研究者紹介システム

水野 雅史
ミズノ マサシ
大学院農学研究科 生命機能科学専攻
教授
農芸化学関係
Last Updated :2021/04/12

研究者情報

所属

  • 【主配置】

    大学院農学研究科 生命機能科学専攻
  • 【配置】

    農学部 生命機能科学科

学位

  • 学術博士, 神戸大学

研究シーズ

授業科目

ジャンル

  • 科学・技術 / 生命科学

コメントテーマ

  • 免疫賦活物質
  • キノコ
  • 活性酸素
  • 抗腫瘍性
  • 腸管免疫

研究活動

研究キーワード

  • 腸管免疫
  • 抗腫瘍性
  • 活性酸素
  • 高分子多糖
  • 免疫賦活

研究分野

  • ライフサイエンス / 食品科学

委員歴

  • 日本農芸化学会, 関西支部評議委員

受賞

  • 2015年10月 公益社団法人日本生物工学会, 生物工学技術賞, 生酛乳酸菌のバイオジェニック効果に着目した米乳酸発酵飲料の開発

    水野 雅史

    日本国

    国内学会・会議・シンポジウム等の賞

  • 2009年07月 森喜作記念椎茸振興会, 森喜作賞, キノコの医学的研究

    水野 雅史

  • 2001年09月 社団法人 日本食品科学工学会, 日本食品科学工学会奨励賞, 機能性多糖の免疫学的測定法の確立とその応用

論文

  • Oral administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin

    SAKIKO OKA, NOBUAKI OHTO, HIROSHIGE KUWAHARA, MASASHI MIZUNO

    2020年02月, European Food Research and Technology, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Oral administration of fucoidan can exert anti-allergic activity after allergen sensitization by enhancement of galectin-9 secretion in blood

    MASASHI MIZUNO, KANA SAKAGUCHI, IWAO SAKANE

    2020年02月, Biomolecules, 10 (2), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Influence of temperature on anti-allergic activity of fucoidan extracted from Saccharina japonica

    MORITA ATSUKO, TANINO YUKA, OJIMA TAKAO, MIZUNO MASASHI

    日本食品科学工学会, 2019年07月, Food Science and Technology Research, 25 (4), 607 - 611, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation

    MINATO KEN-ICHIRO, MIZUNO MASASHI

    Elsevier, 2019年01月, International Journal of Biological Macromolecules, 122, 705 - 712, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 褐藻類由来高分子多糖フコイダンによる新規抗アレルギー機構

    水野 雅史, 湊 健一郎

    2018年12月, Food Processing and Ingredients, 53 (12), 7 - 9, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Lentinan Exerts its Anti-Inflammatory Activity by Suppressing TNFR1 Transfer to the Surface of Intestinal Epithelial Cells through Dectin-1 in an in vitro and mice model

    SAKAGUCHI KANA, SHIRAI YASUHITO, ITOH TOSHIKI, MIZUNO MASASHI

    2018年12月, Immunome Research, (14), 165, 英語

    [査読有り][招待有り]

    研究論文(学術雑誌)

  • Masashi Mizuno, Iwao Sakane, Ken-ichiro Minato, Junya Watanabe, Takashi Hashimoto

    The anti-inflammatory activity of Grifola gargal was evaluated using an in vitro gut inflammation model composed of RAW264.7 and Caco-2 cells. The active compound was isolated and purified to elucidate its structure by instrumental analysis. A low-molecular-weight fraction in G. gargal prepared using an ultrafiltration membrane (13 000 Da molecular-weight cut-off) suppressed tumor necrosis factor-alpha production and interleukin-8 mRNA expression in an in vitro gut inflammation model. The low-molecular-weight fraction was subjected to sequential extraction, consisting of reverse-phase extraction using 0-100% ethanol, in 20% steps, and high-performance liquid chromatography to separate and purify the active compound with anti-inflammatory activity. Instrument analyses indicated that adenine was one of the active compounds. It was suggested that adenine in G. gargal had no small effect on the suppression of inflammatory bowel disease.

    KARGER, 2017年09月, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 23 (5), 725 - 732, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mendel Friedman, Nobuyuki Kozukue, Hyun-Jeong Kim, Suk-Hyun Choi, Masashi Mizuno

    Potato peels from the large-scale peeling of potatoes are a source of bioactive compounds. We determined the content of the glycoalkaloids alpha-chaconine and alpha-solanine, individual and total phenolics compounds, and total flavonoids extracted from three peels prepared from conventional and three from organic commercial gold, red, and Russet potatoes as well as determining their antioxidant activities using three different methods. Glycoalkaloids and individual phenolic compounds (caffeic acid, chlorogenic acid, and chlorogenic acid isomer) were determined using HPLC and LC MS, and total phenolic and flavonoid content by colorimetry. There was a wide variation in the content of total glycoalkaloids, phenolic compounds, flavonoids, and antioxidative activities, as indicated by the following ratios for the total organic to nonorganic glycoalkaloids: gold, 3.89; Russet, 2.43; and red, 0.50. The corresponding phenolics and flavonoid ratios indicated a narrower range. The described methods could be used to determine the composition and antioxidative activities of peels derived from other potato cultivars, taking into account their methods of growth and postharvest storage, and aid in compositional, nutritional, medical, and industrial studies on potato peel, to relate their composition to their many described applications.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017年09月, JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 62, 69 - 75, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashi Kuwata, Takashi Hashimoto, Nobuaki Ohto, Hiroshige Kuwahara, Jae Won Lee, Takeshi Bamba, Masashi Mizuno

    Glucosylceramide (G1cCer) has various effects such as skin moisturizing, improvement of atopic dermatitis, and enhancement of skin barrier function. However, the mechanism of GlcCer on skin barrier function remains unclear. In this study, the effect of G1cCer from pineapples (P-GlcCer) on dry skin protection and intestinal immunity was investigated using HR-1 hairless mice fed on a special diet and an in vitro co-culture model consisting of intestinal epithelial Caco-2 and macrophage RAW264.7. The oral administration of P-GlcCer showed an improvement in skin barrier function and enhancement of interleukin-23p19 mRNA (I123a) expression in the small intestine. 1123a expression in LPS-stimulated RAW264.7 cells was enhanced by the purified GlcCer in P-GlcCer. Furthermore, pretreatment of Caco-2 with a beta-glucocere brosid ase or a ceramidase inhibitor suppressed the enhancement of112.3a expression, but a sphingosine kinase inhibitor did not. It was demonstrated that not P-GIcCer itself but the intestinal metabolite 4,8-sphingadienine ameliorated dry skin symptoms. (C) 2017 Elsevier Ltd. All rights reserved.

    ELSEVIER SCIENCE BV, 2017年03月, JOURNAL OF FUNCTIONAL FOODS, 30, 228 - 236, 英語

    [査読有り]

    研究論文(学術雑誌)

  • スマート乳酸菌の新機能

    大戸 信明, 水野 雅史

    2017年, Food Style21, 21 (3), 59 - 62, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Enhancement of anti-inflammatory and anti-allergic activities with combination of luteolin and quercetin in in vitro co-culture system

    MIZUNO MASASHI, YAMASHITA SAE, HASHIMOTO TAKASHI

    2017年, Food Science and Technology Research, 23 (6), 811 - 818, 英語

    [査読有り]

    研究論文(学術雑誌)

  • An in vitro investigation of immunomodulatory properties of Lactobacillus plantarum and L. delbrueckii cells and their extracellular polysaccharides

    KISHIMOTO MANA, NOMOTO RYOHEI, MIZUNO MASASHI, OSAWA RO

    2017年, Bioscience of. Microbiota, Food and Health, 36 (3), 101 - 110, 英語

    [査読有り]

    研究論文(学術雑誌)

  • MINATO KEN-ICHIRO, OHARA AKIHIRO, MIZUNO MASASHI

    PCPS from P. citrinopileatus mushroom extract is a beta-1,6-glucan possessing a proinflammatory effect on innate immune cells. The PCPS stimulated THP-1 macrophages to secrete significant levels of TNF. Moreover, the mRNA expressions of TNF and IL-1 beta were significantly enhanced by PCPS treatment. However, the PCPS did not induce to express both IL-12 and IL-10 mRNA in the macrophages. Next, the P. cornucopiae extract (containing mainly PCPS) treatment against mice showed significant increases in TNF and IL-1 beta mRNA expressions in the peritoneal macrophages of them. In this study, the expression levels of IFN gamma mRNA in the spleen were almost the same between the extract- (PCPS-) treated group and control group. However, the expression of IL-4 mRNA showed a lower level in the extract-treated group than that in the control. Our results suggested that the PCPS could induce proinflammatory action in the immune response. In addition, the proinflammatory effect of the PCPS on THP-1 was enhanced by 5'-GMP-Na, while it was reduced by vitamin D-2. These two compounds are majorly contained in the P. citrinopileatus mushroom. Therefore, these results suggested that the P. citrinopileatus mushroom might contain other immune regulative compounds, such as vitamin D-2, as well as PCPS.

    Hindawi, 2017年, Mediators of inflammation, 8402405, 9, 英語

    [査読有り][招待有り]

    研究論文(学術雑誌)

  • Yuka Tanino, Takashi Hashimoto, Takao Ojima, Masashi Mizuno

    Fucoidan is a sulfated polysaccharide from brown sea algae. In the present study, it was demonstrated that oral administration of F-fucoidan from Saccharina japonica possessed anti-allergic effects using the passive cutaneous anaphylaxis reaction, but not by intraperitoneal administration. The inhibitory mechanism was dependent on galectin-9, which belongs to a soluble lectin family that recognizes p-galactoside and prevents IgE binding to mast cells. The anti-allergy properties of F-fucoidan were cancelled by an intravenous dose of anti-galectin-9 antibody or lactose, which bind competitively with galectin-9 before the passive cutaneous anaphylaxis reaction. F-fucoidan increased the expression level of galectin-9 mRNA in intestinal epithelial cells and serum galectin-9 levels. Oral treatment with F-fucoidan suppressed allergic symptoms through the induction of galectin-9. This is the first report that F-fucoidan can induce the secretion of galectin-9.

    JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, 2016年07月, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 59 (1), 25 - 30, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kazuki Kanazawa, Miku Sakamoto, Ko Kanazawa, Yoriko Ishigaki, Yoshiko Aihara, Takashi Hashimoto, Masashi Mizuno

    The oxidation of guanosine to 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA is closely associated with induction of various diseases, but the endogenous oxidant species involved remains unclear. Hydrogen peroxides (H2O2) have been considered to be the oxidant, while lipid peroxides are another possible oxidant because generated easily in bio-membranes surrounding DNA. The oxidant potency was compared between lipid peroxides and H2O2. Linoleic acid hydroperoxides (LOOH) formed 8-oxo-dG at a higher level than H2O2 in guanosine or double-stranded DNA. In the presence of a physiological concentration of Fe2+ to produce hydroxyl radicals, LOOH was also a stronger oxidant. In a lipid micelle, LOOH markedly produced 8-oxo-dG at a concentration one-tenth of that of H2O2. Upon adding to rat hepatic mitochondria, phosphatidylcholine hydroperoxides produced 8-oxo-dG abundantly. Employing HepG2 cells after pretreated with glutathione peroxidase inhibitor, LOOH formed 8-oxo-dG more abundantly than H2O2. Then, antioxidants to suppress the 8-oxo-dG formation were examined, when the nuclei of pre-incubated HepG2 with antioxidants were exposed to LOOH. Water-soluble ascorbic acid, trolox, and N-acetyl cysteine showed no or weak antioxidant potency, while lipid-soluble 2,6-dipalmitoyl ascorbic acid, alpha-tocopherol, and lipid soluble phytochemicals exhibited stronger potency. The present study shows preferential formation of 8-oxo-dG upon LOOH and the inhibition by lipid-soluble antioxidants.

    JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, 2016年07月, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 59 (1), 16 - 24, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A novel in vitro co-culture model comprised of Caco-2/RBL-2H3 cells to evaluate anti-allergic effects of food factors through the intestine

    YAMASHITA SAE, YOKOYAMA YUKI, HASHIMOTO TAKASHI, MIZUNO MASASHI

    Elsevier, 2016年04月, Journal of Immunological Method, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 生酛乳酸菌のバイオジェニック効果に着目した米乳酸発酵飲料の開発

    高橋 俊成, 増田 康之, 吉田 和利, 水野 雅史

    マクロファージ様細胞株、マウス、培養細胞の共培養系を利用した腸管免疫モデルなどを用いて免疫調節作用の高い乳酸菌としてLactobacillus sakei LK-117株を選抜した。この乳酸菌体をアトピー性皮膚炎を発症するマウスに継続投与したところ、アトピー性皮膚炎の炎症が抑制された。

    日本生物工学会, 2016年02月, 生物工学会誌, 94 (2), 63 - 69, 日本語

    [査読有り][招待有り]

    研究論文(学術雑誌)

  • Won-Young Song, Yoshiko Aihara, Takashi Hashimoto, Kazuki Kanazawa, Masashi Mizuno

    The anorexigenic gut hormones, cholecystokinin (CCK), glucagonlike peptide (GLP)-1 and peptide tyrosine-tyrosine (PYY), are released in response to food intake from the intestines. Dietary nutrients have been shown to stimulate these hormones. Some non-nutrients such as polyphenols show anorexigenic effects on humans. In the present study, we examined whether dietary polyphenols can stimulate secretion of these gut hormones. Caco-2 cells expressed mRNA of the gut hormones, CCK, PC1 (prohormone convertase 1), GCG (glucagon) and PYY. CCK, GLP-1 and PYY were secreted from Caco-2 cells after adding sugars, amino acids or fatty acids. Using Caco-2 cells, epigallocatechin-3-gallate (EGCG), chlorogenic acid and ferulic acid induced secretion of anorexigenic gut hormones. Particularly, EGCG induced secretion of all three hormones. In an ex vivo assay using murine intestines, EGCG also released CCK from the duodenum, and GLP-1 from the ileum. These results suggest that EGCG may affect appetite via gut hormones.

    JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, 2015年09月, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 57 (2), 164 - 169, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naotake Konno, Keiko Nakade, Yosuke Nishitani, Masashi Mizuno, Yuichi Sakamoto

    Lentinan from Lentinula edodes fruiting bodies (shiitake mushrooms) is a valuable beta-glucan for medical purposes based on its anticancer activity and immunomodulating activity. However, lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity. In this study, we downregulated the expression of an exo-beta-1,3-glucanase, exg2, in L. edodes using RNA interference. In the wild-type strain, beta-1,3-glucanase activity in fruiting bodies remarkably increased after harvesting, and 41.7% of the lentinan content was lost after 4 days of preservation. The EXG2 downregulated strain showed significantly lower lentinan degrading activity (60-70% of the wild-type strain) in the fruiting bodies 2-4 days after harvesting. The lentinan content of fresh fruiting bodies was similar in the wild-type and EXG2 downregulated strains, but in the downregulated strain, only 25.4% of the lentinan was lost after 4 days, indicating that downregulation of EXG2 enables keeping the lentinan content high longer.

    ACS Publicatoins, 2014年08月, Journal of Agricutural and Food Chemistry, 62 (32), 8153 - 8157, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Suk Hyun Choi, Dong-Seok Kim, Nobuyuki Kozukue, Hyun-Jeong Kim, Yosuke Nishitani, Masashi Mizuno, Carol E. Levin, Mendel Friedman

    The content of water, free amino acids, amino acid metabolites, crude protein, the carotene pigments beta-carotene and lycopene, and 9 characterized and 2 incompletely characterized individual phenolic (flavonoid) compounds of 12 greenhouse-grown cherry tomato varieties of various colors (green, yellow, orange, red, and black) was determined using HPLC and LC/MS methods. The phenolic content of the cherry tomatoes per unit weight is 3-4 times greater than reported values for large-sized tomatoes. Antioxidative effects using the ABTS and FRAP assays and cancer-cell-inhibiting effects against 2 normal (Chang liver and He1299 lung) and 3 human cancer (lung A549; liver HepG2; and cervical HeLa) cell lines using the MTT cell viability assay were also determined. Lycopene inhibited all the cell lines, but showed strong activity against the cervical carcinoma and the lung cancer cells. The tomato extracts showed inhibition at the higher doses. The HeLa cervical carcinoma cell line was most inhibited by the pure compounds, and the HeLa or the HepG2 cells lines were the most inhibited by the tomato extracts. The results demonstrate wide-ranging differences as well as similarities in the content of nutritional and bioactive compounds in cherry tomatoes, and suggest that such knowledge can benefit consumers. Published by Elsevier Inc.

    Journal of Food Composition and Anaylysis, 2014年06月, Protein, free amino acid, phenolic, beta-carotene, and lycopene content, and antioxidative and cancer cell inhibitory effects of 12 greenhouse-grown commercial cherry tomato varieties, 34 (2), 115 - 127, 英語

    [査読有り]

    研究論文(学術雑誌)

  • スマート乳酸菌(Lactobacillus plantarum 22A-3)におけるポリフェノール吸収促進およびプロバイオティックス効果について

    林 多恵子, 大澤 朗, 水野 雅史

    2014年, Fragrance Journal, (5), 56 - 62, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Yosuke Nishitani, Koji Yamamoto, Masaru Yoshida, Takeshi Azuma, Kazuki Kanazawa, Takashi Hashimoto, Masashi Mizuno

    The flavonoid luteolin is reported to exert anti-inflammatory properties. In this study, we investigated whether luteolin inhibits gut inflammation, using in vivo and in vitro inflammation models. In a dextran sulfate sodium (DSS)-induced colitis mouse model, luteolin (20 and 50 mg/kg) significantly ameliorated shortening of colon length and histological score. Immunohistochemical analysis showed that luteolin also significantly inhibited infiltration of macrophages and interferon (IFN)--producing CD4(+) T cells into the colonic mucosa. Treatment with luteolin also improved IFN- mRNA expression in the colon. At the cellular level, a co-culture consisting of intestinal epithelial Caco-2 and macrophage RAW264.7 cells, stimulated with lipopolysaccharide, the addition of luteolin (100 M) suppressed interleukin (IL)-8 mRNA expression in Caco-2 cells without epithelial monolayer disruption. Expression of tumor necrosis factor (TNF)- protein and proinflammatory cytokines mRNA (TNF-, IL-6, and IL-1) in RAW264.7 cells were also suppressed. HPLC analysis and subsequent cellular assay revealed that aglycone of luteolin was present in the basolateral supernatant of this system at a sufficient concentration to suppress TNF- production and nuclear factor (NF)-B activation of RAW264.7 cells. These results suggest that the luteolin aglycones released from the Caco-2 epithelium inhibits NF-B nuclear translocation in RAW264.7 cells, followed by reduction of TNF- mRNA expression, which results in downregulation of IL-8 mRNA expression in Caco-2 cells. The mechanism by which aglycone inhibits inflammation is important for understanding the roles of luteolin in diet. (c) 2013 BioFactors 39(5):522-533, 2013

    WILEY-BLACKWELL, 2013年09月, Biofactors, 39 (5), 522 - 533, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rendong Ren, Yosuke Azuma, Takao Ojima, Takashi Hashimoto, Masashi Mizuno, Yosuke Nishitani, Masaru Yoshida, Takeshi Azuma, Kazuki Kanazawa

    Laminaria japonica is traditionally eaten in Japan as a beneficial food for thrombosis. The alga contains two specific ingredients, a xanthophyll fucoxanthin (FX) and a polysaccharide, F-fucoidan (FD). The aim of the present study was to investigate whether FX or FD exhibited anti-thrombotic effects. For this purpose, three types of capsules, containing 1 mg FX, 400 mg fucoidan, and both, were prepared from the alga and administered to volunteers for 5 weeks. The dose of FD or FD + FX significantly shortened lysis time (LT) of the thrombus measured by a global thrombosis test in the blood, but FX did not. Examining the mechanism, dietary FD increased H2O2 and the secretion of prostacyclin (PGI(2)), a potent inhibitor of platelet aggregation, in the blood, although FD was under the detection limit in the blood, determining with its monoclonal antibody. Furthermore, in mouse experiments, dietary FD was totally excreted into the faeces and was not incorporated into the blood. We then employed a co-culture system of a Caco-2 cell monolayer with fresh human blood. The addition of FD to Caco-2 cells stimulated the expression of NADPH oxidase 1 (NOX1) and dual oxidase 2 (DUOX2) mRNA and secreted H2O2 onto the blood side accompanied by a significant increase in serum PGI2 production. These effects were invalidated by the combined addition of FD with its monoclonal antibody. The results suggested that dietary FD stimulated the expression of H2O2-producing enzymes in intestinal epithelial cells and released H2O2 into the blood, which played a signalling role to increase PGI(2) production and then shortened LT for thrombi.

    CAMBRIDGE UNIV PRESS, 2013年09月, BRITISH JOURNAL OF NUTRITION, 110 (5), 880 - 890, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Rendong Ren, Takashi Hashimoto, Masashi Mizuno, Hirosato Takigawa, Masaru Yoshida, Takeshi Azuma, Kazuki Kanazawa

    Lipid peroxidation products are known to cause toxicity by reacting with biologically significant proteins, but the inducing role of peroxidation products has been not noted to produce degenerative disease-related eicosanoids. Here, 9-oxononanoic acid (9-ONA), one of the major products of peroxidized fatty acids, was found to stimulate the activity of phospholipase As (PLA2), the key enzyme to initiate arachidonate cascade and eicosanoid production. An exposure of fresh human blood to the atmosphere at 37 degrees C accumulated 9-ONA, increasing peroxide value and thiobarbituric acid reactive substances in the blood. The lipid peroxidation was accompanied by significant increases of PLA(2) activity and thromboxane B-2 (TxB(2)) production, which is a stable metabolite of thromboxane A(2) (TxA(2)) and a potent agonist of platelet aggregation. These events were abolished by standing the blood under nitrogen. The addition of organically synthesized 9-ONA resumed the activity of PLA(2) and the production of Tx132. Also, 9-ONA induced platelet aggregation dose-dependently. These results indicated that 9-ONA is the primary inducer of PLA(2) activity and TxA(2) production, and is probably followed by the development of diseases such as thrombus formation. This is the first report to find that a lipid peroxidation product, 9-ONA, stimulates the activity of PLA(2).

    JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, 2013年05月, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 52 (3), 228 - 233, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yosuke Nishitani, Ling Zhang, Masaru Yoshida, Takeshi Azuma, Kazuki Kanazawa, Takashi Hashimoto, Masashi Mizuno

    Inflammatory bowel disease (IBD) is characterized by chronic inflammation of the gastrointestinal tract. It is unknown whether beta-1,3;1,6-glucan can induce immune suppressive effects. Here, we study intestinal anti-inflammatory activity of Lentinula edodes-derived beta-1,3; 1,6-glucan, which is known as lentinan. Dextran sulfate sodium (DSS)-induced colitis mice were used to elucidate effects of lentinan in vivo. In the cellular level assessment, lentinan was added into a co-culture model consisting of intestinal epithelial Caco-2 cells and LPS-stimulated macrophage RAW264.7 cells. Ligated intestinal loop assay was performed for assessing effects of lentinan on intestinal epithelial cells (IECs) in vivo. Oral administration of lentinan (100 mu g/mouse) significantly ameliorated DSS-induced colitis in body weight loss, shortening of colon lengths, histological score, and inflammatory cytokine mRNA expression in inflamed tissues. Lentinan reduced interleukin (IL)-8 mRNA expression and nuclear factor (NF)-kappa B activation in Caco-2 cells without decreasing of tumor necrosis factor (TNF)-alpha production from RAW264.7 cells. Flow cytometric analysis revealed that surface levels of TNF receptor (TNFR) 1 were decreased by lentinan treatment. A clathrin-mediated endocytosis inhibitor, monodansylcadaverine, canceled lentinan inhibition of IL-8 mRNA expression. Moreover, lentinan inhibited TNFR1 expression in Caco-2 cells in both protein and mRNA level. Lentinan also inhibited TNFR1 mRNA expression in mouse IECs. These results suggest that lentinan exhibits intestinal anti-inflammatory activity through inhibition of IL-8 mRNA expression associated with the inhibition of NF-kappa B activation which is triggered by TNFR1 endocytosis and lowering of their expression in IECs. Lentinan may be effective for the treatment of gut inflammation including IBD.

    PUBLIC LIBRARY SCIENCE, 2013年04月, PLOS ONE, 8 (4), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Immunomodulating compounds in Basidiomycetes

    水野 雅史

    2013年04月, Journal of Clinical Biochemistry and Nutrition, 52 (3), 202 - 207, 英語

    [査読有り][招待有り]

    研究論文(学術雑誌)

  • 水野 雅史

    Agaricus brasiliensis has been demonstrated to have potent antitumor activity. The activity is postulated to act through mediation of the host immune system. We have reported that A. brasiliensis extract (ABE) inhibited compound 48/80 induced a systemic anaphylaxis-like reaction, ear swelling response, and passive cutaneous anaphylaxis-like reaction in mice. There is some recent information available on the mechanism of antiallergic effects resulting from oral administration of ABE. However, information regarding how ABE may activate macrophages through intestinal epithelial cells is still limited. To clarify the mechanism of macrophages activation by ABE, a gut in vitro model constructed of Caco-2 and RAW264.7 cells was applied. Treatment of ABE to the apical compartment resulted in significant increases in tumor necrosis factor (TNF)-α production in the basolateral compartment. Moreover, addition of catalase to the basolateral compartment before ABE treatment suppressed TNF-α production completely, but the addition of superoxide dismutase did not suppress this at all. These data suggest that ABE could potentiate hydrogen peroxide emissions from Caco-2 cells into the basolateral side and activate macrophages, which is important in the immune system. © 2013 Begell House, Inc.

    2013年, International Journal of Medicinal Mushrooms, 15 (4), 365 - 371, 英語

    [査読有り]

    研究論文(学術雑誌)

  • XU Xiaojuan, YASUDA Michiko, MIZUNO Masashi, ASHIDA Hitoshi

    Background: beta-Glucans obtained from fungi, such as baker's yeast (Saccharomyces cerevisiae)-derived beta-glucan (BBG), potently activate macrophages through nuclear factor kappa B (NF kappa B) translocation and activation of its signaling pathways. The mechanisms by which beta-glucans activate these signaling pathways differ from that of lipopolysaccharide (LPS). However, the effects of beta-glucans on LPS-induced inflammatory responses are poorly understood. Here, we examined the effects of BBG on LPS-induced inflammatory responses in RAW264.7 mouse macrophages. Methods: We explored the actions of BBG in RAW264.7 macrophages. Results: BBG inhibited LPS-stimulated nitric oxide (NO) production in RAW264.7 macrophages by 35-70% at concentrations of 120-200 mu g/ml. BBG also suppressed mRNA and protein expression of LPS-induced inducible NO synthase (iNOS) and mitogen-activated protein kinase phosphorylation, but not NF kappa B activation. By contrast, a neutralizing antibody against dectin-1, a beta-glucan receptor, did not affect BBG-mediated inhibition of NO production. Meanwhile, BBG suppressed Pam3CSK-induced NO production. Moreover, BBG suppressed LPS-induced production of pro-and anti-inflammatory cytokines, including interleukin (IL)-1 alpha, IL-1ra, and IL-27. Conclusions: Our results indicate that BBG is a powerful inhibitor of LPS-induced NO production by down-regulating iNOS expression. The mechanism involves inactivation of mitogen-activated protein kinase and TLR2 pathway, but is independent of dectin-1. General significance: BBG might be useful as a novel agent for the chemoprevention of inflammatory diseases. (c) 2012 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2012年10月, Biochmica Biophysica et Acta, General Subject, 1820 (10), 1656 - 1663, 英語

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    研究論文(学術雑誌)

  • Yasuyuki Masuda, Toshinari Takahashi, Kazutoshi Yoshida, Yosuke Nishitani, Masashi Mizuno, Haruhiko Mizoguchi

    Lactic acid bacteria (LAB) in fermented foods have attracted considerable attention recently as treatment options for allergic diseases, the incidence of which has been increasing worldwide. Five strains of LAB isolated from kimoto, the traditional seed mash used for brewing sake, were screened for the ability to suppress IgE-mediated hypersensitivity reaction. Leuconostoc mesenteroides and Lactobacillus sakei, the normal microflora in kimoto, significantly suppressed the reaction, but the contaminant Lactobacillus curvatus did not. Next, we examined the effect of L. sakei LK-117 on atopic dermatitis in the NC/Nga mouse model. LK-117 supplementation significantly reduced the development of atopic dermatitis-like skin lesions in a manner independent of the IgE plasma levels. In the in vitro intestinal model constructed using the human intestinal epithelial cell line Caco-2 and murine macrophage cell line RAW 264.7, treatment with L. sakei LK-117, but not L. curvatus, significantly upregulated TNF-α production from RAW264.7 cells. This result indicated that L. sakei on the apical side affected the macrophages on the basolateral side, and this organism may have the ability to improve allergy symptoms mediated by the intestinal immune system. © 2012 The Society for Biotechnology, Japan.

    2012年09月, Journal of Bioscience and Bioengineering, 114 (3), 292 - 296, 英語

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    研究論文(学術雑誌)

  • Takashi Hashimoto, Yoshiaki Ozaki, Masashi Mizuno, Masaru Yoshida, Yosuke Nishitani, Takeshi Azuma, Akitoshi Komoto, Takashi Maoka, Yuka Tanino, Kazuki Kanazawa

    Dietary fucoxanthin has been reported to exert several physiological functions, and fucoxanthinol is considered to be the primary active metabolite of fucoxanthin. However, there is no information about the pharmacokinetics of fucoxanthinol in human subjects. In the present study, eighteen human volunteers were orally administered kombu extract containing 31 mg fucoxanthin, and their peripheral blood was collected 5 min before and 0·5, 1, 2, 4, 8 and 24 h after the treatment. Plasma fucoxanthinol concentrations were measured by HPLC, and the pharmacokinetics of fucoxanthinol were as follows: maximum concentration, 44·2 nmol/l; time at maximum concentration, 4 h; terminal half-time, 7·0 h; area under the curve (AUC) for 1-24 h, 578·7 nmol/l × h; AUC(∞), 663·7 nmol/l × h. In addition to fucoxanthinol, we also attempted to detect amarouciaxanthin A, a hepatic metabolite of fucoxanthinol, using HPLC, but it was not present in the volunteers' plasma. On the other hand, a peak that was suspected to represent the cis-isomer of fucoxanthinol was found in the HPLC chromatogram. By comparing the present results with those of a previous study using mice, we found that the bioavailability and metabolism of fucoxanthinol differ between human subjects and mice.

    2012年06月, The British journal of nutrition, 107 (11), 1566 - 9, 英語, 国際誌

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    研究論文(学術雑誌)

  • Suk Hyun Choi, Jun-Bae Ahn, Nobuyuki Kozukue, Hyun-Jeong Kim, Yosuke Nishitani, Ling Zhang, Masashi Mizuno, Carol E. Levin, Mendel Friedman

    Partial acid hydrolysis of the tetrasaccharide (lycotetraose) side chain of the tomato glycoalkaloid alpha-tomatine resulted in the formation of four products with three, two, one, and zero carbohydrate side chains, which were separated by high-performance liquid chromatography (HPLC) and identified by thin-layer chromatography (TLC) and liquid chromatography ion-trap time-of-flight mass spectrometry (LCMS-IT-TOF). The inhibitory activities in terms of IC50 values (concentration that inhibits 50% of the cells under the test conditions) of the parent compound and the hydrolysates, isolated by preparative HPLC, against normal human liver and lung cells and human breast, gastric, and prostate cancer cells indicate that (a) the removal of sugars significantly reduced the concentration-dependent cell-inhibiting effects of the test compounds, (b) PC3 prostate cancer cells were about 10 times more susceptible to inhibition by alpha-tomatine than the breast and gastric cancer cells or the normal cells, (c) the activity of alpha-tomatine against the prostate cancer cells was 200 times greater than that of the aglycone tomatidine, and (d) the activity increased as the number of sugars on the aglycone increased, but this was only statistically significant at p < 0.05 for the normal lung He1299 cell line. The effect of the alkaloids on tumor necrosis factor alpha (TNF-alpha) was measured in RAW264.7 macrophage cells. There was a statistically significant negative correlation between the dosage of gamma- and alpha-tomatine and the level of TNF-alpha. alpha-Tomatine was the most effective compound at reducing TNF-alpha. The dietary significance of the results and future research needs are discussed.

    AMER CHEMICAL SOC, 2012年04月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60 (15), 3891 - 3899, 英語

    [査読有り]

    研究論文(学術雑誌)

  • XU Xiaojuan, YASUDA Michiko, NAKAMURA-TSURUTA Sachiko, MIZUNO Masashi, ASHIDA Hitoshi

    Lentinan (LNT), a beta-glucan from the fruiting bodies of Lentinus edodes, is well known to have immunomodulatory activity. NO and TNF-alpha are associated with many inflammatory diseases. In this study, we investigated the effects of LNT extracted by sonication (LNT-S) on the NO and TNF-alpha production in LPS-stimulated murine RAW 264.7 macrophages. The results suggested that treatment with LNT-S not only resulted in the striking inhibition of TNF-alpha and NO production in LPS-activated macrophage RAW 264.7 cells, but also the protein expression of inducible NOS (iNOS) and the gene expression of iNOS mRNA and TNF-alpha mRNA. It is surprising that LNT-S enhanced LPS-induced NF-kappa B p65 nuclear translocation and NF-kappa B luciferase activity, but severely inhibited the phosphorylation of JNK1/2 and ERK1/2. The neutralizing antibodies of anti-Dectin-1 and anti-TLR2 hardly affected the inhibition of NO production. All of these results suggested that the suppression of LPS-induced NO and TNF-alpha production was at least partially attributable to the inhibition of JNK1/2 and ERK1/2 activation. This work discovered a promising molecule to control the diseases associated with overproduction of NO and TNF-alpha.

    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2012年01月, Journal of Biological Chemistry, 287 (2), 871 - 878, 英語

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    研究論文(学術雑誌)

  • Yasuyuki Masuda, Toshinari Takahashi, Kazutoshi Yoshida, Yosuke Nishitani, Masashi Mizuno, Haruhiko Mizoguchi

    Many studies have investigated the immunostimulatory effects of bacteria, such as the anti-allergic effects of lactic acid bacteria (LAB) and LAB-fermented milk. Importantly, these anti-allergic effects have been observed for both viable and nonviable bacteria. However, there are no reported immunological effects of LAB isolated from kimoto, the traditional yeast starter culture used for brewing sake, which also involves spontaneous lactate fermentation. In this study, we determined whether the Leuconostoc mesenteroides and Lactobacillus sakei bacterial strains obtained from kimoto affected the production of interleukin-12 (IL-12), an inducer of the T-helper type-1 immune response. By incubating autoclaved bacteria with J774.1 macrophage-like cells, we found that L. sakei LK-117 induced a sustained increase in IL-12p40 production. The IL-12-inducing ability of LK-117 was unaffected by anti-TLR2 neutralization and was entirely inhibited when the LK-117 cells were treated with RNase. When LK-117 cells were treated with M-1, an N-acetylmuramidase, at varying concentrations and for different periods of time, the ability of the bacteria to induce IL-12 decreased quickly. Although an active fraction could be prepared by chromatography from the soluble products of enzymolysis, the fraction's induction ability was < 2% of that of intact organisms, and induction ability disappeared completely upon anti-TLR2 neutralization after treating the active fraction with RNase. These results suggest that single-stranded RNA released from cells that were disrupted by autoclaving might act as a TLR ligand and provide a novel mechanism in which heat-killed LAB could be used to regulate immune activity. © 2011 The Society for Biotechnology, Japan.

    2011年10月, Journal of Bioscience and Bioengineering, 112 (4), 363 - 368, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Go Bouike, Yosuke Nishitani, Hideyuki Shiomi, Masaru Yoshida, Takeshi Azuma, Takashi Hashimoto, Kazuki Kanazawa, Masashi Mizuno

    To clarify the mechanism of the antiallergic activity of Agaricus blazei Murill extract (ABME), the present paper used an in vivo allergy model and an in vitro intestinal gut model. During OVA sensitization, the serum IgE levels decreased significantly in ABME group. Interleukin (IL)-4 and -5 produced from OVA-restimulated splenocytes was significantly decreased, and anti-CD3 epsilon/CD28 antibody treatment also reduced IL-10, -4, and -5 production and increased IFN-gamma production in ABME group. These results suggest that oral administration of ABME improves Th1/Th2 balance. Moreover, a coculture system constructed of Caco-2 cells and splenocytes from OT-II mice or RAW 264.7 cells indicated that the significant increases in IFN-gamma production by ABME treatment. Therefore, it was concluded that the antiallergic activity of ABME was due to the activation of macrophages by epithelial cells and the promotion of the differentiation of naive T cells into Th1 cells in the immune.

    HINDAWI PUBLISHING CORPORATION, 2011年, EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, Volume 2011, Article ID 532180, 1 - 11, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Izumi Kure, Shin Nishiumi, Yosuke Nishitani, Takeshi Tanoue, Tsukasa Ishida, Masashi Mizuno, Tsuyoshi Fujita, Hiromu Kutsumi, Makoto Arita, Takeshi Azuma, Masaru Yoshida

    Lipoxins, which are bioactive lipids derived from omega-6 polyunsaturated fatty acids, play important roles in various biological functions. In this study, the anti-inflammatory effects of lipoxin A(4) (LXA4; 5S,6R,15S-trihydroxy-7,9,13-trans-11-eicosatetraenoic acid) were investigated in in vitro cultured cell experiments and in vivo animal experiments. In mouse peritoneal macrophages and mouse macrophage cell line RAW264.7 cells, LXA4 reduced the lipopolysaccharide (LPS)-induced increase in the mRNA expression level of tumor necrosis factor (TNF)-alpha. LXA4 also reduced the LPS-induced nuclear translocation of nuclear factor-kappaB (NF-kappaB). In an LPS-induced acute inflammation mouse model, the injection of LXA4 at 5 microg/kg b.wt. led to down-regulation of the TNF-alpha level in serum and the TNF-alpha mRNA expression level in intestinal epithelial cells. Moreover, LXA4 reduced the LPS-caused phosphorylation of IkappaB kinases, IkappaB, and NF-kappaB, the degradation of IkappaB, and the nuclear translocation of NF-kappaB in intestinal epithelial cells. In a coculture system using RAW264.7 cells and human colon carcinoma cell line Caco-2 cells, treatment with LXA4 to Caco-2 cells led to reduction of LPS-evoked TNF-alpha production in RAW264.7 cells and interleukin-8 mRNA expression in Caco-2 cells. These results indicate that LXA4 exerts anti-inflammatory effects through inhibition of NF-kappaB activation, and, therefore, LXA4 may be useful as a therapeutic strategy against intestinal mucosa inflammation.

    2010年02月, The Journal of pharmacology and experimental therapeutics, 332 (2), 541 - 8, 英語, 国際誌

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    研究論文(学術雑誌)

  • Yosuke Nishitani, Takeshi Tanoue, Katsushige Yamada, Tsukasa Ishida, Masaru Yoshida, Takeshi Azuma, Masashi Mizuno

    Probiotics have been used to treat human gastrointestinal inflammations including inflammatory bowel disease (IBD). However, the exact mechanisms by which probiotics act to protect against intestinal inflammation have yet to be fully elucidated. The aim of this study was to evaluate anti-inflammatory effects of Lactococcus lactis subsp. cremoris FC using in vivo and in vitro inflammation models. Colitis was induced in C57BL/6 mice by administration of 3% dextran sulfate sodium to drinking water. In the cellular level assessment, a gut inflammation model with the co-culture system consisting Caco-2 cells and RAW264.7 cells stimulated by LPS was used. Administration of L. lactis subsp. cremoris FC significantly ameliorated shortening of colon length and histological score of the colon in DSS-induce colitis mice. In addition, the treatment of L. lactis subsp. cremoris FC improved the aberrant mRNA expression in inflamed tissue near to control level through notable suppression of TNF-alpha (P<0.05), IFN-gamma (P<0.05), IL-6, iNOS, and MIP-2 mRNA expression. In addition, in a gut inflammation model, treatment with L. lactis subsp. cremoris FC resulted in significant down-regulation of IL-8 mRNA expression in Caco-2 cells and inhibition of NF-kappaB nuclear translocation in RAW264.7 cells. Our findings indicate that administration of L. lactis subsp. cremoris FC improves negative effects of DSS-induced colitis in mice through the inhibition of inflammatory cell infiltration.

    2009年11月, International immunopharmacology, 9 (12), 1444 - 51, 英語, 国際誌

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    研究論文(学術雑誌)

  • Masashi Mizuno, Yosuke Nishitani, Takashi Hashimoto, Kazuki Kanazawa

    A system for assessing the anti-inflammatory effects of food factors was developed by establishing a co-culture system with intestinal epithelial Caco-2 cells and macrophage RAW264.7 cells. The results indicate that fucoidan and lentinan exhibited different suppressive effects on interieukin-8 (IL-8) mRNA expression in Caco-2 through tumor necrosis factor-a (TNF-alpha) production from RAW264.7 stimulated with lipopolysaccharide (LPS).

    TAYLOR & FRANCIS LTD, 2009年10月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73 (10), 2324 - 2325, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masashi Mizuno, Yosuke Nishitani, Takeshi Tanoue, Yoshie Matoba, Taka Ojima, Takashi Hashimoto, Kazuki Kanazawa

    The establishment of a simple technique to determine the concentration of fucoidan wag developed by using a monoclonal antibody against fucoidan. This antibody reacted with fucoidans purified from Laminaria japonica Areschoug (Makombu in Japanese) and Kjellmaniella gyrate Miyabe (Gagome), but not with polysaccharides from Undaria pinnatifida Suringar (Wakame). Neither laminarin nor algenic acid, which are constituents in Laminaria japonica, were recognized by the prepared antibody. Application of the enzymed-linked immunosorbent assay (ELISA) inhibition assay increased the specificity of fucoidan in measuring the fucoidan contents. On the basis of these results, it was ascertained that the ELISA inhibition assay of using the anti-fucoidan monoclonal antibody was rapid, accurate, and sensitive in measuring the content of fucoidan. In addition, the localization of fucoidan in Laminaria japonica was investigated. This is the first report of fucoidan being restricted to the outer cortical layer.

    TAYLOR & FRANCIS LTD, 2009年02月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73 (2), 335 - 338, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takeshi Tanoue, Yosuke Nishitani, Kazuki Kanazawa, Takashi Hashimoto, Masashi Mizuno

    A system for assessing the anti-inflammatory effect of food factors was developed by establishing a co-culture system with intestinal epithelial Caco-2 cells (apical side) and macrophage RAW264.7 cells (basolateral side). In this system, the stimulation of RAW264.7 cells with lipopolysaccharide was followed by a decrease in transepithelial electrical resistance, which is a market of the integrity of the Caco-2 monolayer and an increase in TNF-alpha production from RAW264.7 cells and IL-8 mRNA expression in Caco-2 cells. Treatment with anti-TNF-alpha. antibodies or budesonide suppressed in increase in TNF-alpha production and IL-8 mRNA expression. These results indicated that this co-culture model could imitate the gut inflammation in vivo. In addition, fucoidan, sulphated polysaccharides from brown algae, was employed as a candidate of evolution and added to the apical side of this model. Fucoidan suppressed IL-8 gene expression through a reduction in TNF-alpha production from RAW264.7 cells stimulated with lipopolysaccharide. (C) 2008 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008年09月, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 374 (3), 565 - 569, 英語

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    研究論文(学術雑誌)

  • Hironobu Tsuchida, Masashi Mizuno, Tadahiko Kimura, Akihiro Ohara, Fumie Saito, Tomomi Ito

    'Shibukiri mizu' (SM) is the supernatant containing astringency components obtained from azuki beans that have been boiled in water for 6 min and then allowed to stand. Even though this supernatant is expected to contain many bioactive components, such as polyphenol glycosides, oligosaccharides and saponins, SM is currently disposed as a food-processing waste. In this study, we examined the antibacterial effects of SM on food-poisoning bacteria, plant pathogens, and plant growth-promoting bacteria. SM showed high antibacterial activity to Staphylococcus aureus 209P and Pseudomonas solanacearum MAFF 03- 01418, and very minor activity to Pseudomonas fluorescens MAFF 01-06351. These results indicate that the food processing waste SM may be effectively re-utilized as an antibacterial material against food-poisoning bacteria and plant pathogens.

    2008年, Nippon Shokuhin Kagaku Kogaku Kaishi, 55 (12), 606 - 611, 日本語

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    研究論文(学術雑誌)

  • Tomomi Ito, Hironobu Tsuchida, Akihiro Ohara, Masashi Mizuno, Tadahiko Kimura

    The Japanese phrase, shajuku-eki (SE), refers to the filtrate obtained after re-boiling azuki bean for 90 min to remove the astringency-component during the manufacturing of azuki paste. Currently, SE is discarded as a by-product of azuki paste following treatment with microbes. Here, we investigated the stachyose content in SE and methods to recovery stachyose for the purpose of effectively reusing SE. We found that the stachyose content in SE was 25.9-49.3% that of the total stachyose content in azuki beans of 5 cultivars, and that the carbonation process showed the highest recovery rate of stachyose (70.6-80.0% yield) with 75.5-80.5% decolorization. These results indicate that effective re-utilization of SE may be possible.

    2008年, Nippon Shokuhin Kagaku Kogaku Kaishi, 55 (10), 506 - 509, 日本語

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    研究論文(学術雑誌)

  • Takanobu Morimoto, Michihiro Takagi, Masashi Mizuno

    We examined the remedial effect of Agaricus brasiliensis S. Wasser et al. (ABSW) on the severity of dermatitis and levels of serum immunoglobulin E (IgE) in NC/Nga mice, which develop atopic dermatitis-like skin lesions on treatment with repeated challenge with picryl chloride (PiCl). Oral administration of ABSW extract suppressed the development of dermatitis. We also investigated the levels of serum IgE and found that the levels in the ABSW group were lower than in the control group. Moreover, serum levels in the ABSW group were increased. We further investigated ex vivo interferon (IFN)-γ and interleukin (IL)-4 production from spleen cells on stimulation with concanavalin A or a simultaneous treatment of anti-CD3ε and anti-CD28 antibodies. There were no significant differences between ABSW and control groups in the production of IFN-γ and IL-4. ABSW extract directly increased IFN-γ production and decreased IL-4 production in cytokine production from spleen cells in vitro. These results suggest that ABSW establishes Th1 dominance, which contributes to cellular immunity. © 2008 by Begell House, Inc.

    2008年, International Journal of Medicinal Mushrooms, 10 (1), 15 - 24, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Degradation of cell wall polysaccharides during postharvest fruit ripening and softening of different apple varieties

    Chang-Hai Jin, Masashi Mizuno, Juan Kan, Biao Suo, Zhi-Jun Wang, Hironobu Tsuchida

    The cell wall material (CWM) and eight cell wall polysaccharides fractions were extracted from 'Fuji' and 'Kinsei' apples during storage at different time (0 and 42 days). The sugar composition characteristics of each fraction were determined by gas chromatography. The results showed that, during storages, the firmness of 'Kinsei' apples decreased significantly (Fig. 1), and a significant peak of ethylene production was shown after 10 d storage, but only a little ethylene was produced in 'Fuji' apples, which had a better storability (Fig.2). Compare to other cell wall polysaccharide fractions, in Na2CO 3-soluble pectic fractions of apple fruit, there were abundant rhamnogalacturonan I (RG-I), which branched highly in side chains due to the compositions of arabinans, galactans, arabinogalactans etc (Tables 2-5 and Fig.4). As for cell wall polysaccharides, in 'Kinsei' apples, the decrease of pectic fractions was shown most significantly in Na2CO3-1 fraction (Table 1 and Fig.3), which was associated with a significant degradation of arabinosyl and galactosyl residues on the side chains (Table 3). Further more, higher molecular mass in Na2CO3-1 pectic polysaccharides degraded and turned into ones with smaller molecular mass (Fig.5). From these results, the degradation of side chains in Na 2CO3-1 pectic polysaccharides under the activity of enzyme was considered one of the most significant factors of apple fruit softening through modifying the network of cell wall polysaccharides.

    2006年12月, Journal of Plant Physiology and Molecular Biology, 32 (6), 617 - 626, 中国語

    [査読有り]

    研究論文(学術雑誌)

  • Masashi Mizuno, Sachiko Kawakami

    Peritoneal macrophages from toll-like receptor (TLR) 4 and TLR2 knockout mice (TLR4-/- and TLR2-/-) were stimulated with a water-insoluble hetero-glycan (ABHG), purified from a fruit body of Agaricus brasiliensis. TLR4-/- mice did not produce tumor necrosis factor (TNF)-α and nitric oxide (NO), but TLR2-/- did. However, ABHG did not inhibit TNF-α and NO production by macrophages in the presence of polymyxin B, which could bind to lipid A in lipopolysaccharide (LPS) suggesting that ABHG did not contain LPS as contaminants. These results indicated that ABHG was recognized through TLR4-/- to produce inflammatory cytokines such as TNF-α and NO. RT-PCR was used to investigate the effect of ABHG on mRNA expression of TNF-α and inducible nitric oxide synthase (iNOS) in RAW 264.7. TNF-α mRNA expression was induced 0.5 hr after stimulation, peaked in 3 hr, and thereafter decreased to reach a stable level after 24 hr. On the other hand, iNOS mRNA induction was delayed for 3 hr and thereafter remained at the same level until 24 hr after stimulation. Furthermore, the DNA binding activity of NF-κB was observed as early as 0.5 hr after treatment and rapidly increased to its peak value 2 hr later, and thereafter decreased. When RAW 264.7 was stimulated with ABHG in the presence of an anti-TNF-α antibody, TNF-α mRNA expression was not affected and iNOS was downregulated. These results indicate that TNF-α production by RAW 264.7, stimulated with an immunomodulating polysaccharide such as ABHG, was enhanced by upregulating of NF-κB through TLR4, and the newly produced TNF-α enhanced NO production trough an autocrine pathway. © 2006 by Begell House, Inc.

    2006年, International Journal of Medicinal Mushrooms, 8 (3), 223 - 229, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y Sakamoto, K Minato, M Nagai, M Mizuno, T Sato

    Lentinan, an antitumor substance purified from Lentinula edodes, is degraded during post-harvest preservation as a result of increased glucanase activity. We isolated an exo-beta-1,3-glucanase encoding gene, exg2, from L. edodes which is a homologue of an exo-glucanase-encoding gene conserved in ascomycetous fungi. The exg2 gene was cloned as an approximately 2.4-kbp cDNA, and as a genomic sequence of 3.9-kbp. The product of the exg2 gene is predicted to contain 759 amino acids with a molecular weight of 79 kDa and a pI value of 4.6. The putative N-terminus of EXG2 is identical to the N-terminal sequences of lentinan-degrading enzymes, GNase I and II, and a custom-made anti-EXG2 peptide anti-serum cross-reacted with purified GNase I and II. Transcription and translation of exg2 was low in the gills of mature fruiting bodies, but increased after harvesting. We conclude that the exg2 gene is a lentinan-degrading enzyme-encoding- gene in L. edodes.

    SPRINGER, 2005年09月, CURRENT GENETICS, 48 (3), 195 - 203, 英語

    [査読有り]

    研究論文(学術雑誌)

  • M Mizuno, Y Tada, K Uchii, S Kawakami, S Mayama

    In potato (Solanum tuberosum L.) suspension cells, the expression of the gene encoding alternative oxidase (AOX) and H2O2 accumulation were induced by treatment with β-glucan elicitor. The inhibition of catalase activity enhanced both AOX mRNA expression and the production of H2O2, whereas the ascorbate peroxidase inhibitor did not have any effect on these responses. Simultaneous inhibition of catalase and AOX activities in elicited cells dramatically increased H2O2 accumulation, leading to the disruption of mitochondrial membrane potential (&UDelta;ψ(m)) and programmed cell death (PCD). The results demonstrate, for the first time, that not only AOX but also catalase plays a central role in the suppression of mitochondrial &UDelta;ψ(m) breakdown and PCD induced by β-glucan elicitor.

    SPRINGER, 2005年04月, PLANTA, 220 (6), 849 - 853, 英語

    研究論文(学術雑誌)

  • The expression profile of lectin differs from that of seed storage proteins in Castanea crenata trees

    S Nakamura, A Ikegami, M Mizuno, F Yagi, K Nomura

    Using Northern blot analysis, the expression of the Japanese chestnut (Castanea crenata Sieb. et Zucc.) agglutinin (CCA) gene was compared with that of its seed storage protein (SSP) gene. After cDNA cloning of SSP, the expression profile of SSP mRNA and CCA mRNA were compared. SSP mRNA was seed-specific, while CCA mRNA was expressed in the stems and flowers (both male and female) as well as in the seeds. Whereas extracts from all organs observed using Western blot analysis exhibited positive signals, in seeds, large expressions of SSP mRNA were restricted to the late maturation and harvest stages. Levels were maintained during the dormant period. No expression was observed during the germination stage. In contrast, CCA mRNA expression was maintained at a high level during development, was at a relatively low level during dormancy, and showed subsequent high expression during germination. These results suggest that one of the physiological roles of CCA is to act as a vegetative storage protein. But since protein expression did not coincide with that of mRNA, the expression of CCA may be regulated both at the transcription and the translation levels.

    TAYLOR & FRANCIS LTD, 2004年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68 (8), 1698 - 1705, 英語

    研究論文(学術雑誌)

  • The expression profile of lectin differs from that of seed storage proteins in Castanea crenata trees

    S Nakamura, A Ikegami, M Mizuno, F Yagi, K Nomura

    Using Northern blot analysis, the expression of the Japanese chestnut (Castanea crenata Sieb. et Zucc.) agglutinin (CCA) gene was compared with that of its seed storage protein (SSP) gene. After cDNA cloning of SSP, the expression profile of SSP mRNA and CCA mRNA were compared. SSP mRNA was seed-specific, while CCA mRNA was expressed in the stems and flowers (both male and female) as well as in the seeds. Whereas extracts from all organs observed using Western blot analysis exhibited positive signals, in seeds, large expressions of SSP mRNA were restricted to the late maturation and harvest stages. Levels were maintained during the dormant period. No expression was observed during the germination stage. In contrast, CCA mRNA expression was maintained at a high level during development, was at a relatively low level during dormancy, and showed subsequent high expression during germination. These results suggest that one of the physiological roles of CCA is to act as a vegetative storage protein. But since protein expression did not coincide with that of mRNA, the expression of CCA may be regulated both at the transcription and the translation levels.

    TAYLOR & FRANCIS LTD, 2004年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68 (8), 1698 - 1705, 英語

    研究論文(学術雑誌)

  • K Minato, S Kawakami, K Nomura, H Tsuchida, M Mizuno

    Two beta-1,3-glucanase isoenzymes, GNase I and 11, were isolated from Lentinule edodes fruiting body. They degraded lentinan in an exo manner during storage of the mushroom. GNase I and 11 were monomeric proteins with apparent molecular masses of 72.9 and 74.7 kDa, respectively. It was shown that K-m, values for GNase I and GNase 11 against lentinan were 1.02 and 2.41 muM, respectively. Moreover, it was demonstrated by western blotting analysis that this enzyme was synthesized de novo in L. edodes during storage at 20 degreesC, but not at I or 5 degreesC. And this pattern of western blot was consistent with that of glucanase activity during storage of L. edodes. In addition, the immunomodulating effects of lentinan on TNF-alpha and NO production were diminished with its degradation by this enzyme. These results suggested that synthesis of exo beta-1,3-glucanases were induced under storage conditions at 20 degreesC, and lentinan degradation by their attack might result in the depletion of immunomodulating activity of L. edodes. (C) 2004 Elsevier Ltd. All riahts reserved.

    ELSEVIER SCI LTD, 2004年07月, CARBOHYDRATE POLYMERS, 56 (3), 279 - 286, 英語

    研究論文(学術雑誌)

  • Fukuda, I, S Nishiumi, Y Yabushita, R Mukai, R Kodoi, K Hashizume, M Mizuno, Y Hatanaka, H Ashida

    Halogenated aromatic hydrocarbons (HAHs), such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), produce a wide variety of biological and toxic effects mainly through the aryl hydrocarbon receptor (AhR)-dependent mechanism. After the binding of HAHs, the AhR subsequently transforms its form in order to interact with a specific DNA sequence, the dioxin responsive element (DRE). Thus, detection of the transformed AhR is a target for estimation of the biological and toxic potency of ligands. In this study, we have developed a simple method for quantitative assessment of the transformation state of AhR based on an enzyme-linked immunosorbent assay (ELISA) combined with southwestern chemistry technique (SW-ELISA) that detects the complex of transformed AhR:fluorescein isotbiocyanate (FITC)-labeled DRE probe. SW-ELISA has shown the response to HAHs including TCDD and other known agonists in a dose-dependent manner. In the case of TCDD, SW-ELISA has revealed a minimum detection limit (MDL) of 2 pM (0.026 pg/assay), a median effective concentration (EC50) value of 0.125 nM (1.6 pg/assay), and a maximum response at 10 nM (129 pg/assay). Furthermore, SW-ELISA provides the confirmation that flavonoids, the potent antagonists for AhR as reported previously, show the inhibitory effects on TCDD-induced AhR transformation. These results indicate that SW-ELISA is a new and straightforward method for the detection of AhR transformation and will be useful in screening of agonists or antagonists for AhR. (C) 2004 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2004年04月, JOURNAL OF IMMUNOLOGICAL METHODS, 287 (1-2), 187 - 201, 英語

    研究論文(学術雑誌)

  • Lentinan from shiitake mushroom (Lentinus edodes) suppresses expression of cytochrome P450 1A subfamily in the mouse liver

    T Okamoto, R Kodoi, Y Nonaka, Fukuda, I, T Hashimoto, K Kanazawa, M Mizuno, H Ashida

    Induction of xenobiotics metabolizing enzymes is related to the formation and chemoprevention of cancer. Since cytochrome P450s (CYPs) including CYP1A subfamily metabolize certain pro-carcinogens to their ultimate forms, downregulation of CYP1As by food factors leads to the prevention of cancer. Mushroom polysaccharides, especially beta-glucans such as lentinan from Lentinus edodes, possess the anti-tumor and immunomodulating activities through the cytokine production from immunocytes. Recent our studies have demonstrated that lentinan suppresses hepatic CYP1As expression in the both constitutive and inducible levels through the production of tumor necrosis factor-a and an increase in the DNA-binding activity of nuclear factor-kappaB. This paper discusses on the effective lentinan dosage and route of administration for suppression of CYP1As.

    WILEY-BLACKWELL, 2004年, BIOFACTORS, 21 (1-4), 407 - 409, 英語

    研究論文(学術雑誌)

  • Changes of lentinan contents and glucanase activity in Lentinus edodes stored under controlled atmosphere.

    KAWAKAMI S, MINATO K, TOKIMOTO K, FUJITAKE Nobuhide, MIZUNO Masashi

    2004年, Int. J. Medic. Mushrooms., 6, pp.57-62, 英語

    研究論文(学術雑誌)

  • Changes of lentinan contents and glucanase activity in Lentinus edodes stored under controlled atmosphere

    KAWAKAMI S, MINATO K, TOKIMOTO K, FUJITAKE Nobuhide, MIZUNO Masashi

    2004年, Int. J.Medic. Mushrooms, 6, 未記入, 英語

    研究論文(学術雑誌)

  • Catalase and alternative oxidase cooperatively regulate programmed cell death induced by □-glucan elicitor in potate suspension cultures.

    MIZUNO Masashi, TADA Y, UCHII K, KAWAKAMI S, MAYAMA Shigeyuki

    2004年, Planta, online journal., 英語

    研究論文(学術雑誌)

  • An exo β-1, 3-glucanase synthesized de novo degrades lentinan during storage of Lentinule edodes and diminishes immunomodulating activity of the mushroom.

    MINATO K, KAWAKAMI S, NOMURA K, TSUCHIDA H, MIZUNO Masashi

    2004年, Carbohydr. Polym., 56, pp.279-286, 英語

    研究論文(学術雑誌)

  • Suppression of cytochrome P4501 a subfamily in mouse liver by oral intake of polysaccharides from mushrooms, Lentinus edodes and Agaricus blazei

    H Ashida, T Hashimoto, Y Nonaka, Fukuda, I, K Kanazawa, G Danno, K Minato, S Kawakami, M Mizuno

    Mushrooms have been consumed as flavorful and medicinal foods for millenniums. Mushroom polysaccharides possess antitumor activity through the stimulation of cytokine productions from immunocytes. On the other hand, lipopolysaccharide suppresses the expression of various hepatic cytochrome P450s (CYPs) through the production of cytokines such as tumor necrosis factor (TNF)-alpha. In this study, lentinan prepared from Lentinus edodes and polysaccharides from Agaricus blazei (ABPS) were intraperitoneally injected to female BALB/c mice, and the effects of these polysaccharides on the expression of CYPs were investigated in the liver. Both polysaccharides down-regulated the activity and level of constitutive and 3-methylcholanthrene-inducible CYP1A accompanied by the TNF-alpha production. When lentinan was orally administered to mice, down-regulation of the level and activity of CYP1A through the suppression of DNA binding activity of aryl hydrocarbon receptor also occurred. These results suggest that the mushroom polysaccharides such as lentinan and ABPS have an anti-carcinogenic activity, since the down-regulation of CYP1A is considered to prevent the metabolic activation of procarcinogens.

    AMER CHEMICAL SOC, 2003年, FOOD FACTORS IN HEALTH PROMOTION AND DISEASE PREVENTION, 851, 235 - 248, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Preventive effects of food components on caspase-8-mediated apoptosis induced by dietary carcinogen, Trp-P-1, in rat mononuclear cells

    T Hashimoto, W Ito, T Furuyashik, T Sano, K Minato, M Mizuno, K Kanazawa, G Danno, H Ashida

    3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a contaminant in diet, induces apoptosis in immunocytes such as splenocytes and thymocytes. In rat mononuclear cells (MNCs), Trp-P-1 also induces apoptosis, in which caspase-8 dominated the caspase cascade. Since Trp-P-1-induced apoptosis in immunocytes may be led to immunodeficiency, it is important for a living body to prevent the apoptosis. Although Trp-P-1 increased the generation of reactive oxygen species, both ascorbic acid and alpha-tocophrol did not inhibite the apoptosis. This indicates that the induction of apoptosis was not mediated through reactive oxygen species. On the other hand, certain food components such as dopamine, myricetin, robinetin, delphinidin, pyrogallol, and gallic acid inhibited Trp-P-1-induced apoptosis. Thus, these food components in our dairy diet may prevent chemical-induced cytotoxicity in immunocytes.

    AMER CHEMICAL SOC, 2003年, FOOD FACTORS IN HEALTH PROMOTION AND DISEASE PREVENTION, 851, 128 - 140, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Macrophages stimulated with polysaccharide purified from Agaricus brasiliensis enhance mRNA expression of Th1 cytokine including IL-12 and 18

    MIZUNO Masashi, KAWAKAMI S, SAKAMOTO Y, FUJITAKE Nobuhide

    2003年, Int. J. Med. Mushrooms, 5, 397-403, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Macrophages stimulated by polysaccharide purified from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae) enhance mRNA expression of Th1 cytokineincluding IL-12 and 18

    MIZUNO Masashi, KAWAKAMI S, SAKAMOTO Y, FUJITAKE Nobuhide

    2003年, Int. J. Medic.Mushrooms, 5,pp.397-403., 英語

    研究論文(学術雑誌)

  • Molecular cloning of ascorbate peroxidase in potato tubers and its response during storage at low temperature

    S Kawakami, Y Matsumoto, A Matsunaga, S Mayama, M Mizuno

    To investigate the response of ascorbate peroxidase (APx; EC 1.11.1.11) to low-temperature stress in potato tubers, a cDNA clone encoding APx was isolated from a potato tuber eDNA library. The full-length clone contains a cDNA that is 1039 bp in length and has an open reading frame of 750 by coding for 250 amino acids. The deduced amino acid sequence of APx had high homology with the cytosolic APx (cAPx) from other plant sources and lacked a transit peptide, implying that it encoded a cAPx. APx mRNA was detected by northern blot analysis using cAPx as a DNA probe. mRNA levels during storage at 1 degreesC increased 2.5-fold in 3 weeks as compared with the initial values. Activity staining of APx peaked at 12 weeks after harvest in tubers stored at 1 degreesC, and increased approximately 3.4-fold during the first 12 weeks of storage. APx amounts as determined by western blot analysis using anti-APx antibody correlated well with activity staining of APx. This shows that APx was highly induced by low temperature stress during storage. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

    ELSEVIER IRELAND LTD, 2002年10月, PLANT SCIENCE, 163 (4), 829 - 836, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Suppressive effect of polysaccharides from the edible and medicinal mushrooms, Lentinus edodes and Agaricus blazei, on the expression of cytochrome P450s in mice

    T Hashimoto, Y Nonaka, K Minato, S Kawakami, M Mizuno, Fukuda, I, K Kanazawa, H Ashida

    To investigate the effects of lentinan from Lentinas edodes and polysaccharides from Agaricus blazei (ABPS) on the expression of cytochrome P450s (CYPs), lentinan (10 mg/kg/day) or ABPS (200 mg/kg/day) was administered to female BALB/c mice four times every other day by intraperitoneal injection. Lentinan and ABPS suppressed both the constitutive and 3-methylcholanthrene-induced CYP1A expression and ethoxyresorufin-O-deethylation activity in the liver.

    TAYLOR & FRANCIS LTD, 2002年07月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66 (7), 1610 - 1614, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masashi Mizuno, Ken-Ichiro Minato, Sachiko Kawakami, Shigenobu Tatsuoka, Yutaka Denpo, Hironobu Tsuchida

    We measured the contents of anti-tumor polysaccharides in certain mushrooms using the two kinds of antibodies that specifically recognize lentinan and GGF, which were isolated from Lentinus edodes and Grifola frondosa, respectively, as β-glucans of these polysaccharides. To determine which mushrooms showed immunomodulating activity, the TNF-α and NO productions of mouse peritoneal macrophages and the subset analysis of splenic lymphocytes stimulated with a polysaccharide from each mushroom were examined. It was ascertained that polysaccharides from mushrooms that enhanced NO production also elicited TNF-α production and that mushrooms containing the positive polysaccharides to anti-lentinan and anti-GGF antibodies at least augmented the production of TNF-α and NO. Furthermore, the polysaccharides from Fammulina velutipes and Meripilus giganteu increased lymphocyte T cell subsets. Therefore, we demonstrated that those polysaccharides had high levels of immunomodulating activity.

    S. Karger AG, 2001年, Food Science and Technology Research, 7 (1), 31 - 34, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masashi Mizuno

    Japanese Society for Food Science and Technology, 2001年, Nippon Shokuhin Kagaku Kogaku Kaishi, 48 (11), 793 - 797, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • S Kawakami, M Mizuno, H Tsuchida

    We compared the antioxidant enzyme activities between Solanum tuberosum L. cultivars Kitaakari and "Danshaku" during storage at 1 degrees C and 20 degrees C. The Kitaakari and Danshaku plants contained approximately 330 mu M and 120 mu M ascorbic acid (AsA) immediately after the harvest, respectively. At 1 degrees C, the activity of ascorbate peroxidase (APx) in the Kitaakari plants showed the tendency to increase, while in the Danshaku its activity increased temporally by 9 weeks and thereafter returned to basal levels. Superoxide dismutase (SOD) activity increased after 12 weeks in the case of the Kitaakari at 1 degrees C. Catalase did not show any difference in both cultivars at each temperature. The contents of AsA, which was one of the substrates of APx, decreased more rapidly at 1 degrees C than at 20 degrees C in both cultivars. Particularly in the case of the Danshaku, AsA contents were already less than 30 mu M at 9 weeks, which confirmed that APx was inactivated.

    AMER CHEMICAL SOC, 2000年06月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 48 (6), 2117 - 2121, 英語

    [査読有り]

    研究論文(学術雑誌)

  • M. Mizuno

    The changes in the contents of an anti-tumor polysaccharide from Lentinus edodes (lentinan) and Grifola frondosa (GGF) during storage were investigated using by an ELISA inhibition assay. When the mushrooms were stored at low temperature, the contents of their anti-tumor polysaccharides show hardly any changes, but their contents decreased markedly at higher temperature (20°C). Moreover, the effect of the extract from Lentinus edodes stored at different temperatures on tumor necrosis factor (TNF)-α and nitric oxide (NO) productions from macrophages was investigated to confirm the influence to the stimulation of macrophages. Because lentinan stimulates macrophages to augment their antitumor activity. Their productions showed little difference between Lentinus edodes stored at low temperature and the fresh mushroom, although the cytokine production decreased significantly in Lentinus edodes stored at 20°C. These results suggest that low-temperature storage is more effective in maintaining not only the quality of the mushrooms but also the contents of anti-tumor polysaccharides as health-beneficient foods.

    IOS Press, 2000年, BioFactors, 12 (1-4), 275 - 281, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Anti-tumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei mill

    Masashi Mizuno, Ken-Ichiro Minato, Hitoshi Ito, Mitsuo Kawade, Hirofumi Terai, Hironobu Tsuchida

    Anti-tumor active polysaccharide against Sarcoma 180 was isolated by DEAE-Sepharose CL-6B and Sepharose 4B column chromatography from the hot-water soluble fraction of the mycelium of liquid-cultured Agaricus blazei mill. This polysaccharide did not react with antibodies of anti-tumor polysaccharides such as lentinan, gliforan, and FIII-2-b which is one of anti-tumor polysaccharides from Agaricus blazei. Moreover, the analyses of 13C-NMR and GC-MS suggested that this polysaccharide was preliminarily glucomannan with a main chain of β-1,2-linked D-mannopyranosyl residues and B-D-glucopyranosyl-3-O-β-D-glucopyranosyl residues as a side chain. This polysaccharide was completely different from the anti-tumor polysaccharide from fruiting body of Agaricus blazei, β-1,6-glucan.

    1999年04月, Biochemistry and Molecular Biology International, 47 (4), 707 - 714, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masashi Mizuno, Akio Yamakawa, Ken-Ichiro Minato, Sachiko Kawakami, Shigenobu Tatsuoka, Hirofumi Terai, Hironobu Tsuchida

    Antibodies against an antitumor β-glucan purified from Grifola frondosa (GGF) were raised in the rabbit by subcutaneous immunization. Our antibodies reacted significantly with GGF by an ELISA inhibition assay. The antibodies did not recognize other polysaccharides such as laminarin and pustulan, but reacted somewhat with lentinan, whose structure is similar to GGF. It was demonstrated that GGF could be measured by ELISA using antibodies. In addition, the effects of the storage temperature on GGF content during storage were measured using our antibody. GGF content was 24.7 μg/g fresh weight (f.w.) at zero time storage, and little change occurred during storage of the mushroom for 7 days at 5°C. However, a drastic decrease to 11.4 μg/g f.w. occurred after 7 days of storage at 20°C. These results suggest that storage at low temperatures is desirable to maintain the quality of GGF.

    S. Karger AG, 1999年, Food Science and Technology Research, 5 (4), 398 - 401, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Gingko biloba extract EGb 761 is a suppresser of AP-1 transcription factor stimulated by phorbol 12-myristate 13-acetate

    Masashi Mizuno, Marie-Therese Droy-Lefaix, Lester Packer

    Transcription factors are activated in response to a wide variety of extracellular stimuli. The present study investigated which step Gingko biloba extract EGb 761), a flavonoid phytochemical rich preparation can affect signal transduction during AP-1 activation. Kinetic experiments with human Jurkat T-cell lines demonstrate that it takes 1 to 1.5 h to activate AP-1 DNA binding activity. These data indicate that c-fos mRNA is expressed and then AP-1 DNA binding activity is activated. Pretreatment of Jurkat T cells with 10 μg/ml EGb 761 suppresses AP-1 DNA activation and c-fos mRNA expression. These results suggest that the step in the signal transduction pathway for AP-1 activation that is inhibited by EGb 761 is upstream to c-fos mRNA expression.

    1996年, Biochemistry and Molecular Biology International, 39 (2), 395 - 401, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Masashi Mizuno, Junko Yamada, Hirofumi Terai, Nobuyuki Kozukue, Yong Shun Lee, Hironobu Tsuchida

    The different effects between wild and cultured Panax ginseng on immunological activity were investigated. The extracts of hot water soluble fraction from wild Panax ginseng showed the mitogenic activity to lymphocytes but that from cultured Panax ginseng did not. The mitogenic activity of wild Panax ginseng (100 μg/well) was almost equal to Concanavalin A (0.1 μg/well) which was well-known as one of T cell mitogens. The percentages of Thy 1.2-(pan T cells), L3T4-(helper T cells) and Lyt2-(cytotoxic T cells) positive cell population were significantly increased in the mice orally administered hot water soluble fraction from wild Panax ginseng as compared to control by 31.2, 17.9 and 30.1 percent, respectively. © 1994 Academic Press, Inc.

    1994年05月30日, Biochemical and Biophysical Research Communications, 200 (3), 1672 - 1678, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A MUTAGEN FROM HISTIDINE REACTED WITH NITRITE

    G DANNO, K KANAZAWA, M TODA, M MIZUNO, H ASHIDA, M NATAKE

    Mutagenicity of the reaction products of L-histidine with sodium nitrite at pH 4.0 were studied. One of the five reaction products obtained showed the mutagenicity in Salmonella typhimurium his reversion assay. The mutagen was purified by crystallization and identified by instrumental analyses to be (1-nitroso-1H-imidazol-4-yl)acetohydroxamic acid (NIAH), which was the product of histidine undergoing nitrosation at the imidazolic secondary amine and oxidation of the primary amine at the side chain. NIAH showed a lethal mutation on TA98 strain without S9 mix and then was submitted to the induced mutation frequency (IMF) test. NIAH gave a dose-dependent IMF value on TA98, and the value at the concentration of 10 mug/plate was 4.29 X 10(-6)/cell. NIAH exhibited weak mutagenicity on TA100 strain and did not have a DNA-damaging activity on H17 Rec+ and M45 Rec- of Bacillus subtilis. Therefore, NIAH was considered to be a frame-shift-type mutagen.

    AMER CHEMICAL SOC, 1993年07月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 41 (7), 1090 - 1093, 英語

    [査読有り]

    研究論文(学術雑誌)

  • BUTYLATED HYDROXYANISOLE PRODUCES BOTH MUTAGENIC AND DESMUTAGENIC DERIVATIVES UNDER GASTRIC CONDITIONS

    K KANAZAWA, M MIZUNO

    Dietary butylated hydroxyanisole (BHA) has been known to have inconsistent functions on carcinogenesis, both prevention ard initiation. We assumed that both functions of BHA were introduced by the derivatives formed after the reaction with gastric components such as nitrite in the stomach. We then identified the derivatives produced by incubating BHA with sodium nitrite at pH 2.0 or pH 5.0. Eight derivatives were detected; 2-tert.-butyl-p-quinone (BQ), 3,3'-di-tert.-butyl-biphenyldiquinone-(2,5,2',5') (BBDQ), 2,6-di-tert.-butyl-8-hydroxy-dibenzofuran-1,4-quinone (BHDQ), 6-nitro-BHA, 2,2'-dihydroxy-3,3'-di-tert.butyl-5,5'-dimethoxy-biphenyl (di-BHA), an oxidized product of di-BHA, and two unstable reaction intermediates. BQ was a major final product at pH 2.0, but not at pH 5.0. 6-Nitro-BHA and the oxidized products of di-BHA were also the final products. BBDQ was formed from di-BHA and easily converted to BHDQ. Their mutagenicity and desmutagenicity were assayed using Salmonella typhimurium strains. BQ and BBDQ were the mutagens of base-substitution type, BHDQ was the potent desmutagen against a mutagenicity of Trp-P-2, and the others had neither of the two activities. Thus, BHA was found to produce both the mutagen and desmutagen under the gastric conditions. BQ has been previously reported to be easily detoxified by glutathione, and BHA itself is well known to prevent carcinogenesis. In the assessment of dietary BHA on carcinogenesis, since one of the mutagens from BHA is easily detoxified in our bodies and another is converted to a desmutagen, BHA appears to be one of the favourable chemicals for us.

    BIOSCIENCE EDIPRINT INC, 1992年, INTERNATIONAL JOURNAL OF TISSUE REACTIONS-EXPERIMENTAL AND CLINICAL ASPECTS, 14 (5), 211 - 218, 英語

    [査読有り]

    研究論文(学術雑誌)

  • WHAT DO THIOBARBITURIC ACID AND HEMOGLOBIN-METHYLENE BLUE TESTS EVALUATE IN THE ENDOGENOUS LIPID-PEROXIDATION OF RAT-LIVER

    K KANAZAWA, N INOUE, H ASHIDA, M MIZUNO, M NATAKE

    JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, 1989年07月, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 7 (1), 69 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • DEPLETION OF HEPATIC COENZYME-A DERIVATIVES IS ONE OF THE MARKERS OF THE TOXICITY OF ORALLY-ADMINISTERED SECONDARY AUTOXIDATION PRODUCTS OF LINOLEIC-ACID IN RAT

    K KANAZAWA, H ASHIDA, M MIZUNO, M NATAKE

    CENTER ACADEMIC PUBL JAPAN, 1989年02月, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 35 (1), 11 - 23, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • Masashi Mizuno, Takashi Hashimoto

    ELSEVIER SCIENCE INC, 2014年11月, FREE RADICAL BIOLOGY AND MEDICINE, 76, S63 - S63, 英語

    研究発表ペーパー・要旨(国際会議)

  • Masashi Mizuno, Yosuke Nishitani

    ELSEVIER SCIENCE INC, 2012年11月, FREE RADICAL BIOLOGY AND MEDICINE, 53, S77 - S77, 英語

    研究発表ペーパー・要旨(国際会議)

  • Anti-inflammatory activity of Shiitake mushroom-derived b-1,3;1,6-glucan, lentinan, through modulation of TNF receptor 1 (TNFR1) distribution in intestinal epithelial cells

    Yosuke Nishitani, Masashi Mizuno

    AMER CHEMICAL SOC, 2012年08月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 244, 英語

    研究発表ペーパー・要旨(国際会議)

  • Chikako Nagasato, Akira Inoue, Masashi Mizuno, Kazuki Kanazawa, Takao Ojima, Kazuo Okuda, Taizo Motomura

    During cytokinesis in brown algal cells, Golgi-derived vesicles (GVs) and flat cisternae (FCs) are involved in building the new cell partition membrane. In this study, we followed the membrane fusion process in Silvetia babingtonii zygotes using electron microscopy together with rapid freezing and freeze substitution. After mitosis, many FCs were formed around endoplasmic reticulum clusters and these then spread toward the future cytokinetic plane. Actin depolymerization using latrunculin B prevented the appearance of the FCs. Fusion of GVs to FCs resulted in structures that were thicker and more elongated (EFCs; expanded flat cisternae). Some complicated membranous structures (MN; membranous network) were formed by interconnection of EFCs and following the arrival of additional GVs. The MN grew into membranous sacs (MSs) as gaps between the MNs disappeared. The MSs were observed in patches along the cytokinetic plane. Neighboring MSs were united to form the new cell partition membrane. An immunocytochemical analysis indicated that fucoidan was synthesized in Golgi bodies and transported by vesicles to the future cytokinetic plane, where the vesicles fused with the FCs. Alginate was not detected until the MS phase. Incubation of sections with cellulase-gold showed that the cellulose content of the new cross wall was not comparable to that of the parent cell wall.

    SPRINGER, 2010年07月, PLANTA, 232 (2), 287 - 298, 英語

    書評論文,書評,文献紹介等

  • Yosuke Nishitani, Masashi Mizuno

    ELSEVIER SCIENCE INC, 2010年, FREE RADICAL BIOLOGY AND MEDICINE, 49, S147 - S147, 英語

    研究発表ペーパー・要旨(国際会議)

  • Hydrogen peroxide is prerequisite for TNF-alpha production from macrophages communicated with intestinal epithelial cells stimulated with anti-tumor polysaccharides

    M. Mizuno, S. Kawakami

    TAYLOR & FRANCIS LTD, 2006年, FREE RADICAL RESEARCH, 40, S125 - S125, 英語

    研究発表ペーパー・要旨(国際会議)

  • 植物疾病における活性酸素発生と細胞死の病理学

    多田 安臣, 水野 雅史, 姚 楠, 眞山 滋志

    2003年, 化学と生物, 41,542-548, 日本語

    その他

  • Mechanism of TNF-alpha and no production from macrophage stimulated with alkaline-soluble polysaccharides purified from Agaricus blazei Murrill (wade strain 101).

    S Kawakami, M Mizuno

    AMER CHEMICAL SOC, 2002年04月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 223, U39 - U39, 英語

    研究発表ペーパー・要旨(国際会議)

  • Hirofumi Terai, Keiichi Hayashi, Masashi Mizuno, Hironobu Tsuchida

    The rates of carbon dioxide and ethylene production and the levels of 1-aminocyelopropane-1-carboxylic acid (ACC) and polyamines, putrescine and spermidine, in chilling-sensitive water convolvulus and resistant chingensai were measured during storage at constant 5°C and 20°C or with temperature rise from 5°C to 20°C. Water convolvulus stored at constant 5°C or transferred from 5°C to 20°C on day 3 developed chilling injury on day 4, while chingensai showed no chilling injury at any temperature storage. Following temperature rise to 20°C with two periods of storage at 5°C, water convolvulus showed differences between the two in the rates of carbon dioxide and ethylene production and the levels of polyamines, putrescine and spermidine however, chingensai remained essentially the same. These results suggest that chilling stress affected the ethylene biosynthetic pathway and polyamine metabolism in chilling-sensitive water convolvulus but not in resistant chingensai.

    S. Karger AG, 1999年, Food Science and Technology Research, 5 (1), 64 - 68, 英語

    書評論文,書評,文献紹介等

  • HERB WATER-EXTRACTS MARKEDLY SUPPRESS THE MUTAGENICITY OF TRP-P-2

    M NATAKE, K KANAZAWA, M MIZUNO, N UENO, T KOBAYASHI, G DANNO, S MINAMOTO

    JAPAN SOC BIOSCI BIOTECHN AGROCHEM, 1989年05月, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 53 (5), 1423 - 1425, 英語

    記事・総説・解説・論説等(学術雑誌)

書籍等出版物

  • 生体機能を高める乳酸菌・ビフィズス菌の研究開発と応Enterococcus faecalis IC-1によるⅠ型アレルギー抑制効果、腸内細菌叢を標的にした医薬品と保健機能食品の開発

    水野 雅史, 坂根 巌

    共著, 技術情報協会, 2018年09月, 日本語

    学術書

  • βグルカン受容体を介した抗炎症効果、βグルカンの基礎研究と応用・利用の動向、大野尚仁監修

    水野 雅史, 湊 健一郎

    共著, シーエムシー出版, 2018年07月, 日本語

    学術書

  • スマート乳酸菌の新機能

    大戸 信明, 水野 雅史

    共著, (株)食品化学新聞社, 2017年03月, 日本語

    学術書

  • 27節 炎症性腸疾患、第6章 効能ごとの機能性、安全性評価法と製品プロモーションに有用な科学的データの示し方、機能性食品表示への科学的なデータの取り方と表示できる許容範囲

    水野 雅史, 橋本 堂史

    共著, (株)技術情報協会, 2015年04月, 日本語

    学術書

  • 担子菌由来食物繊維と炎症性腸疾患

    水野 雅史

    共著, Functional Food, 2013年04月, 日本語

    学術書

  • レンチナン、きのこの生理活性と機能 河岸洋和監修 pp.128-134

    水野 雅史

    共著, シーエムシー出版, 2005年, 日本語

    学術書

  • きのこ類、栄養・食料学 データハンドブック、日本栄養・食糧学会編 pp260

    水野 雅史

    共著, 同文書院, 2005年, 日本語

    学術書

  • ヒメマツタケ由来多糖による免疫賦活作用に関与する受容体(pp.1-5)

    水野 雅史

    共著, Bio Medical Quick Review Net, 2003年, 日本語

    学術書

  • Tea Extracts Modurate A Glucose Transport System in 3T3- L1 Adipocytes.(Chapter 20, 224-234)

    FURUYASHIKI T, TERASHIMA S, NAGAYASU H, KANEKO A, SAKANE I, DANNO G, MINATO K, KAWAKAMI S, MIZUNO Masashi

    共著, In "Food Factors in Health Promotion and Disease Prevention", 2003年, 英語

    学術書

  • Suppression of Cytochrome P4501A Subfamily in Mouse Liver by Oral Intake of Polysaccharides from Mushrooms, Lentinus edodes and Agaricus blazei.(Chapter 21, 235-248)

    Hitoshi Ashida, Takashi Hashimoto, Yuji Nonaka, Itsuko Fukuda, Kazuki Kanazawa, Gen-ichi Danno, Ken-ichiro Minato, Sachiko Kawakami, Masashi Mizuno

    共著, American Chemical Society, 2003年, 英語

    学術書

  • Stimulation of TNF- and NO production from murine macrophage by water-soluble polysaccharides from Isaria japonica, In Food Factors in Health Promotion and Disease Prevention(pp. 152-162.)

    KAWAKAMI S, MINATO K, IMAMURA T, AIZONO Y, MIZUNO Masashi

    共著, Washington, DC, American Chemical Society, 2003年, 英語

    学術書

  • Preventive Effects of Food Components on Caspase-8- mediated Apoptosis Induced by Dietary Carcinogen, Trp-P-1, in Rat Mononuclear Cells.(Chapter 12, pp.128-140)

    HASHIMOTO T, ITO W, FURUYASHIKI T, SANO T, MINATO K, MIZUNO Masashi, KANAZAWA Kazuki, DANNO G, ASHIDA Hitoshi

    共著, In "Food Factors in Health Promotion and Disease Prevention", 2003年, 英語

    学術書

講演・口頭発表等

  • 加熱処理によるフコイダン分解物の抗アレルギー効果への影響

    森田温子, 水野雅史

    日本農芸化学会2020年度大会, 2020年03月, 日本語

    口頭発表(一般)

  • 幾何選択的なスフィンガジエン類の合成研究

    中村逸揮, 姜 法雄, 水野雅史, 久世雅樹

    日本農芸化学会関西・中部支部2019年度合同大会, 2019年12月, 日本語

    口頭発表(一般)

  • フコイダンが有する抗アレルギー効果の安定性について

    森田温子, 水野雅史

    日本応用糖質科学会平成31年度大会, 2019年09月, 日本語

    口頭発表(一般)

  • The mushroom β-glucan, PCPS, can prevent inflammatory response, an allergic contact dermatitis, induced by DNFB in mice

    KEN-ICHIRO MINATO, AKIHITO OHARA, MASASHI MIZUNO

    30th Annual Conference of the Australasian Society of Clinical Immunology and Allergy, 2019年09月, 英語

  • Structural change of fucoidan extracted from Saccharina japonica by heat treatment and its anti-allergy property

    MASASHI MIZUNO, ATSUKO MORITA, KEN-ICHIRO MINATO

    30th Annual Conference of the Australasian Society of Clinical Immunology and Allergy, 2019年09月, 英語

  • エンテロコッカスIC-1とルテオリンの同時摂取による抗アレルギー効果の増強

    Han Lulu, Takashi Hashimoto, Iwao Sakane, Masashi Mizuno

    日本食品科学工学会第65回大会, 2019年09月, 英語

    口頭発表(一般)

  • エンテロコッカスIC-1とルテオリンの組み合わせによる抗アレルギー効果の相乗的的増強

    HAN LULU, IWAO SAKANE, MASASHI MIZUNO

    2019年08月, 英語

  • 血中TGF-β産生を促すLactobacillus plantarum 22A-3による抗アレルギー効果

    藤井美凪, 大戸信明, 桒原浩誠, 水野雅史

    日本農芸化学会平成31年度大会, 2019年03月, 日本語

  • 食品由来高分子多糖による腸管を介した生理機能

    水野 雅史

    食品開発展2018, 2018年10月, 日本語, 東京ビッグサイト, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • The Polysaccharide from Pleurotus citrinopileatus regulates the differentiation of monocyte toward macrophages

    MINATO KEN-ICHIRO, MIZUNO MASASHI

    International Conference on Pure and Applied Chemistry, Chemistry for a Clean and Healthy Planet, 2018年07月, 英語, Mauritius, 国際会議

    ポスター発表

  • Effects of dietary glucosylceramide from pineapples on type I hypersensitivity

    TAKEMURA AYUMI, OHTO NOBUAKI, KUWAHARA HIROSHIGE, MIZUNO MASASHI

    International Conference on Pure and Applied Chemistry, Chemistry for a Clean and Healthy Planet, 2018年07月, 英語, Mauritius, 国際会議

    ポスター発表

  • パイナップル果実由来グルコシルセラミドのアレルギー抑制効果とLMIR3の関与

    竹村 有裕美, 大戸 信明, 桒原 浩誠, 水野 雅史

    日本農芸化学会関西支部第502回講演会, 2018年02月, 日本語, 京都大学百周年時計台記念館, 国内会議

    口頭発表(一般)

  • 食品に含まれる高分子多糖の機能

    水野 雅史

    第3回日本農芸化学会関西支部産学官連携シンポジウム, 2017年10月, 日本語, 江崎グリコ中央研究所, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 食品中多糖類の腸管上皮細胞を介した炎症抑制機構

    水野 雅史

    共同開催フォーラム第7回食の安全研究ホットトピックス, 2017年09月, 日本語, 東北大学, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Lentinan exerts anti-inflammatory activity by suppression of transfer of tumor necrosis factor receptor 1 to surface on intestinal epithelial cell through Dectin-1 receptor

    MIZUNO MASASHI, MINATO KEN-ICHIRO, HASHIMOTO TAKASHI

    The 9th International Medicinal Mushroom Conference, 2017年09月, 英語, Palermo, Italy, 国際会議

    口頭発表(一般)

  • Immunomodulating effects of the edible mushroom, Pleurotus citrinopileatus, on innate immunocompetent cells such as dendritic cells and macrophage

    MINATO KENーOCHIRO, MIZUNO MASASHI, LAAN C. LISA, OHARA AKIHIRO, VAN DIE IRMA

    The 9th International Medicinal Mushroom Conference, 2017年09月, 英語, Palermo, Italy, 国際会議

    口頭発表(一般)

  • パイナップル由来グルコシルセラミドの皮膚機能改善機構におけるTGF-βのコラーゲン産生とIL-23の関与

    竹村 有裕美, 橋本 堂史, 大戸 信明, 桒原 浩誠, 水野 雅史

    日本食品科学工学会第64回大会, 2017年08月, 日本語, 日本大学, 国内会議

    口頭発表(一般)

  • 抗原感作状態でのF-フコイダンのアレルギー抑制効果

    坂口 香奈, 橋本 堂史, 水野 雅史

    日本農芸化学会関西支部第498回講演会, 2017年02月, 日本語, 京都大学百周年時計台記念館, 国内会議

    口頭発表(一般)

  • カテキンに惹起される内分泌様培養細胞における細胞内カルシウムシグナル伝達経路の解析

    岸田 佳織, 藍原 祥子, 橋本 堂史, 水野 雅史

    日本農芸化学会関西支部第498回講演会, 2017年02月, 日本語, 京都大学百周年時計台記念館, 国内会議

    口頭発表(一般)

  • パイナップル由来グルコシルセラミド経口摂取による皮膚機能改善効果とそ作用機序

    西谷 洋輔, 岡 早紀子, 桑田 崇志, 橋本 堂史, 大戸 信明, 桒原 浩誠, 水野 雅史

    第9回セラミド研究会, 2016年10月, 日本語, 東京ユビキタス協創広場CANVAS, 国内会議

    口頭発表(一般)

  • アリルイソチオシアネートおよびその代謝物の肝がん細胞に及ぼす影響

    中村 紫乃, 橋本 堂史, 網野 紗与, 水野 雅史

    第31回日本香辛料研究会, 2016年10月, 日本語, 長浜勤労者福祉会館, 国内会議

    口頭発表(一般)

  • The elucidation of the mechanism of lentinan-induced inflammatory suppression by visualizimg TNFR1 on the membrane

    SAKAGUCHI KANA, HASHIMOTO TAKASHI, ITO TOSHIKI, SHIRAI YASUHIDE, MIZUNO MASASAHI

    Functional and Medical Foods for Chronic Diseases: Bioactive Compounds and Biomarkers, 2016年09月, 英語, Harvard Medical School, 国際会議

    ポスター発表

  • OVA誘導アレルギーモデルにおけるF-フコイダンの抗アレルギー効果

    坂口 香奈, 谷野 有佳, 橋本 堂史, 水野 雅史

    日本応用糖質科学会平成28年度大会, 2016年09月, 日本語, 学校法人福山大学宮地茂記念館, 国内会議

    口頭発表(一般)

  • レンチナンによって誘導されるTNFR1発現変化の可視化による炎症抑制機構の解明

    坂口 香奈, 橋本 堂史, 伊藤 俊樹, 白井 康仁, 水野 雅史

    日本食品科学工学会第63回大会, 2016年08月, 日本語, 名城大学, 国内会議

    口頭発表(一般)

  • 乳酸菌が産生する菌体外多糖(Extracellular polysaccharides: EPS)の腸管細胞を介した免疫賦活性試験

    岸本 真奈, 水野 雅史, 大澤 朗

    日本農芸化学会平成28年度大会, 2016年03月, 日本語, 札幌, 国内会議

    ポスター発表

  • マラリア原虫Plasmodium berghei感染ICRマウスにおける硫酸化多糖の抗マラリア効果

    嘉正 雄太, 橋本 堂史, 藍原 祥子, 水野 雅史

    日本農芸化学会平成28年度大会, 2016年03月, 日本語, 札幌, 国内会議

    ポスター発表

  • アリルイソチオシアネート代謝物が肝細胞の脂質蓄積に及ぼす影響に関する研究

    網野 紗与, 橋本 堂史, 中村 紫乃, 藍原 祥子, 水野 雅史

    日本農芸化学会平成28年度大会, 2016年03月, 日本語, 札幌, 国内会議

    ポスター発表

  • Oral administration of pineapple glucosylceramide improves skin barrier function by enhancement of collagen production in Hos:HR-1 hairless mice

    岡 早紀子, 橋本 堂史, 大戸 信明, 桒原 浩誠, 水野 雅史

    Global Conference on Life Science and Biological Engineering, 2016年03月, 英語, Kyoto, Serum TGF-β levels of the mice fed the HR-AD diet significantly reduced to about half of the standard diet. However, it recovered to almost the same level as the standard diet in the P-GlcCer group. Collagen contents of skin, which was measured as hydroxyproline, significantly reduced in the mice fed the HR-AD diet compared with the standard diet. On the other hand, P-GlcCer su, 国際会議

    ポスター発表

  • 食品に含まれる多糖類の機能性

    水野 雅史

    日本応用糖質科学会 第40回近畿支部会, 2015年12月, 日本語, 大阪, レンチナンおよびフコイダンの生理活性に関する研究内容を述べた。, 国内会議

    口頭発表(基調)

  • The anti-allergic effects of enterococcus faecalis through toll-like receptor 2

    山下 紗枝, 横山 友紀, 橋本 堂史, 藍原 祥子, 水野 雅史

    Proceedings of the 19th international conference, 2015年11月, 英語, Kobe, 国際会議

    ポスター発表

  • Oral intake of F-fucoidan from Laminaria Japonica prevents type I allergy through secretion of galectin-9

    谷野 有佳, 橋本 堂史, 藍原 祥子, 尾島 孝男, 水野 雅史

    Proceedings of the 19th international conference, 2015年11月, 日本語, Kobe University, 国際会議

    ポスター発表

  • Anti-inflammatory property of Lactobacillus plantarum 22A-3 through small intestinal epitherial cells

    水野 雅史

    The 19th International Conference, Kobe Univrsity, 2015年11月, 英語, Kobe University, Background: The intestinal bacterial flora contributes significantly to the pathogenesis of inflammatory bowel disease (IBD) along with mucosal immune dysregulation and genetic factors. Dysbiosis in the intestinal microflora is associated with the development of IBD. Extensive research is focused on modifying the intestinal microbiota with probiotic bacteria to reduce excessive, 国際会議

    口頭発表(招待・特別)

  • 生酛乳酸菌のバイオジェニック効果に着目した米乳酸発酵飲料の開発

    高橋 俊成, 増田 康之, 吉田 和利, 水野 雅史

    第67回日本生物工学会大会, 2015年10月, 日本語, 鹿児島, バイオジェニックス乳酸菌として、死滅菌体でも免疫調節作用の高い乳酸菌株を選抜することにし、マクロファージ様細胞株、マウス、培養細胞の共培養系を利用した腸管免疫モデルなどを用いて免疫調節作用の高い乳酸菌としてLactobacillus sakei LK-117株を選抜した。この乳酸菌体をアトピー性皮膚炎を発症するマウスに継続投与したところ、アトピー性皮膚炎の炎症が抑制された。一方、発酵基質に用いるプレバイオティクス効果の高い米品種として、難消化性デンプン含量が高い米品種「ホシニシキ」を選抜した。このホシニシキと免疫調節機能を有するLK-117株を組み合わせ、共同研究先の菊正宗酒造株式会社が持つ生酛製造技術を応用することにより、高密度に乳酸菌を培養することに成功し、バイオジェニックス効果とプレバイオティクス効果を併せ持つ乳性分を含まない総合的機能性飲料の, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • マコンブ由来F-フコイダンによる新規Ⅰ型アレルギー抑制機構

    谷野 有佳, 橋本 堂史, 藍原 祥子, 尾島 孝男, 水野 雅史

    日本応用糖質科学会平成27年度大会, 2015年09月, 日本語, 奈良, 【目的】これまでに我々はマコンブに含まれるF-フコイダンがⅠ型アレルギー抑制効果を有しており、その効果にガレクチン9が関与していることを明らかにしてきた。ガレクチン9はβガラクトシドを認識するレクチンの一種で、近年IgEと結合することでIgEの肥満細胞への結合を阻害することが報告されている。今回、我々は実際にF-フコイダンによりガレクチン9が血中で増加してⅠ型アレルギー症状を抑制しているのかを検討した。 【方法】F-フコイダン(200 μg/日)を4日間胃内強制投与した。抗原特異的IgEを尾静脈から投与し、30分後に耳介厚を測定、抗原を耳介に塗布した。その2時間後に再び耳介厚を測定し、抗原刺激前後の耳介厚の差を浮腫として表した。F-フコイダンにより誘導されたガレクチン9の関与を調べるため、ガレクチン9のアンタゴニストであるラクトースを抗原特異的IgE, 国内会議

    口頭発表(一般)

  • Anti-inflammatory property of orally administered lentinan through intestinal immunity in ileum in DSS-induced colitis mice

    水野 雅史, 大浦 圭吾, 橋本 堂史

    The 8th International Medicinal Mushroom Conference, 2015年08月, 英語, Manizales, Colombia, Ulcerative colitis (UC) and Crohn’s disease (CD) are pathologically distinct disorders but are commonly refer to as inflammatory bowel disease (IBD). Both conditions show a chronic and relapsed inflammation of the intestine, in which intestinal epithelial cells and macrophages secrete large amounts of chemokines and pro-inflammatory cytokines. Current treatment of IBD relies ma, 国際会議

    口頭発表(一般)

  • 食品由来成分による消化管ホルモン分泌に関する細胞内メカニズム

    藍原 祥子, 宋 苑昤, 岸田 佳織, 水野 雅史

    第15回AOB研究会, 2015年07月, 日本語, 大阪, 国内会議

    口頭発表(一般)

  • Lactobacillus plantarum 22A-3 株はTLR2 を介して小腸粘膜固有層で制御性T 細胞を誘 導し炎症を抑制する

    林 多恵子, 橋本 堂史, 水野 雅史

    日本乳酸菌学会2015年度大会, 2015年07月, 日本語, 和洋女子大学, 国内会議

    口頭発表(一般)

  • 鉄欠乏マウスにおける味覚嗜好性の変化

    宮本 岳, 藍原 祥子, 橋本 堂史, 水野 雅史

    日本農芸化学会平成27年度大会, 2015年03月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • ルテオリンの炎症抑制効果に対するフラボノイド同時摂取の影響

    山下 紗枝, 藍原 祥子, 橋本 堂史, 水野 雅史

    日本農芸化学会平成27年度大会, 2015年03月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • マコンブ由来F-フコイダンのⅠ型アレルギー抑制効果に対するガレクチン9の関与

    谷野 有佳, 水野 雅史, 橋本 堂史

    日本農芸化学会平成27年度大会, 2015年03月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 237.マコンブ由来F-フコイダンのⅠ型アレルギー抑制効果に対するガレクチン9の関与

    谷野 有佳, 藍原 祥子, 橋本 堂史, 尾島 孝男, 水野 雅史

    日本農芸化学会平成27年度大会, 2015年03月, 日本語, 岡山大学, 国内会議

    口頭発表(一般)

  • 腸管セレトニン産生細胞におけるTRPチャンネルの発現解析

    滝沢 彰良, 藍原 祥子, 橋本 堂史, 水野 雅史

    日本農芸化学会関西支部第488回講演会, 2015年01月, 日本語, 京都大学楽友会館, 国内会議

    口頭発表(一般)

  • 機能性多糖レンチナンは小腸上皮細胞を介して免疫応答を制御する

    大浦 圭吾, 橋本 堂史, 水野 雅史

    日本農芸化学会関西支部第488回講演会, 2015年01月, 日本語, 京都大学楽友会館, 国内会議

    口頭発表(一般)

  • エピガロカテキンガレートはGLP-1を介して血糖値を調整する

    小田 あかね, 藍原 祥子, 橋本 堂史, 水野 雅史

    日本農芸化学会関西支部第488回講演会, 2015年01月, 日本語, 京都大学楽友会館, 国内会議

    口頭発表(一般)

  • Lactobacillus delbrueckiiが産生する菌体外多糖の免疫賦活活性に関する研究

    岸本 真奈, 野本 竜平, 水野 雅史, 大澤 朗

    日本農芸化学会関西支部第487回講演会, 2014年12月, 日本語, 神戸大学, 国内会議

    口頭発表(一般)

  • 6-メチルスルフィニルヘキシルイソチオシアネートの細胞周期開始抑制機序に関する研究

    小寺 裕貴, 橋本 堂史, 石村 麻耶, 藍原 祥子, 金沢 和樹, 三宅 秀芳, 吉田 優, 水野 雅史

    日本農芸化学会 関西支部 支部例会(第487回講演会), 2014年12月, 日本語, 神戸大学農学部C101教室(神戸), 国内会議

    口頭発表(一般)

  • Synergistic anti-inflammatory activity of luteolin and some polyphenols in in vitro co-culture system composed of Caco-2 and RAW264.7 cells

    Masashi Mizuno, Takashi Hashimoto

    The Society for Free Radical Biology and Medicine’s 21th Annual Meeting (SFRBM 2014), 2014年11月, 日本語, Sheraton Seattle, Seattle, WA, USA, 国際会議

    ポスター発表

  • SHRSP5/Dmcrラットにおける非アルコール性脂肪性肝疾患に対するクロロゲン酸の抑制効果

    宮崎 泰一, 橋本 堂史, 藍原 祥子, 北森 一哉, 水野 雅史

    第19回 日本フードファクター学会学術集会, 2014年11月, 日本語, 鹿児島大学郡元キャンパス(鹿児島), 国内会議

    口頭発表(一般)

  • 小腸を介した食品因子の抗アレルギー効果を評価する新規評価法の有用性

    横山 友紀, 橋本 堂史, 水野 雅史

    日本食品免疫学会 第10回学術大会, 2014年10月, 日本語, 東京大学伊藤国際学術研究センター(東京), 国内会議

    口頭発表(一般)

  • パイナップル由来グルコシルセラミドが乾燥肌モデルマウスの免疫系に及ぼす影響

    桑田 祟志, 桒原 浩誠, 大戸 信明, 橋本 堂史, 水野 雅史

    第7回 セラミド研究会学術集会, 2014年10月, 日本語, 東京ユビキタス協創広場CANVAS(東京), 国内会議

    口頭発表(一般)

  • Lactobacillus plantarum 22A-3によるTGF-β産生促進と制御性T細胞の誘導効果

    Jarukan Lamubol, 橋本 堂史, 林 多恵子, 西谷 洋輔, 水野 雅史

    日本食品免疫学会 第10回学術大会, 2014年10月, 日本語, 東京大学伊藤国際学術研究センター(東京), 国内会議

    口頭発表(一般)

  • Lactobacillus plantarum 22A-3 によるTGF-β1産生促進と制御性T細胞の誘導効果

    Jarukan Lamubol, 水野 雅史

    日本食品免疫学会第10回大会, 2014年10月, 日本語, 東京大学, 国内会議

    口頭発表(一般)

  • 食品由来多糖類によるナイーブT細胞分化の制御

    大浦 圭吾, 橋本 堂史, 水野 雅史

    日本応用糖質科学会 平成26年度大会, 2014年09月, 日本語, 朱鷺メッセ新潟コンベンションセンター(新潟), 国内会議

    口頭発表(一般)

  • 食品に含まれる機能成分による腸管免疫制御機構

    水野 雅史

    日本化粧品技術者会第200回講演会, 2014年09月, 日本語, 日本化粧品技術者会, 大阪薬業年金会館, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 抗炎症効果を有するルテオリンの吸収促進物質の探索

    山下 紗枝, 橋本 堂史, 藍原 祥子, 水野 雅史

    日本食品科学工学会 第61回大会, 2014年08月, 日本語, 中村学園大学(福岡), 国内会議

    口頭発表(一般)

  • マコンブ由来F-フコイダンの抗アレルギー効果

    谷野 有佳, 藍原 祥子, 橋本 堂史, 尾島 孝男, 水野 雅史

    日本食品科学工学会 第61回大会, 2014年08月, 日本語, 中村学園大学(福岡), 国内会議

    口頭発表(一般)

  • シイタケは腸を健全に保ちます

    水野 雅史

    吉備国際大学「健康増進」市民シンポジウム, 2014年08月, 日本語, 南あわじ市, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • Lactobacillus plantarum 22A-3のアレルギー抑制効果について

    林 多恵子, 西谷 洋輔, 桒原 浩誠, 水野 雅史

    日本乳酸菌学会, 2014年07月, 日本語, 広島, 国内会議

    口頭発表(一般)

  • 消化管ホルモンGLP-1を介したAOBの生理効果

    小田 あかね, 宋 苑昤, 藍原 祥子, 金沢 和樹, 橋本 堂史, 水野 雅史

    第14回 AOB研究会, 2014年06月, 日本語, ホテル日航福岡(福岡), 国内会議

    口頭発表(一般)

  • Lactobacillus plantarum 22A-3の腸管上皮細胞におけるTGF-β1産生誘導効果

    Jarukan Lamubol, 橋本 堂史, 林 多恵子, 西谷 洋輔, 水野 雅史

    第18回 腸内細菌学会, 2014年06月, 日本語, 東京大学 伊藤国際学術研究センター(東京), 国内会議

    口頭発表(一般)

  • ルチン経口投与によるマウス薬物代謝第Ⅱ相酵素に及ぼす影響

    岩見 志歩, 橋本 堂史, 藍原 祥子, 水野 雅史

    第68回 日本栄養・食糧学会大会, 2014年05月, 日本語, 明治大学生田キャンパス(神奈川県川崎市), 国内会議

    口頭発表(一般)

  • Lactobacillus delbrueckiiが産生する菌体外多糖の免疫賦活活性に関する研究

    岸本 真奈, 野本 竜平, 水野 雅史, 大澤 朗

    2014年度日本乳酸菌学会泊まり込みセミナー, 2014年05月, 日本語, 那須オオシマフォーラム, 国内会議

    口頭発表(一般)

  • 皮膚機能を改善するパイナップル果実由来グルコシルセラミドによる小腸のIL-23 mRNA発現の亢進

    桑田 崇志, 野嶋 潤, 大戸 信明, 桑原 浩誠, 橋本 堂史, 水野 雅史

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 明治大学生田キャンパス(神奈川県川崎市), 国内会議

    口頭発表(一般)

  • 腸管上皮細胞からのTGF-β1産生誘導を伴うLactobacillus plantarum 22A-3 の腸管炎症抑制効果

    ランウボン ジャルカン, 橋本 堂史, 林 多恵子, 西谷 洋輔, 水野 雅史

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 明治大学生田キャンパス(神奈川県川崎市), 国内会議

    口頭発表(一般)

  • タマネギ外皮抽出物のStaphylococcus aureusに対する抗菌効果に関する研究

    黄 達, 橋本 堂史, 藍原 祥子, 金沢 和樹, 水野 雅史

    日本農芸化学会2014年度大会, 2014年03月, 日本語, 明治大学生田キャンパス(神奈川県川崎市), 国内会議

    口頭発表(一般)

  • 234.機能性多糖レンチナンは小腸上皮細胞を介して免疫応答を制御する

    大浦 圭吾, 橋本 堂史, 水野 雅史

    日本農芸化学会関西支部第488回講演会, 2014年01月, 英語, 京都大学楽友会館, 国内会議

    口頭発表(一般)

  • 経口摂取を想定した抗アレルギー効果を有する食品因子探索法の確立

    横山 友紀, 橋本 堂史, 水野 雅史

    日本農芸化学会 関西支部 支部例会(第482回講演会), 2013年12月, 日本語, 神戸大学農学部C101教室(神戸), 国内会議

    口頭発表(一般)

  • フコキサンチノールが誘導するアポトーシスはアクチン重合を伴う

    大原 佳樹, 橋本 堂史, 藍原 祥子, 金沢 和樹, 水野 雅史

    日本農芸化学会 関西支部 支部例会(第482回講演会), 2013年12月, 日本語, 神戸大学農学部C101教室(神戸), 国内会議

    口頭発表(一般)

  • Lactobacillus plantarum 22A-3による腸管炎症抑制効果の作用機序の解明

    Jarukan Lamubol, 橋本 堂史, 林 多恵子, 奥田 洋, 水野 雅史

    日本農芸化学会 関西支部 支部例会(第482回講演会), 2013年12月, 日本語, 神戸大学農学部C101教室(神戸), 国内会議

    口頭発表(一般)

  • レンチナンによる腸炎抑制を引き起こす小腸上皮細胞様受容体について

    雉子谷 百合江, 西谷 洋輔, 橋本 堂史, 水野 雅史

    日本応用糖質科学会 平成25年度大会, 2013年09月, 日本語, 鹿児島大学(鹿児島), 国内会議

    口頭発表(一般)

  • 腸上皮様培養細胞の消化管ホルモン分泌に対するAOBの効果

    宋 苑昤, 藍原 祥子, 橋本 堂史, 金沢 和樹, 水野 雅史

    第13回 AOB研究会, 2013年07月, 日本語, 東京, 国内会議

    口頭発表(一般)

  • 食品因子の抗アレルギー効果を評価する新規評価法の有効性

    横山 友紀, 橋本 堂史, 水野 雅史

    日本農芸化学会2014年度大会, 2013年03月, 日本語, 明治大学生田キャンパス(神奈川県川崎市), 国内会議

    口頭発表(一般)

  • パン酵母由来β-グルカン(BBG)のマクロファージにおける抗炎症作用

    保田 倫子, 許 小娟, 水野 雅史, 芦田 均

    日本農芸化学会2013年度大会, 2013年, 日本語, 仙台, 国内会議

    口頭発表(一般)

  • マウス黒色腫細胞におけるメラニン産生に対するAOBの抑制効果

    藍原 祥子, 松代 小春, 橋本 堂史, 水野 雅史, 金沢 和樹

    第12回AOB研究会, 2012年06月, 日本語, 京都, 国内会議

    口頭発表(一般)

  • β-Glucan from Saccharomyces cerevisiae reduces lipopolysaccharide-induced inflammatory responses in RAW264.7 Macrophages

    YASUDA Michiko, XU Xiaojuan, MIZUNO Masashi, ASHIDA Hitoshi

    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT 2012 Nagoya), 2012年, 英語, 名古屋, 国際会議

    口頭発表(一般)

  • パン酵母由来β-グルカンのTLR2およびTLR4を介した炎症反応に対する抑制作用

    保田 倫子, 許 小娟, 水野 雅史, 芦田 均

    第17回日本フードファクター学会学術集会・第9回日本カテキン学会総会合同大会, 2012年, 日本語, 静岡, 国内会議

    口頭発表(一般)

  • パン酵母β‐グルカンのマクロファージにおける炎症抑制効果

    保田 倫子, 許 小娟, 水野 雅史, 芦田 均

    第65回日本栄養・食糧学大会, 2011年, 日本語, 国内会議

    口頭発表(一般)

  • パン酵母由来β-グルカンの炎症抑制作用

    許 小娟, 保田 倫子, 水野 雅史, 芦田 均

    第15回日本フードファクター学会学術集会, 2010年, 日本語, 国内会議

    口頭発表(一般)

  • ピタヤの水溶性粘性画分の物理的特性と機能性

    橋爪 佐依, 坂本 拓也, 米本 仁巳, 水野 雅史, 角田 万里子, 野村 啓一

    園芸学会平成21年度春季大会, 2009年03月, 日本語, 明治大学, 国内会議

    ポスター発表

  • キノコ由来多糖による免疫細胞活性化機構と小腸細胞の関わり

    水野 雅史

    平成17年度第2回バイオ産業創成研究会, 2005年11月, 日本語, 社団法人京都経営・技術研究会, 京都, 国内会議

    口頭発表(一般)

  • 病原菌感染時における非光合成器官内での防御応答について

    前田 由香, 川上 佐知子, 水野 雅史

    日本農芸化学会平成17年度大会, 2005年03月, 日本語, 日本農芸化学会, 北海道, 国内会議

    口頭発表(一般)

  • 小腸上皮細胞Caco2細胞とマクロファージ様細胞RAW264.7 細胞との共培養系における免疫賦機構の解析

    中尾 太, 川上 佐知子, 水野 雅史

    日本農芸化学会平成17年度大会, 2005年03月, 日本語, 日本農芸化学会, 北海道, 国内会議

    口頭発表(一般)

  • レンチナン分解活性を持つexg2遺伝子の解析

    坂本 裕一, 湊 健一郎, 川上 佐知子, 水野 雅史, 佐藤 利次

    日本農芸化学会平成17年度大会, 2005年03月, 日本語, 日本農芸化学会, 北海道, 国内会議

    口頭発表(一般)

  • Polysaccharides purified from Agaricus blazei Murrill stimulate macrophages through Toll-like receptor 4 and do so more strongly through intestinal epithelial cells,

    KAWAKAMI S, MIZUNO Masashi

    Focus on Fungal Infections 15, 2005年, 英語, 未記入, Miami USA, 国際会議

    口頭発表(一般)

  • Inhibitory effects of extracts from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae) on immediate type allergy induced compound 48/80 in ICR mice,

    MIZUNO Masashi, KAWAKAMI S, CHIDA Y

    The Third International Medicianl Mushroom Conference, 2005年, 英語, 未記入, Port Townsend, USA., 国際会議

    口頭発表(一般)

  • Defense mechanism against active oxygen species induced by beta-glucan elicitor in potato suspension culture,

    KAWAKAMI S, MIZUNO Masashi, TADA Y, MAYAMA Shigeyuki

    2nd Asian Conference on Plant Pathology, 2005年, 英語, 未記入, Singapore, 国際会議

    口頭発表(一般)

  • シイタケexo-グルカナーゼ遺伝子の単離

    坂本 裕一, 湊 健一郎, 川上 佐知子, 水野 雅史, 佐藤 利次

    日本農芸化学会平成16年度大会, 2004年03月, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • β-グルカンエリシターによって誘導される細胞死はalternative oxidaseとcatalaseによって制御される

    打井 公隆, 多田 安臣, 川上 佐知子, 水野 雅史

    日本農芸化学会平成16年度大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • レンチナンによる免疫賦活化は小腸上皮細胞を介した過酸化水素発生をともない起こる

    水野 雅史, 川上 佐知子, 芦田 均

    第63回日本癌学会総会, 2004年, 日本語, 日本癌学会, 博多, 国内会議

    口頭発表(一般)

  • シイタケレンチナンによる薬物代謝酵素発現抑制効果

    芦田 均, 岡本 隆志, 小土井 理恵, 柳 由貴子, 川上 佐知子, 水野 雅史

    2004年度日本農芸化学会大会, 2004年, 日本語, 日本農芸化学会, 広島, 国内会議

    口頭発表(一般)

  • キノコ由来多糖によるマクロファージ活性化機構

    水野 雅史

    食品開発展2004, 2004年, 日本語, 未記入, 東京, 国内会議

    口頭発表(一般)

  • Lentinan is degraded during preservation.

    SAKAMOTO Yuuichi, KAWAKAMI Sachiko, KAWATA M, IRIE T, MIZUNO Masashi, SATO T

    ⅩⅤⅠth International Congress on the Science and Cultivation of Edible and Medicinal Fungi., 2004年, 英語, 未記入, Miami, USA, 国際会議

    口頭発表(一般)

  • Immunomodulating polysaccharide from Agaricus blazei Murrill was recognized through Toll-like receptor 4.

    MIZUNO Masashi, KAWAKAMI Sachiko

    5th International Conference on Systems Biology., 2004年, 英語, 未記入, Heidelberg, Germany, 国際会議

    口頭発表(一般)

  • Hydrogen peroxide is a trigger of TNF-□ production in co-culture with macrophages and intestinal epithelial cells stimulated with lentinan.

    KAWAKAMI Sachiko, MIZUNO Masashi

    Oxygen club of California, 2004年, 英語, 未記入, Santa Barbara, USA., 国際会議

    口頭発表(一般)

  • キノコ由来高分子多糖によるマクロファージ活性化

    水野 雅史

    1st International Symposium of Global Environment and Human Well-being, 2003年, 日本語, 未記入, Kofu, Japan, 国際会議

    口頭発表(一般)

  • きのこ熱水抽出画分によるマルコファージ活性化機構と活性酸素の関与

    川上 佐知子, 芦田 均, 水野 雅史

    日本農芸化学会平成15年度大会, 2003年, 日本語, 日本農芸化学会, 東京, 国内会議

    口頭発表(一般)

  • きのこを用いた紫イモからの健康・機能性アルコール飲料の製造

    佐々木 尚美, 松井 徳光, 水野 雅史, 福田 祥子, 友田 智美, 大杉 匡弘

    日本農芸化学会平成15年度大会, 2003年, 日本語, 日本農芸化学会, 東京, 国内会議

    口頭発表(一般)

  • アリール炭化水素受容体の活性化を測定するELISA の構築とこれを用いた食品成分のダイオキシン毒性抑制効果の評価

    福田 伊津子, 西海 信, 畑中 豊, 水野 雅史, 芦田 均

    第62回日本癌学会総会, 2003年, 日本語, 日本癌学会, 名古屋国際会議場, 国内会議

    口頭発表(一般)

  • Macrophages stimulated with polysaccharide purified from Agaricus brasiliensis enhance mRNA expression of Th1 cytokine including IL-12 and 18

    MIZUNO Masashi, KAWAKAMI S

    9th Congress of the European Confederation of Medical Mycology, Amsterdam, 2003年, 英語, 未記入, The Netherlands, 国際会議

    口頭発表(一般)

  • Changes of lentinan contents and its physiological activity in Lentinus edodes stored under controlled atmosphere

    KAWAKAMI S, MINATO K, TOKIMOTO K, MIZUNO Masashi

    9th Congress of the European Confederation of Medical Mycology, Amsterdam, 2003年, 英語, 未記入, The Netherlands, 国際会議

    口頭発表(一般)

所属学協会

  • American Chemical Sciety

  • 日本きのこ学会

  • 日本フードファクター学会

  • 日本食品科学工学会

  • 日本癌学会

  • 日本農芸化学会

共同研究・競争的資金等の研究課題