研究者紹介システム

吉田 健一
ヨシダ ケンイチ
大学院科学技術イノベーション研究科 科学技術イノベーション専攻
教授
自然科学関係
Last Updated :2020/06/04

研究者情報

所属

  • 【主配置】

    大学院科学技術イノベーション研究科 科学技術イノベーション専攻
  • 【配置】

    国際連携推進機構 EU総合学術センター, 大学院農学研究科 生命機能科学専攻, 先端バイオ工学研究センター

学位

  • 博士(農学), 京都大学

研究シーズ

授業科目

ジャンル

  • 科学・技術 / 生命科学

コメントテーマ

  • 遺伝子解析
  • イノシトール
  • 枯草菌
  • ゲノム生物学
  • 代謝工学

研究ニュース

研究活動

プロフィール情報

  • プロフィール

    After a Master obtained at Kyoto University in 1989, he got the position of Assistant professor at Fukuyama University in 1990 and obtained a PhD at Kyoto University in 1993. After a Post-Doc experience at INRA, France, from 1996 to 97, he moved to Kobe University in 2004 as Associate professor, and was promoted to be Professor of Applied Microbiology in 2009. He has specialized in functional genomics of bacteria including Bacillus subtilis and its relatives since the very beginning of his career to date. He was once awarded the prize for "Encouragement of Young Scientists" (2002) and twice the prize for "Excellent papers" from the Japan Society for Bioscience, Biotechnology, and Agrochemistry (2008 and 2014). He served in the Research Promotion Bureau in Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT), as a Program Officer (Scientific Research Senior Specialist) (2005-2007). Currently he serves as well in Kobe University Brussels European Centre as one of the directors (Executive director, since April 2014).

研究キーワード

  • イノシトール
  • 枯草菌
  • 根粒菌
  • Geobacillus
  • 代謝工学
  • 合成生物学

研究分野

  • ものづくり技術(機械・電気電子・化学工学) / バイオ機能応用、バイオプロセス工学
  • ライフサイエンス / ゲノム生物学
  • ライフサイエンス / 応用微生物学

受賞

  • 2019年01月 欧州微生物学会連合 Honorary FEMS Ambassador

    吉田 健一

  • 2014年03月 日本農芸化学会 日本農芸化学会論文賞2013 Award for Excellence to Authors Publishing in Bioscience, Biotechnology, and Biochemistry in 2013

    吉田 健一

    国内学会・会議・シンポジウム等の賞

  • 2009年03月 日本農芸化学会 日本農芸化学会論文賞2008 Award for Excellence to Authors Publishing in Bioscience, Biotechnology, and Biochemistry in 2008

    吉田 健一

    国内学会・会議・シンポジウム等の賞

  • 2009年03月 日本農芸化学会 日本農芸化学会英文誌Bioscience, Biotechnology, and Biochemistry論文賞 Identification of Two Major Ammonia-Releasing Reactions Involved in Secondary Natto Fermentation

    SIGEKI KADA;MASAHIRO YABUSAKI;TAKAYUKI KAGA;ASHIDA HITOSHI;KEN-ICHI YOSHIDA

    日本国

    学会誌・学術雑誌による顕彰

  • 2003年03月 日本農芸化学会 農芸化学奨励賞 ゲノム情報に基づく枯草菌の逆遺伝学的研究

    吉田 健一

論文

  • Christophe Michon, Choong-Min Kang, Sophia Karpenko, Kosei Tanaka, Shu Ishikawa, Ken-Ichi Yoshida

    A rare stereoisomer of inositol, scyllo-inositol, is a therapeutic agent that has shown potential efficacy in preventing Alzheimer's disease. Mycobacterium tuberculosis ino1 encoding myo-inositol-1-phosphate (MI1P) synthase (MI1PS) was introduced into Bacillus subtilis to convert glucose-6-phosphate (G6P) into MI1P. We found that inactivation of pbuE elevated intracellular concentrations of NAD+·NADH as an essential cofactor of MI1PS and was required to activate MI1PS. MI1P thus produced was dephosphorylated into myo-inositol by an intrinsic inositol monophosphatase, YktC, which was subsequently isomerized into scyllo-inositol via a previously established artificial pathway involving two inositol dehydrogenases, IolG and IolW. In addition, both glcP and glcK were overexpressed to feed more G6P and accelerate scyllo-inositol production. Consequently, a B. subtilis cell factory was demonstrated to produce 2 g L-1 scyllo-inositol from 20 g L-1 glucose. This cell factory provides an inexpensive way to produce scyllo-inositol, which will help us to challenge the growing problem of Alzheimer's disease in our aging society.

    2020年03月02日, Communications biology, 3 (1), 93 - 93, 英語, 国際誌

    [査読有り]

  • Yoshida, Ken-ichi, Ishikawa, Shu

    2019年, J Nutr Sci Vitaminol, 65, S139 - S142, 英語

    [査読有り]

  • Lim, L, Senba, H, Kimura, Y, Yokota, S, Doi, M, Yoshida, K, Takenaka, S

    2019年, Process Biochem, 79, 74 - 80, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nishihata Shogo, Kondo Takahiko, Tanaka Kosei, Ishikawa Shu, Takenaka Shinji, Kang Choong-Min, Yoshida Ken-ichi

    Background Bradyrhizobium diazoefficiens USDA110 nodulates soybeans for nitrogen fixation. It accumulates poly-3-hydroxybutyrate (PHB), which is of physiological importance as a carbon/energy source for survival during starvation, infection, and nitrogen fixation conditions. PHB accumulation is orchestrated by not only the enzymes for PHB synthesis but also PHB-binding phasin p

    BMC, 2018年10月, BMC Microbiology, 18, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Miyano Megumi, Tanaka Kosei, Ishikawa Shu, Mori Kotaro, Miguel-Arribas Andres, Meijer Wilfried J. J, Yoshida Ken-ichi

    Background: Bacterial strains of the genus Geobacillus grow at high temperatures of 50-75 °C and could thus be useful for biotechnological applications. However, genetic manipulation of these species is difficult because the current techniques for transforming Geobacillus species are not efficient. In this study, we developed an easy and efficient method for transforming Geobac

    BMC, 2018年08月, Microbial Cell Factories, 17, 英語

    [査読有り]

    研究論文(学術雑誌)

  • UEDA-WAKAGI Manabu, HAYASHIBARA Kaori, NAGANO Tomoya, IKEDA Masaki, YUAN Sihao, UEDA Shuji, SHIRAI Yasuhito, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    2018年, Food and Function, 9, 4223 - 4233, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Miyano Megumi, Tanaka Kosei, Ishikawa Shu, Takenaka Shinji, Miguel-Arribas Andres, Meijer Wilfried J. J, Yoshida Ken-ichi

    Background: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. Results: In this study, the oriT sequence of pLS20cat (oriT(LS20)) was eliminated to obtain the plasmid, pLS20catΔ

    BioMed Central, 2018年01月, Microbial Cell Factories, 17, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naoko Okai, Takaya Masuda, Yasunobu Takeshima, Kosei Tanaka, Ken-Ichi Yoshida, Masanori Miyamoto, Chiaki Ogino, Akihiko Kondo

    Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.

    2017年12月, AMB Express, 7 (1), 130 - 130, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Takahiro Ozeki, Kosei Tanaka, Ken-ichi Yoshida

    To predict the amino acid residues playing important roles in acetyl-CoA and substrate binding and to study the acetyl group transfer mechanism of Chryseobacterium sp. 5-3B N-acetyltransferase (5-3B NatA). A 3-dimensional homology model of 5-3B NatA was constructed to compare the theoretical structure of this compound with the structures of previously reported proteins belonging to the bacterial GCN5 N-acetyltransferase family. Homology modeling of the 5-3B NatA structure and a characterization of the enzyme's kinetic parameters identified the essential amino acid residues involved in binding and acetyl-group transfer. (126)Leu, (132)Leu, and (135)Lys were implicated in the binding of phosphopantothenic acid, and (100)Tyr and (131)Lys in that of adenosyl biphosphate. The data supported the participation of (83)Glu and (133)Tyr in catalyzing acetyl-group transfer to l-2-phenylglycine. 5-3B NatA catalyzes the enantioselective N-acetylation of l-2-phenylglycine via a ternary complex comprising the enzyme, acetyl-CoA, and the substrate.

    SPRINGER, 2017年11月, BIOTECHNOLOGY LETTERS, 39 (11), 1699 - 1707, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Dong-Min Kang, Christophe Michon, Tetsuro Morinaga, Kosei Tanaka, Shinji Takenaka, Shu Ishikawa, Ken-ichi Yoshida

    Background: Bacillus subtilis is able to utilize at least three inositol stereoisomers as carbon sources, myo-, scyllo-, and D-chiro-inositol (MI, SI, and DCI, respectively). NAD(+)-dependent SI dehydrogenase responsible for SI catabolism is encoded by iolX. Even in the absence of functional iolX, the presence of SI or MI in the growth medium was found to induce the transcription of iolX through an unknown mechanism. Results: Immediately upstream of iolX, there is an operon that encodes two genes, yisR and iolQ (formerly known as degA), each of which could encode a transcriptional regulator. Here we performed an inactivation analysis of yisR and iolQ and found that iolQ encodes a repressor of the iolX transcription. The coding sequence of iolQ was expressed in Escherichia coli and the gene product was purified as a His-tagged fusion protein, which bound to two sites within the iolX promoter region in vitro. Conclusions: IolQ is a transcriptional repressor of iolX. Genetic evidences allowed us to speculate that SI and MI might possibly be the intracellular inducers, however they failed to antagonize DNA binding of IolQ in in vitro experiments.

    BIOMED CENTRAL LTD, 2017年07月, BMC MICROBIOLOGY, 17 (1), 154, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Dong-Min Kang, Kosei Tanaka, Shinji Takenaka, Shu Ishikawa, Ken-ichi Yoshida

    Bacillus subtilis genes iolG, iolW, iolX, ntdC, yfiI, yrbE, yteT, and yulF belong to the Gfo/Idh/MocA family. The functions of iolG, iolW, iolX, and ntdC are known; however, the functions of the others are unknown. We previously reported the B. subtilis cell factory simultaneously overexpressing iolG and iolW to achieve bioconversion of myo-inositol (MI) into scyllo-inositol (SI). YulF shares a significant similarity with IolW, the NADP(+)-dependent SI dehydrogenase. Transcriptional abundance of yulF did not correlate to that of iol genes involved in inositol metabolism. However, when yulF was overexpressed instead of iolW in the B. subtilis cell factory, SI was produced from MI, suggesting a similar function to iolW. In addition, we demonstrated that recombinant His(6)-tagged YulF converted scyllo-inosose into SI in an NADPH-dependent manner. We have thus identified yulF encoding an additional NADP(+)-dependent SI dehydrogenase, which we propose to rename iolU.

    TAYLOR & FRANCIS LTD, 2017年05月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81 (5), 1026 - 1032, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Genome Sequence of Bacillus velezensis S141, a New Strain of Plant Growth-Promoting Rhizobacterium Isolated from Soybean Rhizosphere.

    Sibponkrung, S, Kondo, T, Tanaka, K, Tittabutr, P, Boonkerd, N, Teaumroong, N, Yoshida, K

    2017年05月, Genome Announc., 5 (48), 英語

    [査読有り]

  • Kosei Tanaka, Ayane Natsume, Shu Ishikawa, Shinji Takenaka, Ken-ichi Yoshida

    Background: A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inositol stereoisomers, including MI and SI. Previously, we reported a B. subtilis cell factory with modified inositol metabolism that converts MI into SI in the culture medium. The strain was constructed by deleting all genes related to inositol metabolism and overexpressing key enzymes, IolG and IolW. By using this strain, 10 g/l of MI initially included in the medium was completely converted into SI within 48 h of cultivation in a rich medium containing 2% (w/v) Bacto soytone. Results: When the initial concentration of MI was increased to 50 g/l, conversion was limited to 15.1g/l of SI. Therefore, overexpression systems of IolT and PntAB, the main transporter of MI in B. subtilis and the membrane-integral nicotinamide nucleotide transhydrogenase in Escherichia coli respectively, were additionally introduced into the B. subtilis cell factory, but the conversion efficiency hardly improved. We systematically determined the amount of Bacto soytone necessary for ultimate conversion, which was 4% (w/v). As a result, the conversion of SI reached to 27.6 g/l within 48 h of cultivation. Conclusions: The B. subtilis cell factory was improved to yield a SI production rate of 27.6 g/l/48 h by simultaneous overexpression of IolT and PntAB, and by addition of 4% (w/v) Bacto soytone in the conversion medium. The concentration of SI was increased even in the stationary phase perhaps due to nutrients in the Bacto soytone that contribute to the conversion process. Thus, MI conversion to SI may be further optimized via identification and control of these unknown nutrients.

    BIOMED CENTRAL LTD, 2017年04月, MICROBIAL CELL FACTORIES, 16, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Mayo Umeda, Hisanori Senba, Dai Koyama, Kosei Tanaka, Ken-ichi Yoshida, Mikiharu Doi

    BACKGROUNDAspergillus repens strain MK82 produces an aspartic protease (PepA_MK82) that efficiently decolorises red-pigmented proteins during dried bonito fermentation. However, further expansion of the industrial applications of PepA_MK82 requires the high-level production and efficient preparation of the recombinant enzyme. RESULTSThe genomic DNA and cDNA fragments encoding the protease were cloned from strain MK82 and sequenced. Phylogenetic analysis of PepA_MK82 and comparisons with previously reported fungal aspartic proteases showed that PepA_MK 82 clusters with different groups of these enzymes. Heterologous expression of PepA_MK82 in Pichia pastoris yielded preparations of higher purity than obtained with an Escherichia coli expression system. Total protease activity in a 100-mL culture of the P. pastoris transformant was 14 times higher than that from an equivalent culture of A. repenseMK82. The recombinant PepA_MK82 was easily obtained via acetone precipitation; the final recovery was 83%. PepA_MK82 and its recombinant had similar characteristics in terms of their optimal pH, thermostability, and decolorisation activity. The recombinant was also able to decolorise flaked, dried bonito and to bleach a blood-stained cloth. CONCLUSIONGiven its ability to hydrolyse and decolorise red-pigmented proteins, recombinant PepA_MK8 can be exploited in the food industry and as a stain-removal agent in laundry applications. (c) 2016 Society of Chemical Industry

    WILEY-BLACKWELL, 2017年01月, JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 97 (1), 95 - 101, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kengo Sasaki, Daisuke Sasaki, Naoko Okai, Kosei Tanaka, Ryohei Nomoto, Itsuko Fukuda, Ken-Ichi Yoshida, Akihiko Kondo, Ro Osawa

    Accumulating evidence suggests that dietary taurine (2-aminoethanesulfonic acid) exerts beneficial anti-inflammatory effects in the large intestine. In this study, we investigated the possible impact of taurine on human colonic microbiota using our single-batch fermentation system (Kobe University Human Intestinal Microbiota Model; KUHIMM). Fecal samples from eight humans were individually cultivated with and without taurine in the KUHIMM. The results showed that taurine remained largely undegraded after 30 h of culturing in the absence of oxygen, although some 83% of the taurine was degraded after 30 h of culturing under aerobic conditions. Diversity in bacterial species in the cultures was analyzed by 16S rRNA gene sequencing, revealing that taurine caused no significant change in the diversity of the microbiota; both operational taxonomic unit and Shannon-Wiener index of the cultures were comparable to those of the respective source fecal samples. In addition, principal coordinate analysis indicated that taurine did not alter the composition of bacterial species, since the 16S rRNA gene profile of bacterial species in the original fecal sample was maintained in each of the cultures with and without taurine. Furthermore, metabolomic analysis revealed that taurine did not affect the composition of short-chain fatty acids produced in the cultures. These results, under these controlled but artificial conditions, suggested that the beneficial anti-inflammatory effects of dietary taurine in the large intestine are independent of the intestinal microbiota. We infer that dietary taurine may act directly in the large intestine to exert anti-inflammatory effects.

    2017年, PloS one, 12 (7), e0180991, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Ayako Terakawa, Ayane Natsume, Atsushi Okada, Shogo Nishihata, Junko Kuse, Kosei Tanaka, Shinji Takenaka, Shu Ishikawa, Ken-ichi Yoshida

    Background: In Escherichia coli, nagD, yrfG, yjjG, yieH, yigL, surE, and yfbR encode 5'-nucleotidases that hydrolyze the phosphate group of 5'-nucleotides. In Bacillus subtilis, genes encoding 5'-nucleotidase have remained to be identified. Results: We found that B. subtilis ycsE, araL, yutF, ysaA, and yqeG show suggestive similarities to nagD. Here, we expressed them in E. coli to purify the respective His(6)-tagged proteins. YcsE exhibited significant 5'-nucleotidase activity with a broader specificity, whereas the other four enzymes had rather weak but suggestive activities with various capacities and substrate specificities. In contrast, B. subtilis yktC shares high similarity with E. coli suhB encoding an inositol monophosphatase. YktC exhibited inositol monophosphatase activity as well as 5'-nucleotidase activity preferential for GMP and IMP. The ycsE, yktC, and yqeG genes are induced by oxidative stress and were dispensable, although yqeG was required to maintain normal growth on solid medium. In the presence of diamide, only mutants lacking yktC exhibited enhanced growth defects, whereas the other mutants without ycsE or yqeG did not. Conclusions: Accordingly, in B. subtilis, at least YcsE and YktC acted as major 5'-nucleotidases and the four minor enzymes might function when the intracellular concentrations of substrates are sufficiently high. In addition, YktC is involved in resistance to oxidative stress caused by diamide, while YqeG is necessary for normal colony formation on solid medium.

    BIOMED CENTRAL LTD, 2016年10月, BMC Microbiology, 16 (1), 1 - 13, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Risa Takagi, Kengo Sasaki, Daisuke Sasaki, Itsuko Fukuda, Kosei Tanaka, Ken-ichi Yoshida, Akihiko Kondo, Ro Osawa

    We devised a single-batch fermentation system to simulate human colonic microbiota from fecal samples, enabling the complex mixture of microorganisms to achieve densities of up to 1011 cells/mL in 24 h. 16S rRNA gene sequence analysis of bacteria grown in the system revealed that representatives of the major phyla, including Bacteroidetes, Firmicutes, and Actinobacteria, as well as overall species diversity, were consistent with those of the original feces. On the earlier stages of fermentation (up to 9 h), trace mixtures of acetate, lactate, and succinate were detectable; on the later stages (after 24 h), larger amounts of acetate accumulated along with some of propionate and butyrate. These patterns were similar to those observed in the original feces. Thus, this system could serve as a simple model to simulate the diversity as well as the metabolism of human colonic microbiota. Supplementation of the system with several prebiotic oligosaccharides (including fructo-, galacto-, isomalto-, and xylo-oligosaccharides; lactulose; and lactosucrose) resulted in an increased population in genus Bifidobacterium, concomitant with significant increases in acetate production. The results suggested that this fermentation system may be useful for in vitro, preclinical evaluation of the effects of prebiotics prior to testing in humans.

    PUBLIC LIBRARY SCIENCE, 2016年08月, PLOS ONE, 11 (8), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Parastoo Majidian, Junko Kuse, Kosei Tanaka, Hamid Najafi, Mehrshad Zeinalabedini, Shinji Takenaka, Ken-ichi Yoshida

    We modified GntR regulation in Bacillus subtilis to devise transient induction systems. GntR is the repressor antagonized by gluconate to induce transcription of the gntRKPZ operon for gluconate catabolism. On the other hand, the gnt operon is repressed by glucose via carbon catabolite repression involving CcpA/P-ser-HPr, which binds to two cre sites: one located in the gnt promoter region and the other within the gntR coding region. We initiated gntKPZ encoding of enzymes for gluconate catabolism expressed independently from the operon; this allowed constitutive degradation of gluconate. Both cre sites were mutated to abolish catabolite repression. The mutated gnt promoter was set up to drive the expression of the lacZ reporter under the control of GntR. Even in the presence of glucose, lacZ was induced upon the addition of gluconate and shut down again as gluconate was consumed. Thus, modified GntR regulation enables artificial transient induction. This will allow us to design a flexible metabolic engineering system with genes expressed only temporarily as desired.

    MICROBIOL RES FOUNDATION, 2016年, JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 62 (6), 277 - 285, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tsuji S, Tanaka K, Takenaka S, Yoshida KI

    2015年05月, Bioscience, biotechnology, and biochemistry, 79 (11), 1906 - 1914

    [査読有り]

  • Itsuko Fukuda, Shin Nishiumi, Rie Mukai, Ken-ichi Yoshida, Hitoshi Ashida

    Polycyclic aromatic hydrocarbons (PAHs) and halogenated aromatic hydrocarbons (HAHs) develop various adverse effects through activation of an aryl hydrocarbon receptor (AhR). The suppressive effects of brewed green tea and black tea on 3-methylcholanthrene (MC)-induced AhR activation and its downstream events were examined in the liver of rats. Ad-libitum drinking of green tea and black tea suppressed MC-induced AhR activation and elevation of ethoxyresorufin O-deethylase activity in the liver, whereas the teas themselves did not induce them. Tea showed a suppressive fashion on the expression of cytochrome P450 1A1 (CYP1A1). Tea suppressed the AhR activation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) ex vivo. A part of catechins and theaflavins was present in plasma and liver as conjugated and intact forms. The results of this study suggested that active component(s) of tea are incorporated in the liver and suppress the activity of CYP1As through the AhR activation pathway.

    INFORMA HEALTHCARE, 2015年05月, INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 66 (3), 300 - 307, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Suzuki H, Ishii J, Kondo A, Yoshida K

    2015年02月, Biotechnology letters, 37 (2), 429 - 435

    [査読有り]

  • Tanaka K, Iwasaki K, Morimoto T, Matsuse T, Hasunuma T, Takenaka S, Chumsakul O, Ishikawa S, Ogasawara N, Yoshida K

    2015年02月, BMC microbiology, 15 (1)

    [査読有り]

  • scyllo-Inositol, a Therapeutic Agent for Alzheimer’s Disease

    吉田 健一

    2015年, Austin J Clin Neurol, 2 (4), 1040, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takenaka S, Miyatake A, Tanaka K, Kuntiya A, Techapun C, Leksawasdi N, Seesuriyachan P, Chaiyaso T, Watanabe M, Yoshida K

    2015年, J Basic Microbiol, 55 (6), 780 - 789, 英語

    [査読有り]

    研究論文(学術雑誌)

  • FUKUDA Itsuko, NISHIUMI Shin, MUKAI Rie, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    2015年, International Journal of Food Sciences and Nutrition, 66, 300 - 307, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Sanchez-Vizuete P, Tanaka K, Bridier A, Shirae Y, Yoshida K, Bouchez T, Aymerich S, Briandet R, Le Coq D

    2014年09月, Genome announcements, 2 (5)

    [査読有り]

  • Tanaka K, Takanaka S, Yoshida K

    2014年09月, Bioengineered, 5 (5), 331 - 334

    [査読有り]

  • Poly-β-hydroxybutyrate accumulation in Bradyrhizobium japonicum depends on proteins referred to as phasins

    吉田 健一, 田中 耕生

    Wageningen Academic Publishers, 2014年05月, Industrial, medical and environmental applications of microorganisms. Current status and trends, 470 - 475, 英語

    [査読有り][招待有り]

    研究論文(国際会議プロシーディングス)

  • A Bacillus subtilis cell factory for producing scyllo-inositol

    吉田 健一, 田中 耕生

    Wageningen Academic Publishers, 2014年05月, Industrial, medical and environmental applications of microorganisms. Current status and trends, 636 - 640, 英語

    [査読有り][招待有り]

    研究論文(国際会議プロシーディングス)

  • Shuhei Ueda, Ryohei Nomoto, Ken-ichi Yoshida, Ro Osawa

    Background: Tannase (tannin acyl hydrolase, EC 3.1.1.20) specifically catalyzes the hydrolysis of the galloyl ester bonds in hydrolyzable tannins to release gallic acid. The enzyme was found not only in fungal species but also many bacterial species including Lactobacillus plantarum, L. paraplantarum, and L. pentosus. Recently, we identified and expressed a tannase gene of L. plantarum, tanLpl, to show remarkable differences to characterized fungal tannases. However, little is known about genes responsible for tannase activities of L. paraplantarum and L. pentosus. We here identify the tannase genes (i.e. tanLpa and tanLpe) of the above lactobacilli species, and describe their molecular diversity among the strains as well as enzymological difference between species inclusive of L. plantarum. Results: The genes encoding tannase, designated tanLpa and tanLpe, were cloned from Lactobacillus paraplantarum NSO120 and Lactobacillus pentosus 21A-3, which shared 88% and 72% amino acid identity with TanLpl, cloned from Lactobacillus plantarum ATCC 14917(T), respectively. These three enzymes could comprise a novel tannase subfamily of independent lineage, because no other tannases in the databases share significant sequence similarity with them. Each of tanLpl, tanLpa, and tanLpe was expressed in Bacillus subtilis RIK 1285 and recombinant enzymes were secreted and purified. The K-m values of the enzymes on each galloyl ester were comparable; however, the k(cat)/K-m values of TanLpa for EGCg, ECg, Cg, and GCg were markedly higher than those for TanLpl and TanLpe. Their enzymological properties were compared to reveal differences at least in substrate specificity. Conclusion: Two tannase genes responsible for tannase activities of L. paraplantarum and L. pentosus were identified and characterized. TanLpl, TanLpa and TanLpe forming a phylogenetic cluster in the known bacterial tannase genes and had a limited diversity in each other. Their enzymological properties were compared to reveal differences at least in substrate specificity. This is the first comparative study of closely related bacterial tannases.

    BIOMED CENTRAL LTD, 2014年04月, BMC MICROBIOLOGY, 14 (1), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shuhei Ueda, Ryohei Nomoto, Ken-ichi Yoshida, Ro Osawa

    Background: Tannase (tannin acyl hydrolase, EC 3.1.1.20) specifically catalyzes the hydrolysis of the galloyl ester bonds in hydrolyzable tannins to release gallic acid. The enzyme was found not only in fungal species but also many bacterial species including Lactobacillus plantarum, L. paraplantarum, and L. pentosus. Recently, we identified and expressed a tannase gene of L. plantarum, tanLpl, to show remarkable differences to characterized fungal tannases. However, little is known about genes responsible for tannase activities of L. paraplantarum and L. pentosus. We here identify the tannase genes (i.e. tanLpa and tanLpe) of the above lactobacilli species, and describe their molecular diversity among the strains as well as enzymological difference between species inclusive of L. plantarum. Results: The genes encoding tannase, designated tanLpa and tanLpe, were cloned from Lactobacillus paraplantarum NSO120 and Lactobacillus pentosus 21A-3, which shared 88% and 72% amino acid identity with TanLpl, cloned from Lactobacillus plantarum ATCC 14917(T), respectively. These three enzymes could comprise a novel tannase subfamily of independent lineage, because no other tannases in the databases share significant sequence similarity with them. Each of tanLpl, tanLpa, and tanLpe was expressed in Bacillus subtilis RIK 1285 and recombinant enzymes were secreted and purified. The K-m values of the enzymes on each galloyl ester were comparable; however, the k(cat)/K-m values of TanLpa for EGCg, ECg, Cg, and GCg were markedly higher than those for TanLpl and TanLpe. Their enzymological properties were compared to reveal differences at least in substrate specificity. Conclusion: Two tannase genes responsible for tannase activities of L. paraplantarum and L. pentosus were identified and characterized. TanLpl, TanLpa and TanLpe forming a phylogenetic cluster in the known bacterial tannase genes and had a limited diversity in each other. Their enzymological properties were compared to reveal differences at least in substrate specificity. This is the first comparative study of closely related bacterial tannases.

    BIOMED CENTRAL LTD, 2014年04月, BMC MICROBIOLOGY, 14, 87, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Kenji Yoshida, Kosei Tanaka, Ken-ichi Yoshida

    N-Acetyltransferase from Chryseobacterium sp. strain 5-3B is an acetyl coenzyme A (acetyl-CoA)-dependent enzyme that catalyzes the enantioselective transfer of an acetyl group from acetyl-CoA to the amino group of L-2-phenylglycine to produce (2S)-2-acetylamino-2-phenylacetic acid. We purified the enzyme from strain 5-3B and deduced the N-terminal amino acid sequence. The gene, designated natA, was cloned with two other hypothetical protein genes; the three genes probably form a 2.5-kb operon. The deduced amino acid sequence of NatA showed high levels of identity to sequences of putative N-acetyltransferases of Chryseobacterium spp. but not to other known arylamine and arylalkylamine N-acetyltransferases. Phylogenetic analysis indicated that NatA forms a distinct lineage from known N-acetyltransferases. We heterologously expressed recombinant NatA (rNatA) in Escherichia coli and purified it. rNatA showed high activity for L-2-phenylglycine and its chloro-and hydroxyl-derivatives. The K-m and V-max values for L-2-phenylglycine were 0.145 +/- 0.026 mM and 43.6 +/- 2.39 mu mol . min(-1) . mg protein(-1), respectively. The enzyme showed low activity for 5-aminosalicylic acid and 5-hydroxytryptamine, which are reported as good substrates of a known arylamine N-acetyltransferase and an arylalkylamine N-acetyltransferase. rNatA had a comparatively broad acyl donor specificity, transferring acyl groups to L-2-phenylglycine and producing the corresponding 2-acetylamino-2-phenylacetic acids (relative activity with acetyl donors acetyl-CoA, propanoyl-CoA, butanoyl-CoA, pentanoyl-CoA, and hexanoyl-CoA, 100:108:122:10:<1).

    AMER SOC MICROBIOLOGY, 2014年03月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80 (5), 1770 - 1776, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Kenji Yoshida, Kosei Tanaka, Ken-ichi Yoshida

    N-Acetyltransferase from Chryseobacterium sp. strain 5-3B is an acetyl coenzyme A (acetyl-CoA)-dependent enzyme that catalyzes the enantioselective transfer of an acetyl group from acetyl-CoA to the amino group of L-2-phenylglycine to produce (2S)-2-acetylamino-2-phenylacetic acid. We purified the enzyme from strain 5-3B and deduced the N-terminal amino acid sequence. The gene, designated natA, was cloned with two other hypothetical protein genes; the three genes probably form a 2.5-kb operon. The deduced amino acid sequence of NatA showed high levels of identity to sequences of putative N-acetyltransferases of Chryseobacterium spp. but not to other known arylamine and arylalkylamine N-acetyltransferases. Phylogenetic analysis indicated that NatA forms a distinct lineage from known N-acetyltransferases. We heterologously expressed recombinant NatA (rNatA) in Escherichia coli and purified it. rNatA showed high activity for L-2-phenylglycine and its chloro-and hydroxyl-derivatives. The K-m and V-max values for L-2-phenylglycine were 0.145 +/- 0.026 mM and 43.6 +/- 2.39 mu mol . min(-1) . mg protein(-1), respectively. The enzyme showed low activity for 5-aminosalicylic acid and 5-hydroxytryptamine, which are reported as good substrates of a known arylamine N-acetyltransferase and an arylalkylamine N-acetyltransferase. rNatA had a comparatively broad acyl donor specificity, transferring acyl groups to L-2-phenylglycine and producing the corresponding 2-acetylamino-2-phenylacetic acids (relative activity with acetyl donors acetyl-CoA, propanoyl-CoA, butanoyl-CoA, pentanoyl-CoA, and hexanoyl-CoA, 100:108:122:10:<1).

    AMER SOC MICROBIOLOGY, 2014年03月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80 (5), 1770 - 1776, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Thi Lan Thanh Bien, Shogo Tsuji, Kosei Tanaka, Shinji Takenaka, Ken-ichi Yoshida

    Bacillus subtilis is used industrially for the production of secreted enzymes. The most characteristic feature of the secreted enzymes is variation in the N-terminal signal peptides that is recognized by secretion machinery, which is one of the determinants of efficiency and must be customized in each case. Culturing cellulolytic B. subtilis to secrete heterologous cellulases combined with customized signal peptides would be beneficial for producing biocommodities from cellulosic biomass. Four Clostridium thermocellum genes, encoding endoglucanases (celA and celB) and exoglucanases (celK and celS) were cloned to construct random libraries of combinations with 173 different signal peptides originating from the B. subtilis genome. The libraries were successfully screened to identify the signal peptides most efficient in secretion of each of the four cellulases, which were theoretically unpredictable. The secreted cellulases were assayed on carboxymethyl cellulose, phosphoric acid swollen cellulose, and microcrystalline cellulose to determine the possible effects of the signal peptides on substrate specificity. The customized signal peptides for CelA, CelB, and CelS did not affect enzyme performance but those for CelK might influence its substrate specificity.

    MICROBIOL RES FOUNDATION, 2014年, JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 60 (5), 175 - 182, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yoshida K, Takemoto Y, Sotsuka T, Tanaka K, Takenaka S

    Background: Bradyrhizobium japonicum USDA110, a soybean symbiont, is capable of accumulating a large amount of poly-beta-hydroxybutyrate (PHB) as an intracellular carbon storage polymer during free-living growth. Within the genome of USDA110, there are a number of genes annotated as paralogs of proteins involved in PHB metabolism, including its biosynthesis, degradation, and stabilization of its granules. They include two phbA paralogs encoding 3-ketoacyl-CoA thiolase, two phbB paralogs encoding acetoacetylCoA reductase, five phbC paralogs encoding PHB synthase, two phaZ paralogs encoding PHB depolymerase, at least four phaP phasin paralogs for stabilization of PHB granules, and one phaR encoding a putative transcriptional repressor to control phaP expression. Results: Quantitative reverse-transcriptase PCR analyses of RNA samples prepared from cells grown using three different media revealed that PHB accumulation was related neither to redundancy nor expression levels of the phbA, phbB, phbC, and phaZ paralogs for PHB-synthesis and degradation. On the other hand, at least three of the phaP paralogs, involved in the growth and stabilization of PHB granules, were induced under PHB accumulating conditions. Moreover, the most prominently induced phasin exhibited the highest affinity to PHB in vitro; it was able to displace PhaR previously bound to PHB. Conclusions: These results suggest that PHB accumulation in free-living B. japonicum USDA110 may not be achieved by controlling production and degradation of PHB. In contrast, it is achieved by stabilizing granules autonomously produced in an environment of excess carbon sources together with restricted nitrogen sources.

    BIOMED CENTRAL LTD, 2013年12月, BMC microbiology, 13 (1), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kosei Tanaka, Shintaro Tajima, Shinji Takenaka, Ken-ichi Yoshida

    Background: Bacillus subtilis 168 possesses an efficient pathway to metabolize some of the stereoisomers of inositol, including myo-inositol (MI) and scyllo-inositol (SI). Previously we reported a prototype of a B. subtilis cell factory with modified inositol metabolism that converts MI into SI in the culture medium. However, it wasted half of initial 1.0% (w/v) MI, and the conversion was limited to produce only 0.4% (w/v) SI. To achieve a more efficient SI production, we attempted additional modifications. Results: All "useless" genes involved in MI and SI metabolism were deleted. Although no elevation in SI production was observed in the deletion strain, it did result in no wastage of MI anymore. Thus additionally, overexpression of the key enzymes, IolG and IolW, was appended to demonstrate that simultaneous overexpression of them enabled complete conversion of all MI into SI. Conclusions: The B. subtilis cell factory was improved to yield an SI production rate of 10 g/L/48 h at least. The improved conversion was achieved only in the presence of enriched nutrition in the form of 2% (w/v) Bacto soytone in the medium, which may be due to the increasing demand for regeneration of cofactors.

    BIOMED CENTRAL LTD, 2013年12月, MICROBIAL CELL FACTORIES, 12 (1), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hirokazu Suzuki, Ken-ichi Yoshida, Toshihisa Ohshima

    Thermophiles have important advantages over mesophiles as host organisms for high-temperature bioprocesses, functional production of thermostable enzymes, and efficient expression of enzymatic activities in vivo. To capitalize on these advantages of thermophiles, we describe here a new inducible gene expression system in the thermophile Geobacillus kaustophilus HTA426. Six promoter regions in the HTA426 genome were identified and analyzed for expression profiles using beta-galactosidase reporter assay. This analysis identified a promoter region upstream of a putative amylose-metabolizing gene cluster that directed high-level expression of the reporter gene. The expression was >280-fold that without a promoter and was further enhanced 12-fold by maltose addition. In association with a multicopy plasmid, this promoter region was used to express heterologous genes. Several genes, including a gene whose product was insoluble when expressed in Escherichia coli, were successfully expressed as soluble proteins, with yields of 0.16 to 59 mg/liter, and conferred new functions to G. kaustophilus strains. Remarkably, cellulase and alpha-amylase genes conferred the ability to degrade cellulose paper and insoluble starch at high temperatures, respectively, generating thermophiles with the potential to degrade plant biomass. Our results demonstrate that this novel expression system expands the potential applications of G. kaustophilus.

    AMER SOC MICROBIOLOGY, 2013年09月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79 (17), 5151 - 5158, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 吉田 健一

    2013年08月, Biosci Biotechnol Biochem, 77 (8), 1709 - 1714, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Yuuta Honma, Kenji Yoshida, Ken-ichi Yoshida

    The demand for d-2-phenylglycine used to synthesize semisynthetic antibiotics and pesticides is increasing. We have isolated a Chryseobacterium sp. that selectively transformed the l-form of racemic d,l-2-phenylglycine to (2S)-2-acetylamide-2-phenylacetic acid with a molar yield of 50 % and an enantiomer excess of > 99.5 % under optimal culture conditions, consequently resulting in 99 % pure d-2-phenylglycine remaining in the culture. The enantioselective N-acetylation was catalyzed by an acetyl-CoA-dependent N-acetyltransferase whose synthesis was induced by l-2-phenylglycine. The enzyme differed from previously reported bacterial arylamine N-acetyltransferases in molecular mass and substrate specificity. The relative activity ratio of the enzyme with the substrates l-2-phenylglycine, d-2-phenylglycine, 2-(2-chlorophenyl)glycine, and 5-aminosalicylic acid (a good substrate of arylamine N-acetyltransferase) was 100:0:56.9:5.49, respectively. The biotransformation by the N-acetyltransferase-producing bacterium reported here could constitute a new preparative route for the enzymatic resolution of d,l-2-phenylglycine.

    SPRINGER, 2013年07月, BIOTECHNOLOGY LETTERS, 35 (7), 1053 - 1059, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yamashita Y, Yamaoka M, Hasunuma T, Ashida H, Yoshida K

    2013年05月, Journal of agricultural and food chemistry, 61 (20), 4850 - 4854

    [査読有り]

  • Kada S, Ishikawa A, Ohshima Y, Yoshida K

    Natto is a traditional Japanese food made from soybeans fermented by natto starter strains of Bacillus subtilis natto. It has been suggested that extracellular protease activity released by the bacteria are involved in the production of poly-gamma-glutamate (gamma-PGA) during natto fermentation. One of the natto starters, strain r22, possesses at least seven genes, each of which encoded an extracellular protease orthologous to its counterpart in B. subtilis 168, aprE, bpr, epr, mpr, nprE, vpr, and wprA, but it was found to lack nprB. Inactivating the aprE ortholog alone resulted in a severe decrease in gamma-PGA production and in the total extracellular protease activity. The defect in gamma-PGA production of the mutant lacking the aprE ortholog was complemented when the medium was supplemented with sufficient glutamate. These results suggest that the alkaline senile protease encoded by aprE plays an indispensable role in supplying materials to produce gamma-PGA. On the other hand, simultaneous inactivation of all the protease genes except for aprE did not significantly affect either gamma-PGA production or total protease activity.

    TAYLOR & FRANCIS LTD, 2013年04月, Bioscience, biotechnology, and biochemistry, 77 (4), 802 - 809, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Suzuki H, Okazaki F, Kondo A, Yoshida K

    2013年04月, Applied microbiology and biotechnology, 97 (7), 2929 - 2938

    [査読有り]

  • Shinji Takenaka, Ryosuke Nomura, Ayumi Minegishi, Ken-ichi Yoshida

    Background: The agrichemical 4-aminopyridine is used as a bird repellent in crop fields and has an epileptogenic action in a variety of animals, including man and mouse. 4-Aminopyridine is biodegraded in the environment through an unknown mechanism. Results: A 4-aminopyridine-degrading enrichment culture utilized 4-aminopyridine as a carbon, nitrogen, and energy source, generating 4-amino-3-hydroxypyridine, 3,4-dihydroxypyridine, and formate as intermediates. 4-Amino-3-hydroxypyridine could not be further metabolized and probably accumulated as a dead-end product in the culture. Biodegradability tests and partial sequence analysis of the enrichment culture indicated that 4-aminopyridine was mainly degraded via 3,4-dihydroxypyridine and that the metabolite is probably cleaved by 3-hydroxy-4-pyridone dioxygenase. Seven culturable predominant bacterial strains (strains 4AP-A to 4AP-G) were isolated on nutrient agar plates. Changes in the bacterial populations of 4-aminopyridine, 3,4-dihydroxypyridine, or formate/ammonium chloride enrichment cultures were monitored by denaturing gradient gel electrophoresis (DGGE) profiling of PCR-amplified 16S rRNA gene fragments. Sequence analysis of the 16S rRNA gene fragments derived from predominant DGGE bands indicated that Pseudomonas nitroreducens 4AP-A and Enterobacter sp. 4AP-G were predominant in the three tested enrichment cultures and that the unculturable strains Hyphomicrobium sp. 4AP-Y and Elizabethkingia sp. 4AP-Z were predominant in 4-aminopyridine and formate/ammonium chloride enrichment cultures and in the 3,4-dihydroxypyridine enrichment culture, respectively. Among the culturable strains, strain 4AP-A could utilize 3,4-dihydroxypyridine as a growth substrate. Although we could not isolate strain 4AP-Y on several media, PCR-DGGE analysis and microscopy indicated that the unique bi-polar filamentous bacterial cells gradually became more dominant with increasing 4-aminopyridine concentration in the medium. Conclusions: Hyphomicrobium sp. 4AP-Y, P. nitroreducens 4AP-A, and Elizabethkingia sp. 4AP-Z probably play important roles in 4-aminopyridine degradation in crop fields. In the enrichment culture, 3,4-dihydroxypyridine and its metabolites including formate might be shared as growth substrates and maintain the enrichment culture, including these indispensable strains.

    BIOMED CENTRAL LTD, 2013年03月, BMC MICROBIOLOGY, 13 (1), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ability of a perfluoropolymer membrane to tolerate by-products of ethanol fermentation broth from dilute acid-pretreated rice straw

    SASAKI Kengo, MATSUDA Fumio, HASUNUMA Tomohisa, OGINO Chiaki, URAIRI M, YOSHIDA K, KONDO Akihiko

    2013年01月, Biocehmical Engineering Journal, 70, 135 - 139, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Aryl hydrocarbon receptor enhances the expression of multidrug-resistant mdr1b through p53 in mouse hepatoma cells

    MITANI Takakazu, KINEHARA Masaki, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    2013年, Organohalogen Compounds, 75, 625 - 628, 英語

    研究論文(学術雑誌)

  • Hirokazu Suzuki, Ayano Murakami, Ken-ichi Yoshida

    Counterselection systems facilitate marker-free genetic modifications in microbes by enabling positive selections for both the introduction of a marker gene into the microbe and elimination of the marker from the microbe. Here we report a counterselection system for Geobacillus kaustophilus HTA426, established through simultaneous disruption of the pyrF and pyrR genes. The pyrF gene, essential for pyrimidine biosynthesis and metabolization of 5-fluoroorotic acid (5-FOA) to toxic metabolites, was disrupted by homologous recombination. The resultant MK54 strain (Delta pyrF) was auxotrophic for uracil and resistant to 5-FOA. MK54 complemented with pyrF was prototrophic for uracil but insensitive to 5-FOA in the presence of uracil. To confer 5-FOA sensitivity, the pyrR gene encoding an attenuator to repress pyrimidine biosynthesis by sensing uracil derivatives was disrupted. The resultant MK72 strain (Delta pyrF Delta pyrR) was auxotrophic for uracil and resistant to 5-FOA. MK72 complemented with pyrF was prototrophic for uracil and 5-FOA sensitive even in the presence of uracil. The results suggested that pyrF could serve as a counterselection marker in MK72, which was demonstrated by efficient marker-free integrations of heterologous beta-galactosidase and a-amylase genes. The integrated genes were functionally expressed in G. kaustophilus and conferred new functions on the thermophile. This report describes the first establishment of a pyrF-based counterselection system in a Bacillus-related bacterium, along with the first demonstration of homologous recombination and heterologous gene expression in G. kaustophilus. Our results also suggest a new strategy for establishment of counterselection systems.

    AMER SOC MICROBIOLOGY, 2012年10月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78 (20), 7376 - 7383, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hirokazu Suzuki, Ken-ichi Yoshida

    We established an efficient transformation method for thermophile Geobacillus kaustophilus HTA426 using conjugative transfer from Escherichia coli of host-mimicking plasmids that imitate DNA methylation of strain HTA426 to circumvent its DNA restriction barriers. Two conjugative plasmids, pSTE33T and pUCG18T, capable of shuttling between E. coli and Geobacillus spp., were constructed. The plasmids were first introduced into E. coli BR408, which expressed one inherent DNA methylase gene (dam) and two heterologous methylase genes from strain HTA426 (GK1380-GK1381 and GK0343-GK0344). The plasmids were then directly transferred from E. coli cells to strain HTA426 by conjugative transfer using pUB307 or pRK2013 as a helper plasmid. pUCG18T was introduced very efficiently (transfer efficiency, 10(-5)-10(-3) recipient(-1)). pSTE33T showed lower efficiency (10(-7)-10(-6) recipient(-1)) but had a high copy number and high segregational stability. Methylase genes in the donor substantially affected the transfer efficiency, demonstrating that the host-mimicking strategy contributes to efficient transformation. The transformation method, along with the two distinguishing plasmids, increases the potential of G kaustophilus HTA426 as a thermophilic host to be used in various applications and as a model for biological studies of this genus. Our results also demonstrate that conjugative transfer is a promising approach for introducing exogenous DNA into thermophiles.

    KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY, 2012年09月, JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 22 (9), 1279 - 1287, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ken-ichi Yoshida, Azusa Sanbongi, Ayano Murakami, Hirokazu Suzuki, Shinji Takenaka, Hideto Takami

    Geobacillus kaustophilus HTA426, a thermophilic Bacillus-related species, utilizes some inositol stereoisomers, including myo-, D-chiro- and scyllo-inositols (MI, DCI and SI), as sole carbon sources. Within its genome are three paralogous genes that possibly encode inositol dehydrogenase. These genes are located in tandem within a large gene cluster containing an almost complete set of iol genes homologous to genes involved in inositol catabolism in Bacillus subtilis. Each of the three plausible inositol dehydrogenases was purified as a His(6)-tag fusion. The enzymes exhibited thermophilic activity, each with its own characteristic specificity for the inositol stereoisomers and cofactors. Northern blot and primer extension analyses revealed that the three enzymes were encoded by the same 5 kb polycistronic transcript and were induced simultaneously in the presence of MI. HTA426 was subjected to ethyl methanesulfonate (EMS) mutagenesis to isolate a mutant strain, PS8, which was not able to utilize MI. In PS8, inositol dehydrogenase activity was abolished along with the 5 kb transcript, suggesting that any of the three enzymes supports MI-dependent growth. Analysis of metabolites in HTA426 cells grown in the presence of MI revealed that substantial amounts of DCI and SI appeared intracellularly during the stationary phase, while only MI was present in PS8 cells, suggesting that interconversion of inositol stereoisomers may involve these three enzymes.

    SOC GENERAL MICROBIOLOGY, 2012年08月, MICROBIOLOGY-SGM, 158 (8), 1942 - 1952, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Shinpei Hano, Minyi Cheng, Ken-ichi Yoshida, Kenji Aoki

    Eggshell membrane is a mechanically stable and insoluble cross-linked fibrous protein. strain ME-4 synthesizes a metalloprotease that degrades the eggshell membrane. We cloned the encoding gene in . The recombinant protease, over-expressed in , was inactive but addition of acetone to crude cell extracts restored the activity and removed many proteins. We purified the active, acetone-treated protease to homogeneity in a single chromatography step with 57% recovery. The recombinant protease partially hydrolyzed eggshell membrane and produced more soluble peptides and proteins than commercial elastase, alpha-chymotrypsin, and collagenase. The soluble peptides produced from hydrolyzed eggshell membrane inhibited angiotensin-I-converting enzyme activity. The degradation of eggshell membrane by the recombinant elastase could be applied to the production of soluble bioactive peptides.

    SPRINGER, 2012年05月, BIOTECHNOLOGY LETTERS, 34 (5), 949 - 955, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ranjita Biswas, Masaru Yamaoka, Hideki Nakayama, Takashi Kondo, Ken-ichi Yoshida, Virendra S. Bisaria, Akihiko Kondo

    Production of 2,3-butanediol by Bacillus subtilis takes place in late-log or stationary phase, depending on the expression of bdhA gene encoding acetoin reductase, which converts acetoin to 2,3-butanediol. The present work focuses on the development of a strain of B. subtilis for enhanced production of 2,3-butanediol in early log phase of growth cycle. For this, the bdhA gene was expressed under the control of P (alsSD) promoter of AlsSD operon for acetoin fermentation which served the substrate for 2,3-butanediol production. Addition of acetic acid in the medium induced the production of 2,3-butanediol by 2-fold. Two-step aerobic-anaerobic fermentation further enhanced 2,3-butanediol production by 4-fold in comparison to the control parental strain. Thus, addition of acetic acid and low dissolved oxygen in the medium are involved in activation of bdhA gene expression from P (alsSD) promoter in early log phase. Under the conditions tested in this work, the maximum production of 2,3-butanediol, 2.1 g/l from 10 g/l glucose, was obtained at 24 h. Furthermore, under the optimized microaerophilic condition, the production of 2,3-butanediol improved up to 6.1 g/l and overall productivity increased by 6.7-fold to 0.4 g/l h in the engineered strain compared to that in the parental control.

    SPRINGER, 2012年05月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94 (3), 651 - 658, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shin Nishiumi, Keizo Hosokawa, Masaki Anetai, Toshiro Shibata, Rie Mukai, Ken-ichi Yoshida, Hitoshi Ashida

    Transformation of an aryl hydrocarbon receptor (AhR) is the initial step to express the multiple toxicity of halogenated and polycyclic aromatic hydrocarbons (HAHs and PAHs) including dioxins. Therefore, it has been suggested that suppression of the transformation induced by HAHs and PAHs leads to reduce their toxicological effects. In this study, the antagonistic effect of 110 indigenous plants (192 plant parts) used as medicine and/or food by the Ainu on the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR transformation was investigated. Of these, a stalk of Aralia elata (Miq.) Seemann and a bark of Fraxinus mandshurica Rupr. var. japonica Maxim. exhibited the strong antagonistic effect in a dose-dependent manner. An antioxidative activity and polyphenol content were also measured, and the strong correlation (r= 0.96) between these two parameters could be confirmed. However, correlation coefficients of the antagonistic effect of 192 extracts compared to their antioxidative activity and polyphenol content were 0.17 and 0.20, respectively. These results suggest that the Ainu-selected traditional beneficial plants are useful source for findings of novel AhR antagonists, and the antagonistic activity of these plants may be independent on their antioxidative activity and polyphenol content.

    WILEY-BLACKWELL, 2012年04月, JOURNAL OF FOOD SCIENCE, 77 (4), C420 - C429, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 2Ap03 Bacillus subtilis cell factory for production of scyllo-inositol promising for Alzheimer's disease (Bio-Based Production) :

    YOSHIDA Ken-ichi, ONUMA Chumsakul, ISHIKAWA Shu, OGASAWARA Naotake

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 英語

    研究論文(研究会,シンポジウム資料等)

  • Masaru Yamaoka, Shin Osawa, Tetsuro Morinaga, Shinji Takenaka, Ken-ichi Yoshida

    Background: A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, is abundantly available. Results: Bacillus subtilis possesses a unique inositol metabolism involving both stereoisomers. We manipulated the inositol metabolism in B. subtilis to permit the possible bioconversion from myo-inositol to scyllo-inositol. Within 48 h of cultivation, the engineered strain was able to convert almost half of 10 g/L myo-inositol to scyllo-inositol that accumulated in the culture medium. Conclusions: The engineered B. subtilis serves as a prototype of cell factory enabling a novel and inexpensive supply of scyllo-inositol.

    BIOMED CENTRAL LTD, 2011年09月, MICROBIAL CELL FACTORIES, 10, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hirokazu Suzuki, Shunji Takahashi, Hiroyuki Osada, Ken-ichi Yoshida

    DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-GC(5m)CGGC-3' and 5'-GAG(5m)CTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.

    KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY, 2011年07月, JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 21 (7), 675 - 678, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shinji Takenaka, Nobutaka Yoshida, Ken-ichi Yoshida, Shuichiro Murakami, Kenji Aoki

    We cloned the genes encoding the two distinct extracellular halotolerant proteases of Bacillus subtilis FP-133 Expro-I and Expro-II, which were classified as alkaline serine and neutral proteases respectively. Three-dimensional modeling suggested that acidic and polar amino acid residues located on the surface stabilize protein structure in the presence of relatively high NaCl concentrations.

    TAYLOR & FRANCIS LTD, 2011年01月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75 (1), 148 - 151, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shin Nishiumi, Hiroaki Bessyo, Mayuko Kubo, Yukiko Aoki, Akihito Tanaka, Ken-ichi Yoshida, Hitoshi Ashida

    To investigate the preventive effects of tea on hyperglycemia and insulin resistance, male C57BL/6J mice were given a high-fat diet containing 29% lard and also green or black tea ad libitum for 14 weeks. Both teas suppressed body weight gain and deposition of white adipose tissue caused by the diet. In addition, they improved hyperglycemia and glucose intolerance by stimulating glucose uptake activity accompanied by the translocation of glucose transporter (GLUT) 4 to the plasma membrane in muscle. Long-term consumption of the high-fat diet reduced levels of insulin receptor beta-subunit, GLUT4 and AMP-activated protein kinase a in muscle, and green and black tea suppressed these decreases. The results strongly suggest that green and black tea suppress high-fat diet-evoked hyperglycemia and insulin resistance by retaining the level of GLUT4 and increasing the level of GLUT4 on the plasma membrane in muscle.

    AMER CHEMICAL SOC, 2010年12月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58 (24), 12916 - 12923, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Manabu Ueda, Takashi Furuyashiki, Kayo Yamada, Yukiko Aoki, Iwao Sakane, Itsuko Fukuda, Ken-ichi Yoshida, Hitoshi Ashida

    In this study, we investigated the effects of tea catechins on the translocation of glucose transporter (GLUT) 4 in 3T3-L1 adipocytes. We found that the ethyl acetate fraction of green tea extract, containing abundant catechins, most decreased insulin-induced glucose uptake activity in 3T3-L1 cells. When the cells were treated with 50 mu M catechins in the absence or presence of insulin for 30 min, nongallate-type catechins increased glucose uptake activity without insulin, whereas gallate-type catechins decreased insulin-induced glucose uptake activity. (-)-Epicatechin (EC) and (-)-epigallocatechin (EGC), nongallate-type catechins, increased glucose uptake activity in the dose- and time-dependent manner, whereas (-)-catechin 3-gallate (Cg) and (-)-epigallocatechin 3-gallate (EGCg), gallate-type catechins, decreased insulin-induced glucose uptake activity in the dose- and time-dependent manner. When the cells were treated with 50 mu M catechins for 30 min, EC and EGC promoted GLUT4 translocation, whereas Cg and EGCg decreased the insulin-induced translocation in the cells. EC and EGC increased phosphorylation of PKC lambda/zeta without phosphorylation of insulin receptor (IR) and Akt. Wortmannin and LY294002, inhibitors for phosphatidylinositol 3'-kinase (PI3K), decreased EC- and EGC-induced glucose uptake activity in the cells. Cg and EGCg decreased phosphorylation of PKC lambda/zeta in the presence of insulin without affecting insulin-induced phosphorylation of IR, and Akt. Therefore, EC and EGC promote the translocation of GLUT4 through activation of PI3K, and Cg and EGCg inhibit insulin-induced translocation of GLUT4 by the insulin signaling pathway in 3T3-L1 cells.

    ROYAL SOC CHEMISTRY, 2010年11月, FOOD & FUNCTION, 1 (2), 167 - 173, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rie Mukai, Yasuhito Shirai, Naoaki Saito, Itsuko Fukuda, Shin Nishiumi, Ken-ichi Yoshida, Hitoshi Ashida

    The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates biological and toxicological effects by binding to its agonists such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Previously we demonstrated that flavonoids suppressed the TCDD-induced DNA-binding activity of the AhR in a structure-dependent manner. In this study, we investigated the mechanisms by which flavonoids suppressed the AhR-mediated signal transduction in mouse hepatoma Hepa-1c1c7 cells. Flavones and flavonols suppressed the TCDD-induced nuclear translocation of the AhR and dissociation of its partner proteins, heat shock protein 90 and X-associated protein 2, whereas flavanones and catechins did not. Flavonoids of all these four subclasses suppressed the phosphorylation of both AhR and Arnt and the formation of a heterodimer consisting of these proteins. Since certain flavonoids are known to inhibit mitogen-activated protein kinases (MAPKs), we confirmed the contribution of MAPK/ERK kinase (MEK) to the AhR-mediated signal transduction by using U0126, an inhibitor of MEK1/2. U0126 suppressed TCDD-induced phosphorylation of the AhR and Arnt followed by the DNA-binding activity of the AhR. Flavanones and catechins suppressed the TCDD-induced phosphorylation of ERK1/2. The inhibition of MEK/ERK phosphorylation is one of the mechanisms by which flavanones and catechins suppress the AhR-mediated signal transduction in Hepa-1c1c7 cells. (C) 2010 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2010年09月, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 501 (1), 134 - 141, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takayuki Asahara, Yukiko Mori, Natalia P. Zakataeva, Vitaliy A. Livshits, Ken-ichi Yoshida, Kiyoshi Matsuno

    In order to test a possible approach to enhance fermentative inosine production by Bacillus subtilis, seven gene-targeted mutations were introduced in the laboratory standard strain168 in a stepwise fashion. The mutations were employed in order to prevent inosine 5'-monophosphate (IMP) from being consumed for AMP and GMP synthesis, to minimize inosine degradation, and to expand the intracellular IMP pool. First, the genes for adenylosuccinate synthase (purA) and IMP dehydrogenase (guaB) were inactivated. Second, two genes for purine nucleoside phosphorylase, punA and deoD, were inactivated. Third, to enhance purine nucleotide biosynthesis, the pur operon repressor PurR and the 5'-UTR of the operon, containing the guanine riboswitch, were disrupted. Finally, the -10 sequence of the pur promoter was optimized to elevate its transcription level. The resulting mutant was capable of producing 6 g/L inosine from 30 g/L glucose in culture broth without the detectable by-production of hypoxanthine. This indicates the validity of this approach for the breeding of the next generation of B. subtilis strains for industrial nucleoside production.

    SPRINGER, 2010年08月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87 (6), 2195 - 2207, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tetsuro Morinaga, Takatsugu Matsuse, Hitoshi Ashida, Ken-ichi Yoshida

    Bacillus subtilis IolT is the major myo-inositol transporter for growth, while IolF is a minor one unable to support growth. We found that either loIT or IolF was sufficient for moderate growth using D-chiro-inositol. Conversely to IolT, IolF transported n-chiro-inositol more preferentially than myo-inositol. These results indicate that IolT and IolF are different in substrate specificity.

    TAYLOR & FRANCIS LTD, 2010年06月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74 (6), 1312 - 1314, 英語

    [査読有り]

    研究論文(学術雑誌)

  • NISHIUMI Shin, YOSHIDA Masaru, AZUMA Takeshi, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes a wasting syndrome characterized by a loss of body weight accompanied by a decrease in adipose tissue weight, i.e., insulin resistance-like symptoms. Therefore, the effects of TCDD on an insulin signaling pathway in mature 3T3-L1 adipocytes were investigated to obtain insight into the underlying mechanisms. TCDD downregulated expression of insulin receptor beta-subunit (IR beta), insulin receptor substrate 1 (IRS1), and glucose transporter 4 (GLUT4) and decreased insulin-stimulated glucose uptake activity. TCDD also upregulated expression of TNF-alpha, one of insulin resistance-inducing factors. Anti-TNF-alpha neutralization antibody and silencing of TNF-alpha receptor 1 (TNFR1) diminished the TCDD-induced downregulation of IR beta, IRS1, and GLUT4. Moreover, the experiments using small interfering RNA for an aryl hydrocarbon receptor (AhR) revealed that the TCDD-evoked changes of IR beta, IRS1, GLUT4, and TNF-alpha were dependent on AhR. TCDD also stimulated the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK), and their inhibitors abrogated the TCDD-induced downregulation of IR beta, IRS1, and GLUT4; upregulation of TNF-alpha; and activation of NF-kappa B. Taken together, TCDD stimulates expression and secretion of TNF-alpha in adipocytes through activation of AhR, ERK1/2, and JNK, and the secreted TNF-alpha causes the downregulation of IR beta, IRS1, and GLUT4 through TNFR1, resulting in insulin resistance.

    OXFORD UNIV PRESS, 2010年06月, Toxicology Science, 115 (2), 482 - 491, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tetsuro Morinaga, Kazuo Kobayashi, Hitoshi Ashida, Yasutaro Fujita, Ken-ichi Yoshida

    The Bacillus subtilis asnH operon, comprising yxbB, yxbA, yxnB, asnH and yxaM, is induced dramatically in the transition between exponential growth and stationary phase in rich sporulation medium. The asnH operon is transcribed to produce an unstable long transcript covering the entire operon as well as a short one corresponding to the first three genes. Northern blot analysis revealed that the discrete band corresponding to the short transcript was detectable even 1 h after the addition of excess rifampicin, suggesting its unusual stability. The transcription start site of the operon was determined; its corresponding promoter was most likely sigma-A dependent and under tight control of AbrB and CodY. Within the 5'-proximal region of the transcript preceding yxbB, there is a mysterious long sequence triplication (LST) segment, consisting of a tandem repeat of two highly conserved 118 bp units and a less conserved 129 bp unit. This LST segment was not involved in regulation by AbrB and CodY. Transcriptional fusion of the 5'-region containing the LST segment to lacZ resulted in a significant increase in beta-galactosidase synthesis in cells; the LST segment was thought to prevent degradation of the 5'-region-lacZ fusion transcript. These results suggest that the 5'-region containing the LST segment could function as an mRNA stabilizer that prolongs the lifetime of the transcript to which it is fused.

    SOC GENERAL MICROBIOLOGY, 2010年06月, MICROBIOLOGY-SGM, 156 (6), 1632 - 1641, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shin Nishiumi, Masaru Yoshida, Takeshi Azuma, Ken-ichi Yoshida, Hitoshi Ashida

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes a wasting syndrome characterized by a loss of body weight accompanied by a decrease in adipose tissue weight, i.e., insulin resistance-like symptoms. Therefore, the effects of TCDD on an insulin signaling pathway in mature 3T3-L1 adipocytes were investigated to obtain insight into the underlying mechanisms. TCDD downregulated expression of insulin receptor beta-subunit (IR beta), insulin receptor substrate 1 (IRS1), and glucose transporter 4 (GLUT4) and decreased insulin-stimulated glucose uptake activity. TCDD also upregulated expression of TNF-alpha, one of insulin resistance-inducing factors. Anti-TNF-alpha neutralization antibody and silencing of TNF-alpha receptor 1 (TNFR1) diminished the TCDD-induced downregulation of IR beta, IRS1, and GLUT4. Moreover, the experiments using small interfering RNA for an aryl hydrocarbon receptor (AhR) revealed that the TCDD-evoked changes of IR beta, IRS1, GLUT4, and TNF-alpha were dependent on AhR. TCDD also stimulated the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK), and their inhibitors abrogated the TCDD-induced downregulation of IR beta, IRS1, and GLUT4; upregulation of TNF-alpha; and activation of NF-kappa B. Taken together, TCDD stimulates expression and secretion of TNF-alpha in adipocytes through activation of AhR, ERK1/2, and JNK, and the secreted TNF-alpha causes the downregulation of IR beta, IRS1, and GLUT4 through TNFR1, resulting in insulin resistance.

    OXFORD UNIV PRESS, 2010年06月, TOXICOLOGICAL SCIENCES, 115 (2), 482 - 491, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nhung Thuy Dang, Rie Mukai, Ken-ichi Yoshida, Hitoshi Ashida

    Diabetes mellitus is a complex disease that is characterized by the defection of insulin sensitivity in such peripheral tissues as skeletal muscle, adipose tissue and liver. We have previously demonstrated that certain inositol derivatives stimulated glucose uptake accompanied by the translocation of glucose transporter 4 (GLUT4) to the plasma membrane in L6 myotubes. We investigated in this present study whether an oral intake of D-pinitol (PI) and myo-inositol (MI) would affect GLUT4 translocation in the skeletal muscle of mice. PI or MI at 1 g/kg BW administered orally to mice 30 min before a post-oral injection of glucose at 2 g/kg BW resulted in both PI and MI increasing GLUT4 translocation in the skeletal muscle and lowering the plasma glucose and insulin levels. PI and MI, therefore, have the potential to prevent diabetes mellitus by reducing the postprandial blood glucose level and stimulating GLUT4 translocation in the skeletal muscle.

    TAYLOR & FRANCIS LTD, 2010年05月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74 (5), 1062 - 1067, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tetsuro Morinaga, Hitoshi Ashida, Ken-ichi Yoshida

    scyllo-Inositol (SI) is a stereoisomer of inositol whose catabolism has not been characterized in bacteria. We found that Bacillus subtilis 168 was able to grow using SI as its sole carbon source and that this growth was dependent on a functional iol operon for catabolism of myo-inositol (MI; another inositol isomer, which is abundant in nature). Previous studies elucidated the MI catabolic pathway in B. subtilis as comprising multiple stepwise reactions catalysed by a series of lol enzymes. The first step of the pathway converts MI to scyllo-inosose (SIS) and involves the MI dehydrogenase lolG. Since lolG does not act on SI, we suspected that there could be another enzyme converting SI into SIS, namely an SI dehydrogenase. Within the whole genome, seven genes paralogous to iolG have been identified and two of these, iolX and iolW (formerly known as yisS and yvaA, respectively), were selected as candidate genes for the putative SI dehydrogenase since they were both prominently expressed when B. subtilis was grown on medium containing SI. iolX and iolW were cloned in Eschefichia colt and both were shown to encode a functional enzyme, revealing the two distinct SI dehydrogenases in B. subtilis. Since inactivation of iolX impaired growth with SI as the carbon source, lolX was identified as a catabolic enzyme required for SI catabolism and it was shown to be NAD(+) dependent. The physiological role of lolW remains unclear, but it may be capable of producing SI from SIS with NADPH oxidation.

    SOC GENERAL MICROBIOLOGY, 2010年05月, MICROBIOLOGY-SGM, 156 (5), 1538 - 1546, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Manabu Ueda, Takashi Furuyashiki, Kayo Yamada, Yukiko Aoki, Itsuko Fukuda, Ken-ichi Yoshida, Hitoshi Ashida

    2010年, Food and Function, 1(2), 167-173, 英語

    [査読有り]

    研究論文(学術雑誌)

  • KINEHARA Masaki, FUKUDA Itsuko, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    Upon binding to ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an aryl hydrocarbon receptor (AhR) is activated to form a heterodimer with an aryl hydrocarbon receptor nuclear translocator (Arnt). This complex binds to DNA. It has been shown that the AhR bonds to a DNA sequence called the dioxin response element (DRE), which controls the expression of battery genes. It is reported that TCDD releases arachidonic acid from membrane phospholipids via activation of phospholipase A(2)s (PLA(2)s) in various cell types. Recently, we demonstrated that the TCDD-activated AhR binds to the second intron of the Pla2g4a gene, which encodes cytosolic phospholipase A(2)alpha (cPLA(2)alpha), in mouse hepatoma Hepa-1c1c7 cells. This result suggests that Pla2g4a appears to be a target gene of the AhR. In the present study, we investigated whether the transcriptional regulation of Pla2g4a is dependent on the AhR in Hepa-1c1c7 cells. Treatment of the cells with TCDD increased mRNA expression of Pla2g4a and enzymatic activity of PLA(2). while this increased expression was not observed in AhR-defective c12 cells. After transient transfection of an Ahr gene-expressing plasmid into the 02 cells, expression of Pla2g4a was increased by TCDD. These results indicate that Pla2g4a may be a novel target gene of the AhR, and its transcriptional induction is mediated through binding of the AhR to the second intron of Pla2g4a, although this target site does not have a typical DRE sequence. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年10月, Journal of Bioscience and Bioengineering, 108 (4), 277 - 281, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masaki Kinehara, Itsuko Fukuda, Ken-ichi Yoshida, Hitoshi Ashida

    Upon binding to ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an aryl hydrocarbon receptor (AhR) is activated to form a heterodimer with an aryl hydrocarbon receptor nuclear translocator (Arnt). This complex binds to DNA. It has been shown that the AhR bonds to a DNA sequence called the dioxin response element (DRE), which controls the expression of battery genes. It is reported that TCDD releases arachidonic acid from membrane phospholipids via activation of phospholipase A(2)s (PLA(2)s) in various cell types. Recently, we demonstrated that the TCDD-activated AhR binds to the second intron of the Pla2g4a gene, which encodes cytosolic phospholipase A(2)alpha (cPLA(2)alpha), in mouse hepatoma Hepa-1c1c7 cells. This result suggests that Pla2g4a appears to be a target gene of the AhR. In the present study, we investigated whether the transcriptional regulation of Pla2g4a is dependent on the AhR in Hepa-1c1c7 cells. Treatment of the cells with TCDD increased mRNA expression of Pla2g4a and enzymatic activity of PLA(2). while this increased expression was not observed in AhR-defective c12 cells. After transient transfection of an Ahr gene-expressing plasmid into the 02 cells, expression of Pla2g4a was increased by TCDD. These results indicate that Pla2g4a may be a novel target gene of the AhR, and its transcriptional induction is mediated through binding of the AhR to the second intron of Pla2g4a, although this target site does not have a typical DRE sequence. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年10月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108 (4), 277 - 281, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rie Mukai, Yasuhito Shirai, Naoaki Saito, Ken-ichi Yoshida, Hitoshi Ashida

    Flavonoids are widely distributed in the plant kingdom and show various biological activities. The bioavailability of flavonoids in biological samples has conventionally been quantified by high-performance liquid chromatography and mass spectrometry, but with these analytical techniques it is difficult to estimate the subcellular localization of flavonoids in intact cells. In this study, we attempted to examine the localization of flavonoids in cultured cells using a confocal laser scanning fluorescence microscope and mouse hepatoma Hepa-1c1c7 cells. Five flavonol aglycones showed autofluorescence in the cells under the conditions (Ex. 488 nm to Em. 515-535 nm), whereas three flavonol glycosides and eight compounds belonging to other flavonoid subclasses, i.e., flavones, flavanones, and catechins, did not. The autofluorescence of galangin and kaempferol appeared stronger in the nucleus than cytoplasm, suggesting that they are incorporated into the cells and accumulated in the nucleus. The proposed method provided evidence that flavonol aglycones are incorporated into, and accumulated in the nucleus of, hepatocytes.

    SPRINGER, 2009年04月, CYTOTECHNOLOGY, 59 (3), 177 - 182, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideyuki Goto, Yuji Kumada, Hitoshi Ashida, Ken-ichi Yoshida

    A number of 2',3',4'-trihydroxy-2-phenylacetophenone derivatives were synthesized and examined for growth inhibition of several kinds of bacteria. 2',3',4'-Trihydroxy-2-phenylacetophenone itself exhibited no antibacterial activity, but some of its derivatives showed various antibacterial activities depending on functional groups introduced on the 2-phenyl ring. Eighteen out of 24 compounds synthesized in this study appeared to possess antibacterial activities against at least two Gram-positive strains of Bacillus subtilis and Staphylococcus aureus, 2-(biphenyl-4-yl)-2',3',4'-trihydroxy-acetophenone being the most active with LC50 of 5.8 mu M and 5.6 mu M respectively. However, none of the synthesized compounds exhibited inhibitory effects on Gram-negative strains, such as Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Salmonella enterica, suggesting that anti-Gram-positive specificity of the antibacterial compounds.

    TAYLOR & FRANCIS LTD, 2009年01月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73 (1), 124 - 128, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ueda M, Nishiumi S, Nagayasu H, Fukuda I, Yoshida K, Ashida H

    2008年12月, Biochemical and biophysical research communications, 377 (1), 286 - 290

    [査読有り]

  • Mukai R, Fukuda I, Nishiumi S, Natsume M, Osakabe N, Yoshida K, Ashida H

    2008年11月, Journal of agricultural and food chemistry, 56 (21), 10399 - 10405

    [査読有り]

  • Kada S, Yabusaki M, Kaga T, Ashida H, Yoshida K

    2008年07月, Bioscience, biotechnology, and biochemistry, 72 (7), 1869 - 1876

    [査読有り]

  • Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y

    2008年04月, The Journal of biological chemistry, 283 (16), 10415 - 10424

    [査読有り]

  • Nishiumi S, Yamamoto N, Kodoi R, Fukuda I, Yoshida K, Ashida H

    2008年02月, Archives of biochemistry and biophysics, 470 (2), 187 - 199

    [査読有り]

  • Suppressive effects of propolis extract on cytochrome P4501A1 expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin

    Daisuke Kashiwada, Itsuko Fukuda, Ken-ichi Yoshida, Hitoshi Ashida

    2008年, Journal of Clinical Biochemistry and Nutrition, 43(Suppl.1), 460 - 463, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Angeline Yap, Shin Nishiumi, Ken-Ichi Yoshida, Hitoshi Ashida

    Some of inositol derivatives have been reported to help the action of insulin stimulating glucose uptake in skeletal muscle cells. Rat L6 myotubes were employed in an attempt to develop an in vitro model system for investigation of the possible insulin-like effect of eight inositol derivatives, namely allo-inositol, D-chiro-inositol L-chiro-inositol, epi-inositol, muco-inositol, myo-inositol, scyllo-inositol and D-pinitol. At a higher concentration of 1 mM seven inositol derivatives other than myo-inositol were able to stimulate glucose uptake, while at 0.1 mM seven inositol derivatives other than myo-inositol were able to stimulate glucose uptake, while at 0.1mM only D-chiro-inositol, L-chiro-inositol, epi-inositol and muco-inositol could induce glucose uptake, indicating their significant insulin-mimetic activity. Immunoblot analyses revealed that at least D-chiro-inositol, L-chiro-inositol, epi-inositol, muco-inositol and D-pinitol were able to induce translocation of glucose transporter 4 (GLUT4) to plasma membrane not only in L6 myotubes but also in skeletal muscles of rats ex vivo. These results demonstrated that L6 myotubes appeared efficient as an in vitro system to identify inositol derivatives exerting an insulin-like effect on muscle cells depending on the induced translocation of GLUT4.

    SPRINGER, 2007年12月, CYTOTECHNOLOGY, 55 (2-3), 103 - 108, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shin Nishiumi, Ken-ichi Yoshida, Hitoshi Ashida

    Halogenated and polycyclic aromatic hydrocarbons induce diverse biochemical responses through the transformation of a cytosolic aryl hydrocarbon receptor (AhR). In mouse hepatoma Hepa-1c1c7 cells, curcumin, a yellow pigment of Curcuma longa, did not inhibit the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced translocation of the AhR into the nucleus, but rather accelerated it. In the nucleus, curcumin inhibited the TCDD-induced heterodimerization of the AhR with an AhR nuclear translocator (Arnt), an essential partner for the transformation, and also dose-dependently inhibited the TCDD-evoked phosphorylation of both the AhR and Arnt. Moreover, curcumin significantly inhibited the TCDD-induced activation of protein kinase C (PKC), which is involved in the transformation, decreased the TCDD-induced DNA-binding activity of the AhR/Arnt heterodimer, and downregulated CYP1A1 expression. In a cell-free system, curcumin inhibited the binding of 3-methylcholanthrene, an AhR agonist, to the receptor. These results indicate that curcumin is able to bind to the AhR as a ligand, but suppresses its transformation by inhibiting the phosphorylation of AhR and Arm, probably by PKC. (c) 2007 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2007年10月, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 466 (2), 267 - 273, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita

    Bacillus subtilis LmrA is known to be a repressor that regulates the hnrAB and yxaGH operons; lmrB and yxaG encode a multidrug resistance pump and quercetin 2,3-dioxygenase, respectively. DNase I footprinting analysis revealed that LmrA and YxaF, which are paralogous to each other, bind specifically to almost the same cis sequences, LmrA/YxaF boxes, located in the promoter regions of the hnrAB operon, the yxaF gene, and the yxaGH operon for their repression and containing a consensus sequence of AWTATAtagaNYGgTCTA, where W, Y, and N stand for A or T, C or T, and any base, respectively (three-out-of-four match [in lowercase type]). Gel retardation analysis indicated that out of the eight flavonoids tested, quercetin, fisetin, and catechin are most inhibitory for LmrA to DNA binding, whereas quercetin, fisetin, tamarixetin, and galangin are most inhibitory for YxaF. Also, YxaF bound most tightly to the tandem LmrA/YxaF boxes in the yxaGH promoter region. The lacZ fusion experiments essentially supported the above-mentioned in vitro results, except that galangin did not activate the lmrAB and yxaGH promoters, probably due to its poor incorporation into cells. Thus, the LmrA/YxaF regulon presumably comprising the 1mrAB operon, the yxaF gene, and the yxaGH operon is induced in response to certain flavonoids. The in vivo experiments to examine the regulation of the synthesis of the reporter P-galactosidase and quercetin 2,3-dioxgenase as well as that of multidrug resistance suggested that LmrA represses the hnrAB and yxaGH operons but that YxaF represses yxaGH more preferentially.

    AMER SOC MICROBIOLOGY, 2007年07月, JOURNAL OF BACTERIOLOGY, 189 (14), 5170 - 5182, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ken-ichi Yoshida, Won-Seok Kim, Masaki Kinehara, Rie Mukai, Hitoshi Ashida, Hideki Ikeda, Yasutaro Fujita, Hari B. Krishnan

    Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently WE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An WE ortholog was cloned from USDA191. USDA191 WE was expressed in Escherichia coli as a His(6)-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 WE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis WE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myoinositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and WE.

    TAYLOR & FRANCIS LTD, 2006年12月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (12), 2957 - 2964, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Predicting metals sensed by ArsR-SmtB repressors: allosteric interference by a non-effector metal

    吉田 健一

    2006年11月, Mol. Microbiol., 72, 1310-1315., 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takeshi Matsui, Machiko Hori, Nobuko Shizawa, Ideki Nakayama, Atsuhiko Shinmyo, Kazuya Yoshida

    Horseradish peroxidase isozyme C1a (HRP C1a) is widely used as a reporter enzyme in a variety of detection procedures such as enzyme-linked immunosorbent assay (ELISA) and western blotting. We previously isolated the gene encoding HRP C1a and showed that HRP C1a is at first translated as a preproprotein containing propeptides at its N- and C-termini (N-terminal secretion signal peptide and C-terminal propeptide; CTPP). The signal peptide (sp) is necessary for endoplasmic reticulum (ER) translocation and the CTPP acts as a vacuolar sorting determinant. Furthermore, HRP C1a was secreted into the culture medium from cells expressing the HRP C1a gene without the CTPP region. We optimized the secretory production system of HRP C1a in tobacco plants. To determine a suitable signal peptide for high-efficient secretion of proteins, three types of sp derived from HRP C1a (C1Psp), beta-D-glucan exohydrolase (GEsp) and 38 kDa peroxidase (38Psp) were compared. GE and 38P are secretory proteins highly accumulated in the culture medium of BY2 cells. The secretion efficiency was increased by 34% and 53% when GEsp and 38Psp was used, respectively. Next, we used a translational enhancer, the 5'-untranslated region of Nicotiana tabacum alcohol dehydrogenase gene (NtADH 5'-UTR). The production of HRP C1a was increased by placing NtADH 5'UTR in front of the ORF in BY2 cells. These results indicate that the localization and expression level of recombinant proteins can be controlled by the use of propeptides and 5'UTR, respectively. Finally, high-efficiency secretory production of the HRP C1a was also achieved in transgenic tobacco.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2006年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102 (2), 102 - 109, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tetsuro Morinaga, Masanori Yamaguchi, Yuki Makino, Hideaki Nanamiya, Kiwamu Takahashi, Hirofumi Yoshikawa, Fujio Kawamura, Hitoshi Ashida, Ken-ichi Yoshida

    Soybeans are rich in pinitol (PI; 3-O-methyl-D-chiroinositol), which improves health by treating conditions associated with insulin resistance, such as diabetes mellitus and obesity. Natto is a food made from soybeans fermented by strains of Bacillus subtilis natto. In the chromosome of natto strain OK2, there is a putative promoter region almost identical to the iol promoter for myo-inositol (MI) catabolic genes of B. subtilis 168. In the presence of MI, the putative iol promoter functioned to induce inositol dehydrogenase, the enzyme for the first-step reaction in the MI catabolic pathway. PI also induced inositol dehydrogenase and the promoter was indispensable for the utilization of PI as well as MI, suggesting that PI might be an alternative carbon source metabolized in a way involving the MI catabolic genes. Natto fermentation studies have revealed that the parental natto strain consumed PI while a mutant defective in the iol promoter did not do so at all. These results suggest that inactivating the MI catabolic genes might prevent PI consumption, retaining it in natto for enrichment of possible health-promoting properties.

    TAYLOR & FRANCIS LTD, 2006年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (8), 1913 - 1920, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Screening of indigenous plants from Japan for modulating effects on transformation of the aryl hydrocarbon receptor.

    Nishiumi S, Hosokawa K, Mukai R, Fukuda I, Hishida A, Iida O, Yoshida K, Ashida H

    2006年04月, Asian Pacific journal of cancer prevention : APJCP, 7 (2), 208 - 220

    [査読有り]

  • K Yoshida, M Yamaguchi, T Morinaga, M Ikeuchi, M Kinehara, H Ashida

    D-chiro-Inositol (DCI) is a drug candidate for the treatment of type 2 diabetes and polycystic ovary syndrome, since it improves the efficiency with which the body uses insulin and also promotes ovulation. Here, we report genetic modification of Bacillus subtilis for production of DCI from myo-inositol (MI). The B. subtilis iolABC-DEFGHIJ operon encodes enzymes for the multiple steps of the MI catabolic pathway. In the first and second steps, MI is converted to 2-keto-MI (2KMI) by IoIG and then to 3(D)-(3,5/4)-trihydroxycyclohexane-1,2-dione by IoIE. In this study, we identified ioll encoding inosose isomerase, which converts 2KMI to 1-keto-(D)-chiro-inositol (1KDCI), and found that IoIG reduces 1KDCI to DCI. Inactivation of iolE in a mutant constitutively expressing the iol operon blocked the MI catabolic pathway to accumulate 2KMI, which was converted to DCI via the activity of loll and IoIG. The mutant was able to convert at least 6% of input MI in the culture medium to DCI.

    AMER SOC MICROBIOLOGY, 2006年02月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72 (2), 1310 - 1315, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Screening of the indigenous plants from Japan for modulating effects on transformation of the aryl hydrocarbon receptor

    Shin Nishiumi, Keizo Hosokawa, Rie Mukai, Itsuko Fukuda, Atsuyuki Hishida, Osamu Iida, Ken-ichi Yoshida, Hitoshi Ashida

    2006年, Asian Pacific Journal of Cancer Prevention, 7(2), 208-220, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Suppressive effects of catechins on differentiation of 3T3-L1 preadipocytes

    AOKI Y, HASHIMOTO Takashi, YOSHIDA K, ASHIDA Hitoshi

    2005年03月, Proceedings of 2004 International Conference on O-CHA(tea) Culture and Science, 547-548, 英語

    研究論文(国際会議プロシーディングス)

  • Black tea (Camellia sinensis) suppresses hyperglycemia in STZ-induced diabetic rats

    KUBO M, SAKANE I, SAWAMURA S, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    2005年03月, Proceedings of 2004 International Conference on O-CHA (tea) Culture and Science, 561-562, 英語

    研究論文(国際会議プロシーディングス)

  • Tea has the potential to reduce the dioxin risk

    FUKUDA Itsuko, SAKANE I, NISHIUMI Shin, SHIRASUGI S, SAWAMURA S, KANAZAWA Kazuki, YOSHIDA Kenichi, ASHIDA Hitoshi

    2005年, Proceedings of 2004 International Conference on O-CHA(tea) Culture and Science, 594-595, 英語

    研究論文(国際会議プロシーディングス)

  • H Nakayama, K Yoshida, A Shinmyo

    In plants, the plasma membrane Na+/H+ antiporter is the only key enzyme that extrudes cytosolic Na+ and contributes to salt tolerance. But in fungi, the plasma membrane Na+/H+ antiporter and Na+-ATPase are known to be key enzymes for salt tolerance. Saccharomyces cerevisiae Ena1p ATPase encoded by the ENA1/PMR2A gene is primarily responsible for Na+ and Li+ efflux across the plasma membrane during salt stress and for K+ efflux at high pH and high K+. To test if the yeast ATPase would improve salt tolerance in plants, we expressed a triple hemagglutinin (HA)-tagged Ena1p (Ena1p-3HA) in cultured tobacco (Nicotiana tabacum L.) cv Bright Yellow 2 (BY2) cells. The Ena1p-3HA proteins were correctly localized to the plasma membrane of transgenic BY2 cells and conferred increased NaCl and LiCl tolerance to the cells. Under moderate salt stress conditions, the Ena1p-3HA-expressing BY2 clones accumulated lower levels of Na+ and Li+ than nonexpressing BY2 clones. Moreover, the Ena1p-3HA expressing BY2 clones accumulated lower levels of K+ than nonexpressing cells under no-stress conditions. These results suggest that the yeast Ena1p can also function as an alkali-cation (Na+, Li+, and K+) ATPase and alter alkali-cation homeostasis in plant cells. We conclude that, even with K+-ATPase activity, Na+-ATPase activity of the yeast Ena1p confers increased salt tolerance to plant cells during salt stress. (C) 2004 Wiley Periodicals, Inc.

    WILEY-BLACKWELL, 2004年03月, BIOTECHNOLOGY AND BIOENGINEERING, 85 (7), 776 - 789, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T Matsui, H Nakayama, K Yoshida, A Shinmyo

    Peroxidases (PRX, EC 1.11.1.7) are widely distributed across microorganisms, plants, and animals; and, in plants, they have been implicated in a variety of secondary metabolic reactions. In particular, horseradish (Armoracia rusticana) root represents the main source of commercial PRX production. The prxC1a gene, which encodes horseradish PRX (HRP) C, is expressed mainly in the roots and stems of the horseradish plant. HRP C1a protein is shown to be synthesized as a preprotein with both a N-terminal (NTPP) and a C-terminal propeptide (CTPP). These propeptides, which might be responsible for intracellular localization or secretion, are removed before or concomitant with production of the mature protein. We investigated the functional role of HRP C1a NTPP and CTPP in the determination of the vesicular transport route, using an analytical system of transgenically cultured tobacco cells (Nicotiana tabacum, BY2). Here, we report that NTPP and CTPP are necessary and sufficient for accurate localization of mature HRP C1a protein to vacuoles of the vesicular transport system. We also demonstrate that HRP C1a derived from a preprotein lacking CTPP is shunted into the secretory pathway.

    SPRINGER-VERLAG, 2003年10月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 62 (5-6), 517 - 522, 英語

    [査読有り]

    研究論文(学術雑誌)

  • [DNA microarray analysis of Bacillus subtilis].

    Yamaguchi H, Yoshida K, Fujita Y

    2003年05月, Seikagaku. The Journal of Japanese Biochemical Society, 75 (5), 407 - 410

    [査読有り]

  • K Kobayashi, SD Ehrlich, A Albertini, G Amati, KK Andersen, M Arnaud, K Asai, S Ashikaga, S Aymerich, P Bessieres, F Boland, SC Brignell, S Bron, K Bunai, J Chapuis, LC Christiansen, A Danchin, M Debarbouille, E Dervyn, E Deuerling, K Devine, SK Devine, O Dreesen, J Errington, S Fillinger, SJ Foster, Y Fujita, A Galizzi, R Gardan, C Eschevins, T Fukushima, K Haga, CR Harwood, M Hecker, D Hosoya, MF Hullo, H Kakeshita, D Karamata, Y Kasahara, F Kawamura, K Koga, P Koski, R Kuwana, D Imamura, M Ishimaru, S Ishikawa, Ishio, I, D Le Coq, A Masson, C Mauel, R Meima, RP Mellado, A Moir, S Moriya, E Nagakawa, H Nanamiya, S Nakai, P Nygaard, M Ogura, T Ohanan, M O'Reilly, M O'Rourke, Z Pragai, HM Pooley, G Rapoport, JP Rawlins, LA Rivas, C Rivolta, A Sadaie, Y Sadaie, M Sarvas, T Sato, HH Saxild, E Scanlan, W Schumann, JFML Seegers, J Sekiguchi, A Sekowska, SJ Seror, M Simon, P Stragier, R Studer, H Takamatsu, T Tanaka, M Takeuchi, HB Thomaides, Vagner, V, JM van Dijl, K Watabe, A Wipat, H Yamamoto, M Yamamoto, Y Yamamoto, K Yamane, K Yata, K Yoshida, H Yoshikawa, U Zuber, N Ogasawara

    To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximate to4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from bacteria with small genomes. Unexpectedly, most genes involved in the Embden-Meyerhof-Parnas pathway are essential. Identification of unknown and unexpected essential genes opens research avenues to better understanding of processes that sustain bacterial life.

    NATL ACAD SCIENCES, 2003年04月, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (8), 4678 - 4683, 英語

    研究論文(学術雑誌)

  • Ken-Ichi Yoshida, Yoshiyuki Yamamoto, Kaoru Omae, Mami Yamamoto, Yasutaro Fujita

    Among hundreds of mutants constructed systematically by the Japanese groups participating in the functional analysis of the Bacillus subtilis genome project, we found that a mutant with inactivation of iolT (ydjK) exhibited a growth defect on myo-inositol as the sole carbon source. The putative product of iolT exhibits significant similarity with many bacterial sugar transporters in the databases. In B. subtilis, the iolABCDEFGHIJ and iolRS operons are known to be involved in inositol utilization, and its transcription is regulated by the IolR repressor and induced by inositol. Among the iol genes, iolF was predicted to encode an inositol transporter. Inactivation of iolF alone did not cause such an obvious growth defect on inositol as the iolT inactivation, while simultaneous inactivation of the two genes led to a more severe defect than the single iolT inactivation. Determination of inositol uptake by the mutants revealed that iolT inactivation almost completely abolished uptake, but uptake by IolF itself was slightly detectable. These results, as well as the K(m) and V(max) values for the IolT and IolF inositol transporters, indicated that iolT and iolF encode major and minor inositol transporters, respectively. Northern and primer extension analyses of iolT transcription revealed that the gene is monocistronically transcribed from a promoter likely recognized by final sigma(A) RNA polymerase and negatively regulated by IolR as well. The interaction between IolR and the iolT promoter region was analyzed by means of gel retardation and DNase I footprinting experiments, it being suggested that the mode of interaction is quite similar to that found for the promoter regions of the iol divergon.

    2002年02月, Journal of bacteriology, 184 (4), 983 - 91, 英語, 国際誌

    [査読有り]

  • Yoshida, K, Kobayashi, K, Miwa, Y, Kang, C, Matsunaga, M, Yamaguchi, H, Tojo, S, Yamamoto, M, Nishi, R, Ogasawara, N, Nakayama, T, Fujita, Y

    2001年, Nucleic Acids Res., 29 (3), 683 - 692

    [査読有り]

  • Kobayashi, K, Ogura, M, Yamaguchi, H, Yoshida, K, Ogasawara, N, Tanaka, T, Fujita, Y

    2001年, J. Bacteriol., 183 (24), 7365 - 7370

    [査読有り]

  • Ogura, M, Yamaguchi, H, Yoshida, K, Fujita, Y, Tanaka, T

    2001年, Nucleic Acids Res., 29 (18), 3804 - 3813

    [査読有り]

  • L Winstedt, KI Yoshida, Y Fujita, C von Wachenfeldt

    Under aerobic conditions Bacllus subtilis utilizes a branched electron transport chain comprising various cytochromes and terminal oxidases. At present there is evidence for three types of terminal oxidases in B. subtilis: a caa(3)-, an aa(3)-, and a bd-type oxidase. We report here the cloning of the structural genes (cydA and cydB) encoding the cytochrome bd complex. Downstream of the structural genes, cydC and cydD are located. These genes encode proteins showing similarity to bacterial ATP-binding cassette (ABC)-type transporters. Analysis of isolated cell membranes showed that inactivation of cydA or deletion of cydABCD resulted in the loss of spectral features associated with cytochrome bd. Gene disruption experiments and complementation analysis showed that the cydC and cydD gene products are required for the expression of a functional cytochrome bd complex. Disruption of the cyd genes had no apparent effect on the growth of cells in broth or defined media. The expression of the cydABCD operon was investigated by Northern blot analysis and by transcriptional and translational cyd-lacZ fusions. Northern blot analysis confirmed that cydABCD is transcribed as a polycistronic message. The operon was found to be expressed maximally under conditions of low oxygen tension.

    AMER SOC MICROBIOLOGY, 1998年12月, JOURNAL OF BACTERIOLOGY, 180 (24), 6571 - 6580, 英語

    [査読有り]

    研究論文(学術雑誌)

  • KI Yoshida, D Aoyama, Ishio, I, T Shibayama, Y Fujita

    Previous determination of the nucleotide sequence of the ioi region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iolJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analysis suggested that an inositol-inducible promoter for the iol operon (iol promoter) might be a promoter-like sequence in the 5' region of iolA, which is probably recognized by sigma(A). SB nuclease analysis implied that a rho-independent terminator like structure in the 3' region of iolJ might be a terminator for iol transcription. Disruption of the iol promoter prevented synthesis of the iol transcript as well as that of Idh, implying that the iol operon is most probably transcribed as an 11.5-kb mRNA containing the 10 iol genes. Immediately upstream of the iol operon, two genes (iolR and iolS) with divergent orientations to the iol operon were found. Disruption of iolR (but not iolS) caused constitutive synthesis of the iol transcript and Idh, indicating that the iolR gene encodes a transcription-negative regulator (presumably a repressor) for the iol operon. Northern and S1 nuclease analyses revealed that the iolRS genes were cotranscribed from another inositol-inducible promoter, which is probably recognized by sigma(A). The promoter assignments of the iol and iolRS operons were confirmed in vivo with a lacZ fusion integrated into the amyE locus.

    AMER SOC MICROBIOLOGY, 1997年07月, JOURNAL OF BACTERIOLOGY, 179 (14), 4591 - 4598, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • FEMS2019報告

    吉田健一

    2019年11月, 生物工学会誌, 97 (11), 692 - 693, 日本語

    [査読有り][招待有り]

    会議報告等

  • 微生物のゲノム(前編)

    吉田 健一

    日本工業出版, 2015年, 環境浄化技術, 14 (7-8), 92 - 97, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 微生物のゲノム(後編)

    吉田 健一

    日本工業出版, 2015年, 環境浄化技術, 14 (9-10), 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • アルツハイマー病治療薬として期待されるシロイノシトールの微生物生産

    吉田 健一

    2015年, バイオサイエンスとインダストリー, 73 (4), 284 - 287, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 枯草菌を活用する生理活性イノシトールの開発

    吉田 健一

    日本生物工学会, 2013年11月, 生物工学会誌, 91 (11), 625 - 628, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 吉田 健一

    日本農芸化学会, 2012年10月, 化学と生物, 55 (10), 704 - 705, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 代謝アドオンシステムと物質生産

    吉田 健一

    日本生物工学会, 2012年10月, 生物工学会誌, 90 (10), 623 - 624, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 2Ap03 Bacillus subtilis cell factory for production of scyllo-inositol promising for Alzheimer's disease (Bio-Based Production)

    YOSHIDA Ken-ichi, ONUMA Chumsakul, ISHIKAWA Shu, OGASAWARA Naotake

    公益社団法人日本生物工学会, 2012年10月, 日本生物工学会大会講演要旨集, 64, 英語

    講演資料等(セミナー,チュートリアル,講習,講義他)

  • 大豆(ダイズ)の新規機能性成分開発

    吉田 健一

    2012年02月, 月刊バイオインダストリー, 29 (2), 15 - 20, 日本語

    [招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産

    吉田 健一

    2011年10月, 生物工学会誌, 89 (10), 585 - 588, 日本語

    [査読有り][招待有り]

    記事・総説・解説・論説等(学術雑誌)

  • 枯草菌のdegU32(hy)はカタボライトレプレッションを積極的に解除する

    IWASAKI Kana, ISHIKAWA Shu, OGASAWARA Naotake, TAKENAKA Shinji, YOSHIDA Ken‐Ichi

    2011年, 日本分子生物学会年会プログラム・要旨集(Web), 34th, 2P - 0003 (WEB ONLY), 日本語

    講演資料等(セミナー,チュートリアル,講習,講義他)

  • Itsuko Fukuda, Rie Mukai, Masaya Kawase, Ken-ichi Yoshida, Hitoshi Ashida

    Flavonoids have been reported to be dietary antagonists of an aryl hydrocarbon receptor (AhR). However, little is known about the molecular mechanism on their antagonistic effects. In this study, the inhibitory effect of flavonoids on ligand binding to the AhR and interaction between flavonoids and the AhR complex (AhRc) were investigated in each flavonoid subclass. Flavone, flavonol, and flavanone but not catechin inhibited the specific binding between the AhR and 3-methylcholanthrene dose-dependently, indicating that the former three subclasses possibly act as competitive antagonists of the AhR. However, catechin in addition to the former three subclasses directly interacted with the AhRc by surface plasmon resonance analysis. The dissociation constant values showed an inverse correlation with the suppressive effect on the DNA binding activity. These results suggest that flavone, flavonol, and flavanone act as competitive antagonists of the AhR, while catechin associates with the AhRc and indirectly exhibits its antagonistic effects. (c) 2007 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2007年08月, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 359 (3), 822 - 827, 英語

  • Itsuko Fukuda, Rie Mukai, Masaya Kawase, Ken-ichi Yoshida, Hitoshi Ashida

    Flavonoids have been reported to be dietary antagonists of an aryl hydrocarbon receptor (AhR). However, little is known about the molecular mechanism on their antagonistic effects. In this study, the inhibitory effect of flavonoids on ligand binding to the AhR and interaction between flavonoids and the AhR complex (AhRc) were investigated in each flavonoid subclass. Flavone, flavonol, and flavanone but not catechin inhibited the specific binding between the AhR and 3-methylcholanthrene dose-dependently, indicating that the former three subclasses possibly act as competitive antagonists of the AhR. However, catechin in addition to the former three subclasses directly interacted with the AhRc by surface plasmon resonance analysis. The dissociation constant values showed an inverse correlation with the suppressive effect on the DNA binding activity. These results suggest that flavone, flavonol, and flavanone act as competitive antagonists of the AhR, while catechin associates with the AhRc and indirectly exhibits its antagonistic effects. (c) 2007 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2007年08月, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 359 (3), 822 - 827, 英語

  • A holistic view of inositol catabolism in Bacillus subtilis

    吉田 健一

    2007年01月, Global Regulatory Networks in Bacillus subtilis, 75-90, 英語

    記事・総説・解説・論説等(学術雑誌)

  • Ken-ichi Yoshida, Won-Seok Kim, Masaki Kinehara, Rie Mukai, Hitoshi Ashida, Hideki Ikeda, Yasutaro Fujita, Hari B. Krishnan

    Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently WE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An WE ortholog was cloned from USDA191. USDA191 WE was expressed in Escherichia coli as a His(6)-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 WE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis WE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myoinositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and WE.

    TAYLOR & FRANCIS LTD, 2006年12月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (12), 2957 - 2964, 英語

  • Ken-ichi Yoshida, Won-Seok Kim, Masaki Kinehara, Rie Mukai, Hitoshi Ashida, Hideki Ikeda, Yasutaro Fujita, Hari B. Krishnan

    Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently WE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An WE ortholog was cloned from USDA191. USDA191 WE was expressed in Escherichia coli as a His(6)-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 WE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis WE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myoinositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and WE.

    TAYLOR & FRANCIS LTD, 2006年12月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (12), 2957 - 2964, 英語

  • Tetsuro Morinaga, Masanori Yamaguchi, Yuki Makino, Hideaki Nanamiya, Kiwamu Takahashi, Hirofumi Yoshikawa, Fujio Kawamura, Hitoshi Ashida, Ken-ichi Yoshida

    Soybeans are rich in pinitol (PI; 3-O-methyl-D-chiroinositol), which improves health by treating conditions associated with insulin resistance, such as diabetes mellitus and obesity. Natto is a food made from soybeans fermented by strains of Bacillus subtilis natto. In the chromosome of natto strain OK2, there is a putative promoter region almost identical to the iol promoter for myo-inositol (MI) catabolic genes of B. subtilis 168. In the presence of MI, the putative iol promoter functioned to induce inositol dehydrogenase, the enzyme for the first-step reaction in the MI catabolic pathway. PI also induced inositol dehydrogenase and the promoter was indispensable for the utilization of PI as well as MI, suggesting that PI might be an alternative carbon source metabolized in a way involving the MI catabolic genes. Natto fermentation studies have revealed that the parental natto strain consumed PI while a mutant defective in the iol promoter did not do so at all. These results suggest that inactivating the MI catabolic genes might prevent PI consumption, retaining it in natto for enrichment of possible health-promoting properties.

    TAYLOR & FRANCIS LTD, 2006年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (8), 1913 - 1920, 英語

  • Tetsuro Morinaga, Masanori Yamaguchi, Yuki Makino, Hideaki Nanamiya, Kiwamu Takahashi, Hirofumi Yoshikawa, Fujio Kawamura, Hitoshi Ashida, Ken-ichi Yoshida

    Soybeans are rich in pinitol (PI; 3-O-methyl-D-chiroinositol), which improves health by treating conditions associated with insulin resistance, such as diabetes mellitus and obesity. Natto is a food made from soybeans fermented by strains of Bacillus subtilis natto. In the chromosome of natto strain OK2, there is a putative promoter region almost identical to the iol promoter for myo-inositol (MI) catabolic genes of B. subtilis 168. In the presence of MI, the putative iol promoter functioned to induce inositol dehydrogenase, the enzyme for the first-step reaction in the MI catabolic pathway. PI also induced inositol dehydrogenase and the promoter was indispensable for the utilization of PI as well as MI, suggesting that PI might be an alternative carbon source metabolized in a way involving the MI catabolic genes. Natto fermentation studies have revealed that the parental natto strain consumed PI while a mutant defective in the iol promoter did not do so at all. These results suggest that inactivating the MI catabolic genes might prevent PI consumption, retaining it in natto for enrichment of possible health-promoting properties.

    TAYLOR & FRANCIS LTD, 2006年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (8), 1913 - 1920, 英語

  • S Nishiumi, Y Yabushita, Fukuda, I, R Mukai, KI Yoshida, H Ashida

    Dioxins enter the body mainly through diet and cause the various toxicological effects by binding to the cytosolic aryl hydrocarbon receptor (AhR) followed by its transformation. In recent reports, it has been shown that certain natural compounds suppress AhR transformation in vitro. In this study, we demonstrated that ethanolic extract from molokhia, known as Egyptian spinach, showed the strongest suppressive effect on AhR transformation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in cell-free system using rat hepatic cytosol among 41 kinds of extracts from vegetables and fruits. The molokhia extract also suppressed TCDD-induced AhR transformation in mouse hepatoma Hepa-1c1c7 cells and in intestinal permeability system constructed with human colon adenocarcinoma Caco-2 cells and human hepatoma HepG2 cells. Moreover, oral administration of the molokhia extract (100 mg/kg body weight) decreased 3-methylcholanthrene-induced AhR transformation to the control level by inhibiting translocation of the AhR from cytosol into the nucleus in the liver of rats. The molokhia extract-administered rat liver showed a tolerance to TCDD-induced AhR transformation by ex vivo experiment. These results indicate that molokhia is an attractive food for isolation and identification of a natural antagonist for the AhR. (c) 2005 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2006年02月, FOOD AND CHEMICAL TOXICOLOGY, 44 (2), 250 - 260, 英語

  • S Nishiumi, Y Yabushita, Fukuda, I, R Mukai, KI Yoshida, H Ashida

    Dioxins enter the body mainly through diet and cause the various toxicological effects by binding to the cytosolic aryl hydrocarbon receptor (AhR) followed by its transformation. In recent reports, it has been shown that certain natural compounds suppress AhR transformation in vitro. In this study, we demonstrated that ethanolic extract from molokhia, known as Egyptian spinach, showed the strongest suppressive effect on AhR transformation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in cell-free system using rat hepatic cytosol among 41 kinds of extracts from vegetables and fruits. The molokhia extract also suppressed TCDD-induced AhR transformation in mouse hepatoma Hepa-1c1c7 cells and in intestinal permeability system constructed with human colon adenocarcinoma Caco-2 cells and human hepatoma HepG2 cells. Moreover, oral administration of the molokhia extract (100 mg/kg body weight) decreased 3-methylcholanthrene-induced AhR transformation to the control level by inhibiting translocation of the AhR from cytosol into the nucleus in the liver of rats. The molokhia extract-administered rat liver showed a tolerance to TCDD-induced AhR transformation by ex vivo experiment. These results indicate that molokhia is an attractive food for isolation and identification of a natural antagonist for the AhR. (c) 2005 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2006年02月, FOOD AND CHEMICAL TOXICOLOGY, 44 (2), 250 - 260, 英語

  • HARVIE Duncan R, ANDREINI Claudia, CAVALLARO Gabriele, MENG Wenmao, CONNOLLY Bernard A, YOSHIDA Ken‐ichi, FUJITA Yasutaro, HARWOOD Colin R, RADFORD David S, TOTTEY Stephen, CAVET Jennifer S, ROBINSON Nigel J

    2006年, Mol Microbiol, 59 (4), 1341 - 1356

  • HARVIE Duncan R, ANDREINI Claudia, CAVALLARO Gabriele, MENG Wenmao, CONNOLLY Bernard A, YOSHIDA Ken‐ichi, FUJITA Yasutaro, HARWOOD Colin R, RADFORD David S, TOTTEY Stephen, CAVET Jennifer S, ROBINSON Nigel J

    2006年, Mol Microbiol, 59 (4), 1341 - 1356

  • 枯草菌のイノシトール分解系―機能解明とその応用の可能性―

    吉田 健一

    2005年09月, 化学と生物, 43 9 566-568, 日本語

    記事・総説・解説・論説等(学術雑誌)

  • 今日の話題「枯草菌のイノシトール分解系-機能解明とその応用の可能性-

    吉田 健一

    2005年, 化学と生物, 43 9 566-568, 日本語

    [査読有り]

    その他

  • YOSHIDA Ken‐ichi, YAMAGUCHI Masanori, MORINAGA Tetsuro, IKEUCHI Maya, KINEHARA Masaki, ASHIDA Hitoshi

    2005年, Appl Environ Microbiol, 72 (2), 1310 - 1315

  • YOSHIDA Ken‐ichi, YAMAGUCHI Masanori, MORINAGA Tetsuro, IKEUCHI Maya, KINEHARA Masaki, ASHIDA Hitoshi

    2005年, Appl Environ Microbiol, 72 (2), 1310 - 1315

  • S Tojo, T Satomura, K Morisaki, K Yoshida, K Hirooka, Y Fujita

    The Bacillus subtilis ilv-leu operon is involved in the synthesis of branched-chain amino acids (valine, isoleucine, and leucine). The two- to threefold repression of expression of the ilv-leu operon during logarithmic-phase growth under nitrogen-limited conditions, which was originally detected by a DNA microarray analysis to compare the transcriptomes from the wild-type and tnrA mutant strains, was confirmed by lacZ fusion and Northern experiments. A genome-wide TnrA box search revealed a candidate box approximately 200 bp upstream of the transcription initiation base of the ilv-leu operon, the TnrA binding to which was verified by gel retardation and DNase I footprinting analyses. Deletion and base substitution of the TnrA box sequence affected the ilv-leu promoter activity in vivo, implying that TnrA bound to the box might be able to inhibit the promoter activity, possibly through DNA bending. The negative control of the expression of the ilv-leu operon by TnrA, which is considered to represent rather fine-tuning (two- to threefold), is a novel regulatory link between nitrogen and amino acid metabolism.

    AMER SOC MICROBIOLOGY, 2004年12月, JOURNAL OF BACTERIOLOGY, 186 (23), 7971 - 7979, 英語

  • S Tojo, T Satomura, K Morisaki, K Yoshida, K Hirooka, Y Fujita

    The Bacillus subtilis ilv-leu operon is involved in the synthesis of branched-chain amino acids (valine, isoleucine, and leucine). The two- to threefold repression of expression of the ilv-leu operon during logarithmic-phase growth under nitrogen-limited conditions, which was originally detected by a DNA microarray analysis to compare the transcriptomes from the wild-type and tnrA mutant strains, was confirmed by lacZ fusion and Northern experiments. A genome-wide TnrA box search revealed a candidate box approximately 200 bp upstream of the transcription initiation base of the ilv-leu operon, the TnrA binding to which was verified by gel retardation and DNase I footprinting analyses. Deletion and base substitution of the TnrA box sequence affected the ilv-leu promoter activity in vivo, implying that TnrA bound to the box might be able to inhibit the promoter activity, possibly through DNA bending. The negative control of the expression of the ilv-leu operon by TnrA, which is considered to represent rather fine-tuning (two- to threefold), is a novel regulatory link between nitrogen and amino acid metabolism.

    AMER SOC MICROBIOLOGY, 2004年12月, JOURNAL OF BACTERIOLOGY, 186 (23), 7971 - 7979, 英語

  • K Yoshida, YH Ohki, M Murata, M Kinehara, H Matsuoka, T Satomura, R Ohki, M Kumano, K Yamane, Y Fujita

    The Bacillus subtilis lmrAB operon is involved in multidrug resistance. LmrA is a repressor of its own operon, while LmrB acts as a multidrug efflux transporter. LmrA was produced in Escherichia coli cells and was shown to bind to the lmr promoter region, in which an LmrA-binding site was identified. Genome-wide screening involving DNA microarray analysis allowed us to conclude that LmrA also repressed yxaGH, which was not likely to contribute to the multidrug resistance. LmrA bound to a putative yxaGH promoter region, in which two tandem LmrA-binding sites were identified. The LmrA regulon was thus determined to comprise lmrAB and yxaGH. All three LmrA-binding sites contained an 18-bp consensus sequence, TAGACCRKTCWMTATA WT, which could play an important role in LmrA binding.

    AMER SOC MICROBIOLOGY, 2004年09月, JOURNAL OF BACTERIOLOGY, 186 (17), 5640 - 5648, 英語

  • K Yoshida, YH Ohki, M Murata, M Kinehara, H Matsuoka, T Satomura, R Ohki, M Kumano, K Yamane, Y Fujita

    The Bacillus subtilis lmrAB operon is involved in multidrug resistance. LmrA is a repressor of its own operon, while LmrB acts as a multidrug efflux transporter. LmrA was produced in Escherichia coli cells and was shown to bind to the lmr promoter region, in which an LmrA-binding site was identified. Genome-wide screening involving DNA microarray analysis allowed us to conclude that LmrA also repressed yxaGH, which was not likely to contribute to the multidrug resistance. LmrA bound to a putative yxaGH promoter region, in which two tandem LmrA-binding sites were identified. The LmrA regulon was thus determined to comprise lmrAB and yxaGH. All three LmrA-binding sites contained an 18-bp consensus sequence, TAGACCRKTCWMTATA WT, which could play an important role in LmrA binding.

    AMER SOC MICROBIOLOGY, 2004年09月, JOURNAL OF BACTERIOLOGY, 186 (17), 5640 - 5648, 英語

  • KI Yoshida, M Yamaguchi, H Ikeda, K Omae, KI Tsurusaki, Y Fujita

    The myo-inositol catabolism pathway of Bacillus subtilis has not been fully characterized but was proposed to involve step-wise multiple reactions that finally yielded acetyl-CoA and dihydroxyacetone phosphate. It is known that the iolABCDEFGHIJ operon is responsible for the catabolism of inositol. IoIG catalyses the first step of myo-inositol catabolism, the dehydrogenation of myo-inositol, producing 2-keto-myo-inositol (inosose). The second step was thought to be the dehydration of inosose. Genetic and biochemical analyses of the iol genes led to the identification of iolE, encoding the enzyme for the second step of inositol catabolism, inosose dehydratase. The reaction product of inosose dehydratase was identified as D-2,3-diketo-4-deoxy-epi-inositol.

    SOC GENERAL MICROBIOLOGY, 2004年03月, MICROBIOLOGY-SGM, 150 (3), 571 - 580, 英語

  • Ken-Ichi Yoshida, Masanori Yamaguchi, Hideki Ikeda, Kaoru Omae, Ken-Ichi Tsurusaki, Yasutaro Fujita

    The myo-inositol catabolism pathway of Bacillus subtilis has not been fully characterized but was proposed to involve step-wise multiple reactions that finally yielded acetyl-CoA and dihydroxyacetone phosphate. It is known that the iolABCDEFGHIJ operon is responsible for the catabolism of inositol. IolG catalyses the first step of myo-inositol catabolism, the dehydrogenation of myo-inositol, producing 2-keto-myo-inositol (inosose). The second step was thought to be the dehydration of inosose. Genetic and biochemical analyses of the iol genes led to the identification of iolE, encoding the enzyme for the second step of inositol catabolism, inosose dehydratase. The reaction product of inosose dehydratase was identified as D-2,3-diketo-4-deoxy-epi-inositol.

    2004年03月, Microbiology (Reading, England), 150 (Pt 3), 571 - 580, 英語, 国際誌

    [査読有り]

  • モデル微生物としての枯草菌

    吉田 健一

    2004年, 月刊海洋, 36(8) 579-587, 日本語

    その他

  • Black tea (Camellia sinensis) suppresses hyperglycemia in STZ-induced diabetic rats

    2004年, Proceedings of 2004 International Conference on O-CHA (tea) Culture and Science, 561-562

  • S Tojo, M Matsunaga, T Matsumoto, CM Kang, H Yamaguchi, K Asai, Y Sadaie, K Yoshida, Y Fujita

    We investigated the organization and expression of the Bacillus subtilis sigY operon, the first gene of which codes for sigma(Y), a member of the extracytoplasmic function (ECF) family of sigma factors. The sigY operon, comprising six genes (sigY, yxlC, D, E, F, and G), was induced upon nitrogen starvation; it was continuously transcribed from the 31st base upstream of sigY to a neighboring convergent gene, yxlH, resulting in a 4.2-kb mRNA. The expression of the sigY operon was also positively autoregulated through sigma(Y), suggesting that its transcription is likely to be directed by sigma(Y). Deletion analysis of the sigY promoter, which was localized by primer extension, revealed the promoter region of sigY with the "-10" and "-35" sequences of CGTC and TGAACG, respectively. The latter sequence was distinct from those recognized by sigma(W), sigma(X), and sigma(M). The ay-directed transcription of sigY was under negative regulation involving Yx1D. sigY disruption affected sporulation induced by nitrogen starvation, but sigY induction upon nitrogen starvation was not associated with the sporulation process. The organization and function of the sigY operon are significantly conserved in several microorganisms living in adverse living environments.

    JAPANESE BIOCHEMICAL SOC, 2003年12月, JOURNAL OF BIOCHEMISTRY, 134 (6), 935 - 946, 英語

  • S Tojo, M Matsunaga, T Matsumoto, CM Kang, H Yamaguchi, K Asai, Y Sadaie, K Yoshida, Y Fujita

    We investigated the organization and expression of the Bacillus subtilis sigY operon, the first gene of which codes for sigma(Y), a member of the extracytoplasmic function (ECF) family of sigma factors. The sigY operon, comprising six genes (sigY, yxlC, D, E, F, and G), was induced upon nitrogen starvation; it was continuously transcribed from the 31st base upstream of sigY to a neighboring convergent gene, yxlH, resulting in a 4.2-kb mRNA. The expression of the sigY operon was also positively autoregulated through sigma(Y), suggesting that its transcription is likely to be directed by sigma(Y). Deletion analysis of the sigY promoter, which was localized by primer extension, revealed the promoter region of sigY with the "-10" and "-35" sequences of CGTC and TGAACG, respectively. The latter sequence was distinct from those recognized by sigma(W), sigma(X), and sigma(M). The ay-directed transcription of sigY was under negative regulation involving Yx1D. sigY disruption affected sporulation induced by nitrogen starvation, but sigY induction upon nitrogen starvation was not associated with the sporulation process. The organization and function of the sigY operon are significantly conserved in several microorganisms living in adverse living environments.

    JAPANESE BIOCHEMICAL SOC, 2003年12月, JOURNAL OF BIOCHEMISTRY, 134 (6), 935 - 946, 英語

  • T Doan, P Servant, S Tojo, H Yamaguchi, G Lerondel, K Yoshida, Y Fujita, S Aymerich

    A transcriptome comparison of a wild-type Bacillus subtilis strain growing under glycolytic or gluconeogenic conditions was performed. In particular, it revealed that the ywkA gene, one of the four paralogues putatively encoding a malic, enzyme, was more transcribed during gluconeogenesis. Using a lacZ reporter fusion to the ywkA promoter, it was shown that ywkA was specifically induced by external malate and not subject to glucose catabolite repression. Northern analysis confirmed this expression pattern and demonstrated that ywkA is cotranscribed with the downstream ywkB gene. The ywkA gene product was purified and biochemical studies demonstrated its malic enzyme activity, which was 10-fold higher with NAD than with NADP (k(cat)/K-m 102 and 10 s(-1) mM(-1), respectively). However, physiological tests with single and multiple mutant strains affected in ywkA and/or in ywkA paralogues showed that ywkA does not contribute to efficient utilization of malate for growth. Transposon mutagenesis allowed the identification of the uncharacterized YufL/YufM two-component system as being responsible for the control of ywkA expression. Genetic analysis and in vitro studies with purified YufM protein showed that YufM binds just upstream of ywkA promoter and activates ywkA transcription in response to the presence of malate in the extracellular medium, transmitted by YufL. ywkA and yufL/yufM could thus be renamed maeA for malic enzyme and malK/malR for malate kinase sensor/malate response regulator, respectively.

    SOC GENERAL MICROBIOLOGY, 2003年09月, MICROBIOLOGY-SGM, 149 (9), 2331 - 2343, 英語

  • T Doan, P Servant, S Tojo, H Yamaguchi, G Lerondel, K Yoshida, Y Fujita, S Aymerich

    A transcriptome comparison of a wild-type Bacillus subtilis strain growing under glycolytic or gluconeogenic conditions was performed. In particular, it revealed that the ywkA gene, one of the four paralogues putatively encoding a malic, enzyme, was more transcribed during gluconeogenesis. Using a lacZ reporter fusion to the ywkA promoter, it was shown that ywkA was specifically induced by external malate and not subject to glucose catabolite repression. Northern analysis confirmed this expression pattern and demonstrated that ywkA is cotranscribed with the downstream ywkB gene. The ywkA gene product was purified and biochemical studies demonstrated its malic enzyme activity, which was 10-fold higher with NAD than with NADP (k(cat)/K-m 102 and 10 s(-1) mM(-1), respectively). However, physiological tests with single and multiple mutant strains affected in ywkA and/or in ywkA paralogues showed that ywkA does not contribute to efficient utilization of malate for growth. Transposon mutagenesis allowed the identification of the uncharacterized YufL/YufM two-component system as being responsible for the control of ywkA expression. Genetic analysis and in vitro studies with purified YufM protein showed that YufM binds just upstream of ywkA promoter and activates ywkA transcription in response to the presence of malate in the extracellular medium, transmitted by YufL. ywkA and yufL/yufM could thus be renamed maeA for malic enzyme and malK/malR for malate kinase sensor/malate response regulator, respectively.

    SOC GENERAL MICROBIOLOGY, 2003年09月, MICROBIOLOGY-SGM, 149 (9), 2331 - 2343, 英語

  • K Yoshida, H Yamaguchi, M Kinehara, Y Ohki, Y Nakaura, Y Fujita

    Bacillus subtilis TnrA is a global regulator that responds to the availability of nitrogen sources and both activates and represses many genes during nitrogen-limited growth. In order to obtain a holistic view of the gene regulation depending on TnrA, we performed a genome-wide screening for TnrA-regulated genes associated with a TnrA box. A combination of DNA microarray hybridization and a genome-wide search for TnrA boxes allowed us to find 36 TnrA-regulated transcription units associated with a putative TnrA box. Gel retardation assaying, using probes carrying at least one putative TnrA box and the deletion derivatives of each box, indicated that 17 out of 36 transcription units were likely TnrA targets associated with the TnrA boxes, two of which (nasA and nasBCDEF ) possessed a common TnrA box. The sequences of these TnrA boxes contained a consensus one, TGTNANAWWWTMTNACA. The TnrA targets detected in this study were nrgAB , pucJKLM , glnQHMP , nasDEF , oppABCDF , nasA , nasBCDEF and ywrD for positive regulation, and gltAB , pel , ywdIJK , yycCB , yttA , yxkC , ywlFG , yodF and alsT for negative regulation, nrgAB and gltAB being well-studied TnrA targets. It was unexpected that the negatively regulated TnrA targets were as many as the positively regulated targets. The physiological role of the TnrA regulon is discussed.

    BLACKWELL PUBLISHING LTD, 2003年07月, MOLECULAR MICROBIOLOGY, 49 (1), 157 - 165, 英語

  • K Yoshida, H Yamaguchi, M Kinehara, Y Ohki, Y Nakaura, Y Fujita

    Bacillus subtilis TnrA is a global regulator that responds to the availability of nitrogen sources and both activates and represses many genes during nitrogen-limited growth. In order to obtain a holistic view of the gene regulation depending on TnrA, we performed a genome-wide screening for TnrA-regulated genes associated with a TnrA box. A combination of DNA microarray hybridization and a genome-wide search for TnrA boxes allowed us to find 36 TnrA-regulated transcription units associated with a putative TnrA box. Gel retardation assaying, using probes carrying at least one putative TnrA box and the deletion derivatives of each box, indicated that 17 out of 36 transcription units were likely TnrA targets associated with the TnrA boxes, two of which (nasA and nasBCDEF ) possessed a common TnrA box. The sequences of these TnrA boxes contained a consensus one, TGTNANAWWWTMTNACA. The TnrA targets detected in this study were nrgAB , pucJKLM , glnQHMP , nasDEF , oppABCDF , nasA , nasBCDEF and ywrD for positive regulation, and gltAB , pel , ywdIJK , yycCB , yttA , yxkC , ywlFG , yodF and alsT for negative regulation, nrgAB and gltAB being well-studied TnrA targets. It was unexpected that the negatively regulated TnrA targets were as many as the positively regulated targets. The physiological role of the TnrA regulon is discussed.

    BLACKWELL PUBLISHING LTD, 2003年07月, MOLECULAR MICROBIOLOGY, 49 (1), 157 - 165, 英語

  • K Kobayashi, SD Ehrlich, A Albertini, G Amati, KK Andersen, M Arnaud, K Asai, S Ashikaga, S Aymerich, P Bessieres, F Boland, SC Brignell, S Bron, K Bunai, J Chapuis, LC Christiansen, A Danchin, M Debarbouille, E Dervyn, E Deuerling, K Devine, SK Devine, O Dreesen, J Errington, S Fillinger, SJ Foster, Y Fujita, A Galizzi, R Gardan, C Eschevins, T Fukushima, K Haga, CR Harwood, M Hecker, D Hosoya, MF Hullo, H Kakeshita, D Karamata, Y Kasahara, F Kawamura, K Koga, P Koski, R Kuwana, D Imamura, M Ishimaru, S Ishikawa, Ishio, I, D Le Coq, A Masson, C Mauel, R Meima, RP Mellado, A Moir, S Moriya, E Nagakawa, H Nanamiya, S Nakai, P Nygaard, M Ogura, T Ohanan, M O'Reilly, M O'Rourke, Z Pragai, HM Pooley, G Rapoport, JP Rawlins, LA Rivas, C Rivolta, A Sadaie, Y Sadaie, M Sarvas, T Sato, HH Saxild, E Scanlan, W Schumann, JFML Seegers, J Sekiguchi, A Sekowska, SJ Seror, M Simon, P Stragier, R Studer, H Takamatsu, T Tanaka, M Takeuchi, HB Thomaides, Vagner, V, JM van Dijl, K Watabe, A Wipat, H Yamamoto, M Yamamoto, Y Yamamoto, K Yamane, K Yata, K Yoshida, H Yoshikawa, U Zuber, N Ogasawara

    To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximate to4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from bacteria with small genomes. Unexpectedly, most genes involved in the Embden-Meyerhof-Parnas pathway are essential. Identification of unknown and unexpected essential genes opens research avenues to better understanding of processes that sustain bacterial life.

    NATL ACAD SCIENCES, 2003年04月, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (8), 4678 - 4683, 英語

  • K Kobayashi, SD Ehrlich, A Albertini, G Amati, KK Andersen, M Arnaud, K Asai, S Ashikaga, S Aymerich, P Bessieres, F Boland, SC Brignell, S Bron, K Bunai, J Chapuis, LC Christiansen, A Danchin, M Debarbouille, E Dervyn, E Deuerling, K Devine, SK Devine, O Dreesen, J Errington, S Fillinger, SJ Foster, Y Fujita, A Galizzi, R Gardan, C Eschevins, T Fukushima, K Haga, CR Harwood, M Hecker, D Hosoya, MF Hullo, H Kakeshita, D Karamata, Y Kasahara, F Kawamura, K Koga, P Koski, R Kuwana, D Imamura, M Ishimaru, S Ishikawa, Ishio, I, D Le Coq, A Masson, C Mauel, R Meima, RP Mellado, A Moir, S Moriya, E Nagakawa, H Nanamiya, S Nakai, P Nygaard, M Ogura, T Ohanan, M O'Reilly, M O'Rourke, Z Pragai, HM Pooley, G Rapoport, JP Rawlins, LA Rivas, C Rivolta, A Sadaie, Y Sadaie, M Sarvas, T Sato, HH Saxild, E Scanlan, W Schumann, JFML Seegers, J Sekiguchi, A Sekowska, SJ Seror, M Simon, P Stragier, R Studer, H Takamatsu, T Tanaka, M Takeuchi, HB Thomaides, Vagner, V, JM van Dijl, K Watabe, A Wipat, H Yamamoto, M Yamamoto, Y Yamamoto, K Yamane, K Yata, K Yoshida, H Yoshikawa, U Zuber, N Ogasawara

    To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximate to4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from bacteria with small genomes. Unexpectedly, most genes involved in the Embden-Meyerhof-Parnas pathway are essential. Identification of unknown and unexpected essential genes opens research avenues to better understanding of processes that sustain bacterial life.

    NATL ACAD SCIENCES, 2003年04月, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (8), 4678 - 4683, 英語

  • K Asai, H Yamaguchi, CM Kang, K Yoshida, Y Fujita, Y Sadaie

    Target gene candidates of the seven extracytoplasmic function (ECF) sigma factors of Bacillus subtilis have been surveyed using DNA microarray analysis of mRNA extracted from cells grown in Luria-Bertani broth, in which an ECF sigma factor gene was placed under the control of the spac promoter on multicopy plasmid pDG148 and overexpressed. The number of target candidates for each of the sigma factors varied greatly. and a total of 278 genes were selected. Interestingly, the above target gene candidates shared only one gene out of 94 target genes of the general stress sigma B that have been reported in the literature thus far. Furthermore, lacZ-fusion experiments based on the results of DNA microarray analysis indicated that each ECF sigma factor directs transcription of its own operon, with the exception of sigZ. The DNA microarray data collected in this study are available at the KEGG Expression Database web site (http://www.genome.ad.jp/kegg/expression/). (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2003年03月, FEMS MICROBIOLOGY LETTERS, 220 (1), 155 - 160, 英語

  • K Asai, H Yamaguchi, CM Kang, K Yoshida, Y Fujita, Y Sadaie

    Target gene candidates of the seven extracytoplasmic function (ECF) sigma factors of Bacillus subtilis have been surveyed using DNA microarray analysis of mRNA extracted from cells grown in Luria-Bertani broth, in which an ECF sigma factor gene was placed under the control of the spac promoter on multicopy plasmid pDG148 and overexpressed. The number of target candidates for each of the sigma factors varied greatly. and a total of 278 genes were selected. Interestingly, the above target gene candidates shared only one gene out of 94 target genes of the general stress sigma B that have been reported in the literature thus far. Furthermore, lacZ-fusion experiments based on the results of DNA microarray analysis indicated that each ECF sigma factor directs transcription of its own operon, with the exception of sigZ. The DNA microarray data collected in this study are available at the KEGG Expression Database web site (http://www.genome.ad.jp/kegg/expression/). (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2003年03月, FEMS MICROBIOLOGY LETTERS, 220 (1), 155 - 160, 英語

  • 枯草菌のDNA マイクロアレイ解析

    山口 弘毅, 吉田 健一, 藤田 泰太郎

    2003年, 生化学, 75(5) 407-410, 日本語

    その他

  • 吉田健一

    2003年, 日本農芸化学会誌, 77 (1), 12 - 17

  • 尾前薫, 吉田健一, 鶴崎健一, 藤田泰太郎

    2001年03月05日, 日本農芸化学会誌, 75, 105, 日本語

  • Ishii, T, Yoshida, K, Terai, G, Fujita, Y, Nakai, K

    2001年, Nucleic Acids Res., 29 (1), 278 - 280

    [査読有り]

  • 吉田健一, 尾前薫, 藤田泰太郎

    2000年11月25日, 日本分子生物学会年会プログラム・講演要旨集, 23rd, 450, 日本語

  • MA Petit, K Yoshida, Y Fujita, SD Ehrlich

    SOC GENERAL MICROBIOLOGY, 2000年09月, MICROBIOLOGY-UK, 146, 2091 - 2092, 英語

    [査読有り]

    速報,短報,研究ノート等(学術雑誌)

  • K Yoshida, Ishio, I, E Nagakawa, Y Yamamoto, M Yamamoto, Y Fujita

    Within the framework of the international project 'The functional analysis of the Bacillus subtilis genome' in Japan and Europe, the gene expression and transcription organization of the gntZ-ywaA region (160 kb) of the B. subtilis genome has been systematically analysed. First, all unanalysed genes comprising more than 80 amino acids (125 genes) in this region were inactivated through integration of plasmid pMUTIN. No essential gene was found which could not be inactivated. All the integrants grew normally in both nutrient sporulation medium and glucose minimal medium. But an integrant in the yxbG gene exhibited an oligosporogenic phenotype in the nutrient sporulation medium. The synthesis of beta-galactosidase was examined, as a reporter for expression of the inactivated genes, during growth and sporulation in the two media. The results indicated that 36% of the promoters were inactive when cells were grown in at least one of these two media. Furthermore, the transcription of the 119 genes in this region was analysed by Northern blotting, resulting in a transcription map. The results indicate that the gntZ-ywaA region contains at least 24 polycistronic operons, including several published ones. The operons newly found in this work are yxaAB, yxaGH, yxaJKL, yxbBA-yxnB-asnH-yxaM. yxbCD, yxcED, yxdJK, yxeFGH, yxeKLMNOPQ, yxeR-yxxB, hutPHUIGM, bgIPH-yxiE, wapA-yxxG, yxiM-deaD, katB-yxiS, yxjCDEF, yxjJI and yxkF-mmsX.

    SOC GENERAL MICROBIOLOGY, 2000年03月, MICROBIOLOGY-UK, 146 (3), 573 - 579, 英語

    [査読有り]

  • Yoshida. K, Fujita, Y, Ehrlich, S

    2000年, J.Bacteriol., 182 (19), 5454 - 5461

    [査読有り]

  • KI Yoshida, Y Fujita, SD Ehrlich

    Three asparagine synthetase genes, asnB, asnH, and asnO (yisQ), were predicted from the sequence of the Bacillus subtilis genome. We show here that the three genes are expressed differentially during cell growth. In a rich sporulation medium, expression of asnB was detected only during exponential growth, that of asnH was drastically elevated at the transition between exponential growth and stationary phase, and that of asnO was seen only later in sporulation. In a minimal medium, both asnB and asnH were expressed constitutively during exponential growth and in stationary phase, while the expression of asnO was not detected in either phase. However, when the minimal medium was supplemented with asparagine, only the expression of asnH was partially repressed. Transcription analyses revealed that asnB was possibly cotranscribed with a downstream gene, ytnA, while the asnH gene was transcribed as the fourth gene of an operon comprising yxbB, yxbA, yxnB, asnH, and yxaM. The asnO gene is a monocistronic operon, the expression of which was dependent on one of the sporulation sigma factors, sigma-E. Each of the three genes, carried on a low-copy-number plasmid, complemented the asparagine deficiency of an Escherichia coli strain lacking asparagine synthetases, indicating that all encode an asparagine synthetase. In B. subtilis, deletion of asnO or asnH, singly or in combination, had essentially no effect on growth rates in media with or without asparagine. In contrast, deletion of asnB led to a slow-growth phenotype, even in the presence of asparagine. A strain lacking all three genes still grew without asparagine, albeit very slowly, implying that B. subtilis might have yet another asparagine synthetase, not recognized by sequence analysis. The strains lacking asnO failed to sporulate, indicating an involvement of this gene in sporulation.

    AMER SOC MICROBIOLOGY, 1999年10月, JOURNAL OF BACTERIOLOGY, 181 (19), 6081 - 6091, 英語

    [査読有り]

  • Yoshida, K, Shibayama, T, Aoyama, D, Fujita, Y

    1999年, J. Mol.Biol., 285 (3), 917 - 929

    [査読有り]

  • 小笠原直毅, 定家義人, 藤田昌也, 吉田健一, 藤田泰太郎, 吉川博文, 三輪泰彦, 山本博規, 関口順一, 熊野みゆき, 山根國男, 村田麻喜子, 大木玲子

    1999年, 蛋白質 核酸 酵素, 44, 1449 - 1459

  • Y Fujita, KI Yoshida, Y Miwa, N Yanai, E Nagakawa, Y Kasahara

    The Bacillus subtilis fbp gene encoding fructose-1,6-bisphosphatase (FBPase) was originally identified as yydE. The fbp gene was expressed at a fairly constant level in cells undergoing glycolysis or gluconeogenesis,fbp transcription was initiated 94 bp upstream of the translation initiation codon, resulting in a 2.4-kb monocistronic transcript. Interestingly, B. subtilis FBPase exhibited no significant similarity to other FBPases in protein sequence databases.

    AMER SOC MICROBIOLOGY, 1998年08月, JOURNAL OF BACTERIOLOGY, 180 (16), 4309 - 4313, 英語

    [査読有り]

  • K Yoshida, K Shindo, H Sano, S Seki, M Fujimura, N Yanai, Y Miwa, Y Fujita

    Within the framework of an international project for the sequencing of the entire Bacillus subtilis genome, this paper communicates the sequencing of a chromosome region containing the lic and cel loci (65 kb), which creates a 177 kb contig covering the region from got to sacXY. This 65 kb region contains 64 ORFs (62 complete and two partial genes). The 14th, 15th and 17th genes correspond to licT, licS and katE, encoding the antiterminator for licS transcription, beta-glucanase (lichenase) and catalase 2, respectively. The 11th, 30th, 36th, 39th, 41st, 45th-48th, 51st and 58th genes are designated deaD, pepT, galE, aldY, msmX, cydABCD, sigY and katX because their products probably encode ATP-dependent RNA helicase, tripeptidase, UDP-glucose 4-epimerase, aldehyde dehydrogenase, multiple sugar-binding transport ATP-binding protein, the respective components of cytochrome d ubiquinol oxidase and ATP-binding cassette transporter, sigma-factor of RNA polymerase and catalase, respectively. The 60th-64th genes are celRABCD, which are probably involved in cellobiose utilization. Gene organization and gene features in the gnt-sacXY region are discussed.

    SOC GENERAL MICROBIOLOGY, 1996年11月, MICROBIOLOGY-UK, 142 (11), 3113 - 3123, 英語

    [査読有り]

  • K YOSHIDA, H SANO, S SEKI, M ODA, M FUJIMURA, Y FUJITA

    Within the framework of an international project for the sequencing of the entire Bacillus subtilis genome, a 29 kb chromosome segment, which contains the hut operon (335 degrees) and the wapA gene, has been cloned and sequenced, This region (28954 bp) contains 21 complete ORFs and one partial one. The 5th, 6th and 17th genes correspond to hutH encoding histidase, hutP encoding the positive regulator for the hut operon and wapA encoding a precursor of three major wall-associated proteins, respectively. A homology search for their products deduced from the 21 complete ORFs revealed that nine of them exhibit significant homology to known proteins such as urocanase (Pseudomonas putida), a protein involved in clavulanic acid biosynthesis (Streptomyces griseus), amino acid permeases (lysine, Escherichia coli; histidine, Saccharomyces cerevisiae; and others), beta-glucoside-specific phosphotransferases (E. coli and Erwinia chrysanthemi) and 6-phospho-beta-glucosidases (E. coli and Erw. chrysanthemi). Based on the features of the determined sequence and the results of the homology search, as well as on genetic data and sequence of the hut genes reported by other groups, it is predicted that the B. subtilis hut operon may consist of the following six genes (6th-1st), the last of which is followed by a typical p-independent transcription terminator: hutP, hutH, EE57A (hutU) encoding urocanase, EE57B (hutI) encoding imidazolone-5-propionate hydrolase, EE57C (hutG) encoding formiminoglutamate hydrolase and EE57D (tentatively designated as hutM) possibly encoding histidine permease. Interestingly, the direction of transcription of these hut genes is opposite to that of the movement of the replication fork.

    SOC GENERAL MICROBIOLOGY, 1995年02月, MICROBIOLOGY-UK, 141 (2), 337 - 343, 英語

    [査読有り]

  • Yoshida, K, Ohmori, H, Miwa, Y, Fujita, Y

    1995年, J. Bacteriol., 117 (16), 4813 - 4816

    [査読有り]

  • Yoshida, K, Seki, S, Fujimura, M, Miwa, Y, Fujita, Y

    1995年, DNA Res., 2 (2), 61 - 69

    [査読有り]

  • Yoshida, K, Fujimura, M, Yanai, N, Fujita, Y

    1995年, DNA Res., 2 (6), 295 - 301

    [査読有り]

  • Yoshida, K, Sano, H, Miwa, Y, Ogasawara, N, Fujita, Y

    1994年, Microbiology, 140 (19), 2289 - 2298

    [査読有り]

  • Yoshida, K, Seki, S, Fujita, Y

    1994年, DNA Res., 1 (4), 157 - 162

    [査読有り]

  • K YOSHIDA, Y FUJITA, E MUKAI, K SEN, M HIMENO, H SAKAI, T KOMANO

    TAYLOR & FRANCIS LTD, 1993年07月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 57 (7), 1200 - 1201, 英語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • K YOSHIDA, Y FUJITA, E MUKAI, K SEN, M HIMENO, H SAKAI, T KOMANO

    To delineate the mosquitocidal regions of the ISRH3 (CryIVB) and ISRH4 (CryIVA) proteins, which are two of the mosquitocidal 130-kDa proteins contained in the crystalline protein bodies (CPBs) of Bacillus thuringiensis var. israelensis (BTI), a deletion analysis of these protein genes has been done. Based on the evidence that each 130-kDa protein had two mosquitocidal regions, N-terminal and C-terminal ones, and these two regions shared a common part in the center of the 130-kDa proteins, deleted genes on this region were constructed. As the protein products which lacked the central region had reduced activities, the central region could be important for the mosquitocidal activity. The mosquitocidal and non-mosquitocidal truncated gene products of 130-kDa protein genes were also applied to a cultured lepidopteran cell line, TN-368. The mosquitocidal proteins caused the swelling and disruption of the cells in spite of the insecticidal specificity of CPBs of BTI, but the non-mosquitocidal proteins did not. Therefore, TN-368 cells were sensitive to the mosquitocidal fragments of 130-kDa proteins of BTI under the assay conditions used.

    TAYLOR & FRANCIS LTD, 1993年04月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 57 (4), 584 - 590, 英語

    [査読有り]

  • Yoshida, K, Fujita, Y, Sarai, A

    1993年, J. Mol. Biol., 231 (2), 167 - 174

    [査読有り]

  • Y FUJITA, K SHINDO, Y MIWA, K YOSHIDA

    The Bacillus subtilis inositol dehydrogenase (Idh)-encoding gene (idh) was cloned in the B. subtilis temperate phage, rho-11, and then in Escherichia coli plasmids (pBR322 and pUC118). The nucleotide sequence of the idh gene, which consists of 344 codons and whose product has an M(r) of 38 351, was determined. E. coli, bearing pIOL05d15, in which expression of the idh gene is under the control of the lac promoter of pUC118, overproduced an active Idh to approx. 20% of total protein upon addition of isopropyl-beta-D-thiogalactopyranoside. This overproduced enzyme cross-reacted with an anti-Idh antibody, and exhibited the same M(r) and substrate specificity as those of the B. subtilis enzyme.

    ELSEVIER SCIENCE BV, 1991年12月, GENE, 108 (1), 121 - 125, 英語

    [査読有り]

書籍等出版物

  • Escherichia coli and Bacillus subtilis; the frontiers of molecular microbiology revisited

    吉田 健一

    共著, Research Signpost, 2012年10月, 英語

    教科書・概説・概論

  • 遺伝子工学と未来社会 「遺伝子工学」

    吉田 健一

    共著, 化学同人, 2012年03月, 日本語

    教科書・概説・概論

  • バイオコンバージョンによって効率的にビタミンを作る-イノシトール 「微生物機能学」

    吉田 健一

    共著, 三共出版, 2012年03月, 日本語

    教科書・概説・概論

  • Inositol derivatives stimulate glucose transport in muscle cells, in “Animal Cell Technology: Basic & Applied Aspects, Vol. 15, Eds. by, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya and Sanetaka Shirahata, pp. 225-231.

    YAP Angeline, NISHIUMI Shin, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    共著, Springer, 2011年, 英語

    学術書

  • Insulin-mimetic activity of inositol derivatives depends on phosphorylation of PKC ζ

    DANG Thuy Nhung, YAMAGUCHI Masanori, YOSHIDA Tadashi, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    共著, Springer, 2010年, 英語

    学術書

  • 微生物増殖学の現在・未来

    吉田 健一

    共著, 地人書館, 2008年01月, 日本語

    教科書・概説・概論

講演・口頭発表等

  • 有用希少イノシトールを生産する枯草菌細胞工場

    吉田 健一

    日本農芸化学会2019年度大会, 2019年, 日本語, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 有用希少イノシトールを生産する枯草菌細胞工場

    吉田 健一

    第13回日本ゲノム微生物学会年会, 2019年, 日本語, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 枯草菌におけるルシフェラーゼ発光によるNADPH再生のモニタリング

    吉田 健一

    第13回日本ゲノム微生物学会年会, 2019年, 日本語, 国内会議

    ポスター発表

  • myo-Inositol-1-phosphate synthase “restored” in Bacillus subtilis to produce scyllo-inositol, a therapeutic agent for Alzheimer's disease, from glucose

    吉田 健一

    10th Conference on Recombinant Protein Production, 2019年, 英語, 国際会議

    口頭発表(一般)

  • Monitoring NADPH regeneration by luciferase luminescence in Bacillus subtilis

    吉田 健一

    BACELL2019, 2019年, 英語, 国際会議

    口頭発表(一般)

  • Engineering conjugation in Gram-positive bacteria

    吉田 健一

    The 10th International Symposium of Innovative BioProduction Kobe, 2019年, 英語, 国際会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • Bacillus velezensis S141 facilitates the development of nodules in soybean with Bradyrhizobium diazoefficiens USDA110

    吉田 健一

    5th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation, 2019年, 英語, 国際会議

    ポスター発表

  • ARMC5変異を認めたPBMAHと異所性副甲状腺腫によるPHPTの合併例

    苛原 彩, 福岡 秀規, 吉田 健一, 土橋 一生, 坂東 弘教, 岡田 裕子, 井口 元三, 小川 裕行, 神澤 真紀, 坂井 誠, 臼井 健, 小川 渉, 高橋 裕

    臨床内分泌代謝Update, 2018年11月, 日本語, 福岡, 国内会議

    ポスター発表

  • Luscan-Lumish症候群による巨人症発症機序の解析

    隅田 健太郎, 福岡 秀規, 井口 元三, 蟹江 慶太郎, 藤田 泰功, 小武 由紀子, 吉田 健一, 坂東 弘教, 高橋 路子, 千原 和夫, 鳴海 覚志, 長谷川 奉延, 小川 渉, 高橋 裕

    第91回日本内分泌学会学術総会, 2018年04月, 日本語, 宮崎, 国内会議

    口頭発表(一般)

  • ACTH下垂体腺腫におけるErbB4受容体の役割の解明

    小武 由紀子, 福岡 秀規, 吉田 健一, 山田 正三, 井口 元三, 小川渉, 高橋裕

    日本内分泌学会学術総会, 2018年04月, 日本語, 宮崎, 国内会議

    ポスター発表

  • 枯草菌における人工的NADPH再生供給系の駆動とその効果

    吉田 健一

    2018年度度グラム陽性菌ゲノム機能会議, 2018年, 日本語, 国内会議

    口頭発表(一般)

  • ダイズ根粒菌のPHB蓄積制御を司るPhaRの多面的遺伝子発現調節

    吉田 健一

    日本ゲノム微生物学会2017年度大会, 2018年, 日本語, 国内会議

    口頭発表(一般)

  • ダイズ-ダイズ根粒菌の共生窒素固定を促進するBacillus velezensis S141のゲノム解析

    吉田 健一

    2018年度度グラム陽性菌ゲノム機能会議, 2018年, 日本語, 国内会議

    口頭発表(一般)

  • Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for selftransfer

    吉田 健一

    BACELL2018, 2018年, 英語, 国際会議

    口頭発表(一般)

  • Production of scyllo-inositol: conversion of rice bran into a promising disease-modifying therapeutic agent for Alzheimer's disease

    吉田 健一

    The Third International Symposium on Rice Science in Global Health, 2018年, 英語, 国際会議

    ポスター発表

  • Geobacillus kaustophilusの新規形質転換法の開発

    吉田 健一

    2018年度度グラム陽性菌ゲノム機能会議, 2018年, 日本語, 国内会議

    ポスター発表

  • Enzyme secretion in Bacillus subtilis enhanced by customization of signal peptides

    吉田 健一

    9th International Symposium of Innovative BioProduction Kobe (iBioK), 2018年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Controlling Redox State of NADP+

    吉田 健一

    Metabolic engineering 12, 2018年, 英語, 国際会議

    ポスター発表

  • Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation

    吉田 健一

    13th European Nitrogen Fixation Conference, 2018年, 英語, 国際会議

    口頭発表(一般)

  • Bacillus subtilis engineered for production of scyllo-inositol from glucose

    吉田 健一

    ASBA2018, 2018年, 英語, 国際会議

    ポスター発表

  • Bacillus subtilis cell factory converting agricultural wastes into rare inositol

    吉田 健一

    Second Interdisciplinary and Research Alumni Symposium iJaDe2018, 2018年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Bacillus Subtilis Cell Factories for Production of Two Inositol-Stereoisomers from Glucose

    吉田 健一

    Metabolic engineering 12, 2018年, 英語, 国際会議

    口頭発表(一般)

  • Bacillus subtilis cell factories converting agricultural wastes into scyllo-inositol

    吉田 健一

    2018 iBioN, The Symposium on Biorefinery and Biprocess Topics, 2018 - Innovative Bio-production of Fuels and Chemicals, 2018年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 100 kbを超える枯草菌染色体DNAセグメントの簡便迅速な接合伝達

    吉田 健一

    第70回日本生物工学会大会, 2018年, 日本語, 国内会議

    口頭発表(一般)

  • 農業廃棄物から作る有用希少イノシトール

    吉田 健一

    Visionary 農芸化学100 シンポジウム, 2017年, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 接合伝達を利用した Leuconostoc mesenteroides の形質転換

    吉田 健一

    2017年年度度グラム陽性菌ゲノム機能会議, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • 枯草菌を宿主としたL-gluconate生産系の開発

    吉田 健一

    日本農芸化学会2017年度大会, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • ダイズ根粒菌の共生窒素固定を促進する PGPR (Bacillus velezensis S141) のゲノム解析

    吉田 健一

    2017年年度度グラム陽性菌ゲノム機能会議, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • コリネ型細菌を用いたフェルラ酸からプロトカテク酸の生産

    吉田 健一

    日本農芸化学会2017年度大会, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • Recombinant enzyme secretion in Bacillus subtilis enhanced by customization of signal peptides

    吉田 健一

    National University of Singapore, 2017年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Recombinant enzyme secretion in Bacillus subtilis enhanced by customization of signal peptides

    吉田 健一

    Institut für Systembiotechnologie, Universität des Saarlandes, 2017年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Recombinant enzyme secretion in Bacillus subtilis enhanced by customization of signal peptides

    吉田 健一

    9th Conference on Recombinant Protein Production, 2017年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Production of rare inositols: conversion of agricultural wastes into value added products

    吉田 健一

    IUMS2017, Singapore, 2017年, 英語, 国際会議

    口頭発表(一般)

  • Production of rare inositols: conversion of agricultural wastes into value added products

    吉田 健一

    The 8th International Symposium of Innovative BioProduction Kobe, 2017年, 英語, 国際会議

    口頭発表(一般)

  • Production of myo-inositol from glucose in Bacillus subtilis

    吉田 健一

    2017年年度度グラム陽性菌ゲノム機能会議, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • Lactococcus lactis subsp. cremoris FC 株のポリサッカライド合成遺伝子群の同定

    吉田 健一

    第11回ゲノム微生物学会, 2017年, 日本語, 国内会議

    ポスター発表

  • Geobacillus kaustophilus の pLS20 系プラスミド形質転換とその増殖フェーズ依存性

    吉田 健一

    2017年年度度グラム陽性菌ゲノム機能会議, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • Geobacillus kaustophilus Crh is independent of glucose catabolite repression but represses inositol catabolic genes

    吉田 健一

    19th International Conference on Bacilli & Gram-Positive Bacteria, 2017年, 英語, 国際会議

    口頭発表(一般)

  • Chryseobacterium sp. 5-3B由来N-アセチルトランスフェラーゼの基質認識部位の探索

    吉田 健一

    日本農芸化学会2017年度大会, 2017年, 日本語, 国内会議

    口頭発表(一般)

  • Bio-production in Kobe and a new generation of genome editing

    吉田 健一

    Rector’s Lecture, Uniwersytet Mikołaja Kopernika, 2017年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Bacillus subtilis cell factory converting phytic acid into scyllo-inositol, a therapeutic agent for Alzheimer's disease

    吉田 健一

    Enzyme Engineering XXIV, 2017年, 英語, 国際会議

    口頭発表(一般)

  • Bacillus subtilis cell factory converting agricultural wastes into rare inositol isomer

    吉田 健一

    Micalis, INRA Jouy-en-Josas, 2017年, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 納豆菌由来接合伝達プラスミドを用いたGeobacillus kaustophilusの形質転換

    吉田 健一

    2016年年度度グラム陽性菌ゲノム機能会議, 2016年, 日本語, 国内会議

    口頭発表(一般)

  • 納豆菌由来pLS20cat を用いた新規プラスミドベクターシステムの開発

    吉田 健一

    第68回 日本生物工学会大会, 2016年, 日本語, 国内会議

    ポスター発表

  • 枯草菌のiolH がイノシトール代謝において果たす生理的意義

    吉田 健一

    第68回 日本生物工学会大会, 2016年, 日本語, 国内会議

    ポスター発表

  • 枯草菌によるミニセルロソームの構築

    吉田 健一

    2016年年度度グラム陽性菌ゲノム機能会議, 2016年, 日本語, 国内会議

    ポスター発表

  • ハイマンノース型糖鎖を有するAspergillus glaucus MA0196 由来アスパルティックプロテアーゼの特性解析

    吉田 健一

    第68回 日本生物工学会大会, 2016年, 日本語, 国内会議

    ポスター発表

  • ダイズ由来の新規健康増進化合物ピニトールの開発

    吉田 健一

    平成28年度 神戸大学大学院農学研究科 公開講座, 2016年, 日本語, 国内会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • ダイズ植物由来機能性成分ピニトール:その特徴と期待される健康効果

    吉田 健一

    日本応用糖質科学会近畿支部 第42回支部会, 2016年, 日本語, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • ダイズ根粒菌におけるPHB蓄積制御を司る転写因子PhaRのゲノムワイドな遺伝子発現制御

    吉田 健一

    日本農芸化学会関西支部例会(第497回講演会), 2016年, 日本語, 国内会議

    口頭発表(一般)

  • Possible common regulators of ytsJ for malic enzyme and gndA for 6-phosphogluconate dehydrogenase in Bacillus subtilis

    吉田 健一

    BACELL2016, 2016年, 英語, 国際会議

    口頭発表(一般)

  • Inositol catabolism in Geobacillus kaustophilus is repressed by Crh phosphorylated independently of glucose catabolite repression

    吉田 健一

    2nd International Conference on Post-Translational Modifications in Bacteria, 2016年, 英語, 国際会議

    口頭発表(一般)

  • Inactivation of PhaR involved in poly-beta-hydroxybutyrate accumulation in Bradyrhizobium japonicum USDA110 and its pleiotropic effects

    吉田 健一

    12th European Nitrogen Fixation Conference, 2016年, 英語, 国際会議

    口頭発表(一般)

  • Further improvement of Bacillus subtilis cell factory producing scyllo-inositol, a promising therapeutic agent for Alzheimer’s disease

    吉田 健一

    Metabolic Engineering 11, 2016年, 英語, 国際会議

    口頭発表(一般)

  • degU32変異による枯草菌のカタボライト抑制解除の機構

    吉田 健一

    2016年年度度グラム陽性菌ゲノム機能会議, 2016年, 日本語, 国内会議

    口頭発表(一般)

  • ダイズ根粒菌のphaR欠損変異は多面的な表現型変化を引き起こす

    吉田 健一

    日本農芸化学会関西支部例会(第492回講演会), 2015年12月, 日本語, 国内会議

    口頭発表(一般)

  • 細菌由来ジアミントランスアミナーゼ遺伝子のクローニングと発現酵素の特性解析

    吉田 健一

    第67回日本生物工学会大会(2015), 2015年10月, 日本語, 国内会議

    ポスター発表

  • かつお節のかび付けに使用されるAspergillus 属糸状菌由来アスパルティックプロテアーゼの特性解析

    吉田 健一

    第67回日本生物工学会大会(2015), 2015年10月, 日本語, 国内会議

    ポスター発表

  • Chryseobacterium sp. 5-3B 株由来N-アセチルトランスフェラーゼの部位特異的アミノ酸置換と機能解析

    吉田 健一

    第67回日本生物工学会大会(2015), 2015年10月, 日本語, 国内会議

    ポスター発表

  • Bradyrhizobium japonicum のPHB 蓄積に関わるファジン遺伝子の解析

    吉田 健一

    第67回日本生物工学会大会(2015), 2015年10月, 日本語, 国内会議

    ポスター発表

  • 枯草菌を用いたL-glucose 生産系の開発

    吉田 健一

    2015 年度グラム陽性菌ゲノム機能会議, 2015年08月, 日本語, 国内会議

    口頭発表(一般)

  • 枯草菌Rok のytsJ およびgndA プロモーターへの結合

    吉田 健一

    2015 年度グラム陽性菌ゲノム機能会議, 2015年08月, 日本語, 国内会議

    口頭発表(一般)

  • Geobacillus kaustophilus HTA426 のIolE が転写制御に関与する可能性

    吉田 健一

    2015 年度グラム陽性菌ゲノム機能会議, 2015年08月, 日本語, 国内会議

    口頭発表(一般)

  • Enhanced production of bio-based chemicals by using Bacillus subtilis genome reduced strain as a platform cell factory

    吉田 健一

    8th International Conference on Gram-Positive Microorganisms, 2015年06月, 英語, 国際会議

    ポスター発表

  • A third generation of Bacillus subtilis cell factory for producing scyllo-inositol

    吉田 健一

    8th International Conference on Gram-Positive Microorganisms, 2015年06月, 英語, 国際会議

    ポスター発表

  • A Hyperphosphorylation of DegU interferes CcpA-dependent catabolite repression of rocG in Bacillus subtilis

    吉田 健一

    8th International Conference on Gram-Positive Microorganisms, 2015年06月, 英語, 国際会議

    口頭発表(一般)

  • Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis

    吉田 健一

    BACELL2015, 2015年04月, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Bacillus subtilis cell factory engineered for efficient bioconversion of myo-inositol into scyllo-inisitol, a therapeutic agent for Alzheimer's disease

    吉田 健一

    International Conference on Metabolic Engineering in Bacteria, 2015年04月, 英語, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 枯草菌DegUの過剰なリン酸化昂進はカタボライト抑制を早期に解除する

    吉田 健一

    第9回日本ゲノム微生物学会年会, 2015年03月, 日本語, 国内会議

    口頭発表(一般)

  • Geobacillus kaustphillusのイノシトール代謝系遺伝子群の発現制御

    吉田 健一

    2015年度日本農芸化学会大会, 2015年03月, 日本語, 国内会議

    口頭発表(一般)

  • Chryseobacterium sp. 5-3B由来N-アセチルトランスフェラーゼの発現と特性解析

    吉田 健一

    2015年度日本農芸化学会大会, 2015年03月, 日本語, 国内会議

    口頭発表(一般)

  • アルファルファ根粒菌に見出されたイノシトール合成系に関する研究

    吉田 健一

    日本農芸化学会関西支部例会(第487回講演会), 2014年12月, 日本語, 国内会議

    口頭発表(一般)

  • 枯草菌フィターゼ分泌の高度効率化

    吉田 健一

    第66回 日本生物工学会大会, 2014年09月, 日本語, 国内会議

    ポスター発表

  • 枯草菌フィターゼ分泌の高度効率化

    吉田 健一

    2014年度グラム陽性菌ゲノム機能会議, 2014年09月, 日本語, 国内会議

    口頭発表(一般)

  • ハイマンノース型糖鎖を有する Aspergillus glaucus MA0196 由来アスパルティックプロテアーゼの特性解析

    吉田 健一

    第66回 日本生物工学会大会, 2014年09月, 日本語, 国内会議

    ポスター発表

  • グルコン酸代謝システムを用いた、標的遺伝子の一時的な誘導(パルス=インダクションシステム)の構築

    吉田 健一

    2014年度グラム陽性菌ゲノム機能会議, 2014年09月, 日本語, 国内会議

    ポスター発表

  • イノシトール資化性微生物の検索と分離菌におけるiol遺伝子群の解析

    吉田 健一

    2014年度グラム陽性菌ゲノム機能会議, 2014年09月, 日本語, 国内会議

    ポスター発表

  • To be, or not to be: that is the question. - myo-inositol in Sinorhizobium meliloti

    吉田 健一

    The 11th European Nitrogen Fixation Conference, 2014年09月, 英語, Tenerife, Spain, 国際会議

    ポスター発表

  • Geobacillus kaustophilus HTA426のiol遺伝子群の発現調節

    吉田 健一

    2014年度グラム陽性菌ゲノム機能会議, 2014年09月, 日本語, 国内会議

    口頭発表(一般)

  • Chryseobacterium sp. 5-3B 由来 N - アセチルトランスフェラーゼの特製解析

    吉田 健一

    第66回 日本生物工学会大会, 2014年09月, 日本語, 国内会議

    ポスター発表

  • A Bacillus subtilis cell factory efficiently converting myo-inositol into scyllo-inositol, a potential therapeutic agent for Alzheimer's disease

    吉田 健一

    IUMS2014, 2014年07月, 英語, Montreal, Canada, 国際会議

    シンポジウム・ワークショップパネル(公募)

  • Bacterial cell factory for production of scyllo-inositol, a potential therapeutic agent for Alzheimer’s disease

    吉田 健一

    Metabolic Engineering X, 2014年06月, 英語, Cancouver, Canada, 国際会議

    口頭発表(一般)

  • Inositol dehydrogenases in Geobacillus kaustophilus are regulated by a Crh homolog but not by glucose

    吉田 健一

    BACELL2014, 2014年04月, 英語, Bratislava, Slovakia, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 枯草菌を用いたシロ-イノシトールの高効率バイオコンバージョン法の確立

    吉田 健一, 田中 耕生

    第8回日本ゲノム微生物学会年会, 2014年03月, 日本語, 国内会議

    口頭発表(一般)

  • アルツハイマー病への適応が期待されるシロ-イノシトールを生産する枯草菌細胞工場

    吉田 健一

    2014年度日本農芸化学会大会, 2014年03月, 日本語, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • Bacillus subtilisFP-133由来耐塩性アミラーゼの特性解析

    吉田 健一

    2014年度日本農芸化学会大会, 2014年03月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • Possible inositol synthesis in rhizobia with physiological significance?

    吉田 健一

    One day workshop in "Lessons from interaction between plant and microbe: strategies in symbiosis and competition", 2013年11月, 英語, Kobe University Brussels European Centre, 国際会議

    シンポジウム・ワークショップパネル(指名)

  • Takenaka. Poly-β-hydroxybutyrate (PHB) accumulation in Bradyrhizobium japonicum depends on proteins referred to as phasins

    吉田 健一

    BioMicroWorld 2013, 2013年10月, 英語, Universidad Complutense de Madrid (Complutense University of Madrid), 国際会議

    口頭発表(一般)

  • Physiological significance of possible myo-inositol synthesis in Sinorhizobium meliloti

    吉田 健一

    18th International Congress on Nitrogen Fixation, 2013年10月, 英語, Phoenix Seagaia Resort, Miyazaki, Japan, 国際会議

    口頭発表(一般)

  • A Bacillus subtilis cell factory to produce syllo-inositol, a disease-modifying therapeutic agent for Alzheimer's disease

    吉田 健一

    BioMicroWorld 2013, 2013年10月, 英語, Universidad Complutense de Madrid (Complutense University of Madrid), 国際会議

    口頭発表(一般)

  • 枯草菌フィターゼ分泌の高度効率化

    吉田 健一

    第65回日本生物工学会大会, 2013年09月, 日本語, 広島国際会議場, 国内会議

    ポスター発表

  • 枯草菌フィターゼ分泌の高度効率化

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 日本語, 筑波山ホテル江戸屋, 国内会議

    ポスター発表

  • 枯草菌のNADPH再生機構.2013年度グラム陽性菌ゲノム機能会議

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 日本語, 筑波山ホテル江戸屋, 国内会議

    口頭発表(一般)

  • 枯草菌によるシローイノシトール生産の効率化

    吉田 健一

    第65回日本生物工学会大会, 2013年09月, 日本語, 広島国際会議場, 国内会議

    ポスター発表

  • 枯草菌アセトイン/2,3-ブタンジオール発酵の効率化

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 日本語, 筑波山ホテル江戸屋, 国内会議

    ポスター発表

  • シローイノシトールの高効率バイオコンバージョン法の確立

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 日本語, 筑波山ホテル江戸屋, 国内会議

    口頭発表(一般)

  • かつお節のかび付けに使用されるAspergillus glaucus MA0196由来アスパルティックプロテアーゼの特性解析

    吉田 健一

    第65回日本生物工学会大会, 2013年09月, 日本語, 広島国際会議場, 国内会議

    ポスター発表

  • イノシトール-1-リン酸合成酵素とイノシトールモノフォスファターゼの融合

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 日本語, 筑波山ホテル江戸屋, 国内会議

    ポスター発表

  • Rational strategies to switch cofactor specificity of inositol dehydrogenases of Bacillus subtilis

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 英語, 筑波山ホテル江戸屋, 国内会議

    ポスター発表

  • Pseudomonas aeruginosa ME-4由来エステラーゼを用いた卵殻膜の可溶化と生理活性ペプチドの探索

    吉田 健一

    日本農芸化学会関西・中四国・西日本支部 日本ビタミン学会近畿・中国四国・九州沖縄地区 2013年度 合同広島大会, 2013年09月, 日本語, 広島県立大学, 国内会議

    口頭発表(一般)

  • Optimizing secretion of heterologous thermostable cellulases in Bacillus subtilis

    吉田 健一

    日本農芸化学会関西・中四国・西日本支部 日本ビタミン学会近畿・中国四国・九州沖縄地区 2013年度 合同広島大会, 2013年09月, 英語, 広島県立大学, 国内会議

    口頭発表(一般)

  • Chryseobacterium sp. 5-3由来N-アセチルトランスフェラーゼの発現と特性解析

    吉田 健一

    第65回日本生物工学会大会, 2013年09月, 日本語, 広島国際会議場, 国内会議

    ポスター発表

  • Bacillus subtilis FP-133由来C末端領域欠損アミラーゼの特性解析

    吉田 健一

    2013年度グラム陽性菌ゲノム機能会議, 2013年09月, 日本語, 筑波山ホテル江戸屋, 国内会議

    ポスター発表

  • Identification, characterization, and comparative analysis of tannnase from Lactobacillus plantarum, L. paraplantarum, and L. pentosus

    吉田 健一

    7th International Conference on Gram-positive Microorganisms, 2013年06月, 英語, 国際会議

    ポスター発表

  • Efficient bioconversion of myo-inositol to scyllo-inositol by genetically modified Bacillus suntilis requires global metabolic change to improve NADPH regeneration

    吉田 健一

    7th International Conference on Gram-positive Microorganisms, 2013年06月, 英語, Montecatini Terme, Tuscany, Italy, 国際会議

    ポスター発表

  • Characterization of a helotolerant extracellular amylase from Bacillus subtilis FP-133

    吉田 健一

    7th International Conference on Gram-positive Microorganisms, 2013年06月, 英語, Montecatini Terme, Tuscany, Italy, 国際会議

    ポスター発表

  • Bacillus subtilis iolH encodes an additional triosephosphate isomerase

    吉田 健一

    7th International Conference on Gram-positive Microorganisms, 2013年06月, 英語, Montecatini Terme, Tuscany, Italy, 国際会議

    ポスター発表

  • Bacillus subtilis possesses two distinct triosephosphate isomerases

    吉田 健一

    BACELL 2013, 2013年04月, 英語, Newcastle University, UK, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • アルファルファ根粒菌のイノシトール合成系はストレスで誘導される

    吉田 健一

    日本農芸化学会2013年度大会, 2013年03月, 日本語, 東北大学, 国内会議

    口頭発表(一般)

  • Chryseobacterium sp. 5-3由来N-アセチルトランスフェラーゼの遺伝子クローニングと発現

    吉田 健一

    日本農芸化学会2013年度大会, 2013年03月, 日本語, 東北大学, 国内会議

    口頭発表(一般)

  • Bradyrhizobium japonicumのPHB蓄積に関わるパラログ遺伝子の機能解析

    吉田 健一

    第7回日本ゲノム微生物学会年会, 2013年03月, 日本語, 長浜バイオ大学, 国内会議

    口頭発表(一般)

  • 枯草菌を活用する生理活性イノシトールの開発

    吉田 健一

    JBA発酵と代謝研究会講演会美味しい健康生活は微生物が作る, 2013年02月, 日本語, 京都大学, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 芳香族炭化水素受容体依存的な多剤耐性遺伝子Mdr1の転写調節機構

    三谷 塁一, 木根原 匡希, 吉田 健一, 芦田 均

    動物細胞工学会JAACT2013, 2013年, 日本語, 福井, 国内会議

    ポスター発表

  • Pseudomonas aeruginosa ME-4由来エラスターゼを用いた卵殻膜の可溶化と生理活性ペプチドの探索

    竹中 慎治, 田中 裕基, 芦田 均, 吉田 健一

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会, 2013年, 日本語, 広島, 国内会議

    口頭発表(一般)

  • Aryl hydrocarbon receptor enhances the expression of multidrug-registant Mdr1b through p53 in mouse hepatoma cells

    MITANI Takakazu, KINEHARA Masaki, YOSHIDA Ken-ichi, ASHIDA Hitoshi Ashida

    The 33rd International Symposium on Halogenated Persistent Organic Pollutants and POPs (DIOXIN2013), 2013年, 英語, Daegu, Korea, 国際会議

    ポスター発表

  • Bio-production of biologically active substances: achievements and perspectives of iBioK

    吉田 健一

    The 4th International Symposium of Innovative Bioproduction Kobe (iBioK), 2013年01月, 英語, Kobe University, 国際会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • Possible myo-inositol synthesis in Sinorhizobium meliloti

    吉田 健一

    The 2nd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation, 2012年10月, 英語, Hilton Phuket Arcadia Resort & Spa, Thailand, 国際会議

    ポスター発表

  • Bacillus subtilis cell factory for production of scyllo-inositol promising for Alzheimer’s disease

    吉田 健一

    The 90th Anniversary Meeting, The Society for Biotechnology, Japan, 2012年10月, 英語, 神戸国際会議場, 国際会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • アルファルファ根粒菌におけるイノシトール合成系の存在と意義

    吉田 健一

    植物微生物研究会第22回研究交流会, 2012年09月, 日本語, 神戸大学, 国内会議

    口頭発表(一般)

  • PhaP phasins of Bradyrhizobium japonicum playing an important role in poly--hydroxybutyrate synthesis

    吉田 健一

    10th European Nitrogen Fixation Conference, 2012年09月, 英語, Munich University, 国際会議

    ポスター発表

  • PhaP phasins of Bradyrhizobium japonicum playing an important role in poly-beta-hydroxybutyrate synthesis

    吉田 健一

    10th European Nitrogen Fixation Conference, 2012年09月, 英語, Munich University, 国際会議

    ポスター発表

  • 乳酸菌由来タンナーゼの配列多様性と酵素活性に関する研究

    吉田 健一

    平成24年度グラム陽性菌のゲノム生物学研究会, 2012年08月, 日本語, 焼津グランドホテル, 国内会議

    ポスター発表

  • 新規イノシトール合成酵素の特性解析と応用

    吉田 健一

    24年度グラム陽性菌のゲノム生物学研究会, 2012年08月, 日本語, 焼津グランドホテル, 国内会議

    ポスター発表

  • 枯草菌におけるNADPH再生バランス機構の理解とその応用平成

    吉田 健一

    24年度グラム陽性菌のゲノム生物学研究会, 2012年08月, 日本語, 焼津グランドホテル, 国内会議

    ポスター発表

  • 枯草菌iolHの生理学的及び酵素学的機能の解明

    吉田 健一

    24年度グラム陽性菌のゲノム生物学研究会, 2012年08月, 日本語, 焼津グランドホテル, 国内会議

    ポスター発表

  • Geobacillus kaustophilus HTA426が有する3種イノシトール脱水素酵素の生理的意義

    吉田 健一

    平成24年度グラム陽性菌のゲノム生物学研究会, 2012年08月, 日本語, 焼津グランドホテル, 国内会議

    口頭発表(一般)

  • Bacillus subtilis FP-133由来耐塩性アミラーゼの特性解析

    吉田 健一

    24年度グラム陽性菌のゲノム生物学研究会, 2012年08月, 日本語, 焼津グランドホテル, 国内会議

    ポスター発表

  • A cell factory of Bacillus subtilis engineered for the simple bioconversion of myo-inositol to scyllo-inositol, a potential therapeutic agent for Alzheimer's disease

    吉田 健一

    Metabolic Engineering IX: Metabolic Engineering and Synthetic Biology, 2012年06月, 英語, Biarritz, France, 国際会議

    ポスター発表

  • Bacillus subtilis cell factory producing scyllo-inositol, a potential therapeutic agent for Alzheimer’s disease

    吉田 健一

    BACELL 2012, 2012年04月, 英語, Trinity College, Dublin, Ireland, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 人為的なイノシトール異性体バイオコンバージョンが誘発するトランスクリプトーム変動

    吉田 健一

    第6回日本ゲノム微生物学会年会, 2012年03月, 日本語, 立教大学, 国内会議

    口頭発表(一般)

  • バクテリア細胞内でのイノシトール立体異性体相互変換の可能性

    吉田 健一

    日本農芸化学会2012年度(平成24年度)大会, 2012年03月, 日本語, 京都女子大学, 国内会議

    口頭発表(一般)

  • Chryseobacterium so. 5-3B由来N-アセチルトランスフェラーゼの特性解析

    吉田 健一

    日本農芸化学会2012年度(平成24年度)大会, 2012年03月, 日本語, 京都女子大学, 国内会議

    口頭発表(一般)

  • 4-アミノピリジンの分解に関わる微生物群集の組成と分解経路の推定

    吉田 健一

    日本農芸化学会2012年度(平成24年度)大会, 2012年03月, 日本語, 京都女子大学, 国内会議

    口頭発表(一般)

  • 根粒菌に見出されたミオ-イノシトール-1-リン酸合成酵素

    吉田 健一

    2011年度日本農芸化学会関西支部第472回講演会, 2011年12月, 日本語, 神戸大学, 国内会議

    口頭発表(一般)

  • 枯草菌の代謝改変による有用希少イノシトールの生産

    吉田 健一

    日本生物工学会 第1回代謝工学研究部会シンポジウム, 2011年11月, 日本語, 大阪大学, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 枯草菌によるイノ シトール異性体変換の効率化

    吉田 健一

    2011年度日本農化会関西・中部支部合同大会, 2011年10月, 日本語, 京都大学, 国内会議

    口頭発表(一般)

  • かつお節のかび付けに使用されるAspergillus glaucus MA0196由来アスパルティックプロテアーゼの精製と特性解析

    吉田 健一

    2011年度日本農化会関西・中部支部合同大会, 2011年10月, 日本語, 京都大学, 国内会議

    口頭発表(一般)

  • 納豆菌を使った健康増進成分の生産について

    吉田 健一

    灘酒研究会講演会, 2011年09月, 日本語, 灘酒研究会, 国内会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • かつお節のかび付けに用いられるAspergillus repens MK82 由来アスパルティックプロテアーゼII の精製と特性解析

    吉田 健一

    第63回日本生物工学会大会, 2011年09月, 日本語, 東京農業工業大学, 国内会議

    口頭発表(一般)

  • Sinorhizobium melilotiのミオ-イノシトール-1-リン酸合成酵素

    吉田 健一

    植微微生物研究会 研究交流会, 2011年09月, 日本語, 岡山大学, 国内会議

    ポスター発表

  • Geobacillus kaustophilus HTA426 の3種のイノシトール脱水素酵素パラログ

    吉田 健一

    日本ゲノム微生物学会「ゲノム微生物学会ワークショップ -ゲノムで繋がる微生物研究の新展開-」, 2011年09月, 日本語, 国内会議

    ポスター発表

  • Geobacillus kaustophilus HTA426 のイノシトール脱水素酵素遺伝子の解析

    吉田 健一

    第63回日本生物工学会大会, 2011年09月, 日本語, 東京農業工業大学, 国内会議

    口頭発表(一般)

  • Gene cloning and expression of an eggshell membrane decomposing protease from Pseudomonas aeruginosa strain ME-4

    吉田 健一

    IUMS2011, 2011年09月, 英語, Sapporo COnvention Center, 国際会議

    ポスター発表

  • Bacillus subtilis cell factory for production of rare inositols

    吉田 健一

    IUMS2011, 2011年09月, 日本語, Sapporo Convention Center, 国際会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 枯草菌によるイノシトール異性体変換の精密測定と効率化条件の検討

    吉田 健一

    平成23年度グラム陽性菌ゲノム機能会議, 2011年08月, 日本語, 福山大学, 国内会議

    口頭発表(一般)

  • 枯草菌iolH遺伝子の酵素学的及び生理学的機能の解明

    吉田 健一

    平成23年度グラム陽性菌ゲノム機能会議, 2011年08月, 日本語, 福山大学, 国内会議

    ポスター発表

  • 枯草菌degU32変異によるカタボライト抑制の積極的解除

    吉田 健一

    平成23年度グラム陽性菌ゲノム機能会議, 2011年08月, 日本語, 福山大学, 国内会議

    口頭発表(一般)

  • Geobacillus kaustophilus HTA426の3種iolGパラログが同時発現する意義の考察

    吉田 健一

    平成23年度グラム陽性菌ゲノム機能会議, 2011年08月, 日本語, 福山大学, 国内会議

    ポスター発表

  • Bacillus subtilis FP-133由来耐塩性酵素の特性解析

    吉田 健一

    平成23年度グラム陽性菌ゲノム機能会議, 2011年08月, 日本語, 福山大学, 国内会議

    口頭発表(一般)

  • Three functional inositol dehydrogenases of Geobacillus kaustophilus HTA426 encoded by a single operon

    吉田 健一

    6th International Conference on Gram-positive Microorganisms, 2011年06月, 英語, Montecatini Terme, Tuscany, Italy, 国際会議

    ポスター発表

  • Characterization of halotolerant extracellular enzymes from Bacillus subtilis FP-133

    吉田 健一

    6th International Conference on Gram-positive Microorganisms, 2011年06月, 英語, Montecatini Terme, Tuscany, Italy, 国際会議

    ポスター発表

  • Bacillus subtilis cell factory for bio-production

    吉田 健一

    6th International Conference on Gram-positive Microorganisms, 2011年06月, 英語, Montecatini Terme, Tuscany, Italy, 国際会議

    ポスター発表

  • かつお節のかび付けに使用されるAspergillus glaucus MA0196由来アスパルティックプロテアーゼの精製と特性解析

    吉田 健一

    日本農芸化学会大会, 2011年03月, 日本語, 京都大学, 国内会議

    口頭発表(一般)

  • 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産

    吉田 健一, 蓮沼 誠久

    第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議

    口頭発表(一般)

  • マウス肝腫瘍由来Hepa-1c1c7細胞におけるアリール炭化水素受容体 (AhR) を介した多剤耐性遺伝子mdr1bの発現誘導

    北野 嶺, 木根原 匡希, 福田 伊津子, 吉田 健一, 芦田 均

    第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010), 2010年, 日本語, 国内会議

    ポスター発表

  • パスウエイデザイン枯草菌による2種の有用希少イノシトールの選択生産

    森永 哲郎, 芦田 均, 吉田 健一

    日本農芸化学会2010年度大会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • 納豆発酵におけるγ-PGA生産に重要な役割を果たす納豆菌菌体外プロテアーゼの解析

    加田 茂樹, 大岩 好, 石川 篤志, 加賀 孝之, 芦田 均, 吉田 健一

    日本農芸化学会2009年度大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 納豆発酵におけるγ-PGA 生産に重要な役割を果たす菌体外プロテアーゼの解析

    加田 茂樹, 伊藤 治美, 大岩 好, 石川 篤志, 加賀 孝之, 芦田 均, 吉田 健一

    2009年度グラム陽性細菌ゲノム機能会議, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 枯草菌転写因子DegUによるグルタミン酸脱水素酵素遺伝子rocGの転写制御

    松瀬 貴嗣, 森永 哲郎, 芦田 均, 吉田 健一

    第32回日本分子生物学会年会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 枯草菌をモデルとした新規抗菌薬剤の作用メカニズム解析

    吉田 健一, 熊田 祐士, 芦田 均

    第3回日本ゲノム微生物学会年会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 枯草菌のscyllo-inositol脱水素酵素遺伝子とその転写調節因子の同定

    森永 哲郎, 芦田 均, 吉田 健一

    第32回日本分子生物学会年会, 2009年, 日本語, 国内会議

    ポスター発表

  • 筋肉組織における糖輸送担体GLUT4の細胞膜移行に及ぼすプロポリス抽出物の影響について

    上田 学, 澤田 圭介, 柏田 大輔, 福田 伊津子, 吉田 健一, 芦田 均

    日本食品学工学会第56回大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • 筋肉細胞におけるアシル化カテキンによるGLUT4膜移行促進効果とその作用機構について

    上田 学, 布施 直也, 水品 善之, 吉田 弘美, 福田 伊津子, 吉田 健一, 芦田 均

    第14回日本フードファクター学会, 2009年, 日本語, 国内会議

    ポスター発表

  • ヨモギ抽出物によるGLUT4膜移行促進作用機構の解明

    上田 学, 川崎 健吾, 山本 憲朗, 室崎 伸二, 福田 伊津子, 吉田 健一, 芦田 均

    日本農芸化学会2009年度大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • ダイズ由来ピニトールの血糖値降下作用と肥満抑制

    吉田 健一, Dang Thuy Nhung, 三本木 あずさ, 吉田 正, 芦田 均

    日本農芸化学開関西支部第462回講演会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • scyllo-Inositol metabolism in Bacillus subtilis

    MORINAGA Tetsuro, ASHIDA Hitoshi, YOSHIDA Ken-ichi

    BACELL2009, 2009年, 英語, 国際会議

    口頭発表(一般)

  • L6筋管細胞におけるアシルカテキンによるインスリン応答性糖輸送担体(GLUT4)の細胞膜移行促進効果

    布施 直也, 上田 学, 福田 伊津子, 吉田 健一, 水品 善之, 吉田 弘美, 芦田 均

    日本農芸化学会2009年度大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • Geobacillus kaustophilusのイノシトール資化不全変異株取得

    三本木 あずさ, 松瀬 貴嗣, 森永 哲郎, 鈴木 宏和, 芦田 均, 吉田 健一

    日本農芸化学会2009年度大会, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • Geobacillus kaustophilusの3 種のイノシトール脱水素酵素をコードするオペロン

    三本木 あずさ, 松瀬 貴嗣, 森永 哲郎, 鈴木 宏和, 芦田 均, 吉田 健一

    2009年度グラム陽性細菌ゲノム機能会議, 2009年, 日本語, 国内会議

    口頭発表(一般)

  • Geobacillus kaustophilus HTA426の3種のイノシトール脱水素酵素をコードするオペロン

    三本木 あずさ, 松瀬 貴嗣, 森永 哲郎, 鈴木 宏和, 芦田 均, 吉田 健一

    第32回日本分子生物学会年会, 2009年, 日本語, 国内会議

    ポスター発表

  • Epigallocatechin-3-gallate regulate glucose metabolism in skeletal muscle cells

    UEDA Manabu, KAWABATA Kyuichi, FUKUDA Itsuko, YOSHIDA Ken-ichi, ASHIDA Hitoshi

    The 4th International Conference on Polyphenols and Health, 2009年, 英語, 国際会議

    ポスター発表

  • A bioconversion process to produce scyllo-inositol, a promising drug candidate for Alzheimer's disease

    MORINAGA Tetsuro, ASHIDA Hitoshi, YOSHIDA Ken-ichi

    5th International Conference on Gram-positive Microorganisms, 2009年, 英語, 国際会議

    ポスター発表

  • 2’,3’,4’-Trihydroxy-2- phenylacetophenone derivatives, a novel and potent class of anti-Gram-positive antibacterial agents

    YOSHIDA Ken-ichi, KUMADA Yuji, ASHIDA Hitoshi

    5th International Conference on Gram-positive Microorganisms, 2009年, 英語, 国際会議

    口頭発表(一般)

  • Functional analysis of NodD transcription factor paralogs of Sinorhizobium fredii USDA191 involved in regulation of the nodulation genes

    Ken-ichi Yoshida

    7th European Nitrogen Fixation Conference, 2006年07月, 英語, 7th European Nitrogen Fixation Conference, Aarhus Denmark, 国際会議

    ポスター発表

  • Prevention of dioxin toxicity by food factores

    ASHIDA Hitoshi, NISHIUMI Shin, MUKAI Rie, YOSHIDA Kenichi, FUKUDA Itsuko

    2005 International chemical congress of pacific basin societies (PACIFICHEM 2005) December 15th-20th, Programp.4TECH, #162, Abstract is available on CD., 2005年12月, 英語, 未記入, Honolulu, Hawaii, 国際会議

    口頭発表(一般)

  • Functional analysis of NodD transcription factor paralogs of Shinorhizobium fredii USDA191 involved in regulation of the nodulation genes.

    YOSHIDA Kenichi, KINEHARA M, IKEUTI M, KURIMOTO Emi, KIM W.-S, KRISHNAN H.B, ASHIDA Hitoshi

    Rikkyo International Symposium ' From bacteria to organelle', 2005年08月, 英語, 立教大学理学部, 東京, 国内会議

    口頭発表(一般)

  • 茶の飲用はアリール炭化水素受容体の活性化を抑制する

    福田 伊津子, 西海 信, 坂根 巌, 藪下 善行, 沢村 信一, 金沢 和樹, 吉田 健一, 芦田 均

    2005年度日本農芸化学会大会講演要旨集, 2005年03月, 日本語, 日本農芸化学会, 札幌, 国内会議

    口頭発表(一般)

  • 紅茶の飲用がラットのインスリン感受性組織の脂質代謝に及ぼす影響

    久保 麻友子, 吉田 健一, 芦田 均

    日本農芸化学会第438回講演会 講演要旨集p.7, 2005年, 日本語, 日本農芸化学会, 京都, 国内会議

    口頭発表(一般)

  • 枯草草の薬剤耐性に関与する転写因子のレギュロン機能解析

    松岡 浩史, 広岡 和丈, 吉田 健一, 藤田 泰太郎

    日本農芸化学会2005年度大会 大会講演要旨集p.65, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議

    口頭発表(一般)

  • 枯草菌イノシトール分解系を応用したD-chiro-inositol の発酵生産

    吉田 健一, 山口 将憲, 芦田 均, 藤田 泰太郎

    日本農芸化学会2005年度大会 大会講演要旨集p.224, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議

    口頭発表(一般)

  • 枯草菌イノシトール分解系に関与するiolG とiolI の新規機能

    森永 哲郎, 山口 将憲, 池内 摩耶, 木根原 匡希, 芦田 均, 藤田 泰太郎, 吉田 健一

    日本農芸化学会2005年度関西・中四国・西日本支部合同大会 講演要旨集p.82, 2005年, 日本語, 日本農芸化学会, 大阪, 国内会議

    口頭発表(一般)

  • 枯草菌HTH蛋白質の機能解析-脂肪酸分解に関わるHTH転写制御因子の解析

    松岡 浩史, 吉田 健一, 広岡 和丈, 藤田 泰太郎

    第28回日本分子生物学会年会 講演要旨集p.415, 2005年, 日本語, 日本分子生物学会, 博多, 国内会議

    ポスター発表

  • モロヘイヤはアリール炭化水素受容体の形質転換を抑制する

    西海 信, 福田 伊津子, 向井 理恵, 吉田 健一, 芦田 均

    日本動物細胞工学会2005年度大会 講演要旨集p.58, 2005年, 日本語, 日本動物細胞工学会, 東京, 国内会議

    口頭発表(一般)

  • フラボノイド類とアリール炭化水素受容体との相互作用について

    向井 理恵, 福田 伊津子, 西海 信, 川瀬 雅也, 吉田 健一, 芦田 均

    日本農芸化学会2005年度関西・中四国・西日本支部合同大会 講演要旨集p.75, 2005年, 日本語, 日本農芸化学会, 大阪, 国内会議

    口頭発表(一般)

  • ダイオキシン受容体AhRの大腸菌内での発現精製とその機能解析

    木根原 匡希, 吉田 健一, 芦田 均

    第28回日本分子生物学会年会 講演要旨集p.439, 2005年, 日本語, 日本分子生物学会, 博多, 国内会議

    ポスター発表

  • クルクミンのダイオキシン毒性抑制効果について

    西海 信, 吉田 健一, 芦田 均

    第20回香辛料研究会 講演要旨集p.25, 2005年, 日本語, 日本香辛料研究会, 京都, 国内会議

    口頭発表(一般)

  • カテキンがインスリン応答性糖輸送活性に及ぼす影響

    青木 由葵子, 福田 伊津子, 吉田 健一, 芦田 均

    日本農芸化学会2005年度大会 大会講演要旨集p.285, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議

    口頭発表(一般)

  • インジゴイドがアリール炭化水素受容体の形質転換に及ぼす影響について

    西海 信, 山本 憲朗, 小土井 理恵, 福田 伊津子, 室崎 伸二, 吉田 健一, 芦田 均

    第10回日本フードファクター学会(JSoFF) 講演要旨集p.61, 2005年, 日本語, 日本フードファクター学会, 岡山, 国内会議

    口頭発表(一般)

  • イノシトール分解系を応用したピニトール強化納豆の作製

    森永 哲郎, 山口 将憲, 吉田 健一, 芦田 均

    第10回日本フードファクター学会(JSoFF) 講演要旨集p.53, 2005年, 日本語, 日本フードファクター学会, 岡山, 国内会議

    口頭発表(一般)

  • アントラキノン類が示す新規生理活性:グリコース輸送担体の機能変調

    白杉 一郎, 青木 由葵子, 吉田 健一, 芦田 均

    日本農芸化学会第438回講演会 講演要旨集p.8, 2005年, 日本語, 日本農芸化学会, 京都, 国内会議

    口頭発表(一般)

  • アリール炭化水素受容体複合体に対するフラボノイド類の作用機序の解明

    向井 理恵, 福田 伊津子, 西海 信, 川瀬 雅也, 吉田 健一, 芦田 均

    第28回日本分子生物学会年会 講演要旨集p.479, 2005年, 日本語, 日本分子生物学会, 博多, 国内会議

    ポスター発表

  • アリール炭化水素受容体の形質転換に影響をおよぼす植物の検索

    西海 信, 細川 敬三, 菱田 敦之, 向井 理恵, 福田 伊津子, 吉田 健一, 芦田 均

    日本農芸化学会2005年度大会 大会講演要旨集p.118, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議

    口頭発表(一般)

  • Transcription of Bacillus subtilis asnH operon under the dual control of AbrB abd CodY is stabilized by the 5'-untranslated region of its transcript containing a long sequence triplication

    YOSHIDA Kenichi, IGARASHI K, MORINAGA T, KOBAYASHI K, ASHIDA Hitoshi, FUJITA Y

    13th International Conference in Bacilli, Abstract book T79, 2005年, 英語, 未記入, San Diego, California, 国際会議

    口頭発表(一般)

  • Sinorhizobium fredii USDA191のnodD1/nodD2パラログの機能解析

    木根原 匡希, 向井 理恵, 池内 摩耶, 栗本 恵美, 芦田 均, 吉田 健一

    第15回植物微生物研究会, 2005年, 日本語, 植物微生物研究会, 高松, 国内会議

    口頭発表(一般)

  • Sinorhizobium fredii USDA191 NodD1 の大腸菌内での発現精製

    池内 摩耶, 木根原 匡希, 栗本 恵美, 芦田 均, 吉田 健一

    第15回植物微生物研究会, 2005年, 日本語, 植物微生物研究会, 高松, 国内会議

    口頭発表(一般)

  • (-)-エピガロカテキンガレートとアリール炭化水素受容体との相互作用について

    向井 理恵, 福田 伊津子, 西海 信, 川瀬 雅也, 吉田 健一, 芦田 均

    日本農芸化学会2005年度大会 大会講演要旨集p.99, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議

    口頭発表(一般)

  • 納豆菌(枯草菌)のイノシトール分解系の全貌解明

    吉田 健一

    第1回機能性食品開発研究会, 2004年11月, 日本語, 未記入, 大阪商工会議所, 国内会議

    口頭発表(一般)

  • 神戸大学農学部生物機能化学科生物機能開発化学教育研究分野

    芦田 均, 吉田 健一, 福田 伊津子, 木根原 匡希, 久保 麻友子, 西海 信, 青木 由葵子, 向井 理恵

    近畿地域アグリビジネス創出フェア, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 枯草菌窒素代謝制御因子TnrAによるilv-leuオペロンの制御

    東條 繁郎, 松岡 浩史, 森崎 薫, 里村 武範, 吉田 健一, 藤田 泰太郎

    2004年度日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • 枯草菌機能未知HTH制御因子のレギュロン解析

    吉田 健一

    第2回情報生命学研究交流会, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 枯草菌の薬剤耐性に関与する転写制御因子の探索とそのレギュロンの解析

    松岡 浩史, 吉田 健一, 藤田 泰太郎

    グラム陽性菌のゲノム生物学研究会T02, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 枯草菌の薬剤耐性に関与する可能性のある転写制御因子の制御ターゲットの探索

    松岡 浩史, 多木 陽平, 吉田 健一, 藤田 泰太郎

    2004年度日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • 枯草菌のグルタミン依存性アスパラギン合成酵素パラログの発現制御と機能の解析

    森永 哲郎, 吉田 健一

    岡山・島根・鳥取大学交流会, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 枯草菌のグルタミン依存性アスパラギン合成酵素パラログの発現制御と機能の解析

    吉田 健一, 森永 哲郎, 佐藤 勉, 高松 宏, 五十嵐 光地, 藤田 泰太郎

    グラム陽性菌のゲノム生物学研究会T21, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 枯草菌のグルタミン依存性アスパラギン合成酵素パラログの機能と発現制御の解析

    吉田 健一, 森永 哲郎, 芦田 均

    日本農芸化学会第437回講演会, 2004年, 日本語, 日本農芸化学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 枯草菌のクエン酸サイクルに関与する遺伝子発現制御因子のマイクロアレイ解析

    里村 武範, 東條 繁郎, 吉田 健一, 藤田 泰太郎

    2004年度日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • 枯草菌のイノシトール分解系の全貌解明とその応用

    吉田 健一

    はりま産学交流会・拡大一日神戸大学神戸大学の知的資産の競演、あなたが選ぶ!!シーズコンペ!!, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 枯草菌のイノシトール分解系の逆遺伝学~遺伝子機能の同定と転写制御機構の解明~

    吉田 健一, 藤田 泰太郎

    2004年度日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • 枯草菌asnHオペロンの発現調節

    吉田 健一, 小林 和夫, 藤田 泰太郎

    2004年度日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • 筋肉細胞のグルコース取り込み活性に及ぼすアントラキノン類の影響

    白杉 一郎, 青木 由葵子, 別所 宏昭, 吉田 健一, 芦田 均

    第9回日本フードファクター学会, 2004年, 日本語, 日本フードファクター学会, 淡路夢舞台国際会議場, 国内会議

    口頭発表(一般)

  • 逆遺伝学的アプローチによる枯草菌イノシトール分解系の解明

    吉田 健一

    奈良先端大Bio-COEセミナー, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • モデル微生物としての枯草菌~ポストゲノム時代の逆遺伝学研究~

    吉田 健一

    ミニ国際シンポジウム:マリンゲノムの新展開「深海微生物のゲノム生物学」, 2004年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • Tea has the potential to reduce the dioxin risk.

    FUKUDA Itsuko, SAKANE I, NISHIUMI Shin, SHIRASUGI I, SAWAMURA S, KANAZAWA Kazuki, YOSHIDA Kenichi, ASHIDA Hitoshi

    International Conference of O-CHA(tea) Culture and Science., 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Tea has the potential to reduce the dioxin risk.

    FUKUDA Itsuko, SAKANE I, NISHIUMI Shin, SHIRASUGI Ichirou, SAWAMURA S, KANAZAWA Kazuki, YOSHIDA Kenichi, ASHIDA Hitoshi

    2004 International Conference On O-CHA(tea) Culture And Science, 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Suppressive effects of catechins on differentiation of 3T3-L1 preadipocytes.

    AOKI Y, HASHIMOTO Takashi, YOSHIDA Kenichi, ASHIDA Hitoshi

    International Conference of O-CHA(tea) Culture and Science., 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Suppressive effects of catechins on differentiation of 3T3-L1 preadipocyte.

    AOKI Y, HASHIMOTO Takashi, YOSHIDA Kenichi, ASHIDA Hitoshi

    2004 International Conference On O-CHA(tea) Culture And Science, 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Reverse genetics of myo-inositol catabolism in bacteria.

    吉田 健一

    奈良女子大学理学部外来セミナー, 2004年, 英語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • Identification of a 2-keto-myo-inositol dehydratase gene of Shinorhizobium fredii USDA191

    YOSHIDA Kenichi, Kim W-S, TANAKA Y, ASHIDA Hitoshi, FUJITA Y, Krishnan H B

    第27回日本分子生物学会, 2004年, 日本語, 日本分子生物学会, 神戸, 国内会議

    口頭発表(一般)

  • Black tea (Cameria sinensis) suppresses hyperglycemia in STZ-induced diabetic rats.

    KUBO M, SAKANE I, SAWAMURA S, YOSHIDA Kenichi, ASHIDA Hitoshi

    International Conference of O-CHA(tea) Culture and Science., 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

共同研究・競争的資金等の研究課題

産業財産権

  • アセトイン産生細胞および当該細胞を用いたアセトインの製造方法

    吉田 健一

    特願2010-256714, 2010年11月17日, 大学長, 特許5737650, 2015年05月01日

    特許権

  • シロ-イノシトール産生細胞および当該細胞を用いたシロ-イノシトール製造方法(米)

    吉田 健一, 芦田 均

    13/127047, 2009年10月30日, 大学長, 8962287, 2015年02月24日

    特許権

  • 好熱性微生物の形質転換方法

    鈴木 宏和, 吉田 健一

    特願2010-083543, 2010年03月31日, 大学長, 特許5673996, 2015年01月09日

    特許権

  • シロ-イノシトール産生細胞および当該細胞を用いたシロ-イノシトール製造方法

    吉田 健一, 芦田 均

    特願2010-535683, 2009年10月30日, 大学長, 特許5649119, 2014年11月21日

    特許権

  • シロ-イノシトール産生細胞および当該細胞を用いたシロ-イノシトール製造方法(仏)

    吉田 健一, 芦田 均

    09823344.8, 2009年10月30日, 大学長, 2357222, 2014年08月13日

    特許権

  • シロ-イノシトール産生細胞および当該細胞を用いたシロ-イノシトール製造方法(独)

    吉田 健一, 芦田 均

    09823344.8, 2009年10月30日, 大学長, 602009026044.8, 2014年08月13日

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