 日本語

  • 1P-218 バイオマス分解性向上を目的とする酸導入イオン液体を用いた新規前処理法開発(一般講演(バイオマス,資源,エネルギー工学))

    小倉 一真, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 72 - 72, 日本語

  • 2P-149 低コストイオン液体によるエタノール製造プロセスの提案(バイオマス,資源,エネルギー工学,一般講演)

    齋藤 聡志, 石田 亘広, 神谷 典穂, 荻野 千秋

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 141 - 141, 日本語

  • 2P-148 稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮(バイオマス,資源,エネルギー工学,一般講演)

    佐々木 建吾, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 141 - 141, 日本語

  • 3P-174 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発(生物化学工学,一般講演)

    江澤 僚将, 西村 勇哉, 石井 純, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 231 - 231, 日本語

  • Shuhei Noda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    ELSEVIER SCIENCE BV, 2012年09月, NEW BIOTECHNOLOGY, 29, S50 - S50, 英語

    研究発表ペーパー・要旨(国際会議)

  • 4Dp02 コリネ型細菌を用いた糖を原料とするカダベリンの生産(発酵生理学,発酵工学,一般講演)

    岡井 直子, Niba Emma, 仲山 英樹, 松田 史生, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 205 - 205, 日本語

  • イオン液体耐性セルラーゼの探索と機能解析

    窪田有華, 山口剛示, 仲島菜々実, 工藤基徳, 中島一紀, 岡崎文美, 荻野千秋, 近藤昭彦

    2012年, 日本生物工学会大会講演要旨集, 64th

  • 4Ip22 イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化(バイオマス,資源,エネルギー工学,一般講演)

    小倉 一真, 仲島 菜々実, 山田 亮祐, 蓮沼 誠久, 神谷 典穂, 石田 亘広, 齋藤 聡志, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 248 - 248, 日本語

  • 4Ia02 イオン液体前処理による木質系バイオマスからのエタノール生産(バイオマス,資源,エネルギー工学,一般講演)

    石田 亘広, 片平 悟史, 齋藤 聡志, 神谷 典穂, 荻野 千秋

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 239 - 239, 日本語

  • 4Ha05 放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索(生物化学工学,一般講演)

    宮崎 貴也, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 230 - 230, 日本語

  • 4Ba09 Cell SELEX法により選抜したヒト肝臓由来がん細胞に対するDNAアプタマーの評価(セル&ティッシュエンジニアリング/生体医用工学,人工臓器,一般講演)

    川嶋 聡, 仁宮 一章, 荻野 千秋, 清水 宣明

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 186 - 186, 日本語

  • 4Dp13 エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産(発酵生理学,発酵工学,一般講演)

    野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 208 - 208, 日本語

  • イオン液体耐性セルラーゼの単離と酵素特性解析

    窪田有華, 工藤基徳, 仲島菜々実, 中島一紀, 岡崎文美, 荻野千秋, 近藤昭彦

    2012年, 酵素工学研究会講演会講演要旨集, 68th

  • 海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析

    山崎清志, 岡崎文美, 荻野千秋, 近藤昭彦, 三上文三, 榑林陽一, 鶴田宏樹

    2012年, 日本農芸化学会大会講演要旨集(Web), 2012

  • 海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性

    岡崎文美, 仲島菜々実, 荻野千秋, 近藤昭彦

    2012年, 日本農芸化学会大会講演要旨集(Web), 2012

  • 麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築

    吉栄俊秀, 青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

    2012年, 日本農芸化学会大会講演要旨集(Web), 2012

  • Streptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明

    冨田優, 松本優作, 杉森大助, 村山和隆, 荻野千秋

    2011年03月05日, 日本農芸化学会大会講演要旨集, 2011, 47, 日本語

  • 麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位VHH分泌生産系の構築

    青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

    2011年, 日本農芸化学会関西支部講演会講演要旨集, 472nd

  • 麹菌Aspergillus oryzaeによる超好熱菌Thermotoga neapolitana由来耐熱性β-1,4-マンナナーゼの生産

    岡崎文美, 仲島菜々実, 久田博元, 荻野千秋, 石田博樹, YOPI, 秦洋二, 近藤昭彦

    2011年, 日本生物工学会大会講演要旨集, 63rd

  • Affibody提示Bio-nanocapsuleを用いたHER2発現癌細胞へのタンパク質送達システム

    西村勇哉, 三村和起子, 竹田光一, 石井純, 岡崎文美, 荻野千秋, 近藤昭彦

    2011年, 日本生物工学会大会講演要旨集, 63rd

  • 麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位VHHの生産

    青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

    2011年, 日本生物工学会大会講演要旨集, 63rd

  • 2Ip13 Affibody提示Bio-nanocapsuleを用いたHER2発現癌細胞へのタンパク質送達システム(生物化学工学,一般講演)

    西村 勇哉, 三村 和起子, 竹田 光一, 石井 純, 岡崎 文美, 荻野 千秋, 近藤 昭彦

    日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 180 - 180, 日本語

  • Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの大量発現

    冨田優, 杉森大助, 荻野千秋, 松本優作

    2010年09月25日, 日本生物工学会大会講演要旨集, 62nd, 30, 日本語

  • 2P-1085 Streptomyces sriseocameus NBRC13471由来スフィンゴミエリナーゼCの大量発現(2a酸素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)

    冨田 優, 杉森 大助, 荻野 千秋, 松本 優作

    公益社団法人日本生物工学会, 2010年09月25日, 日本生物工学会大会講演要旨集, 22, 日本語

  • 2P-1086 Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの大量発現および速度論的解析(2a酸素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)

    松本 優作, 冨田 優, 杉森 大助, 荻野 千秋

    公益社団法人日本生物工学会, 2010年09月25日, 日本生物工学会大会講演要旨集, 22, 日本語

  • 超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの機能解析

    岡崎文美, 仲島菜々実, 久田博元, 荻野千秋, 石田博樹, 秦洋二, 近藤昭彦

    2010年, 日本生物工学会大会講演要旨集, 62nd

  • 麹菌による超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの高分泌生産

    久田博元, 岡崎文美, 石田博樹, 荻野千秋, 秦洋二, 近藤昭彦

    2010年, 日本生物工学会大会講演要旨集, 62nd

  • 麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの分泌高生産

    久田博元, 岡崎文美, 石田博樹, 荻野千秋, 秦洋二, 近藤昭彦

    2010年, Journal of Applied Glycoscience, 57 (Suppl.)

  • 超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質

    岡崎文美, 仲島菜々実, 久田博元, 荻野千秋, 石田博樹, 秦洋二, 近藤昭彦

    2010年, Journal of Applied Glycoscience, 57 (Suppl.)

  • Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S64 - S64, 英語

    研究発表ペーパー・要旨(国際会議)

  • Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, 英語

    研究発表ペーパー・要旨(国際会議)

  • Yuya Nishimura, Takuya Shishido, Hiroaki Mieda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S27 - S27, 英語

    研究発表ペーパー・要旨(国際会議)

  • Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, 英語

    研究発表ペーパー・要旨(国際会議)

  • Satoru Shinkawa, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S47 - S48, 英語

    研究発表ペーパー・要旨(国際会議)

  • Kazuki Nosaka, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S159 - S160, 英語

    研究発表ペーパー・要旨(国際会議)

  • Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S48, 英語

    研究発表ペーパー・要旨(国際会議)

  • Daisuke Adachi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S52, 英語

    研究発表ペーパー・要旨(国際会議)

  • Syunichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S51, 英語

    研究発表ペーパー・要旨(国際会議)

  • Hiroaki Suyama, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S49 - S49, 英語

    研究発表ペーパー・要旨(国際会議)

  • Takeyuki Tsuchidate, Toshihiro Tateno, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S49, 英語

    研究発表ペーパー・要旨(国際会議)

  • Shuhei Yanase, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S52 - S52, 英語

    研究発表ペーパー・要旨(国際会議)

  • Kazusa Matsui, Maiko Segawa, Tsutomu Tanaka, Akihiko Kondo, Chiaki Ogino

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S36 - S37, 英語

    研究発表ペーパー・要旨(国際会議)

  • Taiji Hayase, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S71 - S71, 英語

    研究発表ペーパー・要旨(国際会議)

  • Kazunori Nakashima, Shogo Arai, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S43 - S43, 英語

    [査読有り]

    研究発表ペーパー・要旨(国際会議)

  • Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, 英語

    [査読有り]

    研究発表ペーパー・要旨(国際会議)

  • Atsushi Hotta, Takanori Tanino, Tomonori Ito, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, 英語

    [査読有り]

    研究発表ペーパー・要旨(国際会議)

  • Streptomyces griseocameus NBRC13471由来スフィンゴミエリナーゼC遺伝子のクローニングと発現

    杉森大助, 冨田優, 荻野千秋, 劉暁麗

    2008年11月05日, 石油・石油化学討論会講演要旨, 38th, 211, 日本語

  • ORGN 297-Phospholipid conversion in ionic liquid by phospholipase D

    Chiaki Ogino, Toru Miyashita, Nobuaki Shimizu

    AMER CHEMICAL SOC, 2008年08月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, 英語

    研究発表ペーパー・要旨(国際会議)

  • Koki Kanehira, Toshiaki Banzai, Chiaki Ogino, Nobuaki Shimizu, Yoshinobu Kubota, Shuji Sonezaki

    Titanium dioxide (TiO2)/polyacrylic acid (PAA) (TiO2/PAA) particles were formed by mixing PAA and an acidic solution of TiO2 nanoparticles in dimethylformamide (DMF) followed by heat treatment. TEM and particle analysis showed that the resulting particles had a narrow size distribution. The colloid was very stable and aggregation was not observed over a wide pH range (3-9) or at high salt concentration. The residual carboxylic acid of PAA could be modified via EDC/NHS activation to form an amide bond with a protein. An antibody was attached to the hybrid nanoparticle and specific binding to antigen was monitored by surface plasmon resonance. The results suggest that TiO2/PAA nanoparticles are candidates as the base component of a photocatalytic system with potential for substrate selectivity. (C) 2008 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2008年06月, COLLOIDS AND SURFACES B-BIOINTERFACES, 64 (1), 10 - 15, 英語

  • Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの遺伝子クローニングと発現

    冨田優, 杉森大助, 荻野千秋, 劉暁麗

    2008年, 酵素工学研究会講演会講演要旨集, 60th, 71, 日本語

  • Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼC遺伝子のクローニングと発現系構築

    杉森 大助, 冨田 優, 荻野 千秋, 劉 暁麗

    <i>S. griseocarneus</i> NBRC13471スフィンゴミエリナーゼC遺伝子<i>smc</i>をクローニングした。次に、発現ベクターpUC702に<i>smc</i>を導入し、<i>Streptoverticillium cinnamoneum</i>を宿主として発現を行った。その結果、形質転換体の培養上清液中に約100 mg/mlの本酵素を分泌生産させることができた。しかしながら、培養上清中の酵素比活性は3 mU/mgと元株の約100分の1であった。

    公益社団法人 石油学会, 2008年, 石油学会 年会・秋季大会講演要旨集, 2008 (0), 142 - 142, 日本語

  • Tomohisa Katsuda, Chiaki Ogino, Keisuke Yamagami, Shigeo Katoh, Nobuaki Shimizu

    ELSEVIER SCIENCE BV, 2007年09月, JOURNAL OF BIOTECHNOLOGY, 131 (2), S185 - S186, 英語

    研究発表ペーパー・要旨(国際会議)

  • Chiaki Ogino, Takafumi Kubo, Kazumichi Yoshino, Nobuaki Shimizu

    ELSEVIER SCIENCE BV, 2007年09月, JOURNAL OF BIOTECHNOLOGY, 131 (2), S237 - S238, 英語

    研究発表ペーパー・要旨(国際会議)

  • ホスホリパーゼDの生産とリン脂質合成への適用

    荻野 千秋, 福田 秀樹

    バイオインダストリー協会, 2004年12月01日, バイオサイエンスとインダストリー = Bioscience & industry, 62 (12), 809 - 812, 日本語

  • バイオエコノミーの展望

    近藤昭彦, 荻野千秋

    2022年04月, 化学工学 [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化, 86 (4), 151 - 152, 日本語

    記事・総説・解説・論説等(学術雑誌)

書籍等出版物

  • Yeast Cell Surface Engineering : "Energy Production: Biodiesel"

    荻野 千秋, AMOAH JEROME

    共著, Springer Nature, 2019年05月, 英語

    学術書

  • A cancer treatment strategy that combines the use of inorganic/biocomplex nanoparticles with conventional radiation therapy, Nanoparticle Technology Handbook

    MORITA Kenta, NISHIMURA Yuya, SUZUKI T, OGINO Chiaki, KONDO Akihiko

    共著, Elsevier, 2018年03月, 英語

    学術書

  • Green Processes for Nanotechnology

    SRIVASTAVA S.K, OGINO Chiaki, KONDO Akihiko

    共著, Springer, 2015年04月, 英語

    学術書

  • 紙パルプ産業と環境 2015

    近藤 昭彦, 荻野 千秋

    共著, テックタイムス, 2014年08月, 日本語

    学術書

  • 化学便覧 応用化学編

    近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 石井 純, 原 清敬, 岡井 直子, 山田 亮祐

    共著, 丸善出版, 2014年01月, 日本語

    学術書

  • 基礎から学ぶ生物化学工学演習

    荻野 千秋

    共著, コロナ社, 2013年09月, 日本語

    学術書

  • リサイクルバイオテクノロジーの最前線, 第I編 第1章, 1 未来型CBP法によるバイオエタノール生産技術の展開

    蓮沼 誠久, 荻野 千秋, 近藤 昭彦

    共著, シーエムシー出版, 2013年05月, 日本語

    学術書

  • 次世代医薬開発に向けた抗体工学の最前線 (第Ⅱ編 抗体の改変技術,第4章 親和性の向上,第4節 「酵母による抗体フラグメントおよび抗体様結合性タンパク質の改変技術」)

    石井 純, 荻野 千秋, 近藤 昭彦

    共著, シーエムシー出版, 2012年12月, 日本語

    学術書

  • エコバイオリファイナリー

    近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 田中 勉, 中島 一紀

    共著, シーエムシー出版, 2010年12月, 日本語

    学術書

  • グリーンバイオケミストリーの最前線

    荻野 千秋, 蓮沼 誠久, 近藤 昭彦

    共著, シーエムシー出版, 2010年04月, 日本語

    学術書

  • シングルセル解析の最前線 (第3章 細胞内生体分子群の実測定量解析,第4節 「フローサイトメトリーとGFPレポーターによるG蛋白質シグナルのシングルセル解析」)

    石井 純, 田中 勉, 荻野 千秋, 近藤 昭彦

    共著, シーエムシー出版, 2010年03月, 日本語

    学術書

  • 第二世代バイオ燃料の開発と応用展開

    近藤 昭彦, 福田 秀樹, 荻野 千秋, 田中 勉, 蓮沼 誠久, 原田 和生, 福崎 英一郎

    共著, シーエムシー出版, 2009年04月, 日本語

    学術書

  • 微生物によるものづくり

    植田 充実, 芝崎 誠司, 荻野 千秋, 近藤 昭彦, 谷野 孝徳, 田中 勉, 福田 秀樹

    単著, シーエムシー出版, 2008年06月, 日本語

    学術書

  • 二酸化チタンと超音波で殺菌?!

    荻野 千秋

    単著, 学進出版, 2008年02月, 日本語

    学術書

  • 進化・増殖する化学工学

    荻野 千秋, 近藤 昭彦

    共著, 関西化学機械製作株式会社創立60周年記念出版, 2008年02月, 日本語

    学術書

  • 微生物を用いたバイオ・リファイナリー戦略:バイオマスからの燃料・化学品の生産

    荻野 千秋, 近藤 昭彦

    共著, ケミカルエンジニアリング, 2008年, 日本語

    一般書・啓蒙書

  • 二酸化チタン・超音波触媒法による細胞死誘導

    高木 圭子, 荻野 千秋, 清水 宣明

    共著, 超音波テクノ, 2007年09月, 日本語

    学術書

  • 二酸化チタン・超音波触媒法を応用した有害微生物殺菌システム

    荻野 千秋, Mohammad Mizanur Rahaman, 清水宣明

    共著, 超音波テクノ, 2007年07月, 日本語

    学術書

  • 二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果

    清水 宣明, 荻野 千秋, 藤平 敦司

    共著, 超音波テクノ, 2007年07月, 日本語

    学術書

  • 二酸化チタン・超音波触媒法の環境浄化、殺菌への応用

    清水 宣明, 荻野 千秋

    共著, 空気清浄, 2007年05月, 日本語

    学術書

  • ナノテクノロジーによる新規がん治療法.

    清水 宣明, 荻野 千秋, 高木 圭子, 山岸 紗也花, 野瀬 律子

    共著, 最新医学, 2006年06月, 日本語

    学術書

  • 二酸化チタンと超音波で殺菌?

    荻野 千秋

    単著, 生物工学会誌, 2006年01月, 日本語

    学術書

  • 超音波利用技術集成「超音波と二酸化チタンを用いた有害微生物の殺菌」

    清水 宣明, 荻野 千秋

    共著, NTS, 2005年04月, 日本語

    学術書

  • 二酸化チタンへの超音波照射によって発生するラジカルの応用

    清水 宣明, 荻野 千秋, MF Dadjour

    共著, マテリアルインテグレーション, 2005年03月, 日本語

    学術書

  • 二酸化チタン・超音波照射による大腸菌殺菌メカニズム

    清水 宣明, 荻野 千秋, MF Dadjour

    共著, 化学工業, 2005年02月, 日本語

    学術書

  • ホスホリパーゼDの生産とリン脂質合成への適用

    荻野 千秋, 福田 秀樹

    共著, バイオサイエンスとインダストリー, 2004年12月, 日本語

    学術書

  • Stress-induced serotonin release in the hypothalamus and suppression of food intake in rats

    N. Shimizu, 荻野 千秋

    共著, Recent Research Developments in Physiology, 2003年, 英語

    学術書

  • テッシュエンジニアリングの展望

    紀ノ岡 正博, 荻野 千秋

    共著, 三恵社, 2002年03月, 日本語

    学術書

  • Functional analysis of phospholipase D and its application for phospholipids synthesis

    荻野 千秋

    単著, INSEF, 2002年, 英語

    学術書

  • エコエネルギー

    荻野 千秋

    単著, 生物工学会誌, 2000年07月, 日本語

    学術書

講演・口頭発表等

  • 油脂酵母Lipomyces starkeyi の遺伝子的改変による長鎖長脂肪酸の生産

    宮本 捺央, Amza Rezky Lastinov, Prihardi Kahar, 荻野 千秋, 近藤 昭彦

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 日本酒生産にヒントを得た麹菌酵素カクテルと酵母を用いた高純度希少糖生産

    若井 暁, 堤 浩子, 秦 洋二, 荻野 千秋, 近藤 昭彦

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 新奇な好熱性放線菌Streptomyces thermoviolaceus を用いたタンパク質生産株の開発

    齊藤 朱音, 三原 笑, 曽田 匡洋, 荻野 千秋, 柏木 紀賢, 竹中 武藏, 近藤 昭彦

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 酵母カルチャーコレクションからのバイオエタノール生産のための優良酵母の選抜と評価

    荻野 千秋, Prihardi Kahar, 近藤 昭彦

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 好冷性海洋細菌Psychroflexus torquis 由来β-1,3-キシラナーゼの酵素化学的諸性質

    谷口 瑞季, 工藤 基徳, 荻野 千秋, 近藤 昭彦, 田丸 浩, 青木 恭彦, 岡﨑 文美

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 好熱性放線菌Streptomyces thermoviolaceus による物質生産

    三原 笑, 竹中 武藏, 柏木 紀賢, 曽田 匡洋, 荻野 千秋, 近藤 昭彦

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 基質酵素反応予測のための 機械学習に基づく予測モデルの構築方法の探索と評価

    渡邉 直暉, 村田 昌浩, 荻野 千秋, 近藤 昭彦, 荒木 通啓

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 過酸化チタンナノ粒子 (PAA -TiOx )の 細胞内傷害

    小寺澤 翔太, 荻野 千秋, 西村 勇哉, 近藤 昭彦

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • イオン液体で前処理した木質系バイオマス中の多糖およびリグニンのリファイナリー

    廣部 綾乃, 柘植 陽太, 荻野 千秋, 高橋 憲司, 仁宮 一章

    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 新奇な好熱性放線菌Streptomyces thermoviolaceusを用いたタンパク質生産株の開発

    齊藤 朱音, 三原 笑, 竹中 武藏, 柏木 紀賢, 荻野 千秋, 近藤 昭彦

    第21回化学工学会学生発表会, 2019年03月, 日本語, 京都大学桂キャンパス, 国内会議

    ポスター発表

  • 完全分散型麹菌のプロテオーム解析

    櫻川 拓, 片山 周平, 張 斯来, 若井 暁, 荻野 千秋, 近藤 昭彦

    第21回化学工学会学生発表会, 2019年03月, 日本語, 京都大学桂キャンパス, 国内会議

    ポスター発表

  • ピルビン酸代謝フロー改変を用いた 2,3-ブタンジオール 高生産麹菌の構築

    張 斯来, 若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    日本農芸化学会2019年度大会, 2019年03月, 日本語, 東京農業大学 世田谷キャンパス, 国内会議

    ポスター発表

  • ナノポアシーケンサを用いた遺伝子組換え麹菌のゲノミ クスおよびトランスクリプトミクス

    若井 暁, 張 斯来, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    日本農芸化学会2019年度大会, 2019年03月, 日本語, 東京農業大学 世田谷キャンパス, 国内会議

    ポスター発表

  • PEG化チタンナノ粒子の種々の表面修飾による特性解析

    小寺澤 翔太, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第21回化学工学会学生発表会, 2019年03月, 日本語, 京都大学桂キャンパス, 国内会議

    ポスター発表

  • Bio-refinery strategy by collaboration Asian's partnership

    Chiaki OGINO, Prihardi Kahar, Akihiko KONDO

    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019年, APCChE, Sapporo Convention Center

  • Direct increase of intracellular hydrogen peroxide and enhancement for radiosensitivity with polyacrylic acid-modified titanium peroxide nanoparticles

    Chiaki OGINO, Kenta MORITA, Yuya NISHIMURA, Chiya NUMAKO, Masao NAKAYAMA, Ryohei SASAKI, Akihiko KONDO

    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019年, APCChE, Sapporo Convention Center

  • 乳酸生産麹菌のピルビン酸ミトコンドリアキャリアタンパク質欠損における代謝変動

    張 斯来, 若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第18回糸状菌分子生物学コンファレンス, 2018年11月, 日本語, シティホールプラザ「アオーレ長岡」, 国内会議

    ポスター発表

  • 外来遺伝子発現用のコムギ細胞外排出シグナルと乾燥誘導性プロモーターの効果

    大野 良子, 久野 裕, 山根 美樹, 佐藤 和広, 荻野 千秋, 近藤 昭彦, 宅見 薫雄

    第13回ムギ類研究会, 2018年11月, 日本語, 横浜市立大学木原生物学研究所, 国内会議

    ポスター発表

  • 黄麹菌における汎用性高発現プロモーター

    片山 周平, 張 斯来, 若井 暁, 堤 浩子, 秦 洋二, 荻野 千秋, 近藤 昭彦

    第18回糸状菌分子生物学コンファレンス, 2018年11月, 日本語, シティホールプラザ「アオーレ長岡」, 国内会議

    ポスター発表

  • マルチコピー遺伝子導入黄麹菌のゲノム構造解析

    若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第18回糸状菌分子生物学コンファレンス, 2018年11月, 日本語, シティホールプラザ「アオーレ長岡」, 国内会議

    ポスター発表

  • バイオマスから基礎化学品原料をつくる(2)

    平野 喜章, 奥田 典正, 高橋 典, 有吉 公男, 荻野 千秋, 喜多 裕一

    異業種交差点Ⅱ, 2018年11月, 日本語, 新化学技術推進協会, 一橋講堂 2階中会議室, 国内会議

    ポスター発表

  • Target cancer therapy by inorganic nanoparticle

    MORITA Kenta, SUZUKI Takahiro, NISHIMURA Yuya, OGINO Chiaki, KONDO Akihiko

    The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), 2018年11月, 英語, National Taiwan University, 国際会議

    口頭発表(一般)

  • Caffeic acid production from lignocellulosic biomass by metabolically engineered Eshrichaia coli

    KAWAGUCHI Hideo, SAZUKA Takashi, KATSUYAMA Yohei, TERAMOTO Jun, NISHIDA Keiji, MINAMI Hiromichi, OGINO Chiaki, OHNISHI Yasuo, KONDO Akihiko

    The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), 2018年11月, 英語, National Taiwan University, 国際会議

    口頭発表(一般)

  • 油脂酵母Lipomyces starkeyi D35株の 繰り返し発酵による脂肪酸組成変化解析

    宮本 捺央, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 免疫クリアランスを回避する生体膜由来ドラッグデリバリーキャリアの開発

    赤嶺 藍, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 乳酸高生産性黄麹菌における乳酸生産フラックス強化による乳酸生産性の向上

    張 斯来, 若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析

    富永 大輔, 川口 秀夫, 堀 良美, 蓮沼 誠久, 荻野 千秋, 油谷 幸代

    第70回日本生物工学会大会, 2018年09月, 日本語, 日本生物工学会, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 組換えコリネ型細菌を用いた3-アミノ-4-ヒドロキシ安息香酸の発酵生産における酸素制限による 促進作用

    川口 秀夫, 宮崎 翔子, 荻野 千秋, 大西 康夫, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 最少量の低毒性イオン液体を用いた前処理に基づく木質系バイオマスリファイナリー

    仁宮 一章, Amaliyah Rohsari, Indah Utami, 柘植 陽太, 荻野 千秋, 髙橋 憲司

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 最少量の低毒性イオン液体を用いたバイオマスからバイオ燃料へのHigh-loadingワンポット変換

    杉野 雄規, 柘植 陽太, 荻野 千秋, 髙橋 憲司, 仁宮 一章

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 麹菌内で同調的発現挙動を示すプロモーターを用いたタンパク質生産挙動の解析

    片山 周平, 張 斯来, 若井 暁, 堤 浩子, 秦 洋二, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 好熱性放線菌Streptomyces thermoviolaceus の培養特性解析

    三原 笑, 竹中 武藏, 柏木 紀賢, 曽田 匡洋, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 原子間力顕微鏡を用いたB型肝炎感染機構の解明

    西田 真一朗, 竹中 武藏, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 機械学習を用いた酵素のEC number予測

    渡邉 直暉, 村田 昌浩, 荻野 千秋, 近藤 昭彦, 荒木 通啓

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • バイオマス分解酵素の大規模スクリーニングで見出した希少放線菌

    竹中 武藏, Lee Jae Min, Pamella Apriliana, 柏木 紀賢, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • ナノポアシーケンサを用いた遺伝子組換え麹菌ゲノム上の長鎖タンデムリピート構造の解析

    若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • The enhancement of culture conditions for high cell and lipid production of oleaginous yeast Lipomyces starkeyii D35

    Rezky Lastinov Amza, Prihardi Kahar, Ario Betha Juanssilfero, Hiromi Otsuka, Chie Kihira, Chiaki Ogino, Akihiko Kondo

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Selection of oleaginous yeast capable of high lipid accumulating during challenges from inhibitory chemical complexes

    Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice by flocculating yeast Saccharomyces cerevisiae

    Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Preparation and characterisation of robust whole cell biocatalyst from Aspergillus oryzae expressing Fusarium heterosporum lipase immobilized onto activated carbon for biodiesel synthesis

    Emmanuel Quayson, Jerome Amoah, Akihiko Kondo, Shinji Hama, Ayumi Yoshida, Chiaki Ogino

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Optimization on biodiesel synthesis from palm oil mill effluent (POME) by integrated strategy with lipasecatalyzed ethanolysis

    Nova Rachmadona, Jerome Amoah, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • mRNAサイレンシングを用いた過酸化チタンナノ粒子の放射線増感機序解明

    大取 靖秀, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Improvement of mass mixing by Max-Blend and anchor shape impeller in the high-solid state scarification of kraft pulp

    Deddy Triyono, Nugroho Adi, Chiaki Ogino, Akihiko Kondo

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Effect of immobilized Chlorella Sorokiniana culture on lipid accumulation and CO2 sequestration

    Daniel Alejandro Alfaro Sayes, Jerome Amoah, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • Dilute maleic acid pretreatment of sugarcane bagasse and oil palm empty fruit bunch fiber for enhanced enzymatic digestibility

    Lucky Risanto, Deddy Triyono, Nugroho Adi, Triyani, Hiroshi Teramura, Euis Hermiati, Chiaki Ogino, Akihiko Kondo

    第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議

    ポスター発表

  • 免疫クリアランスの回避を目指した生体膜由来キャリアの開発

    赤嶺 藍, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第34回日本DDS学会学術集会, 2018年06月, 日本語, 長崎ブリックホール, 国内会議

    ポスター発表

  • 新規放射線増感剤としての過酸化チタンナノ粒子の物性解析

    森田 健太, 西村 勇哉, 大取 靖秀, 荒井 優紀, 沼子 千弥, 荻野 千秋

    第34回日本DDS学会学術集会, 2018年06月, 日本語, 長崎ブリックホール, 国内会議

    ポスター発表

  • 過酸化チタンナノ粒子の併用による放射線増感治療法の開発

    西村 勇哉, 森田 健太, 荻野 千秋, 犬伏 祥子, 佐々木 良平, 近藤 昭彦

    第34回日本DDS学会学術集会, 2018年06月, 日本語, 長崎ブリックホール, 国内会議

    ポスター発表

  • バイオマスから基礎化学品原料をつくる

    平野 喜章, 奥田 典正, 高橋 典, 有吉 公男, 荻野 千秋, 喜多 裕一

    第7回JACI/GSCシンポジウム, 2018年06月, 日本語, 新化学技術推進協会, ANA CROWNE PLAZA KOBE, 国内会議

    ポスター発表

  • Converting Chlamydomonas sp. JSC4 lipids to biodiesel via biocatalysis with Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell

    JEROME Amoah, SHIH-HSIN. Ho, SHINJI Hama, AYUMI Yoshida, AKIHITO Nakanishi, TOMOHISA Hasunuma, CHIAKI Ogino, AKIHIKO Kondo

    The 8th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2018), 2018年06月, 英語, Motif Seattle, 国際会議

    ポスター発表

  • 多孔体粒子を用いた固定化培養槽の撹拌性能調査

    三原 裕香, Narges Ghobadi, 大村 直人, 荻野 千秋, 堀江 孝史

    化学工学会,第83年会, 2018年03月, 日本語, 関西大学, 国内会議

    ポスター発表

  • 酸素をスイッチとしたコリネ型細菌中央代謝経路の切り替え

    小林 俊介, 岩崎 還帰, 川口 秀夫, 仁宮 一章, 荻野 千秋, 高橋 憲司, 近藤 昭彦, 柘植 陽太

    日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議

    ポスター発表

  • 麹菌ミトコンドリア; ピルビン酸キャリアタンパク質欠損株におけるピルビン酸代謝フラックス改変による有用化合物生産

    張 斯来, 若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議

    ポスター発表

  • 酵素改変を指向したin silico解析

    若井 暁, 荻野 千秋, 近藤 昭彦

    第3回デザイン生命工学研究会, 2018年03月, 日本語, デザイン生命工学研究, 沖縄県国頭郡今帰仁村, 国内会議

    ポスター発表

  • リグノセルロース系バイオマスを包括的に利用したコリネ型細菌によるバイオモノマー生産

    川口 秀夫, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議

    ポスター発表

  • リグノセルロースナノファイバー表面のリグニン分布解析手法の開発

    冨田 康平, 竹中 武藏, 森田 健太, 川口 秀夫, 荻野 千秋, 近藤 昭彦

    日本化学会第98春季年会2018, 2018年03月, 日本語, 日本化学会, 船橋市, 国内会議

    ポスター発表

  • Saccharomyces cerevisiae酵母におけるCandida boidinii由来キシロース発酵代謝系の導入効果

    Kahar Prihardi, 紀平 知枝, 大塚 裕美, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議

    ポスター発表

  • 同時糖化発酵プロセスを利用した紙パルプを原料とするカフェ酸の生産

    川口 秀夫, 勝山陽平, ト タンヤオ, 鶴田 祥子, 南 博道, 荻野 千秋, 大西康夫, 近藤 昭彦

    第13回バイオマス科学会議, 2018年01月, 英語, 日本エネルギー学会, 仙台市, 国際会議

    口頭発表(一般)

  • Efficient Xylitol Production from Kraft Pulp by a Cell Surface Engineered Strain ofSaccharomyces cerevisiae

    Gregory Guirimand, 猪熊 健太郎, 番場 崇弘, 佐々木 建吾, 荻野 千秋, 蓮沼 誠久, 近藤 昭彦

    5th Asian Conference on Biomass Science, 2018年01月, 英語, 日本エネルギー学会, 仙台市, 国際会議

    ポスター発表

  • 麦わらのバイオマス利用に関するパンコムギ系統間差異

    大野 良子, 寺村 浩, 荻野 千秋, 近藤 昭彦, 宅見 薫雄

    第12回ムギ類研究会, 2017年12月, 日本語, 京都大学, 国内会議

    ポスター発表

  • 麹菌のミトコンドリア局在ピルビン酸キャリアタンパク質欠損による乳酸生産性の向上

    張 斯来, 若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第17回糸状菌分子生物学コンファレンス, 2017年11月, 日本語, 糸状菌分子生物学研究会, 佐賀市, 国内会議

    口頭発表(一般)

  • 麹菌の co-transformation 時のコピー数多型性に関する解析

    若井 暁, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第17回糸状菌分子生物学コンファレンス, 2017年11月, 日本語, 糸状菌分子生物学研究会, 佐賀市, 国内会議

    口頭発表(一般)

  • リグニン残量を制御したセルロースから得られた各種ナノファイバーの構造と物性

    村上 大祐, 松本 拓也, 大島 智子, 寺村 浩, 川口 秀夫, 荻野 千秋, 近藤 昭彦, 西野 孝

    第7回CSJ化学フェスタ2017, 2017年10月, 日本語, 日本化学会, 東京都, 国内会議

    ポスター発表

  • In situ detection of an arming yeast cellulase using AFM

    荻野 千秋

    The 23th Symposium of Young Asian Biochemical Engineers' Communyty (YABEC2017), 2017年10月, 英語, Xi'an, China, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 発酵阻害物質耐性Candida boidinii K212のキシロース発酵の解析

    Kahar Prihardi, 紀平 知枝, 大塚 裕美, 荻野 千秋, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • 発酵阻害耐性酵母由来強力なENO1プロモーターの発現解析

    Nurlina B. Azmi, Prihardi Kahar, 糸見 明穂, 紀平 知枝, 荻野 千秋, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • 酸素をスイッチとした二つの樹脂原料の個別生産

    小林 俊介, 柘植 陽太, 岩崎 還帰, 川口 秀夫, 仁宮 一章, 荻野 千秋, 高橋 憲司, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • 麹菌による複数遺伝子の同時高発現を可能にするプロモーターセットの開発

    片山 周平, 若井 暁, 堤 浩子, 秦 洋二, 荻野 千秋, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • 高度膜分離技術によるソルガム搾汁液からのスクロース分離および連続的エタノール生産

    佐々木 建吾, 川口 秀夫, 荻野 千秋, 佐塚 隆志, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    口頭発表(一般)

  • The establishment high cell density culture of oleaginous Lipomyces starkeyi D35 for high cell and lipid production

    Rezky Lastinov Amza, Prihardi Kahar, Ario Betha Juanssilfero, Hiromi Otsuka, Chie Kihira, Chiaki Ogino, Akihiko Kondo

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • High Production of single cell oil from glucose and xylose using oleaginous yeast Lipomyces starkeyi

    Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • Exploration of potential indigenous marine actinomycetes from indonesia soil producing enzyme lignocellulosic for biorefinery application

    Apriliana Pamella, Nanik Rahmani, Fahrurrozi Izzuddin, Puspita Lisdiyanti, Jaemin Lee, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • Enhanced cell surface engineering of Saccharomyces cerevisiae and optimization of the fermentation process for efficient xylitol production from kraft pulp

    Gregory Guirimand, 猪熊 健太郎, 番場 崇弘, 佐々木 建吾, 荻野 千秋, 蓮沼 誠久, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    口頭発表(一般)

  • B型肝炎ウイルス由来ペプチドを用いたAFMによる肝細胞の受容体検出

    西田 真一朗, 竹中 武藏, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議

    ポスター発表

  • リグニン量を制御したセルロースナノファイバーの構造と物性

    村上 大祐, 松本 拓也, 大島 智子, 寺村 浩, 川口 秀夫, 荻野 千秋, 近藤 昭彦, 西野 孝

    第63回高分子研究発表会(神戸), 2017年07月, 日本語, 高分子学会関西支部, 神戸市, 国内会議

    口頭発表(一般)

  • リグニン含有量を異にするセルロースから得られた各種ナノファイバーの構造と物性

    村上 大祐, 松本 拓也, 大島 智子, 寺村 浩, 川口 秀夫, 荻野 千秋, 近藤 昭彦, 西野 孝

    平成29年度繊維学会年次大会, 2017年06月, 日本語, 繊維学会, 東京都, 国内会議

    ポスター発表

  • 組換え大腸菌によるグルコースを原料とする4-アミノ 桂皮酸の直接発酵生産

    川口 秀夫, 若井桂子, 桝尾俊介, 高谷直樹, 荻野 千秋, 近藤 昭彦

    日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • 肝細胞認識ペプチドを用いた AFMによる肝癌細胞の力学的評価

    西田 真一朗, 鈴木 貴弘, 森田 健太, 竹中 武蔵, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第19回化学工学会学生発表会, 2017年03月, 日本語, 化学工学会関西支部, 豊中市, 国内会議

    口頭発表(一般)

  • 遺伝子組み換え麹菌培養上清カクテルを用いた希少糖高生産プロセスの開発

    若井 暁, 浅井菜々実, 荻野 千秋, 堤浩子, 秦洋二, 松沢智彦, 矢追克郎, 近藤 昭彦

    日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • バイオマスの効率的な分解を志向したマルチセルラーゼ高発現麹菌の開発

    片山 周平, 若井 暁, 浅井 菜々実, 堤 浩子, 秦 洋二, 荻野 千秋, 近藤 昭彦

    第19回化学工学会学生発表会, 2017年03月, 日本語, 化学工学会関西支部, 豊中市, 国内会議

    口頭発表(一般)

  • デザインされた生体分子の触診デバイス

    竹中 武藏, 荻野 千秋, 近藤 昭彦

    第2回デザイン生命工学研究会大会, 2017年03月, 日本語, デザイン生命工学研究会, 神戸市, 国内会議

    口頭発表(一般)

  • コリネ型細菌を用いたフェルラ酸からプロトカテク酸の生産

    岡井直子, 増田敬也, 竹島康誠, 田中耕生, 吉田健一, 宮本正教, 荻野 千秋, 近藤 昭彦

    日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • REDOXバランスにおける発酵阻害耐性酵母由来新規TDHxの影響

    Azmi Nurlina, 糸見 明穂, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

    第19回化学工学会学生発表会, 2017年03月, 日本語, 化学工学会関西支部, 豊中市, 国内会議

    口頭発表(一般)

  • デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果

    森田 隆太郎, 寺村 浩, 荻野 千秋, 近藤 昭彦, 深山 浩

    日本作物学会講演会, 2017年, 日本語, 国内会議

    ポスター発表

  • 代謝改変およびフラックス強化した黄麹菌でのデンプンからの乳酸生産

    笹倉 直也, 若井 暁, 浅井菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議

    口頭発表(一般)

  • 麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立

    荒添 貴之, 西田 敬二, 田畑 麻由良, 若井 暁, 荻野 千秋, 秦 洋二, 近藤 昭彦

    第16回糸状菌分子生物学カンファレンス, 2016年11月, 日本語, 宇治おうばくプラザ, 国内会議

    ポスター発表

  • 麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立

    荒添貴之, 西田敬二, 田畑麻由良, 若井 暁, 荻野 千秋, 秦 洋二, 近藤 昭彦

    第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議

    口頭発表(一般)

  • 黄麹菌 Aspergillus oryzae による機能性オリゴ糖の生産

    若井 暁, 浅井菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議

    口頭発表(一般)

  • マルチセルラーゼ発現黄麹菌の培養条件によるセルラーゼ生産性の違い

    片山周平, 若井 暁, 浅井菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議

    口頭発表(一般)

  • 疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発

    寺村浩, 佐々木 建吾, 白井智量, 川口 秀夫, 荻野 千秋, 菊地淳, 佐塚隆志, 近藤 昭彦

    第61回リグニン討論会, 2016年10月, 日本語, リグニン討論会実行委員会, 宇治市, 国内会議

    口頭発表(一般)

  • Mapping of endoglucanases displayed on a surface of yeast cell catalyst using atomic force microscopy

    荻野 千秋

    The 22th Symposium of Young Asian Biochemical Engineers' Communyty, 2016年10月, 英語, 生物工学会, 宮崎市, 国際会議

    口頭発表(一般)

  • 転写因子改変放線菌を用いた異種タンパク質生産

    柏木 紀賢, 西岡 雅都, 廣瀬 修一, 曽田 匡洋, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明

    鈴木 貴弘, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • 新規放射線増感治療に向けた過酸化チタンナノ粒子のマウス生体内分布特性の調査

    鈴木 貴弘, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議

    口頭発表(一般)

  • 酸化チタン類ナノ粒子と物理的外部刺激を併用した新規がん治療の提案

    荻野 千秋, 森田 健太, 鈴木 貴弘, 西村 勇哉, 中山雅央, 佐々 木良平, 近藤 昭彦, 沼子千弥

    セラミック協会第 29 回秋季シンポジウム, 2016年09月, 日本語, セラミック協会, 東広島市, 国内会議

    口頭発表(一般)

  • 三種セルラーゼ共・強発現麹菌によるセルロースからのコウジ酸生産

    若井 暁, 浅井 菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • 原料となる植物バイオマスの種類がセルロー スナノファイバーの強度に与える影響の解析

    寺村 浩, 延田 紘治, 荻野 千秋, 北野 英己, 平野 恒, 佐塚 隆志, 西野 孝, 近藤 昭彦

    第34回日本植物細胞分子生物学会(上田)大会, 2016年09月, 日本語, 日本植物細胞分子生物学会, 長野県上田市, 国内会議

    口頭発表(一般)

  • 凝集性酵母における新規な阻害剤耐性機構の解明

    糸見 明穂, 紀平 和枝, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • 遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析

    宮川 寛規, 川口 秀夫, 荻野 千秋, 老沼 研一, 高谷 直樹, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • σ因子欠損放線菌の3,4-AHBA生産への影響

    釜我 圭, 柏木 紀賢, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • バイオナノカプセルとリポソームの複合粒子によるin vivoでの抗腫瘍効果

    西村 勇哉, 鈴木 貴弘, 森田 健太, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • ナノ粒子とX線照射の併用による低侵襲がん治療法の開発

    森田 健太, 西村 勇哉, 鈴木 貴弘, 荻野 千秋, 近藤 昭彦

    化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議

    口頭発表(一般)

  • コリスミ酸ピルビン酸リアーゼ発現コリネ型細菌を用いたプロトカテク酸の生産

    岡井 直子, 三好 孝則, 竹嶋 康誠, 田中 耕生, 吉田 健一, 桑原 広明, 荻野 千秋, 近藤 昭彦

    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議

    口頭発表(一般)

  • アルコールの添加が希硫酸前処理の効率に与える影響の解析

    荻野 千秋, 寺村 浩, 佐々木 建吾, 川口 秀夫, 佐塚 隆志, 近藤 昭彦

    化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議

    口頭発表(一般)

  • 膵臓がん治療に向けた新規放射線増感剤としての過酸化チタンナノ粒子のin vitro評価

    森田 健太, 鈴木 貴弘, 西村 勇哉, 松本和久, 沼子千弥, 佐藤和好, 中山雅央, 佐々木良平, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議

    ポスター発表

  • 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明

    鈴木 貴弘, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議

    ポスター発表

  • HER2受容体発現細胞を標的としたDDSキャリアの抗腫瘍効果の評価

    西村 勇哉, 鈴木 貴弘, 森田 健太, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議

    ポスター発表

  • AFMで診る・識る・釣る ~核酸・タンパク・細胞の研究~

    竹中 武藏, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議

    ポスター発表

  • ブタノール/硫酸法によりリグニン含有量を調節したリグノセルロースナノファイバーシートの物性

    松本 拓也, 小林(岡久) 陽子, 寺村 浩, 大島 智子, 森田 健太, 本郷 千鶴, 川口 秀夫, 荻野 千秋, 近藤 昭彦, 西野 孝

    第5回 JACI/GSCシンポジウム, 2016年06月, 日本語, 公益社団法人 新化学技術推進協会, 神戸市, 国内会議

    ポスター発表

  • アルコールの添加が希硫酸前処理の効率に与える影響の解析

    寺村 浩, 川口 秀夫, 佐塚 隆志, 荻野 千秋, 近藤 昭彦

    第5回JACI GSCシンポジウム, 2016年06月, 日本語, 新化学技術推進協会, 神戸市, 国内会議

    ポスター発表

  • Optimization of Displayed Cellulases in Yeast Suitable for Ionic Liquid Pretreated Biomass by Cocktail δ Integration Methodology

    荻野 千秋, Naoya Ishizue, 近藤 昭彦

    Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議

    口頭発表(一般)

  • Engineering and Analyzing Streptomyces strain for the Recombinant Protein Production

    Norimasa Kashiwagi, Satoko Niimi-Nakamura, 広瀬 修一, Masahiro Sota, 荻野 千秋, 近藤 昭彦

    Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議

    口頭発表(一般)

  • Direct Fermentation of L-Lactic Acid from Starch By Genetically Engineered Aspergillus Oryzae

    若井 暁, 笹倉 直也, 浅井 菜々美, Hiroko Tsutumi, Yoji Hata, 荻野 千秋, 近藤 昭彦

    Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議

    口頭発表(一般)

  • Development of Platform Yeast Strain Capable of Direct Fermentation of Raw Biomass to Ethanol

    Prihardi Kahar, Akiho Itomi, Ahmad Thontowi, Hiromi Otsuka, Chie Kihira, Jaemin Lee, Ario Betha Juanssil fero, Apridah Cameliawati Djohan, Yopi Sunarya, Jun Ishii, Chiaki Ogino, Banbang Prasetya, Akihiko Kondo

    Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議

    ポスター発表

  • Caffeic Acid Production By Simultaneous Saccharification and Fermentation of Kraft Pulp Using Recombinant Escherichia coli

    川口 秀夫, 勝山 洋平, Du Danyao, Sachiko Nakamura-Tsuruta, Hiroshi teramura, Hiromichi Minami, 荻野 千秋, Yasuo Ohnishi, 近藤 昭彦

    Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議

    口頭発表(一般)

  • 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査

    鈴木 貴弘, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    化学工学会第81年会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • 紙パルプの同時糖化発酵によるカフェ酸の微生物生産

    川口 秀夫, 勝山 洋平, トタンヤオ, 鶴田 祥子, 南 博道, 荻野 千秋, 大西 康夫, 近藤 昭彦

    日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • マンナンバイオマスからのエタノール生産:βーマンナナーゼとβーマンノシダーゼを細胞表層に提示した出芽酵母の開発

    石井 純, 岡崎 文美, Apridah Cameliawati, Djohan, 原 清敬, 浅井 菜々実, Ade Andriani, 寺村 浩, Prihardi Kahar, YOPI, Bambang Prasetya, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • コリネ型細菌によるUV吸収性アミノ酸の生産

    柘植 陽太, 山本 省吾, 増田 匡洋, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • アルコールの添加物が希硫酸前処理の効率に与える影響の解析

    寺村 浩, 佐々木 健吾, 白井 智量, 川口 秀夫, 荻野 千秋, 菊池 淳, 佐塚 隆志, 近藤 昭彦

    日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • From mannan and lignocellulosic biomass to biochemicals: cell surface display and metabolic engineering in yeast Saccharomyces cerevisiae

    Jun Ishii, Fumiyoshi Okazaki, Shuhei Ishikado, Apridah Cameliawati Djohan, Nanami Asai-Nakashima, Yopi, Fumio Matsuda, Chiaki Ogino, Akihiko Kondo

    The 6th iBioK Asian Workshop, 2016年03月, 日本語, Kobe, 国際会議

    口頭発表(一般)

  • AFM力計測を用いた細胞表層提示酵素の特異的検出とマッピング評価

    荻野 千秋, 竹中 武藏, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦

    化学工学会第81年会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • AFM力学計測法を利用した細胞表層空間に提示した酵素検出

    竹中 武藏, 荻野 千秋, 近藤 昭彦, 猪熊 健太郎, 蓮沼 誠久, 小林 拓也

    日本化学会第96春季年回, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • Affibody提示バイオナノカプセル/リポソーム融合粒子を用いたin vivo での抗腫瘍効果

    西村 勇哉, 鈴木 貴弘, 森田 健太, 荻野 千秋, 近藤 昭彦

    化学工学会第81年会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産

    川口 秀夫, 寺村 浩, 中村 聡子, 荻野 千秋, 原 清敬, 蓮沼 誠久, 老沼 研一, 高谷 直樹, 平野 亘, 佐塚 隆志, 北野 英己, 近藤 昭彦

    第11回バイオマス科学会議, 2016年01月, 日本語, 国際会議

    口頭発表(一般)

  • 稲わらのバイオリファイナリーに向けた特性の解析と多様性の評価

    合田 喬, 寺村 浩, 末廣 美紀, 金丸 研吾, 川口 秀夫, 荻野 千秋, 近藤 昭彦, 山崎 将紀

    神戸大学若手フロンティア2015, 2015年12月, 日本語, 神戸大学, 国内会議

    ポスター発表

  • バイオリファイナリー利用に向けた稲わらの希硫酸前処理後グルコース含量の自然変異

    合田 喬, 寺村 浩, 末廣 美紀, 金丸 研吾, 川口 秀夫, 荻野 千秋, 近藤 昭彦, 山崎 将紀

    日本農芸化学会関西支部第492例会, 2015年12月, 日本語, 神戸大学, 国内会議

    口頭発表(一般)

  • Specific drug delivery for target cancer tumor using affibody-displaying bionanocapsule/liposome complex

    Yuya Nishimura, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), 2015年12月, 英語, 国際会議

    口頭発表(一般)

  • Live cell imaging and membrane protein mapping with atomic force microscope

    Musashi Takenaka, T. Kobayashi, Yusuke Miyachi, Kentarou Inokuma, Jun Ishii, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

    The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), 2015年12月, 英語, 国際会議

    口頭発表(一般)

  • Development of novel radiosensitizing cancer therapy:Combination of radio-therapy and titanium peroxide nanoparticle

    Morita Kenta, Suzuki Takahiro, Nakayama M, Sasaki R, Sato K, Numako Chiya, Ogino Chiaki, Kondo Akihiko

    Pacifichem 2015, 2015年12月, 英語, 国際会議

    口頭発表(一般)

  • Characterization for metal-oxide nano-perticles generating radicals with X-ray irrdiatio

    Numako Chiya, Miyazaki S, Sato K, Takami S, Ogino Chiaki, Kondo Akihiko

    Pacifichem 2015, 2015年12月, 英語, 国際会議

    口頭発表(一般)

  • コピー数多形とプロモーター強度を加味した黄麹菌でのマルチ遺伝子発現

    若井 暁, 浅井 菜々美, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第15回糸状菌分子生物学コンファレンス, 2015年11月, 日本語, 国内会議

    口頭発表(一般)

  • L-乳酸生産黄麹菌の乳酸生産能に対する中央代謝経路改変の影響

    笹倉 直也, 若井 暁, 浅井 菜々美, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第15回糸状菌分子生物学コンファレンス, 2015年11月, 日本語, 国内会議

    口頭発表(一般)

  • 代謝改変による赤色酵母を用いたアスタキサンチン生産性の向上

    山元 啓輔, 原清敬, 荻野 千秋, 近藤 昭彦

    第67回日本生物工学会大会 国際シンポジウム, 2015年10月, 日本語, 国内会議

    口頭発表(一般)

  • Streptoverticillium Cinnamoneum由来Phospholipase D プロモーター遺伝子発現機構の調査

    柏木 紀賢, 西岡 雅都, 松本 華, 広瀬 修一, 増田 匡洋, 荻野 千秋, 近藤 昭彦

    第67回日本生物工学会大会 国際シンポジウム, 2015年10月, 日本語, 国内会議

    口頭発表(一般)

  • L-乳酸生産株Aspergillus oryzaeの代謝改変によるLー乳酸生産能の改変

    笹倉 直也, 若井 暁, 浅井 菜々美, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第67回日本生物工学会大会 国際シンポジウム, 2015年10月, 日本語, 国内会議

    口頭発表(一般)

  • Lignocellulosic biomass refinery using biocompatible ionic liquida

    荻野 千秋, 仁宮 一章, 覚知 亮平, 遠藤 太佳嗣, 黒田 浩介, 野口 愛, 清水 信明, 高橋 憲司

    The 21th Symposium of Young Asian Biochemical Engineer's Communyty, 2015年10月, 英語, 国際会議

    口頭発表(一般)

  • Conversion of high phospholipid-containing oils to biodiesel using immobilizes Aspergillus oryzae whole cell biocatalyst ezpressing Fusarium heteroporum lipase

    Jerom Amoah, shin-Hsin Ho, Hama Shinji, Yoshida Ayumi, Nakanishi Akihito, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko

    The 21th Symposium of Yong Asian Biochemical engineer's Communyty, 2015年10月, 英語, 国際会議

    口頭発表(一般)

  • 放射線治療と相乗効果を有するポリアクリル酸修飾過酸化チタンナノ粒子の作用機序解明

    森田 健太, 鈴木 貴弘, 西村 勇哉, 沼子 千弥, 佐藤 和好, 中山 雅央, 佐々木 良平, 荻野 千秋, 近藤 昭彦

    化学工学会第47回秋季大会, 2015年09月, 日本語, 国内会議

    口頭発表(一般)

  • 超音波照射によるリグニン分解の反応速度解析

    鳥羽 由里菜, 堀江 孝史, 田中 皓己, 荻野 千秋, 大村 直人

    化学工学会 第47回秋季大会, 2015年09月, 日本語, 化学工学会, 北海道大学, 国内会議

    口頭発表(一般)

  • 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査

    鈴木 貴弘, 森田 健太, 西村 勇哉, 荻野 千秋, 近藤 昭彦

    化学工学会第47回秋季大会, 2015年09月, 日本語, 国内会議

    口頭発表(一般)

  • バイオマスからの微生物によるバイオモノマー生産

    荻野 千秋

    マテリアルサイエンス研究科セミナー, 2015年07月, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Screening of ethanol and lactic acid-producing yeast from Indonesia culture collection

    Ahamad Thontowi, Senlie Octaviana, Atit Kanti, Ario Betha Juanssifero, Yopi, Bambang Prasetya, Prihardi Kahar, Ogino Chiaki, Kondo Akihiko

    6th AFOB Regional Symposium, 2015年05月, 英語, Asian Federation of Biotechnology, 国際会議

    口頭発表(一般)

  • Screening and characterization of Lignocellulose-hydrolytic from Indonesia Rare Actinomycetes and applications of these enzymes for sugar cane baggase saccharification to produce bioethanol

    Nanik Rahmani, Pamela Apriliana, Alifah M. Jannah, Shanti Ratnakomala, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Jaemin Lee, Prihardi Kahar, Ogino Chiaki, Kondo Akihiko

    6th AFOB Regional Symposium, 2015年05月, 英語, Asian Federation of Biotechnology, 国際会議

    口頭発表(一般)

  • 膜濃縮したソルガム搾汁液からのエタノール発酵

    佐々木 建吾, 柘植 陽太, 佐々木 大介, 寺村 浩, 川口 秀夫, 荻野 千秋, 春日 重光, 佐塚 隆志, 近藤 昭彦

    日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議

    口頭発表(一般)

  • 担癌マウスへのTiナノ粒子投与による生体内分布

    鈴木 貴弘, 田野 亜都里, 森田 健太, 荻野 千秋, 近藤 昭彦

    第17回化学工学会学生発表会, 2015年03月, 日本語, 化学工学会, 徳島市, 国内会議

    口頭発表(一般)

  • 組換えコリネ型細菌による3-hydroxy-4-aminobenzoic acid発酵におけるソルガム搾汁液の促進作用

    川口 秀夫, 佐々木 建吾, 寺村 浩, 柘植 陽太, 中村 聡子, 菅井 佳宜, 勝山 洋平, 大西 康夫, 平野 恒, 佐塚 隆志, 北野 英巳, 春日 重光, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議

    口頭発表(一般)

  • 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

    KAHAR Prihardi, 李 載みん, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議

    口頭発表(一般)

  • コリネ型細菌を用いたプロトカテク酸の生産

    岡井 直子, 竹嶋 康誠, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議

    口頭発表(一般)

  • AFM による細胞表層提示タンパク質の特異的検出およびマッピング

    竹中 武藏, 小林 拓也, 荻野 千秋, 近藤 昭彦

    日本化学会第95春季年会, 2015年03月, 日本語, 日本化学会, 船橋市, 国内会議

    口頭発表(一般)

  • Construction of arming yeast displaying optimal ratio of cellulases for degradation of biomass pretreated with ionic liquid

    OGINO Chiaki, ISHIZUE Naoya, YASUDA Misa, ISHIZAKI Miki, NINOMIYA Kazunori, KONDO Akihiko

    Active Enzyme Molecule 2014, 2014年12月, 英語, 酵素工学会, 富山市, 国内会議

    ポスター発表

  • 麹菌細胞工場を用いた L- および D-乳酸の生産

    笹倉 直也, 若井 暁, 浅井 菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第14回糸状菌分子生物学コンファレンス, 2014年11月, 日本語, 糸状菌分子生物学研究会, 仙台市, 国内会議

    ポスター発表

  • 好熱性および好冷性酵素のドメインを持つキメラ酵素の構築

    若井 暁, 浅井 菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第15回極限環境生物学会年会, 2014年11月, 日本語, 極限環境生物学会, 沖縄市, 国内会議

    ポスター発表

  • セルラーゼ遺伝子をマルチコピー導入した麹菌の遺伝子コピー数とセルラーゼ活性

    若井 暁, 浅井 菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第14回糸状菌分子生物学コンファレンス, 2014年11月, 日本語, 糸状菌分子生物学研究会, 仙台市, 国内会議

    ポスター発表

  • Microbial screening for biorefinery research

    OGINO Chiaki

    The 20th Symposium of Young Asian Biochemical Engineers' Communyty, 2014年11月, 英語, 生物工学会, Taiwan, China, 国際会議

    口頭発表(一般)

  • イオン液体前処理後バガスに適したアーミング酵母の開発

    石居 直也, 石崎 美紀, 安田 美砂, 仁宮 一章, 高橋 憲司, 荻野 千秋, 近藤 昭彦

    第5回イオン液体討論会, 2014年10月, 日本語, イオン液体研究会, 横浜市, 国内会議

    口頭発表(一般)

  • 放線菌を用いた糖を原料とするパラアミノ安息香酸の生産

    岡井 直子, 佐藤 嘉弘, 大野 摩耶, 竹嶋 康誠, 増田 敬哉, 宮本 正教, 樋田 幸三, 荻野 千秋, 近藤 昭彦

    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議

    ポスター発表

  • 麹菌を用いたデンプンからのD- 乳酸の同時糖化発酵

    若井 暁, 浅井 菜々実, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議

    ポスター発表

  • 稲わら前処理液の膜プロセスによるエタノール発酵高効率化

    佐々木 建吾, 柘植 陽太, 佐々木 大介, 荻野 千秋, 近藤 昭彦

    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議

    ポスター発表

  • バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響

    川口 秀夫, 寺村 浩, 中村 聡子, 荻野 千秋, 原 清敬, 蓮沼 誠久, 老沼 研一, 高谷 直樹, 平野 恒, 佐塚 隆志, 北野 英己, 近藤 昭彦

    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議

    ポスター発表

  • pH 応答性ペプチドGALA を表層提示したバイオナノカプセルのエンドソーム脱出

    西村 勇哉, 江澤 僚将, 石井 純, 荻野 千秋, 近藤 昭彦

    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議

    ポスター発表

  • pH応答性膜融合ペプチドGALAを表層提示したバイオナノカプセルのエンドソーム脱出

    西村 勇哉, 竹田 光一, 江澤 僚将, 石井 純, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議

    ポスター発表

  • 放射線がん治療との併用に向けたか酸化チタンナノ粒子の物性評価

    森田 健太, 田野 亜都里, 鈴木 貴弘, 沼子 千弥, 宮嵜 世里加, 佐藤 和好, 中山 雅央, 佐々木 良平, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議

    ポスター発表

  • 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発

    江澤 僚将, 西村 勇哉, 石井 純, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議

    ポスター発表

  • リグノセルロース系バイオマスからのバイオ芳香族化合物の生産

    川口 秀夫, 寺村 浩, 中村 聡子, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議

    ポスター発表

  • カーボンナノチューブの表面改質を目指した重合性界面活性剤の開発

    舟崎 裕一, 本庄 崇文, 竹中 武蔵, 荻野 千秋, 丸山 達生

    第60回高分子研究発表会(神戸), 2014年07月, 日本語, 高分子学会, 神戸市, 国内会議

    ポスター発表

  • AFMを用いた表層提示酵母へのアプローチ及び表層提示酵素の検出

    竹中 武蔵, 小林 拓矢, 荻野 千秋, 近藤 昭彦

    生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議

    ポスター発表

  • カーボンナノチューブの可溶化を目指した重合性界面活性剤の開発

    舟崎 裕一, 本庄 崇文, 竹中 武蔵, 荻野 千秋, 丸山 達生

    第63回高分子学会年次大会, 2014年05月, 日本語, 高分子学会, 名古屋市, 国内会議

    ポスター発表

  • 放線菌による 3,4-AHBA 合成とキシロース代謝

    中村 聡子, 川口 秀夫, KAWAGUCHI Hide, 荻野 千秋, 菅井 佳宣, 勝山 陽平, 大西 康夫, 近藤 昭彦

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • 同時糖化発酵による希硫酸前処理バイオマスからのフェニル乳酸生産

    川口 秀夫, 寺村 浩, 中村 聡子, 荻野 千秋, 老沼 研一, 高谷 直樹, 平 野 恒, 佐塚 隆志, 北野 英己, 近藤 昭彦

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議

    口頭発表(一般)

  • 新しい放射線治療をめざした過酸化チタンナノ粒子の物性研究

    森田 健太, 田野 亜都里, 沼子 千弥, 佐藤 和好, 中山 雅央, 佐々木 良平, 荻野 千秋, 近藤 昭彦

    化学工学会第79年会, 2014年03月, 日本語, 化学工学会, 岐阜市, 国内会議

    ポスター発表

  • バイオマス評価法の開発

    寺村 浩, 山崎 将紀, 川口 秀夫, 合田 喬, 松田 史生, 荻野 千秋, 近藤 昭彦

    第55回日本植物生理学会年会, 2014年03月, 日本語, 富山, 国内会議

    ポスター発表

  • タンパク質提示酵母の表層における親和力解析

    小林 拓矢, 荻野 千秋, 近藤 昭彦

    第16回化学工学会学生発表会(堺大会), 2014年03月, 日本語, 化学工学会, 大阪市, 国内会議

    口頭発表(一般)

  • タンパク高生産放線菌のゲノム変異解析と因子探索

    中村 聡子, 廣瀬 修一, 西岡 雅都, 柏木 紀賢, 曽田 匡洋, 荻野 千秋, 近藤 昭彦

    第8回日本ゲノム微生物学会年会, 2014年03月, 日本語, 日本ゲノム微生物学会, 世田谷区, 国内会議

    ポスター発表

  • セルロース系バイオマスからの物質生産を可能にしたスーパー麹菌の開発

    若井 暁, 浅井 菜々実, 山田 亮祐, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議

    口頭発表(一般)

  • コリネ型細菌を用いた糖を原料とするGABAの生産

    岡井 直子, 高橋 千尋, 畑田 一樹, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議

    口頭発表(一般)

  • イオン液体を用いたリグノセルロースのトータルリファイナリー

    仁宮 一章, 荻野 千秋, 清水 宣明, 高橋 憲司

    化学工学会第79年会, 2014年03月, 日本語, 化学工学会, 岐阜市, 国内会議

    口頭発表(一般)

  • イオン液体と酸を用いた新規バイオマス前処理法の開発と応用

    小倉 一真, 荻野 千秋, 近藤 昭彦

    化学工学会第79年会, 2014年03月, 日本語, 化学工学会, 岐阜市, 国内会議

    ポスター発表

  • 低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵

    表 小百合, 仁宮 一章, 荻野 千秋, 高橋 憲司, 清水 宣明

    第4回イオン液体研究会, 2013年11月, 日本語, イオン液体研究会, 横浜市, 国内会議

    ポスター発表

  • 放線菌による芳香族化合物ホモゲンチジン酸の生産

    中村 聡子, 川口 秀夫, 荻野 千秋, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 同時糖化発酵によるセルロースからのフェニル乳酸生産

    川口 秀夫, 中村 聡子, 荻野 千秋, 老沼 研一, 高谷 直樹, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵

    表 小百合, 仁宮 一章, 曽田 裕司, 荻野 千秋, 高橋 憲司, 清水 宣明

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 低コストイオン液体によるエタノール製造プロセスの提案

    齋藤 聡志, 石田 亘広, 神谷 典穂, 荻野 千秋

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 新しい重合性界面活性剤を用いた難溶性ナノ材料可溶化技術の開発

    舟崎 裕一, 本庄 崇文, 宝得 一貴, 南 秀人, 竹中 武蔵, 荻野 千秋, 丸山 達生

    第62回高分子討論会, 2013年09月, 日本語, 高分子学会, 金沢市, 国内会議

    口頭発表(一般)

  • 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発

    江澤 僚将, 西村 勇哉, 石井 純, 荻野 千秋, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 酸触媒イオン液体前処理を用いた新規バイオプロセス構築

    小倉 一真, 荻野 千秋, 近藤 昭彦

    日本農芸化学会関西;中四国;西日本支部合同大会(第481回講演会), 2013年09月, 日本語, 日本農芸化学会, 広島市, 国内会議

    口頭発表(一般)

  • 麹菌Aspergillus oryzae によるL-乳酸の生産

    若井 暁, 吉栄 俊秀, 浅井 菜々実, 山田 亮祐, 荻野 千秋, 堤 浩子, 秦 洋二, 近藤 昭彦

    日本農芸化学会関西;中四国;西日本支部合同大会(第481回講演会), 2013年09月, 日本語, 日本農芸化学会, 広島市, 国内会議

    口頭発表(一般)

  • 各種イネ科植物から作製したセルロースナノファイバーシートの物性評価

    延田 紘治, 寺村 浩, 荻野 千秋, 近藤 昭彦, 西野 孝, 平野 恒, 佐塚 隆志, 北野 英己

    平成25年度繊維学会秋季研究発表会, 2013年09月, 日本語, 名古屋市, 国内会議

    ポスター発表

  • 稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮

    佐々木 建吾, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 易糖化性を示すイネの探索

    寺村 浩, 平野 恒, 松田 史生, 荻野 千秋, 佐塚 隆志, 北野 英己, 近藤 昭彦

    第31回日本植物細胞分子生物学会大会;シンポジウム, 2013年09月, 日本語, 日本植物細胞分子生物学会, 札幌市, 国内会議

    口頭発表(一般)

  • バイオリファイナリーを目指したイオン液体の活用

    石田 亘広, 齋藤 聡志, 神谷 典穂, 荻野 千秋

    化学工学会第45回秋季大会, 2013年09月, 日本語, 化学工学会, 岡山市, 国内会議

    ポスター発表

  • バイオマス分解性向上を目的とする酸導入イオン液体を用いた新規前処理法開発

    小倉 一真, 荻野 千秋, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • ナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発

    田野 亜都里, 森田 健太, 荻野 千秋, 佐藤 和好, 沼子 千弥, 中山 雅央, 佐々木 良平, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • コリネ型細菌を用いたプロトカテク酸の生産

    岡井 直子, 竹嶋 康誠, 荻野 千秋, 近藤 昭彦

    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議

    ポスター発表

  • 各種イネ科植物から作製したセルロースナノファイバーシートの物性

    延田 紘治, 寺村 浩, 荻野 千秋, 近藤 昭彦, 西野 孝, 平野 恒, 佐塚 隆志, 北野 英己

    セルロース学会第20回年次大会, 2013年07月, 日本語, 京都市, 国内会議

    ポスター発表

  • 植物源を異にするセルロースナノファイバーシートの構造と物性

    延田 紘治, 平野 恒, 佐塚 隆志, 北野 英己, 寺村 浩, 荻野 千秋, 近藤 昭彦, 西野 孝

    第2回JACI/GSC 講演会, 2013年06月, 日本語, 新化学技術推進協会, 大阪市, 国内会議

    ポスター発表

  • イオン液体を用いたバイオマスの前処理と同時糖化発酵

    荻野 千秋, 近藤 昭彦

    第2回JACI/GSC 講演会, 2013年06月, 日本語, 新化学技術推進協会, 大阪市, 国内会議

    ポスター発表

  • 重合性界面活性剤による難溶性材料可溶化技術の開発

    本庄 崇文, 宝得 一貴, 南 秀人, 竹中 武蔵, 荻野 千秋, 丸山 達生

    化学工学会 第78年会, 2013年03月, 日本語, 化学工学会, 大阪大学, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いた細胞表層解析法および細胞応答解析法の開発

    竹中 武蔵, 荻野 千秋

    日本化学会第93春季年会, 2013年03月, 日本語, 日本化学会, 草津市, 国内会議

    口頭発表(一般)

  • Ethanol production from ionic liquid pretreated biomass by recombinant yeast

    OGINO Chiaki, KONDO Akihiko

    化学工学会第78年会, 2013年03月, 日本語, 化学工学会, 豊中市, 国内会議

    口頭発表(一般)

  • コリン系イオン液体と超音波を組み合わせた木質系バイオマス前処理

    仁宮一章, 荻野 千秋

    第3回イオン液体討論会, 2012年12月, 日本語, イオン液体研究会, 那覇市, 国内会議

    口頭発表(一般)

  • イオン液体耐性セルラーゼの単離と酵素特性解析

    窪田 有華, 仲島 菜々実, 工藤 基徳, 中島 一紀, 岡﨑 文美, 荻野 千秋

    第3回イオン液体討論会, 2012年12月, 日本語, イオン液体研究会, 那覇市, 国内会議

    口頭発表(一般)

  • Interaction survey between GPCR and ligand on the surface of yeast by AFM

    Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

    International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies), 2012年11月, 英語, The Society for Single-Cell Surveyor, 京都市, 国際会議

    ポスター発表

  • 放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索

    宮崎 貴也, 野田 修平, 田中 勉, 荻野 千秋

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • ピリジニウム系イオン液体による結晶性セルロースのin situ 酵素糖化

    中元 亜耶, 石田 亘広, 荻野 千秋

    酵素工学研究会第68回講演会, 2012年10月, 日本語, 酵素工学研究会, 文京区, 国内会議

    ポスター発表

  • エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

    野田 修平, 田中 勉, 荻野 千秋

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

    野田 修平, 田中 勉, 荻野 千秋

    酵素工学研究会第68回講演会, 2012年10月, 日本語, 酵素工学研究会, 文京区, 国内会議

    ポスター発表

  • イオン液体耐性セルラーゼの探索と機能解析

    窪田 有華, 山口 剛示, 仲島 菜々実, 工藤 基徳, 中島 一紀, 岡﨑 文美, 荻野 千秋

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • イオン液体耐性セルラーゼの単離と酵素特性解析

    窪田 有華, 工藤 基徳, 仲島 菜々実, 中島 一紀, 岡﨑 文美, 荻野 千秋

    酵素工学研究会第68回講演会, 2012年10月, 日本語, 酵素工学研究会, 文京区, 国内会議

    ポスター発表

  • イオン液体前処理による木質系バイオマスからのエタノール生産

    石田 亘広, 片平 悟史, 齋藤 聡志, 神谷 典穂, 荻野 千秋

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化

    小倉 一真, 仲島 菜々実, 山田 亮祐, 蓮沼 誠久, 神谷 典穂, 石田 亘広, 齋藤 聡志, 荻野 千秋

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • Genetic engineering of valine biosynthesis for isobutanol production in Saccharomyces cerevisiae

    Jun Ishii, Fumio Matsuda, Kengo Ida, Hironori Tezuka, Takashi Kondo, Chiaki Ogino, Akihiko Kondo

    The 18th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012), 2012年10月, 英語, Society for Biological Engineering, Tokushima, Japan, 国際会議

    口頭発表(一般)

  • Direct ethanol production from lignocellulosic biomass pretreated with ionic liquid from cellulase-displaying yeast

    OGINO Chiaki

    The 18th Symposium of Young Asian Biochemical Engineers' Communyty, 2012年10月, 英語, ㈳日本生物工学会, 徳島市, 国際会議

    ポスター発表

  • Cell SELEX 法により選抜したヒト肝臓由来がん細胞に対するDNA アプタマーの評価

    川嶋 聡, 仁宮 一章, 荻野 千秋

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • Complex carriers of affibody-displaying bio-nanocapsule and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery

    Yuya Nishimura, Jun Ishii, Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo

    The 15th International Biotechnology Symposium (IBS 2012), 2012年09月, 英語, The Korean Society for Biotechnology and Bioengineering, Deague, Korea, 国際会議

    口頭発表(一般)

  • Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production by Saccharomyces cerevisiae

    Jun Ishii, Takashi Kondo, Hiroyuki Tezuka, Fumio Matsuda, Chiaki Ogino, Akihiko Kondo

    Metabolic EngineeringⅨ, 2012年06月, 英語, Engineering Conferences International, Biarritz, France, 国際会議

    口頭発表(一般)

  • AFMを用いた新規核酸分子の探索

    荻野 千秋

    21世紀を拓くバイオテクノロジーシンポジウム, 2012年06月, 日本語, YABEC2012実行委員会, 徳島市, 国内会議

    口頭発表(一般)

  • ラジカルを発生する金属酸化物粒子に対するXAFSによる非破壊状態分析

    沼子 千弥, 佐藤 和好, 高見 誠一, 梅津 光央, 田中 勉, 荻野 千秋

    第72回分析化学討論会, 2012年05月, 日本語, 日本分生化学会, 鹿児島市, 国内会議

    口頭発表(一般)

  • DNA aptamer screening and evaluation by AFM

    OGINO Chiaki, AMINO Tomokazu, KONDO Akihiko

    The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, 2012年05月, 英語, 酵素工学研究会, 金沢市, 国際会議

    口頭発表(一般)

  • 動的周期操作を用いたエタノール連続発酵プロセスにおける分岐現象

    濱島 奈生子, 堀江 孝史, 荻野 千秋, 大村 直人

    化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築

    吉栄 俊秀, 青木 淳一, 田淵 聡一郎, 岡崎 文美, 荻野 千秋, 田中 勉, 久田 博元, 秦 洋二, 近藤 昭彦

    日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いた細胞表層におけるレセプター・リガンド間相互作用測定系の構築

    竹中 武藏, 荻野 千秋, 宮地 佑典, 近藤 昭彦

    日本化学会第92春季年会, 2012年03月, 日本語, 日本化学会, 横浜市, 国内会議

    口頭発表(一般)

  • 海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性

    岡崎 文美, 仲島 菜々実, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • 海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析

    山崎 清志, 岡崎 文美, 荻野 千秋, 近藤 昭彦, 三上 文三, 榑林 陽一, 鶴田 宏樹

    日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • ピリジニウム系イオン液体による結晶性セルロースの前処理・酵 素糖化・高速発酵(その2)

    荻野 千秋, 山口 剛示, 仲島 菜々美, 片平 悟史, 石田 亘広, 徳原 渡, 神谷 典穂, 近藤 昭彦

    化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議

    口頭発表(一般)

  • ピリジニウム系イオン液体による結晶性セルロースの前処理・酵素糖化・高速発酵(その1)

    中元 亜耶, 庄田 靖宏, 引野 幸枝, 片平 悟史, 石田 亘広, 徳原 渡, 荻野 千秋, 神谷 典穂

    化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議

    口頭発表(一般)

  • ソマトスタチンに対する新規DNA アプタマーの選抜およびセンシングへの応用

    荻野 千秋, 網野 智一, 宮地 佑典, 近藤 昭彦

    日本化学会第92春季年会, 2012年03月, 日本語, 日本化学会, 横浜市, 国内会議

    口頭発表(一般)

  • セルロースからの高効率一段階エタノール発酵を可能にする高機能酵母の創製

    中谷 侑紀, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議

    口頭発表(一般)

  • コリネ型細菌を用いたプトレシンの生産

    岡井 直子, 畑田 一樹, 高橋 千尋, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • エンドグルカナーゼ分泌生産型放線菌を用いたセルロースからの有用タンパク質生産

    河井 禎文, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

    野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議

    口頭発表(一般)

  • リグノセルロース・リファイナリーにおけるイオン液体の役割

    仁宮 一章, 荻野 千秋, 高橋 憲司

    第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議

    口頭発表(一般)

  • イオン液体耐性セルラーゼの探索と機能評価

    窪田 有華, 山口 剛示, 仲島 菜々実, 中島 一紀, 岡﨑 文美, 荻野 千秋, 近藤 昭彦

    第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議

    口頭発表(一般)

  • イオン液体前処理バイオマスを用いたエタノール同時糖化発酵

    荻野 千秋, 中島 一紀, 仲島 菜々美, 神谷 典穂, 徳原 渡, 石田 亘広, 片平 悟史, 近藤 昭彦

    第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議

    口頭発表(一般)

  • イオン液体前処理バイオマスの構造解析およびエタノール発酵

    山口 剛示, 中島 一紀, 荻野 千秋, 山田 亮祐, 堀江 孝史, 桑本 滋生, 横山 和司, 松井 純爾

    第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議

    口頭発表(一般)

  • イオン液体共存下における酵母のキシロース発酵能評価

    小倉 一真, 仲島 菜々実, 山田 亮祐, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦

    第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議

    口頭発表(一般)

  • Amplification and smoothing of signal activation from human G-protein-coupled receptor for enriching agonist stimulation in yeast

    小田 麻美, 石井 純, 福田 展雄, 藤原 俊伸, 荻野 千秋, 近藤 昭彦

    第34回日本分子生物学会年会, 2011年12月, 英語, 日本分子生物学会, 横浜市, 国内会議

    ポスター発表

  • 超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの結晶構造解析

    岡崎 文美, 山崎 清志, 荻野 千秋, 近藤 昭彦, 三上文三, 榑林陽一, 鶴田宏樹

    極限環境生物学会2011年度(第12回)年会, 2011年11月, 日本語, 極限環境生物学会, 長崎市, 国内会議

    ポスター発表

  • 超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの安定性解析

    岡崎 文美, 仲島 菜々実, 荻野 千秋, 近藤 昭彦

    極限環境生物学会2011年度(第12回)年会, 2011年11月, 日本語, 極限環境生物学会, 長崎市, 国内会議

    ポスター発表

  • 組換え麹菌生産セルラーゼを用いた広葉樹クラフトパルプの酵素糖化

    吉栄 俊秀, 酒井 翔司, 岡﨑 文美, 荻野 千秋, 田中 勉, 久田 博元, 萩原 伸哉, 秦 洋二, 近藤 昭彦

    第11回糸状菌分子生物学コンファレンス(2011), 2011年11月, 日本語, 糸状菌分子生物学研究会, 文京区, 国内会議

    ポスター発表

  • ラクダ由来一本鎖抗体可変部位 VHH の麹菌 Aspergillus oryzae による分泌生産

    青木 淳一, 田淵 聡一郎, 岡﨑 文美, 荻野 千秋, 田中 勉, 久田 博元, 秦 洋二, 近藤 昭彦

    第11回糸状菌分子生物学コンファレンス(2011), 2011年11月, 日本語, 糸状菌分子生物学研究会, 文京区, 国内会議

    ポスター発表

  • 放線菌が有する炭素源資化能力を生かしたタンパク質の大量分泌生産

    宮崎 貴也, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議

    ポスター発表

  • 組換え酵母による高効率グルタチオン生産

    桐山 健太郎, 原 清敬, 仲山 英樹, 荻野 千秋, 近藤 昭彦

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • 生体分子融合ナノ粒子の生物化学工学領域への高度応用に関する研究

    荻野 千秋

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 麹菌Aspergillus oryzae による超好熱菌Thermotoga neapolitana 由来耐熱性β-1,4- マンナナーゼの生産

    岡﨑 文美, 仲島 菜々実, 久田 博元, 荻野 千秋, 石田 博樹, Yopi, 秦 洋二, 近藤 昭彦

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • 酵母によるバイオマスからのグルタチオンの直接発酵

    原 清敬, 吉田 秀世, 桐山 健太郎, 金 松希, 荻野 千秋, 近藤 昭彦

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • 稲わらバイオマス形質の系統間比較

    松田 史生, 山崎 将紀, 荻野 千秋, 近藤 昭彦

    第29回日本植物細胞分子生物学会大会, 2011年09月, 日本語, 日本植物細胞分子生物学会, 福岡市, 国内会議

    口頭発表(一般)

  • βグルコシダーゼを表層提示したセロオリゴ糖資化性大腸菌の創製

    田中 勉, 川畑 瞳, 平田 有希, 荻野 千秋, 近藤 昭彦

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議

    ポスター発表

  • セルラーゼ分泌酵母によるイオン液体処理バイオマスからのエタノール生産

    片平 悟史, 中村 里沙, 徳原 渡, 則武 義幸, 中島 一紀, 神谷 典穂, 荻野 千秋, 石田 亘広

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • セルラーゼ発現最適化二倍体酵母による木質系バイオマスからのエタノール発酵

    山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議

    ポスター発表

  • イオン液体処理が結晶性セルロース酵素糖化に与える影響

    中元 亜耶, UJU, 庄田 靖宏, 後藤 雅宏, 石田 亘広, 徳原 渡, 則武 義幸, 荻野 千秋, 神谷 典穂

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発

    荻野 千秋, 中島 一紀, 山口 剛示, 窪田 有華, 片平 悟史, 石田 亘広, 徳原 渡, 則武 義幸, 近藤 昭彦

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • Corynebacterium glutamicumを用いた高効率なGABA生産技術の開発

    高橋 千尋, 白川 順規, 畑田 一樹, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議

    ポスター発表

  • BGL 表層提示大腸菌によるセロビオースを基質としたIPA 生産

    相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 花井 泰三

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • Affibody 提示Bio-nanocapsule を用いたER2 発現癌細胞へのタンパク質送達システム

    西村 勇哉, 三村 和起子, 竹田 光一, 石井 純, 岡崎 文美, 荻野 千秋, 近藤 昭彦

    第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議

    口頭発表(一般)

  • Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans

    OGINO Chiaki

    Defence, Science & Research (DSR 2011) Conference, 2011年08月, 英語, Committee of DSR2011, Singapore, 国際会議

    口頭発表(一般)

  • バイオナノカプセルを用いた細胞特異的ドラッグデリバリーシステム

    近藤 昭彦, 田中 勉, 荻野 千秋

    第27回日本DDS学会学術集会, 2011年06月, 日本語, 日本DDS学会, 文京区, 国内会議

    口頭発表(一般)

  • Key Monomers Production from Biomass Toward Bio-based Polymers Using

    OGINO Chiaki

    International Conference on Materials for Asdvanced Technologies 2011, 2011年06月, 英語, Materials Research Society Singapore, Singapore, 国際会議

    口頭発表(一般)

  • イオン液佒へのセルロースの溶解とバイオエタノール生産

    中島 一紀, 山口 剛示, 片平 悟史, 石田 亖広, 荻野 千秋, 近藤 昭彦

    第60回高分子学会年次大会, 2011年05月, 日本語, 高分子学会, 大阪市, 国内会議

    口頭発表(一般)

  • Homo D-lactic acid fermentation from pentose and xylan by metabolically engineered Lactobacillus plantarum

    TANAKA Tsutomu, OKANO Kenji, OGINO Chiaki, KONDO Akihiko

    33rd Symposium on Biotechnology for Fuels and Chemicals, 2011年05月, 英語, Society for industrial Microbiology, Seattle, USA, 国際会議

    ポスター発表

  • Effective Screening of Optimized Diploid Saccharomyces cerevisiae Strain for Direct Ethanol Production from Cellulosic Materials by Displaying Ratio Adjustment of Cellulolytic Enzymes

    OGINO Chiaki

    2011 International Meeting of the Microbiological Society of Korea, 2011年05月, 英語, The Microbiological Society of Korea, Gwangju, Korea, 国際会議

    口頭発表(一般)

  • Cinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividans

    NODA Shuhei, MIYOSHI, Takanori, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

    33rd Symposium on Biotechnology for Fuels and Chemicals, 2011年05月, 英語, Society for industrial Microbiology, Seattle, USA, 国際会議

    ポスター発表

  • Characterization of Titanium oxide nano-particle suspension by XAFS technique

    NUMAKO C, SATO K, OHARA S, UMETSU M, TAKAMI S, SASAKI R, OGINO Chiaki, KONDO Akihiko

    E-MRS ICAM IUMRS 2011 Spring Meeting, 2011年05月, 英語, European Materials Research Society, Nice, France, 国際会議

    口頭発表(一般)

  • 放線菌を用いたあらゆる炭素源からのケイ皮酸生産

    野田 修平, 三好 孝則, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • 放線菌によるトランスグルタミナーゼの大量分泌生産に向けた生産条件の検討

    宮崎 貴也, 野田 修平, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • 放線菌によるトランスグルタミナーゼの生産

    宮崎 貴也, 野田 修平, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 放線菌における表層提示技術に関する研究

    中村 悠佑, 野田 修平, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製

    山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • 代謝工学を活用したイソブタノール生産酵母の創製

    手塚 裕紀, 近藤 貴志, 石井 純, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • 組換え酵母による高効率グルタチオン生産

    桐山 健太郎, 原 清敬, 仲山 英樹, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • 酵母を用いたバイオマス資源からのグルタチオン生産技術の開発

    Kim Songhee, 吉田 秀世, 原 清敬, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 酵母を用いたバイオマスからのグルタチオンの生産

    原 清敬, 吉田 秀世, 桐山 健太郎, Kim Songhee, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • 酵素活性を制御するDNAアプタマーの探索

    宮地 佑典, 荻野 千秋, 近藤 昭彦

    日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いた機能性核酸分子の選抜

    奥村 雄三, 荻野 千秋, 宮地 佑典, 近藤 昭彦

    日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いた機能性核酸分子の選抜

    奥村 雄三, 荻野 千秋, 宮地 佑典, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いたアミノ酸に対する機能性核酸分子・アプタマーの新規選抜法の開発

    荻野 千秋, 早瀬 太治, 宮地 佑典, 近藤 昭彦

    日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発

    森田 健太, 唐崎 美樹, 佐藤 和好, 大原 智, 中山 雅央, 佐々木 良平, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発

    森田 健太, 唐崎 美樹, 佐藤 和好, 大原 智, 中山 雅央, 佐々木 良平, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • βグルコシダーゼ表層提示によるセロビオース資化性大腸菌の創製

    田中 勉, 川畑 瞳, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • βグルコシダーゼを表層提示したセロビオース資化性大腸菌の創製

    田中 勉, 川畑 瞳, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • ランダム変異導入による高活性エンドグルカナーゼの創製

    中谷 侑紀, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • トランスポゾンタギング法による好塩性細菌Halomonas elongata OUT30018株の浸透圧ストレス応答性遺伝子の探索

    谷村 幸亮, 仲山 英樹, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • ソマトスタチンに対する新規DNAアプタマーの選抜

    網野 智一, 荻野 千秋, 宮地 佑典, 近藤 昭彦

    日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • コリネ型細菌を用いたGABAの生産

    高橋 千尋, 白川 順規, 畑田 一樹, 土舘 健幸, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • イオン液体共存下でのセルロース系バイオマスからの直接エタノール発酵

    中島 一紀, 谷口 菜穂, 山口 剛示, 山田 亮祐, 片平 悟史, 石田 亘広, 徳原 渡, 則武 義幸, 荻野 千秋, 近藤 昭彦

    日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • イオン液体を利用したバイオマスの前処理・糖化・発酵技術

    石田 亘広, 片平 悟史, 中村 里沙, 徳原 渡, 則武 義幸, 神谷 典穂, 中島 一紀, 荻野 千秋, 近藤 昭彦, 高橋 治雄

    日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • イオン液体をベースとしたセルロース系バイオマスの前処理とエタノール発酵プロセス

    中島 一紀, 谷口 菜穂, 山口 剛示, 山田 亮祐, 片平 悟史, 石田 亘広, 徳原 渡, 則武 義幸, 荻野 千秋, 近藤 昭彦

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • イオン液体によるバイオマスの前処理・糖化・発酵技術の開発

    石田 亘広, 片平 悟史, 中村 里沙, 徳原 渡, 則武 義幸, 神谷 典穂, 中島 一紀, 荻野 千秋, 近藤 昭彦, 高橋 治雄

    化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議

    口頭発表(一般)

  • Streptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明

    冨田 優, 松本 優作, 杉森 大助, 村山 和隆, 荻野 千秋

    日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議

    口頭発表(一般)

  • Single-cell analysis revealed correlation between yeast GPCR signaling and plasmid retention

    Jun Ishii, Chiaki Ogino, Akihiko Kondo

    The 5th International Workshop on Approaches to Single-Cell Analysis, 2011年03月, 英語, Tokyo, Japan, 国際会議

    ポスター発表

  • Corynebacterium glutamicumを用いたGABAの高効率生産技術の開発

    高橋 千尋, 白川 順規, 畑田 一樹, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • AFMを用いた生細胞表層のレセプター・リガンド間相互作用解析と細胞応答の観察

    荻野 千秋, 野坂 和輝, 石井 純, 宮地 佑典, 近藤 昭彦

    日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • 植物から燃料を作る

    荻野 千秋

    芦屋川カレッジ, 2011年02月, 日本語, 芦屋市公民館, 芦屋市, 国内会議

    口頭発表(招待・特別)

  • 金属酸化ナノ粒子と物理的励起を併用したラジカル発生システムを用いた細胞損傷システムの構築

    荻野 千秋

    第23回CES21講演会, 2011年02月, 日本語, 日本分子生物学会, 大阪市, 国内会議

    口頭発表(招待・特別)

  • High-resolution, quantitative signalling assay for G-protein-coupled receptors by flow cytometry

    Akihiko Kondo, Jun Ishii, Nobuo Fukuda, Chiaki Ogino

    The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), 2010年12月, 英語, Honolulu, Hawaii, USA, 国際会議

    口頭発表(一般)

  • 麹菌A. oryzaeによる海洋細菌由来β-1,3-キシラナーゼの生産

    久田 博元, 岡﨑 文美, 石田 博樹, 荻野 千秋, 秦 洋二, 近藤 昭彦

    第10回 糸状菌分子生物学コンファレンス, 2010年11月, 日本語, 糸状菌分子生物学研究会, 東広島市, 国内会議

    ポスター発表

  • 麹菌A. oryzaeにより生産した海洋細菌由来β-1,3-キシラナーゼの酵素学的諸性質

    岡﨑 文美, 仲島 菜々実, 久田 博元, 荻野 千秋, 石田 博樹, 秦 洋二, 近藤 昭彦

    第10回 糸状菌分子生物学コンファレンス, 2010年11月, 日本語, 糸状菌分子生物学研究会, 東広島市, 国内会議

    ポスター発表

  • Selection of DNA aptamers using Atomic Force Microscopy

    OGINO Chiaki

    The 16th Symposium of Young Asian Biochemical Engineers' Communyty, 2010年11月, 英語, (社)化学工学会, 台湾桃園県, 国際会議

    口頭発表(一般)

  • Ionic liquid Pretreatment for Direct Ethanol Production from Cellulosic Material by Cellulase-displaying Arming Yeast

    OGINO Chiaki, YAMAGUCHI, Koji, NAKASHIMA Kazunori, KONDO Akihiko

    International Conference on Sustainable Biomass (ICSB) 2010, 2010年11月, 英語, 不明, Tamsui, Taiwan, 国際会議

    口頭発表(一般)

  • 放線菌を用いた様々なタンパクの大量分泌系の構築

    野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • 超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの機能解析

    岡﨑 文美, 仲島 菜々実, 久田 博元, 荻野 千秋, 石田 博樹, 秦 洋二, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • 麹菌による超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの高分泌生産

    久田 博元, 岡﨑 文美, 石田 博樹, 荻野 千秋, 秦 洋二, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • 過酸化チタンナノ粒子及び低線量X 線照射を併用した深部ガンの非侵襲的治療法の開発

    唐崎 美樹, 松井 かずさ, 佐藤 和好, 大原 智, 佐々木 良平, 田中 勉, 荻野 千秋, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • セルラーゼ発現バランス最適化酵母を用いたセルロースからのエタノール発酵

    山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その4)

    中島 一紀, 谷口 菜穂, 山口 剛示, 片平 悟史, 石田 亘広, 高橋 治雄, 田端 一英, 則武 義幸, 荻野 千秋, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その3)

    山口 剛示, 中島 一紀, 山田 亮祐, 荻野 千秋, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その2)

    石田 亘広, 片平 悟史, 中村 里沙, 田端 一英, 則武 義幸, 神谷 典穂, 中島 一紀, 荻野 千秋, 近藤 昭彦, 高橋 治雄

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • Cell SELEX 法によるヒト肝臓由来がん細胞に選択的に結合するDNA アプタマーの選抜

    仁宮 一章, 金田 壱彦, 荻野 千秋, 清水 宣明

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • Affibody 提示ナノカプセルを用いたHER2 発現癌細胞特異的な薬物送達

    三枝 宏彰, 宍戸 卓矢, 西村 勇哉, 田中 勉, 荻野 千秋, 近藤 昭彦

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    ポスター発表

  • 1細胞アッセイ技術に基づくGPCR 解析、アゴニスト探索法の開発

    近藤 昭彦, 石井 純, 荻野 千秋

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    口頭発表(一般)

  • 力学的指標による生細胞表面におけるリガンド‐受容体の相互作用解析

    野坂 和輝, 宮地 佑典, 石井 純, 荻野 千秋, 近藤 昭彦

    化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議

    ポスター発表

  • 放線菌を用いた様々なタンパクの大量分泌系の構築

    野田 修平, 田中 勉, 近藤 昭彦, 荻野 千秋

    2010年度日本放線菌学会大会, 2010年09月, 日本語, 日本放線菌学会, 東京都 江戸川区, 国内会議

    口頭発表(一般)

  • 超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質

    岡﨑 文美, 仲島 菜々実, 久田 博元, 荻野 千秋, 石田 博樹, 秦 洋二, 近藤 昭彦

    日本応用糖質科学会 平成22年度大会(第59回), 2010年09月, 日本語, 日本応用糖質科学会, 静岡市, 国内会議

    口頭発表(一般)

  • 麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの分泌高生産

    久田 博元, 岡﨑 文美, 石田 博樹, 荻野 千秋, 秦 洋二, 近藤 昭彦

    日本応用糖質科学会 平成22年度大会(第59回), 2010年09月, 日本語, 日本応用糖質科学会, 静岡市, 国内会議

    口頭発表(一般)

  • 高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発

    足立 大輔, 中島 一紀, 荻野 千秋, 近藤 昭彦

    化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議

    ポスター発表

  • 酵母によるエタノール発酵に及ぼす撹拌剪断効果

    内田 敦子, 伊藤 友教, 堀田 淳史, 堀江 孝史, 荻野 千秋, 大村 直人

    化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議

    ポスター発表

  • 機能性酵母によるバイオエタノール生産プロセスの強化

    伊藤 友教, 内田 敦子, 堀田 淳史, 堀江 孝史, 荻野 千秋, 大村 直人

    化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議

    ポスター発表

  • affibody提示バイオナノカプセルを用いたタンパク質デリバリーシステム

    西村 勇哉, 三枝 宏彰, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議

    ポスター発表

  • Construction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells

    KONDO Akihiko, HASUNUMA Tomohisa, OGINO Chiaki, TANAKA Tsutomu

    RENEWABLE ENERGY 2010, 2010年06月, 英語, International Solar Energy Society, 横浜市, 国際会議

    口頭発表(一般)

  • Intensification of Bioethanol Fermentation by Recombinant Yeast with Xylose Isomerase Pathway

    ITO, T, HOTTA, A, UCHIDA, A, TANINO, T, OGINO Chiaki, KONDO Akihiko, OHMURA Naoto

    Industrial Biotechnology International Conference 2010, 2010年04月, 英語, The Italian Association of Chemical Engineering BIOTECH WORKING GROUP, Milano, Italy, 国際会議

    口頭発表(一般)

  • 放線菌を用いた様々なタンパクの大量分泌系の構築

    野田 修平, 岡井 直子, 田中 勉, 近藤 昭彦, 荻野 千秋

    化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議

    口頭発表(一般)

  • 非侵襲性がん治療に向けた高機能性二酸化チタンナノ粒子の開発

    唐崎 美樹, 松井 かずさ, 瀬川 麻衣子, 田中 勉, 近藤 昭彦, 荻野 千秋

    化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議

    口頭発表(一般)

  • 生デンプンからの効率的な繰返し発酵を達成するアミラーゼ発現新規酵母株の創製

    山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議

    口頭発表(一般)

  • 細胞表層工学技術を用いたヘミセルロース系バイオマスからのエタノール生産

    阪本 貴俊, 蓮沼 誠久, 堀 良美, 山田 亮祐, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議

    口頭発表(一般)

  • 酵母を用いた生デンプンからの高効率な機能性ペプチド生産

    吉田 秀世, 桐山 健太郎, 荒井 章吾, 原 清敬, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いた細胞表層におけるリガンド・レセプター間相互作用測定系の構築

    野坂 和輝, 荻野 千秋, 石井 純, 宮地 佑典, 近藤 昭彦

    日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いた機能性核酸分子・アプタマーの新規選抜法の開発

    早瀬 太治, 荻野 千秋, 宮地 佑典, 近藤 昭彦

    日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議

    口頭発表(一般)

  • 機能性酵母によるイオン液体前処理セルロースからのバイオエタノール生産

    中島 一紀, 荒井 章吾, 山口 剛示, 山田 亮祐, 片平 悟史, 石田 亘広, 高橋 治雄, 荻野 千秋, 近藤 昭彦

    日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議

    口頭発表(一般)

  • バイオマスからの燃料・化学品生産を目指した細胞工場の創製

    近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 田中 勉

    日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議

    口頭発表(一般)

  • セルラーゼ表層提示酵母を用いたイオン液体前処理セルロースからの直接エタノール発酵

    中島 一紀, 荒井 章吾, 山口 剛示, 山田 亮祐, 片平 悟史, 石田 亘広, 高橋 治雄, 荻野 千秋, 近藤 昭彦

    化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議

    口頭発表(一般)

  • カクテルδインテグレーション法によるセルラーゼ発現バランス最適化酵母の創製

    山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議

    口頭発表(一般)

  • DNAアプタマーを用いた酵素活性制御系の構築

    宮地 佑典, 清水 宣明, 荻野 千秋, 近藤 昭彦

    日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議

    口頭発表(一般)

  • AFMを用いたアプタマーによるタンパク質の検出・定量方法

    網野 智一, 荻野 千秋, 宮地 佑典, 近藤 昭彦

    日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議

    口頭発表(一般)

  • セルラーゼ提示超分子複合体提示酵母によるバイオマスの効率的分解

    荻野 千秋

    第32回日本分子生物学会年会, 2009年12月, 日本語, 日本分子生物学会, 横浜市, 国内会議

    口頭発表(招待・特別)

  • Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategy

    早瀬 太治, 宮地 佑典, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Protein production for Bio-refinery strategy

    荻野 千秋, 清水 宣明, 福田 秀樹, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Production of biodiesel fuel in ionic liquids catalyzed by whole-cell biocatalysts

    中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    口頭発表(一般)

  • New SELEX strategy for screening of DNA aptamer by AFM

    宮地 佑典, 清水 宣明, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    口頭発表(一般)

  • Investigation of the interaction between GPCR and ligand by AFM equipped with bio-molecule modified cantilever

    NOSAKA K, MIYACHI Y, ISHII Jun, OGINO Chiaki, KONDO Akihiko

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Engineering of endoglucanase expression system for Corynebacterium glutamicum

    土舘 健幸, 舘野 俊博, 田中 勉, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Efficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genes

    山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Efficient homo D-lactic acid production from xylose

    吉田 渉悟, 岡野 憲司, 田中 勉, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Efficient ethanol production from xylose by mated diploid Saccharomyces cerevisiae

    角山 裕昭, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Efficient D-lactic acid production from raw starch

    新川 智, 岡野 憲司, 田中 勉, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Efficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiae

    山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Direct fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzyme

    柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domain

    田渕 聡一郎, 伊藤 純二, 足立 崇, 石田 博樹, 秦 洋二, 田中 勉, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathway

    TANINO Takanori, HOTTA Atsushi, ITO Tomonori, ISHII Jun, YAMADA Ryousuke, HASUNUMA Tomohisa, OGINO Chiaki, OHMURA Naoto

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transporters

    堀田 淳史, 谷野 孝徳, 伊藤 友数, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦, 大村 直人

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipases

    足立 大輔, 中島 一紀, 田中 勉, 荻野 千秋, 近藤 昭彦, 福田 秀樹

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Antibody-immobilized TiO2 nanoparticles for cancer therapy

    松井 かずさ, 瀬川 麻衣子, 田中 勉, 近藤 昭彦, 荻野 千秋

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • Affibody displaying bionanocapsules for HER2 specific drug delivery

    西村 勇哉, 宍戸 卓矢, 三枝 宏彰, 田中 勉, 荻野 千秋, 近藤 昭彦

    APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議

    ポスター発表

  • セルロース資源からのバイオエタノール生産技術

    荻野 千秋

    バイテクノロジー講演会, 2009年10月, 日本語, バイオテクノロジー研究会, 福井市, 国内会議

    口頭発表(招待・特別)

  • 糖代謝ネットワークを強化した酵母による混合糖からのバイオエタノール生産

    堀田 淳史, 伊藤 友教, 内田 敦子, 谷野 孝徳, 荻野 千秋, 大村 直人

    化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 広島大学, 国内会議

    口頭発表(一般)

  • 生デンプンから高効率にエタノールを生産する新規酵母の創製

    山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

    第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議

    口頭発表(一般)

  • 菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用

    中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

    第41回 化学工学会秋季大会, 2009年09月, 日本語, (社)化学工学会, 東広島市, 国内会議

    口頭発表(一般)

  • 癌細胞標的化を目指したAffibody 提示ナノカプセルの開発

    三枝 宏彰, 宍戸 卓矢, 田中 勉, 荻野 千秋, 近藤 昭彦

    第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議

    口頭発表(一般)

  • 遺伝子組換え麹菌を用いたタンパク質大量分泌生産技術の開発

    酒井 翔司, 松田 哲弥, 足立 崇, 久田 博元, 秦 洋二, 田中 勉, 荻野 千秋, 近藤 昭彦

    第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議

    口頭発表(一般)

  • ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用

    岩崎 大記, 千賀 至, 山下 貴史, 東内 雅博, 荻野 千秋, 清水 宣明, 勝田 知尚, 山地 秀樹

    化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 東広島市, 国内会議

    ポスター発表

  • Selection of DNA aptamers using atomic force microscopy

    荻野 千秋, 宮地 佑典, 早瀬 太治, 野坂 和輝, 近藤 昭彦

    Progress on Post Genome Technologies, 2009年09月, 英語, Tsinghua, Southeast, Nanjing, Northwest University, Beijing, China, 国際会議

    口頭発表(一般)

  • Porin を用いたコリネ菌新規細胞表層提示技術の開発

    畑田 一樹, 舘野 俊博, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

    第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議

    口頭発表(一般)

  • Development of environmentally benign and consolidated process for efficient production of cellulosic ethanol

    荻野 千秋

    SIM2009 Annual Meeting and Exhibition, 2009年07月, 英語, Society for Industrial Microbiology, Toronto, Canada, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 大豆イソフラボンからのイソフラボンアグリコンの効率的生産に適した新規βグルコシダーゼの開発

    荻野 千秋

    (財)不二たん白質研究振興財団第12回研究報告会, 2009年06月, 日本語, (財)不二たん白質研究振興財団, 豊中市, 国内会議

    口頭発表(一般)

  • 放線菌遺伝子組み換え系のタンパク質分泌生産への適用

    荻野 千秋, 伊藤 裕子, 宮下 徹, 仁宮 一章, 清水 宣明

    化学工学会 第75年会, 2009年03月, 日本語, 化学工学会, 神奈川県横浜市, 国内会議

    口頭発表(一般)

  • 非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発

    松井 かずさ, 荻野 千秋, 田中 勉, 福田 秀樹, 近藤 昭彦

    化学工学会 第74年会, 2009年03月, 日本語, 化学工学会, 神奈川県横浜市, 国内会議

    口頭発表(一般)

  • バイオエタノール生産を目指したスーパー酵母の創製

    近藤 昭彦, 蓮沼 誠久, 田中 勉, 荻野 千秋

    農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議

    口頭発表(一般)

  • ソフトセルロース資源からのエタノール発酵技術の開発

    荻野 千秋, 近藤 昭彦

    日本草地学会, 2009年03月, 日本語, 日本草地学会, 神奈川県藤沢市, 国内会議

    口頭発表(招待・特別)

  • キシロース資化性遺伝子組換え酵母の代謝プロファイリング解析

    WALUYO Danang, 阿部 祐一郎, 馬場 健史, 荻野 千秋, 小林 昭雄, 近藤 昭彦, 福崎 英一郎

    農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議

    ポスター発表

  • キシロースからの高効率エタノール生産を目指した宿主酵母株の検討

    角山 裕昭, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議

    ポスター発表

  • アミラーゼ発現酵母による生デンプンからの効率的な繰り返し発酵プロセスの開発

    山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議

    ポスター発表

  • TiO&2&ナノ粒子と超音波を併用した新規がん治療法構築のための基礎的検討

    仁宮 一章, 園家 史朗, 大島 周平, 荻野 千秋, 黒田 俊一, 清水 宣明

    化学工学会 第74年会, 2009年03月, 日本語, 化学工学会, 神奈川県横浜市, 国内会議

    口頭発表(一般)

  • 未来バイオ燃料生産用スーパー(CBP)微生物の開発

    近藤 昭彦, 荻野 千秋, 田中 勉

    第31回日本分子生物学会年会;第81回日本生化学会大会 合同大会, 2008年12月, 日本語, 日本分子生物学学会, 日本生化学会, 神戸市, 国内会議

    口頭発表(一般)

  • 二酸化チタンナノ粒子による活性酸素非依存的細胞死誘導

    高木 圭子, 樋口 善博, 橋井 美奈子, 荻野 千秋, 清水 宣明

    第31回日本分子生物学会年会・第82回日本生化学会大会 合同大会, 2008年12月, 日本語, 日本分子生物学学会, 日本生化学会, 神戸市, 国内会議

    口頭発表(一般)

  • 原子間力顕微鏡を用いたアプタマーの選抜

    宮地 佑典, 清水 宣明, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    若手フロンティア研究会2008, 2008年12月, 日本語, 神戸大学研究基盤センター, 神戸市, 国内会議

    ポスター発表

  • 温度応答性磁性ナノ粒子を用いた迅速かつ高効率なアフィニティー分子の選択法の確立

    福田 展雄, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    若手フロンティア研究会2008, 2008年12月, 日本語, 神戸大学研究基盤センター, 神戸市, 国内会議

    ポスター発表

  • Production of bio-fuels and chemicals from biomass by cell surface engineered yeast strains

    荻野 千秋, 近藤 昭彦

    4th International Symposium on Biocatalysis and Biotechnology, 2008年11月, 英語, International Society of Biocatalysis and Biotechnology, Taipei, Taiwan, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Methylester Synthesis By Fungi Immobilized BSPs in Ionic Liquid

    荒井 章吾, 荻野 千秋, 谷野 孝徳, 田中 勉, 福田 秀樹, 近藤 昭彦

    4th Korea-Japan Workshop on Combinatorial Bioengineering, 2008年11月, 英語, Association of Combinatorial Bioengineering, Kookmin University, Seoul, 国際会議

    ポスター発表

  • Efficient and direct ethanol production from raw starch by d-integrant polyploid fusants of Saccharomyces cerevisiae

    山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    4th Korea-Japan Workshop on Combinatorial Bioengineering, 2008年11月, 英語, Association of Combinatorial Bioengineering, Seoul, Korea, 国際会議

    ポスター発表

  • Biorefinery strategy by cell surface engineered yeast strains

    荻野 千秋, 近藤 昭彦

    4th Korea-Japan Workshop on Combinatorial Bioengineering, 2008年11月, 英語, Association of Combinatorial Bioengineering, Seoul, Korea, 国際会議

    ポスター発表

  • 特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用

    近藤 昭彦, 宍戸 卓矢, 田中 勉, 荻野 千秋

    第17回日本バイオイメージング学会, 2008年10月, 日本語, 日本バイオイメージング学会, 千葉市, 国内会議

    口頭発表(一般)

  • Improvement of Yeast Xylose Uptake Ability for Efficient Bioethanol Production

    TANINO Takanori, OGINO Chiaki, KUMAGAI Norihisa, OHMURA Naoto

    International Workshop on Process Intensification 2008, 2008年10月, 英語, 化学工学会粒子流体プロセス部会, 化学工学会システム・情報・シミュレーション部会, 化学工学会反応工学部会, 化学工学会マイクロ化学研究会, 東京工業大学, 国際会議

    ポスター発表

  • セルラーゼ発現酵母によるセルロースからのバイオエタノール生産

    柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    化学工学会 第40回 秋季大会, 2008年09月, 日本語, 化学工学会, 宮城県仙台市, 国内会議

    ポスター発表

  • 無蒸煮デンプンから効率的にエタノールを生産する高機能酵母の創製

    山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    第60回 日本生物工学会2008年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議

    口頭発表(一般)

  • 遺伝子組み換え放線菌を用いたセルラーゼ発現システムの構築

    伊藤 裕子, 宮下 徹, 清水 宣明, 荻野 千秋

    第60回 日本生物工学会2010年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素のイオン液体中での反応解析

    宮下 徹, 伊藤 裕子, 清水 宣明, 荻野 千秋

    第60回 日本生物工学会2009年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議

    口頭発表(一般)

  • キチン結合ドメインを用いた麹菌細胞表層提示技術の開発

    田淵 聡一郎, 伊藤 純二, 足立 崇, 石田 博樹, 秦 洋二, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    第60回 日本生物工学会2008年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議

    口頭発表(一般)

  • Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼC の遺伝子クローニングと発現

    杉森 大助, 冨田 優, 荻野 千秋, 劉 暁麗

    第60回 日本生物工学会2008年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議

    口頭発表(一般)

  • Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen species

    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Daiki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

    American Society for Microbiology 108th General Meeting, 2008年06月, 英語, American Society for Microbiology, Boston, USA, 国際会議

    ポスター発表

  • フローサイトメーター(FCM)を利用した酵母GPCRシグナリング解析技術

    石井 純, 田中 勉, 松村 静香, 立松 健司, 黒田 俊一, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    化学工学会第73年会, 2008年03月, 日本語, 化学工学会, 浜松市, 国内会議

    口頭発表(一般)

  • 酵母GPCRシグナリングにおけるフローサイトメトリー解析技術

    石井 純, 田中 勉, 松村 静香, 立松 健司, 黒田 俊一, 荻野 千秋, 福田 秀樹, 近藤 昭彦

    第6回最先端バイオテクノロジー若手発表会, 2008年02月, 日本語, 化学工学会関西支部化学工学会バイオ部会, 西宮市, 国内会議

    ポスター発表

  • ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化

    千賀 至, 山神 啓輔, 勝田 知尚, 萩野 千秋, 清水 宣明, 加藤 滋雄

    第6回 最先端バイオテクノロジー公開セミナー, 2008年02月, 日本語, 化学工学会関西支部、化学工学会バイオ部会, 西宮, 国内会議

    口頭発表(一般)

  • Ultrasonic facilitation of OH radical generation in the presence of TiO2

    SHIMIZU N, 荻野 千秋, DADJOUR M.F

    ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議

    口頭発表(一般)

  • The cancer cell injury by combination of protein displayed titanium dioxide nanoparticle and ultrasound irradiation

    SASAI R, SONOKE S, 荻野 千秋, KURODA S, SHIMIZU N

    ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議

    口頭発表(一般)

  • Construction of biomolecule-immobilized TiO2 nanoparticle for applying to new cell injuring method with ultrasound irradiation

    荻野 千秋, SASAI R, SONOKE S, KURODA S, SHIMIZU N

    ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議

    口頭発表(一般)

  • Analysis of Sonodynamic Sterilization of Escherichia coli at the Surface of TiO2

    RAHMAN M.M, 荻野 千秋, SHIMIZU N

    ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議

    口頭発表(一般)

  • Analysis of H2O2 formation induced by ultrasonic irradiation with TiO2. K

    SAKIYAMA K, ONIWA T, 荻野 千秋, SHIMIZU N

    ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議

    口頭発表(一般)

  • Oxidative treatment of H. pluvialis with the sonocatalytic reaction

    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Hiroki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

    YABEC2007, 2007年10月, 英語, Young Asian Biochemical Engineers Community, Seoul, Korea, 国際会議

    ポスター発表

  • Application of biomolecule-immobilized TiO2 nanoparticle for chemical compound degradation and mammalian cell killing

    荻野 千秋, SASAI R, SONOKE S, SHIMIZU N

    YABEC2007, 2007年10月, 英語, YABEC, Souel 韓国, 国際会議

    口頭発表(一般)

  • 放線菌を宿主とするリン脂質代謝酵素の変異体解析

    伊藤 吉基, 宮下 徹, 荻野 千秋, 清水 宣明

    日本生物工学会第59回大会, 2007年09月, 日本語, 日本生物工学会, 東広島, 国内会議

    口頭発表(一般)

  • 放線菌Streptomyces lividans における分泌シグナルのタンパク生産に及ぼす影響解析

    吉野 慶和, 伊藤 裕子, 荻野 千秋, 清水 宣明

    日本生物工学会第59回大会, 2007年09月, 日本語, 日本生物工学会, 東広島, 国内会議

    口頭発表(一般)

  • 二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導

    勝田 知尚, 荻野 千秋, 山神 啓輔, 加藤 滋雄, 清水 宣明

    第59回生物工学会大会, 2007年09月, 日本語, 日本生物工学会, 広島, 国内会議

    口頭発表(一般)

  • Over-expression system of secretory phospholipase D by Streptomyces lividans

    荻野 千秋, KUBO T, YOSHINO K, SHIMIZU N

    ECB13, 2007年09月, 英語, ECB, バルセロナ スペイン, 国際会議

    口頭発表(一般)

  • New SELEX strategy for screening of DNA aptamer by AFM.

    荻野 千秋, MIYACHI Y, SHIMIZU N

    The 2nd International Workshop on Approaches to Single-Cell Analysis, 2007年09月, 英語, 東京, 国際会議

    口頭発表(一般)

  • Induction of astaxantin biosynthesis in H. pluvialis using TiO2 and ultrasounds

    勝田 知尚, 荻野 千秋, YAMAGAMI K, KATOH S, SHIMIZU N

    ECB13, 2007年09月, 英語, ECB, バルセロナ スペイン, 国際会議

    口頭発表(一般)

  • Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasounds

    KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

    13th European Congress on Biotechnology, 2007年09月, 英語, European Federation of Biotechnology, Barcelona, Spain, 国際会議

    ポスター発表

  • Phospholipid conversion in ionic liquid by phospholipase D

    荻野 千秋, MIYASHITA T, SHIMIZU N

    2nd International Congress on Ionic Liquids (COIL-2), 2007年08月, 英語, 横浜, 国際会議

    口頭発表(一般)

  • 放線菌の新規タンパク質発現系構築に関する研究

    久保 孝文, 吉野 和慶, 伊藤 吉基, 宮下 徹, 荻野 千秋, 清水 宣明

    化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議

    口頭発表(一般)

  • 組織ターゲティング機能を有する二酸化チタンナノ粒子によるがん細胞障害

    柴田 直範, 笹井 良祐, 高木 圭子, 黒田 俊一, 荻野 千秋, 清水 宣明

    化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素の酵素活性に及ぼすイオン液体の効果

    荻野 千秋, 伊藤 吉基, 宮下 徹, 清水 宣明

    日本化学会第87春季年会, 2007年03月, 日本語, 日本化学会, 吹田, 国内会議

    口頭発表(一般)

  • 8OH-dGを認識するDNAアプタマーの機能評価

    宮地 佑典, 伊藤 裕子, 荻野 千秋, 清水 宣明

    日本化学会第87春季年会, 2007年03月, 日本語, 日本化学会, 吹田, 国内会議

    口頭発表(一般)

  • Structural analysis of 8OH-dG DNA aptamer

    荻野 千秋, MIYACHI Y, ITOU Y, SHIMIZU N

    9th Japan-China-Korea Joint symposium on Enzyme Engineering, 2006年11月, 英語, 酵素工学研究会, 大津, 国際会議

    口頭発表(一般)

  • Over-expression system for secretory enzyme by Streptomyces lividans

    荻野 千秋, KUBO T, YOSHINO K, ITOU Y, MIYASHITA T, SHIMIZU N

    YABEC2006, 2006年11月, 英語, YABEC, 高雄, 国際会議

    口頭発表(一般)

  • Effect of solvent and ionic liquid on phospholipid conversion in bi-phase system by phospholipase D

    ITOU Y, KUBO T, YOSHINO K, MIYASHITA T, 荻野 千秋, SHIMIZU N

    9th Japan-China-Korea Joint symposium on Enzyme Engineering, 2006年11月, 英語, 酵素工学研究会, 大津, 国際会議

    口頭発表(一般)

  • Construction of secretional expression system by Streptomyces lividans

    YOSHINO T, KUBO T, ITOU Y, MIYASHITA T, 荻野 千秋, SHIMIZU N

    9th Japan-China-Korea Joint symposium on Enzyme Engineering, 2006年11月, 英語, 酵素工学研究会, 大津, 国際会議

    口頭発表(一般)

  • 二酸化チタン超音波触媒法を応用した有害微生物殺菌システム

    MIZANUR R.M, 荻野 千秋, 清水 宣明

    第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議

    口頭発表(一般)

  • 二酸化チタン超音波触媒法による細胞死誘導

    高木 圭子, 荻野 千秋, 清水 宣明

    第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議

    口頭発表(一般)

  • 二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果

    藤平 敦司, 荻野 千秋, 清水 宣明

    第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議

    口頭発表(一般)

  • 二酸化チタンへの超音波照射による過酸化水素生成機構の解析

    崎山 和亨, 荻野 千秋, 清水 宣明

    第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議

    口頭発表(一般)

  • 放線菌を用いたホスホリパーゼD酵素の遺伝子組み換え生産の培養特性解析に関する研究

    久保 孝文, 吉野 和慶, 伊藤 吉基, 宮下 徹, 荻野 千秋, 清水 宣明

    日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議

    口頭発表(一般)

  • 二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果

    藤平 敦司, 崎山 和享, 荻野 千秋, 清水 宣明

    化学工学会第38回秋季大会, 2006年09月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • 二酸化チタン・超音波触媒法によるがん細胞障害と増殖抑制

    高木 圭子, 荻野 千秋, 清水 宣明

    日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議

    口頭発表(一般)

  • 組織認識能を持つ二酸化チタン内包リポソームの創製

    荻野 千秋, 高木 圭子, 清水 宣明

    日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議

    口頭発表(一般)

  • 生体ストレスマーカー分子8OH-dGを認識するDNAアプタマーに関する研究

    宮地 佑典, 伊藤 裕子, 荻野 千秋, 清水 宣明

    日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議

    口頭発表(一般)

  • 8OH-dGを認識するDNAアプタマーに関する基礎研究

    宮地 祐介, 荻野 千秋, 清水 宣明

    化学工学会第38回秋季大会, 2006年09月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • 二酸化チタン・超音波触媒法によるがん細胞傷害と増殖抑制

    山岸 紗弥花, 高木 圭子, FARSHBAF M.D, 荻野 千秋, 清水 宣明

    化学工学会第71年会, 2006年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 組織認識能をもつ二酸化チタン内包リポソームの創製

    野瀬 律子, 高木 圭子, FARSHBAF M.D, 中川 晋作, 荻野 千秋, 清水 宣明

    化学工学会第71年会, 2006年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素の蓋構造変化における活性相関に関する研究(II)

    大石 多佳子, 伊藤 吉基, 高田 奈充子, 荻野 千秋, 清水 宣明

    化学工学会第71年会, 2006年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 放線菌Streptomyces cinnamoneum由来発現プロモーターを用いたタンパク質発現系構築に関する研究

    久保 孝文, 吉野 和慶, 荻野 千秋, 清水 宣明

    日本生物工学会第57回大会, 2005年11月, 日本語, 日本生物工学会, 筑波, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素ホスホリパーゼDの蓋構造の活性相関に関する研究

    大石 多佳子, 高田 奈充子, 伊藤 吉基, 荻野 千秋, 清水 宣明

    日本生物工学会第57回大会, 2005年11月, 日本語, 日本生物工学会, 筑波, 国内会議

    口頭発表(一般)

  • 有害微生物の超音波殺菌に対する二酸化チタンの増強効果

    FARSHBAF D.M, 荻野 千秋, 清水 宣明

    第14回ソノケミストリー討論会, 2005年10月, 日本語, ソノケミストリー学会, 豊橋, 国内会議

    口頭発表(一般)

  • 放線菌を宿主とするタンパク質発現系の構築

    福本将士, 荻野 千秋, 清水宣明

    化学工学会第70年会, 2005年03月, 日本語, 化学工学会, 名古屋, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素の蓋構造変化における活性相関に関する研究

    泉 麻里子, 荻野 千秋, 清水 宣明

    化学工学会第70年会, 2005年03月, 日本語, 化学工学会, 名古屋, 国内会議

    口頭発表(一般)

  • Screening of DNA aptamer that recognizes nucleotide derivative

    荻野 千秋, MURATA T, MIYACHI Y, SHIMIZU N

    Bioseparation2004, 2004年10月, 英語, 化学工学会, 福岡, 国際会議

    口頭発表(一般)

  • Investigation of the Catalytic Function of Phospholipase D by Site Directed Mutagenesis

    荻野 千秋, IZUMI M, OHISHI T, FUKUMOTO M, SHIMIZU N

    10thAPCChE, 2004年10月, 英語, 化学工学会, 北九州, 国際会議

    口頭発表(一般)

  • Cell Disinfection by Ultrasonic Irradiation with TiO2 Photocatalyst

    FARSHBAF D.M, 荻野 千秋, MATSUMURA S, NAKAMURA S, SHIMIZU N

    10thAPCChE, 2004年10月, 英語, 化学工学会, 北九州, 国際会議

    口頭発表(一般)

  • 低分子化合物を認識するDNAアプタマーの取得とそのバイオセンサーへの応用

    荻野 千秋, 村田知之, 宮地佑典, 清水宣明

    化学工学会山口下関大会, 2004年09月, 日本語, 化学工学会, 下関, 国内会議

    口頭発表(一般)

  • 生理活性物質を認識するDNAアプタマーの探索とその機能評価

    荻野 千秋, 村田知之, 清水宣明

    日本生物工学会第56回大会, 2004年09月, 日本語, 日本生物工学会, 名古屋, 国内会議

    口頭発表(一般)

  • Screening of DNA aptamer that recognizes nucleotide derivative

    荻野 千秋, MURATA T, MIYACHI Y, SHIMIZU N

    YABEC2004, 2004年09月, 英語, YABEC, 大阪, 国際会議

    口頭発表(一般)

  • 有機溶媒感受性プロモーターを用いた水質評価法の構築

    伊藤 武男, 荻野 千秋, 森本 真壽, 清水 宣明

    化学工学会第69年会, 2004年04月, 日本語, 化学工学会, 堺, 国内会議

    口頭発表(一般)

  • Investigation of catalytic function of phospholipase D enzyme by site directed mutagenesis

    荻野 千秋, IZUMI M, OISHI T, SHIMIZU N

    1st PRICPS, 2004年04月, 英語, 日本蛋白質学会, 横浜, 国際会議

    口頭発表(一般)

  • Disinfection of Legionella by sonocatalytic irradiation with TiO2 photocatalyst

    FARSHBAF D.M, 松村享, 荻野 千秋, 清水宣明

    化学工学会第69年会, 2004年04月, 英語, 化学工学会, 堺, 国内会議

    口頭発表(一般)

  • 新規ラジカル生成法の環境浄化と医療への応用

    清水 宣明, 荻野 千秋

    化学工学会京都大会, 2003年12月, 日本語, 化学工学会, 京都, 国内会議

    口頭発表(一般)

  • Screening of DNA aptamer that recognizes phenol molecule.

    荻野 千秋, MURATA T, MIYAMOTO N, KANEHIRA K, SHIMIZU N

    YABEC2003, 2003年11月, 英語, YABEC, 済洲島, 国際会議

    口頭発表(一般)

  • 二酸化チタン・超音波照射法による大腸菌殺菌メカニズムの解析

    FARSHBAF D.M, 松村 享, 荻野 千秋, 清水 宣明

    第12回ソノケミストリー討論会, 2003年10月, 日本語, ソノケミストリー学会, 福岡, 国内会議

    口頭発表(一般)

  • 放線菌由来リン脂質代謝酵素の変異体の機能解析

    荻野 千秋, 泉 麻里子, 大石 多佳子, 清水 宣明

    日本生物工学会第55回大会, 2003年09月, 日本語, 日本生物工学会, 熊本, 国内会議

    口頭発表(一般)

  • Investigation of catalytic function of phospholipase D enzyme by site directed mutagenesis

    荻野 千秋, IZUMI M, SHIMIZU N

    European Conference of Biotechnology (ECB) 11th, 2003年08月, 英語, ECB, バーゼル・スイス, 国際会議

    口頭発表(一般)

  • 分子進化法を用いたPLDの触媒機能変換

    畑中 祐樹, 泉 麻里子, 荻野 千秋, 清水 宣明

    化学工学会第68年会, 2003年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 機能性DNA分子の探索と水質評価への応用

    金平 幸輝, 村田 知之, 荻野 千秋, 清水 宣明

    化学工学会第68年会, 2003年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • Bio-sensing of benzene derivatives using Ps promoter gene and GFP as a reporter protein (II)

    荻野 千秋, IKENO S, ITO T, SHIMIZU N

    YABEC2002, 2002年11月, 英語, YABEC, 台北, 国際会議

    口頭発表(一般)

  • 有機溶媒感受性プロモーターを用いた水質評価法の構築

    池野 慎也, 伊藤 武男, 杉野 康弘, 荻野 千秋, 清水 宣明

    日本生物工学会第54回大会, 2002年10月, 日本語, 日本生物工学会, 大阪, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素ホスホリパーゼDの遺伝子工学的手法による触媒機能解析

    荻野 千秋, 畑中 祐樹, 泉 麻里子, 清水 宣明

    日本生物工学会第54回大会, 2002年10月, 日本語, 日本生物工学会, 大阪, 国内会議

    口頭発表(一般)

  • 有機化合物認識遺伝子群を用いた水質評価法の構築

    池野 慎也, 伊藤 武男, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議

    口頭発表(一般)

  • 変異型ホスホリパーゼDを用いた新規リン脂質合成への検討

    畑中 祐樹, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議

    口頭発表(一般)

  • 二酸化チタン界面での超音波力学的殺菌メカニズムの解析

    中村 淳, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議

    口頭発表(一般)

  • 二酸化チタン・超音波法による内分泌撹乱物質の分解処理に関する研究

    前川 和人, 川西 琢也, 林 良茂, 荻野 千秋, 清水 宣明

    化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議

    口頭発表(一般)

  • 有機溶媒感受性プロモーターを用いた水質評価法の構築

    池野 慎也, 伊藤 武男, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第67年会, 2002年03月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • 二酸化チタン超音波照射法によるヒドロキシラジカルの生成と内分泌撹乱物質の分解処理

    島田 泰行, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第67年会, 2002年03月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • ホスホリパーゼDにおける構造変化とリン脂質合成相関に関する解析

    荻野 千秋, 大村 有香, 畑中 祐樹, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第67年会, 2002年03月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • Construction of functional phospholipid synthesis system by using mutant phospholipase D enzyme

    荻野 千秋, OHMURA Y, HATANAKA Y, KISHI N, SHIMIZU N

    YABEC2001, 2001年10月, 英語, YABEC, 上海, 国際会議

    口頭発表(一般)

  • Construction of benzene derivatives biosensor using Ps promoter gene and GFP as a reporter gene

    IKENO S, 荻野 千秋, ITO T, SHIMIZU N

    YABEC2001, 2001年10月, 英語, YABEC, 上海, 国際会議

    口頭発表(一般)

  • ホスホリパーゼDの触媒機構解析と新規リン脂質合成への応用

    荻野 千秋, 大村 有香, 畑中 祐樹, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会福井大会, 2001年07月, 日本語, 化学工学会, 福井, 国内会議

    口頭発表(一般)

  • レポーター遺伝子組換え微生物を用いる水質評価法の構築

    池野 慎也, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第66年会, 2001年04月, 日本語, 化学工学会, 東広島, 国内会議

    口頭発表(一般)

  • リン脂質代謝酵素ホスホリパーゼD の触媒機構及び活性化機構の解析

    大村 有香, 畑中 祐樹, 荻野 千秋, 川西 琢也, 林 良茂, 清水 宣明

    化学工学会第66年会, 2001年04月, 日本語, 化学工学会, 東広島, 国内会議

    口頭発表(一般)

  • Improved catalytic properties of phospholipase D by site directed mutagenesis

    荻野 千秋, OMURA Y, 近藤 昭彦, SHIMIZU N, 福田 秀樹

    YABEC2000, 2000年11月, 英語, YABEC, 福岡, 国際会議

    口頭発表(一般)

  • Secretory Overexpression of Phospholipase D By Recombinant Streptomyces lividans

    KANEMASU M, 荻野 千秋, DAIDO H, HAYASHI Y, 近藤 昭彦, 福田 秀樹, KURODA S

    化学工学会秋季大会, 2000年09月, 英語, 化学工学会, 浜松, 国内会議

    口頭発表(一般)

  • 遺伝子工学的手法による活性変異型ホスホリパーゼDの構築と解析

    荻野 千秋, 大村 有香, 清水 宣明, 金升 将征, 近藤 昭彦, 福田 秀樹

    日本生物工学会第52回大会, 2000年08月, 日本語, 日本生物工学会, 札幌, 国内会議

    口頭発表(一般)

  • 放線菌Stv. cinnamoneum由来PLDの発現系構築と触媒機構解析

    大同 英則, 荻野 千秋, 金升 将征, 近藤 昭彦, 福田 秀樹

    日本生物工学会第51回大会, 1999年09月, 日本語, 日本生物工学会, 吹田市, 国内会議

    口頭発表(一般)

  • メタボローム解析が明らかにする低酸素濃度におけるコリネ型細菌の芳香族化合物代謝促進機構と代謝改変によるソルガム搾汁液からの物質生産

    川口 秀夫, 宮崎 翔子, 蓮沼 誠久, 荻野 千秋, 佐塚 隆志, 近藤 昭彦

    日本農芸化学会2020年度大会

共同研究・競争的資金等の研究課題

  • 過酸化チタンナノ粒子を用いたTheranostic粒子線治療の開発

    赤坂 浩亮

    学術研究助成基金助成金/基盤研究(C), 2019年04月 - 2022年03月

    競争的資金

  • 【JST-SATREPS】インドネシアにおける統合バイオリファイナリーシステムの開発

    荻野 千秋

    国立研究開発法人科学技術振興機構, 国際科学技術共同研究推進事業・地球規模課題対応国際科学技術協力プログラム(SATREPSインドネシア), 2017年11月 - 2018年03月, 研究代表者

    競争的資金

  • 岡田 卓也

    学術研究助成基金助成金/基盤研究(C), 2017年04月 - 2020年03月

    競争的資金

  • 【ALCA】イオン液体前処理バイオマスの酵素糖化、および得られる糖液の特性評価

    荻野 千秋

    国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(先端的低炭素化技術開発ALCA), 2017年, 研究代表者

    競争的資金

  • 荻野 千秋

    科学研究費補助金/基盤研究(B), 2016年04月 - 2019年03月, 研究代表者

    競争的資金

  • 佐々木 良平

    学術研究助成基金助成金/挑戦的萌芽研究, 2016年04月 - 2019年03月

    競争的資金

  • 酵素法による植物油からのBDFと副産物バイオマスへの変換(基礎条件検討)

    荻野 千秋

    戦略的イノベーション創造プログラム(SIP), 2016年, 研究代表者

    競争的資金

  • 【JST-SATREPS】インドネシアにおける統合バイオリファイナリーシステムの開発

    荻野 千秋

    国際科学技術共同研究推進事業・地球規模課題対応国際科学技術協力プログラム(SATREPSインドネシア), 2016年, 研究代表者

    競争的資金

  • 【CREST】植物バイオマスを原料とした芳香族ポリマー原料の発酵生産

    荻野 千秋

    国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(CREST), 2016年, 研究代表者

    競争的資金

  • 【ALCA】イオン液体前処理バイオマスの酵素糖化、および得られる糖液の特性評価

    荻野 千秋

    国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(ALCA), 2016年, 研究代表者

    競争的資金

  • SATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」

    荻野 千秋

    地球規模課題対応国際科学技術協力プログラム, 2015年, 研究代表者

    競争的資金

  • SATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」

    荻野 千秋

    地球規模課題対応国際科学技術協力プログラム, 2014年, 研究代表者

    競争的資金

  • 荻野 千秋

    学術研究助成基金助成金/基盤研究(C), 2013年04月 - 2016年03月, 研究代表者

    競争的資金

  • 佐々木 良平

    学術研究助成基金助成金/挑戦的萌芽研究, 2013年04月 - 2016年03月

    競争的資金

  • 【CREST】植物バイオマスを原料とした芳香族ポリマー原料の発酵生産

    荻野 千秋

    国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(CREST), 2013年 - 2018年, 研究代表者

    競争的資金

  • 二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」

    荻野 千秋

    二国間交流事業(インドネシアとの共同研究), 2013年, 研究代表者

    競争的資金

  • SATREPS「統合バイオリファイナリー研究拠点構築プロジェクト(インドネシア)」

    荻野 千秋

    地球規模課題対応国際科学技術協力プログラム, 2013年, 研究代表者

    競争的資金

  • 近藤 昭彦

    科学研究費補助金/基盤研究(B), 2012年04月 - 2015年03月

    競争的資金

  • 福田 秀樹

    科学研究費補助金/基盤研究(B), 2012年04月 - 2015年03月

    競争的資金

  • 二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」

    荻野 千秋

    二国間交流事業(インドネシアとの共同研究), 2012年, 研究代表者

    競争的資金

  • イオン液体を利用したバイオマスからのバイオ燃料生産技術の開発

    荻野 千秋

    独立行政法人新エネルギー・産業技術総合開発機構, バイオマスエネルギー等高効率転換技術開発, 2012年, 研究代表者

    競争的資金

  • SATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」

    荻野 千秋

    地球規模課題対応国際科学技術協力プログラム, 2012年, 研究代表者

    競争的資金

  • 二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」

    荻野 千秋

    二国間交流事業(インドネシアとの共同研究), 2011年, 研究代表者

    競争的資金

  • 近藤 昭彦

    学術研究助成基金助成金/挑戦的萌芽研究, 2011年

    競争的資金

  • イオン液体を利用したバイオマスからのバイオ燃料生産技術の開発

    荻野 千秋

    独立行政法人新エネルギー・産業技術総合開発機構, バイオマスエネルギー等高効率転換技術開発, 2011年, 研究代表者

    競争的資金

  • 産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」

    荻野 千秋

    2010年, 研究代表者

    競争的資金

  • 荻野 千秋

    科学研究費補助金/若手研究(B), 2009年, 研究代表者

    競争的資金

  • 産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」

    荻野 千秋

    2009年, 研究代表者

    競争的資金

  • 産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」(2)

    荻野 千秋

    2008年, 研究代表者

    競争的資金

  • 産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」(1)

    荻野 千秋

    2008年, 研究代表者

    競争的資金

  • 荻野 千秋

    科学研究費補助金/若手研究(B), 2007年, 研究代表者

    競争的資金

  • 放射菌によるP450系酵素群の大量発現系の開発

    荻野 千秋

    2007年, 研究代表者

    競争的資金

  • 荻野 千秋

    科学研究費補助金/特定領域研究, 2007年, 研究代表者

    競争的資金

  • 荻野 千秋

    科学研究費補助金/特定領域研究, 2007年, 研究代表者

    競争的資金

産業財産権

  • 糖液の製造方法及び多糖類系バイオマス由来化合物の製造方法

    佐々木 建吾, 荻野 千秋, 近藤 昭彦

    特願2014-129051, 2014年06月24日, 大学長, 特許6403188, 2018年09月21日

    特許権

  • βグルコシダーゼを発現する大腸菌

    近藤 昭彦, 荻野 千秋, 田中 勉

    特願2010-258361, 2010年11月18日, 大学長, 特許5846523, 2015年12月04日

    特許権

  • 放射線治療剤

    荻野 千秋, 田中 勉, 佐々木 良平, 近藤 昭彦

    特願2012-500638, 2011年02月17日, 大学長, 特許5649137, 2014年11月21日

    特許権

  • 放射線治療剤(中)

    荻野 千秋, 田中 勉, 佐々木 良平, 近藤 昭彦

    201180008541.3, 2011年02月17日, 大学長, ZL201180008541.3, 2014年07月02日

    特許権

  • 放射線治療剤

    荻野 千秋, 田中 勉, 佐々木 良平, 近藤 昭彦

    13/578836, 2011年02月17日, 大学長, 8580312, 2013年11月12日

    特許権

  • セルロース含有材料からの有用物質の生産方法

    近藤 昭彦, 荻野 千秋

    特願2009-183880, 2009年08月06日, 大学長, 特許5120965, 2012年11月02日

    特許権