研究者紹介システム

乾 秀之
イヌイ ヒデユキ
バイオシグナル総合研究センター
准教授
農学関係
Last Updated :2021/04/12

研究者情報

所属

  • 【主配置】

    バイオシグナル総合研究センター
  • 【配置】

    農学部 生命機能科学科, 大学院農学研究科 生命機能科学専攻

学位

  • 博士(農学), 神戸大学

研究シーズ

授業科目

ミニ講義

ジャンル

  • 科学・技術 / 生命科学
  • 防災・くらし / 食生活

コメントテーマ

  • 環境モニタリング
  • 環境負荷軽減
  • PCBの毒性低下
  • 環境浄化
  • ファイトレメディエーション

研究ニュース

研究活動

研究分野

  • 環境・農学 / 植物保護科学

受賞

  • 2019年11月 日本農薬学会農薬環境科学研究会, 第37回農薬環境科学研究会, 汚染物質輸送因子の遺伝子発現と結合活性制御によるウリ科作物における作物汚染の低減化

    藤田健太郎、近藤恭光、本田香織、羽賀雄紀、長田裕之、松村千里、乾秀之

  • 2019年03月 日本農薬学会, 学生優秀発表賞, 汚染物質輸送因子の結合活性制御に着目したウリ科作物の汚染低減化

    藤田健太郎、近藤恭光、本田香織、羽賀雄紀、長田裕之、松村千里、乾秀之

  • 2017年08月 The 14th International Symposium on Persistent Toxic Substances, Student Poster Award, ossible metabolic fates of polychlorinated biphenyls by soil bacterium under complex pollution and its structural basis

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

  • 2017年03月 村尾育英会, 第34回村尾育英会学術賞, ウリ科植物のユニークな汚染物質蓄積機構

    乾秀之

  • 2016年10月 The 9th International PCB Workshop, Outstanding Student Research Award, Metabolic fates of CB118 by cytochrome P450 monooxygenase from soil bacterium under complex pollution and its structural basis

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

  • 2016年10月 The 9th International PCB Workshop, Outstanding Student Research Award, Congener-specific uptake of polychlorinated biphenyls by transporting factors in Cucurbita pepo

    Erika Isono, Hideyuki Inui

  • 2016年07月 The 13th International Symposium on Cytochrome P450 Biodiversity & Biotechnology, Honorable mention in the student presentation competition, Structural basis of CB118 metabolism by cytochrome P450 monooxygenase from Bacillus megaterium with decoy molecules

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

  • 2016年06月 環境化学会, 第25回環境化学討論会優秀学生賞, デコイ分子を用いたBacillus megaterium由来シトクロムP450モノオキシゲナーゼによるCB118の代謝とその構造基盤

    後藤絵里香、羽賀雄紀、久保惇、伊藤俊将、笠井千枝、荘司長三、山本恵子、松村千里、中野武、乾秀之

  • 2015年09月 植物の栄養研究会, 第1回植物の栄養研究会ポスター賞, ウリ科植物による脂溶性汚染物質の取り込み・蓄積メカニズム

    岩渕彩、乾秀之

  • 2014年11月 International Conference of Asian Environmental Chemistry 2014, Outstanding Student Research Award, Structural basis of metabolism of 2,3',4,4',5-pentachlorobiphenyl (CB118) by mammalian cytochrome P450 monooxygenases

    Shintarou Mise, Yuuki Haga, Miku Yabu, Toshimasa Itoh, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hideyuki Inui

  • 2014年11月 International Conference of Asian Environmental Chemistry 2014, Outstanding Student Research Award, Function and dynamic of transporting factors for persistent organic pollutants in Cucurbita pepo

    Junya Goto, Aya Iwabuchi, Hideyuki Inui

  • 2013年07月 環境化学会, 第22回環境化学討論会優秀学生賞, ラットおよびヒト由来シトクロムP450モノオキシゲナーゼによるCB118の代謝と3次元構造の比較

    見世慎太朗、羽賀雄紀、薮未来、伊藤俊将、山本恵子、鶴川正寛、松村千里、中野武、榊利之、乾秀之

  • 2011年07月 環境化学会, 第20回環境化学討論会優秀学生賞, ットおよびヒトCYP1A1によるポリ塩化ビフェニルの代謝と3次元構造の比較

    薮未来、山﨑清志、鈴木元治、羽賀雄紀、伊藤俊将、山本恵子、鶴川正寛、松村千里、中野武、榊利之、乾秀之

  • 2011年03月 ナス科・ウリ科ゲノム合同国際シンポジウム, ナス科・ウリ科ゲノム合同国際シンポジウム優秀ポスター発表賞, Promotion of tuberization by overexpression of Arabidopsis thaliana LOV KELCH REPEAT PROTEIN 2 in potato (Solanum tuberosum cv. May Queen)

    Yuuki Nishiyama 、Hideyuki Inui 、Yasunobu Ogura 、Takayoshi Shimazaki 、Akira Kikuchi 、Tomoyuki Takase、Tomohiro Kiyosue

  • 2011年02月 日本農芸化学会関西支部, 日本農芸化学会関西支部第468回講演会若手優秀発表賞, ウリ科植物における脂溶性汚染物質の体内移行に係わる因子

    沢田真美、松尾申遼、山﨑清志、乾秀之

  • 2009年10月 積水化学, 「積水化学 自然に学ぶものづくり」研究助成プログラム奨励賞, ウリ科植物の高脂溶性化合物蓄積メカニズムに学ぶ脂溶性化合物可溶化物質の開発

    乾秀之

  • 2009年06月 第18回環境化学討論会, ナイスプレゼンテーション賞, ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収経路の解明

    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, 朴 杓允, 今石 浩正, 乾 秀之

    国内学会・会議・シンポジウム等の賞

  • 2009年06月 環境化学会, 第18回環境化学討論会ナイスプレゼンテーション賞, ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収経路の解明

    山崎清志、前田耕作、細木直樹、玄康洙、池田健一、朴杓允、今石浩正、乾秀之

  • 2007年03月 日本生態学会, 第54回日本生態学会大会ポスター賞, 水生植物ヒルムシロ属における高温ストレス順応性の比較

    天野百々江、飯田聡子、岩崎哲史、深見泰夫、乾秀之、小菅桂子

  • 2006年03月 日本生態学会, 第53回日本生態学会大会ポスター賞, マイクロアレイによる水生植物ヒルムシロ属の高温ストレス応答の解析

    天野百々江、飯田聡子、岩崎哲史、深見泰夫、乾秀之、小菅桂子

  • 2001年03月 日本農薬学会, 平成13年度日本農薬学会 奨励賞, 薬物代謝酵素導入植物の作出とその残留農薬低減効果

    乾秀之

論文

  • Pesticide treatment reduces hydrophobic pollutant contamination in Cucurbita pepo through competitive binding to major latex-like proteins

    Fujita, K, Kondoh, Y, Honda, K, Haga, Y, Osada, H, Matsumura, C, Inui, H

    2020年, Environmental Pollution, 266, 115179, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Suppression of the genes responsible for transporting hydrophobic pollutants leads to the production of safer crops

    Fujita, K, Haga, Y, Yoshihara, R, Matsumura, C, Inui, H

    2020年, Science of the Total Environment, 741, 140439, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity

    Medic, A, Ljesevic, M, Inui, H, Beškoski, V, Kojic, I, Stojanovic, K, Karadzic, I

    2020年, RSC Advances, 10, 14060 - 14070, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Effects of Arabidopsis Ku80 deletion on the integration of the left border of T-DNA into plant chromosomal DNA via Agrobacterium tumefaciens

    Yoshihara, R., Mitomi, Y., Okada, M., Shibata, H., Tanokami, M., Nakajima, Y., Inui, H., Oono, Y., Furudate, H., Tanaka, S.

    2020年, Genes and Genetic Systems, 95, 1 - 10, 英語

    [査読有り]

    研究論文(学術雑誌)

  • High temperatures promote the uptake of hydrophobic pollutants by Cucurbita pepo via altered gene expression levels of major latex-like proteins

    Inui, H., Katte, N., Goto, J., Iwabuchi, A.

    2020年, Journal of Pesticide Science, 45 (2), 75 - 80, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Factors regulating the differential uptake of persistent organic pollutants in cucurbits and non-cucurbits

    Iwabuchi, A., Katte, N., Suwa, M., Goto, J., Inui, H.

    2020年, Journal of Plant Physiology, 245, 153094, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Uptake mechanisms of polychlorinated biphenyls in Cucurbita pepo via xylem sap containing major latex-like proteins

    後藤 純弥, 岩渕 彩, 吉原 亮平, 兒玉 典子, 松井 智美, 廣田 又士, 殷 熙洙, 乾 秀之

    2019年, Environmental and Experimental Botany, 162, 399 - 405, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Vladimir P. Beškoski, Atsushi Yamamoto, Takeshi Nakano, Katsuya Yamamoto, Chisato Matsumura, Mamoru Motegi, Latinka Slavković Beškoski, Hideyuki Inui

    We investigated the capability of microorganisms isolated from environments polluted with perfluoroalkyl acids (PFAAs) to conduct biotransformation of these emerging pollutants. Two different microbial consortia (chemoorganoheterotrophic bacteria and total yeast and molds) were isolated from two river sediments in Saitama and Osaka, Japan, known for long term pollution with perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA). The microbial consortia were incubated in the presence of added PFOS and PFOA, and decreases in concentrations of these compounds were between 46–69% and 16–36%, respectively. Decreases in concentrations were, in part, due to sorption on biomass, but defluorinated PFOS and PFOA products were not detected. However, untargeted analysis suggested the presence of several metabolites found only in samples from consortia with PFOS and PFOA but not in the control samples. Molecular formula candidates were narrowed down to two options, C18H28O5F and C21H27O4. It was assumed that these formulas were associated with unsaturated monofluorinated fatty acids and hydrocarbons with multiple unsaturated bonds or ring structures.

    Elsevier B.V., 2018年09月15日, Science of the Total Environment, 636, 355 - 359, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiichi Fukushi, Yuki Fujita, Junpei Nonogaki, Jun-ichi Tsujimoto, Takanari Hattori, Hideyuki Inui, Vladimir P. Beškoski, Hiroki Hotta, Mitsuru Hayashi, Takeshi Nakano

    We developed capillary zone electrophoresis (CZE) with indirect UV detection for the determination of fluoride (F−) in seawater using transient isotachophoresis (tITP) as an on-line concentration procedure. A method of correcting sample salinity effects was also proposed so that F− concentrations were obtained using a calibration graph. The proposed method is simple: it requires no sample pretreatment aside from dilution. The following optimum conditions were established: background electrolyte (BGE), 5 mM 2,6-pyridinedicarboxylic acid (PDC) adjusted to pH 3.5 containing 0.03% m/v hydroxypropyl methylcellulose (HPMC) detection wavelength, 200 nm vacuum (50 kPa) injection period of sample, 5 s (254 nL) and applied voltage, 23 kV with the sample inlet side as the cathode. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) for F− reached 0.024 and 0.070 mg/L, respectively. The respective values of the relative standard deviation (RSD) of the peak area, peak height, and migration time for F− were 2.5, 3.4, and 0.30%. The proposed method was applied for the determination of F− in seawater samples collected from coastal waters of western Japan during August 26–28, 2014. Both results obtained using standard addition method and a calibration graph agreed with those obtained using a conventional spectrophotometric method.

    Springer Verlag, 2018年02月01日, Analytical and Bioanalytical Chemistry, 410 (6), 1825 - 1831, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Metabolic enhancement of 2,3’,4,4’,5-pentachlorobiphenyl (CB118) using cytochrome P450 monooxygenase isolated from soil bacterium under the presence of perfluorocarboxylic acids (PFCAs) and the structural basis of its metabolism

    Goto, E, Haga, Y, Kubo, M, Itoh, T, Kasai, C, Shoji, O, Yamamoto, K, Matsumura, C, Nakano, T, 乾 秀之

    2018年, Chemosphere, 210, 376 - 383, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Veronika Navratilova, Marketa Paloncyova, Karel Berka, Shintaro Mise, Yuki Haga, Chisato Matsumura, Toshiyuki Sakaki, Hideyuki Inui, Michal Otyepka

    Background: Cytochromes P450 are major drug-metabolizing enzymes involved in the biotransformation of diverse xenobiotics and endogenous chemicals. Persistent organic pollutants (POPs) are toxic hydrophobic compounds that cause serious environmental problems because of their poor degradability. This calls for rational design of enzymes capable of catalyzing their biotransformation. Cytochrome P450 1A1 isoforms catalyze the biotransformation of some POPs, and constitute good starting points for the design of biocatalysts with tailored substrate specificity. Methods: We rationalized the activities of wild type and mutant forms of rat cytochrome P450 1A1 towards 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) and 3,3',4,4'-tetrachlorobiphenyl (PCB77) using experiments and molecular dynamics simulations. Results: We showed that the enhanced activity of the CYP1A1 mutant towards TCDD was due to more efficient binding of the substrate in the active site even though the mutated site was over 2.5 nm away from the catalytic center. Moreover, this mutation reduced activity towards PCB77. General significance: Amino acids that affect substrate access channels can be viable targets for rational enzyme design even if they are located far from the catalytic site.

    ELSEVIER SCIENCE BV, 2017年11月, BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1861 (11), 2852 - 2860, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Vladimir P. Beskoski, Katsuya Yamamoto, Atsushi Yamamoto, Hideo Okamura, Mitsuru Hayashi, Takeshi Nakano, Chisato Matsumura, Keiichi Fukushi, Shinpei Wada, Hideyuki Inui

    Perfluoroalkyl acids (PFAAs) including perfluoroalkyl sulfonates (PFSAs) and perfluoroallcyl carboxylates (PFCAs) were analyzed in sediment samples taken from Ajifu Waterway in Osaka city, from Osaka Bay, and from Kagoshima Bay, as well as in fifteen seawater samples collected from Osaka Bay and coastal waters of Western Japan. In all sediment samples, only PFCAs were detected, and the highest concentration was determined in Ajifu Waterway, where Sigma PFAA was 58990 ng kg(-1) dry weight. The total concentrations of PFAAs in sea water samples ranged between the limit of quantification and 53.4 ng L-1, and perfluorohexanoic acid was the most prevalent and had the highest concentration of 37 ng L-1. The changes in the patterns and concentrations of PFAA5 in Osaka Bay and coastal waters of Western Japan indicate that the PFAAs in surface waters are influenced by sources from Keihanshin Metropolitan Area, mainly the Yodo River basin, and the dilution effect which naturally occurs during their transport to the Pacific Ocean. (C) 2016 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2017年03月, CHEMOSPHERE, 170, 260 - 265, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shintaro Mise, Yuki Haga, Toshimasa Itoh, Akira Kato, Itsuko Fukuda, Erika Goto, Keiko Yamamoto, Miku Yabu, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hideyuki Inui

    Polychlorinated biphenyls (PCBs) accumulate in mammals via the food chain because of their characteristics such as slow degradation and high hydrophobicity. One of the 209 PCB congeners, 2,3',4,4',5-pentachlorobiphenyl (CB118), is abundantly found in the environment and in mammals. Understanding the metabolic fate of CB118 can provide important information toward evaluating its toxicity. In vitro studies on the metabolism of CB118 by cytochrome P450 enzymes (P450 or CYP) revealed that human CYP2B6 and rat CYP2B1 primarily metabolized it to 3-hydroxy (OH)-CB118, whereas rat CYP1A1 metabolized CB118 to 4-hydroxy-2,3,3',4',5-pentachlorobiphenyl (4-OH-CB107). Docking models of CYP2Bs with CB118 revealed a short distance between the 3-position of CB118 and the heme iron caused by polarization of the substrate-binding cavity, and maintenance of this pose through interaction with the peripheral amino acids determines the activity and position of hydroxylation. 4-Hydroxylation by rat CYP1A1 occurs owing to the longitudinal shape of the substrate-binding cavity toward the heme of CYP1A1. The metabolites 3-OH-CB118 and 4-OH-CB107 decreased potential for activating the aryl hydrocarbon receptor compared with that of CB118, thereby leading to a decrease in dioxin-like toxicity; however, the neurodevelopmental toxicity of 4-OH-CB107 has been previously reported. The results suggest that these 3 P450 isoforms play an important role in determining the extent of CB118 toxicity. This study will contribute to understanding of the metabolic fates and toxicity of CB118 in vivo.

    OXFORD UNIV PRESS, 2016年08月, Toxicological Sciences, 152 (2), 340 - 348, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideo Ohkawa, Hideyuki Inui

    A yeast gene expression system originally established for mammalian cytochrome P450 monooxygenase cDNAs was applied to functional analysis of a number of mammalian and plant P450 species, including 11 human P450 species (CYP1A1, CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1 and CYP3A4). The human P450 species CYP1A1, CYP1A2, CYP2B6, CYP2C18 and CYP2C19 were identified as P450 species metabolising various agrochemicals and environmental chemicals. CYP2C9 and CYP2E1 specifically metabolised sulfonylurea herbicides and halogenated hydrocarbons respectively. Plant P450 species metabolising phenylurea and sulfonylurea herbicides were also identified mainly as the CYP71 family, although CYP76B1, CYP81B1 and CYP81B2 metabolised phenylurea herbicides. The transgenic plants expressing these mammalian and plant P450 species were applied to herbicide tolerance as well as phytoremediation of agrochemical and environmental chemical residues. The combined use of CYP1A1, CYP2B6 and CYP2C19 belonging to two families and three subfamilies covered a wide variety of herbicide tolerance and phytoremediation of these residues. The use of 2,4-D-and bromoxynil-induced CYP71AH11 in tobacco seemed to enhance herbicide tolerance and selectivity. (c) 2014 Society of Chemical Industry

    WILEY-BLACKWELL, 2015年06月, PEST MANAGEMENT SCIENCE, 71 (6), 824 - 828, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Matashi Hirota, Junya Goto, Ryouhei Yoshihara, Noriko Kodama, Tomomi Matsui, Kiyoshi Yamazaki, Heesoo Eun

    Some cultivars of cucumbers, melons, pumpkins, and zucchini, which are members of the Cucurbitaceae family, are uniquely subject to contamination by hydrophobic pollutants such as the organohalogen insecticides DDT. However, the molecular mechanisms for the accumulation of these pollutants in cucurbits have not been determined. Here, cDNA subtraction analysis of Cucurbita pepo cultivars that are low and high accumulators of hydrophobic contaminants revealed that a gene for zinc finger proteins (ZFPs) are preferentially expressed in high accumulators. The cloned CpZFP genes were classified into 2 types: (1) the PEG type, which were expressed in C. pepo cultivars Patty Green, Black Beauty, and Gold Rush, and (2) the BG type, which were expressed in Black Beauty and Gold Rush. Expression of these CpZFP genes in transgenic tobacco plants carrying an aryl hydrocarbon receptor-based inducible gene expression system significantly induced E-glucuronidase activity when the plants were treated with a polychlorinated biphenyl (PCB) compound, indicating that highly hydrophobic PCBs accumulated in the plants. In transgenic tobacco plants carrying CpZFPs, accumulation of dioxins and dioxin-like compounds increased in their aerial parts when they were cultivated in the dioxin-contaminated soil. In summary, we propose that addition of CpZFP genes is a promising tool for conferring noncucurbits with the ability to accumulate hydrophobic contaminants. (C) 2014 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2015年03月, CHEMOSPHERE, 123, 48 - 54, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kiyoshi Yamazaki, Hiroki Tsuruta, Hideyuki Inui

    From previous reports, uptake and accumulation of organic compounds by plant roots are strongly related to water solubility. However, the relation between uptake pathways and water solubility remains unclear. Here, we used confocal laser scanning microscopy to observe the uptake of fluorescent hydrophilic and hydrophobic compounds in the living roots of Cucurbita pepo. We found strong regulation of the uptake of berberine, a hydrophobic compound at the endodermal Casparian strip, as previously reported, and similar regulation was also observed in the pericycle. Berberine was loaded into and transported upward through the protoxylem. Perylene, a highly hydrophobic compound, in contrast, passed through the Casparian strip and accumulated preferentially in the endodermis and pericycle. The results of our solvent extraction suggested that perylene diffused into the non-aqueous phase. Therefore, the uptake pathways for the hydrophilic and hydrophobic compoundss are different. These results offer a new way to understand the uptake of agrochemicals and pollutants and to select host plants for phytoremediation. (c) Pesticide Science Society of Japan

    PESTICIDE SCI SOC JAPAN, 2015年, JOURNAL OF PESTICIDE SCIENCE, 40 (3-4), 99 - 105, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Toshimasa Itoh, Keiko Yamamoto, Shin-Ichi Ikushiro, Toshiyuki Sakaki

    Polychlorinated dibenzo-p-dioxins (PCDDs) and coplanar polychlorinated biphenyls (PCBs) contribute to dioxin toxicity in humans and wildlife after bioaccumulation through the food chain from the environment. The authors examined human and rat cytochrome P450 (CYP)-dependent metabolism of PCDDs and PCBs. A number of human CYP isoforms belonging to the CYP1 and CYP2 families showed remarkable activities toward low-chlorinated PCDDs. In particular, human CYP1A1, CYP1A2, and CYP1B1 showed high activities toward monoCDDs, diCDDs, and triCDDs but no detectable activity toward 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-tetraCDD). Large amino acids located at putative substrate-recognition sites and the F-G loop in rat CYP1A1 contributed to the successful metabolism of 2,3,7,8-tetraCDD. Rat, but not human, CYP1A1 metabolized 3,3',4,4',5-pentachlorobiphenyl (CB126) to two hydroxylated metabolites. These metabolites are probably less toxic than is CB126, due to their higher solubility. Homology models of human and rat CYP1A1s and CB126 docking studies indicated that two amino acid differences in the CB126-binding cavity were important for CB126 metabolism. In this review, the importance of CYPs in the metabolism of dioxins and PCBs in mammals and the species-based differences between humans and rats are described. In addition, the authors reveal the molecular mechanism behind the binding modes of dioxins and PCBs in the heme pocket of CYPs.

    MDPI AG, 2014年08月, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 15 (8), 14044 - 14057, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Gion, Hideyuki Inui, Kazuyuki Takakuma, Takashi Yamada, Yumiko Kambara, Shuichi Nakai, Hiroyuki Fujiwara, Takashi Miyamura, Hiromasa Imaishi, Hideo Ohkawa

    Tobacco cytochrome P450 (CYP) 71AH11 metabolized the herbicide chlorotoluron, and its mRNA level was increased in tobacco culture cells by the treatment of 2,4-D. In order to clarify molecular mechanisms of induced gene expression of CYP71AH11 by herbicide treatment, a 1574-bp 5'-flanking region of CYP71AH11 was cloned, ligated to the reporter beta-glucuronidase (GUS) gene, and then transformed into tobacco plants. The GUS activity in the transgenic tobacco plants was highly induced by bromoxynil treatment, followed by 2,4-D. Chlorotoluron was slightly increased the GUS activity. The bromoxynil-increased GUS activity was partially repressed by the antioxidants, suggesting that reactive oxygen species may be involved in activation of the 5'-flanking region of CYP71AH11 by bromoxynil treatment. Deletion and mutation assays showed that the region CD (-1281 to -770 bp from the start codon of CYP71AH11) was important, but not sufficient, for response to bromoxynil. Electrophoretic mobility shift assays and southwestern blotting revealed that the sequences AAAAAG, and GAACAAAC and GAAAATTC in the CD region were important for interaction to the nuclear proteins of <30 and approximate to 75 kDa, respectively. Particularly, interaction between AAAAAG and <30 kDa proteins was increased by bromoxynil treatment. These results gave a cue for understanding the bromoxynil-induced gene expression of CYP71AH11, which may contribute to herbicide tolerance and selectivity in crop plants. (C) 2013 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014年01月, PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 108, 49 - 57, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryouhei Yoshihara, Shinryo Matsuo, Toru Iwata, Hideyuki Inui

    The solubilization of hydrophobic organic pollutants from soil is important for the phytoremediation of contaminated soil. In this study, we used perylene as a model pollutant to evaluate the abilities of 11 organic acids to solubilize hydrophobic compounds from soil. Oxalic acid and citric acid showed high solubilization abilities, and when added to soil, they enhanced perylene uptake into Cucurbita pepo, likely owing to the chelating ability of these acids. (C) Pesticide Science Society of Japan

    PESTICIDE SCI SOC JAPAN, 2014年, JOURNAL OF PESTICIDE SCIENCE, 39 (3-4), 162 - 164, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Gion, Hideyuki Inui, Kazuyuki Takakuma, Takashi Yamada, Yumiko Kambara, Shuichi Nakai, Hiroyuki Fujiwara, Takashi Miyamura, Hiromasa Imaishi, Hideo Ohkawa

    Tobacco cytochrome P450 (CYP) 71AH11 metabolized the herbicide chlorotoluron, and its mRNA level was increased in tobacco culture cells by the treatment of 2,4-D. In order to clarify molecular mechanisms of induced gene expression of CYP71AH11 by herbicide treatment, a 1574-bp 5'-flanking region of CYP71AH11 was cloned, ligated to the reporter beta-glucuronidase (GUS) gene, and then transformed into tobacco plants. The GUS activity in the transgenic tobacco plants was highly induced by bromoxynil treatment, followed by 2,4-D. Chlorotoluron was slightly increased the GUS activity. The bromoxynil-increased GUS activity was partially repressed by the antioxidants, suggesting that reactive oxygen species may be involved in activation of the 5'-flanking region of CYP71AH11 by bromoxynil treatment. Deletion and mutation assays showed that the region CD (-1281 to -770 bp from the start codon of CYP71AH11) was important, but not sufficient, for response to bromoxynil. Electrophoretic mobility shift assays and southwestern blotting revealed that the sequences AAAAAG, and GAACAAAC and GAAAATTC in the CD region were important for interaction to the nuclear proteins of <30 and approximate to 75 kDa, respectively. Particularly, interaction between AAAAAG and <30 kDa proteins was increased by bromoxynil treatment. These results gave a cue for understanding the bromoxynil-induced gene expression of CYP71AH11, which may contribute to herbicide tolerance and selectivity in crop plants. (C) 2013 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014年01月, PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 108, 49 - 57, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Mami Sawada, Junya Goto, Kiyoshi Yamazaki, Noriko Kodama, Hiroki Tsuruta, Heesoo Eun

    This is the first report, to our knowledge, to reveal important factors by which members of the Cucurbitaceae family, such as cucumber (Cucumis sativus), watermelon (Citrullus lanatus), melon (Cucumis melo), pumpkin (Cucurbita pepo), squash (C. pepo), and zucchini (C. pepo), are selectively polluted with highly toxic hydrophobic contaminants, including organochlorine insecticides and dioxins. Xylem sap of C. pepo ssp. pepo, which is a high accumulator of hydrophobic compounds, solubilized the hydrophobic compound pyrene into the aqueous phase via some protein(s). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of xylem sap of two C. pepo subspecies revealed that the amount of 17-kD proteins in C. pepo ssp. pepo was larger than that in C. pepo ssp. ovifera, a low accumulator, suggesting that these proteins may be related to the translocation of hydrophobic compounds. The protein bands at 17 kD contained major latex-like proteins (MLPs), and the corresponding genes MLP-PG1, MLP-GR1, and MLP-GR3 were cloned from the C. pepo cultivars Patty Green and Gold Rush. Expression of the MLP-GR3 gene in C. pepo cultivars was positively correlated with the band intensity of 17-kD proteins and bioconcentration factors toward dioxins and dioxin-like compounds. Recombinant MLP-GR3 bound polychlorinated biphenyls immobilized on magnetic beads, whereas recombinant MLP-PG1 and MLP-GR1 did not. These results indicate that the high expression of MLP-GR3 in C. pepo ssp. pepo plants and the existence of MLP-GR3 in their xylem sap are related to the efficient translocation of hydrophobic contaminants. These findings should be useful for decreasing the contamination of fruit of the Cucurbitaceae family as well as the phytoremediation of hydrophobic contaminants.

    AMER SOC PLANT BIOLOGISTS, 2013年04月, PLANT PHYSIOLOGY, 161 (4), 2128 - 2135, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Tetsuya Takeuchi, Akari Uesugi, Fumito Doi, Mikio Takai, Kosuke Nishi, Shiro Miyake, Hideo Ohkawa

    Coplanar polychlorinated biphenyls (Co-PCBs) consisting of non-ortho and mono-ortho-chlorinated PCBs are dioxin-like compounds and cause wide contamination in the environment. To monitor Co-PCB residues, it was attempted to establish an enzyme-linked immunosorbent assay (ELISA) with monoclonal and recombinant antibodies selective to Co-PCBs. When 3,3',5,5'-tetrachlorobiphenoxybutyric acid (PCBH)-keyhole limpet hemocyanin conjugate was immunized into mice, two monoclonal antibodies, Mab-0217 and Mab-4444, were obtained. 3,3'5,5'-Tetrachlorobiphenyl (PCB80) was determined with an IC50 value of 2.6 and 0.46 ng mL(-1) in ELISA based on Mab-0217 and Mab-4444, respectively. Mab-4444 cross-reacted with Co-PCB congeners, except for PCB77 and PCB81. Mab-0217 reacted with PCB80 and cross-reacted with PCB111. A single-chain variable fragment (scFv) antibody derived from Mab-4444 was produced in recombinant Escherichia coli cells. The scFv antibody showed nearly the same sensitivity toward PCBH as the parent monoclonal antibody in ELISA. These results clearly suggested that Mab-4444 and its scFv antibodies were suitable for monitoring the representative congeners of Co-PCBs.

    AMER CHEMICAL SOC, 2012年02月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60 (7), 1605 - 1612, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Gion, Hideyuki Inui, Hideaki Sasaki, Yasushi Utani, Susumu Kodama, Hideo Ohkawa

    Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of beta-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g(-1) PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.

    TAYLOR & FRANCIS INC, 2012年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 47 (7), 599 - 607, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Keiko Gion, Yasushi Utani, Taketo Wakai, Susumu Kodama, Heesoo Eun, Yun-Seok Kim, Hideo Ohkawa

    The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated beta-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorinated dibenzeno-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of residential and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g(-1) of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.

    TAYLOR & FRANCIS INC, 2012年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 47 (1), 59 - 65, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Keiko Gion, Yasushi Utani, Taketo Wakai, Susumu Kodama, Heesoo Eun, Yun-Seok Kim, Hideo Ohkawa

    The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated beta-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorodibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of life and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g(-1) of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.

    TAYLOR & FRANCIS INC, 2012年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 47 (4), 233 - 239, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Gion, Hideyuki Inui, Hideaki Sasaki, Yasushi Utani, Susumu Kodama, Hideo Ohkawa

    Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of beta-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g(-1) PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.

    TAYLOR & FRANCIS INC, 2012年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 47 (7), 599 - 607, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yoshiki Nagaba, Muhammad Tufail, Hideyuki Inui, Makio Takeda

    The giant water bug, Lethocerus deyrollei has become an endangered species in Japan. For conservation of this insect, hormonal regulation and the potential involvement of environmental pollutants as endocrine disrupting chemicals (EDCs) in reproduction was investigated using vitellogenin (Vg), the major yolk protein precursor, gene as a biomarker. For this, the cDNA encoding Vg of L. deyrollei was cloned and the amino acid sequence was deduced. The Vg polypeptide sequence was deduced from the 5,865 bp cDNA, containing several well-conserved features of insect Vgs. SDS-PAGE showed two Vg subunit polypeptides of about 193 and 56 kDa in L. deyrollei. Northern blot analysis revealed that the Vg gene was transcribed specifically in the female fat body and Vg expression was induced when females in reproductive diapause were injected with juvenile hormone (JH) III and inhibited when they were injected with 20-hydroxyecdysone (20E), suggesting that the Vg gene is regulated by the JH in L. deyrollei. Dramatic induction or inhibition of Vg gene transcript was detected when diapausing females were injected with EDCs. 17 beta-estradiol and bisphenol A enhanced the expression but 4-t-octylphenol and 4-nonylphenol repressed it. These results indicate that EDCs may disrupt the reproductive physiology of L. deyrollei in nature, providing a first indication that environmental pollutants could be the cause of the rapid disappearance of this species from Japanese ecosystems.

    SPRINGER, 2011年06月, JOURNAL OF INSECT CONSERVATION, 15 (3), 421 - 431, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sayuri Shimazu, Hideyuki Inui, Hideo Ohkawa

    Molecular mechanisms of metabolism and modes of actions of agrochemicals and related compounds are important for understanding selective toxicity, biodegradability, and monitoring of biological effects on nontarget organisms. It is well-known that in mammals, cytochrome P450 (P450 or CYP) monooxygenases metabolize lipophilic foreign compounds. These P450 species are inducible, and both CYP1A1 and CYP1A2 are induced by aryl hydrocarbon receptor (AhR) combined with a ligand. Gene engineering of P450 and NADPH cytochrome P450 oxidoreductase (P450 reductase) was established for bioconversion. Also, gene modification of AhRs was developed for recombinant AhR-mediated beta-glucronidase (GUS) reporter assay of AhR ligands. Recombinant P450 genes were transformed into plants for phytoremediation, and recombinant AhR-mediated GUS reporter gene expression systems were each transformed into plants for phytomonitoring. Transgenic rice plants carrying CYP2B6 metabolized the herbicide metolachlor and remarkably reduced the residues in the plants and soils under paddy field conditions. Transgenic Arabidopsis plants carrying recombinant guinea pig (g) AhR-mediated GUS reporter genes detected PCB126 at the level of 10 ng/g soils in the presence of biosurfactants MEL-B. Both phytomonitoring and phytoremediation plants were each evaluated from the standpoint of practical uses.

    AMER CHEMICAL SOC, 2011年04月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59 (7), 2870 - 2875, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Taketo Wakai, Keiko Gion, Kiyoshi Yamazaki, Yun-Seok Kim, Heesoo Eun

    Zucchini cultivars Cucurbita pepo subsp. ovifera cv. Patty Green and subsp. pepo CV. Gold Rush were cultivated hydroponically in a nutrient solution supplemented with a mixture of dioxins and dioxin-like compounds. Patty Green and Gold Rush showed low and high accumulation of these compounds in the aerial parts respectively. In both cultivars, the accumulation of each congener negatively depended on its hydrophobicity. This suggests that desorption and solubilization were partly responsible for congener specificity of accumulation, since this was not found in soil experiments. In contrast, no clear difference in accumulation in the roots was observed between the cultivars, whereas the translocation factors, which are indicators of efficient translocation from the roots to the aerial parts, differed among the congeners hydrophobicity-dependently. There were positive correlations between accumulation in the roots and the hydrophobicity of the polychlorinated biphenyl congeners in both cultivars. These results indicate that translocation was also partly responsible for the congener specificity and accumulation concentrations.

    TAYLOR & FRANCIS LTD, 2011年04月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75 (4), 705 - 710, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinryo Matsuo, Kiyoshi Yamazaki, Keiko Gion, Heesoo Eun, Hideyuki Inui

    Nine zucchini cultivars (Cucurbita pepo subspecies ovifera and pepo) were grown in soil containing a mixture of dioxins and dioxin-like compounds. Plants of subspecies pepo accumulated polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and polychlorinated biphenyls (PCBs) in their aerial parts at higher concentrations than did plants of subspecies ovifera. In all cultivars, the accumulation of PCDDs and PCDFs in the aerial parts decreased with increasing hydrophobicity, whereas for PCBs, a negative correlation was observed in only two cultivars. The other seven cultivars selectively accumulated ortho-chlorinated biphenyls. Our results contribute to the understanding of the mechanisms underlying these phenomena, which should help in the development of efficient methods for phytoremediation of hydrophobic contaminants. (C) Pesticide Science Society of Japan

    PESTICIDE SCI SOC JAPAN, 2011年, JOURNAL OF PESTICIDE SCIENCE, 36 (3), 363 - 369, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kiyoshi Yamazaki, Motoharu Suzuki, Toshimasa Itoh, Keiko Yamamoto, Miki Kanemitsu, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Yasuo Fukami, Hiromasa Imaishi, Hideyuki Inui

    Coplanar polychlorinated biphenyls included in dioxin-like compounds are bio-accumulated and adversely affect wildlife and human health. Although many researchers have studied the metabolism of PCBs, there have been few reports of the in vitro metabolism of 3,3',4,4',5-pentachlorobiphenyl (PCB126), despite the fact that it has the highest toxicity among PCB congeners. Cytochrome P450 (CYP) 1A1 proteins can metabolize some dioxins and PCBs by hydroxylation, but the activities of human and rat CYP1A1 proteins are very different. The mechanism remains unclear. From our results, rat CYP1A1 metabolized PCB126 into 4-OH-3,3',4',5-tetrachlorobiphenyl and 4-OH-3,3',4',5,5'-pentachlorobiphenyl, but human CYP1A1 did not metabolize. Homology models of the two CYP proteins, and docking studies, showed that differences in the amino acid residues forming their substrate-binding cavities led to differences in the size and shape of the cavities; only the cavity of rat CYP1A1 allowed PCB126 close enough to the haem to be metabolized. Comparison of the amino acid residues of other mammalian CYP1A1 proteins suggested that rats have a unique metabolism of xenobiotics. Our results suggest that it is necessary to be careful in human extrapolation of toxicity data estimated by using the rat as an experimental animal, especially in the case of compounds metabolized by CYP1A1.

    OXFORD UNIV PRESS, 2011年, JOURNAL OF BIOCHEMISTRY, 149 (4), 487 - 494, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Yasunobu Ogura, Tomohiro Kiyosue

    Potato tuberization is induced under short-day conditions and repressed under long-day conditions. In this study, we produced transgenic potatoes overexpressing either Arabidopsis thaliana LOV KELCH PROTEIN 2 (35S:LKP2) or CONSTANS fused with a transcription repressor motif (35S: CO-Rep). In an in vitro tuberization assay, the average number of tubers per plant was greater in 35S: LKP2 plants than in vector-control plants, but lower in 35S: CO-Rep plants. Under long-day conditions in soil, all 35S: LKP2 plants tuberized, whereas most control plants and 35S: CO-Rep plants did not. These results suggest genes involved in flowering time regulation can be used to control potato tuber production. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

    ELSEVIER SCIENCE BV, 2010年06月, FEBS LETTERS, 584 (11), 2393 - 2396, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 抗体可変領域を用いた抗体転写因子の高感度化に影響を与えるドメイン配列

    河野実織, 櫻井由季, 祗園景子, 乾秀之

    2010年, 生化学, ROMBUNNO.1P - 1287, 日本語

    研究論文(その他学術会議資料等)

  • Sayuri Shimazu, Yukiko Kawabata, Akito Inayoshi, Hideyuki Inui, Hitoshi Ashida, Hideo Ohkawa

    Four expression plasmids for recombinant human aryl hydrocarbon receptor (hAhR) consisting of a ligand binding domain of hAhR, a DNA-binding domain of LexA and a transactivation domain of VP16 as well as beta-glucuronidase (GUS) reporter genes were constructed. All the expression plasmids were transformed into tobacco plants. The selected transgenic tobacco plants were used to assay. PCB congeners showed GUS activity in a TEF-dependent manner. The selected transgenic tobacco plant XhD4V17 was compared with the transgenic tobacco plants XmD4V26 and XgD2V23 containing recombinant mouse (m) AhR-mediated GUS reporter gene expression cassette and recombinant guinea pig (g) AhR-mediated GUS reporter gene expression cassette for PCB congener-inducible GUS activity. The data revealed that the tobacco plant XgD2V23 was the most active in PCB congener-inducible GUS activity. In a 1: 1 mixture of PCB126 and PCB80 a reduced PCB126-induced GUS activity was observed in plant XgD2V23, which could possibly be due to interaction between PCB126 and PCB80.

    TAYLOR & FRANCIS INC, 2010年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 45 (8), 741 - 749, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sayuri Shimazu, Yukiko Kawabata, Akito Inayoshi, Hideyuki Inui, Hitoshi Ashida, Hideo Ohkawa

    Four expression plasmids for recombinant human aryl hydrocarbon receptor (hAhR) consisting of a ligand binding domain of hAhR, a DNA-binding domain of LexA and a transactivation domain of VP16 as well as beta-glucuronidase (GUS) reporter genes were constructed. All the expression plasmids were transformed into tobacco plants. The selected transgenic tobacco plants were used to assay. PCB congeners showed GUS activity in a TEF-dependent manner. The selected transgenic tobacco plant XhD4V17 was compared with the transgenic tobacco plants XmD4V26 and XgD2V23 containing recombinant mouse (m) AhR-mediated GUS reporter gene expression cassette and recombinant guinea pig (g) AhR-mediated GUS reporter gene expression cassette for PCB congener-inducible GUS activity. The data revealed that the tobacco plant XgD2V23 was the most active in PCB congener-inducible GUS activity. In a 1: 1 mixture of PCB126 and PCB80 a reduced PCB126-induced GUS activity was observed in plant XgD2V23, which could possibly be due to interaction between PCB126 and PCB80.

    TAYLOR & FRANCIS INC, 2010年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 45 (8), 741 - 749, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hormonal regulation and effects of fur environmental pollutants on vitellogenin gene transcription in the giant water bug; Lethocerus deyrollei (Hemiptera; Belostomatidae).

    Yoshiki Nagaba, Tufail M, Hideyuki Inui, Makio Takeda

    2010年, J. Insect Conservation, 15, 421 - 431, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sayuri Shimazu, Masaya Ohta, Hideyuki Inui, Yoshihiko Nanasato, Hitoshi Ashida, Hideo Ohkawa

    The transgenic Arabidopsis plants carrying a recombinant guinea pig (g) aryl hydrocarbon receptor (AhR)-mediated beta-glucuronidase (GUS) reporter gene expression system were generated for assays of polychlorinated biphenyl (PCB) congeners. The selected transgenic Arabidopsis plant XgD2V11-6 exhibited a correlation between uptake of PCB126 and PCB126-induced GUS activity. Also, the plants showed induced GUS activity towards the supplemental indole 3-acetic acid (IAA). Thus, the GUS assay may reflect induction by both endogenous and exogenous AhR ligands. When biosurfactants, MEL-B, produced in the culture of yeast isolated from plants were used for assays of PCB congeners in the transgenic Arabidopsis plants, they showed marked PCB126 dose-dependent and toxic equivalency factor (TEF) dependent GUS activities. The effects of biosurfactants were clearer when the plants were cultivated on soils containing PCB congeners for 7 days as compared with on soils for 3 days as well as in the medium for 3 days. Threfore, it was estimated that biosurfactants form micellae with PCB congeners, which are easily uptaken by the plants in a mode of passive diffusion, transport into the aerial parts and then induce GUS activity.

    TAYLOR & FRANCIS INC, 2010年, JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 45 (8), 750 - 756, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Biosurfactants useful for assays of PCB congeners in transgenic arabidopsis plants carrying a recombinant guinea pig AhR-mediated GUS reporter gene expression system

    SHIMAZU Sayuri, OHTA Masaya, INUI Hideyuki, NANASATO Yoshiko, ASHIDA Hitoshi, OHKAWA Hideo

    2010年, Journal of Environmental Science and Health B, 45, 773 - 779, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Hideaki Sasaki, Nam-Hai Chua, Hideo Ohkawa

    Transgenic Arabidopsis plants carrying a recombinant human estrogen receptor gene and a green fluorescent protein reporter gene were used to bioassay estrogenic compounds. We constructed four recombinant human estrogen receptor genes by combining the DNA-binding domain of LexA, a synthetic nuclear localization signal, a ligand-binding domain of the human estrogen receptor, and a transactivation domain of VP16 in different orders; the XEV plants were the most sensitive, and were able to detect 0.001 ng ml(-1) of 17-estradiol (E(2)). The transgenic plants absorbed E(2) and 4-nonylphenol present in the nutrient solution, whereas most of the other compounds seemed to be retained in, or on, the roots. Estrone, methoxychlor, bisphenol A, 4-nonylphenol, and 4-t-octylphenol in the medium were clearly detected by RT-PCR and PCR of the genomic DNA. The transgenic Arabidopsis XEV plants thus have potential for the bioassay of estrogenic compounds.

    SPRINGER, 2009年12月, TRANSGENIC RESEARCH, 18 (6), 899 - 909, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Gion, Yuki Sakurai, Akito Watari, Hideyuki Inui

    Many recombinant transcription factors have been invented, but we cannot select a substance used as an inducer. In this study, we have created a novel expression control system in which we can select a substance as an inducer toward which a monoclonal antibody (mAb) is prepared. The variable region fragments (Fvs) of the heavy and light chains (V(H) and V(L)) of the bisphenol A (BPA)-specific mAb BBA-2187 were each fused to the DNA-binding domain (DBD) of LexA and the transactivation domain (AD) of VP16. The association between the two recombinant proteins in the presence of BPA constituted a functional transcription factor. The recombinant proteins in which the DBD was fused to the N-terminal side of the Fv and in which the nuclear localization signal (NLS) was fused to the N-termini of the construct including the AD highly induced beta-galactosidase (lacZ) expression in recombinant yeast cells grown with BPA. When the Fvs of the polychlorinated biphenyl (PCB)-specific mAb 4444 were used, DBD-NLS-V(H) and NLS-AD-V(L) showed significantly increased lacZ activity in response to a PCB derivative. The Fv transcription factor may be useful in many fields such as gene therapeutics.

    AMER CHEMICAL SOC, 2009年12月, ANALYTICAL CHEMISTRY, 81 (24), 10162 - 10166, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keiko Gion, Yuki Sakurai, Akito Watari, Hideyuki Inui

    Many recombinant transcription factors have been invented, but we cannot select a substance used as an inducer. In this study, we have created a novel expression control system in which we can select a substance as an inducer toward which a monoclonal antibody (mAb) is prepared. The variable region fragments (Fvs) of the heavy and light chains (V(H) and V(L)) of the bisphenol A (BPA)-specific mAb BBA-2187 were each fused to the DNA-binding domain (DBD) of LexA and the transactivation domain (AD) of VP16. The association between the two recombinant proteins in the presence of BPA constituted a functional transcription factor. The recombinant proteins in which the DBD was fused to the N-terminal side of the Fv and in which the nuclear localization signal (NLS) was fused to the N-termini of the construct including the AD highly induced beta-galactosidase (lacZ) expression in recombinant yeast cells grown with BPA. When the Fvs of the polychlorinated biphenyl (PCB)-specific mAb 4444 were used, DBD-NLS-V(H) and NLS-AD-V(L) showed significantly increased lacZ activity in response to a PCB derivative. The Fv transcription factor may be useful in many fields such as gene therapeutics.

    AMER CHEMICAL SOC, 2009年12月, ANALYTICAL CHEMISTRY, 81 (24), 10162 - 10166, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kosuke Nishi, Yasuhiro Goda, Shigeru Fujimoto, Hideyuki Inui, Hideo Ohkawa

    Alkylphenol polyethoxylates and alkylphenols are widely distributed contaminants in the environment. Two anti-alkylphenol polyethoxylate monoclonal antibodies MOF3-139 and AP-14 were established to measure these chemicals by enzyme immunoassays in previous studies. Interestingly, these two monoclonal antibodies showed different specificity; AP-14 cross-reacts with nonylphenoxyacetic acid and nonylphenol, whereas MOF3-139 does not. To understand the molecular basis of the difference in specificity, single-chain Fv (scFv) antibodies derived from the monoclonal antibodies were each produced in Escherichia coli cells and characterized in competitive enzyme-linked immunosorbent assay. The scFv antibodies exhibited comparable reactivity profiles to the derived parent monoclonal antibodies. It was found that the VH domain of AP-14 play an important role in the cross-reaction when specificity tests were performed using variable domain-swapped scFv antibodies. An experiment using complementarity-determining region (CDR)-grafted scFv antibodies revealed that CDRI and CDR2 of AP-14 are involved in the cross-reaction to nonylphenoxyacetic acid and nonylphenol, respectively. Site-directed mutagenesis was introduced in both regions and the assay revealed that 33rd Thr and 35th His in VH domain of AP-14 were highly involved in the cross-reaction with nonylphenoxyacetic acid and that 33rd Thr, 57th Asp, and 59th Glu were involved in the cross-reaction with nonylphenol. The findings herein would contribute to the antibody engineering for specificity modification and to the generation of an alkylphenol-specific recombinant antibody by antibody engineering. (C) 2009 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2009年09月, MOLECULAR IMMUNOLOGY, 46 (15), 3125 - 3130, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 抗体可変領域を用いた転写因子によるポリ塩化ビフェニル測定法の高感度化

    櫻井由季, 祗園景子, 乾秀之

    2009年09月, 生化学, ROMBUNNO.4T15P - 5, 日本語

    研究論文(その他学術会議資料等)

  • 抗体可変領域を用いた人工転写因子の交叉反応性

    櫻井由季, 祗園景子, 山崎清志, 乾秀之

    2009年09月, 生化学, ROMBUNNO.4T15P - 7, 日本語

    研究論文(その他学術会議資料等)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収・蓄積性能

    乾秀之, 山崎清志, 岩田徹, 松尾申遼, 沢田真美, 祗園景子, 若井丈人, KIM Yun‐Seok, EUN Heesoo

    2009年06月, 環境化学討論会講演要旨集, 18th, 420 - 421, 日本語

    研究論文(その他学術会議資料等)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収・移行・蓄積性能

    乾秀之, 若井丈人, 祗園景子, 金倫碩, 殷熈洙

    2009年03月, 日本農芸化学会大会講演要旨集, 2009, 306, 日本語

    研究論文(その他学術会議資料等)

  • Susumu Kodama, Kumiko Okada, Keiko Akimoto, Hideyuki Inui, Hideo Ohkawa

    Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, beta-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the beta-glucuronidase reporter gene. The results clearly suggest that XDVs are functional transcription factors that respond to AhR ligands, and that the XDV-mediated reporter gene expression system is applicable to bioassays for dioxin residues in the environment.

    WILEY-BLACKWELL PUBLISHING, INC, 2009年02月, PLANT BIOTECHNOLOGY JOURNAL, 7 (2), 119 - 128, 英語

    [査読有り]

    研究論文(学術雑誌)

  • ズッキーニにおけるダイオキシン類の品種特異的蓄積

    松尾申遼, 山崎清志, 祗園景子, EUN Heesoo, 乾秀之

    2009年02月, 日本農薬学会大会講演要旨集, 34th, 115, 日本語

    研究論文(その他学術会議資料等)

  • How are Recombinant P450s and AhRs useful for Phytomonitoring and Phytoremediation of Environmental Chemicals?

    Ohkawa Hideo, Shimazu Sayuri, Inui Hideyuki

    Contamination of the environment and agricultural products with persistent organic pollutants (POPs) including dioxins and organochlorine insecticides is a serious problem in the world. It is important to monitor these residues and also to reduce the residues in the environment. Microsomal cytochrome P450 (P450) species in mammals were screened for degradation of pesticide residues. The cDNA clones of the selected P450 species were express en in higher plants. Also, aryl hydrocarbon receptor (AhR)-mediated beta-glucuronidase (GUS) reporter gene expression systems were introduced into higher plants. The transgenic plants were evaluated for phytoremediation and for phytomonitoring. We discussed how recombinant P450s and AhRs were useful for both purposes.

    MEDIMOND S R L, 2009年, 16TH INTERNATIONAL CONFERENCE ON CYTOCHROME P450, PROCEEDINGS, 133 - +, 英語

    研究論文(国際会議プロシーディングス)

  • Inui, H, Takehara, A, Doi, F, Nishi, K, Takai, M, Miyake, S, Ohkawa, H

    This is a report on the development of immunoaffinity chromatography using a column of silica gel with an immobilized single-chain variable fragment (scFv) antibody specific to bisphenol A (BPA) for cleanup of BPA-contaminated water samples. The BBA-2187 scFv antibody specific to BPA was purified from the periplasmic fractions of the recombinant Escherichia coli. After a sample of BPA-contaminated river water was applied to the immunoaffinity column, the background signal intensity observed in high-performance liquid chromatography (HPLC) analysis of the eluates was markedly lower than that observed in HPLC analysis of the eluates from an Oasis HLB cartridge treated with the same sample. The immunoaffinity column efficiently concentrated BPA from actual river water samples with different matrices. Our results demonstrate that the immunoaffinity column with immobilized BBA-2187 scFv antibody is efficient for the cleanup of BPA-contaminated water samples from different sources.

    AMER CHEMICAL SOC, 2009年01月, Journal of Agricultural and Food Chemistry, 57 (2), 353 - 358, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A high-throughput bioassay for dioxins and dioxin-like compounds using transgenic Arabidopsis plants expressing a recombinant aryl hydrocarbon receptor gene

    INUI Hideyuki, Keiko GionGION Keiko, Heesoo Eun, Hideo Ohkawa

    2009年, The proceedings of the 16th International Conference on Cytochrome P450 Biochemistry, Biophysics, Biotechnology, 103 - 106, 英語

    研究論文(国際会議プロシーディングス)

  • A high-throughput bioassay for dioxins and dioxin-like compounds using transgenic Arabidopsis plants expressing a recombinant aryl hydrocarbon receptor gene

    H. Inui, K. Gion, H. Eun, H. Ohkawa

    We engineered tobacco and Arabidopsis plants, in which the expression of the reporter beta-glucuronidase (GUS) gene was induced by the recombinant aryl hydrocarbon receptor (AhR). These transgenic plants showed dose-dependent increase of GUS activities toward dioxin-like polychlorinated biphenyl (PCB) congeners. On the other hand, GUS activities did not increase when non-toxic PCBs were treated. From these results, we found that there was positive correlation between GUS activity and dioxin toxicity. The transgenic Arabidopsis plants grown in 1 g of soil contaminated with dioxin-like PCBs showed significant increase of GUS activity, leading to a high-throughput screening of dioxin contamination without any extraction and purification steps. This system has a potential fir the bioassay of dioxins and dioxin-like compounds.

    MEDIMOND S R L, 2009年, 16TH INTERNATIONAL CONFERENCE ON CYTOCHROME P450, PROCEEDINGS, 103 - +, 英語

    研究論文(国際会議プロシーディングス)

  • Hideyuki Inui, Taketo Wakai, Keiko Gion, Yun-Seok Kim, Heesoo Eun

    The zucchini (Cucurbita pepo) cultivars 'Patty Green', 'Black Beauty', and 'Gold Rush' were cultivated on weathered dioxin-contaminated soil in pots, and concentrations of the 29 dioxin-like compounds that were assigned WHO-TEFs, three non-toxic polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs), and two non-dioxin-like polychlorinated biphenyls (PCBs) were analyzed. Toxic equivalent (TEQ) values accumulated in 'Black Beauty' and 'Gold Rush' were about 180 times higher than those in 'Patty Green'. The bioconcentration factor (BCF) based on total mass concentration of the twelve dioxin-like PCBs was higher than those of the seven PCDDs and ten PCDFs in all the cultivars. The BCFs for PCDD and PCDF congeners were negatively correlated with octanol-water partition coefficients in all the plants. No correlations were observed in PCB congeners in the high accumulators, although in 'Patty Green' the BCFs for PCB congeners were significantly correlated with octanol-water partition coefficients. Our findings suggest that the high accumulators had unknown, unique mechanisms for uptake of PCBs, whereas PCDDs and PCDFs were absorbed based on their physicochemical properties. (C) 2008 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2008年11月, CHEMOSPHERE, 73 (10), 1602 - 1607, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Taketo Wakai, Keiko Gion, Yun-Seok Kim, Heesoo Eun

    The zucchini (Cucurbita pepo) cultivars 'Patty Green', 'Black Beauty', and 'Gold Rush' were cultivated on weathered dioxin-contaminated soil in pots, and concentrations of the 29 dioxin-like compounds that were assigned WHO-TEFs, three non-toxic polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs), and two non-dioxin-like polychlorinated biphenyls (PCBs) were analyzed. Toxic equivalent (TEQ) values accumulated in 'Black Beauty' and 'Gold Rush' were about 180 times higher than those in 'Patty Green'. The bioconcentration factor (BCF) based on total mass concentration of the twelve dioxin-like PCBs was higher than those of the seven PCDDs and ten PCDFs in all the cultivars. The BCFs for PCDD and PCDF congeners were negatively correlated with octanol-water partition coefficients in all the plants. No correlations were observed in PCB congeners in the high accumulators, although in 'Patty Green' the BCFs for PCB congeners were significantly correlated with octanol-water partition coefficients. Our findings suggest that the high accumulators had unknown, unique mechanisms for uptake of PCBs, whereas PCDDs and PCDFs were absorbed based on their physicochemical properties. (C) 2008 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2008年11月, CHEMOSPHERE, 73 (10), 1602 - 1607, 英語

    [査読有り]

    研究論文(学術雑誌)

  • アリルハイドロカーボン受容体を導入した形質転換植物によるダイオキシン類のバイオアッセイ

    祗園景子, 大川秀郎, 乾秀之

    2008年06月, 環境化学討論会講演要旨集, 17th, 268 - 269, 日本語

    [招待有り]

    研究論文(その他学術会議資料等)

  • 抗体可変領域を用いた新規遺伝子発現調節システム

    祗園景子, 渡昭人, 櫻井由季, 乾秀之

    2008年, 生化学, 1P - 1361, 日本語

    研究論文(その他学術会議資料等)

  • Hideyuki Inui, Atsuyo Maeda, Hideo Ohkawa

    Microsomal cytochrome P450 3A4 (CYP3A4) catalyzes monooxygenase reactions toward a diverse group of exogenous and endogenous substrates and requires cytochrome b(5) (b(5)) in the oxidation of the typical substrate testosterone. To analyze the molecular interaction among CYP3A4, NADPH-cytochrome P450 oxidoreductase (P450 reductase), and b(5), we constructed several fused enzyme genes and expressed them in Saccharomyces cerevisiae. The recombinant fused enzymes CYP3A4-truncated (t)-P450 reductase-t-b(5) (3RB) and CYP3A4-t-b(5)-t-P450 reductase (3BR) in yeast microsomes showed a higher specific activity in 6 beta-hydroxylation of testosterone than did the reconstitution premixes of CYP3A4, P450 reductase, and b(5). The purified fused enzymes exhibited lower K-m values and substantially increased V-max values in 6 beta-hydroxylation of testosterone and oxidation of nifedipine. Moreover, the fused enzymes showed significantly higher activities in cytochrome c reduction than the reconstitution premixes. Although the affinity of 3RB toward cytochrome c was twice as high as that of 3BR, 3BR and 3RB showed nearly the same affinity toward NADPH/NADH. In addition, the heme of the CYP3A4 moiety of 3RB was reduced preferentially and more rapidly than that of 3BR, whereas the heme of the b(5) moiety of 3BR was selectively reduced compared with that of 3RB. These results suggest that the conformation of the 3RB molecule was the most suitable for high activity because of appropriate ordering of the CYP3A4, P450 reductase, and b(5) moieties for efficient electron flow. Thus, we believe that the b(5) moiety plays an important role in the efficient transfer of the second electron in the vicinity of the CYP3A4 moiety.

    AMER CHEMICAL SOC, 2007年09月, BIOCHEMISTRY, 46 (35), 10213 - 10221, 英語

    [査読有り]

    研究論文(学術雑誌)

  • KODAMA Susumu, OKADA Kumiko, INUI Hideyuki, OHKAWA Hideo

    2007年, Planta, in press, 英語

    [査読有り]

    研究論文(学術雑誌)

  • S Hirose, H Kawahigashi, K Ozawa, N Shiota, H Inui, H Ohkawa, Y Ohkawa

    The human gene for CYP2B6, a cytochrome P450 monooxygenase that inactivates xenobiotic chemicals, was introduced into Oryza sativa cv. Nipponbare by Agrobacterium-mediated transformation. At germination, R, seeds of transgenic rice plants expressing CYP2136 (CYP2136 rice) showed a high tolerance to 5 mu M metolachlor, a preemergence herbicide that is degraded by CYP2136. Thin-layer chromatography after culture with C-14-Iabeled metolachlor revealed that the amounts of residual metolachlor decreased in plant tissues and the medium of CYP2136 rice faster than those of untransformed Nipponbare. CYP2136 rice plants were able to grow in the presence of 13 out of 17 herbicides: five chloroacetamides and mefenacet, pyributicarb, amiprofos-methyl, trifluralin, pendimethalin, norflurazon, and chlorotoluron. These herbicides differ in their modes of action and chemical structures. Transgenic rice expressing a xenobiotic-degrading human CYP2136, which has broad substrate specificity, should be good not only for developing herbicide tolerant rice but also for reducing the environmental impact of agrochernicals.

    AMER CHEMICAL SOC, 2005年05月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 53 (9), 3461 - 3467, 英語

    [査読有り]

    研究論文(学術雑誌)

  • H Kawahigashi, S Hirose, H Inui, H Ohkawa, Y Ohkawa

    We introduced an expression plasmid, pIKBACH, co-expressing the human cytochrome P450 genes CYP1A1, CYP2B6, and CYP209, into rice plants (Oryza sativa cv. 'Nipponbare'). The transgenic rice plants (pIKBACH rice) were screened by a combination of hygromycin resistance, PCR, and Western blot analysis. The pIKBACH rice plants expressed all three P450 species and exhibited tolerance towards various herbicides with different chemical structures and different modes of action, including a photosynthesis inhibitor (chlortoluron), very long-chain fatty acids (VLCFAs) inhibitors (acetochlor, metolachlor, and thenylchlor), a carotenoid biosynthesis inhibitor (norflurazon), and a root-elongation inhibitor (pyributicarb). In addition, the pIKBACH rice plants showed high tolerance to two mixtures of three herbicides [quizalofop-ethyl (0.15 muM), metolachlor (2 muM), and norflurazon (0.4 muM); and mefenacet (2.5 muM), thenylchlor (2 muM), and pyributicarb (1.5 muM)]. Thin-layer chromatography analysis revealed that the pIKBACH transgenic rice plants exhibited high metabolic activity towards chlortoluron, metolachlor, and norflurazon. The metabolism of herbicides by the pIKBACH rice plants was enhanced additively by the introduced P450 species. Assuming that public and commercial acceptance is forthcoming, pIKBACH rice plants may become useful tools for the breeding of herbicide-tolerant crops and for phytoremediation of environmental pollution by organic chemicals. (C) 2004 Elsevier Ireland Ltd. All fights reserved.

    ELSEVIER IRELAND LTD, 2005年03月, PLANT SCIENCE, 168 (3), 773 - 781, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Monitoring of endocrine disruptors in transgenic plants carrying aryl hydrocarbon receptor and estrogen receptor genes

    H Inui, H Sasaki, S Kodama, NH Chua, H Ohkawa

    Transgenic plants carrying mammalian hormone receptor genes were examined for monitoring of environmental chemicals including dioxins and endocrine disruptors. The transgenic tobacco plants expressing mouse arylhydrocarbon receptor gene detected 1ppb of the dioxin-like compound 20-methylcholanthrene in the cultured plants. In addition, the transgenic Arabidopsis plants expressing human estrogen receptor gene detected 1ppt of 17 beta-estradiol and ppb levels of the other endocrine disruptors such as bisphenol A, 4-t-octylphenol, and others in potted plants. These transgenic plants appear to be useful for on site monitoring of dioxins and estrogenic contaminants in the environment. The transgenic plants expressing AhR and ER detected 1ppb of MC under cultured conditions and 1ppt of E-2 under potted conditions, respectively. Basic technology was established for monitoring dioxins and EDs in the transgenic plants with the receptor genes. However, increase of the sensitivity towards other EDs with low affinity towards receptors is necessary. In order to overcome this problem, (1)sensitive receptors may be useful instead of mouse AhR and human ER. It is known that fish ERs were more sensitive to alkylphenols such as nonylphenol and octylphenol than mammalian ones. (2)Increase of uptake efficiency of dioxins and EDs is another way to increase sensitivity. Zucchini plants are known to have efficient translocation of polychlorinated dibenzo-p-dioxins and dibenzofurans(7). Furthermore, (3)genes synthesizing flower colors as reporter genes may be useful for visible monitoring of contaminations on site.

    AMER CHEMICAL SOC, 2005年, NEW DISCOVERIES IN AGROCHEMICALS, 892, 40 - 47, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sakiko Hirose, Hiroyuki Kawahigashi, Tomomi Inoue, Hideyuki Inui, Hideo Ohkawa, Yasunobu Ohkawa

    We compared three transgenic rice plants transformed with pIJ2C9, pIES2C9, or pIJU2C9, which express the human CYP2C9 gene under the control of the CaMV 35S, chimeric CaMV 35S, or maize polyubiquitin 1 promoter, respectively. The plants, especially those transformed with pIJU2C9, showed high tolerance to the sulfonylurea herbicides chlorsulfuron, imazosulfuron, and triasulfuron, owing to metabolic detoxification promoted by the introduced CYP2C9. The levels of expression of CYP2C9 were highly related to the tolerance to sulfonylurea herbicides. In this study, we achieved to produce transgenic rice plants with high expression of CYP2C9 by use of a maize polyubiquitin 1 promoter. Imazosulfuron is used in rice, but chlorsulfuron and triasulfuron are not, because of the sensitivity of rice plants to them. Transgenic rice plants expressing CYP2C9 will be useful for introducing tolerance to chlorsulfuron and triasulfuron into rice lines. Copyright © 2005 The Japanese Society for Plant Cell and Molecular Biology.

    Japanese Society for Plant Cell and Molecular Biology, 2005年, Plant Biotechnology, 22 (2), 89 - 96, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Ryu Yamada, Tetsuya Yamada, Yasunobu Ohkawa, Hideo Ohkawa

    We have developed a selection system for transformation of Arabidopsis thaliana via Agrobacterium tumefaciens. This selection system uses human cytochrome P450 monooxygenases that metabolize herbicides. Herbicide-tolerant seedlings transformed with CYP1A1, CYP2B6, CYP2C9, or CYP2C19 were selected with the herbicides acetochlor, amiprophos-methyl, chlorpropham, chlorsulfuron, norflurazon, and pendimethalin. The herbicide-tolerant plants transformed with CYP1A1, CYP2B6, and CYP2C19 expressed the corresponding P450 cDNAs. Inheritance and segregation of the P450 genes were analyzed in T2 progeny of herbicide-tolerant T1 seedlings. Metabolism of [14C] norflurazon by transgenic T2 seedlings expressing CYP1A1 produced non-toxic N-demethylated norflurazon. This result suggests that the P450 species expressed in transgenic Arabidopsis plants coordinately functioned as selectable markers because of active metabolism of the herbicides. Copyright © 2005 The Japanese Society for Plant Cell and Molecular Biology.

    Japanese Society for Plant Cell and Molecular Biology, 2005年, Plant Biotechnology, 22 (4), 281 - 286, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T Yamada, T Ishige, N Shiota, H Inui, H Ohkawa, Y Ohkawa

    A rat P450 monooxygenase gene (CYP1A1) was introduced into potato plants to enhance the metabolism of the environmental contaminants in subterranean organs. The CYP1A1 gene was kept under the control of the potato patatin promoter to enhance tuber-specific expression. A total of 106 transgenic plants (PAT1A1 plants) were obtained following selection by a resistance test to kanamycin and PCR analysis. PAT1A1 plants treated with 10% exogenous sucrose showed a higher activity of monooxgenase in the leaves than the non-transgenic plants. This indicated that the activity enhanced by 10% sucrose was due to the patatin promoter containing the sucrose-inducted elements. One representative transgenic plant, Ag2197, was selected on the basis of monooxgenase activity in the leaves and Western blot analysis. Ag2197 was found to accumulate a large amount of CYP1A1 mRNA and protein in the developing tuber but not in the mature tuber. The residual herbicides, atrazine and chlortoluron, were analyzed in the micro-tubers of Ag2197 and non-transgenic plants. The amount of residual herbicides in Ag2197 was much lower than that in the non-transgenic plant, indicating that the transgenic plant metabolized the herbicides to a detoxified form. The transgenic plants produced in this study might be useful for the phytoremediation of chemical pollution in the soil.

    SPRINGER-VERLAG, 2002年09月, THEORETICAL AND APPLIED GENETICS, 105 (4), 515 - 520, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Inducible cross-tolerance to herbicides in transgenic potato plants with the rat CYP1A1 gene

    T Yamada, Y Ohashi, M Ohshima, H Inui, N Shiota, H Ohkawa, Y Ohkawa

    A gene of the enzyme involved in xenobiotic metabolism in mammalian liver was introduced into potato to confer inducible herbicide tolerance. A rat cytochrome P450 monooxygenase, CYP1A1 cDNA, was kept under the control of the tobacco PR1a promoter in order to apply the system of chemical inducible expression using the plant activator Benzothiadiazole (BTH). Transgenic plants were obtained based on the kanamycin resistance test and PCR analysis. Northern-blot analysis revealed the accumulation of mRNA corresponding to rat CYP1A1 in the transgenic plants treated with BTH (3.0 mumol/pot), whereas no accumulation of the corresponding mRNA occurred without BTH treatment. These transgenic plants also produced a protein corresponding to CYP1A1 in the leaves by BTH treatment. The transgenic plants with BTH application showed a much-higher tolerance to the phenylurea herbicides chlortoluron and methabenzthiazuron than non-transgenic plants. These findings indicated that the ability of metabolizing the two herbicides to less-toxic derivatives was displayed in the transgenic plants after BTH treatment. Transgenic plants harboring the CYP1A1 cDNA fused with the yeast P450 reductase (YR) gene under the control of PR1a were also produced. Although the plants showed a lower expression level of the fused gene than transgenic plants with CYP1A1 cDNA alone, they were tolerant to herbicides. These facts suggested that the CYP1A1 enzyme fused with YR showed a higher specific activity than CYP1A1 alone. This study demonstrated that the mammalian cDNA for the detoxification enzyme of herbicides under the control of the PR1a promoter conferred chemical-inducible herbicide tolerance on potato.

    SPRINGER-VERLAG, 2002年02月, THEORETICAL AND APPLIED GENETICS, 104 (2-3), 308 - 314, 英語

    [査読有り]

    研究論文(学術雑誌)

  • H Inui, N Shiota, Y Ido, T Inoue, S Hirose, H Kawahigashi, Y Ohkawa, H Ohkawa

    The cDNAs of human drug-metabolizing cytochrome P450 species CYP209 and CYP2C19 were each expressed in rice (Oryza sativa L.) plants under the control of CaMV35S promoter and Nos terminator. Western blot analysis of the selected transgenic rice plants revealed that CYP2C9 and CYP209 were produced in the transgenic rice plants 2C9-57 and 2C19-12, respectively, [(14)C]Chlorsulfuron and [(14)C]imazosulfuron were found to be more rapidly metabolized in R(2) seedlings front 2C9-57R(1) than in the control plants to yield polar metabolites which were mainly glucose conjugates of hydroxylated metabolites formed by the function of CYP2C9. The R, transgenic rice seeds from 2CO-57R, showed tolerance toward the sulfonylurea herbicide chlorsulfuron in a germination test, On the other hand. I(14)[C]pyributicarb was also rapidly metabolized in R(1) seedlings from 2C19-12R(0) to give polar metabolites. but not in the control and PCR-negative transgenic plants. The R, transgenic rice seeds of 2C19-12R(0) showed cross-tolerance toward the herbicides mefenacet, metolachlor, norflurazon, and pyributicarb with different chemical structures and modes of herbicide action in a germination test. It was Suggested that the herbicide-tolerant rice plant 2C957R(2) seems to be practically useful for evasion of the sensitivity of rice plants toward the sulforlylurea herbicide chlorsulfuron. In addition, the herbicide-tolerant 2C19-12R(1) appears to be potentially useful for the control of suffonylurea-resistant weeds in a paddy field by the combined use of the herbicides mefenacet, metolachlor, norflurazon, and pyributicarb. (C) 2001 Elsevier Science.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2001年11月, PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 71 (3), 156 - 169, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Metabolism of herbicides and other chemicals in human cytochrome P450 species and in transgenic potato plants co-expressing human CYP1A1, CYP2B6 and CYP2C19

    H Inui, N Shiota, Y Motoi, Y Ido, T Inoue, T Kodama, Y Ohkawa, H Ohkawa

    Metabolism of 50 herbicides and 7 other chemicals with different structures and modes of action was examined in the microsomes from the recombinant yeast strains expressing each of ii human P450 species. Of these, 27 herbicides and 6 other chemicals were mainly metabolized by CYP1A1, CYP1A2, CYP2B6, CYP2C9, CYP2C18, CYP2C19, CYP2D6 and CYP3A4. The metabolites of atrazine (AT), chlortoluron (CT), fenitrothion (FT), methoxychlor (MXC), pyributicarb (PC), simazine (SZ) and simetryn (ST) were identified by co-chromatography with the authentic standards as well as LC/MS and GC/MS of their derivatives. Based on these results, three major P450 species CYP1A1, CYP2B6 and CYP2C19 in different families and subfamilies were selected and then co-expressed in the transgenic potato plant T1977, which showed cross-tolerance toward the herbicides acetochlor (AC), AT, CT, methabenzthiazuron (MT), metolachlor (MC), norflurazon (NR) and PC.(1)) The T1977 plant showed a higher activity in the metabolism of each of [C-14] CT, [C-14] AT, [C-14] PC and [C-14] MXC than the control. In T1977, [C-14] PC was almost completely metabolized to m-tert-butylphenol(BP), O-demethylated PC (DMPC), hydrox ylated BP (BPOH) and unknown metabolites. Also, [C-14] MXC was rapidly metabolized in T1977 through O-demethylation to yield mono-demethylated and di-demethyrated metabolites, which were mainly deposited as glucosides in the plants. These results strongly suggested that three P450 species expressed in T1977 coordinately functioned and actively metabolized the herbicides and the insecticide. The transgenic plants expressed human P450 species metabolizing xenobiotics seem to be useful for generation of transgenic crops with low pesticide residues as well as for phytoremediation of pesticide residues.

    PESTICIDE SCI SOC JAPAN, 2001年, JOURNAL OF PESTICIDE SCIENCE, 26 (1), 28 - 40, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Expression of human cytochromes P450 1A1 and P450 1A2 as fused enzymes with yeast NADPH-cytochrome P450 oxidoreductase in transgenic tobacco plants

    N Shiota, S Kodama, H Inui, H Ohkawa

    Among 11 isoforms of the human cytochrome P450 enzymes metabolizing xenobiotics, CYP 1A1 and CYP 1A2 were major P450 species in the metabolism of the herbicides chlortoluron and atrazine in a yeast expression system. CYP1A2 was more active in the metabolism of both herbicides than CYP1A1, The fused enzymes of CYP1A1 and CYP1A2 with yeast NADPH-cytochrome P450 oxidoreductase were functionally active in the microsomal fraction of the yeast Saccharomyces cerevisiae and showed increased specific activity towards 7-ethoxyresorufin as compared to CYP1A1 and CYP1A2 alone. Then, both fused enzymes were each expressed in the microsomes of tobacco (Nicotiana tabacum cv, Samsun NN) plants. The transgenic plants expressing the CYP1A2 fusion enzyme had higher resistance to the herbicide chlortoluron than the plants expressing the CYP1A1 fusion enzyme did, The transgenic plants expressing the CYP1A2 fused enzyme metabolized chlortoluron to a larger extent to its non-phytotoxic metabolites through N-demethylation and ring-methyl hydroxylation as compared to the plants expressing the CYP1A1 fused enzyme. Thus, the possibility of increasing the herbicide resistance in the transgenic plants by the selection of P450 species and the fusion with P450 reductase is discussed.

    TAYLOR & FRANCIS LTD, 2000年10月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64 (10), 2025 - 2033, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Herbicide metabolism and cross-tolerance in transgenic potato plants co-expressing human CYP1A1, CYP2B6, and CYP2C19

    H Inui, T Kodama, Y Ohkawa, H Ohkawa

    The transgenic potato plants T1977, co-expressing human CYP1A1, CYP2B6, and CYP2C19; S1965, expressing human CYP1A1; S1972, expressing human CYP1B6; and S1974, expressing human CYP2C19 were selected by the combination of kanamycin-resistance, PCR, 7-ethoxycoumarin O-deethylase assay, and Western blot analysis. The 7-ethoxyresorufin O-deethylase activity of both T1977 and S1965 expressing CYP1A1 were 27 and 53 times higher, respectively, than that of the control. T1977 exhibited remarkable cross-tolerance toward the photosynthesis-inhibiting herbicides atrazine (AT), chlortuluron (CT), and methabenzthiazuron (MT), the lipid biosynthesis-inhibiting herbicides acetochlor (AC) and metolachlor (MC), and the carotenoid biosynthesis-inhibiting herbicide norflurazon (NR), although the control did not show tolerance toward these herbicides. In addition, T1977 showed high tolerance toward pyributicarb (PC), which inhibits root elongation, whereas S1974 expressing CYP2C19 showed a weak resistance to the herbicide, which was found to be metabolized by CYP2C19 in vitro. S1965, S1972, and S1974 showed cross-tolerance toward AT, CT, MT, and NR; AC and MC; and AT, AC, and MC; respectively. These results suggested that T1977 co-expressing CYP1A1, CYP2B6, and CYP2C19 was highly cross-tolerant reward all the herbicides tested, probably by cooperative herbicide metabolism of three P450 species, which were found to metabolize these herbicides in vitro. The transgenic plants expressing human P450 species metabolizing xenobiotics seem to be useful for breeding of herbicide-tolerant crops as well as fur phyroremediation of environmental contaminants. (C) 2000 Academic Press.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2000年02月, PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 66 (2), 116 - 129, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Herbicide metabolism and cross-tolerance in transgenic potato plants expressing human CYP1A1

    H Inui, Y Ueyama, N Shiota, Y Okhawa, H Ohkawa

    Transgenic potato plants expressing human CYP1A1 and human CYP1Al/yeast NADPH-cytochrome P450 reductase (YR) fused enzyme were generated from microtubers by the use of an Agrobacterium transformation system. The transgenic plants S1384 expressing human CYP1A1 and both F1386 and F1515 expressing the fused enzyme were selected by kanamycin resistance, PCR analysis, chlortoluron (CT) resistance, and Western blot analysis. The integration and transcription of the corresponding CYP1A1 genes were confirmed in these selected transgenic plants by Southern and Northern blot analyses. CYP1A1 and its fused proteins were found to be produced in the transgenic plants S1384 and F1515, respectively. The P450-dependent monooxygenase activity of the transgenic plants S1384, S1386, and F1515 was 3.5, 4.2, and 3.8 times higher in 7-ethoxycoumarin O-deethylation in vitro and 6.4, 5.8, and 5.3 times higher in [(14)C]CT metabolism in viva than those of the control plants, respectively. In the metabolism of [(14)C]atrazine (AT), four metabolites were found in both control and transgenic plants. The deisopropylated deethylated metabolite DIDE, which is nonphytotoxic, was produced to a higher extent in S1384 and F1515 compared with the control. With herbicide tolerance tests, S1384 showed tolerance toward both AT and pyriminobacmethyl (PM), and F1386 and F1515 were tolerant toward PM, while the control died by treatment with both herbicides. Thus, it was found that the transgenic potato plants expressing human CYP1A1 metabolized the herbicides CT and AT with different structures and herbicide modes of action and resulted in cross-tolerance to both herbicides as well as PM. (C) 1999 Academic Press.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 1999年05月, PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 64 (1), 33 - 46, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Herbicide metabolism and resistance of transgenic potato plants expressing rat cytochrome P4501A1

    H Inui, N Shiota, T Ishige, Y Ohkawa, H Ohkawa

    It was attempted to generate transgenic potato plants expressing rat P4501Al and rat P4501A1/yeast reductase (YR) fused enzyme by the use of Agrobacterium-transformation system, Six regenerated plants for P4501Al (GC) and twenty plants for P4501Al/YR fused enzyme (GFC) were selected by kanamycin resistance, Southern blot analysis revealed that one of GC plants and ten of GFC plants showed several bands of P450 cDNA or its fused enzyme gene. The GC plant No.1160 was found to produce a detectable amount of mRNA corresponding to 1.6 kb-P450 cDNA, whereas the GFC plants gave a much less amount of mRNA corresponding to 3.5 kb-P450/YR fused enzyme gene. Western blot analysis showed that the GC plant No.1160 produced a large amount of a 59kDa protein corresponding to rat P4501Al. No GFC plants gave a detectable amount of a 130 kDa fused protein corresponding to rat P4501Al/YR fused enzyme, 7-Ethoxycoumarin O-deethylase and cytochrome c oxidoreductase activities of the transgenic plants were 1.4 to 3.5 and 1.3 to 3.5 times higher than those of the control plants, respectively, [C-14]-Labeled chlortoluron(CT) was more rapidly metabolized through N-demethylation and p-methyl hydroxylation to none or less phytotoxic metabolites in the GC plant No,1160, while it was also metabolized through N-demethylation in the control plants. In vivo herbicide-tolerant tests revealed that the GC plant No,1160 was tolerant against the spray of the CT at 10 mu mol per pot and another herbicide DCMU, [3-(3,4-dichlorophenyl)-1,1-dimethylurea], at 2 mu mol per pot, although the control plant completely died under the same concentrations of both herbicides. Thus, it was found that expression of rat P4501Al cDNA confer tolerance to the phenylurea herbicides in potato plants.

    JAPANESE SOC BREEDING, 1998年06月, BREEDING SCIENCE, 48 (2), 135 - 143, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Inui, Noriaki Shiota, Teruo Ishige, Yasunobu Ohkawa, Hideo Ohkawa

    It was attempted to generate transgenic potato plants expressing rat P4501A1 and rat P4501A1/yeast reductase (YR) fused enzyme by the use of Agrobacterium-transformation system. Six regenerated plants for P4501A1 (GC) and twenty plants for P4501A1/YR fused enzyme (GFC) were selected by kanamycin resistance. Southern blot analysis revealed that one of GC plants and ten of GFC plants showed several bands of P450 cDNA or its fused enzyme gene. The GC plant No.1160 was found to produce a detectable amount of mRNA corresponding to 1.6 kb-P450 cDNA, whereas the GFC plants gave a much less amount of mRNA corresponding to 3.5 kb-P450/YR fused enzyme gene. Western blot analysis showed that the GC plant No. 1160 produced a large amount of a 59kDa protein corresponding to rat P4501AI. No GFC plants gave a detectable amount of a 130kDa fused protein corresponding to rat P4501A1/YR fused enzyme. 7-Ethoxycoumarin O-deethylase and cytochrome c oxidoreductase activities of the transgenic plants were 1.4 to 3.5 and 1.3 to 3.5 times higher than those of the control plants, respectively. [14C]-Labeled chlortoluron(CT) was more rapidly metabolized through N-demethylation and p-methyl hydroxylation to none or less phytotoxic metabolites in the GC plant No.1160, while it was also metabolized through N-demethylation in the control plants. In vivo herbicide-tolerant tests revealed that the GC plant No.1160 was tolerant against the spray of the CT at 10μmol per pot and another herbicide DCMU, [3-(3,4-dichlorophenyl) - 1,1 - dimethylurea], at 2μmol per pot, although the control plant completely died under the same concentrations of both herbicides. Thus, it was found that expression of rat P4501A1 cDNA confer tolerance to the phenylurea herbicides in potato plants.

    Japanese Society of Breeding, 1998年, Breeding Science, 48 (2), 135 - 143, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Metabolism of the herbicide chlortoluron in transgenic tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 and yeast NADPH-cytochrome P450 oxidoreductase

    N Shiota, H Inui, H Ohkawa

    Metabolism of the herbicide chlortoluron was examined in transgenic tobacco plants expressing the genetically engineered fused enzyme between rat cytochrome P4501A1 and yeast NADPH-cytochrome P450 oxidoreductase. The transgenic plants were resistant to chlortoluron at concentrations of 1D to 50 mu M in Murashige and Skoog medium; however, 10 mu M was toxic to control plants. There were no significant differences in the uptake and the translocation of [C-14] chlortoluron between transgenic and control plants. The major metabolites produced by transgenic plants were N-demethylated metabolite, 4-carboxyphenyl metabolite, and their conjugates, whereas only the N-demethylated metabolite was produced by control plants. In vitro studies also confirmed that the fused enzyme expressed in the microsomal fraction of the transgenic plants exhibited both ring-methyl hydroxylation and N-demethylation activities toward chlortoluron. These results suggested that the transgenic tobacco plants expressing the fused enzyme metabolized chlortoluron to yield larger amounts of nonphytotoxic metabolites, resulting in tolerance to the herbicide. (C) 1996 Academic Press, Inc.

    ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 1996年03月, PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 54 (3), 190 - 198, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • Selective uptake of polychlorinated biphenyls by Cucurbita pepo

    Inui, H, Yamazaki, K, Matsuo, S, Yoshihara, R, Eun, H

    2012年, Organohalogen Compounds, 74, 1213 - 1216, 英語

  • どんな物質でも誘導剤にできる抗体可変領域を用いた新規遺伝子発現調節システム

    乾秀之

    2012年, 生物化学的測定研究会年報, 16 pp.63-69, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • SEKISUI 自然に学ぶものづくり 第75回ウリ科植物に学ぶ土壌浄化法

    乾秀之

    2010年, Newton, 9月号, P4, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • バイオテクノロジーと実用技術 どんな物質でも誘導剤にできる抗体可変領域を用いた遺伝子発現調節システム

    乾秀之, 祗園景子, 櫻井由季, 渡昭人, 山崎清志

    2009年10月, ケミカルエンジニヤリング, 54 (10), 767 - 772, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 免疫化学測定の実際と課題-ISO技術検討課題提案の背景と例示-

    嶋津小百合, 乾秀之, 三宅司郎, 山科清, 大川秀郎

    2009年, 生物化学的測定研究会年報, 13, pp.61 - 72, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • どんな物質でも誘導剤にできる抗体可変領域を用いた遺伝子発現調節システム

    乾秀之, 祗園景子, 櫻井由季, 渡昭人, 山崎清志

    2009年, ケミカルエンジニヤリング, 4 (10), pp.35 - 40, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • Hideyuki Inui, Keiko Gion, Yasushi Utani, Hideo Ohkawa

    John Wiley and Sons, 2007年05月31日, Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety, 431 - 438, 英語

  • AGRO 137-Bioassays for persistent organic pollutants in receptor-mediated reporter gene expression systems

    Hideo Ohkawa, Hideyuki Inui, Yoshikazu Tanaka

    AMER CHEMICAL SOC, 2007年03月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 233, 397 - 397, 英語

    研究発表ペーパー・要旨(国際会議)

  • 11th IUPAC International Congress of Pesticide Chemistry、Biopesticides and Transgenic Crops

    乾秀之

    2007年, 日本農薬学会誌, 32(Supplement), pp.S18 - S21, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 11th IUPAC International Congress of Pesticide Chemistry、Biomonitoring of POPs in Transgenic Flowering Plants

    乾秀之

    2007年, 日本農薬学会誌, 32(Supplement), pp.S160 - S161, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 11th IUPAC International Congress of Pesticide Chemistry、Biochemical Assays for POPs

    乾秀之

    2007年, 日本農薬学会誌, 32(Supplement), pp.S163 - S164, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • Monitoring of nano-level concentrations of persistent organic pollutants (POPs) in transgenic plants

    Hideo Ohkawa, Hideyuki Inui, Yoshikazu Tanaka

    AMER CHEMICAL SOC, 2006年03月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 231, 英語

    研究発表ペーパー・要旨(国際会議)

  • 植物バイオテクノロジーの新たなる挑戦、「環境モニタリング・浄化植物」

    川畑 順子, 乾 秀之, 大川 秀郎

    2005年, BIOINDUSTRY, 22 8 25-32, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 教育・情報公開・環境と農薬科学「21世紀農薬科学への提言」

    乾 秀之

    2005年, 日本農薬学会誌, 30 3 307-308, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • Herbicide resistance in transgenic plants with mammalian P450 monooxygenase genes

    INUI Hideyuki, OHKAWA Hideo

    2005年, Pest Management Science, 61 286-291, 英語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • ヒトのシトクロムP450を利用した環境浄化サツマイモの開発

    田畑和文, 星野文彦, 堀英津子, 光川典宏, 平井正名, 川東広幸, 乾秀之, 大川秀郎

    2004年11月25日, 日本分子生物学会年会プログラム・講演要旨集, 27th, 1014, 日本語

  • チトクロームP450の分子機能を難分解性有機汚染物質のモニタリングや負荷軽減へ応用する

    児玉 進, 乾 秀之, 大川 秀郎

    2003年, 化学と生物, 41 7 464-470, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • PPCPS特集、TOPIC-A: New Discoveries:

    石原 亨, 乾 秀之

    2003年, 日本農薬学会誌, 28,502-516, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 薬物代謝酵素導入植物の作出とその残留農薬低減効果

    INUI Hideyuki

    2001年, 日本農薬学会誌, 26 318-326, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 3分子種のヒトP 4 5 0 を導入したイネの除草剤耐性

    川東 広幸, 広瀬 咲子, 乾 秀之, 大川 秀郎, 大川 安信

    2000年09月25日, 育種学研究 = Breeding research, 2 (2), 日本語

  • Survey of xenobiotic-metabolizing cytochrome P450 homologues in higher plant species

    Watanabe, N. K, Ohkawa, H, Takeda, Y, Inui, H, Kasai, K, Ohkawa, H

    2000年, Memoirs of the Research Institute of Biology-Oriented Science and Technology Kinki University, 5, 9 - 18, 英語

    記事・総説・解説・論説等(学術雑誌)

  • Hideo Ohkawa, Hiromasa Imaishi, Noriaki Shiota, Takashi Yamada, Hideyuki Inui, Yasunobu Ohkawa

    Japanese Society for Plant Cell and Molecular Biology, 1998年, Plant Biotechnology, 15 (4), 173 - 176, 英語

    記事・総説・解説・論説等(学術雑誌)

  • Molecular mechanisms of herbicide resistance

    H Ohkawa, H Imaishi, N Shiota, H Inui, Y Ohkawa

    Mechanisms of herbicide resistance include modified target site, enhanced detoxification, and alterations in the uptake, translocation, or compartmentalization of the chemical. Triazine resistance, one of the most prevalent types of herbicide resistance found in weeds, is due to a modification at the target site, the D1 protein of photosystem II: in chloroplasts. The phenylurea herbicide chlortoluron is metabolized in wheat and barley via ring-methyl hydroxylation and N-demethylation. Resistance of these crops to the herbicide has been attributed largely to ring-methyl hydroxylation probably catalized by cytochrome P450. When P4501A1/yeast reductase fused enzyme was placed in tobacco plants, the transgenic plants showed resistance to chlortoluron and metabolized it more rapidly than did control plants, mainly via ring-methyl hydroxylation as well as N-demethylation. When mt P4501A1 and its fused enzyme with yeast reductase were each expressed in potato plants, both transgenic plants showed resistance to the herbicides chlortoluron and DCMU. Since mammals contain a large number of P450 species with overlapping broad substrate-specificity towards xenobiotics, expression of each of these species in plants seems to produce transgenic plants not only resistant to herbicides but also enhanced in metabolism of various environmental contaminants.

    I O S PRESS, 1998年, PESTICIDES AND THE FUTURE, 2 245-252, 245 - 252, 英語

    記事・総説・解説・論説等(学術雑誌)

  • Cytochrome P450 monooxygenases metabolizing herbicides

    H Ohkawa, N Shiota, H Imaishi, T Yamada, H Inui, Y Ohkawa

    Cytochrome P450 monooxygenases catalyze oxidative reactions in biosynthesis of secondary metabolites as well as in metabolism of xenobiotics in higher plants. The enzyme system localized on the microsomes of plant cells consists of many cytochrome P450 (P450 or CYP) species and one or a few species of NADPH-cytochrome P450 reductase (P450 reductase). Particularly, P450 species metabolizing herbicides play an important role in r herbicide selectivity and resistance. However, molecular information on plant species metabolizing herbicides was quite limited. It was found that tobacco cultured S401 cells treated with 2,4-D metabolized chlortoluron to give ring-methyl hydroxylated and N-demethylated metabolites. Then, we cloned P450 cDNAs from the S401 cells treated with 2,4-D by RT-PCR methods. Among four novel P450 cDNA clones, CYP7IA11 and CYP81B2 were each expressed in the yeast Saccharomyces cerevisiae and were found to catalyze both ring-methyl hydroxylation and N-demethylation of chlortoluron, and ring-methylhydroxylation, respectively. It was also found that several mammalian P450 species including human P4501A1 metabolized chlortoluron through ring-methyl hydroxylation and N-dimethylation, and atrazine through N-dealkylation. When rat P4501A1 (CYP1A1) and its fused enzyme with yeast P450 reductase were each expressed in potato and tobacco plants, respectively, both transgenic plants showed resistance to the herbicides chlortoluron. Expression of a mammalian P450 species with overlapping broad substrate-specificity towards xenobiotics in plants seems to produce transgenic plants not only resistant to herbicides but also enhanced in metabolism of various environmental contaminants.

    DIAGNOSIS PRESS LTD, 1998年, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 12 (2), 17 - 22, 英語

    記事・総説・解説・論説等(学術雑誌)

  • Gene engineering for herbicide-resistant tobacco plants expressing mammalian p450 mono oxygenases

    H Ohkawa, N Shiota, H Inui, M Sugiura

    DIAGNOSIS PRESS LTD, 1996年, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 10 (4), 120 - 122, 英語

    記事・総説・解説・論説等(学術雑誌)

書籍等出版物

  • 1−7バレイショの形質転換プロトコール pp.64-70

    乾秀之

    共著, 形質転換プロトコール【植物編】、(株)化学同人, 2012年, 日本語

    学術書

  • 第2章ファイトレメディエーション:植物による土壌浄化技術、6 残留農薬のファイトレメディエーションとポリ塩化ビフェニル(PCB)などのファイトモニタリング pp.92-99

    大川秀郎, 乾秀之, 嶋津小百合

    共著, 植物機能のポテンシャルを活かした環境保全・浄化技術ー地球を救う超環境適合・自然調和型システムー、(株)シーエムシー出版, 2011年, 日本語

    学術書

  • Phytoremediation with genetically modified plants pp.339-354

    Yamazaki, K, Inui, H

    共著, Trends in Bioremediation and Phytoremediation, Research Signpost, 2010年, 英語

    学術書

  • 5.植物のP450酵素系、5.1植物の二次代謝産物の生合成に関与するP450分子種 pp.217-224

    乾秀之, 大川秀郎

    共著, 「P450の分子生物学」、(株)講談社サイエンティフィク, 2009年, 日本語

    学術書

  • Bioassay for persistent organic pollutants in transgenic plants with Ah receptor and GUS reporter genes

    INUI Hideyuki, GION Keiko, UTANI Yasushi, OHKAWA Hideo

    共著, Pesticide Chemistry, 2007年, 英語

    学術書

  • 8章バイオテクノロジー、8.2環境負荷化学物質の浄化植物の開発、「環境保全型農業事典」 pp.681-686

    大川 秀郎, 乾 秀之, 大川 安信

    共著, 丸善株式会社, 2005年, 日本語

    学術書

  • 8章バイオテクノロジー、8.2環境負荷化学物質の浄化植物の開発、「環境保全型農業事典」 pp.681-686

    大川 秀郎, 乾 秀之, 大川 安信

    共著, 丸善株式会社, 2005年, 日本語

    学術書

  • Monitoring of endocrine disruptors in transgenic plants carrying aryl hydrocarbon receptor and estrogen receptor genes, New Discoveries in Agrochemicals, Ed. by J. M. Clark and H. Ohkawa, pp.40-47

    INUI Hideyuki, SASAKI Hideaki, KODAMA Susumu, CHUA Nam-Hai, OHKAWA Hideo

    共著, American Chemical Society, 2005年, 英語

    学術書

  • Monitoring of endocrine disruptors in transgenic plants carrying aryl hydrocarbon receptor and estrogen receptor genes, New Discoveries in Agrochemicals, Ed. by J. M. Clark and H. Ohkawa, pp.40-47

    INUI Hideyuki, SASAKI Hideaki, KODAMA Susumu, CHUA Nam-Hai, OHKAWA Hideo

    共著, American Chemical Society, 2005年, 英語

    学術書

  • 第5節農薬等負荷軽減作物 「植物代謝工学ハンドブック」

    大川 秀郎, 乾 秀之

    共著, (株)エス・ティー・エス, 2002年, 日本語

    学術書

  • 第5節農薬等負荷軽減作物 「植物代謝工学ハンドブック」

    大川 秀郎, 乾 秀之, 今石 浩正

    共著, 株式会社エス・ティ・エス, 2002年, 日本語

    学術書

  • 代謝酵素:P450

    乾 秀之, 辻井 久恵, 大川 秀郎

    共著, 雑草科学実験法, 2001年, 日本語

    学術書

  • 基礎生化学実験法 第3巻タンパク質 II. 機能・動態解析法

    乾 秀之, 今石 浩正, 大川 秀郎

    その他, 日本生化学会編, 2001年, 日本語, タンパク質の発現、植物での発現

    学術書

  • 遺伝子組換え作物

    乾 秀之, 大川 秀郎

    共著, 農薬学事典, 2001年, 日本語

    学術書

  • タンパク質の発現、植物での発現

    乾 秀之, 今石 浩正, 大川 秀郎

    共著, 基礎生化学実験法 第3巻タンパク質II機能・動態解析法, 2001年, 日本語

    学術書

  • Herbicide Resistant Transgenic Plants Expressing Cytochrome P450 Monooxygenases Metabolizing Xenobiotics

    OHKAWA Hideo, INUI Hideyuki, IMAJUKU Yoshiro, IMAISHI Hiromasa

    共著, Agrochemical Discovery: Insect, Weed, and Fungal Control, 2001年, 英語

    学術書

  • ファイトレメディエーションを目指した薬物代謝型P450発現植物の作出

    大川 秀郎, 乾 秀之, 大川 安信

    共著, 植物と微生物による環境修復, 2000年, 日本語

    学術書

  • Cytochrome P450s and other Xenobiotic Metabolizing Enzymes in Plants

    OHKAWA Hideo, IMAISHI Hiromasa, SHIOTA Noriaki, YAMADA Takashi, INUI Hideyuki

    共著, Pesticide Chemistry and Bioscience: The Food-Environment Challenge, 1999年, 英語

    学術書

  • HERBICIDE-RESISTANT TOBACCO AND POTATO PLANTS EXPRESSING MAMMALIAN P450 MONOOXYGENASES

    OHKAWA Hideo, SHIOTA Noriaki, INUI Hideyuki, SUGIURA Miwa, YABUSAKI Yoshiyasu, OHKAWA Yasunobu, ISHIGE Teruo

    共著, Regulation of Enzymatic Systems Detoxifying Xenobiotics in Plants, 1997年, 英語

    学術書

講演・口頭発表等

  • Selection of biomarker genes from the brown alga Ectocarpus siliculosus, treated with antifouling agents

    乾 秀之, 安田 諒子, 川井 浩, Vladimir Beškoski, 岡村 秀雄

    The 16th International Symposium on Persistent Toxic Substances, 2019年10月, 英語, 国際会議

    ポスター発表

  • Biochemical and molecular characterization of Klebsiella sp. as a potential agent for removal of perfluoroalkyl substances from the environment

    Aleksandra Žerađanin, Kristina Joksimović, Jelena Avdalović, Nikoleta Lugonja, Takeshi Nakano, 乾 秀之, Vladimir Beškoski

    13th Symposium with international participation “NOVEL TECHNOLOGIES AND ECONOMIC DEVELOPMENT”, 2019年10月, 英語, 国際会議

    口頭発表(一般)

  • 海藻類の増殖に及ぼす防汚剤の有害影響

    岡村秀雄, 安田諒子, 乾 秀之, Vladimir Beškoski, 川井浩史

    第25回日本環境毒性学会研究発表会, 2019年09月, 日本語, 国内会議

    ポスター発表

  • ポリ塩化ビフェニル汚染と土壌微生物の関連性の解析

    櫻間晴子, 澤誠人, 羽賀雄紀, 松村千里, 乾 秀之, 中野武, 萩下大郎

    日本農芸化学会関西・中部支部2019年度合同神戸大会, 2019年09月, 日本語, 国内会議

    口頭発表(一般)

  • Survey of the bacterial community in polychlorinated biphnyl-polluted environment

    櫻間 晴子, 澤 誠人, 羽賀 雄紀, 松村 千里, 乾 秀之, 中野 武

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    ポスター発表

  • Structural basis of metabolism of chiral polychlorinated biphenyls by mammalian cytochrome P450 monooxygenases

    乾 秀之, 伊藤 輝志, 羽賀 雄紀, 久保 惇, 伊藤 俊将, 山本 恵子, 松村 千里, 中野 武

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    ポスター発表

  • Structural basis of CB118 metabolism by cytochrome P450 monooxygenase (P450BM3) mutants from Bacillus megaterium

    石田 裕子, 後藤 絵里香, 羽賀 雄紀, 久保 惇, 伊藤 俊将, 笠井 千枝, 荘司 長三, 山本 恵子, 松村 千里, 中野 武, 乾 秀之

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    ポスター発表

  • Reduction of POP contamination in Cucurbitaceae family focusing on the transporting factors for POPs by the treatment of pesticides

    藤田 健太郎, 近藤 恭光, 本田 香織, 長田 裕之, 乾 秀之

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    口頭発表(一般)

  • Metabolism of polychlorinated naphthalenes by mammalian cytochrome P450 monooxygenases

    都築 治延, 伊藤 輝志, 羽賀 雄紀, 松村 千里, 中野 武, 乾 秀之

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    ポスター発表

  • Increased sensitivity of transgenic tobacco plants carrying transporting factors toward POPs

    Petya Stoykova, 乾 秀之

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    ポスター発表

  • Biodegradation of selected PCBs by microorganisms isolated from PCBs polluted environment

    Vladimir Beškoski, Aleksandra Žeradjanin, Gordana Gojgić-Cvijović, Srđan Miletić, Jelena Avdalović, Yuki Haga, 乾 秀之, Chisato Matsumura, Takeshi Nakano

    39th International Symposium on Halogenated Persistent Organic Pollutants, 2019年08月, 英語, 国際会議

    ポスター発表

  • 汚染物質体内輸送因子によるウリ科植物の作物汚染

    藤田 健太郎, 乾 秀之

    第2回天然ゴム研究会, 2019年07月, 日本語, 国内会議

    ポスター発表

  • 哺乳動物シトクロムP450モノシゲナーゼによるキラルポリ塩化ビフェニルの代謝とその構造基盤

    伊藤 輝志, 羽賀 雄紀, 久保 惇, 伊藤 俊将, 山本 恵子, 松村 千里, 中野 武, 乾 秀之

    第28回環境化学討論会, 2019年06月, 日本語, 埼玉, 国内会議

    口頭発表(一般)

  • 哺乳動物シトクロムP450モノオキシゲナーゼによるポリ塩化ナフタレンの代謝

    乾 秀之, 都築 治延, 伊藤 輝志, 羽賀 雄紀, 松村 千里, 中野 武

    第28回環境化学討論会, 2019年06月, 日本語, 国内会議

    ポスター発表

  • 河川底質におけるペルフルオロアルキル酸分解菌の探索

    小林 豊, 吉識 亮介, 櫻間 晴子, 山本 敦史, 松村 千里, 中野 武, 乾 秀之

    第28回環境化学討論会, 2019年06月, 日本語, 国内会議

    ポスター発表

  • キャピラリーゾーン電気泳動法による塩中フッ化物イオンの定量

    福士 惠一, 堀田 弘樹, 本健 嗣, 岡村 秀雄, 乾 秀之

    2019年度日本海水学会第70年会, 2019年06月, 日本語, 国内会議

    口頭発表(一般)

  • Transgenic Arabidopsis lines for monitoring of low concentrations of endocrine disruptors

    Petya Stoykova, 乾 秀之

    第28回環境化学討論会, 2019年06月, 英語, 国内会議

    口頭発表(一般)

  • Bacillus megaterium由来シトクロムP450モノオキシゲナーゼP450BM3変異体によるCB118の代謝とその構造基盤

    石田 裕子, 後藤 絵里香, 羽賀 雄紀, 久保 惇, 伊藤 俊将, 笠井 千枝, 荘司 長三, 山本 恵子, 松村 千里, 中野 武, 乾 秀之

    第28回環境化学討論会, 2019年06月, 日本語, 国内会議

    ポスター発表

  • ポリ塩化ビフェニル類汚染化による土壌微生物生態系への影響評価

    櫻間晴子, 比良岡, 山下舞, 澤誠人, 羽賀雄紀, 松村千里, 乾 秀之, 中野武, 萩下大郎

    2019年度日本農芸化学会, 2019年03月, 日本語, 国内会議

    口頭発表(一般)

  • ズッキーニにおいて同定された汚染物質輸送因子の病原抵抗性への関与

    藤田健太郎, 乾 秀之

    2019年度日本農芸化学会, 2019年03月, 日本語, 国内会議

    口頭発表(一般)

  • Environmental behavior of PCBs and its enantioselective toxicity

    Takeshi Nakano, 乾 秀之

    Pure and Applied Chemistry International Conference 2019, 2019年02月, 英語, 国際会議

    ポスター発表

  • 汚染物質輸送因子に着目した農薬による作物汚染の低減化

    藤田健太郎, 近藤恭光, 本田香織, 長田裕之, 乾 秀之

    若手フロンティア研究会2018, 2018年12月, 日本語, 国内会議

    ポスター発表

  • キャピラリーゾーン電気泳動法による海水中フッ化物イオンの定量

    福士惠一, 堀田弘樹, 林美鶴, 乾 秀之, 辻本淳一

    第4回海洋環境研究集会, 2018年12月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物におけるPOPs輸送タンパク質の細胞内局在と分泌

    諏訪瑞季, 乾 秀之

    若手フロンティア研究会2018, 2018年12月, 日本語, 国内会議

    ポスター発表

  • 植物の農薬取り込みメカニズムを利用した農薬の新規利用法

    乾 秀之

    第16回農薬バイオサイエンス研究会, 2018年11月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物におけるPOPs輸送タンパク質の細胞内局在と分泌

    諏訪 瑞季, 岩渕 彩, 池田 健一, 乾 秀之

    日本農薬学会第36回農薬環境科学研究会, 2018年11月, 日本語, 甲府、山梨県, 国内会議

    ポスター発表

  • ウリ科植物におけるPOPs輸送タンパク質の細胞内局在と分泌

    諏訪瑞季, 岩渕彩, 池田健一, 乾 秀之

    第36回農薬環境科学研究会, 2018年11月, 日本語, 国内会議

    ポスター発表

  • Uptake mechanism of persistent organic pollutants by plants and its application to phytoremediation and crop safety

    Hideyuki Inui

    Environmental Protection, - Prevention, Monitoring and Remediation – Worldwide and Our Experiences, 2018年11月, 英語, 国際会議

    口頭発表(一般)

  • DNA二本鎖切断修復機構がRhizobium radiobacterによる植物染色体へのDNA挿入に与える影響

    岡田真希, 三富優香, 田上舞, 乾 秀之, 田中秀逸, 吉原亮平

    日本遺伝学会第90回大会, 2018年09月, 日本語, 国内会議

    ポスター発表

  • 沿岸域の汚染を評価するための海藻類増殖阻害試験法の開発

    岡村秀雄, 安田諒子, 乾 秀之, 川井浩史

    瀬戸内海研究フォーラムin兵庫, 2018年08月, 英語, 国内会議

    ポスター発表

  • Transient ITP-CZE Determination of Fluoride in Saline Samples

    福士惠一, 辻本淳一, 服部考成, 堀田弘樹, 松本健嗣, 乾秀之

    25th International Symposium on Electro- and Liquid Phase-Separation Techniques, 2018年08月, 英語, 京都大学桂, 国際会議

    ポスター発表

  • Reduction of crop contamination resulting from hydrophobic contaminants by the treatment of pesticides targeting to its transport factors

    Kentaro Fujita, Aoi Sakai, Yasumitsu Kondoh, Kaori Honda, Hiroyuki Osada, Hideyuki Inui

    38th International Symposium on Halogenated Persistent Organic Pollutants, 2018年08月, 英語, 国際会議

    ポスター発表

  • Enhanced metabolism of 2,3',4,4',5-pentachlorobiphenyl (CB118) by bacterial cytochrome P450 monooxygenase mutants

    Hideyuki Inui, Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano

    38th International Symposium on Halogenated Persistent Organic Pollutants, 2018年08月, 英語, 国際会議

    ポスター発表

  • キャピラリーゾーン電気泳動法による高塩分試料中フッ化物イオンの定量

    福士惠一, 辻本淳一, 服部考成, 堀田弘樹, 乾秀之

    日本海水学会第69年会, 2018年06月, 日本語, 日本海水学会, 千葉大学, 国内会議

    ポスター発表

  • 高脂溶性汚染物質によって引き起こされる作物汚染の化合物輸送因子を標的とした農薬による低減化

    藤田健太郎, 近藤恭光, 本田香織, 長田裕之, 乾 秀之

    日本農薬学会第43回大会, 2018年05月, 日本語, 国内会議

    口頭発表(一般)

  • 微生物由来P450モノオキシゲナーゼによるPCBの代謝に影響を及ぼす有機フッ素化合物

    乾 秀之

    公立鳥取環境大学セミナー, 2018年03月, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Bacillus megaterium由来シトクロムP450モノオキシゲナーゼによる2,3',4,4',5-ペンタクロロビフェニル(CB118)の代謝とその構造基盤

    後藤絵里香, 羽賀雄紀, 久保惇, 伊藤俊将, 笠井千枝, 荘司長三, 山本恵子, 松村千里, 中野武, 乾 秀之

    2018年度日本農芸化学会, 2018年03月, 日本語, 国内会議

    口頭発表(一般)

  • 動物が持つ化学物質応答メカニズムを利用した汚染物質のファイトモニタリング

    乾 秀之

    第五回バイオシグナル研究会, 2017年10月, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Possible metabolic fates of polychlorinated biphenyls by soil bacterium under complex pollution and its structural basis

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

    The 14th International Symposium on Persistent Toxic Substances, 2017年09月, 英語, 国内会議

    ポスター発表

  • Localization of major latex-like proteins is important for contamination of Cucurbita pepo by persistent organic pollutants

    Mizuki Suwa, Aya Iwabuchi, Kiyoshi Yamazaki, Kenichi Ikeda, Hideyuki Inui

    The 14th International Symposium on Persistent Toxic Substances, 2017年09月, 英語, 国内会議

    ポスター発表

  • Localization of major latex-like proteins is important for contamination of Cucurbita pepo by persistant organic pollutants

    諏訪 瑞季, 岩渕 彩, 山崎 清志, 池田 健一, 乾 秀之

    The 14th International Symposium on Persistent Toxic Substances 2017, 2017年09月, 英語, Nagoya, Japan, 国際会議

    ポスター発表

  • Involvement of transporting factors for persistent organic pollutants in Cucurbita pepo toward stress responses

    Erika Isono, Soichiro Asuke, Yukio Tosa, Hideyuki Inui

    The 14th International Symposium on Persistent Toxic Substances, 2017年09月, 中国語, 国内会議

    ポスター発表

  • Distribution of perfluoroalkyl acids from Osaka Bay to coastal waters of Western Japan

    Hideyuki INUI, Katsuya YAMAMOTO, Atsushi YAMAMOTO, Hideo OKAMURA, Mitsuru HAYASHI, Takeshi NAKANO, Chisato MATSUMURA, Keiichi FUKUSHI, Vladimir, p. BESKOSKI

    The 14th International Symposium on Persistent Toxic Substances, 2017年09月, 英語, Nagoya(Aichi), 国際会議

    口頭発表(一般)

  • Structural basis of CB118 metabolism by bacterial cytochrome P450 monooxygenase and its mutants with perfluorocarboxylic acids

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

    37th International Symposium on Halogenated Persistent Organic Pollutants, 2017年08月, 英語, 国際会議

    ポスター発表

  • Metabolism of chiral polychlorinated biphenyls by mammalian cytochrome P450 monooxygenases

    Terushi Ito, Chiharu Miwa, Yuki Haga, Erika Goto, Toshimasa Itoh, Keiko Yamamoto, Shintaro Mise, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

    37th International Symposium on Halogenated Persistent Organic Pollutants, 2017年08月, 英語, 国際会議

    ポスター発表

  • Crucial steps for crop contamination by persistent organic pollutants in Cucurbitaceae family

    諏訪 瑞季, 岩渕 彩, 池田 健一, 乾 秀之

    DIOXIN 2017, 2017年08月, 英語, Vancouver, Canada, 国際会議

    ポスター発表

  • Crucial steps for crop contamination by persistent organic pollutants in Cucurbitaceae family

    Mizuki Suwa, Aya Iwabuchi, Kenichi Ikeda, Hideyuki Inui

    37th International Symposium on Halogenated Persistent Organic Pollutants, 2017年08月, 英語, 国際会議

    ポスター発表

  • A major latex-like protein is a determinative factor for contamination of persistent organic pollutants in Cucurbitaceae family

    Hideyuki Inui, Mizuki Suwa, Aya Iwabuchi

    37th International Symposium on Halogenated Persistent Organic Pollutants, 2017年08月, 英語, 国際会議

    ポスター発表

  • 大阪湾から西日本の沿岸海域に拡がるペルフルオロアルキル酸

    乾 秀之, 山本勝也, 山本敦史, 岡村秀雄, 林美鶴, 中野武, 松村千里, 福士惠一, 和田真平, Vladimir P. Beškoski

    第26回環境化学討論会, 2017年06月, 日本語, 国内会議

    ポスター発表

  • 高脂溶性汚染物質によって引き起こされる作物汚染の農薬による低減

    乾 秀之, 田中澪月, 中島一成, 野本友愛, 三木碩己, 藤田健太郎

    日本農薬学会第42回大会, 2017年03月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物が持つ汚染物質輸送因子のストレス応答への関与

    礒野絵梨香, 足助聡一郎, 土佐幸雄, 乾 秀之

    2017年度日本農芸化学会, 2017年03月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物の秘めたる力 〜汚染物質の吸収と輸送〜

    乾 秀之

    公益財団法人兵庫工業会 兵庫バイオテクノロジー研究会 第98回定例会, 2017年02月, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 微生物由来P450モノオキシゲナーゼによるPCBの代謝に影響を及ぼす有機フッ素化合物

    乾 秀之

    13回PCB講演会, 2017年01月, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • デコイ分子を用いたBacillus megaterium由来シトクロムP450モノオキシゲナーゼによるCB118の代謝とその構造基盤

    後藤絵里香, 乾 秀之

    若手フロンティア研究会2016, 2016年12月, 日本語, 国内会議

    ポスター発表

  • ウリ科植物の汚染物質輸送因子によるPCB同族体の選択的蓄積

    礒野絵梨香, 乾 秀之

    若手フロンティア研究会2016, 2016年12月, 日本語, 国内会議

    ポスター発表

  • Structural determinants of species differences on metabolism of dioxin-like polychlorinated biphenyls by mammalian cytochrome P450 monooxygenases

    Hideyuki Inui, Yuki Haga, Toshimasa Itoh, Erika Goto, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki

    The 9th International PCB Workshop, 2016年10月, 英語, 国内会議

    口頭発表(一般)

  • Metabolic fates of CB118 by cytochrome P450 monooxygenase from soil bacterium under complex pollution and its structural basis

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

    The 9th International PCB Workshop, 2016年10月, 英語, 国内会議

    ポスター発表

  • Congener-specific uptake of polychlorinated biphenyls by transporting factors in Cucurbita pepo

    Erika Isono, Hideyuki Inui

    The 9th International PCB Workshop, Kobe, 2016年10月, 英語, 国内会議

    ポスター発表

  • Structural basis of CB118 metabolism by cytochrome P450 monooxygenase from Bacillus megaterium with decoy molecules

    Erika Goto, Yuki Haga, Makoto Kubo, Toshimasa Itoh, Chie Kasai, Osami Shoji, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Hideyuki Inui

    The 13th International Symposium on Cytochrome P450 Biodiversity & Biotechnology, 2016年07月, 英語, 国際会議

    ポスター発表

  • Enantioselective metabolism of chiral polychlorinated biphenyls by rat and human cytochrome P450 monooxygenases

    Hideyuki Inui, Chiharu Miwa, Shintaro Mise, Erika Goto, Yuki Haga, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki

    The 13th International Symposium on Cytochrome P450 Biodiversity & Biotechnology, 2016年07月, 英語, 国際会議

    ポスター発表

  • デコイ分子を用いたBacillus megaterium由来シトクロムP450モノオキシゲナーゼによるCB118の代謝とその構造基盤

    後藤絵里香, 羽賀雄紀, 久保惇, 伊藤俊将, 笠井千枝, 荘司長三, 山本恵子, 松村千里, 中野武, 乾 秀之

    第25回環境化学討論会, 2016年06月, 日本語, 国内会議

    口頭発表(一般)

  • ズッキーニにおけるダイオキシン類の体内輸送に関わる遺伝子の発現調節機構

    乾 秀之, 吉原亮平, 廣田又士, 後藤純弥, 殷熙洙

    2016年度日本農芸化学会, 2016年03月, 日本語, 国内会議

    ポスター発表

  • ウリ科植物が持つ脂溶性汚染物質輸送タンパク質の結合特性の比較

    岩渕彩, 勝手野乃香, 後藤純弥, 乾 秀之

    日本農薬学会第41回大会, 2016年03月, 日本語, 国内会議

    口頭発表(一般)

  • Characterization of Klebsiella variicola, environmental isolate which can reduce concentration of perfluoroalkyl compounds in laboratory tests

    Aleksandra Djurić, Gordana Gojgić-Cvijović, Dragica Jakovljević, Atsushi Yamamoto, Hideyuki Inui, Takeshi Nakano, Vladimir Beškoski

    Belgrade International Molecular Life Science Conference for Students, 2016年02月, 英語, 国際会議

    ポスター発表

  • ウリ科植物による脂溶性汚染物質の取り込み・蓄積メカニズム

    岩渕彩, 乾 秀之

    若手フロンティア研究会2015, 2015年12月, 日本語, 国内会議

    ポスター発表

  • ウリ科植物による脂溶性汚染物質の取り込み・蓄積メカニズム

    岩渕彩, 乾 秀之

    第1回植物の栄養研究会, 2015年09月, 日本語, 国内会議

    ポスター発表

  • 分析対象を限定しない高分解能質量分析による PFOS 生分解生成物の探索

    山本敦史, Vladimir P. Beškoski, 山本勝也, 乾秀之, 川﨑英也, 荒川隆一

    第24回環境化学討論会, 2015年06月, 日本語, 札幌, 国内会議

    ポスター発表

  • ラット及びヒト由来シトクロムP450モノオキシゲナーゼによるキラルポリ塩化ビフェニルの代謝

    乾秀之, 三輪千晴, 見世慎太朗, 後藤絵里香, 羽賀雄紀, 松村千里, 中野武

    第24回環境化学討論会, 2015年06月, 日本語, 札幌, 国内会議

    ポスター発表

  • Transport mechanisms of compounds in plants

    Hideyuki Inui

    Seminar at AgroBioInstitute, 2015年06月, 英語, Sofia; Bulgaria, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Structural basis of species differences on metabolism and toxicity of polychlorinated biphenyls among mammals

    Hideyuki Inui

    Seminar at University of Belgrade, 2015年06月, 英語, Belgrade; Serbia, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Phytoremediation of persistent organic pollutants: Lesson from zucchini and pumpkin

    Hideyuki Inui

    7th Symposium: Chemistry and Environmental Protection - EnviroChem 2015, 2015年06月, 英語, Palić; Serbia, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Comparison of transporting factors for persistent organic pollutants in Cucurbitaceae family

    Aya Iwabuchi, Junya Goto, Nonoka Katte, Hideyuki Inui

    7th Symposium: Chemistry and Environmental Protection - EnviroChem 2015, 2015年06月, 英語, Palić; Serbia, 国際会議

    口頭発表(一般)

  • Biotransformation of Perfluorooctanesulfonate (PFOS) and Perfluorooctanoate (PFOA) by the Action of Microbial Community Isolated From Polluted Environment - First Step Towards Successful Bioremediation

    Vladimir P. Beškoski, Takeshi Nakano, Atsushi Yamamoto, Chisato Matsumura, Katsuya Yamamoto, Hideyuki Inui

    7th Symposium: Chemistry and Environmental Protection - EnviroChem 2015, 2015年06月, 英語, Palić; Serbia, 国際会議

    口頭発表(一般)

  • 植物によるPCBの吸収メカニズム

    乾秀之

    第2回PCB講演会, 2015年05月, 日本語, 大阪, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 哺乳類シトクロムP450モノオキシゲナーゼによるポリ塩化ビフェニルの毒性低下メカニズム

    見世慎太朗, 加藤晃, 伊藤俊将, 山本恵子, 乾秀之

    日本農芸化学会大会, 2015年03月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • ウリ科植物における残留性有機汚染物質の地上部移行メカニズム.

    岩渕 彩, 後藤 純弥, 吉原 亮平, 池田 健一, 井上 加奈子, 乾 秀之

    日本農芸化学会大会, 2015年03月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • ウリ科植物における残留性有機汚染物質の取り込みに影響を与える環境要因とそのメカニズム

    勝手野乃香, 後藤純弥, 岩渕彩, 乾秀之

    日本農薬学会, 2015年03月, 日本語, 玉川大学, 国内会議

    口頭発表(一般)

  • Biotransformation of perfluorooctanoic acid by the action of microbial consortia isolated from sediment polluted with perfluorinated compounds

    Vladimir P. Beškoski, Hideyuki Inui, Atsushi Yamamoto, Chisato Matsumura, Katsuya Yamamoto, Takeshi Nakano

    Contaminated Sediments: Environmental Chemistry; Ecotoxicology and Engineering, 2015年03月, 英語, Ascona; Switzerland, 国際会議

    口頭発表(一般)

  • Uptake mechanism of persistent organic pollutants by Cucurbitaceae family

    Hideyuki Inui, Junya Goto, Aya Iwabuchi, Ryouhei Yoshihara, Heesoo Eun

    International Conference of Asian Environmental Chemistry 2013, 2014年11月, 英語, Bangkok; Thailand, 国際会議

    口頭発表(一般)

  • Structural basis of metabolism of 2;3';4;4';5-pentachlorobiphenyl (CB118) by mammalian cytochrome P450 monooxygenases

    Shintarou Mise, Yuuki Haga, Miku Yabu, Toshimasa Itoh, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hideyuki Inui

    International Conference of Asian Environmental Chemistry 2014, 2014年11月, 英語, Bangkok; Thailand, 国際会議

    ポスター発表

  • Interaction of PFOS; PFOA and 8:2 FTOH with human; rat; and microbial cytochrome P450s: Similarities and differences

    Vladimir Beškoski, Takeshi Nakano, Chisato Matsumura, Katsuya Yamamoto, Atsushi Yamamoto, Mamoru Motegi, Hideo Okamura, Hideyuki Inui

    International Conference of Asian Environmental Chemistry 2014, 2014年11月, 英語, Bangkok; Thailand, 国際会議

    ポスター発表

  • Function and dynamic of transporting factors for persistent organic pollutants in Cucurbita pepo

    Junya Goto, Aya Iwabuchi, Hideyuki Inui

    International Conference of Asian Environmental Chemistry 2014, 2014年11月, 英語, Bangkok; Thailand, 国際会議

    ポスター発表

  • Biotransformation of perfluorinated compounds by the action of microbial community isolated from polluted environment - road to successful bioremediation

    Vladimir P. Beškoski, Takeshi Nakano, Atsushi Yamamoto, Chisato Matsumura, Katsuya Yamamoto, Mamoru Motegi, Hideo Okamura, Hideyuki Inui

    International Conference of Asian Environmental Chemistry 2014, 2014年11月, 英語, Bangkok; Thailand, 国際会議

    ポスター発表

  • Mammalian cytochrome P450-dependent metabolism of polychlorinated dibenzo-p-dioxins and coplanar polychlorinated biphenyls; and application to bioremediation

    Hideyuki Inui, Shintarou Mise, Toshimasa Itoh, Keiko Yamamoto, Shin-Ichi Ikushiro, Toshiyuki Sakaki

    The 12th International Symposium on Cytochrome P450 Biodiversity & Biotechnology, 2014年09月, 英語, Kyoto, 国際会議

    ポスター発表

  • 各種ウリ科植物が持つ残留性有機汚染物質の体内輸送因子の比較

    岩渕彩, 後藤純弥, 乾秀之

    第23回環境化学討論会, 2014年05月, 日本語, 京都大学, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Structural Basis of Species Differences in Metabolism of Polychlorinated Biphenyls

    Hideyuki Inui

    23nd Symposium on Environmental Chemistry; Night meeting, 2014年05月, 英語, Kyoto, 国際会議

    口頭発表(一般)

  • ズッキーニにおけるダイオキシン類の取り込みに係わる遺伝子の機能解析

    乾秀之, 廣田又士, 後藤純弥, 吉原亮平, 兒玉典子, 松井智美, 山﨑清志, 殷煕洙

    日本農芸化学会2015年度大会, 2014年03月, 日本語, 明治大学, 国内会議

    口頭発表(一般)

  • ウリ科植物の根における脂溶性物質結合タンパク質の局在性.

    吉原亮平, 後藤純弥, 岩淵彩, 池田 健一, 井上加奈子, 乾 秀之

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 明治大学, 国内会議

    口頭発表(一般)

  • ウリ科植物における残留性有機汚染物質の体内輸送因子の機能・動態

    後藤純弥, 岩渕彩, 乾秀之

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 明治大学, 国内会議

    口頭発表(一般)

  • ウリ科植物における残留性有機汚染物質の取り込みメカニズム

    乾秀之, 沢田真美, 後藤純弥, 山﨑清志, 兒玉典子, 鶴田宏樹, 殷熙洙

    日本農薬学会, 2014年03月, 日本語, 京都大学, 国内会議

    口頭発表(一般)

  • ウリ科植物が持つダイオキシン類蓄積性能に係わる因子の同定

    廣田 又士, 後藤 純弥, 吉原 亮平, 殷 煕洙, 乾 秀之

    日本農芸化学会関西支部第483回講演会, 2014年02月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • Structural Basis of Species Differences between Rat and Human CYP1A1s in Metabolism of Polychlorinated Biphenyls

    Hideyuki Inui

    e-symposium, 2014年02月, 英語, Osaka, 国内会議

    口頭発表(招待・特別)

  • Amino acid variations in mammalian cytochrome P450 monooxygenases responsible for decrease of dioxin-like toxicity of polychlorinated biphenyls

    Hideyuki Inui, Kiyoshi Yamazaki, Miku Yabu, Toshimasa Itoh, Keiko Yamamoto, Motoharu Suzuki, Yuuki Haga, Shintarou Mise, Masahiro Tsurukawa, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki

    33nd International Symposium on Halogenated Persistent Organic Pollutants, 2013年08月, 英語, Daegu; Korea, 国際会議

    ポスター発表

  • ラットおよびヒト由来シトクロムP450モノオキシゲナーゼによるCB118の代謝と3次元構造の比較

    見世慎太朗, 羽賀雄紀, 薮未来, 伊藤俊将, 山本恵子, 鶴川正寛, 松村千里, 中野武, 榊利之, 乾秀之

    第22回環境化学討論会, 2013年07月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • Structural Basis of Species Differences between Rat and Human CYP1A1s in Metabolism of Polychlorinated Biphenyls

    Hideyuki Inui

    22nd Symposium on Environmental Chemistry, 2013年07月, 英語, Tokyo, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • n planta観察法を用いた根における親水性及び疎水性化合物の取り込み経路の解明

    山﨑清志, 鶴田宏樹, 乾秀之

    日本農薬学会第38回大会, 2013年03月, 日本語, つくば, 国内会議

    口頭発表(一般)

  • Hydrophilic and hydrophobic pathways for the uptake of compounds in lateral roots based on in planta observation

    Kiyoshi Yamazaki, Hiroki Tsuruta, Hideyuki Inui

    International Workshop on Plant Membrane Biology XVI, 2013年03月, 英語, Kurashiki, 国際会議

    ポスター発表

  • 抗体可変領域を用いた抗体転写因子による脂溶性物質の細胞膜透過性の評価

    河野実織, 櫻井由季, 祇園景子, 乾秀之

    第85回日本生化学会大会, 2012年12月, 日本語, 福岡, 国内会議

    ポスター発表

  • ポリ塩化ビフェニル代謝能に影響を及ぼすラットおよびヒトCYP1A1の基質結合部位を構成するアミノ酸の同定

    薮未来, 羽賀雄紀, 山﨑清志, 鈴木元治, 伊藤俊将, 山本恵子, 鶴川正寛, 松村千里, 中野武, 榊利之, 乾秀之

    第85回日本生化学会大会, 2012年12月, 日本語, 福岡, 国内会議

    ポスター発表

  • Structural basis of species differences between rat and human CYP1A1s in metabolism of polychlorinated biphenyls

    Miku Yabu, Kiyoshi Yamazaki, Motoharu Suzuki, Yuki Haga, Toshimasa Itoh, Keiko Yamamoto, Masahiro Tsurukawa, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hideyuki Inui

    チトクロムP450発見50周年記念シンポジウム, 2012年12月, 日本語, 福岡, 国内会議

    ポスター発表

  • ウリ科植物に学ぶ脂溶性汚染物質の環境浄化メカニズム

    乾秀之

    創造例会2012;はりま産学交流会, 2012年11月, 日本語, 姫路, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • ウリ科植物に学ぶ脂溶性汚染物質の環境浄化メカニズム

    乾秀之

    第10回積水化学自然に学ぶものづくりフォーラム, 2012年10月, 日本語, 東京, 国内会議

    ポスター発表

  • High accumulation and congener specific uptake of dioxins and dioxin-like compounds in Cucurbita pepo

    Ryouhei Yoshihara, Kiyoshi Yamazaki, Heesoo Eun, Hideyuki Inui

    SETAC Asia/Pacific 2012, 2012年09月, 英語, Kumamoto, 国際会議

    口頭発表(一般)

  • Crucial factors in species differences on metabolism of polychlorinated biphenyls by rat and human CYP1A1s

    Miku Yabu, Kiyoshi Yamazaki, Motoharu Suzuki, Yuki Haga, Toshimasa Ito, Keiko Yamamoto, Masahiro Tsurukawa, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hideyuki Inui

    SETAC Asia/Pacific 2012, 2012年09月, 英語, Kumamoto, 国際会議

    ポスター発表

  • Selective uptake of polychlorinated biphenyls by Cucurbita pepo

    Hideyuki Inui, Kiyoshi Yamazaki, Shinryo, Matsuo, Ryouhei Yoshihara, Heesoo Eun

    32nd International Symposium on Halogenated Persistent Organic Pollutants; Cairns, 2012年08月, 英語, Cairns; Australia, 国際会議

    ポスター発表

  • 残留性有機汚染物質によるウリ科植物の汚染を引き起こす遺伝子の同定

    廣田又士, 吉原亮平, 兒玉典子, 殷熙洙, 山﨑清志, 乾秀之

    第21回環境化学討論会, 2012年07月, 日本語, 松山, 国内会議

    口頭発表(一般)

  • 植物の根による脂溶性化合物の吸収経路

    山﨑清志, 鶴田宏樹, 乾秀之

    日本農薬学会第37回大会, 2012年03月, 日本語, 岡山, 国内会議

    口頭発表(一般)

  • ズッキーニによるダイオキシン類の取り込みに係わる遺伝子の同定

    乾秀之, 廣田又士, 山﨑清志

    2012年度日本農芸化学会, 2012年03月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • 抗体可変領域を用いた抗体転写因子による脂溶性物質の細胞膜透過性の評価

    河野実織, 櫻井由季, 祇園景子, 乾秀之

    日本生化学会大会(Web), 2012年, 日本語, 国内会議

    ポスター発表

  • どんな物質でも誘導剤にできる抗体可変領域を用いた新規遺伝子発現調節システム

    乾秀之

    生物化学的測定研究会 第16回学術シンポジウム, 2011年11月, 日本語, 京都, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Congener specificity in the accumulation of dioxins and dioxin-like compounds in Cucurbita pepo grown hydroponically

    Hideyuki Inui, Taketo Wakai, Keiko Gion, Kiyoshi Yamazaki, Yun-Seok Kim, Heesoo Eun

    8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference, 2011年11月, 英語, Kobe, 国際会議

    ポスター発表

  • Accumulation mechanism of dioxins and dioxin-like compounds in Cucurbita pepo

    Kiyoshi Yamazaki, Shinryo Matsuo, Heesoo Eun, Hiroki Tsuruta, Hideyuki Inui

    8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference, 2011年11月, 英語, Kobe, 国際会議

    ポスター発表

  • ラットおよびヒトCYP1A1によるポリ塩化ビフェニルの代謝と3次元構造の比較

    薮未来, 山﨑清志, 鈴木元治, 羽賀雄紀, 伊藤俊将, 山本恵子, 鶴川正寛, 松村千里, 中野武, 榊利之, 乾秀之

    第20回環境化学討論会, 2011年07月, 日本語, 熊本, 国内会議

    口頭発表(一般)

  • Cucurbita pepoにおけるダイオキシン類の器官局在性

    松井智美, 松尾申遼, 殷熙洙, 山﨑清志, 乾秀之

    第20回環境化学討論会, 2011年07月, 日本語, 熊本, 国内会議

    口頭発表(一般)

  • 残留性有機汚染物質に対するウリ科植物の特異的な吸収・蓄積に及ぼす根滲出物の影響

    松尾申遼, 山﨑清志, 殷熙洙, 乾秀之

    日本農薬学会第36回大会, 2011年03月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • ヒト並びにラット CYP1A1 の3D モデルと PCB126 代謝

    山本恵子, 伊藤俊将, 山﨑清志, 乾秀之

    日本薬学会第131年会, 2011年03月, 日本語, 静岡, 国内会議

    口頭発表(一般)

  • シトクロムP450によるコプラナーポリ塩化ビフェニルの代謝とドッキングモデル

    乾秀之, 山﨑清志, 藪未来, 鈴木元治, 伊藤俊将, 山本恵子, 松村千里, 中野武, 榊利之

    2011年度日本農芸化学会, 2011年03月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • Promotion of tuberization by overexpression of Arabidopsis thaliana LOV KELCH REPEAT PROTEIN 2 in potato (Solanum tuberosum cv. May Queen)

    Yuuki Nishiyama, Hideyuki Inui, Yasunobu Ogura, Takayoshi Shimazaki, Akira Kikuchi, Tomoyuki Takase, Tomohiro Kiyosue

    Joint International Symposium on Japanese Solanaceae/Cucurbitaceae Genomics Initiatives, 2011年03月, 英語, Okayama, 国際会議

    ポスター発表

  • ウリ科植物における脂溶性汚染物質の体内移行に係わる因子

    沢田真美, 松尾申遼, 山﨑清志, 乾秀之

    日本農芸化学会関西支部第468回講演会, 2011年02月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • 組換え型AhR/GUSレポーター遺伝子系導入シロイヌナズナによるPCB同族体のアッセイにおけるバイオサーファクタントの効果

    嶋津小百合, 太田雅也, 乾秀之, 芦田均, 大川秀郎

    若手フロンティア研究会2010, 2010年12月, 日本語, 神戸, 国内会議

    ポスター発表

  • 抗体可変領域を用いた抗体転写因子の高感度化に影響を与えるドメイン配列

    河野実織, 櫻井由季, 祗園景子, 乾秀之

    第83回日本生化学会大会, 2010年12月, 日本語, 神戸, 国内会議

    ポスター発表

  • ラットおよびヒトCYP1A1による3;3';4;4'-テトラクロロビフェニルの代謝能の比較

    藪未来, 山﨑清志, 鈴木元治, 伊藤俊将, 山本恵子, 松村千里, 中野武, 榊利之, 乾秀之

    第83回日本生化学会大会, 2010年12月, 日本語, 神戸, 国内会議

    口頭発表(一般)

  • ドッキングモデルを用いたラットCYP1A1による3;3';4;4';5-ペンタクロロビフェニル代謝メカニズムの解明

    山﨑清志, 鈴木元治, 伊藤俊将, 山本恵子, 松村千里, 中野武, 榊利之, 今石浩正, 乾秀之

    第83回日本生化学会大会, 2010年12月, 日本語, 神戸, 国内会議

    ポスター発表

  • Assay of PCB congeners in transgenic arabidopsis plants with recombinant guinea pig AhR-mediated GUS reporter gene system

    Sayuri Shimazu, Masaya Ohta, Hideyuki Inui, Hitoshi Ashida, Hideo Ohkawa

    The International Chemical Congress of Pacific Basin Societies, 2010年12月, 英語, Honolulu; USA, 国際会議

    口頭発表(一般)

  • 残留性有機汚染物質の植物による吸収及び蓄積機構の解明

    乾秀之

    第11回IETセミナー, 2010年10月, 日本語, 東京, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Phytomonitoring of environmental contaminants with transgenic plants carrying recombinant mammalian receptors

    Hideyuki Inui

    Lab Seminar at Nam-Hai Chua Lab of the Rockefeller University, 2010年10月, 英語, New York; USA, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Comparison of the metabolic activities toward 3;3';4;4'-tetrachlorobiphenyl of rat and human CYP1A1s

    Miku Yabu, Kiyoshi Yamazaki, Motoharu Suzuki, Toshimasa Ito, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hideyuki Inui

    10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, 2010年10月, 英語, Woods Hole; USA, 国際会議

    ポスター発表

  • Clarification of metabolism of 3;3';4;4';5-pentachlorobiphenyl by rat CYP1A1 using the docking model

    Kiyoshi Yamazaki, Motoharu Suzuki, Toshimasa Ito, Keiko Yamamoto, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, Hiromasa Imaishi, Hideyuki Inui

    10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, 2010年10月, 英語, Woods Hole; USA, 国際会議

    ポスター発表

  • 抗体可変領域を用いた新規遺伝子発現調節システムの創製と応用

    乾秀之

    第1回フォローアップ勉強会, 2010年09月, 日本語, 大阪, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • シロイヌナズナ LOV KELCH PROTEIN2(LKP2);CONSTANS(CO)によるジャガイモ塊茎形成の制御

    西山雄樹, 乾秀之, 小倉康裕, 菊池彰, 中曽根光, 高瀬智敬, 清末知宏

    第28回日本植物細胞分子生物学会大会, 2010年09月, 日本語, 仙台, 国内会議

    口頭発表(一般)

  • イムノアフィニティーカラムを利用した水環境中有害化学物質の高感度分析

    乾秀之

    第13回日本水環境学会シンポジウム, 2010年09月, 日本語, 京都, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • どんな物質でも誘導剤にできる抗体可変領域を用いた遺伝子発現調節システム

    乾秀之

    第24回バイオテクノロジー産業化のための技術シーズ公開会, 2010年08月, 日本語, 大阪, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • ズッキーニ品種におけるダイオキシン類の蓄積性能の比較

    兒玉典子, 松尾申遼, 殷熙洙, 山崎清志, 乾秀之

    日本農薬学会第35回大会, 2010年05月, 日本語, 札幌, 国内会議

    口頭発表(一般)

  • 哺乳動物シトクロムP450による五塩素化ビフェニルの代謝

    山崎清志, 鈴木元治, 松村千里, 中野武, 榊利之, 今石浩正, 乾秀之

    2010年度日本農芸化学会, 2010年03月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • 抗体可変領域を用いた抗体転写因子の高感度化に影響を与えるドメイン配列

    河野実織, 櫻井由季, 祗園景子, 乾秀之

    生化学, 2010年, 日本語, 国内会議

    ポスター発表

  • Assay of PCB congeners in transgenic Arabidopsis plants with recombinant guinea pig AhR-mediated GUS reporter gene system

    SHIMAZU Sayuri, OHTA Masaya, INUI Hideyuki, ASHIDA Hitoshi, OHKAWA Hideo

    PACIFICHEM2010(環太平洋国際化学会議), 2010年, 英語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • どんな物質でも誘導剤にできる抗体可変領域を用いた遺伝子発現調節システム

    乾秀之

    第4回(大阪・兵庫・和歌山地区)非公開型科学技術情報交換会, 2009年12月, 日本語, 大阪, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 抗体可変領域を用いた転写因子によるポリ塩化ビフェニル測定法の高感度化

    櫻井由季, 祗園景子, 乾秀之

    第82回日本生化学会大会, 2009年10月, 日本語, 神戸, 国内会議

    口頭発表(一般)

  • 抗体可変領域を用いた人工転写因子の交叉反応性

    櫻井由季, 祇園景子, 山崎清志, 乾秀之

    第82回日本生化学会大会, 2009年10月, 日本語, 神戸, 国内会議

    ポスター発表

  • 抗体可変領域を用いた転写因子によるポリ塩化ビフェニル測定法の高感度化

    櫻井由季, 祗園景子, 乾秀之

    生化学, 2009年09月, 日本語, 国内会議

    ポスター発表

  • 抗体可変領域を用いた人工転写因子の交叉反応性

    櫻井由季, 祗園景子, 山崎清志, 乾秀之

    生化学, 2009年09月, 日本語, 国内会議

    ポスター発表

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収経路の解明

    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, 池田 健一, 朴 杓允, 今石 浩正, 乾 秀之

    第18回環境化学討論会, 2009年06月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収・蓄積性能

    乾秀之, 山崎清志, 岩田徹, 松尾申遼, 沢田真美, 祗園景子, 若井丈人, KIM Yun‐Seok, EUN Heesoo

    環境化学討論会講演要旨集, 2009年06月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収・蓄積性能

    乾秀之, 山崎清志, 岩田徹, 松尾申遼, 沢田真美, 祗園景子, 若井丈人, 金倫碩, 殷熙洙

    第18回環境化学討論会, 2009年06月, 日本語, つくば, 国内会議

    ポスター発表

  • How are recombinant P450s and AhRs useful for phytomonitoring and phytoremediation of environmental chemicals?

    Hideo Ohkawa, Hideyuki Inui

    16th International Conference on Cytochrome P450 Biochemistry; Biophysics; Biotechnology, 2009年06月, 英語, Nago; Okinawa, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Effects of biosurfactants on bioassay of dioxins in transgenic Arabidopsis plants with recombinant AhR/GUS reporter genes

    Sayuri Shimazu, Masaya Ohta, Hideyuki Inui, Hideo Ohkawa

    16th International Conference on Cytochrome P450 Biochemistry; Biophysics; Biotechnology, 2009年06月, 英語, Nago; Okinawa, 国際会議

    ポスター発表

  • A high-throughput bioassay for dioxins and dioxin-like compounds using transgenic Arabidopsis plants expressing a recombinant aryl hydrocarbon receptor gene

    Hideyuki Inui, Keiko Gion, Heesoo Eun, Hideo Ohkawa

    16th International Conference on Cytochrome P450 Biochemistry; Biophysics; Biotechnology, 2009年06月, 英語, Nago; Okinawa, 国際会議

    ポスター発表

  • 組換え型AhR/GUSレポーター遺伝子導入シロイヌナズナによるダイオキシン類アッセイにおけるバイオサーファクタントの効果

    嶋津小百合, 稲吉亮人, 太田雅也, 乾秀之, 大川秀郎

    2009年度日本農芸化学会, 2009年03月, 日本語, 福岡, 国内会議

    ポスター発表

  • ズッキーニ植物における残留性有機汚染物質の吸収・移行経路

    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, 池田 健一, 朴 杓允, 今石 浩正, 乾 秀之

    2009年度日本農芸化学会, 2009年03月, 日本語, 福岡, 国内会議

    口頭発表(一般)

  • ズッキーニにおけるダイオキシン類の品種特異的蓄積

    松尾申遼, 山崎清志, 祗園景子, 殷熙洙, 乾秀之

    日本農薬学会第34回大会, 2009年03月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収・移行・蓄積性能

    乾秀之, 若井丈人, 祗園景子, 金倫碩, 殷熈洙

    日本農芸化学会大会講演要旨集, 2009年03月, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収・移行・蓄積性能

    乾秀之, 若井丈人, 祗園景子, 金倫碩, 殷熙洙

    2009年度日本農芸化学会, 2009年03月, 日本語, 福岡, 国内会議

    ポスター発表

  • 植物で生産した組換え型アリルハイドロカーボン受容体を利用したダイオキシン類のin vitroバイオアッセイ系の開発

    辻林義寛, 祗園景子, 乾秀之

    日本農芸化学会関西支部第458回講演会, 2009年02月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • ダイオキシン類を吸収・蓄積するズッキーニの根滲出物による土壌からのダイオキシン類脱離・可溶化作用

    岩田徹, 山崎清志, 祗園景子, 若井丈人, 金倫碩, 殷熙洙, 乾秀之

    日本農芸化学会関西支部第458回講演会, 2009年02月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • ズッキーニにおけるダイオキシン類の品種特異的蓄積

    松尾申遼, 山崎清志, 祗園景子, EUN Heesoo, 乾秀之

    日本農薬学会大会講演要旨集, 2009年02月, 日本語, 国内会議

    口頭発表(一般)

  • 9A28 ズッキー二におけるダイオキシン類の品種特異的蓄積(残留・分析法,一般講演要旨)

    松尾 申遼, 山崎 清志, 砥園 景子, 殷 煕沫, 乾 秀之

    日本農薬学会, 2009年02月, 日本語, 国内会議

    口頭発表(一般)

  • 植物で生産した組換え型アリルハイドロカーボン受容体を利用したダイオキシン類のin vitroバイオアッセイ系の開発

    辻林義寛, 祇園景子, 乾秀之

    日本農芸化学会関西支部講演会講演要旨集, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • ダイオキシン類を吸収・蓄積するズッキーニの根滲出物により土壌からのダイオキシン類脱離・可溶化作用

    岩田徹, 山崎清志, 祇園景子, 若井丈人, 金倫碩, 殷煕洙, 乾秀之

    日本農芸化学会関西支部講演会講演要旨集, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • ズッキーニ植物における残留農薬有機汚染物質の吸収・移行経路

    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, 池田 健一, 朴 杓允, 今石 浩正, 乾 秀之

    日本農芸化学会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収経路の解明

    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, 池田 健一, 朴 杓允, 今石 浩正, 乾 秀之

    環境化学討論会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 動物特有の環境遺伝子を導入した遺伝子組換え植物の環境汚染モニタリングへの利用—ダイオキシン受容体を例に—

    乾秀之

    遺伝子若手シンポジウム, 2008年12月, 日本語, 神戸, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 抗体可変領域を用いた転写因子によるPCB測定法の開発

    櫻井由季, 祗園景子, 渡昭人, 乾秀之

    第31回日本分子生物学会年会第81回日本生化学会大会合同大会, 2008年12月, 日本語, 神戸, 国内会議

    ポスター発表

  • 抗体可変領域を用いた新規遺伝子発現調節システム

    祇園景子, 渡昭人, 櫻井由季, 乾秀之

    第31回日本分子生物学会年会第81回日本生化学会大会合同大会, 2008年12月, 日本語, 神戸, 国内会議

    ポスター発表

  • どんな物質でも誘導剤にできる遺伝子発現調節システム

    乾秀之, 祗園景子

    甲南大学・神戸大学 新技術説明会, 2008年10月, 日本語, 東京, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • P450によるPCB;ドリン;ヘプタクロルの分解

    乾秀之

    新農業展開ゲノムプロジェクトポスターセッション, 2008年10月, 日本語, 京都, 国内会議

    ポスター発表

  • アリルハイドロカーボン受容体を導入した形質転換植物によるダイオキシン類のバイオアッセイ

    祗園景子, 大川秀郎, 乾秀之

    環境化学討論会講演要旨集, 2008年06月, 日本語, 国内会議

    口頭発表(招待・特別)

  • アリルハイドロカーボン受容体を導入した形質転換植物によるダイオキシン類のバイオアッセイ

    祗園景子, 大川秀郎, 乾秀之

    第17回環境化学討論会, 2008年06月, 日本語, 神戸, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Metabolism of polychlorinated biphenyls by mammalian cytochrome P450 monooxygenases and its application to phytoremediation

    Hideyuki Inui, Miki Kanemitsu, Yasuo Fukami, Toshiyuki Sakaki, Keiko Gion, Kiyoshi Yamazaki, Hideo Ohkawa

    9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, 2008年06月, 英語, Nice; France, 国際会議

    ポスター発表

  • ecombinant P450s and AhRs: How they are improved in their fuctions

    Hideo Ohkawa, Hideyuki Inui

    9th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, 2008年06月, 英語, Nice; France, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 哺乳動物薬物代謝型シトクロムP450分子種によるコプラナーポリ塩化ビフェニルの代謝

    金光美希, 深見泰夫, 榊利之, 乾秀之

    日本農芸化学会2008年度大会, 2008年03月, 日本語, 名古屋, 国内会議

    口頭発表(一般)

  • 組換え型アリル炭化水素受容体と細胞間移行転写因子遺伝子を導入した形質転換植物によるダイオキシン類のバイオアッセイ

    祇園景子, 大川秀郎, 乾秀之

    日本農芸化学会大会講演要旨集, 2008年03月, 日本語, 国内会議

    口頭発表(一般)

  • 組換えアリルハイドロカーボン受容体と細胞間移行転写因子を用いた形質転換植物によるダイオキシン類のバイオアッセイ

    祗園景子, 大川秀郎, 乾秀之

    日本農芸化学会2008年度大会, 2008年03月, 日本語, 名古屋, 国内会議

    口頭発表(一般)

  • 植物と動物の特性を活かした環境汚染物質のバイオアッセイと浄化

    乾秀之

    兵庫県立健康環境科学研究センター学術交流会, 2008年03月, 日本語, 神戸, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 残留性有機汚染物質を効率よく蓄積するズッキーニにおけるダイオキシン類の吸収・移行メカニズム

    乾秀之, 若井丈人, 祇園景子, KIM Yun‐Seok, EUN Heesoo

    日本農薬学会大会講演要旨集, 2008年03月, 日本語, 国内会議

    口頭発表(一般)

  • 残留性有機汚染物質を効率よく蓄積するズッキーニにおけるダイオキシン類の吸収・移行メカニズム

    乾秀之, 若井丈人, 祗園景子, 金倫碩, 殷熙洙

    日本農薬学会第33回大会, 2008年03月, 日本語, 奈良, 国内会議

    口頭発表(一般)

  • 残留性有機汚染物質の吸収に差があるズッキーニ品種間において発現量が異なる遺伝子の探索

    乾秀之, 田中マチ子, 祇園景子

    日本農芸化学会大会講演要旨集, 2008年03月, 日本語, 国内会議

    口頭発表(一般)

  • 残留性有機汚染物質の吸収に差があるズッキーニ品種間において発現量が異なる遺伝子の探索

    乾秀之, 田中マチ子, 祗園景子

    日本農芸化学会2008年度大会, 2008年03月, 日本語, 名古屋, 国内会議

    口頭発表(一般)

  • 抗体の可変領域断片を用いた新規遺伝子発現調節システムの構築

    渡昭人, 祗園景子, 乾秀之

    日本農芸化学会関西支部第453回講演会, 2008年03月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • 抗体可変領域を用いた新規遺伝子発現調節システム

    祗園景子, 渡昭人, 櫻井由季, 乾秀之

    生化学, 2008年, 日本語, 国内会議

    ポスター発表

  • 抗体の可変領域断片を用いた新規遺伝子発現調節システムの構築

    渡昭人, 祇園景子, 乾秀之

    日本農芸化学会関西支部講演会講演要旨集, 2008年, 日本語, 国内会議

    口頭発表(一般)

  • 驚異の植物、ズッキーニ!

    乾 秀之

    産学パートナーズシップ事業, 2007年05月, 日本語, 姫路, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 驚異の植物;ズッキーニ!

    乾 秀之

    産学パートナーズシップ事業, 2007年05月, 日本語, 姫路, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 残留性有機汚染物質を効率よく吸収するズッキーニにおけるダイオキシン類の吸収・蓄積

    乾 秀之, 若井 丈人, 祗園 景子, 金 倫碩, 殷 熙洙

    日本農薬学会第32回大会, 2007年04月, 日本語, 日本農薬学会, 東京, 国内会議

    口頭発表(一般)

  • 水生植物ヒルムシロ属における高温ストレス順応性の比較

    天野百々江, 飯田聡子, 岩崎哲史, 深見泰夫, 乾秀之, 小菅桂子

    第54回日本生態学会大会, 2007年03月, 日本語, 松山, 国内会議

    ポスター発表

  • 残留性有機汚染物質を効率よく吸収するズッキーニにおけるダイオキシン類の吸収・蓄積

    乾秀之, 若井丈人, 祇園景子, KIM Yun‐Seok, EUN Heesoo

    日本農薬学会大会講演要旨集, 2007年03月, 日本語, 国内会議

    口頭発表(一般)

  • 残留性有機汚染物質を効率よく吸収するズッキーニにおけるダイオキシン類の吸収・蓄積

    乾 秀之, 若井 丈人, 祇園 景子, 金 倫碩, 殷 熙洙

    講演要旨集, 2007年03月, 日本語, 国内会議

    口頭発表(一般)

  • マウス及びモルモットのアリルハイドロカーボン受容体遺伝子系を導入した形質転換植物によるダイオキシン類のモニタリング

    乾 秀之, 祗園 景子, 烏谷 泰, 殷 熙洙, 服部 眞幸, 荒尾 知人, 遠藤 正造, 大川 秀郎

    2007年度日本農芸化学会, 2007年03月, 日本語, 日本農芸化学会, 東京, 国内会議

    口頭発表(一般)

  • マウス及びモルモットのアリルハイドロカーボン受容体/レポーター遺伝子系を導入した形質転換植物による残留性有機汚染物質のバイオアッセイ

    祇園景子, 若井丈人, 金倫碩, 殷熙洙, 乾秀之, 大川秀郎

    2007年度日本農芸化学会, 2007年03月, 日本語, 日本農芸化学会, 東京, 国内会議

    口頭発表(一般)

  • マウス及びモルモットのアリルハイドロカーボン受容体

    祇園景子, 若井丈人, 金倫碩, 殷煕洙, 乾秀之, 大川秀郎

    日本農芸化学会大会講演要旨集, 2007年03月, 日本語, 国内会議

    口頭発表(一般)

  • ズッキーニ品種における残留性有機汚染物質の効率的な吸収に関する遺伝子の探索

    乾 秀之, 若井 丈人, 祗園 景子, 金 倫碩, 殷 熙洙

    2007年度日本農芸化学会, 2007年03月, 日本語, 日本農芸化学会, 東京, 国内会議

    口頭発表(一般)

  • ズッキーニにおける残留性有機汚染物質の高吸収に関する要因の探索

    若井丈人, 祇園景子, 金倫碩, 殷煕沫, 乾秀之

    日本農芸化学会大会講演要旨集, 2007年03月, 日本語, 国内会議

    口頭発表(一般)

  • ズッキーニにおける残留性有機汚染物質の高吸収に関する要因の探索

    若井 丈人, 祗園 景子, 金 倫碩, 殷 熙洙, 乾 秀之

    2007年度日本農芸化学会, 2007年03月, 日本語, 日本農芸化学会, 東京, 国内会議

    口頭発表(一般)

  • ズッキーニにおける残留性有機汚染物質の効率的な吸収に関する遺伝子の探索

    乾秀之, 若井丈人, 祇園景子, 金倫碩, 殷煕沫

    日本農芸化学会大会講演要旨集, 2007年03月, 日本語, 国内会議

    口頭発表(一般)

  • Remediation of simazine and 4-nonylphenol with transgenic plants carrying drug-metabolizing cytochrome P450 genes

    INUI Hideyuki, OKA Natsuyo, OHKAWA Hideo

    11th IUPAC International Congress of Pesticide Chemistry, 2006年08月, 英語, Kobe, 国際会議

    ポスター発表

  • Phytoremediation of pollutants

    INUI Hideyuki

    11th IUPAC International Congress of Pesticide Chemistry, 2006年08月, 英語, Kobe, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Monitoring of estrogenic compounds in transgenic plants carrying estrogen receptor and reporter genes

    INUI Hideyuki

    11th IUPAC International Congress of Pesticide Chemistry, 2006年08月, 英語, Kobe, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Engineering of the transgenic plants carrying the receptor-mediated reporter gene expression systems for bioassay of POPs

    GION Keiko, INUI Hideyuki, KODAMA Susumu, SASAKI Hideaki, UTANI Yasushi, EUN Heesoo, HATTORI Masayuki, ARAO Tomohito, ENDO Shozo, OHKAWA Hideo

    11th IUPAC International Congress of Pesticide Chemistry, 2006年08月, 英語, Kobe, 国際会議

    ポスター発表

  • Bioassay of persistent organic pollutants in transgenic plants with Ah receptor and GUS reporter genes

    INUI Hideyuki, GION Keiko, UTANI Yasushi, OHKAWA Hideo

    11th IUPAC International Congress of Pesticide Chemistry, 2006年08月, 英語, Kobe, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Bioassays for persistent organic pollutants (POPs) in transgenic plants carrying AhR and GUS reporter genes

    OHKAWA Hideo, INUI Hideyuki, TANAKA Yoshikazu

    8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, 2006年07月, 英語, Swansea, UK, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 植物と動物の特性を生かした環境汚染物質のバイオアッセイと浄化

    INUI Hideyuki

    CASTクラブ第20回例会講演会, 2006年06月, 日本語, 播磨, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 哺乳動物由来の受容体を介したレポーター遺伝子発現系を用いた残留性有機汚染物質のファイトモニタリング

    乾秀之, 祇園景子, 大川秀郎

    日本農薬学会大会講演要旨集, 2006年03月, 日本語, 国内会議

    口頭発表(一般)

  • 哺乳動物薬物代謝酵素シトクロムP450の遺伝子を付与・発現した形質転換植物による環境負荷化学物質のファイトレメディエーション

    岡 夏世, 乾 秀之, 大野 清春, 大川 秀郎

    日本農薬学会第31回大会, 2006年03月, 日本語, 日本農薬学会, 福岡, 国内会議

    口頭発表(一般)

  • 有機リン系殺虫剤フェニトロチオンに対するモノクローナル抗体を用いたイムノアフィニティーカラムの開発

    上杉 明里, 乾 秀之, 土居 史人, 佐々木 満, 大野 清春, 大川 秀郎

    日本農薬学会第31回大会, 2006年03月, 日本語, 福岡, 国内会議

    口頭発表(一般)

  • 組換え型AhR

    川畑順子, 祇園景子, 児玉進, 乾秀之, 大川秀郎

    日本農芸化学会大会講演要旨集, 2006年03月, 日本語, 国内会議

    口頭発表(一般)

  • マウス及びモルモットのアリルハイドロカーボン受容体遺伝子系を導入した形質転換植物によるダイオキシン類のモニタリング

    乾秀之, 祇園景子, 烏谷泰, 殷煕洙, 服部眞幸, 荒尾和人, 遠藤正造, 大川秀郎

    日本農芸化学会大会講演要旨集, 2006年03月, 日本語, 国内会議

    口頭発表(一般)

  • マウス・アリルハイドロカーボン受容体とβ-グルクロニダーゼレポーターの遺伝子系を導入した形質転換タバコにおけるダイオキシン類のモニタリング

    烏谷 泰, 祇園 景子, 乾 秀之, 殷 熙洙, 服部 眞幸, 荒尾 知人, 遠藤 正造, 大川 秀郎

    2005年度日本農芸化学会, 2006年03月, 日本語, 日本農芸化学会, 札幌, 国内会議

    ポスター発表

  • ビスフェノールAに特異的な単鎖可変領域抗体を用いたイムノアフィニティカラムの開発

    土居 史人, 武内 哲也, 乾 秀之, 三宅 司郎, 大川 秀郎

    2005年度日本農芸化学会, 2006年03月, 日本語, 日本農芸化学会, 札幌, 国内会議

    ポスター発表

  • ダイオキシン類に高い感受性を示すモルモットのアリルハイドロカーボン受容体遺伝子系を導入した形質転換植物の作出

    祇園 景子, 佐々木 秀明, 鳥谷 泰, 乾 秀之, 大川 秀郎

    2005年度日本農芸化学会, 2006年03月, 日本語, 日本農芸化学会, 札幌, 国内会議

    ポスター発表

  • Monitoring of nano-level concentrations of persistent organic pollutants(POPs) in transgenic plants

    OHKAWA Hideo, INUI Hideyuki, TANAKA Yoshikazu

    231th ACS National Meeting, 2006年03月, 英語, Atlanta, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 異物代謝酵素系を導入した形質転換植物による環境負荷化学物質の負荷軽減技術の開発

    乾 秀之

    食と環境の安全を求めて:有害化学物質のリスク評価と低減技術, 2005年11月, 日本語, (独)農業環境技術研究所, つくば, 国内会議

    ポスター発表

  • 哺乳動物受容体遺伝子系を導入した形質転換植物を利用した環境負荷化学物質のモニタリング法の開発

    乾 秀之

    岡山大学・学内COEキックオフシンポジウム、「資源生物を用いた地球環境のモニター系の構築と環境保全への応用」, 2005年10月, 日本語, 岡山大学, 岡山, 国内会議

    口頭発表(招待・特別)

  • 哺乳動物受容体遺伝子系を導入した形質転換植物を利用した環境負荷化学物質のモニタリング法の開発

    乾 秀之

    岡山大学・学内COEキックオフシンポジウム;「資源生物を用いた地球環境のモニター系の構築と環境保全への応用」, 2005年10月, 日本語, 岡山大学, 岡山, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Monitoring of estrogenic chemicals in transgenic plants carrying ER and GFP genes

    INUI Hideyuki

    Frontiers in Environmental Sciences, 2005年10月, 英語, 京都大学, Kyoto, 国際会議

    口頭発表(招待・特別)

  • マウス・アリルハイドロカーボン受容体とβ‐グルクロニダーゼレポーターの遺伝子系を導入した形質転換タバコにおけるダイオキシン類のモニタリング

    烏谷泰, 祇園景子, 乾秀之, いん きしゅ, 服部真幸, 荒尾知人, 遠藤正造, 大川秀郎

    日本農芸化学会大会講演要旨集, 2005年03月, 日本語, 国内会議

    口頭発表(一般)

  • ダイオキシン類に高い感受性を示すモルモットのアリルハイドロカーボン受容体遺伝子系グ導入した形質転換植物の作出

    祇園景子, 佐々木秀明, 烏谷泰, 乾秀之, 大川秀郎

    日本農芸化学会大会講演要旨集, 2005年03月, 日本語, 国内会議

    口頭発表(一般)

  • エストロジェン受容体遺伝子系を付与・発現した形質転換シロイヌナズナおける内分泌攪乱化学物質のモニタリング

    乾 秀之, 下村 直史, 佐々木 秀明, CHUA Nam-Hai, 大川 秀郎

    2005年度日本農芸化学会, 2005年03月, 日本語, 日本農芸化学会, 札幌, 国内会議

    ポスター発表

  • エストロジェン受容体遺伝子系を付与・発現した形質転換シロイヌナズナおける内分泌攪乱化学物質のモニタリング

    乾 秀之, 下村 直史, 佐々木 秀明, CHUA Nam-Hai, 大川 秀郎

    2005年度日本農芸化学会, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議

    ポスター発表

  • 脂肪酸水酸化反応を触媒するトウモロコシCYP78A1を発現させた組替え体シロイヌナズナの解析

    藤本 健司, 今石 浩正, 佐々木 満, 滝川 浩郷, 乾 秀之, 大野 清春

    日本農芸化学会関西支部第437回講演会, 2004年12月, 日本語, 日本農芸化学会関西支部, 神戸大学, 国内会議

    口頭発表(一般)

  • メダカ・エストロジェン受容体を付与・発現したシロイヌナズナにおける内分泌攪乱化学物質のモニタリング

    下村 直史, 乾 秀之, 大川 秀郎

    日本内分泌攪乱化学物質学会 第7回研究発表会, 2004年12月, 日本語, 日本内分泌攪乱化学物質学会, 名古屋, 国内会議

    ポスター発表

  • ヒト・エストロジェン受容体遺伝子系を付与・発現した形質転換シロイヌナズナにおける内分泌攪乱化学物質のモニタリング

    乾 秀之, 佐々木 秀明, CHUA Nam-Hai, 大川 秀郎

    日本内分泌攪乱化学物質学会 第7回研究発表会, 2004年12月, 日本語, 日本内分泌攪乱化学物質学会, 名古屋, 国内会議

    ポスター発表

  • Monitoring of dioxines and endocrine disruptors in transgenic plants carrying aryl hydrocarbon receptor and estrogen receptor genes

    INUI Hideyuki, SASAKI Hideaki, KODAMA Susumu, CHUA Nam-Hai, OHKAWA Hideo

    7th International Symposium on Cytochrome P450 Biodiversity amd Biotechnology, 2004年08月, 英語, Cytochrome P450 Biodiversity and Biotechnology Organizing Committee, Awaji, 国際会議

    口頭発表(招待・特別)

  • Molecular mechanism of induction of the tobaccp CYP71A11 gene by treatment with the herbicide bromoxynil

    INUI Hideyuki, TAKAKUMA Kazuyuki, OHKAWA Hideo

    7th International Symposium on Cytochrome P450 Biodiversity amd Biotechnology, 2004年08月, 英語, Cytochrome P450 Biodiversity and Biotechnology Organizing Committee, Awaji, 国際会議

    ポスター発表

  • Engineering of transgenic tobacco plants carrying a recombinant guinea pig aryl hydrocarbon receptor gene for monitoring of dioxins

    GION Keiko, SASAKI Hideaki, INUI Hideyuki, UTANI Yasushi, KODAMA Susumu, OKADA Kumiko, OHKAWA Hideo

    7th International Symposium on Cytochrome P450 Biodiversity amd Biotechnology, 2004年08月, 英語, Cytochrome P450 Biodiversity and Biotechnology Organizing Committee, Awaji, 国際会議

    ポスター発表

  • Aiming for ornamental plants monitoring environmental pollutants

    TANAKA Yoshikazu, TOGAMI Junichi, OKUHARA Hiroaki, TAMURA Mihoko, ISHIGURO Kanako, HIROSE Chika, INUI Hideyuki, OHKAWA Hideo

    7th International Symposium on Cytochrome P450 Biodiversity amd Biotechnology, 2004年08月, 英語, Cytochrome P450 Biodiversity and Biotechnology Organizing Committee, Awaji, 国際会議

    口頭発表(招待・特別)

  • エストロジェン受容体遺伝子系を付与・発現した環境負荷化学物質モニタリング用植物の開発

    乾 秀之

    日本農芸化学会学会創立80周年記念シンポジウム「明日の農芸化学を担う若い力」, 2004年07月, 日本語, 日本農芸化学会, 大阪, 国内会議

    口頭発表(招待・特別)

  • コプラナーPCBの免疫化学測定法

    武内 哲也, 西 甲介, 高井 幹夫, 乾 秀之, 三宅 司郎, 今井 哲弥, 大川 秀郎

    免疫化学測定法研究会 第9回学術集会, 2004年06月, 日本語, 免疫化学測定法研究会, 東京, 国内会議

    口頭発表(一般)

  • 除草剤シマジンに特異的なモノクローナル抗体及び組換え抗体scFvを用いた免疫化学測定法

    藤井 律子, 西 甲介, 乾 秀之, 今井 哲弥, 大川 秀郎

    日本農薬学会第29回大会, 2004年03月, 日本語, 日本農薬学会, 神戸, 国内会議

    口頭発表(一般)

  • 除草剤アラクロールに特異的なモノクローナル抗体を用いた免疫化学測定法による環境水飼料の測定

    松尾 良子, 土居 史人, 森宗 孝介, 西 甲介, 乾 秀之, 大川 秀郎

    日本農薬学会第29回大会, 2004年03月, 日本語, 日本農薬学会, 神戸, 国内会議

    口頭発表(一般)

  • 殺虫剤マラチオンに特異的抗体を用いたイムノアフィニティーカラムの開発

    竹原 明良, 西 甲介, 三宅 司郎, 乾 秀之, 大川 秀郎

    日本農薬学会第29回大会, 2004年03月, 日本語, 日本農薬学会, 神戸, 国内会議

    口頭発表(一般)

  • モルモット・アリルハイドロカーボン受容体遺伝子を導入した形質転換タバコにおけるダイオキシン類のモニタリング

    佐々木 秀明, 乾 秀之, 烏谷 泰, 児玉 進, 大川 秀郎

    日本農芸化学会2004年度大会, 2004年03月, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • Herbicide resistance in transgenic plants with mammalian P450 monooxydenases

    INUI Hideyuki, OHKAWA Hideo

    227th ACS National Meeting, 2004年03月, 英語, American Chemical Society, Anaheim, USA, 国際会議

    口頭発表(招待・特別)

  • ヒト・エストロジェン受容体遺伝子系を付与・発現した形質転換シロイヌナズナにおける内分泌攪乱化学物質のモニタリング

    乾 秀之, 佐々木 秀明, Nam-Hai Chua, 大川 秀郎

    日本内分泌攪乱化学物質学会 第7回研究発表会, 2004年, 日本語, 日本内分泌攪乱化学物質学会, 名古屋, 国内会議

    口頭発表(一般)

  • Monitoring of endocrine disruptors in transgenic plants carrying aryl hydrocarbon receptor and estrogen receptor genes

    INUI Hideyuki, SASAKI Hideaki, KODAMA Susumu, CHUA Nam-Hai, OHKAWA Hideo

    3rd Pan-Pacific Conference on Pesticide Science, 2003年06月, 英語, Pesticide Science Society of Japan, Hawaii, USA, 国際会議

    口頭発表(招待・特別)

  • Monitoring of endocrine disruptors in transgenic plants carrying aryl hydrocaebon receptor and estrogen receptor genes

    INUI Hideyuki, SASAKI H, KODAMA S, CHUA N.-H, OHKAWA Hideo

    3rd Pan-Pacific Conference on Cytochrome P450 Biochemistry, 2003年06月, 英語, 未記入, Hilton, Hawaii, 国際会議

    口頭発表(一般)

  • エストロジェンレセプター系を導入した内分泌攪乱化学物質のモニタリング用シロイヌナズナの作出

    乾秀之, 佐々木秀明, 児玉進, Nam-Hai Chua, 大川秀郎

    2003年度日本農芸化学会大会, 2003年03月, 日本語, 藤沢, 国内会議

    口頭発表(一般)

  • エストロジェンレセプター系を用いた内分泌攪乱化学物質のモニタリング用植物

    乾秀之, 児玉進, Nam-Hai Chua, 大川秀郎

    第75回日本生化学会, 2002年10月, 日本語, 京都, 国内会議

    ポスター発表

  • Strategies for Transgenic Plants Monitoring and Reducing Pesticide Residues

    大川 秀郎, 乾 秀之, 今石 浩正

    Sixth International Symposium on Cytochrome P450 Biodiversity, 2002年08月, 英語, Los Angeles,USA., 国際会議

    口頭発表(一般)

  • Molecular Function of Cytochrome P450 Species as Targets for Pest Management Chemicals

    大川 秀郎, 乾 秀之, 今石 浩正

    10th IUPAC International Congress on the Chemistry of Crop Protection, 2002年08月, 英語, Basel,Switzerland, 国際会議

    口頭発表(一般)

  • Herbicide metabolism and tolerance in the transgenic plants expressing human CYP2C9 and CYP2C19

    Hideyuki Inui, Noriaki Shiota, Yoshiko Ido, Tomomi Inoue, Sakiko Hirose, Hiroyuki Kawahigashi, Yasunobu Ohkawa, Hideo Ohkawa

    10th IUPAC International Congress on the Chemistry of Crop Protection, 2002年08月, 英語, Basel; Switzerland, 国際会議

    口頭発表(一般)

  • タバコCYP71A11遺伝子の化学物質誘導型プロモーターの機能解析

    祇園景子, 乾秀之, 大川秀郎

    生化学, 2001年08月, 日本語, 国内会議

    ポスター発表

  • タバコ植物におけるCYP71A11遺伝子プロモーターを用いたヒトCYP2C19の化学物質誘導発現と除草剤代謝・耐性

    中井秀一, 乾秀之, 祇園景子, 大川秀郎

    日本農芸化学会誌, 2001年04月, 日本語, 国内会議

    口頭発表(一般)

  • 化学物質誘導型タバコCYP71A11プロモーターの機能解析

    祇園景子, 乾秀之, 中井秀一, 大川秀郎

    日本農芸化学会誌, 2001年03月, 日本語, 国内会議

    口頭発表(一般)

  • タバコ植物におけるCYP71A11遺伝子プロモーターを用いたヒトCYP2C19の化学物質誘導発現と除草剤代謝・耐性

    中井秀一, 乾秀之, 祇園景子, 大川秀郎

    日本農芸化学会関西支部講演会講演要旨集, 2000年12月, 日本語, 国内会議

    口頭発表(一般)

共同研究・競争的資金等の研究課題

産業財産権

  • 抗体の可変領域を用いた新規な遺伝子発現調節方法

    乾 秀之, 祗園 景子

    特願2008-310132, 2008年12月04日, 大学長, 特許5858393, 2015年12月25日

    特許権

  • 植物の塊茎形成を制御するための塊茎形成制御ベクター、塊茎形成が制御された植物の製造方法および植物

    乾 秀之

    特願2007-054588, 2007年03月05日, 大学長, 特許5228169, 2013年03月29日

    特許権