研究者紹介システム
| 大山 憲二 |  |
| オオヤマ ケンジ | |
| 大学院農学研究科 附属食資源教育研究センター | |
| 教授 | |
| 獣医学畜産学関係 |
研究者情報
所属
【主配置】
大学院農学研究科 附属食資源教育研究センター【配置】
農学部 資源生命科学科, 大学院農学研究科 資源生命科学専攻
学位
研究シーズ
研究シーズ紹介サイトへのリンク授業科目
- 特定課題演習 I-1, 大学院農学研究科, 2021
- 課題開発演習, 大学院農学研究科, 2021
- 資源生命科学プレゼンテーション演習Ⅰ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-1, 大学院農学研究科, 2021
- 神戸大学発次世代農資源生産学, 大学院農学研究科, 2021
- 特定課題演習 I-2, 大学院農学研究科, 2021
- 資源生命科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 有用動物資源学, 大学院農学研究科, 2021
- 先端遺伝育種論, 大学院農学研究科, 2021
- Global Topics in Animal Science, 農学部, 2021
- 高度教養セミナー農学部応用動物学, 農学部, 2021
- 卒業研究, 農学部, 2021
- 農場実習（生産環境工学コース）, 農学部, 2021
- 資源生命科学入門Ⅰ－１, 農学部, 2021
- 牧場実習, 農学部, 2021
- 量的遺伝学１, 農学部, 2021
- 農場実習II, 農学部, 2021
- 農場実習（応用生命化学コース）, 農学部, 2021
- 農場実習（環境生物学コース）, 農学部, 2021
- 初年次セミナー（応用動物学コース）, 農学部, 2021
- 農場と食卓をつなぐフィ－ルド演習, 農学部, 2021
- 資源生命科学入門Ⅰ－２, 農学部, 2021
- 量的遺伝学２, 農学部, 2021
- 高度教養セミナー農学部応用動物学入門, 農学部, 2021
- 高度教養セミナー農学部応用動物学入門, 農学部, 2021
- 応用動物学各論, 農学部, 2021
- 外国書講読 I, 農学部, 2021
- 外国書講読Ｂ, 農学部, 2021
- 食の倫理, 農学部, 2021
- 食の倫理の探究, 農学部, 2021
- 応用動物学演習, 農学部, 2021
ジャンル
コメントテーマ
研究活動
研究キーワード
研究分野
委員歴
- 2021年04月, 独立行政法人家畜改良センター, 肉用牛改良専門委員会（委員長）
- 2021年04月, 一般社団法人家畜改良事業団, 肉用牛産肉能力平準化促進事業に係る改良委員会（委員長）
- 肉用牛研究会, 評議員
- 日本動物遺伝育種学会, 理事
- 日本畜産学会, 編集委員セクションエディター
- 農林水産省, 食料・農業・農村政策審議会畜産部会
- 農林水産省, 家畜改良増殖目標畜種別研究会（肉用牛）（座長）
- 農林水産省, 和牛遺伝資源の知的財産的価値の保護強化に関する専門部会
- 農林水産省, 和牛遺伝資源の流通管理に関する有識者検討会委員
- 農林水産省, 肉用子牛生産者補給金に係る算定方式検討会（座長）
- 兵庫県, 農林水産政策審議会
- 鳥取県和牛改良委員会
- 兵庫県肉用牛改良委員会（委員長）
- 公益社団法人全国和牛登録協会, 中央審査委員（委員長）
- 公益社団法人全国和牛登録協会, 産肉能力検定委員（委員長）
- 公益社団法人全国和牛登録協会, 育種推進委員（副委員長）
受賞
2019年11月 肉用牛研究会, 肉用牛研究会賞
2019年10月 神戸大学, 学長表彰（財務貢献者）
2018年09月 日本動物遺伝育種学会, 日本動物遺伝育種学会優秀発表賞, 兵庫県黒毛和種における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出
日本国国内学会・会議・シンポジウム等の賞
2018年03月 日本畜産学会, 第124回日本畜産学会大会優秀発表賞, ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
日本国国内学会・会議・シンポジウム等の賞
2017年09月 一般社団法人産業応用工学会, Best Paper Award, Estimation of Calf Weight from Fixed-Point Stereo Camera Images Using Three-Dimensional Successive Cylindrical Model
国際学会・会議・シンポジウム等の賞
2016年11月 日本動物遺伝育種学会, 第17回日本動物遺伝育種学会大会・学会長特別賞, 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連
日本国国内学会・会議・シンポジウム等の賞
2016年08月 Secretariat for AAAP2016, 17th AAAP Animal Science Congress Young Scientists Award, Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima
日本国国際学会・会議・シンポジウム等の賞
2015年03月 Japanese Society of Animal Science, Animal Science Journal Excellent Paper award, Effect of DNA polymorphisms related to fatty acid composition in adipose tissue
日本国国内学会・会議・シンポジウム等の賞
2013年03月 Japanese Society of Animal Science, Animal Science Journal Excellent paper award, Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds
国内学会・会議・シンポジウム等の賞
論文
In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.
MDPI AG, 2022年07月01日, Genes, 13 (7), 1190 - 1190研究論文（学術雑誌）
Genetic parameters for carcass grading traits, image analysis traits, and monounsaturated fatty acid (MUFA) percentages were estimated in 29,942 Japanese Black cattle from Hyogo Prefecture. The analyzed traits included five carcass grading traits, two image analysis traits, fat area ratio and fineness index, and two MUFA traits, one measured in intermuscular fat using near-infrared spectroscopy (NIRS) and the other in intramuscular fat using gas chromatography (GC). The heritability estimates of image analysis traits and MUFA were moderate to high, ranging from 0.395 to 0.740, and it was considered that they could be improved simultaneously with carcass grading traits because no severe genetic antagonism was observed. Although the heritability of the NIRS-based intermuscular MUFA was slightly lower than that of the GC-based intramuscular MUFA, the genetic correlation between the two methods was as high as 0.804. These results indicate that the NIRS method can be used as an alternative evaluation procedure to predict MUFA in intramuscular fat in the longissimus muscle.
Wiley, 2021年12月, Animal Science Journal, 92 (1), e13664, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- MDPI, 2021年11月25日, The 13th EFITA International Conference
研究論文（国際会議プロシーディングス）
- Japanese Society of Animal Science, 2021年11月25日, 日本畜産学会報, 92 (4), 511 - 517, 日本語
[査読有り]
研究論文（学術雑誌）
Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.
MDPI AG, 2021年06月22日, Life, 11 (7), 597 - 597研究論文（学術雑誌）
- IEEE, 2021年01月09日, 2021 IEEE 18th Annual Consumer Communications & Networking Conference (CCNC)
[査読有り]
研究論文（国際会議プロシーディングス）
Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.
Copernicus GmbH, 2020年01月13日, Archives Animal Breeding, 63 (1), 9 - 17, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- Wiley, 2020年01月, Animal Science Journal, 91 (1), e13335
[査読有り]
研究論文（学術雑誌）
- 2020年01月, Animal Science Journal, 91, e13338Estimation of genetic parameters of defective appearances in Japanese Black heifer calves in Kagoshima
[査読有り]
研究論文（学術雑誌）
Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.
2019年12月, Heliyon, 5 (12), e03006, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- 2019年11月, 日本畜産学会報, 90 (4), 337 - 340, 日本語但馬牛における致死相当量の推定
[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2019年06月, Meta Gene, 20, 100558 - 100558, 英語
[査読有り]
研究論文（学術雑誌）
- 2019年06月, 12th European Federation for Information Technology in Agriculture, Food and the Environment (EFITA) International Conference, 英語Image Extraction Based on Depth Information for Calf Body Weight Estimation
[査読有り]
研究論文（国際会議プロシーディングス）
- 2019年06月, 12th European Federation for Information Technology in Agriculture, Food and the Environment (EFITA) International Conference, 英語Detection of Approaching Interaction with Cattle in Estrus Based on Community Transition and Cattle Distance
[査読有り]
研究論文（国際会議プロシーディングス）
- Wiley, 2019年04月, Animal Science Journal, 90 (4), 467 - 472, 英語
[査読有り]
研究論文（学術雑誌）
- (公社)日本畜産学会, 2019年03月, 日本畜産学会大会講演要旨集, 125回, 177 - 177, 日本語複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
- Institute of Electronics, Information and Communications Engineers ({IEICE}), 2019年, IEICE Communications Express
[査読有り]
- Institute of Electronics, Information and Communications Engineers ({IEICE}), 2019年, IEICE Communications Express
[査読有り]
- Japanese Society of Animal Breeding and Genetics, 2019年, The Journal of Animal Genetics, 47 (2), 37 - 45
[査読有り]
研究論文（学術雑誌）
- 2018年12月, Proceedings of the 9th International Symposium on Information and Communication Technology, 243 - 250, 英語Refined Cattle Detection Using Composite Background Subtraction and Brightness Intensity from Bird’s Eye Images
[査読有り]
研究論文（国際会議プロシーディングス）
- 2018年12月, Proceedings of the 9th International Symposium on Information and Communication Technology, 235 - 242, 英語Quantifying the Approaching Behaviors for Interactions in Detecting Estrus of Breeding Cattle
[査読有り]
研究論文（国際会議プロシーディングス）
- 2018年12月, Proceedings of the 9th International Symposium on Information and Communication Technology, 227 - 234, 英語Cattle Community Extraction Using the Interactions Based on Synchronous Behavior
[査読有り]
研究論文（国際会議プロシーディングス）
- 2018年12月, Proceedings of the 9th International Symposium on Information and Communication Technology, 208 - 215, 英語Calf Robust Weight Estimation Using 3D Contiguous Cylindrical Model and Directional Orientation from Stereo Images
[査読有り]
研究論文（国際会議プロシーディングス）
- Wiley, 2018年08月, Animal Science Journal, 89 (8), 1060 - 1066, 英語
[査読有り]
研究論文（学術雑誌）
- Wiley, 2018年05月, Animal Science Journal, 89 (5), 743 - 751, 英語
[査読有り]
研究論文（学術雑誌）
- (公社)日本畜産学会, 2018年03月, 日本畜産学会大会講演要旨集, 124回, 111 - 111, 日本語ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
Various studies have been conducted on methods for estimating the weight of cattle. In this study, we propose a method to estimate body weight by modeling the shape of calf using three-dimensional information extracted from the stereo images. Initially, a stereo camera is set with two fixed network cameras, to take a motion image of a calf. Three-dimensional coordinate is calcu
産業応用工学会, 2018年01月, Journal of the Institute of Industrial Applications Engineers (JIIAE), 6 (1), 39 - 46, 英語[査読有り]
研究論文（学術雑誌）
- Genetics and Molecular Research, 2018年, Genetics and Molecular Research, 17 (3), 18056, 英語
[査読有り]
研究論文（学術雑誌）
黒毛和種の損徴は品種の特徴を損なうだけでなく，経済的損失となり得るものもある．本研究では，損徴発生率の低減に関する知見を得ることを目的に，1999年～2015年の鹿児島県産黒毛和種雌子牛511,337頭の記録を用いて，白斑，舌の異常，乳頭の異常，被毛の異常について基礎的な調査を試みた．全データにおける損徴発生率は被毛の異常の0.29%から白斑の3.68%の範囲であり，年次推移はすべて増加傾向を示した．白斑の発生部位も年次により変化した．白斑では，近交度が高まると発生率が有意に上昇した（P<0.05）．被毛の損徴以外では，母牛の損徴の有無が子牛の損徴発生率に有意に影響した（P<0.01）．さらに，白斑と舌の異常においては，損徴を持つ母牛と持たない母牛の子牛の損徴発生率を種雄牛別に算出したところ両者に高い正の相関が得られ，両親の組合せを考慮することで損徴の発生を低減できる可能性を示した．
公益社団法人 日本畜産学会, 2017年11月, 日本畜産学会報, 88 (4), 425 - 430, 日本語[査読有り]
研究論文（学術雑誌）
- 2017年09月, Animal Veterinary Sciences, 5, 69 - 72, 英語
[査読有り]
研究論文（学術雑誌）
Various studies have been conducted on methods for estimating the weight of cattle. In this study, we propose a method to estimate body weight by modeling the shape of calf using three-dimensional information extracted from the stereo images. Initially, a stereo camera is set with two fixed network cameras, to take a motion image of a calf. Three-dimensional coordinate is calcu
産業応用工学会, 2017年07月, The 5th IIAE International Conference on Intelligent Systems and Image Processing 2017 (ICISIP2017), 247 - 254, 英語[査読有り]
研究論文（国際会議プロシーディングス）
Breeding cows are known to engage in social activities, such as interacting with other cows and forming groups similarly to humans. This paper proposes a method to extract information of interaction between cows, regarding it as a primary element of sociality. In order to extract such information, we record bird's eye pictures in a pasture by a fixed point camera set on a silo
産業応用工学会, 2017年07月, The 5th IIAE International Conference on Intelligent Systems and Image Processing 2017 (ICISIP2017), 239 - 246, 英語[査読有り]
研究論文（国際会議プロシーディングス）
Previous studies have indicated that some leptin gene polymorphisms were associated with economically important traits in cattle breeds. However, polymorphisms in the leptin gene have not been reported thus far in Japanese Black cattle. Here, we aimed to identify the leptin gene polymorphisms which are associated with carcass traits and fatty acid composition in Japanese Black cattle. We sequenced the full-length coding sequence of leptin gene for eight Japanese Black cattle. Sequence comparison revealed eight single nucleotide polymorphisms (SNPs). Three of these were predicted to cause amino acid substitutions: Y7F, R25C and A80V. Then, we genotyped these SNPs in two populations (JB1 with 560 animals and JB2 with 450 animals) and investigated the effects on the traits. Y7F in JB1 and A80V in JB2 were excluded from statistical analysis because the minor allele frequencies were low (<0.1). Association analysis revealed that Y7F had a significant effect on the dressed carcass weight in JB2; R25C had a significant effect on C18:0 and C14:1 in JB1 and JB2, respectively; and A80V had a significant effect on C16:0, C16:1, C18:1, monounsaturated fatty acid and saturated fatty acid in JB1. The results suggested that these SNPs could be used as an effective marker for the improvement of Japanese Black cattle.
WILEY, 2017年03月, ANIMAL SCIENCE JOURNAL, 88 (3), 433 - 438, 英語[査読有り]
研究論文（学術雑誌）
Genetic parameters were estimated to investigate the relationships between meat quality traits and fatty acid composition from 11855 Japanese Black cattle. The meat quality traits included beef marbling score (BMS), beef color score, firmness of beef (FIR), texture of beef (TEX) and beef fat color score (BFS). The data on fatty acid composition included oleic acid and monounsaturated fatty acids (MUFA) contents, the ratio of MUFA to saturated fatty acids (MUS) and the ratio of elongation. The heritability estimates for meat quality traits ranged from moderate (0.30) to high (0.72). The strong genetic correlations between them were useful for simultaneous genetic improvement. In addition, high heritability estimates of fatty acid composition, ranging from 0.60 to 0.63, indicated that they could also be improved genetically. The genetic correlations of fatty acid composition with BMS, FIR and TEX were weak and negative. In contrast, positive and stronger genetic correlations were found between BFS and fatty acid composition, in particular, related to the level of unsaturation (0.77 and 0.79 for MUFA and MUS, respectively). This implies that improving the level of unsaturation makes fat darker (more yellow) and thus requires balancing with BFS.
WILEY-BLACKWELL, 2017年01月, ANIMAL SCIENCE JOURNAL, 88 (1), 11 - 18, 英語[査読有り]
研究論文（学術雑誌）
- Japanese Society of Animal Breeding and Genetics, 2017年, 動物遺伝育種研究, 45 (1), 3 - 8, 日本語
[査読有り]
研究論文（学術雑誌）
Meat quality is one of the most important traits determining carcass price in the Japanese beef market. Optimized breeding goals and management practices for the improvement of meat quality traits requires knowledge regarding any potential functional relationships between them. In this context, the objective of this research was to infer phenotypic causal networks involving beef marbling score (BMS), beef color score (BCL), firmness of beef (FIR), texture of beef (TEX), beef fat color score (BFS), and the ratio of MUFA to SFA (MUS) from 11,855 Japanese Black cattle. The inductive causation (IC) algorithm was implemented to search for causal links among these traits and was conditionally applied to their joint distribution on genetic effects. This information was obtained from the posterior distribution of the residual (co)variance matrix of a standard Bayesian multiple trait model (MTM). Apart from BFS, the IC algorithm implemented with 95% highest posterior density (HPD) intervals detected only undirected links among the traits. However, as a result of the application of 80% HPD intervals, more links were recovered and the undirected links were changed into directed ones, except between FIR and TEX. Therefore, 2 competing causal networks resulting from the IC algorithm, with either the arrow FIR -> TEX or the arrow FIR -> TEX, were fitted using a structural equation model () to infer causal structure coefficients between the selected traits. Results indicated similar genetic and residual variances as well as genetic correlation estimates from both structural equation models. The genetic variances in BMS, FIR, and TEX from the structural equation models were smaller than those obtained from the MTM. In contrast, the variances in BCL, BFS, and MUS, which were not conditioned on any of the other traits in the causal structures, had no significant differences between the structural equation model and MTM. The structural coefficient for the path from MUS (BCL) to BMS showed that a 1-unit improvement in MUS (BCL) resulted in an increase of 0.85 or 1.45 (an decrease of 0.52 or 0.54) in BMS in the causal structures. The analysis revealed some interesting functional relationships, direct genetic effects, and the magnitude of the causal effects between these traits, for example, indicating that BMS would be affected by interventions on MUS and BCL. In addition, if interventions existed in this scenario, a breeding strategy based only on the MTM would lead to a mistaken selection for BMS.
AMER SOC ANIMAL SCIENCE, 2016年10月, JOURNAL OF ANIMAL SCIENCE, 94 (10), 4133 - 4142, 英語[査読有り]
研究論文（学術雑誌）
Cattle exhibit a range of carcass defects, including blood splash (BLS), intramuscular edema (INE), muscle steatosis (MUS), bruising (BR), trim loss (TRL) and others (OTH). These defects lower the carcass value and can result in significant economic loss to producers. We estimated the incidence, relationship with inbreeding coefficients and genetic parameters of carcass defects in Japanese Black cattle using 561 619 carcass records from Kagoshima, Japan during April 1988 through March 2011. The defect incidence ranged from 0.22% for TRL to 5.73% for BR. The incidence of MUS and BR increased from 1.21% to 6.57% and from 1.06% to 9.31%, respectively. The incidence of INE peaked at 7.44% in 1999 and decreased thereafter. We observed a positive linear relationship between the defect incidence and the inbreeding coefficients in MUS, BR and TRL (P<0.01). The heritabilities estimated by univariate animal model with Gibbs sampling for BLS, INE, MUS, BR and TRL were 0.24, 0.06, 0.18, 0.05 and 0.02, respectively. The contribution of farm variance to phenotypic variance was negligible (0.01 to 0.04). Significant genetic correlations of TRL were estimated with MUS (0.63) and BR (0.63). Our results suggest that genetic factors contribute to the incidence of BLS and MUS.
WILEY-BLACKWELL, 2016年05月, ANIMAL SCIENCE JOURNAL, 87 (5), 655 - 660, 英語[査読有り]
研究論文（学術雑誌）
The objective of this study was to estimate the direct economic losses due to the condemnation of the liver and large intestine because of internal diseases (multifocal necrosis in the liver (MNL) and inflammation of the large intestine (ILI)), and the indirect losses because of reductions in carcass performance from MNL, bovine abdominal fat necrosis (BFN) and ILI using data from 5383 Japanese Black cattle. Direct losses were estimated by multiplying the price of the condemned part by the frequency of its occurrence owing to the disease. Similarly, indirect losses were estimated as the product of unit carcass price and reduction in carcass weight (CW) due to the disease. The direct impact on the beef cattle industry from MNL and ILI was estimated at around $1.29 million (US$1 = (sic)120) per year. A least-squares analysis showed that MNL had no influence on any carcass trait, whereas BFN and ILI significantly reduced CW, rib eye area and darkened the beef. ILI also reduced rib thickness. The indirect losses from BFN and ILI were estimated as a maximum of $131.7 and $256.4 per animal and around $6.26 million and $4.03 million for the industry, respectively, mostly because of the reduction in CW.
WILEY-BLACKWELL, 2016年05月, ANIMAL SCIENCE JOURNAL, 87 (5), 736 - 741, 英語[査読有り]
研究論文（学術雑誌）
Consideration of the shortened fattening period seems to be worthwhile for the realization of profitable beef production. In this study, change of fatty acid composition of the lumbar longissimus during the final stage of fattening was investigated in Japanese Black cattle. Each of 110 fattening animals was sampled three times: the initial two samples were taken by biopsy (25.7 months and 27.5 months on average) and the final one was from carcasses (29.9 months on average). Preliminary analysis indicated that removing muscle tissues from the constant body position of the living animals should be essential for sampling. Average monounsaturated fatty acids (MUFA) at three sampling points were 58.1%, 58.5% and 60.5%, and the differences of the third sampling with the first and second samplings were significant. Both in steers and heifers, MUFA also increased as the fattening stage proceeded, and MUFA of the heifers at all the sampling points were significantly higher than those of the steers. The increasing rate of MUFA rose from 0.21 percentage points (pp)/month at period 1 (from the first sampling to the second sampling) to 0.84 pp/month at period 2 (from the second sampling to the slaughter).
WILEY-BLACKWELL, 2016年04月, ANIMAL SCIENCE JOURNAL, 87 (4), 578 - 583, 英語[査読有り]
研究論文（学術雑誌）
- Extraction of Interaction Information Between Breeding Cows with GPS and Its Application to Estrus Detection
This study focuses on bovine sociality and proposes a new method of detecting estrus through the detection and the analysis of interactions between cows. In the proposed method, we mainly trace bovine behavior when an animal approaches other cows based on their time-series location data measured by a GPS installed on each animal. The cow's estrus is detected by analyzing the interaction information extracted from the behavior. Our proposed method experimentally obtained more accurate results than the existing method of estrus detection.
IEEE, 2016年, 2016 INTERNATIONAL JOINT CONFERENCE ON NEURAL NETWORKS (IJCNN), 2967 - 2972, 英語[査読有り]
研究論文（国際会議プロシーディングス）
- A Method of Detecting Interaction Between Breeding Cows from Time-series Bird's Eye Pictures of Pasture
Breeding cows are known to engage in sociality, in which they interact and form groups. This paper proposes a method of detecting the interaction between breeding cows from time-series pictures of pastures by a similar image retrieval method using a Bag of Visual Words. We divided the interaction detection into three tasks: detecting a pair of cows in an interaction, pinpointing the time and the place that the interaction was observed and identifying its type. This paper focuses on the identifying task. In the proposed method, a similar image retrieval method is used to classify the interaction, and the information from the states of preceding and succeeding the interaction is considered to improve the accuracy of the interaction classification. The effectiveness of the proposed method was confirmed experimentally.
IEEE, 2016年, 2016 INTERNATIONAL JOINT CONFERENCE ON NEURAL NETWORKS (IJCNN), 2973 - 2978, 英語[査読有り]
研究論文（国際会議プロシーディングス）
- Japanese Society of Animal Science, 2016年, 日本畜産学会報, 87 (4), 333 - 338, 日本語
[査読有り]
研究論文（学術雑誌）
The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.
WILEY-BLACKWELL, 2015年08月, ANIMAL SCIENCE JOURNAL, 86 (8), 737 - 746, 英語[査読有り]
研究論文（学術雑誌）
- 2015年05月, 電気学会 第62回情報システム研究会, IS-15-009, 日本語放牧場の俯瞰動画データを用いた繁殖牛のインタラクション検出
研究論文（研究会，シンポジウム資料等）
- 2015年05月, 電気学会 第62回情報システム研究会, IS-15-001, 日本語GPSを利用した放牧牛のインタラクション情報の取得とその分析
研究論文（研究会，シンポジウム資料等）
- 2015年05月, Journal of Animal Science, 93 (6), 2714 - 2721, 英語Genetic relationships between internal diseases diagnosed at slaughter and carcass traits in Japanese Black cattle
[査読有り]
研究論文（学術雑誌）
- 2014年08月, Proceedings of 10th World Congress on Genetics Applied to Livestock Production, 749, 英語Searching for phenotypic causal links among meat quality traits in Japanese Black cattle
[査読有り]
研究論文（国際会議プロシーディングス）
Fatty acid composition of beef adipose tissue is one of its important traits because a high proportion of monounsaturated fatty acid is related to favorable beef flavor and tenderness. In this study, we searched polymorphisms in full length coding DNA sequence of urotensin 2 recepter and investigated the effects on fatty acid composition (C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, monounsaturated fatty acid, saturated fatty acid). Eight single nucleotide polymorphisms (SNP) were identified by sequence comparison among eight animals, including five Japanese Black and three Holstein cattle. One of these SNP (c.866C>T) was predicted to cause amino acid substitutions (P289L) and the other seven synonymous SNP, including c.267C>T, were presumed to be in linkage disequilibrium. Therefore we selected two SNP (c.267C>T and c.866C>T) for further analysis. We investigated associations between these genotypes and fatty acid composition in three Japanese Black populations (n=560, 245 and 287) and a Holstein population (n=202). Tukey-Kramer's honestly significant difference test revealed that CC genotype in c.267C>T indicated lower C14:0 and higher C18:1 than the other genotypes in Japanese Black cattle and CC genotype in c.866C>T showed lower C16:1 than CT genotype in Holstein cattle (P<0.05). These results suggested that these genotypes would contribute to production of high-grade meat as selection markers in beef cattle.
WILEY-BLACKWELL, 2014年05月, ANIMAL SCIENCE JOURNAL, 85 (5), 499 - 505, 英語[査読有り]
研究論文（学術雑誌）
Genetic factors are known to influence meat quality in beef cattle. Expression of fatty acid desaturase 2 (FADS2) and fatty acid-binding protein 4 (FABP4) genes in the fat metabolism pathway has been reported to influence fat-related traits. In this study, we aimed to discover DNA polymorphisms in the promoter regions of the FADS2 and FABP4 genes in cattle populations to develop genetic markers. As a consequence, one single nucleotide polymorphism (SNP) in the FADS2 promoter (FADS2 g.-823G > A) and two linked SNPs in the FABP4 promoter (FABP4 g.-295A > G and FABP4 g.-287A > G) were identified. All of them were assumed to influence binding sites for transcription factors, suggesting these SNPs influence the genes' expression and beef quality. Therefore, we performed association analysis between the genotypes and traits. Our results revealed that the FADS2 g.-823G > A had significant effects on several traits including beef marbling score (BMS). BMS of the G/G animals in a Holstein population (2.36) was higher than that of the G/A animals (2.13). The same tendency was observed in Japanese Black populations. On the other hand, although the FABP4 SNPs also had significant effects on some traits, they did not show additive effects on any of the traits analyzed in this study. Our results suggest that the FADS2 g.-823G > A is a useful genetic marker for breeding in beef cattle. (C) 2014 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2014年05月, LIVESTOCK SCIENCE, 163, 34 - 40, 英語[査読有り]
研究論文（学術雑誌）
- 兵庫県黒毛和種肥育集団における経済形質関連遺伝子の効果
本研究では、兵庫県黒毛和種肥育集団の個体539頭を用い、SCD、SREBP-1、FASN、EDG-1、F11、MC1R、CW-2の遺伝子頻度を求め、脂肪酸組成および枝肉格付形質に対する遺伝子効果を調査した。優良アリル頻度はSCDで0.960、SREBP-1で0.322、FASNで0.910、EDG-1で0.265、F11で0.623、MC1Rで0.464、CW-2で0.007であった。SCD、SREBP-1、FASN、EDG-1では脂肪酸組成に、MC1Rでは枝肉重量に対し効果が認められた。SCDではAアリル、SREBP-1ではLアリルに不飽和脂肪酸を増加する効果が認められた。MC1RではED/ED遺伝子型において枝肉重量が大きい効果が認められた。経済形質関連遺伝子の遺伝子頻度や効果の大きさは集団ごとに異なるため、全国の黒毛和種の改良に大きく寄与した兵
日本畜産学会, 2013年11月, 日本畜産学会報, 84 (2), 157 - 162, 日本語[査読有り]
研究論文（学術雑誌）
- (公社)日本畜産学会, 2013年09月, 日本畜産学会大会講演要旨集, 117回, 65 - 65, 日本語兵庫系黒毛和種における育種集団毎の遺伝的多様性評価
In our previous study, we examined divergently selected regions between Japanese Black cattle and Holstein cattle based on a 50k single nucleotide polymorphism (SNP) panel and a Sliding Window Allele Difference method resulting in the identification of 11 genomic regions. The aim of the present study was to investigate the association between these genomic regions and economic traits, including seven carcass and five milk production traits. For this purpose, representative SNP markers were selected from the 11 genomic regions and used to estimate the effects on the traits in Japanese Black cattle (N=488) and Holstein cattle (N=194). Association analyses revealed that five SNPs showed a significant effect on the carcass traits in Japanese Black cattle and other five SNP showed a significant association with milk production traits in Holstein cattle (P< 0.05). These results indicated that divergently selected regions identified using a Sliding Window Allele Difference method contain the quantitative trait loci (QTL) for economic traits. Furthermore, most of the QTL identified in this study were consistent with the previously reported QTL. Further investigations of these regions may lead to the identification of the genes and polymorphisms responsible for the economic traits. © 2013 Elsevier B.V.
Elsevier, 2013年08月, Livestock Science, 155 (2-3), 180 - 185, 英語[査読有り]
研究論文（学術雑誌）
- 黒毛和種の背最長筋を用いた網羅的遺伝子発現解析
黒毛和種の遺伝的改良において、近年脂肪酸組成が食味性への影響から脚光を浴びている。本研究は脂肪酸組成に差のある集団間で発現量の異なる遺伝子を同定し、育種選抜を推進するために必要なDNA マーカーの候補とすることを目的とした。この目的のため、日本各地から集められた黒毛和種集団を筋肉内一価不飽和脂肪酸 (MUFA)含有率が高いグループと低いグループに分け、マイクロアレイによる網羅的遺伝子発現解析を行った。その結果、SPP1 (secreted phosphoprotein-1)、LEP (leptin)、MMP14 (matrix metallopeptidase 14)、IGF1 (insulin-like growth factor-1)、FGF1 (fibroblast growth factor-1)、FGF2 など脂肪酸代謝に関与すると考えられ
日本動物遺伝育種学会, 2013年06月, 動物遺伝育種研究, 41 (1), 7 - 14, 日本語[査読有り]
研究論文（学術雑誌）
Genetic analyses have contributed to improvements of economically important traits derived from adipose tissue such as fatty acid composition in beef. Elongation of very long chain fatty acids (ELOVL) genes encode for the enzymes that play an important role in elongation of long-chain fatty acids. In this study, we aimed to discover genetic polymorphisms of ELOVL gene family in cattle populations to develop genetic markers. As a result, five synonymous mutations were detected in the coding regions of the ELOVL1, ELOVL2, ELOVL3 and ELOVL5 genes. In addition, six mutations were identified in promoter region of the ELOVL5. Two of five mutations in the promoter region of ELOVL5 were expected to alter the ELOVL5 expression and influence the economic traits, because of the high synteny of the region which was essential for activation of Elovl5 in mouse. Therefore, we performed association analysis between the genotypes and traits and our result revealed that T allele of g.-110T > C in ELOVL5 gene promoter indicated significantly thinner subcutaneous fat thickness (TT, 2.39 cm; CT, 2.35 cm) than that of C allele (CC, 2.68 cm) in a Japanese Black population. Our results suggest that the g.-110T > C is a useful genetic marker for the breeding in beef cattle.
SPRINGER, 2013年04月, MOLECULAR BIOLOGY REPORTS, 40 (4), 3231 - 3237, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition is an important economic trait for both dairy and beef cattle and controlled by genetic factors. Candidate genes controlling fatty acid composition may be found in fat synthesis and metabolism pathways. Acetyl-CoA carboxylase is the flux-determining enzyme in the regulation of fatty acid synthesis in animal tissues. One of two isozymes of this enzyme, acetyl-CoA carboxylase-alpha (ACACA), catalyses the first committed step of fatty acid synthesis in mammalian cytosol, leading to the biosynthesis of long-chain fatty acids. In the current study, the sequence comparison of the coding sequence (CDS) and two promoter regions (PIA and PIII) in bovine ACACA gene was performed between Japanese Black and Holstein cattle to detect nucleotide polymorphisms influencing fatty acid composition in milk and beef. Five single nucleotide polymorphisms (SNPs) were identified in the CDS region, 28 SNPs in the PIA region and three SNPs in the PIII region. Association study revealed that CCT/CCT type of PIII_#1, #2/PIA_#26 indicated a higher percentage of C14:0 in the milk of the Holstein cattle than CCT/GTC type (p = 0.050) and that a difference of the percentage of C16:0 was observed between CCT/CCT and GTC/GTC type (p = 0.023). CDS_#2 T/T type indicated a higher percentage of C18:0 than T/C type (p = 0.008). In addition, the Japanese Black cattle with CC/GT type of PIII_#1, #2 showed a higher percentage of C18:2 in the meat than those with GT/GT type (p = 0.025). Since PIII is the promoter specific to mammary gland during lactation, the altered expression of the ACACA gene owing to the SNPs in the PIII region may influence the fatty acid composition in the milk.
SPRINGER, 2012年09月, MOLECULAR BIOLOGY REPORTS, 39 (9), 8637 - 8644, 英語[査読有り]
研究論文（学術雑誌）
The estimated allele frequency at neutral DNA marker loci is a fundamental parameter for establishing a conservation scheme for a set of livestock breeds. In this study, we propose a novel minimum distance (MD) method for estimating neutral allele frequencies, which minimizes the error by the use of pedigree information. Using computer simulation and actual microsatellite data for a pedigreed cattle population, the performance of the proposed method was compared with that of conventional random sampling (RND). MD manifests a promising superiority to RND, irrespective of the sample size and the depth of pedigree. It is theoretically demonstrated that the error of the estimates depends on both the sample size and the distance of founder allele frequencies between the sampled and the non-sampled individuals. MD samples individuals so as to minimize the latter source of estimation error.
WILEY-BLACKWELL, 2012年06月, JOURNAL OF ANIMAL BREEDING AND GENETICS, 129 (3), 226 - 233, 英語[査読有り]
研究論文（学術雑誌）
FASN is a multifunctional enzyme that catalyzes de novo biosynthesis of long-chain saturated fatty acid, and alteration of this enzyme may affect economic traits including fatty acid composition. Sequencing the coding sequence (CDS) of the FASN gene has so far been done only partially for dairy cattle. In this study, the CDS of the FASN gene of eight Holstein cattle was sequenced to detect nucleotide polymorphisms. We could identify 13 single nucleotide polymorphisms (SNPs) of which five were non-synonymous mutations (H1390Y, I1856L, T1950A, W1955R and T2264A). The association between these SNPs and economic traits was analyzed using 198 Holstein dams. Genotypes of H1390Y corresponded to those of the I1856L in the group, suggesting these SNPs were linked. Furthermore, the T1950A was suggestively linked with the W1955R. The association study revealed that T1950A/W1955R had an effect on milk fat content and the C14 Index. This will help to understand the complex association between the FASN gene and economic traits. (C) 2011 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2012年04月, LIVESTOCK SCIENCE, 144 (3), 281 - 284, 英語[査読有り]
研究論文（学術雑誌）
Differences between average allelic frequencies of genes that relate to traits suggest that it would be evidence of artificial selections. Sliding window approach is a useful method to identify genomic regions that have been differently selected between two breeds. The objective of this study was to identify the divergently selected regions between Japanese Black (JB) and Japanese Holstein (JH) cattle based on genotypic information obtained through a high-density single nucleotide polymorphism (SNP) panel. After genotyping of 54 001 SNP markers on 100 animals (50 JB and 50 JH), 40 635 SNPs were suitable for the analysis. For each of these SNPs, the absolute difference between allelic frequencies of JB and JH was calculated. In the current study, 10 consecutive SNPs were defined as components of a window. For each window, the average difference in allelic frequency was calculated. This was termed sliding window average difference (SWAD). Among 40 055 windows, we focused on 39 windows with the largest SWAD. This was equivalent to 0.1% of all windows and the SWAD was more than 0.435. Some of these windows overlapped and were distributed in 11 regions. These regions were in good agreement with reported quantitative trait locus, therefore would be selection signatures and good candidates that harbor the causative mutations.
WILEY-BLACKWELL, 2012年, ANIMAL SCIENCE JOURNAL, 83 (1), 7 - 13, 英語[査読有り]
研究論文（学術雑誌）
- 日本畜産学会, 2011年11月, 日本畜産学会報, 82 (2), 125 - 130, 日本語黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果
[査読有り]
研究論文（学術雑誌）
Fatty acid composition and carcass traits of 2,275 Japanese Black steers and heifers were analyzed to estimate the heritabilities and genetic correlations using the REML procedure. Slices of LM at the 6th to 7th rib section were minced and homogenized, and total lipids were extracted for the analysis by a gas chromatograph. Oleic acid accounted for the majority (51.3%), followed by palmitic (26.4%) and stearic (10.8%) acids. Heritabilities of carcass traits were moderate to high, ranging from 0.34 to 0.61, and heritabilities of individual fatty acids varied largely from 0.00 to 0.78. Those of MUFA, SFA, and PUFA were estimated to be 0.68, 0.66, and 0.47, respectively. Predicted breeding values for MUFA in 99 sires ranged from -3.0 to 5.4%. Genetic correlations of fatty acid compositions with carcass traits were generally weak (-0.28 to 0.39). Low but positive genetic correlations were obtained between beef marbling, on which emphasis of selection has been placed, and oleic acid (0.19) or MUFA (0.23). The results indicated the possibility not only for genetic improvement in fat quality traits but also simultaneous improvements with carcass traits by appropriate selection program.
AMER SOC ANIMAL SCIENCE, 2011年03月, JOURNAL OF ANIMAL SCIENCE, 89 (3), 615 - 621, 英語[査読有り]
研究論文（学術雑誌）
- 2011年, 肉用牛研究会報, 90, 10 - 14, 日本語黒毛和種における分娩後90日までの泌乳量の推定
[査読有り]
研究論文（学術雑誌）
In the meat industry, correct labeling of beef origins or breed is required to assure quality and safety. This paper describes the development of discrimination markers between Japanese domestic and imported beef from the United States (US) and Australia (AUS) based on a bovine 50K single nucleotide polymorphism (SNP) array using a total of 110 samples: Japanese Black (n = 50), Japanese Holstein (n = 50) and US cattle (n = 10). Genotyping information revealed 1081 SNPs as candidate markers that were polymorphic only in US cattle. The genotyping results by PCR - restriction length polymorphism in Japanese Black (n = 300) and Holstein cattle (n = 146) revealed that 11 SNPs had alleles specific to US cattle. Their allelic frequencies in US cattle (n = 108) ranged from 0.097 to 0.250 with an average of 0.178 and the combined identification probability of US cattle was 0.987. In addition, we also verified the applicability of these US-specific markers to AUS cattle. Their allelic frequencies in AUS cattle (n = 280) ranged from 0.063 to 0.224 with an average of 0.137 and the combined identification probability of AUS cattle was 0.963. In conclusion, a set of these markers could be useful for discriminating between Japanese domestic and imported beef and would contribute to identify origins and prevent falsified labeling of beef.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (1), 67 - 72, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition of adipose tissue has been recognized as an important carcass trait because of its relationship with eating quality such as favorable beef flavor and tenderness. Therefore, we investigated the effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) on fatty acid composition in intramuscular fat tissue of Holstein steers. The major allele frequencies were 0.705 in SCD, 0.518 in FABP4, 0.888 in FASN, and 0.984 in LXR. Genotyping of SCD showed significant effect on C14:0, C14:1, C18:0 and saturated fatty acid (P < 0.05). In addition, the result suggested that SCD genotype possibly had effect on composition of C18:1 and monounsaturated fatty acid. Genotype of FABP4 had significant effect on composition of C16:0. Effect of LXR genotypes could not be analyze because of extremely biased genotype frequencies. Our results suggest that genotypes of SCD and FABP4 may in part affect meat quality in Holstein.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (3), 406 - 411, 英語[査読有り]
研究論文（学術雑誌）
Allele frequencies of 10 representative polymorphisms for beef and milk traits were investigated for a total of 240 animals from Bos taurus and Bos indicus breeds, including two Japanese groups (Japanese Black and Japanese Brown), two East Asian groups (Korean and Mongolian), three European groups (Holstein, Angus and Hereford) and a Bos indicus group in South Asia (Myanmar, Laos and Cambodia). The Japanese Black revealed unique genetic construction in GH, FASN and SREBP-1 and the other Asian populations show intermediate frequencies between European and Japanese populations. The Bos indicus group showed low favorable allele frequencies in most of the genes. The study showed the variability and distribution of 10 genes affecting economic traits among world representative cattle breeds. The genetic information would contribute to elucidating the genetic background for worldwide cattle breeds and the possibility of improvement using the markers.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (6), 717 - 721, 英語[査読有り]
研究論文（学術雑誌）
- Suppression of WWP1 Gene Via RNAi Induced the Reduction of Proliferation Rate of C2C12 Myoblasts
The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), which plays an important role in ubiquitin-proteasome pathway to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Previously, we transfected either of wild and mutated types of WWP1 gene into C2C12 mouse myoblasts to monitor the expression pattern of muscle-differentiation markers, so that excessive WWP1 expression enhanced the expression of the myosin heavy chain (MyHC) Ia gene but lowered the expression of the MyHC IIb gene, while mutated WWP1 gene transfected in to myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. However, the mechanism for the mutation to inhibit muscle differentiation remains elucidated. The current study attempted to suppress the WWP1 expression by RNAi technique and to observe its effect on C2C12 cells. The effect of WWP1 suppression was clearly different from that of the R441Q missense mutation in the WWP1 gene. The WWP1 suppression reduced the proliferation rate of C2C12 myoblasts, while clear difference was not observed in the proliferation rate by the transfection of the mutated WWP1 gene into the cells. Our RT-PCR analysis indicated that the reduction of the WWP1 expression is the specific effect mediated by RNAi and that the reduction of proliferation rate observed in this study is largely attributed to the suppression of the WWP1 expression. These data indicated that the mutation responsible for chicken muscular dystrophy does not eliminate the enzymatic activity and provides some new function for the gene.
JAPAN POULTRY SCIENCE ASSOC, 2010年10月, JOURNAL OF POULTRY SCIENCE, 47 (4), 288 - 293, 英語[査読有り]
研究論文（学術雑誌）
- Japanese Society of Animal Genetics, 2010年06月, Journal of Animal Genetics, 38 (1), 77 - 81, 英語Mitochondrial DNA variation and genetic construction of indigenous cattle population in Bhutan
[査読有り]
研究論文（学術雑誌）
- 2010年, The Journal of Animal Genetics, 38 (2), 73 - 76, 英語Genetic variability of semen characteristics in Japanese Black bulls
[査読有り]
研究論文（学術雑誌）
Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.
WILEY-BLACKWELL PUBLISHING, INC, 2010年, ANIMAL SCIENCE JOURNAL, 81 (2), 145 - 151, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle.
WILEY-BLACKWELL PUBLISHING, INC, 2009年06月, ANIMAL SCIENCE JOURNAL, 80 (3), 225 - 232, 英語[査読有り]
研究論文（学術雑誌）
- Number of Calves Produced at Specified Age as a Measure of Reproductive Performance in Beef Cattle under Artificially-Inseminated Breeding Scheme
Reproductive abilities in beef cattle herds are receiving increased attention due to recent rises in production costs. To achieve more efficient management, a measure of fertility, namely the number of calves produced at k yr of age (NCPk), was developed and its genetic parameters were estimated from Japanese Black cows by restricted maximum likelihood procedures. The k examined were distributed from 2 to 10 yr of age and NCP(2) averaged 1.077 calves over 43,536 cows. The averages increased by approximately 0.9 calf with each additional 1 yr increment in k. Heritabilities of NCP(k) were estimated to be low ranging from 0.083 to 0.162, which seemingly suggested a difficulty of genetic improvement. However, large genetic variation and high accuracy were observed in predicted breeding values of NCP(k). For example, the breeding values of NCP(7) were predicted between -0.303 and +0.213 with average accuracy of 0.607 for cows with observations. Genetic correlations among different k were generally high and positive (0.474 to 0.995). The analyses showed that at least NCP(4) was required to maintain the genetic correlations of 0.8 or higher with subsequent NCP(k). Also NCP(5) maintained the genetic correlations of 0.9 or higher with subsequent NCP(k). The results suggested some possibilities for NCP(k) to be a selection criterion considering its genetic variation, high accuracy and consistency with subsequent performance.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2009年02月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 22 (2), 162 - 167, 英語[査読有り]
研究論文（学術雑誌）
Background: Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding protein (FABP4), liver X receptor alpha (LXR alpha), cytochrome b(5) (Cyt b(5)), long-chain acyl-CoA synthetase (ACSL) 1, ACSL4 and diacylglycerol acyltransferase 2 (DGAT2) and fatty acid composition. Results: Sequence comparisons revealed 14 single nucleotide polymorphisms in six genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXR alpha, were nonsynonymous substitutions. The associations between the genotypes and fatty acid compositions were analyzed by using 234 Japanese Black cattle. The genotypes of FABP4 I74V and LXR alpha V133I were significantly associated with palmitoleic acids (C16: 1, P = 0.0086) and linoleic acid (C18:2, P = 0.0121) content in intramuscular fat, respectively. Conclusion: Our findings suggest that the two polymorphisms of FABP4 I74V and LXR alpha V133I might be genetic factors in part associated with palmitoleic acid (FABP4 I74V) and linoleic acid (LXR alpha V133I) composition in intramuscular fat of Japanese Black cattle, respectively. Especially, FABP4 I74V had highly significant effect (P < 0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.
BIOMED CENTRAL LTD, 2008年12月, BMC GENETICS, 9, 84, 英語[査読有り]
研究論文（学術雑誌）
The performance test protocol for Japanese Black cattle was revised in April 2002. This resulted in restriction of access to concentrate (based on body weight) and modification of the concentrate's ingredients. Genetic parameters of growth and feed utilization traits of the performance test were estimated using 1304 records using the revised protocol. Residual feed intakes (RFIs) as alternative indicators for feed utilization efficiency were included. (Co)variance components were estimated by EM-REML. Heritabilities for growth traits were between 0.26 and 0.47. Heritabilities for feed intakes and conversions ranged from 0.25 to 0.37 and from 0.03 to 0.29, respectively. Genetic variances and heritabilities were lower for the revised protocol. Highly positive genetic correlations of daily gain (DG) with feed intakes indicated selection on DG is expected to increase feed intake. Selection on feed conversion may lead to higher DG. The heritability estimates for RFIs ranged from 0.10 to 0.33 and were generally higher than corresponding estimates for feed conversion ratios. RFI of TDN showed positive genetic correlations with all feed intakes. The reduction of feed intakes could be expected through selection on the RFI without changing body size. RFIs were considered to be alternative indicators to improve feed utilization efficiency under the new performance test.
WILEY-BLACKWELL, 2008年06月, ANIMAL SCIENCE JOURNAL, 79 (3), 291 - 296, 英語[査読有り]
研究論文（学術雑誌）
To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.
SPRINGER, 2007年12月, MAMMALIAN GENOME, 18 (12), 880 - 886, 英語[査読有り]
研究論文（学術雑誌）
Alternative breeding strategies were simulated based on the population structure of the Tajima strain of Japanese Black cattle. An analysis of the population structure revealed that some sires up to 20 years of age have been used in Tajima. In addition, 95% of newborn calves were the progeny of only 20 sires, and their mating frequencies were significantly skewed. The current average inbreeding coefficient and founder genome equivalents of the strain were estimated to be 0.199 and 2.25, respectively. Average inbreeding coefficient is expected to reach 0.394 within 27 years. Thus, different breeding strategies were assessed for their effect on the level of inbreeding and average genetic merit. We compared strategies that (1) halve the sire service period, (2) double the number of mating sires and (3) lower the skewed sire mating frequency and optimize the frequency for weighted genetic merit and diversity. Reducing the service period yielded a 7.0-12.0% reduction in the rate of inbreeding while maintaining almost the same genetic gain. Increasing the number of sires resulted in a 19.3-21.3% reduction in inbreeding with a corresponding 1.6-8.4% reduction in gain. The rates of inbreeding from the optimized strategies decreased as the weight on genetic diversity increased. However, a strategy that emphasized only genetic gain yielded lower gain than other strategies because the strategy allowed only one sire to mate, resulting in reduced genetic variance and low accuracy of genetic evaluation. In contrast, a strategy with no emphasis on genetic gain when determining mating frequency resulted in reductions of 16.0% and 63.2% in genetic gain and inbreeding, respectively. The strategies examined here are easily applicable and can be expected to reduce immediate loss of genetic diversity.
BLACKWELL PUBLISHING, 2007年04月, JOURNAL OF ANIMAL BREEDING AND GENETICS, 124 (2), 73 - 80, 英語[査読有り]
研究論文（学術雑誌）
Heritabilities of and genetic correlations between additive direct and maternal genetic effects for calf market weight, and additive direct genetic effects for carcass traits, were estimated for Japanese Black cattle by REML procedures under 2-trait animal models. Data were collected from calf and carcass markets in Hyogo and Tottori prefectures and analyzed separately by prefecture. Calf market weight was measured on 42,745 and 23,566 calves in Hyogo and Tottori, respectively. Only the fattening animals with calf market weight were extracted from the carcass database and used for estimation. The carcass traits analyzed were carcass weight, ribeye area, rib thickness, subcutaneous fat thickness, yield estimate, beef marbling score, and 4 meat characters (color, brightness, firmness, and texture). Direct and maternal heritabilities for calf market weight were estimated to be 0.22 and 0.07 in Hyogo, and 0.37 and 0.15 in Tottori, respectively. The estimates of heritabilities for carcass traits were moderate to high in both prefectures. The estimates of direct-maternal genetic correlations for calf market weight were positive (0.17) in Hyogo and negative (-0.63) in Tottori. The direct effect for calf market weight was positively correlated with the direct effect for carcass weight (0.87 and 0.56 in Hyogo and Tottori, respectively) but negatively correlated with the direct effect for beef marbling score (-0.10 in both prefectures). The estimates of genetic correlations between the maternal effect for calf market weight and the direct effects for carcass traits varied from -0.13 to 0.34 in Hyogo and from -0.14 to 0.15 in Tottori. Because direct and maternal genetic effects for early growth traits can be evaluated from calf market weight data in the production system of Japanese Black cattle, this information should be incorporated into selection and mating schemes of the breed.
AMER SOC ANIMAL SCIENCE, 2006年10月, JOURNAL OF ANIMAL SCIENCE, 84 (10), 2617 - 2622, 英語[査読有り]
研究論文（学術雑誌）
We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.
SPRINGER, 2005年03月, MAMMALIAN GENOME, 16 (3), 201 - 210, 英語[査読有り]
研究論文（学術雑誌）
We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.
SPRINGER, 2005年03月, MAMMALIAN GENOME, 16 (3), 201 - 210, 英語研究論文（学術雑誌）
- 但馬牛集団のジーンドロッピング法による系統分類の試み
但馬牛における始祖個体遺伝子の消長をジーンドロッピング法で調べ，その結果を基に普遍的な系統分類の方法を検討した．1 現存牛の血統を可能な限り遡り出現した牛のうち名号，生年月日が明らかで，産地が兵庫県内の牛を始祖個体としたところ，条件を全て満たす個体は1,461頭(累計遺伝的寄与率0.773)となった．系統分類のための始祖個体は，Pr(lost)の小さい100頭に限定した．2 始祖個体の生産地は城崎郡14頭(累積寄与率0.0314)，出石郡2頭(累積寄与率0.0002)，美方郡82頭(累積寄与率0.637)と養父郡2頭であったが，当時の郡境からは養父郡の2頭は美方郡に分類されていると考えられ，美方郡産が84頭にのぼり寄与率が高かった．3 現存個体27,138頭に対する始祖個体からの遺伝的寄与率を計算し，その結果を基に主成分分析を行った．第3主成分までで約85％を説明でき，現在個体の頭数割合が3.7～25.9％の8グループに分類できた．4 各グループ内及びグループ間の平均血縁係数から，G1～G4とG8ではグループ間の平均血縁係数がグループ内の平均血縁係数よりも高いグループが存在し，近年は系統外の種雄牛との交配が増加したことに起因すると考えられた．G5～G7では，グループ内の平均血縁係数が最も高く，依然，系統内交配が主流であると考えられた．5 グループ毎の近交係数は，16,143頭の結果からG1～G4では13.0～17.8％と低く，G5～G8で18.7～23.2％と高い傾向にあった．6 グループ毎の枝肉重量の育種価の平均は-8.1～-2.7とグループ間でばらついていた．BMS育種価の平均に関しても，0.63～1.00とばらついていたが，各グループともに最大値をみると1.477～2.137と能力が高い個体から含まれていた．以上の結果から，ジーンドロッピング法により選定した100頭の始祖個体を元にした系統分類法によって現在の但馬牛を分類することが可能であり，頭数や産肉能力から見ても遺伝的多様性を保持しつつ，今後の改良が可能であることが明らかとなった．
兵庫県立農林水産技術総合センター, 2005年, 兵庫県立農林水産技術総合センター研究報告〔畜産編〕, 41 16-21 (41), 16 - 21, 日本語[査読有り]
研究論文（学術雑誌）
Production traits of a Rhode Island Red layer line were analyzed to detect carrier animals with major genes that influenced a particular trait by applying the major gene index (MGI) approach. This method was used to examine the deviation of predicted breeding values between parents and offspring. Advantages of this method include its simplicity and time-reducing, cost-saving benefits compared with any other statistical approach for screening major genes. The layer line had been selected based on the desired gain index for 6 years at the National Livestock Breeding Center, Okazaki station. The line consisted of 125 sires and 2986 dams. Two economic traits as breeding objectives were studied - age at sexual maturity (ASM, days) and egg production efficiency (EP, %). The MGI detected nine sires and 23 dams as carriers with major genes for ASM, and five sires and 26 dams for EP. They were identified as important breeding stock, and pedigree information provided candidates possessing major genes with favorable effect at the founding of the selected layer line. It seems likely that the simple approach of the MGI could be a useful preliminary method for detecting carrier animals with major genes before applying molecular techniques on a sampled population to identify in more detail the existence of major genes and their carriers.
Blackwell Publishing, 2005年, Animal Science Journal, 76 (3), 195 - 201, 英語[査読有り]
研究論文（学術雑誌）
Japanese Black cattle are characterised by a unique ability to deposit intramuscular fat with lower melting temperature. In this study, 3 consecutive biopsies from Longissimus muscle tissue were taken and RNA isolated from 3 Japanese Black (Tajima strain) and 3 Holstein animals at age 11 - 20 months. The gene expression changes in these samples were analysed using a bovine fat/muscle cDNA microarray. A mixed-ANOVA model was fitted to the intensity signals. A total of 335 (4.8%) array elements were identified as differentially expressed genes in this breed x time comparison study. Genes preferentially expressed in Japanese Black are associated with mono-unsaturated fatty acid synthesis, fat deposition, adipogenesis development and muscle regulation, while examples of genes preferentially expressed in Holstein come from functional classes involved in connective tissue and skeletal muscle development. The gene expression differences detected between the Longissimus muscle of the 2 breeds give important clues to the molecular basis for the unique features of the Japanese Black breed, such as the onset and rate of adipose tissue development, metabolic differences, and signalling pathways involved in converting carbohydrate to lipid during lipogenesis. These findings will impact on industry management strategies designed to manipulate intramuscular adipose development at different development stages to gain maximum return for beef products.
C S I R O PUBLISHING, 2005年, AUSTRALIAN JOURNAL OF EXPERIMENTAL AGRICULTURE, 45 (7-8), 809 - 820, 英語研究論文（学術雑誌）
Pedigree analysis of the 77 advanced Indian potato selections showed that their origin could be traced to only 49 ancestors out of which 29 were exotic, which accounted for 69.52% of the total genomic constitution and maximum contribution (40.65%) was by 10 ancestors from U.K. Breeders' tendency was to involve adapted advanced clones as immediate parents. Coefficient of relationship between pair of selections ranged from 0 to 0.68. The success of the parents used was not related to region-specific choice of parents. The findings are discussed in the context of "genetic uniformity" and the relevance of having separate breeding programmes for the three major areas of potato cultivation in India. Attempt has also been made to give some directions for choosing selections for use as parents and release as cultivars based on their coefficient of relationship so as to broaden the genetic base of the future potato cultivars.
SPRINGER, 2005年01月, EUPHYTICA, 142 (1-2), 23 - 31, 英語[査読有り]
研究論文（学術雑誌）
- Genealogical relationship between pedigree and microsatellite information and analysis of genetic structure of a highly inbred Japanese black cattle strain
Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated-low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, 2004年10月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17 (10), 1355 - 1359, 英語研究論文（学術雑誌）
- Trace of native cattle in Japanese Holstein assessed by mitochondrial DNA sequence polymorphism
On the basis of sequence variation in the displacement loop region of mtDNA, 588 Japanese and North American Holstein cows were classified into 5 mitochondrial haplotypes, which were found in Japanese Black cattle. One of the haplotypes (named type 1), which was present at the highest frequency in Japanese Black cattle, was not observed in either European or African cattle. This haplotype is characterized by 2 single-nucleotide polymorphisms. One is called the type B polymorphism, and it refers to a base change from T to C at nucleotide 16042 of the mitochondrial genome (T160042C). The other is called the type I polymorphism, and it refers to the base change as G16093A. The proportion of the Japanese Holstein population with both polymorphisms was 18.3%, whereas none of the North American cows had this genotype. Because the mitochondrial types were inherited maternally, it is clear that a considerable number of Japanese Holstein cows are descended from native Japanese cattle. Polymorphisms B and I accounted for no variance in the estimated breeding value for milk production among cows from the Hyogo herd (582 cows) or the Chiba region herd (758 cows). This result suggests that most autosomal genes of native animals have been successively replaced by those of pure Holstein after grading up of over 15 generations, even though resulting animals have native animal-oriented mitochondrial types and may still have some number of the native autosomal genes.
AMER DAIRY SCIENCE ASSOC, 2004年09月, JOURNAL OF DAIRY SCIENCE, 87 (9), 3071 - 3075, 英語研究論文（学術雑誌）
The effect of cattle breed on stearoyl-CoA desaturase (SCD) gene expression was investigated in this study. Detailed comparisons of SCD mRNA level were made among three steers each of Japanese Black, Holstein and their crossbreed which were age-matched had been fed the same diet and were sampled by biopsy of the longissimus dorsi (LD) muscle and subcutaneous fat. The levels of SCD mRNA were measured in samples of muscle and subcutaneous fat. The levels of SCD mRNA demonstrated a breed effect in each tissue, though the relative expression was higher (P < 0.05) in subcutaneous fat. The ratio of SCD mRNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA for Japanese Black, crossbreed and Holstein were 132.1 +/- 34.1, 73.5 +/- 22.7 and 39.5 +/- 12.9, respectively, and significant (P<0.05) differences existed between Japanese Black and Holstein cattle. Japanese Black subcutaneous fat had consistently higher (P<0.05) monounsaturated fatty acids (MUFA) percentage than Holstein subcutaneous fat. These results suggest that differences in SCD gene expression may contribute to the fatty acid compositional differences seen between subcutaneous adipose tissue of Japanese Black cattle and Holstein. (C) 2003 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2004年05月, LIVESTOCK PRODUCTION SCIENCE, 87 (2-3), 215 - 220, 英語研究論文（学術雑誌）
To investigate the genetic factors that affect fatty acid composition of beef, we compared the full-length bovine stearoyl-CoA desaturase (SCD) cDNA from 20 Japanese Black steers. Two types of the SCD gene with single nucleotide polymorphisms (SNPs) were observed in the ORF of SCD cDNA, in which an amino acid replacement from valine (type V) to alanine (type A) was predicted. We developed a method for genotyping these two SCD genes based on PCR-RFLP. We have classified 1003 Japanese Black carcasses into three genotypes, VV, VA, and AA, and compared fatty acid composition among them. The SCD type A gene contributed to higher MUFA percentage and lower melting point in intramuscular fat. The SCD genotype was not the only genetic factor contributing to fatty acid composition of Japanese Black steers, but the SCD genotype was considered one of the causes of genetic variation in fatty acid composition of Japanese Black steers. Transcription factors such as sterol regulatory element binding protein-1c (SREBP-1c) may account for the remaining part of the genetic variation in fatty acid composition.
SPRINGER-VERLAG, 2004年02月, MAMMALIAN GENOME, 15 (2), 142 - 148, 英語研究論文（学術雑誌）
Using amplified fragment length polymorphism (AFLP) fingerprinting, selective genotyping was performed to determine if this method was effective for selecting superior breeding stock. Forty-eight cows with extreme genetic merit for beef marbling score (BMS) were selected from a population of Japanese Black cattle (n = 4462), including 25 with the highest for predicted breeding value (PBV) and 23 with the lowest. Sixteen AFLP fragments were selected for further analysis based on fragment frequency differences between the high and low groups. A linear discriminant analysis using these AFLP fragments was applied in order to derive a discriminant function that classified the cows into high and low groups. Seven of the 16 fragments were included in the resulting function and the discriminant scores (general genetic values, GGV) of the 48 cows were calculated using the function. These cows were clearly separated into high and low groups by GGV with a correlation ratio of 0.91 (discriminative error of 2.1%). The same function was then applied to 121 additional cows that were randomly selected from the original population. A significant regression coefficient of GGV on BMS-PBV (R-2 = 0.45) was obtained, which indicates that the GGV can be used as a selection criterion for BMS in this population. These results suggest that AFLP fingerprinting can be used for animal breeding without identifying the underlying genes affecting the trait of interest.
BLACKWELL PUBLISHING LTD, 2004年02月, ANIMAL GENETICS, 35 (1), 40 - 43, 英語研究論文（学術雑誌）
On the basis of sequence variation in the displacement loop region of mtDNA, 588 Japanese and North American Holstein cows were classified into 5 mitochondrial haplotypes, which were found in Japanese Black cattle. One of the haplotypes (named type 1), which was present at the highest frequency in Japanese Black cattle, was not observed in either European or African cattle. This haplotype is characterized by 2 single-nucleotide polymorphisms. One is called the type B polymorphism, and it refers to a base change from T to C at nucleotide 16042 of the mitochondrial genome (T160042C). The other is called the type I polymorphism, and it refers to the base change as G16093A. The proportion of the Japanese Holstein population with both polymorphisms was 18.3%, whereas none of the North American cows had this genotype. Because the mitochondrial types were inherited maternally, it is clear that a considerable number of Japanese Holstein cows are descended from native Japanese cattle. Polymorphisms B and I accounted for no variance in the estimated breeding value for milk production among cows from the Hyogo herd (582 cows) or the Chiba region herd (758 cows). This result suggests that most autosomal genes of native animals have been successively replaced by those of pure Holstein after grading up of over 15 generations, even though resulting animals have native animal-oriented mitochondrial types and may still have some number of the native autosomal genes.
American Dairy Science Association, 2004年, Journal of Dairy Science, 87 (9), 3071 - 3075, 英語研究論文（学術雑誌）
- 2004年, Proc. 29th Int. Conf. on Anim. Genet., p.138, 英語Selective genotyping for marker assisted selection in Japanese Black cattle.
研究論文（国際会議プロシーディングス）
- 2004年, Proc. 29th Int. Conf. on Anim. Genet., p.70, 英語Comparisons between Japanese Black and Holstein cattle using gene expression profiling.
研究論文（国際会議プロシーディングス）
- 但馬牛集団の血縁係数による系統分類の試み
血縁係数を利用したクラスター分析により但馬牛集団の系統分類を試み、現存集団の遺伝的構成を把握し、系統の再構築の可能性を検討した。とくに、今回の分析で規模が小さな集団の増頭を目的として、仮想集団を作出した場合の系統分類についても検討した。1.供用中集団のデンドログラムを血縁係数0.10で切断し、7グループに分類した。2.各グループ内及びグループ間の平均血縁係数は、グループ1、2、3と5では全て0.3以上であった。グループ4も概ね0.3で上記グループとの血縁が高かった。グループ6と7が、他グループと異なりグループ間の血縁係数が低かった。3.各グループの育種価評価値の平均値を比較するとグループ毎に産肉あるいは種牛能力の特徴が明らかになった。4.600頭の仮想雌牛群と待機雄牛の後代を加え系統分類を実施した。その結果、中土井系繁殖雌牛に城崎系種雄牛を交配するシミュレーション集団1では、雌雄が逆の系統に属する交配集団2に比較して高率(1.7%vs16.0%)に城崎系であるグループが作出された。集団1では、種雄牛による差が大きく、奥秀、城富、城和、勘高や大勘で城崎系産子を生産する割合が高かったが、光城波は亜系の新たなグループを形成した。集団2では、城崎系を生産する母牛が6頭程度に限られ、中土井系の種雄牛を交配することで城崎系の産子を生産する可能性は低かった。
兵庫県立農林水産技術総合センター, 2003年, 兵庫県立農林水産技術センター研究報告［畜産編］, 39:16-21 (39), 16 - 21, 日本語研究論文（学術雑誌）
- 日本動物遺伝育種学会, 2003年, 動物遺伝育種研究, 30:17-20. (2), 17 - 20, 日本語
研究論文（学術雑誌）
- Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle
Complete sequences of mitochondrial (mt) genomes of eight Japanese Black cattle were determined to investigate the relationships between mt deoxyribonucleic acid (DNA) displacement loop (D-loop) types and other mtDNA regions and to identify the variation in the coding region that may influence the economic traits. The survey of mitochondrial sequences in the encoding region revealed 14 substitutions including six antonymous substitutions and one in 16S ribosomal ribonucleic acid (rRNA). Three methods of polymorphic DNA analyses (polymerase chain reaction [PCR]-restriction fragment length polymorphism [RFLP], mismatch PCR-RFLP, PCR-single-strand conformation polymorphism [SSCP]) were performed on these seven candidate substitutions (base pair [bp] 2,232, 12,158, 12,908, 13,310, 14,122, 14,140, and 14,565) for 202 Japanese Black cattle. The substitution of by 13,310 was observed in all samples, but not in the reference sequence, indicating that this is a minor substitution or a sequencing mistake in the reference sequence. The substitutions at by 14,122, 14,140, and 14,565 were observed in only a few samples, suggesting that these were also minor substitutions. The substitutions at by 2,232 (16S rRNA), 12,158, and 12,908 (reduced nicotinamide adenine dinucleotide-ubiquinone oxidoreductase chain-5) were closely related to mitochondrial D-loop types that have previously been related to differences in the carcass traits of Japanese Black cattle. Evaluation of the effects on six carcass traits with mixed model procedures suggests that the by 2,232 substitution affects longissimus muscle area and beef marbling score. The substitution at by 2,232 is a strong candidate for the mitochondrial effect on meat quality.
AMER SOC ANIMAL SCIENCE, 2003年01月, JOURNAL OF ANIMAL SCIENCE, 81 (1), 68 - 73, 英語研究論文（学術雑誌）
- 2003年, Animal Genetics, 未記入, 英語Genetic parameters for reproductive performances of breeding cows and carcass traits of fattening animals in Japanese Black (Wagyu) cattle.
研究論文（学術雑誌）
- 2003年, Livest. Prod. Sci., 未記入, 215 - 220, 英語Differences in Stearoyl-CoA Desaturase mRNA levels between Japanese Black and Holstein cattle.
研究論文（学術雑誌）
- Heritability and repeatability estimates for reproductive traits of Japanese black cows
Reproductive data collected from more than 20,000 Japanese Black cows of Hyogo and Shimane Prefectures were analyzed. Averages of age at first calving, gestation length, days open and calving interval were 25.1 mo, 289 d, 112 d and 401 d, respectively. Variance components were obtained by REML procedure and the heritability estimate of age at first calving was 0.22. In gestation length the heritability estimate was 0.40 and no permanent environmental effect was estimated. Estimated variance components of calving interval were similar to those of days open and the heritability and repeatability of calving interval were 0.0 and 0.09, respectively. Random farm effects accounted for approximately 10% of phenotypic variations in all traits. Genetic and farm correlations between age at first calving and calving interval were 0.27 and 0.39, respectively. It was found that temporary environment was an important source of variation for calving intervals of Japanese Black.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, 2002年12月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 15 (12), 1680 - 1685, 英語[査読有り]
研究論文（学術雑誌）
Genetic relationships among carcass traits and between reproductive or growth traits and carcass traits were estimated for Japanese Black using REML procedure. Among carcass traits a low but favorable genetic correlation (-0.04) was obtained between two c
神戸大学, 2002年02月25日, 神戸大学農学部学術報告, 26, 49 - 54, 日本語育種価予測値による選抜が普及するにつれ,近交度を抑制し改良量を最大にする交配計画の重要性はますます大きくなることが予想される.しかしながら,次世代の近交度のみを考慮すれば,血縁関係の薄い特定の種雄牛に供用が集中し,中•長期の育種改良に弊害が生じる危険性がある.したがって本報では,現実の集団の育種価予測値(PBV)を用い,集団の近交度,種雄牛の供用頻度および個々の後代の近交係数に制限を加えた交配計画の最適化を遺伝的アルゴリズム(GA)により試みた.最適化の対象とした牛群は,種雄牛15頭および繁殖雌牛574頭からなっており,この牛群の繁殖雌牛に対して審査得点のPBV(JS-Data)あるいは脂肪交雑評点のPBV(BMS-Data)により400頭の選抜を行った.交配計画により改良対象とした形質は枝肉5形質,審査得点ならびに繁殖能力2形質を総合した遺伝的水準(AGM)とした.ただし,枝肉形質以外には重み付けを行わず,枝肉形質を主体とした交配の影響を考察するにとどめた.両データで制限をまったく加えない交配計画を探索したところ,1頭の種雄牛に供用が集中したが,種雄牛の供用頻度を0.30に制限した場合でも,制限を加えない場合と比較してAGMの改良量はJS-DataおよびBMS-Dataでそれぞれ31.3%および35.6%小さくなる程度にとどまった.交配計画に起因する脂肪交雑評点の改良量はJS-Dataの方が大きかったものの,選抜に起因する改良量ではBMS-Dataの方が大きく,脂肪交雑評点の合計の改良量ではBMS-Dadaが28.3%大きな改良量を示した.また,GAは設定したすべての制限値の下で交配計画の決定に成功し,3つの制限に柔軟に対応できることが認められた.
Japanese Society of Animal Science, 1998年04月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 69 (4), 333 - 340, 英語Japanese Black fattening steers were used to examine relationships between carcass traits and mitochondria displacement loop (D-loop) variations. The D-loop region of Japanese Black cattle was sequenced and revealed 26 mitochondrial haplotypes defined by 25 polymorphic sites. The haplotypes were classified into five mitochondrial types (type 1 to 5) using the unweighted pair-group method with arithmetic means. Carcass weight, longissimus muscle area (LMA), rib thickness, subcutaneous fat thickness, yield estimate, and beef marbling score (BMS) were compared among five mitochondrial types with BLUP procedures. Significant differences between mitochondria types were detected for LMA and BMS. Difference (P <.05) was observed between mitochondrial types 2 and 4 for LMA. There was a highly significant difference (P <.01) in EMS between types 2 and 4. Difference (P <.05) was also found between types 1 and 4 on BMS. These results suggest that cytoplasmic genetic effects are important sources of variation for carcass traits in Japanese Black cattle.
AMER SOC ANIMAL SCIENCE, 1998年01月, JOURNAL OF ANIMAL SCIENCE, 76 (1), 36 - 41, 英語[査読有り]
研究論文（学術雑誌）
黒毛和種の比形質およびそれらの成分形質に関して,線形性および正規性を仮定して遺伝的パラメータ値を算出し,比形質の表型価に基づく個体選抜を想定して,比および成分形質の遺伝的改良量を予測する上での比形質に関する当該パラメータ値の有効性について検討を加えた.対象とした比形質は,TDN効率(TDNE;増体量(WG)/TDN摂取量(TDNI))および粗飼料摂取率(RIR;粗飼料摂取量(RI)/総飼料摂取量(TFI))である.(共)分散成分の推定は,一分集団における662頭の直接検定牛の記録を用いて,縮約化個体モデル-REML法により行った.なお,検討に当たっては,成分形質に関するパラメータの推定値は現集団での真値に等しいと仮定した.TDNEに関する選抜により,線形性•正規性の仮定下では,WGおよびTDNIの集団平均はそれぞれ増加および減少すると予測され,真に期待される遺伝的変化の方向と一致したが,変化の量は,WGで過大評価されTDNIでは過小評価された.RIRに関する選抜を実施すると,実際にはRIおよびTFIの平均はともに低下すると期待されたが,線形性•正規性の仮定下では,RIの平均は上昇しTFIのそれは低下すると予測された.所与の仮定下で,比のテイラー展開に基づき定義されたパラメータ値を用いた場合にも,比および成分形質について実際に生じると期待される遺伝的変化は適切に説明されなかった.よって,TDNEおよびRIRについて線形性および正規性を仮定して個体モデルにより算出されたパラメータの値は,これらの比形質に対する選抜の結果を予測する上で的確な情報でない可能性が示唆される.なお,RIRに関する選抜により,両成分形質の集団平均がともに低下するとすれば,飼料摂取能力の最適化の観点から,望ましい現象でない可能性がある点に留意を要する.
Japanese Society of Animal Science, 1996年11月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 67 (11), 949 - 955, 英語これまでの選抜反応の予測式は,すべての個体が同一の正確度を持つとの仮定によって導かれてきているが,BLUP法で予測される育種価は各個体が記録の有無や血縁個体からの情報量により異なった正確度を持つ.したがって本報告では,個体ごとに選抜の正確度が異なる状況を想定し,直接ならびに間接選抜反応を予測する3種の予測式をシミュレーションにより比較した.予測式としては,1) 従来の予測式の選抜の正確度を,育種価予測値の正確度の平均値に置き換えた予測式(AA),2) 選抜対象集団を育種価予測値の正確度によりグループ化し,それぞれのグループから重みづけした改良量を算出する予測式(GA)および3) 育種価予測値の選抜差(SP)の3種を用いた.直接選抜反応を予測する場合,予測式間の差は小さかったが,AAの間接選抜反応は真値を大きく過小評価した.一般に本報告でシミュレートした集団のように,育種価予測値とその正確度に関連性が認められない状況では,間接選抜反応においてSPはGAより優れた予測精度を示すことが認められた.
Japanese Society of Animal Science, 1996年11月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 67 (11), 930 - 940, 英語家畜改良センター岡崎牧場において改良量に基づく選抜指数式により1989年から1994年にかけて選抜が実施されてきた卵用鶏3系統を用いて,期待される選抜反応と実際に達成された選抜反応との比較検討を行った.3系統とも,252日齢での卵重(EW)と256日齢の体重(BW)を変化させず,初産から280日齢までの産卵率(EPB)を5%上昇させ,さらに1系統では初産日齢(SM)を5日短縮する改良目標が設定された.選抜指標としては,EW,BW,SMおよび169日齢から280日齢の産卵率(EPA)が用いられた.選抜指数式の作成には3系統ともに同一の遺伝的パラメーターが仮定され,同一の指数により5世代にわたる選抜が実施された.達成された選抜反応は,多形質REML法により推定した遺伝的パラメーターをもとに,多形質アニマルモデルBLUP法を用いて育種価を予測し,生年ごとの育種価予測値平均の世代への回帰係数より算出した.3系統ともに,BWを除き,選抜反応の方向は設定した改良目標と一致していた.しかし,体重の選抜反応は2系統では有意な減少傾向を示し,これはBWとEPAあるいはEPB間には遺伝的関連がないと仮定した指数を用いているのに対して,REMLでは小さいながらも負の遺伝相関が存在したことに起因すると考えられた.EPBの改良量に関しては,仮定したパラメーターと各系統のREML推定値の差異を反映して,ほぼ理論値に一致する系統から30%程度の系統までと異なる反応量を示した.したがって,REML法による各系統固有の遺伝的パラメーター推定値を用いた選抜指数式による選抜が改良の方向性や改良量に関して確実な効果をもたらすものと考えられた.
Japanese Society of Animal Science, 1996年11月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 67 (11), 941 - 948, 英語予測育種価を用いた選抜は,集団の近交度を急激に上昇させる危険性をともなうため,遺伝的アルゴリズム(GAs)を用いて近交度に制限を加えた交配計画の最適化を試みた.GAsとは,「染色体」が「交叉」し「遺伝子」が「突然変異」を起こしながら「適応度」の高い個体が生き残っていく進化の過程を模倣して最適解を探索するアルゴリズムである.近交度に制限を加えない状況でGAの探索能力を検討した結果,染色体数および交叉回数のオペレーターの影響はほとんど認められなかった.しかし,突然変異率が減少するにつれ,GAの探索能力は向上した.近交に制限を加えて交配計画の最適化を試みたところ,過剰な近交度を示した染色体,すなわち交配計画に適切なペナルティーを与えてやれば,GAにより妥当な交配計画が探索された.ペナルティーの大きさはGAが探索できる解空間の大きさに依存するなど,GAの実際の適用には,オペレーターの決定にさらなる研究が必要であるが,有効な方法となりうることが明らかになった.
Japanese Society of Animal Science, 1996年10月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 67 (10), 835 - 843, 英語黒毛和種繁殖雌牛の登録審査時に測定されている諸形質,ならびに繁殖能力と枝肉成績との遺伝的関連性を多形質アニマルモデルによるREML法により推定した.分析形質は,広島県下で収集された外貌審査による減率4項目および審査得点,体測定値3形質,繁殖能力2形質(初産月齢および分娩間隔)ならびに枝肉成績5形質である.外貌審査形質,体測定値および枝肉成績の遺伝率は中位から高めで推定されたが,繁殖能力の遺伝率は非常に低い値であった.外貌審査形質と体測定値間の遺伝相関は,体重体高比を加えないモデルでは高く推定された.初産月齢は,外貌審査形質,体側定値および枝肉成績と好ましい遺伝的関連性を示したが,分娩間隔と脂肪交雑評点の間には正の好ましくない関係が認められた.今後,枝肉成績に対する選抜圧が強くなることが予想されるため,分娩間隔の増加に注意する必要性が示唆された.
Japanese Society of Animal Science, 1996年06月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 67 (6), 511 - 518, 英語- Genetic relationships between performance test traits and field carcass traits in Japanese Black cattle
Genetic relationships between body measurements and growth traits at performance testing of bull calves and field carcass traits in Japanese Black cattle were estimated using a two-trait sire and maternal grandsire model with REML procedure. Covariances between performance test and carcass traits were obtained from across environment analyses. Performance test traits included wither height, chest girth, chest depth, thurl width, body weight, and daily gain. For field carcass traits carcass weight, longissimus muscle area, rib thickness, subcutaneous fat thickness, yield estimate, and beef marbling score were measured on 8329 steers and heifers. Heritabilities for performance test traits ranged from 0.13 to 0.36, and for carcass traits from 0.39 to 0.55. The basis of selection at performance testing, daily gain, did not correlate well with carcass beef marbling score, the breeding goal, indicating that genetically superior bull calves in marbling may be culled at the end of testing. Chest girth at the middle and the end of testing correlated well in the desired directions with all carcass traits. It is possible to improve total merit of the carcass by introducing chest girth into performance testing.
ELSEVIER SCIENCE BV, 1995年12月, LIVESTOCK PRODUCTION SCIENCE, 44 (3), 199 - 205, 英語[査読有り]
研究論文（学術雑誌）
黒毛和種の直接検定時における超音波推定値と,肥育現場から収集された枝肉形質間の遺伝的関連性を推定した.超音波測定には鹿児島県畜産試験場において直接検定を受検した154頭の雄牛を用い,直接検定の開始時および終了時に第6-7肋骨間ロース芯面積(7-REAu),第12-13肋骨間ロース芯面積(13-REAu),バラの厚さ(RTu),皮下脂肪厚(SFTu),筋間脂肪厚(IMFTu)および脂肪交雑評点(BMSu)を測定した.現場枝肉形質は同県下から収集された4,725頭の枝肉格付成績のうち枝肉重量(CWc),ロース芯面積(REAc),皮下脂肪厚(SFTc),バラの厚さ(RTc),推定歩留(YEc)および脂肪交雑評点(BMSc)の6形質を用い,2形質下種雄牛•母方祖父モデルによるREML法により,分散•共分散成分の推定を行なった.検定終了時の遺伝率は,7-REAuで0.20, SFTuで0.58およびBMSuで0.15, CWcで0.25, REAcで0.52, SFTcで0.55およびBMScで0.56と推定された.REAuとREAcとの遺伝相関は0.52から0.71と高い正の係数を示したが,SFTに関しては両者間に負,またBMSに関しては直接検定開始時には正,終了時には負の遺伝相関が認あられた.以上のように検定期間中を通じて一定の遺伝的関連性を示さない形質も認められたが,直接検定時に超音波測定を導入することにより,現場枝肉形質のロース芯面積を改良できる可能性が示された.
Japanese Society of Animal Science, 1995年01月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 66 (1), 7 - 15, 英語
MISC
- 電子情報通信学会, 2019年07月10日, 電子情報通信学会技術研究報告 = IEICE technical report : 信学技報, 119 (106), 139 - 144, 日本語依頼講演 放牧牛インタラクション分析のための省電力無線による位置トラッキング (高信頼制御通信)
- 2019年03月, 電子情報通信学会総合大会予稿集, B-18-6, 日本語省電力無線タグ受信電力に基づく時系列を考慮した深層学習による屋外位置推定
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2019年03月, 電子情報通信学会総合大会予稿集, B-18-5, 日本語省電力無線タグのモデルベース受信電力推定データを用いた深層学習に基づく屋外位置推定方式に関する一検討
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2019年, 肉用牛研究会報, (106)遺伝的多様性確保に向けた新たな改良指標の設定
- 2019年, 日本畜産学会大会講演要旨, 125th複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
- 2019年, 日本畜産学会大会講演要旨, 126th兵庫県産黒毛和種の枝肉単価に対するモノ不飽和脂肪酸割合及び細かさ指数の影響
- 2019年, 日本畜産学会大会講演要旨, 126th但馬牛の系統分類が長期的な遺伝的多様性に与える効果
- 2018年, 日本畜産学会大会講演要旨, 124th兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討
- 2018年, 日本畜産学会大会講演要旨, 124thウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
- 畜産技術協会, 2017年02月, 畜産技術 = Livestock technology, (741), 5 - 8, 日本語肥育後期の黒毛和種における腰最長筋の脂肪酸組成の変化
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, 英語Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.
研究発表ペーパー・要旨（国際会議）
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, 英語
研究発表ペーパー・要旨（国際会議）
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, 英語
研究発表ペーパー・要旨（国際会議）
- 2015年03月28日, 日本畜産学会大会講演要旨, 119th, 162, 日本語胆馬牛における胸最長筋,背半棘筋及び僧帽筋の画像解析形質に関する遺伝的パラメータの推定
- 2015年03月28日, 日本畜産学会大会講演要旨, 119th, 163, 日本語兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の有効性
- 2013年01月24日, 肉用牛研究会報, 94 (94), 51 - 52, 日本語但馬系黒毛和種における在胎日数の年次推移とそれに影響を及ぼす要因の解明
- 日本動物遺伝育種学会, 2012年12月01日, 動物遺伝育種研究, 40 (2), 78 - 78, 日本語ウシFABP4遺伝子のプロモーター領域に対する多型探索
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 73, 日本語岐阜県黒毛和種集団におけるUTS2R遺伝子内多型の脂肪酸組成に対する効果
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 85, 日本語黒毛和種におけるLEP遺伝子内多型の脂肪酸組成に対する効果
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 72, 日本語黒毛和種におけるFASN遺伝子内多型の脂肪酸組成に対する効果
- 2012年01月28日, 肉用牛研究会報, 92, 64 - 64, 日本語大容量データによる黒毛和種雌牛の繁殖形質についての遺伝率および反復率の推定
- 2011年12月01日, 動物遺伝育種研究, 39 (2), 133, 日本語ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索
The genetic evaluation of economically important traits utilizes estimates of genetic variability, which are represented by heritability. This review summarizes the published heritabilities of traits estimated in Wagyu cattle. Two different mean heritabilities, unweighted and weighted by standard errors, were calculated. In Japanese Black cattle, the average unweighted and weighted direct heritabilities of birth weight were 0.35 and 0.28, respectively, whereas the respective maternal heritabilities were 0.17 and 0.07. The mean unweighted heritability of calf market weight was estimated to be 0.30 in Japanese Black cattle. The mean unweighted heritability of daily gain during performance testing was 0.29 in Japanese Black and 0.40 in Japanese Shorthorn cattle. In Japanese Black cattle, the unweighted mean heritability was 0.48 for carcass weight, 0.46 for rib-eye area, 0.38 for rib thickness, 0.39 for subcutaneous fat thickness, and 0.55 for marbling. The mean weighted heritability of the calving interval was low, and estimated to be 0.05. In general, the heritabilities estimated in Wagyu cattle were similar to those estimated in other beef breeds.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (3), 367 - 373, 英語[査読有り]
書評論文,書評,文献紹介等
- 鳥取県農林水産部農林総合研究所畜産試験場, 2008年12月, 鳥取県農林水産部農林総合研究所畜産試験場研究報告, (36), 14 - 21, 日本語鳥取和牛肉の脂肪酸組成割合に関する遺伝的パラメータの推定
- 日本生物工学会, 2008年, 生物工学会誌 : seibutsu-kogaku kaishi, 86 (8), 410 - 411, 日本語科学を用いた美味しさの追及「神戸大学ビーフ」(大学発!美味しいバイオ)
- 2005年12月20日, 肉用牛研究会報, 80, 7 - 9, 日本語種雄牛造成や交配種雄牛助言サービスソフト「MSAS」の利用が但馬牛集団に及ぼす影響
Performance test results of 3250 sire candidates were used to estimate the genetic parameters of growth and feed utilization traits in Japanese Black cattle. Growth traits analyzed were six body measurements at the end of the performance test and daily gain (DG) during the test. Feed utilization traits were intakes and conversions of concentrate, roughage, digestible crude protein and total digestible nutrient (TDN). Genetic (co)variance components were estimated by the restricted maximum likelihood procedure using an expectation maximization algorithm under the two-trait animal model. Heritabilities for growth traits ranged from 0.40 to 0.70 and for feed utilization traits from 0.21 to 0.74. Genetic correlations of DG were positive with feed intake (0.15-0.77) and negative with feed conversions (-0.63 to -0.30). These relationships indicate that the selection based on DG improves feed efficiency but it simultaneously increases feed intake. Feed conversions showed genetic correlations ranging from -0.09 to 0.03 with total available energy consumption, TDN intake. Thus the results suggested that feed conversions were not efficient selection criteria to decrease TDN intake and to improve comprehensive feed utilization ability.
2005年04月, Animal Science Journal, 76 (2), 115 - 119, 英語その他
- 2003年01月22日, 肉用牛研究会報, 74, 7 - 9, 日本語交配種雄牛助言サービスソフト(MSAS)の繁殖現場での利用効果と問題点
- 但馬牛集団の血縁係数による系統分類の試み
血縁係数を利用したクラスター分析により但馬牛集団の系統分類を試み、現存集団の遺伝的構成を把握し、系統の再構築の可能性を検討した。とくに、今回の分析で規模が小さな集団の増頭を目的として、仮想集団を作出した場合の系統分類についても検討した。1.供用中集団のデンドログラムを血縁係数0.10で切断し、7グループに分類した。2.各グループ内及びグループ間の平均血縁係数は、グループ1、2、3と5では全て0.3以上であった。グループ4も概ね0.3で上記グループとの血縁が高かった。グループ6と7が、他グループと異なりグループ間の血縁係数が低かった。3.各グループの育種価評価値の平均値を比較するとグループ毎に産肉あるいは種牛能力の特徴が明らかになった。4.600頭の仮想雌牛群と待機雄牛の後代を加え系統分類を実施した。その結果、中土井系繁殖雌牛に城崎系種雄牛を交配するシミュレーション集団1では、雌雄が逆の系統に属する交配集団2に比較して高率(1.7%vs16.0%)に城崎系であるグループが作出された。集団1では、種雄牛による差が大きく、奥秀、城富、城和、勘高や大勘で城崎系産子を生産する割合が高かったが、光城波は亜系の新たなグループを形成した。集団2では、城崎系を生産する母牛が6頭程度に限られ、中土井系の種雄牛を交配することで城崎系の産子を生産する可能性は低かった。
兵庫県立農林水産技術総合センター, 2003年, 兵庫県立農林水産技術総合センター研究報告〔畜産編〕, 39,pp.16-21 (39), 16 - 21, 日本語その他
- 畜産技術協会, 2001年08月, 畜産技術, (555), 17 - 20, 日本語予測育種価に基づく黒毛和種の遺伝的改良
- 2000年12月25日, 肉用牛研究会報, 70, 27 - 28, 日本語但馬牛における生後270日齢までの平均発育曲線の推定
- 日本畜産学会, 1999年09月25日, Animal science journal, 70 (5), 268 - 272, 英語Property of Difference between Two Predicted Breeding Values from BLUP Methodology
- 1999年09月25日, 日本畜産學會報 = The Japanese journal of zootechnical science, 70 (5), 268 - 272, 英語Property of Difference between Two Predicted Breeding Values from BLUP Methodology
- 日本万国家禽学会, 1996年05月25日, 日本家禽学会誌, 33 (3), 185 - 192, 英語多形質アニマルモデルREML法による卵用鶏の遺伝的パラメーターの推定
- 日本万国家禽学会, 1995年11月25日, 日本家禽学会誌, 32 (6), 379 - 386, 英語アニマルモデルBLUP法による肉用鶏選抜系統の遺伝的趨勢
- 日本万国家禽学会, 1995年07月25日, 日本家禽学会誌, 32 (4), 257 - 266, 英語REML法による肉用鶏選抜系統の遺伝的パラメーターの推定
- 広島県立畜産試験場, 1995年03月, 広島県立畜産試験場研究報告, (10), p55 - 62, 日本語肥育農家を変量効果としたときの枝肉形質の遺伝的評価への影響
書籍等出版物
- 分担執筆, 北隆館, 2020年01月アグリバイオ 特集「和牛の育種改良」
- その他, 丸善プラネット, 2018年03月, 日本語兵庫の和牛 但馬牛－育種価評価による改良の推進
学術書
- その他, 朝倉書店, 2017年03月, 日本語動物遺伝育種学－選抜と選抜育種
教科書・概説・概論
- その他, 誠文堂新光社, 2016年10月, 日本語エシカルな農業－未来の但馬牛のために今すべきこと
一般書・啓蒙書
- その他, 講談社, 2014年07月, 日本語, ISBN: 9784061537392最新畜産ハンドブック
その他
- 共著, 化学同人, 2011年03月, 日本語生物統計学
教科書・概説・概論
講演・口頭発表等
- 和牛育種組合連合会第60回通常総会
[招待有り]
- 湯村温泉但馬ビーフ流通振興協議会総会
[招待有り]
- 平成3年度日本獣医師会獣医学術学会年次大会シンポジウム
[招待有り]
- WagyuEdge Conference, 2021年04月28日Genetic improvement of Japanese Black / Overview of Japanese Breeding Programs
[招待有り]
- 日本畜産学会第126回大会, 2019年09月但馬牛の系統分類が長期的な遺伝的多様性に与える効果
口頭発表（一般）
- 日本畜産学会第126回大会, 2019年09月兵庫県産黒毛和種の枝肉単価に対するモノ不飽和脂肪酸割合及び細かさ指数の影響
口頭発表（一般）
- 34th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Validation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic Circuit
ポスター発表
- 34th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations
ポスター発表
- 第125回日本畜産学会大会, 2019年03月, 日本語, 日本畜産学会, 相模原, 国内会議複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
口頭発表（一般）
- 電子情報通信学会総合大会, 2019年03月, 日本語, 電子情報通信学会, 新宿区, 国内会議省電力無線タグ受信電力に基づく時系列を考慮した深層学習による屋外位置推定
口頭発表（一般）
- 電子情報通信学会総合大会, 2019年03月, 日本語, 電子情報通信学会, 新宿区, 国内会議省電力無線タグのモデルベース受信電力推定データを用いた深層学習に基づく屋外位置推定方式に関する一検討
口頭発表（一般）
- 第56回肉用牛研究会愛媛大会, 2018年11月, 日本語, 国内会議遺伝的多様性確保に向けた新たな改良指標の設定
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2018年09月, 日本語, 京都, 国内会議兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出
口頭発表（一般）
- 日本動物遺伝育種学会第18回大会, 2018年09月, 日本語, 国内会議黒毛和種で発生する白斑と枝肉形質との関連性の調査
ポスター発表
- 第18回日本動物遺伝育種学会大会, 2018年09月, 日本語, 京都, 国内会議ゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索
口頭発表（一般）
- 平成30年度 第68回関西畜産学会大会, 2018年09月, 日本語, 関西畜産学会, 国内会議IoTを活用した放牧牛のインタラクション分析に基づく飼養管理技術の開発
口頭発表（一般）
- PAG Asia 2018, 2018年05月, 英語, Scherago International, Seoul, Korea, 国際会議Effect of SLC27A6 gene K81M polymorphism on fat percentage in rib-eye area in Japanese Black cattle
ポスター発表
- PAG Asia 2018, 2018年05月, 英語, Scherago International, Seoul, Korea, 国際会議Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data
ポスター発表
- 第124回日本畜産学会大会, 2018年03月, 日本語, 日本畜産学会, 東京, 国内会議兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討
口頭発表（一般）
- 第124回日本畜産学会大会, 2018年03月, 日本語, 日本畜産学会, 東京, 国内会議ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2017年11月, 日本語, 動物遺伝育種学会, 厚木, 国内会議兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2017年11月, 日本語, 動物遺伝育種学会, 厚木, 国内会議複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討
口頭発表（一般）
- 第67回関西畜産学会大会, 2017年09月, 日本語, 関西畜産学会, 大阪, 国内会議ゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索
口頭発表（一般）
- 電子情報通信学会コミュニケーションクォリティ研究会研究会, 2017年07月, 日本語, 電子情報通信学会, 神戸市, 国内会議無線タグによる和牛の個体識別・トラッキングに関する取り組み
口頭発表（招待・特別）
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Genome-wide association study revealed a candidate region for beef marbling on BTA7 in Japanese Black cattle
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Effects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle
ポスター発表
- 日本畜産学会第122回大会, 2017年03月, 日本語, 神戸大学, 国内会議黒毛和種の損徴に関する遺伝育種学的研究
口頭発表（一般）
- 日本動物遺伝育種学会第17回大会, 2016年11月, 日本語, 国内会議鹿児島県産黒毛和種で発生する白斑に関する遺伝育種学的研究
ポスター発表
- 第17回日本動物遺伝育種学会大会, 2016年11月, 日本語, 日本動物遺伝育種学会, 名古屋, 国内会議黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連
口頭発表（一般）
- 日本暖地畜産学会第9回山口大会, 2016年10月, 日本語, 国内会議鹿児島県産黒毛和種における死産と母牛の審査時形質との関連性
口頭発表（一般）
- 平成28年 電気学会 電子・情報・システム部門大会, 2016年08月, 日本語, 国内会議ステレオカメラを用いた肥育牛の体重予測
口頭発表（一般）
- Proceedings of 17th AAAP Animal Science Congress, 2016年08月, 英語, 国際会議Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima
ポスター発表
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle
ポスター発表
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black
ポスター発表
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Genome-wide association study identifies a QTL for fat percentage in rib-eye area on BTA10 in Japanese Black cattle
ポスター発表
- 第121回日本畜産学会大会, 2016年03月, 日本語, 日本畜産学会, 東京, 国内会議ウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索
ポスター発表
- 日本動物遺伝育種学会第16回大会, 2015年11月, 日本語, 神戸, 国内会議兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の長期的な有効性
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議GWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索
ポスター発表
- 第62回情報システム研究会, 2015年05月, 日本語, 神戸大学, 国内会議放牧場の俯瞰動画データを用いた繁殖牛のインタラクション検出
口頭発表（一般）
- 第62回情報システム研究会, 2015年05月, 日本語, 神戸大学, 国内会議GPSを利用した放牧牛のインタラクション情報の取得とその分析
口頭発表（一般）
- 日本畜産学会, 2015年03月, 日本語, 宇都宮大学, 国内会議兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の有効性
口頭発表（一般）
- 日本畜産学会第119回大会, 2015年03月, 日本語, 宇都宮市, 国内会議但馬牛における胸最長筋，背半棘筋及び僧帽筋の画像解析形質に関する遺伝的パラメータの推定
口頭発表（一般）
- 日本畜産学会第119回大会, 2015年03月, 日本語, 宇都宮市, 国内会議黒毛和種肥育牛1047頭を用いたオレイン酸割合ゲノム育種価評価の試み
口頭発表（一般）
- 第118回日本畜産学会大会, 2015年03月, 日本語, 日本畜産学会, 宇都宮, 国内会議黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果
口頭発表（一般）
- 日本畜産学会, 2015年03月, 日本語, 宇都宮大学, 国内会議黒毛和種における内臓疾病に関連した経済的損失
口頭発表（一般）
- 日本畜産学会, 2015年03月, 日本語, 宇都宮大学, 国内会議見島ウシの集団構造に関する基礎的研究
口頭発表（一般）
- 平成26年度現場後代検定合同調査会シンポジウム, 2015年02月, 日本語, 神戸市, 国内会議肥育後期の黒毛和種における 不飽和脂肪酸の体内変化について
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 第15回日本動物遺伝育種学会大会, 2014年11月, 日本語, 日本動物遺伝育種学会, 和光, 国内会議ホルスタイン種におけるFASN-g.841 及びg.16024 多型の乳形質に対する効果
ポスター発表
- 2014年度農業施設学会大会シンポジウム, 2014年08月, 日本語, 神戸市, 国内会議牛肉ブランドの現状と今後の方向性
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議LPL gene polymorphisms are associated with fatty acid composition in Japanese Black
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議Genome wide association study revealed candidate regions for calving interval in Japanese black cattle
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議A genome wide association study for beef marbling and fatty acid composition in Japanese Black cattle using pooled DNA
口頭発表（一般）
- 日本畜産学会第118回大会, 2014年03月, 日本語, つくば市, 国内会議鹿児島県産黒毛和種の枝肉における瑕疵間の遺伝相関の推定
口頭発表（一般）
- 日本畜産学会第118回大会, 2014年03月, 日本語, つくば市, 国内会議黒毛和種肥育牛を訓練集団としたオレイン酸割合ゲノム育種価評価の試み
ポスター発表
- 日本畜産学会第118回大会, 2014年03月, 英語, つくば市, 国内会議Searching for causal structures among internal diseases and carcass traits in Japanese Black cattle
ポスター発表
- 第14回日本動物遺伝育種学会大会, 2013年10月, 日本語, 日本動物遺伝育種学会, 東京, 国内会議黒毛和種におけるLEP遺伝子プロモーター領域に対する多型探索
ポスター発表
- 第116回日本畜産学会大会, 2013年03月, 日本語, 日本畜産学会, 広島, 国内会議ウシ脂肪酸組成に対するFASN 遺伝子内多型の効果
口頭発表（一般）
- 日本畜産学会第117回大会, 2013年, 日本語, 国内会議兵庫系黒毛和種における育種集団毎の遺伝的多様性評価
口頭発表（一般）
- 日本畜産学会第117回大会, 2013年, 日本語, 国内会議但馬牛における枝肉画像解析形質の遺伝的パラメータの推定
口頭発表（一般）
- 2013年度日本暖地畜産学会, 2013年, 日本語, 国内会議鹿児島県産黒毛和種を用いた瑕疵と枝肉6形質の遺伝相関及び表型相関
口頭発表（一般）
- 日本畜産学会第117回大会, 2013年, 日本語, 国内会議鹿児島県産黒毛和種の枝肉で発生する瑕疵に関する遺伝的パラメータの推定
口頭発表（一般）
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議兵庫県黒毛和種集団における系統内交配による遺伝的分化の可能性
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議黒毛和種におけるLEP 遺伝子内多型の脂肪酸組成に対する効果
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議黒毛和種におけるFASN 遺伝子内多型の脂肪酸組成に対する効果
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議岐阜県黒毛和種集団におけるUTS2R 遺伝子内多型の脂肪酸組成に対する効果
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 仙台, 国内会議ウシFABP4 遺伝子のプロモーター領域に対する多型探索
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議The SNPs in the ACACA gene effective on fatty acid composition in Holstein milk
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議The SNP in the promoter region of the bovine ELOVL5 gene is effective on subcutaneous fat thickness
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議Identification of quantitative trait loci affecting economic traits based on divergently selected regions between Japanese Black and Holstein cattle
ポスター発表
- 第2回但馬牛改良セミナー, 2012年, 日本語, 国内会議和牛の改良と但馬牛
公開講演，セミナー，チュートリアル，講習，講義等
- 第50回肉用牛研究会, 2012年, 日本語, 国内会議但馬系黒毛和種における在胎日数の年次推移とそれに影響を及ぼす要因の解明
口頭発表（一般）
- 第62回関西畜産学会大会, 2012年, 日本語, 国内会議黒毛和種の肥育後期における胸最長筋および腰最長筋の脂肪酸組成の経時的変化
口頭発表（一般）
- 神戸大学農学研究科公開講座, 2012年, 日本語, 国内会議プロローグ ～方舟に乗せるべき遺伝資源～
公開講演，セミナー，チュートリアル，講習，講義等
- 第12回日本動物遺伝育種学会大会, 2011年11月, 日本語, 日本動物遺伝育種学会, 広島, 国内会議黒毛和種およびホルスタインにおけるUTS2R遺伝子多型の脂肪酸組成に対する効果
ポスター発表
- 第12回日本動物遺伝育種学会大会, 2011年11月, 日本語, 日本動物遺伝育種学会, 広島, 国内会議ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索
ポスター発表
- 第49回肉用牛研究会, 2011年, 日本語, 国内会議大容量データによる黒毛和種雌牛の繁殖形質についての遺伝率および反復率の推定
口頭発表（一般）
- 大阪府立大学「食」フォーラム, 2011年, 日本語, 国内会議神戸大学農学部における食の教育
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 日本畜産学会第114回大会, 2011年, 日本語, 国内会議黒毛和種の肥育後期における腰最長筋の筋肉内脂肪および脂肪酸組成の経時的変化
ポスター発表
- 第11回日本動物遺伝育種学会大会, 2010年10月, 日本語, 日本動物遺伝育種学会, 白河, 国内会議黒毛和種集団におけるsliding window approachを利用した枝肉形質関連遺伝子座の探索
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Polymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattle
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Development of discrimination markers between Japanese domestic and imported beef based on a bovine 50K SNP array
ポスター発表
- 第112回日本畜産学会大会, 2010年03月, 日本語, 日本畜産学会, 明治大学, 国内会議黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果
口頭発表（一般）
- 平成22年度問題別研究会, 2010年, 日本語, 国内会議和牛における育種の現状と展開
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 平成22年度関西畜産学会大会, 2010年, 日本語, 国内会議黒毛和種における脂肪酸組成の遺伝率推定に関する検討
口頭発表（一般）
- 嶺南大学校(韓国)農業マイスター大学シンポジウム, 2010年, 日本語, 国際会議Wagyu
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 9th World Congress of Genetics Applied to Livestock Production, 2010年, 英語, 国際会議Sampling method for estimating neutral allele frequency in a pedigreed population
ポスター発表
- 第10回日本動物遺伝育種学会大会, 2009年11月, 日本語, 日本動物遺伝育種学会, 群馬会館, 国内会議ホルスタイン種におけるFASN遺伝子の多型探索及び乳形質の効果
ポスター発表
- 第111回日本畜産学会大会, 2009年09月, 日本語, 日本畜産学会, 琉球大学, 国内会議ホルスタイン種における枝肉形質に関る遺伝子多型の効果
口頭発表（一般）
- 神戸大学全学統一公開講座, 2009年, 日本語, 国内会議和牛の来た道、進む道
公開講演，セミナー，チュートリアル，講習，講義等
- 日本畜産学会第111回大会, 2009年, 日本語, 国内会議黒毛和種繁殖雌牛の近年の泌乳量
口頭発表（一般）
- FOODEX JAPAN 2009, 2009年, 英語, 国際会議Secret of Wagyu
[招待有り]
シンポジウム・ワークショップパネル（指名）
- 日本畜産学会第104回大会講演要旨 p.80, 2005年, 日本語, 日本畜産学会, 東京大学, 国内会議黒毛和種における外貌記載法審査と繁殖形質との遺伝的関連性
口頭発表（一般）
- 日本畜産学会第104回大会講演要旨 p.83, 2005年, 日本語, 日本畜産学会, 東京大学, 国内会議血縁関係を考慮した黒毛和種種雄牛の最適な供用頻度の導出
口頭発表（一般）
- 第103回日本畜産学会, 2004年, 日本語, 日本畜産学会, 東京, 国内会議毛和種における外貌形質と繁殖形質との遺伝的関連性
口頭発表（一般）
- 第42回肉用牛研究会, 2004年, 日本語, 肉用牛研究会, 東京, 国内会議兵庫系黒毛和種（但馬牛）集団のジーンドロッピング法による系統分類の試み
口頭発表（一般）
- 第103回日本畜産学会大会, 2004年, 日本語, 日本畜産学会, 東京農工大学, 国内会議黒毛和種における外貌形質と繁殖形質との遺伝的関連性
口頭発表（一般）
- 未記入, 2004年, 英語, 未記入, 未記入, 国際会議Selective genotyping for marker assisted selection in Japanese Black cattle.
口頭発表（一般）
- 日本動物遺伝育種学会大会第4回大会, 2003年11月, 日本語, 日本動物遺伝育種学会, 明治大学生田校舎, 国内会議黒毛和種繁殖雌牛集団におけるセレクティブ・ジェノタイピング
口頭発表（一般）
- 日本動物遺伝育種学会大会第4回大会, 2003年11月, 日本語, 日本動物遺伝育種学会, 明治大学生田校舎, 国内会議ウシ体脂肪の脂肪酸組成に影響する遺伝的要因
口頭発表（一般）
- 第101回日本畜産学会大会, 2003年03月, 日本語, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, 国内会議黒毛和種繁殖雌牛の登録審査時に記録される外貌諸形質間の遺伝的関連性
口頭発表（一般）
- 第101回日本畜産学会大会, 2003年03月, 日本語, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, 国内会議黒毛和種繁殖雌牛の登録審査時に記載される外貌諸形質と枝肉形質間の遺伝的関連性
口頭発表（一般）
- 第101回日本畜産学会大会, 2003年03月, 日本語, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, 国内会議黒毛和種における種雄牛の供用パターンが集団構造に与える影響
口頭発表（一般）
- 第102回日本畜産学会, 2003年, 日本語, 日本畜産学会, 岐阜大学, 国内会議種雄牛の供用方法が但馬牛の集団構造に与える影響
口頭発表（一般）
- 第101回日本畜産学会大会講演要旨, 2003年, 日本語, 日本畜産学会, つくば, 国内会議種雄牛の供用方法が但馬牛の集団構造に与える影響
口頭発表（一般）
所属学協会
共同研究・競争的資金等の研究課題
- 学術研究助成基金助成金／基盤研究(C), 2018年04月 - 2021年03月
競争的資金
- 学術研究助成基金助成金／基盤研究(C), 2017年04月 - 2020年03月, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2016年04月 - 2020年03月
競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2015年04月 - 2018年03月
競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業 チーム型研究（CREST）, 2017年, 研究代表者放牧牛のインタラクション情報と生体・健康情報との関連性に関する分析と評価
競争的資金
- 公益社団法人畜産技術協会, 畜産・酪農生産力強化対策事業（繁殖性等向上対策）, 2017年, 研究代表者肉用子牛の体測定値推定技術を活用した母性能力向上のための研究開発・実証とその成果の普及
競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2014年04月 - 2016年03月
競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（CREST）, 2016年, 研究代表者放牧牛のインタラクション情報と生体・健康情報との関連性に関する分析と評価
競争的資金
- 科学研究費補助金／基盤研究(C), 2012年04月 - 2015年03月, 研究代表者
競争的資金
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