研究者紹介システム
| 畠中 知子 |  |
| ハタナカ トモコ | |
| 大学院農学研究科 資源生命科学専攻 | |
| 准教授 | |
| 農学関係 |
研究者情報
所属
【主配置】
大学院農学研究科 資源生命科学専攻【配置】
農学部 資源生命科学科
学位
授業科目
- 特定課題演習 I-1, 大学院農学研究科, 2021
- 課題開発演習, 大学院農学研究科, 2021
- 資源生命科学プレゼンテーション演習Ⅰ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-1, 大学院農学研究科, 2021
- 特定課題演習 I-2, 大学院農学研究科, 2021
- 資源生命科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 資源植物生理生化学, 大学院農学研究科, 2021
- 資源植物開発論, 大学院農学研究科, 2021
- 高度教養セミナー農学部応用植物学入門, 農学部, 2021
- 高度教養セミナー農学部応用植物学入門, 農学部, 2021
- 高度教養セミナー農学部応用植物学, 農学部, 2021
- 卒業研究, 農学部, 2021
- 資源生命科学入門Ⅱ, 農学部, 2021
- 資源生命科学入門Ⅱ－１, 農学部, 2021
- 応用植物学専門実験 I, 農学部, 2021
- 食用作物学１, 農学部, 2021
- 食用資源植物学１, 農学部, 2021
- 食用作物学, 農学部, 2021
- 初年次セミナー（応用植物学コース）, 農学部, 2021
- 資源生命科学入門Ⅱ－２, 農学部, 2021
- 食用作物学２, 農学部, 2021
- 食用資源植物学２, 農学部, 2021
- 緑の保全, 農学部, 2021
- 緑の保全の探究, 農学部, 2021
- 植物資源学, 農学部, 2021
- 応用植物学基礎実験, 農学部, 2021
- 応用植物学専門実験 II, 農学部, 2021
- 産業資源植物学１, 農学部, 2021
- 産業資源植物学, 農学部, 2021
- 産業資源植物学２, 農学部, 2021
ジャンル
コメントテーマ
研究活動
研究分野
委員歴
- 2020年04月 - 現在, 近畿作物・育種研究会, 会計幹事
- 2018年10月 - 現在, 日本植物脂質研究会, 幹事
- 2016年04月 - 現在, 近畿作物・育種研究会, 評議員
- 2012年04月 - 2014年, 近畿作物・育種研究会, 会計幹事
受賞
論文
- Informa UK Limited, 2023年01月01日, Plant Production Science, 1 - 11
[査読有り]
研究論文（学術雑誌）
- Informa UK Limited, 2022年07月03日, Plant Production Science, 25 (3), 350 - 358
研究論文（学術雑誌）
Abstract Triacylglycerols (TAGs) are the major component of plant storage lipids such as oils. Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the Kennedy pathway, and is mainly responsible for plant oil accumulation. We previously found that the activity of Vernonia DGAT1 was distinctively higher than that of Arabidopsis and soybean DGAT1 in a yeast microsome assay. In this study, the DGAT1 cDNAs of Arabidopsis, Vernonia, soybean, and castor bean were introduced into Arabidopsis. All Vernonia DGAT1-expressing lines showed a significantly higher oil content (49% mean increase compared with the wild-type) followed by soybean and castor bean. Most Arabidopsis DGAT1-overexpressing lines did not show a significant increase. In addition to these four DGAT1 genes, sunflower, Jatropha, and sesame DGAT1 genes were introduced into a TAG biosynthesis-defective yeast mutant. In the yeast expression culture, DGAT1s from Arabidopsis, castor bean, and soybean only slightly increased the TAG content; however, DGAT1s from Vernonia, sunflower, Jatropha, and sesame increased TAG content >10-fold more than the former three DGAT1s. Three amino acid residues were characteristically common in the latter four DGAT1s. Using soybean DGAT1, these amino acid substitutions were created by site-directed mutagenesis and substantially increased the TAG content.
Oxford University Press (OUP), 2022年05月13日, Journal of Experimental Botany, 73 (9), 3030 - 3043, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
CO2-responsive CCT protein (CRCT) is a positive regulator of starch synthesis-related genes such as ADP-glucose pyrophosphorylase large subunit 1 and starch branching enzyme I particularly in the leaf sheath of rice (Oryza sativa L.). The promoter GUS analysis revealed that CRCT expressed exclusively in the vascular bundle, whereas starch synthesis-related genes were expressed in different sites such as mesophyll cell and starch storage parenchyma cell. However, the chromatin immunoprecipitation (ChIP) using a FLAG-CRCT overexpression line and subsequent qPCR analyses showed that the 5 '-flanking regions of these starch synthesis-related genes tended to be enriched by ChIP, suggesting that CRCT can bind to the promoter regions of these genes. The monomer of CRCT is 34.2 kDa; however, CRCT was detected at 270 kDa via gel filtration chromatography, suggesting that CRCT forms a complex in vivo. Immunoprecipitation and subsequent MS analysis pulled down several 14-3-3-like proteins. A yeast two-hybrid analysis and bimolecular fluorescence complementation assays confirmed the interaction between CRCT and 14-3-3-like proteins. Although there is an inconsistency in the place of expression, this study provides important findings regarding the molecular function of CRCT to control the expression of key starch synthesis-related genes.
WILEY, 2021年08月, Plant Cell and Environment, 44 (8), 2480 - 2493, 英語[査読有り]
研究論文（学術雑誌）
We screened 80 Asian rice (Oryza sativa L.) cultivars for the presence of the submergence-tolerance gene SUB1A-1 and the floating genes SNORKEL1 (SK1) and SNORKEL2 (SK2), and found that the deepwater rice cultivar Baisbish (BSB) and the submergence-tolerant cultivar Flood Resistant 13A (FR13A) both possess the SUB1A-1 and the SK1/2. When BSB and FR13A seedlings were completely submerged, spindly growth of shoots was induced in BSB but not in FR13A. Submergence significantly increased the SUB1A-1 transcript abundance in BSB and FR13A shoots, but the expression level in BSB was much lower than that of FR13A. Submergence also induced the expression of both ERF66 and ERF67, the transcriptional targets of SUB1A-1, in FR13A shoots, whereas it upregulated the expression of ERF67 but not that of ERF66 in BSB shoots. These results suggest that BSB could not display submergence tolerance due to the low expression of SUB1A-1 and/or ERF66 under submergence.
TAYLOR & FRANCIS LTD, 2021年07月, Plant Production Science, 英語[査読有り]
研究論文（学術雑誌）
- Informa UK Limited, 2021年04月15日, Soil Science and Plant Nutrition, 67 (3), 269 - 276
[査読有り]
研究論文（学術雑誌）
- 2020年, Journal of Crop Research, 65, 37 - 41, 英語Responses to complete submergence in African rice (Oryza glaberrima）
[査読有り][招待有り]
研究論文（学術雑誌）
- Informa UK Limited, 2019年, Plant Production Science, 22 (2), 296 - 300, 英語
[査読有り]
研究論文（学術雑誌）
The relationship between ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase (Rca) levels was studied using transgenic rice overexpressing maize Rca (OX-mRca) and knockdown transgenic rice expressing antisense Rca (KD-Rca). The ratio of Rubisco to total soluble protein was lower in OX-mRca, whereas it was higher in KD-Rca than in WT, indicating that Rca expression was negatively correlated with Rubisco content. The expressions of other Calvin–Benson–Bassham cycle enzymes such as sedoheptulose-1,7-bisphosphatase and phosphoribulokinase analyzed by immunoblotting did not show such a negative correlation with Rca, suggesting that the effect of Rca on protein expression may be specific for Rubisco. Although Rubisco content was decreased in OX-mRca, the transcript levels of the Rubisco large subunit (OsRbcL) and the Rubisco small subunit mostly increased in OX-mRca as well as in KD-Rca. Additionally, polysome loading of OsRbcL was slightly higher in OX-mRca than it was in WT, suggesting that the OsRbcL translation activity was likely stimulated by overexpression of Rca. 35S-methionine labeling experiments demonstrated that there was no significant difference in the stability of newly synthesized Rubisco among genotypes. However, 35S-methionine-labeled Rubisco was marginally decreased in OX-mRca and increased in KD-Rca compared to the WT. These results suggest that Rca negatively affects the Rubisco content, possibly in the synthesis step.
Springer Netherlands, 2018年05月30日, Photosynthesis Research, 1 - 10, 英語[査読有り]
研究論文（学術雑誌）
- Springer Science and Business Media LLC, 2018年02月, Plant Growth Regulation, 85 (1), 123 - 132, 英語
[査読有り]
研究論文（学術雑誌）
- 日本作物学会, 2017年, 日本作物学会講演会要旨集, 244, 124 - 124, 日本語
- 日本作物学会, 2017年, 日本作物学会講演会要旨集, 244, 123 - 123, 日本語
- 日本作物学会, 2017年, 日本作物学会講演会要旨集, 244, 119 - 119, 日本語
- 日本作物学会, 2017年, 日本作物学会講演会要旨集, 244, 122 - 122, 日本語
Rubisco is the key enzyme of photosynthetic CO2 fixation, whereas a Rubisco small subunit homolog (OsRbcS1) in rice, was exclusively expressed in non-photosynthetic organs. In this study, OsRbcS1-like genes were identified in several plant species including foxtail millet (SiRbcS1), tomato (SlRbcS1), Lotus japonicus (LjRbcS1), grape (VvRbcS1A, VvRbcS1B) and Selaginella moellendorffii (SmRbcS1A, SmRbcS1B). RT-PCR analysis revealed that SiRbcS1 was expressed in seeds of foxtail millet. In tomato, SlRbcS1 was expressed in the stamen, pistil and green fruit. In L. japonicus, LjRbcS1 was expressed in the root, nodule and seed. In grape, both VvRbcS1A and VvRbcS1B were expressed in mature leaves and the green berry. In S. moellendorffii, SmRbcS1A and SmRbcS1B displayed similar expression patterns and were expressed in the rhizome and root. Although these OsRbcS1-like genes were expressed in photosynthetic organs in S. moellendorffii, its expression levels were higher in non-photosynthetic organs than in photosynthetic organs. These results suggest that OsRbcS1-like RbcS functions in metabolic pathways other than photosynthetic CO2 fixation in various organs.
Elsevier, 2016年12月01日, Plant Gene, 8, 26 - 31, 英語[査読有り]
研究論文（学術雑誌）
- American Chemical Society (ACS), 2016年09月20日, Journal of Agricultural and Food Chemistry, 64 (38), 7188 - 7194
[査読有り]
研究論文（学術雑誌）
- Oxford University Press (OUP), 2016年08月12日, Plant and Cell Physiology, 57 (11), 2334 - 2341
[査読有り]
研究論文（学術雑誌）
- 日本作物学会, 2016年, 日本作物学会講演会要旨集, 241, 224 - 224, 日本語
- 日本作物学会, 2016年, 日本作物学会講演会要旨集, 241, 126 - 126, 日本語
- 日本作物学会, 2016年, 日本作物学会講演会要旨集, 241, 214 - 214, 日本語
- 日本作物学会, 2016年, 日本作物学会講演会要旨集, 241, 193 - 193, 日本語
- 日本作物学会, 2016年, 日本作物学会講演会要旨集, 241, 195 - 195, 日本語
ダイズの組織培養による再分化・植物体再生は，未熟子葉から体細胞胚を誘導し，増殖・形態分化・成熟の各ステージを経て，植物体を再生させる方法が主流である．このプロトコールはモデル品種であるJackでほぼ確立されているが，最適な培養方法は現在でも遺伝子型の違いに大きく左右される．代表的な日本産黒ダイズである丹波黒ではまだ正式な成功例が報告されていないが，我々の研究室では，Jackのプロトコールを改変することにより，丹波黒ダイズから体細胞胚を誘導・増殖し，効率は低いものの植物体再生にも成功してきた．これらの実験過程で，成熟培養だけでなく増殖培養でも培地に加える糖をスクロースからマルトースに置き換えることで，明らかな再分化促進効果を表すことがわかってきた．通常，増殖培養を2年以上も続けると，モデル品種であるJackでもその後の体細胞胚成熟効率が下がり，次第に再
近畿作物・育種研究会, 2015年11月, 作物研究, 60, 47 - 53, 日本語[査読有り]
研究論文（学術雑誌）
- 2015年02月, Plant Physiology, 167 (4), 1321 - 1331, 英語CO2-responsive CONSTANS, CONSTANS-like, and time of chlorophyll ab binding protein Expression1 protein is a positive regulator of starch synthesis in vegetative organs of rice.
[査読有り]
研究論文（学術雑誌）
- 日本作物学会, 2015年, 日本作物学会講演会要旨集, 240, 39 - 39, 日本語
Effects of overexpression of high activity-type Rubisco small subunit (RbcS) from a cold-resistant plant, timothy (Phleum pratense), on kinetic properties of Rubisco were studied in rice (Oryza sativa). The full-length mRNA sequence of timothy RbcS (PpRbcS1) was determined by 5'RACE and 3'RACE. The coding sequence of PpRbcS1 was fused to the chlorophyll a/b-binding protein promoter and introduced into rice. PpRbcS was highly expressed in leaf blade and accounted for approximately 30 % of total RbcS in homozygous transgenic lines. However, the catalytic turnover rate and K m for CO2 of Rubisco did not significantly change in these transgenic lines compared to non-transgenic rice, suggesting that PpRbcS1 is not effective for improvement of catalytic efficiency of rice Rubisco. The photosynthetic rate and growth were essentially unchanged, whereas the photosynthetic rate at low CO2 condition was marginally increased in transgenic lines. Rubisco content was significantly increased, whereas soluble protein, nitrogen, and chlorophyll contents were unchanged in transgenic lines compared to non-transgenic rice. Because the kinetic properties were similar, observed slight increase in photosynthetic rate at low CO2 is considered to be large due to increase in Rubisco content in transgenic lines. Introduction of foreign RbcS is an effective approach for the improvement of Rubisco kinetics and photosynthesis. However, in this study, it was suggested that RbcS of high activity-type Rubisco, even showing higher amino acid identity with rice RbcS, did not always enhance the catalytic turnover rate of Rubisco in rice. Thus, we should carefully select RbcS to be overexpressed before introduction.
Kluwer Academic Publishers, 2015年, Photosynthesis Research, 124 (1), 57 - 65, 英語[査読有り]
研究論文（学術雑誌）
Phosphoenolpyruvate carboxylase (PEPC) undergoes activity regulation through reversible phosphorylation. The day/night phosphorylation of leaf PEPC in 27 C-3 plant species was analyzed by immunoblotting. PEPC was phosphorylated in the daytime in 12 species, whereas it was phosphorylated at night in three species, rice, Monochoria vaginalis, and Sagittaria trifolia, all of which are hygrophytic monocots. Immunoblot analysis of isolated chloroplasts of M. vaginalis identified a PEPC protein inside the chloroplast in addition to cytosolic isozyme(s) as previously shown in genus Oryza. Using transgenic rice overexpressing the maize PEPC in the cytosol, we confirmed that the cytosolic PEPC underwent the nocturnal phosphorylation. These results suggest the interrelationship between the presence of chloroplastic PEPC and the nocturnal phosphorylation of cytosolic isozyme(s).
TAYLOR & FRANCIS LTD, 2014年04月, Bioscience Biotechnology and Biochemistry, 78 (4), 609 - 613, 英語[査読有り]
研究論文（学術雑誌）
Rubisco small subunits (RbcSs) are encoded by a nuclear multigene family in plants. Five RbcS genes, OsRbcS1, OsRbcS2, OsRbcS3, OsRbcS4, and OsRbcS5, have been identified in rice (Oryza sativa). Among them, the amino acid sequence of OsRbcS1 differs notably from those of other rice RbcSs. Phylogenetic analysis showed that OsRbcS1 is genetically distant from other rice RbcS genes and more closely related to RbcS from a fern and two woody plants. Reverse transcription-PCR and promoter beta-glucuronidase analyses revealed that OsRbcS1 was not expressed in leaf blade, a major photosynthetic organ in rice, but was expressed in leaf sheath, culm, anther, and root central cylinder. In leaf blade of transgenic rice overexpressing OsRbcS1 and leaf sheath of nontransgenic rice, OsRbcS1 was incorporated into the Rubisco holoenzyme. Incorporation of OsRbcS1 into Rubisco increased the catalytic turnover rate and K-m for CO2 of the enzyme and slightly decreased the specificity for CO2, indicating that the catalytic properties were shifted to those of a high-activity type Rubisco. The CO2 assimilation rate at low CO2 partial pressure was decreased in overexpression lines but was not changed under ambient and high CO2 partial pressure compared with nontransgenic rice. Although the Rubisco content was increased, Rubisco activation state was decreased in overexpression lines. These results indicate that the catalytic properties of Rubisco can be altered by ectopic expression of OsRbcS1, with substantial effects on photosynthetic performance in rice. We believe this is the first demonstration of organ-specific expression of individual members of the RbcS gene family resulting in marked effects on Rubisco catalytic activity.
American Society of Plant Biologists, 2014年01月, Plant Physiology, 164 (1), 69 - 79, 英語[査読有り]
研究論文（学術雑誌）
Diacylglycerol acyltransferase (DGAT) catalyzes the acyl-CoA-dependent acylation of sn-1,2-diacylglycerol to form seed oil triacylglycerol (TAG). To understand the features of genes encoding soybean (Glycine max) DGATs and possible roles in soybean seed oil synthesis and accumulation, two full-length cDNAs encoding type 1 diacylglycerol acyltransferases (GmDGAT1A and GmDGAT1B) were cloned from developing soybean seeds. These coding sequences share identities of 94 % and 95 % in protein and DNA sequences. The genomic architectures of GmDGAT1A and GmDGAT1B both contain 15 introns and 16 exons. Differences in the lengths of the first exon and most of the introns were found between GmDGAT1A and GmDGAT1B genomic sequences. Furthermore, detailed in silico analysis revealed a third predicted DGAT1, GmDGAT1C. GmDGAT1A and GmDGAT1B were found to have similar activity levels and substrate specificities. Oleoyl-CoA and sn-1,2-diacylglycerol were preferred substrates over vernoloyl-CoA and sn-1,2-divernoloylglycerol. Both transcripts are much more abundant in developing seeds than in other tissues including leaves, stem, roots, and flowers. Both soybean DGAT1A and DGAT1B are highly expressed at developing seed stages of maximal TAG accumulation with DGAT1B showing highest expression at somewhat later stages than DGAT1A. DGAT1A and DGAT1B show expression profiles consistent with important roles in soybean seed oil biosynthesis and accumulation.
SPRINGER HEIDELBERG, 2013年03月, Functional & Integrative Genomics, 13 (1), 99 - 113, 英語[査読有り]
研究論文（学術雑誌）
- Photosynthetic characteristics of antisense transgenic rice expressing reduced levels of Rubisco activase
The activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which catalyses CO2 fixation in photosynthesis, requires the assistance of the regulatory protein Rubisco activase. Rubisco activase promotes carbamylation of Rubisco by releasing inhibitory sugar phosphates bound to the catalytic site of Rubisco in the light. To clarify the effects of Rubisco activase contents on the photosynthesis of rice, we investigated the steady-state photosynthesis and light-induction of photosynthesis in transgenic rice plants, in which leaf Rubisco activase levels were reduced. The reduction in Rubisco activase did not affect steady-state photosynthesis under high light intensity until the Rubisco activase was about 15% of that in control plants. However, light-induction of photosynthesis, namely, increase in photosynthetic rate following a transition from a low to high light intensity, was considerably low in transgenic rice plants with 20-25% Rubisco activase, which was sufficient to support the steady-state photosynthesis. In addition, the Rubisco activase content was highly correlated with the initial rate of Rubisco activation after the increase in light intensity. These results suggest that Rubisco activase in rice leaves largely limits the light-induction of photosynthesis, but not steady-state photosynthesis.
CROP SCIENCE SOC JAPAN, 2012年07月, Plant Production Science, 15 (3), 174 - 182, 英語[査読有り]
研究論文（学術雑誌）
The effects of overexpression of Rubisco activase on photosynthesis were studied in transgenic rice expressing barley or maize Rubisco activase. Immunoblot and SDS-PAGE analyses showed that transgenic lines from both gene constructs expressed the foreign Rubisco activase at high levels. The activation state of Rubisco in transgenic lines was slightly higher than that in non-transgenic plants (NT). In addition, light activation of Rubisco was significantly more rapid in transgenic lines compared with NT. These findings indicate that the overexpression of Rubisco activase can enhance Rubisco activation. However, despite enhanced activation of Rubisco in these transgenic plants, the CO2 assimilation rate at ambient CO2 conditions was decreased. This decrease in CO2 assimilation rate was observed in both young developing and mature leaves independent of nitrogen nutrition. The contents of nitrogen and Chl did not differ significantly between transformants and NT; however, Rubisco content was substantially decreased in transgenic lines. There was no evidence for reduced transcription of RbcS or RbcL in these transgenic lines; in fact, transcript levels were marginally increased compared with NT. These results indicate that the overexpression of Rubisco activase leads to a decrease in Rubisco content, possibly due to post-transcriptional mechanisms.
OXFORD UNIV PRESS, 2012年06月, Plant and Cell Physiology, 53 (6), 976 - 986, 英語[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2012年01月, Metabolic Engineering, 14 (1), 29 - 38
[査読有り]
研究論文（学術雑誌）
Abstract Rubisco limits photosynthetic CO2 fixation because of its low catalytic turnover rate (k cat) and competing oxygenase reaction. Previous attempts to improve the catalytic efficiency of Rubisco by genetic engineering have gained little progress. Here we demonstrate that the introduction of the small subunit (RbcS) of high k cat Rubisco from the C4 plant sorghum (Sorghum bicolor) significantly enhances k cat of Rubisco in transgenic rice (Oryza sativa). Three independent transgenic lines expressed sorghum RbcS at a high level, accounting for 30%, 44%, and 79% of the total RbcS. Rubisco was likely present as a chimera of sorghum and rice RbcS, and showed 1.32- to 1.50-fold higher k cat than in nontransgenic rice. Rubisco from transgenic lines showed a higher K m for CO2 and slightly lower specificity for CO2 than nontransgenic controls. These results suggest that Rubisco in rice transformed with sorghum RbcS partially acquires the catalytic properties of sorghum Rubisco. Rubisco content in transgenic lines was significantly increased over wild-type levels but Rubisco activation was slightly decreased. The expression of sorghum RbcS did not affect CO2 assimilation rates under a range of CO2 partial pressures. The J max/V cmax ratio was significantly lower in transgenic line compared to the nontransgenic plants. These observations suggest that the capacity of electron transport is not sufficient to support the increased Rubisco capacity in transgenic rice. Although the photosynthetic rate was not enhanced, the strategy presented here opens the way to engineering Rubisco for improvement of photosynthesis and productivity in the future.
Oxford University Press (OUP), 2011年05月11日, Plant Physiology, 156 (3), 1603 - 1611[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2011年02月, Field Crops Research, 121 (1), 195 - 199
[査読有り]
研究論文（学術雑誌）
- 2010年08月, The 15th International Congress of Photosynthesis, 144, 英語RbcS increases the catalytic turnover rate of Rubisco in transgenic rice plants
研究論文（国際会議プロシーディングス）
- Wiley, 2010年02月, Plant Biotechnology Journal, 8 (2), 184 - 195
[査読有り]
研究論文（学術雑誌）
- Screening of high k(cat) Rubisco among Poaceae for improvement of photosynthetic CO2 assimilation in rice
The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a major limiation of photosynthetic CO2 assimilation in C-3 plants. In order to find useful Rubisco for improvement of photosynthesis in rice under elevated CO2, we analyzed the catalytic turnover rate (k(cat)) of Rubisco in Poaceae including C-3 alpine plants, C-3 cold-resistant plants and C-4 plants. Rubisco in these plants showed 1.1- to 2.8-fold higher k(cat) than that in rice. However, the most of high k(cat) Rubisco also showed a higher km for CO2 (Kc) than that of rice, indicating that increase in k(cat) led to decrease in the affinity for CO2. Rubisco in Festuca ovina, Phleum pratense and Sorghum bicolor showed relatively high k(cat) to Kc. Although the k(cat) of Rubisco in F. ovina and P. pralense was not so high (1.5-1.6 fold relative to rice), the Kc was comparable to that in rice and the amino acid sequence of RbcL shared higher identity to that in rice than that in S. bicolor. By contrast, Rubisco of S. bicolor showed considerably high k(cat) (2.5-fold relative to rice), which is considered to be the most improtant factor for improvement of photosynthesis. In our estimation, the expression of high k(cat) Rubisco of F. ovina and S. bicolor in rice could significantly enhance CO2 assimilation at Ci and 50 Pa, the level assumed to be the middle of this century.
CROP SCIENCE SOC JAPAN, 2009年07月, Plant Production Science, 12 (3), 345 - 350, 英語[査読有り]
研究論文（学術雑誌）
- 2009年, Hildebrand D, Li R, Yu K, Hatanaka T, 2007 (Nov.), 33 - 42, 英語植物油脂におけるエポキシ脂肪酸の蓄積
研究論文（国際会議プロシーディングス）
- Identification of Black Soybean (Glycine max) Tanbaguro and Processed Foods by Simple Sequence Repeat Analysis
"Tanbaguro" (Glycine max) is a local variety of Japanese soybean characterized by a large seed size and black seed coat. We examined a method to distinguish Tanbaguro seeds, which were maintained and managed in domestic production as the standard strains, from seeds of other black soybean varieties. We selected 18 pairs of soybean simple sequence repeat (SSR) primers as primer sets to distinguish the seed varieties. Our analysis revealed that 4 strains and 20 seeds cultivated in Japan and distributed as Tanbaguro exhibited total agreement with standard strains, while 12 strains and 60 seeds cultivated in China and distributed as Tanbaguro exhibited only 13% agreement. These results suggested a wide genetic diversity of black soybeans cultivated in China. We then confirmed that our identification method using the same 18 primer sets can also be applied to processed foods. Identification of Tanbaguro products that were boiled, steamed and roasted was as successful as that of seeds; the method could not be applied to fermented (natto) Tanbaguro products. Analysis of commercially processed foods containing Tanbaguro indicated that some foods of unknown source included soybeans cultivated in China, confirming that these foods are mixtures of Tanbaguro standard strains and other varieties.
JAPAN SOC FOOD SCIENCE TECHNOLOGY, 2009年, JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 56 (3), 119 - 128, 日本語[査読有り]
研究論文（学術雑誌）
- 日本食品工学会, 2009年, 日本食品工学会誌, 56, 119 - 128, 日本語SSR分析を用いた黒大豆「丹波黒」とその加工品の品種判別
[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2008年03月, Phytochemistry, 69 (5), 1119 - 1127
[査読有り]
研究論文（学術雑誌）
- 近畿作物・育種研究会, 2008年, 作物研究, 53, 47 - 53, 日本語丹波黒系黒ダイズ標準化のためのSSRマーカーによる遺伝的近縁性評価
[査読有り]
研究論文（学術雑誌）
- 無機元素組成による黒大豆「丹波黒」の産地判別
"Tanbaguro" is a local variety of Japanese soybean (Glycine max) that is characterized by large seed size and a black seed coat. We intended to determine a method for determining the geographic origin of Tanbaguro seeds by their inorganic element composition. Seed samples of 48 Tanbaguro varieties and eight "non-Tanbaguro" black soybean varieties cultivated in different years were collected in Japan and China. Eight inorganic elements (Al, Mn, Fe, Ni, Cu, Zn, Sr and Ba) of these samples were estimated using ICP-AES (Inductively coupled plasma atomic emission spectrometer). Significant differences were observed between Japanese and Chinese samples in the contents of four elements (Al, Fe, Cu and Sr). Principal component analysis samples could generally be divided into two groups : those cultivated in Japan and those cultivated in China. Various combinations of inorganic elemental contents were used to perform the linear discriminant analysis (LDA). LDA performed by eight elements classified with a confidence of 96%, and country of origin could be predicted with confidence of 100%. Even when four elements (Al, Mn, Sr and Ba) were used, it was possible to distinguish whether the country of cultivation was Japan or China. LIDA performed by Al and Fe contents, selected by stepwise discriminant analysis, classified and predicted with a confidence of only 89% and 79%. However, since all samples cultivated in China could be correctly classified and predicted, this discriminant model was useful for the screening of samples. Therefore, the applicability of this method for determining the geographic origin of Tanbaguro bean varieties was demonstrated.
JAPAN SOC FOOD SCIENCE TECHNOLOGY, 2006年, 日本食品化学工学会誌, 53 (6), 344 - 353, 日本語[査読有り]
研究論文（学術雑誌）
- Mechanism of high photosynthetic capacity in BC2F4 lines derived from a cross between Oryza sativa and wild relatives O rufipogon
We found that several BC2F4 lines had high leaf photosynthetic rates Under light-saturated and ambient CO2 conditions. These lines are progenies of BC,F, plants with high photosynthetic capacities which were generated by backcrossing between Oryza rufipogon (W630) and O. saliva cv. Nipponbare, as a recurrent parent. Some photosynthetic characteristics of the BC2F4, lines were investigated to identify the factors increasing photosynthetic rates. Photosynthetic rates of these lines under light-saturated conditions at 50 to 700 ppm CO2 concentrations were higher than those in Nipponbare. The estimated-maximum photosynthetic rates under light-saturated and CO2-saturated conditions in BC2F4 lines were also higher than that in Nipponbare. The photosynthetic rate under light-saturated and ambient CO2 conditions was positively correlated with the carboxylation efficiency as an indicator of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity in vivo rather than stomatal conductance. Initial and total Rubisco activities in vitro tended to be higher in the BC2F4 lines than in Nipponbare. The content of active Rubisco calculated from the activation state of Rubisco was also higher in the BC2F4 lines than in Nipponbare. These results suggest that high photosynthetic capacities of BC2F1 plants can be maintained high in their progenies and high photosynthetic rates Under light-saturated and ambient CO2 conditions in the BC2F4 lines are achieved mainly by the high activity of Rubisco due to the high active Rubisco content.
CROP SCIENCE SOC JAPAN, 2005年12月, Plant Production Science, 8 (5), 539 - 545, 英語[査読有り]
研究論文（学術雑誌）
- Enhancement of rice leaf photosynthesis by crossing between cultivated rice, Oryza sativa and wild rice species, Oryza rufipogon
To study whether wild rice species have genes that may increase potential photosynthetic capacities of rice cultivars, we generated BC, populations by reciprocally backcrossing Oryza rufipogon (W630) with O. sativa cv. Nipponbare and IR36; N-BC2 populations and IR-BC2 populations, respectively. We measured the oxygen evolution rates (OER) of single leaves under saturating light and CO2 as the maximum photosynthetic rates and the contents of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase. Several lines in each BC, population had significantly higher OERs than parental cultivars, and 14similar to25% of plants in BC2 populations had higher OERs than the highest values in parental cultivars. The highest OERs in BC2 populations were about 60% higher than average OERs in parental cultivars. The BC2 populations contained 30-40% more Rubisco than parental cultivars. The Rubisco activase contents in N-BC2 populations were 15-30% lower than that in Nipponbare. Cytoplasms derived from O. rufipogon and O. sativa had different effects on the contents of Rubisco and Rubisco activase particularly in N-BC2 populations. In several lines of each BC2 population the OERs had positive correlations with the contents of Rubisco and/or Rubisco activase. These results suggest that O. rufipogon can be used as a source of germplasm to enhance the photosynthetic capacity of O. sativa.
CROP SCIENCE SOC JAPAN, 2004年09月, Plant Production Science, 7 (3), 252 - 259, 英語[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2004年08月, Phytochemistry, 65 (15), 2189 - 2196
[査読有り]
研究論文（学術雑誌）
- 2004年, Proceedings of the 4th International Crop Science Congress, 26, 英語Tanbaguro: a new model genotype of soybean for tissue culture study.
研究論文（国際会議プロシーディングス）
- 2004年, Proceedings of the 4th International Crop Science Congress, 26, 英語Photosynthesis, growth and yield of BC2F4 lines derived from Oryza sativa and wild rice species, O. rufipogon
研究論文（国際会議プロシーディングス）
- Interactions between abscisic acid, ethylene and gibberellin in internodal elongation in floating rice: the promotive effect of abscisic acid at low humidity
The enhancement of internodal elongation in floating or deepwater rice (Oryza sativa L. cv. Habiganj Aman II) by treatment with ethylene or gibberellic acid (GA(3)) at high relative humidity (RH) is inhibited by abscisic acid (ABA). Here, we examined the interactive effects of ethylene, gibberellin (GA) and ABA at low RH on internodal elongation of deepwater rice stem segments. Although ethylene alone hardly promoted internodal elongation of stem sections at 30% RH, it enhanced the internodal elongation induced by GA(3). Application of ABA alone to stem segments had no effect on internodal elongation. However, in the presence of ethylene and GA(3) at 30% RH, ABA further promoted internodal elongation. This promotive effect of ABA was not found in the internodes of stem segments treated either with ethylene or with GA(3) at 30% RH or in the internodes of stem segments treated with ethylene and/or GA(3) at 100% RH.
KLUWER ACADEMIC PUBL, 2003年10月, Plant Growth Regulation, 41 (2), 105 - 109, 英語[査読有り]
研究論文（学術雑誌）
- Enhancement of transpiration by ethylene is responsible for absence of internodal elongation in floating rice at low humidity
Internodal elongation in floating rice (Oryza sativa) is known to be enhanced by treatment with ethylene or gibberellic acid (GA(3)) at high relative humidity (RH). However, ethylene-induced internodal elongation is inhibited at low RH, while GA(3)-induced internodal elongation is hardly affected by humidity. We examined the effects of ethylene and GA3 on the rate of transpiration in stem segments incubated at 30 % or 100 % RH. Ethylene promoted the transpiration of stem segments at 30 % RH. but not at 100% RH, while GA(3) had little effect on transpiration at either 30% or 100% RH. We propose that the absence of ethylene-induced internodal elongation at low RH is due, at least in part, to ethylene-induced transpiration.
URBAN & FISCHER VERLAG, 2003年09月, Journal of Plant Physiology, 160 (9), 1125 - 1128, 英語[査読有り]
研究論文（学術雑誌）
- SPRINGER, 2003年, Advanced Research on Plant Lipids, 155 - 158, 英語Cloning and expression of Vernonia and Euphorbia diacylglycerol acyltransferase cDNAs
研究論文（国際会議プロシーディングス）
- 2002年, Environmental Control in Biology, 40, 195 - 200, 英語Varietal differences of rice (Oryza sativa L.) growth to low nitrogen supply
[査読有り]
研究論文（学術雑誌）
異なる3段階の窒素施肥量下で水耕栽培したインド型イネ8品種の光合成能力とRubisco含量との関係を調べた.酸素電極法による最大光合成能力とRubisco含量との間には, 含量が2gm-2以下では比例関係があり, それ以上の含量では飽和する傾向がみられた.Pokkali, BaisbishとIR442-2-58はRubisco量当りの光合成能力が高い品種であった.比例域での回帰分析の結果, イネ葉の最大光合成能力に対するRubiscoの効率には品種間差異があり, その効率が高い品種群と低い品種群とに大きく区分された.
Japanese Society for Tropical Agriculture, 2002年, 熱帯農業, 46 (3), 162 - 165, 英語[査読有り]
- 2002年, Environmental Control in Biology, 40 (2), 201 - 206, 英語Partitioning of absorbed nitrogen to chloroplast, soluble protein and rubisco in rice leaves under low nitrogen supply.
[査読有り]
研究論文（学術雑誌）
- Transgenic plants of coffee Coffea canephora from embryogenic callus via Agrobacterium tumefaciens-mediated transformation
Embryogenic calli were induced from leaf explants of coffee (Coffea canephora) on McCown's woody plant medium (WPM) supplemented with 5 mu M N-6-(2-isopentenyl)-adenosine (2-iP). These calli were co-cultured with Agrobacterium tumefaciens EHA101 harboring pIG121-Hm, containing beta-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransferase II genes. Selection of putative transgenic callus was performed by gradual increase in hygromycin concentration (5, 50, 100 mg/l). The embryogenic calli surviving on medium containing 100 mg/l hygromycin showed a strong GUS-positive reaction with X-Gluc solution. Somatic embryos were formed from these putative transgenic calli and germinated on WPM medium with 5 mu M 2-iP. Regenerated small plantlets with shoots and roots were transferred to medium containing both 100 mg/l hygromycin and 100 mg/l kanamycin for final selection of transgenic plants. The selected plantlets exhibited strong GUS activity in leaves and roots as indicated by a deep blue color. GUS and HPT genes were confirmed to be stably integrated into the genome of the coffee plants by the polymerase chain reaction.
SPRINGER VERLAG, 1999年12月, Plant Cell Reports, 19 (2), 106 - 110, 英語[査読有り]
研究論文（学術雑誌）
- Molecular cloning and characterization of coffee cDNA encoding spermidine synthase
A cDNA for spermidine synthase (SPDS), which converts putrescine to spermidine using decarboxylated S-adenosylmethionine as a co-factor, has been isolated from Coffea arabica. When the SPDS cDNA is expressed in an SPDS-deficient E. coli mutant: the recombinant protein shows high SPDS activity. The C. arabica SPDS possesses the co-factor binding motifs which have been proposed for S-adenosylmethionine, and its amino acid sequence is similar to other plant SPDSs. The SPDS transcripts have been observed in roots, green stems, old and young leaves, and accumulated to a higher level in rapid growing tissues, such as green stems and younger leaves, compared to old leaves. In callus tissues, it has been expressed in all stages, even though callus growth is very slow during somatic embryogenesis. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
ELSEVIER SCI IRELAND LTD, 1999年01月, PLANT SCIENCE, 140 (2), 161 - 168, 英語[査読有り]
研究論文（学術雑誌）
- 1996年, Seed propagation of Habenaria radiata. Molphological and physiological characteristics of plants derived form in vitro cultured seedlings., 1, 67 - 81, 英語Seed propagation of Habenaria radiata. Molphological and physiological characteristics of plants derived form in vitro cultured seedlings.
[査読有り]
研究論文（大学，研究機関等紀要）
- The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora
The role of ethylene in the formation of somatic embryos from leaf discs of Coffea canephora was studied on a medium that contained a cytokinin, iso-pentenyladenine, as the sole plant growth regulator. During incubation under these conditions, explants always produced a small amount of ethylene. Removal of this ethylene by an absorbent reduced the number of somatic embryos induced by the cytokinin, Application of inhibitors of the production of ethylene (Co2+ ions) and of the action of ethylene (Ag+ ions) inhibited the formation of embryos. Exogenous ethylene gas (12 mu l/l) partially overcame the effect of Co2+ ions. These results indicate that ethylene plays an important role in regulating somatic embryogenesis in leaf cultures of Coffea canephora.
ELSEVIER SCI PUBL IRELAND LTD, 1995年06月, Plant Science, 107 (2), 199 - 204, 英語[査読有り]
研究論文（学術雑誌）
- ASSOC SCIENTIFIQUE INT CAFE, 1995年, 16th International Scientific Colloquium On Coffee, VOLS I & II, 16, 790 - 797, 英語Effect of plant hormones on somatic embryogenesis of Coffea canephora
研究論文（国際会議プロシーディングス）
- ASSOC SCIENTIFIQUE INT CAFE, 1995年, 16th International Scientific Colloquium On Coffee, VOLS I & II, 16, 537 - 541, 英語Clonal propagation through somatic embryogenesis of Coffea species
研究論文（国際会議プロシーディングス）
- Direct regrowth of encapsulated somatic embryos of coffee (Coffea canephora) after cooling in liquid nitrogen
Somatic embryos of Coffea canephora were successfully cryopreserved after being exposed to a series of preparative steps. 1) Somatic embryos were progressively precultured on media with increasing concentrations of sucrose (03, 0.5, 0.7 and 0.8 M), growing for 3-4 days on each medium. 2) They were then encapsulated in alginate beads that contained 0.5 M sucrose. 3) These beads were dehydrated up to about 13% (FW basis) and plunged into liquid nitrogen. Sixty-three percent of cryopreserved embryos remained alive and about 50% of them grew directly without formation of secondary embryos and calluses. The remaining meristems from cryopreserved material ceased to grow after rooting. This cryogenic procedure appears to be a promising technique for cryopreservation of coffee somatic embryos.
CRYO LETTERS, 1994年01月, Cryo-Letters, 15 (1), 47 - 52, 英語[査読有り]
研究論文（学術雑誌）
- 兵庫県立人と自然の博物館, 1993年, 人と自然, 2, 119 - 124, 日本語組織培養によるムロウテンナンショウ Arisaema yamatense Nakai（サトイモ科）の増殖
[査読有り]
研究論文（大学，研究機関等紀要）
- Effect of plant growth regulators on somatic embryogenesis in leaf cultures of Coffea canephora
The effects of plant growth regulators on somatic embryogenesis were studied in leaf cultures of Coffea canephora. The maximum number of somatic embryos were obtained on media that contained only cytokinin as a plant growth regulator. All of the auxins tested (NAA, IBA, IAA and 2,4-D) inhibited the formation of embryos. The optimal concentration of each cytokinin (2-iP, BA and kinetin) for somatic embryogenesis was 5-mu-M. Under optimal conditions, each explant formed more than 100 embryoids with little callus and few adventitious roots. Embryoids were formed only at the cut edges of the leaf discs. Cytokinins were absorbed only at the cut edges of leaf discs that were in contact with the medium, and were not transported to other parts of the explant.
SPRINGER VERLAG, 1991年07月, Plant Cell Reports, 10 (4), 179 - 182, 英語[査読有り]
研究論文（学術雑誌）
MISC
- 2016年, 日本作物学会講演会要旨集, 241st高CO2条件で育成したソルガムRbcS高発現・イネRbcS発現抑制2重形質転換イネの生理特性
- 2014年, 日本作物学会講演会要旨・資料集, 237thソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法でRubisco含量を減少させた二重形質転換イネの光合成特性
- 2013年09月09日, 日本作物學會紀事, 82, 84 - 85, 日本語耐塩性イネ品種 Pokkali のF_1倍加半数体系統群における幼苗期耐塩性指標の評価
- 2013年03月28日, 日本作物學會紀事, 82, 182 - 183, 日本語イネにおいて葉身で発現しない新規 Rubisco 小サブユニットOsRbcS1の機能解析
- 2013年03月28日, 日本作物學會紀事, 82, 184 - 185, 日本語Rubisco activase は Rubisco 生合成を阻害することにより Rubisco の発現を抑制する
- 2013年03月28日, 日本作物學會紀事, 82, 186 - 187, 日本語高CO_2条件で発現促進される新規CCTドメインタンパク質CRCTは分げつ角度と基本代謝酵素の発現調節に働く
- 2012年03月29日, 日本作物學會紀事, 81, 146 - 147, 日本語高CO_2条件で発現促進される受容体型キナーゼOsTPK1の機能解析
- 2012年03月29日, 日本作物學會紀事, 81, 318 - 319, 日本語幼苗期におけるインディカイネの交雑親と倍加半数体系統群の耐塩性比較
- 2011年03月30日, 日本作物學會紀事, 80, 144 - 145, 日本語イネにおける葉緑体型ホスホグリセリン酸ムターゼOsPGMd2の機能解析
- 2011年03月30日, 日本作物學會紀事, 80, 142 - 143, 日本語新規なCCTドメインタンパク質OsCCT1は高CO_2環境への代謝的・形態的応答に関係している
- 2010年03月30日, 日本作物學會紀事, 79, 102 - 103, 日本語イネにおけるプラスチド局在型エノラーゼ遺伝子の機能解析
- 2009年03月27日, 日本作物學會紀事, 78, 214 - 215, 日本語オオムギ Rubisco activase の高発現がイネの光合成特性と葉内成分含量におよぼす効果
- 日本作物学会, 2008年09月24日, 日本作物學會紀事, 77 (2), 292 - 293, 日本語P-39 丹波黒大豆'兵系黒3号'の成熟培養における,糖とアミノ酸の効果(日本作物学会第226回講演会)
- 日本作物学会, 2008年09月24日, 日本作物學會紀事, 77 (2), 294 - 295, 日本語P-40 油料作物Jatropha curcasの組織培養による増殖と脂質定量(日本作物学会第226回講演会)
- 日本作物学会, 2008年09月24日, 日本作物學會紀事, 77 (2), 262 - 263, 日本語P-24 イネの光合成能力改良に向けたイネ科植物内における高k_
Rubiscoの探索(日本作物学会第226回講演会) - 日本作物学会, 2005年03月30日, 日本作物學會紀事, 74 (1), 96 - 97, 日本語48 野生イネOryza rufipogon由来の光合成能力関連QTL領域をもつ準同質遺伝子系統の光合成特性(光合成・代謝)
- 日本作物学会, 2004年10月20日, 日本作物學會紀事, 73 (2), 324 - 325, 日本語P-23 栽培イネOrysa sativa×野生イネO.rufipogonのBC_2F_4系統の光合成特性
- 日本作物学会, 2003年09月17日, 日本作物學會紀事, 72 (2), 304 - 305, 日本語浮稲節間の伸長とエチレンによる蒸散の促進(日本作物学会第216回講演会要旨・資料集)
- 日本作物学会, 2003年, 日本作物学会紀事, 72 (1), 184 - 185, 日本語SSR-PCR法による丹波黒の判別 (I)
- 光琳, 2000年, 食品工業, 43 (6), 55 - 64, 日本語コーヒー生産の科学 第３章 コーヒーのバイオテクノロジー
記事・総説・解説・論説等（学術雑誌）
- 光琳, 2000年, 食品工業, 43 (4), 61 - 70, 日本語コーヒー生産の科学 第２章 さまざまなコーヒー
記事・総説・解説・論説等（学術雑誌）
- 1995年03月, Plant and cell physiology, 36, S109, 英語ROLE OF ETHYLENE IN SOMATIC EMBRYOGENESISOF COFFEA CANEPHORA
- 1993年, 植物細胞工学, 5, 473 - 477, 日本語組織培養のepoch-making 2 - コーヒーの組織培養 -
記事・総説・解説・論説等（学術雑誌）
書籍等出版物
- その他, 朝倉書店, 2017年07月, 日本語作物栽培大系7 工芸作物の栽培と利用 3章-7 ヤトロファ（ジャトロファ）
学術書
- その他, CRC Press, New York, N.Y., 2009年, 英語Accumulation of epoxy fatty acids in plant oils. in eds. Hou, C.T., Shaw, J-F. Biocatalysis and Agricultural Biotechnology
学術書
- 共著, Springer, New York, N.Y., 2008年, 英語Genomics of soybean oil traits. in Genetics and Genomics of Soybean.
学術書
- その他, 農業生物資源研究所, 2006年03月, 日本語植物超低温保存マニュアル
学術書
- その他, Marcel Dekker, Inc., New York, 2002年, 英語Redirecting Lipid Metabolism in Plants. in Lipid Biotechnology.
学術書
講演・口頭発表等
- 第34回 日本植物脂質研究会 シンポジウム, 2022年09月21日高等植物DGAT1活性を上げる 特定のアミノ酸残基について
口頭発表（一般）
- 第33回植物脂質シンポジウム, 2021年09月22日, 日本語植物DGAT1活性の違いに影響するアミノ酸残基
口頭発表（一般）
- 日本作物学会 第252回講演会, 2021年09月10日, 日本語外生WRI1発現は種子油の含有量と脂肪酸組成に 影響を及ぼす
口頭発表（一般）
- The 10th Asian Crop Science Association Conference, 2021年09月09日, 英語DGAT1s from Different Plant Species Show Different Triacylglycerol Biosynthesis Activities
ポスター発表
- 第10回アジア作物学会, 2021年09月09日, 英語Highly Active Vernonia galamensis DGAT1 Can Effectively Increase Oil Levels in Yeast, Soybean and Arabidopsis
口頭発表（一般）
- 近畿作物・育種研究会 第189回例会, 2020年12月12日, ジャワ語Oryza sativa/Oryza glumaepatula染色体置換系統の浮稲性の解析
- 近畿作物・育種研究会 第189回例会, 2020年12月12日, 日本語イネ冠根の成長方向に及ぼす環境シグナルとエチレンの影響
- The 8th Asian - Oceanian Symposium on Plant Lipids, 2019年11月20日, 英語, 国際会議DGAT1 from different species show different triacylglycerol biosynthesis activities
ポスター発表
- International Symposium on Biocatalysis and Agricultural Biotechnology, 2019年09月19日, 英語, 国際会議DGAT1s from Different Plant Species Show Different Triacylglycerol Biosynthesis Activities
[招待有り]
口頭発表（招待・特別）
- 種子生理生化学研究会, 2018年11月, 日本語, 花巻温泉, 国内会議和歌山市で育てた5品種の亜麻の種子成分の品種間差 ―亜麻(Linum usitatissimum)の観光資源としての可能性の検討
口頭発表（一般）
- 第31回植物脂質シンポジウム, 2018年11月, 日本語, 高知大学, 国内会議Vernonia galamensis DGAT1は他種のDGAT1より シロイヌナズナ種子油含量を上げることができる
ポスター発表
- The 14th International Symposium on Biocatalysis and Agricultural Biotechnology, 2018年10月, 英語, Kuala Lumpur, Malaysia, 国際会議Vernonia galamensis DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s
ポスター発表
- International Seminar on Malaysian Palm Oil Board, 2018年10月, 英語, 国際会議Triacylglycerol biosynthesis in plants
[招待有り]
口頭発表（招待・特別）
- 日本作物学会第246回講演会, 2018年09月, 日本語, 北海道大学農学部, 国内会議南米野生イネOryza glumaepatulaの浮稲反応の解析
ポスター発表
- 日本作物学会第246回講演会, 2018年09月, 日本語, 北海道大学農学部, 国内会議イネRubiscoのC4化と構造機能相関
口頭発表（一般）
- 日本作物学会第246回講演会, 2018年09月, 日本語, 北海道大学農学部, 国内会議アフリカイネOryza glaberrimaとその祖先種Oryza barthiiの洪水に対する応答
ポスター発表
- The 23rd International Symposium on Plant Lipids, 2018年07月, 英語, Yokohama, Japan, 国際会議Highly active Vernonia galamensis DGAT1 can effectively increase oil levels in yeast, soybean and Arabidopsis
ポスター発表
- 近畿作物・育種研究会 第185回例会, 2018年06月, 日本語, 国内会議アフリカイネ Oryza glaberrima とその祖先種 O. barthii の洪水に対する応答
口頭発表（一般）
- 日本作物学会講演会, 2018年, 日本語, 国内会議シロイヌナズナにおける CRCT オーソログの機能解析
ポスター発表
- 日本作物学会講演会, 2018年, 日本語, 国内会議イネの Rubisco activase 様タンパク質 Rca2 は Rubisco activase の機能を阻害する
ポスター発表
- 日本光合成学会講演会, 2018年, 日本語, 国内会議イネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害する
ポスター発表
- International Symposium on Photosynthesis and Chloroplast Biogenesis, 2018年, 英語, 国内会議Functional analysis of Rubisco small subunit, OsRbcS1, that is not expressed in photosynthetic organs in rice
ポスター発表
- 日本作物学会講演会, 2018年, 日本語, 国内会議C4植物の Rubisco 小サブユニットを高発現する形質転換イネの Rubisco 酵素特性
ポスター発表
- The 7th Asian Symposium on Plant Lipid, 2017年11月, 英語, Taipei, Taiwan, 国際会議Highly active Vernonia galamensis DGAT1 can effectively increase seed oil levels in soybean and Arabidopsis
ポスター発表
- 日本作物学会第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ品種の深水成長応答と関連遺伝子の発現
ポスター発表
- 日本作物学会第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議南米野生イネOryza glumaepatulaの深水に対する応答とSNORKEL遺伝子の多様性
ポスター発表
- 日本作物学会第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議深水環境下において浮稲節間の伸長を誘導する新規因子の探索
ポスター発表
- 日本作物学会 第244回講演会, 2017年09月, 日本語, 岐阜大学, 国内会議ベルノニアのDGAT1はシロイヌナズナにおいても他種のDGAT1より高い活性を示すか？
ポスター発表
- 第30回植物脂質シンポジウム, 2017年09月, 日本語, お茶の水大学, 国内会議シロイヌナズナにおけるベルノニアDGAT1の働き
ポスター発表
- Plant Biology 2017, 2017年06月, 英語, Honolulu, Hawaii, USA, Plant oils represent one of the world’s most important renewable resources. In addition to extensive food uses, vegetable oils also have value as renewable chemical derivatives and fuels, such as biodiesel. Worldwide production of plant oil has been increasing. Triacylglycerols (TAGs) are the major component of plant storage lipid. Acyl-CoA:diacylglycerol acyltransferase (DGAT), 国際会議Vernonia DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s
ポスター発表
- 農芸化学会関西支部会, 2017年, 日本語, 国内会議ソルガム高活性型Rubiscoの小サブユニットを利用したイネの光合成能力の改良
口頭発表（一般）
- 農芸化学会関西支部会, 2017年, 日本語, 国内会議イネにおけるRubisco activase様タンパク質OsRca2の機能解析
口頭発表（一般）
- 第37回種子生理生化学研究会年会, 2016年11月, 日本語, 南あわじロイヤルホテル（兵庫県南あわじ市）, 国内会議土壌窒素レベルの変動がダイズ(Glycine max.L) 種子貯蔵物質含有率に与える影響
口頭発表（一般）
- 第29回植物脂質シンポジウム, 2016年11月, 日本語, 国内会議ベルノニアDGAT1遺伝子導入による高等植物の貯蔵脂質合成能の改良
ポスター発表
- 日本作物学会第241回講演会, 2016年03月, 日本語, 国内会議高等植物の貯蔵脂質合成の強化に関する研究
ポスター発表
- 日本作物学会講演会, 2016年03月, 日本語, 国内会議高CO2条件で育成したソルガムRbcS高発現・イネRbcS発現抑制2重形質転換イネの生理特性
ポスター発表
- 日本作物学会講演会, 2016年03月, 日本語, 国内会議デンプン合成関連酵素の発現制御に働くCRCTは葉鞘のデンプンの鎖長分布パターンに影響を与える
口頭発表（一般）
- 日本作物学会講演会, 2016年03月, 日本語, 国内会議シロイヌナズナにおけるCRCTオーソログの発現解析
ポスター発表
- 日本作物学会講演会, 2016年03月, 日本語, 国内会議イネ葉身の発達・老化過程におけるOsRca2 含量の変化
ポスター発表
- 日本作物学会講演会, 2016年03月, 日本語, 国内会議OsRbcS1の代謝機能：植物におけるOsRbcS1様RbcSの発現解析とOsRbcS1発現抑制イネのメタボローム解析
ポスター発表
- 日本植物生理学会年会, 2016年03月, 日本語, 国内会議CO2 Responsive CCT protein alters the chain-length distribution of starch from leaf sheath in rice by regulating the expression of multiple starch synthesis related enzymes
ポスター発表
- The 17th International Congress on Photosynthesis Research, 2016年, 英語, 国際会議Starch Content in Leaf Sheath Controlled by CO2 Responsive CCT Protein is a potential Determinant of Photosynthetic Capacity in Rice Grown under Elevated CO2 Condition
ポスター発表
- The 17th International Congress on Photosynthesis Research, 2016年, 英語, 国際会議Functional analysis of OsRbcS1, a non-photosynthetic small subunit that increases the catalytic turnover rate of Rubisco in rice
ポスター発表
- 日本植物生理学会年会, 2015年03月, 日本語, 国内会議Sink Capacity of Leaf Sheath Regulated by CRCT Affects the Photosynthetic Rate of Leaf Blade in Rice Grown under Elevated CO2 Condition.
口頭発表（一般）
- 日本植物生理学会年会, 2015年03月, 日本語, 国内会議Responsive CCT Protein Ortholog in Arabidopsis Controls the Starch Accumulation in Leaves.
ポスター発表
- 日本植物生理学会年会, 2015年03月, 日本語, 国内会議Functional analysis of Rubisco activase-like protein, OsRca2 in rice.
口頭発表（一般）
- 日本植物生理学会年会, 2015年03月, 日本語, 国内会議CO2 Responsive CCT Protein, CRCT Is a Positive Regulator of Starch Synthesis in Vegetative Organ in Rice.
ポスター発表
- 日本作物学会講演会, 2015年, 日本語, 国内会議Rubisco activase様タンパク質OsRca2の光合成における機能
口頭発表（一般）
- 日本作物学会, 2014年09月, 日本語, 愛媛大学, 国内会議耐塩性インディカイネ交雑由来倍加半数体系統の継続的耐塩性評価
口頭発表（一般）
- 日本作物学会講演会, 2014年09月, 日本語, 国内会議デンプン合成の制御因子CRCTの発現量がイネの生育と光合成に与える影響
ポスター発表
- 日本作物学会講演会, 2014年09月, 日本語, 国内会議イネ高活性型Rubisco小サブユニット遺伝子OsRbcS1のオーソログの発現解析
ポスター発表
- 第177回 近畿作物・育種研究会例会, 2014年07月, 日本語, 神戸大学農学部, 国内会議ダイズ体細胞胚長期懸濁培養におけるマルトースの効果に関する一考察
口頭発表（一般）
- 日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議高CO2条件下で発現促進されるCCTタンパク質CRCTはイネの栄養器官におけるデンプン合成の調節因子である
口頭発表（一般）
- 日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議寒地型イネ科牧草チモシーのRbcSを高発現する形質転換イネの光合成特性．
口頭発表（一般）
- 日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議ソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法でRubisco含量を減少させた二重形質転換イネの光合成特性．
口頭発表（一般）
- 日本作物学会, 2014年03月, 日本語, 千葉大学, 国内会議イネ高活性型Rubisco小サブユニットOsRbcS1の機能解析
口頭発表（一般）
- Plant Biology Meeting 2013, 2013年07月, 英語, American Society of Plant Biologists, 国際会議Increased oil and protein content in Glycine max.
ポスター発表
- 日本作物学会, 2013年, 日本語, 国内会議耐塩性イネ品種PokkaliのF1倍加半数体系統群における幼苗期耐塩性指標の評価
口頭発表（一般）
- 日本作物学会, 2013年, 日本語, 国内会議高CO2条件で発現促進される新規CCTドメインタンパク質CRCTは分げつ角度と基本代謝酵素の発現調節に働く
口頭発表（一般）
- 日本作物学会, 2013年, 日本語, 国内会議ソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法等でRubisco量を減少させた形質転換イネの生理特性
口頭発表（一般）
- 日本作物学会, 2013年, 日本語, 国内会議イネにおいて葉身で発現しない新規Rubisco小サブユニットOsRbcS1の機能解析
口頭発表（一般）
- 日本作物学会, 2013年, 日本語, 国内会議Rubisco activaseはRubisco生合成を阻害することによりRubiscoの発現を抑制する
口頭発表（一般）
- 日本作物学会, 2012年, 日本語, 国内会議幼苗期におけるインディカイネの交雑親と倍加半数体系統群の耐塩性比較
口頭発表（一般）
- 日本作物学会, 2012年, 日本語, 国内会議高CO2条件で発現促進される受容体型キナーゼOsTPK1の機能解析
口頭発表（一般）
- 日本植物生理学会, 2012年, 日本語, 国内会議イネにおいて高CO2条件下で発現促進される新規CCTタンパク質OsCCT1の機能解析
口頭発表（一般）
- 日本作物学会, 2011年, 日本語, 国内会議新規なCCTドメインタンパク質OsCCT1は高CO2環境への代謝的・形態的応答に関係している．
口頭発表（一般）
- 日本作物学会, 2011年, 日本語, 国内会議イネにおける葉緑体型ホスホグリセリン酸ムターゼOsPGMd2の機能解析
口頭発表（一般）
- 日本植物生理学会, 2011年, 日本語, 国内会議Rubisco activaseの高発現はRubisco含量の減少により光合成速度を低下させる
口頭発表（一般）
- 日本植物生理学会年会, 2010年, 日本語, 国内会議ソルガムRbcS の高発現は形質転換イネにおけるRubisco の反応回転速度を増加させる
口頭発表（一般）
- 日本作物学会, 2010年, 日本語, 国内会議イネにおけるプラスチド局在型エノラーゼ遺伝子の機能解析
口頭発表（一般）
- The 15th International Congress of Photosynthesis, 2010年, 英語, 国際会議Chimeric Incorporation of Sorghum RbcS Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice Plants
口頭発表（一般）
- 日本作物学会大会, 2009年, 日本語, 国内会議オオムギRubisco activaseの高発現がイネの光合成特性と葉内成分含量におよぼす効果
口頭発表（一般）
- 日本作物学会大会, 2008年, 日本語, 国内会議油料作物Jatropha curcasの組織培養による増殖と脂質定量
ポスター発表
- 近畿作物・育種研究会例会, 2008年, 日本語, 神戸大学, 国内会議油料作物Jatropha curcasの脂質含量と組織培養による増殖
口頭発表（一般）
- 日本作物学会大会, 2008年, 日本語, 国内会議丹波黒大豆‘兵系黒３号’の成熟培養における，糖とアミノ酸の効果
ポスター発表
- 近畿作物・育種研究会例会, 2008年, 日本語, 神戸大学, 国内会議開放系大気CO2 濃度増加条件（FACE)で育成したイネの遺伝子発現プロファイリング
口頭発表（一般）
- 日本作物学会大会, 2008年, 日本語, 国内会議イネの光合成能力改良に向けたイネ科植物内における高Kcat Rubiscoの探索
口頭発表（一般）
- he 1st International Symposium on Biocatalysis and Biotechnology, 2007年11月28日, 英語New research on the very promising biodiesel material, Jatropha crucas
[招待有り]
口頭発表（一般）
- 日本作物学会大会, 2007年, 日本語, 国内会議Ribulose-1,5-bisphosphate carboxylase/oxygenase activaseを過剰発現させた形質転換イネの光合成特性
口頭発表（一般）
- 日本農芸化学学会大会, 2006年, 日本語, 国内会議品種判別を目的とした丹波黒ダイズのSSR分析 – 系統樹作成と市販加工品の解析 -
口頭発表（一般）
- 日本作物学会大会, 2006年, 日本語, 国内会議Ribulose-1,5-bisphosphate carboxylase/oxygenase acitivase (Rubisco activase)含量を減少させた形質転換イネの光強度に対する光合成反応
ポスター発表
- 日本作物学会（日本作物学会紀事74巻第1号96-97）, 2005年, 日本語, 日本作物学会, 未記入, 国内会議野生イネOryza rufipogon由来の光合成能力関連QTLをもつ準同質遺伝子系統の光合成特性
ポスター発表
- The 4th International Crop Science Congress, 2004年10月, 英語, Association of International Crop Science, Brisbane, Australia, 国際会議Tanbaguro: a new model genotype of soybean for tissue culture study.
ポスター発表
- The 4th International Crop Science Congress, 2004年10月, 英語, Association of International Crop Science, Brisbane, Australia, 国際会議Photosynthesis, growth and yield of BC2F4 lines derived from Oryza sativa and wild rice species, O. rufipogon.
ポスター発表
- 95th AOCS Annual Meeting & Expo. P.25, 2004年05月10日, 英語, 未記入, 未記入, 国際会議Cloning of a soybean diacylglycerol acyltransferase (DGAT) cDNA and its expression in yeast in comparison to Vernonia DGAT.
口頭発表（一般）
- 日本食品科学工学会51回大会, 2004年, 日本語, 日本食品科学工学会, 岩手大学, 国内会議無機元素・アントシアニン分析による丹波黒（ダイズ）の産地判別
口頭発表（一般）
- 日本食品科学工学会大会, 2004年, 日本語, 国内会議無機元素・アントシアニン分析による丹波黒(ダイズ)の産地判別
口頭発表（一般）
- 日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 未記入, 国内会議丹波黒およびその加工品のSSR分析
口頭発表（一般）
- Soy 2004 10th Biennial Conference of the Cellular and Molecular Biology of the Soybean, p.144, 2004年, 英語, American Soybean Board, 未記入, 国際会議Seed oil of soybeans transformed with Stokesia epoxygenase have altered fatty acid profiles.
ポスター発表
- Industrial Applications of Renewable Resources, 2004, 2004年, 英語, Industrial Applications of Renewable Resources, 未記入, 国際会議Key genes involving in the selective accumulation of epoxy fatty acids in seed oil identified by comparative expression analysis.
ポスター発表
- 日本作物学会記事, 2003年, 日本語, 未記入, 未記入, 国内会議浮稲節間の伸長とエチレンによる蒸散の促進
口頭発表（一般）
- 日作紀, 2003年, 日本語, 未記入, 未記入, 国内会議SSR－PCR 法による丹波黒の判別（Ⅰ）
口頭発表（一般）
- The 15th International Symposium on Plant Lipids, 2002年05月14日, 英語Cloning and expression of Vernonia and Euphorbia diacylglycerol acyltransferase cDNAs.
ポスター発表