研究者紹介システム

石川 亮
イシカワ リョウ
大学院農学研究科 資源生命科学専攻
准教授
農学関係
Last Updated :2021/05/12

研究者情報

所属

  • 【主配置】

    大学院農学研究科 資源生命科学専攻
  • 【配置】

    農学部 資源生命科学科

学位

  • 博士(バイオサイエンス), 奈良先端科学技術大学院大学

授業科目

ジャンル

  • 環境・交通 / 農業

コメントテーマ

  • 植物育種学
  • 野生イネ
  • イネ栽培化過程の解明
  • 有用農業形質を支配する遺伝子の同定
  • 種子脱粒性

研究活動

研究キーワード

  • 植物育種学

研究分野

  • 環境・農学 / 遺伝育種科学

委員歴

  • 2020年12月 - 現在, Genetica, Editorial Board of Genetica
  • 2014年04月 - 2016年03月, 近畿作物育種学会, シンポジウム委員

受賞

  • 2018年09月 日本育種学会, 第134回講演会日本育種学会優秀発表賞 (2), Exomeと果実・葉形状にみる日本のウメの多様性

    沼口孝司, 赤木剛士, 北村祐人, 大江孝明, 石川亮, 石井尊生

    国内学会・会議・シンポジウム等の賞

  • 2018年09月 日本育種学会, 第134回講演会日本育種学会優秀発表賞, ジャポニカ型栽培イネ(日本晴)の非脱粒性に関与する新規遺伝子座の離層形成への効果

    杉山 昇平, 辻村 雄紀, 大塚 宏太郎, Htun, Than Myint, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2017年11月 公益財団法人 農学会, 第16回日本農学進歩賞, 栽培イネの成立に関与した遺伝子の同定と栽培化過程の解明

    石川亮

    国内学会・会議・シンポジウム等の賞

  • 2016年09月 日本育種学会, 第130回講演会日本育種学会優秀発表賞, Oryza rufipogonとインディカ型栽培イネ(カサラス)の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2014年09月 日本育種学会, 日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座の相互作用と離層形態

    井上 千鶴, Than Myint Htun, 井上 加奈子, 池田 健一, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2014年09月 日本育種学会, 第126回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用と離層形態

    井上 千鶴, Than Myint Htun, 井上 加奈子, 池田 健一, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2014年03月 日本育種学会, 第125回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用

    井上 千鶴, Than Myint Htun, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

論文

  • Yaddehige Priya Jayantha Amarasinghe, Rie Kuwata, Akinori Nishimura, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    Springer Science and Business Media LLC, 2020年12月, Rice, 13 (1), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koji Numaguchi, Takashi Akagi, Yuto Kitamura, Ryo Ishikawa, Takashige Ishii

    Wiley, 2020年11月08日, The Plant Journal, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Cristina C. Castillo, Dorian Q. Fuller

    Springer Science and Business Media LLC, 2020年08月, Archaeological and Anthropological Sciences, 12 (8), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Estimation of demographic history of Japanese and Taiwanese populations in Prunus mume using SSR marker genotypes

    Koji Numaguchi, Yuto Kitamura, Ryo Ishikawa, Takashige Ishii

    2020年, Koji Numaguchi, Yuto Kitamura, Ryo Ishikawa, Takashige Ishii, 65, 31 - 35, 英語

    [査読有り]

  • Spikelet opening under controlled transpiration conditions in rice

    Funa Ogawa, Ryo Ishikawa, Takashige Ishii

    2019年, Journal of Crop Research, 64, 31 - 35, 英語

    [査読有り]

    研究論文(学術雑誌)

  • QTL analysis for awnlessness using a backcross population between Oryza rufipogon and O. sativa IR36

    Yaddehige P, J. Amarasinghe, Rie Kuwata, Ryo Ishikawa, Takashige Ishii

    2019年, Journal of Crop Research, 64, 43 - 46, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koji Numaguchi, Shogo Ishio, Yuto Kitamura, Kentaro Nakamura, Ryo Ishikawa, Takashige Ishii

    Japanese Society for Horticultural Science, 2019年, The Horticulture Journal, 88 (2), 222 - 231, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Detection of a novel locus involved in non-seed-shattering behaviour of Japonica rice cultivar, Oryza sativa 'Nipponbare'

    Yuki Tsujimura, Shohei Sugiyama, Kotaro Otsuka, Than Myint Htun, Koji Numaguchi, Cristina Castillo, Takashi Akagi, Takashige Ishii, Ryo Ishikawa

    2019年, Theor. Appl. Genet., 英語

    [査読有り]

    研究論文(学術雑誌)

  • Arabidopsis FLL2 promotes liquid-liquid phase separation of polyadenylation complexes

    Xiaofeng Fang, Liang Wang, Ryo Ishikawa, Yaoxi Li, Marc Fiedler, Fuquan Liu, Grant Calder, Beth Rowan, Detlef Weigel, Pilong Li, Caroline Dean

    2019年, Nature, 569, 265 - 269, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashige Ishii, Ryo Ishikawa

    Cultivated rice (Oryza sativa L.) was domesticated from the Asian wild species, Oryza rufipogon Griff. Among morphological differences between them, one of the striking traits specific to cultivated rice is loss of seed shattering. In the early stage of rice domestication, the related traits of this character have been desirable for the ancient seed gatherers because it enhances the efficiency of seed collection. In this chapter, we propose that three morphological traits, closed panicle shape, non-seed shattering, and seed awning, played important roles in controlling the degree of seed dispersal. First, we reviewed domestication loci controlling the three traits. We then evaluated allele effects at these loci using reciprocal backcross populations between O. sativa Nipponbare and our standard wild accession of O. rufipogon W630. In the genetic background of cultivated rice, all the wild functional alleles were responsible for these domestication traits. On the other hand, cultivated non-functional alleles were not always associated with the drastic morphological changes in the genetic background of wild rice. Since ancient humans have selected cultivated-type mutants in natural wild populations, possible domestication process for the emergence of cultivated rice is discussed based on the effects of cultivated non-functional alleles.

    Springer Singapore, 2018年03月15日, Rice Genomics, Genetics and Breeding, 207 - 221, 英語

    [査読有り]

    論文集(書籍)内論文

  • Ryo Ishikawa, Masahide Iwata, Kenta Taniko, Gotaro Monden, Naoya Miyazaki, Chhourn Orn, Yuki Tsujimura, Shusaku Yoshida, Jian Feng Ma, Takashige Ishii

    Zinc (Zn) is one of the essential mineral elements for both plants and humans. Zn deficiency in human is one of the major causes of hidden hunger, a serious health problem observed in many developing countries. Therefore, increasing Zn concentration in edible part is an important issue for improving human Zn nutrition. Here, we found that an Australian wild rice O. meridionalis showed higher grain Zn concentrations compared with cultivated and other wild rice species. The quantitative trait loci (QTL) analysis was then performed to identify the genomic regions controlling grain Zn levels using backcross recombinant inbred lines derived from O. sativa 'Nipponbare' and O. meridionalis W1627. Four QTLs responsible for high grain Zn were detected on chromosomes 2, 9, and 10. The QTL on the chromosome 9 (named qGZn9), which showed the largest effect on grain Zn concentration was confirmed with the introgression line, which had a W1627 chromosomal segment covering the qGZn9 region in the genetic background of O. sativa 'Nipponbare'. Fine mapping of this QTL resulted in identification of two tightly linked loci, qGZn9a and qGZn9b. The candidate regions of qGZn9a and qGZn9b were estimated to be 190 and 950 kb, respectively. Furthermore, we also found that plants having a wild chromosomal segment covering qGZn9a, but not qGZn9b, is associated with fertility reduction. qGZn9b, therefore, provides a valuable allele for breeding rice with high Zn in the grains.

    PUBLIC LIBRARY SCIENCE, 2017年10月, PLOS ONE, 12 (10), e0187224, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Akinori Nishimura, Than Myint Htun, Ryo Nishioka, Yumi Oka, Yuki Tsujimura, Chizuru Inoue, Takashige Ishii

    Rice (Oryza sativa L.) is widely cultivated around the world and is known to be domesticated from its wild form, O. rufipogon. A loss of seed shattering is one of the most obvious phenotypic changes selected for during rice domestication. Previously, three seed-shattering loci, qSH1, sh4, and qSH3 were reported to be involved in non-shattering of seeds of Japonica-type cultivated rice, O. sativa cv. Nipponbare. In this study, we focused on non-shattering characteristics of O. sativa Indica cv. IR36 having functional allele at qSH1. We produced backcross recombinant inbred lines having chromosomal segments from IR36 in the genetic background of wild rice, O. rufipogon W630. Histological and quantitative trait loci analyses of abscission layer formation were conducted. In the analysis of quantitative trait loci, a strong peak was observed close to sh4. We, nevertheless, found that some lines showed complete abscission layer formation despite carrying the IR36 allele at sh4, implying that non-shattering of seeds of IR36 could be regulated by the combination of mutations at sh4 and other seed-shattering loci. We also genotyped qSH3, a recently identified seed-shattering locus. Lines that have the IR36 alleles at sh4 and qSH3 showed inhibition of abscission layer formation but the degree of seed shattering was different from that of IR36. On the basis of these results, we estimated that non-shattering of seeds in early rice domestication involved mutations in at least three loci, and these genetic materials produced in this study may help to identify novel seed-shattering loci.

    SPRINGER, 2017年04月, GENETICA, 145 (2), 201 - 207, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    Seed awning is one of the important traits for successful propagation in wild rice. During the domestication of rice by ancient humans, plants with awnless seeds may have been selected because long awns hindered collection and handling activities. To investigate domestication of awnless rice, QTL analysis for seed awning was first carried out using backcross recombinant inbred lines between Oryza sativa Nipponbare (recurrent parent) and O. rufipogon W630 (donor parent). Two strong QTLs were detected in the same regions as known major seed-awning loci, An-1 and RAE2. Subsequent causal mutation surveying and fine mapping confirmed that O. rufipogon W630 has functional alleles at both loci. The gene effects and interactions at these loci were examined using two backcross populations with reciprocal genetic backgrounds of O. sativa Nipponbare and O. rufipogon W630. As awn length in wild rice varied among seeds even in the same plant, awn length was measured based on spikelet position. In the genetic background of cultivated rice, the wild alleles at An-1 and RAE2 had awning effects, and plants having both wild homozygous alleles produced awns whose length was about 70% of those of the wild parent. On the other hand, in the genetic background of wild rice, the substitution of cultivated alleles at An-1 and RAE2 contributed little to awn length reduction. These results indicate that the domestication process of awnless seeds was complicated because many genes are involved in awn formation in wild rice.

    GENETICS SOC JAPAN, 2017年02月, GENES & GENETIC SYSTEMS, 92 (1), 21 - 26, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    Seed awning is one of the important traits for successful propagation in wild rice. During the domestication of rice by ancient humans, plants with awnless seeds may have been selected because long awns hindered collection and handling activities. To investigate domestication of awnless rice, QTL analysis for seed awning was first carried out using backcross recombinant inbred lines between Oryza sativa Nipponbare (recurrent parent) and O. rufipogon W630 (donor parent). Two strong QTLs were detected in the same regions as known major seed-awning loci, An-1 and RAE2. Subsequent causal mutation surveying and fine mapping confirmed that O. rufipogon W630 has functional alleles at both loci. The gene effects and interactions at these loci were examined using two backcross populations with reciprocal genetic backgrounds of O. sativa Nipponbare and O. rufipogon W630. As awn length in wild rice varied among seeds even in the same plant, awn length was measured based on spikelet position. In the genetic background of cultivated rice, the wild alleles at An-1 and RAE2 had awning effects, and plants having both wild homozygous alleles produced awns whose length was about 70% of those of the wild parent. On the other hand, in the genetic background of wild rice, the substitution of cultivated alleles at An-1 and RAE2 contributed little to awn length reduction. These results indicate that the domestication process of awnless seeds was complicated because many genes are involved in awn formation in wild rice.

    GENETICS SOC JAPAN, 2017年02月, GENES & GENETIC SYSTEMS, 92 (1), 21 - 26, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 大久保和男, 井上千鶴, 辻村雄紀, 石川亮

    Easy-shattering habit of the rice plant increases head loss in the Japanese head-feeding combine harvester, while the non-shattering habit increases processing loss in the conventional combine harvester. Thus, the performance of combine harvesters depends on the grain-shattering trait of the rice plant. To evaluate the effects of the grain-shattering trait on various losses at combine harvesting, we developed pairs of near-isogenic lines (NILs) for shattering habit by the selfing method. The NILs were derived from a cross between japonica rice cultivars, namely, “Asahi” and “Setokogane” “Asahi” has an easily shattering habit, while “Setokogane” has a hard-to-shatter habit. We developed eleven pairs of NILs. Alleles at qSH1 locus were found to be associated with either easily shattering or hard-to-shatter in the pairs of NILs. Grain-shattering habits of eleven hard-to-shatter lines were not significantly different from each other, but, those of some easily shattering lines were significantly different. This shows that the genetic background affected the grain-shattering degree in the lines with shattering. These data suggest that additional loci with minor effects on the grain-shattering habit may exist in the cross between “Asahi” and “Setokogane”. Additionally, the non-functional allele at qSH1 which involves the hard-to-shatter habit, may behave epistatic to other loci. Thus, the NILs would be used to conveniently evaluate the effects of the grain-shattering trait on various losses at combine harvesting and the genetic analysis of grain-shattering in japonica rice.

    Crop Science Society of Japan, 2017年, 日本作物学会紀事, 86 (4), 358 - 366, 中国語

    [査読有り]

    研究論文(学術雑誌)

  • Investigation of genetic loci controlling non-shattering behaviour in an Indica rice cultivar ‘IR36’. Journal of Crop Research 62: 19-23.

    Yuki Tsujimura, Chizuru Inoue, Than Mynt Htun, Yumi Oka, Takashige Ishii, Ryo Ishikawa

    2017年, Journal of Crop Research, 62, 19 - 23, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Identification of quantitative trait loci controlling floral morphology of rice using a backcross population between common cultivated rice, Oryza sativa and Asian wild rice, O. rufipogon

    Ryo Ishikawa, Takafumi Watabe, Ryo Nishioka, Pham Tien Thanh, Takashige Ishii

    2017年, American Journal of Plant Science, 8, 734 - 744, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Genetic survey of genomic regions responsible for panicle spreading in wild rice, Oryza rufipogon

    Ryo Nishioka, Hiromi Mikazuki, Than Myint Htun, Ryo Ishikawa, Takashige Ishii

    2016年, Journal of Crop Research, 61, 23 - 26, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Evaluation of shooting and rooting ability from nodes and ratooning ability using backcross recombinant inbred lines between Oryza rufipogon and O. sativa

    Mai Ikemoto, Tetsuya Ohyagi, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii

    2016年, Journal of Crop Research, 61, 13 - 17, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chhourn Orn, Rieko Shishido, Masahiro Akimoto, Ryo Ishikawa, Than Myint Htun, Ken-Ichi Nonomura, Yohei Koide, Men Sarom, Seng Vang, Sakhan Sophany, Ouk Makara, Takashige Ishii

    A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources.

    JAPANESE SOC BREEDING, 2015年12月, BREEDING SCIENCE, 65 (5), 430 - 437, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chizuru Inoue, Than Myint Htun, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa

    Loss of seed shattering was one of the key phenotypic changes selected for in the domestication of many crop species. Asian cultivated rice, Oryza sativa L., was domesticated from its wild ancestor, 0. rufipogon, and three seed-shattering loci, qSH1, sh4 and qSH3, have been reported to be involved in the loss of seed shattering in cultivated rice. Here, we analysed the seed-shattering behaviour of wild rice using introgression lines carrying the cultivated alleles from 0. sativa Nipponbare in the genetic background of wild rice, 0. rufipogon W630. We first carried out fine mapping of the qSH3 region and found that the qSH3 locus is localized in an 850-kb region on chromosome 3. We then analysed the effects of the Nipponbare alleles at sh4 and qSH3 on seed-shattering behaviour in wild rice, as a mutation at qSH1 was not commonly found in rice cultivars. Seed-shattering behaviour did not change in the two types of introgression line independently carrying the Nipponbare-homozygous alleles at sh4 or qSH3 in the genetic background of wild rice. However, the introgression lines having the Nipponbare-homozygous alleles at both sh4 and qSH3 showed a reduction in the degree of seed shattering. Histological and scanning electron microscopy analyses revealed that abscission layer formation was inhibited around the vascular bundles in these lines. Since the qSH3 region, as well as sh4, has been shown to be under artificial selection, the interaction of mutations at these two loci may have played a role in the initial loss of seed shattering during rice domestication.

    GENETICS SOC JAPAN, 2015年02月, GENES & GENETIC SYSTEMS, 90 (1), 1 - 9, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagaya, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori

    The endosperm of cereal grains represents the most important source of human nutrition. In addition, the endosperm provides many investigatory opportunities for biologists because of the unique processes that occur during its ontogeny, including syncytial development at early stages. Rice endospermless 1 (enl1) develops seeds lacking an endosperm but carrying a functional embryo. The enl1 endosperm produces strikingly enlarged amoeboid nuclei. These abnormal nuclei result from a malfunction in mitotic chromosomal segregation during syncytial endosperm development. The molecular identification of the causal gene revealed that ENL1 encodes an SNF2 helicase family protein that is orthologous to human Plk1-Interacting Checkpoint Helicase (PICH), which has been implicated in the resolution of persistent DNA catenation during anaphase. ENL1-Venus (enhanced yellow fluorescent protein (YFP)) localizes to the cytoplasm during interphase but moves to the chromosome arms during mitosis. ENL1-Venus is also detected on a thread-like structure that connects separating sister chromosomes. These observations indicate the functional conservation between PICH and ENL1 and confirm the proposed role of PICH. Although ENL1 dysfunction also affects karyokinesis in the root meristem, enl1 plants can grow in a field and set seeds, indicating that its indispensability is tissue-dependent. Notably, despite the wide conservation of ENL1/PICH among eukaryotes, the loss of function of the ENL1 ortholog in Arabidopsis (CHR24) has only marginal effects on endosperm nuclei and results in normal plant development. Our results suggest that ENL1 is endowed with an indispensable role to secure the extremely rapid nuclear cycle during syncytial endosperm development in rice.

    WILEY-BLACKWELL, 2015年01月, PLANT JOURNAL, 81 (1), 1 - 12, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagaya, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori

    The endosperm of cereal grains represents the most important source of human nutrition. In addition, the endosperm provides many investigatory opportunities for biologists because of the unique processes that occur during its ontogeny, including syncytial development at early stages. Rice endospermless 1 (enl1) develops seeds lacking an endosperm but carrying a functional embryo. The enl1 endosperm produces strikingly enlarged amoeboid nuclei. These abnormal nuclei result from a malfunction in mitotic chromosomal segregation during syncytial endosperm development. The molecular identification of the causal gene revealed that ENL1 encodes an SNF2 helicase family protein that is orthologous to human Plk1-Interacting Checkpoint Helicase (PICH), which has been implicated in the resolution of persistent DNA catenation during anaphase. ENL1-Venus (enhanced yellow fluorescent protein (YFP)) localizes to the cytoplasm during interphase but moves to the chromosome arms during mitosis. ENL1-Venus is also detected on a thread-like structure that connects separating sister chromosomes. These observations indicate the functional conservation between PICH and ENL1 and confirm the proposed role of PICH. Although ENL1 dysfunction also affects karyokinesis in the root meristem, enl1 plants can grow in a field and set seeds, indicating that its indispensability is tissue-dependent. Notably, despite the wide conservation of ENL1/PICH among eukaryotes, the loss of function of the ENL1 ortholog in Arabidopsis (CHR24) has only marginal effects on endosperm nuclei and results in normal plant development. Our results suggest that ENL1 is endowed with an indispensable role to secure the extremely rapid nuclear cycle during syncytial endosperm development in rice.

    WILEY-BLACKWELL, 2015年01月, PLANT JOURNAL, 81 (1), 1 - 12, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, Ryo Ishikawa

    Asian cultivated rice Oryza sativa L. was domesticated from its wild ancestor, O. rufipogon. During domestication, the cultivated rice lost its seed-shattering behaviour. Previous studies have shown that two major quantitative trait loci (QTLs; qSH1 and sh4) are responsible for the seed-shattering degree. Here, we produced introgression lines carrying non-functional alleles from O. sativa 'Nipponbare' at the two major QTLs in the genetic background of wild rice O. rufipogon W630, and examined the effects of the two QTLs on seed shattering and abscission layer formation. The introgression lines, with Nipponbare alleles at either or both loci, showed complete or partial abscission layer formation, respectively, indicating that other unknown loci might be involved in enhancing seed shattering in wild rice. We detected a single QTL named qSH3 regulating seed-shattering degree using an F-2 population between Nipponbare and the introgression line carrying Nipponbare alleles at the two QTLs. Although we generated an introgression line for qSH3 alone, no effects on seed shattering were observed. However, a significant effect on seed-shattering degree was observed for the introgression line carrying Nipponbare alleles at qSH3 and the two QTLs, suggesting an important role of qSH3 on seed shattering in coordination with the two QTLs.

    JAPANESE SOC BREEDING, 2014年09月, BREEDING SCIENCE, 64 (3), 199 - 205, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Evaluation of genetic variation among rice local and modern varieties in Cambodia

    Orn Chhourn, Ryo Ishikawa, Sakhan Sophany, Ouk Makara, Takashige Ishii

    2014年, Journal of Crop research, 59, 37 - 41, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashige Ishii, Koji Numaguchi, Kotaro Miura, Kentaro Yoshida, Pham Thien Thanh, Than Myint Htun, Masanori Yamasaki, Norio Komeda, Takashi Matsumoto, Ryohei Terauchi, Ryo Ishikawa, Motoyuki Ashikari

    Reduction in seed shattering was an important phenotypic change during cereal domestication(1,2). Here we show that a simple morphological change in rice panicle shape, controlled by the SPR3 locus, has a large impact on seed-shedding and pollinating behaviors. In the wild genetic background of rice, we found that plants with a cultivated-like type of closed panicle had significantly reduced seed shedding through seed retention. In addition, the long awns in closed panicles disturbed the free exposure of anthers and stigmas on the flowering spikelets, resulting in a significant reduction of the outcrossing rate. We localized the SPR3 locus to a 9.3-kb genomic region, and our complementation tests suggest that this region regulates the liguleless gene (OsLG1). Sequencing analysis identified reduced nucleotide diversity and a selective sweep at the SPR3 locus in cultivated rice. Our results suggest that a closed panicle was a selected trait during rice domestication.

    NATURE PUBLISHING GROUP, 2013年04月, NATURE GENETICS, 45 (4), 462 - 465, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashige Ishii, Koji Numaguchi, Kotaro Miura, Kentaro Yoshida, Pham Thien Thanh, Than Myint Htun, Masanori Yamasaki, Norio Komeda, Takashi Matsumoto, Ryohei Terauchi, Ryo Ishikawa, Motoyuki Ashikari

    Reduction in seed shattering was an important phenotypic change during cereal domestication(1,2). Here we show that a simple morphological change in rice panicle shape, controlled by the SPR3 locus, has a large impact on seed-shedding and pollinating behaviors. In the wild genetic background of rice, we found that plants with a cultivated-like type of closed panicle had significantly reduced seed shedding through seed retention. In addition, the long awns in closed panicles disturbed the free exposure of anthers and stigmas on the flowering spikelets, resulting in a significant reduction of the outcrossing rate. We localized the SPR3 locus to a 9.3-kb genomic region, and our complementation tests suggest that this region regulates the liguleless gene (OsLG1). Sequencing analysis identified reduced nucleotide diversity and a selective sweep at the SPR3 locus in cultivated rice. Our results suggest that a closed panicle was a selected trait during rice domestication.

    NATURE PUBLISHING GROUP, 2013年04月, NATURE GENETICS, 45 (4), 462 - 465, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Phuong Dang Thai Phan, Hiroki Kageyama, Ryo Ishikawa, Takashige Ishii

    The Asian wild rice, Oryza rufipogon, has partial outcrossing behavior and shows high levels of genetic variation. To estimate an accurate outcrossing rate of annual form of O. rufipogon, two backcross lines (Lines R1 and R2) between Oryza saliva Nipponbare and O. rufipogon W630 were examined under field conditions. A chromosome survey confirmed that these lines had wild chromosomal segments at more than 92% of marker loci. As for the traits of glume, stamen and pistil, Line R1 showed similar floral morphology as that of O. rufipogon W630, whereas Line R2 had larger glumes. In 2005, 2006 and 2008, a total of 22 backcross plants were planted in the middle of wild rice plots. The successive progenies of each plant were examined using microsatellite markers that could clearly detect self-pollination and outcrossing. The outcrossing rates of Line R1 plants ranged from 4.04% to 25.50% with an average of 10.20%. This indicates that cross-pollination of wild rice is a chance event affected by many environmental factors. The outcrossing rates of Line R2 plants also varied, however, no significant difference was observed between the averages of Lines RI and R2, suggesting that the glume sizes are not critical for outcrossing ability.

    JAPANESE SOC BREEDING, 2012年09月, BREEDING SCIENCE, 62 (3), 256 - 262, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashige Ishii, Takashi Hiraoka, Tomoyuki Kanzaki, Masahiro Akimoto, Rieko Shishido, Ryo Ishikawa

    Cultivated rice (Oryza sativa L.) is derived from Asian wild rice (Oryza rufipogon Griff). Vietnamese local varieties and wild natural populations in Vietnam and Myanmar were examined to evaluate the levels of genetic variation in cultivated and wild rice. In total, 222 Vietnamese local varieties were analyzed with ten microsatellite markers. Using marker genotype and gene diversity data, the local varieties were differentiated based on geographical distribution, cropping season, and human preference. A total of 976 wild plants were collected at six natural sites of wild populations (three each in Myanmar and Vietnam), and the degrees of variation among populations were analyzed with five microsatellite markers. Phylogenetic analyses revealed wide genetic differentiation among wild populations. The diversity values detected in a single wild population in Vietnam were higher than those in whole Vietnamese local varieties. These results indicate that wild rice has much greater genetic variation than cultivated rice.

    SPRINGER, 2011年12月, RICE, 4 (3-4), 170 - 177, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Mayumi Aoki, Ken-ichi Kurotani, Shuji Yokoi, Tomoko Shinomura, Makoto Takano, Ko Shimamoto

    Many plants require circadian clock and light information for the photoperiodic control of flowering. In Arabidopsis, a long-day plant (LDP), flowering is triggered by the circadian clock-controlled expression of CONSTANS (CO) and light stabilization of the CO protein to induce FT (FLOWERING LOCUS T). In rice, a short-day plant (SDP), the CO ortholog Heading date 1 (Hd1) regulates FT ortholog Hd3a, but regulation of Hd3a by Hd1 differs from that in Arabidopsis. Here, we report that phytochrome B (phyB)-mediated suppression of Hd3a is a primary cause of long-day suppression of flowering in rice, based on the three complementary discoveries. First, overexpression of Hd1 causes a delay in flowering under SD conditions and this effect requires phyB, suggesting that light modulates Hd1 control of Hd3a transcription. Second, a single extension of day length decreases Hd3a expression proportionately with the length of daylight. Third, Hd1 protein levels in Hd1-overexpressing plants are not altered in the presence of light. These results also suggest that phyB-mediated suppression of Hd3a expression is a component of the molecular mechanism for critical day length in rice.

    SPRINGER HEIDELBERG, 2011年06月, MOLECULAR GENETICS AND GENOMICS, 285 (6), 461 - 470, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Takayuki Ohnishi, Yuki Kinoshita, Mitsugu Eiguchi, Nori Kurata, Tetsu Kinoshita

    Summary In angiosperms, interspecific crosses often display hybrid incompatibilities that are manifested as under-proliferation or over-proliferation of endosperm. Recent analyses using crosses between Arabidopsis thaliana and its related species with different ploidy levels have shown that interspecific hybridization causes delayed developmental transition and increased mitotic activity in the endosperm. In this study, we investigated endosperm development in interspecific crosses between diploid Oryza species. In a cross between female O. sativa and male O. punctata, we found that the hybrid endosperm was reduced in size and this cross was associated with precocious developmental transition. By contrast, the cross between O. sativa and O. longistaminata generated enlarged hybrid endosperm at the mid-point of seed development and this cross was associated with delayed developmental transition. Subsequently, the hybrid endosperm displayed a shriveled appearance at the seed maturation stage. We found that the accumulation of storage products and the expression patterns of several marker genes were also altered in the hybrid endosperm. By contrast, the rate of syncytial mitotic nuclear divisions was not significantly affected. The gene OsMADS87 showed a maternal origin-specific expression pattern in rice endosperm, in contrast to its Arabidopsis homologue PHERES1, which shows paternal origin-specific expression. OsMADS87 expression was decreased or increased depending on the type of developmental transition change in the hybrid rice endosperm. Our results indicate that one of the interspecies hybridization barriers in Oryza endosperm is mediated by precocious or delayed developmental alterations and de-regulation of OsMADS87, without change to the rate of syncytial mitotic nuclear division in the hybrid endosperm. © 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.

    2011年03月, Plant Journal, 65 (5), 798 - 806, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Pham Thien Thanh, Phuong Dang Thai Phan, Naoki Mori, Ryo Ishikawa, Takashige Ishii

    Backcross populations at advanced generations are useful in evaluating the agronomic traits from unadapted germplasms, such as wild species. In this study, we generated 159 backcross recombinant inbred lines (BRILs) at the BC2F8 generation between Oryza saliva Nipponbare (a recurrent parent) and O. rufipogon W630 (a donor parent). These BRILs were found to have nearly homozygous genome constitutions based on the marker genotypes at 180 SSR loci covering whole chromosomes. Since their selfed progenies have almost identical genotypes in each line, they were further used for QTL analysis of drought tolerance. Out of six QTLs detected, three were found to have wild alleles with favorable effects on drought tolerance. The present BRILs with known marker data can be applied to more QTL analyses of traits specific to wild species.

    JAPANESE SOC BREEDING, 2011年03月, BREEDING SCIENCE, 61 (1), 76 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Temporal changes of seed shattering degree of substitution lines having non-shattering alleles from cultivated rice (Oryza sativa) in the genetic background of wild rice (O. rufipogon)

    Than Myint Htun, Takashige Ishii, Ryo Ishikawa

    2011年, Journal of Crop research, 56, 39 - 44, 英語

    [査読有り]

    研究論文(学術雑誌)

  • QTL analysis for submergence avoidance using backcross recombinant inbred lines between Oryza sativa and O. rufipogon.

    Phuong Dang, Thai Phan, Pham Thien Thanh, Naoki Mori, Ryo Ishikawa, Takashige Ishii

    2011年, Journal of Crop research, 56, 45 - 49, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    In the near future, global average temperature is expected to increase due to the accumulation of greenhouse gases, and increased temperatures will cause severe sterility in many crop species. In rice, since wild species show high genetic variation, they may have the potential to improve the flowering characters of cultivars. In this study, we investigated flowering characters under natural conditions by comparing an Asian wild rice accession of Oryza rufipogon W630 (originated from Myanmar) with a Japanese rice cultivar, O. sativa Japonica cv. Nipponbare. Further, QTL analysis for days to heading (DH) and spikelet opening time (SOT: the time of day when the spikelet opens) was carried out using BC2F8 backcross population derived from the cross between them. Regarding DH, four QTLs were detected, and two of them were found to have wild alleles with strong effects leading to longer days to heading during the Japanese summer. These wild alleles may be used to produce late-heading cultivars that do not flower during the high summer temperatures anticipated in the future. As for SOT, two parameters of SOTb (beginning time when the first spikelet opens) and SOTm (median time when 50% of the spikelets open) were recorded and the time differences from Nipponbare were investigated. Two QTLs on chromosomes 5 and 10 and two QTLs on chromosomes 4 and 5 were detected for SOTb and SOTm, respectively. The wild alleles were responsible for early spikelet opening time at all loci. If the wild alleles detected in this study have the same effects in the genetic background of other cultivars, they will be very useful in producing early-flowering rice cultivars that complete fertilization in the morning before the temperature rises.

    GENETICS SOC JAPAN, 2010年08月, GENES & GENETIC SYSTEMS, 85 (4), 273 - 279, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Pham Thien Thanh, Naoto Nimura, Than Myint Htun, Masanori Yamasaki, Takashige Ishii

    It is known that the common cultivated rice (Oryza sativa) was domesticated from Asian wild rice, O. rufipogon. Among the morphological differences between them, loss of seed shattering is one of the striking characters specific for the cultivated forms. In order to understand the genetic control on shattering habit, QTL analysis was carried out using BC2F1 backcross population between O. sativa cv. Nipponbare (a recurrent parent) and O. rufipogon acc. W630 (a donor parent). As a result, two strong QTLs were detected on chromosomes 1 and 4, and they were found to be identical to the two major seed-shattering loci, qSH1 and sh4, respectively. The allelic interaction at these loci was further examined using two sets of backcross populations having reciprocal genetic backgrounds, cultivated and wild. In the genetic background of cultivated rice, the wild qSH1 allele has stronger effect on seed shattering than that of sh4. In addition, the wild alleles at both qSH1 and sh4 loci showed semi-dominant effects. On the other hand, in the genetic background of wild rice, non-shattering effects of Nipponbare alleles at both loci were examined to inspect rice domestication from a viewpoint of seed shattering. It was serendipitous that the backcross plants individually having Nipponbare homozygous alleles at either shattering locus (qSH1 or sh4) shed all the seeds. This fact strongly indicates that the non-shattering behavior was not obtained by a single mutation in the genetic background of wild rice. Probably, some other minor genes are still associated with the formation or activation of abscission layer, which enhance the seed shattering.

    GENETICS SOC JAPAN, 2010年08月, GENES & GENETIC SYSTEMS, 85 (4), 265 - 271, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Tomoko Shinomura, Makoto Takano, Ko Shimamoto

    A short exposure to light during relative night (night break; NB) delays flowering in the short day plant rice. NB acts by downregulating Heading date 3a (Hd3a) expression. Because phytochrome B mutants do not respond to NB and their flowering time is not affected even under NB conditions, phyB is required for the suppression of Hd3a expression. The effect of NB is quantitatively controlled by light quality and by either light intensity or duration. However, the molecular mechanisms that regulate these interactions are poorly understood. Here, we examine the roles of phytochromes in the regulation of Hd3a transcription under NB conditions using monochromatic red, far-red and blue light. Red and blue light downregulated Hd3a expression, but far-red light NB did not. The effect of red light NB on Hd3a is dependent on photon fluence and is restored by subsequent far-red light irradiation. Our results suggest that quantitative effect of light on flowering in rice NB is mediated by the regulation of Hd3a transcription by phyB.

    GENETICS SOC JAPAN, 2009年04月, GENES & GENETIC SYSTEMS, 84 (2), 179 - 184, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Evaluation of genetic diversity among rice local varieties in Vietnam

    Takashi Hiraoka, Nobuyuki Ikeda, Ryo Ishikawa, Takashige Ishii

    2009年, Journal of Crop Research, 54, 125 - 130, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Molecular basis of night break effect on the photoperiodic control of flowering in rice

    Ishikawa R, Yokoi S, Shimamoto K

    8, 2006年07月01日, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme., 51 (8), 933 - 942, 日本語

    [査読有り]

  • 野生イネの種子脱粒性、穂の開帳性、頴の色を支配する遺伝子のマッピング

    石井 尊生, 崎山 弘樹, 石川 亮, 上島 脩志

    2006年, 近畿作育研究, 51, 23 - 26, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • R Ishikawa, S Tamaki, S Yokoi, N Inagaki, T Shinomura, M Takano, K Shimamoto

    A short exposure to light in the middle of the night causes inhibition of flowering in short-day plants. This phenomenon is called night break (NB) and has been used extensively as a tool to study the photoperiodic control of flowering for many years. However, at the molecular level, very little is known about this phenomenon. In rice (Oryza sativa), 10 min of light exposure in the middle of a 14-h night caused a clear delay in flowering. A single NB strongly suppressed the mRNA of Hd3a, a homolog of Arabidopsis thaliana FLOWERING LOCUS T (FT), whereas the mRNAs of OsGI and Hd1 were not affected. The NB effect on Hd3a mRNA was maximal in the middle of the 14-h night. The phyB mutation abolished the NB effect on flowering and Hd3a mRNA, indicating that the NB effect was mediated by phytochrome B. Because expression of the other FT-like genes was very low and not appreciably affected by NB, our results strongly suggest that the suppression of Hd3a mRNA is the principal cause of the NB effect on flowering in rice.

    AMER SOC PLANT BIOLOGISTS, 2005年12月, PLANT CELL, 17 (12), 3326 - 3336, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • 熱帯アジアにおける栽培イネから野生イネへの遺伝子流動について

    谷尾侑香, ORN Chhourn, 秋本正博, 宍戸理恵子, HTUN Than Myint, PHAN Phuong, Dang Thai, 野々村賢一, 小出陽平, 石川亮, 石井尊生

    2018年09月22日, 育種学研究, 20, 131, 日本語

  • オーストラリアの野生イネOryza meridionalisを利用したイネ種子亜鉛濃度を向上させる遺伝子座の検出

    石川亮

    2018年, NBRP RICE Newsletter, 1, 5 - 6, 日本語

    [招待有り]

    記事・総説・解説・論説等(その他)

  • 野生イネと栽培イネの間で検出されたアルミニウムストレス耐性を支配する遺伝子座qAl1の遺伝学的解析

    和田もりえ, 岩田将英, 横正健剛, 吉田周作, THANH Pham Thien, 石井尊生, MA J, 石川亮

    2016年03月21日, 育種学研究, 18, 202, 日本語

  • Ryo Ishikawa

    The F1000.com, 2015年03月, F1000 Prime, 1, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • Ryo Ishikawa

    The F1000.com, 2014年07月, F1000 Prime, 5, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • Jian-Yong Li, Jiping Liu, Dekun Dong, Xiaomin Jia, Susan R. McCouch, Leon V. Kochian

    Aluminum (Al) toxicity is a major constraint for crop production on acid soils which compose similar to 40% of arable land in the tropics and subtropics. Rice is the most Al-tolerant cereal crop and offers a good model for identifying Al tolerance genes and mechanisms. Here we investigated natural variation in the rice Nramp aluminum transporter (NRAT1) gene encoding a root plasma membrane Al uptake transporter previously hypothesized to underlie a unique Al tolerance mechanism. DNA sequence variation in the NRAT1 coding and regulatory regions was associated with changes in NRAT1 expression and NRAT1 Al transport properties. These sequence changes resulted in significant differences in Al tolerance that were found to be associated with changes in the Al content of root cell wall and cell sap in 24 representative rice lines from a rice association panel. Expression of the tolerant OsNRAT1 allele in yeast resulted in higher Al uptake than did the sensitive allele and conferred greater Al tolerance when expressed in transgenic Arabidopsis. These findings indicate that NRAT1 plays an important role in rice Al tolerance by reducing the level of toxic Al in the root cell wall and transporting Al into the root cell, where it is ultimately sequestered in the vacuole. Given its ability to enhance Al tolerance in rice and Arabidopsis, this work suggests that the NRAT1 gene or its orthologs may be useful tools for enhancing Al tolerance in a wide range of plant species.

    NATL ACAD SCIENCES, 2014年04月, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111 (17), 6503 - 6508, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • Kiyosumi Hori, Eri Ogiso-Tanaka, Kazuki Matsubara, Utako Yamanouchi, Kaworu Ebana, Masahiro Yano

    The alteration of photoperiod sensitivity has let breeders diversify flowering time in Oryza sativa (rice) and develop cultivars adjusted to a range of growing season periods. Map-based cloning revealed that the rice flowering-time quantitative trait locus (QTL) Heading date 16 (Hd16) encodes a casein kinase-I protein. One non-synonymous substitution in Hd16 resulted in decreased photoperiod sensitivity in rice, and this substitution occurred naturally in an old rice cultivar. By using near-isogenic lines with functional or deficient alleles of several rice flowering-time genes, we observed significant digenetic interactions between Hd16 and four other flowering-time genes (Ghd7, Hd1, DTH8 and Hd2). In a near-isogenic line with the weak-photoperiod-sensitivity allele of Hd16, transcription levels of Ehd1, Hd3a, and RFT1 increased under long-day conditions, and transcription levels of Hd3a and RFT1 decreased under short-day conditions. Expression analysis under continuous light and dark conditions showed that Hd16 was not likely to be associated with circadian clock regulation. Biochemical characterization indicated that the functional Hd16 recombinant protein specifically phosphorylated Ghd7. These results demonstrate that Hd16 acts as an inhibitor in the rice flowering pathway by enhancing the photoperiod response as a result of the phosphorylation of Ghd7.

    WILEY-BLACKWELL, 2013年10月, PLANT JOURNAL, 76 (1), 36 - 46, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • Ryo Ishikawa

    The F1000.com, 2013年10月, F1000 Prime, 9 (6), 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • Ryo Fujimoto, Taku Sasaki, Ryo Ishikawa, Kenji Osabe, Takahiro Kawanabe, Elizabeth S. Dennis

    Natural variation is defined as the phenotypic variation caused by spontaneous mutations. In general, mutations are associated with changes of nucleotide sequence, and many mutations in genes that can cause changes in plant development have been identified. Epigenetic change, which does not involve alteration to the nucleotide sequence, can also cause changes in gene activity by changing the structure of chromatin through DNA methylation or histone modifications. Now there is evidence based on induced or spontaneous mutants that epigenetic changes can cause altering plant phenotypes. Epigenetic changes have occurred frequently in plants, and some are heritable or metastable causing variation in epigenetic status within or between species. Therefore, heritable epigenetic variation as well as genetic variation has the potential to drive natural variation.

    MDPI AG, 2012年08月, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13 (8), 9900 - 9922, 英語

    [査読有り]

    書評論文,書評,文献紹介等

  • Ryo Fujimoto, Taku Sasaki, Ryo Ishikawa, Kenji Osabe, Takahiro Kawanabe, Elizabeth S. Dennis

    Natural variation is defined as the phenotypic variation caused by spontaneous mutations. In general, mutations are associated with changes of nucleotide sequence, and many mutations in genes that can cause changes in plant development have been identified. Epigenetic change, which does not involve alteration to the nucleotide sequence, can also cause changes in gene activity by changing the structure of chromatin through DNA methylation or histone modifications. Now there is evidence based on induced or spontaneous mutants that epigenetic changes can cause altering plant phenotypes. Epigenetic changes have occurred frequently in plants, and some are heritable or metastable causing variation in epigenetic status within or between species. Therefore, heritable epigenetic variation as well as genetic variation has the potential to drive natural variation.

    MDPI AG, 2012年08月, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13 (8), 9900 - 9922, 英語

    [査読有り]

    書評論文,書評,文献紹介等

  • Kenji Osabe, Takahiro Kawanabe, Taku Sasaki, Ryo Ishikawa, Keiichi Okazaki, Elizabeth S. Dennis, Tomohiko Kazama, Ryo Fujimoto

    An allopolyploid is an individual having two or more complete sets of chromosomes derived from different species. Generation of allopolyploids might be rare because of the need to overcome limitations such as co-existing populations of parental lines, overcoming hybrid incompatibility, gametic non-reduction, and the requirement for chromosome doubling. However, allopolyploids are widely observed among plant species, so allopolyploids have succeeded in overcoming these limitations and may have a selective advantage. As techniques for making allopolyploids are developed, we can compare transcription, genome organization, and epigenetic modifications between synthesized allopolyploids and their direct parental lines or between several generations of allopolyploids. It has been suggested that divergence of transcription caused either genetically or epigenetically, which can contribute to plant phenotype, is important for the adaptation of allopolyploids.

    MDPI, 2012年07月, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13 (7), 8696 - 8721, 英語

    [査読有り]

    書評論文,書評,文献紹介等

  • Kenji Osabe, Takahiro Kawanabe, Taku Sasaki, Ryo Ishikawa, Keiichi Okazaki, Elizabeth S. Dennis, Tomohiko Kazama, Ryo Fujimoto

    An allopolyploid is an individual having two or more complete sets of chromosomes derived from different species. Generation of allopolyploids might be rare because of the need to overcome limitations such as co-existing populations of parental lines, overcoming hybrid incompatibility, gametic non-reduction, and the requirement for chromosome doubling. However, allopolyploids are widely observed among plant species, so allopolyploids have succeeded in overcoming these limitations and may have a selective advantage. As techniques for making allopolyploids are developed, we can compare transcription, genome organization, and epigenetic modifications between synthesized allopolyploids and their direct parental lines or between several generations of allopolyploids. It has been suggested that divergence of transcription caused either genetically or epigenetically, which can contribute to plant phenotype, is important for the adaptation of allopolyploids.

    MDPI, 2012年07月, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13 (7), 8696 - 8721, 英語

    [査読有り]

    書評論文,書評,文献紹介等

  • フィトクロムによるHd1を介したフロリゲン遺伝子Hd3aの転写制御と日長認識機構

    石川亮, 青木麻由美, 黒谷賢一, 横井修司, 篠村知子, 高野誠, 島本功

    2011年03月29日, 育種学研究, 13, 273, 日本語

  • エピジェネティクスが制御する植物の形質

    藤本 龍, 石川 亮

    2010年, 育種学研究, 12, 123 - 131, 日本語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • Ryo Ishikawa, Tetsu Kinoshita

    In many organisms, the genomes of individual species are isolated by a range of reproductive barriers that act before or after fertilization. Successful mating between species results in the presence of different genomes within a cell (hybridization), which can lead to incompatibility in cellular events due to adverse genetic interactions. In addition to such genetic interactions, recent studies have shown that the epigenetic control of the genome, silencing of transposons, control of non-additive gene expression and genomic imprinting might also contribute to reproductive barriers in plant and animal species. These genetic and epigenetic mechanisms play a significant role in the prevention of gene flow between species. In this review, we focus on aspects of epigenetic control related to hybrid incompatibility during species hybridization, and also consider key mechanism(s) in the interaction between different genomes.

    2009年07月, Molecular Plant, 2 (4), 589 - 599, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • Tetsu Kinoshita, Yoko Ikeda, Ryo Ishikawa

    Maternally and paternally derived chromosomes might be expected to contribute equally to the various cellular and developmental processes in placental mammals and flowering plants. However, this is not true even in the case of the self-pollinated plant, Arabidopsis, which has identical DNA sequences in both parental genomes. The reason for this is that some genes, called "imprinted genes", are expressed exclusively from paternally or maternally inherited chromosomes. As a result, parental chromosomes express a distinct set of genes and play different roles in biological processes. Here, we review and compare roles of genomic imprinting in flowering plants and placental mammals. © 2008 Elsevier Ltd. All rights reserved.

    2008年12月, Seminars in Cell and Developmental Biology, 19 (6), 574 - 579, 英語

    [査読有り][招待有り]

    書評論文,書評,文献紹介等

  • 石川亮, 永口貢, 池田陽子, 倉田のり, 木下哲

    2008年03月15日, 日本植物生理学会年会要旨集, 49th, 225, 日本語

  • 木下 哲, 池田 陽子, 石川 亮

    2008年, 植物の生長調節, 43, 29 - 34, 日本語

    [査読有り][招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)

  • ~温故知新~ 光中断の実験から植物の光周性による開花の分子機構に迫る

    石川 亮, 横井 修司, 島本 功

    2006年, 蛋白質 核酸 酵素, 551, 993 - 942, 日本語

    [査読有り][招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)

書籍等出版物

  • アフロ・ユーラシアの考古植物学

    石川亮, 杉山昇平, 辻村雄紀, 沼口孝司, クリスティーナ・コボ・カスティヨ, 石井尊生

    その他, クバプロ, 2019年05月, 日本語

    学術書

  • Rice Genomics, Genetics and Breeding - Domestication loci controlling panicle shape, seed shattering and seed awning

    Takashige Ishii, Ryo Ishikawa

    その他, Springer, 2018年, 英語

    学術書

  • Rice Genomics, Genetics and Breeding - Domestication loci controlling panicle shape, seed shattering and seed awning

    Takashige Ishii, Ryo Ishikawa

    その他, Springer, 2017年, 英語

    学術書

  • DNA methylation; principles, mechanisms and challenges

    Kenji Osabe, Taku Sasaki, Ryo Ishikawa, Ryo Fujimoto

    その他, Nova Science, 2012年, 英語

    学術書

講演・口頭発表等

  • Target capture法に基づく日本と中国のウメの集団構造および系統関係について

    沼口孝司, 赤木剛士, 北村祐人, 石川亮, 石井尊生

    園芸学会 平成30年度秋季大会, 2019年03月, 日本語, 園芸学会, 明治大学, 国内会議

    口頭発表(一般)

  • QTL analysis for awnlessness using a backcross population between Oryza rufipogon and O. sativa IR36

    Priya J.A. Yaddehige, Rie Kuwata, Ryo Ishikawa, Takashige Ishii

    近畿作物・育種研究会 第186回例会, 2018年11月, 英語, 近畿作物・育種研究会, 京都大学, 国内会議

    口頭発表(一般)

  • 野生イネOryza meridionalisを用いて同定されたイネ種子亜鉛濃度を支配するqGZn9は連鎖した2つの遺伝子座によって制御される

    宮崎直哉, 谷古健太, 岩田将英, 門田剛太郎, Orn Chhourn, 吉田周作, 石井尊生, 馬建鋒, 石川亮

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    口頭発表(一般)

  • 穂の開帳性が栽培イネ(Oryza sativa)の穂形態に与える影響について

    窪田芳生, 西岡諒, 石井尊生, 石川亮

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    ポスター発表

  • 熱帯アジアにおける栽培イネから野生イネへの遺伝子流動について

    谷尾侑香, Chhourn Orn, 秋本正博, 宍戸理恵子, Than Myint Htun, Phuong Dang, Thai Phan, 野々村賢一, 小出陽平, 石川亮, 石井尊生

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    ポスター発表

  • ジャポニカ型栽培イネ(日本晴)の非脱粒性に関与する新規遺伝子座の離層形成への効果

    杉山昇平, 辻村雄紀, 大塚宏太郎, Than Myint Htun, 石井尊生, 石川亮

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    口頭発表(一般)

  • イネ育種における野生イネ利用の可能性

    石川亮, 宮崎直哉, 杉山昇平, 窪田芳生, 馬建鋒, 石井尊生

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    シンポジウム・ワークショップパネル(指名)

  • Exomeと果実・葉形状にみる日本のウメの多様性

    沼口孝司, 赤木剛士, 北村祐人, 大江孝明, 石川亮, 石井尊生

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    口頭発表(一般)

  • Evaluation of the domestication-related traits in rice: plant genetics meets archaeobotany

    Ryo Ishikawa

    International Workshop: Current Frontiers in the Archaeobotany of Rice, 2018年08月, 英語, Center for the Study of Chinese Archaeology, Peking University, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 野生イネOryza rufipogon における種子脱粒性遺伝子座間の相互作用

    杉山昇平, Cristina Castillo, 岡佑美, Than Myint Htun, 野々村賢一, 石井尊生, 石川亮

    イネ遺伝学・分子生物学ワークショップ2018, 2018年07月, 日本語, 国立遺伝学研究所, 国内会議

    ポスター発表

  • オーストラリア由来の野生イネOryza meridionalis を用いたイネ種子亜鉛濃度を支配する遺伝子座の同定

    宮崎直哉, 谷古健太, 岩田将英, 門田剛太郎, Orn Chhourn, 吉田周作, 石井尊生, 馬建鋒, 石川亮

    イネ遺伝学・分子生物学ワークショップ2018, 2018年07月, 日本語, 国立遺伝学研究所, 国内会議

    ポスター発表

  • イネにおける小穂の開花メカニズムの解明に向けて

    小川風和, 石川亮, 石井尊生

    近畿作物・育種研究会 第185回例会, 2018年06月, 日本語, 近畿作物・育種研究会, 神戸大学, 国内会議

    口頭発表(一般)

  • ジャポニカ型栽培イネOryza sativa 日本晴の非脱粒性に関与する新規遺伝子座の同定

    辻村雄紀, Than Myint Htun, 沼口孝司, 高間菜摘, 杉山昇平, 石井尊生, 石川亮

    第59回日本植物生理学会年会, 2018年03月, 日本語, 植物生理学会, 札幌コンベンションセンター, 国内会議

    ポスター発表

  • Evaluation of the domestication-related traits in rice: plant genetics meets archaeobotany

    Ryo Ishikawa

    New trends in Afro-Euraasian archaeobotany, 2018年01月, 英語, Nara Palace Site Museum (Nara National Research Institute for Cultural Properties), 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 野生イネの繁殖に必須の種子脱粒性に関わる遺伝子座の同定

    辻村雄紀, Than Myint Htun, 沼口孝司, 高間菜摘, 杉山昇平, 石井尊生, 石川亮

    第49回種生物学シンポジウム, 2017年12月, 日本語, 種生物学会, すかっとランド九頭竜, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonと栽培イネOryza sativaの交雑系統群を利用した新規Na+ 輸送系遺伝子・変異の探索

    堀内智貴, 石川亮, 田野井慶太朗, 小林奈通子, 石井尊生, 堀江智明

    日本育種学会第132回講演会, 2017年10月, 日本語, 日本育種学会, 岩手大学, 国内会議

    口頭発表(一般)

  • ウメ遺伝資源における収穫期および果実形質の多様性

    沼口孝司, 北村祐人, 仲慶晃, 大江孝明, 土田靖久, 石川亮, 石井尊生

    日本育種学会第132回講演会, 2017年10月, 日本語, 日本育種学会, 岩手大学, 国内会議

    口頭発表(一般)

  • embryoless変異体を用いたイネの胚・胚乳サイズ比率制御機構の解析

    金川真実, 松本和也, 石川亮, 伊藤純一, 桧原健一郎

    日本育種学会第132回講演会, 2017年10月, 日本語, 日本育種学会, 岩手大学, 国内会議

    口頭発表(一般)

  • ウメ黒星病抵抗性に関与するQTLの同定に向けて

    沼口孝司, 北村祐人, 石川亮, 石井尊生

    園芸学会 平成29年度秋季大会, 2017年09月, 日本語, 園芸学会, 酪農学園大学, 国内会議

    口頭発表(一般)

  • 栽培イネと野生イネの戻し交雑系統群を用いたイネの花器官を支配する遺伝子座の同定

    石川 亮, 渡部 貴史, 西岡 諒, Pham Thien Thanh, 石井 尊生

    日本育種学会第131回講演会, 2017年07月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • インディカ型栽培イネIR36の非脱粒性を支配する新規遺伝子座の探索

    辻村 雄紀, 井上 千鶴, Than Myint Htun, 西村 彬則, 杉山 昇平, 岡 佑美, 石井 尊生, 石川 亮

    日本育種学会第131回講演会, 2017年07月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • 野生イネ(Oryza rufipogon)の遺伝的背景で芒を短くするためには - イネの栽培化における無芒性の検証にむけて –

    池本 麻衣, 大塚 光晴, 石川 亮, 石井 尊生

    日本育種学会第131回講演会, 2017年03月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    口頭発表(一般)

  • 栽培イネと野生イネの戻し交雑系統群を用いたイネの花器官を支配する遺伝子座の同定

    石川 亮, 渡部 貴史, 西岡 諒, Pham Thien Thanh, 石井 尊生

    日本育種学会第131回講演会, 2017年03月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • シロイヌナズナのリン酸濃度応答遺伝子の探索

    鈴木 太郎, 大西 美輪, 菅野 里美, 手塚あゆみ, 出口 亜由美, 永野 惇, 石川 亮, 石崎 公庸, 深城 英弘, 三村 徹郎

    第58回日本植物生理学会年会, 2017年03月, 日本語, 日本植物生理学会, 鹿児島大学, 国内会議

    口頭発表(一般)

  • インディカ型栽培イネIR36の非脱粒性を支配する新規遺伝子座の探索

    辻村 雄紀, 井上 千鶴, Than Myint Htun, 西村 彬則, 杉山 昇平, 岡 佑美, 石井 尊生, 石川 亮

    日本育種学会第131回講演会, 2017年03月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogon由来の穂の開帳性に関する遺伝子座と関連遺伝子の発現について

    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, 石川 亮, 石井 尊生

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑自殖系統を用いた節からの出芽能力の評価

    池本 麻衣, 大八木 徹弥, Pham Thien Thanh, 石川 亮, 石井 尊生

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonとインディカ型栽培イネ(カサラス)の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, 石井 尊生, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    口頭発表(一般)

  • シロイヌナズナのリン酸濃度応答遺伝子の探索

    鈴木 太郎, 大西 美輪, 菅野 里美, 郷 達明, 豊倉 浩一, 永野 惇, 手塚 あゆみ, 石川 亮, 石崎 公庸, 深城 英弘, 三村 徹郎

    日本植物学会第80回大会, 2016年09月, 日本語, 日本植物学会, 沖縄コンベンションセンター, 国内会議

    ポスター発表

  • ジャポニカ型栽培イネ(日本晴)の非脱粒性に関与した遺伝子座の推定

    辻村 雄紀, Than Myint Htun, 沼口 孝司, 石井 尊生, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • オーストラリアの野生イネOryza meridionalisを用いた種子亜鉛濃度に関わる遺伝子座の同定とその効果の検証

    谷古 健太, 岩田 将英, 門田 剛太郎, Orn Chhourn, 吉田 周作, 石井 尊生, 馬, 建鋒, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    口頭発表(一般)

  • ウメ遺伝資源における黒星病およびかいよう病抵抗性の評価

    沼口 孝司, 北村 祐人, 土田 靖久, 仲 慶晃, 石川 亮, 石井 尊生

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • Oryza rufipogonとインディカ型栽培イネ(カサラス)の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, 石井 尊生, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    口頭発表(一般)

  • Evaluation of seed-shattering behaviour in early rice domestication

    Ryo Ishikawa, Yumi Oka, Yuki Tsujimura, Natsumi Takama, Chizuru Inoue, Than Myint Htun, Takashige Ishii

    8th World Archaeology Congress, 2016年09月, 英語, Doshisha University, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • A closed panicle played an important role in rice domestication

    Ryo Nishioka, Koji Numaguchi, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii

    8th World Archaeology Congress, 2016年09月, 英語, Doshisha University, 国際会議

    ポスター発表

  • 野生イネOryza rufipogon由来の穂の開帳性を支配する遺伝子座の推定と効果の検証

    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, 石川 亮, 石井 尊生

    イネ遺伝学・分子生物学ワークショップ, 2016年07月, 日本語, 名古屋大学, 国内会議

    ポスター発表

  • インディカ型栽培イネ(カサラス)の非脱粒性を支配するsh4座以外の遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, 辻村 雄紀, Than Myint Htun, 石井 尊生, 石川亮

    イネ遺伝学・分子生物学ワークショップ, 2016年07月, 日本語, 名古屋大学, 国内会議

    ポスター発表

  • Identification of quantitative trait loci controlling seed-shattering behaviour in rice

    Yuki Tsujimura, Than Myint Htun, Chizuru Inoue, Takashige Ishii, Ryo Ishikawa

    PAG Asia 2016, 2016年06月, 英語, Grand Copthorne Waterfront Hotel, 国際会議

    ポスター発表

  • Allelic interaction at awing loci in the genetic backgrounds of wild and cultivated rice

    Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii

    PAG Asia 2016, 2016年06月, 英語, Grand Copthorne Waterfront Hotel, 国際会議

    ポスター発表

  • 野生イネと栽培イネの間で検出されたアルミニウムストレス耐性を支配する遺伝子座qAl1の遺伝学的解析

    和田 もりえ, 岩田 将英, 横正 健剛, 吉田 周作, Pham Tien Thanh, 石井 尊生, 馬 建鋒, 石川 亮

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • 野生イネおよび栽培イネの遺伝的背景における芒の長さを支配する遺伝子座の対立遺伝子間の相互作用

    池本 麻衣, 大塚 光晴, 石川 亮, 石井 尊生

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑集団を用いた穂の開帳性に関するQTL解析

    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, 石川 亮, 石井 尊生

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • オーストラリア由来の野生イネOryza meridionalisを用いた種子亜鉛濃度に関わる遺伝子座の同定

    岩田 将英, 谷古 健太, 門田 剛太郎, Orn Chhourn, 吉田 周作, 石井 尊生, 馬 建鋒, 石川 亮

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • インディカ型栽培イネKasalathの脱粒性喪失に関与した新規遺伝子座の探索

    岡 佑美, 井上 千鶴, Than Myint Htun, 竹中 祥尭, 辻村 雄紀, 石井 尊生, 石川 亮

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • 野生イネ(Oryza rufipogon) の遺伝的背景における種子脱粒性と穂の開帳性の関係

    井上 千鶴, Than Myint Htun, 石井 尊生, 石川 亮

    日本育種学会第128回講演会, 2015年09月, 日本語, 日本育種学会, 新潟大学, 国内会議

    ポスター発表

  • Evaluation of the domestication-related traits in rice

    Ryo Ishikawa, Yumi Oka, Ryo Nishioka, Mai Ikemoto, Than Myint Htun, Chizuru Inoue, Koji Numaguchi, Takashige Ishii

    Early rice cultivation systems and their impact on social evolution and the environment., 2015年09月, 英語, University College London, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Evaluation of genetic variation among wild and cultivated rice in Cambodia

    Orn Chhourn, Rieko Shishido, Masahiro Akimoto, Ryo Ishikawa, Than Myint Htun, Ryo Nishioka, Men Sarom, Sakhan Sophany, Ouk Makara, Takashige. Ishii

    日本育種学会第128回講演会, 2015年09月, 英語, 日本育種学会, 新潟大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogon由来の穂の開帳性を支配する遺伝子座の推定

    西岡 諒, 三日月 裕美, Than Myint Htun, 石川 亮, 石井 尊生

    近畿作物・育種研究会 第179回例会, 2015年05月, 日本語, 近畿作物・育種研究会, 奈良県立橿原考古学研究所, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑自殖系統を用いた節からの出芽・出根能力およびひこばえの発生能力の評価

    池本 麻衣, 大八木 徹弥, Pham Thien Thanh, 石川 亮, 石井 尊生

    近畿作物・育種研究会 第179回例会, 2015年05月, 日本語, 近畿作物・育種研究会, 奈良県立橿原考古学研究所, 国内会議

    口頭発表(一般)

  • 野生イネと栽培イネのアルミニウムストレス耐性を支配する遺伝子座の同定とその効果

    和田 もりえ, 岩田 将英, 吉田 周作, Pham Thien Thanh, 石井 尊生, 馬 建鋒, 石川 亮

    日本育種学会第127回講演会, 2015年03月, 日本語, 日本育種学会, 玉川大学, 国内会議

    ポスター発表

  • インディカ型栽培イネ(Oryza sativa cv. IR36)における種子脱粒性の喪失に関与した遺伝子座数の推定

    西村 彬則, Than Myint Htun, 井上 千鶴, 岡 佑美, 石井 尊生, 石川 亮

    日本育種学会第127回講演会, 2015年03月, 日本語, 日本育種学会, 玉川大学, 国内会議

    ポスター発表

  • Evaluation of genetic variation among wild rice populations in Cambodia

    Orn Chhourn, Rieko Shishido, Hiroki Kageyama, Masahiro Akimoto, Ryo Ishikawa, Makara Ouk, Sarom Men, Takashige Ishii

    日本育種学会第127回講演会, 2015年03月, 英語, 日本育種学会, 玉川大学, 国内会議

    ポスター発表

  • Seed shattering behaviour and abscission layer formation in rice

    Cizuru Inoue, Than Myint Htun, Yumi Oka, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa

    Marchantia Workshop 2014, 2014年12月, 英語, Centennial Hall, Kobe University, 国際会議

    ポスター発表

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座の相互作用と離層形態

    井上 千鶴, Than Myint Htun, 井上 加奈子, 池田 健一, 石井 尊生, 石川 亮

    日本育種学会第126回講演会, 2014年09月, 日本語, 日本育種学会, 南九州大学, 国内会議

    ポスター発表

  • 野生イネと栽培イネのアルミニウムストレス耐性を支配する遺伝子座の同定

    和田 もりえ, 岩田 将英, 吉田 周作, Pham Thien Thanh, 石井 尊生, 馬 建鋒, 石川 亮

    イネ遺伝学・分子生物学ワークショップ2014, 2014年07月, 日本語, 東京大学, 国内会議

    シンポジウム・ワークショップパネル(公募)

  • 野生イネ(Oryza rufipogon)における種子脱粒性遺伝子座間の相互作用

    井上 千鶴, Than Myint Htun, Orn Chhourn, 岡 佑美, 石井 尊生, 石川 亮

    イネ遺伝学・分子生物学ワークショップ2014, 2014年07月, 日本語, 東京大学, 国内会議

    シンポジウム・ワークショップパネル(公募)

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用

    井上 千鶴, Than Myint Htun, 石井 尊生, 石川 亮

    日本育種学会第125回講演会, 2014年03月, 日本語, 日本育種学会, 東北大学, 国内会議

    口頭発表(一般)

  • 野生イネ(Oryza rufipogon)の遺伝的背景における栽培化関連形質の評価

    西村 彬則, 岩崎 友哉, 山本 知佳, 石川 亮, 石井 尊生

    日本育種学会第125回講演会, 2014年03月, 日本語, 日本育種学会, 東北大学, 国内会議

    口頭発表(一般)

  • Identification of a quantitative trait locus involved in abscission layer formation for seed shattering in Asian wild rice, Oryza rufipogon

    Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, Ryo Ishikawa

    日本育種学会第125回講演会, 2014年03月, 英語, 日本育種学会, 東北大学, 国内会議

    口頭発表(一般)

  • Evaluation of genetic diversity among rice varieties in Cambodia

    Orn Chhourn, Ryo Ishikawa, Sakhan Sophany, Ouk Makara, Takashige Ishii

    7th International Conference of Rice Genetics, 2013年11月, 英語, Dusit Thai Hotel Manila, Philippine, 国際会議

    ポスター発表

  • Closed panicles in rice domestication

    Ishii T, Numaguchi K, Miura K, Yoshida K, Thanh PT, Htun TM, Yamasaki M, Matsumoto T, Terauchi R, Ishikawa R, Ashikari M

    International Rice Genetics Symposium, 2013年11月, 英語, Manila, Philippines, 国際会議

    口頭発表(一般)

  • A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L.

    Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, Takashige Ishii

    7th International Rice Genetics Symposium, 2013年11月, 英語, Dusit Thai Hotel Manila, Philippine, 国際会議

    ポスター発表

  • A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L.

    Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, Takashige Ishii

    7th International Conference of Rice Genetics, 2013年11月, 英語, Dusit Thai Hotel Manila, Philippine, 国際会議

    ポスター発表

  • 穂が閉じることによって野生イネはどう変化したか?

    沼口 孝司, Htun Than Myint, Thanh Pham Thien, 石川 亮, 石井 尊生

    日本育種学会第124回講演会, 2013年10月, 日本語, 日本育種学会, 鹿児島大学, 国内会議

    口頭発表(一般)

  • イネの胚、胚乳サイズ比率に関わるEMBRYOLESS1遺伝子の解析

    堀島 佑月, 琴 梨世, 石川 亮, 桧原 健一郎, 伊藤 純一, 長戸 康郎

    日本育種学会第124回講演会, 2013年10月, 日本語, 日本育種学会, 鹿児島大学, 国内会議

    口頭発表(一般)

  • Wild QTLs analysis for agronomic traits using recombinant inbred lines between Oryza rufipogon and O. sativa

    Pham Thien Thanh, Phuong Dang, Thai Phan, 石川 亮, 石井 尊生

    日本育種学会第124回講演会, 2013年10月, 英語, 日本育種学会, 鹿児島大学, 国内会議

    ポスター発表

  • 野生イネの遺伝的背景における栽培イネの非脱粒性遺伝子の効果

    石川 亮, Than Myint Htun, 山崎 将紀, Pham Thien Thanh, 石井 尊生

    第52回日本植物生理学会年会, 2011年03月, 日本語, 日本植物生理学会, 東北大学, 国内会議

    口頭発表(一般)

  • 野生イネ (Oryza rufipogon)の種子有芒性の評価および遺伝子マッピング

    大塚 光晴, 石川 亮, 石井 尊生

    日本育種学会第119回講演会, 2011年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    口頭発表(一般)

  • 栽培イネ Oryza sativa Nipponbareを野生イネO. rufipogon W630で戻し交雑した自殖系統群を用いた植物と種子の形態形質に関するQTL解析

    山本 知佳, 岩崎 友哉, 石川 亮, 石井 尊生

    日本育種学会第119回講演会, 2011年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    口頭発表(一般)

  • フィトクロムによるHd1を介したフロリゲン遺伝子Hd3aの転写制御と日長認識機構

    石川 亮, 青木 麻由美, 黒谷 賢一, 横井 修司, 篠村 知子, 高野 誠, 島本 功

    日本育種学会第119回講演会, 2011年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    口頭発表(一般)

  • イネ無胚乳変異原因遺伝子ENL1はSNF2ヘリカーゼ様タンパク質をコードする

    加藤 大和, 原 睦美, 佐藤 豊, 北野 英己, 長戸 康郎, 石川 亮, 木下 哲, 武田 真, 服部 束穂

    第52回日本植物生理学会年会, 2011年03月, 日本語, 日本植物生理学会, 東北大学, 国内会議

    口頭発表(一般)

  • イネ属種間交雑における胚乳発生異常とOsMADS87インプリント遺伝子の制御異常

    大西 孝幸, 石川 亮, 木下 由紀, 永口 貢, 倉田 のり, 木下 哲

    第33回日本分子生物学会年会・第83回日本生化学会大会合同大会, 2010年12月, 日本語, 日本分子生物学会, 神戸ポートアイランド, 国内会議

    シンポジウム・ワークショップパネル(公募)

  • イネSNF2 ヘリカーゼ様タンパク質をコードするENDOSPERMLESS1(ENL1)の欠損は胚乳核の分裂異常を示す

    原 睦美, 加藤 大和, 佐藤 豊, 北野 英己, 長戸 康郎, 石川 亮, 木下 哲, 武田 真, 服部 束穂

    第33回日本分子生物学会年会・第83回日本生化学会大会合同大会, 2010年12月, 日本語, 日本分子生物学会, 神戸ポートアイランド, 国内会議

    ポスター発表

  • 野生イネと私:野生種を用いた新たな発見とその育種利用に向けて

    石川 亮

    日本育種学会第118回講演会, 2010年09月, 日本語, 日本育種学会, 秋田県立大学, 国内会議

    口頭発表(招待・特別)

  • 高等植物胚乳における種の障壁成立のルールとメカニズム

    木下 哲, 大西 孝幸, 石川 亮, 木下 由紀, 永口 貢, 倉田のり

    日本遺伝学会第82回大会, 2010年09月, 日本語, 日本遺伝学会, 札幌市, 国内会議

    シンポジウム・ワークショップパネル(公募)

  • イネのbiotoron breeding system; 年6度の戻し交配を可能にする栽培法

    大西 孝幸, 石川 亮, 山川 博幹, 木下 哲

    日本育種学会第118回講演会, 2010年09月, 日本語, 日本育種学会, 秋田県立大学, 国内会議

    口頭発表(一般)

  • QTL analysis for spikelet opening time using backcross inbred lines between Oryza sativa Nipponbare and O. rufipogon

    Pham Thien Thanh, Phan Dang, Thai Phuong, Ryo Ishikawa, Takashige Ishii

    日本育種学会第117回講演会, 2010年03月, 英語, 日本育種学会, 京都大学, 国内会議

    口頭発表(一般)

  • Estimation of outcrossing rate of Asian wild rice, Oryza rufipogon, under field condition

    Phan Dang Thai Phuong, Hiroki Kageyama, Ryo Ishikawa, Takashige Ishii

    日本育種学会第117回講演会, 2010年03月, 英語, 日本育種学会, 京都大学, 国内会議

    口頭発表(一般)

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

    Pham Thien Thanh, Ryo Ishikawa, Naoki Mori, Takashige Ishii

    6th International Conference of Rice Genetics, 2009年11月, 英語, Manila Hotel, Philippine, 国際会議

    ポスター発表

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

    Thanh PT, Ishikawa R, Mori N, Ishii T

    International Rice Genetics Symposium, 2009年11月, 英語, Manila, Philippines, 国際会議

    ポスター発表

  • Genomic imprinting and reproductive barrier observed in the hybrid endosperm of rice

    Ryo Ishikawa, Mitsugu Eiguchi, Yuki Shinzaki, Nori Kurata, Tetsu Kinoshita

    6th International Conference of Rice Genetics, 2009年11月, 英語, Manila Hotel, Philippine, 国際会議

    ポスター発表

  • Abnormal development observed in the hybrid endosperm between cultivated and wild rice

    Ryo Ishikawa, Mitsugu Eiguchi, Yuki Shinzaki, Nori Kurata, Tetsu Kinoshita

    6th International Conference of Rice Genetics, 2009年11月, 英語, Manila Hotel, Philippine, 国際会議

    ポスター発表

  • 栽培イネと野生イネの種間交雑にみられる胚乳発生異常の原因

    石川 亮, 永口 貢, 新崎 由紀, 倉田 のり, 木下 哲

    第50回日本植物生理学会年会, 2009年03月, 日本語, 日本植物生理学会, 名古屋大学, 国内会議

    口頭発表(一般)

  • ゲノムインプリンティングを介した生殖隔離

    石川 亮, 木下哲

    第40回種生物学シンポジウム, 2008年12月, 日本語, 種生物学会, 守谷市 デュープレックスセミナーホテル, 国内会議

    口頭発表(招待・特別)

  • Genomic imprinting and reproductive barrier observed in the hybrid endosperm of rice

    Ryo Ishikawa, Mitsugu Eiguchi, Nori Kurata, Tetsu Kinoshita

    EMBO World Workshop Genomic Imprinting, 2008年09月, 英語, Temasek LifeSciences Laboratory, Singapore, 国際会議

    ポスター発表

  • イネの胚乳における生殖隔離機構とゲノムインプリンティング

    石川 亮, 永口 貢, 池田 陽子, 倉田 のり, 木下 哲

    第49回日本植物生理学会年会, 2008年03月, 日本語, 日本植物生理学会, 北海道札幌コンベンションセンター, 国内会議

    口頭発表(一般)

  • イネの開花におけるフィトクロムの役割

    石川 亮, 青木 麻由美, 黒谷 賢一, 清水 久代, 横井 修司, 稲垣 言要, 篠村 知子, 高野 誠, 島本 功

    第48回日本植物生理学会年会, 2007年03月, 日本語, 日本植物生理学会, 愛媛大学, 国内会議

    口頭発表(一般)

  • Hd1過剰発現植物を用いたイネ開花経路の解析

    青木 麻由美, 黒谷 賢一, 石川 亮, 横井 修司, 島本 功

    第29回日本分子生物学会, 2006年12月, 日本語, 日本分子生物学会, 京都, 国内会議

    ポスター発表

  • イネの開花におけるフィトクロムの作用

    石川 亮, 篠村 知子, 高野 誠, 島本 功

    日本育種学会第110回講演会, 2006年09月, 日本語, 日本育種学会, 愛媛大学, 国内会議

    口頭発表(一般)

  • Natural variation in flowering time genes and night-break effects in rice

    Yasuyuki Takahashi, Ryo Ishikawa, Shuji Yokoi, Ko Shimamoto

    8th International Congress on Plant Molecular Biology, 2006年08月, 英語, Adelaide, South Australia, 国際会議

    ポスター発表

  • Natural variation in flowering time genes and night-break effects in rice

    Yasuyuki Takahashi, Ryo Ishikawa, Shuji Yokoi, Ko Shimamoto

    20th IUPMB International Congress of Biochemistry and Molecular Biology, 2006年06月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Hd1 overexpression delays flowering in rice under SD conditions

    Mayumi Aoki, Ken-ichi Kurotani, Ryo Ishikawa, Shuji Yokoi, Ko Shimamoto

    20th IUPMB International Congress of Biochemistry and Molecular Biology, 2006年06月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Effects of light quality on night-break in rice flowering

    Ryo Ishikawa, Tomoko Shinomura, Takanari Tanabata, Hisayo Shimizu, Noritoshi Inagaki, Makoto Takano, Ko Shimamoto

    20th IUPMB International Congress of Biochemistry and Molecular Biology, 2006年06月, 英語, Kyoto, Japan, 国際会議

    ポスター発表

  • Effects of light quality on night-break in rice flowering

    Ryo Ishikawa, Shojiro Tamaki, Yasuyuki Takahashi, Toru Ishizuka, Shuji Yokoi, Noritoshi Inagaki, Tomoko Shinomura, Makoto Takano, Ko Shimamoto

    International Plant Photobiology Meeting, 2006年04月, 英語, Paris, France, 国際会議

    ポスター発表

  • イネの光中断おける光質とフィトクロムの役割

    石川 亮, 篠村 知子, 七夕 高也, 清水 久代, 稲垣 言要, 高野 誠, 島本 功

    第47回日本植物生理学会年会, 2006年03月, 日本語, 日本植物生理学会, 筑波大学, 国内会議

    ポスター発表

  • イネコアコレクションを用いた開花関連遺伝子の多様性の解明

    高橋 靖幸, 石川 亮, 横井 修司, 島本 功

    第47回日本植物生理学会年会, 2006年03月, 日本語, 日本植物生理学会, 筑波大学, 国内会議

    ポスター発表

  • Molecular basis of night break effect on rice flowering

    Ryo Ishikawa, Shojiro Tamaki, Yasuyuki Takahashi, Toru Ishizuka, Shuji Yokoi, Noritoshi Inagaki, Tomoko Shinomura, Makoto Takano, Ko Shimamoto

    5th International Rice Genetics Symposium, 2005年11月, 英語, Manila, Philippines, 国際会議

    ポスター発表

  • イネの開花における光中断の分子遺伝学的解析

    石川 亮, 玉置 祥二郎, 高橋 靖幸, 石塚 徹, 横井 修司, 稲垣 言要, 篠村 知子, 高野 誠, 島本 功

    第77回日本遺伝学会年会, 2005年09月, 日本語, 日本遺伝学会, 国立オリンピック記念青少年総合センター, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Molecular basis of night break effect on flowering in rice

    Ryo Ishikawa, Takahashi Yasuyuki, Toru Ishizuka, Shuji Yokoi, Noritoshi Inagaki, Tomoko Shinomura, Makoto Takano, Ko Shimamoto

    日本育種学会第107,108回講演会, 2005年08月, 英語, 日本育種学会, 筑波大学, 国内会議

    ポスター発表

  • Molecular basis of night break effect on flowering in rice

    Ryo Ishikawa, Yasuyuki Takahashi, Toru Ishizuka, Shuji Yokoi, Noritoshi Inagaki, Tomoko Shinomura, Makoto Takano, Ko Shimamoto

    10th International Congress of SABRAO, 2005年08月, 英語, Tsukuba International Congress Centre, 国際会議

    ポスター発表

  • 野生イネ由来の種子脱粒性、穂の開帳性、種子色を支配する遺伝子のマッピング

    石井 尊生, 崎山 弘樹, 石川 亮, 上島 脩志

    近畿作物育種研究会, 2005年07月, 日本語, 近畿作物育種研究会, 神戸大学, 国内会議

    口頭発表(一般)

  • イネにおける光中断効果は開花促進因子Hd3aの発現抑制による

    石川 亮, 玉置 祥二郎, 高橋 靖幸, 石塚 徹, 横井 修司, 稲垣 言要, 篠村 知子, 高野 誠, 島本 功

    イネ・ナズナ合同ワークショップ, 2005年07月, 日本語, 奈良県文化会館, 国内会議

    口頭発表(一般)

  • 分子遺伝学的な方法論での探索—短日植物イネでの研究例

    島本 功, 玉置 祥二郎, 小宮 怜奈, 松尾 祥一, 石川 亮, 池田 美香, 阿部 誠, 黒谷 賢一, 横井 修司

    第46回日本植物生理学会年会, 2005年03月, 日本語, 日本植物生理学会, 新潟朱鷺メッセ, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • イネの光中断おけるフィトクロムの役割

    石川 亮, 高橋 靖幸, 石塚 徹, 横井 修司, 稲垣 言要, 篠村 知子, 高野 誠, 島本 功

    第46回日本植物生理学会年会, 2005年03月, 日本語, 日本植物生理学会, 新潟朱鷺メッセ, 国内会議

    口頭発表(一般)

  • イネにおける開花制御の遺伝子ネットワーク

    島本 功, 玉置 祥二郎, 石川 亮, 阿部 誠, 小宮 怜奈, 池田 美香, 松尾 祥一, 黒谷 賢一, 横井 修司

    公開シンポジウム「植物の生殖研究-その最前線と今後の方向」, 2004年11月, 日本語, 東京大学, 国内会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • Molecular basis of night break effect on flowering in rice

    Ryo Ishikawa, Shuji Yokoi, Ko Shimamoto

    World Rice Research Conference, 2004年11月, 英語, Epochal Tsukuba, Tsukuba, Japan, 国際会議

    ポスター発表

  • 栽培イネ(Oryza sativa Nipponbare)の遺伝背景における野生イネ(Oryza rufipogon)由来の脱粒性遺伝子の作用について

    二村 直人, 石川 亮, 上島 脩志, 石井 尊生

    日本育種学会第106回講演会, 2004年09月, 日本語, 日本育種学会, 三重大学, 国内会議

    口頭発表(一般)

  • イネの開花を制御する遺伝子ネットワーク

    島本 功, 玉置 祥二郎, 石川 亮, 阿部 誠, 小宮 怜奈, 池田 美香, 松尾 祥一, 黒谷 賢一, 横井 修司

    第76回日本遺伝学会年会, 2004年09月, 日本語, 日本遺伝学会, 大阪大学コンベンションセンター, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • Molecular basis of night break effect on flowering in rice

    Ryo Ishikawa, Shojiro Tamaki, Shuji Yokoi, Ko Shimamoto

    58th Yamada Conference on Light Sensing and Signal Transduction in Plant Photomorphogenesis, 2004年06月, 英語, Okazaki Conference Center, Okazaki, Japan, 国際会議

    ポスター発表

  • 植物はいかに日長に応じて花を咲かせるのか?

    島本 功, 玉置 祥二郎, 石川 亮, 阿部 誠, 小宮 怜奈, 池田 美香, 松尾 祥一, 黒谷 賢一, 横井 修司

    日本分子生物学会(奈良春大会), 2004年05月, 日本語, 日本分子生物学会, 奈良県, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • 光中断を利用したイネの開花誘導における遺伝子発現の解析

    石川 亮, 横井 修司, 島本 功

    日本分子生物学会(奈良春大会), 2004年05月, 日本語, 日本分子生物学会, 奈良県, 国内会議

    ポスター発表

  • Molecular basis of night break effect on flowering in rice

    Ryo Ishikawa, Shuji Yokoi, Ko Shimamoto

    Crop Functional Genomics, 2004年04月, 英語, Ramada Plaza Hotel, Jeju Island, Korea, 国際会議

    ポスター発表

  • 光中断を利用したイネの開花誘導における遺伝子発現の解析

    石川 亮, 横井 修司, 島本 功

    第45回日本植物生理学会年会, 2004年03月, 日本語, 日本植物生理学会, 東京都立大学, 国内会議

    口頭発表(一般)

  • イネの日長による開花制御の分子機構 Ⅱ、光中断の効果とその分子機構

    石川 亮, 横井 修司, 島本 功

    日本育種学会第105回講演会, 2004年03月, 日本語, 日本育種学会, 東京大学, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogon由来の脱粒性遺伝子のマッピング

    二村 直人, 石川 亮, 上島 脩志, 石井 尊生

    日本育種学会第104回講演会, 2003年09月, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 植物の開花における短日・長日性はどうして決められるか

    島本 功, 玉置 祥二郎, 石川 亮, 阿部 誠, 池上 顕子, 横井 修司

    日本発生生物学会第36回大会, 2003年06月, 日本語, 日本発生生物学会, 札幌コンベンションセンター, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • Hd3a, an ortholog of FT, is a target of night break in the photoperiodic control of flowering in rice

    Ryo Ishikawa, Shuji Yokoi, Ko Shimamoto

    7th International Congress of Plant Molecular Biology, Barcelona, Spain, 2003年06月, 英語, 国際会議

    ポスター発表

  • イネの短日性を決定する遺伝子ネットワーク

    島本 功, 石川 亮, 阿部 誠, 玉置 祥二郎, 早間 良輔, 井澤 毅, 横井 修司

    第44回日本植物生理学会年会, 2003年03月, 日本語, 日本植物生理学会, 近畿大学, 国内会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • イネ野生種(Oryza rufipogon)由来の脱粒性遺伝子のマッピング

    石川 亮, 上島 脩志, 石井 尊生

    日本育種学会第101回講演会, 2002年03月, 日本語, 日本育種学会, 玉川大学, 国内会議

    口頭発表(一般)

所属学協会

  • 日本育種学会

共同研究・競争的資金等の研究課題