研究者紹介システム
| 藤嶽 暢英 |  |
| フジタケ ノブヒデ | |
| 大学院農学研究科 生命機能科学専攻 | |
| 教授 | |
| 農芸化学関係 |
研究者情報
所属
【主配置】
大学院農学研究科 生命機能科学専攻【配置】
農学部 生命機能科学科, 附属図書館
学位
授業科目
- 根圏土壌化学, 大学院農学研究科, 2021
- 特定課題演習 I-1, 大学院農学研究科, 2021
- 課題開発演習, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅰ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-1, 大学院農学研究科, 2021
- 特定課題演習 I-2, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 土壌機能解析学, 大学院農学研究科, 2021
- 生態・環境制御論Ａ, 大学院農学研究科, 2021
- 生態・環境制御論Ｂ, 大学院農学研究科, 2021
- 高度教養セミナー農学部環境生物学入門, 農学部, 2021
- 高度教養セミナー農学部環境生物学入門, 農学部, 2021
- 高度教養セミナー農学部環境生物学, 農学部, 2021
- 卒業研究, 農学部, 2021
- バイオサイエンス基礎英語１, 農学部, 2021
- 環境生物学実験II, 農学部, 2021
- 基礎土壌学, 農学部, 2021
- 生物科学英語リスニング演習, 農学部, 2021
- バイオサイエンス基礎英語２, 農学部, 2021
- 土壌鉱物学, 農学部, 2021
- 緑の保全, 農学部, 2021
- 緑の保全の探究, 農学部, 2021
- 生命機能科学各論, 農学部, 2021
- 生命機能科学各論II, 農学部, 2021
- 環境生物学実験I, 農学部, 2021
- 環境生物学実験III, 農学部, 2021
ミニ講義
ジャンル
コメントテーマ
研究活動
研究分野
受賞
2020年05月 日本土壌肥料学会, 学会賞, 腐植物質の分析法，特徴付けおよび反応性に関する研究
2017年11月 日本腐植物質学会, 日本腐植物質学会第33回講演会優秀ポスター発表賞, 南極湖沼溶存有機物に光を当てる：光学的分析およびフミン物質定量
日本国国内学会・会議・シンポジウム等の賞
2017年03月 日本生態学会, 第６４回⽇日本⽣生態学会⼤大会ポスター賞, 亜熱帯マングローブ林林における粗⼤大有機物 の分解速度度と環境要因の関係
日本国国内学会・会議・シンポジウム等の賞
2016年09月 日本土壌肥料学会, ポスター賞, チベット高原祁連（きれん）山脈における標高別土壌の有機物特性
日本国国内学会・会議・シンポジウム等の賞
2016年09月 International Humic Substances Society, The Award for the Best Poster, Determination of aquatic humic substances in lakes and wetlands by the carbon concentration-based DAX-8 batch adsorption technique.
日本国国際学会・会議・シンポジウム等の賞
2014年10月 独立行政法人 日本学術振興会, 平成26年度「科研費」審査員表彰, 平成26年度「科研費」審査員
有意義な審査意見を付し，公正・公平な審査に大きく貢献したため, 日本国その他の賞
2014年02月 日本陸水学会近畿支部会, 日本陸水学会近畿支部会第25回研究発表会 優秀発表賞, 国内外の湖沼や河川における腐植物質含量～DAX-8 樹脂吸着法による〜
口頭発表において優秀な成績を収めた, 日本国国内学会・会議・シンポジウム等の賞
論文
- 2021年12月, Pedologist, 65 (1), 英語Soil properties and classification of a small-scale steep sloping field under traditional slash and burn cropping systems in Sabah, Malaysia.
[査読有り]
研究論文（学術雑誌）
- 2021年11月, Humic Substances Research, 18, 1 - 6, 日本語腐植物質に関する近年の論争について：腐植物質パラダイムの行方
[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2021年08月, Science of The Total Environment, 149682 - 149682, 英語
[査読有り]
研究論文（学術雑誌）
- American Chemical Society (ACS), 2021年07月20日, Environmental Science & Technology, 55 (14), 10175 - 10185, 英語
[査読有り]
研究論文（学術雑誌）
- 2020年11月, 土と微生物, 74 (2), 54 - 59, 日本語土壌の炭素循環と気候変動
[査読有り][招待有り]
研究論文（学術雑誌）
- 2020年10月, 日本土壌肥料学雑誌, 91 (5), 317 - 320腐植物質の分析法，特徴付けおよび反応性に関する研究
[査読有り]
研究論文（学術雑誌）
Despite the recognized organic carbon (OC) sequestration potential of mangrove forests, the ongoing climate change and anthropogenic disturbances pose a great threat to these ecosystems. However, we currently lack the ability to mechanically understand and predict the consequences of such impacts, primarily because mechanisms underlying OC stabilization in these ecosystems remain elusive. Research into OC stabilization has focused on terrestrial soils and marine sediments for decades, overlooking the vegetated coastal ecosystems including mangroves. In terrestrial soils and marine sediments, it is widely accepted that OC stabilization is the integrated consequence of OM's inherent recalcitrance, physical protection, and interactions with minerals and metals. However, related discussion is rarely done in mangrove soils, and recalcitrance of roots and high net ecosystem production (high primary production and low heterotrophic respiration) have been considered as a primary OC sequestration mechanism in mangrove peat and mineral soils, respectively. This review presents the available information on the mechanisms underlying OC stabilization in mangrove soils and highlights research questions that warrant further investigation. Primary OC stabilization mechanisms differ between mangrove peat and mineral soils. In mangrove mineral soils, physico-chemical stabilization processes are important, yet grossly understudied OC stabilization mechanisms. In mangrove peat, recalcitrance of mangrove roots and the inhibition of phenoloxidase under the anoxic condition may be the primary OC stabilization mechanisms. Salinity-induced OC immobilization likely plays a role in both type of soils. Finally, this review argues that belowground production and allochthonous inputs in mangrove forests are likely underestimated. More studies are needed to constrain C budgets to explain the enigma that mangrove OC keeps accumulating despite much higher decomposition (especially by large lateral exports) than previously considered.
{MDPI} {AG}, 2020年09月11日, Forests, 11 (9), 981 - 981, 英語[査読有り]
研究論文（学術雑誌）
The significance of aquatic lateral carbon (C) export in mangrove ecosystems highlights the extensive contribution of aquatic pathways to the net ecosystem carbon budget. However, few studies have investigated lateral fluxes of dissolved organic carbon (DOC) and inorganic carbon (DIC), partly due to methodological difficulty. Therefore, we evaluated area-based lateral C fluxes in a small mangrove estuary that only had one exit for water exchange to the coast. We sampled water from the mouth of the creek and integrated discharge and consecutive concentration of mangrove-derived C (ΔC). Then, we estimated the area-normalized C fluxes based on the inundated mangrove area. DIC and DOC concentrations at the river mouth increased during ebb tide during both summer and winter. We quantified the ΔC in the estuary using a two-component conservative mixing model of freshwater and seawater. DIC and DOC proportions of ΔC concentrations at the river mouth during ebb tide was between 34% and 56% in the winter and 26% and 42% in the summer, respectively. DIC and DOC fluxes from the estuary were estimated to be 1.36 g C m−2 d−1 and 0.20 g C m−2 d−1 in the winter and 3.35 g C m−2 d−1 and 0.86 g C m−2 d−1 in the summer, respectively. Based on our method, daily fluxes are mangrove area-based DIC and DOC lateral exports that can be directly incorporated into the mangrove carbon budget.
MDPI AG, 2020年09月, Forests, 11 (10), 1041 - 1041[査読有り]
研究論文（学術雑誌）
Organic matter in marine sediment is mainly categorized into three fractions depending on degradability: labile, semi labile and refractory. The degradability of coastal marine sediments depends on the properties of the organic matter contained in the sediments. The purpose of this study was to quantify labile and refractory organic matter in coastal marine sediments with different characteristics collected from the Seto Inland Sea, and to discover the factors controlling the degradability of organic matter. Refractory organic matter content in sediments ranged from 4.7-21.4 mg g(-1), a percent composition equivalent to 72-97% of TOC. In contrast, labile and semi-labile content were 0.1-1.4 mg g(-1) and 0.1-2.3 mg g(-1), respectively, a percent composition equivalent to 0.5-12.9% and 1.8-17.1% of TOC, respectively. Approximately 93% of refractory organic matter was categorized into humin. Organic matter originated from marine phytoplankton settled on the surface of sediments and changed to humin or a refractory organic matter. This study contributes to a better understanding of the organic matter degradation characteristics of coastal marine sediments and provides important parameters for estimating carbon budget and carbon cycling in coastal sea systems.
Elsevier BV, 2020年09月, Marine Chemistry, 225, 103854 - 103854, 英語[査読有り]
研究論文（学術雑誌）
© 2020, © 2020 Japanese Society of Soil Science and Plant Nutrition. The stability of black soil carbon in the deep layers of Japanese volcanic ash soil (i.e., buried A horizons) is often explained by its unique chemical (molecular structure) and physical (associated with short-range-order minerals) recalcitrance. However, the stability of black soil C in buried A horizons may be changed by labile C supply for soil microbes. Here, we hypothesized that the mineralization of black soil C in buried A horizons of Japanese volcanic ash soil could be easily accelerated by a supply of labile C (i.e., a priming effect; PE). To test our hypothesis, we investigated the direction and magnitude of the PE with a buried A horizon in Japan using 13C-labeled glucose (2.188 atom %) in a short-term (21 days) incubation study. We also investigated the effect of mineral nitrogen (N), which could contribute to microbial activity in this incubation study. We found that a positive PE occurred by glucose supply with (182%) or without (181%) mineral N input over the 21-day incubation, and its values were very similar to the PE ratios previously reported in other deep soils. The estimated mean residence time (MRT) of black soil C considering PE was clearly accelerated by glucose supply, regardless of mineral N input, compared with the initial soil MRT. These results strongly support our hypothesis that the mineralization rate of black soil C in buried A horizons is easily accelerated by a labile C supply, and it also demonstrates important implications for the effects of global warming on buried A horizons (e.g., increased root exudation, fine root biomass supply, and N deposition) in Japanese volcanic ash soils.
2020年, Soil Science and Plant Nutrition[査読有り]
研究論文（学術雑誌）
- Japanese Society of Microbial Ecology, 2020年, Microbes and Environments, 35 (1), n/a - n/a, 英語
[査読有り]
研究論文（学術雑誌）
© 2019, Coastal and Estuarine Research Federation. To clarify the effects of crab burrows on variation in sediment CO2 flux in mangrove forest, we measured the traits of crab burrows (density and entrance area size) and the CO2 flux rate from sediment surfaces, in areas with and without burrows, in a subtropical mangrove forest on Ishigaki Island, southwestern Japan. Burrow density and entrance area showed significant differences among seasons (warm, middle, and cool) and mangrove zones (upper-, middle-, and downstream), which may have depended on crab phenology, life cycle, and species composition. The sediment CO2 flux rate was significantly higher at plots with crab burrows (B+) than at those without burrows (B−) in each zone and season. However, standardized sediment CO2 flux rate by burrow surface area at B+ plots did not differ significantly from that at B− plots. In addition, there were no significant differences in sediment temperature and sediment water content between the two types of plots. Moreover, the level of microbial respiration differed significantly between sediments collected from the deep part and those collected from either the ground surface part or burrow walls. These results suggest that crab burrows increase sediment CO2 flux from the mangrove forest floor by increasing the sediment–atmosphere interface area, thereby inducing a change to aerobic conditions in the sediments around burrows. Therefore, the seasonal and spatial effect of crab burrows on the forest floor should be considered when evaluating sediment CO2 flux and examining the role of the mangrove ecosystem as a carbon sink.
2020年01月, Estuaries and Coasts, 43 (1), 102 - 110, 英語[査読有り]
研究論文（学術雑誌）
© 2019 Elsevier Ltd The molecular composition of soil organic matter (SOM) is important in understanding the elemental cycling of mangrove forests. The aim of the study was to investigate the SOM compositional changes with time in a mangrove mineral soil, which is rarely reported in the literature. A 1-m soil core was collected in the subtropical Fukido River mangrove forest (Okinawa, Japan) and separated into 25-cm sections. Humic acids (HA) and fulvic acids (FA) were extracted from the soil, and their relative abundance and chemical characteristics were analyzed by elemental analysis, stable carbon analysis, spectrophotometric measurement, liquid-state 13C nuclear magnetic resonance spectroscopy, and radiocarbon dating. HA exhibited a clear depth trend in the chemical characteristics, with lower H/C and N/C ratios, higher aromaticity, and higher phenolic 13C NMR peaks in the deeper sections. FA did not show such a clear depth trend, and was enriched in O-containing functional groups. A combination of radiocarbon dating and structural analysis of HA indicated that the rapid structural changes in HA (H/C and N/C) occurred during the first years–decades, followed by a gradual change over a time scale of several hundred (~500) years, and these structural changes were best explained by an increase in the relative phenolic C contents. These results show that mangrove SOM is a complex mixture of organic fractions with different biogeochemical reactivities.
Elsevier {BV}, 2019年12月, Estuarine, Coastal and Shelf Science, 231, 106478[査読有り]
研究論文（学術雑誌）
- 2019年12月, SOIL SCIENCE AND PLANT NUTRITION, 65 (6), 557 - 565, 英語The effects of fertilization treatments and cropping systems on long-term dynamics and spectroscopic characteristics of dissolved organic matter in paddy soil.
[査読有り]
研究論文（学術雑誌）
- 2019年11月, Scientific Reports, 9, 16961, 英語Effect of biochar addition on leaf-litter decomposition at soil surface during three years in a warm-temperate secondary deciduous forest, Japan.
[査読有り]
研究論文（学術雑誌）
- 2019年10月, SOIL SCIENCE AND PLANT NUTRITION, 65 (5), 444 - 450, 英語Priming effect of the addition of maize to a Japanese volcanic ash soil and its temperature sensitivity: a short-term incubation study.
[査読有り]
研究論文（学術雑誌）
- 2019年10月, Polar Data Journal, 3, 37 - 45, 英語
[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2019年07月, Water Research, 163, 114901 - 114901, 英語
[査読有り]
研究論文（学術雑誌）
- Elsevier, 2019年04月, Estuarine, Coastal and Shelf Science, 英語Changes in dissolved organic matter composition and dynamics in a subtropical mangrove river driven by rainfall.
[査読有り]
研究論文（学術雑誌）
The organic-Fe association in Scottish freshwater rivers has received little attention compared with in the estuarine mixing zone. We collected 201 water samples from rivers and lakes in Scotland across different sampling years and seasons. Relationships among the hydrophobic (HPO) fraction of dissolved organic matter (DOM), specific UV absorbance (SUVA(254)), and dissolved metals (Al and Fe) were examined to better understand their co-transportation in Scottish waters. The average DOM, HPO fraction, Fe, and Al concentrations for all the samples co-varied and were lower during winter than during summer. There was a strong positive correlation between DOM and HPO fraction concentrations (R-2=0.99, p<0.0001). A significant positive correlation was also found between the HPO fraction and Fe and Al concentrations. The regression slope indicating the overall relationships between the HPO fraction and Fe concentrations differed by as much as 12 times depending on both the sampling period and the river. These slope differences were not significantly determined by the chemical structures of DOM, SUVA(254), or Al and Cu concentrations. These results suggest that the Fe transport capacities vary among the Scottish rivers because of other factors such as seasonal effects (temperature and the level of water table) and a suspended solid concentration in the water column.
Springer, 2019年03月, Limnology, 20 (2), 215 - 224, 英語[査読有り]
研究論文（学術雑誌）
An anion exchange resin, diethylaminoethyl (DEAE) Sepharose((R)), was utilized for the isolation of dissolved organic matter (DOM) from fresh waters as an alternative to the discontinued DEAE cellulose. We used the following two chemically distinct model DOM samples to determine the optimized adsorption conditions onto DEAE Sepharose: the International Humic Substances Society's standard samples, Suwannee River Fulvic Acid (FA) and Pony Lake FA. The optimized conditions consisted of the following: a contact time of 1h (with shaking), a resin dosage of 1mlmgC(-1), and a dissolved organic carbon (DOC) concentration range of 1-100mgCl(-1). In addition, we examined the distribution of the DOM fractions extracted with DEAE Sepharose and DAX-8 from Lake Biwa (Japan) and Scottish river DOM samples. The majority of DOM (70% and 65%) was extracted by both of the resins. As indicated by H-1 NMR, the evapo-concentrate (bulk DOM), the DEAE Sepharose fraction and the DAX-8 fraction from the Scottish DOM sample had substantial similarity in their proton distributions, while those of a clear-colored, low DOC sample (Lake Biwa) showed different NMR spectra. These findings highlight a need to pay more attention to the extraction selectivity of resins for experimentally challenging' samples.
Springer, 2019年03月, Limnology, 20 (2), 153 - 162, 英語[査読有り]
研究論文（学術雑誌）
- Taylor and Francis, 2019年01月, SOIL SCIENCE AND PLANT NUTRITION, 65 (1), 11 - 19, 英語
[査読有り]
研究論文（学術雑誌）
Uptake of six particle-reactive and/or redox-sensitive radionuclides (210Pb, 234Th, 7Be, 59Fe, 237Np and 233Pa) by 14 humic acids (HAs) was investigated in artificial groundwater under mildly acidic conditions (pH~5.5). In HA-groundwater slurry, Pb, Be, Fe and Pa bound strongly to particulate HA (> 0.45 μm), supporting their application as tracers of soil erosion. Th bound strongly to the colloidal HA (3 kDa-0.45 μm) and as such, would not be a good candidate as a tracer for monitoring soil erosion. HAs likely reduced the oxidized neptunyl form (Np(V)O4 +) to Np(IV) based on its enhanced particle-reactivity and Np uptake by particulate HAs, partially retarding the movement of anthropogenic 237Np in field polluted environments. Particulate/colloidal carbonyl/O-aryl (likely through hydroquinone/quinone) functionalities in the HA correlated to Np and Pa uptake, but only particulate O-aryl functionalities was responsible for Fe uptake. The carboxylate- and carbonyl/O-aryl-containing organic functionalities in the HA correlated strongly with Th uptake. In contrast, no significant correlations between organic parameters and Pb or Be uptake implied their predominance of uniform surface adsorption onto particles. This study provides novel insight into the binding of six radionuclides with different organic functionalities of three size fractions, as well as its possible impact on their application in the soil-tracing research.
Nature Publishing Group, 2018年12月01日, Scientific Reports, 8 (1), 4795, 英語[査読有り]
研究論文（学術雑誌）
- 乾燥汚泥・竹チップ混和堆肥の熱水抽出液の特性評価と堆肥施用がダイズ（丹波黒大豆）の生育に及ぼす影響
<p>乾燥汚泥の農地還元を促進しバイオマス利用を増加させることを目的として，地域の低利用資源である汚泥と竹の破砕物（竹チップ）を混和して堆肥化することを試みた．堆肥の熱水抽出液の特性および圃場への堆肥施用による植物生育への影響について検討した．乾燥汚泥と竹チップの混合割合を5 : 5, 7 : 3, 9 : 1にした3区の堆肥を設けた．熱水抽出液によるコマツナの発芽阻害試験おこない，同液のpH, EC, DOC, DTN, E2/E3, SUVA<sub>254</sub>について特性を調べた．加えて，作製した堆肥（50日目）を用いて地域特産品である「丹波黒大豆」の圃場栽培試験をおこなった．</p><p>作成した堆肥は2週間が経過すると臭気の原因の一部であるアンモニア濃度が低下した．熱水抽出液の特性については，pH, EC, E2/E3は日数経過によって低下傾向にありSUVA<sub>254</sub>は上昇傾向にあることから堆肥化過程における腐熟の進行が示唆された．発芽阻害試験では9 : 1区の発芽阻害が堆肥化初期において顕著であったが，腐熟の進行に従って阻害は軽減された．5 : 5堆肥区では阻害が認められず，重金属において基準値を超えるものはなかったことからも作物生育や安全性に悪影響を及ぼす可能性は極めて低いと考えられる．</p><p>圃場栽培試験では堆肥施用区において丹波黒大豆の主茎長，着莢数が1％水準で有意に増加した．収量に有意な差は認められなかったものの，9 : 1試験区で最大値を示し，少なくとも負の影響は認められなかった．</p><p>これらの結果より，乾燥汚泥と竹チップの混和堆肥は汚泥の臭気問題を軽減することで，地域資源を活用した堆肥として農業生産に利用可能であることが示唆された．</p>
一般社団法人 日本土壌肥料学会, 2018年08月14日, 土肥誌, 89 (4), 295 - 301[査読有り]
Humic substances (HS) are the primary constituents of dissolved organic matter (DOM) and play pivotal roles in aquatic systems. Optical indices of DOM, such as specific UV absorbance (SUVA254), the fluorescence index (FI) and biological index (BIX), have gained wide interest because of their ease of use. In this study, we explored the relationship between HS and the indices in the Trat River Basin (eastern Thailand) from headwaters to the river mouth through the distinct dry and rainy seasons to examine whether changes in index values reflect variability in the relative contribution of HS to DOM, or %HS. The results show that %HS and the indices did not exhibit significant linear relationships (FI and BIX, P > 0.05), or the relationships changed seasonally (SUVA254). However, analyzing the indices versus %HS did show clear DOM composition changes by season with more humic-like or terrestrial material in the rainy season. Relationships between DOM and dissolved iron (dFe) concentrations were also explored. Separating the relationships of DOM versus dFe into HS versus dFe and non-HS versus dFe provides us the opportunity to better understand which fraction contributes more to dFe mobilization. The results indicate stronger positive linear relationships between HS and dFe concentrations independent of river tributary. Overall, this study highlights the importance of quantifying HS for the study of DOM dynamics or compositional changes along a river transect as well as for DOM-induced iron mobilization.
Birkhauser Verlag AG, 2018年07月01日, Aquatic Sciences, 80 (3), 英語[査読有り]
研究論文（学術雑誌）
© 2018 Cambridge University Press. The variation in CO2 flux from the forest floor is important in understanding the role of mangrove forests as a carbon sink. To clarify the effects of soil temperature and tidal conditions on variation in CO2 flux, sediment-atmosphere CO2 fluxes were measured between June 2012 and May 2013. We used the closed chamber method for two plots, with a 0.5 m difference in elevation (B, high elevation; R-B, low elevation), in a mangrove forest in south-western Japan. CO2 fluxes were highest in the warm season and showed a weak positive correlation with soil temperature in both forests. Estimated monthly CO2 flux showed moderate seasonal variation in accordance with the exposure duration of the soil surface under tidal fluctuation. Additionally, measured CO2 flux and soil temperature were slightly higher in the R-B plot than the B plot, although estimated annual CO2 flux was higher in the B plot than the R-B plot due to different exposure durations. These results suggest that variation in the exposure duration of the forest floor, which changes seasonally and microgeographically, is important in evaluating the annual CO2 flux at a local scale and understanding the role of mangrove ecosystems as regulators of atmospheric CO2.
Cambridge University Press, 2018年06月, Journal of Tropical Ecology, 34 (4), 268 - 275, 英語[査読有り]
研究論文（学術雑誌）
The Selenga River Basin (Mongolia and Russia) has suffered from heavy metal contamination by placer gold mining and urban activities in recent decades. The objectives of this study were to provide the first distribution data of dissolved organic matter (DOM) and humic substances (HS) in this data-scarce region, and to investigate their association with dissolved and colloidal metals. Two sampling campaigns were conducted in August of 2013 and 2014. A constant proportion of HS (%HS coefficient of variation of 2%) was observed from the headwater of Tuul River to the end of the delta before Lake Baikal, spanning > 1000 km in distance. The relationships were determined as [HS] = 0.643 × [DOM] (R2 = 0.996, P < 0.001), and this value (%HS = 64.3) is recommended as an input parameter for metal speciation modeling based on samples collected from the rivers. The DOM and metal (Al and Fe) concentrations in samples doubled through the Zaamar Goldfield mining area, but the influence was mitigated by mixing with the larger Orkhon River, which has better water quality. Metals were mainly present as colloids and had a strong positive correlation with DOM (Al r = 0.81, P < 0.01 Fe r = 0.61, P < 0.01), suggesting that DOM sustains colloidal Al and Fe in solution and they are co-transported in the Selenga River Basin. Land use changes affect water quality and metal speciation and therefore have major implications for the fate of metals.
Springer Verlag, 2018年04月01日, Environmental Science and Pollution Research, 25 (12), 11948 - 11957, 英語[査読有り]
研究論文（学術雑誌）
- 2018年04月, Aquatic Sciences, 80 (26), 英語Contribution of humic substances to dissolved organic matter optical properties and iron mobilization.
[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2018年, 日本土壌肥料学会講演要旨集, 64 (0), 21 - 21, 日本語
- 一般社団法人 日本土壌肥料学会, 2018年, 日本土壌肥料学会講演要旨集, 64 (0), 244 - 244, 日本語
[査読有り]
In order to examine the influence of the HA molecular composition on the partitioning of Pu, ten different kinds of humic acids (HAs) of contrasting chemical composition, collected and extracted from different soil types around the world were equilibrated with groundwater at low Pu concentrations (10(-14) M). Under mildly acidic conditions (pH similar to 5.5), 29 +/- 24% of the HAs were released as colloidal organic matter (>3 kDa to <0.45 mu m), yet this HA fraction accounted for a vast majority of the bound Pu, 76 +/- 13% on average. In comparison, the particulate HA fraction bound only 8 +/- 4% on average of the added Pu. The truly dissolved Pu fraction was typically <1%. Pu binding was strongly and positively correlated with the concentrations of organic nitrogen in both particulate (>0.45 mu m) and colloidal phases in terms of activity percentage and partitioning coefficient values (logK(d)). Based on molecular characterization of the HAs by solid state C-13 nuclear magnetic resonance (NMR) and elemental analysis, Pu binding was correlated to the concentration of carboxylate functionalities and nitrogen groups in the particulate and colloidal phases. The much greater tendency of Pu to bind to colloidal HAs than to particulate HA has implications on whether NOM acts as a Pu source or sink during natural or man-induced episodic flooding.
AMER CHEMICAL SOC, 2017年10月, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 51 (20), 11742 - 11751, 英語[査読有り]
研究論文（学術雑誌）
- 2017年08月, Humic Substances Research, 13 (1), 5 - 12, 英語Transformation of soil fulvic acid by Immobilized laccase from Trametes villosa.
[査読有り]
研究論文（学術雑誌）
Although mangrove forests are one of the most well-known soil organic carbon (SOC) sinks, the mechanism underlying SOC accumulation is relatively unknown. High net primary production (NPP) along with the typical bottom-heavy biomass allocation and low soil respiration (SR) have been considered to be responsible for SOC accumulation. However, an emerging paradigm postulates that SR is severely underestimated because of the leakage of dissolved inorganic carbon (DIC) in groundwater. Here we propose a simple yet unique mechanism for SOC accumulation in mangrove soils. We conducted sequential extraction of water extractable organic matter (WEOM) from mangrove soils using ultrapure water and artificial seawater, respectively. A sharp increase in humic substances (HS) concentration was observed only in the case of ultrapure water, along with a decline in salinity. Extracted WEOM was colloidal, and <= 70% of it re-precipitated by the addition of artificial seawater. These results strongly suggest that HS is selectively flocculated and maintained in the mangrove soils because of high salinity. Because sea salts are a characteristic of any mangrove forest, high salinity may be one of mechanisms underlying SOC accumulation in mangrove soils. (C), 2017 Published by Elsevier Ltd.
PERGAMON-ELSEVIER SCIENCE LTD, 2017年06月, CHEMOSPHERE, 177, 51 - 55, 英語[査読有り]
研究論文（学術雑誌）
The Selenga River contributes to 50% of the total inflow to Lake Baikal. Large tracts of the Selenga River Basin have been developed for industry, urbanization, mining, and agriculture, resulting in the release of suspended solids (SS) that affect downstream water quality and primary productivity. This study addressed SS as the main factor controlling pollutant transport and the primary indicator of land degradation in the Selenga River system. Tributaries with larger areas dedicated to agricultural use had higher SS concentrations, reaching 862 mg L-1, especially during the high runoff and intensive cultivation season. Although the large SS flux was detected in the main river, the small tributaries were distinguished by high SS concentrations. The high SS concentration corresponded to widespread development in the watershed. Watersheds with high potential of SS release are sensitive to intensive land uses. SS in the river system had a constant elemental composition consisting mainly of Fe and Al oxides, indicating that surface soils were major constituents of the tributary SS. Three heavymetals (Zn, Cu, and Cr) appeared in high concentrations downstream of urban and mining areas (two-to sixfold increases), indicating that these contaminants are carried by SS. At two tributary junctions, the concentration of contaminants on the SS decreased due to a large influx of SS with low heavy metal contents. Changes in electric conductivity and pH at downstream of tributary junctions enhanced the sedimentation of SS and the removal of contaminants from the water phase after aggregation of the SS. Land use changes in the tributary watersheds are major controlling factors for the fate of contaminants in the river system.
SPRINGER, 2017年05月, ENVIRONMENTAL EARTH SCIENCES, 76 (9), 346 - 361, 英語[査読有り]
研究論文（学術雑誌）
Dissolved organic matter (DOM) strongly affects water quality within boreal forest ecosystems. However, how the quality of DOM itself changes spatially is not well understood. In this study, to examine how the diversity of DOM molecules varies in water moving through a boreal forest, the number of DOM molecules in different water samples, i.e., rainwater, throughfall, soil water, groundwater, and stream water was determined using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in Norway spruce and Scots pine stands in eastern Finland during May and June 2010. The number of molecular compounds identified by FT-ICR MS (molecular diversity) ranged from 865 to 2,194, revealing large DOM molecular diversity in the water samples. Additionally, some of the molecular compounds were shared between different water samples. The DOM molecular diversity linearly correlated with the number of low-biodegradable molecules, such as, lignin-like molecules (lignins), but not with dissolved organic carbon concentration. The number of lignins shared between different sampling locations was larger than that of any other biomolecular class. Our results suggest that low-biodegradable molecules, especially lignins, regulate spatial variations in DOM molecular diversity in boreal forests.
NATURE PUBLISHING GROUP, 2017年02月, SCIENTIFIC REPORTS, 7, 42102, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2017年, 日本土壌肥料学会講演要旨集, 63 (0), 89 - 89, 日本語
[査読有り]
Mangrove forests are tropical forests with carbon-rich soil. Dissolved organic matter (DOM) is important for investigating the mangrove carbon cycle and ecological roles because the forests connect the river tributaries with the coast via rivers running through them. The ecological functions and turnover rate of DOM depend on its composition. Therefore, we characterized the DOM of the Fukido River in a mangrove forest by analyzing the percentage of humic substances (%HS) in DOM. Water sampling from the headwaters to the sea revealed that %HS declined going toward the sea due to mixing with low-%HS seawater and the possible selective precipitation of HS into mangrove soil. The headwaters had a higher %HS than most clear water rivers and may be an important supply of HS for mangrove and coastal areas. The sequential extraction of water-extractable organic matter from mangrove soil (sampling depth: 0–25 cm) using ultrapure water indicated a significant increase in HS concentration along with a decline in salinity. These results suggest that high salinity is responsible for the selective accumulation of HS in mangrove soil, which can be a key to elucidating the mechanism of organic matter accumulation in mangrove soils.
Tohoku University, 2017年, 日本生態学会誌, 67 (2), 85 - 93, 日本語[査読有り]
研究論文（学術雑誌）
nonionic macroporous resin, Amberlite (R) XAD-8, or its substitute, Supelite (TM) DAX-8, is used when isolating or quantifying aquatic humic substances (AHS). However, the effect of salinity on the adsorption behavior of AHS onto the resin is yet to be confirmed, rendering the possibility of salinity-induced changes in the values of quantified amounts or characteristics of AHS obtained from a salty system. To verify the results of quantification and isolation of AHS using the resin in different salinity systems, the effect of salinity on such quantitative analyses of AHS has been examined. It has been concluded that the salinity effect is in general trivial and will not hinder comparison of results regardless of sample solution salinity. (C) 2015 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2016年03月, CHEMOSPHERE, 146, 129 - 132, 英語[査読有り]
研究論文（学術雑誌）
In order to assess how environmental factors are affecting the distribution and migration of radioiodine and plutonium that were emitted from the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident, we quantified iodine and Pu-239,Pu-240 concentration changes in soil samples with different land uses (urban, paddy, deciduous forest and coniferous forest), as well as iodine speciation in surface water and rainwater. Sampling locations were 53-63 km northwest of the FDNPP within a 75-km radius, in close proximity of each other. A ranking of the land uses by their surface soil (<4 cm) stable I-127 concentrations was coniferous forest > deciduous forest > urban > paddy, and Pu-239,Pu-240 concentrations ranked as deciduous forest > coniferous forest > paddy > urban. Both were quite distinct from that of Cs-134 and Cs-137: urban > coniferous forest > deciduous forest > paddy, indicating differences in their sources, deposition phases, and biogeochemical behavior in these soil systems. Although stable I-127 might not have fully equilibrated with Fukushima-derived I-129, it likely still works as a proxy for the long-term fate of I-129. Surficial soil I-127 content was well correlated to soil organic matter (SOM) content, regardless of land use type, suggesting that SOM might be an important factor affecting iodine biogeochemistry. Other soil chemical properties, such as Eh and pH, had strong correlations to soil I-127 content, but only within a given land use (e.g., within urban soils). Organic carbon (OC) concentrations and Eh were positively, and pH was negatively correlated to I-127 concentrations in surface water and rain samples. It is also noticeable that I-127 in the wet deposition was concentrated in both the deciduous and coniferous forest throughfall and stemfall water, respectively, comparing to the bulk rainwater. Further, both forest throughfall and stemflow water consisted exclusively of organo-iodine, suggesting all inorganic iodine in the original bulk deposition (similar to 28.6% of total iodine) have been completely converted to organo-iodine. Fukushima-derived Pu-239,Pu-240 was detectable at a distance similar to 61 km away, NW of FDNPP. However, it is confined to the litter layer, even three years after the FDNPP accident-derived emissions. Plutonium-239,240 activities were significantly correlated with soil OC and nitrogen contents, indicating Pu may be associated with nitrogen-containing SOM, similar to what has been observed at other locations in the United States. Together, these finding suggest that natural organic matter (NOM) plays a key role in affecting the fate and transport of I and Pu and may warrant greater consideration for predicting long-term stewardship of contaminated areas and evaluating various remediation options in Japan. (C) 2016 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2016年03月, JOURNAL OF ENVIRONMENTAL RADIOACTIVITY, 153, 156 - 166, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2016年, 日本土壌肥料学会講演要旨集, 62, 79 - 79, 日本語
- 一般社団法人 日本土壌肥料学会, 2016年, 日本土壌肥料学会講演要旨集, 62, 113 - 113, 日本語
- 一般社団法人 日本土壌肥料学会, 2016年, 日本土壌肥料学会講演要旨集, 62, 21 - 21, 日本語
Aquatic humic substances (AHSs) are major constituents of dissolved organic matter (DOM) in freshwater bodies. We performed quantitative analyses of AHSs in Japanese lake and wetland waters, focusing mainly on clear waters with low carbon contents, by using a resin isolation technique based on the carbon concentration in the AHSs of each sample. The proportion of AHS to DOM in the clear waters ranged from 38.4 to 64.1 %; these proportions are lower than those widely assumed for freshwater of 20-80 %. Moreover, the proportions of AHSs in DOM were not constant, so regression analysis cannot be used to predict the AHS concentration from the DOM concentration. Thus, AHS and DOM concentrations must be determined separately for each water sample.
SPRINGER JAPAN KK, 2016年01月, LIMNOLOGY, 17 (1), 1 - 6, 英語[査読有り]
研究論文（学術雑誌）
- 日本水処理生物学会, 2015年12月, 日本水処理生物学会誌, 51 (4), 105 - 114, 日本語琵琶湖水中のフルボ酸のOECD試験法による水生生物への急性毒性評価
[査読有り]
研究論文（学術雑誌）
- 2015年04月, Organic Geochemistry, 2015, 153 - 157, 英語Quantitative monitoring of aquatic humic substances in Lake Biwa, Japan, using the DAX-8 batch method based on carbon concentrations.
[査読有り]
研究論文（学術雑誌）
- 2015年02月, Catena, 127, 241 - 221, 英語Relationship between stable carbon and nitrogen isotope ratios of humic acids extracted from Andisols and non-Andisols.
[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2015年, 日本土壌肥料学会講演要旨集, 61, 14 - 14, 日本語
Humic substances (HSs) are ubiquitous in various aquatic systems and play important roles in many geochemical processes. There is increasing evidence of the presence of HSs in deep groundwater; nevertheless, their ion binding properties are largely unknown. In this study we investigated the physicochemical and ion-binding properties of humic and fulvic acids extracted from deep sedimentary groundwater. The binding isotherms of protons (H+) and copper (Cu2+) were measured by potentiometry and fitted to the NICA-Donnan model, and the obtained parameters were compared with the generic parameters of the model, which are the average parameters for HSs from surface environments. The deep groundwater HSs were different from surface HSs, having high aliphaticities, high sulfur contents, and small molecular sizes. Their amounts of acidic functional groups were comparable to or slightly larger than those of surface HSs; however, the magnitude of Cu2+ binding to the deep groundwater HSs was smaller. The NICA-Donnan model attributed this to the binding of Cu2+ to chemically homogeneous low affinity sites, which presumably consist of carboxylic groups, via mono-dentate coordination at relatively low pH. The binding mode tended to shift to multi-dentate coordination with carboxylic groups and more heterogeneous alcoholic/phenolic groups at higher pH. X-ray absorption spectroscopy also revealed that Cu2+ binds to O/N containing functional groups and to a lesser extent S containing functional groups as its divalent from. This study shows the particularity of the deep groundwater HSs in terms of their physicochemical and ion-binding properties, compared with surface HSs.
ROYAL SOC CHEMISTRY, 2015年, ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS, 17 (8), 1386 - 1395, 英語[査読有り]
研究論文（学術雑誌）
- 2014年10月, 滋賀県琵琶湖環境科学研究センター研究報告書, (7), 1 - 24, 日本語琵琶湖における新たな水質管理指標に関する研究
研究論文（大学，研究機関等紀要）
- Committee of The 17th meeting of the International Humid Substances Society, 2014年09月, The 17th meeting of the International Humid Substances Society, 214 - 215, 英語The effect of Humic acid on the Acute Toxicity of Benzo[a]pyrene to Algae, Pseudokirchneriella subcapitata.
[査読有り]
研究論文（国際会議プロシーディングス）
- Committee of The 17th meeting of the International Humid Substances Society, 2014年09月, The 17th meeting of the International Humid Substances Society, 254 - 255, 英語Comparison of Structural Properties of Humic-Like Substances in Atmospheric Fine Aerosol Isolated by Different Methods.
[査読有り]
研究論文（国際会議プロシーディングス）
- Japanese Humic Substances Society, 2013年10月, Humic Substances Research, 10 (1), 1 - 9, 英語Investigations of environmental changes based on chemical analyses of humic substances
[査読有り][招待有り]
The dynamics of the polyaromatic structures of black humic acids (HAs), which are presumably derived from charred materials, are of significant interest for the global carbon cycle. However, the details of those dynamics are not yet well understood. We investigated differences in the degree of darkness (A(600)/ C values), isotopic ratios (delta C-13, delta N-15, and Delta C-14 values), and C-13 NMR spectra of size-separated black HAs extracted from Japanese volcanic ash soils in order to estimate the variations in the polyaromatic structures of black HAs during ca. 100 years of natural reforestation of Japanese pampas grassland. For several hundred years, all the study sites were managed similarly as grassland by burning. Subsequently, their management differed: at site G (Miscanthus sinensis: C4 plant), maintenance as of the time of this study was still performed by mowing, while at sites P (Pinus densiflora: C3 plant) and Q(Quercus crispula: C3 plant), maintenance was discontinued ca. 30 and 100 years ago, respectively. Thus, the sites range from grassland (site G) to coniferous forest (site P) to broad-leaved forest (site Q). For all HA size fractions at all sites, we found that delta C-13 values correlate positively with delta N-15 values, although the gradients are much lower for fractions of small to medium molecular size than for fractions of medium to large molecular size (denoted as lower-size and higher-size fractions, respectively). Overall, for the lower-size fractions, the contribution ratio of C4-plant-derived carbon shows a significant positive correlation with A(600)/C values and a negative correlation with Delta C-14 values, and their aromatic characteristics are greater than those of higher-size fractions within the same black HA. Furthermore, the relative proportion of lower-size fractions decreases with reforestation, especially from site P to Q The delta C-13 values for all size fractions are similar for sites G and P. but are relatively low for site Q. The aryl C contents of the lower-size fractions are lower and the O-alkyl C contents and the aliphaticity (alkyl C:O-alkyl ratio) are clearly higher for sites P and Q than for site G. These results strongly suggest that stimulation of HA biodegradation might be achievable by continuous input of new plant litter during reforestation, even for lower-size HA polyaromatic structures, despite the fact that lower-size HAs biodegrade more slowly than higher-size HAs. (C) 2012 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2013年02月, SOIL BIOLOGY & BIOCHEMISTRY, 57, 60 - 67, 英語[査読有り]
研究論文（学術雑誌）
Characteristics of dissolved humic and fulvic acids in river waters were studied during 2003-2005 at 4 sites located in the headwaters and in the upper and lower Tokachi River, including a lowland tributary site. Fulvic acids from the headwaters to downstream areas have similar elemental composition and C-13-NMR spectra. Humic acids have similar characteristics in the Tokachi River system. In contrast, delta C-13 and Delta C-14 values exhibit a decreasing trend from the upper to the lower and tributary sites, although the headwater site has heavier delta C-13 and lower Delta C-14 values than the upper site. Fulvic acids had similar delta C-13 values from the upper to lower sites, but 123 parts per thousand higher in Delta C-14 than those of humic acids on average. The delta C-13 and Delta C-14 values exhibited differences in downward variation for humic and fulvic acids. In the Tokachi River system, these results suggest that differences in transport pathways and residence times of humic and fulvic acids reflect differences in the delta C-13 and Delta C-14 values in a single river basin.
UNIV ARIZONA DEPT GEOSCIENCES, 2013年, RADIOCARBON, 55 (2-3), 1007 - 1016, 英語[査読有り]
研究論文（学術雑誌）
We analyzed the physicochemical and spectroscopic properties of three charred plant fragments (CPF) that were isolated from three volcanic ash soils in Japan. Values of delta C-13 showed that the CPF originated from C-3 and C-4 plants. The contribution ratio of C-4 plants to the CPF was much higher in Soils 1 and 3 than in Soil 2. Values of delta N-15 of the CPF were higher in Soil 3 isolated from the deeper soil horizons. Light reflectance values suggested that part of the CPF experienced combustion temperatures higher than 400 degrees C in Soil 2 and less than 400 degrees C in the other soils, respectively. Atomic [H]/[C] and [O]/[C] ratios suggested that the CPF were subjected to weathering ( oxidative degradation and hydrolysis) in soil for a long period after burning. The degree of weathering was considered to be larger in Soils 1 and 3. Spectra of C-13-NMR of the CPF, except the area of alkyl-C, were similar. Infrared (IR) spectra of three CPF, except aliphatic C-H stretching, were also similar to each other. The X-ray diffraction patterns of the CPF in Soil 2 clearly revealed the presence of graphite-like structure. From these findings, it was assumed that the physicochemical and spectroscopic characteristics of the CPF were strongly influenced by the type of burnt vegetation.
LIPPINCOTT WILLIAMS & WILKINS, 2012年12月, SOIL SCIENCE, 177 (12), 695 - 700, 英語[査読有り]
研究論文（学術雑誌）
- 日本ペドロジー学会, 2012年12月, Pedologist, 56 (2), 47 - 57, 英語Features and properties of Chernozemic soils and humic substances in eastern Ukraine
[査読有り]
研究論文（学術雑誌）
- A Method for Quantitative Analysis of Aquatic Humic Substances in Clear Water Based on Carbon Concentration
Aquatic humic substances (AHSs) are major constituents of dissolved organic matter (DOM) in freshwater, where they perform a number of important ecological and geochemical functions, yet no method exists for quantifying all AHSs. We have developed a method for the quantitative analysis of AHSs based on their carbon concentration. Our approach includes: (1) the development of techniques for clear-water samples with low AHS concentrations, which normally complicate quantification; (2) avoiding carbon contamination in the laboratory; and (3) optimizing the AHS adsorption conditions.
JAPAN SOC ANALYTICAL CHEMISTRY, 2012年10月, ANALYTICAL SCIENCES, 28 (10), 1017 - 1020, 英語[査読有り]
研究論文（学術雑誌）
The binding of europium to dissolved humic substances (HSs), isolated from deep groundwater in the Horonobe area, Hokkaido, Japan, was evaluated by means of a three-dimensional fluorescence quenching technique. The Ryan and Weber model, extended by the charge neutralization model, was applied to the fluorescence quenching profiles to evaluate the conditional binding constants (K) at pH5.0 and an ionic strength of 0.1. The K values for fulvic (FA) and humic acids (HA) in the Horonobe groundwater were comparable with each other regardless of the position of the fluorescence peaks. As compared with HSs from Lake Biwa, Aldrich, and Dando soil, the K values of the Horonobe groundwater HSs were the smallest, indicating that the Horonobe groundwater HSs have a lower affinity for the binding of europium compared to the HSs from surface environments. In addition, the results of the Aldrich-based comparison indicate that the Horonobe groundwater HSs have a lower binding affinity for trivalent lanthanides/actinides compared with other groundwater HSs obtained from different aquifer. These findings demonstrate that the characteristics and origin of deep groundwater HSs needs to be taken into consideration assessing the effects of HSs on the speciation of radionuclides in deep groundwater in geological disposal systems.
TAYLOR & FRANCIS LTD, 2012年08月, JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY, 49 (8), 804 - 815, 英語[査読有り]
研究論文（学術雑誌）
Seasonal characteristics of surface water fulvic acids (FAs) isolated from Japanese clear-water lakes were investigated. Qualitative changes in Lake Biwa and Lake Tankai FAs were determined and compared. Although the relative molecular weights determined by high-performance size-exclusion chromatography did not change remarkably, the elemental compositions, E-600(1%) values and H-1- and C-13-NMR spectral properties varied with the season. Both the H/C and N/C ratios for Lake Biwa FAs tended to be higher than those for Lake Tankai FAs, but O/C ratios were lower and decreased from spring to winter. The E-600(1%) values suggested that Lake Biwa FAs contained lower levels of unsaturated structures than Lake Tankai FAs, and the amounts of these structures increased in winter. The H-1 and C-13 NMR spectra indicated that Lake Biwa FAs are richer in saturated aliphatic chains, especially in spring. Overall, Lake Biwa FAs exhibited clearer seasonal changes in these characteristics than Lake Tankai FAs, suggesting that the seasonal variation may depend on changes in aquatic microbial activities.
SPRINGER TOKYO, 2012年04月, LIMNOLOGY, 13 (1), 45 - 53, 英語[査読有り]
研究論文（学術雑誌）
- 13C NMRならびに高速サイズ排除クロマトグラフィー（HPSEC）による土壌腐植酸の特徴付け
Humic acids (HAs), the major components of organic matter in soils, play an important role in global carbon cycling and regulation of the mobility and fate of plant nutrients and organic and inorganic pollutants. Despite intensive research on soil HAs during the past 200 years, the chemical nature of HAs is not fully understood. Studies using a variety of spectroscopic techniques have led to major advances in understanding the chemical nature of HAs, but the macromolecule structure and chemical heterogeneity of HAs is a serious hindrance. Therefore, the characterization and grouping of HAs with various chemical natures has been studied. C-13 NMR analysis and high performance size exclusion chromatography (HPSEC) are the most powerful and popular tools in this study field. We here describe : (i) optimization of the HA measuring condition in both analyses, (ii) characterization of the chemical nature among HAs in various soil origins, and (iii) HA grouping by statistical analysis based on information data obtained by C-13 NMR and HPSEC analyses. Thirty-three of HAs from various soil origins were analyzed and, conclusively, they were classified clearly by their chemical nature.
JAPAN SOC ANALYTICAL CHEMISTRY, 2012年04月, 分析化学, 61 (4), 287 - 298, 日本語[査読有り][招待有り]
研究論文（学術雑誌）
- Characterization of Humic Like Substances in atmospheric aerosols using TMAH thermochemolysis GC/MS
Humic like substances (HULISs) extracted from atmospheric aerosols in Osaka city were analyzed by thermochemolysis with tetramethylammonium hydroxide (TMAH) pyrolysis GC/MS. Main components assigned on GC/MS chromatograms were fatty acid methyl esters, dicarboxylic acid dimethyl esters, and phenol derivatives. Compositions of the component of the HULISs were different from that of humic substances in soil and HULIS in standard urban aerosols (SRM 1648a), suggesting differences in origin and formation mechanisms from these substances.
JAPAN ASSOC AEROSOL SCI & TECHNOLOGY, 2012年, 29TH SYMPOSIUM ON AEROSOL SCIENCE AND TECHNOLOGY, 2012, 159 - 160, 中国語[査読有り]
研究論文（国際会議プロシーディングス）
We investigated the optical absorption properties (A(400)/C and A(600)/C values), elemental composition, weight-averaged molecular weight (Mw), and liquid-state carbon-13 (C-13) nuclear magnetic resonance (NMR) spectroscopy of DAX-8 adsorbed fulvic acid fractions (DAX-8 FAs) in various soils (n = 36) to establish the general trends in their chemical characteristics. We also compared our results with those of humic acids (HAs) obtained from the same soils used in this study and other published data to discuss the differences between them. Our results clearly indicate that DAX-8 FAs with high carboxyl C content have small Mw and low hydrogen to carbon (H/C) ratio. In addition, DAX-8 FAs with high carbonyl C and alkyl C content have high A(400)/C values and H/C ratios, respectively. These results strongly suggest that DAX-8 FAs with high aliphatic chemical properties have low carboxyl C content, large Mw and high H/C ratio. There are significant differences in chemical characteristics among the various soil types in almost all data; e. g., DAX-8 FAs from Podzols showed significantly higher A(400)/C values, larger Mw, higher carbonyl C and O-aryl C content than those obtained from Andosols. However, these two C functional groups are relatively low values in the total C content among all soil types, suggesting that DAX-8 FAs may exhibit only small differences in chemical properties among the various soil types. The comparison with published data regarding soil HAs shows that DAX-8 FAs are characterized by smaller Mw; lower C, H, and nitrogen (N); higher oxygen (O) content; higher O/C; lower O/H ratios; higher carboxyl C content; and lower aryl C content in total C.
TAYLOR & FRANCIS LTD, 2012年, SOIL SCIENCE AND PLANT NUTRITION, 58 (4), 404 - 415, 英語[査読有り]
研究論文（学術雑誌）
- 2011年10月, Humic Substances Research, 8 (1), 13 - 18, 英語Particle sizes of standard humic substances calculated as radii of gyration, maximum diameter and hydrodynamic radii
[査読有り]
研究論文（学術雑誌）
- 2011年10月, Humic Substances Research, 8 (1), 7 - 11, 英語Decolorization of soil fulvic acids by laccases from Trametes versicolor and Trametes villosa
[査読有り]
研究論文（学術雑誌）
High performance size-exclusion chromatography (HPSEC) is useful for the molecular size separation of soil humic acids (HAs), but there is no method available for various HAs with different chemical properties. In this paper the authors propose a new preparative HPSEC method for various soil HAs. Three soil HAs with different chemical properties were fractionated by a Shodex OHpak SB-2004 HQ column with 10 mM sodium phosphate buffer (pH 7.0)/acetonitrile (3:1, v/v) as an eluent. The HAs eluted within a reasonable column range time (12-25 min) without peak tailing. Preparative HPSEC chromatograms of these HAs indicated that non-size-exclusion effects were suppressed. The separated fractions were analyzed by HPSEC to determine their apparent molecular weights. These decreased sequentially from fraction 1 to fraction 10, suggesting that the HAs had been separated by their molecular size. The size-separated fractions of the soil HA were mixed to compare them with unfractionated HA. The analytical HPSEC chromatogram of the mixed HA was almost identical to that of the unfractionated HA. It appears that the HAs do not adsorb specifically to the column during preparative HPSEC. Our preparative HPSEC method allows for rapid and reproducible separation of various soil HAs by molecular size. (C) 2011 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2011年09月, JOURNAL OF CHROMATOGRAPHY A, 1218 (37), 6448 - 6453, 英語[査読有り]
研究論文（学術雑誌）
A five-step sequential extraction procedure was developed for the partitioning of soil aluminum (Al) into five fractions: exchangeable, weakly organic bound, strongly organic bound, inorganic non-crystalline and crystalline. The results obtained by the sequential extraction method for Al were compared with those estimated from single extractions using identical operating conditions applied in each individual sequential fraction. The Al content in the extracts was measured by inductively coupled plasma optical emission spectrometry. The results indicated that the first four steps [potassium chloride (KCl), copper chloride (CuCl(2)), sodium pyrophosphate (Na(4)P(2)O(7)), acid ammonium oxalate ((NH(4))(2)C(2)O(4))] in the sequential procedure could be as effective as single extraction methods at estimating exchangeable Al, weakly organic bound Al, strongly organic bound Al, and non-crystalline Al. However, the crystalline Al content by sequential procedure was not in agreement with single extraction procedures. Further, the sequential extractions resulted in more consistent estimates of the aluminum/silicon (Al/Si) molar ratio and allophane and crystalline Al contents than single extractions method. Results of X-ray diffraction on the soil samples confirm the presence of appreciable amounts of at least three types of crystalline minerals, including gibbsite, vermiculite or halloysite. Our result implies that the sequential method can be more reliable in estimating the various Al forms in Andisols.
WILEY-BLACKWELL, 2011年08月, SOIL SCIENCE AND PLANT NUTRITION, 57 (4), 500 - 507, 英語[査読有り]
研究論文（学術雑誌）
This study reports carbon isotopic ratios (Delta(14)C and delta(13)C) of dissolved humic and fulvic acids in the Tokachi River system, northern Japan. These acids have a refractory feature and they represent the largest fraction of dissolved organic matter in aquatic environments. The acids were isolated using the XAD extraction method from river water samples collected at three sites (on the upper and lower Tokachi River, and from one of its tributaries) in June 2004 and 2005. delta(13)C values were -27.8 to -26.9 parts per thousand for humic and fulvic acids. On the other hand, the Delta(14)C values ranged from -247 to 126 parts per thousand and the average values were -170 +/- 79 parts per thousand for humic acid and -44 +/- 73 parts per thousand for fulvic acid. The difference was attributed to the residence time of fulvic acid in the watershed being shorter than that of humic acid. The large variation suggested that humic substances have a different pathway in each watershed environment.
OXFORD UNIV PRESS, 2011年07月, RADIATION PROTECTION DOSIMETRY, 146 (1-3), 322 - 325, 英語[査読有り]
研究論文（学術雑誌）
- 大規模堆肥化処理方式が牛ふん尿堆肥の腐熟度と腐植化に及ぼす影響
大規模堆肥化処理方式の違いが牛ふん尿堆肥の腐植化に及ぼす影響を調べるために、堆積方式と撹拌方式により製造された堆肥から抽出された腐植物質の化学的および光学的特性を比較した。いずれの方式についても、堆肥化に伴うC／N比や易分解有機物量の減少、幼植物発芽試験における腐熟化の進行が認められた。一方、処理方式により、堆肥から抽出された腐植酸の光学的特性が著しく異なり、堆積方式では腐植酸の黒色化とカルボキシル基などの官能基構造の発達を伴う腐植化の進行が顕著であったのに対し、撹拌方式では堆肥化後も未熟でリグニン構造が残存した腐植酸であった。植物の生育に影響を及ぼす可能性がある動的な腐食物質を水抽出して比較したところ、堆積方式ではフルボ酸の増加と腐植化に伴う構造変化が認められたが、撹拌方式ではフルボ酸が減少した。処理方式が異なる堆肥の圃場還元においては、腐植化の違いを考慮した使い分けが必要である。
農業施設学会, 2011年06月25日, 農業施設, 42 (1), 8 - 17, 日本語[査読有り]
研究論文（学術雑誌）
Japanese volcanic ash soils have very thick and dark-colored A horizons with large amounts of black humic acids which are characterized by their extremely high aromatic structure and stabilities such as black carbon. Nevertheless, the disappearance of the melanic epipedon with a decreasing aromatic C and increasing alkyl C proportion of humic acids was observed in ecological succession for only over 20-30 years. To obtain further details regarding the chemical characteristics of this extraordinary changing process quantitatively, humus quantitative analysis, high-performance size exclusion chromatography (HPSEC), and liquid-state (13)C nuclear magnetic resonance ((13)C NMR) analysis were performed, including those for fulvic acids at the surface of mineral soils (0-20 cm), in the grassland/forest ecotone of volcanic ash soil in Japan. In addition, to obtain information regarding the stability and origin of C which gives the key of changing mechanism of humus along with ecological succession, resistance to hydrogen peroxide oxidation (percent decolorization) and the stable isotope ratio of carbon (delta(13)C) were measured, respectively. The study sites were managed as grassland for several hundred years. Grassland at site 1 was still being maintained by mowing, while the maintenance of sites 2 and 3 was discontinued approximately 30 and more than 100 years ago, respectively. The dominant vegetative cover at site 1 was Miscanthus sinensis, that at site 2 was Pinus densiflora with an understory of Sasa spp., and the vegetation comprised mainly Quercus crispula with an understory of Sasa spp. at site 3.The concentration of humic acids decreased significantly from 60.4 to 47.4 C g kg(-1) in site 2, and then decreased continuously from 47.4 to 30.2 C g kg(-1) in site 3. These trends paralleled those of the total C and N content in soils, and C:N ratio increased along with succession from 11.1 to 17.0 to 19.2, respectively, supporting the hypothesis that changing vegetation from grassland to forest may promote N losses by plant uptake. Simultaneously, the concentration of fulvic acids and humin decreased only slightly throughout the series relative to humic acids. Physicochemical properties appeared to be affected by the succession especially in humic acids. In particular, the apparent content of aryl C moieties in the humic acids of site 2 decreased significantly from 30.0 to 14.9 C g kg(-1), a level similar to site 3. Percent decolorization of humic acids corresponded to their aromaticity: site 1 had a lower value (55.3%) than those of sites 2 and 3 (65.8% and 66.4%, respectively). The delta(13)C values of humic acids ranged from -19.6 to -22.6 parts per thousand: however, the values for sites 1 and 2 were exactly the same. The findings of this study point to the fact that humic acids, but not other humus fractions, significantly changed with decreasing aryl C moieties and stability over the first 30 years of conversion from managed grassland to forest Furthermore, the disappearance of the melanic epipedon in the surface volcanic ash soil evidently contributed to the aryl C, but not alkyl C. content of humic acids, and there might be a difference in the major mechanism of chemical change of humic acids before 30 years and more than 100 years. At least for the first 30 years, it is strongly suggested that C losses and changes in chemical properties of humic adds could be due to extraordinary microbial degradation processes through accelerated plant N uptake along with ecological sccession from grassland to forest in earlier stages. (C) 2010 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2010年10月, GEODERMA, 159 (1-2), 122 - 130, 英語[査読有り]
研究論文（学術雑誌）
- 2010年08月, Proceedings of 19th World Congress of Soil Science, Brisbane, Australia, 英語Effects of ecological succession on chemical characteristics of humic and fulvic acids in a Japanese volcanic ash soil
研究論文（国際会議プロシーディングス）
- 2010年07月, Proceedings of the 15th meeting of International Humic Substances Society, Puerto de la Curz, Spain, 3, 182 - 185, 英語Sorption of pharmaceuticals to humic substances
[査読有り]
研究論文（国際会議プロシーディングス）
- 2010年07月, Proceedings of the 15th meeting of International Humic Substances Society, Puerto de la Curz, Spain, 2, 62 - 65, 英語Similarities of 13C NMR spectroscopic properties of humic acids between black soils–andosols and chernozems
[査読有り]
研究論文（国際会議プロシーディングス）
- 2010年07月, Proceedings of the 15th meeting of International Humic Substances Society, Puerto de la Curz, Spain, 2, 321 - 323, 英語Effect of clay minerals on the fungal degradation of humic acid
[査読有り]
研究論文（国際会議プロシーディングス）
Characteristics of aquatic fulvic acids (FAs) from 10 clear waters in Japan (around the temperate zone) were revealed by several analytical techniques high performance size exclusion chromatography (HPSEC), elemental analysis, liquid-state (13)C NMR spectroscopy, isotopic analyses (delta(13)C and delta(15)N), and compared with those of International Humic Substances Society (IHSS) standard samples including FAs from brown waters (Suwannee, Pony, and Nordic FAs). Generally clear-water FAs were different from brown-water FAs in chemical properties. Weight-average molecular weights (Mw) of the clear-water FAs were similar to each other, whereas their elemental compositions and carbon species distribution were different. The clear-water FAs all exhibited a high proportion of alkyl carbons, which may be attributed to microbial activity. delta(13)C and delta(15)N values of the FAs indicated that there would be a huge gap between origin and chemical structure of clear-water FA. Results of the chemical structural analyses described above were not always linked to those of the isotopic analyses (delta(13)C and delta(15)N). Multivariate statistical analysis, i.e. cluster and principal component analysis was applied to reveal differences or similarities in a more objective manner. The FAs were always classified into two clear-water groups and one brown-water group. Aryl-C and O-Alkyl-C contents were important for the grouping. We speculate that the grouping might depend on the differences of aquatic microbial activity caused by the differences of residence time of water. (C) 2010 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2010年07月, WATER RESEARCH, 44 (13), 3837 - 3846, 英語[査読有り]
研究論文（学術雑誌）
- 2009年12月, Pedologist, 53 (2), 86 - 93, 英語Relationship between distribution of charred plant residues and humus composition in chernozemic soils
[査読有り]
研究論文（学術雑誌）
- 2009年10月, Humic Substances Research, 5/6, 9 - 17, 英語Effects of groundwater humic substances on sorption of Np (V) on sandy materials
[査読有り]
研究論文（学術雑誌）
- 2009年10月, Humic Substances Research, 5/6, 45 - 53, 英語Chemical properties of aquatic fulvic acids isolated from Lake Biwa, a clear water system in Japan
[査読有り]
研究論文（学術雑誌）
- 社団法人日本土壌肥料学会, 2009年09月, 日本土壌肥料学会講演要旨集, 0 (55), 19, 日本語2-2 二次遷移の進行に伴い黒ボク土腐植物質の諸特性はどうなるのか? : 腐植酸とフルボ酸の化学構造特性解析(2.土壌有機・無機化学,2009年度京都大会)
研究論文（学術雑誌）
- 社団法人日本土壌肥料学会, 2009年09月, 日本土壌肥料学会講演要旨集, 0 (55), 19, 日本語2-3 二次遷移の進行に伴い黒ボク土腐植物質の諸特性はどうなるのか? : 腐植酸の炭素および窒素安定同位体比(2.土壌有機・無機化学,2009年度京都大会)
研究論文（学術雑誌）
- 2009年06月, Pedologist, 53 (1), 46 - 50, 日本語ユーラシアおよびカナダのステップにおけるチェルノーゼム土壌とその腐植物質の特性
[査読有り][招待有り]
Anthraquinones are widely present in plant kingdom, and clinically used as laxatives. Environmental contaminants, dioxins, develop various adverse effects through transformation of a cytosolic aryl hydrocarbon receptor (AhR). We investigated the effects of 18 anthraquinones and 7 of their structurally related compounds on transformation of the AhR estimated by its DNA-binding activity in the cell-free system. 1,4-Dihydroxyanthraquinone (quinizarin), 1,5-dihydroxyanthraquinone (anthrarufin), 1,8-dihydroxyanthraquinone (danthron), and 5-hydroxy-1,4-naphthoquinone (juglone) strongly suppressed DNA-binding activity of the AhR induced by 0.1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), with their IC(50) values around 1 mu M. On the other hand, anthraquinone, 2,6-dihydroxyanthraquinone (anthraflavic acid), and 2-hydroxy-1,4-naphthalendione (lawsone) showed moderate effects. Quantitative structure-activity relationships analysis demonstrated that hydroxyl groups at C1 or C4 but not C3 position of anthraquinone structure are critical for the suppressive effects. In addition, all compounds except lawsone had no agonistic effect. The suppressive effects of anthraquinones in a cultured cell system were also confirmed. In human hepatoma HepG2 cells, chrysophanol, danthron, and rhein also suppressed the DNA-binding activity in a dose-dependent manner, although aloe-emodin showed a moderate effect. The findings of this study may be useful for the design of the novel antagonists of the AhR. (C) 2008, The Society for Biotechnology. Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年03月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 107 (3), 296 - 300, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2009年, 日本土壌肥料学会講演要旨集, 55, 23 - 23, 日本語
- 日本陸水学会, 2009年, 日本陸水学会 講演要旨集, 74, 76 - 76, 日本語
- 2009年, Proceedings of the International Symposium on Soil Organic Matter Dynamics: Land use, Management and Global Change, 英語Effects of ecological succession on the characteristics of soil humic substances in a Japanese volcanic ash soil I. Concentration and chemical properties of humic substances.
研究論文（国際会議プロシーディングス）
- パン酵母β-グルカン摂取によるラットの盲腸内容物および糞便排泄に及ぼす影響
食物繊維不足は消化管疾患をもたらすことが知られている.本研究では,精製したパン酵母由来β-グルカン(BBG)によるラット盲腸内容物および糞排泄に及ぼす影響をセルロース(CE)を対照繊維として用いて比較検討した.BBGまたはCEを1%,3%,5%となるようAIN-93M粉末食に混合し,これらと無繊維食をラットに4週間自由摂取させた.盲腸組織重量は5%BBG食摂取群で5%CE食摂取群と比較して有意に増加し,一方で,盲腸内容物のpHは有意に低下した.ラット盲腸内容物中の酢酸,プロピオン酸,n-酪酸は5%BBG食摂取群で5%CE食摂取群と比較して有意に増加したことから,これらの短鎖脂肪酸の増加が盲腸内容物のpH低下に寄与していることが示唆された.さらに,糞の水分含量も5%BBG食摂取群で5%CE食摂取群と比較して有意に増加したことから,BBGは盲腸内発酵を促進して大腸管腔の水分保持に寄与することが示唆された.以上のことから,BBGは腸機能を調節することで便秘の抑制効果を発揮すること,およびその効果はCEよりすぐれていることが明らかとなった.
日本生物工学会, 2009年, 生物工学会誌, 87 (4), 170 - 174, 日本語[査読有り]
研究論文（学術雑誌）
- パン酵母β-グルカン摂取がラット盲腸内容物および糞便排泄に及ぼす影響
食物繊維不足は消化管疾患をもたらすことが知られている.本研究では,精製したパン酵母由来β-グルカン(BBG)によるラット盲腸内容物および糞排泄に及ぼす影響をセルロース(CE)を対照繊維として用いて比較検討した.BBGまたはCEを1%,3%,5%となるようAIN-93M粉末食に混合し,これらと無繊維食をラットに4週間自由摂取させた.盲腸組織重量は5%BBG食摂取群で5%CE食摂取群と比較して有意に増加し,一方で,盲腸内容物のpHは有意に低下した.ラット盲腸内容物中の酢酸,プロピオン酸,n-酪酸は5%BBG食摂取群で5%CE食摂取群と比較して有意に増加したことから,これらの短鎖脂肪酸の増加が盲腸内容物のpH低下に寄与していることが示唆された.さらに,糞の水分含量も5%BBG食摂取群で5%CE食摂取群と比較して有意に増加したことから,BBGは盲腸内発酵を促進して大腸管腔の水分保持に寄与することが示唆された.以上のことから,BBGは腸機能を調節することで便秘の抑制効果を発揮すること,およびその効果はCEよりすぐれていることが明らかとなった.
日本生物工学会, 2009年, 生物工学会誌, 87 (4), 170 - 174, 日本語[査読有り]
研究論文（学術雑誌）
- 2009年, 緑化工学会誌, 35, 325 - 331, 日本語クリンカアッシュの緑化基盤としての利用が緑化樹木へ及ぼす影響
[査読有り]
研究論文（学術雑誌）
- 2009年, Applied and Environmental Soil Science, 1, 1 - 5, 英語Humus and humic acids of Luvisol and Cambisol of Jiguli ridges, Samara region, Russia
[査読有り]
研究論文（学術雑誌）
- 2008年09月, Proceedings of the 14th meeting of International Humic Substances Society, 2, 503 - 506, 英語Population of humic acid degrading microorganisms under different soil types and vegetation types
[査読有り]
研究論文（国際会議プロシーディングス）
- 2008年09月, Proceedings of the 14th meeting of International Humic Substances Society, 1, 171 - 174, 英語Genesis of Cambisol, Luvisol and Rendzic Leptosol of Samara forest-steppe ridges with special reference to characteristic of humic acids
[査読有り]
研究論文（国際会議プロシーディングス）
- 2008年09月, Proceedings of the 14th meeting of International Humic Substances Society, 1, 333 - 336, 英語Comparison of 13C NMR spectra of fulvic acids from neighboring two clear water lakes in Japan
[査読有り]
研究論文（国際会議プロシーディングス）
To find a certain relation between the composition of carbon functional groups of humic acids derived from liquid state C-13 nuclear magnetic resonance (NMR) spectra acquired with inverse-gated decoupling (IGD), known as a quantitative pulse sequence, and that by solid-state 13C NMR with cross polarization/magic angle spinning (CPMAS) techniques, fifteen soil humic acid samples with a wide range of aromaticity were analyzed. Relationships between the proportions of humic acid carbon as alkyl, O-alkyl, and aromatic carbon assessed by the two methods could be regressed to y=ax (r=0.932-0.956; P<0.005), respectively. The contents of alkyl, O-alkyl, and aromatic carbon assessed by CPMAS method were larger than those found by IGD method. However, the differences between the two methods were small and exclusive regression to y=x was also significant (r=0.775-0.941; P <0.005). Aromaticity calculated from C-13 CPMAS NMR data also did not differ significantly from those computed from C-13 NMR spectra with IGD. These observations indicated the comparability of the relative content of carbon functional groups in humic acids except for carboxyl and carbonyl carbon. (C) 2008 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2008年06月, ANALYTICA CHIMICA ACTA, 618 (1), 110 - 115, 英語[査読有り]
研究論文（学術雑誌）
- Optimization of conditions for high-performance size-exclusion chromatography of different soil humic acids
A method of high-performance size-exclusion chromatography (HPSEC) for a wide variety of soil humic acids (HAs) was developed. Two types of soil HAs (Cambisol and Andosol HAs), which have substantially different chemical properties, showed different effects of salt and organic solvent concentrations in the eluent on chromatograms. A Shodex OHpak SB-805 HQ column with 10 mM sodium phosphate buffer (pH 7.0) containing 25% of acetonitrile (v/v) was found to be applicable for different HAs, and showed high reproducibility and recovery (87.0 - 94.5%). The Cambisol HA was fractionated into five fractions using an ultrafiltration with different molecular-weight cut-offs. The order of the molecular weights of the five fractions calculated from the HPSEC analysis corresponded to that defined by ultrafiltration. This supported the reliability of the method.
JAPAN SOC ANALYTICAL CHEMISTRY, 2008年05月, ANALYTICAL SCIENCES, 24 (5), 607 - 613, 英語[査読有り]
研究論文（学術雑誌）
To examine the effect of vegetation type and grassland management regimen on the distribution of humus-degrading microorganisms, populations of humic acid-degrading (HAD) bacteria and fungi at three Andosol sites were investigated using the dilution plate method. Each site had three different vegetation types (Eulalia grassland, bamboo grassland, and coniferous plantations). Among the six grassland sites, two were maintained by burning and the others by cutting. HAD microorganisms were found in all soil samples. Low densities and small percentages of HAD bacteria were detected with no significant differences in the number of bacteria found between different vegetation types and grasslands managed in different ways. In contrast, the densities and percentages of HAD fungi differed according to vegetation type and management regimen. Specifically, the percentages of HAD fungi were significantly higher for burned grasslands. At burned sites, the numbers and percentages of HAD bacteria remained at a consistently low level, and no distinct seasonal changes were observed. In contrast, marked seasonal fluctuations in HAD fungi were detected. The percentages of these fungi remained relatively high between April and December. These fluctuations are likely due to the effects of burning on soil microorganisms.
JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE, 2008年, MICROBES AND ENVIRONMENTS, 23 (1), 44 - 48, 英語[査読有り]
研究論文（学術雑誌）
Chemical properties of hydrophobic acid (HoA) fractions in water-soluble organic matter in soil and water are concerned with its interactions with mineral soil surfaces and organic pollutants. In 2004 we examined the seasonal and vertical changes in chemical properties of the HoA fraction in a Cambisol profile and compared these properties with those in the HoA fraction of an adjacent stream (aquatic humic substances) in a temperate forested watershed using high performance size exclusion chromatography (HPSEC) and H-1 and C-13 nuclear magnetic resonance (NMR) spectroscopy. The HoA fractions from Oi, Oe/Oa, A and B horizon soils in summer had lower O-alkyl C proportions than those recorded in samples in other seasons. The proportions of aromatic C in HoA fractions from A and B horizons were highest in summer. These seasonal variations were less significant than variations with soil depth. O-alkyl C proportions in HoA fractions decreased with increasing soil depth from the Oi to the A horizon. The HoA fractions from the B horizon showed a higher alkyl C proportion than samples from other horizons in winter and spring. These changes with soil depth from the Oi to A horizons might result from selective utilization of carbohydrate carbon by microorganisms, whereas those in the B horizon may result from sorption to mineral surfaces. The HoA fractions in the stream were similar in relative molecular weight, distribution of each type of proton and carbon species in HoA fractions from the B horizon, whereas stream HoA fractions collected in summer would be derived from organic horizons. This indicated that vertical changes in the chemical properties of HoA fractions in soil and pathways of water to the stream would largely affect the chemical properties of HoA fractions in the stream.
BLACKWELL PUBLISHING, 2007年10月, SOIL SCIENCE AND PLANT NUTRITION, 53 (5), 551 - 561, 英語[査読有り]
研究論文（学術雑誌）
Carbon isotopes (C-13 and C-14) were used to study the characteristics and origin of humic substances in water samples from Lake Biwa in Japan. Humic substances were isolated from surface lake waters in each season by the XAD extraction method and their carbon isotopic ratios were measured by accelerator mass spectrometry. The Delta C-14 values of Biwa humic and fulvic acids indicate that these substances consist mainly of pre-bomb C-14. There is a negative correlation between Delta C-14 and delta C-13 values for the Biwa fulvic acids. The fulvic acid consists of younger organic materials with lower delta C-13 value in January, but older organics with higher delta C-13 in April and October. The major part of fulvic acid may be supplied from soil solution and/or groundwater in the watershed and their contribution varies with season. (c) 2007 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2007年06月, NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 259 (1), 552 - 557, 英語[査読有り]
研究論文（学術雑誌）
C-14 ages and delta C-13 were examined for sclerotium grains to elucidate the characteristics of these grains distributed in forest soils. The ages of the grains from surface A horizons and buried A horizons were ca 100-200 BP and ca 300-1,200 BP, respectively. In comparison with humic acid extracts, the C-14 ages were in the increasing order: humic acid fraction < humic acid Pg fraction < sclerotium grains. The delta C-13 values for sclerotium grains in surface A horizons and buried A horizons were approximately -31 parts per thousand to -28 parts per thousand, and these values were approximately 2-4 parts per thousand smaller than those of humic acids and soils. The C content of the grains had a tendency to decrease with increasing C-14 ages, while the C content of humic acid was constant with age. The C-14 ages of sclerotium grains indicate the individual age of grain formation, which are more likely to assign closer ages to the beginning of soil forming than the C-14 ages of humic acid. The low delta C-13 values for sclerotium grains have presumably originated from characteristically biological organics, which may be protected from attack in soils because of their structure.
TAYLOR & FRANCIS LTD, 2007年04月, SOIL SCIENCE AND PLANT NUTRITION, 53 (2), 125 - 131, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2007年, 日本土壌肥料学会講演要旨集, 53, 19 - 19, 日本語
- 日本陸水学会, 2007年, 日本陸水学会 講演要旨集, 72, 69 - 69, 日本語
- 2007年, Proceedings of International Symposium on Organic Matter Dynamics in Agro-Ecosystems, 402–403, 英語Aluminum and iron contents of sclerotium grains and their role as persistent organic component in forest soils
研究論文（国際会議プロシーディングス）
- 2007年01月, European Journal of Soil Science, 58 (3), 786 - 793, 英語Characterization of major and trace elements in sclerotium grains
[査読有り]
研究論文（学術雑誌）
- 2006年08月, Humic Substances Research, 3, 1 - 10, 英語
[査読有り]
研究論文（学術雑誌）
To clarify the formation condition of Type A humic acids (HAs) from plant residues and to contribute to reducing CO, emission, three plant residues were incubated for 0, 25, 50, 100 and 160 days with fresh volcanic ash and water at 90 degrees C to produce humic acid like substances (HALS). The HALS of all incubation samples showed a decrease in log (A(400)/A(600)) values and an increase in A(600)/C values with increasing incubation time. When HALS were classified, only rice straw HALS of 160 days (RS160) belonged to Type A, whereas broad leaf HALS of 160 days (BL160) and Japanese cedar sawdust HALS of 160 days (JCSD160) belonged to Type B. For elemental and nuclear magnetic resonance (NMR) analysis, the HALS samples were prepared according to the International Humic Substances Society (IHSS) method. Data from the elemental composition of all HALS showed high contents (45.98 to 56.55) of carbon (C), low (3.19 to 5.16) hydrogen (H), and low (0.33 to 2.36) nitrogen (N). Carbon content increased in RS and BL, and increased or fluctuated to some extent in the JCSD, whereas H and N contents decreased in all samples as incubation progressed. The H/C and O/C ratios showed a marked decrease, simultaneously the O/H ratio increased, with increased incubation days. These results suggested that oxidation occurred in the incubation system. Although some HALS samples (RS160 and BL160) appeared in the Type A region in both H/C versus O/C and H/C versus O/H diagrams. HALS plotted in the figures of log (A(400)/A(600)) versus A(600)/C, H/C versus O/C and H/C versus O/H diagrams were generally placed out of the area of natural soil HAs. In the case of 1H NMR, the spectral shape of RS, BL and JCSD differed from that of natural soil HAs. Only the 1H NMR spectrum of RS160 was similar to that of Type A soil HA. Changes in the composition of proton species in HALS showed that percentages of aromatic protons (H-ar) increased with increasing incubation. Spectra obtained using C-13 NMR revealed that RS and BL HALS were different from natural soil HAs, whereas RS160 was similar to Type A HA spectra. In the spectra of RS HALS, the signal intensities of carbohydrate C (approximately 74 and 105 p.p.m.) and methoxyl C (approximately 56 p.p.m.) decreased gradually as incubation time progressed, while carboxylic C (approximately 175 p.p.m.) increased. Simultaneously other distinct signals became broad and overlapped each other. As a result, the changes in RS HALS spectra suggested that progress in the modification of lignin and the oxidative degradation of polysaccharide structure, and the spectrum of RS160 became similar to the spectra of Type A HAs. Although the only signal resulting from phenolic C (approximately 150 p.p.m.) still appeared in RS160, the signal intensity decreased and became broader and weaker from RS0 to RS160 with incubation time. Therefore, it was concluded that RS HALS might form a completely similar spectra to the spectra of natural Type A HAs with longer incubation time.
BLACKWELL PUBLISHING, 2006年06月, SOIL SCIENCE AND PLANT NUTRITION, 52 (3), 349 - 360, 英語[査読有り]
研究論文（学術雑誌）
The chemical composition of the hydrophobic acid fraction, which is adsorbed to XAD-8 resin at low pH and desorbed with high pH solution, of water-extractable organic matter in soil probably influences its environmental role and, therefore, it is important to recognize the differences in its chemical composition with season and soil horizon. Hydrophobic acid fractions were collected seasonally in 2004 from Oi, Oe/Oa, A and B horizons of Cambisol and were investigated using elemental analysis, high-performance size exclusion chromatography and H-1 nuclear magnetic resonance (NMR) spectroscopy. Atomic ratios of H/C and O/C decreased with increasing soil depth in winter and summer, suggesting that the hydrophobic acid fraction from lower mineral horizons had lower saturated bond content and carbohydrate content than the fractions from upper organic horizons. Based on high-performance size exclusion chromatography, the molecular weight at peak maximum of the hydrophobic acid fraction decreased significantly with increasing soil depth. The H-1 NMR spectra of the hydrophobic acid fraction showed remarkable changes. The signal intensities of carbohydrate protons (delta 3.0-4.3 p.p.m.) in the hydrophobic acid fraction from organic horizons decreased from winter to summer and increased in autumn, while the signal of aliphatic protons (delta 0-3.0 p.p.m.) increased and decreased. In all seasons of the year, the signal intensities of carbohydrate protons were greater in organic horizons and decreased significantly with depth in mineral soil, but the signal for aliphatic protons increased. Our results demonstrate that season and soil depth influence the chemical composition of the hydrophobic acid fraction of water-extractable organic
BLACKWELL PUBLISHING, 2006年06月, SOIL SCIENCE AND PLANT NUTRITION, 52 (3), 361 - 370, 英語[査読有り]
研究論文（学術雑誌）
- Effect of Fe, Mn or Al compounds on humification of three types of plant residues during thermal incubation
The effect of three inorganic minerals on the humification of three types of plant residues was determined by employing a model thermal incubation experiment. The plant residues consisiting of rice (Oryza sativa) straw, broadleaf tree (a mixture of oak/beech, Quercus serrata, Q. dentata, Q. acutissima etc.) sawdust and Japanese cedar (Cryptomeria japonica) sawdust were each mixed with Fe, Mn and Al in the form of hydroxides, oxides and sulfates. Humic materials were extracted after incubation and their composition was analyzed using a mixed solution of 0.02 M Na4P2O7 and 0.1 m NaOH. The pH values of the samples after a longer duration of the incubation period were all less than 5.0, with the lowest value of 2.16 for a sample incubated with Al-2(SO4)(3), except for the values of the samples incubated with MnO2, which ranged from 4.75 to 6.0. The Delta logK values decreased with the increase of the duration of the incubation period, whereas the RF values increased, as well as the amount of humus extracted (HE) and percentage of humic acid (PQ). Whereas most of the samples were identified as Type B and Type Rp humic acids, Type A humic acid was formed in all the plant residues incubated with Al-2(SO4)(3), FeO(OH) and MnO2 after <= 180 d of incubation period. Moreover, the degree of humification of the plant residues was observed in the order of broadleaf tree>rice straw>Japanese cedar. It can be concluded that the inorganic compounds Al-2(SO4)(3), FeO(OH) and MnO2 contributed to the acceleration of the humification process of plant residues during the thermal incubation. The effect of Al-2(SO4)(3) may be associated with the increase in the reactivity with other components in the system due to its high solubility, whereas FeO(OH) and MnO2 may be involved in a reduction-oxidation reaction during the incubation. The browning and/or blackening of the plant residues were similar to the production of melanoidin which led us to consider that the mechanism involved in the study was similar to that of the Maillard reaction.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 2005年12月, SOIL SCIENCE AND PLANT NUTRITION, 51 (7), 925 - 933, 英語[査読有り]
研究論文（学術雑誌）
- Method for estimating the content of the chloroform-extractable green fraction (CEGF) in HCl-DMSO extract of soils
Chloroform-extractable green fraction (CEGF) was detected in the supernatant obtained by alkali precipitation from the HCl-dimethylsulfoxide (DMSO) extract of Pg-rich soil. In the alkaline solution, the color of CEGF was green and CEGF showed strong Pg-like absorption bands. Ultraviolet and visible (UV-VIS) spectral analysis and gel chromatography on Sephadex G-50 were performed to compare several properties between CEGF and Pg. CEGF, which was purified by gel chromatography on Bio-Beads SX-1, displayed strong absorption bands at 609, 562, 445 and 280 nm in the alkaline solution. These absorption bands were almost similar to those of Pg. Furthermore, the UV-VIS spectrum of CEGF in the organic solvents showed a similar characteristic pattern of 4,9-dihydroxyperylene-3,10-quinone (DHPQ), which was considered to be a chromophore of Pg. Based on the results of gel chromatography on Sephadex G-50, CEGF mainly consisted of two fractions, corresponding to the G(2) and G(3) fractions of Pg. These results indicated that the method for extracting CEGF from Pg-rich soil in the present study was easy and selective and that CEGF was one of the components of, or a closely related substance to Pg. A colorimetric method for the estimation of the CEGF content in soils was developed. The calibration curve of CEGF was linear over a wide range of contents from 2.75 to 220 mg L-1. The CEGF content in twelve samples of various soils was examined. CEGF was detected in all the soil sampled (5 orders) including three samples (3 orders) where Pg was not detected, and the content ranged from 0.07 to 1.66 g kg(-1) (dry soil). Therefore, the method for estimating the CEGF content in soils developed in the present study was found to be suitable for various soil orders and it was assumed that CEGF occurred in various soil orders.
TAYLOR & FRANCIS LTD, 2005年10月, SOIL SCIENCE AND PLANT NUTRITION, 51 (6), 779 - 786, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2005年, 日本土壌肥料学会講演要旨集, 51, 20 - 20, 日本語
Relationships between the distribution of fungal sclerotium grains and soil chemical properties were studied in forest soils of Podsole, Braunfahlerde, and Braunerde-Podsoles in Harz Mts., Germany. Development of sclerotium grains was dominant in surface horizons (Ah, E horizons) within a 10-cm depth and weight density of grains ranged from 0.01 to 4.99 g kg(-1) soil. The SEM-EDX analysis proved that the weight percentage (excluding C and N) of Al2O3 was 39.8-63.9% inside the grains. The content of elgosterol, a biomarker of viable fungal biomass, showed good correlations with weight density of sclerotium grains in grain-detected soils. The sclerotium grains were likely to be formed in soils with high ratios (>0.6) of organic bonding Al (Al-p) to amorphous Al (Al-o), and with high contents of exchangeable Al (Al3+) (>0.54 g kg(-1)). The content and state of active Al were believed to be responsible for the development of sclerotium because sclerotium grains were not detected in acid soils which had lower levels of free colloidal Al (Al-d, Al-o, Al-p). We believed that the intensive clay destruction associated with past lessivage process induced the absence of free colloid Al in such forest soils.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 2004年12月, SOIL SCIENCE AND PLANT NUTRITION, 50 (6), 863 - 870, 英語[査読有り]
研究論文（学術雑誌）
- Aluminum oxyhydroxide polymorphs and some micromorphological characteristics in sclerotium grains
The presence of aluminum oxyhydroxide and its micromorphological characteristics in sclerotium. grains were examined by SEM, TEM, and EDX analyses. A spherical fraction with a diameter of 100 nm, which contained C and Al as the predominant elements, was recognized as the unit particle of the matrix of sclerotium grains. The Al polymorphs and the Si spherical structures found in the ignited grains were assumed to correspond to boehmite and opaline silica particles, respectively. SEM analysis revealed the presence of a needle ball structure inside the hollow part of sclerotium grains. The relationship between the inner structure of sclerotium and biochemical processes of the host fungi was discussed.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 2004年12月, SOIL SCIENCE AND PLANT NUTRITION, 50 (8), 1205 - 1210, 英語研究論文（学術雑誌）
Swollen chips made from trees felled during clear-cutting were composted with various organic and inorganic materials in an aerobic composting reactor for 5 months and then piled for 5 months. The organic materials included chicken feces, urea, nitrogenous lime (calcium cyanamide, manure), and material rapidly composted from food garbage in 24-h bacterial fermentation, while the inorganic materials were coal ash and volcanic ash. In this paper, we first attempt to estimate the quality and degree of maturity of each compost from its chemical properties. Furthermore, we try to calculate the maturity of the fermented wood chip composts from their mixture ratio of the initial materials by multiple linear regression analysis. We measured changes in the ON ratio, nitrate nitrogen (NO3-N) content, percentage of humic acid in the alkali soluble fraction (PQ),-cation exchange capacity, pH, and EC during the composting period. The degrees of maturity of the composts were estimated via a plant growth test using Chinese cabbage. We found that the CN ratio, NO3-N concentration, and PQ were suitable for estimating the degree of maturity of wood chip composts. For maturity, the CN ratio should be less than 14, the PQ more than 66.2, and the NO3-N concentration more than 853 mg kg(-1). We devised an equation to estimate the degree of maturity after 10 months by a multiple linear regression analysis from the mixing ratio of wood chips and the co-composted materials. From the multiple linear regression analysis, the above three indices of compost maturity could be estimated from the mixing ratio of the initial materials. This equation should enable us to determine the degree of compost maturity after 10 months based on the initial mixing ratio. (C) 2004 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2004年11月, BIORESOURCE TECHNOLOGY, 95 (2), 121 - 128, 英語研究論文（学術雑誌）
Radiocarbon (C-14) was used to study the origin and transport of aquatic humic substances in river waters at the Chernobyl area, which received a pulse input of C-14 as a consequence of the nuclear accident. Water samples were collected in April 1999 from the Pripyat and Sakhan Rivers, which flow through the radioactive contaminated area (30 kkm exclusion zone). The Delta(14)C values of humic and fulvic acids ranged from -68parts per thousand to +75parts per thousand and were similar to400parts per thousand lower than those of non-contaminated environments. The aquatic humic substances may be derived mainly from those of bog, peat, and podzolic soil with older C-14 age, and thereby reflect a larger proportion of older groundwater humic substances. Contribution of C-14 by the Chernobyl accident appears to be small because of the long residence time of organic carbon at the surface soil. (C) 2004 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2004年08月, NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 223, 848 - 853, 英語研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2004年, 日本土壌肥料学会講演要旨集, 50, 18 - 18, 日本語
- 一般社団法人 日本土壌肥料学会, 2004年, 日本土壌肥料学雑誌, 75 (4), 514 - 515, 日本語
- 非アロフェン黒ぼく土における菌核粒子の分布と土壌化学性との関係
Sclerotium grain is the resting body of ectomycorrhizal fungi found in forest soils. A melanic-spherical shape in approximately 1-2mm diameter characterizes the external feature of the grain, and a hollow structure with honeycomb transverse wall appears inside the grain. In our previous studies, we reported a high aluminum concentration inside sclerotium grains and suggested the close relationship between the status of active aluminum and the distribution of these grains in Andosols (Watanabe et al., 2001; 2002). Here we examined the chemical properties focused on active aluminum and carbon in several nonallophanic Andosols under forest vegetation for further discussions on the regulating factor of the distribution of the sclerotium grains. In each studied soil profile, the mean weight of sclerotium grain (mg grain^<-1>) had a tendency to increase with the content of exchangeable aluminum, content of total organic carbon and nitrogen, carbon content of humic acid extracted from soils. The ratio of Alp to total organic C(Al_p/T-C) showed a strong negative correlation between the mean weight sclerotium grains regardless of profiles. The bonding ratio of carbon and aluminum in soils was assumed to be one of the factor influencing the development of sclerotium grains.
日本ペドロジー学会, 2004年, ペドロジスト, 48 (1), 24-32 - 32, 日本語研究論文（学術雑誌）
累積土壌断面内にみられる腐植の安定性を支配する要因を探るために,8,000年前から30,000年前の埋没腐植酸5点について担子菌Coriolus consorsを用いた培養試験を行い,腐植酸褪色率を算出した.3週間培養後の腐植酸培地すべてにおいて対照区に比べて褪色が認められ,8～10%の腐植酸褪色率が示された.これは,従来報告されていた表層土壌中の腐植酸の褪色率に比べると,総じて低い値であった.また,元素分析の結果から,試料として用いた5点の埋没腐植酸は,一様に脱水素反応の進行した類似の化学特性を示すことが明らかとなった.これらのことから,埋没腐植酸の化学特性が微生物作用に対して強い抵抗性を持ち,それが腐植物質の安定性をもたらす一因となり得ることが示唆された.
Japan Association for Quaternary Research, 2004年, 第四紀研究, 43 (5), 367 - 373, 日本語- 2004年, ペトロジスト, 48,24-32, 日本語非アロフェン黒ぼく土における土壌菌核粒子分布と土壌科学性状
研究論文（学術雑誌）
To obtain fundamental information on the nature and properties of sediments in dammed lakes, we investigated various properties of sediment from dammed Lake Ono using soil scientific methods. Furthermore, the Lake Ono sediment was characterized by comparing its various properties with those of yellow soil. 1) Lake Ono is a eutrophic lake which phosphorus is a limiting factor. 2) The pH, exchangeable base content, and degree of base saturation of the sediment were much higher than those of the soil. 3) The clay content of the sediment was 2 times higher than that of the soil. The texture of the sediment was Heavy clay (HC), while that of the soil was Clay loam (CL). There were no large differences on organic carbon and total nitrogen contents, and the C/N ratio between the sediment and the soil. 5) The "combine"-form humus content of the sediment was higher than that of the soil. The reverse was true for the "free"-form humus content. 6) The X-ray diffraction patterns of the clay fraction of the sediment were almost the same as those of the clay fraction of the soil. 7) The elemental composition and 13C-NMR spectrum of the fulvic acid isolated from the sediment were similar to those of the soil. On the basis of the present findings and previous information on the properties of soil distributed in catchment areas, it was assumed that the Lake Ono sediment is strongly affected by soil particles and Ca in its catchment areas, which flow into the lake.
公益社団法人 日本水環境学会, 2004年, 水環境学会誌, 27,9, 597-603 (9), 597 - 603, 日本語研究論文（学術雑誌）
- 2004年, 第四紀研究, 43,367-373, 日本語生成年代の異なる埋没腐植酸の担子菌Coriolus consorsによる褪色特性－愛鷹ロームを事例にして－
研究論文（学術雑誌）
石炭火力発電所から発生する産業廃棄物としての石炭灰の有効利用と,A型腐植酸様物質として二酸化炭素を土壌中に長期固定することを目的に,石炭フライアッシュ(CFA)とイナワラ(RS)を高温培養し,A型腐植酸様物質を含む土壌改良資材の作出を試みた.CFAとRSの配合比や培養温度などを変えた各種条件で培養を行い最適な培養条件を検討した.さらに,生成された培養物の化学的性質も調べた結果,以下のようにまとめられた.1)腐植酸様物質の腐植化度は,低温(60, 75℃)より高温(90℃)で培養するほうが顕著に上昇し,腐植化度の上昇には水分補給が不可欠であることが示唆された.2)腐植酸様物質の腐植化度が高かった培養物は,pHが3〜4程度と低いことが共通しており,培養時のpHを低く調整すれば,腐植化の進行を速くできる可能性が考えられた.3)配合比(CFA:RS)が1:1の場合に最もRSから生成した腐植酸様物質の腐植化度が高く,90℃で培養180日後に0.1 mol L^<-1> Na_4P_2O_7, 0.1 mol L^<-1> H_4P_2O_7混液(pH7)で抽出した腐植酸様物質の〓logKは0.686, RFは94.2を示した.これらから,腐植酸様物質はA型に分類され,CFAとRSの混合物を一定条件下で培養することでA型腐植酸様物質が生成した.4)A型腐植酸様物質の生成が認められた試料(CFA:RS=1:1, 1:2)では,培養0日の試料における全炭素含量のうち,それぞれ少なくとも13.6%, 14.6%がA型腐植酸様物質に変化したと推察された.5)培養物は市販の土壌改良材や堆肥と比べ肥料成分の含量が少なく,現実の施用場面ではNやP肥料などとの併用が必要と考えられた.
日本土壌肥料學會, 2004年, 日本土壌肥料学会誌, 75,641-649 (6), 641 - 649, 日本語研究論文（学術雑誌）
- 2004年, Proceedings of the 6th International Symposium on Plant-Soil Interaction at Low pH PSILPH., 未記入, 英語Status of Aluminum and Humus as the Regulating Factor of the Growth of Sclerotium Grain in Forest Soils, Japan.
研究論文（国際会議プロシーディングス）
- 2004年, Humic Substances Research, 1,1,29-37, 29 - 37, 英語Production and several properties of humic acids during decomposition process of charred plant materials in the presence of H2O2.
研究論文（学術雑誌）
- 2004年, Germany. Soil. Sci. and Plant Nutr., 50(6) 863-870, 英語Morphology, Chemical Properties and Distribution of Sclerotium Grains Found in Forest Soils, Harz Mts.
研究論文（学術雑誌）
- Humification of three plant residues with Sakurajima volcanic ash as influenced by phosphate addition and low pH condition
The influence of phosphate addition and low pH condition on humification of three plant residues were determined by performing model thermal incubations of the plant residues rice straw (Oryza sativa), broadleaf sawdust (Kashiwa, Quercus dentate), and coniferous sawdust (Japanese cider, Cryptomeria japonica) with Sakurajima volcanic ash. Four experimental set ups were prepared: (Group 1) plant residues only; (Group 2) plant residues with Sakurajima volcanic ash; (Group 3) plant residues with Sakurajima volcanic ash and Na_2HPO_4; and (Group 4) plant residues with Sakurajima volcanic ash and Na_2HPO_4 with the pH adjusted to 5. Humic acids were extracted using a mixed solution of 0.02 M Na_4P_2O_7 and 0.1 M NaOH. Extractable Fe and Al oxides were determined using three different extraction methods: dithionite-citrate-bicarbonate (DCB), acid-oxalate (TAMM), and Na-pyrophosphate (NaPP). The pH values obtained for Group 1 samples ranged from 2.75 to 6.21; Group 2 samples were from 3.74 to 4.78; Group 3 samples were near neutral or alkaline, ranging from 6.93 to 7.59; and the values for Group 4 samples ranged from 5.38 to 6.28. The ΔlogK values decreased with longer incubation times while the RF values increased. Group 3 samples showed a decrease in RF values compared to Group 2. Type A humic acids were produced only from Group 2 samples derived from rice straw and broad leaf sawdust after about 120 incubation days. The amounts of the Fe and Al from all types of extraction methods decreased in Groups 3 and 4 indicating a possible binding up of these components with phosphate ions. It can be concluded that addition of Na_2HPO_4 increased the pH of the system and at the same time lowers the solubility of Fe and Al present in the Sakurajima volcanic ash. However, adjusting the pH to 5 to maintain lower pH conditions, has slightly increased the solubility of Fe and Al. Therefore, the higher the amount of extractable Fe and Al, the higher the RF values indicating a faster humification process. The relatively small amounts of Fe and Al in higher pH values and their reaction with phosphate rendered these components ineffective in hastening the humification process. Based on the correlation analysis, Al was more effective in hastening the humification process than Fe.
日本ペドロジー学会, 2004年, ペドロジスト, 48 2 72-82 (2), 72 - 82, 英語[査読有り]
研究論文（学術雑誌）
- 2004年, In Humic Substances and Soil and Water Encironment: Proceedings 12th International meeting of IHSS, Sao Pedro, Sao Paulo, Brazil, Editors: L. Martin-Neto et al., 386-388, 英語Characterization of humic substances in variuous soils and waters by 13C NMR spectroscopy.
研究論文（国際会議プロシーディングス）
- 2004年, Int. J. Medic. Mushrooms., 6, pp.57-62, 英語Changes of lentinan contents and glucanase activity in Lentinus edodes stored under controlled atmosphere.
研究論文（学術雑誌）
- 2004年, Int. J.Medic. Mushrooms, 6, 未記入, 英語Changes of lentinan contents and glucanase activity in Lentinus edodes stored under controlled atmosphere
研究論文（学術雑誌）
- 2004年, International Journal of Medicinal Mushrooms., 6,57-62, 英語Changes of lentinan contents and glucanase activity in lentinus edodes(Berk.) Singer (Agricomycetideae) stored under controlled atmosphere.
研究論文（学術雑誌）
- 2004年, In Humic Substances and Soil and Water Encironment: Proceedings 12th International meeting of IHSS, Sao Pedro, Sao Paulo, Brazil, Editors: L. Martin-Neto et al., 425-427, 英語Changes in structural properties of humic substances transformed by enzymatic reeactions.
研究論文（国際会議プロシーディングス）
- Properties of soil humic substances in fractions obtained by sequential extraction with pyrophosphate solutions at different pHs. III. FT-IR and H-1 NMR spectra of humic acids
The FT-IR and H-1 NMR spectra of humic acids, which were obtained from four soils of different types (a Fibric Histosol, Dystric Cambisol, Umbric Andosol, and buried Umbric Andosol) by sequential extraction with pyrophosphate solutions at different pH values (i.e. at pH 3, 5, 7, 9, 11, and 13), were measured. The shape of the IR spectra for each humic acid depended on the differences in the soil origin rather than the pH values. The IR spectra of humic acids in the Histosol and Cambisol showed typical absorption associated with lignin. The humic acids in the Andosol and buried Andosol were characterized by the disappearance of the absorption assigned to aliphatic C-H and the prominence of the absorption attributed to aromatic C=C and COOH groups. The humic acids in the Histosol and Cambisol showed aliphatic properties compared with those in the Andosol and buried Andosol. As the pH values of the extractant increased, the absorption strength of the bands attributed to aliphatic and amide groups increased and those to carboxylic groups decreased. The H-1 NMR spectra were broadly divided into five regions, namely aromatic (H-ar), methoxyl (HC-O), and aliphatic protons alpha(H-alpha), beta(H-beta), and gamma(H-gamma) to aromatic rings, while the proportion of each proton species was estimated. The proportion of H., in all the humic acids, except for the humic acids at pH 3 in the Histosol and the Andosol, decreased with the increase of the pH values. The increase of the ratio of H-beta to H-ar with the increase of the pH values, was more conspicuous in the following order: Cambisol > Andosol > Histosol and buried Andosol. Based on the results obtained in this study and the results from repeated analysis of the particle size distribution by gel permeation chromatography conducted in Fujitake et al. (Soil Sci. Plant Nutr., 44: 253-260, 1998), a model representation of the relationship between the proton composition and particle size in taking account of the differences in the properties of humic acids among soil types and extraction pH values, was illustrated.
TAYLOR & FRANCIS LTD, 2003年06月, SOIL SCIENCE AND PLANT NUTRITION, 49 (3), 347 - 353, 英語研究論文（学術雑誌）
- Optimization of a methylation procedure to obtain chloroform-soluble humic acids
It will be important in future analyses of humic acids (HAs) by various chromatographic and spectrometric methods to obtain high yields of methylated HAs. A derivatization procedure for permethylation of HAs was evaluated in relation to the yield of the chloroform-soluble fraction. The procedure involved a 3 h incubation of HAs (50 mg) with methylsulfinyl carbanion (MSC) obtained by mixing petroleum-purified NaH with anhydrous dimethylsulfoxide (DMSO), followed by incubation with methyl iodide (CH3I). Ten HAs isolated from different Japanese and American soils (three Andisols, four Inceptisols, one Histosol, one Alfisol, and one Ultisol) were used to optimize this procedure. Optimum derivatization was achieved within the first 6 h after the addition of MSC and CH3I; further incubation (up to 24 h) did not result in significant changes in the yield of the chloroform-soluble fraction (60-100%). For most HAs, the maximal yields of chloroform-soluble fractions were obtained at concentrations of 10 mm for MSC and 13 mm for CH3I.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 2003年06月, SOIL SCIENCE AND PLANT NUTRITION, 49 (3), 453 - 457, 英語研究論文（学術雑誌）
- Relation of chemical properties of soil humic acids to decolorization by white rot fungus-coriolus consors
Humic acids (HAs) from 15 different soil samples were added to Czapek-Dox media inoculated with Coriolus consors IFO 9078. After 14 d incubation, decolorization of these culture media at 600 nm absorbance was measured. The aromaticity, the E-600(1%) value, the total acidity, and the elemental composition of the HAs were also determined, and the relationships between the decolorization and the chemical properties of HAs were examined. Percentage of decolorization of these culture media ranged from 8.8 to 39.5%. Andisol HAs showed a lower percentage of decolorization than the Inceptisol HAs. Percentage of decolorization showed highly negative correlations for the aromaticity, the E-600(1%) value, and the 0 H ratio, and highly positive correlations for the hydrogen content and the H / C ratio. In particular, the highest correlation coefficient (r = -0.836**) was obtained between the aromaticity and the decolorization. In addition, the HAs with a lower decolorization were arranged in the direction of dehydration on the H / C versus O / C diagram. These results showed that HAs with a higher aromaticity and a higher humification degree, showed a higher resistance, to microbial decolorization by C. consors.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 2003年04月, SOIL SCIENCE AND PLANT NUTRITION, 49 (2), 201 - 206, 英語研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2003年, 日本土壌肥料学会講演要旨集, 49, 14 - 14, 日本語
- 一般社団法人 日本土壌肥料学会, 2003年, 日本土壌肥料学会講演要旨集, 49, 16 - 16, 日本語
- 2003年, 土肥誌, 74,223-228, 223 - 228, 日本語我が国の腐植物質研究とその展望 3.腐植物質分析の技術と今後期待される分析手法
研究論文（学術雑誌）
- 2003年, Int. J. Med. Mushrooms, 5, 397-403, 英語Macrophages stimulated with polysaccharide purified from Agaricus brasiliensis enhance mRNA expression of Th1 cytokine including IL-12 and 18
[査読有り]
研究論文（学術雑誌）
- 2003年, Int. J. Medic.Mushrooms, 5,pp.397-403., 383 - 389, 英語Macrophages stimulated by polysaccharide purified from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae) enhance mRNA expression of Th1 cytokineincluding IL-12 and 18
研究論文（学術雑誌）
A small spherical black fungal sclerotium grain from podzolic soils, which was tentatively identified as the resting body of Cenococcum graniforme, was assumed as the source of green polynuclear quinone pigments in P type humic acid (K. Kumada and H.M. Hurst, Nature 214: 631-633, 1967). To examine the presence of bacteria inside sclerotium grains collected from an Andosol profile in Mt. Myoko, central Japan, the grains were repeatedly washed, ultrasonicated and then cultured on diluted nutrient broth. The sum of recovered bacteria as colony-forming units from the wash and ultrasonicate fractions was 1.46×106 (g fresh weight)-1: 88% of the count in the wash fractions (assumably resulting from grain surface and attached soil) and 12% in the ultrasonicate (inside grain). Thirty-one bacterial strains were isolated from the ultrasonicate fraction and their 16S rDNA partial sequences were determined. The predominant group was the Alphaproteobacteria (71%), chiefly the Sphingomonas group (52%). Representative isolates of the Sphingomonas group were examined for their ability to grow on naphthalenesulfonic acids as a model compound of polycyclic aromatic hydrocarbons (PAHs) and also on several phenolic acids. None of the isolates tested utilized the model PAH but many of them used p-hydroxy benzoic, vanillic, p-coumaric and ferulic acids for growth. Based on these results, the relationship between the predominance of Sphingomonas and the chemical character of the sclerotium grain was discussed. © 2003, Japanese Society of Microbial Ecology & The Japanese Society of Soil Microbiology. All rights reserved.
2003年, Microbes and Environments, 18 (3), 126 - 132, 英語研究論文（学術雑誌）
- Association of Am with humic substances isolated from river waters with different water quality
The association properties of Am with aquatic humic substances in a 0.01M NaClO4 solution at pH 6-8 were studied on the basis of molecular size distribution. Ten humic substances isolated from river water with different water quality (pH 3.9-8.0 and dissolved organic carbon (DOC) concentration of 2-40 mg/l) were used for comparing their effects on the association of Am. The molecular size distribution of Am in the presence of humic substances from an uncolored river water (DOC 2 mg/l) was different from that of experimental systems using humic substances from brownish and high DOC (14-40 mg/l) river waters.
KLUWER ACADEMIC PUBL, 2003年, JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 255 (3), 459 - 464, 英語研究論文（学術雑誌）
- 2003年, Bioresouce Technol., 未記入, 英語Aerobic composting of clear-cut tree chips with various co-materials.
研究論文（学術雑誌）
The distribution of sclerotium grains, which are considered to be the resting bodies formed by fungi species, was studied in 5 Andosol profiles in central Japan. Sclerotium grains were detected in the surface A and buried A horizons of nonallophanic (Alp / Alo > 0.5) soils but not in allophanic (Alp / Alo < 0.5) soils. The maximum density of the sclerotium grains in each profile was approximately 2.5 g kg(-1). A highly positive relationship was observed between the mean weight of the sclerotium grains and the content of exchangeable aluminum (Al3+) in soils. Aluminum concentration inside of the sclerotium grains was confirmed by energy dispersive X-ray spectrometer (EDS) analysis. The results obtained suggest that the status of active Al3+ in soils is an important factor for sclerotium. development.
TAYLOR & FRANCIS LTD, 2002年08月, SOIL SCIENCE AND PLANT NUTRITION, 48 (4), 569 - 575, 英語[査読有り]
研究論文（学術雑誌）
- Comparison of decolorization by microorganisms of humic acids with different C-13 NMR properties
Differences in decolorization of five humic acids, added to Czapek-Dox medium and inoculated with Coriolus consors, Coriolus hirsutus, Lenzites betulina, Streptomyces aureofaciens, or an unidentified actinomycete, were investigated. Percent decolorization after incubation for 21 d at 25 degreesC differed among humic acids and microbial strains. In the case of the three fungi, Cambisol humic acids, characterized as having low aromatic C and high carbohydrate C contents, were decolorized to a greater extent (12-51%) than were Histosol humic acids (3-29%) and Andosol humic acids (0-31%). Among humic acids decolorized by C. consors, those with higher percentage decolorization had high contents of carbohydrate C, low contents of aromatic C and a low degree of aromaticity. In contrast, S. aureofaciens decolorized Andosol humic acid 2 (86.5%) and both Cambisol humic acids (40.4-57.2%) but had little effect on Andosol I and Histosol. These results suggested that the resistance of humic acids to microbial decolorization is related to differences in their chemical structural and is microbial species dependent. (C) 2002 Elsevier Science Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2002年05月, SOIL BIOLOGY & BIOCHEMISTRY, 34 (5), 729 - 731, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 2002年, 日本土壌肥料学会講演要旨集, 48, 9 - 9, 日本語
- 一般社団法人 日本土壌肥料学会, 2002年, 日本土壌肥料学会講演要旨集, 48, 10 - 10, 日本語
- 日本土壌微生物学会, 2002年, 土と微生物, 56 (2), 153 - 153, 日本語
- Relationship between viscosity and molecular weight in an Andosol humic acid
Viscosity and molecular weight of particle size fractions obtained from an Andosol humic acid (HA) were determined. Viscosity was determined both in solutions with and without the addition of 0.1 Rr NaCl (Cs 0.1 and Cs 0, respectively) at pH 7.0, Polyelectrolytic character was observed in the particle size fractions based on the changes in the concentration dependence of reduced viscosity with NaCl concentrations. The use of a Cs 0.1 solution was suitable for the determination of the values of intrinsic viscosity ([eta]) of the particle size fractions. The [eta] value increased with increasing weight average molecular weight ((M) over bar (w)) determined by gel permeation chromatography (GPC), and ranged from 4.3 to 12.9 x 10(-3) (L g(-1)) in the Cs 0 solution and from 3.5 to 6.6 x 10-3 (L g(-1)) in the Cs 0.1 solution. A linear relationship between [eta] in the Cs 0.1 solution and (M) over bar (w) on a logarithmic scale, which was similar to the Mark-Houwink equation, was observed. The value of the constant alpha calculated from the relationship, which reflected the polymer morphology, was 0.75 in the Cs 0.1 solution, and it corresponded to a polymer with flexible chains.
TAYLOR & FRANCIS LTD, 2001年06月, SOIL SCIENCE AND PLANT NUTRITION, 47 (2), 399 - 404, 英語[査読有り]
研究論文（学術雑誌）
The morphology and chemical composition of fungal sclerotium-like grains collected from the buried humic horizon of volcanic ash soil in Mt, Myoko were examined using a scanning electron microscope (SEM): energy dispersion Xray micro-analyzer (EDX), and electron probe micro-analyzer (EPMA). A high C content, low level of Si and high concentration of Al characterized the grains considered to correspond to the sclerotia of Cenoccocum graniforme. The Al-27 MAS NMR spectrum of the sclerotia showed the existence of 6- and 4-coordinated Al. X-ray diffraction analyses supported the presence of amorphous Al (ex. Al-humus complex) in the sclerotia.
TAYLOR & FRANCIS LTD, 2001年06月, SOIL SCIENCE AND PLANT NUTRITION, 47 (2), 411 - 418, 英語[査読有り]
研究論文（学術雑誌）
- Studies of complex formation between anthraquinones and metal ions by electrospray ionization mass spectrometry
The metal complexation of the hydroxyanthraquinones, chrysotalunin, chrysophanol and their derivatives, with Al3+, Ni2+ and Zn2+ was studied by electrospray ionization mass spectrometry (ESI-MS). The results showed that chrysophanol and its derivatives form metal complexes with Al3+, Ni2+ and Zn2+ ions, but chrysotalunin does not. Among the metal ions, Al3+ was found to form the complexes most easily.
IM PUBLICATIONS, 2001年, EUROPEAN JOURNAL OF MASS SPECTROMETRY, 7 (6), 467 - 471, 英語[査読有り]
研究論文（学術雑誌）
- C-13 NMR spectra and elemental composition of fractions with different particle sizes from an Andosol humic acid
Elemental composition and solution C-13 NMR spectra for six humic acid fractions with different particle sizes (i.e. 3K, 10K, 30K, 100K, 300K, and 500K) which were prepared from a humic acid in an Umbric Andosol by successive gel permeation chromatography were determined. Elemental composition of the particle size fractions clearly differed from one another. Contents of nitrogen and hydrogen increased with the increase of the particle size. Small and middle particle size fractions (3K to 100K) showed low contents of hydrogen and nitrogen corresponding to those in typical Andosol humic acids, while the contents in the large fractions (300K and 500K) were distributed in the ranges of those in the other humic acids. As the particle size increased, the H/C ratio increased whereas the O/H decreased. In the H/C versus O/C diagram, the large particle size fractions (300K and 500K) were observed in the area around humic acids with a low humification degree. Variation of the chemical structural properties with particle size differences was assumed, based on the analysis of the H/C versus O/H and H/C versus O/C diagrams. The changes of the carbon species with particle size differences were examined based on the C-13 NMR spectra. As the particle size increased, the aliphatic C content increased whereas the carboxylic and aromatic C content decreased and, therefore, the aromaticity decreased. In addition, a negative correlation (r=-0.977) was found to be significant at 0.1% level between the H/C ratios and aromaticity. The peak strength around 30 ppm assigned to chains of methylene group increased remarkably with the increase of the particle size. It was suggested that the changes of the content of the aliphatic carbon with the decrease of the particle size may depend on the changes of the aliphatic chain length. In conclusion, these findings suggest that the Andosol humic acid molecule may be composed of humic acid components with long chains of aliphatic groups for the larger particle size fractions, and of their homologous series through the reduction of the length of the aliphatic long chain for the smaller particle size fractions. These assumptions were compatible with those made in a series of studies previously reported.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1999年06月, SOIL SCIENCE AND PLANT NUTRITION, 45 (2), 359 - 366, 英語[査読有り]
研究論文（学術雑誌）
- Properties of soil humic substances in fractions obtained by sequential extraction with pyrophosphate solutions at different pHs - II. Elemental composition and UV-VIS spectra of humic acids
Humic acids (HAs) obtained from four different soils by sequential extraction with pyrophosphate solutions at different pH values (i.e. at pH 3, 5, 7, 9, 11, and 13), were examined by ultraviolet and visible (UV-VIS) spectroscopy and elemental analysis. UV-VIS spectra from 230 to 700 nm were measured, and absorbance coefficients of 1% humic acid solutions at 600 nm (E-600(1%)) and Delta log K were calculated. The shape of the spectra of humic acids depended on the soil types rather than on the pH values. The E-600(1%) values were higher in the neutral region, and lower in the alkaline region, for each pH value they decreased in the following order: buried Andosol > Andosol > Histosol greater than or equal to Cambisol. The Delta log K values for each pH value were higher in the following order: Histosol > Cambisol > buried Andosol greater than or equal to Andosol. Although the linear correlation was found to be significant between the E-600(1%)) and Delta log K values, the relation between these optical properties and pH values differed among the soil types. However, in the neutral region, the higher E-600(1%) values of humic acids from buried Andosol and Andosol and the lower Delta log K values of humic acids from Cambisol and Histosol suggest that the humification degree was higher in the neutral region. Data of elemental composition and atomic ratios were obtained for each humic acid. The H/C ratios in the four soils increased gradually with increasing pH values from 5 to 13, and those for each pH value were commonly higher in the following order: Cambisol > Histosol > Andosol > buried Andosol. The O/C and O/H ratios decreased with increasing pH values. The results of the H/C versus O/C diagram suggested that decarboxylated humic acids were extracted at the higher pH values. A highly significant correlation coefficient was obtained between the H/C and O/H ratios (r = -0.891***), Results showing that the decrease of the pH values corresponded to the order of the humic acids in the correlation curve in the H/C versus O/H diagram suggest that the humic acids with a higher content of unsaturated bonds and a higher degree of oxidation were extracted at the lower pH values within the range from 5 to,13.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1999年06月, SOIL SCIENCE AND PLANT NUTRITION, 45 (2), 349 - 358, 英語[査読有り]
研究論文（学術雑誌）
- Horizontal distribution of main hydroxyanthraquinones in soil
Six hydroxyanthraquinones (chrysophanol, chrysotalunin, microcarpin, physcion, 7,7'-biphyscion, and hinakurin) present in the samples of 26 surface soils were quantitatively analyzed, and the contents of HAQs in soil types were compared. The soil samples had been collected from 19 Umbric Andosols and seven Distric Cambisols, and the Andosols were further subdivided into those with allophanic soil materials (exchange acidity (y(1))<5 mL 100 g(-1)) and non-allophanic soil materials (y(1)greater than or equal to 5 mt 100 g(-1)). The following results were obtained. (1) It was determined quantitatively for the first time that chrysotalunin was the major hydroxyanthraquinone in many soils. (2) The amounts of major dimeric hydroxyanthraquinones (chrysotalunin, 7,7'-biphyscion, and microcarpin) in non-allophanic soil materials were significantly larger than those in allophanic soil materials. As the contents of chrysotalunin in Andosols were positively correlated with y(1), which was reported to be positively correlated with aluminum toxicity and exchangeable aluminum in soil, it is suggested that toxic aluminum may be involved in the production of soil hydroxyanthraquinones.
TAYLOR & FRANCIS LTD, 1999年06月, SOIL SCIENCE AND PLANT NUTRITION, 45 (2), 297 - 306, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人日本土壌肥料学会, 1999年, 日本土壌肥料学会講演要旨集, 45, 234 - 234, 日本語
- 一般社団法人日本土壌肥料学会, 1999年, 日本土壌肥料学会講演要旨集, 45, 234 - 234, 日本語
- 一般社団法人日本土壌肥料学会, 1999年, 日本土壌肥料学会講演要旨集, 45, 19 - 19, 日本語
The vertical distribution of main soil hydroxyanthraquinones (HAQs) in the soil profiles of five Japanese Andosols, one Japanese Gambisol, and one Nepalese Gambisol was analyzed quantitatively, using high performance liquid chromatography and thin Bayer chromatography with a two stage development accompanied by scanning densitormetry. The results were as follows: The vertical distribution patterns of chrysophanol (CIPL) and its dimers in Andosols profiles shewed maximum contents in the middle past of the surface horizon. The vertical distribution of physcion (PYS) and its dimers in soil profiles showed various patterns in each profile. AdditionaBPy, we investigated the vertical distribution of HAQs in AndosoPs under a Miscanthus sinensis grassland, and in an adjacent successional red pine (Pinus densiflora) forest at Sugadaira, Central Japan. En the surface soil (0–15 cm), the contents of the dimers of CPL (microcarpin and chrysotalunin) slightly increased and those of the dimers of PPS (7,7′-biphyscion and Rinakurian) showed a remarkable increase in the succession, whereas the contents of monomeric HAQs (CBL and PYS) slightly decreased suggesting preferential production of dimeric HAQs below a depth of 15 em in the forest Andosol. The dimers of CPL and the dimers sf PPS exhibited diRerent structures, vertical distribution patterns, and changes in the contents in Andosols in the secondary succession. Therefore, it was assumed that dimeric reaction processes of CPL may be different from those of PYS, if dimeric reactions had occurred in soil. © 1999, Taylor & Francis Group, LLC.
1999年, Soil Science and Plant Nutrition, 45 (3), 551 - 561, 英語[査読有り]
研究論文（学術雑誌）
- Surface-active properties of particle size fractions in two humic acids
The relationships between surface active properties and humic acid (HA) particle sizes were investigated. Two HAs from an Ando soil and a Brown forest soil were separated into 6 particle size fractions by gel permeation chromatography. Surface-active properties characterized by surface excess value (Gamma mol cm(-2)), cross-sectional surface area per molecule (A nm(2)), critical micelle concentration (CMC g L-1), efficiency and effectiveness of water surface tension reduction were obtained by the measurement of the surface tension of HA solutions from different particle size fractions. For the HA from the Ando soil, except for the smallest particle size fraction, increasing particle size enhanced the efficiency of reduction of the water surface tension and decreased the CMC, while the effectiveness of reduction of the water surface tension was about the same. The surface activity of the HA from the Ando soil increased with increasing particle size. This phenomenon was similar to the surface activity of a homologous series of surfactants, which increased with increasing alkyl chain length. For the HA from the Brown forest soil, the smallest particle size fraction and three large fractions showed a high efficiency, namely a high surface activity. The smallest fraction from the Brown forest soil showed the highest efficiency and the lowest CMC value. In both HAs, the smallest particle size fraction showed exceptional surface-active properties compared with the other fractions and three fractions with large particle size showed a higher surface activity than other smaller fractions.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1998年12月, SOIL SCIENCE AND PLANT NUTRITION, 44 (4), 497 - 505, 英語[査読有り]
研究論文（学術雑誌）
- Properties of soil humic substances in fractions obtained by sequential extraction with pyrophosphate solutions at different pHs I. Yield and particle size distribution
Humic (HAs) and fulvic acids (FAs) were obtained from A horizons of four different soils (a Fibric Histosol, Dystric Cambisol, Umbric Andosol, and buried Umbric Andosol) by the sequential extraction with pyrophosphate solutions at different pHs (i.e. at pH 3, 5, 7, 9, 11, and 13). The yield of the FAs from the four different soils decreased with the increase in pH values from 3 to 7, and then, increased or remained constant with pH values from 9 to 13. The distribution pattern of the yield of HAs differed among the four soils, and the total yield for HAs extracted at pH values extending from the acid to neutral region (3-7) was equal to or higher than that in the alkaline region (9-13), except for the Histosol. In particular, the yield of buried Andosol HAs at pH values of 3, 5, and 7 was remarkably high, ca. 25-30% of total HAs with pyrophosphate extractants. Sequential extraction with the pyrophosphate solutions gave sufficient amounts of HAs extracted for wide-ranging pH values extending from the acid to alkaline region to compare the properties of individual HAs based on the difference in pH values. Particle size distribution analysis of each HA determined by gel permeation chromatography (GPC) suggested that the proportion of components with a larger particle size differed among the HAs extracted from the four soils for the same pH value. It is, however, interesting to note that the proportion of components of HAs with a larger particle size in the four different soils increased gradually and regularly with the increase of the pH value. These results suggest that this sequential extraction technique is suitable for obtaining a series of HAs differing in particle size.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1998年06月, SOIL SCIENCE AND PLANT NUTRITION, 44 (2), 253 - 260, 英語[査読有り]
研究論文（学術雑誌）
- Predomination of dimers over naturally occurring anthraquinones in soil
Four bianthraquinones and two monoanthraquinones were isolated as the major soil anthraquinones from a volcanic ash soil in Japan. They were identified as a new natural product 5,5'-biphyscion (named hinakurin) (3) and five known compounds, chrysotalunin (1), (-)-7,7'biphyscion (2), microcarpin (4), chrysophanol (5), and physcion (6) using MS, 1D NMR, and 2D NMR techniques. Although the dimers (1-4) are rarely found as natural products, they, along with 5 and 6, were ubiquitous and predominant over other anthraquinones in various soils from Japan and Nepal.
AMER CHEMICAL SOC, 1998年02月, JOURNAL OF NATURAL PRODUCTS, 61 (2), 189 - 192, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人日本土壌肥料学会, 1998年, 日本土壌肥料学会講演要旨集, 44, 193 - 193, 日本語
- 一般社団法人日本土壌肥料学会, 1998年, 日本土壌肥料学会講演要旨集, 44, 192 - 192, 日本語
- 一般社団法人日本土壌肥料学会, 1998年, 日本土壌肥料学会講演要旨集, 44, 14 - 14, 日本語
- 一般社団法人日本土壌肥料学会, 1998年, 日本土壌肥料学会講演要旨集, 44, 14 - 14, 日本語
- 一般社団法人日本土壌肥料学会, 1998年, 日本土壌肥料学会講演要旨集, 44, 15 - 15, 日本語
- 一般社団法人日本土壌肥料学会, 1998年, 日本土壌肥料学会講演要旨集, 44, 16 - 16, 日本語
- 一般社団法人日本土壌肥料学会, 1997年, 日本土壌肥料学会講演要旨集, 43, 13 - 13, 日本語
- 一般社団法人日本土壌肥料学会, 1997年, 日本土壌肥料学会講演要旨集, 43, 14 - 14, 日本語
- 一般社団法人日本土壌肥料学会, 1997年, 日本土壌肥料学会講演要旨集, 43, 13 - 13, 日本語
- 一般社団法人日本土壌肥料学会, 1997年, 日本土壌肥料学会講演要旨集, 43, 15 - 15, 日本語
- 一般社団法人日本土壌肥料学会, 1997年, 日本土壌肥料学会講演要旨集, 43, 14 - 14, 日本語
- 一般社団法人 日本土壌肥料学会, 1997年, 日本土壌肥料学雑誌, 68 (3), 245 - 246, 日本語
- 一般社団法人日本土壌肥料学会, 1997年, 日本土壌肥料学会講演要旨集, 43, 110 - 110, 日本語
- Structural information obtained from spectral analysis (UV-VIS, IR, H-1 NMR) of particle size fractions in two humic acids
Humic acids (HAs) extracted from an Umbric Andosol (UA) and a Dystric Cambisol (DC) were fractionated into 7 fractions by successive gel permeation chromatography (GPC) and their fractions were studied by spectral analysis. With increasing particle sizes, the absorption of methylene groups and amide of peptides increased in the IR spectra of both HAs and that of carboxylic groups decreased in the IR spectra of UA, the HA obtained from an Umkric Andosol. Based on the H-1 NMR data in UA, there was a significantly negative linear correlation (-0.950, p<0.001) between H-ar (delta 6.0-9.0 ppm) and H-c-o (delta 3.0-4.3 ppm). Such a negative linear correlation (-0.868, p<0.01) was also observed between H-ar and H-beta (delta 0.9-1.6 ppm). In addition, with increasing particle sizes, the percentage of aromatic protons decreased (from 30.6 to 4.2%). These results suggest that the increase in HA particle sizes depends on the length of aliphatic groups and the bond of methoxyl groups, and HAs were composed of HA molecules of homologous series. On the other hand, in DC, HA obtained from Dystric Cambisol, no significant relationships were observed between each type of protons and aromatic protons. It is suggested that a mixture of non-humic substances smeared the spectral data of DC.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1996年06月, SOIL SCIENCE AND PLANT NUTRITION, 42 (2), 355 - 360, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人 日本土壌肥料学会, 1996年, 日本土壌肥料学雑誌, 67 (4), 460 - 461, 日本語
- Quantitative determination of the main hydroxyanthraquinones in soils by TLC with two-stage development
The separation and quantitation of the five main hydroxyanthraquinones in soils has been performed by TLC with two development steps and densitometric scanning. The five hydroxyanthraquinones were completely resolved both from each other and from other accompanying compounds and were quantified hy densitometry in reflectance mode at a sample wavelength of 445 nm and a reference wavelength of 540 nm. The detection limit of the method was in the lower nanogram range (5-10 ng) and the peak area response was linearly dependent on quantity in the range 1-300 ng.
SPRINGER HUNGARICA KIADO KFT, 1996年01月, JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 9 (1), 48 - 51, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人日本土壌肥料学会, 1995年, 日本土壌肥料学会講演要旨集, 41, 13 - 13, 日本語
- 一般社団法人日本土壌肥料学会, 1995年, 日本土壌肥料学会講演要旨集, 41, 16 - 16, 日本語
- 一般社団法人日本土壌肥料学会, 1995年, 日本土壌肥料学会講演要旨集, 41, 123 - 123, 日本語
- 一般社団法人日本土壌肥料学会, 1995年, 日本土壌肥料学会講演要旨集, 41, 124 - 124, 日本語
- 一般社団法人日本土壌肥料学会, 1994年, 日本土壌肥料学会講演要旨集, 40, 15 - 15, 日本語
- 一般社団法人日本土壌肥料学会, 1994年, 日本土壌肥料学会講演要旨集, 40, 15 - 15, 日本語
- 一般社団法人日本土壌肥料学会, 1993年, 日本土壌肥料学会講演要旨集, 39, 336 - 336, 日本語
The distribution of CLN (a dimer of chrysophanol), which is a common pigment, was determined by HPLC in a variety of soils from widely separated locations in Japan: (1) CLN had a widespread distribution among Japanese soils (2) in soils of A-type humic acid (HA), CLN contents were higher than those in soils of other types (3) for an ando soil CLN was mainly found in the HA and humin (HM) fractions rather than in fulvic acid (4) it is suggested that the distribution of CLN in this profile depends on that in the HA fractions (5) distribution of CLN in HN fractions and its presence in buried humus horizon (6400-10200 YBP) suggests its high stability. © 1992.
1992年05月30日, Science of the Total Environment, The, 117-118 (C), 219 - 226, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人日本土壌肥料学会, 1992年, 日本土壌肥料学会講演要旨集, 38, 14 - 14, 日本語
- 一般社団法人日本土壌肥料学会, 1992年, 日本土壌肥料学会講演要旨集, 38, 14 - 14, 日本語
- DISTRIBUTION OF CHRYSOTALUNIN, A MAJOR SOIL ANTHRAQUINONE PIGMENT, IN VARIOUS SURFACE SOILS OF JAPAN
The contents of chrysotalunin (CLN) in Japanese surface soils were first measured by isolative determination using high performance liquid chromatography (HPLC). These soil samples (N = 67) which consist of red soils, yellow soils, dry podzolic soils, brown forest soils, black soils, and immature soils, were taken from various locations in Japan extending from the southern subtropical to the northern subarctic regions. All the soil samples (N = 67) gave CLN contents ranging from 0.1 to 29.1 ppm. The average CLN content of the black soils which was 10.2 (+/- 1.4, S.E.) ppm (N = 36), was about two times as high as that of the brown forest soils (4.8 +/- 1.2 ppm, N = 19). A significant difference was found in the CLN contents among black soils, brown forest soils, and the soils belonging to other types (p < 0.05). Although no relationship between the CLN contents and soil properties (cf. soil pH, organic carbon contents, Fe and Al contents, etc.) was observed, an obvious difference in the frequency distributions of log-transformed CLN contents was detected among the soil groups under different land uses, for example grasslands with bamboo grass (Sasa sp. or Neosasamorpha sp.) and/or eulalia (Miscanthus sinensis) (19.0 +/- 2.6 ppm, N = 10), forests with bamboo grass and/or eulalia (9.9 +/- 1.4 ppm, N = 15), paddy fields (1.8 +/- 0.1 ppm, N = 5), etc.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1991年12月, SOIL SCIENCE AND PLANT NUTRITION, 37 (4), 619 - 627, 英語[査読有り]
研究論文（学術雑誌）
- DETERMINATION OF CHRYSOTALUNIN, A PREDOMINANT SOIL ANTHRAQUINONE PIGMENT, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
A high-performance liquid chromatographic method for the determination of chrysotalunin (1,1', 8,8'-tetrahydroxy-3,3'-dimethyl-7,7'-bianthraquinoyl), the main hydroxyanthraquinone pigment in soils, is proposed. This method is based on the acetylation of chrysotalunin, and separation and determination of its tetraacetate by liquid chromatography. The minimum detectable amount of chrysotalunin expressed as chrysotalunin acetate was 10 pg. Seventeen samples of different soil types in Japan containing various amounts of carbon were examined. Chrysotalunin amounts ranged from 0.5 ppm (Orthic Acrisols) to 27.7 ppm (Humic Andosols). In addition, for comparison, all samples were assayed using a colorimetric method. From the results of these analyses, the chromatographic method was found to be suitable for the determination of chrysotalunin in soils of various types.
ELSEVIER SCIENCE BV, 1991年04月, GEODERMA, 48 (1-2), 83 - 91, 英語[査読有り]
研究論文（学術雑誌）
- 一般社団法人日本土壌肥料学会, 1989年, 日本土壌肥料学会講演要旨集, 35, 16 - 16, 日本語
- 一般社団法人日本土壌肥料学会, 1988年, 日本土壌肥料学会講演要旨集, 34, 9 - 9, 日本語
- 一般社団法人日本土壌肥料学会, 1986年, 日本土壌肥料学会講演要旨集, 32, 18 - 18, 日本語
MISC
- 2021年, 日本土壌肥料学会講演要旨集(Web), 67植生・深度別のマングローブ土壌有機物の貯留量と組成(トラート・タイ王国)
- 2021年, 日本陸水学会大会講演要旨集, 85thMulti-Shot Pyrolizer GC/MSを用いた琵琶湖由来フルボ酸の化学特性解析
- 2020年, 日本土壌肥料学会講演要旨集(Web), 66強酸性土壌における溶存有機物の三次元蛍光分析手法の開発
- 2019年, 日本土壌肥料学会講演要旨集(Web), 65タイ王国トラート川河口マングローブ林における植生・深度別土壌有機物の分光学的特徴
- 2019年, 日本土壌肥料学会講演要旨集(Web), 65南極土壌の有機物および鉱物の特性
- 2018年, 日本土壌肥料学会講演要旨集, 64輪作から単作へ転換された水田土における種々の溶解有機物の施肥処理の下での長期的動態と分光学的特性
- 2018年, 極域科学シンポジウム(Web), 9th南極湖沼における底生群集の光合成の季節的変動
- 2018年, 日本生態学会大会講演要旨(Web), 65th南極湖底堆積物の硝化能定量の試み
- 2018年, 日本生態学会大会講演要旨(Web), 65thタイ王国トラート川流域マングローブ林における溶存無機炭素の日変動:雨季と乾季における観測事例
- 2018年, 日本土壌肥料学会講演要旨集, 64石垣島吹通川マングローブ林の土壌腐植物質の化学構造特性解析
- 2018年, 日本土壌肥料学会講演要旨集, 64異なる植生,地形および地質特徴のマングローブ林内の堆積炭素
- 2018年, 日本土壌肥料学会講演要旨集, 64南極地域宗谷海岸露岩域に土壌はあるのか
- 2018年, 日本陸水学会大会講演要旨集, 83rd南極湖底堆積物の窒素代謝を探る
- 2018年, 日本陸水学会大会講演要旨集, 83rd東南極・宗谷海岸露岩域における溶存有機物の特性評価
- 2018年, 日本陸水学会大会講演要旨集, 83rd南極湖底生物群集の光合成の季節変化,および湖底堆積物による湖沼誕生年代の解明に向けた研究
- 2018年, 極域科学シンポジウム(Web), 9th南極雪鳥沢およびやつで沢の溶存有機物の特性
- 2018年, 日本生態学会大会講演要旨(Web), 65th, ROMBUNNO.P2‐310 (WEB ONLY), 日本語マングローブの生態系純生産量(NEP)は本当に大きいのか?
- 2018年, 日本地球惑星科学連合大会予稿集(Web), 2018, ROMBUNNO.ACG43‐02 (WEB ONLY), 日本語マングローブ林における炭素動態定量のための水・物質動態モデリング
- 2018年, 日本地球惑星科学連合大会予稿集(Web), 2018, ROMBUNNO.ACG43‐P04 (WEB ONLY), 日本語石垣島吹通川マングローブ林における炭素循環と生態系純生産量
- 2017年, 日本土壌肥料学会講演要旨集, 63畜種・副資材が異なる堆肥中水溶性有機物の特性
- 2017年, 極域科学シンポジウム(Web), 8th南極の発達したコケ群落下の土壌は明瞭な硝化活性を有する
- 2017年, 極域科学シンポジウム(Web), 8th南極湖沼溶存有機物の化学的多様性
- 公益財団法人日本学術協力財団, 2016年02月, 学術の動向, 21 (2), 49 - 53, 日本語土・水環境に遍在するフミン物質の構造化学的特徴とその多様性
[招待有り]
記事・総説・解説・論説等（学術雑誌）
- 2016年, 日本土壌肥料学会講演要旨集, 62堆肥中の水溶性腐植物質抽出条件の検討
- 2016年, 日本土壌肥料学会講演要旨集, 62マングローブ林内土壌への海水塩によるフミン酸の選択的蓄積
- 2016年, 日本陸水学会大会講演要旨集, 81st (Web)フミン物質分別定量法および光学的手法を用いた溶存有機物の特性評価~タイ王国トラート川における事例~
- 一般社団法人 日本土壌肥料学会, 2016年, 日本土壌肥料学会講演要旨集, 62 (0), 19 - 19, 日本語
- 一般社団法人日本土壌肥料学会, 2015年09月09日, 日本土壌肥料学会講演要旨集, (61), 45 - 45, 日本語P3-2-8 生分解性レジンコンクリートの分解制御方法の検討(ポスター,3-2 土壌生物の応用と制御,2015年度京都大会)
- 一般社団法人日本土壌肥料学会, 2015年09月09日, 日本土壌肥料学会講演要旨集, (61), 151 - 151, 日本語P7-2-5 各種資材の土壌施用による丹波黒の連作障害の軽減方法の検討(ポスター,7-2 土壌改良資材,2015年度京都大会)
- 一般社団法人日本土壌肥料学会, 2015年09月09日, 日本土壌肥料学会講演要旨集, (61), 128 - 128, 日本語7-1-4 腐食酸資材及び無機成分施用が土壌CECに及ぼす影響(第2報)(7-1 肥料および施肥法,2015年度京都大会)
- 一般社団法人日本土壌肥料学会, 2015年09月09日, 日本土壌肥料学会講演要旨集, (61), 127 - 127, 日本語7-1-1 木質系副資材が家畜ふん堆肥中の有機物質のNMRスペクトル特性に及ぼす影響(7-1 肥料および施肥法,2015年度京都大会)
- 2015年, 日本土壌肥料学会講演要旨集, 61マングローブ林内土壌における脱塩洗浄が溶存有機物の溶出に及ぼす影響
- 2015年, 日本生態学会大会講演要旨(Web), 62nd, PA1-186 (WEB ONLY), 日本語高緯度北極の湿原生態系における溶存有機物の特性
- フィンランド東部の北方林を通過する雨水中溶存有機物の構成分子種の変化について
本研究では，フィンランド東部の北方林を対象に，溶存有機物（DOM）の分子化合物が森林内の水移動を通してどのように変化するかを検討することを目的とした。このため，対象試験流域において林外雨，林内雨，土壌水，地下水，河川水を採取し，フーリエ変換イオンサイクロトロン共鳴型質量分析法（FT-ICR MS）を用いて，試料水中のDOM分子化合物の同定を試みた。FT-ICR MSで同定できたDOM分子化合物の種類の数は865～2194の範囲に及んだ。この結果は各試料水中に多種多様なDOM分子化合物が含まれていることを示す。一方，試料水間で共通するDOM分子化合物はほとんど存在しないことがわかった。このことは，雨水が樹冠や森林土壌，地下水を通過し河川に到達する過程で雨水中のDOM分子化合物の構成が様々に変化することを示唆する。さらに，同一採取場所で得られた試料水であっても採取時期が異なると，共通するDOM分子化合物はほとんど存在しないことがわかった。以上より，北方林におけるDOMの分子構成は時間的・空間的に変化することが考えられた。
日本森林学会, 2015年, 日本森林学会大会発表データベース, 126 (0), 日本語 - 一般社団法人日本土壌肥料学会, 2014年09月09日, 日本土壌肥料学会講演要旨集, (60), 133 - 133, 日本語P7-1-13 木質系副資材が堆肥由来の水溶性腐植物質の量および質的変化に及ぼす影響(ポスター,7-1 肥料および施肥法,2014年度東京大会)
- 一般社団法人日本土壌肥料学会, 2014年09月09日, 日本土壌肥料学会講演要旨集, (60), 152 - 152, 日本語8-1-39 リン酸欠乏と高二酸化炭素濃度条件が2種類のルーピンとトマトの重金属吸収能力に与える影響(8-1 環境保全,2014年度東京大会)
- 一般社団法人日本土壌肥料学会, 2014年09月09日, 日本土壌肥料学会講演要旨集, (60), 25 - 25, 日本語P2-1-4 ドイツBaden-Wurttemberg州の農耕地の腐植酸の化学構造特性に耕作が与える影響(ポスター,2-1 土壌有機・無機成分の構造・機能・ダイナミクス,2014年度東京大会)
- 一般社団法人日本土壌肥料学会, 2014年09月09日, 日本土壌肥料学会講演要旨集, (60), 119 - 119, 日本語7-1-11 腐植酸資材及び無機成分施用が土壌CECに及ぼす影響(7-1 肥料および施肥法,2014年度東京大会)
- 一般社団法人日本土壌肥料学会, 2014年09月09日, 日本土壌肥料学会講演要旨集, (60), 17 - 17, 日本語2-1-6 C/N比の異なる堆肥の連用が土壌腐植酸の量的変化に及ぼす影響(2-1 土壌有機・無機成分の構造・機能・ダイナミクス,2014年度東京大会)
- 2014年, 日本陸水学会大会講演要旨集, 79th (Web)琵琶湖における新たな有機物管理の考え方について
- 一般社団法人 日本土壌肥料学会, 2014年, 日本土壌肥料学会講演要旨集, 60 (0), 18 - 18, 日本語2-1-9 鉛直方向にともなう土壌有機物の質的・量的差異 : 1.水溶性有機物の隣接層位間差に着目して(2-1 土壌有機・無機成分の構造・機能・ダイナミクス,2014年度東京大会)
- 一般社団法人日本土壌肥料学会, 2013年09月11日, 日本土壌肥料学会講演要旨集, (59), 23 - 23, 日本語2-1-15 化学構造特性、黒色度、炭素・窒素安定同位体比から黒色腐植酸の生成過程を考える : 腐植酸の細分画法からみえること(2-1 土壌有機・無機化学,2013年度名古屋大会)
- 一般社団法人日本土壌肥料学会, 2013年09月11日, 日本土壌肥料学会講演要旨集, 59 (59), 21 - 21, 日本語
京都議定書における温室効果ガス削減目標が設定されて以来，エネルギー消費への関心が広がるとともに炭素動態に関するデータが蓄積されてきた。我が国で削減目標('90年比6%減)に対する約束期間(2008～2012)内の達成見込みが得られた(2012年12月現在)背景には詳細なデータの積み上げがあった。その膨大なデータには各土地利用における土壌炭素変化量の算出も大きく貢献している。土壌炭素の変化量を算出するためには土壌炭素賦存量が必要であり，そのデータベースの構築と算出法の開発は大きな成果といえる。この成果は，さらに続く排出削減目標に対し，算出法の最適化を図るための基盤になると考えられる。そこで，本シンポジウムでは，国土面積の占有割合が高い森林と農地での土壌炭素賦存量と変化量の算出結果と今後の課題を取り上げた。一方，それら土地利用に含まれない領域として都市域の土壌炭素賦存量算出の問題点も指摘した。さらに，土壌炭素変化量に影響する土壌有機物の分解特性と存在形態に関する話題を挙げた。また，様々な土壌における有機物の多様性を質的データで示し，さらにその変化の方向性についても論及した。土壌炭素に関する量的・質的な取扱いの両面から日本の土壌炭素賦存量について論議を行った。
日本土壌肥料學會, 2013年06月, 日本土壌肥料学会誌, 84 (3), 224 - 229, 日本語[査読有り][招待有り]
記事・総説・解説・論説等（学術雑誌）
- 2013年, 日本陸水学会大会講演要旨集, 78th活性アルミナを用いた琵琶湖溶存有機物の分画法の検討
- 2013年, 日本分析化学会年会講演要旨集, 62nd琵琶湖水中のフミン物質の定量モニタリング
- 一般社団法人日本土壌肥料学会, 2012年09月04日, 日本土壌肥料学会講演要旨集, 58 (58), 27 - 27, 日本語
- 一般社団法人日本土壌肥料学会, 2012年09月04日, 日本土壌肥料学会講演要旨集, (58), 28 - 28, 日本語P2-10 ^1H NMR法によるDOM特性解析における前処理法の検討(2.土壌有機・無機化学,2012年度鳥取大会)
- 一般社団法人日本土壌肥料学会, 2012年09月04日, 日本土壌肥料学会講演要旨集, (58), 214 - 214, 日本語III-4 土壌有機物の特性からみた炭素賦存量評価に向けた課題(III 日本の土壌炭素賦存量を量・質の両面から考える,シンポジウム,2012年度鳥取大会)
- 一般社団法人日本土壌肥料学会, 2012年09月04日, 日本土壌肥料学会講演要旨集, (58), 21 - 21, 日本語2-2 ^1H NMR法による土壌生態系のDOM特性の把握 : フィンランド・ラミー地域の事例(2.土壌有機・無機化学,2012年度鳥取大会)
- 黒ボク土における腐植の集積とメカニズム
典型的な黒ボク土は黒く厚いA層をもつ。この黒さは言うまでもなく腐植によるものであり,黒ボク土が広く分布する我が国ではひときわこの黒い腐植についての関心が高く,化学構造特性や生成過程(腐植化過程)に関する多くの研究がおこなわれてきた。近年では陸域生態系における炭素貯留庫としての土壌の役割が認知されるようになりチェルノーゼムと双璧をなす黒ボク土の腐植集積メカニズムに対してより多くの関心が寄せられている。そこで本講演では黒く多量の炭素を蓄積している黒ボク土の腐植層は他の土壌の腐植と何が違うのか,どのようにしてそれらが生成されるのか,なぜ長期にわたって蓄積するのか,という話題にそって現況を概説する。なお,黒ボク土の土壌腐植の性質をもっとも反映するのは腐植酸であるため,主に腐植酸に焦点を絞って話を進める。
ペドロジスト編集部, 2011年12月, ペドロジスト, 55 (2), 93 - 98, 日本語 - 2011年12月, Pedologist, 55 (2), 99 - 102, 日本語シンポジウム −黒ボク土における腐植の集積とメカニズム
[招待有り]
記事・総説・解説・論説等（学術雑誌）
- 一般社団法人日本土壌肥料学会, 2011年08月08日, 日本土壌肥料学会講演要旨集, 57 (57), 26 - 26, 日本語
- 一般社団法人日本土壌肥料学会, 2011年08月08日, 日本土壌肥料学会講演要旨集, (57), 335 - 335, 日本語28 C_<18>シリカゲルおよびDAX-8による溶存有機物の逐次分画法の検討(関西支部講演会,2010年度各支部会講演要旨)
- 一般社団法人日本土壌肥料学会, 2010年09月07日, 日本土壌肥料学会講演要旨集, (56), 172 - 172, 英語P22-6 Combined application of plant growth regulators and metal chelators for the improvement of heavy metal phytoextraction
- 一般社団法人日本土壌肥料学会, 2010年09月07日, 日本土壌肥料学会講演要旨集, (56), 79 - 79, 日本語11-13 ツバキ科などを含む樹木根(圏)が土壌アルミニウムの形態に及ぼす影響(11.植物の有害元素,2010年度北海道大会)
- 2010年03月15日, 日本生態学会大会講演要旨集, 57th, 512, 日本語二次遷移の進行が黒ボク土腐植物質の諸特性に及ぼす影響
- 一般社団法人日本土壌肥料学会, 2009年09月15日, 日本土壌肥料学会講演要旨集, (55), 19 - 19, 日本語2-1 チェルノーゼム土壌における植物炭化物の分布と腐植組成との関係(2.土壌有機・無機化学,2009年度京都大会)
- 一般社団法人日本土壌肥料学会, 2009年09月15日, 日本土壌肥料学会講演要旨集, (55), 26 - 26, 日本語2-23 腐植物質がチンゲンサイの硝酸イオン吸収に与える影響(2.土壌有機・無機化学,2009年度京都大会)
- 一般社団法人日本土壌肥料学会, 2009年09月15日, 日本土壌肥料学会講演要旨集, (55), 26 - 26, 日本語2-22 植物のケイ酸吸収能力の差が新鮮火山灰のアルミニウム富化に及ぼす影響について(2.土壌有機・無機化学,2009年度京都大会)
- 2009年09月, 日本土壌肥料学会講演要旨集, 日本語ポドゾルの溶脱層および集積層から抽出される可動性腐植物質の特徴
研究発表ペーパー・要旨（全国大会，その他学術会議）
- AMER CHEMICAL SOC, 2009年08月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 238, 英語ENVR 131-Chemical properties of humic substances and sorption characteristics for pharmaceuticals
研究発表ペーパー・要旨（国際会議）
- 一般社団法人日本土壌肥料学会, 2008年09月09日, 日本土壌肥料学会講演要旨集, (54), 23 - 23, 日本語2-6 黒ボク土における植物炭化物の性状(2.土壌有機・無機化学,2008年度愛知大会)
- 一般社団法人日本土壌肥料学会, 2008年09月09日, 日本土壌肥料学会講演要旨集, (54), 22 - 22, 日本語2-2 ^<13>C NMRによる腐植物質の炭素官能基組成 : 反転ゲートデカップリング法とCPMAS法の比較(2.土壌有機・無機化学,2008年度愛知大会)
- 一般社団法人日本土壌肥料学会, 2008年09月09日, 日本土壌肥料学会講演要旨集, (54), 23 - 23, 日本語2-4 各種土壌フルボ酸の相違性に関する研究(2.土壌有機・無機化学,2008年度愛知大会)
- 2007年10月, Humic Substances Research, 3, 1 - 9, 日本語腐植物質研究の基礎講座 その１．腐植物質試料を得るために〜IHSS法
[査読有り]
記事・総説・解説・論説等（学術雑誌）
- 一般社団法人日本土壌肥料学会, 2007年08月22日, 日本土壌肥料学会講演要旨集, (53), 24 - 24, 日本語P2-6 オオムギの硝酸吸収に及ぼす腐植物質の影響(2.土壌有機・無機化学,2007年度東京大会)
- 一般社団法人日本土壌肥料学会, 2007年08月22日, 日本土壌肥料学会講演要旨集, (53), 18 - 18, 日本語2-2 炭素および窒素安定同位体比からみた腐植物質の時空間変動(2.土壌有機・無機化学,2007年度東京大会)
- 一般社団法人日本土壌肥料学会, 2007年08月22日, 日本土壌肥料学会講演要旨集, (53), 18 - 18, 日本語2-3 HPSECで細分画した黒ボク土腐植酸の^<13>C NMR特性(2.土壌有機・無機化学,2007年度東京大会)
- 一般社団法人 日本土壌肥料学会, 2007年, 日本土壌肥料学会講演要旨集, 53 (0), 18 - 18, 日本語
- 一般社団法人 日本土壌肥料学会, 2007年, 日本土壌肥料学会講演要旨集, 53 (0), 23 - 23, 日本語
- 一般社団法人日本土壌肥料学会, 2006年09月05日, 日本土壌肥料学会講演要旨集, (52), 81 - 81, 日本語P9-22 オオムギの硝酸吸収に及ぼす腐植物質の影響(9. 植物の無機栄養, 2006年度秋田大会講演要旨)
- 一般社団法人日本土壌肥料学会, 2006年09月05日, 日本土壌肥料学会講演要旨集, (52), 16 - 16, 日本語2-1 黒ボク土および褐色森林土の土壌断面における緑色成分の垂直分布(2. 土壌有機・無機化学, 2006年度秋田大会講演要旨)
The vertical distribution of the content of the chloroform-extractable green fraction (CEGF) in the soil profiles of four Japanese Andosols, one Japanese Cambisol and one Nepalese Cambisol was examined using a colorimetric method with an ultraviolet and visible spectrophotometer. In addition, the relationship between the CEGF content and the soil chemical properties was examined by statistical analysis. CEGF was detected in all the soil samples, and the content ranged from 0.02 to 1.16 g kg(-1). The CEGF content in the surface A horizon of the Andosol tended to be higher than in the Cambisol. The vertical distribution of the CEGF content in three soil profiles (two Andosol profiles and one Nepalese Cambisol) revealed maximum values in the subsurface horizon. In the Japanese Cambisol, the content of the CEGF was highest in the surface horizon. In the remaining two Andosol profiles containing a buried humus horizon, the content of the CEGF was highest in the buried humus horizon and the distribution was considerably different from that of organic carbon. Statistical analysis showed that the content of the CEGF displayed a significant positive correlation (P < 0.001, n = 36) with the contents of dithionite-citrate-bicarbonate-extractable Al (Ald), and with oxalate-oxalic acid-extractable Fe and Al (Feo and Alo, respectively). Furthermore, the content of the CEGF showed a significant positive correlation with the content of Alo (r = 0.944, P < 0.01, n = 6) in the soil with the highest content of CEGF in the profile. It was, therefore, suggested that CEGF is actively produced in humus-rich soils like Andosols, and that Ald, Feo and, in particular, Alo were associated with the vertical distribution of CEGF in the soil profiles.
BLACKWELL PUBLISHING, 2006年06月, SOIL SCIENCE AND PLANT NUTRITION, 52 (3), 274 - 283, 英語- 2006年03月10日, 日本地理学会発表要旨集 = Proceedings of the General Meeting of the Association of Japanese Geographers, (69), 116 - 116, 日本語ドイツ・ハルツ山地 Elend 近郊における微地形・植生と表層土壌の関係 : 土壌菌核粒子の分布特性に着目して
- 神戸大学, 2006年03月, 神戸大学大学院自然科学研究科紀要. A, 24, 11 - 22, 英語Properties of Humic Acid Like Substances (HALS) created from sugar and amino acid with volcanic ash by thermal incubation
- 一般社団法人 日本土壌肥料学会, 2006年, 日本土壌肥料学会講演要旨集, 52 (0), 20 - 20, 日本語
- 一般社団法人日本土壌肥料学会, 2005年09月06日, 日本土壌肥料学会講演要旨集, (51), 19 - 19, 日本語2-6 チェルノーゼム土壌における植物炭化物の分布(2.土壌有機・無機化学,日本土壌肥料学会 2005年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2005年09月06日, 日本土壌肥料学会講演要旨集, (51), 26 - 26, 日本語P2-7 土壌菌核粒子の形成と遊離鉄(ポスター紹介,2.土壌有機・無機化学,日本土壌肥料学会 2005年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2005年09月06日, 日本土壌肥料学会講演要旨集, (51), 26 - 26, 日本語P2-6 鉄型土壌菌核粒子について(ポスター紹介,2.土壌有機・無機化学,日本土壌肥料学会 2005年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2005年09月06日, 日本土壌肥料学会講演要旨集, (51), 20 - 20, 日本語2-7 HPLC法による土壌中の緑色成分分離定量法の開発(2.土壌有機・無機化学,日本土壌肥料学会 2005年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2005年09月06日, 日本土壌肥料学会講演要旨集, (51), 162 - 162, 日本語P20-6 石炭灰を投入したイナワラおよび木質系堆肥の堆肥化時における化学成分の経時的変化とその堆肥特性(ポスター紹介,20.土壌改良資材,日本土壌肥料学会 2005年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2005年09月06日, 日本土壌肥料学会講演要旨集, (51), 114 - 114, 日本語14-1 カザフスタン北部における土地利用が腐植酸の形態と組成に及ぼす影響(14.土地分類利用・景域評価,日本土壌肥料学会 2005年度大会講演要旨集)
- 日本微生物生態学会, 2005年, 日本微生物生態学会講演要旨集, (21), 97 - 97, 日本語PA-51 外生菌根菌Cenococcum geophilumの生化学的特徴(生理/増殖,ポスターセッションA,ポスター発表)
- 一般社団法人 日本土壌肥料学会, 2005年, 日本土壌肥料学会講演要旨集, 51 (0), 18 - 18, 日本語
- 一般社団法人日本土壌肥料学会, 2004年09月14日, 日本土壌肥料学会講演要旨集, (50), 289 - 289, 日本語森林土壌中の菌核様粒子から分離された糸状菌と細菌の分類と性状(関東支部講演会, 日本土壌肥料学会支部講演会講演要旨集2004年度)
- 一般社団法人日本土壌肥料学会, 2004年09月14日, 日本土壌肥料学会講演要旨集, (50), 163 - 163, 日本語石炭灰の緑化用土としての特性と緑化樹木に与える影響(21. 緑化技術, 2004年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2004年09月14日, 日本土壌肥料学会講演要旨集, (50), 159 - 159, 日本語A型腐植酸様物質を含む土壌改良資材の創出 (第三報) : 木質炭素源と石炭灰を用いたA型腐植酸様物質の創出とその分解特性(20. 土壌改良資材, 2004年度大会講演要旨集)
- 一般社団法人日本土壌肥料学会, 2004年09月14日, 日本土壌肥料学会講演要旨集, (50), 121 - 121, 日本語各国から採取したチェルノーゼムの理化学性と腐植の形態分析(14. 土地分類利用・景域評価, 2004年度大会講演要旨集)
- 日本水環境学会, 2004年02月10日, 水環境学会誌 = Journal of Japan Society on Water Environment, 27 (2), 86 - 91, 日本語腐植物質の分析手法と構造特性の解析
- 一般社団法人 日本土壌肥料学会, 2004年, 日本土壌肥料学会講演要旨集, 50 (0), 20 - 20, 日本語
- 2004年, 日本土壌肥料学会誌, 75,4,511-517, 日本語陸域生体環境での土壌有機成分の役割とその機能
その他
- PERGAMON-ELSEVIER SCIENCE LTD, 2003年09月, GEOCHIMICA ET COSMOCHIMICA ACTA, 67 (18), A558 - A558, 英語Reactivity of enzymes to humic substances
研究発表ペーパー・要旨（国際会議）
- PERGAMON-ELSEVIER SCIENCE LTD, 2003年09月, GEOCHIMICA ET COSMOCHIMICA ACTA, 67 (18), A112 - A112, 英語Relation between biostability and chemical properties of soil humic substances
研究発表ペーパー・要旨（国際会議）
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 14 - 14, 日本語2-2 フルボ酸メチル誘導体の作成とそのHPSEC分析について(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 16 - 16, 英語2-7 Effect of Phosphate and pH on humification of Three Plant Residues with Fresh Volcanic Ashes
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 16 - 16, 日本語2-8 A型腐植酸様物質を含む土壌改良資材の創出(第三報) : 創出したA型腐植酸様物質の化学構造特性(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 167 - 167, 日本語22-13 神戸市周辺のため池に存在する水中有機物の特性および類型化(22.環境保全)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 134 - 134, 日本語17-2 和歌山県田辺市周辺のウメ園における土壌マンガン含量について(17.園地・施設土壌肥よく度)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 24 - 24, 日本語2-32 土壌菌核様粒子の化学組成および化学結合状態について(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 37 - 37, 日本語6-6 妙高燕土壌中の菌核粒子から分離されたSphingomonasの分類と芳香族化合物分解特性(6.土壌生物)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 24 - 24, 日本語2-33 土壌菌核粒子内にみられる生体鉱物について(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2003年08月20日, 日本土壌肥料学会講演要旨集, (49), 221 - 221, 日本語VI-5 腐植構造特性のバリエーションと環境・生態系との関わり(VI 陸域生態環境での土壌有機物成分の役割とその機能)
- 日本雑草学会, 2002年04月13日, 雑草研究. 別号, 講演会講演要旨, (41), 216 - 217, 日本語P24 土壌の違いによるアレロケミクスの作用発現の差異
- 一般社団法人日本土壌肥料学会, 2002年03月25日, 日本土壌肥料学会講演要旨集, (48), 232 - 232, 日本語1 湖沼堆積物の環境科学的研究(第一報)(関西支部講演会)
- 一般社団法人日本土壌肥料学会, 2002年03月25日, 日本土壌肥料学会講演要旨集, (48), 86 - 86, 英語13-7 Morphological, physical and chemical characterization of some Tanzanian volcanic ash soils
- 一般社団法人日本土壌肥料学会, 2002年03月25日, 日本土壌肥料学会講演要旨集, (48), 87 - 87, 日本語13-10 テフラに存在する腐植酸Pgの特性と成因について(13.土壌生成・分類)
- 一般社団法人日本土壌肥料学会, 2002年03月25日, 日本土壌肥料学会講演要旨集, (48), 9 - 9, 英語2-13 Effect of Different Forms of Inorganic Materials on Humification of Various Plant Residues
- 一般社団法人日本土壌肥料学会, 2002年03月25日, 日本土壌肥料学会講演要旨集, (48), 9 - 9, 英語2-14 Comparison of Inital Stage of Humic Acid Formation from Various Plant residues and nonhumic model substances.
- 一般社団法人日本土壌肥料学会, 2002年03月25日, 日本土壌肥料学会講演要旨集, (48), 7 - 7, 日本語2-7 各種腐植物質の^<13>C NMR特性(2.土壌有機・無機化学)
- 養賢堂, 2002年03月, 農業および園芸, 77 (3), 403 - 411, 日本語現代土壌肥料学の断面(15)腐植物質の化学構造をどうイメージするか
- Northeastern University, 2002年, Proceedings of International Humic Substances Society Twentieth Anniversary Conference, Humic substances : Nature's Most Versatile Materials, 56 - 57Molecular weight distribution of methylated humic acids in organic solvent system
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 191 - 191, 日本語21-15 各種副資材を投入した伐採木チップ堆肥の特性(21.土壌改良資材)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 203 - 203, 日本語23-16 神戸市周辺のため池に存在する水中懸濁物質(SS)の有機物特性(23.地域環境)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 127 - 127, 日本語13-16 土壌から検出される菌核様粒子の形態と化学組成の比較(13.土壌生成・分類)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 128 - 128, 日本語13-17 ドイツ・ハーツ山における菌核様粒子の分布と土壌の性状(13.土壌生成・分類)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 13 - 13, 日本語2-14 異なる腐植酸中に含まれる緑色成分(Pg)の比較(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 15 - 15, 日本語2-17 各種酵素の腐植酸分解活性の検討(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 14 - 14, 日本語2-16 A型腐植酸を含む土壌改良資材の創出(第二報) : 木質炭素源(チップ材)と石炭灰を用いたA型腐植酸の創出(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 13 - 13, 日本語2-13 pH別逐次抽出法で得られた腐植酸の特性 : ^<13>C NMRとSECについて(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2001年03月25日, 日本土壌肥料学会講演要旨集, (47), 14 - 14, 日本語2-15 メチル化処理の利用による腐植酸の研究 : IV平均分子量と分子量分布について(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 117 - 117, 日本語13-3 六甲山に分布する火山灰土の存在(13.土壌生成・分類)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 17 - 17, 日本語2-23 糸状菌による腐植酸の褐色に及ぼす粘土の影響(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 13 - 13, 日本語2-15 各種植物遺体と新鮮火山灰の反応に基づく腐植酸のスペクトル特性(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 13 - 13, 日本語2-16 A型腐植酸を含む土壌改良資材の創出(第一報) : イナワラから石炭灰を用いたA型腐植酸の創出(2.土壌有機・無機化学)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 325 - 325, 日本語10 湿原および池沼水中の腐植物質の特徴付け(関西支部講演会)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 35 - 35, 日本語6-6 妙高燕土壌中の菌核粒子微生物フロラの解析(6.土壌生物)
- 一般社団法人日本土壌肥料学会, 2000年03月25日, 日本土壌肥料学会講演要旨集, (46), 35 - 35, 日本語6-5 妙高燕土壌断面における菌核の分布と化学組成(6.土壌生物)
- Occurrence of Smectite on the Pumice Surface Derived from Taal Volcano in the Phillppines
The pumice layer was derived from the northwest fringe of Taal Volcano somma in Kaybagal, Tagaytay City in the Philippines. It has a gravel diameter of 3 to 5 centimeters (cm). The pumice gravel has a yellow surface color and a black inner color. These were observed using an optical microscope and scanning electron microscope (SEM). The chemical and mineralogical constituents were determined using a electron probe micro analyzer (EPMA), and X-ray diffraction apparatus. The results obtained from the optical microscope and SEM showed bubbles in the black or inner part of the pumice, while the surface showed weathered fine particles. Some of the bubbles observed were also partly weathered. The X-ray diffraction pattern showed smectite as the predominant mineral in the yellow surface layer, while amorphous materials were predominate in the black inner part. We speculate that smectite of the pumice may result from the weathering of amorphous glass in the inner part of pumice.
日本ペドロジー学会, 1999年06月30日, ペドロジスト, 43 (1), 22 - 27, 英語 - エコロジー緑化工法造成林における植生と土壌の経年変化 第2報 土壌の経年変化
り造成された,植栽後1年(ZR),11年(TN),18年(TW)経過した造成林と対照としての自然林(NF)において,植生の経年変化,土壌の経年変化,および植生と土壌の関係を調べた。一連の調査により次のことを明らかにした。1)造成林の3地点の土壌は礫の多いSLまたはLSの土性で,花崗岩質の真砂土の母材の特性を反映したものと推察した。また,O層およびA層の厚さ,土色の黒味,根量および緻密度はいずれも経年的に増加していた。2)経年数の長いTWより短いTNの方が容積重は少なく,孔隙量は多い層もあったが,容積重および孔隙量については経年的にそれぞれ減少,増加する傾向が認められ,植物根と土壌構造が発達したためと考察した。3)pHは造成当初の弱アルカリ性から経年的に酸性側に低下した。造成林ではCaとMgが自然林より著しく多く,陽イオン飽和度は100%以上で,pHは中〜微アルカリ性を示した。これは,造成時に混入された肥料由来のCaとMgが集積しているためと推察した。4)全炭素含量,全窒素含量および陽イオン交換容量は年数と共に増加する傾向を示し,この特徴は吸収根分布層(0〜30cm深)土壌でより明瞭に認められた。5)微生物バイオマスCは年数と共に増加した。栄養源の全炭素含量の増加が関係していると推察した。6)植生と土壌との関係については以下のように考察した。すなわち,造成林では年数と共に植生の階層構造の発達,D^2Hのような植物体の現存量の増加,侵入種による種数の増加が認められ,植物体の現存量の増加に伴って植物遺体のリターの供給が増加し,これらが最終的に土壌中の全炭素含量と全窒素含量を増加させた。このような土壌の全炭素量を増加させるような要因によって陽イオン交換容量と微生物バイオマスCが増加した。7)TWよりTNの方が植物体の現存量が大きかったのは,TNのほうがTWより孔隙量などに由来する水分や空気の保持力が大きかったためと推察した。しかし,そのTNの下層は硬度が大きく,水分や空気の保持力も小さいことが明らかにされ,今後は植物根の伸長がかなり制限されることを予測した。
日本ペドロジー学会, 1998年06月30日, ペドロジスト, 42 (1), 33 - 43, 日本語 - エコロジー緑化工法造成林における植生と土壌の経年変化 第1報 植生の経年変化
潜在自然植生の照葉樹の幼樹を密植するエコロジー緑化工法により姫路市南部の臨海工業地帯の緑地内に造成された,造成後の経過年数1年,11年および18年の造成林3地点(ZR, TNおよびTW)と対照の照葉樹の自然林1地点(NF)において植生調査を行い,それらを経時的な変化とみなして植生の遷移を考察した。結果を要約すると次のとおりである。1)造成林の群落高はZRの1m前後からTNおよびTWでは10m前後に増加したが,造成後の経過年数の大きいTWのほうが小さいTNよりも低かった。また,階層構造もZRの1層からTNおよびTWの3層へと分化し,高木層の優占種はTNではクスノキ,クロガネモチおよびカクレミノ,TWではマテバシイ,タブノキおよびヤマモモであった。一方,自然林のNFの群落高および階層構造は造成林よりも大きい20m前後および5層で,高木層の優占種はコジイ,アラカシおよびアカシデであった。2)造成林の単位面積あたりの幹数はNFに比べて2〜4倍程度多かった。TNおよびTWの胸高断面積(BA)合計は,ZRに比べて20〜40倍に増加したが,NFに比べて単位面積あたりでは概ね同様であったものの,幹1本あたりでは30%前後しかなかった。また,D^2HもTNおよびTWではZRよりも200〜300倍に増加していたが,NFに比べて単位面積あたりでは40%前後,幹1本あたりでは10%前後しかなかった。3)幹数およびD^2Hの優占種の数はZR, TNおよびNFでは2,3種,TWでは5,6種であったが,それらの組成は異なっていた。4)樹高階級の頻度分布はZRでは2m以下が100%を占めていた。TNおよびTWでは最大値が4〜6mであったが,TWでは二つ目の極大値が2m以下にも認められた。一方,NFでは1.3〜2mの30%から階級が高くなるにつれて漸減した。クスノキの幼樹はTNの6m以下の階級には認められなかったが,TWでは優占する6種のすべてに幼樹が認められた。また,直径階級分布も樹高階級分布と概ね同様の分布様式であった。TNおよびTWのいずれも胸高直径と樹高との間には正の相関関係が認められた。しかし,それらのプロットの分布様式は異なり,TNは5m前後で傾きが緩やかになり,TWは3m前後で分布が切れる特徴が認められた。5)造成林は潜在自然植生の自然林に発達する途中の段階にあり,今後は自然淘汰による幹数の減少にともなって階層構造およびD^2H等のバイオマスを増加させて自然林と同様の植生に発達すること,しかし,自然淘汰が起こらない場合には衰退の可能性もあること,これらのバイオマスの発達には土壌の性質が深く関係することを考察した。
日本ペドロジー学会, 1998年06月30日, ペドロジスト, 42 (1), 21 - 32, 日本語 - JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1995年09月, SOIL SCIENCE AND PLANT NUTRITION, 41 (3), 624 - 624, 英語THE SHAPE OF HUMIC-ACID IN SOLUTION AS OBSERVED BY SMALL-ANGLE X-RAY-SCATTERING (VOL 41, PG 363, 1995)
その他
- THE SHAPE OF HUMIC-ACID IN SOLUTION AS OBSERVED BY SMALL-ANGLE X-RAY-SCATTERING
Electromagnetic scattering (e.g., light scattering, small-angle X-ray scattering, and small-angle neutron scattering) provides information on the size and shape of colloidal particles in a highly dispersed system (Feigin and Svergun 1987). The technique has been applied to various materials including biological macromolecules, synthetic polymers, amorphous solids, and other colloidal compounds (Feigin and Svergun 1987). Several attempts have also been made to evaluate the size and shape of humic acid in solution using the scattering techniques (Wershaw and Pinckney 1989; Osterberg et al. 1993). Small-angle X-ray scattering (SAXS) was first applied to sodium humate by Wershaw et al. (1967) and they estimated the molecular weight, molecular volume, radius of gyration (R(g)), and shape factor of the sodium humate. Thereafter, based on studies of humic substances by SAXS, R(g) which can be estimated independently from the SAXS data of humic substances was obtained as the sole parameter because of the lack of information on the chemical structure of humic substances (Lindqvist 1970; Wershaw and Pinckney 1971, 1973, 1977; Thurman et al. 1982). In principle, however, it is possible to evaluate the shape of particles by analyzing the profile of the SAXS data, because the scattered intensity and profile reflect the number and spatial distribution of the scattering units composing colloidal particles. The excess scattered intensity is Fourier-transformed into the distance distribution function which represents the electron density distribution in a particle (Kajiwara et al. 1991). The shape of humic acid can be deduced approximately from the distance distribution function without any chemical information. In addition to the distance distribution function, various methods can be used to analyze the shape of objects from the scattering profile.; Fer example, the Kratky plot yields information on the chain stiffness of polymers or in some cases on the architecture of a molecule (Donkai et al. 1985). The purpose of this study is to analyze the size and shape of humic acid by using the SAXS data from the solution.
JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1995年06月, SOIL SCIENCE AND PLANT NUTRITION, 41 (2), 363 - 366, 英語記事・総説・解説・論説等（学術雑誌）
- JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1995年03月, SOIL SCIENCE AND PLANT NUTRITION, 41 (1), 147 - 150, 英語PREPARATION OF HUMIC-ACID FRACTIONS WITH A DEFINITE RANGE OF PARTICLE SIZES BY GEL-PERMEATION CHROMATOGRAPHY (GPC)
記事・総説・解説・論説等（学術雑誌）
- 日本ペドロジー学会, 1993年06月30日, ペドロジスト, 37 (1), 59 - 60, 日本語「第二回ペドロジストトレーニングコース」を終えて(プロファイル)
- JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1992年09月, SOIL SCIENCE AND PLANT NUTRITION, 38 (3), 579 - 583, 英語VERTICAL-DISTRIBUTION OF CHRYSOTALUNIN, A MAJOR SOIL ANTHRAQUINONE PIGMENT, IN SOIL PROFILES
記事・総説・解説・論説等（学術雑誌）
- JAPANESE SOC SOIL SCIENCE PLANT NUTRITION, 1991年06月, SOIL SCIENCE AND PLANT NUTRITION, 37 (2), 363 - 366, 英語OCCURRENCE OF 7,7'-BIPHYSCION IN JAPANESE SOILS
記事・総説・解説・論説等（学術雑誌）
書籍等出版物
- 共著, 農文協, 2019年04月, 日本語, ISBN: 9784540181870腐植物質分析ハンドブックー標準試料を例にして 第2版
学術書
- その他, 文英堂出版, 2018年02月, 日本語土壌サイエンス入門 第２版 「第11章 地球を支える土壌の機能」
学術書
- その他, 朝倉書店, 2015年09月, 日本語土のひみつー食料・環境・生命 「2-4 土の生成に及ぼす気候(温度・水分状態) の影響」
学術書
- その他, 博友社, 2011年03月, 日本語, ISBN: 9784826802130土壌―河川―海生態系における溶存有機炭素の動態と機能
学術書
- その他, 三共出版, 2008年11月, 日本語, ISBN: 9784782705773環境中の腐植物質 : その特徴と研究法
学術書
- その他, 地人書館, 2008年10月, 日本語, ISBN: 9784805208052微生物増殖学の現在・未来
学術書
- その他, 三恵社, 2007年10月, 日本語, ISBN: 9784883615704腐植物質分析ハンドブック : 標準試料を例にして
学術書
- その他, Embarapa Instrumentacao Agropecuaria, 2004年12月, 英語, ISBN: 8586463124Humic substances and soil and water environment
学術書
- その他, Polish Society of Humic Substances, 1997年, 英語, ISBN: 8390640325The role of humic substances in the ecosystems and in environmental protection
学術書
講演・口頭発表等
- 日本土壌肥料学会京都大会, 2015年09月, 日本語, 日本土壌肥料学会, 京都大学, 国内会議生分解性レジンコンクリートの分解制御方法の検討
ポスター発表
- 日本土壌肥料学会京都大会, 2015年09月, 日本語, 日本土壌肥料学会, 京都大学, 国内会議各種資材の土壌施用による丹波黒の連作障害の軽減方法の検討
ポスター発表
- 日本学術会議公開シンポジウム「分野横断型農学の新展開に向けて－物理学・数理学の視点とその重要性－」, 2015年03月, 日本語, 日本学術会議農学委員会・食料科学委員会, 筑波大学東京キャンパス, 国内会議土・水環境に遍在するフミン物質の構造化学的特徴とその多様性
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 日本生態学会第62回全国大会, 2015年03月, 日本語, 鹿児島大学, 国内会議高緯度北極の湿原生態系における溶存有機物の特性
ポスター発表
- 日本腐植物質学会第30回講演会 記念シンポジウム, 2014年11月, 日本語, 日本大学, 国内会議水環境中の天然有機物の化学構造
シンポジウム・ワークショップパネル（指名）
- 日本腐植物質学会, 2014年11月, 日本語, 日本大学, 国内会議重金属を収着させた非晶質および結晶性粘土コロイドに対するフルボ酸の収脱着特性
口頭発表（一般）
- 日本腐植物質学会, 2014年11月, 日本語, 日本大学, 国内会議スコットランド地方の有色河川水におけるフミン物質濃度と溶存元素濃度の関係
ポスター発表
- 日本土壌肥料学会, 2014年09月, 日本語, 東京農工大学, 国内会議腐植酸資材及び無機成分施用が土壌ＣＥＣに及ぼす影響
口頭発表（一般）
- 日本陸水学会第79回大会（筑波大会）, 2014年09月, 日本語, 筑波大学, 国内会議活性アルミナを用いて分離した琵琶湖溶存有機物の化学的特徴
口頭発表（一般）
- 日本土壌肥料学会, 2014年09月, 日本語, 東京農工大学, 国内会議鉛直方向にともなう土壌有機物の質的・量的差異 1.水溶性有機物の隣接層位間差に着目して
口頭発表（一般）
- 日本土壌肥料学会, 2014年09月, 日本語, 東京農工大学, 国内会議ドイツBaden-Wurttemberg州の農耕地の腐植酸の化学構造特性に耕作が与える影響，日本土壌肥料学会東京大会
ポスター発表
- 17th Meeting of the International Humic Substances Society, 2014年09月, 英語, Ioannina, Greece, 国際会議The effect of Humic acid on the Acute Toxicity of Benzo[a]pyrene to Algae, Pseudokirchneriella subcapitata.
ポスター発表
- 17th Meeting of the International Humic Substances Society, 2014年09月, 英語, Ioannina, Greece, 国際会議Comparison of Structural Properties of Humic-Like Substances in Atmospheric Fine Aerosol Isolated by Different Methods.
口頭発表（一般）
- 日本土壌肥料学会, 2014年09月, 日本語, 東京農工大学, 国内会議C/N比の異なる堆肥の連用が土壌腐植酸の量的変化に及ぼす影響
口頭発表（一般）
- The 16th International Symposium on River and Lake Environments 2014, 2014年08月, 英語, Seoul, Korea, 国際会議Distribution and chemical characteristics of dissolved organic matter in Lake Biwa extracted by hydrophobic adsorbents.
ポスター発表
- The 16th International Symposium on River and Lake Environments 2014, 2014年08月, 英語, Seoul, Korea, 国際会議Distribution and chemical characteristics of dissolved organic matter in Lake Biwa extracted by activated alumina.
ポスター発表
- 京都工芸繊維大学環境科学センター第20回公開講演会「緑の地球と共に生きる」, 2014年06月, 日本語, 京都工芸繊維大学環境科学センター, 国内会議土からみた地球環境問題
公開講演，セミナー，チュートリアル，講習，講義等
- 日本生態学会第62回全国大会, 2014年03月, 日本語, 鹿児島大学, 国内会議スコットランド河川水中のフミン物質と溶存元素の関係
ポスター発表
- 日本陸水学会近畿支部会第25回研究発表会, 2014年02月, 日本語, 大阪, 国内会議国内外の湖沼や河川水のフミン物質含量〜DAX-8樹脂吸着法による〜
口頭発表（一般）
- 日本陸水学会近畿支部会第25回研究発表会, 2014年02月, 日本語, 大阪, 国内会議FT-ICR MSによる森林生態系内溶存有機物の分布と特徴の解析
口頭発表（一般）
- 8th Asian Aerosol Conference, Australia, 2013年12月, 日本語, Sydney, 国際会議Chemical structure of humic-like substances in fine urban aerosol
ポスター発表
- 日本腐植物質学会第29回講演会, 2013年11月, 日本語, 佐賀, 国内会議琵琶湖水中のフルボ酸の生物影響評価について
ポスター発表
- 日本腐植物質学会第29回講演会, 2013年11月, 日本語, 佐賀, 国内会議黒ボク土畑における堆肥施用が土壌腐植物質へ及ぼす影響
ポスター発表
- 日本腐植物質学会第29回講演会, 2013年11月, 日本語, 佐賀, 国内会議湖沼や河川水の腐植物質含量はどのくらいを占めるか
口頭発表（一般）
- 日本腐植物質学会第29回講演会, 2013年11月, 日本語, 佐賀, 国内会議ベンゾ[a]ピレンの生物毒性に対する腐植酸の影響 —緑藻に対する生育阻害軽減効果—
ポスター発表
- 日本腐植物質学会第29回講演会, 2013年11月, 日本語, 佐賀, 国内会議FT-ICR MSと3DEEMsを用いた森林生態系を循環する溶存有機物の動態の解析
ポスター発表
- 日本土壌肥料学会2013年度名古屋大会, 2013年09月, 日本語, 名古屋, 国内会議分子サイズ別画分の化学構造特性、黒色度、炭素・窒素安定同位体比から黒色腐植酸の生成過程を考える
口頭発表（一般）
- 日本土壌肥料学会2013年度名古屋大会, 2013年09月, 日本語, 名古屋, 国内会議腐植酸寒天培地を用いた有機汚染物質のバイオアッセイ
ポスター発表
- 日本陸水学会第78回大会, 2013年09月, 日本語, 大津, 国内会議琵琶湖水中のフルボ酸の生物影響評価手法について
ポスター発表
- 日本分析化学会第62年会, 2013年09月, 日本語, 大阪, 国内会議琵琶湖水中のフミン物質の定量モニタリング
ポスター発表
- 日本陸水学会第78回大会, 2013年09月, 日本語, 大津, 国内会議活性アルミナを用いた琵琶湖溶存有機物の分画法の検討
ポスター発表
- 日本地球惑星科学連合大会, 2013年05月, 日本語, 東京, 国内会議Characterization of dissolved organic matter using FT-ICR MS and fluorescence spectrometry in a Japanese Cypress forest ecosystem.
ポスター発表
- 陸水学会近畿支部大会, 2013年03月, 日本語, 奈良, 国内会議黒ボク土壌森林内における溶存有機物の動態に関する研究
ポスター発表
- 第47回日本水環境学会年会, 2013年03月, 日本語, 大阪, 国内会議湖沼に蓄積する難分解性溶存有機物の藻類に及ぼす影響
ポスター発表
- 陸水学会近畿支部大会, 2013年03月, 日本語, 奈良, 国内会議C18、DAX固相抽出法による琵琶湖溶存有機物の分離と特徴の比較
ポスター発表
- 日本腐植物質学会第28回講演会, 2012年11月, 日本語, 東京, 国内会議琵琶湖水中の水系フミン物質の季節および深度別の変化
口頭発表（一般）
- 日本腐植物質学会第28回講演会, 2012年11月, 日本語, 東京, 国内会議微生物による腐植酸の褪色に及ぼす粘土鉱物の影響－第2報－
口頭発表（一般）
- 日本腐植物質学会第28回講演会, 2012年11月, 日本語, 東京, 国内会議土壌腐植酸の化学構造特性に基づいた類型化
口頭発表（一般）
- 日本腐植物質学会第28回講演会, 2012年11月, 日本語, 東京, 国内会議大気中腐植様物質の水溶性・フルボ酸・腐植酸画分の化学構造特性
口頭発表（一般）
- 日本腐植物質学会第28回講演会, 2012年11月, 日本語, 東京, 国内会議各種腐植酸とそれらメチル化物に対する多環芳香族化合物の親和性
口頭発表（一般）
- 日本腐植物質学会第28回講演会シンポジウム, 2012年11月, 日本語, 東京, 国内会議フミン物質の化学構造特性とその多様性
[招待有り]
シンポジウム・ワークショップパネル（指名）
- シンポジウムIII「日本の炭素賦存量を量・質の両面から考える」，日本土壌肥料学会2012年度鳥取大会, 2012年09月, 日本語, 鳥取, 国内会議土壌有機物の特性からみた炭素賦存量評価に向けた課題
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 日本土壌肥料学会2012年度鳥取大会, 2012年09月, 日本語, 鳥取, 国内会議1H NMR法によるDOM特性解析における前処理法の検討
ポスター発表
- 日本土壌肥料学会2012年度鳥取大会, 2012年09月, 日本語, 鳥取, 国内会議1H-NMR 分析法による土壌生態系のDOM 特性の把握−フィンランド・ラミー地域の事例
口頭発表（一般）
- 第29回エアロゾル科学・技術研究討論会, 2012年08月, 日本語, 福岡, 国内会議TMAH熱化学メチル化GC/MS法による大気中腐植様物質の構造解析
ポスター発表
- The 22nd V. M. Goldschmit Conference, 2012年07月, 英語, Montreal Canada, 国際会議Structural characterization of humic-like substances in atmospheric aerosols in Osaka, Japan.
ポスター発表
- Acuatic Sciences Meeting of Association for the Sciences of Limnology and Oceanography 2012, 2012年07月, 英語, 大津, 国際会議Development and application of a method for quantitative analysis of aquatic humic substances in clear water
ポスター発表
- 日本陸水学会近畿支部会第23回研究発表会, 2012年03月, 日本語, 奈良, 国内会議琵琶湖水中の水系フミン物質の季節および深度別の変化
ポスター発表
- 日本ペドロジー学会2012年度大会, 2012年03月, 日本語, 東京, 国内会議C18シリカゲルおよびDAX-8樹脂による溶存有機物の分別定量法の開発
ポスター発表
- 日本腐植物質学会第26回講演会, 2011年11月, 日本語, 金沢, 国内会議微生物による腐植酸の褪色に及ぼす粘土鉱物の影響
ポスター発表
- 日本腐植物質学会第27回講演会, 2011年11月, 日本語, 金沢, 国内会議大気中の腐植様物質の濃度と化学構造特性
口頭発表（一般）
- 第50回公開シンポジウム 「黒ボク土の明と暗 ペドロジスとの提言」，日本ペドロジー学会2011年度大会, 2011年09月, 日本語, 日本ペドロジー学会, 帯広, 国内会議黒ボク土における腐植の集積とメカニズム
[招待有り]
シンポジウム・ワークショップパネル（指名）
- Finnish-Japanese Seminar of the Dynamics of Dissolved organic Matter in Forested Catchments under Human and Environmental Impacts, 2011年09月, 英語, Metla, Helsinki, Finland, 国際会議Quality analysis of dissolved organic matter by a sequential fractionation with C18 and DAX-8 resin
ポスター発表
- Finnish-Japanese Seminar of the Dynamics of Dissolved organic Matter in Forested Catchments under Human and Environmental Impacts, 2011年09月, 英語, Metla, Helsinki, Finland, 国際会議Characterization of dissolved organic matter in soils and streams using 1H-NMR spectra analysis
ポスター発表
- Finnish-Japanese Seminar of the Dynamics of Dissolved organic Matter in Forested Catchments under Human and Environmental Impacts, 2011年09月, 英語, Metla, Helsinki, Finland, 国際会議Characterization and grouping of humic substances in soils and waters by NMR spectra
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 日本土壌肥料学会2011年度つくば大会, 2011年08月, 日本語, 日本土壌肥料学会, 茨城, 国内会議糸状菌による腐植酸の褪色に及ぼす粘土の影響(第2報) : Na+型とCa2+型の比較
ポスター発表
- 第20回環境化学討論会, 2011年07月, 日本語, 日本環境化学会, 熊本, 国内会議医薬品類と水系フミン物質の吸着
ポスター発表
- 第20回環境化学討論会, 2011年07月, 日本語, 日本環境化学会, 熊本, 国内会議FRETペアとしての腐植物質と医薬品との相互作用‐スペクトル重なり積分と吸着係数の関係‐
ポスター発表
- 日本ペドロジー学会2011年度大会, 2011年03月, 日本語, 日本ペドロジー学会, 帯広, 国内会議高緯度地域における溶存有機物（DOM）の化学構造特性の把
ポスター発表
- 日本化学会関東支部茨城地区研究交流会, 2011年03月, 日本語, 国内会議ピレンの親和性に及ぼす腐植酸の構造特性の影響
口頭発表（一般）
- 日本ペドロジー学会2011年度大会, 2011年03月, 日本語, 日本ペドロジー学会, 帯広, 国内会議C18およびDAX-8による水抽出有機物疎水性画分の細分画法の検討
ポスター発表
- 日本腐植物質学会第26回講演会, 2010年11月, 日本語, 日本腐植物質学会, 筑波, 国内会議溶存フミン物質の分画法の開発
口頭発表（一般）
- 日本腐植物質学会第26回講演会, 2010年11月, 日本語, 日本腐植物質学会, 筑波, 国内会議大阪市の大気エアロゾルに含まれる腐植物質の特性
口頭発表（一般）
- 日本腐植物質学会第26回講演会, 2010年11月, 日本語, 日本腐植物質学会, 筑波, 国内会議ピレンの微生物分解に及ぼす腐植酸の影響
ポスター発表
- 日本土壌肥料学会2010年度北海道大会, 2010年09月, 日本語, 日本土壌肥料学会, 北海道, 国内会議ツバキ科などを含む樹木根(圏)が土壌アルミニウムの形態に及ぼす影響
口頭発表（一般）
- 日本土壌肥料学会2010年度北海道大会, 2010年09月, 日本語, 日本土壌肥料学会, 北海道, 国内会議Combined application of plant growth regulators and metal chelators for the improvement of heavy metal phytoextraction
ポスター発表
- 日本腐植物質学会第25回講演会, 2009年11月, 日本語, 日本腐植物質学会, 兵庫, 国内会議腐植物質に対する医薬品類の収着
口頭発表（一般）
- 日本腐植物質学会第25回講演会, 2009年11月, 日本語, 日本腐植物質学会, 兵庫, 国内会議Trametes versicolorラッカーゼのフルボ酸褪色研究への適用ー固定化酵素による逐次反応と反応後フルボ酸の分析ー
口頭発表（一般）
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議腐植物質がチンゲンサイの硝酸イオン吸収に与える影響
口頭発表（一般）
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議二次遷移の進行に伴い黒ボク土腐植物質の諸特性はどうなるのか? : 腐植酸の炭素および窒素安定同位体比
口頭発表（一般）
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議二次遷移の進行に伴い黒ボク土腐植物質の諸特性はどうなるのか? : 腐植酸とフルボ酸の化学構造特性解析
口頭発表（一般）
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議植物のケイ酸吸収能力の差が新鮮火山灰のアルミニウム富化に及ぼす影響について
口頭発表（一般）
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議固定化ラッカーゼによるフルボ酸の褪色
口頭発表（一般）
- 第74回日本陸水学会, 2009年09月, 日本語, 日本陸水学会, 国内会議固相抽出を用いた琵琶湖溶存有機物の化学分画に関する基礎的研究１
ポスター発表
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議ポドゾルの溶脱層および集積層から抽出される可動性腐植物質の特徴
口頭発表（一般）
- 日本土壌肥料学会2009年度京都大会, 2009年09月, 日本語, 日本土壌肥料学会, 京都, 国内会議チェルノーゼム土壌における植物炭化物の分布と腐植組成との関係
口頭発表（一般）
- The 238th American Chemical Society National Meeting, 2009年08月, 英語, American Chemical Society, Washington DC, USA, 国際会議Chemical properties of humic substances and sorption characteristics for pharmaceuticals
ポスター発表
- International Symposium on Soil Organic Matter Dynamics: Land Use, Management and Global Change, 2009年07月, 英語, Colorado Springs, USA, 国際会議The effects of ecological succession on the characteristic of soil humic substances in a Japanese volcanic ash soil. Concentration and chemical properties of humic substances
ポスター発表
- International Symposium on Soil Organic Matter Dynamics: Land Use, Management and Global Change, 2009年07月, 英語, Colorado Springs, USA, 国際会議The effects of ecological succession on the characteristic of soil humic substances in a Japanese volcanic ash soil: Chemical properties and stabilities of different size fractions of Humic acids
ポスター発表
- 第70回分析化学討論会, 2009年05月, 日本語, 日本分析化学会, 国内会議天然水中のフミン物質の定量法
ポスター発表
- 日本ペドロジー学会2008年度大会, 2009年03月, 日本語, 日本ペドロジー学会, 京都, 国内会議腐植酸の諸特性から見た黒ボク土と褐色森林土の関連性〜Ｉ 分取ＨＰＳＥＣ法による分子サイズ別分画と光学的特性〜
ポスター発表
- 日本ペドロジー学会2009年度大会, 2009年03月, 日本語, 日本ペドロジー学会, 京都, 国内会議腐植酸の諸特性から見た黒ボク土と褐色森林土の関連性〜メチル化法を用いた腐植酸の物理化学構造特性解析〜
ポスター発表
- 日本ペドロジー学会2008年度大会, 2009年03月, 日本語, 日本ペドロジー学会, 京都, 国内会議腐植酸の諸特性から見た黒ボク土と褐色森林土の関連性〜II 各分子サイズ別画分の元素組成と13Ｃ ＮＭＲ特性〜
ポスター発表
- 第48回シンポジウム「大陸中央部冷温帯下の土壌の分布，特性，生成・分類−ユーラシア・北米の草原から森林へ−」日本ペドロジー学会2009年度大会, 2009年03月, 日本語, 日本ペドロジー学会, 京都, 国内会議ユーラシアおよびカナダのステップにおけるチェルノーゼム土壌とその腐植物質の特性
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 第24回日本腐植物質学会講演会, 2008年11月, 日本語, 日本腐植物質学会, 北海道, 国内会議非有色水系の腐植物質定量法の検討—樹脂吸着（バッチ法）からのアプローチ
口頭発表（一般）
- 第24回日本腐植物質学会講演会, 2008年11月, 日本語, 日本腐植物質学会, 北海道, 国内会議土壌，堆積物および溶存腐植物質の水素同位体比
口頭発表（一般）
- 日本分析化学会近畿支部平成20年度第３回提案公募型セミナー「水圏の腐植物質研究会」, 2008年11月, 日本語, 日本分析化学会近畿支部, 神戸, 国内会議土壌と陸水の腐植物質の化学構造特性
公開講演，セミナー，チュートリアル，講習，講義等
- 第24回日本腐植物質学会講演会, 2008年11月, 日本語, 日本腐植物質学会, 北海道, 国内会議液体および固体13C NMR法による腐植物質の官能基炭素組成の比較 — 反転ゲートデカップリング法とCPMAS法
口頭発表（一般）
- 日本土壌肥料学会2008年度愛知大会, 2008年09月, 日本語, 日本土壌肥料学会, 愛知, 国内会議黒ボク土における植物炭化物の性状
口頭発表（一般）
- 日本土壌肥料学会2008年度愛知大会, 2008年09月, 日本語, 日本土壌肥料学会, 愛知, 国内会議各種土壌フルボ酸の相違性に関する研究
口頭発表（一般）
- 日本土壌肥料学会2008年度愛知大会, 2008年09月, 日本語, 日本土壌肥料学会, 愛知, 国内会議13C NMRによる腐植物質の炭素官能基組成 : 反転ゲートデカップリング法とCPMAS法の比較
口頭発表（一般）
- 第17回環境化学討論会, 2008年06月, 日本語, 環境化学会, 神戸, 国内会議腐植物質から見た環境研究
[招待有り]
口頭発表（招待・特別）
- 日本腐植物質学会第23回講演会, 2007年11月, 日本語, 日本腐植物質学, 弘前, 国内会議土壌および沢水中の水溶性腐植物質の化学特性〜黒ボク土壌地帯と褐色森林土壌地帯との比較
口頭発表（一般）
- 日本腐植物質学会第23回講演会, 2007年11月, 日本語, 日本腐植物質学, 弘前, 国内会議環境中の腐植物質の炭素および窒素安定同位体比の時空間変動
口頭発表（一般）
- 日本腐植物質学会第23回講演会, 2007年11月, 日本語, 日本腐植物質学, 弘前, 国内会議Trametes versicolorラッカーゼのフルボ酸褪色研究への適用〜Trametes villosaラッカーゼとの比較
口頭発表（一般）
- 日本腐植物質学会第23回講演会, 2007年11月, 日本語, 日本腐植物質学, 弘前, 国内会議HPSEC分画法による土壌腐植酸の特性解析: 黒ボク土腐植酸と褐色森林土腐植酸の関連性へのアプローチ
口頭発表（一般）
- 日本腐植物質学会第23回講演会, 2007年11月, 日本語, 日本腐植物質学, 弘前, 国内会議C18固相抽出により分離された琵琶湖溶存有機物の特徴〜IHSS法により抽出された溶存腐植物質との比較
口頭発表（一般）
- 日本腐植物質学会第23回講演会, 2007年11月, 日本語, 日本腐植物質学, 弘前, 国内会議AMS14C年代測定にもとづく森林土壌における土壌菌核粒子の滞留時間
口頭発表（一般）
- 第２回菅平高原実験センター研究集会, 2007年10月, 日本語, 筑波大学菅平高原実験センター, 長野, 国内会議土壌腐植の生成・分解プロセスと移行挙動に関する研究
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 日本陸水学会第72回大会, 2007年09月, 日本語, 日本陸水学会, 茨城, 国内会議河川溶存腐植物質の化学構造特性のバリエーション
口頭発表（一般）
- 日本土壌肥料学会2007年度東京大会, 2007年08月, 日本語, 日本土壌肥料学会, 東京, 国内会議日本各地における土壌溶存腐植物質の13C NMR特性
口頭発表（一般）
- 日本土壌肥料学会2007年度東京大会, 2007年08月, 日本語, 日本土壌肥料学会, 東京, 国内会議土壌および沢水中の水溶性腐植物質の化学構造特性と動態 : 黒ボク土壌地帯と褐色森林土壌地帯との比較
ポスター発表
- 日本土壌肥料学会2007年度東京大会, 2007年08月, 日本語, 日本土壌肥料学会, 東京, 国内会議炭素および窒素安定同位体比からみた腐植物質の時空間変動
口頭発表（一般）
- 日本土壌肥料学会2007年度東京大会, 2007年08月, 日本語, 日本土壌肥料学会, 東京, 国内会議異なるフルボ酸に対するLaccaseの褪色作用
口頭発表（一般）
- 日本土壌肥料学会2007年度東京大会, 2007年08月, 日本語, 日本土壌肥料学会, 東京, 国内会議オオムギの硝酸吸収に及ぼす腐植物質の影響
ポスター発表
- 日本土壌肥料学会2007年度東京大会, 2007年08月, 日本語, 日本土壌肥料学会, 東京, 国内会議HPSECで細分画した黒ボク土腐植酸の13C NMR特性
口頭発表（一般）
- 8th Conference of the East and Southeast Asian Federation of Soil Science, 2007年08月, 英語, Taiwan, 国際会議Chemical properties of humic substances of Andosoil in Japan
ポスター発表
- 3rd International Conference on Mechanisms of Organic Matter Stabilisation and Destabilisation in Soils and Sediments, 2007年07月, 英語, Adelaid, Austalia, 国際会議Comparisons of chemical properties of water-soluble humic substances in litter, mineral soil and stream
ポスター発表
- International Symposium on Organic Matter Dynamics in Agro-Ecosystems, 2007年07月, 英語, Poitiers, 国際会議Aluminum and iron contents of sclerotium grains and their role as persistent organic component in forest soils
ポスター発表
- 日本地理学会春季学術大会, 2007年05月, 日本語, 日本地理学会, 東京, 国内会議植生・標高系列における森林土壌中の菌核粒子分布の変化-青森県岩木山，岐阜県御嶽山を例として-
口頭発表（一般）
- 日本腐植学会, 2005年, 日本語, 日本腐植学会, 神戸, 国内会議腐植物質の多様性とインベントリー作成
口頭発表（一般）
- 日本腐植学会, 2005年, 日本語, 日本土壌肥料学会, 神戸, 国内会議土地利用形態の異なる黒ボク土における腐植酸褪色菌数の差異
口頭発表（一般）
- 土肥学会, 2005年, 日本語, 日本土壌肥料学会, 島根, 国内会議土壌菌核粒子の形成と遊離鉄
口頭発表（一般）
- 土肥学会, 2005年, 日本語, 日本土壌肥料学会, 島根, 国内会議土壌および周辺沢中の水溶性腐植物質の構造特性 季節による変動
口頭発表（一般）
- 日本腐植学会, 2005年, 日本語, 日本腐植学会, 神戸, 国内会議土壌および周辺沢水中の水溶性腐植物質におけるNMR特性の季節変化
口頭発表（一般）
- 土肥学会, 2005年, 日本語, 日本土壌肥料学会, 島根, 国内会議鉄型土壌菌核粒子について
口頭発表（一般）
- 土肥学会, 2005年, 日本語, 日本土壌肥料学会, 島根, 国内会議黒ボク土における腐植酸褪色菌数と土地利用形態との関係
口頭発表（一般）
- 土肥学会, 2005年, 日本語, 日本土壌肥料学会, 島根, 国内会議HPLC法による土壌中の緑色成分分離定量法の開発
口頭発表（一般）
- 日本土壌肥料学会, 2004年, 日本語, 日本土壌肥料学会, 福岡, 国内会議Ａ型腐植酸様物質を含む土壌改良資材の創出（第三報）木質炭素源と石炭灰を用いたＡ型腐植酸様物質の創出とその分解特性
口頭発表（一般）
- 日本腐植物質学会第20回講演会, 2004年, 日本語, 日本腐植物質学会, 千葉, 国内会議木質廃棄物とケイフンから作成した堆肥の腐熟化に伴う腐植酸様物質の化学構造特性の変化
口頭発表（一般）
- 日本土壌肥料学会, 2004年, 日本語, 日本土壌肥料学会, 福岡, 国内会議土壌中の腐植酸褪色菌の菌数ならびに褪色活性の測定
口頭発表（一般）
- 日本土壌肥料学会, 2004年, 日本語, 日本土壌肥料学会, 福岡, 国内会議土壌からHCI-DMSOで抽出される緑色色素について
口頭発表（一般）
- 日本土壌肥料学会, 2004年, 日本語, 日本土壌肥料学会, 福岡, 国内会議石炭灰の緑化用土としての特性と緑化樹木に与える影響
口頭発表（一般）
- 日本腐植物質学会第20回講演会, 2004年, 日本語, 日本腐植物質学会, 千葉, 国内会議水溶性腐植物質の動態と構造特性
口頭発表（一般）
- 日本土壌肥料学会, 2004年, 日本語, 日本土壌肥料学会, 福岡, 国内会議リター及び土壌中の水溶性腐植物質の採取法についての検討
ポスター発表
- 日本腐植物質学会第20回講演会, 2004年, 日本語, 日本腐植物質学会, 千葉, 国内会議フルボ酸退色研究における固定化Laccaseの利用
口頭発表（一般）
- 日本腐植物質学会第１９回講演会講演要旨集, 2003年11月, 日本語, 日本腐植物質学会, 佐賀大学, 国内会議分取HPSEC で得られた腐植酸の粒子サイズ別画分の構造特性
口頭発表（一般）
- 日本腐植物質学会第１９回講演会講演要旨集, 2003年11月, 日本語, 日本腐植物質学会, 佐賀大学, 国内会議土壌からHCl-DMSOで抽出される緑色色素について
口頭発表（一般）
- 日本腐植物質学会第１９回講演会講演要旨集, 2003年11月, 日本語, 日本腐植物質学会, 佐賀大学, 国内会議酵素処理による腐植酸およびフルボ酸の特性変化
口頭発表（一般）
- 日本土壌肥料学会2003年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議腐植構造特性のバリエーションと環境・生態系との関わり
口頭発表（一般）
- 日本水環境学会シンポジウム講演集, 2003年, 日本語, 日本水環境学会, 未記入, 国内会議琵琶湖水中の腐植物質の化学構造特性（元素分析・NMR 分光分析等）
口頭発表（一般）
- 日本土壌肥料学会2003 年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議土壌菌核粒子内に見られる生体鉱物について
口頭発表（一般）
- 日本土壌肥料学会2003 年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議土壌菌核様粒子の化学組成および化学結合状態について
口頭発表（一般）
- 日本土壌肥料学会2003 年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議神戸市周辺のため池に存在する水中有機物の特性および類型化
口頭発表（一般）
- 日本土壌肥料学会2003 年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議糸状菌の褪色作用に対する腐植の安定性
口頭発表（一般）
- 日本水環境学会シンポジウム講演集, 2003年, 日本語, 日本水環境学会, 未記入, 国内会議河川水及び地下水における腐植物質の特性
口頭発表（一般）
- Abstracts of the 13th annual V.M. Goldschmidt conference, 2003年, 英語, 未記入, Kurashiki, Japan, 国内会議Relation between biostability and chemical properties of soil humic substances
口頭発表（一般）
- 日本土壌肥料学会2003 年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議Effect of phosphate and pH on humification of three plant residues with fresh volcanic ashes
口頭発表（一般）
- 日本土壌肥料学会2003 年度大会講演要旨集, 2003年, 日本語, 日本土壌肥料学会, 神奈川, 国内会議A 型腐植酸様物質を含む土壌改良資材の創出（第三報）－創出したA 型腐植酸様物質の化学構造特性
口頭発表（一般）
共同研究・競争的資金等の研究課題
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 神戸大学, 2020年04月 - 2023年03月, 研究代表者北極モスツンドラ湿原の凍土融解・流出過程における有機炭素の分子種別変動機構の解明
- 科学研究費補助金／基盤研究(B), 2015年04月 - 2018年03月, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2015年04月 - 2018年03月
競争的資金
- 特別研究員奨励費, 2010年, 研究代表者
競争的資金
- 科学研究費補助金／萌芽研究, 2010年
競争的資金
- 科学研究費補助金／基盤研究(B), 2009年, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2005年, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2005年
競争的資金