片山 寛則 | ![]() |
カタヤマ ヒロノリ | |
大学院農学研究科 附属食資源教育研究センター | |
准教授 | |
農学関係 |
2015年11月 日本学術振興会, 科研費審査員表彰
科学研究費助成事業に有意義かつ公平な審査意見を付していただいた審査委員を選考し表彰する, 日本国その他の賞
2012年10月 神戸大学, 学長表彰特別賞, 神戸大学東日本大震災復興支援事業’校庭にイワテヤマナシの花を咲かせよう’
片山寛則氏は、東日本大震災に見舞われた被災地の復興を願い、平成23年度からイワテヤマナシの苗木を、復興のシンボルとして被災地の学校に贈る神戸大学復興支援プロジェクト「校庭にイワテヤマナシの花を咲かせよう」を展開している。昨年12月、片山准教授を中心とした食資源センターの教職員が津波で被災した三陸沿岸南部地域の陸前高田市から宮古市までの小中高等学校、特別支援学校43校を訪問し結果、約半数の学校から苗木を育ててみたいという返答を得、今年3月には片山准教授に加え、その他農学研究科関係者も参加して吉里小学校、高浜小学校、宮古水産高校にて、生徒たちとイワテヤマナシの祈念植樹を行った。さらに、宮古水産高校ではイワテヤマナシを材料として加工品を開発し、地元の活性化を目指す取り組みにつながっている。これら一連の活動は朝日新聞夕刊の1面トップ記事として大きく取り上げら, 日本国その他の賞
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Two new species of Camellia (Theaceae) are described from Vietnam: Camellia vuquangensis Luong, Tran et L. T. Nguyen and Camellia hatinhensis Luong, Tran et L. T. Nguyen. The new taxa were col- lected from Vu Quang National Park, Central of Vietnam. The new finds are morphologically dissimilar to all known Camellia species. Recent Camellia discoveries have increased the number
Korean Society of Plant Taxonomists, 2018年06月, Korean Journal of Plant Taxonomy, 48, 115 - 122, 英語[査読有り]
研究論文(学術雑誌)
Camellia tuyenquangensis (ツバキ科)はベトナムのTuyen Quang県、Chiem Hoa村で見つかった黄色の新種である。Camellia tuyenquangensisはCamellia luongiiと類似しているが狭卵形の葉、無毛の小包葉、毛のある萼片、割けた柱頭等の形態的な差異が見られた。形態的データはCamellia sect. Chrysantha Changに位置すべきと考えられた。
Korean Society of Plant Taxonomists, 2017年08月, Korean Journal of Plant Taxonomy, 47 (2), 95 - 99, 英語[査読有り]
研究論文(学術雑誌)
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研究論文(学術雑誌)
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研究論文(学術雑誌)
Although ornamental pear trees (Pyrus calleryana) are popular in North America and Australia, we have no ornamental pear cultivar in Japan. Two pear accessions available for ornamental trees were selected from 'Iwateyamanashi' (Pyrus ussuriensis var. aromatica) endemic in northern Japan. 95 accessions deposited as germplasm collection at Kobe University were selected first with the PCA based on the 9 floral morphological traits such as numbers of petal, stamen, stigma, flowers per bud, pedicel length, flower diameter, colour of petal edge, petal length and petal width, and flowering date, tree form, fruits quality and autumnal leaf colouration. And then 30 accessions were chosen from evenly considering PCA score plot. Finally two accessions of 'i1717' and 'i1525' were organoleptically evaluated by 123 panelists for full size pictures of flower, corymb and flowering branch. They had longer petal length, flower diameter, and petal width compared to those of other accessions and the petal shapes were circle. By PCA and the organoleptic evaluation, 'i1525' and 'i1759' were selected. The former showed rose pink petal edge, better fruit quality, and compact tree form. The latter possessed better fruit quality and compact tree shape, and the leaf turned a deep red colour in the fall. Two accessions had unique ornamental traits not found in Japanese pear cultivars (Pyrus pyrifolia). Pear genetic resources collected from the northern part of Japan will provide new resources for breeding and industry.
International Society for Horticultural Science, 2015年09月15日, Acta Horticulturae, 1094, 117 - 122, 英語[査読有り]
研究論文(国際会議プロシーディングス)
Iwateyamanashi' (Pyrus ussuriensis var. aromatica) is one of the Pyrus species grown wild in Japan. They have been utilized traditionally as food and medicine, but are not used now. Therefore, it has been decreasing, so conservation and evaluation are urgently needed. Over 750 accessions including Iwateyamanashi were collected and maintained at Kobe University. Antioxidant capacity of accessions from the collection maintained to evaluate its functional characters were analysed. In 2010 and 2011, we measured total polyphenol content and DPPH radical scavenging activity at mature stage among 29 accessions. In 2013, we evaluated temporal total polyphenol content and DPPH radical scavenging activity among 3 accessions. Almost 'Iwateyamanashi' had much higher antioxidant capacity than Japanese pear cultivars (P. pyrifolia) and European pear cultivars (P. communis L.). Especially, accession 'i0868' contained much polyphenol (1.40 mg g-1 FW of (-)-epicatechin equivalent) and showed high DPPH radical scavenging activity (19.76 μmol g-1 FW of ascorbic acid equivalent). It indicated about 23 times higher polyphenol content and about 33 times higher DPPH radical scavenging activity than 'Hosui'. The fruit used in this study was found to possess high antioxidant capacity in an early development stage, and then it reduced according to increasing of fruit weight at full maturity. 'Iwateyamanashi' with high antioxidant capacity might stimulate the new demands as breeding materials and health foods.
International Society for Horticultural Science, 2015年09月15日, Acta Horticulturae, 1094, 539 - 548, 英語[査読有り]
研究論文(国際会議プロシーディングス)
Pyrus ussriensis Maxim. is native to the northern part of China, but whose habitats are currently being destroyed by environmental changes and human deforestation. An investigation of population structure and genetic diversity of wild Ussurian pear is a priority in order to acquire fundamental knowledge for conservation. A total of 153 individuals of wild Ussurian pear from t
2015年08月, PLoS ONE, 10 (8), 1 - 20, 英語[査読有り]
研究論文(学術雑誌)
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研究論文(学術雑誌)
Since chloroplasts are maternally inherited and have unique features in evolution, their genome sequences have been broadly used in phylogenetic studies of plants. Here, we assembled the chloroplast genome sequence of cultivated pineapple (Ananas comosus (L.) Merr.) that is the most economically significant plant in the Bromeliaceae using next-generation sequencers. The genome
Springer, 2015年05月, Tree Genetics & Genomes, 11 (60), 英語[査読有り]
研究論文(学術雑誌)
浸漬液および浸漬時間の違いが日本短角種牛肉の理化学特性に及ぼす影響について検討を行った.4 頭の日本短角種去勢牛の大腿二頭筋をパイナップル果汁(pH3.5),豊水果汁(pH4.4),イワテヤマナシ果汁(pH3.9),および RO水(pH5.9)に 0,24,48,および 96 時間浸漬させた.また,別の 4 頭の日本短角種去勢牛の大腿二頭筋を pH4.01 標準緩衝液,pH6.86 標準緩衝液,および RO 水(pH5.8)に 0 および 24 時間浸漬させた.浸漬後,ドリップロス,クッキングロス,色調値,および最大荷重を測定した.クッキングロスは,イワテヤマナシ果汁が RO 水およびパイナップル果汁に比較して浸漬 48 時間まで有意に低かった.最大荷重は,パイナップル果汁が他の浸漬液に比較して浸漬 96 時間で有意に低くなったが,イワテヤマナシ果汁
日本畜産学会, 2015年02月, 日本畜産学会報, 86 (1), 37 - 43, 日本語[査読有り]
研究論文(学術雑誌)
枝変わり突然変異解明のモデルシステムとして八重咲き源平咲ハナモモから花弁のアントシアニン色素の発現を制御するMyb様転写因子を単離し‘PEACE’と名づけた。Peaceのハナモモ花弁での一過的発現システムを構築し白地花弁でアンントシアニン色素を発現させた。トランスポゾンは見つからなかった。斑入りの原因はPEACE遺伝子を制御する上流遺伝子の変異またはエピジェネティック変異である可能性が示唆された。
Oxford Journal, 2014年03月, Journal of Experimental Botany, 65 (4), 1081 - 1094, 英語[査読有り]
研究論文(学術雑誌)
Some local cultivars and wilds of Iwateyamanashi (Pyrus ussuriensis var. aromatica) that grows wild in Northern Tohoku, Japan have good aromatic fruit. Iwateyamanashi may be valuable germplasms as a donor of odor compounds in breeding of Japanese pear (Pyrus pyrifolia), because almost all Japanese pear cultivars have faint odor. Fruits odors from a local cultivar 'Sanenashi', a wild accession (i0830) in Iwateyamanashi, cultivars of 'Kosui' and 'La France' were characterized at first with comparative Aroma Extract Dilution Analysis (AEDA). Application of AEDA, based on Gas chromatography/Olfactometry analysis (GC/O), on the odor concentration prepared from 'Sanenashi' indicated the presence of 33 odor-active compounds including methyl and ethyl esters, aldehydes and alcohol. The eleven odor compounds from 16 accessions of Iwateyamanashi showed various combinations and wide range of odor concentrations by Principal Component Analysis (PCA). Especially 2 accessions of local cultivar 'Natsunashi' plotted in the highly ethyl ester group might be useful for Japanese pear breeding.
JAPANESE SOC BREEDING, 2013年03月, BREEDING SCIENCE, 63 (1), 86 - 95, 英語[査読有り]
研究論文(学術雑誌)
In order to understand the genetic diversity of wild Ussurian pears in China, chloroplast DNA (cpDNA) of 186 wild accessions from 12 populations in Inner Mongolia, Heilongjiang and Jilin Provinces and 51 Chinese and European pear cultivars including Pyrus ussuriensis, Pyrus pyrifolia, Pyrus bretschneideri, Pyrus sinkiangensis and Pyruscommunis were investigated. Each accession was classified into one of three types (types A, B and C) based on two large deletions in the hypervariable regions between the accD-psaI and rps16-trnQ genes. Thirty haplotypes were identified by 32 mutations including 17 gaps (in/dels) and 15 base changes. Haplotype network analysis revealed that wild Chinese Ussurian pears could be grouped into subgroup I of type A. A haplotype, Hcp3, in subgroup I detected in Heilongjiang and Jilin Provinces was considered to be a divergent centre in Chinese Ussurian pears. However, the genetic diversity of wild accessions revealed by the two hypervariable regions was quite low. In particular, 98 % of wild Ussurian accessions in Inner Mongolia shared an identical haplotype Hcp1 and are, therefore, monomorphic. In comparison, Chinese pear cultivars were more divergent. These results suggest that the cpDNAs from wild Ussurian pears in Inner Mongolia have specifically differentiated compared to those from pears of other areas. The number of wild Ussurian pears has been decreasing because of desertification and land development, therefore conservation is needed. © 2012 Springer-Verlag.
Springer-Verlag, 2013年, Tree Genetics and Genomes, 9 (1), 167 - 177, 英語[査読有り]
研究論文(学術雑誌)
By use of Bayesian statistical inference and allelic data for 18 microsatellite loci, we analyzed the genetic structure of Chinese, Korean, and Japanese pear cultivars and of native populations of Pyrus ussuriensis. Although Japanese pear cultivars had a simple genetic structure, Chinese and Korean pear cultivars were admixures of Japanese pear and native P. ussuriensis from the Asian continent. Genetic differentiation between groups of native populations and those of cultivars was high, but cultivars were not well differentiated from each other. Chinese and Korean cultivars, which have traditionally been classified as either P. ussuriensis, P. bretschneideri, or P. pyrifolia, were much closer to Japanese cultivars, which have traditionally been classified as P. pyrifolia, than to native P. ussuriensis. We propose a new classification of cultivars by using the Group concept in accordance with the International Nomenclature for Cultivated Plants, namely, the Pyrus Ussurian pear group, the Pyrus Chinese white pear group, the Pyrus Chinese sand pear group, and the Pyrus Japanese pear group.
SPRINGER WIEN, 2012年11月, PLANT SYSTEMATICS AND EVOLUTION, 298 (9), 1689 - 1700, 英語[査読有り]
研究論文(学術雑誌)
The chloroplast genome of Pyrus was found to be 159,922 bp in length which included a pair of inverted repeats (IRs) of 26,392 bp, separated by a small single-copy region of 19,237 bp and a large single-copy region (LSC) of 87,901 bp. A total of 130 predicted genes (113 unique genes and 17 genes, which were duplicated in the IR) including 79 protein-coding genes, four ribosomal RNA genes and 30 tRNA genes were identified based on similarity to homologs from the chloroplast genome of Nicotiana tabacum. Genome organization was very similar to the inferred ancestral angiosperm chloroplast genome. Comparisons between Pyrus, Malus, and Prunus in Rosaceae revealed 220 indels (a parts per thousand yen10 bp). Excluding ycf1 and ycf2, which contained deletions in the coding region, all of these were detected in the spacer or intron regions. Three insertions and 13 deletions were detected in Pyrus compared to the same loci in Malus and Prunus. After comparing 89 noncoding chloroplast DNA regions in Pyrus and Malus, highly variable regions such as ndhC-trnV and trnR-atpA were identified. In Pyrus and Malus, the IR/LSC borders were 62 bp shorter than those of Prunus. In addition, there were length mutations at the IRa/LSC junction and in trnH. A total of 67 simple sequence repeats (more than 10 repeated motifs) were identified in the Pyrus chloroplast genome. The indels and simple sequence repeats will be useful evolutionary tools at both intra- and interspecific levels. Phylogenetic analysis demonstrated a close relationship between Pyrus and Prunus in the Rosaceae.
SPRINGER HEIDELBERG, 2012年08月, TREE GENETICS & GENOMES, 8 (4), 841 - 854, 英語[査読有り]
研究論文(学術雑誌)
The genome structure of pear chloroplast DNA (cpDNA) is extremely highly conserved in comparison with that of other angiosperms, and therefore, relatively few phylogenetic analyses for pear (Pyrus spp.) have been carried out using cpDNA as a marker. In this study, we identified two hypervariable regions in intergenic spacers of cpDNA from 21 species in Pyrus. One of these regions is 857 bp in length and lies between the accD-psaI genes, and the other is a 904-bp region between the rps16-trnQ genes. The mutation rate of gaps for the two regions was 10 and 26 times higher, respectively, than the base change rate. Twenty-five haplotypes were revealed among 21 species in Pyrus by 36 mutations found in the two regions. These included 27 gaps and 9 base changes but excluded cpSSRs. Phylogenetic relationships between the 25 haplotypes were generated by haplotype network analysis. The 25 haplotypes represented three groups (types A-C) with two large deletions, one 228 bp in length between the accD-psaI genes and the other 141 bp between the rps16-trnQ genes. Types A and B consisted mostly of pears native to East and South Asia. Type C contained mainly Pyrus communis and wild relatives native to Europe, West and Central Asia, Russia, and Africa. Type B might have diverged from primitives such as pea pears in type A. Phylogenetic utility of structural alterations (gaps) occurring in the hypervariable regions of Pyrus cpDNA is discussed.
SPRINGER HEIDELBERG, 2012年04月, TREE GENETICS & GENOMES, 8 (2), 313 - 326, 英語[査読有り]
研究論文(学術雑誌)
The mechanism underpinning flower colour variegation in single petal flowering peach (Prunus persica) was investigated. This tree bears three different types of flowers within one tree, i.e., deep pink coloured flowers (P1), flowers with pink sectors on a pale pink background (PPS1), and flowers with pink sectors on a white background (W1). In spite of the difference in flower colour appearance, all three petal types possess same pigment of cyanidin-3-gulcoside as the major pigment. The differences in petal colour could be attributed to differences in pigment content. Structural genes of the anthocyanin biosynthetic pathway showed no differences in structure at the level of homologous Southern hybridization. But expression of the genes encoding the biosynthetic enzymes CHI, F3H, DFR and ANS was lower in PPS1 and W1 petals compared to P1 petals.
INT SOC HORTICULTURAL SCIENCE, 2012年, XXVIII INTERNATIONAL HORTICULTURAL CONGRESS ON SCIENCE AND HORTICULTURE FOR PEOPLE (IHC2010): INTERNATIONAL SYMPOSIUM ON GENOMICS AND GENETIC TRANSFORMATION OF HORTICULTURAL CROPS, 929, 287 - 292, 英語[査読有り]
研究論文(国際会議プロシーディングス)
The region of distribution of Pyrus L., endemic in northern Japan including 'Iwateyamanashi', (Pyrus ussuriensis var. aromatic author) has been decreasing, thus conservation and evaluation are urgently needed. 750 pear accessions were collected and deposited at Kobe University in an ex situ field genebank. The 30 landraces in this collection were established by top grafting to evaluate morphological and agronomical fruit traits of. Flowering date, harvest date, fruit shape, skin color, diameter, height, peduncle length, fresh weight, number of locules, calyx persistency, number of sepals, flavor, firmness, sugar content (Brix), pH, and astringency were investigated for two years. Sugar content ranged from 9 to 16.1%. pH of fruit extract ranged from 3.0 to 4.8. Fruit having a high acid content are suitable for cooking or processing. Days from full bloom to harvest ranged from 94 to 209. By sensory evaluation, eleven accessions were revealed to have sweet and strong flavors previously undetected in modern Japanese pear cultivars. Early maturing cultivars had a significant tendency to have a strong flavor. One landrace, 'Sanenashi' was seedless. Until the 1940s, 'Sanenashi' fruit was used for canning. These threatened landraces possessed unique agronomic traits not found in named Japanese pear cultivars. Pear genetic resources collected from northern Japan will provide new resources for breeding and industry.
INT SOC HORTICULTURAL SCIENCE, 2011年, XXVIII INTERNATIONAL HORTICULTURAL CONGRESS ON SCIENCE AND HORTICULTURE FOR PEOPLE (IHC2010): III INTERNATIONAL SYMPOSIUM ON PLANT GENETIC RESOURCES, 918, 971 - 982, 英語[査読有り]
研究論文(国際会議プロシーディングス)
Hybridization between native and cultivated species is a concern in conservation biology. However, detecting such hybridization and distinguishing true natives from prehistorically naturalized species based on phenotypic characteristics is difficult. Here, we report on introgression between native and prehistorically introduced pear (Pyrus) species in Northern Tohoku (northern end of Honshu Island), Japan. We analyzed 20 microsatellites in 226 wild, seemingly wild, or cultivated materials. Phenetic analysis showed that wild Japanese populations of P. ussuriensis var. ussuriensis in Northern Tohoku, previously considered true natives based on morphology and phytogeography, differed from those in continental Asia, confirming their nativeness. However, Bayesian inference of population structures showed that Japanese P. ussuriensis was genetically admixed with two genetic clusters: true native P. ussuriensis var. ussuriensis and prehistorically introduced P. pyrifolia. Even in the Kitakami Mountains, where true native populations of var. ussuriensis are believed to persist, most wild trees were at least somewhat admixed. Prehistorically introduced then naturalized plants are treated as natives in Japan's conservation management, and some are considered endangered. However, introgression of prehistorically naturalized P. pyrifolia into threatened native P. ussuriensis var. ussuriensis has occurred. This paper examines the implications for conservation management.
SPRINGER, 2010年02月, CONSERVATION GENETICS, 11 (1), 115 - 126, 英語[査読有り]
研究論文(学術雑誌)
Iwateyamanashi (Pyrus ussuriensis var. aromatica) is one of the Pyrus species which grows wild in Japan. The number of Iwateyamanashi trees has been decreasing, so conservation and evaluation is urgently needed. Over 500 accessions of Pyrus species collected from Iwate in northern Tohoku region are maintained at Kobe University as an Iwateyamanashi germplasm collection. In order to investigate the genetic diversity, five SSR (simple sequence repeat) markers, developed from Japanese and European pear were examined for 86 Pyrus individuals including 58 accessions from Iwate. These SSR loci could discriminate between all the Iwate accessions except for 10 that bear seedless fruit, as well as determine the genetic diversity in Iwateyamanashi germplasms. High levels of variation were detected in 41 alleles and the mean observed heterozygosity across 5 loci was 0.50 for the Iwate accessions. Seedless accessions sharing identical SSR genotype with the local pear variety "Iwatetanenashi" were supposed to have been propagated vegetatively via grafting. In an UPGMA phenogram, Japanese pear varieties (P. pyrifolia) were clustered into two groups with some Iwate accessions including seedless ones. Another 38 Iwate accessions were not clustered clearly, and there was no clear relationship between these accessions and geographical distribution or morphological characters. Allele frequency revealed that the Iwate accessions were genetically more divergent than the Japanese pear varieties. Most Japanese pears possessed a 219 bp deletion at a spacer region between the accD and psaI genes in the chloroplast DNA (cpDNA), but other Pyrus species and two Iwateyamanashi trees did not. In the Iwate accessions, 79.3% had a deletion type cpDNA and others had a standard type cpDNA without deletion. These results are indicative of the wide range of genetic diversity in the Iwate accessions which include Japanese pear varieties. A combination of SSR and cpDNA analyses revealed high heterogeneity in Iwateyamanashi and coexistence of Iwateyamanashi and hybrid progeny with P. pyrifolia. These could be reasons for the wide range of continuous morphological variation described previously.
SPRINGER, 2007年11月, GENETIC RESOURCES AND CROP EVOLUTION, 54 (7), 1573 - 1585, 英語[査読有り]
研究論文(学術雑誌)
東北地方に自生するとされるイワテヤマナシの保全、利用を目的としてイワテヤマナシの探索、聞き取り調査、樹高、幹周、果実の形態計測を行った。青森県、岩手県、秋田県の全市町村を網羅する探索により現存数(615個体)、分布域、30種類の地方名、過去の利用法、6種類の果実形質から高い多様性などが判明し、遺伝資源としての有用性を報告した。このうち250本を神戸大学で生息域外保存した。
Springer-Verlag, 2006年04月, Genetic Resources and Crop Evolution, 53, 483 - 498, 英語[査読有り]
研究論文(学術雑誌)
研究論文(学術雑誌)
In order to understand the evolutionary aspects
of the chloroplast DNA (cpDNA) structures in Rosaceous plants, a physical map of peach (Prunus persica cv. Hakuhou) cpDNA was constructed. Fourteen lambda phage clones which covered the entire sequence of the peach cpDNA were digested by restriction enzymes (SalI, XhoI, BamHI, SacI, and PstI) used singly or in combination. The mol
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研究論文(学術雑誌)
A physical map of chloroplast DNA (cpDNA) of pear [Pyrus ussuriensis var. hondoensis (Nakai et Kikuchi) Rehder] was constructed using five restriction enzymes, SalI, XhoI, BamHI, SacI and PstI. This information will make it possible to investigate the phylogenetic relationships between Pyrus species. Pear cpDNA was found to be a circular molecule with a total size of about 156 kb in which two inverted repeats of 24.8 kb divide the molecule into small (17 kb) and large (90 kb) single-copy regions. The endonuclease recognition sites in the physical map were determined by single and double digestion of 13 lambda phage clones which covered the entire sequence of the pear cpDNA. Twenty nine genes were localized on the physical map of the pear cpDNA. The structure of pear cpDNA was almost the same in terms of genome size and gene order as that of tobacco cpDNA. RFLP analysis was carried out on cpDNAs from five Pyrus species (Pyrus pyrifolia, Pyrus ussuriensis, Pyrus calleryana, Pyrus elaeagrifolia and Pyrus communis). Two mutations, a recognition-site mutation and a length mutation (deletion), were found only in the cpDNA of P pyrifolia cultivars. These mutations were localized on the physical map of pear cpDNA. The number of mutations of cpDNA in Pyrus species are small in comparison with those of other angiosperms, suggesting a high degree of genome conservatism in Pyrus species.
SPRINGER-VERLAG, 2003年01月, THEORETICAL AND APPLIED GENETICS, 106 (2), 303 - 310, 英語研究論文(学術雑誌)
To investigate the phylogenetic relationships in Pyrus and other species in the Rosaceae, structure of chloroplast DNA (cpDNA) in pear (Pyrus ussuriensis var. hondoensis (Nakai et Kikuchi) Rehder) was compared with that of tobacco in Solanaceae and those of Gramineous plants. Twenty-nine genes were mapped onto pear cpDNA by Southern hybridization. The gene order in pear cpDNA was the same as those for tobacco and Arabidopsis thaliana cpDNA, but differed from those for Gramineous plants which have large deletions (the regions including some genes) and inversions compared with pear and tobacco cpDNA. Also the structure of pear cpDNA was different from that of Lotus japonicus and Oenothera elata with a large inversion in the region between rps16 and rbcL. Three deletions were detected in the non-coding region between rbcL and accD in pear cpDNA when compared with tobacco cpDNA. Distribution of these deletions were surveyed by PCR in 20 Rosaceous plants including 11 Pyrus species from subfamily Maloideae, and 9 other species from subfamilies Prunoideae, Rosoideae, and Spiraeoideae. These deletions were found in all of the Rosaceous plants except 5 Prunus species, suggesting that the deletions occurred before the differentiation of Rosaceae, and additional alterations might occur in Prunus species.
International Society for Horticultural Science, 2002年, Acta Horticulturae, 587, 259 - 268, 英語[査読有り]
研究論文(国際会議プロシーディングス)
Genomic clones of 2.8 kb, 4.3 kb and 6.5 kb
for the S2-, S3- and S5-RNases of Japanese pear (Pyrus pyrifolia), respectively, were isolated and sequenced. Comparison of the 5’-flanking regions of these genes with the same region of the S4-RNase gene indicated that a highly similar region of approximately 200 bp exists in
the regions just upstream of the putative TATA boxes of th
[査読有り]
研究論文(学術雑誌)
植物の多様な花色や着色パターンがどのように調節されているかを,アントシアニン系色素を中心に,キンギョソウ,ペチュニア,トウモロコシなどの研究例から概説した。キンギョソウやトウモロコシはゲノム内にトランスポゾンを有するために多くの突然変異系統があり,アントシアニン合成系についても遺伝学的知見の蓄積があった。また分子生物学的解析により構造遺伝子ならびに制御遺伝子からなるアントシアニン合成系の発現制御についても解明されつつある。本稿ではトランスポゾンの構造上の特徴にも言及し,その挿入や切り出しによって斑入りを生じる易変性変異や花色発現パターンの変異が創出される分子機構を紹介した。またアントシアニン合成系の進化を考える上で一つの可能性として,構造遺伝子における遺伝子重複と構造変異,ならびに新たな制御因子による発現調節機構の獲得があることを示した。これまでの研究はキンギョソウ,ペチュニア,トウモロコシなど一部のモデル植物が中心であったが,分子生物学的手法の進歩と簡便化に伴い,今後はより広範な植物で構造遺伝子や制御因子の解析がすすみ,アントシアニン合成系の進化の全体像が明らかになると考える。
日本植物分類学会, 1999年, 植物分類,地理, 49 (2), 171 - 192, 日本語イワテヤマナシ(Pyrus ussuriensis var. aromatica)は東北地方に自生する野生ナシの1変種である.1940年頃までは利用されていたが,現在は地元でもほとんど知られておらず,消滅の恐れがある.筆者は遺伝資源としてのイワテヤマナシに注目し,1999年より北東北3県を網羅した探索調査を行ってきた.その結果1500本以上のナシ属植物が見つかり,その8割は北上山系に集中して分布していた.集団構造解析により真のイワテヤマナシ集団を推定し,保全単位や保全方法を検討した.またイワテヤマナシの起源を解明するため,中国大陸に自生する秋子ナシ(Pyrus ussuriensis)との系統関係を調査した.ところでイワテヤマナシはニホンナシにない様々な有用形質を持つ.ここでは芳香を取り上げ,香気分析や香気関連QTL座の決定,香りナシ育種について紹介
近畿作物・育種研究会, 2019年10月, 作物研究, 64, 1 - 9, 日本語[査読有り][招待有り]
日本に自生する香りナシ(梨)遺伝資源のイワテヤマナシ
日本香料協会, 2018年09月, 香料, 279, 59 - 64, 日本語[招待有り]
記事・総説・解説・論説等(学術雑誌)
ウスリーナシは中国北部において最も重要な栽培ナシである。ウスリーナシ栽培品種は中国北東部のPyrus ussuriensis Maxim.から派生したと考えられている。日本ではP. ussuriensisの2変種であるP. ussuriensis var. aromatica(イワテヤマナシ=ミチノクナシ)とvar. hondoensis(アオナシ)が本州北部と中部に自生している。イワテヤマナシの起源を明らかにするために40回以上の探索調査を日本と中国で行い、30カ所以上の自生地を確認した。これらの自生地は人為的な開発による消失と気候変動による森林劣化の危険性が高かった。保全単位の決定と保全管理をめざして、中国のP. ussuriensisと日本のイワテヤマナシにおける集団構造と遺伝的多様性について、形態形質と分子マーカーの両面から調査した。また集団
Japanese Society of Breeding, 2016年04月, Breeding Science, 66 (1), 90 - 99, 英語[査読有り][招待有り]
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