研究者紹介システム
| 中屋敷 均 |  |
| ナカヤシキ ヒトシ | |
| 大学院農学研究科 生命機能科学専攻 | |
| 教授 | |
| 農学関係 |
研究者情報
所属
【主配置】
大学院農学研究科 生命機能科学専攻【配置】
農学部 生命機能科学科
学位
授業科目
- 生物学実験１, 国際教養教育院, 2021
- 生物学実験１, 国際教養教育院, 2021
- 生物学実験１, 国際教養教育院, 2021
- 生物学概論Ｄ２, 国際教養教育院, 2021
- 生物学実験２, 国際教養教育院, 2021
- 生物学実験２, 国際教養教育院, 2021
- 生物学実験２, 国際教養教育院, 2021
- ゲノム機能制御学, 大学院農学研究科, 2021
- ゲノム機能制御学１, 大学院農学研究科, 2021
- 特定課題演習 I-1, 大学院農学研究科, 2021
- 課題開発演習, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅰ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習I-2, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-1, 大学院農学研究科, 2021
- 特定課題演習Ⅱ－２, 大学院農学研究科, 2021
- 特定課題演習 I-2, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 先端遺伝科学, 大学院農学研究科, 2021
- 高度教養セミナー農学部環境生物学入門, 農学部, 2021
- 高度教養セミナー農学部環境生物学入門, 農学部, 2021
- 高度教養セミナー農学部環境生物学, 農学部, 2021
- 卒業研究, 農学部, 2021
- バイオサイエンス基礎英語１, 農学部, 2021
- 環境生物学実験II, 農学部, 2021
- 初年次セミナー（応用機能生物学コース）, 農学部, 2021
- 生物科学英語リスニング演習, 農学部, 2021
- バイオサイエンス基礎英語２, 農学部, 2021
- 緑の保全, 農学部, 2021
- 緑の保全の探究, 農学部, 2021
- 細胞生物学, 農学部, 2021
- 細胞生物学１, 農学部, 2021
- 生命機能科学各論II, 農学部, 2021
- 環境生物学実験I, 農学部, 2021
- 環境生物学実験III, 農学部, 2021
- 細胞生物学２, 農学部, 2021
ジャンル
コメントテーマ
研究活動
研究キーワード
研究分野
受賞
2016年09月 講談社, 講談社科学出版賞, ウイルスは生きている
日本国出版社・新聞社・財団等の賞
2015年08月 日本植物病理学会, 植物感染生理談話会ポスター発表優秀賞, Ralstonia solanacearum OE1-1株のバイオフィルム形成にレクチンES-IILは必要である
日本国国内学会・会議・シンポジウム等の賞
2011年 日本植物病理学会, 日本植物病理学会論文賞, Chuma, I., Shinogi, T., Hosogi, N., Ikeda, K., Nakayashiki, H., Park, P., and Tosa, Y. (2009) Cytological characteristics of microconidia of Magnaporthe oryzae. J. Gen. Plant Pathol. 75：353-358
学会誌・学術雑誌による顕彰
論文
- Springer Science and Business Media LLC, 2022年12月05日, Journal of General Plant Pathology, 89 (2), 77 - 83, 英語
[査読有り]
研究論文（学術雑誌）
- Wiley, 2022年08月11日, Molecular Plant Pathology, 23 (11), 1658 - 1670
[査読有り]
研究論文（学術雑誌）
Transposable elements are common targets for transcriptional and post-transcriptional gene silencing in eukaryotic genomes. However, the molecular mechanisms responsible for sensing such repeated sequences in the genome remain largely unknown. Here, we show that machinery of homologous recombination (HR) and RNA silencing play cooperative roles in copy number-dependent de novo DNA methylation of the retrotransposon MAGGY in the fungus Pyricularia oryzae. Genetic and physical interaction studies revealed that RecA domain-containing proteins, including P. oryzae homologs of Rad51, Rad55, and Rad57, together with an uncharacterized protein, Ddnm1, form complex(es) and mediate either the overall level or the copy number-dependence of de novo MAGGY DNA methylation, likely in conjunction with DNA repair. Interestingly, P. oryzae mutants of specific RNA silencing components (MoDCL1 and MoAGO2) were impaired in copy number-dependence of MAGGY methylation. Co-immunoprecipitation of MoAGO2 and HR components suggested a physical interaction between the HR and RNA silencing machinery in the process.
2021年03月19日, Communications biology, 4 (1), 351 - 351, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Eleusine isolates (members of the Eleusine pathotype) of Pyricularia oryzae are divided into two subgroups, EC-I and EC-II, differentiated by molecular markers. A multilocus phylogenetic analysis revealed that these subgroups are very close to Eragrostis isolates. EC-II and Eragrostis isolates were exclusively virulent on finger millet and weeping lovegrass, respectively, while EC-I isolates were virulent on both. The avirulence of EC-II on weeping lovegrass was conditioned by an avirulence gene, PWL1. All EC-II isolates shared a peculiar structure (P structure) that was considered to be produced by an insertion (or translocation) of a DNA fragment carrying PWL1. On the other hand, all EC-I and Eragrostis isolates were noncarriers of PWL1 and shared a gene structure that should have predated the insertion of the PWL1-containing fragment. These results, together with phylogenetic analyses using whole-genome sequences, suggest that the Eleusine-specific subgroup (EC-II) evolved through a loss of pathogenicity on weeping lovegrass caused by a gain of PWL1.
2020年02月, Molecular plant-microbe interactions : MPMI, 33 (2), 153 - 165, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
BACKGROUND: White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado 'Dusa' roots with that obtained from the fungus cultured in rich medium. RESULTS: The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. CONCLUSIONS: The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.
2019年12月26日, BMC genomics, 20 (1), 1016 - 1016, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5' terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.
2019年08月, Virology, 534, 25 - 35, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Pyricularia is a fungal genus comprising several pathogenic species causing the blast disease in monocots. Pyricularia oryzae, the best-known species, infects rice, wheat, finger millet, and other crops. As past comparative and population genomics studies mainly focused on isolates of P. oryzae, the genomes of the other Pyricularia species have not been well explored. In this study, we obtained a chromosomal-level genome assembly of the finger millet isolate P. oryzae MZ5-1-6 and also highly contiguous assemblies of Pyricularia sp. LS, P. grisea, and P. pennisetigena. The differences in the genomic content of repetitive DNA sequences could largely explain the variation in genome size among these new genomes. Moreover, we found extensive gene gains and losses and structural changes among Pyricularia genomes, including a large interchromosomal translocation. We searched for homologs of known blast effectors across fungal taxa and found that most avirulence effectors are specific to Pyricularia, whereas many other effectors share homologs with distant fungal taxa. In particular, we discovered a novel effector family with metalloprotease activity, distinct from the well-known AVR-Pita family. We predicted 751 gene families containing putative effectors in 7 Pyricularia genomes and found that 60 of them showed differential expression in the P. oryzae MZ5-1-6 transcriptomes obtained under experimental conditions mimicking the pathogen infection process. In summary, this study increased our understanding of the structural, functional, and evolutionary genomics of the blast pathogen and identified new potential effector genes, providing useful data for developing crops with durable resistance.
Oxford press, 2019年06月01日, Molecular biology and evolution, 36 (6), 1148 - 1161, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
© 2017 BSPP AND JOHN WILEY & SONS LTD After invasion into intercellular spaces of tomato plants, the soil-borne, plant-pathogenic Ralstonia solanacearum strain OE1-1 forms mushroom-shaped biofilms (mushroom-type biofilms, mBFs) on tomato cells, leading to its virulence. The strain OE1-1 produces aryl-furanone secondary metabolites, ralfuranones (A, B, J, K and L), dependent on the quorum sensing (QS) system, with methyl 3-hydroxymyristate (3-OH MAME) synthesized by PhcB as a QS signal. Ralfuranones are associated with the feedback loop of the QS system. A ralfuranone productivity-deficient mutant (ΔralA) exhibited significantly reduced growth in intercellular spaces compared with strain OE1-1, losing its virulence. To analyse the function of ralfuranones in mBF formation by OE1-1 cells, we observed cell aggregates of R. solanacearum strains statically incubated in tomato apoplast fluids on filters under a scanning electron microscope. The ΔralA strain formed significantly fewer microcolonies and mBFs than strain OE1-1. Supplementation of ralfuranones A, B, J and K, but not L, significantly enhanced the development of mBF formation by ΔralA. Furthermore, a phcB- and ralA-deleted mutant (ΔphcB/ralA) exhibited less formation of mBFs than OE1-1, although a QS-deficient, phcB-deleted mutant formed mBFs similar to OE1-1. Supplementation with 3-OH MAME significantly reduced the formation of mBFs by ΔphcB/ralA. The application of each ralfuranone significantly increased the formation of mBFs by ΔphcB/ralA supplied with 3-OH MAME. Together, our findings indicate that ralfuranones are implicated not only in the development of mBFs by strain OE1-1, but also in the suppression of QS-mediated negative regulation of mBF formation.
2018年04月, Molecular Plant Pathology, 19 (4), 975 - 985, 英語[査読有り]
研究論文（学術雑誌）
Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced in Δmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNA-mediated gene regulation pathways through a competition for sRNA.
2018年03月16日, Nucleic acids research, 46 (5), 2495 - 2508, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Lettuce big-vein disease, caused by Mirafiori lettuce big-vein virus and Lettuce big-vein associated virus, is suppressed when the pH of field soil becomes acidic. Therefore, we evaluated the effect of soil pH on the activities of Olpidium virulentus, the vector of the viruses. We found that acidic soil, pH less than 6.0, significantly reduced O. virulentus infection of the root and influenced the detection rate of zoospores released in the surrounding water. We concluded that acidic soil suppresses zoospore release from zoosporangia.
SPRINGER JAPAN KK, 2017年07月, JOURNAL OF GENERAL PLANT PATHOLOGY, 83 (4), 240 - 243, 英語[査読有り]
研究論文（学術雑誌）
We conducted laboratory experiments to assess the disease control effects of pH-decreasing fertilizer as a new control material for lettuce big-vein disease. Results show that the occurrence of big-vein disease was suppressed in concomitance with the descent of soil pH by applying the fertilizer.
関西病虫害研究会, 2017年05月, 関西病虫害研究会報, 59 (59), 51 - 53, 日本語[査読有り]
研究論文（学術雑誌）
The mechanism of colonization of intercellular spaces by the soil-borne and vascular plant-pathogenic bacterium Ralstonia solanacearum strain OE1-1 after invasion into host plants remains unclear. To analyse the behaviour of OE1-1 cells in intercellular spaces, tomato leaves with the lower epidermis layers excised after infiltration with OE1-1 were observed under a scanning electron microscope. OE1-1 cells formed microcolonies on the surfaces of tomato cells adjacent to intercellular spaces, and then aggregated surrounded by an extracellular matrix, forming mature biofilm structures. Furthermore, OE1-1 cells produced mushroom-type biofilms when incubated in fluids of apoplasts including intercellular spaces, but not xylem fluids from tomato plants. This is the first report of biofilm formation by R. solanacearum on host plant cells after invasion into intercellular spaces and mushroom-type biofilms produced by R. solanacearum in vitro. Sugar application led to enhanced biofilm formation by OE1-1. Mutation of lecM encoding a lectin, RS-IIL, which reportedly exhibits affinity for these sugars, led to a significant decrease in biofilm formation. Colonization in intercellular spaces was significantly decreased in the lecM mutant, leading to a loss of virulence on tomato plants. Complementation of the lecM mutant with native lecM resulted in the recovery of mushroom-type biofilms and virulence on tomato plants. Together, our findings indicate that OE1-1 produces mature biofilms on the surfaces of tomato cells after invasion into intercellular spaces. RS-IIL may contribute to biofilm formation by OE1-1, which is required for OE1-1 virulence.
WILEY-BLACKWELL, 2016年08月, MOLECULAR PLANT PATHOLOGY, 17 (6), 890 - 902, 英語[査読有り]
研究論文（学術雑誌）
- 日本植物病理学会, 2016年, 植物感染生理談話会論文集, (51), 83 - 94, 日本語糸状菌病害の組織特異的感染戦略 －いもち病菌の生い立ちを紐解く－
[招待有り]
研究論文（研究会，シンポジウム資料等）
We designed specific primers for Coniothyrium minitans, a sclerotia-parasitizing biocontrol agent (BCA), and quantified its biomass in the field by quantitative polymerase chain reaction. Because chemical properties of the soil can decrease the DNA extraction efficiency and DNA polymerase activity, standard curves were calibrated by adding known concentrations of BCA to sterilized field soil. The BCA biomass was monitored over time and after various treatments. Because the distribution of the BCA biomass was uneven in the field, a method to spread BCA uniformly in the field is needed.
SPRINGER JAPAN KK, 2016年01月, JOURNAL OF GENERAL PLANT PATHOLOGY, 82 (1), 51 - 56, 英語[査読有り]
研究論文（学術雑誌）
Hyphal interactions of 10 Rosellinia necatrix isolates were analyzed. Most incompatible pairings produced broad melanized barrage lines, with a few exceptions. The variation in barrage lines revealed using light microscopy might result from distinct frequencies of different hyphal interaction types. In incompatible pairings, hyphal anastomosis was rarely observed. Activated-charcoal treatment of oatmeal agar media prevented both incompatible and compatible reactions. FUN-1 staining showed vacuole dysfunction at hyphal interaction sites, and the localization of a Lifeact-GFP mutant revealed that actin dynamics was prominent in compatible pairings. These results suggested that self/nonself recognition commonly proceeded to vacuole dysfunction and that actin dynamics played a critical role in the restoration of vacuole integrity.
SPRINGER JAPAN KK, 2015年11月, JOURNAL OF GENERAL PLANT PATHOLOGY, 81 (6), 420 - 428, 英語[査読有り]
研究論文（学術雑誌）
- Phytopathological Society of Japan, 2015年09月, PSJ Plant-Microbe Interactions Symposium Report, (50), 11 - 21, 日本語Mechanisms of biofilm formation by Ralstonia solanacearum and its involvement in the bacterial virulence
[招待有り]
研究論文（研究会，シンポジウム資料等）
- Phytopathological Society of Japan, 2015年09月, PSJ Plant-Microbe Interactions Symposium Report, (50), 113 - 121, 日本語Epigenetic gene regulation in the rice blast fungus Pyricularia oryzae
[招待有り]
研究論文（研究会，シンポジウム資料等）
Here we report the genetic analyses of histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Magnaporthe oryzae. Eight putative M. oryzae KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various infection processes at varying degrees. Moset1 disruptants (Delta moset1) impaired in histone H3 lysine 4 methylation (H3K4me) showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation. Consequently,Delta moset1 lost pathogenicity on wheat host plants, thus indicating that H3K4me is an important epigenetic mark for infection-related gene expression in M. oryzae. Interestingly, appressorium formation was greatly restored in the Delta moset1 mutants by exogenous addition of cAMP or of the cutin monomer, 16-hydroxypalmitic acid. The Delta moset1 mutants were still infectious on the super-susceptible barley cultivar Nigrate. These results suggested that MoSET1 plays roles in various aspects of infection, including signal perception and overcoming host-specific resistance. However, since Delta moset1 was also impaired in vegetative growth, the impact of MoSET1 on gene regulation was not infection specific. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3) and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Delta moset1 mutant, led to the following conclusions: 1) Approximately 5% of M. oryzae genes showed significant changes in H3K4-me2 or -me3 abundance during infection-related morphogenesis. 2) In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 3) Lack of MoSET1 methyltransferase, however, resulted in up-regulation of a significant portion of the M. oryzae genes in the vegetative mycelia (1,491 genes), and during infection-related morphogenesis (1,385 genes), indicating that MoSET1 has a role in gene repression either directly or more likely indirectly. 4) Among the 4,077 differentially expressed genes (DEGs) between mycelia and germination tubes, 1,201 and 882 genes were up- and down-regulated, respectively, in a Moset1-dependent manner. 5) The Moset1-dependent DEGs were enriched in several gene categories such as signal transduction, transport, RNA processing, and translation.
PUBLIC LIBRARY SCIENCE, 2015年07月, PLOS GENETICS, 11 (7), e1005385, 英語[査読有り]
研究論文（学術雑誌）
Here we addressed the involvement of histone methylation at H3 lysine 4 (H3K4me) in substrate-dependent activation of cell-wall-degrading enzymes (CWDEs) in Magnaporthe oryzae. Transcriptional activation of 36 putative CWDEs including cellobiohydrolases, endoglucanases, beta-glucosidases, and endoxylanases was assessed by qRT-PCR. Among the CWDE genes, 16 were upregulated during infection and also in media containing their respective substrates with an exception. Interestingly, the substrate-dependent activation of most CWDE genes was severely impaired in a deletion mutant of MoSET1, histone H3K4 methyltransferase in M. oryzae, suggesting that MoSET1 directly or indirectly regulates a wide range of CWDE genes.
SPRINGER JAPAN KK, 2015年03月, JOURNAL OF GENERAL PLANT PATHOLOGY, 81 (2), 127 - 130, 英語[査読有り]
研究論文（学術雑誌）
- 日本土壌微生物学会, 2014年, 土と微生物, 68 (1), 50 - 50, 日本語
[査読有り]
The mechanisms involved in substrate-dependent regulation of a Magnaporthe oryzae gene encoding a cellulase which we designate MoCel7C (MGG_14954) were investigated. The levels of MoCel7C transcript were dramatically increased more than 1,000-fold, 16 to 24 h after transfer to a medium containing 2% carboxymethylcellulose (CMC), while levels were very low or undetectable in conventional rich medium. Green fluorescent protein reporter assays showed that the MoCel7C promoter was activated by cello-oligosaccharides larger than a pentamer. CMC-induced activation of the MoCel7C promoter was suppressed by glucose and cellobiose. Chromatin immunoprecipitation assays revealed that histone H3 methylation on lysine 4 (H3K4) at the MoCel7C locus was associated with activation of the gene by CMC. Consistently, CMC-induced MoCel7C gene activation was drastically diminished in a knockout (KO) mutant of the MoSET1 gene, which encodes a histone lysine methyltransferase that catalyzes H3K4 methylation in M. oryzae. Interestingly, however, MoCel7C transcript levels under noninducing conditions were significantly increased in the MoSET1 KO mutant, suggesting that MoSET1 directly or indirectly plays a role in both activation and suppression of the MoCel7C gene in response to environmental signals. In addition, gene expression and silencing vectors using the MoCel7C promoter were constructed.
AMER SOC MICROBIOLOGY, 2013年11月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79 (21), 6823 - 6832, 英語[査読有り]
研究論文（学術雑誌）
The long terminal repeat retrotransposon, Magnaporthe gypsy-like element (MAGGY), has been shown to be targeted for cytosine methylation in a subset of Magnaporthe oryzae field isolates. Analysis of the F-1 progeny from a genetic cross between methylation-proficient (Br48) and methylation-deficient (GFSI1-7-2) isolates revealed that methylation of the MAGGY element was governed by a single dominant gene. Positional cloning followed by gene disruption and complementation experiments revealed that the responsible gene was the DNA methyltransferase, MoDMT1, an ortholog of Neurospora crassa Dim-2. A survey of MAGGY methylation in 60 Magnaporthe field isolates revealed that 42 isolates from rice, common millet, wheat, finger millet, and buffelgrass were methylation proficient while 18 isolates from foxtail millet, green bristlegrass, Japanese panicgrass, torpedo grass, Guinea grass, and crabgrass were methylation deficient. Phenotypic analyses showed that MoDMT1 plays no major role in development and pathogenicity of the fungus. Quantitative polymerase chain reaction analysis showed that the average copy number of genomic MAGGY elements was not significantly different between methylation-deficient and -proficient field isolates even though the levels of MAGGY transcript were generally higher in the former group. MoDMT1 gene sequences in the methylation-deficient isolates suggested that at least three independent mutations were responsible for the loss of MoDMT1 function. Overall, our data suggest that MoDMT1 is not essential for the natural life cycle of the fungus and raise the possibility that the genus Magnaporthe may be losing the mechanism of DNA methylation on the evolutionary time scale.
GENETICS SOC AM, 2013年11月, GENETICS, 195 (3), 845 - +, 英語[査読有り]
研究論文（学術雑誌）
Black spot disease, Alternaria alternataJapanese pear pathotype, produces the host-specific toxin AK-toxin, an important pathogenicity factor. Previously, we have found that hydrogen peroxide is produced in the hyphal cell wall at the plantpathogen interaction site, suggesting that the fungal reactive oxygen species (ROS) generation machinery is important for pathogenicity. In this study, we identified two NADPH oxidase (NoxA and NoxB) genes and produced nox disruption mutants. noxA and noxB disruption mutants showed increased hyphal branching and spore production per unit area. Surprisingly, only the noxB disruption mutant compromised disease symptoms. A fluorescent protein reporter assay revealed that only NoxB localized at the appressoria during pear leaf infection. In contrast, both NoxA and NoxB were highly expressed on the cellulose membrane, and these Nox proteins were also localized at the appressoria. In the noxB disruption mutant, we could not detect any necrotic lesions caused by AK-toxin. Moreover, the noxB disruption mutant did not induce papilla formation on pear leaves. Ultrastructural analysis revealed that the noxB disruption mutant also did not penetrate the cuticle layer. Moreover, ROS generation was not essential for penetration, suggesting that NoxB may have an unknown function in penetration. Taken together, our results suggest that NoxB is essential for aggressiveness and basal pathogenicity in A.alternata.
WILEY-BLACKWELL, 2013年05月, Molecular Plant Pathology, 14 (4), 365 - 378, 英語[査読有り]
研究論文（学術雑誌）
Victorin, the host-selective toxin produced by the fungus Cochliobolus victoriae, induces programmed cell death (PCD) in victorin-sensitive oat lines with characteristic features of animal apoptosis, such as mitochondrial permeability transition, chromatin condensation, nuclear DNA laddering and rRNA/mRNA degradation. In this study, we characterized a calcium-binding protein, namely AsALG-2, which might have a role in the victorin-induced PCD. AsALG-2 is homologous to the Apoptosis-Linked Gene ALG-2 identified in mammalian cells. Northern blot analysis revealed that the accumulation of AsALG-2 transcripts increased during victorin-induced PCD, but not during necrotic cell death. Salicylic acid, chitosan and chitin strongly activated the expression of general defence response genes, such as PR-10; however, neither induced cell death nor the accumulation of AsALG-2 mRNA. Pharmacological studies indicated that victorin-induced DNA laddering and AsALG-2 expression were regulated through similar pathways. The calcium channel blocker, nifedipine, moderately inhibited the accumulation of AsALG-2 mRNA during cell death. Trifluoperazine (calmodulin antagonist) and K252a (serine-threonine kinase inhibitor) reduced the victorin-induced phytoalexin accumulation, but did not prevent the victorin-induced DNA laddering or accumulation of AsALG-2 mRNA. Taken together, our investigations suggest that there is a calcium-mediated signalling pathway in animal and plant PCD in common.
WILEY-BLACKWELL, 2013年04月, MOLECULAR PLANT PATHOLOGY, 14 (3), 222 - 229, 英語[査読有り]
研究論文（学術雑誌）
Upon infection, phytopathogenic fungi secrete an array of hydrolytic enzymes that can degrade components of the host epidermis, including waxes, the cuticle, and cell walls. Cellulases, which can hydrolyze crystalline cellulose in the plant cell wall, are among these hydrolytic enzymes. Here, we provide RNAi-based evidence to show that cellulases belonging to glycosyl hydrolase (GH) families 6 and 7 contribute to the penetration of the host epidermis and further invasion by the phytopathogenic fungus Magnaporthe oryzae. The GH6 and GH7 cellulases likely include all members of the cellobiohydrolase family and some endoglucanases in M. oryzae. Quantitative reverse-transcriptase polymerase chain reaction analysis indicated that more than half of the cellulases were highly induced during infection. We constructed knock-down (KD) mutants of these cellulases using the building blocks method we reported previously. The transcript levels of the target genes and cellulase activity were considerably reduced in the KD mutants. The KD mutants resulted in fewer lesions, less penetration, and infection of fewer cells compared with the parent strain. Cytological analyses showed that a high rate of papilla formation blocked invasion of the KD mutants into host cells. These results suggest that the GH6 and GH7 cellulases play roles in the virulence of M. oryzae.
AMER PHYTOPATHOLOGICAL SOC, 2012年09月, MOLECULAR PLANT-MICROBE INTERACTIONS, 25 (9), 1135 - 1141, 英語[査読有り]
研究論文（学術雑誌）
Pseudomonas cichorii harbors the hrp genes. hrp-mutants lose their virulence on eggplant but not on lettuce. A phosphinothricin N-acetyltransferase gene (pat) is located between hrpL and an aldehyde dehydrogenase gene (aldH) in the genome of P. cichorii. Comparison of nucleotide sequences and composition of the genes among pseudomonads suggests a common ancestor of hrp and pat between P. cichorii strains and P. viridiflava strains harboring the single hrp pathogenicity island. In contrast, phylogenetic diversification of aldH corresponded to species diversification amongst pseudomonads. In this study, the involvement of aldH and pat in P. cichorii virulence was analyzed. An aldH-deleted mutant (Delta aldH) and a pat-deleted mutant (Delta pat) lost their virulence on eggplant but not on lettuce. P. cichorii expressed both genes in eggplant leaves, independent of HrpL, the transcriptional activator for the hrp. Inoculation into Asteraceae species susceptible to P. cichorii showed that the involvement of hrp, pat and aldH in P. cichorii virulence is independent of each other and has no relationship with the phylogeny of Asteraceae species based on the nucleotide sequences of ndhF and rbcL. It is thus thought that not only the hrp genes but also pat and aldH are implicated in the diversity of P. cichorii virulence on susceptible host plant species.
MDPI AG, 2012年03月, GENES, 3 (1), 62 - 80, 英語[査読有り]
研究論文（学術雑誌）
Pseudomonas fluorescens FPT9601株は,トマトにおいて植物成長促進根圏細菌として働き,ナス科植物青枯れ病菌に対する抵抗性を付与することで単離同定された。本菌はモデル植物であるシロイヌナズナにおいても,誘導全身抵抗性を引き起こす。シロイヌナズナのサリチル酸(SA),ジャスモン酸(JA)及びエチレン(ET)経路の変異株を用いて調査を行った所,FPT9601株が誘導する病害抵抗性にJAおよびET経路の関与が示唆された。次に約23.000遺伝子のプローブセットによるマイクロアレイ解析を行った結果,2倍以上の発現変動で上昇した遺伝子が95個,低下したものが105個であった。その中にオーキシン応答性遺伝子の発現増高が認められ,本菌による植物の成長促進はオーキシンの作用であることが示唆された。この他に二次代謝経路に関わる遺伝子の誘導や抑制,タンパク質合成系遺伝子の抑制などの傾向が認められた。また根粒菌感染で発現が増高することから命名されたノジュリン遺伝子群に含まれる遺伝子の誘導なども見られた。マイクロアレイ解析の結果,FPT9601感染により発現が上昇していた遺伝子のトランスポゾン挿入変異体におけるFPT9601株による誘導全身抵抗性について調査した所,転写因子などいくつかの変異体において,誘導抵抗性の低下が認められた。
土壌微生物研究会, 2012年, 土と微生物, 66 (2), 43 - 46, 日本語We determined the temporal and spatial localization of the phytoalexin avenanthramide A, and its biosynthetic enzyme, hydroxycinnamoyl-CoA: hydroxyanthranilate N-hydroxy-cinnamoyltransferase (AsHHT) in oat leaves infected with the crown rust fungus. Accumulation of avenanthramide A and AsHHT was first observed predominantly in hypersensitive response (HR) cells at 36 h post inoculation (hpi), and later in adjacent cells at 48 hpi. At 120 hpi, avenanthramide A was detected in a wider area of infected tissues, but AsHHT-positive signals were only observed in the HR and adjacent cells, suggesting that avenanthramide A was synthesized around the HR cells, and then transported to other parts of the infected tissues in a center-to-periphery manner. (C) 2011 Elsevier Ltd. All rights reserved.
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2011年12月, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 76 (3-4), 173 - 181, 英語[査読有り]
研究論文（学術雑誌）
Due to functional redundancy, it is often difficult to genetically analyse the biological function of fungal cell wall-degrading enzymes that belong to a gene family. To overcome this difficulty, we used RNAi to knock-down (KD) multiple xylanase genes to eluci-date their roles in the pathogenicity of the blast fungus, Magnaporthe oryzae. To obtain the maximum average efficiency of gene silencing for the xylanase genes, we used the 'building blocks method', in which a 40 bp sequence was chosen from an endoxylanase gene, and 10 such sequences from 10 endoxylanases were combined to make an artificial RNAi trigger by synthetic DNA. Quantitative RT-PCR analysis revealed that the transcript levels of all the expressed xylanase genes were significantly reduced in KD mutants with the artificial RNAi trigger. Even though the KD mutants did not completely lose their pathogenicity to host plants, the number of lesions, rate of penetration and extent of infected cells were all reduced in KD mutant-infected leaves. The degree of pathogenicity reduction was associated with the silencing levels of xylanase mRNA and enzymatic activity in the KD mutants. Cytological analysis indicated that xylanases play significant roles in both vertical penetration and horizontal expansion of M. oryzae in infected plants.
WILEY-BLACKWELL, 2011年08月, Mol. Microbiol., 81 (4), 1008 - 1019, 英語[査読有り]
研究論文（学術雑誌）
Magnaporthe oryzae is the causal agent of rice blast disease, a devastating problem worldwide. This fungus has caused breakdown of resistance conferred by newly developed commercial cultivars. To address how the rice blast fungus adapts itself to new resistance genes so quickly, we examined chromosomal locations of AVR-Pita, a subtelomeric gene family corresponding to the Pita resistance gene, in various isolates of M. oryzae (including wheat and millet pathogens) and its related species. We found that AVR-Pita (AVR-Pita1 and AVR-Pita2) is highly variable in its genome location, occurring in chromosomes 1, 3, 4, 5, 6, 7, and supernumerary chromosomes, particularly in rice-infecting isolates. When expressed in M. oryzae, most of the AVR-Pita homologs could elicit Pita-mediated resistance, even those from non-rice isolates. AVR-Pita was flanked by a retrotransposon, which presumably contributed to its multiple translocation across the genome. On the other hand, family member AVR-Pita3, which lacks avirulence activity, was stably located on chromosome 7 in a vast majority of isolates. These results suggest that the diversification in genome location of AVR-Pita in the rice isolates is a consequence of recognition by Pita in rice. We propose a model that the multiple translocation of AVR-Pita may be associated with its frequent loss and recovery mediated by its transfer among individuals in asexual populations. This model implies that the high mobility of AVR-Pita is a key mechanism accounting for the rapid adaptation toward Pita. Dynamic adaptation of some fungal plant pathogens may be achieved by deletion and recovery of avirulence genes using a population as a unit of adaptation.
PUBLIC LIBRARY SCIENCE, 2011年07月, PLOS PATHOGENS, 7 (7), e1002147, 英語[査読有り]
研究論文（学術雑誌）
Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3' UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing assay with a GFP gene revealed that pSilent-MG vectors induced gene silencing at a practical level. In the resulting GFP-silenced transformants, GFP siRNAs accumulated and DNA methylation increased in the GFP-expressing gene, implying that the vector could induce both transcriptional and post-transcriptional gene silencing.
SPRINGER TOKYO, 2011年05月, JOURNAL OF GENERAL PLANT PATHOLOGY, 77 (3), 147 - 151, 英語[査読有り]
研究論文（学術雑誌）
The ethyl acetate extract of the conidia germination fluid from an Avena isolate (Br58) of Pyricularia oryzae had chlorosis-inducing activity on oat leaf segments. The same activity was also present in the acetone extract of an oatmeal agar culture of Br58. Fungal cultures were used for a large-scale preparation. A series of acetone and ethyl acetate extraction monitored by chromatography was used to isolate an active fraction. The active principle was purified by HPLC. We show by NAIR and LC/MS that the toxin was an oxidized C18 unsaturated fatty acid named Mag-toxin. Mag-toxin induced chlorosis on oat leaf segments incubated in the light but not in the dark. Reactive oxygen species (ROS) and cell death were induced by Mag-toxin in oat cells. The subcellular localization of ROS generation induced by the toxin treatment was correlated with the location of mitochondria. Interestingly, the induction of ROS generation and cell death by Mag-toxin was light-independent.
TAYLOR & FRANCIS LTD, 2010年11月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74 (11), 2220 - 2225, 英語[査読有り]
研究論文（学術雑誌）
Understanding the molecular basis of plant responses to pathogen-associated molecular patterns (PAMPs) is an active area of research in the field of plant microbe interactions. A growing number of plant genes involved in various steps of PAMP-triggered immunity (PTI) pathways and microbial factors involved in the elicitation or suppression of PTI have been identified. These studies have largely relied on Arabidopsis thaliana and, therefore, most of the PTI assays have been developed and optimized for that model plant system. Although PTI is a conserved feature among plants, the response spectra vary across different species. Thus, there is a need for robust PTI assays in other pathosystems, such as those involving Solanaceae plant pathogen interactions, which include many economically important plants and their diseases. We have optimized molecular, cellular, and whole-plant methods to measure PTI responses in two widely studied solanaceous species, tomato (Solanum lycopersicum) and Nicotiana benthamiana. Here, we provide detailed protocols for measuring various PTI-associated phenotypes, including bacterial populations after pretreatment of leaves with PAMPs, induction of reporter genes, callose deposition, activation of mitogen-activated protein kinases, and a luciferase-based reporter system. These methods will facilitate limited genetic screens and detailed characterization of potential PTI-related genes in model and economically important Solanaceae spp.-pathogen interactions.
AMER PHYTOPATHOLOGICAL SOC, 2010年08月, MOLECULAR PLANT-MICROBE INTERACTIONS, 23 (8), 991 - 999, 英語[査読有り]
研究論文（学術雑誌）
Eleusine isolates (members of the Eleusine subgroup) of Pyricularia oryzae are divided into two groups, Ec-I and Ec-II, differentiated by molecular markers. A multilocus phylogenetic analysis and DNA fingerprinting suggested that Ec-I isolates are very close to Eragrostis isolates rather than Ec-II isolates. Infection assays revealed that Ec-II and Eragrostis isolates were exclusively virulent on finger millet and weeping lovegrass, respectively, whereas Ec-I isolates were virulent on both. The avirulence or virulence on weeping lovegrass perfectly corresponded to the presence or absence of an avirulence gene, PWL1; all Ec-II isolates carried an identical, functional PWL1, whereas none of Ec-I isolates or Eragrostis isolates carried it. A comparison of PWL1 flanking regions revealed that Ec-II isolates had a peculiar structure produced by an insertion (or translocation) of a DNA fragment carrying PWL1. Based on these results, a model was constructed which illustrated possible pathways to the establishment of the Eleusine subgroup.
AMER PHYTOPATHOLOGICAL SOC, 2010年06月, MOLECULAR PLANT-MICROBE INTERACTIONS, 23 (6), 771 - 783, 英語[査読有り]
研究論文（学術雑誌）
- 2010年, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 86, 英語Pathological roles of reactive oxygen species in plant pathogen
研究論文（国際会議プロシーディングス）
The inner cellular structure of microconidia of Magnaporthe oryzae was examined using fluorescent probes and electron microscopic techniques. The volume of the nucleus relative to the cell was significantly larger in microconidia than in macroconidia or vegetative hyphae, similar to observations for spermatia of other fungi. Selective fluorescent staining revealed that cytosolic RNA was less abundant in microconidia than in macroconidia and germ tubes, suggesting that general metabolic activity in microconidia is low. Consistently, GFP expression driven by the TrpC promoter was highly active during the formation of phialides and microconidia but gradually decreased as the microconidia matured. Such data suggest that microconidia are in a quiescent or dormant state.
SPRINGER TOKYO, 2009年10月, JOURNAL OF GENERAL PLANT PATHOLOGY, 75 (5), 353 - 358, 英語[査読有り]
研究論文（学術雑誌）
Approximately 30,000 fluorescent bacterial strains isolated from tomato, lettuce, eggplant, Chinese cabbage, and Japanese pepper plants at seven different locations in Hyogo Prefecture, were screened for plant-growth-promoting (PGP) activity to induce disease resistance against bacterial wilt in tomato. The 37 strains that had higher PGP activity were subjected to molecular phylogenetic analyses using the sequences of the 16S rRNA, gyrB and rpoD genes. Most of the strains were identified as Pseudomonas fluorescens or its close relative, P. putida, while a few strains were grouped with more distantly related bacterial species such as Enterobacter and Stenotrophomonas. The phylogenetic relationships among tomato and lettuce isolates mostly coincided with the source locality and host plants, with a few exceptions. In contrast, isolates from Japanese pepper plants did not form their own cluster but represented several different bacterial species.
SPRINGER TOKYO, 2009年08月, JOURNAL OF GENERAL PLANT PATHOLOGY, 75 (4), 316 - 321, 英語[査読有り]
研究論文（学術雑誌）
Population structure of Eleusine isolates of Pyricularia oryzae (Magnaporthe oryzae) was examined using DNA markers. On the basis of rDNA sequences, Eleusine isolates were divided into two groups. One group clustered with Triticum isolates, while the other clustered with Eragrostis isolates. This grouping was supported by DNA fingerprinting with three repetitive elements: MGR586, MGR583, and grasshopper. These results suggest that the population of Eleusine isolates is composed of at least two groups that evolved independently from the original population of P. oryzae. Most of the isolates that were collected just after an outbreak of finger millet blast in the 1970s had almost identical fingerprint profiles although they were collected in distant prefectures. This result supports the idea that the outbreak was caused by seed transmission of a particular strain of Eleusine isolates.
SPRINGER TOKYO, 2009年06月, JOURNAL OF GENERAL PLANT PATHOLOGY, 75 (3), 173 - 180, 英語[査読有り]
研究論文（学術雑誌）
For over a hundred years, it has been discussed whether or not Pyricularia isolates on rice (Oryza sativa) and on crabgrass (Digitaria sanguinalis) belong to the same species, Pyricularia grisea (teleomorph, Magnaporthe grisea). We thought that pyrichalasin H, isolated from a Digitaria isolate, could be used as a marker of the chemotaxonomy of Pyricularia isolates. Pyrichalasin H was only detected in culture filtrates of Pyricularia isolates to infect Digitaria plants among 72 isolates from 20 species of gramineous plants. There was a correlation between pyrichalasin H production and the ability of Pyricularia isolates to infect Digitaria. Pre-treatment of leaf sheaths of crabgrass with pyrichalasin H led to penetration and colonization by nonhost isolates. Thus, we propose that pyrichalasin H may be responsible for the specific pathogenicity of Pyricularia isolates on the Digitaria genus. This result also supports the concept that isolates from rice and other cereals should be referred to as Magnaporthe oryzae and isolates from Digitaria should be referred to as M. grisea. The blast fungus produces necrotic lesions on its original host plant. These lesions might be formed by phytotoxins of blast fungus. Some necrosis-inducing factors have been isolated from Pyricularia isolates. However, their toxins have not been compared to their quantities in cultures of Pyricularia isolates and their activity on host plants. We searched for the, necrosis-inducing factor from a Triticum isolate pathogenic on wheat (Triticum aestivum). Pyriculol and epipyriculol were detected as the main necrosis-inducing factors.
SPRINGER, 2009年, ADVANCES IN GENETICS, GENOMICS AND CONTROL OF RICE BLAST DISEASE, 247 - +, 英語[査読有り]
研究論文（国際会議プロシーディングス）
Pseudomonas cichorii is the major causal agent of bacterial rot disease in lettuce, and apoptosis-like programmed cell death is closely associated with disease development. Depletion of cellular ATP and expression of the alternative oxidase gene was observed in lettuce leaves inoculated with P. cichorii suggesting mitochondrial dysfunction. Cytochemical observation showed production of reactive oxygen species in mitochondria of P. cichorii-inoculated lettuce leaf cells. Release of cytochrome c into the cytosol was also induced by inoculation with the bacterium. Superoxide production was observed in the mitochondrial fraction isolated from P. cichorii-infected leaves much more intense than water-treated leaves. Loss of swelling ability was also observed in the mitochondrial fractions following inoculation with P. cichorii. Intriguingly, inhibitors of the mitochondrial respiratory complex III prevented loss of swelling ability, whereas superoxide generation was scarcely affected by inhibitors of mitochondrial permeability transition in the mitochondrial fractions. Inhibitors of the mitochondrial respiratory complex III and mitochondrial permeability transition delayed not only P. cichorii-induced cell death, but also disease development. In contrast, P. cichorii-induced DNA fragmentation was not inhibited in the presence of either type of inhibitor. The findings suggest that mitochondria may play a crucial role in DNA fragmentation-independent cell death pathway(s). (C) 2009 Elsevier Ltd. All rights reserved.
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2009年01月, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 74 (1), 45 - 54, 英語[査読有り]
研究論文（学術雑誌）
Quantitative RT-PCR and overexpression studies of two Dicer-like proteins, MoDcll and MoDcl2, in Magnaporthe oryzae indicated that the functional diversification of the MoDcll and MoDcl2 proteins in RNA-mediated gene silencing pathways was likely to have arisen from both transcriptional control and protein specialization.
GENETICS, 2008年10月, GENETICS, 180 (2), 1245 - 1249, 英語[査読有り]
研究論文（学術雑誌）
We developed an RNA-silencing vector, pSilent-Dual1 (pSD1), with a convergent dual promoter system that provides a high-throughput platform for functional genomics research in filamentous fungi. In the pSD1 system, the target gene was designed to be transcribed as a chimeric RNA with enhanced green fluorescent protein (eGFP) RNA. This enabled us to efficiently screen the resulting transformants using GFP fluorescence as an indicator of gene silencing. A model study with the eGFP gene showed that pSD1-based vectors induced gene silencing via the RNAi pathway with slightly lower efficiency than did hairpin eGFP RNA-expressing vectors. To demonstrate the applicability of the pSD1 system for elucidating gene function in the rice-blast fungus Magnaporthe oryzae, 37 calcium signalling-related genes that include almost all known calcium-signalling proteins in the genome were targeted for gene silencing by the vector. Phenotypic analyses of the silenced transformants showed that at least 26, 35 and 15 of the 37 genes examined were involved in hyphal growth, sporulation and pathogenicity, respectively, in M. oryzae. These included several novel findings such as that Pmc1-, Spf1- and Neo1-like Ca2+ pumps, calreticulin and calpactin heavy chain were essential for fungal pathogenicity.
WILEY, 2008年06月, MOLECULAR MICROBIOLOGY, 68 (6), 1348 - 1365, 英語[査読有り]
研究論文（学術雑誌）
- 2008年, Korean Journal of Microscopy, 38, 1235 - 1236, 英語Pathological roles of reactive oxygen species at penetration pegs of Alternaria Japanese pear pathotype during plant infection
研究論文（国際会議プロシーディングス）
The LTR-retrotransposon MAGGY was introduced into naive genomes of Magnaporthe oryzae with different genetic backgrounds (wild-type, and MoDcl1 [mdl1] and MoDcl2 [mdl2] dicer mutants). The MoDcl2 mutants deficient in MAGGY siRNA biogenesis generally showed greater MAGGY mRNA accumulation and more rapid increase in MAGGY copy number than did the wild-type and MoDcl1 mutants exhibiting normal MAGGY siRNA accumulation, indicating that RNA silencing functioned as an effective defense against the invading element. Interestingly, however, regardless of genetic background, the rate of MAGGY transposition drastically decreased as its copy number in the genome increased. Notably, in the MoDcl2 mutant, copy-number-dependent MAGGY suppression occurred without a reduction in its mRNA accumulation, and therefore by a silencing mechanism distinct from both transcriptional gene silencing and siRNA-mediated RNA silencing. This might imply that some mechanism possibly similar to post-transcriptional cosuppression of Ty1 retrotransposition in Saccharomyces cerevisiae, which operates regardless of the abundance of target transcript and independent of RNA silencing, would also function in M. oryzae that possesses the RNA silencing machinery.
OXFORD UNIV PRESS, 2007年09月, NUCLEIC ACIDS RESEARCH, 35 (18), 5987 - 5994, 英語[査読有り]
研究論文（学術雑誌）
Pyricularia isolates from various host plants were subjected to a multilocus phylogenetic analysis based on rDNA-ITS, actin, beta-tubulin, and calmodulin loci. A combined gene tree resolved seven groups with 100% BS support, suggesting that they are monophyletic groups supported concordantly by all four loci. By incorporating biological and morphological species criteria, each of the seven groups was considered to be a current species. However, phylogenetic relationships among these species were unresolved in the single-gene trees and in the combined tree. Furthermore, the transition from concordance to conflict occurred more than once in the combined gene tree. They were interpreted by assuming that Pyricularia has evolved through repeated species radiation. The transition point other than the current species limit was considered to be the limit of the former species. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2007年07月, MYCOLOGICAL RESEARCH, 111 (Pt 7), 799 - 808, 英語[査読有り]
研究論文（学術雑誌）
- 2007年, J. Gen. Plant Pathol., 73, 22 - 28
[査読有り]
Comprehensive phylogenetic analyses of fungal Argonaute, Dicer, and RNA-dependent RNA polymerase-like proteins have been performed to gain insights into the diversification of RNA silencing pathways during the evolution of fungi. A wide range of fungi including ascomycetes, basidiomycetyes, and zygomycetes possesses multiple RNA silencing components in the genome, whereas a portion of ascomycete and basidiomycete fungi apparently lacks the whole or most of the components. The number of paralogous silencing proteins in the genome differs considerably among fungal species, suggesting that RNA silencing pathways have diversified significantly during evolution in parallel with developing the complexity of life cycle or in response to environmental conditions. Interestingly, orthologous silencing proteins from different fungal clades are often clustered more closely than paralogous proteins in a fungus, indicating that duplication events occurred before speciation events. Therefore, the origin of multiple RNA silencing pathways seems to be very ancient, likely having occurred prior to the divergence of the major fungal lineages.
SPRINGER, 2006年07月, JOURNAL OF MOLECULAR EVOLUTION, 63 (1), 127 - 135, 英語[査読有り]
研究論文（学術雑誌）
Here we report that rRNA and mRNA are specifically degraded in oat (Avena sativa L.) cells during apoptotic cell death induced by victorin, a host-selective toxin produced by Cochliobolus victoriae. Northern analysis indicated that rRNA species from the cytosol, mitochondria and chloroplasts were all degraded via specific degradation intermediates during victorin-induced apoptotic cell death but, in contrast, they were randomly digested in necrotic cell death induced by 30 MM CuSO4 and heat shock. This indicates that specific rRNA cleavage could be controlled by an intrinsic program. We also observed specific cleavage of mRNA of housekeeping genes such as actin and ubiquitin during victorin-induced cell death. Interestingly, no victorin-induced mRNA degradation was detected with stress-responding genes such as PR-1, PR-10 and GPx throughout the experimental period. The RNA degradation mostly, but not always, occurred in parallel with DNA laddering, but pharmacological studies indicated that these processes are regulated by different signaling pathways with some overlapping upstream signals.
BLACKWELL PUBLISHING, 2006年06月, PLANT JOURNAL, 46 (6), 922 - 933, 英語[査読有り]
研究論文（学術雑誌）
Pseudomonas cichorii is the major causal agent of bacterial rot of lettuce. Collapse and browning symptoms were observed in lettuce leaf tissue from 15 to 24 h after inoculation (HAI) with P cichorii; superoxide anion generation was detected at I to 6 HAI; and cell death was induced at 6 HAI, reaching a maximum at approximately 9 and 12 HAI. Heterochromatin condensation and DNA laddering also were observed within 3 HAI. Pharmacological studies showed that induction of cell death and DNA laddering was closely associated with de novo protein synthesis, protein kinase, intracellular reactive oxygen species, DNase, serine protease, and caspase III-like protease. Moreover, chemicals, which inhibited the induction of cell death and DNA laddering, also suppressed the development of disease symptoms. These results suggest that apoptotic cell death might be closely associated with the development of bacterial rot caused by P cichorii.
AMER PHYTOPATHOLOGICAL SOC, 2006年02月, MOLECULAR PLANT-MICROBE INTERACTIONS, 19 (2), 112 - 122, 英語[査読有り]
研究論文（学術雑誌）
Reactive oxygen species (ROS) are thought to be involved in various forms of programmed cell death (PCD) in animal and plant cells. PCD, along with the production of ROS, occurs during plant-pathogen interactions. Here we show that victorin, a host-specific toxin produced by Cochliobolus victoriae, which causes victoria blight of oats, induces two phases of intracellular ROS production in victorin-sensitive oat mesophyll cells. The initial production of ROS is restricted at mitochondria and not accompanied with cellular oxidative damage. Later production of ROS is dispersed into cells concomitant with lipid peroxidation, chloroplast dysfunction, and cell death. Superoxide dismutase can clearly suppress the initial ROS production and delay the progression of cell death. These data indicate that the initial ROS production may be involved in the cell death induction process, and the later ROS production may play important roles in events leading to cellular disruption. © The Phytopathological Society of Japan and Springer-Verlag 2005.
2005年12月, Journal of General Plant Pathology, 71 (6), 387 - 394, 英語[査読有り]
研究論文（学術雑誌）
Culture filtrates from 72 isolates of Pyricularia, grouped into 13 rDNA types, were analysed via HPLC. Of these isolates, 31 (r9 DNA type) from crabgrass (Digitaria sanguinalis), one (r9 DNA type) from pangolagrass (Digitaria smutsii) and six (r8 DNA type) from Digitaria horizontalis produced 20-280 mu g pyrichalasin H per millilitre of culture. These same isolates were pathogenic on five Digitaria species. Interestingly, two isolates, KM-1 and Br 29, which were originally isolated from Digitaria plants, did not produce pyrichalasin H, nor caused blast lesion on Digitaria plants. Because these two isolates were identified as Digitaria pathogens by PCR analysis using Digitaria-specific primers, they are likely to be mutants lacking pyrichalasin H production. Isolates that belonged to the remaining 11 rDNA types did not produce pyrichalasin H and were avirulent to Digitaria plants. Therefore, the virulence of Pyricularia on Digitaria plants correlates with pyrichalasin H production. Pyrichalasin H was also present in spore germination fluid of a crabgrass isolate (IBDS 5-1-1), but not in that of isolates from rice, foxtail millet, finger millet, common millet and wheat. In addition, pyrichalasin H was detected in host leaves infected with IBDS 5-1-1, but not in leaves from other plants infected with compatible Pyricularia isolates. Pretreatment of leaf sheaths of crabgrass with 3 mu g/mL pyrichalasin H led to the penetration and colonization by non-host isolates. Overall, these results indicate that production of pyrichalasin H is responsible for the genus-specific pathogenicity of Digitaria isolates.
WILEY-BLACKWELL, 2005年11月, MOLECULAR PLANT PATHOLOGY, 6 (6), 605 - 613, 英語[査読有り]
研究論文（学術雑誌）
The significance of AVR1-CO39, an avirulence gene of the blast fungus corresponding to Pi-CO39(t) in rice cultivars, during the evolution and differentiation of the blast fungus was evaluated by studying its function and distribution in Pyricularia spp. When the presence or absence of AVR1-C039 was plotted on a dendrogram constructed from ribosomal DNA sequences, a perfect parallelism was observed between its distribution and the phylogeny of Pyricularia isolates. AVR1-CO39 homologs were exclusively present in one species, Pyricularia oryzae, suggesting that AVR1-CO39 appeared during the early stage of evolution of P. oryzae. Transformation assays showed that all the cloned homologs tested are functional as an avirulence gene, indicating that selection has maintained their function. Nevertheless, Oryza isolates (isolates virulent on Oryza spp.) in P oryzae were exceptionally noncarriers of AVR1-CO39. All Oryza isolates suffered from one of the two types of known rearrangements at the Avr1-CO39 locus (i.e., G type and J type). These types were congruous to the two major lineages of Oryza isolates from Japan determined by MGR586 and MAGGY. These results indicate that AVR1-CO39 was lost during the early stage of evolution of the Oryza-specific subgroup of P oryzae. Interestingly, its corresponding resistance gene, Pi-CO39(t), is not widely distributed in Oryza spp.
AMER PHYTOPATHOLOGICAL SOC, 2005年11月, MOLECULAR PLANT-MICROBE INTERACTIONS, 18 (11), 1148 - 1160, 英語[査読有り]
研究論文（学術雑誌）
We examined whether PWT4, an avirulence gene of Avena isolates of Magnaporthe oryzae toward wheat, corresponded to Rwt4, a resistance gene identified in wheat cultivar Norin 4, in a one-to-one manner. Twelve wheat cultivars were inoculated with 65X1, an F1 culture with PWT4 derived from a cross between an Avena isolate (Br58) and a Triticum isolate (Br48). Three wheat cultivars (Norin 26, Shin-chunaga, Cheyenne) were resistant and therefore selected as possible carriers of Rwt4. The three cultivars were then inoculated with a population derived from a backcross of 61M2 carrying PWT4 with Br48 carrying pwt4. Segregation analyses revealed that PWT4 operates against the three cultivars. If PWT4 corresponds to Rwt4 in a one-to-one manner, all three cultivars should carry Rwt4. To test if this is the case, the three cultivars were crossed with Chinese Spring (a noncarrier of Rwt4) and Norin 4. When F2 seedlings from Chinese Spring × Norin 26, Chinese Spring × Shin-chunaga, and Chinese Spring × Cheyenne were inoculated with 61M2, resistant and susceptible seedlings segregated in a 3:1 ratio. On the other hand, crosses between the three cultivars and Norin 4 yielded no susceptible F2 seedlings. These results indicate that all three cultivars carry Rwt4. Considering all results, we concluded that PWT4 corresponds to Rwt4 in a one-to-one manner. An inoculation test with Chinese Spring-Cheyenne chromosome substitution lines indicated that Rwt4 is located on chromosome 1D. © The Phytopathological Society of Japan and Springer-Verlag 2005.
2005年10月, Journal of General Plant Pathology, 71 (5), 340 - 344, 英語[査読有り]
研究論文（学術雑誌）
We have developed a pHANNIBAL-like silencing vector, pSilent-1, for ascomycete fungi, which carries a hygromycin resistance cassette and a transcriptional unit for hairpin RNA expression with a spacer of a cutinase gene intron from the rice blast fungus Magnaporthe oryzae. In M. oryzae, a silencing vector with the cutinase intron spacer (147 bp) showed a higher efficiency in silencing of the eGFP gene than did those with a spacer of a GUS gene fragment or a longer intron (850 bp) of a chitin binding protein gene. Application of pSilent-1 to two M. oryzae endogenous genes, MPG1 and polyketide synthase-like gene, resulted in various degrees of silencing of the genes in 70-90% of the resulting transformants. RNA silencing was also induced by a pSilent-1-based vector in Colletotrichum lagenarium at a slightly lower efficiency than in M. oryzae, indicating that this silencing vector should provide a useful reverse genetic tool in ascomycete fungi. (c) 2005 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, 2005年04月, FUNGAL GENETICS AND BIOLOGY, 42 (4), 275 - 283, 英語[査読有り]
研究論文（学術雑誌）
- 2005年, FEBS letters, 579,488-492, 英語
研究論文（学術雑誌）
MAGGY is a Ty3/Gypsy retrotransposon, which was identified in the rice blast fungus Magnaporthe oryzae. Some Ty3/Gypsy retrotransposons, including MAGGY, contain a chromodomain-like module (CLM) in the C-terminus of the integrase domain. We have made a series of MAGGY mutants to examine the role of the CLM in the transposition activity of the element. Introduction of a mutation at different positions in the MAGGY integrase revealed that a loss or alteration of the CLM resulted in a drastic decrease in the transposition activity of the element. Our results indicate that the CLM may confer high transposition activity to the element. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2005年01月, FEBS LETTERS, 579 (2), 488 - 492, 英語[査読有り]
研究論文（学術雑誌）
- 2005年, Fungal Genet. & Biol., 未記入, 英語S. RNA silencing as a tool for exploring gene function in Ascomycete fungi.
研究論文（学術雑誌）
- 2005年, FEBS Lett., 579 5950-5957, 英語
[査読有り]
研究論文（学術雑誌）
- 2005年, J. Gen. Plant. Pathol., 未記入, 英語NO-overproducting transformants of Pseudomonas fluorescens with enhanced biocontrol abiloty against tomato bacterial wilt.
研究論文（学術雑誌）
- 2005年, J. Gen. Plant Pathol., 71 33-38, 英語NO-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol abiloty against tomato bacterial wilt.
[査読有り]
研究論文（学術雑誌）
- 2005年, Molecular Plant-Microbe Interactions, 18 385-396, 385 - 396, 英語Microarray analysis of gene expression profile induced by the endophytic plant growth promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis.
[査読有り]
研究論文（学術雑誌）
- 2005年, Mol. Plant-Microbe Interact., 英語Microarray analysis of gene expression profile induced by the endophytic plant growth promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis.
研究論文（学術雑誌）
We have developed an oat cell-free apoptosis system to investigate the execution mechanisms of plant apoptosis. Cell extracts derived from oat tissues undergoing toxin (victorin)-induced apoptosis caused nuclear collapse and internucleosomal DNA fragmentation in isolated nuclei. Pharmacological studies revealed that cysteine protease, which is E-64-sensitive but insensitive to caspase-specific inhibitors, is a crucial component in the morphological change of isolated nuclei, and that nuclease and the cysteine protease act cooperatively to induce the apoptotic DNA laddering. Interestingly, this finding is contrasted with those in well-studied animal cell-free systems in which an apoptotic endonuclease is solely responsible for the DNA fragmentation. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2004年12月, FEBS LETTERS, 578 (3), 363 - 367, 英語研究論文（学術雑誌）
Dicer is a ribonuclease III-like enzyme playing a key role in the RNA silencing pathway. Genome sequencing projects have demonstrated that eukaryotic genomes vary in the numbers of Dicer-like (DCL) proteins from one ( human) to four ( Arabidopsis). Two DCL genes, MDL-1 and - 2 ( Magnaporthe Dicer-like-1 and - 2) have been identified in the genome of the filamentous fungus Magnaporthe oryzae. Here we show that the knockout of MDL-2 drastically impaired gene silencing of enhanced green fluorescence protein by hairpin RNA and reduced related small interfering RNA ( siRNA) accumulation to nondetectable levels. In contrast, mutating the other DCL, MDL-1, exhibited a gene silencing frequency similar to wild type and accumulated siRNA normally. The silencing-deficient phenotype and loss of siRNA accumulation in the mdl-2 mutant was restored by genetic complementation with the wild-type MDL-2 allele. These results indicate that only MDL-2 is responsible for siRNA production, and no functional redundancy exists between MDL-1 and MDL-2 in the RNA silencing pathway in M. oryzae. Our findings contrast with a recent report in the filamentous fungus Neurospora crassa, where two DCL proteins are redundantly involved in the RNA silencing pathway, but are similar to the results obtained in a more distantly related organism, Drosophila melanogaster, where an individual DCL protein has a distinct role in the siRNA/micro-RNA pathways.
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2004年10月, JOURNAL OF BIOLOGICAL CHEMISTRY, 279 (43), 44467 - 44474, 英語研究論文（学術雑誌）
- Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus
Fungal isolates from gray leaf spot on perennial ryegrass (prg isolates) were characterized by DNA analyses, mating tests, and pathogenicity assays. All of the prg isolates were interfertile with Triticum isolates and clustered into the c top isolate group (CC group) on a dendrogram constructed from rDNA-internal transcribed spacer 2 sequences. Since the CC g-roup corresponded to a newly proposed species, Magnaporthe oryzae, all of the prg isolates were designated M. oryzae. However. DNA finger-printing with MGR586, MGR583. and Pot2 showed that the prg isolates are divided into two distinct populations, i.e., TALF isolates and WK isolates. The TALF isolates were virulent only on Lolium species, whereas the WK isolates were less specific. suggesting that Way leaf spot can he caused not only by Lolium-specific isolates but also by less specific isolates. We designated the TALF isolates as Lolium pathotype. The TALF isolates showed diverse karyotypes in spite of being uniform in DNA fingerprints, Suggesting that they are unstable in genome organization.
AMER PHYTOPATHOLOGICAL SOC, 2004年05月, PHYTOPATHOLOGY, 94 (5), 454 - 462, 英語研究論文（学術雑誌）
- Nitric oxide and reactive oxygen species do not elicit hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oat
Nitric oxide (NO) acts as a signaling molecule in many cellular responses in plants and animals. Oat plants (Avena sativa L.) evoke the hypersensitive response (HR), which shares morphological and biochemical features with mammalian apoptosis, such as DNA laddering and heterochromatin condensation, in response to the avirulent crown rust fungus (Puccinia coronata f. sp. avenae). We examined the role of NO and reactive oxygen species (ROS) in the initiation of hypersensitive cell death, which is induced by direct contact with the pathogen, and apoptotic cell death in the adjacent cells. Cytofluorimetric analysis using the fluorescent NO probe DAF and the H2O2 probe DCF demonstrated that NO and H2O2 were generated simultaneously in primary leaves at an early stage of the defense response. The NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) markedly enhanced H2O2 accumulation detected by 3,3-diaminobenzidine staining and DCF, whereas treatment with the NO donor S-nitroso-N-acetylpenicillantine (SNAP) strongly suppressed it. Superoxide dismutase (SOD) increased NO accumulation, suggesting that endogenous NO may modulate the level of H2O2 by interacting with O-2(-) in the HR lesion. Cytological observation showed that administration of cPTIO, SNAP, or SOD had no effect on elicitation of hypersensitive cell death, but clearly reduced heterochromatin condensation in the nearby cells and DNA laddering. These findings indicate that NO and ROS are not essential mediators for the initiation of hypersensitive cell death. However, NO and O-2(-) but not H2O2 are required for the onset of apoptotic cell death in the adjacent cells, where excess NO may exert its anti-apoptotic function by regulating cellular redox state.
AMER PHYTOPATHOLOGICAL SOC, 2004年03月, MOLECULAR PLANT-MICROBE INTERACTIONS, 17 (3), 245 - 253, 英語[査読有り]
研究論文（学術雑誌）
- Repeat-induced point mutation (RIP) in Magnaporthe grisea: Evidence for the presence of sexual cycle in nature
The RIP (repeat-induced point mutation) process introduces C:G to T:A transitions into duplicated sequences during a specific period in the sexual reproduction and has been reported in only two fungal species, Neurospora crassa and Podospora anserina. To investigate whether a RIP-like process operates in Magnaporthe grisea, the retrotransposon MAGGY and the hygromycin B phosphotransferase gene were introduced into the fungus as multiple transgenes and examined for sequence alterations after asexual growth and sexual crossing. Numerous C:G to T:A transitions were found in the transgenes, preferentially in (A/Tp)Cp(A/T) contexts, in the sexual progenies, suggesting that a RIP-like process functions in M. grisea. We also examined the sequence of another retrotransposon, Pyret, in field isolates of M. grisea. Even though the perfect stage rarely has been observed in M. grisea under field conditions, RIP-like transitions were found in all the field isolates tested and were correlated with the fertility of the isolates in Petri dishes. Analysis of RIP-like transitions in repetitive sequences may provide a tool to estimate the frequency of sexual recombination of M. grisea isolates.
SPRINGER, 2004年, RICE BLAST: INTERACTION WITH RICE AND CONTROL, 57 - 63, 英語[査読有り]
研究論文（国際会議プロシーディングス）
Fungal isolates from gray leaf spot on perennial ryegrass (prg isolates) were characterized by DNA analyses, mating tests, and pathogenicity assays. All of the prg isolates were interfertile with Triticum isolates and clustered into the crop isolate group (CC group) on a dendrogram constructed from rDNA-internal transcribed spacer 2 sequences. Since the CC group corresponded to a newly proposed species, Magnaporthe oryzae, all of the prg isolates were designated M. oryzae. However, DNA fingerprinting with MGR586, MGR583, and Pot2 showed that the prg isolates are divided into two distinct populations, i.e., TALF isolates and WK isolates. The TALF isolates were virulent only on Lolium species, whereas the WK isolates were less specific, suggesting that gray leaf spot can be caused not only by Lolium-specific isolates but also by less specific isolates. We designated the TALF isolates as Lolium pathotype. The TALF isolates showed diverse karyotypes in spite of being uniform in DNA fingerprints, suggesting that they are unstable in genome organization.
American Phytopathological Society, 2004年, Phytopathology, 94 (5), 454 - 462, 英語研究論文（学術雑誌）
Two oat genes encoding hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase (HHT) and S-adenosyl-L-methionine:trans-caffeoyl- CoA 3-O-methyltransferase (CCoAOMT), both of which are possibly involved in the biosynthesis of oat avenanthramide phytoalexins, were cloned and their expression profiles in response to biological stress were studied. Four distinct cDNAs of oat HHT (AsHHT1-4) were isolated with the degenerative polymerase chain reaction method. The enzymatic activity of AsHHT1 expressed in E. coli was found using hydroxyanthranilate and hydroxycinnamoyl-CoAs as cosubstrates. Cloned oat CCoAOMT (AsCCoAOMT) encoded a polypeptide of 130 amino acid residues with 77.7 to 80.8% identities to the CCoAOMT sequences from other plant species. The accumulation of AsHHT1 and AsCCoAOMT transcripts increased concomitantly with phytoalexin accumulation by the treatment of victorin, a specific elicitor in oat lines carrying the Pc-2/Vb gene. Pharmacological approaches indicated the involvement of Ca2+, NO, and protein kinases in the signaling pathways of AsHHT1 and AsCCoAOMT mRNA induction. When oat leaves were inoculated with Puccinia coronata, the mRNA expression of AsHHT1 and AsCCOAOMT increased in both incompatible and compatible interactions but more rapidly in incompatible interaction. Interestingly, however, significant phytoalexin accumulation was observed only in incompatible interaction during the experimental period, suggesting that phytoalexin accumulation may be inhibited in one or more posttranscriptional processes in the compatible interaction.
American Phytopathological Society, 2004年, Molecular Plant-Microbe Interactions, 17 (1), 81 - 89, 英語研究論文（学術雑誌）
- 2004年, Mol. Plant-Microbe Interact., 17,245-253, 英語Nitric oxide and reactive oxygen species are not required for hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oats.
研究論文（学術雑誌）
- Analysis of the involvement of hydroxyanthranilate hydroxycinnamoyltransferase and caffeoyl-CoA 3-O-methyltransferase in phytoalexin biosynthesis in oat
Two oat genes encoding hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase (HHT) and S-adenoSyl-L-methionine:trans-caffeoyl-CoA 3-O-methyltransferase (CCoAOMT), both of which are possibly involved in the biosynthesis of oat avenanthramide phytoalexins, were cloned and their expression profiles in response to biological stress were studied. Four distinct cDNAs of oat HHT (AsHHT1-4) were isolated with the degenerative polymerase chain reaction method. The enzymatic activity of AsHHT1 expressed in E. coli was found using hydroxyanthranilate and hydroxycinnamoyl-CoAs as cosubstrates. Cloned oat CCoAOMT (AsCCoAOMT) encoded a polypeptide of 130 amino acid residues with 77.7 to 80.8% identities to the CCoAOMT sequences from other plant species. The accumulation of AsHHT1 and AsCCoAOMT transcripts increased concomitantly with phytoalexin accumulation by the treatment of victorin, a specific elicitor in oat lines carrying the Pc-2/Vb gene. Pharmacological approaches indicated the involvement of Ca2+, NO, and protein kinases in the signaling pathways of AsHHT1 and AsCCoAOMT mRNA induction. When oat leaves were inoculated with Puccinia coronata, the mRNA expression of AsHHT1 and AsCCOAOMT increased in both incompatible and compatible interactions but more rapidly in incompatible interaction. Interestingly, however, significant phytoalexin accumulation was observed only in incompatible interaction during the experimental period, suggesting that phytoalexin accumulation may be inhibited in one or more posttranscriptional processes in the compatible interaction.
AMER PHYTOPATHOLOGICAL SOC, 2004年01月, MOLECULAR PLANT-MICROBE INTERACTIONS, 17 (1), 81 - 89, 英語研究論文（学術雑誌）
- RNA silencing in the phytopathogenic fungus Magnaporthe oryzae
Systematic analysis of RNA silencing was carried out in the blast fungus Magnaporthe oryzae (formerly Magnaporthe grisea) using the enhanced green fluorescence protein (eGFP) gene as a model. To assess the ability of RNA species to induce RNA silencing in the fungus, plasmid constructs expressing sense, antisense, and hairpin RNAs were introduced into an eGFP-expressing transformant. The fluorescence of eGFP in the transformant was silenced much more efficiently by hairpin RNA of eGFP than by other RNA species. In the silenced transformants, the accumulation of eGFP mRNA was drastically reduced, but no methylation of the promoter or coding region was involved in it. In addition, we found small interfering RNAs (siRNAs) only in the silenced transformants. Interestingly, the siRNAs consisted of RNA molecules with at least three different sizes ranging from 19 to 23 nucleotides, and all of them contained both sense and antisense strands of the eGFP gene. To our knowledge, this is the first demonstration in which different molecular sizes of siRNAs have been found in filamentous fungi. Overall, these results indicate that RNA silencing operates in M. oryzae, which gives us a new tool for genome-wide gene analysis in this fungus.
AMER PHYTOPATHOLOGICAL SOC, 2003年09月, MOLECULAR PLANT-MICROBE INTERACTIONS, 16 (9), 769 - 776, 英語研究論文（学術雑誌）
A 1.2-Mb DNA band from an isolate of Magnaporthe oryzae was detected in a pulsed-field gel. A chromosomal entity corresponding to this band was observed at the mitotic metaphase stage. This minichromosome, carrying many transposable elements and two telomeres, was transmitted to ascosporic F, cultures in a non-Mendelian manner with frequent changes in its size and number. Segregation analysis with RFLP markers indicated that the minichromosome underwent structural rearrangements, such as deletion and duplication, not only during meiosis but also after meiosis. An ectopic sister chromatid recombination may cause the size variation of the minichromosomes.
SPRINGER-VERLAG, 2003年06月, CURRENT GENETICS, 43 (3), 191 - 198, 英語研究論文（学術雑誌）
A 1.2-Mb DNA band from an isolate of Magnaporthe oryzae was detected in a pulsed-field gel. A chromosomal entity corresponding to this band was observed at the mitotic metaphase stage. This minichromosome, carrying many transposable elements and two telomeres, was transmitted to ascosporic F, cultures in a non-Mendelian manner with frequent changes in its size and number. Segregation analysis with RFLP markers indicated that the minichromosome underwent structural rearrangements, such as deletion and duplication, not only during meiosis but also after meiosis. An ectopic sister chromatid recombination may cause the size variation of the minichromosomes.
SPRINGER-VERLAG, 2003年06月, CURRENT GENETICS, 43 (3), 191 - 198, 英語研究論文（学術雑誌）
Host species specificity of Magnaporthe grisea toward foxtail millet was analyzed using F, cultures derived from a cross between a Triticum isolate (pathogenic on wheat) and a Setaria isolate (pathogenic on foxtail millet). On foxtail millet cvs. Beni-awa and Oke-awa, avirulent and virulent cultures segregated in a 1:1 ratio, suggesting that a single locus is involved in the specificity, This locus was designated as Pfm1. On cv. Ki-awa, two loci were involved and one of them was Pfm1. The other locus was designated as Pfm2. Interestingly, Pfm1 was not involved in the pathogenic specificity on cv. Kariwano-zairai. These results suggest that there is no "master gene" that determines the pathogenic specificity on all foxtail millet cultivars and that the species specificity of M. grisea toward foxtail millet is governed by cultivar-dependent genetic mechanisms that are similar to gene-for-gene interactions controlling race-cultivar specificity.
AMER PHYTOPATHOLOGICAL SOC, 2003年01月, PHYTOPATHOLOGY, 93 (1), 42 - 45, 英語[査読有り]
研究論文（学術雑誌）
- 2003年, Mol. Plant-MicrobeInteract., 未記入, 英語Nitric oxide and reactive oxygen species are not required for hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oats.
研究論文（学術雑誌）
An isolate of Magnaporthe grisea was collected from a blast lesion on oat in Brazil. Sequence analysis of the rDNA-ITS-2 region and DNA fingerprinting with repetitive elements revealed that the Avena isolate belongs to the "crop isolate group" and is similar to Triticum isolates. At high temperature (28degreesC), the Avena isolate caused severe disease symptoms on primary leaves of oat and wheat. When the temperature was decreased to 20degreesC, wheat leaves expressed resistance to the Avena isolate. Cytologically, this temperature-dependent resistance was associated with an increase in the incidences of papilla formation and a hypersensitive reaction. Pathogenicity tests with various plant species at 20degreesC revealed that the Avena isolate is exclusively parasitic on oat. To elucidate genetic mechanisms of this species-specific parasitism, the Avena isolate was crossed with a Triticum isolate and resulting F-1 progenies were subjected to pathogenicity tests on oat seedlings. In the F-1 population, avirulent and virulent cultures segregated in a 1:1 ratio, suggesting that the specific parasitism on oat is controlled by a single gene. This locus was designated as Pat1.
NATL RESEARCH COUNCIL CANADA, 2002年10月, CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 80 (10), 1088 - 1095, 英語[査読有り]
研究論文（学術雑誌）
- Apoptotic cell death is a common response to pathogen attack in oats
We have examined the characteristics of cell death induced by pathogen infection in oats with respect to following hallmark apoptotic features: DNA laddering, chromatin condensation, and electron microscopic-terminal deoxynucleotidyl transferase-mediated UTP end labeling positive response. A wide range of plant pathogens representing different levels of parasitism in susceptible and resistant interactions were used for the inocula, which include (i) an obligate parasite, Puccinia coronata f. sp. avenue (the crown rust fungus); (ii) a facultative biotroph parasite, Magnaporthe grisea (the blast fungus); (iii) pathogenic bacteria, Pseudomonas syringae pv. atro-purpurea and P syringae pv. coronafaciens (the halo or stripe blights of oats); and (iv) Ryegrass mottle virus. Surprisingly, any of the pathogens used induced most of the apoptotic features in oat cells at and around the infection sites, indicating that apoptotic cell death is a common phenomenon in oats during pathogen attack. The localization and the timing of apoptotic cell death during a course of infection were, however, quite different depending on the interactions (compatible or incompatible) and the pathogens (fungi, bacteria, or viruses). Possible roles of apoptotic cell death in the susceptible and resistant interactions are discussed.
AMER PHYTOPATHOLOGICAL SOC, 2002年10月, MOLECULAR PLANT-MICROBE INTERACTIONS, 15 (10), 1000 - 1007, 英語[査読有り]
研究論文（学術雑誌）
- Repeat-induced point mutation (RIP) in Magnaporthe grisea: implications for its sexual cycle in the natural field context
Repeat-induced point mutation (RIP) is a process that detects DNA duplications and peppers their sequences with C:G to T:A transitions in the sexual phase of the life cycle. So far, this unique mechanism has been identified as a currently active process in only two fungal species, Neurospora crassa and Podospora anserina. To determine whether a RIP-like process operates in the plant pathogenic fungus Magnaporthe grisea, the retrotransposon MAGGY and the hygromycin B phosphotransferase gene were introduced into the fungus as multiple transgenes and examined for sequence alterations after a cross. Frequent C:G to T:A transitions in the transgenes were found in the descendants, preferentially in (A/Tp)Cp(A/T)contexts, suggesting that a process similar to RIP functions in M. grisea. We also examined the sequence of another retrotransposon Pyret in six field isolates of M. grisea. Even though no perfect stage has been known in M. grisea under field conditions to date, RIP-like transitions were found in all the field isolates tested. Interestingly, the frequency of the transitions mostly correlated with the fertility of the isolates examined under laboratory conditions. These results imply that the sexual cycle of this fungus exists or existed in the natural field context.
WILEY-BLACKWELL, 2002年09月, MOLECULAR MICROBIOLOGY, 45 (5), 1355 - 1364, 英語[査読有り]
研究論文（学術雑誌）
Apoptotic cell response in oats is induced by victorin, a host-selective toxin secreted by Cochliobolus victoriae and thought to exert toxicity by inhibiting mitochondrial glycine decarboxylase (GDC) in Pc-2/Vb oats. We examined the role of mitochondria, especially the organelle-derived production of reactive oxygen species (ROS), in the induction of apoptotic cell death. Cytofluorimetric analysis showed that victorin caused mitochondrial AT,, breakdown and mitochondrial oxidative burst. Ultrastructural analysis using a cytochemical assay based on the reaction of H2O2 with CeCl3 detected H2O2 eruption at permeability transition pore-like sites on the mitochondrial membrane in oat cells treated with victorin. ROS generation preceded the apoptotic cell responses seen in chromatin condensation and DNA laddering. Both aminoacetonitrile (a specific GDC inhibitor) and antimycin A (a mitochondrial complex III inhibitor) also induced mitochondrial H2O2 eruption, and led to the apoptotic response in oat cells. ROS scavengers such as N-acetyl-L-cysteine and catalase suppressed the mitochondrial oxidative burst and delayed chromatin condensation and DNA laddering in the victorin- or antimycin A-treated leaves. These findings indicate possible involvement of mitochondria, especially mitochondrial-derived ROS generation, as an important regulator in controlling apoptotic cell death in oats.
BLACKWELL PUBLISHING LTD, 2002年06月, PLANT JOURNAL, 30 (5), 567 - 579, 英語[査読有り]
研究論文（学術雑誌）
- 神戸大学, 2002年02月25日, 神戸大学農学部学術報告, 26, 1 - 7, 英語
- Analysis of the structure of the AVR1-CO39 avirulence locus in virulent rice-infecting isolates of Magnaporthe grisea
The AVR1-CO39 gene that came from a Magnaporthe grisea isolate from weeping lovegrass controls avirulence on the rice cultivar CO39. AVR1-CO39 was not present in the genome of the rice-infecting M. grisea isolate Guy11 from French Guyana, suggesting that the gene had been deleted. Molecular analysis of the deletion breakpoints in the AVR1-CO39 locus revealed the presence of a truncated copy of a previously unknown retrotransposon at the lefthand border. At the right-hand border was a truncated copy of another repetitive element that is present at multiple locations in the genome of Guy11. The structures of avr1-CO39 loci were further examined in 45 rice-infecting isolates collected in Brazil, China, Japan, India, Indonesia, Mali, and the Philippines. Most isolates showed no hybridization signal with the AVR1-CO39 probe and had the same locus structure as Guy11. Some isolates from Japan showed a signal with the AVR1-CO39 probe, but the region specifying avirulence activity was rearranged. These findings suggest that widespread virulence to 'CO39' among rice-infecting M. grisea isolates is due to ancestral rearrangements at the AVR1-CO39 locus that may have occurred early in the evolution of pathogenicity to rice.
AMER PHYTOPATHOLOGICAL SOC, 2002年01月, MOLECULAR PLANT-MICROBE INTERACTIONS, 15 (1), 6 - 16, 英語[査読有り]
研究論文（学術雑誌）
Transcriptional activity of the retrotransposon OARE-1 was monitored in oat leaves infected with pathogenic fungi. The transcription of OARE-1 was upregulated in both incompatible and compatible interactions between an oat cultivar and Magnaporthe grisea isolates but more extensively in the compatible. OARE-1 was also strongly activated in a Pc2/Vb-carrying oat line inoculated with Helminthosporium victoriae. The upregulation in the Pc2/Vb-carrying oat line was reproduced by treatment with victorin C, the major compound of the host-specific toxin produced by the fungus. These results suggest that OARE-1 is responsive to various signals or stresses associated with compatibility and incompatibility.
Phytopathological Society of Japan, 2002年, Journal of general plant pathology : JGPP, 68 (1), 8 - 14, 英語[査読有り]
- OARE-1, a Ty1-copia retrotransposon in oat activated by abiotic and biotic stresses
Transcriptionally active Ty1-copia LTR-retrotransposons were found in oat using RT-PCR for amplifying the reverse transcriptase domain. Sequence analysis of the RT-PCR clones suggested that oat LTR-retrotransposons consist of at least seven groups, which were tentatively designated as Oatrt1 to Oatrt7. A full length copy of Oatrt1 was isolated from an oat genomic library, and was designated OARE-1. OARE-1 was 8,665 bp long and a member of the BARE-1 subgroup. The oat genome carried it in multiple copies (at least 10,000 copies/a hexaploid genome). The expression of OARE-1 was intensively induced by wounding, UV light, jasmonic acid and salicylic acid, and its pattern was very similar to that of the PAL (phenylalanin ammonia lyase) gene. Furthermore, OARE-1 was highly activated by infection with an incompatible race of the crown rust fungus, Puccinia coronata. These results suggest that OARE-1 is highly sensitive to various abiotic and biotic stimuli leading to plant defense responses.
OXFORD UNIV PRESS, 2001年12月, PLANT AND CELL PHYSIOLOGY, 42 (12), 1345 - 1354, 英語[査読有り]
研究論文（学術雑誌）
- Heat shock, copper sulfate and oxidative stress activate the retrotransposon MAGGY resident in the plant pathogenic fungus Magnaporthe grisea
MAGGY is a gypsy-like retrotransposon isolated from the plant pathogenic fungus Magnaporthe grisea. The ability of various stresses to activate MAGGY was tested in the original and in a heterologous host (Colletotrichum lagenarium), using beta -glucuronidase (GUS) as a reporter. The MAGGY promoter was activated in M. grisea by either heat shock, copper sulfate, or oxidative stress, but not by the antifungal substance p-coumaric acid. Transcriptional up-regulation of MAGGY RNA was also observed following heat shock and oxidative stress. The MAGGY promoter remained responsive to the above-mentioned stresses when transformed into a M. grisea isolate that had no endogenous MAGGY elements. In C. lagenarium, however, the MAGGY promoter showed only basal expression of GUS and no further up-regulation was induced by any of the stress treatments, suggesting that the stress-responding cis-elements) in the MAGGY promoter is not functional in a wider range of fungi. The relationship between the activation of MAGGY by stress and phenotypic diversification in M. grisea, including variations in pathogenicity, is discussed.
SPRINGER-VERLAG, 2001年10月, MOLECULAR GENETICS AND GENOMICS, 266 (2), 318 - 325, 英語[査読有り]
研究論文（学術雑誌）
- Novel evidence for apoptotic cell response and differential signals in chromatin condensation and DNA cleavage in victorin-treated oats
Histological and cytological evidence of where and when apoptotic cells occur in Pc-2/Vb oat cells treated with victorin was obtained by observing DNA strand breaks at both light (LM) and electron microscope (EM) levels using TUNEL techniques. DNA from leaf segments that had been floated on victorin solution with the abaxial epidermis removed showed typical ladders on agarose gels. Nuclear chromatin condensation, followed by cell collapse, started in the mesophyll cells closest to the victorin solution. LM-TUNEL was positive in the non-collapsed cells but not in the collapsed cells in the treated leaves. However, the EM-TUNEL assay was positive in the nuclei of the non-collapsed as well as the collapsed cells where nuclear fragments dispersed into the cytoplasm, and the immunogold density was much higher than that in the cells killed by a high concentration of H2O2, suggesting that the victorin-treated collapsed cells are in the last stage of apoptotic cell death. The immunogold labelling in the victorin-treated non-collapsed cells was restricted to condensed heterochromatin, indicating that chromatin condensation is associated with DNA cleavage. Pharmacological studies indicated that proteases and nucleases may play a role in the apoptotic response. However, the EM-TUNEL assay indicated that EGTA co-incubated with victorin blocked DNA cleavage, but failed to prevent chromatin condensation. Moreover, protein kinases were involved in chromatin condensation, but did not affect DNA digestion, suggesting that chromatin condensation and DNA cleavage are differentially regulated in the death process in oats.
BLACKWELL SCIENCE LTD, 2001年10月, PLANT JOURNAL, 28 (1), 13 - 26, 英語[査読有り]
研究論文（学術雑誌）
- Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains
A novel Ty3/Gypsy retrotransposon, named Pyret, was identified in the plant pathogenic fungus Magnaporthe grisea (anamorph Pyricularia oryzae). Pyret-related elements were distributed in a wide range of Pyricularia isolates from various gramineous plants. The Pyret element is 7250 bp in length with a 475 bp LTR and one conceptual ORF. The ORF contains seven nonsense mutations in the reading frame, indicating that the Pyret clone is lightly degenerate. Comparative domain analysis among retroelements revealed that Pyret exhibits an extra domain (WCCH domain) beyond the basic components of LTR retrotransposons. The WCCH domain consists of similar to 300 amino acids and is located downstream of the nucleocapsid domain. The WCCH domain is so named because it contains two repeats of a characteristic amino acid sequence, W-X-2-C-X-4-C-X-2-H-X-3-K. A WCCH motif-like sequence is found in the precoat protein of some geminiviruses, viral RNA-dependent RNA polymerase and also in an Arabidopsis protein of unknown function. Interestingly, detailed sequence analysis of the gag protein revealed that Pyret, as well as some other chromodomain-containing LTR retrotransposons, displays significant sequence homology with members of the gammaretroviruses (MLV-related retroviruses) in the capsid and nucleocapsid domains. This suggests that chromodomain-containing LTR retrotransposons and gammaretroviruses may share a common ancestor with the gag protein.
OXFORD UNIV PRESS, 2001年10月, NUCLEIC ACIDS RESEARCH, 29 (20), 4106 - 4113, 英語[査読有り]
研究論文（学術雑誌）
- Induction and signaling of an apoptotic response typified by DNA laddering in the defense response of oats to infection and elicitors
Cells in the primary leaves of oats displayed internucleosomal DNA cleavage in response to incompatible crown rust infection. DNA laddering also was evident in leaves treated with calcium ionophore A23187, nonspecific elicitors such as chitin and chitosan oligomers, and victorin, which functions as a specific elicitor in Pc-2/Vb containing oat leaves. The nuclei in a victorin-treated susceptible oat line were positive for the TUNEL assay. These elicitors clearly induced a 28-kDa nuclease (p28) in addition to three constitutive nucleases of 33, 24, and 22 kDa, Activation of p28 preceded the appearance of DNA laddering and possibly was mediated by de novo synthesis and/or cysteine protease activity. Pharmacological studies showed that the induction of DNA laddering was associated with oxidative stress, Ca2+ influx, and serine and cysteine proteases, Protein kinase and calmodulin activities did not seem to be involved in the induction of DNA laddering by victorin, whereas kinase-mediated signals were involved in DNA laddering induced by A23187. Protein kinase, calmodulin, G-protein activities, and Ca2+ influx, however, are involved in phytoalexin production. Our results imply that p28 is a possible nuclease candidate responsible for the induction of DNA laddering. The results also demonstrated that the mediators involved in the induction of apoptosis depended on the type of stimuli, whereas p28 and serine and cysteine proteases commonly are associated with each elicitor-induced apoptosis.
AMER PHYTOPATHOLOGICAL SOC, 2001年04月, MOLECULAR PLANT-MICROBE INTERACTIONS, 14 (4), 477 - 486, 英語[査読有り]
研究論文（学術雑誌）
- Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea
We: have introduced the LTR-retrotransposon MAGGY into a naive genome of Magnaporthe grisea and estimated the copy number of MAGGY in a cell by serial isolation of fungal protoplasts at certain time intervals. The number of MAGGY elements rapidly-increased for a short period following introduction. However, it did not increase geometrically and reached equilibrium at 20-30 copies per genome, indicating that MAGGY was repressed or silenced during proliferation, De novo methylation of MAGGY occurred immediately following invasion into the genome but the degree of methylation was constant and did not correlate with the repression of MABGGY. 5-Azacytidine treatment demethylated and transcriptionally activated the MAGGY element in regenerants but did not affect transpositional frequency, suggesting that post-transcriptional suppression, not methylation, is the main force that represses MAGGY proliferation in M.grisea, Support for this conclusion was also obtained by examining the methylation status of MAGGY sequences in field isolates of M.grisea with active or inactive MAGGY elements. Methylation of the MAGGY sequences was detected in some isolates but not in others, However, the methylation status did not correlate with the copy numbers and activity of the elements.
OXFORD UNIV PRESS, 2001年03月, NUCLEIC ACIDS RESEARCH, 29 (6), 1278 - 1284, 英語[査読有り]
研究論文（学術雑誌）
- AMER PHYTOPATHOLOGICAL SOC, 2001年, DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS, 220 - 228, 英語Apoptotic response in defence of oats to infections and elicitors
[査読有り]
研究論文（国際会議プロシーディングス）
- Comparative analyses of the distribution of various transposable elements in Pyricularia and their activity during and after the sexual cycle
We examined the distribution and activity of six transposable elements found in the blast fungus, Pyricularia spp. Sixty-eight isolates from various gramineous plants were used for the survey, and the elements were plotted on a dendrogram constructed on the basis of their rDNA-ITS2 sequences. MGR586 and Pot2 (Class II elements), Mg-SINE (SINE-like element) and MGR583 (LINE-like retrotransposon) were widely distributed among the Pyricularia isolates, suggesting that they are old elements which arose in, or invaded, the Pyricularia population at very early stages in its evolution. By contrast, the distribution of the LTR-retrotransposons MAGCY and Grasshopper was limited or sporadic, suggesting that they are relatively new elements which recently invaded the Pyricularia population by means of horizontal transfer events. The activity of these elements was evaluated by Southern analysis in progenies derived from a cross between a Setaria isolate and a Triticum isolate. Many new MAGGY signals were observed, which were absent in the parental isolates, at various stages of the sexual cycle and following vegetative growth. In contrast, the other elements yielded few, if any, such signals. Analysis of the sequences flanking the new MAGGY insertions revealed that they were each associated with a 5-bp target-site duplication at both ends of the insertion. These data suggested that MAGGY was the most active of the elements tested for transposition in Pyricularia.
SPRINGER-VERLAG, 2001年01月, MOLECULAR AND GENERAL GENETICS, 264 (5), 565 - 577, 英語[査読有り]
研究論文（学術雑誌）
A genetic cross was performed between a Setaria isolate (pathogenic on foxtail millet) and a Triticum isolate (pathogenic on wheat) of Magnaporthe grisea to elucidate genetic mechanisms of its specific parasitism toward wheat. A total of 80 F(1) progenies were obtained from 10 mature asci containing 8 ascospores. Lesions on wheat leaves produced by the Fl progenies were classified into four types, which segregated in a 1:1:1:1 ratio. This result suggested that the pathogenicity of the F(1) population on wheat was controlled by two genes located at different loci. This idea was supported by backcross analyses. We designated these loci as Pwt1 and Pwt2. Cytological analyses revealed that Pwt1 and Pwt2 were mainly associated with the hypersensitive reaction and papilla formation, respectively.
AMER PHYTOPATHOLOGICAL SOC, 2000年10月, PHYTOPATHOLOGY, 90 (10), 1060 - 1067, 英語[査読有り]
研究論文（学術雑誌）
- Retrotransposition of MAGGY in Pyricularia spp.
MAGGY is a gypsy-like LTR retrotransposon found in,rice isolates of the blast fungus, Pyricularia grisea (teleomorph, Magnaporthe grisea). In this study, we examined transposition of MAGGY in Pyricularia isolates that did not originally possess a MAGGY element. Genomic Southern analysis of MAGGY-transformants suggested that transposition of MAGGY occurred in all isolates tested. In contrast, no transposition was observed in all transformants with a modified MAGGY containing a 513-bp deletion in the reverse transcriptase domain. When a MAGGY derivative carrying an artificial intron was introduced into the wheat isolate of P. grisea, loss of the intron was observed. These results showed that MAGGY can undergo autonomous RNA-mediated transposition in all Pyricularia isolates. This supports the hypothesis that MAGGY was acquired horizontally after the differentiation of MAGGY-carriers and its non-carriers.
SPRINGER, 2000年, ADVANCES IN RICE BLAST RESEARCH, 15, 323 - 329, 英語[査読有り]
研究論文（国際会議プロシーディングス）
- Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi
MAGGY is a gypsy like LTR retrotransposon isolated from the blast fungus Pyricularia grisea (teleomorph, magnaporthe grisea). We examined transposition of MAGGY in three P. grisea isolates (wheat, finger millet, and crabgrass pathogen), which did not originally possess a MAGGY element, and in two heterologous species of filamentous fungi, Colletotrichum lagenarium and P. zingiberi. Genomic Southern analysis of MAGGY transformants suggested that transposition of MAGGY occurred in all filamentous fungi tested. In contrast, no transposition was observed in any transformants with a modified MAGGY containing a 513-bp deletion in the reverse transcriptase domain. When a MAGGY derivative carrying an artificial intron was introduced into the wheat isolate of P. grisea and C. lagenarium, loss of the intron was observed. These results showed that MAGGY can undergo autonomous RNA-mediated transposition in heterologous filamentous fungi. The frequency of transposition differed among fungal species. MAGGY transposed actively in the wheat isolate of P. grisea and P. zingiberi, but transposition in C. lagenarium appeared to be rare. This is the first report that demonstrates active transposition of a fungal transposable element in heterologous hosts. Possible usage of MAGGY as a genetic tagging tool in filamentous fungi is discussed.
GENETICS, 1999年10月, GENETICS, 153 (2), 693 - 703, 英語[査読有り]
研究論文（学術雑誌）
- Degenerate MAGGY elements in a subgroup of Pyricularia grisea: a possible example of successful capture of a genetic invader by a fungal genome
The LTR-retrotransposon MAGGY is found sporadically in isolates of Pyricularia grisea (Magnaporthe grisea). Based on a dendrogram constructed by RFLP analysis of rDNA, isolates that carry MAGGY elements were classified into a single cluster that comprised four rDNA types. However, in a few members of this cluster, exemplified by isolates from common millet (Panicum miliaceum), the MAGGY element has distinct features. Southern analysis suggested that these isolates possessed a single copy of a MAGGY-related sequence whose restriction map differed from that of MAGGY itself. Sequence analysis revealed that the MAGGY-related sequence was a degenerate form of MAGGY, characterized by numerous C:G to T:A transitions, which have often been reported to result from RIP (Repeat-induced point mutation) or RIP-like processes. However, the favored target site for C:G to T:A transitions in this fungus, determined by examining a total of 501 sites, was (A/T)pCp(A/T), which differs from that for the RIP process originally reported in Neurospora (CpA), and from that reported in Aspergillus (CpC). The fact that certain members of the cluster of MAGGY carriers retain a single copy of a degenerate MAGGY element implies that the ancestor of these isolates successfully "captured" the invading MAGGY element.
SPRINGER VERLAG, 1999年07月, MOLECULAR AND GENERAL GENETICS, 261 (6), 958 - 966, 英語[査読有り]
研究論文（学術雑誌）
Cauliflower mosaic virus (CaMV) open reading frame (ORF) III encodes a 15 kDa protein; the function of which is as yet unknown, This protein has non-sequence-specific DNA binding activity and is associated with viral particles, suggesting that the ORF III product (PS) is involved in the folding of CaMV DNA during encapsidation, In this study, we demonstrated that P3 forms a tetramer in CaMV-infected plants. A P3-related protein with an apparent molecular weight of 60 kDa was detected by Western blotting analysis using anti-P3 antiserum under non-reducing conditions, while only 15 kDa P3 was detected under reducing conditions. Analysis of P3 using viable mutants with a 27-bp insertion in either ORF III or TV revealed that the 60 kDa protein was a tetramer of P3, The P3 tetramer co-sedimented with viral coat protein in multiple fractions on sucrose gradient centrifugation, suggesting that P3 tetramer binds to mature and immature virions. These results strongly suggested that CaMV P3 forms a tetramer in planta and that disulfide bonds are involved in its formation and/or stabilization. The finding of P3 tetramer in planta suggested that viral DNA. would be folded compactly by the interaction with multiple P3 molecules, which would form tetramers, while being packaged into the capsid shell.
CENTER ACADEMIC PUBL JAPAN, 1999年, MICROBIOLOGY AND IMMUNOLOGY, 43 (8), 773 - 780, 英語[査読有り]
研究論文（学術雑誌）
Studies have indicated that cauliflower mosaic virus (CaMV) gene expression is mediated by the translation of polycistronic 35S pregenomic RNA, but the involvement of some minor subgenomic RNA species is also suspected. We examined the involvement of the 35S promoter in the expression of CaMV open reading frames (ORFs) I and TV using both 35S RNA-driven and promoter-less ORF I- and ORF IV-beta-glucuronidase (GUS) fusion constructs. In addition to the 35S promoter-dependent expression of both ORF I- and IV-GUS fusions, rye detected the 35S promoter-independent expression of both fusion genes via subgenomic mRNAs, which were detected by Northern blotting in the protoplasts transfected with the 35S promoter-driven constructs as well as in those transfected with the promoter-less constructs. These results suggest the involvement of subgenomic RNAs in the expression of CaMV ORFs I and IV: and the operation of a dual strategy in the expression of two viral genes.
CENTER ACADEMIC PUBL JAPAN, 1998年, MICROBIOLOGY AND IMMUNOLOGY, 42 (4), 329 - 334, 英語[査読有り]
研究論文（学術雑誌）
The expression of cauliflower mosaic virus (CaMV) genes was studied in a turnip protoplast system. Six CaMV-encoded gene products were detected in infected turnip protoplasts by means of Western blotting. The infected turnip protoplasts showed different patterns of protein accumulation; e.g. an open reading frame (ORF) I-encoded movement protein, an ORF V-encoded reverse transcriptase and an ORF VI-encoded posttranscriptional transactivator representing the early accumulated proteins, an ORF II-encoded aphid transmission factor and an ORF IV-encoded coat protein the late accumulated proteins and an ORF III-encoded DNA binding protein the intermediate protein. The results suggest that the expression of CaMV genes is differentially regulated.
CENTER ACADEMIC PUBL JAPAN, 1998年, MICROBIOLOGY AND IMMUNOLOGY, 42 (1), 65 - 69, 英語[査読有り]
研究論文（学術雑誌）
Cauliflower mosaic virus (CaMV) open reading frame (ORF) VI product (P6) has been shown to be the major constituent of viral inclusion body, to function as a post-transcriptional transactivator, and to be essential for infectivity on whole plants. Although these findings suggest that P6 has an important role in viral multiplication, it is unknown whether P6 is required for viral multiplication in a single cell. To address this question, we transfected turnip protoplasts with an ORF VI frame-shift (4 bp deletion) mutant (pCaFS6) of an infectious CaMV DNA clone (pCa122). The mutant was uninfectious. Co-transfection of plasmids expressing P6 complemented the mutant. Overexpression of P6 elevated the infection rate in co-transfection experiments with either pCa122 or pCaFS6. This would have been achieved by elevating the level of pregenomic 35S RNA, a putative polycistronic mRNA for ORFs I, II, III, IV and V, and by enhancing the accumulation of these five viral gene products. When CaMV ORFs I, II, III, TV and V were expressed from monocistronic constructs in which each of the ORFs was placed just downstream of the 35S promoter, the accumulation of ORF III, IV and V products depended an the co-expression of P6. The accumulation of ORF I and II products was not detected, even in the presence of P6. These results suggest that P6 is involved in the stabilization of other viral gene products as well as in the activation of viral gene expression, and thus, is a prerequisite for CaMV multiplication.
CENTER ACADEMIC PUBL JAPAN, 1998年, MICROBIOLOGY AND IMMUNOLOGY, 42 (5), 377 - 386, 英語[査読有り]
研究論文（学術雑誌）
- Genetical and physiological studies on the species-specific parasitism of Magnaporthe grisea in gramineous plants with a special reference to a cross between the Triticum isolate and the Setaria isolate
Pyricularia grisea, the blast fungus of many gramineous plants, includes several host-specific subgroups, A phenogram constructed from rDNA-RFLP data showed that subgroups pathogenic on crops (i.e., rice, foxtail millet, common millet; finger millet, and wheal) and the subgroup pathogenic on crabgrass were classified into different clusters and that this fungeal species has a high diversity. The distribution of MAGGY on the phenogram suggested that this retrotransposon was acquired by a common ancestor of four rDNA types including Oryza isolates (pathogenic oil rice) and Setaria isolates (pathogenic on foxtail millet). To elucidate mechanisms of the species-specific parasitism in gramineous crops classified as a subgroup; we crossed a Setaria isolate with a Triticum isolate (pathogenic on wheat and with no MAGGY), and produced an F-1 population which (i) showed segregation in pathogenicity, phytotoxic compounds, and MAGGY copies, (ii) included mutants in mycelial color: appressorium formation, pathogenicity, and phytotoxic compounds. and (iii) contained many transposed copies of MAGGY. The usefulness of this population for analyses of the species-specific parasitism was discussed.
SPRINGER, 1998年, MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES, 13, 211 - 221, 英語[査読有り]
研究論文（国際会議プロシーディングス）
- Accumulation of diferulic acid during the hypersensitive response of oat leaves to Puccinia coronata f sp avenae and its role in the resistance of oat tissues to cell wall degrading enzymes
The relationship between diferulic acid formation and locally induced resistance was investigated in the interactions of two physiological races of Puccinia coronata f.sp. avenae with Shokan 1 oat. Diferulic acid was detected in the hemicellulosic fraction extracted with oxalic acid from the cell wall. The amount of ferulic acid bound in the cell wall matrix polysaccharides did not vary in uninoculated and inoculated primary leaves of oars during the rime course of the experiment. In contrast, the formation of diferulic acid bound to wall polysaccharides became significantly higher in the incompatible than the compatible interaction at approx. 36 h after inoculation. The timing of increased formation of diferulic acid was correlated with the accumulation of avenalumins and inhibition of protoplast liberation from infected primary leaves. Activities of soluble, and of ionically- and covalently-bound cell wall peroxidases from uninoculated and inoculated leaves were measured. Ionically-bound cell wall peroxidase activity increased in the incompatible interactions at about 36 h after inoculation. This was the same time that increased levels of diferulic acids were observed. This may indicate that the cross-linking of matrix polysaccharides by ferulic acids bound in cell walls is caused by a coupling reaction initiated by ionically-bound cell wall peroxidases. It is postulated that the diferulic acid formation occurs in oat cell walls at the infection sites in incompatible interactions and may contribute to locally induced resistance in oats by providing a barrier to pathogen ingress. (C) 1996 Academic Press Limited.
ACADEMIC PRESS LTD, 1996年04月, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 48 (4), 245 - 255, 英語[査読有り]
研究論文（学術雑誌）
- AMER PHYTOPATHOLOGICAL SOC, 1996年, MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION, 221 - 231, 英語Expression of specific resistance in crown rust oat interaction
[査読有り]
研究論文（国際会議プロシーディングス）
ブロムモザイクウイルス(BMV) ATCC 66株の感染性 in vitro RNAを転写できるcDNAクローン(KU 1株) の全塩基配列を決定し, BMVロシア株と比較した。BMV RNA 1, 2, 3それぞれにおけるKU 1株とロシア株の間での相同性は97%以上で, transitionタイプの置換がtransversionタイプの置換よりも多かった。Sindbis様植物RNA ウイルスに保存されている3つの領域でのアミノ酸変異のほとんどは類似アミノ酸への変換であった。RNA1と3の5'端非翻訳領域の塩基配列は両株間で完全に同じであったが, RNA 2では7つの塩基が異なっていた。いずれのRNA の3'端の塩基配列の変異も想定される2次構造に影響を与えないものであった。KU 1株, ATCC 66株ともに外被タンパク質遺伝子の5'端非翻訳領域において2つの隣接したアデニン塩基が欠失していた。RNA 3のシスロトン間領域に存在するオリゴ(A)配列のアデニンの数はATCC 66株では一定ではなく, KU 1株のRNA 3のcDNAでグアニン塩基が1個含まれていたのに対して, ATCC 66株のRNA 3では2個以上のグアニン塩基が認められた。
日本植物病理学会, 1994年08月, 日本植物病理學會報, 60 (4), 454 - 462, 英語[査読有り]
研究論文（学術雑誌）
カリフラワーモザイクウイルス(CaMV), CM1841系統は, 昆虫媒介性に関与するウイル遺伝子(ORF II)にアミノ酸置換がある非昆虫媒介性のCaMV分離株として知られてきた。CM 1841感染葉では, ORF II産物(p18)蓄積量が昆虫媒介性のCaMVと比較して少ないが, 病徴発現初期には昆虫媒介性のCaMV系統の25〜50%程度のp18が蓄積している (Nakayashiki et al. J. Gen. Virol.74 : 2469-2472, 1993)。そこで病徴発現初期におけるCM 1841系統の昆虫媒介性を検討した。通常のアブラムシ頭数(10〜20)による検定では媒介は認められなかったが, 多頭数(200〜300)のアブラムシを用いて検討を行ったところ, これまで非昆虫媒介性と考えられていたCM 1841系統でアブラムシによる媒介が初めて確認された。
日本植物病理学会, 1994年, Annals of the Phytopathological Society of Japan, 60 (4), 496 - 500, 英語[査読有り]
The synthesis and accumulation of aphid transmission factor protein (p18) in cauliflower mosaic virus (CaMV)-infected turnip protoplasts were examined in time course and pulse-labelling experiments, comparing an aphid-non-transmissible isolate (CM1841) with an in vitro recombinant aphid-transmissible CaMV (CMBX) generated from the CM1841 isolate. There was little difference in the synthesis and accumulation of p18 between CM1841- and CMBX-infected protoplasts. When the accumulation of p18 in infected leaves was monitored from 3 to 28 days post-symptom emergence (p.e.) by Western blotting, the amount of p18 accumulated in CM 1 84 1 -infected leaves continuously decreased from 3 days p.e. throughout the experimental period, whereas the amount of p18 in CMBX-infected leaves was lowest at 3 days p.e. and increased thereafter. These results suggested that CM 1 841 differed from CMBX not in the synthesis of p18 but in the stability of p18 in infected leaves.
SOC GENERAL MICROBIOLOGY, 1993年11月, JOURNAL OF GENERAL VIROLOGY, 74, 2469 - 2472, 英語[査読有り]
MISC
- 2022年, 日本植物病理学会大会プログラム・講演要旨予稿集, 2022イネ根へのいもち病菌の感染において品種特異的抵抗性は機能しない
- 2022年, 日本植物病理学会報, 88 (1)いもち病菌の病原性発現過程における糖代謝と脂質代謝のクロストーク
- 2022年, 日本植物病理学会報, 88 (1)いもち病菌の根への感染機構の解明(1)各種病原型菌株を接種したイネ科植物の反応特性
- 2021年, 日本植物病理学会報, 87 (1)コムギいもち病菌における定量PCR法及びLAMP法による検出用プライマーの検討
- 2021年, 日本植物病理学会大会プログラム・講演要旨予稿集, 2021コムギいもち病菌における新規特異的検出用qPCR/LAMPプライマーの設計
- 2019年05月, Journal of General Plant Pathology, 85 (3), 163 - 173, 英語Cell biology in phytopathogenic fungi during host infection: commonalities and differences
[査読有り]
記事・総説・解説・論説等（学術雑誌）
- GENETICS SOC JAPAN, 2016年12月, GENES & GENETIC SYSTEMS, 91 (6), 360 - 360, 英語An Argonaute gene in the fungus Magnaporthe oryzae functions as a negative regulator of RNA silencing
研究発表ペーパー・要旨（国際会議）
- 2012年03月15日, 日本植物病理学会大会プログラム・講演要旨予稿集, 2012, 79, 日本語いもち病菌非病原力遺伝子のMultiple translocationはなぜ起こったか?欠失/再獲得仮説
- GENETICS SOC JAPAN, 2011年12月, GENES & GENETIC SYSTEMS, 86 (6), 385 - 385, 英語The DNA repair protein MoRad51 plays a role in de novo DNA methylaton of the LTR-retrotransposon MAGGY in the fungus Magnaporthe oryzae
研究発表ペーパー・要旨（国際会議）
In mythology, the Trickster is an archetype who typically behaves selfishly and delights in playing tricks and breaking ordinary rules. In many myths and folktales, however, the Trickster also brings new knowledge and, ultimately, has positive effects on the community. Transposable elements (TEs) might have played such a role in the story of genome evolution. TEs can cause nonroutine genetic events like insertional mutations and ectopic recombination that provide a fundamental source of genetic variation, but they can also be a potential threat to genome integrity. Thus, the activity of TEs is usually controlled by an array of sophisticated mechanisms for genome defense. Recent findings indicate that TEs are important components of eukaryotic genomes, often to a much larger extent than ever anticipated. In this review, I focus on the contributions of TEs to various aspects of genome evolution. In addition, why TEs are specific targets for the genome defense mechanisms is discussed.
WILEY-BLACKWELL, 2011年08月, GENES TO CELLS, 16 (8), 827 - 841, 英語[査読有り][招待有り]
書評論文,書評,文献紹介等
- 2010年02月25日, 日本植物病理学会報, 76 (1), 61, 日本語いもち病菌小分生子の細胞学的特徴
- 2009年02月25日, 日本植物病理学会報, 75 (1), 64, 日本語いもち病菌集団における非病原力遺伝子AVR‐Piaの分布
- 2009年02月25日, 日本植物病理学会報, 75 (1), 65, 日本語いもち病菌サブテロメア領域における構造変異
The discovery of RNA interference (RNAi) has been the major recent breakthrough in biology. Only a few years after its discovery, RNAi has rapidly become a powerful reverse genetic tool, especially in organisms where gene targeting is inefficient and/or time-consuming. In filamentous fungi, RNAi is not currently used as widely as is gene targeting by homologous recombination that works with practical efficiencies in most model fungal species. However, to explore gene function in filamentous fungi, RNAi has the potential to offer new, efficient tools that gene disruption methods cannot provide. In this review, possible advantages and disadvantages of RNAi for fungal biology in the postgenomics era will be discussed. In addition, we will briefly review recent discoveries on RNAi-related biological phenomena (RNA silencing) in fungi.
CURRENT BIOLOGY LTD, 2008年12月, CURRENT OPINION IN MICROBIOLOGY, 11 (6), 494 - 502, 英語[査読有り][招待有り]
書評論文,書評,文献紹介等
- 日本植物病理学会, 2008年08月25日, 日本植物病理學會報, 74 (3), 207 - 207, 日本語(153) ナシ黒斑病菌における活性酸素種(reactive oxygen species;ROS)生成遺伝子noxAおよびnoxB変異株の細胞学・病理学的比較調査(平成20年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2008年08月25日, 日本植物病理學會報, 74 (3), 205 - 205, 日本語(145) RNAサイレンシング法によるイネ科植物いもち病菌のGATA,bHLH転写因子ファミリーの機能解析(平成20年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2008年08月25日, 日本植物病理學會報, 74 (3), 171 - 171, 日本語(16) イネ科植物いもち病菌の転移因子MAGGYのコサプレッションはsiRNA依存と非依存経路によるものがある(平成20年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2008年08月25日, 日本植物病理學會報, 74 (3), 261 - 261, 日本語(354) Pseudomonas cichoriiのナスへの病原性に関するaldehyde dehydrogenase遺伝子の機能的および系統進化学的解析(平成20年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2008年08月25日, 日本植物病理学会報, 74 (3), 192 - 193, 日本語非病原力遺伝子としての機能からみたいもち病菌AVR‐Pitaの起源と進化
- 日本植物病理学会, 2008年02月20日, 日本植物病理學會報, 74 (1), 77 - 77, 日本語(80)兵庫県下で3種の宿主植物から分離されたPseudomonas fluorescens菌の系統解析(関西部会講演要旨,平成19年度地域部会講演要旨)
- 日本植物病理学会, 2008年02月20日, 日本植物病理學會報, 74 (1), 61 - 61, 日本語(20)いもち病菌のシトシンDNAメチル化は同菌の生活環において必須ではない(関西部会講演要旨,平成19年度地域部会講演要旨)
- 日本植物病理学会, 2008年02月20日, 日本植物病理學會報, 74 (1), 59 - 59, 日本語(13)ナシ黒斑病菌における活性酸素種(ROS)生成遺伝子NoxBの機能解析(関西部会講演要旨,平成19年度地域部会講演要旨)
- 日本植物病理学会, 2008年02月20日, 日本植物病理學會報, 74 (1), 61 - 61, 英語(21)Phenotypic Analyses of the Knock-down Mutants of 37 Calcium Signaling Proteins in Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)
- 日本植物病理学会, 2008年02月20日, 日本植物病理學會報, 74 (1), 61 - 61, 日本語(19)いもち病菌における非病原力遺伝子PWL1周辺構造の多型解析(関西部会講演要旨,平成19年度地域部会講演要旨)
- 日本植物病理学会, 2008年02月20日, 日本植物病理學會報, 74 (1), 62 - 62, 日本語(22)蛋白質機能改変を可能とするResurrection法の糸状菌における応用(関西部会講演要旨,平成19年度地域部会講演要旨)
- 日本植物病理学会, 2007年08月25日, 日本植物病理學會報, 73 (3), 275 - 275, 日本語(375) Pseudomonas cichorii SPC9018株のhrpクラスターと新たな病原性遺伝子(平成19年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2007年08月25日, 日本植物病理學會報, 73 (3), 211 - 211, 日本語(138) ナシ黒斑病菌の貫穿菌糸における活性酸素種(ROS)生成遺伝子NoxAの機能解析(平成19年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2007年08月25日, 日本植物病理學會報, 73 (3), 202 - 202, 日本語(103) シコクビエいもち病菌における非病原力遺伝子PWL1周辺構造の比較解析(平成19年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2007年08月25日, 日本植物病理學會報, 73 (3), 203 - 203, 英語(108) Comprehensive Analyses of Calcium Signaling Proteins in the Rice Blast Fungus, Magnaporthe oryze, by an RNA Silencing Approach(Abstracts of the Papers Presented at the Annual Meeting of the Society)
- 日本植物病理学会, 2007年08月25日, 日本植物病理学会報, 73 (3), 202 - 202, 日本語いもち病菌非病原力遺伝子AVR‐Pitaホモログのタイピング,マッピングおよび機能の比較解析
- 日本植物病理学会, 2007年08月25日, 日本植物病理学会報, 73 (3), 213 - 213, 日本語いもち病菌小型分生子の病理学的役割を解明するための基礎調査
- 日本植物病理学会, 2007年02月25日, 日本植物病理學會報, 73 (1), 57 - 57, 日本語オオムギのアワいもち病菌に対する抵抗性遺伝子の検出(関西部会講演要旨,平成18年度地域部会講演要旨)
- 日本植物病理学会, 2007年02月25日, 日本植物病理學會報, 73 (1), 57 - 57, 日本語イネいもち病菌染色体における非病原力遺伝子AVR-Pitaの移動と脱落(関西部会講演要旨,平成18年度地域部会講演要旨)
- 日本植物病理学会, 2007年02月25日, 日本植物病理學會報, 73 (1), 58 - 58, 日本語RNAサイレンシング機構は,いもち病菌における転移因子MAGGYの転移制御に関与している(関西部会講演要旨,平成18年度地域部会講演要旨)
- 日本植物病理学会, 2007年02月25日, 日本植物病理學會報, 73 (1), 57 - 58, 日本語いもち病菌における二つのDicer様蛋白質の機能分化には転写制御が関与している(関西部会講演要旨,平成18年度地域部会講演要旨)
- 日本植物病理学会, 2006年11月25日, 日本植物病理學會報, 72 (4), 238 - 239, 日本語(139) 冠さび病菌感染エンバク葉に誘導されるアベナスラミドはHR細胞に蓄積し,PRタンパク質はHR隣接細胞で発現する(平成18年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2006年11月25日, 日本植物病理學會報, 72 (4), 242 - 242, 日本語(152) 分子系統樹及び非病原力遺伝子PWL1の周辺領域の構造に基づいたシコクビエいもち病菌集団の進化過程の推定(平成18年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2006年11月25日, 日本植物病理學會報, 72 (4), 227 - 227, 英語(95) Specific Cleavage of Ribosomal RNA and mRNA during Victorin-induced Apoptotic Cell Death in Oats(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)
- 日本植物病理学会, 2006年11月25日, 日本植物病理學會報, 72 (4), 226 - 227, 日本語(94) 宿主特異的毒素ビクトリンにより誘導されるミトコンドリア膜電位差の低下は細胞死の実行段階で生じる(平成18年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2006年11月25日, 日本植物病理學會報, 72 (4), 228 - 229, 日本語(102) イネ科植物いもち病菌におけるRNAサイレンシングとDNAメチル化は独立した機構である(平成18年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2006年11月25日, 日本植物病理学会報, 72 (4), 242 - 242, 日本語いもち病菌非病原力遺伝子Avr‐Pitaの座乗染色体変動過程とその機構
- 日本植物病理学会, 2006年02月25日, 日本植物病理學會報, 72 (1), 68 - 68, 日本語いもち病菌の種特異的寄生性に関与するPWT3に対応する抵抗性遺伝子の検出とgene-for-gene関係の証明(関西部会講演要旨,平成17年度地域部会講演要旨)
- 日本植物病理学会, 2006年02月25日, 日本植物病理學會報, 72 (1), 67 - 68, 日本語比較マッピンゲによるいもち病菌の染色体構造解析(関西部会講演要旨,平成17年度地域部会講演要旨)
- 日本植物病理学会, 2006年02月25日, 日本植物病理學會報, 72 (1), 68 - 68, 日本語アワいもち病菌のイチゴツナギ亜科植物に対する非病原性の比較解析(関西部会講演要旨,平成17年度地域部会講演要旨)
- 日本植物病理学会, 2006年02月25日, 日本植物病理學會報, 72 (1), 68 - 68, 日本語イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定(関西部会講演要旨,平成17年度地域部会講演要旨)
Two RNA silencing-related phenomena, quelling and meiotic silencing by impaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are used in the quelling and MSUD pathways. Orthologs of the RNA silencing components are found in most, but not all, fungal genomes currently available in the public databases, indicating that the majority of fungi possess the silencing machinery. Advantage and disadvantage of RNA silencing as a tool to explore gene function in fungi are discussed. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2005年10月, FEBS LETTERS, 579 (26), 5950 - 5957, 英語書評論文,書評,文献紹介等
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 198 - 198, 日本語(48) Pyricularia oryzae近縁種の種分化過程のGCPSRによる解析(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 199 - 199, 日本語(51) 植物病原糸状菌Magnaporthe oryzaeにおけるRNAサイレンシング経路には二つのDicer様タンパク質のうち一つが主に関与する(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 198 - 198, 日本語(49) いもち病菌集団における非病原力遺伝子Avr-Pita座の変動機構(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 198 - 199, 日本語(50) シコクビエいもち病菌集団における非病原力遺伝子PWL1の解析(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 199 - 199, 英語(52) コムギのエンバクいもち病菌に対する抵抗性遺伝子Rwt4の野外における役割(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 300 - 300, 日本語(428) トマト転写因子Pti4, 5, 6によるPti5プロモーターの複合的な制御(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年08月25日, 日本植物病理學會報, 71 (3), 299 - 300, 日本語(427) トマト転写因子Pti5遺伝子のプロモーター配列解析(平成17年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2005年02月25日, 日本植物病理學會報, 71 (1), 54 - 54, 英語(9) Degradation of Ribosomal RNA Associated with Apoptotic Response of Oat Cells Triggered by the Host-Selective Toxin, Victorin(Abstracts Presented at the Meeting of the Kansai Division, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)
- 日本植物病理学会, 2005年02月25日, 日本植物病理學會報, 71 (1), 65 - 65, 日本語(51) コムギ品種とエンバクいもち病菌間における遺伝子対遺伝子関係の証明(関西部会講演要旨, 平成16年度地域部会講演要旨)
- 日本植物病理学会, 2005年02月25日, 日本植物病理學會報, 71 (1), 66 - 66, 英語(52) A Convergent Opposing Promoters System to Explore Genome-wide Gene Function in the Rice Blast Fungus by an RNA Silencing Approach(Abstracts Presented at the Meeting of the Kansai Division, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)
- 日本植物病理学会, 2005年02月25日, 日本植物病理學會報, 71 (1), 62 - 63, 日本語(40) シコクビエいもち病菌(Magnaporthe oryzae)の集団構造の解析(関西部会講演要旨, 平成16年度地域部会講演要旨)
- 2005年02月01日, Journal of general plant pathology : JGPP, 71 (1), 33 - 38, 英語Nitric oxide-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol of tomato bacterial wilt
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 242 - 242, 日本語(192)Arabidopsisにおける植物成長促進根圏微生物(PGPR)の遺伝子変動のマイクロアレイ解析(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 191 - 191, 日本語(4)様々な植物から分離したPyricularia属菌におけるGCPSRに基づいた種の識別(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 190 - 191, 日本語(3)いもち病菌Magnaporthe oryzaeにおけるRNAサイレンシングベクターの作製(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 194 - 194, 日本語(17)センチピードグラスに発生したいもち病菌のrDNA-ITS2領域とbeta-tubulin遺伝子の解析(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 191 - 191, 日本語(5)いもち病菌集団における染色体構造の多様性と非病原性遺伝子座の変動(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 202 - 203, 日本語(47)エンバクの抵抗性誘導におけるアポトーシス機構の解析13) : 宿主特異的毒素ビクトリンは感受性品種Iowa X469において細胞表面で認識される(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年08月25日, 日本植物病理學會報, 70 (3), 203 - 203, 日本語(48)エンバクの抵抗性誘導におけるアポトーシス機構の解析14) : エンバクにおける宿主特異的毒素ビクトリンへの結合因子のビアコアによる探索(平成16年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2004年02月25日, 日本植物病理學會報, 70 (1), 69 - 69, 英語(48)Modification of Pseudomonas fluorescens by Disrupting the Nitric Oxide Reductase (NOR) Gene for the Biological Control of Ralstonia solanacearum.(Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)
- 日本植物病理学会, 2004年02月25日, 日本植物病理學會報, 70 (1), 63 - 64, 日本語(28)GCPSRに基づいたMagnaporthe/Pyricularia属菌集団における種の識別(関西部会講演要旨)(平成15年度地域部会講演要旨)
- 日本植物病理学会, 2004年02月25日, 日本植物病理學會報, 70 (1), 64 - 64, 日本語(29)いもち病菌集団における染色体構造の多様性(関西部会講演要旨)(平成15年度地域部会講演要旨)
- 2004年, 植物病の探求． 高松進ら編 「植物病の探求」, 48-53, 日本語エンバクにおける細胞死機構研究のこれまで
その他
- 日本植物病理学会, 2003年08月25日, 日本植物病理學會報, 69 (3), 269 - 269, 日本語(135)いもち病菌の種特異的寄生性機構に関する研究(13)いもち病菌雑種後代で認められるadult resistanceに対する病原性の低下について(平成15年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2003年08月25日, 日本植物病理學會報, 69 (3), 269 - 270, 日本語(137)ペレニアルライグラスいもち病菌の地理的起源の解明とコムギに対する発病ポテンシャルの評価(平成15年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2003年08月25日, 日本植物病理學會報, 69 (3), 237 - 237, 日本語(15)いもち病菌におけるsupernumeracy chromosomeの遺伝様式と構造変異機構(平成15年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2003年08月25日, 日本植物病理學會報, 69 (3), 233 - 233, 日本語(1)いもち病菌における病原性発現物質の解析(7)エンバクいもち病菌の生産する新規毒素の白化,活性酸素生成,細胞死の誘導(平成15年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2003年08月25日, 日本植物病理學會報, 69 (3), 237 - 237, 日本語(17)いもち病菌Magnaporthe griseaにおけるsmall interfering RNAs (siRNAs)の検出(平成15年度日本植物病理学会大会講演要旨)
- (1)いもち病菌Magnaporthe griseaにおけるRNAサイレンシング
RNAサイレンシングは動物,植物,および糸状菌などの幅広い真核生物が持つ外来核酸に対する防御機構と考えられている.我々はコムギから分離されたいもち病菌Br48にGFP遺伝子を導入し,強いGFP緑色蛍光を示す株を得た.この形質転換体にGFP RNAをセンス,アンチセンス,ヘアピンなどの様々な形で転写するコンストラクトをそれぞれ導入し,GFP蛍光の増減を調査した結果,ヘアピン構造をとるGFP RNAを転写するコンストラクトが高頻度でGFP蛍光の抑制を誘導することが明らかとなった.これらGFPの発現が抑制された株ではGFP mRNAの蓄積が顕著に低下していることがノーザン解析により示された.また,GFPのサイレンシングが観察された株ではGFPヘアピンRNAに相当する3.2kbのバンドが常に観察され,両者の間に相関が認められた.以上の結果は,いもち病菌にRNAサイレンシング機構が存在することを示し,ゲノム解析が進展している本菌の逆遺伝学的な遺伝子解析に有用な手法となることが期待される.
日本植物病理学会, 2003年02月25日, 日本植物病理學會報, 69 (1), 51 - 51, 日本語 - (15)エンバクの抵抗性誘導におけるアポトーシス機構の解析 : 13)Ca^<2+>に制御されるROS生成および細胞死の細胞学的研究
植物の抵抗性発現およびアポトーシスの誘導にCa^<2+>およびROSは重要な役割を担っている.シロイヌナズナの細胞膜在性NADPHオキシダーゼAtrbohはCa^<2+>結合部位を有することが報告され,Ca^<2+>とROS生成には深い相関性があることが明らかとなっている.しかし現在までにCa^<2+>に制御されるROS生成の詳細な細胞学的解析は行われていない.そこで本研究ではカルシウムイオノフォアA23187がエンバク葉肉細胞に誘導する細胞死およびROS生成についての解析を行った.A23187処理したエンバクでは,明瞭なDNAラダー,グロマチンの凝集が認められ,EM-TUNELポジティブであり,アポトーシス様の細胞死が起こっていることが示唆された.塩化セリウム法により,細胞膜およびミトコンドリア,葉緑体,パーオキシゾームなどの細胞内小器官で強いH_2O_2の蓄積が認められた.NADPHオキシダーゼ阻害剤であるDPIを供処理すると,細胞膜でのH_2O_2蓄積および細胞死が抑制されたが,これに伴い細胞内小器官でのH_2O_2蓄積も抑制された.このことより,細胞膜でのO_2-生成が細胞内小器官での二次的なROS生成を誘導することが示唆された.
日本植物病理学会, 2003年02月25日, 日本植物病理學會報, 69 (1), 55 - 55, 日本語 - 神戸大学, 2003年, 神戸大学農学部学術報告, 27, 50 - 53, 日本語イネ科植物いもち病菌におけるRNAサイレンシング
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 176 - 177, 日本語(77)いもち病菌から単離されたgag遺伝子内に新規ドメインを持つTy3/gypsy型レトロトランスポゾンPyretの解析(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 171 - 171, 日本語(57)いもち病菌の種特異的寄生性機構に関する研究(12)エンバク・コムギいもち病菌の種レベル宿主特異性と遺伝子対遺伝子説(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 177 - 177, 日本語(78)RFLPマップによるいもち病菌染色体構造の比較解析(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 163 - 163, 日本語(27)エンバクの抵抗性誘導におけるアポトーシス機構の解析 11)非親和性冠さび病菌の被侵入細胞における過敏感死はアポトーシス様である(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 163 - 164, 日本語(28)エンバクの抵抗性誘導におけるアポトーシス機構の解析 12)ビクトリン処理により誘導されるヌクレアーゼの精製と諸性質について(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 164 - 164, 日本語(29)いもち病菌における病原性発現物質の解析(6)エンバクいもち病菌の生産する白化誘導毒素の単離・精製(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年08月25日, 日本植物病理學會報, 68 (2), 243 - 243, 日本語(324)レタス腐敗病の発病過程におけるアポトーシス様細胞死の関与(平成14年度 日本植物病理学会大会講演要旨)
- 日本植物病理学会, 2002年04月25日, 日本植物病理學會報, 68 (1), 90 - 90, 日本語いもち病菌における転移因子クラスターの構造解析(関西部会講演要旨)
- 日本植物病理学会, 2002年04月25日, 日本植物病理學會報, 68 (1), 90 - 90, 日本語エンバク/コムギいもち病菌の温度依存的種特異的寄生性とそれに関与する遺伝子の連鎖マーカーの探索(関西部会講演要旨)
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 181 - 181, 日本語(236)Pyricularia属菌集団における非病原性遺伝子AVR1-CO39の分布と構造
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 181 - 181, 日本語(235)いもち病菌の種特異的寄生性機構に関する研究(11)アワ菌×コムギ菌の雑種後代におけるアワに対する病原性の遺伝解析
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 122 - 122, 日本語(12)エンバク初生葉の老化過程において認められる病害抵抗性の増強
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 122 - 122, 日本語(11)エンバクの抵抗性誘導におけるアポトーシス機構の解析(10)宿主特異的毒素ビクトリンの毒性発現機構における抵抗性反応の介在
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 130 - 130, 日本語(42)交配時の反復遺伝子不活性化機構RIPのいもち病菌における存在
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 121 - 121, 日本語(9)エンバクのファイトアレキシンの生合成酵素遺伝子のクローニング
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 121 - 122, 日本語(10)エンバクの抵抗性誘導におけるアポトーシス機構の解析(9)ミトコンドリアの役割について
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 129 - 130, 日本語(41)MAGGYのいもち病菌における転移の抑制はメチル化が主要因ではない
- 日本植物病理学会, 2001年08月25日, 日本植物病理學會報, 67 (2), 136 - 137, 日本語(67)エンバクレトロトランスポゾンOARE-1のムギ類における分布と病原菌感染時における発現挙動
- Involvement of gacA Gene in the Suppression of Tomato Bacterial Wilt by Pseudomonas fluorescens FPT9601 :
Pseudomonas fluorescens FPT9601, a plant growth-promoting rhizobacterium (PGPR) isolated from tomato rhizosphere, can protect tomato (Lycopersicon esculentum Mill) from bacterial wilt disease caused by Ralstonia solanacearum. This strain produces antibiotics 2,4-diacetylphloroglucinol (2,4-DAPG) and hydrogen cyanide (HCN). It also produces proteases and uncharacterized siderophores (Sid). A mutant strain SM2214, obtained by Tn5 insertion, did not produce 2,4-DAPG, HCN or proteases, but overproduced Sid. Marker-exchange mutagenesis confirmed that a single transposon insertion caused the multiple phenotypic changes of this mutant. Complementation of the mutant with a 1.3-kb DNA fragment that was amplified from genomic DNA of the wild-type P. fluorescens strain by PCR could restore the lost functions of the mutant strain. Nucleotide sequencing revealed that the fragment contained a 642-bp open reading frame (ORF) highly homologous to the regulator responser gene gacA. The in vitro anti-bacterium test and plant protection experiment under greenhouse conditions indicated that the gacA gene played an important role in the suppression of tomato bacterial wilt disease. (Received November 20, 2000 ; Accepted January 19, 2001)
Phytopathological Society of Japan, 2001年, Journal of general plant pathology : JGPP, 67 (2), 134 - 143, 英語 - 日本植物病理学会, 2000年12月25日, 日本植物病理學會報, 66 (3), 288 - 289, 日本語エンバクの抵抗性誘導におけるアポトーシス機構の解析 : 8)EM-TUNEL法によるアポトーシス細胞の確認(関西部会講演要旨)
- 日本植物病理学会, 2000年12月25日, 日本植物病理學會報, 66 (3), 281 - 281, 日本語いもち病菌の種特異的寄生性機構に関する研究(9) : イネ菌×コムギ菌の雑種後代におけるコムギに対する病原性の遺伝解析(関西部会講演要旨)
- 日本植物病理学会, 2000年12月25日, 日本植物病理學會報, 66 (3), 281 - 282, 日本語いもち病菌の種特異的寄生性機構に関する研究(10) : Brachiaria菌×コムギ菌のF_1における病原性の遺伝解析(関西部会講演要旨)
- 日本植物病理学会, 2000年12月25日, 日本植物病理學會報, 66 (3), 291 - 291, 日本語エンバクレトロトランスポゾンのクローニングとその転写活性(関西部会講演要旨)
- 日本植物病理学会, 2000年12月25日, 日本植物病理學會報, 66 (3), 281 - 281, 日本語いもち病菌における病原性発現物質の解析 : (5)コムギおよびエンバクいもち病菌の生産する病原性因子(関西部会講演要旨)
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 120 - 120, 日本語いもち病菌の種特異的寄生性機構に関する研究 : (8) エンバク菌を用いた解析系の確立(平成12年度 日本植物病理学会大会 講演要旨)
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 120 - 120, 日本語いもち病菌における病原性発現物質の解析(4)ピリカラシンH産性能のDigitaria属分離菌における普遍性について(平成12年度 日本植物病理学会大会 講演要旨)
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 120 - 120, 日本語キビいもち病菌に見られる転移因子のRIP様機構による退化(平成12年度 日本植物病理学会大会 講演要旨)
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 137 - 137, 英語Cloning of GacA Gene from Pseudomonas fluorescens FPT9601
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 113 - 113, 日本語エンバクの抵抗性誘導におけるアポトーシス機構の解析 : 7) : ウイルス, 細菌, 糸状菌の感染によるアポトーシス誘導(平成12年度 日本植物病理学会大会 講演要旨)
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 119 - 120, 日本語いもち病菌の種特異的寄生性機構に関する研究(7) : イネ科植物異族間で保存されたいもち病抵抗性システムの存在について(平成12年度 日本植物病理学会大会 講演要旨)
- 日本植物病理学会, 2000年08月25日, 日本植物病理學會報, 66 (2), 113 - 113, 日本語エンバクの抵抗性誘導におけるアポトーシス機構の解析 : 6)DNAラダー化とクロマチン凝集に介在する細胞内シグナルについて(平成12年度 日本植物病理学会大会 講演要旨)
- Signal Mediators for Phytoalexin Production in Defense Response of Oats Elicited by Victorin as a Specific Elicitor :
Signals mediating phytoalexin(PA)production were analyzed in primary leaves of oats cv.Iowa X469 treated with an elicitor victorin. Production of the PA avenanthramide A was inhibited by DPI, an inhibitor of NADPH oxidase/nitric oxide synthase(NOS)and the NOS inhibitors L-NMMA and 1, 3-PBIT. However, catalase and superoxide dismutase (SOD)hardly suppressed it. From the data, NO functions as a major reactive oxygen species in signal transduction leading to PA production in the defense response of oats. EGTA, verapamil and ruthenium red inhibited PA production, suggesting that Ca^<2+> influx into the cytoplasm and intracellular Ca^<2+> movement are involved in the defense response. Trifluoperazine, a calmodulin function inhibitor, and K-252a, a serine/threonine kinase inhibitor, also suppressed the accumulation, whereas okadaic acid, a serine/threonine phosphatase inhibitor, did not suppress it, suggesting the involvement of calmodulin and protein kinase, but not of phosphatase in PA production.
Phytopathological Society of Japan, 2000年, Journal of general plant pathology : JGPP, 66 (3), 185 - 190, 英語 - Pathogenicity, Mating Ability and DNA Restriction Fragment Length Polymorphisms of Pyricularia Populations Isolated from Gramineae, Bambusideae and Zingiberaceae Plants :
Eighty-five Pyricularia isolates were collected from 29 host species of Gramineae, Bambusideae and Zingiberaceae plants sampled in Brazil, Uganda, Ivory Coast, India, Nepal, China, Indonesia and Japan.These isolates were compared on the basis of pathogenicity, mating ability and restriction fragment length polymorphisms with single-copy DNA probes.Based on the pathogenicity to eight differential gramineous plants, these isolates were classified into seven pathotypes:finger millet type, foxtail millet type, common millet type, rice type, crabgrass type, Italian ryegrass / weeping lovegrass type, and non-cereal / grass type.Genetic variation among these isolates was assessed by RFLP analysis with two restriction enzymes and nine single-copy DNA probes isolated from a finger millet strain.An UPGMA dendrogram based on the RFLPs revealed that the 85 isolates could be classified into seven major groups.Isolates from cereal crops(finger millet, foxtail millet, common millet, wheat and rice)and a grass, Brachiaria plantaginea, were clustered into a single group.They were further divided into six subgroups corresponding to the pathotypes.Among cereal crop isolates only an isolate from pearl millet was located into a different group.The remaining isolates were clustered into five groups designated as the crabgrass group, the buffelgrass and jungle rice group, the rice cutgrass, knotroot bristlegrass and Setaria tomentosa group, the bamboo and bamboo grass group and the Zingiber mioga group.The isolates from cereal crops were generally capable of mating with finger millet strains and constituted a closed mating compatibility group.These results suggested that the isolates from cereal crops from a single group with a common ancestor although they are pathogenic to taxonomically diverse plants.A combined analysis of the pathogenicity and genetic similarity suggested that the transmission of M.grisea isolates occurs in natural agroecosystems between finger millet and Eleusine africana, goosegrass or Bambusa arundinacea, between foxtail millet and green bristlegrass, and between rice and tail fescue, Italian ryegrass, sweet vernalgrass, reed canarygrass or Oryza longistaminata.
Phytopathological Society of Japan, 2000年, Journal of general plant pathology : JGPP, 66 (1), 30 - 47, 英語 - 日本植物病理学会, 1999年12月25日, 日本植物病理學會報, 65 (6), 673 - 673, 日本語(10) ゴルフ場のペレニアルライグラスに発生したいもち病菌の由来 (2) : DNAフィンガープリントおよびrDNAの解析 (関西部会講演要旨)
- 日本植物病理学会, 1999年12月25日, 日本植物病理學會報, 65 (6), 691 - 691, 日本語(83) エンバクの抵抗性誘導におけるアポトーシス機構の解析 5) : PA蓄積およびDNAラダー化に対するH_2O_2およびNOの相反する影響 (関西部会講演要旨)
- 日本植物病理学会, 1999年12月25日, 日本植物病理學會報, 65 (6), 690 - 690, 日本語(79) いもち病菌の種特異的寄生性機構に関する研究 (6) : アワ菌×コムギ菌の雑種後代のコムギに対する病原性に関与する遺伝子の同定とその宿主細胞反応における機能の解析 (関西部会講演要旨)
- 日本植物病理学会, 1999年12月25日, 日本植物病理學會報, 65 (6), 691 - 691, 日本語(82) ポリアミンによるエンバクのファイトアレキシン蓄積阻害とNO生成代謝系との関係について (関西部会講演要旨)
- 核リボソームDNA多型とレトロトランスポゾンMAGGYの分布からみた見たいもち病菌集団の遺伝的多様性
種々のイネ科植物から分離したいもち病菌菌株間の遺伝的相互関係を明らかにするため,核リボソームDNA(rDNA)の解析を行った。各菌株の全ゲノムDNAを2種類の4塩基認識制限酵素で切断して多型解析を行った結果,供試71菌株が13種類のrDNAタイプに類別された。このRFLPデータならびにITS2領域の塩基配列に基づいてデンドログラムを作成したところ,主要なイネ科栽培植物(イネ,アワ,キビ,シコクビエ,コムギ,エンバク)とその近縁種の病原菌は単一クラスターを形成した。一方,メヒシバ菌,ブッフェルグラス菌等はデンドログラム上において上記栽培植物寄生菌群の外側に位置し,日本産タケ・ササ菌はさらに遠縁であることが明らかとなった。つぎに,これらの菌株にかけるレトロトランスポゾンMGGYの分布を調べたところ,MAGGYを多コピー保有するイネ菌・アワ菌等の菌株は,ほぼ栽培植物寄生菌群クラスターの中の単一サブクラスターに属することが明らかになった。一方,このサブクラスターから遠縁のブッフェルグラス菌にも,MAGGYが多コピー見出された。このことから,過去にMAGGYの水平移行が起こったことが示唆された。
日本植物病理学会, 1999年12月25日, 日本植物病理學會報, 65 (6), 588 - 596, 英語 - ベトナム産イネいもち病菌の個体群構造
ベトナムの主要な2つの稲作地帯, Red River Delta(北部)およびMekong River Delta(南部)から採集したイネいもち病菌計78菌糸を用いて, 両地域における本病菌の個体群構造を比較検討した。レトロトランスポゾンMAGGYをプローブとしたDNAフィンガープリンティングの結果, 北部の菌株は4つのリネージ(VL1-VL4)に分かれたが, 南部の菌株はこれらとは異なる単一リネージ(VL5)に分類された。このことから, 北部の個体群の方が遺伝的多様性に富むことが示唆された。さらに, 判別品種を用いた病原性検定の結果, 両集団におけるレース構成は大きく異なり, 共通のレースは見いだされなかった。以上のことから, 遺伝的にも病原性においても両個体群の構造は大きく異なることが示唆された。
日本植物病理学会, 1999年08月25日, 日本植物病理學會報, 65 (4), 475 - 479, 英語 - ブラジルにおけるコムギいもち病菌個体群のレトロトランスポゾンMGR583ホモログによる分子解析
コムギいもち病菌個体群の分子解析のためのプローブとして, シコクビエいもち病菌のゲノムから散在反復配列のファミリーをクローニングした。この因子はコムギいもち病菌にゲノムあたり30から40コピー存在した。シークエンスの結果, 本因子はポリAタイプレトロトランスポゾンMGR583の逆転写酵素ドメインと高い相同性を有することが判明した。本因子をプローブとしてコムギいもち病菌個体群のフィンガープリント解析を行ったところ, 本菌群はそれ自身で独立かつ単一のリネージを形成していることが明らかとなり, 単一起源であることが示唆された。
日本植物病理学会, 1999年08月25日, 日本植物病理學會報, 65 (4), 429 - 436, 英語 - 日本植物病理学会, 1999年06月25日, 日本植物病理學會報, 65 (3), 342 - 342, 日本語(85) ゴルフ場のペレニアルライグラスに発生したいもち病菌の由来 : 宿主範囲, 交配能, DNAフィンガープリント型からの一考察 (平成11年度 日本植物病理学会大会)
- 日本植物病理学会, 1999年06月25日, 日本植物病理學會報, 65 (3), 341 - 342, 日本語(82) イネいもち病菌に存在するMAGGYの転移活性化機構 : β-glucuronidase (GUS)遺伝子を用いたプロモーター活性の解析 (平成11年度 日本植物病理学会大会)
- 日本植物病理学会, 1999年06月25日, 日本植物病理學會報, 65 (3), 328 - 329, 日本語(32) いもち病菌における病原性発現物質の解析 : (3)メヒシバいもち病菌の産生するピリカラシンHの感染葉における検出と抵抗性抑制 (平成11年度 日本植物病理学会大会)
- 日本植物病理学会, 1999年06月25日, 日本植物病理學會報, 65 (3), 321 - 321, 日本語(1) エンバクの抵抗性誘導におけるアポトーシス機構の解析 : (3)ビクトリンにより活性化されるヌクレアーゼ (平成11年度 日本植物病理学会大会)
- 日本植物病理学会, 1999年06月25日, 日本植物病理學會報, 65 (3), 321 - 321, 日本語(2) エンバクの抵抗性誘導におけるアポトーシス機構の解析 : (4)核の電子顕微鏡観察 (平成11年度 日本植物病理学会大会)
- 日本におけるイネいもち病菌個体群構造のDNAフィンガープリント解析
日本におけるイネいもち病菌の個体群構造を明らかにするため, MAGGYおよびMGR586を用いてDNAフィンガープリント解析を行った。1993〜1997年に採集・単胞子分離した278菌株(圃場菌株)および1976年に採集された22菌株(実験室保存菌株)を解析したところ, それぞれの菌株集団が70%レベルの相同性を有する2つのリネージ(Lineage)に分けられた。さらに圃場菌株における2つのリネージは実験室保存菌株における2つのリネージと1対1に対応した。以上の結果から, 現在の日本におけるイネいもち病菌集団には2つのリネージ(JL1,JL2と仮称)が存在することが明らかとなった。JL1は圃場菌株および実験室保存菌株のそれぞれ97%および77%を占める主要なリネージで, 日本全国に分布していた。一方, 1960年以前に採集された集団は少なくとも5つのリネージよりなるとされているが, JLl, JL2はそのうちの2つに相当した。このことから, 日本のイネいもち病菌集団の構造は1960年から1976年の間に大きく変化したことが示唆された。各リネージに属する菌系のイネ判別品種に対する病原性を調べたところ, リネージと病原性の間に関連は認められなかった。両プローブによるリネージ分類はほぼ同じであったことから, MAGGYはイネいもち病菌の個体群動態を解析するためのプローブとして有用と考えられる。
日本植物病理学会, 1999年02月25日, 日本植物病理學會報, 65 (1), 15 - 24, 英語 - 日本植物病理学会, 1998年12月25日, 日本植物病理學會報, 64 (6), 616 - 616, 日本語(5) いもち病菌の種特異的寄生性機構に関する研究(5)アワ菌とコムギ菌の雑種後代におけるMAGGYの転移の確認並びに各種散在反復配列の転移活性 (平成10年度関西部会)
- 日本植物病理学会, 1998年12月25日, 日本植物病理學會報, 64 (6), 615 - 616, 英語(4) イネいもち病菌の系譜のDNAフィンガープリント解析(3)ベトナムにおける圃場分離株の個体群構造 (平成10年度関西部会)
- 日本植物病理学会, 1998年12月25日, 日本植物病理學會報, 64 (6), 624 - 625, 日本語(39) 形質転換により導入したレトロトランスポゾンMAGGYの新宿主細胞における増幅の動向について (平成10年度関西部会)
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 350 - 351, 日本語(95) いもち病菌における病原性発現物質の解析(2)コムギいもち病菌の生産する植物毒素
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 353 - 353, 日本語(104) エンバクの抵抗性誘導におけるアポトーシス機構の解析(1)感染およびエリシターによる誘導
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 351 - 351, 日本語(96) キビいもち病菌に存在するMAGGY様配列のメチル化による転移制御の可能性
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 351 - 351, 日本語(97) イネいもち病菌の系譜のDNAフィンガープリント解析(2)日本における圃場分離株の個体群構造
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 353 - 353, 日本語(106) エンバクのPR-タンパク質であるキチナーゼとそれにより冠さび病菌感染菌糸から遊離するキチンオリゴ糖のエリシター活性について
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 353 - 354, 日本語(107) エンバク冠さび病における抵抗性発現時に活性化する遺伝子群の解析
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 351 - 352, 日本語(99) Pyricularia属菌における各種転移因子および2本鎖RNAの分布
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 352 - 352, 日本語(100) いもち病菌の種特異的寄生性機構に関する研究(3)アワ菌×コムギ菌の雑種後代におけるコムギに対する病原性の遺伝解析
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 352 - 352, 日本語(101) いもち病菌の種特異的寄生性機構に関する研究(4)アワ菌とコムギ菌の雑種後代を用いたRFLP地図の作成
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 350 - 350, 日本語(94) いもち病菌における病原性発現物質の解析(1)いもち病菌のピリカラシンH生産とメヒシバに対する病原性
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 353 - 353, 日本語(105) エンバクの抵抗性誘導におけるアポトーシス機構の解析(2) Ca^<2+>, 活性酸素, プロテアーゼおよびエンドヌクレアーゼの介在
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 351 - 351, 日本語(98) コムギいもち病菌の個体群構造ならびに起源の分子マーカーによる解析
- 日本植物病理学会, 1998年08月25日, 日本植物病理學會報, 64 (4), 354 - 354, 日本語(108) RT-PCRによるエンバクのレトロトランスポゾンのクローニング
- DNAフィンガープリンティング分析により示唆されたイネいもち病菌の野生イネ科植物への自然感染
MAGGYをブローブとしたDNAフィンガープリント分析により, 世界各地から採集したイネいもち病菌と日本産野生イネ科植物いもち病菌との遺伝的類縁関係を調査した。供試菌から得られたDNAフィンガープリントに基づきクラスター分析を行ったところ, 日本産と外国産イネ菌の類縁性は, 27%以下であった。一方, 日本産ネズミムギ, ハルガヤ, クサヨシ, オニノウシノケグサ各菌は日本産イネ菌に対して80%以上の高い類縁性を示した。以上の結果から, これら日本産の野生イネ科植物の菌はイネ菌がこれら植物に感染したものであると推定される。
日本植物病理学会, 1998年04月25日, 日本植物病理學會報, 64 (2), 125 - 128, 英語 - 日本植物病理学会, 1997年12月25日, 日本植物病理學會報, 63 (6), 507 - 508, 日本語(4) 形質転換により導入したレトロトランスポゾンMAGGYの各種いもち病菌における転移活性 (関西部会)
- 日本植物病理学会, 1997年12月25日, 日本植物病理學會報, 63 (6), 508 - 508, 日本語(5) MAGGYをプローブとしたDNAフィンガープリンティングによるイネいもち病菌の野生イネ科植物への移行に関する解析 (関西部会)
- 日本植物病理学会, 1997年06月25日, 日本植物病理學會報, 63 (3), 245 - 245, 日本語(216)4塩基認識制限酵素を用いたリボソームRNA遺伝子のRFLP分析によるイネ科植物いもち病菌の遺伝的類縁性の解析(平成9年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 1997年06月25日, 日本植物病理學會報, 63 (3), 245 - 245, 日本語(217)いもち病菌におけるレトロトランスポゾンMAGGYの分布 : 特にその水平移行の可能性について(平成9年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 1997年06月25日, 日本植物病理學會報, 63 (3), 246 - 246, 日本語(220)いもち病菌の種特異的寄生性機構に関する研究 : II. アワ菌×コムギ菌の雑種後代におけるMAGGY遺伝子座の再編成(平成9年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 1997年06月25日, 日本植物病理學會報, 63 (3), 245 - 246, 英語(218)イネいもち病菌の系譜のDNAフィンガープリント解析 : I. 日本7県から分離した菌系のMAGGYプローブによる解析(平成9年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 1997年06月25日, 日本植物病理學會報, 63 (3), 246 - 246, 日本語(219)いもち病菌におけるレトロトランスポゾン,MAGGYの転移の活性化と細胞内複合体の形成(平成9年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 1997年06月25日, 日本植物病理學會報, 63 (3), 230 - 230, 日本語(156)イネいもち病菌分生胞子発芽液中に存在する植物毒素(平成9年度日本植物病理学会大会講演要旨)
- 日本植物病理学会, 1996年12月25日, 日本植物病理學會報, 62 (6), 622 - 623, 日本語(24) Biolistic法によりKurthia zopfii由来のキチナーゼ遺伝子を導入したトランスジェニックイチゴにおけるイチゴ炭疽病菌に対する抵抗性の増強 (関西部会)
- 日本植物病理学会, 1996年12月25日, 日本植物病理學會報, 62 (6), 617 - 617, 日本語(1) イネ科植物いもち病菌からのリボソームRNA遺伝子の単離 (関西部会)
- 日本植物病理学会, 1996年06月25日, 日本植物病理學會報, 62 (3), 290 - 291, 日本語(123) エンバク冠さび病菌細胞壁からキチナーゼにより遊離するエリシター活性物質について (日本植物病理大会)
- 日本植物病理学会, 1996年06月25日, 日本植物病理學會報, 62 (3), 293 - 293, 日本語(133) キビいもち病菌ゲノムに存在するレトロトランスポゾン様配列の転移の活性化 (日本植物病理大会)
- 日本植物病理学会, 1996年06月25日, 日本植物病理學會報, 62 (3), 293 - 293, 日本語(132) キビいもち病菌ゲノムに存在するMAGGYと相同性を持つレトロトランスポゾン様配列 (日本植物病理大会)
- 日本植物病理学会, 1996年06月25日, 日本植物病理學會報, 62 (3), 293 - 294, 日本語(135) いもち病菌の寄生性分化に関する研究 : 10. RFLP分析による菌糸の解析 (4) (日本植物病理大会)
- 日本植物病理学会, 1996年06月25日, 日本植物病理學會報, 62 (3), 288 - 288, 日本語(114) いもち病菌の種特異的寄生性に関する研究II. : アワいもち病菌胞子発芽液中の種特異的病原性発現物質について (日本植物病理大会)
- 日本植物病理学会, 1996年06月25日, 日本植物病理學會報, 62 (3), 293 - 293, 日本語(134) いもち病菌の種特異的寄生性機構に関する研究 : 1. アワ菌・コムギ菌の雑種後代における病原性とレトロトランスポゾンの分析 (日本植物病理大会)
- Pyricularia 属菌におけるレトロトランスポゾン MAGGY の分布
さまざまな植物から分離されたいもち病菌におけるレトロトランスポゾンMAGGYの分布を調査した。本因子はイネ菌, アワ菌, エノコログサ菌には多コピー存在したが, コムギ菌, シコクビエ菌, オヒシバ菌, メヒシバ菌, Digitaria horizontalis菌, およびミョウガ菌には全く存在しなかった。以上のことから, イネ菌はアワ菌ならびにエノコログサ菌と近縁であることが示唆された。
日本植物病理学会, 1995年12月25日, 日本植物病理學會報, 61 (6), 549 - 554, 英語 - 日本植物病理学会, 1995年06月25日, 日本植物病理學會報, 61 (3), 231 - 231, 日本語(88) いもち病菌の寄生性分化に関する研究 : 9. RFLP 分析による菌系の解析 (3) (日本植物病理大会)
- 日本植物病理学会, 1995年06月25日, 日本植物病理學會報, 61 (3), 231 - 231, 日本語(89) レトロトランスポゾンMaggyの各種イネ科植物寄生性いもち病菌における分布 (日本植物病理大会)
- 日本植物病理学会, 1995年06月25日, 日本植物病理學會報, 61 (3), 234 - 234, 日本語(100) エンバク冠さび病抵抗性およびビクトリン感受性遺伝子Pc-2/Vbに連鎖するRAPDマーカーの同定について (日本植物病理大会)
- 日本植物病理学会, 1995年06月25日, 日本植物病理學會報, 61 (3), 250 - 250, 日本語(164) 各種イネ科植物いもち病菌の寄生性に関する研究 : 3. イネいもち病菌分生胞子発芽液中に存在する病原性因子 (日本植物病理大会)
- 日本植物病理学会, 1995年06月25日, 日本植物病理學會報, 61 (3), 246 - 246, 日本語(148) エンバクの細胞膜ATPase活性に対する特異的および非特異的エリシターの影響 (日本植物病理大会)
- 日本植物病理学会, 1995年06月25日, 日本植物病理學會報, 61 (3), 246 - 246, 日本語(149) エリシター処理により誘導されるエンバクのキチナーゼについて (日本植物病理大会)
- 日本植物病理学会, 1994年12月25日, 日本植物病理學會報, 60 (6), 769 - 769, 日本語(16) RAPD マーカーによるエンバク冠さび病抵抗性およびビクトリン感受性遺伝子の Pc-2/Vb タギング (関西部会)
- 日本植物病理学会, 1994年12月25日, 日本植物病理學會報, 60 (6), 769 - 770, 英語(17) Establishment of a Suspension Culture System of Oat Cells for Avenalumin Production Induced by Various Elicitors
- 日本植物病理学会, 1994年12月25日, 日本植物病理學會報, 60 (6), 769 - 769, 日本語(15) エンバク冠さび病における菌感染部周縁細胞の酵素耐性機構 : 壁結合性ジフェルラ酸形成とパーオキシダーゼの役割 (関西部会)
- 日本植物病理学会, 1994年06月25日, 日本植物病理學會報, 60 (3), 384 - 384, 日本語
- 日本植物病理学会, 1994年06月25日, 日本植物病理學會報, 60 (3), 346 - 347, 日本語(117) いもち病菌の寄生性分化に関する研究8. : RFLP分析による菌糸の解析 (2) (日本植物病理学会大会)
- カリフラワーモザイクウイルスのオープンリーディングフレームI変異体のコマツナプロトプラストにおける増殖
クローニングされたカリフラワーモザイクウイルス(CaMV)DNAを, トランスアクテイベーターとして機能することが知られているCaMVのオープンリーディングフレーム(ORF)VIの産物を発現するプラスミドとともに, ポリエチレングリコール法を用いてコマツナプロトプラストに接種することにより, 最高約50%の感染率が得られた。また, 感染プロトプラストからは, ウイルスタンパク質, および子孫ウイルスDNAも検出可能となった。この系を用い, 植物体で感染性を失った3種のCaMVのORF I挿入変異体の, コマツナプロトプラストへの感染性を調べたところ, ウイルスDNA, タンパク質ともに, 野生株と同程度に蓄積していることが確認された。この結果は, CaMVのORF I産物が, ウイルスの細胞間移行性に関与するタンパク質であり, CaMVの一細胞での複製, 増殖には関与しないということを強く支持するものである。
日本植物病理学会, 1994年02月25日, 日本植物病理學會報, 60 (1), 27 - 35, 英語 昆虫媒介性カリフラワーモザイクウイルス日本分離株CaMV-M系統をクローニングし, そのORF II領域の塩基配列を決定した。非昆虫媒介性であるCM1841系統と比較した結果, ORF II内に9個の塩基置換があり, そのうち4個はアミノ酸置換を生じていた。CaMV-M系統とCM1841系統間で, ORF II 内前半領域のか巾in vitro組換え体を作製し, その昆虫媒介性を検定したところ, 組換え体の媒介性はORF II 前半領域がどちらの系統に由来するものかで決定されていた。ORF内をCaMV-Mと組み換えることにより, CM1841系統に昆虫媒介性を付与できることから, CM1841系統が非昆虫媒介性であるのは, 発現を制御する上流配列が原因なのでほなく, ORF II 内の塩基置換が原因であることがわかった。また, CM1841系統とCM1841系統にCaMV-M系統のORF II前半領域を導入した組換え体の感染葉中のORF II の量を比較したところ, 相換え体ではCM1841系統より多量の産物が存在していることが明らかとなった。
日本植物病理学会, 1991年12月25日, 日本植物病理學會報, 57 (5), 634 - 640, 英語
書籍等出版物
- 共著, 日本植物病理学会, 2022年08月イネ科植物いもち病菌のヒストン修飾機構とゲノム進化
- 単著, 講談社, 2022年04月14日, 日本語, ISBN: 4065277663遺伝子とは何か? 現代生命科学の新たな謎 (ブルーバックス)
- 共著, 細菌とバクテリオファージの遺伝学, 化学同人, 2021年01月, 日本語, ISBN: 9784759820485エッセンシャル遺伝学・ゲノム科学
- 共著, 講談社, 2019年03月, 日本語植物たちの戦争
一般書・啓蒙書
- 単著, 講談社, 2019年03月, 日本語科学と非科学 その正体を探る
一般書・啓蒙書
- 単著, 講談社現代新書, 2016年03月, 日本語
一般書・啓蒙書
- 共著, Springer-Verlag, 2015年01月, 英語RNA silencing in filamentous fungi: from basic to applications
学術書
- 単著, 講談社現代新書, 2014年06月, 日本語生命のからくり
一般書・啓蒙書
- 共著, 秀潤社, 2004年, 日本語細胞死の新展開. 新版 分子レベルからみた植物の耐病性 島本功、渡辺雄一郎編 pp.118-125
学術書
- 共著, 日本植物病理学会, 2004年, 日本語いもち病菌におけるRNAサイレンシング機構 自他識別と応答のバイオフロンティア 植物感染生理談話論文集. 高橋英樹,羽柴輝良編 pp.53-62
学術書
- 共著, Kluwer academic pub., 2004年, 英語RNA silencing in the phytopathogenic fungus Magnaporthe grisea. In: Rice Blast: Interaction with Rice and Control(eds. S. Kawasaki) pp.15-21
学術書
講演・口頭発表等
- The 8th International Rice Blast Conference, 2019年05月, 英語, Chingdu, China, 国際会議Spatio-temporal regulation of morphological differentiation in Pyricularia oryzae.
ポスター発表
- 平成31年度日本植物病理学会大会, 2019年03月, 日本語, つくば国際会議場, 国内会議コムギいもち病菌の生産する小分子RNAの体系的な特徴付け
口頭発表（一般）
- 平成31年度日本植物病理学会大会, 2019年03月, 日本語, つくば国際会議場, 国内会議コムギいもち病菌Ourmia-like virusesの2種のサテライトRNA様因子
口頭発表（一般）
- 平成31年度日本植物病理学会大会, 2019年03月, 日本語, つくば国際会議場, 国内会議Olpidium virulentusの休眠胞子におけるミラフィオリレタスビックベインウイルスの局在
口頭発表（一般）
- 日本共生生物学会 第２回大会, 2018年11月, 日本語, 神戸大学, 国内会議ヴァイロコントロールー菌類ウイルスを利用した病害防除法ー
ポスター発表
- 日本共生生物学会 第２回大会, 2018年11月, 日本語, 神戸大学, 国内会議いもち病菌の組織特異的感染戦略
ポスター発表
- 平成30年度日本植物病理学会関西部会, 2018年09月, 日本語, 山口大学, 国内会議ヒストンメチル基転移酵素MoSET1が制御するいもち病菌リン酸化酵素の機能解析
口頭発表（一般）
- 平成30年度日本植物病理学会関西部会, 2018年09月, 日本語, 山口大学, 国内会議いもち病菌のイネ根における宿主特異的寄生性評価
口頭発表（一般）
- 平成30年度日本植物病理学会大会, 2018年03月, 日本語, 神戸国際会議場, 国内会議いもち病菌の脂質・糖代謝における付着器形成シグナル経路の関与．
口頭発表（一般）
- 平成29年度 日本植物病理学会関西部会, 2017年09月, 日本語, 大阪府立大学, 国内会議MoAgo3アルゴノートはMoAgo1とのsiRNAの競合によりRNAサイレンシングを負に制御する
口頭発表（一般）
- 平成29年度 日本植物病理学会関西部会, 2017年09月, 日本語, 大阪府立大学, 国内会議Building blocks法によるいもち病菌の脂質代謝遺伝子群の機能解析
口頭発表（一般）
- 平成２９年度日本植物病理学会大会, 2017年04月, 日本語, 盛岡, 国内会議土壌からのミラフィオリレタスビックベインウイルスのqRT-PCR法による検出
口頭発表（一般）
- 平成２９年度日本植物病理学会大会, 2017年04月, 日本語, 盛岡, 国内会議Olpidium virulentusの遊走子放出に及ぼす土壌pHの影響
口頭発表（一般）
- 7th International Rice Blast Conference, 2016年10月, 英語, The Bellevue Manila, Philippines, 国際会議Three argonaute protein and their associated sRNAs in Pyricularia oryzae
口頭発表（一般）
- 7th International Rice Blast Conference, 2016年10月, 英語, The Bellevue Manila, Philippines, 国際会議Hydrophobin in a wheat isolate of Pyricularia oryzae: Mpg1, but not Mhp1, is essential for adhesion and pathogenicity
ポスター発表
- 日本植物病理学会関西部会, 2016年09月, 日本語, 静岡県コンベンションアーツセンター グランシップ, 国内会議白紋羽病菌におけるウイルス伝搬能を獲得した変異株の細胞学的解析
口頭発表（一般）
- 日本植物病理学会関西部会, 2016年09月, 日本語, 静岡県コンベンションアーツセンター グランシップ, 国内会議コムギいもち病菌における3種の新規ourmiavirus様マイコウイルスの同定
口頭発表（一般）
- 日本植物病理学会関西部会, 2016年09月, 日本語, 静岡県コンベンションアーツセンター グランシップ, 国内会議いもち病菌におけるオートファジー誘導機構の解明（２）オートファジーと付着器形成過程におけるシグナル経路の検証
口頭発表（一般）
- 日本植物病理学会関西部会, 2016年09月, 日本語, 静岡県コンベンションアーツセンター グランシップ, 国内会議イネ科植物いもち病菌ゲノムに存在する3種のArgonauteにロードされるsRNAの比較解析
口頭発表（一般）
- 平成28年度植物感染生理談話会, 2016年08月, 日本語, シーパル須磨（神戸）, 国内会議糸状菌におけるウイルス伝搬能を獲得した変異株の特性解析
ポスター発表
- 平成28年度植物感染生理談話会, 2016年08月, 日本語, シーパル須磨（神戸）, 国内会議オートファジーと付着器形成過程におけるシグナル経路の解明
ポスター発表
- 平成28年度日本植物病理学会大会, 2016年03月, 日本語, 岡山コンベンションセンター, 国内会議いもち病菌におけるオートファジー誘導機構の解明（１）各種シグナル物質添加による影響評価
口頭発表（一般）
- 第３０回 日本微生物生態学会, 2015年10月, 英語, 亀城プラザ 土浦市, 国内会議Ralfuranones secreted by Ralstonia solanacearum function as intercellular secondary messengers during its biofilm formation
ポスター発表
- 平成２７年度日本植物病理学会関西部会, 2015年09月, 日本語, あわぎんホール徳島県郷土文化会館, 国内会議ポリリン酸化合物処理による糸状菌病害防除法の開発
口頭発表（一般）
- 平成２７年度植物感染生理談話会, 2015年08月, 日本語, 松山市, 国内会議青枯病菌のＢＦ形成機構とその病原性への役割
[招待有り]
口頭発表（招待・特別）
- 平成２７年度植物感染生理談話会, 2015年08月, 日本語, 松山市, 国内会議糸状菌における不和合性反応を制御する遺伝子の解明
ポスター発表
- 平成２７年度植物感染生理談話会, 2015年08月, 日本語, 松山市, 国内会議いもち病菌の病原性発現過程におけるオートファジーの役割
ポスター発表
- 平成２７年度植物感染生理談話会, 2015年08月, 日本語, 松山市, 国内会議いもち病菌ゲノムのエピジェネティックな遺伝子制御
[招待有り]
口頭発表（招待・特別）
- 平成２７年度植物感染生理談話会, 2015年08月, 日本語, 松山市, 国内会議Ralstonia solanacearum OE1-1株のバイオフィルム形成にレクチンRS-IILは必要である
ポスター発表
- 日本植物病理学会大会, 2015年03月, 日本語, 明治大学, 国内会議青枯病菌のバイオフィルム形成への菌体外多糖の関与
口頭発表（一般）
- 日本植物病理学会大会, 2015年03月, 日本語, 明治大学, 国内会議次世代シーケンサを用いた白紋羽病菌の細胞質不和合性反応における発現変動遺伝子の解析
口頭発表（一般）
- 日本植物病理学会大会, 2015年03月, 日本語, 明治大学, 国内会議いもち病菌の根部感染過程にオートファジーは必須ではない．
口頭発表（一般）
- 日本植物病理学会大会, 2015年03月, 日本語, 明治大学, 国内会議MAPキナーゼPmk1およびMps1はH3K4ヒストンメチル基転移酵素MoSET1の上流で機能する．
口頭発表（一般）
- 日本植物病理学会大会, 2015年03月, 日本語, 明治大学, 国内会議furanone合成酵素をコードするralA遺伝子は青枯病菌のバイオフィルム形成に関与する．
口頭発表（一般）
- 糸状菌分子生物学コンファレンス, 2014年11月, 日本語, 仙台, 国内会議イネ科植物いもち病菌のArgonaute3はRNAサイレンシングを 負に制御する
ポスター発表
- 糸状菌分子生物学コンファレンス, 2014年11月, 日本語, 仙台, 国内会議MAPキナーゼPmk1はH3K4ヒストンメチル基転移酵素MoSET1の上流で機能する
ポスター発表
- 日本植物病理学会関西部会, 2014年09月, 日本語, 富山大学, 国内会議白紋羽病菌の和合性及び不和合性反応についての細胞学的・分子生物学的解析
口頭発表（一般）
- 日本植物病理学会関西部会, 2014年09月, 日本語, 国内会議The MAP kinase, Pmk1 is epistatic to MoSET1, H3K4 methyltransferase for infection-related morphogenesis in Magnaporthe oryzae
口頭発表（一般）
- IS-MPMI 2014 International Congress, 2014年07月, 英語, Rhodes, Greece, 国際会議Chromosomal Localization Patterns of Avirulence Effector Genes in the Rice Blast Fungus Pyricularia oryzae and Related Species
ポスター発表
- 日本植物病理学会全国大会, 2014年06月, 日本語, 札幌コンベンションセンター, 国内会議トマト植物の細胞間隙に接する細胞表面で青枯病菌はバイオフィルムを形成する
口頭発表（一般）
- 日本植物病理学会, 2014年06月, 日本語, 札幌, 国内会議MoSET1ヒストンメチルトランスフェラーゼに依存したいもち病菌の感染器官形成時の遺伝子発現
口頭発表（一般）
- 日本植物病理学会全国大会, 2014年06月, 日本語, 国内会議MoSET1ヒストンメチルカトランスフェラーゼに依存したいもち病菌の感染器官形成時の遺伝子発現
口頭発表（一般）
- 糸状菌分子生物学コンファレンス, 2013年11月, 日本語, 国内会議いもち病菌におけるヒストンリジンメチルトランスフェラーゼの役割
口頭発表（一般）
- Sino-Japan Joint Symposium of Plant Pathology, 2013年08月, 英語, Beijing, China, 国際会議Histone methyltransferase genes play roles in the pathogenicity of the rice blast fungus, Magnaporthe oryzae.
[招待有り]
口頭発表（招待・特別）
- 6th International Rice Blast Conference 2013, 2013年08月, 英語, 国際会議Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis.
口頭発表（一般）
- 日本植物病理学会関西部会, 2013年, 日本語, 国内会議植物病原糸状菌感染時において単子葉植物と双子葉植物で誘導されるパピラの形成機序に関する細胞学的比較解析
ポスター発表
- 日本土壌微生物学会, 2013年, 日本語, 国内会議糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析
ポスター発表
- 日本植物病理学会関西部会, 2013年, 日本語, 国内会議糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析
ポスター発表
- 日本植物病理学会関西部会, 2013年, 日本語, 国内会議リアルタイム定量PCR法による菌核病生物防除剤Coniothyrium minitansの追跡調査
ポスター発表
- 日本植物病理学会大会, 2013年, 日本語, 国内会議ヒストンメチル転移酵素群はイネ科植物いもち病菌の感染において重要な役割を果たす
口頭発表（一般）
- 日本植物病理学会大会, 2013年, 日本語, 国内会議ナシ黒斑病菌のRacAの病原性及び胞子発芽に及ぼす影響
口頭発表（一般）
- 日本植物病理学会大会, 2013年, 日本語, 国内会議いもち病菌の胞子発芽時におけるオートファジー誘導の経時的解析
口頭発表（一般）
- 6th International Rice Blast Conference 2013, 2013年, 英語, 国際会議Multiple host adhesion factors in Magnaporthe oryzae –potential target for disease control-
ポスター発表
- 日本植物病理学会関西部会, 2013年, 日本語, 国内会議MoSET1, histone methyltransferase responsible for methylation of histone H3 lysine 4 in Magnaporthe oryzae is required for both activation of gene expression during infection-related morphogenesis of the fungus
口頭発表（一般）
- 6th International Rice Blast Conference 2013, 2013年, 英語, 国際会議Macroautophagy during appressorium morphogenesis in Magnaporthe oryzae
ポスター発表
- 10th International Congress of Plant Pathology, 2013年, 英語, 国際会議Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis
[招待有り]
口頭発表（招待・特別）
- 10th International Congress of Plant Pathology, 2013年, 英語, 国際会議Autophagy-inducing condition during infection process of Magnaporthe oryzae
ポスター発表
- 10th International Congress of Plant Pathology, 2013年, 英語, 国際会議Attachment of airborne pathogens to their host; a potential target for disease control
[招待有り]
口頭発表（招待・特別）
- IS-MPMI 2012 International Congress, 2012年07月, 英語, Kyoto, Japan, 国際会議Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species.
口頭発表（一般）
- The 2nd Korea-Japan Joint Symposium 2012, 2012年03月, 英語, 国際会議Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species
ポスター発表
- 日本植物病理学会関西部会, 2012年, 日本語, 国内会議ナシ黒斑病菌の付着器局在性NADPH oxidase Bは侵略力発揮に必須である
口頭発表（一般）
- 平成24年度日本植物病理学会大会, 2012年, 日本語, 国内会議いもち病菌比病原力遺伝子のMultiple translocationはなぜ起こったか―欠失／再獲得仮説
口頭発表（一般）
- 平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議細胞壁加水分解酵素群はイネ科植物いもち病菌による角皮侵入に寄与している
口頭発表（一般）
- 平成23年度日本植物病理学会大会, 2011年, 日本語, 国内会議いもち病菌の非病原力遺伝子座Avr1-CO39における機能分子の同定
口頭発表（一般）
- 平成23年度日本植物病理学会関西部会, 2011年, 日本語, 国内会議Building Blocks法によるイネ科植物いもち病菌細胞壁加水分解酵素群の機能解析
口頭発表（一般）
- ムギ類研究会, 2010年11月, 日本語, 帯広, 国内会議イネ科植物いもち病菌集団における非病原力遺伝子AVR-Pitaの染色体彷徨
口頭発表（一般）
- 日本植物病理学会大会, 2010年, 日本語, 国内会議ハイドロフォビンMhp1のいもち病菌宿主接着に及ぼす影響
ポスター発表
- The 4th CeFP International Symposium on Fungal Biology and Pathogenesis, 2010年, 英語, Seoul National University, Korea, 国際会議RNA silencing as a novel tool for uncovering gene function in fungi
[招待有り]
口頭発表（招待・特別）
- 平成21年度日本植物病理学会大会, 2009年, 日本語, 国内会議冠さび病菌感染エンバク葉に誘導されるアベナスラミド蓄積とその生合成遺伝子発現の局在と配向解析
口頭発表（一般）
- 平成21年度日本植物病理学会関西部会, 2009年, 日本語, 国内会議いもち病菌小分生子の細胞学的特徴
口頭発表（一般）
- 日本植物病理学会大会, 2009年, 日本語, 国内会議いもち病菌におけるハイドロフォビンの接着性と病原性に及ぼす影響
ポスター発表
- 6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009年, 英語, 国際会議Pathological roles of reactive oxygen species in plant pathogen
口頭発表（一般）
- 日本植物病理学会大会, 2008年, 日本語, 国内会議ナシ黒斑病菌における活性酸素種 (reactive oxygen species: ROS)生成遺伝子noxAおよびnoxB変異株の細胞学・病理学的比較調査
口頭発表（一般）
- 第8回糸状菌分子生物学コンファレンス, 2008年, 日本語, 国内会議いもち病菌におけるハイドロフォビン(Mpg1)の接着能力への影響
ポスター発表
- 第1回神戸大学バイオサイエンス研究会・若手研究者交流会, 2008年, 日本語, 国内会議Pathological roles of reactive oxygen species at infection structures of Alternaria alternata Japanese pear pathotype during plant infection
ポスター発表
- 日本植物病理学会関西部会, 2007年, 日本語, 国内会議ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）生成遺伝子NoxBの機能解析
口頭発表（一般）
- 日本植物病理学会, 2007年, 日本語, 国内会議ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）生成遺伝子NoxAの機能解析
口頭発表（一般）
- 日本植物病理学会, 2007年, 日本語, 国内会議いもち病菌小型分生子の病理学的役割を解明するための基礎調査
口頭発表（一般）
- 日本植物病理学会関西部会, 2007年, 日本語, 国内会議いもち病菌のシトシンDNAメチル化は同菌の生活環において必須ではない
口頭発表（一般）
- 第6回糸状菌分子生物学コンファレンス, 2006年, 日本語, 国内会議ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）の機能解析
ポスター発表
- Plant & Animal Genome XIV Conference. WorkShop AbstactP20., 2006年, 英語, 未記入, San Diego, 国際会議Applications of RNA silencing in Magnaporthe oryzae.
その他
- 平成17年日本植物病理学会関西部会講演要旨集p29, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議比較マッピングによるいもち病菌の染色体構造解析
口頭発表（一般）
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 199）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議植物病原糸状菌Magnaporthe oryzaeにおけるRNAi経路には二つのDicer 様タンパク質のうち一つが主に関与する
口頭発表（一般）
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 299-300）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議トマト転写因子Pti5 遺伝子のプロモーター配列解析
口頭発表（一般）
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 300）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議トマト転写因子Pti4, 5, 6 によるPti5 プロモーターの複合的な制御
口頭発表（一般）
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 198-199）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議シコクビエいもち病菌集団における非病原力遺伝子PWL1 の解析
口頭発表（一般）
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 199）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議コムギのエンバクいもち病菌に対する抵抗性遺伝子Rwt4の野外における役割
口頭発表（一般）
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 198）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議いもち病菌集団における非病原力遺伝子Avr-Pita 座の変動機構
口頭発表（一般）
- 平成17年日本植物病理学会関西部会講演要旨集p30, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議いもち病菌の種特異的寄生性に関するPWT3 に対応する抵抗性遺伝子の検出とgene-forgene関係の証明
口頭発表（一般）
- 平成17年日本植物病理学会関西部会講演要旨集p30, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定
口頭発表（一般）
- 日本植物病理学会関西部会, 2005年, 日本語, 国内会議イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定
口頭発表（一般）
- 平成17年日本植物病理学会関西部会講演要旨集p29, 2005年, 日本語, 日本植物病理学会, 名古屋市, 国内会議アワいもち病菌のイチゴツナギ亜科植物に対する非病原性の比較解析
口頭発表（一般）
- XXIII Fungal Genetics Conference. Abstact p163., 2005年, 英語, 未記入, California, 国際会議RNA silencing as a tool for exploring gene function in Ascomycete fungi.
その他
- 平成17年日本植物病理学会大会 （日本植物病理学会報，71: 198）, 2005年, 日本語, 日本植物病理学会, 静岡, 国内会議Pyricularia oryzae 近縁種の種分化過程のGCPSRによる解析
口頭発表（一般）
- XXIII Fungal Genetics Conference. Abstact p159., 2005年, 英語, 未記入, California, 国際会議One of the two Dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related siRNA accumulation.
その他
- 第7回RNA学会ミーティング（講演要旨集p86), 2005年, 日本語, 日本RNA学会, 弘前大学, 国内会議DNA methyltransferase は転移因子由来siRNAの蓄積を促進する
口頭発表（一般）
- 第7回RNA学会, 2005年, 日本語, 弘前大学, 国内会議DNA methyltransferaseは転移因子由来siRNAの蓄積を促進する
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議様々な植物から分離したPyricularia属菌におけるGCPSRに基づいた種の識別
口頭発表（一般）
- 第6回RNAミーティング, 2004年, 日本語, RNAミーティング, 国内会議植物病原糸状菌Magnaporthe oryzaeにおけるRNAi経路には二つのDicer様タンパク質のうち一つが主に関与する
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議セントピートグラスに発生したいもち病菌のrDNA-ITS2領域とbeta-tubulin遺伝子の解析における病原性発現物質の解析
口頭発表（一般）
- 平成16年日本植物病理学会関西部会, 2004年, 日本語, 日本植物病理学会, 松山, 国内会議シコクビエいもち病菌(Magnaporthe oryzae)の集団構造の解析
口頭発表（一般）
- 平成16年日本植物病理学会関西部会, 2004年, 日本語, 日本植物病理学会, 松山, 国内会議コムギ品種とエンバクいもち病菌間における遺伝子対遺伝子関係の証明
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議エンバクの抵抗性誘導におけるアポトーシス機構の解明14）エンバクにおける宿主特異的毒素ビクトリンへの結合因子のビアコアによる探索
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議エンバクの抵抗性誘導におけるアポトーシス機構の解明13）宿主特異的毒素ビクトリンは感受性品種IowaX469において細胞表面で認識される
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議いもち病菌集団における染色体構造の多様性と非病原性遺伝子座の変動
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議いもち病菌Magnaporthe oryzaeにおけるRNAサイレンシングベクターの作製
口頭発表（一般）
- 平成16年日本植物病理学会関西部会, 2004年, 英語, 日本植物病理学会, 松山, 国内会議Degradation of ribosomal RNA associated with apoptotic response of oat cells trigered by the host-selective toxin, victorin.
口頭発表（一般）
- 平成16年日本植物病理学会大会, 2004年, 日本語, 日本植物病理学会, 福岡, 国内会議Arabidopsisにおける植物成長促進根圏微生物（PGPR)の遺伝子変動のマイクロアレイ解析
口頭発表（一般）
- 平成16年日本植物病理学会関西部会, 2004年, 英語, 日本植物病理学会, 松山, 国内会議A convergent opposing promoters system to explore genome-wide gene function in the rice blast fungus by an RNA silencing approach.
口頭発表（一般）
- 第3回糸状菌分子生物学コンファレンス, 講演要旨集, 2003年11月, 日本語, 糸状菌分子生物学研究会, 東京大学, 国内会議いもち病菌Magnaporthe oryzae におけるRNA サイレンシング
口頭発表（一般）
- 第3回糸状菌分子生物学コンファレンス, 講演要旨集, 2003年11月, 日本語, 糸状菌分子生物学研究会, 東京大学, 国内会議Pyricularia 属集団における染色体構造の多様性
口頭発表（一般）
- 第75回日本遺伝大会・ワークショップ講演, 講演要旨集, 2003年09月, 日本語, 日本遺伝学会, 東北大学, 国内会議いもち病菌集団における染色体構造の多様性
口頭発表（一般）
- 第75回日本遺伝大会, 講演要旨集, 2003年09月, 日本語, 日本遺伝大会, 東北大学, 国内会議いもち病菌における過剰染色体の遺伝様式と染色体構造変異の機構
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議ペレニアルライグラスいもち病菌の地理的機嫌の解明とコムギに対する発病ポテンシャルの評価
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議ナス科植物青枯病生物防除因子Pseudomonas fluorescens のNOR遺伝子破壊による改変
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議いもち病菌集団における染色体構造の多様性
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議いもち病菌の種特異的寄生性機構に関する研究(13) いもち病菌雑種後代で認められるadult resistance に対する病原性の低下について
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議いもち病菌における病原性発現物質の解析7)エンバクいもち病菌の生産する新規毒素の白化,活性酸素生成, 細胞死の誘導
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議いもち病菌におけるsupernumerary chromosome の遺伝様式と構造変異機構
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議GCPSR に基づいたMagnaporthe/Pyricularia 属菌集団における種の識別
口頭発表（一般）
- 平成15年日本植物病理学会大会，日本植物病理学会報, 2003年, 日本語, 日本植物病理学会, 未記入, 国内会議いもち病菌Magnaporthe grisea におけるsmall interfering RNAs (siRNAs)の検出
口頭発表（一般）
- Plant & Animal Genome XI Conference., 2003年, 英語, 未記入, 未記入, 国際会議Mechanisms of species-specific parasitism in Magnaporthe oryzae.
口頭発表（一般）
- 9th US-Japan Seminar on Plant-pathogen Interactions., 2003年, 英語, 未記入, 未記入, 国際会議Apoptotic response of oat leaf cells in defense against infection.
口頭発表（一般）
所属学協会
共同研究・競争的資金等の研究課題
- 学術研究助成基金助成金／挑戦的研究（萌芽）, 2017年04月 - 2019年03月, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2016年04月 - 2019年03月, 研究代表者
競争的資金
- 科学研究費一部基金／基盤研究(B)特設, 2013年04月 - 2016年03月, 研究代表者
競争的資金
- 戦略的イノベーション創造プログラム（SIP）, 2016年, 研究代表者持続可能な農業生産のための新たな総合的植物保護技術の開発
競争的資金
- 科学研究費補助金／萌芽研究, 2012年04月 - 2014年03月, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2010年, 研究代表者
競争的資金
- 特別研究員奨励費, 2008年, 研究代表者
競争的資金
- 科学研究費補助金／萌芽研究, 2007年, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2006年
競争的資金
- 科学研究費補助金／基盤研究(B), 2005年, 研究代表者
競争的資金
- 科学研究費補助金／特定領域研究, 2005年, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2005年
競争的資金