研究者紹介システム

森 直樹
モリ ナオキ
大学院農学研究科 生命機能科学専攻
教授
農学関係
Last Updated :2021/05/08

研究者情報

所属

  • 【主配置】

    大学院農学研究科 生命機能科学専攻
  • 【配置】

    農学部 生命機能科学科

学位

  • 博士(農学), 京都大学

授業科目

ミニ講義

ジャンル

  • 環境・交通 / 農業

コメントテーマ

  • 植物遺伝
  • 栽培植物進化
  • インド在来コムギ
  • 酒米のルーツ
  • 山田錦

研究活動

研究キーワード

  • 栽培植物進化
  • 遺伝資源
  • 植物遺伝

研究分野

  • ライフサイエンス / 進化生物学
  • ライフサイエンス / 遺伝学
  • 環境・農学 / 生物資源保全学
  • 環境・農学 / ランドスケープ科学
  • 環境・農学 / 環境農学
  • 環境・農学 / 遺伝育種科学

論文

  • Yoshihiro Matsuoka, Naoki Mori

    Wiley, 2020年12月, Ecology and Evolution, 10 (24), 13926 - 13937, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Natsumi Ito, Naoki Mori, Naohiko T. Miyashita

    Genetics Society of Japan, 2020年10月01日, Genes & Genetic Systems, 95 (5), 249 - 268, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

    The term ‘plasmon’ is used to indicate the whole cytoplasmic genetic system, whereas ‘genome’ refers to the whole nuclear genetic system. Although maternal inheritance of the plasmon is well documented in Angiosperms, its genetic autonomy from the coexisting nuclear genome still awaits critical examination. We aimed to test this autonomy in two related studies: one was to deter

    the National Academy of Sciences of the United States of America, 2019年02月, Proceedings of the National Academy of Sciences of the United States of America, 116 (8), 3082 - 3090, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Evaluation of grain dimension and weight using backcross recombinant inbred lines between wild and domesticated emmer wheat

    Miyazakki Yuki, Ngoc Pham M, Liberatore, Katie L, Kianian, Shahryar F, Vladutu, Cristian I, Mori Naoki

    Emmer wheat (Triticum turgidum ssp. dicoccum) represents the primitive situation in the domestication of AABB tetraploid wheat. As one of the earliest domesticated grain species, it was a principal crop in the development and spread of Neolithic agriculture in the Old World. Grain weight and dimension (size
    and shape) have been major targets of selection since the beginning of

    近畿作物育種研究会, 2017年11月, Journal of Crop Research, 62, 31 - 36, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takako Shizuka, Naoki Mori, Hakan Ozkan, Shoji Ohta

    Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the progenitor of cultivated emmer wheat (ssp. dicoccum) and durum wheat (ssp. turgidum conv. durum). Because of its full interfertility with domesticated emmer wheat, this wild species can serve as one of the most important genetic resources to improve durum as well as bread wheat. To elucidate the magnitude of genetic variation within a population of wild emmer wheat, variation of chloroplast DNA was investigated using 91 plants, in total, collected from two natural habitats in southern Turkey. Allelic variation at 24 microsatellite loci in the chloroplast genome was investigated using these samples. Allelic variations were observed at 15 microsatellite loci. The number of alleles per locus was the same in the two populations, ranging from 1 to 4 with an average of 2.17. The estimated diversity indices (H) were also very close ranging from 0.00 to 0.70 with an average of 0.28 and 0.29 for the two populations. Based on the observed allelic variation at all chloroplast microsatellite loci, a total of 23 chloroplast haplotypes (plastotypes) were identified. Only two plastotypes were shared in common between the two natural populations, indicating that the two populations are highly differentiated. Furthermore, uneven micro-geographic distribution of plastotypes was found within each population, suggesting limited rate of migration (seeds dispersal rate) in this species. Our study demonstrated the presence of a high level of genetic diversity between and within highly structured populations of wild emmer wheat in southern Turkey.

    TAYLOR & FRANCIS LTD, 2015年05月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 29 (3), 423 - 430, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takako Shizuka, Naoki Mori, Hakan Ozkan, Shoji Ohta

    Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the progenitor of cultivated emmer wheat (ssp. dicoccum) and durum wheat (ssp. turgidum conv. durum). Because of its full interfertility with domesticated emmer wheat, this wild species can serve as one of the most important genetic resources to improve durum as well as bread wheat. To elucidate the magnitude of genetic variation within a population of wild emmer wheat, variation of chloroplast DNA was investigated using 91 plants, in total, collected from two natural habitats in southern Turkey. Allelic variation at 24 microsatellite loci in the chloroplast genome was investigated using these samples. Allelic variations were observed at 15 microsatellite loci. The number of alleles per locus was the same in the two populations, ranging from 1 to 4 with an average of 2.17. The estimated diversity indices (H) were also very close ranging from 0.00 to 0.70 with an average of 0.28 and 0.29 for the two populations. Based on the observed allelic variation at all chloroplast microsatellite loci, a total of 23 chloroplast haplotypes (plastotypes) were identified. Only two plastotypes were shared in common between the two natural populations, indicating that the two populations are highly differentiated. Furthermore, uneven micro-geographic distribution of plastotypes was found within each population, suggesting limited rate of migration (seeds dispersal rate) in this species. Our study demonstrated the presence of a high level of genetic diversity between and within highly structured populations of wild emmer wheat in southern Turkey.

    TAYLOR & FRANCIS LTD, 2015年05月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 29 (3), 423 - 430, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Aloka Lanka Ranawake, Oliver Escano Manangkil, Shinya Yoshida, Takashi Ishii, Naoki Mori, Chiharu Nakamura

    Cold tolerance is an important breeding target in rice production. We studied quantitative trait loci (QTLs) controlling cold tolerance at germination (CTG) and early seedling (CTS) stages, using recombinant inbred lines derived from a japonica x indica cross. CTG was evaluated based on the percentage rate of germination at 15 degrees C for 12 days after pre-incubation of imbibed seeds at 20 degrees C for 2 days. For CTS, seven-day-old seedlings grown at 25 degrees C were subjected to two consecutive periods of three-day cold stress at 4 degrees C with an intervening eight-day recovery at 25 degrees C. CTS evaluation was according to an arbitrary five-point rating system at the fifth day of recovery after each stress period. No correlations were found between CTG and CTS, while a weak correlation was detected between CTS after the first and second stress. By the composite interval mapping, five QTLs for CTG explaining 5.7%-9.3% of the total phenotypic variance (PVE) and nine for CTS with PVE of 5.8%-35.6% were detected. Only one of these QTLs was common, indicating growth-stage specificity of cold tolerance. Four of the five QTLs after the second cold stress were different from the ones after the first cold stress. Analysis of variance test showed significant interactions between alleles at the QTL sites and the two stress conditions with respect to the mean CTS scores. A possible involvement of cold acclimation and usefulness of japonica germplasms in breeding for cold tolerance in indica rice was discussed.

    TAYLOR & FRANCIS LTD, 2014年11月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 28 (6), 989 - 998, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Genetic effect of the Aegilops caudata plasmon on the manifestation of the Ae. cylindrica genome

    Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

    In the course of reconstructing Aegilops caudata from its own genome (CC) and its plasmon, which had passed half a century in common wheat (genome AABBDD), we produced alloplasmic Ae. cylindrica (genome CCDD) with the plasmon of Ae. caudata. This line, designated (caudata)-CCDD, was found to express male sterility in its second substitution backcross generation (SB2) of (caudata)-AABBCCDD pollinated three times with the Ae. cylindrica pollen. We repeatedly backcrossed these SB2 plants with the Ae. cylindrica pollen until the SB5 generation, and SB5F2 progeny were produced by self-pollination of the SB5 plants. Thirteen morphological and physiological characters, including pollen and seed fertilities, of the (caudata)-CCDD SB5F2 were compared with those of the euplasmic Ae. cylindrica. The results indicated that the male sterility expressed by (eaudata)-CCDD was due to genetic incompatibility between the Ae. cylindrica genome and Ae. caudata plasmon that did not affect any other characters of Ae. cylindrica. Also, we report that the genome integrity functions in keeping the univalent transmission rate high.

    GENETICS SOC JAPAN, 2014年10月, GENES & GENETIC SYSTEMS, 89 (5), 195 - 202, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naoki Mori, Shoji Ohta, Hajime Chiba, Toshiya Takagi, Yukiko Niimi, Vasant Shinde, Mukund D. Kajale, Toshiki Osada

    Indian dwarf wheat (Triticum aestivum L. ssp. sphaerococcum (Perc.) Mac Key, synonym: T. sphaerococcum Perc.) is endemic to southern Pakistan and northwestern India. It was one of the main winter crops grown by ancient Indian cultures. However, it disappeared from the record during the early twentieth century, especially after the Green Revolution brought modern wheat varieties into India and Pakistan. Whether or not Indian dwarf wheat is presently cultivated has been unclear. Here we report on the rediscovery of the cultivation of this wheat in northern Karnataka and southern Maharashtra in India. Molecular genetic analysis of the chloroplast DNA of the two specimens collected at location 3 revealed that both samples have a unique haplotype that is specific to Indian dwarf wheat. We found this wheat at three locations in 2010, but at only one of the three locations in 2011. Therefore, the future survival of this subspecies is uncertain. Further ethnobotanical research is urgently needed to conserve this unique genetic resource for the future. © 2013 Springer Science+Business Media Dordrecht.

    Springer, 2013年08月, Genetic Resources and Crop Evolution, 60 (6), 1771 - 1775, 英語

    [査読有り]

    研究論文(学術雑誌)

  • O. E. Manangkil, H. T. T. Vu, N. Mori, S. Yoshida, C. Nakamura

    Submergence-induced suppression of seedling vigor is a serious constraint particularly in the direct seeding rice cultivation system. To identify quantitative trait loci (QTLs) associated with seedling vigor in rice under submergence, a mapping population of 98 Backcross Inbred Lines derived from a cross of Nipponbare/Kasalath//Nipponbare was used. Phenotypic evaluation of seedling vigor under submergence was based on shoot length (SHL), root length (RTL) and shoot fresh weight (SFW) using a test tube bioassay method. Thirty-two putative QTLs were detected among which 7 were for SHL, 11 for RTL and 14 for SFW. Phenotypic evaluation was also made of the parental lines and a set of 54 chromosome segment substitution lines in which Nipponbare segments were substituted for by their homologous Kasalath segments covering the entire rice genome. Two QTLs with more than 10 % contribution to the total phenotypic variance were verified for SHL, and at least one for RTL and six for SFW on chromosomes 1, 3, 4, 6 and 7 at the 1 % significance level. Among these, all but two showed reductions in one, two or all three traits. Our present and previous results suggest that the Nipponbare genome has a potential to improve seedling vigor under submergence and that japonica germplasms can be used to breed for this important trait in indica rice.

    SPRINGER, 2013年07月, EUPHYTICA, 192 (1), 63 - 75, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Phan Thi Thanh, Cristian Ioan Vladutu, Shahryar F. Kianian, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Naoki Mori

    Emmer wheat (Triticum turgidum ssp. dicoccum) was a principal crop in the development and spread of Neolithic agriculture in the Old World It represents the primitive situation in the domestication of AABB tetraploid wheat. To understand the genetic modifications underlying the early stage of tetraploid wheat domestication, QTL analysis was carried out using 144 F-2 plants derived from a cross between the domesticated emmer wheat and the wild emmer wheat (T turgidum ssp. dicoccoides). To our knowledge, this is the first report of molecular linkage map and QTL analysis of the domestication-related characters in emmer wheat. The linkage map with a total length of 2849.8 cM was constructed using 227 microsatellite (SSR) markers. Chromosomal location and effect of QTLs were estimated for ten domestication-related traits including whole plant and spike characteristics. Seventeen QTLs were detected on chromosomes 1B, 2A, 2B, 3A, 3B, 4A, 5B, and 7B. Two regions on chromosomes 2A and 3B have a large effect on rachis fragility. The estimated locations of these QTLs corresponded to those of the Br genes identified in the previous studies on a more adapted durum wheat (T turgidum ssp. turgidum cony. durum). Our results indicate that selection and conversion of at least two Br loci (on chromosomes 2A and 3B) occurred during the domestication of emmer wheat prior to appearance of free-threshing wheat (e.g. durum). The map positions of nine QTLs for the traits related to seed production overlapped in two regions on chromosomes 2A and 5B. The result suggests that these chromosomal regions played an important role in increasing seed production during the domestication of emmer wheat. Biotechnol. & Biotechnol. Eq. 2013, 27(2), 3627-3637

    DIAGNOSIS PRESS LTD, 2013年04月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 27 (2), 3627 - 3637, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mai Tsujimura, Naoki Mori, Hiroshi Yamagishi, Toru Terachi

    In wheat (Triticum) and Aegilops, chloroplast and mitochondrial genomes have been studied for over three decades to clarify the phylogenetic relationships among species, and most of the maternal lineages of polyploid species have been clarified. Mitochondrial genomes of Emmer (tetraploid with nuclear genome AABB) and Dinkel (hexaploid with AABBDD) wheat are classified into two different types, VIIa and VIIb, by the presence-absence of the third largest HindIII fragment (named H3) in the mitochondrial DNA. Although the mitochondrial genome in the genera often provides useful information to clarify the phylogenetic relationship among closely related species, the phylogenetic significance of this dimorphism has yet not been clarified. In this study, to facilitate analysis using a large number of accessions, a sequence characterized amplified region (SCAR) marker that distinguishes the type VIIb mitochondrial genome from type VIIa was first developed. Mitochondrial genome type was determined for each of 30 accessions of wild and cultivated Emmer wheat and 25 accessions of Dinkel wheat. The mitochondrial genome type for each accession was compared with the plastogroup that had been determined using chloroplast microsatellite markers. Unexpectedly, the distribution of mitochondrial genome type was not in accordance with that of the plastogroups, suggesting occasional paternal leakage of either the mitochondrial or chloroplast genome during speciation and differentiation of Emmer and Dinkel wheat. An alternative possibility that substoichiometric shifting is involved in the observed dimorphism of the mitochondrial genome is also discussed.

    CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS, 2013年04月, GENOME, 56 (4), 187 - 193, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hien Thi Thu Vu, Oliver Escano Manangkil, Naoki Mori, Shinya Yoshida, Chiharu Nakamura

    Elongation escape mediated by seedling vigor with fast shoot elongation under submergence is considered to be an important strategy for rice particularly in direct seeding cultivation systems. We compared the expression profiles of two key genes in rice for ethylene biosynthesis - 1-aininocyclopropane-carboxylate synthase (OsACS) and oxidase (OsACO) genes, and five Na+/H+ exchanger (OsNHA) paralogs under normal and submerged conditions. The amount of transcripts was studied by RT-PCR using seedlings of one japonica and three indica cultivars with different levels of seedling vigor under submergence. In both types, submergence induced marked accumulation of transcripts of all paralogs of OsACS and OsACO except for OsACS1, which showed complete repression under the stress in non-vigorous indica cultivars suggesting its key role in seedling vigor under submergence. OsNHX expression was also enhanced under submergence, and the levels of OsNHX1 and OsNHX5 transcripts agreed well with those of seedling vigor under submergence. The observed mode of OsACS, OsACO and OsNHX expression under submergence suggests that these genes can be potential targets for understanding the mechanism regulating seedling vigor under submergence at the post-germination stage in rice.

    DIAGNOSIS PRESS LTD, 2012年06月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 26 (3), 2945 - 2951, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Aloka Lanka Ranawake, Naoki Mori, Chiharu Nakamura

    Abiotic stress is one of the major limiting factors reducing crop yield worldwide. We studied the expression profiles of 12 known stress responsive genes including transcription factor genes in japonica standard rice cultivar Nipponbare under low temperature, drought, NaCl and ABA stresses by RT-PCR in a time series up to 24 h. Expression of all the selected candidate genes was induced at least under one abiotic stress. The observed levels of expression of the same genes differed under different stress conditions; some were positively regulated under some stresses but negatively regulated under other stresses. Our results showed the presence of both specific and common gene regulatory pathways for these stresses and provide information on selective markers useful in evaluating cereal germplasms.

    DIAGNOSIS PRESS LTD, 2012年04月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 26 (2), 2838 - 2843, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 葉緑体DNAの変異からみたインド亜大陸のエンマーコムギとインド矮性コムギの遺伝的多様性と起源について

    森 直樹, 高木 俊弥, 千葉 一, 大田 正次

    インド亜大陸で数千年にわたって栽培されてきたエンマーコムギとインド矮性コムギの起源と伝播を明らかにするため、母性遺伝する葉緑体DNAのマイクロサテライト多型を詳細に解析した。その結果、現在インドで伝統的に栽培されているエンマーコムギは予想に反してアラビア海の交易とともに海のルートで北アフリカから伝播した可能性が高いことが明らかになった。また、フィールドでの現地調査から、全世紀半ばにその栽培記録が途絶えていたインド矮性コムギが現在でも栽培されていることが再発見された。

    2012年04月, 環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2010-11年度成果報告書, 73 - 84, 日本語

    研究論文(大学,研究機関等紀要)

  • インドにおけるエンマーコムギの栽培と利用—現状とその意味すること

    大田 正次, 森 直樹, 千葉 一

    インダス文明期から栽培さされてきたエンマーコムギの栽培と利用を現地における調査から明らかにするため、インド南部のニルギリ高原からカルナータカ州を経てマハーラーシュトラ州にかけて踏査した。この結果をもとにエンマーコムギの伝統的栽培とその利用について報告する。

    総合地球環境学研究所, 2012年04月, 「環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2010-11年度成果報告書, 65 - 72, 日本語

    研究論文(大学,研究機関等紀要)

  • Differential expression Nilaparvata lugens (Stål) (Homoptera: Delphacidae) with different levels of virulence.

    Nirosha Samanmali Gamalath, Muhammad Tufail, Prem Nidhi Sharma, Naoki Mori, Makio Takeda, Chiharu Nakamura

    2012年04月, Entomol. Zool., 47, 9 - 16, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 葉緑体DNAの変異からみたインド亜大陸のエンマーコムギとインド矮性コムギの遺伝的多様性と起源について

    森 直樹, 高木 俊弥, 千葉 一, 大田 正次

    総合球環境学研究所, 2012年02月, 「環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2010-11年度成果報告書, H03, 73 - 84, 日本語

    研究論文(大学,研究機関等紀要)

  • インドにおけるエンマーコムギの栽培と利用—現状とその意味すること

    大田 正次, 森 直樹, 千葉一

    総合地球環境学研究所, 2012年02月, 総合地球環境学研究所プロジェクトH-03 「環境変化とインダス文明」2010-11年度成果報告書, H03, 65 - 72, 日本語

    研究論文(大学,研究機関等紀要)

  • Nirosha Samanmali Gamalath, Muhammad Tufail, Prem Nidhi Sharma, Naoki Mori, Makio Takeda, Chiharu Nakamura

    Controlling the brown planthopper (BPH), Nilaparvata lugens (Stal) (Hemiptera: Delphacidae), is a difficult task in rice (Oryza sativa L.) production. We focused on vitellogenins (Vg), which are the major yolk protein precursors of vitellins and play an important role in the reproduction of oviparous species, including insects. We studied the accumulation of Vg mRNA and protein in a virulent BPH strain, Nagasaki-03, and a nonvirulent strain, Hatano-66, after rearing them on four rice lines. The rice lines used were two single resistance gene introgression lines, Norin-PL3 (Bph1 carrier) and Norin-PL4 (bph2 carrier), a pyramided line in which both genes were combined, and a susceptible japonica recurrent parent Tsukushibare. RT-PCR and quantitative RT-PCR analyses showed that the Vg mRNA level decreased greatly in Hatano-66 on the resistant lines. In contrast, the level of reduction on the resistant lines was much less in Nagasaki-03. Immunoblot analysis showed that Nagasaki-03 retained comparable levels of 175 kDa Vg protein on both the susceptible and resistant lines, whereas in Hatano-66, no Vg protein was detected on the resistant lines. Our results showed that BPH resistance genes caused differential reduction in the accumulation of Vg mRNA and protein, leading to the retardation of BPH reproduction on the resistant host rice plants.

    SPRINGER TOKYO, 2012年02月, APPLIED ENTOMOLOGY AND ZOOLOGY, 47 (1), 9 - 16, 英語

    [査読有り]

    研究論文(学術雑誌)

  • QTL analysis for submergence avoidance using backcross recombinant inbred lines between Oryza sativa and O. rufipogon.

    Phuong Dang, Thai Phan, Pham Thien Thanh, Naoki Mori, Ryo Ishikawa, Takashige Ishii

    2011年, Journal of Crop research, 56, 45 - 49, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Asakura, Naoki Mori, Chiharu Nakamura, Ichiro Ohtsuka

    Common wheat (Triticum aestivum) evolved through hybridization between cultivated tetraploid emmer wheat (T. turgidum), which has A and B genomes, and the wild diploid species, Aegilops tauschii, which has the D genome. Although the evolution of common wheat is generally understood, specific details remain unclear. For example, the phylogenetic relationships and origins of the six wheat subspecies (ssp. spelta, macha, vavilovi, aestivum, compactum, and sphaerococcum) have not yet been thoroughly resolved. To clarify the origin of ssp. sphaerococcum, we employed comparative sequence analysis of the D genome-specific sequence-tagged-site (STS) locus A1 in common wheat accessions, including sphaerococcum. Only the two known alleles, type A and type B were found among the accessions. Of the two sphaerococcum accessions, both possessed the type A allele. Four aestivum accessions also possessed the type A allele, while the remaining three accessions possessed the type B allele. Conversely, the accessions of the four remaining subspecies possessed the type B allele. Since sphaerococcum has morphological traits that differ from aestivum and which are pleiotropically regulated by a single recessive gene designated s, sphaerococcum most likely originated from aestivum, with the type A allele at the A1 locus arising through a spontaneous mutation at the s locus.

    2011年, Breeding Science, 61 (2), 212 - 216, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Pham Thien Thanh, Phuong Dang Thai Phan, Naoki Mori, Ryo Ishikawa, Takashige Ishii

    Backcross populations at advanced generations are useful in evaluating the agronomic traits from unadapted germplasms, such as wild species. In this study, we generated 159 backcross recombinant inbred lines (BRILs) at the BC2F8 generation between Oryza sativa Nipponbare (a recurrent parent) and O. rufipogon W630 (a donor parent). These BRILs were found to have nearly homozygous genome constitutions based on the marker genotypes at 180 SSR loci covering whole chromosomes. Since their selfed progenies have almost identical genotypes in each line, they were further used for QTL analysis of drought tolerance. Out of six QTLs detected, three were found to have wild alleles with favorable effects on drought tolerance. The present BRILs with known marker data can be applied to more QTL analyses of traits specific to wild species.

    Jpn Soc. Plant Breed., 2011年, Breeding Science, 61 (1), 76 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Post-germination seedling vigor under submergence and submergence-induced SUB1A gene expression in indica and japonica rice (Oryza sativa L.)

    H. T. T. Vu, O. E. Manangkil, N. Mori, S. Yoshida, C. Nakamura

    We evaluated post-germination seedling vigor in indica and japonica rice under submergence based on shoot elongation and recovery after submergence. The japonica cultivars showed more vigorous shoot elongation than indica cultivars under submergence in the dark, whereas an opposite response was observed under the light-dark submergence. Both Nipponbare and FR13A that are respectively known as submergence intolerant and tolerant cultivars showed vigorous shoot growth under submergence and high recovery after the stress, irrespective of the light/dark conditions. The results show that vigorous shoot growth enables rice seedlings to escape and survive submergence stress. RT-PCR analysis showed that expression of SUB1A, which is known as a key gene controlling submergence tolerance, was induced in elongating seedlings of FR13A and other indica cultivars including floating rice under submergence with light illumination. Our results suggest that light-induced SUB1A gene expression does not hinder the shoot elongation response under submergence in rice at the post-germination seedling stage.

    SOUTHERN CROSS PUBL, 2010年06月, AUSTRALIAN JOURNAL OF CROP SCIENCE, 4 (4), 264 - 272, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 生業システム研究グループ2009年度活動報告

    大田 正次, 千葉 一, 森 直樹, 三浦 励一

    環境変化とインダス文明の関係を遺跡から出土する植物遺物をもとにした古民族植物学的研究と現存する栽培植物の利用をもとにした民族植物学的研究の両面から探ることを目的に、インド中南部においてフィールド調査を行った。

    2010年04月, 「環境変化とインダス文明」総合地球環境学研究所プロジェクトH-03 2009年度成果報告書, 93 - 102, 日本語

    研究論文(大学,研究機関等紀要)

  • Post-germination seedling vigor under submergence and submergence-induced SUB1A gene expression in indica and japonica rice (Oryza sativa L.)

    H. T. T. Vu, O. E. Manangkil, Naoki Mori, S. Yoshida, C. Nakamura

    We evaluated post-germination seedling vigor in indica and japonica rice under submergence based on shoot elongation and recovery after submergence. The japonica cultivars showed more vigorous shoot elongation than indica cultivars under submergence in the dark, whereas an opposite response was observed under the light-dark submergence. Both Nipponbare and FR13A that are respec

    2010年, Australian J. Crop Science, 4 (4), 264 - 272, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shotaro Takenaka, Naoki Mori, Taihachi Kawahara

    Because of the huge morphological variations exhibited there, the Abyssinian Highlands (Ethiopia) are thought of as the secondary center of diversity of domesticated emmer wheat (Triticum turgidum L.). The genetic diversity in 88 domesticated emmer wheat accessions from Ethiopia and other regions was analyzed using DNA sequences of parts of four genes, Acc1-A, Pgk1-B, Waxy-A and Waxy-B, which are single-copy genes in nuclear DNA. The multilocus genotypes determined by analysis using Structure enabled the 88 accessions to be divided into four clusters. Each cluster included both hulled and free-threshing subspecies, and there was no clustering corresponding to specific subspecies. Both hulled and free-threshing emmer landraces in Ethiopia had several characteristic alleles, and the gene flow between them was shown to be limited. The genetic composition of Ethiopian landraces differed from that of emmer in other regions of the world. However, their nucleotide diversity was not as high as that of other emmer populations. The large morphological diversity in Ethiopian emmer landraces must have evolved from a limited genetic background.

    2010年, Breeding Science, 60 (3), 212 - 227, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hakan Ozkan, Metin Tuna, Benjamin Kilian, Naoki Mori, Shoji Ohta

    Background and aims Intra-and interspecific variations of C-values and the relationship between habitat factors and genome size were studied in natural populations of diploid and tetraploid wild wheats. Methodology The 1C nuclear DNA content of 376 individual plants representing 41 populations of diploid and tetraploid wild wheats was determined by flow cytometry (FCM) and correlated with geographical and bioclimate variables. Principal results Based on analysis of variance, significant differences between diploid and tetraploid Triticum species were found. Differences among populations of T. boeoticum and T. dicoccoides were also statistically significant and argue for isolation between populations, except for T. araraticum. However, the variation among individuals of the same population was not statistically significant. Maximum genome size differences among populations for T. boeoticum (0.143 pg; 2.32%), T. dicoccoides (0.314 pg; 2.49%) and T. araraticum (0.116 pg; 0.98 %) argue for genome constancy in these species. There was no significant correlation between intra-population variance and geographical and bioclimate variables for T. boeoticum and T. dicoccoides. In contrast to the limited genome size variation at the intraspecific level, the interspecific variation was large: similar to 0.5 pg/1C (8 %) at the diploid level (T. boeoticum vs. T. urartu) and similar to 1 pg/1C (9.7 %) at the tetraploid level (T. dicoccoides vs. T. araraticum). Conclusions Low intraspecific genome size variation occurs in diploid and tetraploid wild wheats, and this limited variation is not correlated with geographical and climate variables. However, interspecific variation is significant at the diploid and tetraploid level. It can be concluded that the genome size of wild self-fertilizing Triticum species is generally stable, despite the presence of many potentially active retroelements. In natural habitats, it is very difficult to distinguish wild wheats from each other. However, all four species can be distinguished easily, quickly and unambiguously by using the FCM technique.

    OXFORD UNIV PRESS, 2010年, AOB PLANTS, 1 - 11 (plq015), 英語

    [査読有り]

    研究論文(学術雑誌)

  • Genetic diversity and origin of timopheevi wheat inferred by chloroplast DNA fingerprinting

    Naoki Mori, Yasutaka Kondo, Takashige Ishii, Taihachi Kawahara, Jan Valkoun, Chiharu Nakamura

    Domesticated timopheevi wheat (Triticum timopheevi) is an endemic crop of western Georgia in Transcaucasia. It has a distinct nuclear genome (2n=28, AAGG) and is genetically isolated from other wheat species. To clarify the genetic diversity and the domestication of this interesting wheat, we analyzed molecular variation at 23 microsatellite loci in the chloroplast genome. Allelic diversity was evaluated using 94 accessions representing domesticated timopheevi wheat (T. timopheevi), wild timopheevi wheat (T. araraticum), and wild emmer wheat (T. dicoccoides). The average diversity index (H) in T araraticum (0.206) was smaller than that in T. dicoccoides (0.284). No polymorphisms were detected among the six accessions of T. timopheevi, suggested a monophyletic origin of domesticated timopheevi wheat. Phylogenetic analyses of the plastotypes revealed clear differences between the chloroplast DNA of timopheevi wheat and emmer wheat, and thus supported the hypothesis that these two wheat species originated independently. None of the T. araraticum plastotypes collected in Transcaucasia were closely related to the T. timopheevi plastotype. On the other hand, the plastotypes found in northern Syria and southern Turkey showed closer relationships with T. timopheevi. These results suggested that the domestication of timopheevi wheat might have occurred in the region including southern Turkey and northern Syria.

    JAPANESE SOC BREEDING, 2009年12月, BREEDING SCIENCE, 59 (5), 571 - 578, 英語

    [査読有り]

    研究論文(学術雑誌)

  • H. T. T. Vu, O. E. Manangkil, N. Mori, S. Yoshida, C. Nakamura

    ADH and ALDH gene expression was studied by reverse transcriptase polymerase chain reaction (RT-PCR) using selected indica and japonica rice cultivars showing vigorous and non-vigorous post-germination seedling growth under submergence stress. Transcript amount of ADH1, ADH2 and ALDH2a increased dramatically under submergence irrespective of dark/light conditions in both indica and japonica rice, whereas ALDH2b gene expression was greatly suppressed. Submergence-induced increases of the transcript levels also occurred in floating rice as well as in selected chromosome segment substitution lines that were derived from crosses between 'Nipponbare' and 'Kasalath'. However, no cultivar and line differences were observed in their transcript levels under submergence. Our results suggest that the expression of ADH1, ADH2 and ALDH2a genes is highly responsive to submergence stress but is not a major determinant of the cultivar/genotype-specific level of seedling vigor under submergence.

    DIAGNOSIS PRESS LTD, 2009年11月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 23 (4), 1469 - 1473, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Differential cDNA-AFLP screening of transcripts associated with brown planthopper resistance in rice (Oryza sativa L.)

    N. S. Gamalath, P. N. Sharma, N. Mori, C. Nakamura

    We have previously constructed a pyramid line, in which two major BPH resistance genes, Bph1 and Bph2 on the long arm of rice chromosome 12, were introgressed into a susceptible japonica cultivar, Tsukushibare. To identify transcripts that are derived from the introgressed chromosomal segments carrying the resistance genes, we analyzed mRNA profiles using amplified fragment length polymorphism-based mRNA fingerprinting (cDNA-AFLP). After this differential screening using 423 primer combinations, 41 polymorphic fragments were selected that were specific either to the pyramid line or Tsukushibare. Among them, 10 fragments expressed only in the pyramid line were cloned and sequenced. According to the genome database, at least two were from the proximity of the introgressed Bph1 locus. Our results suggest that cDNA-AFLP screening of differentially expressed fragments is a useful means to identify BPH resistance and/or infestation associated genes.

    SOUTH CROSS PUBL, 2009年07月, AUSTRALIAN JOURNAL OF CROP SCIENCE, 3 (4), 201 - 206, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Characterization of brown planthopper strains based on their differential responses to introgressed resistance genes and on mitochondrial DNA polymorphism

    Muhammad NAEEMULLAH, Prem N. SHARMA, Muhammad TUFAIL, Naoki Mori, Masaya MATSUMURA, Makio TAKEDA, Chiharu NAKAMURA

    Biotypes cause a serious problem in controlling brown planthopper (BPH), Nilaparvata lugens Stål (Homoptera: Delphacidae).
    We characterized four BPH strains, which were collected in different localities in Japan and maintained by
    random mating within each strain for different periods. The virulence of the BPH strains was evaluated based on their
    postembryonic development on sus

    2009年05月, Appl. Entomol. Zool., 44 (3), 475 - 483, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species

    Naoki Mori, Yuko Hidehira, Takashige Ishii, Chiharu Nakamura

    The domestication of wheat and barley was the most important step in the emergence of farming communities that led to the ancient civilizations of Mesopotamia. Several lines of evidence indicate that emmer wheat was the earliest form of domesticated wheat derived from wild emmer wheat. Chloroplast DNA fingerprinting of wild and domesticated emmer wheat revealed that two dis

    2009年, Proc. 4th International Conference on Energy Efficiency and Agricultural Engineering, 725 - 729, 英語

    研究論文(国際会議プロシーディングス)

  • Nobuaki Asakura, Naoki Mori, Chiharu Nakamura, Ichiro Ohtsuka

    The Q locus located on the long arm of chromosome 5A is a key factor in evolution and widespread cultivation of domesticated wheat. The Q locus pleiotropically affects many agronomically important traits including threshability, glume shape and tenacity, rachis fragility and others. Genotyping of the Q locus based on the complex traits is ambiguous due to their multi-genetic control through interactions with the Q locus. To determine the Q locus genotype of wheat accessions possessing A genome, we developed a method based on polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis. The Q and q alleles were clearly distinguished by PCR-RFLP analysis at six conserved single nucleotide polymorphisms in common wheat and wild and cultivated einkorn, emmer and timopheevi wheat. The Q locus genotype of Triticum sinskajae, which is one of the einkorn wheat species and exhibits free-threshing trait, was determined to be qq as expected. This simple PCR-RLFP-based genotyping method should serve as a useful tool in studying the origin of Q and thus wheat evolution after domestication and the following widespread cultivation.

    Jpn Soc. Genetics, 2009年, Genes and Genetic Systems, 84 (3), 233 - 237, 英語

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    研究論文(学術雑誌)

  • Oliver E. Manangkil, Hien Thi Thu Vu, Shinya Yoshida, Naoki Mori, Chiharu Nakamura

    Submergence is a major stress causing yield losses particularly in the direct-seeded rice cultivation system and necessitates the development of a simple, rapid and reliable bioassay for a large scale screening of rice germplasms with tolerance against submergence stress. We developed two new bioassay methods that were based primarily on the seedling vigor evaluated by the ability of fast shoot elongation under submerged conditions, and compared their effectiveness with two other available methods. All four bioassay methods using cultivars of 7 indica and 6 japonica types revealed significant and consistent cultivar differences in seedling vigor under submergence and/or submergence tolerance. Japonica cultivars were more vigorous than indica cultivars, with Nipponbare being the most vigorous. The simplest test tube method showed the highest correlations to all other methods. Our results suggest that seedling vigor serves as a submergence avoidance mechanism and confers tolerance on rice seedlings to flooding during early crop establishment. A possible relationship is discussed between seedling vigor based on fast shoot elongation and submergence tolerance defined by recovery from submergence stress.

    SPRINGER, 2008年09月, EUPHYTICA, 163 (2), 267 - 274, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mapping of quantitative trait loci associated with cold tolerance at the post-germination stage in rice

    A. L. Ranawake, T. Ishii, N. Mori, S. Yoshida, C. Nakamura

    Cold tolerance at the post-germination stage in rice was studied using gained shoot length (GSL) as a new parameter which was measured as increased shoot length during cold stress at 4 degrees C followed by a 4-day recovery period at 35 T. GSL decreased until 4(th) day in both japonica and indica cultivar while a marked increase occurred in a japonica cultivar after 5 days of cold stress, indicating its higher ability of cold acclimation. Quantitative trait loci (QTLs) associated with cold tolerance were identified using recombinant inbred lines derived from these parents on a linkage map constructed with 102 simple sequence repeat (SSR) markers. By the interval analysis, one and five putative QTLs were identified after 5 and 11 days of cold stress, respectively. The single point analysis indicated that these QTLs explained 16% and 54% of the phenotypic variance after 5 and 11 days of cold stress, respectively. A cold tolerant japonica parent contributed all QTLs, among which one on the long arm of chromosome 7 was common for the two cold stress periods. Based on gained root length one QTL was identified on chromosome 12 explaining 11% of the variance.

    DIAGNOSIS PRESS LTD, 2008年02月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 22 (1), 536 - 540, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Asakura, Shinya Yoshida, Naoki Mori, Ichiro Ohtsuka, Chiharu Nakamura

    Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkom wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I - and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The number of copies of subclass II elements was much higher in Aegilops with the S, Sl and D genomes and polyploid Triticum species than in diploid Triticum with the A genome, indicating that active transposition occurred in S, Sl and D genomes before polyploidization. DNA gel blot analysis of six species selected from three subfamilies of Poaceae demonstrated that only the tribe Triticeae possessed both subclasses. These results suggest that the differentiation of these two subclasses occurred before or immediately after the establishment of the tribe Triticeae. Copyright © 2008, Sociedade Brasileira de Genética.

    Brazilian Journal of Genetics, 2008年, Genetics and Molecular Biology, 31 (2), 539 - 546, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mitochondrial microsatellite variability in common wheat and its ancestral species

    Takashige Ishii, Chisa Takahashi, Nobuyuki Ikeda, Osamu Kamijima, Naoki Mori

    On the basis of the entire mitochondrial DNA sequence of common wheat, Triticum aestivum, 21 mitochondrial microsatellite loci having more than ten mononucleotide repeats were identified. The mitochondrial microsatellite variability at all loci was examined with 43 accessions from 11 Triticum and Aegilops species involved in wheat polyploidy evolution. Polymorphic banding patterns were obtained at 15 out of 21 mitochondrial microsatellite loci. The number of alleles per polymorphic microsatellite ranged from 2 to 5 with an average of 3.07, and the diversity values (H) ranged from 0.09 to 0.50 with an average of 0.29. These values are almost two third of wheat chloroplast microsatellite values, indicating that variability of mitochondrial microsatellite is much less than that of chloroplast microsatellite. Based on the allele variation at all loci, a total of seven mitochondrial haplotypes were identified among common wheat and its ancestral species. Three diploid species showed their own specific haplotypes and timopheevi group (11 accessions) had three types, whereas 29 accessions of emmer and common wheat groups shared the same haplotype. These results indicate that a single mitochondrial haplotype determined by microsatellite analysis has conservatively been maintained in the evolutionary lineage from wild tetraploid to cultivated hexaploid species.

    GENETICS SOC JAPAN, 2006年06月, GENES & GENETIC SYSTEMS, 81 (3), 211 - 214, 英語

    [査読有り]

    研究論文(学術雑誌)

  • HT Gandhi, MI Vales, CJW Watson, CA Mallory-Smith, N Mori, M Rehman, RS Zemetra, O Riera-Lizarazu

    Aegilops cylindrica Host (2n=4x=28, genome CCDD) is an allotetraploid formed by hybridization between the diploid species Ae. tauschii Coss. (2n=2x=14, genome DD) and Ae. markgrafii (Greuter) Hammer (2n=2x=14, genome CC). Previous research has shown that Ae. tauschii contributed its cytoplasm to Ae. cylindrica. However, our analysis with chloroplast microsatellite markers showed that 1 of the 36 Ae. cylindrica accessions studied, TK 116 (PI 486249), had a plastome derived from Ae. markgrafii rather than Ae. tauschii. Thus, Ae. markgrafii has also contributed its cytoplasm to Ae. cylindrica. Our analysis of chloroplast and nuclear microsatellite markers also suggests that D-type plastome and the D genome in Ae. cylindrica were closely related to, and were probably derived from, the tauschii gene pool of Ae. tauschii. A determination of the likely source of the C genome and the C-type plastome in Ae. cylindrica was not possible.

    SPRINGER, 2005年08月, THEORETICAL AND APPLIED GENETICS, 111 (3), 561 - 572, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y Matsuoka, N Mori, T Kawahara

    Intraspecific patterns of chloroplast DNA variation was studied in Aegilops tauschii Coss., the D-genome progenitor of bread wheat. Nucleotide sequences of ten chloroplast microsatellite loci were analyzed for 63 accessions that cover the central part of the species distribution. As is often the case with nuclear microsatellites, those of chloroplasts of Ae. tauschii bear complex mutations. Several types of mutations other than change in the microsatellite repeat number were found, including base substitutions and length mutations in flanking regions. In total, eight mutations were present in the flanking regions of four loci. Most mutations in the flanking regions of microsatellite repeats are associated with biallelic polymorphisms. Phylogeographic analyses showed that such biallelic polymorphisms are useful to investigate intraspecific patterns of monophyletic lineage divergence. In contrast, most microsatellite repeat sites are multiallelic, variable within intraspecific lineages, and useful to compare degrees of genetic diversity between lineages. These findings show that the chloroplast genome harbors evolutionary variations informative for intraspecific studies of Ae. tauschii and can be analyzed by genealogical approaches.

    SPRINGER, 2005年07月, THEORETICAL AND APPLIED GENETICS, 111 (2), 265 - 271, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y Ogihara, Y Yamazaki, K Murai, A Kanno, T Terachi, T Shiina, N Miyashita, S Nasuda, C Nakamura, N Mori, S Takumi, M Murata, S Futo, K Tsunewaki

    The application of a new gene-based strategy for sequencing the wheat mitochondrial genome shows its structure to be a 452 528 bp circular molecule, and provides nucleotide-level evidence of intra-molecular recombination. Single, reciprocal and double recombinant products, and the nucleotide sequences of the repeats that mediate their formation have been identified. The genome has 55 genes with exons, including 35 protein-coding, 3 rRNA and 17 tRNA genes. Nucleotide sequences of seven wheat genes have been determined here for the first time. Nine genes have an exon-intron structure. Gene amplification responsible for the production of multicopy mitochondrial genes, in general, is species-specific, suggesting the recent origin of these genes. About 16, 17, 15, 3.0 and 0.2% of wheat mitochondrial DNA (mtDNA) may be of genic (including introns), open reading frame, repetitive sequence, chloroplast and retro-element origin, respectively. The gene order of the wheat mitochondrial gene map shows little synteny to the rice and maize maps, indicative that thorough gene shuffling occurred during speciation. Almost all unique mtDNA sequences of wheat, as compared with rice and maize mtDNAs, are redundant DNA. Features of the gene-based strategy are discussed, and a mechanistic model of mitochondrial gene amplification is proposed.

    OXFORD UNIV PRESS, 2005年, NUCLEIC ACIDS RESEARCH, 33 (19), 6235 - 6250, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Z Hashimoto, N Mori, M Kawamura, T Ishii, S Yoshida, M Ikegami, S Takumi, C Nakamura

    Japanese rice (Oryza sativa L.) cultivars that are strictly used for the brewing of sake (Japanese rice wine) represent a unique and traditional group. These cultivars are characterized by common traits such as large grain size with low protein content and a large, central white-core structure. To understand the genetic diversity and phylogenetic characteristics of sake-brewing rice, we performed amplified fragment length polymorphism and simple sequence repeat analyses, using 95 cultivars of local and modern sake-brewing rice together with 76 cultivars of local and modern cooking rice. Our analysis of both nuclear and chloroplast genome polymorphisms showed that the genetic diversity in sake-brewing rice cultivars was much smaller than the diversity found in cooking rice cultivars. Interestingly, the genetic diversity within the modern sake-brewing cultivars was about twofold higher than the diversity within the local sake-brewing cultivars, which was in contrast to the cooking cultivars. This is most likely due to introgression of the modern cooking cultivars into the modern sake-brewing cultivars through breeding practices. Cluster analysis and chloroplast haplotype analysis suggested that the local sake-brewing cultivars originated monophyletically in the western regions of Japan. Analysis of variance tests showed that several markers were significantly associated with sake-brewing traits, particularly with the large white-core structure.

    SPRINGER, 2004年11月, THEORETICAL AND APPLIED GENETICS, 109 (8), 1586 - 1596, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chloroplast and nuclear DNA variation in common wheat: insight into the origin and evolution of common wheat

    S Hirosawa, S Takumi, T Ishii, T Kawahara, C Nakamura, N Mori

    To understand the origin and evolution of common wheat, chloroplast (ct) and nuclear DNA variations were studied in five hexaploid and three tetraploid wheat subspecies. Based on chloroplast simple sequence repeats at 24 loci, they were classified into two major plastogroups. Plastogroup I consisted of 11 plastotypes, including the major plastotype H10 that occurred at the highest frequency (59%) in common wheat. Plastogroup 11 consisted of five plastotypes and occurred in eight out of 27 accessions of T aestivum ssp. spelta and one accession of ssp. aestivum. As for nuclear DNA variations, AFLP data using 10 primer sets revealed two major clades of a phylogenetic tree constructed by UPGMA (unweighted pair-group method with arithmetic mean), one consisting of common wheat and the other of emmer wheat. The clade of common wheat was further divided into two major and six minor subclades. One of the major subclades consisted only of non-free-threshing ssp. spelta accessions, which were grouped into two clusters, one consisting only of accessions with plastogroup I ctDNA and the other with both plastogroups I and IT. T. aestivum ssp. macha, another non-free-threshing common wheat, formed the other cluster. Taken together, our data indicate the existence of at least two maternal lineages in common wheat and support the hypothesis that European spela wheat originated in Europe separately from other groups of common wheat.

    GENETICS SOC JAPAN, 2004年10月, GENES & GENETIC SYSTEMS, 79 (5), 271 - 282, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Z. Hashimoto, N. Mori, M. Kawamura, T. Ishii, S. Yoshida, W. Ikegami, S. Takumi, C. Nakamura

    2004年09月, Theoretical and Applied Genetics, 109, 1586 - 1596

    [査読有り]

    研究論文(学術雑誌)

  • Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus

    Y Tosa, K Hirata, H Tamba, S Nakagawa, Chuma, I, C Isobe, J Osue, AS Urashima, LD Don, M Kusaba, H Nakayashiki, A Tanaka, T Tani, N Mori, S Mayama

    Fungal isolates from gray leaf spot on perennial ryegrass (prg isolates) were characterized by DNA analyses, mating tests, and pathogenicity assays. All of the prg isolates were interfertile with Triticum isolates and clustered into the c top isolate group (CC group) on a dendrogram constructed from rDNA-internal transcribed spacer 2 sequences. Since the CC g-roup corresponded to a newly proposed species, Magnaporthe oryzae, all of the prg isolates were designated M. oryzae. However. DNA finger-printing with MGR586, MGR583. and Pot2 showed that the prg isolates are divided into two distinct populations, i.e., TALF isolates and WK isolates. The TALF isolates were virulent only on Lolium species, whereas the WK isolates were less specific. suggesting that Way leaf spot can he caused not only by Lolium-specific isolates but also by less specific isolates. We designated the TALF isolates as Lolium pathotype. The TALF isolates showed diverse karyotypes in spite of being uniform in DNA fingerprints, Suggesting that they are unstable in genome organization.

    AMER PHYTOPATHOLOGICAL SOC, 2004年05月, PHYTOPATHOLOGY, 94 (5), 454 - 462, 英語

    研究論文(学術雑誌)

  • Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stal) resistance genes Bph1 and Bph2 on rice chromosome 12

    PN Sharma, A Torii, S Takumi, N Mori, C Nakamura

    Brown planthopper (BPH) (Nilaparvata lugens Stal) is a significant insect pest of rice (Oryza sativa L.). We constructed a gene-pyramided japonica line, in which two BPH resistance genes Bph1 and Bph2 on the long arm of chromosome 12 independently derived from two indica resistance lines were combined through the recombinant selection. The gene-pyramiding was achieved based on the previously constructed high-resolution linkage maps of the two genes. Two codominant and four dominant PCR-based markers flanking the loci were used to select for a homozygous recombinant line in a segregating population that was derived from a cross between the parental homozygous single-gene introgression lines. BPH bioassay showed that the resistance level of the pyramided line was equivalent to that of the Bph1-single introgression line, which showed a higher level of resistance than the Bph2-single introgression line. The pyramid line should provide a useful experimental means for studying the fine structure of the chromosomal region covering these two major BPH resistance genes.

    BLACKWELL MUNKSGAARD, 2004年02月, HEREDITAS, 140 (1), 61 - 69, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Y. Tosa, K. Hirata, H. Tamba, S. Nakagawa, I. Chuma, C. Isobe, J. Osue, A. S. Urashima, L. D. Don, M. Kusaba, H. Nakayashiki, A. Tanaka, T. Tani, N. Mori, S. Mayama

    Fungal isolates from gray leaf spot on perennial ryegrass (prg isolates) were characterized by DNA analyses, mating tests, and pathogenicity assays. All of the prg isolates were interfertile with Triticum isolates and clustered into the crop isolate group (CC group) on a dendrogram constructed from rDNA-internal transcribed spacer 2 sequences. Since the CC group corresponded to a newly proposed species, Magnaporthe oryzae, all of the prg isolates were designated M. oryzae. However, DNA fingerprinting with MGR586, MGR583, and Pot2 showed that the prg isolates are divided into two distinct populations, i.e., TALF isolates and WK isolates. The TALF isolates were virulent only on Lolium species, whereas the WK isolates were less specific, suggesting that gray leaf spot can be caused not only by Lolium-specific isolates but also by less specific isolates. We designated the TALF isolates as Lolium pathotype. The TALF isolates showed diverse karyotypes in spite of being uniform in DNA fingerprints, suggesting that they are unstable in genome organization.

    American Phytopathological Society, 2004年, Phytopathology, 94 (5), 454 - 462, 英語

    研究論文(学術雑誌)

  • Marker-assisted pyramiding of two brown planthopper (Nolaparvata lugens Stal) resistance genes Bph1 and Bph2 in rice (Oryza genes Bph1 and Bph2 in rice (Oryza sativa L.)

    SHARMA P N, TORII A, TAKUMI Shigeo, MORI Naoki, NAKAMURA Chiharu

    2004年, Hereditas, 140: 61-69, 英語

    [査読有り]

    研究論文(学術雑誌)

  • RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stal) resistance gene Bph1 in rice

    N Sharma, Y Ketipearachchi, K Murata, A Torii, S Takumi, N Mori, C Nakamura

    We have constructed a linkage map of the rice brown planthopper ( BPH) resistance gene, Bph1. RFLP and AFLP markers were selected by the bulked segregant analysis and used in the mapping study of 262 F(2)s that were derived from a cross of ' Tsukushibare', a susceptible japonica cultivar, and ' Norin- PL3', an authentic japonica Bph1-introgression line. Twenty markers were mapped within a 28.9- cM region containing the Bph1 locus on the long arm of rice chromosome 12. Combining the result of segregation analysis of BPH resistance by the mass seedling test and that of the markers, the Bph1 locus was mapped within a 5.8- cM region between two flanking markers. The closest AFLP markers, em5814N and em2802N, was at 2.7 cM proximal to the Bph1 locus. Together with the previously constructed high- resolution map of bph2 locating the locus at ca. 10 cM proximal to the Bph1 locus, this improved version of the linkage map would facilitate pyramiding these two important BPH resistance genes.

    KLUWER ACADEMIC PUBL, 2003年, EUPHYTICA, 129 (1), 109 - 117, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Transferability of hexaploid wheat (Triticum aestivum) microsatellite markers to tetraploid species (T-turgidum) carrying A and B genomes

    NAB Jelodar, N Mori, C Nakamura

    Emmer is the tetraploid wheat (genome constitution: AABB) from which hexaploid or common wheat (Triticum aestivum L., genome:AABBDD) was derived. We examined transferability of 180 microsatellite markers from hexaploid wheat to wild emmer wheat [T. turgidum ssp. dicoccoides (Korn. Ex Asch. & Graebner) Thell.] and cultivated emmer wheat [T turgidum ssp. dicoccum (Schrank ex Shobler) Thell.]. Among the 180 primer pairs, 121 (67.2%) amplified microsatellite loci from the ssp. dicoccoides genome and 122 (67.8%) from the ssp. dicoccum genome. Between these two subspecies, polymorphic banding patterns were observed at 97 (53.5%) microsatellite loci. Five primer pairs.(2.8%) produced amplification products only from the ssp. dicoccoides genome while 7 other primer pairs (3.9%) produced products only from the ssp. dicoccum genome. Fifty microsatellite markers (27.8%) failed to amplify any products in these two subspecies of T. turgidum. The microsatellite markers we identified to show cross-species transferability should be useful in studies of gene mapping and genome evolution in tetraploid wheat.

    CEREAL RES INST, 2003年, CEREAL RESEARCH COMMUNICATIONS, 31 (3-4), 273 - 280, 英語

    研究論文(学術雑誌)

  • Towards molecular cloning of resistance genes against brown planthopper (Nilaparvata lugens Stal) in rice: a case study of natural insect resistance genes.

    SHARMA P N, NURATA K, TORII A, TAKUMI Shigeo, MORI Naoki, NAKAMURA Chiharu

    2003年, Trends in Entomol., 未記入, 英語

    研究論文(学術雑誌)

  • Tauschii Core Collection; an effective tool for utilize wheat D genome genetic resources.

    KAWAHARA T, TAKUMI Shigeo, MATSUOKA Y, MORI Naoki, YASUI Y

    2003年, Xth Int. WheatGenet. Symp., Paestum, Vol.2:584-586, 英語

    研究論文(学術雑誌)

  • Origins of domesticated emmer and common wheat inferred from chloroplast DNA fingerprinting.

    MORI Naoki, ISHII Takashige, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA Y

    2003年, Proc. Xth Int. Wheat Genet. Symp., Vol.1,pp.25-28, 英語

    研究論文(学術雑誌)

  • Origin of domesticated emmer and common wheat inferred from chloroplast DNA finger printing.

    MORI Naoki, ISHII T, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA S, NAKAMURA Chiharu

    2003年, Xth Int. Wheat Genet. Symp.Paestum, Vol.1:25-28, 英語

    研究論文(学術雑誌)

  • Mitochondrial genome and genes of common wheat, Triticum aestivum cv. Chinese Spring.

    OGIHARA Y, FUTO S, KANNO A, MIYASHITA N, NASUDA S, SHIINA T, TERAUCHI T, GUO C-H, NAKAMURA Chiharu, MORI Naoki, TAKUMI Shigeo, MURATA M, YAMAZAKI Y, MURAI K, MATSUOKA Y, TSUNEWAKI K

    2003年, Xth Int. Wheat Genet. Symp.Paestum, Vol.3:1020-1022, 英語

    研究論文(学術雑誌)

  • Y Ogihara, K Isono, T Kojima, A Endo, M Hanaoka, T Shiina, T Terachi, S Utsugi, M Murata, N Mori, S Takumi, K Ikeo, T Gojobori, R Murai, K Murai, Y Matsuoka, Y Ohnishi, H Tajiri, K Tsunewaki

    Structural features of the wheat plastome were clarified by comparison of the complete sequence of wheat chloroplast DNA with those of rice and maize chloroplast genomes. The wheat plastome consists or a 134,545-bp circular molecule with 20,703-bp inverted repeats and the same gene content as the rice and maize plastomes. However, some structural divergence was found even in the coding regions of genes. These alterations are due to illegitimate recombination between two short direct repeats and/or replication slippage. Overall comparison of chloroplast DNAs among the three cereals indicated the presence of some hot-spot regions for length mutations. Whereas the region with clustered tRNA genes and that downstream of rbcL showed divergence in a species-specific manner, the deletion patterns of ORFs in the inverted-repeat regions and the borders between the inverted repeats and the small single-copy region support the notion that wheat and rice are related more closely to cacti other than to maize.

    SPRINGER-VERLAG, 2002年01月, MOLECULAR GENETICS AND GENOMICS, 266 (5), 740 - 746, 英語

    研究論文(学術雑誌)

  • T. Ishii, N. Mori, Y. Ogihara

    Twenty four chloroplast microsatellite loci having more than ten mononucleotide repeats were identified from the entire chloroplast DNA sequence of common wheat, Triticum aestivum cv Chinese Spring. For each microsatellite, a pair of primers were designed to produce specific PCR products in the range of 100-200 bp. The allelic diversity at the microsatellite loci was evaluated using 43 accessions from 11 Triticum and Aegilops species involved in wheat polyploid evolution. Polymorphic banding patterns were obtained at 21 out of 24 chloroplast microsatellite loci. The three monomorphic microsatellites were found to be located in coding regions. For the polymorphic microsatellites, the number of alleles per microsatellite ranged from 2 to 7 with an average of 4.33, and the diversity values (H) ranged from 0.05 to 0.72 with an average of 0.47. Significant correlations (P< 0.01) were observed between the number of repeats and the number of alleles, and between the number of repeats and diversity value, respectively. The genetic diversity explained by chloroplast microsatellites and nuclear RFLP markers were compared using 22 tetraploid accessions. Although the number of alleles for nuclear RFLP markers was found to be higher than that for chloroplast microsatellites, similar diversity values were observed for both types of markers. Among common wheat and its ancestral species, the percentages of common chloroplast microsatellite alleles were calculated to examine their phylogenetic relationships. As a result, Timopheevi wheat species were clearly distinguished from other species, and Emmer and common wheat species were divided into two main groups, each consisting of a series of wild and cultivated species from tetraploid to hexaploid. This indicates that the two types of chloroplast genomes of common wheat might have independently originated from the corresponding types of wild and cultivated Emmer wheat species.

    2001年, Theoretical and Applied Genetics, 103 (6-7), 896 - 904, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nobuaki Asakura, Naoki Mori, Takao Ishido, Ichiro Ohtsuka, Chiharu Nakamura

    Homoeoalleles of Ncc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon of Aegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmp1A locus, which is located on chromosome 1A of T. timopheevi, was sequenced and converted to a PCR-based sequence-tagged-site (STS) marker. Five single nucleotide polymorphisms (SNPs) between T. timopheevi and T. turgidum. were detected in a 509-bp genomic DNA fragment. Based on the SNPs, the STS alleles in 164 accessions from emmer wheat, timopheevi wheat and two einkorn wheats, T. urartu and T. boeoticum were surveyed by PCR-RFLP analysis. The sequence comparisons and PCR-RFLP analyses revealed nine alleles based on six SNPs. These SNPs were highly conserved within each group of wheat, and all groups could be distinguished by particular combinations of the SNPs. All accessions of T. urartu had one unique STS allele as compared with the others. Our results indicate that the SNPs in the STS marker linked to the Ncc-tmp1A locus would be informative for studies of the differentiation of chromosome 1A in wheat.

    2001年, Genes and Genetic Systems, 76 (5), 295 - 304, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mitsuhiro Kawaguchi, Kazumasa Murata, Takashige Ishii, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

    A Sri Lankan indica rice (Oryza sativa L.) cultivar Babawee harboring a brown planthopper (BPH) (Nilaparvata lugens Stål) resistance gene bph4 was crossed as a male parent with two susceptible cultivars, either indica IR24 or japonica Tsukushibare. Segregation of the BPH resistance in the two crosses was studied by directly assaying the F2 phenotypes and by determining the F2 genotypes based on the F3 phenotypes. In both cross combinations, the segregation of the BPH resistance significantly deviated from the ratio expected for the single recessive gene model. Using bulked DNAs of homozygous resistant and susceptible F2s and the parents, seven out of 214 RFLP markers were selected as linked markers, of which six on chromosome 6 were common in the two cross combinations. Two micro-satellite markers on chromosome 6 were also linked to bph4. Although the map position of bph4 could not be determined, the gene was assigned to the distal region of the short arm of rice chromosome 6 based on the bulked segregant analysis and linkage analysis.

    2001年, Breeding Science, 51 (1), 13 - 18

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    研究論文(学術雑誌)

  • H. Murai, Z. Hashimoto, P. N. Sharma, T. Shimizu, K. Murata, S. Takumi, N. Mori, S. Kawasaki, C. Nakamura

    The brown planthopper (BPH), Nilaparvata lugens Stål, is a significant insect pest of rice (Oryza sariva L.). bph2 is one of the 12 major BPH resistance genes so far identified in several indica cultivars and two wild relatives. We have constructed a high-resolution linkage map as a foundation for map-based cloning of the bph2 locus. An advanced mapping population derived from a cross of 'Tsukushibare' (a susceptible japonica cultivar) with 'Norin-PL4' (an authentic bph2-introgression line) was used. Segregation analysis by the mass seedling test showed that bph2 behaved as a single dominant gene. Through bulked segregant analysis and linkage analysis, bph2 was located within a 3.2-cM region containing eight AFLP markers. One marker (KAM4) showed complete co-segregation with bph2, and bph2 was mapped within a 1.0-cM region delimited by KAM3 and KAM5, two flanking markers. KAM4 was converted into a PCR-based sequence-tagged-site (STS) marker and its co-segregation with bph2 was validated.

    2001年, Theoretical and Applied Genetics, 103 (4), 526 - 532

    [査読有り]

    研究論文(学術雑誌)

  • Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12

    K. Murata, M. Fujiwara, H. Murai, S. Takumi, N. Mori, C. Nakamura

    A dominant gene Bph9 of rice (Oryza sativa L.) confers resistance against a serious insect pest, brown planthopper (BPH; Nilaparvata lugens Stål). To map the Bph9 locus, a susceptible japonica breeding line 'Norin-PL9' was crossed as a seed parent with an indica cultivar 'Pokkali', which is a Bph9-carrier. Due to high sterility in the F1 and F2 progenies, genotypes for BPH response of F2 individuals were estimated indirectly by the phenotypes of F3:4 families in BPH bioassay. Segregation of BPH resistance in 62 F2 individuals did not deviate from the single dominant gene ratio. Seven RFLP and two RAPD markers were selected in the bulked segregant analysis, all of which showed normal segregation in the mapping population. Bph9 was mapped on the long arm of rice chromosome 12.

    2001年, Cereal Research Communications, 29 (3-4), 245 - 250

    [査読有り]

    研究論文(学術雑誌)

  • Sergei Tsvetanov, Ryoko Ohno, Kanako Tsuda, Shigeo Takumi, Naoki Mori, Atanas Atanassov, Chiharu Nakamura

    A cDNA library was constructed from a cold-acclimated winter-hardy common wheat (Triticum aestivum L.) cultivar 'Mironovska 808'. Using this library and a cold- and light-responsive barley cDNA clone cor14b as a probe, cDNAs of a homologous wheat gene wcor14 were isolated. Two identical cDNAs designated as wcor14a had an open reading frame encoding an acidic (pI = 4.71) and hydrophobic polypeptide with 140 amino acids (MW = 13.5 kDa). The deduced WCOR14a polypeptide showed 70% identity with the barley chloroplast- imported COR14b and had a nearly identical N-terminal, putative chloroplast transit peptide of 51 amino acid residues. Another cDNA clone wcor14b was assumed to encode a polypeptide WCORb which had 5 substitutions and a frame shift in the C-terminal region as compared with WCOR14a. RACE PCR, genomic PCR and Southern blot analyses suggested that wcor14 and its related sequences constitute a small multigene family with and without an intron in the hexaploid wheat genome. Northern blot analysis showed that transcripts of wcor14 accumulated within 3-6 hours of cold acclimation at 4°C and the level reached a maximum at day 3. The transcripts became non-detectable within 3 hours after de-acclimation at room temperature. Contrary to the barley cor14b, a similar level of wcor14 transcripts was detected under the continuous darkness. Neither treatment with NaCl, ABA nor dehydration induced its expression. Based on these results we conclude that wcor14 is a wheat orthologue of the barley cor14b and specifically induced by low temperature.

    2000年02月, Genes and Genetic Systems, 75 (1), 49 - 57

    [査読有り]

    研究論文(学術雑誌)

  • Yasunari Ogihara, Kazuhiro Isono, Toshio Kojima, Akira Endo, Mitsumasa Hanaoka, Takashi Shiina, Toru Terachi, Shigeko Utsugi, Minoru Murata, Naoki Mori, Shigeo Takumi, Kazuho Ikeo, Takashi Gojobori, Rika Murai, Koji Murai, Yoshihiro Matsuoka, Yukari Ohnishi, Hikaru Tajiri, Koichiro Tsunewaki

    Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.

    2000年, Plant Molecular Biology Reporter, 18 (3), 243 - 253

    [査読有り]

    研究論文(学術雑誌)

  • Naoko Tsukamoto, Nobuaki Asakura, Nobuaki Hattori, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

    Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal sequences in six regions encompassing 11 structural genes in all the NC hybrids. PCR-RFLP analysis showed that one region (a nad3-orf156 region) consisted of a mixture of the maternal, paternal and novel paternal-like sequences. The presence of unexpectedly high levels of the paternal sequences was confirmed by random PCR cloning and sequencing of this region. PCR-RFLP analysis of the random clones further showed that the relative stoichiometry of the maternal and paternal sequences varied depending on the plasmons from the maternal parents and the nuclear backgrounds of the paternal parents. Our results suggest that the differential amplification of the paternal mtDNA sequences is under the control of NC interaction in these NC hybrids.

    2000年, Current Genetics, 38 (4), 208 - 217

    [査読有り]

    研究論文(学術雑誌)

  • S. Ando, S. Takumi, Y. Ueda, T. Ueda, N. Mori, C. Nakamura

    Heterotrimeric GTP-binding proteins (G-proteins) play important roles in signal transduction pathways in eukaryotic cells. Through differential screening of a hairy root cDNA library of tobacco (Nicotiana tabacum L.) against transcripts from non-root tissues of normal cuttings, we obtained a partial cDNA clone that showed abundant expression and high homology to the α subunit gene of plant G-protein. After RACE-PCR, a full-length cDNA clone was obtained, which was 1,677-bp in length and contained an open reading frame encoding a protein of 384 amino acids. A cDNA clone encoding a β subunit of G-protein was also isolated from the same cDNA library based on PCR amplification and library screening. The clone was 1,600-bp in length and contained an open reading frame encoding 377 amino acids. The deduced amino acid sequences of these clones showed high homology (75.5 to 99.8% amino acid identity) with α and β subunits of other plant G-proteins. Genomic Southern blot analysis showed that the amphidiploid tobacco genome possessed two major copies of both α and β subunit genes and some minor homologous copies. Northern blot analysis showed that the transcript of α subunit gene was abundant in the root tissues, particularly in the hairy root tissues. In contrast, the level of expression of the β subunit gene was equivalent in all the tissues studied. Possible function of tobacco G-protein was discussed.

    2000年, Genes and Genetic Systems, 75 (4), 211 - 221

    [査読有り]

    研究論文(学術雑誌)

  • Kanako Tsuda, Sergei Tsvetanov, Shigeo Takumi, Naoki Mori, Atanas Atanassov, Chiharu Nakamura

    A Cor (cold-responsive) cDNA that belongs to the group-3 Lea (late embryogenesis abundant)/Rab (responsive to abscisic acid, ABA) family was isolated from a winter-hardy cultivar of common wheat (Triticum aestivum L.). Screening of a cold-acclimated cDNA library was performed using an ABA- and other stress-responsive barley cDNA clone, Hva1, as a probe. A wheat cDNA clone (designated as Wrab19) putatively encoded a basic (pI=10.3) and hydrophobic protein with 179 amino acids. The deduced protein showed characteristics of the group-3 LEA/RAB protein family. In contrast to the single copy barley Hva1, Wrab19 belonged to a multigene family in the hexaploid wheat genome and six loci were assigned to the homoeologous group 1 chromosomes. Using Wrab19 as a probe, four homologous cDNAs (designated as Wrab17) were isolated that encoded acidic (pI=4.6-4.7) and hydrophobic proteins, all with 166 amino acids. The deduced proteins showed high homology (a mean of 84% identity) with a barley gibberellic acid (GA 3)-inducible protein, ES2A, and several other group-3 LEA/RAB proteins. Wrab17 was considered to be a three-copy gene and each copy was assigned to chromosome 5A, 4B or 4D of hexaploid wheat. Transcripts of both Wrab19 and Wrab17 accumulated within 1 day of cold acclimation at 4°C. They were responsive to ABA and/or GA 3, but showed some cultivar differences in their response to these plant hormones. We conclude that the two genes are new members of the group-3 Lea/Rab-related Cor gene family in wheat.

    2000年, Genes and Genetic Systems, 75 (4), 179 - 188

    [査読有り]

    研究論文(学術雑誌)

  • Mapping of QTLs for low temperature response in seedlings of rice (Oryza sativa L.)

    S Misawa, N Mori, S Takumi, S Yoshida, C Nakamura

    A bioassay condition was developed to evaluate the low temperature response of rice (Oryza sativa L.) seedlings. Under this condition, an indica cultivar 'Hokuriku 142' did not survive. while a japonica cultivar 'Hyogo-Kitanishiki' showed 100% survival. A cross was made between the two cultivars to obtain F-2 and F-3 progenies. A frame map with 61 RFLP markers was constructed using 69 F(2)s, in which the segregation of all the markers was normal. Mean responses to the low temperature treatment of individual F(2)s were scored based on a 1-5 scale rating using F(3)s. A normal distribution was obtained showing that the low temperature response at the seedling stage was controlled by polygenes. Thirteen QTLs associated with the: low temperature response were detected on the rice chromosomes 1, 3, 9 and ii by the single point analysis. All QTLs except for 2 on chromosome 9 were with the effect in the same directions to those expected from the parental responses. The interval analysis revealed four QTLs on chromosomes 1, 3 and 11. The QTL-locating chromosomes 3, 9 and 11 show synteny with chromosome 5 of wheat and 7 (5H) of barley carrying genes for freezing tolerance.

    CEREAL RES INST, 2000年, CEREAL RESEARCH COMMUNICATIONS, 28 (1-2), 33 - 40, 英語

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    研究論文(学術雑誌)

  • Naoko Yokouchi, Nobuaki Asakura, Sergei Tsvetanov, Naoki Mori, Atanas Atanassov, Chiharu Nakamura

    Variations in the gliadin composition in the endosperm were studied in R4 somaclonal lines that were derived from immature embryo callus cultures of durum wheat (Triticum durum Desf.). Electrophoregrams of gliadin polypeptides were analyzed in 10 somaclonal lines of cv. 'Zagorka' and seven lines of cv. 'Progress' and compared with the two parental cultivars. The analysis showed that the parental cultivars were both. composed of individuals with different genotypes. Among the somaclonal lines, very high frequencies of variants which showed polypeptide patterns different from those of the parental cultivars were detected. The variations were characterized by the appearance and/or disappearance of particular polypeptides. Some variations were fixed in homozygous conditions but in a majority of somaclonal lines the variations remained in heterozygous conditions.

    Japanese Society for Plant Cell and Molecular Biology, 1999年, Plant Biotechnology, 16 (2), 167 - 170, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Segregation distortion through female gametophytes in interspecific hybrids of tetraploid wheat as revealed by RAPD analysis

    Mizuwa Manabe, Tomoko Ino, Masao Kasaya, Shigeo Takumi, Naoki Mori, Ichiro Ohtsuka, Chiharu Nakamura

    Interspecific crosses were among five tetraploid wheat species including three endemic species in Transcaucasia to study chromosone marker segregation. A reciprocal cross between Triticum turgidum and T. timopheevi produced sterile F1s, while three reciprocal F1s obtained from cross combinations of T. durum/T. persicum, T. durum/T. paleocolchicum were fertile and produced euploid F2 progeny after self fertilization. Twenty RAPD markers were identified as nuclear markers specific to one or two species by determining the genotypes of the three fertile reciprocal F1s, and six were assigned to individual chromosomes using the D genome chromosome substitution lines. Segregation analysis in the F2s showed that five among the 20 nuclear markers were almost completely or preferentially transmitted from single parents. A complete transmission through the female gametes of one T. durum/T. persicum. This complete segregation distortion through the female gametes was correlated with markedly reduced selfed seed set in the F1s. Preferential transmission of a T. paleocolchicum marker associated with the cytoplasm was also detected in a cross of T. paleocolchicum x T. turgidum.

    1999年, Hereditas, 131 (1), 47 - 53

    [査読有り]

    研究論文(学術雑誌)

  • Ac-mediated trans-activation of the Ds element in rice (Oryza sativa L.) cells as revealed by GUS assay

    Renando Solis, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

    A method using particle bombardment and β-glucuronidase (GUS) assay was applied to rice callus for detecting the excision of the maize Ds element trans-activated by the Ac transposase source. Excision of Ds biolistically introduced into rice callus resulted in the restoration of the interrupted gus gene expression, allowing visual detection of trans-activation two days after bombardment. Only the transgenic callus lines expressing the Ac transposase gene and the wild-type callus co-transformed with Ac and Ds revealed GUS activity. Frequency of excision, estimated based on the relative GUS activity, ranged from 0.3% to 2.2%. Callus lines showing different levels of Ac transcripts revealed varying excision frequencies. At the later stages of callus growth after selection for the Ac/Ds transformed lines, excision events were detected by GUS assay and confirmed by PCR and sequence analyses of the excision sites in individual colonies. GUS activity was also demonstrated in the primary regenerants from the Ac/Ds-transformed callus colonies. The method described in this study may be used as an approach for rapid detection of excision events and assessment of various factors limiting Ac/Ds activity in rice cells.

    1999年, Hereditas, 131 (1), 23 - 31

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    研究論文(学術雑誌)

  • Variations in the maize Ac transposase transcript level and the Ds excision frequency in transgenic wheat callus lines

    Shigeo Takumi, Koji Murai, Naoki Mori, Chiharu Nakamura

    To investigate the excision of a maize transposable element in wheat cells, plasmid DNAs containing a Dissociation (Ds) element located between a rice actin 1 gene promoter and a β-glucuronidase (GUS) gene (gus) were introduced into wheat callus lines by microprojectile bombardment, and transient GUS expression was assayed. The gus-expressing cells after Ds excision were detected only when the Activator (Ac) transposase gene was cotransformed. To further examine a relationship between the amount of Ac mRNA and the Ds excision frequency, the Ds-containing plasmids were introduced into 15 independent transgenic callus lines transformed with the Ac transposase gene. Ten lines expressed the Ac transposase gene under the control of either the cauliflower mosaic virus 35S promoter or the Ac native promoter. The gus gene expression that indicated the Ds excision was observed only in the transgenic callus lines stably expressing the Ac transposase gene. The number of blue spots reflecting the frequency of Ds excision was variable among them. Northern-blot analysis also showed a large variability in the amount of Ac transposase transcripts among the lines. It was however noted that the excision frequency was decreased at a high level of the Ac transposase transcripts, supporting the hypothesis that Ds excision is inhibited above a certain level of the Ac transposase as observed in maize and transgenic tobacco.

    1999年, Genome, 42 (6), 1234 - 1241

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    研究論文(学術雑誌)

  • Kazumasa Murata, Manabu Fujiwara, Chukichi Kaneda, Shigeo Takumi, Naoki Mori, Chiharu Nakamura

    A rice gene bph2 for resistance against brown planthopper (BPH), Nilaparvata lugens Stal, was reported to be recessive and either allelic or closely linked to a dominant BPH resistance gene, Bph1. bph2 was introgressed from an indica resistance donor variety, 'IR1154-243', into a japonica breeding line, 'Norin-PL4'. A segregation analysis of BPH resistance in F2 and F3 progenies from a cross of a japonica susceptible variety, 'Tsukushibare', and 'Norin-PL4', however, showed that the resistance gene in 'Norin-PL4' behaved as a dominant gene. Genotyping of 'Norin-PL4' using 99 RFLP markers covering all 12 rice chromosomes showed that 'Norin-PL4' possessed a large segment of chromosome 12 introgressed from 'IR1154243'. Six RFLP markers on the introgressed segment was cosegregated with BPH resistance and bph2 was mapped at 3.5 cM from the closest RFLP marker, G2140. The position of bph2 on the standard 'Nipponbare'/'Kasalath' map was at a considerable distance (about 30 cM) from that of Bph1 previously mapped using a different population. Despite this, no susceptible recombinants were obtained in a large number of F3 progeny from crosses between two Bph1 carrier lines and 'Norin-PL4'. Problems of dominance/recessiveness and no recombinations between the two loci were discussed.

    1998年12月, Genes and Genetic Systems, 73 (6), 359 - 364

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    研究論文(学術雑誌)

  • Renando Solis, Shinya Yoshida, Naoki Mori, Chiharu Nakamura

    Following a few reports on indica rice transformation, we demonstrated the recovery of transgenic indica rice cultivars carrying potentially useful genes through co-transformation with a selectable marker using a particle bombardment method (biolistic process). Scutellum-derived calli and embryos of 3 selected indica rice cultivars were used as explants. Co-transformation with selectable bar gene was employed to isolate transformed callus lines that are resistant to the herbicide bialaphos. Subsequently, bialaphos-resistant calli were regenerated and 29% of R0 plants were shown to possess Ac and Ds by PCR analysis. About 50% of the putative R0 transformants showed transmission of the transgene based on PCR and Southern blot analyses, indicating that a large number of primary transformants represented chimeric individuals, or that elimination of the transgenes eventually occurred during meiosis/mitosis. PCR-positive R0 plants were self-pollinated to produce R1 lines. Single and multiple integrative events were detected and R1 progenies from 3 transgenic lines exhibited the expected Mendelian inheritance pattern. Although a few rearranged copies of the transgenes were noted, a majority of the copies remained intact after integration. Expression of the CaMV35S-driven transposase gene was revealed by the presence of the transposase transcript in Northern blots. The procedure described here provides an applicable tranformation system that can be further improved to generate more Ac and Ds lines towards establishing a functional transposon mutagenesis system in indica rice.

    Japanese Society for Plant Cell and Molecular Biology, 1998年, Plant Biotechnology, 15 (2), 87 - 93, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naoki Mori, Takahiro Moriguchi, Chiharu Nakamura

    To study the phylogeny and domestication of tetraploid wheat species, variations in nuclear DNA of the cultivated and wild species were investigated by RFLP analysis. Twenty-two accessions representing 11 species of cultivated tetraploid wheat (Emmer wheat and Timopheevi wheat), 16 accessions of wild Emmer wheat (Triticum dicoccoides Korn.), 14 accessions of wild Timopheevi wheat (T. araraticum Jakubz.), and an accession of common wheat (T. aestivum) were analyzed, using 29 combinations of two restriction enzymes and 20 probes. Based on this result, the genetic distances (d) between all pairs of accessions were estimated. An average d was 0.0189 in the Emmer group and 0.0024 in the Timopheevi group, while that between the groups was 0.0698. Cluster analysis using UPGMA, NJ (neighbor joining) and maximum parsimony method showed clear differentiation of Emmer wheat and Timopheevi wheat. Among the cultivated Emmer wheat T. dicoccum Schubl. showed the largest nucleotide diversity (π = 0.0180) which was close to that (0.0186) in the wild ancestral species, T. dicoccoides. All the cultivated species, except for T. dicoccum and T. paleocolchicum Men., were grouped into a distinct cluster in the phylogenetic trees. All but one accessions of T. dicoccoides were grouped in another. The large genetic diversity in T. dicoccum, the non-free threshing species, supports the archeological evidence that T. dicoccum was the earliest domesticated tetraploid wheat.

    1997年06月, Genes and Genetic Systems, 72 (3), 153 - 161, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Variation in coxII intron in the wild ancestral species of wheat

    Naoki Mori, Naohiko T. Miyashita, Toru Terachi, Chiharu Nakamura

    To study the maternal lineage and evolution of polyploid species of wheat, variation in mitochondrial DNA was investigated in Triticum and Aegilops by PCR-aided RFLP analysis. A 1.3 kb region containing the intron of coxII was studied using 20 accessions from five species of Sitopsis section of Aegilops, one species of Einkorn wheat, four species of tetraploid wheat, and one species of common wheat. Only three restriction site changes and a single deletion/insertion were found among 884 restriction fragments surveyed. This fact suggests the highly conserved nature of this region within Triticum and Aegilops. Four haplo-types were recognized in coxII intron. A parsimonious relationship indicated that three haplo-types were independently derived from one prototype which was found in wild Einkorn and Aegilops species except for Ae. speltoides. All but one accession of Ae. speltoides possessed a derivative haplo-type, common in Timopheevi wheat. The result supported the hypothesis that Ae. speltoides donated the G genome to Timopheevi wheat however did not agree with that Ae. speltoides was the B genome donor to the Emmer and common wheat.

    1997年, Hereditas, 126 (3), 281 - 288, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chukichi Kaneda, Masato Umikawa, M. Rohinikumar Singh, Chiharu Nakamura, Naoki Mori

    The Manipur State of India is considered as a part of the center of diversity of Asian rice. To obtain information on the genetic diversity and subspecies differentiation in local rice cultivars from the area, a survey was made of selected morphological and physiological traits, chloroplast DNA (ctDNA) type, and total genomic DNA polymorphism. Judging from the discriminant score z, 32 out of 48 tested local cultivars were classified into indica, 5 into japonica, and 11 intermediate. An analysis of ORF100 of ctDNA by PCR identified 29 cultivars as deletion type (indica), and 22 as non-deletion type (japonica). Random amplified polymorphic DNA analysis revealed a high degree of polymorphism in the 51 cultivars, and 4 major clusters were recognized in a dendrogram constructed by UPGMA: 29 cultivars formed a cluster with typical indica standards, 10 cultivars formed a cluster with typical japonica standards, and 12 cultivars forming two clusters were classified as intermediate. Cultivars from hill districts were highly polymorphic in combination of the three criteria. Many indica-type cultivars had japonica ctDNA but none with the reverse combination, suggesting the compatibility of japonica cytoplasm with indica nucleus and introgression of indica to japonica in this area.

    Japanese Society of Breeding, 1996年, Breeding Science, 46 (2), 159 - 166, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tohru Suzuki, Chiharu Nakamura, Naoki Mori, Chukichi Kaneda

    An alloplasmic hybrid (nucleus-cytoplasm hybrid) of common wheat (Triticum aestivum) with a cytoplasm of wheatgrass (Agropyron trichophorum) shows highly depressed vigor and complete male sterility. The presence of one short-arm telocentric homeologous group 1 chromosome (telosome) of the cytoplasm donor, however, restores normal vigor and male fertility of the hybrid. To study role(s) of the telosome on vigor/fertility restoration, mitochondrial genome organization and gene expression were compared among seedlings of the alloplasmic line showing depressed vigor, the corresponding restored line having a pair of the telosomes, and a euplasmic nuclear donor as control. No differences were detected in the mitochondrial genome structure between the depressed line and the restored line. Northern blot analysis using ten mitochondrial genes as probes showed no differences in transcript size and number between the depressed and restored lines, although clear differences were found in size of the major transcripts of two genes (cob and orf25) between the alloplasmic lines and the euplasmic control. Steady-state transcript levels were higher in the depressed line than in the other lines for all the mitochondrial genes analyzed including rrn18& 5 when the same amount of mitochondrial RNA was loaded. The amount of rrn18& 5 transcript in the total cellular RNA, however, did not differ among the lines. Run-on transcription analysis demonstrated markedly elevated transcriptional activities of all the mitochondrial genes analyzed in the depressed line based on unit amount of mitochondrial DNA, RNA and protein. The presence of Agropyron telosomes apparently normalized the level of mitochondrial transcription. These observations suggest either direct or indirect association of the observed mitochondrial gene overexpression with the depressed vigor and male sterility of the alloplasmic hybrid. © 1995 Kluwer Academic Publishers.

    Kluwer Academic Publishers, 1995年02月, Plant Molecular Biology, 27 (3), 553 - 565, 英語

    [査読有り]

    研究論文(学術雑誌)

  • N. Mori, Y. G. Liu, K. Tsunewaki

    Intra- and inter-specific variations in the nuclear DNA of Triticum dicoccoides Körn. (2n = 28, genome constitution AABB) and T. araraticum Jakubz. (2n = 28, AAGG), wild species, respectively, of the Emmer and Timopheevi group, were studied by restriction fragment length polymorphism (RFLP) analysis. Total DNAs of 32 T. dicoccoides and 24 T. araraticum accessions, collected from throughout the distribution areas of these species, were treated with two 6-bp cutters and hybridized with 30 nuclear DNA clones as probes to detect RFLPs. A total of 167 hybrid bands were observed per accession. All the enzyme-probe combinations showed RFLPs between accessions. The average genetic distance between the T. dicoccoides accessions was 0.0135 ± 0.0031 and that between the T. araraticum accessions 0.0036 ± 0.0015, indicative of about a four-fold intraspecific variation in T. dicoccoides as compared to T. araraticum in terms of genetic distance. No significant genetic differentiation was found for the geographical populations of these species, the genetic distance between the two species being 0.0482 ± 0.0022. The interspecific divergence corrected for intraspecific divergence was 0.0395, about three times that for T. dicoccoides and 11 times that for T. araraticum. The results show that in the wild state the Emmer and Timopheevi groups are clearly differentiated and that T. dicoccoides has much greater variation than T. araraticum, suggesting a relatively recent origin for the latter and therefore a diphyletic origin for these species. © 1995 Springer-Verlag.

    Springer-Verlag, 1995年01月, Theoretical and Applied Genetics, 90 (1), 129 - 134, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naohiko Kodo, Chiharu Nakamura, Naoki Mori, Chukichi Kaneda, Hajime Kato, Takumi Yoshizawa

    Mitochondrial DNAs (mtDNAs) were purified by CsCl/bisbenzimide density-gradient ultracentrifugation from 21 strains of seven Fusarium species that cause fusarium head blight and mycotoxin contamination in wheat and other cereals. A partial PstI clone bank, from which one of twelve Pstl fragments (14.7 kb) is missing, was constructed using mtDNA from strain KU-1615 of F. graminearum. Molecular sizes of mtDNAs of single representative strains from the seven species were determined after single-, double- and triple-digestion by four or five restriction enzymes (BamHI, MluI, PstI, PvuII and XhoI), while those of others were after single-digestion by BamHI and/or PstI. MtDNA size varied from the smallest 49 kb in one strain of F. avenaceum to the largest 116 kb in one strain of F. culmorum. Restriction fragment length polymorphism (RFLP) analysis revealed a large interspecific variation, thus all the species were identified by their restriction fragment patterns and assigned to individual clusters except for F. tricinctum in that a strain studied showed identical patterns to one of two strains of F. sporotrichioides. Considerable intraspecific variation including size variation was also detected. These results indicated a high incidence of insertions/deletions both between and within species. On the basis of results obtained by the cluster analysis, some aspects of taxonomy in these Fusarium species were discussed. © 1995, The Genetics Society of Japan. All rights reserved.

    1995年, Japanese Journal of Genetics, 70 (3), 435 - 451, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chiharu Nakamura, Katsuki Ohtani, Naoki Mori, Chukichi Kaneda, Ivan Panayotov

    Chloroplast DNAs (cpDNAs) of Agropyron glaucum, Ag. trichophorum and Haynaldia villosa were studied using alloplasmic hybrids of common wheat (Triticum aestivum nuclear donor) with cytoplasms of the respective species. Chloroplast genome sizes of the two Agropyron species and Hy. villosa were very close to 135 kb of common wheat. Restriction maps were constructed using seven restriction enzymes. Eight fragment length mutations (deletions/insertions) and five recognition site mutations were detected among 167 sites studied. CpDNAs of Agropyron, Hy. villosa and common wheat were very closely related: Base substitution rate per 100 base pairs (d) was 0.10 between Hy. villosa and common wheat, 0.19 between common wheat and Agropyron, and 0.29 between Agropyron and Hy. villosa. There was only one site difference (HindIII site) between the two Agropyron species (d=0.05). © 1994, The Genetics Society of Japan. All rights reserved.

    1994年, Japanese Journal of Genetics, 69 (6), 645 - 655, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tohru Suzuki, Chiharu Nakamura, Naoki Mori, Yoko Iwasa, Chukichi Kaneda

    Short-arm telocentric chromosomes of two Agropyron species, A. glaucum and A. trichophorum, confer nucleus-cytoplasmic compatibility on the alloplasmic common wheat with cytoplasms of these Agropyron species. To determine the homoeologous group of the telocentric Agropyron chromosomes, we studied 1) chromosome pairing in a series of double monosomic-monotelosomic addition plants carrying each one of the two telocentric chromosomes and a single chromosome derived from the closely related species, A. elongatum, 2) in situ hybridization pattern with 18S.26S rDNA in alloplasmic, ditelosomic addition lines, and 3) functional compensation in the alloplasmic, monosomic A. elongatum chromosome addition plants based on plant vigor and selfed seed fertility. Our results showed that the telocentric Agropyron chromosomes belong to the homoeologous group 1. © 1994, The Genetics Society of Japan. All rights reserved.

    1994年, Japanese Journal of Genetics, 69 (1), 41 - 51, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Z. Atanassov, C. Nakamura, N. Mori, C. Kaneda, H. Kato, Jin Yin Zhe, T. Yoshizawa, K. Murai

    Canadian Science Publishing, 1994年, Canadian Journal of Botany, 72 (2), 161 - 167, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T. Ishii, T. Terachi, N. Mori, K. Tsunewaki

    Restriction fragment length polymorphisms of chloroplast (ct), mitochondrial (mt) and nuclear DNA were investigated using eight cultivars of Oryza sativa and two cultivars of O. glaberrima. Relative variability in the nuclear and cytoplasmic genomes was estimated by a common measure, genetic distance. Based on the average genetic distances among ten cultivars for each genome, the evolutionary variabilities of the mitochondrial and nuclear genomes were found to be almost the same, whereas the variability of the chloroplast genome was less than half that of the other two genomes. Cluster analyses on ct and mt DNA variations revealed that chloroplast and mitochondrial genomes were conservative within a taxon and that their differentiations were well-paralleled with respect to each other. For nuclear DNA variation, an array of different degrees of differentiation was observed in O. sativa, in contrast with little variation in O. glaberrima. As a whole, differentiation between O. sativa and O. glaberrima was clearly observed in all three genomes. In O. sativa, no notable difference was found between the cultivars 'Japonica' and 'Javanica', whereas a large differentiation was noticed between 'Japonica' (including 'Javanica') and 'Indica'. In all three genomes, the average genetic distances within 'Indica' were much larger than those within 'Japonica' (including 'Javanica'), and almost similar between 'Japonica' (including 'Javanica') and 'Indica'. These facts indicate that differentiation in O. sativa was due mainly to 'Indica'. © 1993 Springer-Verlag.

    Springer-Verlag, 1993年03月, Theoretical and Applied Genetics, 86 (1), 88 - 96, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Naoki Mori, Koichiro Tsunewaki

    Distributions of the Net and Ne2 alleles for type 1 hybrid necrosis, and Chi allele for type 1 hybrid chlorosis in two wild tetraploid wheats, Triticum dicoccoides (wiki emmer wheat) and T. araraticum (wild timopheevi wheat), have been investigated by crossing the collections of these species from their entire distribution areas with their appropriate testers. The frequencies of the Nel and Chi alleles were 53% ami 13%, respectively, in T. dicoccoides whereas they were 69 and 0% in T. araraticum. The Ne2 allele was not found in both species. The Nel allele of T. dicoccoides was mainly Nelm, while that of T. araraticum was mainly Nel*. The frequency of the Chi allele differed significantly between the two species. These facts indicate that the emmer and timopheevi groups of tetraploid wheat had been differentiated genetically before their domestication. On the frequencies of the Nel and Chi alleles, Israeli T. © 1992, The Genetics Society of Japan. All rights reserved.

    1992年, Japanese Journal of Genetics, 67 (5), 371 - 380, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T. Suzuki, C. Nakamura, H. Sakagami, N. Mori, I. Panayotov, C. Kaneda

    1992年, Japanese Journal of Genetics, 67 (2), 133 - 145, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koichiro Tsunewaki, Shinji Yamada, Naoki Mori

    A semi-wild wheat, Triticum aestivum ssp. tibetanum, collected in Tibet and taxonomically classified by Shao et al. (1980), was investigated for its phylogenetic relationship to common wheat. Ssp. tibetanum is a hexaploid spelt wheat showing wedge-type disarticulation of the ear, and it is assumed to be somewhat unstable genetically. F1 hybrids between ssp. tibetanum and other common wheats are fully fertile in both sexes, although three interchanges exist between them. The haploid genotype for hybrid necrosis and hybrid chlorosis is nelne2chlch2 the Ch2 allele, common in both Aegilops squarrosa and common wheat, including Chinese cultivars, is missing. The chloroplast genome is similar, and the mitochondrial genome is only slightly different from their counterparts in common wheats. Its cytoplasm does not induce male sterility in the all common wheat genotypes studied. These facts suggest that it is an offtype of Tibetan common wheat. © 1990, The Genetics Society of Japan. All rights reserved.

    1990年, The Japanese Journal of Genetics, 65 (5), 353 - 365, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yao Guang Liu, Koichiro Tsunewaki, Naoki Mori

    To develop detailed linkage maps of restriction fragment length polymorphism (RFLP) sites in wheat chromosomes, it was necessary to construct a genomic DNA library and to characterize the clones obtained. Forty-nine per cent of the clones were of single or low copy number per genome. With 91 clones of this class, as probes, and with two to four restriction endonucleases, for DNA digestion, RFLPs were examined among eight common wheats and a single emmer wheat. About 20% of the probes, and 13% of the probe-enzyme combinations revealed genetic polymorphism among the common wheats. DNA deletions account for most of the genetic differences among these wheat genomes. Based on the RFLP data, phylogenetic distances among the nine polyploid wheats were estimated, and a dendrogram showing the genetic relationships among them was constructed. © 1990, The Genetics Society of Japan. All rights reserved.

    1990年, The Japanese Journal of Genetics, 65 (5), 367 - 380, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • Genetic effect of the Aegilops caudata plasmon on manifestation of the Ae. cylindrica genome

    Koichiro Tsunewaki, Naoki Mori, Tatsuya Yotsumoto, Shigeo Takumi

    GENETICS SOC JAPAN, 2014年12月, GENES & GENETIC SYSTEMS, 89 (6), 305 - 305, 英語

    研究発表ペーパー・要旨(国際会議)

  • Conventional and molecular studies on brown planthopper (Nilaparvata lugens Stal) resistance genes in rice: a basis for future study of natural insect resistance genes using molecular markers in Nepal

    Prem N. Sharma, Naoki Mori, Shigeo Takumi, Chiharu Nakamura

    Rice productivity greatly affected by various biotic and abiotic stresses. Insect pest are one of the major biotic constraints to cause significant losses in rice production. Brown plant hopper (BPH), Nilaparvata rugense Stal, is the most serious insect-pest of rice in Asia where most of the world rice is produced. Controlling insects using chemicals is already proven detrim

    2014年07月, Nepal Journal of Science and Technology, 15 (1), 145 - 156, 英語

    記事・総説・解説・論説等(学術雑誌)

  • Returning the Aegilops caudata plasmon to its home after half a century of its departure: reconstitution of Ae. caudata by reuniting its genome with the plasmon that coexisted with the common wheat genome for 50 generations

    Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

    GENETICS SOC JAPAN, 2011年12月, GENES & GENETIC SYSTEMS, 86 (6), 411 - 411, 英語

    研究発表ペーパー・要旨(国際会議)

  • Production and characterization of Aegilops cylindrica having the plasmon of Ae. caudata

    Koichiro Tsunewaki, Naoki Mori, Shigeo Takumi

    GENETICS SOC JAPAN, 2009年12月, GENES & GENETIC SYSTEMS, 84 (6), 465 - 465, 英語

    研究発表ペーパー・要旨(国際会議)

  • Possible horizontal transfer of Mutator-like elements within Poaceae

    Nobuaki Asakura, Naoki Mori, Shinya Yoshida, Ichiro Ohtsuka, Chiharu Nakamura

    GENETICS SOC JAPAN, 2007年12月, GENES & GENETIC SYSTEMS, 82 (6), 526 - 526, 英語

    研究発表ペーパー・要旨(国際会議)

  • Mitochondrial microsatellite variability in the ancestral species of wheat (Aegilops speltoides Tausch.)

    Yoshiaki Ohmichi, Takashige Ishii, Chiharu Nakamura, Naoki Mori

    GENETICS SOC JAPAN, 2007年12月, GENES & GENETIC SYSTEMS, 82 (6), 538 - 538, 英語

    研究発表ペーパー・要旨(国際会議)

  • S Takumi, T Kosugi, K Murai, N Mori, C Nakamura

    The plant knotted1 (kn1)-like homeobox genes are known to play important roles in the maintenance of shoot apical meristem (SAM), determination of cell fate and differentiation of vegetative tissues. To study structural diversity of the three homoeologous loci encoding a KN1-like homeobox protein in the hexaploid wheat genome, we isolated clones from a cDNA library of young spikes of Japanese common wheat cultivar 'Norin 26'. Three different but highly homoeologous cDNAs were isolated and their sequences were determined. The mean homology of the deduced amino acid sequences was 96% as compared to the barley ortholog KNOX3. The wheat kn1-like homeobox proteins named WKNOX1 are encoded by a single set of homoeologous genes on the homoeologous group 4 chromosomes in the three component genomes of common wheat, i.e. 4A, 4B and 4D. The nucleotide sequence data and the Southern blot pattern suggested that the three homoeologous loci of wknox1 genes are highly conserved through polyploid evolution of wheat. They were expressed in SAM-containing shoots and young spikes but not in developed leaves, glumes and lemmas and callus tissues. The ectopic expression of the wknox1 was observed in lemma of wheat Hooded (Hd) mutants. The result suggested that the Hd gene is a dominant allele of the wknox1 locus on chromosome 4A. (C) 2000 Elsevier Science B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2000年05月, GENE, 249 (1-2), 171 - 181, 英語

  • S Takumi, K Murai, N Mori, C Nakamura

    To develop a transposon tagging system in wheat (Triticum aestivum L.), transgenic wheat lines containing a transposase gene of the maize Activator (Ac) element were produced and characterized. The Ac transposase gene under the control of a cauliflower mosaic virus 35S promoter was introduced into cultured wheat embryos by particle bombardment. Several transgenic wheat plants expressing the transposase gene were independently recovered. Southern- and Northern-blot analyses of their progeny showed that the expression of the Ac transposase gene was stably inherited, and three fixed Ac lines were established. By RT-PCR analysis, products from fully spliced transcripts of the Ac element were confirmed. Cultured embryos isolated from the stable Ac lines were further bombarded with plasmids having a maize Dissociation (Ds) element located between a rice Act1 promoter and a beta-glucuronidase (gus) gene, and transient gus expression was observed after the Ds excision. These findings suggest that the maize Ac transposase gene is precisely processed and an active transposase protein is synthesized in the transgenic Ac lines. The Ds element is trans-activated and excised in wheat cells by the action of the Ac transposase gene.

    SPRINGER VERLAG, 1999年05月, THEORETICAL AND APPLIED GENETICS, 98 (6-7), 947 - 953, 英語

  • Molecular variation in chloroplast DNA regions in ancestral species of wheat

    N. T. Miyashita, N. Mori, K. Tsunewaki

    Restriction map variation in two 5-6-kb chloroplast DNA regions of five diploid Aegilops species in the section Sitopsis and two wild tetraploid wheats, T riticum dicoccoides and T riticum araraticum, was investigated with a battery of four-cutter restriction enzymes. A single accession each of T riticum durum, T riticum limopheevi and T riticum aestivum was included as a reference. More than 250 restriction sites were scored, of which only seven sites were found polymorphic in Aegilops speltoides. No restriction site polymorphisms were detected in all of the other diploid and tetraploid species. In addition, six insertion/deletion polymorphisms were detected, but they were mostly unique or species-specific. Estimated nucleotide diversity was 0.0006 for A. speltoides, and 0.0000 for all the other investigated species. In A. speltoides, none of Tajima's D values was significant, indicating no clear deviation from the neutrality of molecular polymorphisms. Significant non-random association was detected for three combinations out of 10 possible pairs between polymorphic restriction sites in A. speltoides. Phylogenetic relationship among all the plastotypes (plastid genotype) suggested the diphyletic origin of T. dicoccoides and T. araraticum. A plastotype of one A. speltoides accession was identical to the major type of T. araraticum (T. timopheevi inclusively). Three of the plastotypes found in the Sitopsis species are very similar, but not identical, to that of T. dicoccoides, T. durum and T. aestivum.

    1994年, Genetics, 137 (3), 883 - 889, 英語

    書評論文,書評,文献紹介等

書籍等出版物

  • エッセンシャル遺伝学・ゲノム科学(原著第7版)

    Hartl, Daniel L, 中村, 千春, 岡田, 清孝, 森,直樹

    共訳, 第14章, 化学同人, 2021年01月, 日本語, ISBN: 9784759820485

  • インダス 南アジア基層世界を探る(長田俊樹 編), 第11章 インド冬作穀類の起源と変遷

    大田 正次, 森 直樹

    その他, 京都大学学術出版会, 2013年10月, 日本語, 紀元前2600年頃からインド亜大陸北西部で栄えたインダス文明は、紀元前1900年頃に衰退したことが知られている。しかし、なぜ衰退したのかについてはいまだに不明である。地球環境学研究所では、当時のインダス地域における人類社会と自然環境を復元することにより、その衰退原因を明らかにする研究(プロジェクト代表者:長田俊樹)が行われた。筆者らは、このプロジェクトの一員として現代インドに残る伝統的な農業体系とその中に数千年にわたって継続的に栽培されてきた在来コムギについて現地調査を行い、当時の農耕システムの復元を試みたので、その成果を第11章に紹介した。, ISBN: 9784876983001

    学術書

  • インダス 南アジア基層世界を探る(長田俊樹 編), コラム 「それなら知っているよ Gund godiだよ。」ーインド矮性コムギ再発見の日ー

    森 直樹

    その他, 京都大学学術出版会, 2013年10月, 日本語, ISBN: 9784876983001

    学術書

  • 麦の自然史(佐藤洋一郎、加藤鎌司 編)

    森 直樹

    共著, 北海道大学出版会, 2010年03月, 日本語, 人が麦を栽培し始めてから現在までの人と麦の関係をテーマに、麦類の由来、歴史、細胞遺伝学、進化学、考古学、民族植物学、文化人類学といった幅広い視点から栽培植物としての麦類について記されたものである。筆者は第3章「染色体数の倍加により進化したコムギ」を担当, ISBN: 9784832981904

    学術書

講演・口頭発表等

  • 野生エンマーコムギにおけるVRN-A3座の変異と栽培コムギにおける分布

    西村 和紗, 森 直樹, 半田 裕一, 川浦 香奈子, 北島 宣, 中﨑 鉄也

    日本育種学会第139回講演会, 2021年03月20日, 日本語

    ポスター発表

  • コムギ倍数性進化における雑種ゲノム倍加の重要性:交雑実験とQTL解析の結果から

    松岡 由浩, 森 直樹

    第15回ムギ類研究会, 2020年12月26日, 日本語

    ポスター発表

  • 野生エンマーコムギと栽培エンマーコムギの穂と穎果の形態形質の比較

    岩﨑 菖子, 森 直樹

    第15回ムギ類研究会, 2020年12月26日, 日本語

    ポスター発表

  • 野生エンマ―コムギの遺伝的背景を持つ戻し交雑自殖系統を用いた草型の解析

    粕淵 星也, 島田 沙織, 牛 恒一, Vladutu Cristian, Kianian Shahryar, 森 直樹

    第15回ムギ類研究会, 2020年12月26日, 日本語

    ポスター発表

  • コムギの細胞質置換系統におけるミトコンドリアDNAの分子内組み換えの定量的解析

    鎌田 知希, 太田 星史, 牧田 真之, 辻村 真衣, 寺地 徹, 森 直樹

    第15回ムギ類研究会, 2020年12月26日, 日本語

    ポスター発表

  • パンコムギのミトコンドリアゲノム上に散在するショートリピートを介した組換えに関する研究

    辻村真衣, 佐藤南美, 永島伊都子, 森直樹, 寺地徹

    日本育種学会第137回講演会, 2020年03月28日, 日本語

    口頭発表(一般)

  • エンマ―コムギのF3集団を用いた小穂内の頴果サイズと種子休眠性の遺伝学的解析

    福山智章, 森直樹, 大田正次

    第14回ムギ類研究会, 2019年11月02日

    ポスター発表

  • エンマーコムギの戻し交雑自殖系統群による穂の形態形質の遺伝学的解析

    山口雄平, 宮崎裕貴, 森直樹

    第14回ムギ類研究会, 2019年11月02日

    ポスター発表

  • コムギの細胞質置換系統におけるミトコンドリアDNAの分子内組み換えの定量的解析

    鎌田知希, 太田星史, 牧田真之, 辻村真衣, 寺地徹, 森直樹

    第14回ムギ類研究会, 2019年11月02日

    ポスター発表

  • 一粒系コムギにおける小穂あたりの着粒数と種子休眠性の遺伝学的解析

    松井寧々, 森直樹, 大田正次

    第14回ムギ類研究会, 2019年11月02日

    ポスター発表

  • 野生エンマ―コムギの遺伝的背景を持つ戻し交雑自殖系統を用いた草型の解析

    粕淵 星也, 島田 沙織, 牛 恒一, Vladutu Cristian, Kianian Shahryar, 森 直樹

    第14回ムギ類研究会, 2019年11月02日

    ポスター発表

  • ムギの近縁野生種Aegilops geniculataの種内の変異と遺伝的分化

    吉村優介, 森直樹, 大田正次

    第14回ムギ類研究会, 2019年11月02日

    ポスター発表

  • タルホコムギとの交雑親和性およびタルホコムギとのF1雑種の自殖着粒率からみた二粒系コムギのナチュラルバリエーション

    松岡由浩, 森直樹

    第14回ムギ類研究科, 2019年11月02日

    ポスター発表

  • トルコ南部の野生二倍体コムギ集団における非休眠個体頻度の多様性

    大田 正次, 森 直樹, Ozkan Hakan

    日本育種学会第135回講演会, 2019年03月, 日本語, 日本育種学会, 千葉大学 西千葉キャンパス, 演者らはこれまで野生二倍性コムギの1種である野生一粒系コムギの種子休眠性について、①休眠性の個体が同一小穂の第1小花に小さくて休眠性、第2小花に大きくて非休眠性の穎果を着ける、②第2小花に比べて第1小花の穎果が小さい個体ほど強い休眠性をもつ、③小穂内に単独で着いた穎果は着粒した小花に係らず大きくて非休眠性である、④野生型の中に栽培型と同じように非休眠性で第1小花に大きな穎果を着ける個体があること、を明らかにしてきた。本研究では、野生二倍性コムギ集団の発芽適応戦略と栽培コムギ集団の成立過程を明らかにする目的で、栽培化起源地の野生二倍性コムギ集団における非休眠性個体の頻度を調査した。 【材料と方法】トルコ南部の野生二倍性コムギ集団で1穂/個体を採集しチュクロワ大学で保存しているサンプルから実験集団を育成するために、11集団について1粒/穂を抽出し、201, 国内会議

    口頭発表(一般)

  • 一粒系コムギにおける小穂あたりの着粒数と種子休眠性の遺伝学的解析

    松井 寧々, 森 直樹, 大田 正次

    第13回 ムギ類研究会, 2018年11月, 日本語, ムギ類研究会, 横浜市立大学 木原生物学研究所, 一粒系コムギには、小穂あたり種子が1粒着粒する傾向が高いものと2粒着粒する傾向が高いものがあり、系統間で変異がみられる。小穂あたり2粒着粒する野生一粒系コムギでは、同一小穂内の2つの穎果のうち、第1小花に着粒した穎果は休眠性をもち、またサイズが小さい。この形質は栽培型では消失している。本研究では、これらの形質についてその遺伝様式を知るためにQTL解析を行っている。, 国内会議

    ポスター発表

  • トルコ南部の野生四倍性コムギ集団における野生二粒系コムギと野生チモフェービ系コムギの分布

    大田 正次, 森 直樹, Hakan Ozkan

    第13回ムギ類研究会, 2018年11月, 日本語, 横浜市立大学 木原生物学研究所, 四倍性コムギには二粒系コムギとチモフェービ系コムギが含まれる。両種が自生するトルコ南部で収集した集団サンプルを用いて集団内・集団間の多様性を分析しているが、両種の野生型亜種の穂の形態は酷似し、穂のサンプルから両者を区別することは困難である。今回、チュクロワ大学で比較栽培した11集団442個体について葉面の毛の形質により両種を同定し集団レベルでの分布を明らかにした。本研究はJSPS科研費 JP16K07559, JP17K07272の助成を受けている。, 国内会議

    ポスター発表

  • コムギの近縁野生種 Aegilops geniculata の種内変異

    吉村優介, 森 直樹, 大田正次

    第13回ムギ類研究会, 2018年11月, 日本語, 横浜市立大学 木原生物学研究所, コムギの近縁野生種Aegilops geniculata Rothは、種内に大きな形態的変異がみられ、細胞遺伝学的および遺伝学的にも大きな変異が含まれている。本研究では、まず、葉緑体ゲノムのマイクロサテライト座の多型を調査し、種内の系統関係や遺伝的分化の様相を明らかにする。これまでの解析結果から、亜種内、変種内にも大きな変異が存在することが判明した。また、出穂・開花時期などの生理的形質にも種内変異がみられた。, 国内会議

    ポスター発表

  • エンマ―コムギのF3集団を用いた小穂内の頴果サイズと種子休眠性の遺伝学的解析

    福山智章, 森 直樹, 大田正次

    第13回 ムギ類研究会, 2018年11月, 日本語, 横浜市立大学 木原生物学研究所, 野生エンマ―コムギでは多くの場合、第1小花に着粒する頴果の休眠性が深く、また、第2小花の頴果と比べ、第1小花の頴果は小さくて軽い。本研究では、これらの形質の生理学的な調査と関与するQTLの位置の推定を行っている。発芽試験の結果、野生エンマ―コムギの第1小花の頴果の約50%は約半年で休眠性を失うことがわかった。また、4B染色体に頴果の大きさの比に関するQTLが存在する可能性が示唆された。, 国内会議

    ポスター発表

  • エンマーコムギの戻し交雑自殖系統群における小穂に占める穎果の割合

    山口 雄平, 宮崎 裕貴, 森 直樹

    第13回 ムギ類研究会, 2018年11月, 日本語, ムギ類研究会, 横浜市立大学 木原生物学研究所, 〇・・森直樹(神戸大・農学研究科) ◦, N. Mori (Grad. Sch. Agr. Sci., Kobe U.) これまでに、野生エンマーコムギ (Triticum turgidum ssp. dicoccoides) と栽培エンマーコムギ (Triticum turgidum ssp. dicoccum) を用いて育成された92の戻し交雑自殖系統を用い、野生種と栽培種の間で大きく異なる性質について2反復で栽培して継続的に調査してきた。また、この戻し交雑自殖系統についてSSRマーカーの遺伝子型を決定し、栽培化関連QTLの位置や効果を解析してきた。今回は小穂に占める穎果の割合と穎果の形態に関する形質について主に調査したので報告する。, 国内会議

    ポスター発表

  • トルコ南部の野生四倍性コムギ集団における非休眠性個体頻度の多様性

    大田 正次, 森 直樹, Ozkan Hakan

    一般社団法人日本育種学会 第133回講演会, 2018年03月, 日本語, 九州大学, トルコ南部の栽培化起源地域の11集団で収集し、チュクロワ大学で保存している穂のサンプルから1穂あたり1粒を抽出した約400個体の野生四倍性コムギの実験集団を、2014年~2015年と2016年~2017年にチュクロワ大学で比較栽培し、それぞれ2015年秋と2017年秋に種子休眠性の程度とそれに相関した形態形質の変異を調査した。その結果、自然集団にはさまざまな頻度で非休眠性の個体が含まれていることが明らかとなり、また、生育環境の良否によって集団中の非休眠性種子の頻度が変動することが示唆された。これらの結果から、野生四倍性コムギ集団の自然環境下における発芽適応戦略と栽培コムギ集団の成立過程について考察した。, 国内会議

    口頭発表(一般)

  • スペルトコムギ (Triticum aestivum L. ssp. spelta) 系統KT19-1 が保有する新規Vrn-A3座アレルの早生化効果

    西村 和紗, 齊藤 隆成, 半田 裕一, 森 直樹, 川浦 香奈子, 北島 宣, 中崎 鉄也

    一般社団法人日本育種学会 第133回講演会, 2018年03月, 日本語, 九州大学, 【緒言】本邦のコムギ栽培において、登熟期が梅雨の時期と重なり穂発芽などの雨害が発生し、収量および品質の低下が問題となっているため、早生化が重要な育種目標である。これまでの研究で、四倍体コムギ系統TN26(T. turgidum L. ssp. dicoccum)がプロモーター領域に特異的な挿入配列を持つ新規Vrn-A3早生アレル(挿入型Vrn-A3)を保有することを見出した。一方、多様な六倍体コムギ系統を用いて、挿入型Vrn-A3の分布を調査したところ、ドイツ原産のスペルトコムギ系統(T. aestivum L. ssp. spelta)がTN26と同様の挿入型アレルを保有することが明らかとなった。本研究では、挿入型Vrn-A3を保有するスペルトコムギ系統KT19-1と普通コムギ系統CSとの交雑RILsを用いて、KT19-1が保有するVrn-A3挿入, 国内会議

    ポスター発表

  • 六倍体コムギ系統 (Triticum aestivum L.) におけるTN26型Vrn-A3早生アレルの分布

    西村 和紗, 齊藤 隆成, 森 直樹, 半田 裕一, 北島 宣, 中崎 鉄也

    第12回ムギ類研究会, 2017年12月, 日本語, 京都大学農学部, 本研究では、四倍体コムギ系統TN26 (T. turgidum L. ssp. dicoccum) が保有するVrn-A3早生アレルの六倍体コムギにおける分布を解析した。日本品種のミニコアコレクション (Kobayashi et al. 2016) であるJWC96系統を用いた解析の結果、早生アレルを保有する系統は北海道や東北における15品種に見られた。次に、Hirosawa et al. (2004) の供試材料85系統を用いた解析の結果、ドイツ原産のスペルトコムギ系統が早生アレルを保有することが明らかとなった。これらの結果から、このアレルの活用が六倍体品種の早生化育種に有効である可能性を見出した。, 国内会議

    ポスター発表

  • 野生二粒系コムギの形態および遺伝的変異から見た種内の多様性と地理的分化

    村川 夏貴, 森 直樹, Özkan Hakan, 大田 正次

    第12回ムギ類研究会, 2017年12月, 日本語, 京都大学農学部, 自然分布域をほぼカバーするように採集された57系統の野生二粒系コムギを使用して、形質調査とSSRマーカーによるミトコンドリアDNAの解析を行った。調査の結果、形態的形質について本種は種内に大きな多様性を持っており、いくつかの形質については採集された地域によって大きな偏りがあることがわかった。また、現在解析中のミトコンドリアゲノムには、これまでのところほとんど変異が見られないことがわかった。, 国内会議

    ポスター発表

  • 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統による栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Cristian, Kianian Shahryar, 森 直樹

    第12回ムギ類研究会, 2017年12月, 日本語, 京都大学農学部, エンマーコムギは最も初期に栽培化されたコムギといわれている。本研究では、エンマーコムギの栽培化の初期に起こった遺伝的変化の解明を目的として、野生エンマーコムギと栽培エンマーコムギから作出された野生種の遺伝的背景を持つ戻し交雑自殖系統について、それらの栽培化関連形質を調査した。また、分子マーカーによるQTL解析を行うことで、エンマーコムギの栽培化関連形質を支配するQTLの染色体上の位置や効果を推定した。, 国内会議

    ポスター発表

  • 野生エンマーコムギにおける小穂内の穎果サイズの違いと種子休眠性に関与するQTL の解析にむけて

    寺田 凪沙, 森 直樹, 大田 正次

    第12回ムギ類研究会, 2017年12月, 日本語, 京都大学農学部, 野生エンマーコムギの小穂では、第一小花と第二小花に着粒する穎果の大きさや休眠性に種内変異があり、第一小花に着粒する穎果が第二小花に比べて小さい場合、その穎果の休眠性が深くなる傾向がある。栽培エンマーコムギではこの傾向がほとんどない。本研究では、この穎果サイズや休眠性の小穂内二型性に関与するQTLを同定するため、野生エンマーコムギと栽培エンマーコムギのF2及びF3系統を用いて連鎖地図の作成を行っている。, 国内会議

    ポスター発表

  • コムギのミトコンドリアゲノムにおける分子内組換えの定量的解析

    太田 星史, 牧田 真之, 辻村 真衣, 寺地 徹, 森 直樹

    第12回ムギ類研究会, 2017年12月, 日本語, 京都大学農学部, ミトコンドリアはほとんど全ての真核生物に見られる細胞小器官であり、エネギー生産という重要な役割を担っている。コムギのミトコンドリアは独立した環状のDNA分子をもち、さらにこの分子内の組換えで生じた小環状分子の存在が報告されている。この小環状分子の動態はミトコンドリアの機能と関わりを持つ可能性が有るため、本研究ではコムギにおけるミトコンドリアゲノムの分子内組換え頻度の定量的解析系の確立を試みている。, 国内会議

    ポスター発表

  • Genetic diversity between and within two natural populations of wild emmer wheat (Triticum turgidum subsp. dicoccoides) in southern Turkey: insights from chloroplast DNA fingerprinting

    Naoki Mori, Takako Shizuka, Hakan Ozkan, Shoji Ohta

    8th International Triticeae Symposium 2017, 2017年06月, 英語, Wernigerode/Gatersleben, Germany, Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the ancestral species of cultivated tetraploid wheat with AABB genomes (i.e. emmer wheat: T. turgidum ssp. dicoccum, durum wheat: T. turgidum ssp. turgidum conv. durum and etc.). Wild emmer wheat is an annual, predominantly self-pollinated species. Because of its full interfertility with domesticated emmer wheat, this w, 国際会議

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  • Genetic diversity between and within natural populations of wild emmer wheat (Triticum turgidum subsp. dicoccoides) in southern Turkey: Insights from nuclear-SSR and morphological variation

    Esra Çakır, Ahmad Alsaleh, Naoki Mori, Shoji Ohta, Hakan Ozkan

    8th International Triticeae Symposium 2017, 2017年06月, 英語, Wernigerode/Gatersleben, Germany, Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the ancestral species of cultivated tetraploid wheat with AABB genomes. Because of its full interfertility with domesticated emmer wheat, this wild species can serve as one of the most important genetic resources to improve durum as well as bread wheat. To clarify the magnitude of genetic diversity between and within pop, 国際会議

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  • A Japan-Turkey cooperative research project on genetic diversity in wild wheat and their genetic change during domestication process

    Shoji Ohta, Naoki Mori, Hakan Ozkan

    8th International Triticeae Symposium 2017, 2017年06月, 英語, Wernigerode/Gatersleben, Germany, Southeastern Turkey is located at the heart of the Fertile Crescent in Southwest Asia. It is one of the centers of genetic diversity in wild wheat, wild barley and wild relatives of Mediterranean-Southwest Asian legumes, such as lentils, chick peas, peas, and so on, and is also one of the probable candidates where einkorn and emmer wheat were domesticated from their wild proge, 国際会議

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  • 野生エンマーコムギの穎果サイズの小穂内二型性と種子休眠性に関わるQTL の解析にむけて

    寺田 凪沙, 大田 正次, 森 直樹

    日本育種学会第131回講演会, 2017年03月, 日本語, 名古屋大学, 【目的】 種子休眠性の喪失は種子作物の栽培化に伴って起こった顕著な遺伝的変化の一つである。演者らはこれまでに野生エンマーコムギの自然集団を調査し,同一小穂の第1小花に着粒する穎果は休眠性で小さく,第2小花の穎果は非休眠性で大きい傾向があること,また休眠性の程度と小穂内の穎果サイズの違いには相関があり第2小花に比べて第1小花の穎果が小さい個体ほど休眠が深いこと,さらに小穂内にみられるこのような二型性の程度には大きな種内変異があることを明らかにしてきた(大田,第129回講演会)。本研究の目的は,野生型にみられる種子休眠性の特性を理解し,当該形質に関与する量的形質遺伝子の単離に向けて,このQTLを連鎖地図上に位置付けることである。これらの遺伝子が単離できれば,不明な点が多い野生種の環境適応戦略についても理解が深まると考える。 【材料】 野生エンマーコム, 国内会議

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  • 二粒系コムギの栽培化による穎果へのエネルギー分配率の変化

    宮崎 裕貴, Pham-Minh Ngoc, Liberatore Katie, Kianian Shahryar, Vladutu Cristian, 森 直樹

    日本育種学会第131回講演会, 2017年03月, 日本語, 名古屋大学, 【目的】コムギは西南アジアにおいて倍数性進化と栽培化によって起源したと考えられている。約1万年前、人間は野生エンマーコムギ (Triticum turgidum ssp. dicoccoides)を利用し始めたが、その過程で栽培化が起こり、栽培エンマーコムギ (T. turgidum ssp. dicoccum)が生まれたとされている。エンマーコムギの野生種と栽培種の間では様々な形質で違いが見られるが、それらの遺伝的制御機構の全貌については不明な点が多い。本研究では、栽培化によって小穂重あたりの穎果重の割合が変化したのではないかという仮説を立て、このようなエネルギー配分の変化を遺伝学的に検証することを目的する。 【材料と方法】栽培エンマーコムギ (以下Dcm1001とする) を母本として野生エンマーコムギ (以下Dcc63とする) と交雑して得たF1, 国内会議

    ポスター発表

  • 酒米のルーツをさぐる - DNAでさぐる日本酒米の起源

    森 直樹

    橿原市昆虫館サイエンスカフェ, 2017年03月, 日本語, 橿原市昆虫館, 日本酒の製造に好適な一群のイネ品種群としてしられる酒造好適米(酒米)の起源について、DNAの解析結果から推定されることについて講演した。, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • エンマーコムギの戻し交雑自殖系統を用いた栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Cristian, 石井 尊生, Kianian Shahryar, 森 直樹

    日本育種学会第131回講演会, 2017年03月, 日本語, 名古屋大学, [目的] エンマーコムギ (Triticum turgidum ssp. dicoccum) は、コムギ属の中で最も初期に栽培化されて起源した栽培種の一つであり、初期農耕の起源と世界各地への伝播において重要な役割を果たした。本研究では、コムギ栽培化の初期に起こった遺伝的変化を解明することを目的として、祖先野生種 (T. turgidum ssp. dicoccoides) の遺伝的背景を持つ戻し交雑自殖系統を育成し、栽培化関連形質を評価することによりこれらを支配するQTLの解析を進めている。 [方法] 栽培エンマーコムギ (以下Dcm1001と呼ぶ)と野生エンマーコムギ (以下Dcc63) の交雑から野生種の遺伝的背景を持つ戻し交雑自殖系統 (BC2F14) を育成した(図1)。これらの栽培化関連形質を評価し、分子マーカーによるQTL解析により、, 国内会議

    ポスター発表

  • qPCR法を用いたコムギのミトコンドリアゲノムにおける分子内組換えの 定量的解析

    太田 星史, 牧田 真之, 辻村 真衣, 寺地 徹, 森 直樹

    日本育種学会第131回講演会, 2017年03月, 日本語, 名古屋大学, 【背景と目的】ミトコンドリアはほとんど全ての真核細胞に含まれる細胞小器官である。好気呼吸によるエネルギー生産という真核生物が生存する為に必要不可欠な役割を担っている。また, 核とは別に独自のゲノムを持っており, コムギを含む高等植物では不均一なサイズの小型環状DNA分子が複数存在する事が報告されている。このDNA分子の発生にはミトコンドリアゲノムにおける分子内組換えが関与しているとされているが, その詳細は不明な点が多い。 筆者らは,コムギにおいてミトコンドリアゲノムの動態と機能発現の関係を明らかにするため研究を進めている。その基盤として,ミトコンドリアゲノムの分子内組換えを正確に検出し,その頻度を定量する実験系の確立を目指している。これによって,ミトコンドリアゲノムの動態が成長段階や組織によって異なるのかどうかを調査できるものと考えている。 【方, 国内会議

    ポスター発表

  • 野生二粒系コムギにおける小穂内の穎果サイズの違いと種子休眠性に関与するQTL の解析にむけて

    寺田 凪沙, 森 直樹, 大田 正次

    第11回ムギ類研究会, 2016年12月, 日本語, 岡山大学 資源植物学研究所, 野生二粒系コムギの小穂では、第一小花と第二小花に着粒する穎果の大きさや休眠性に種内変異があり、第一小花に着粒する穎果が第二小花に比べて小さい場合、その穎果の休眠性が深くなる傾向がある。栽培二粒系コムギではこのような現象がみられない。本研究では、穎果サイズや休眠性の二形性に関与するQTLを解析するため、野生二粒系コムギと栽培二粒系コムギのF1植物に由来するF2及びF3系統を用いて連鎖地図の作成を行っている。, 国内会議

    ポスター発表

  • 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Cristian, 石井 尊生, Kianian Shahryar, 森 直樹

    第11回ムギ類研究会, 2016年12月, 日本語, 岡山大学 資源植物学研究所, エンマーコムギはコムギ属の中で最も初期に栽培化されたコムギの一つとされている。本研究では、エンマーコムギの栽培化の初期に起こった遺伝的変化の解明を目的として、野生エンマーコムギと栽培エンマーコムギから作出された野生種の遺伝的背景を持つ戻し交雑自殖系統について、それらの栽培化関連形質を調査した。また、分子マーカーによるQTL解析を行うことで、エンマーコムギの栽培化関連形質を支配するQTLの染色体上の位置や効果を推定した。, 国内会議

    ポスター発表

  • 二粒系コムギの栽培化に伴って変化した穎果の形態と重量の遺伝学的解析

    宮崎 裕貴, Pham Minh Ngoc, Liberatore Katie, Kianian Shahryar, 森 直樹

    第11回ムギ類研究会, 2016年12月, 日本語, 岡山大学 資源植物学研究所, 栽培二粒系コムギと野生二粒系コムギを親として育成した戻し交雑自殖系統92系統を用いて、穎果の大きさや形について形質調査を行った。また、210のSSRマーカーと9321のSNPマーカーの遺伝子型に基づいて分散分析を行った。形質調査の結果、各形質の中で穎果の幅が粒重の増加と最も強い正の相関を示した。また、穎果が丸くなることと粒重の増加には正の相関があった。栽培化による粒重の増加には幅の増加、また穎果の形の変化が大きく影響したのではないかと考えられる。, 国内会議

    ポスター発表

  • コムギのミトコンドリアゲノムにおける分子内組換えの定量的解析

    太田星史, 牧田真之, 辻村真衣, 寺地徹, 森 直樹

    第11回ムギ類研究会, 2016年12月, 日本語, 岡山大学 資源植物学研究所, ミトコンドリアはほとんど全ての真核生物に見られる細胞小器官であり、エネギー生産という重要な役割を担っている。コムギのミトコンドリアは独立した環状のDNA分子をもち、さらにこの分子内の組換えで生じた小環状分子の存在が報告されている。この小環状分子の動態はミトコンドリアの機能と関わりを持つ可能性が有るため、本研究ではコムギにおけるミトコンドリアゲノムの分子内組換え頻度の定量的解析系の確立を試みている。, 国内会議

    ポスター発表

  • 二粒系コムギの栽培化に伴って変化した頴果の大きさや形態の遺伝学的解析

    宮崎 裕貴, Pham Minh Ngoc, Liberatore Katie, Kianian Shahryar, 森 直樹

    近畿作物育種研究会 第182回例会, 2016年11月, 日本語, 近畿大学生物理工学部, <はじめに> コムギは西南アジアにおいて倍数性進化と栽培化によって起源したと考えられている。約1万年前、人間は野生エンマーコムギ (Triticum turgidum ssp. dicoccoides)を利用し始めたが、その過程で栽培化が起こり、栽培エンマーコムギ (T. turgidum ssp. dicoccum)が生まれたとされている。栽培化により脱粒性の喪失、頴果の重量の増加などが起こった。その結果、コムギの収穫効率や収量は向上し、農耕の発展に大きく寄与したと思われる。エンマーコムギの野生種と栽培種の間では脱粒性以外にも多くの形質で違いが見られるが、それらの遺伝的制御機構の全貌については不明な点が多い。我々は、エンマーコムギの栽培化関連形質に関与するQTLの同定を目的として研究を進めている。今回は、頴果の大きさや形に関与するQTLの位置や効果, 国内会議

    口頭発表(一般)

  • 非休眠性個体を高頻度で含む野生四倍性コムギ集団の探索-栽培コムギ集団の成立をめぐって

    大田 正次, 森 直樹, Ozkan Hakan

    日本育種学会第129回講演会, 2016年03月, 日本語, 横浜市立大学, 種子非休眠性の栽培コムギ集団の成立過程を解明する目的で、栽培化起源地域であるトルコ南部の自然集団10集団で収集した野生四倍性コムギ(集団あたり約50個体)を、2014年11月から2015年6月にチュクロワ大学で比較栽培した後、2015年11月末から12月初に各個体の穎果の重さと発芽率および発芽指数を調査した。その結果、自然集団には栽培型と同様に小穂内に大きさの揃った穎果を着ける非休眠性の個体が存在し、そのような個体を高頻度で含む集団があることが明らかとなった。この結果から、栽培化に伴う栽培コムギ集団の成立について考察した。, 国内会議

    口頭発表(一般)

  • 酒米のルーツをさぐる − DNAでさぐる日本酒米の起源

    森 直樹

    橿原昆虫館 サイエンスカフェ, 2016年03月, 日本語, 奈良県立橿原昆虫館, 橿原昆虫館, 日本酒は古くから我国の伝統的な技術と材料を用いて作られてきた。酒造工程で用いられる酒米はできあがる酒の品質を大きく左右するため、よりよい酒米品種を追求する先人たちによって「意識的」にあるいは「無意識のうちに」選ばれてきた一群のイネ品種群であると考えられるが、その起源はよくわかっていない。全国各地に伝わる「酒米品種」のもつ遺伝的多様性とルーツを明らかにするため、兵庫県立農林水産技術総合センター・酒米試験地と共同研究を行い、全国各地の酒米についてそれらのDNAにどれくらいの違いがあるのか調査した。その結果、食用米と比べて酒米の遺伝的多様性は小さく、祖先に当たる品種の数もごく限られたものであった可能性が高いということ、また、酒米はもともと関西地方で生まれ、その後全国各地に拡がったのではないかということが明らかになった。, 国内会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • スペルタコムギが持つVIIb型ミトコンドリアゲノムの解析

    辻村 真衣, 森 直樹, 寺地 徹

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, Bゲノムを持つ倍数性コムギには2タイプのミトコンドリアゲノム(VIIa型とVIIb型)が存在し、両者はHindIIIによる1つの制限断片で明瞭に区別される。これまでの解析から、VIIb型には約9.5kbpの特異的な配列が存在することがわかっている(Tsujimura et al. 2013)。 本研究ではVIIb型ミトコンドリアゲノムの全貌を明らかにするため、NGSを用いて全ゲノムの解読を行った。また、既報のVIIa型と比較して、SNPsの検出を行い、両者の違いを明確にすることを試みた。, 国内会議

    口頭発表(一般)

  • 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Christian, 石井 尊生, Kianian Shahryar, 森 直樹

    第10回 ムギ類研究会, 2015年12月, 日本語, 三重大学農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連形質の解析を行った。その結果、まだメーカー数が藤生部であるが穂軸の脆弱性や1穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, 国内会議

    ポスター発表

  • 栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連QTLの解析

    宮崎 裕貴, Pham-Minh Ngoc, Vladutu Christian, 石井 尊生, Kianian Shahryar, 森 直樹

    第10回 ムギ類研究会, 2015年12月, 日本語, 三重大学 農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連QTLの解析を行った。その結果、穂軸の脆弱性や1穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, 国内会議

    ポスター発表

  • コムギのミトコンドリアゲノムの分子内組換えの定量的解析

    太田 星史, 牧田 真之, 辻村 真衣, 寺地 徹, 森 直樹

    第10回 ムギ類研究会, 2015年12月, 日本語, ムギ類研究会, 三重大学 農学部, コムギのミトコンドリアゲノムには、大小様々な反復配列が存在し、それらの間で分子内組換えが起こることによって小環状分子種が生じていることがしられている。しかし、分子内組換えの頻度や組織ごとの分子数についてはほとんどわかっていない。本研究ではqPCR法によるこれら組換え分子の量的解析系の開発を試みた。, 国内会議

    ポスター発表

  • 野生二粒系コムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統群の作出:栽培化関連形質の遺伝学的解析に向けて

    森 直樹, 牛 恒一, Vladutu Cristian, 石井 尊生, Kianian Shahryar

    日本育種学会第128回講演会, 2015年09月, 日本語, 新潟大学, エンマーコムギ(Triticum turgidum ssp. dicoccum)は今から約1万年前に野生二粒系コムギ(別名パレスチナコムギ、T. turgidum ssp. dicoccoides)から栽培化されて誕生した。このコムギは新石器時代から数千年にわたってムギ農耕の主要な作物として利用されたことが知られている。我々はこの栽培化の過程でゲノムにどのような変化が起こったのかを明らかにするため、これまでに栽培種と野生種の交雑に由来するF2集団や栽培種の遺伝的背景に野生種の染色体断片を導入した戻し交雑系統群を用いて解析してきた。今回、栽培化初期の遺伝的変化を捉えることを目的として、野生二粒系コムギの遺伝的背景に栽培エンマーコムギの染色体断片を導入した戻し交雑系統群を作成したので報告する。, 国内会議

    ポスター発表

  • 栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の遺伝学的解析

    宮崎 裕貴, Pham-Minh Ngoc, Vladutu Cristian, 石井 尊生, Kianian Shahryar, 森 直樹

    日本育種学会第128回講演会, 2015年09月, 日本語, 新潟大学, 約1万年前、人間は野生エンマーコムギ (Triticum turgidum ssp. dicoccoides)を利用し始めたが、その過程で栽培化が起こり、栽培エンマーコムギ (T. turgidum ssp. dicoccum)が生まれた。エンマーコムギの野生種と栽培種の間では脱粒性以外にも多くの形質で違いが見られるが、それらの遺伝的制御機構の全貌については不明な点が多い。本研究では、エンマーコムギの栽培化関連形質に関与するQTLの同定を目的としてエンマーコムギの遺伝的背景に野生エンマーコムギの染色体断片を導入した戻し交雑自殖系統を作出し、DNAマーカーによるQTL解析を行った。その結果、いくつかの栽培化関連形質に関与するQTLについてその位置と効果の大きさを推定できた。, 国内会議

    ポスター発表

  • トルコ南部に位置する二つの自然集団における野生二粒系コムギ(Triticum turgidum ssp. dicoccoides) の葉緑体ゲノムの多様性

    森 直樹, 静 貴子, Hakan Ozkan, 大田 正次

    日本育種学会 第127回講演会, 2015年03月, 日本語, 多摩川大学, 野生二粒系コムギ(Triticum turgidum ssp. dicoccoides) は栽培二粒系コムギの祖先野生種であり、コムギの育種において重要な遺伝資源である。この野生種は西南アジアのいわゆる肥沃な三日月地帯に分布しており、トルコ南部はこのコムギが栽培化された地域として注目されている。我々は過去10年以上にわたりトルコ南部の野生二粒系コムギを調査してきた。このコムギの自然集団内の遺伝的変異と遺伝的構造を明らかにするため、トルコ南部の2集団を選び、それぞれの集団内を6から8の区画に分けて、それぞれの区画から採集したサンプルを用いて葉緑体ゲノムのマイクロサテライト座の変異を調査した。この結果、トルコ南部の野生二粒系コムギは集団内に非常に高い変異を保有しているが、その変異の空間的分布には大きな偏りがあることが判明した。, 国内会議

    口頭発表(一般)

  • 戻し交雑自殖系統を用いたエンマーコムギの栽培化関連形質の遺伝学的解析

    森 直樹, Ngoc Pham Minh, Vladutu Cristian, Thanh Pham Thien, Thanh Phan Thi, 石井 尊生, Kianian Shahryar

    日本育種学会 第126回講演会, 2014年09月, 日本語, 南九州大学, エンマーコムギ (Triticum turgidum ssp. dicoccum)は、約1万年前に野生種 (パレスチナコムギ、T. turgidum ssp. dicoccoides) から栽培化されて誕生した。このコムギは新石器時代から数千年にわたり西南アジアからヨーロッパにかけて主要な穀物として利用されたことが知られている。我々はコムギの栽培化初期の遺伝的変化を明らかにするため、エンマーコムギとパレスチナコムギのF1にエンマーコムギを戻し交雑し、エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を作成した。本講演では、この系統を用いて脱粒性などの栽培化関連形質のQTL解析を行ったので報告する。, 国内会議

    ポスター発表

  • Aegilops cylindricaゲノムの発現に対するAe. caudataプラズモンの遺伝的影響

    常脇 恒一郎, 森 直樹, 四元 達也, 宅見 薫雄

    日本遺伝学会 第86回大会, 2014年09月, 日本語, 長浜バイオ大学, 半世紀にわたりパンコムギゲノム(AABBDD)と共存したAegilops caudataプラズモンをAe. caudata (ゲノム CC)に逆移入してこの種を再構成し、ゲノムに対するプラズモンの遺伝的自律性を検証する研究を進めている。この過程で,パンコムギに共生してきたcaudataプラズモンを橋渡し的にAe. cylindrica(ゲノムCCDD)に移入し、その異種プラズモン系統を育成する必要が生じた。このAe. cylindricaの異種プラズモン系統と正常系統の13形質を5世代に亘って比較分析したところ、部分雄性不稔の誘発のみ認められた。両系統の葉緑体とミトコンドリアゲノムの46 SSR座位の比較では、23座位に差異が見られた。, 国内会議

    口頭発表(一般)

  • 4倍性コムギのミトコンドリアゲノムのタイプを変更する核ゲノム領域の特定

    辻村 真衣, 森 直樹, 山岸 博, 寺地 徹

    日本育種学会 第126回講演会, 2014年09月, 日本語, 南九州大学, Bゲノムを持つEmmer-Dinkelコムギのミトコンドリア(mt)ゲノムには、SCARマーカーで区別されるVIIa型とVIIb型がある。このうち4倍性の栽培コムギ、Triticum dicoccumの1系統(dcm1001)はVIIb型のmtゲノムを、4倍性の野生種、T. dicoccoidesの1系統(dcc63)はVIIa型のmtゲノムをそれぞれ持つ。我々はdcm1001にdcc63の花粉を交配して得たF1に、dcm1001あるいはdcc63を2回連続戻し交配し、それらを9回あるいは10回自殖させて、2つのRIL集団を作出した。これあのマーカー遺伝子型を詳細に解析したところ、6B染色体上の8.2cMの領域が、mtゲノムタイプの決定に関与していることがわかった。, 国内会議

    口頭発表(一般)

  • Genetic diversity and domestication of emmer wheat

    MORI NAOKI

    Institute seminar at Cereal Disease Laboratory, United States of America Department of Agriculture (USDA)- Agricultural Research Service (ARS), 2014年08月, 英語, Cereal Disease Laboratory, United States of America Department of Agriculture (USDA)- Agricultural Research Service (ARS), Cereal Disease Laboratory, USDA-ARS & Univ. Minnesota, St. Paul, MN, U.S.A., The domestication of wheat and barley was the most important step in the emergence of farming communities that led to the ancient civilisations of Mesopotamia. Several lines of evidence indicate that emmer wheat (Triticum turgidum subsp. dicoccum, genome: AABB, 2n = 28) was the earliest form of domesticated wheat derived from wild emmer (T. turgidum subsp. dicoccoides, genome:, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • 野生二粒系コムギの自然集団における葉緑体ゲノムの遺伝的多様性と分化

    静 貴子, 森 直樹, オズカン ハカン, 大田 正次

    近畿作物育種研究会 第177回例会, 2014年07月, 日本語, 神戸大学大学院農学研究科, 栽培二粒系コムギ(エンマーコムギ、Triticum turgidum L. ssp. dicoccum)は、約1万年前に西南アジアの「肥沃な三日月地帯」と呼ばれる地域で、野生二粒系コムギ(T. turgidum L. ssp. dicoccoides)から栽培化された。野生二粒系コムギは、現在もこの地域における一年生草本の主要な構成要素の一つである。核及び葉緑体ゲノムの解析から、二粒系コムギの栽培化は、この「肥沃な三日月地帯」の北端に位置するトルコ南部でおこった可能性が高いと考えられている。本研究では、野生二粒系コムギの自然集団内における遺伝的多様性と集団間の分化を明らかにするため、トルコ南部の地理的に近い2集団で採集した個体について葉緑体ゲノムの解析を行った。解析の結果、24座のうち集団30bで14座、31bで15座に遺伝的変異がみられた。各座のア, 国内会議

    口頭発表(一般)

  • 野生コムギの種子休眠性ー栽培化過程における種子サイズの増加に伴う休眠性の消失

    大田 正次, 森 直樹, オズカン ハカン

    日本育種学会 第125回講演会, 2014年03月, 日本語, 東北大学, 野生コムギの種子休眠性について以下のことを明らかにしてきた。1.野生コムギには種子休眠性に大きな遺伝的変異があり非休眠性の個体も存在する。2.遺伝的に休眠性をもつ野生個体の小穂に2粒着粒したとき、第1小花に休眠性の小さな穎果、第2小花に非休眠性の大きな穎果が着く。小穗に1粒着粒した穎果は非休眠性となる。従って、早く発芽した個体を選んでも非休眠性の遺伝子型は選抜できない。3.2粒着粒した小穂の第1小花の穎果が第2小花の穎果に比べて小さいほど強い休眠性をもつ。4,栽培コムギは遺伝的に非休眠性であり、第1小花に第2小花と同じ大きさ、あるいは第2小花より大きな穎果を着ける。5.穎果の大きさと休眠性は同一あるいは密接に連鎖した2つの主働遺伝子によっておもに支配されている。これらの結果から、栽培化の初期の段階では、揃った大きさの大きな穎果を着ける個体の選択が非休, 国内会議

    ポスター発表

  • 自然集団における野生二倍性コムギ及び四倍性コムギの小穂と頴果の形態的変異

    大井 紗貴, 大田 正次, 森 直樹, オズカン ハカン

    日本育種学会 第124回大会, 2013年10月, 日本語, 鹿児島大学, 種子休眠性は種子繁殖する一年生植物が環境の不意の変化に対応して集団を存続するために重要な遺伝的形質である。演者らは、これまでに野生コムギの種子休眠性について以下のことを明らかにしてきた。1) 野生一粒系および二粒系コムギでは、2粒着いた小穂の第1小花の穎果が同じ小穂の第2小花の穎果および小穂内に単独で着いた穎果に比べて有意に小さく、休眠性を示すが、他の穎果は非休眠性である。2) 自然集団において、2粒着いた小穂の第1小花の穎果は不斉一に発芽し、その多くが翌年の秋に発芽する。3) 野生コムギの個体間で種子休眠性の程度には大きな変異があり、野生一粒系コムギでは小穂あたりの着粒数が多い個体ほど、野生二粒系コムギでは第1小花の穎果が第2小花に比べて小さい個体ほど遺伝的に強い休眠性をもつ。4) 野生二粒系コムギでは、同じ小穂内に大きさの異なる穎果を着ける形質と, 国内会議

    口頭発表(一般)

  • 核ゲノム構成により違いを示すEmmer-Dinkelコムギのミトコンドリアの多型

    辻村真衣, 森 直樹, 山岸博, 寺地徹

    日本育種学会 第124回大会, 2013年10月, 日本語, 鹿児島大学, 核にBゲノムを持つEmmer-Dinkelコムギのミトコンドリア(mt)ゲノムには、11.2kbpのHindIII制限断片(H3断片)の有無により区別される、VIIa型とVIIb型の2タイプが存在する。我々はH3断片とその周辺領域の塩基配列情報からmtDNAを精製せずとも、全DNAからVIIa型とVIIb型を区別可能なSCAR(H3マーカー)を作成した(第119回育種学会)。一方、Emmer-Dinkelコムギの葉緑体ゲノムは、SSRマーカーによりplastgroup IとIIの2つに大別される (Hirosawa et al, 2004)。我々はEmmer-Dinkelコムギにおける細胞質ゲノムの分化の詳細を明らかにするため、これらのマーカーを利用して、55系統についてミトコンドリアと葉緑体のゲノムタイプを調べた。, 国内会議

    ポスター発表

  • Genetic diversity and differentiation of chloroplast genome in wild emmer wheat within and among its natural populations

    Takako hizuka, Naoki Mori, Shoji Ohta, Hakan Ozkan

    日本育種学会 第124回大会, 2013年10月, 日本語, 鹿児島大学, Southern Turkey is one of the richest regions in terms of the diversity of Triticum species. To clarify its genetic diversity and geographical differentiation, we have been surveying chloroplast genome of natural populations of wild emmer wheat (T. turgidum ssp. dicoccoides) in southern Turkey., 国内会議

    口頭発表(一般)

  • Genetic analysis of domestication traits in emmer wheat using an F2 population

    Naoki Mori, Phan Thi Thanh, Cristian Vladutu, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Shahryar Kianian

    12th International Wheat Genetics Symposium,, 2013年09月, 英語, Pacifico Yokohama, Japan, Emmer wheat (Triticum turgidum ssp. dicoccum) was a principal crop in the development and spread of Neolithic agriculture in the Old World. It represents the primitive situation in the domestication of AABB tetraploid wheat. It has non free-threshing (hulled) habit and relatively fragile rachis, although it has non-shattering spike. The more advanced forms of tetraploid whea, 国際会議

    ポスター発表

  • コムギ・エギロプス属植物のミトコンドリアゲノムの解析2. Aegilops speltoidesの細胞質を持つ置換系統のミトコンドリアゲノム

    辻村 真衣, 森 直樹, 山岸 博, 寺地 徹

    日本育種学会 第123回講演会, 2013年03月, 日本語, 東京農業大学, Ae. speltoidesの細胞質を持つ細胞質置換コムギ(核親はT. aestivum)を材料に、ミトコンドリアゲノムの解析を行った。定法によりミトコンドリアを単離した後、塩化セシウム密度勾配法でmtDNAを精製し、次世代シークエンサー(Roche 454)により塩基配列を得た。その結果、約53Mbの情報量を持つ、約109,000個の有効なリードが得られ、それらは全長の合計約548kbの44のコンティグを構成した。葉緑体DNAのコンタミネーションを除き、mtDNA由来のコンティグをマニュアルで整列化したところ、Ae. speltoidesのmtDNAとして、全長476kbの環状マスターサークルを仮定することができた。 我々はこれまで、倍数性コムギの進化に関わる植物のうち、T. aestivumミトコンドリアゲノム(Ogihara et al. 2, 国内会議

    ポスター発表

  • 葉緑体おとび核DNAの分子多型からみたインド亜大陸のエンマーコムギとインド矮性コムギの遺伝的多様性と起源

    新見 友季子, 森 直樹, 大田 正次, 千葉 一, ヴァサント シンデ, 長田 俊樹

    日本育種学会 第122回講演会, 2012年09月, 日本語, 京都産業大学, 紀元前2600年頃南アジアに興ったインダス文明では、この地域に特有の気候を巧みに利用した農業が営まれ、エンマ―コムギ(T. turgidum ssp. dicoccum)やこの地域に固有の普通系コムギであるインド矮性コムギ(T. aestivum ssp. sphaerococcum)が重要な冬作物として利用されていたと考えられている(Weber 1999)。我々は、地球環境学研究所プロジェクト「環境変化とインダス文明」の一環として、これらのコムギの起源と伝播について考察するために、葉緑体ゲノムの遺伝的多様性を探ってきた。本報告では最近の調査で新たに採集した系統を加え、核ゲノムのDNAマーカーを併用して解析したので報告する。, 国内会議

    ポスター発表

  • ゲノム特異的STSマーカーを用いたTriticum aestivum ssp. sphaerococcumの起源の解析

    朝倉 史明, 森 直樹, 中村千春, 大塚一郎

    日本育種学会 第122回講演会, 2012年09月, 日本語, 京都産業大学, 普通系コムギは二粒系コムギ(T. turgidum L.)と近縁野生種であるタルホコ ムギ(Aegilops tauschii Coss.)との交雑により成立したことが判明しているが、 いつ、どこで、何回、普通系コムギは生じたかについては未だ不明な点が多い。 これらの疑問を解明する一つの方法が、亜種の系統解析である。 Talbertら(1998)はDゲノム特異的STSマーカーであるA1座には二つのアリ ル(A型とB型)があることを見出した。Asakuraら(2011)は、インド矮性コ ムギを含む普通系コムギにおけるA1座の解析から、インド矮性コムギはA1座の A型アリルを持つパンコムギから生じた可能性が高いことを報告している。 Blakeら(2004)は、ADP glucose pyrophosphorylase大サブユニットのBゲノ ムコピー(Ag, 国内会議

    口頭発表(一般)

  • Molecular genetic analysis of the QTLs involved in the domestication of emmer wheat

    Thanh P. T, C. Vladutu, S. Kianian, T. Ishii, T. Pham, S. Nasuda, M. Nitta, N. Mori

    日本育種学会 第122回講演会, 2012年09月, 英語, 京都産業大学, Significant association between wheat and human began in southwest Asia more than 10000 years ago. Emmer wheat was one of the founder crop that was domesticated from wild emmer wheat. In the present study, QTL analysis was carried out to estimate the valuable QTLs underlying the domestication of emmer wheat., 国内会議

    口頭発表(一般)

  • Genetic diversity of wild emmer wheat in the natural populations in southern Turkey revealed by microsatellite analysis of chloroplast DNA

    Takako Shizuka, Naoki Mori, Shoji Ohta, Hakan Ozkan

    日本育種学会 第122回講演会, 2012年09月, 英語, 京都産業大学, In the Fertile Crescent, southern Turkey is one of the richest regions in terms of the diversity of Triticum and Aegilops species. To clarify its adaptation strategy to the environment, geographical differentiation and domestication, we have been surveying natural populations of wild emmer wheat in southern Turkey. Here we report the recent results on the genetic diversity of, 国内会議

    ポスター発表

  • Aegilops caudata、その細胞質をもつ細胞質置換コムギ及び両者の核と細胞質から再構成されたAe. caudataのオルガネラDNAの比較マイクロサテライト分析

    四元 達也, 森 直樹, 宅見 薫雄, 常脇 恒一郎

    日本育種学会, 2012年09月, 日本語, 京都産業大学, 国内会議

    ポスター発表

  • コムギ・エギロプス属植物のミトコンドリアゲノムの解析1.チモフェービコムギの細胞質を持つ置換系統のミトコンドリアゲノム

    辻村真衣, 森 直樹, 冨岡閲子, 寺地徹

    日本育種学会第121回講演会 宇都宮大学、3月, 2012年03月, 日本語, 日本育種学会, コムギ属植物の中でGゲノムをもつ4倍体であるチモフェービコムギのミトコンドリアゲノムの全塩基配列を解析したのでその詳細を報告した。, 国内会議

    口頭発表(一般)

  • インドで新たに採集したインド矮性コムギとエンマーコムギの葉緑体ゲノムの多様性

    新見友季子, 森 直樹, 千葉一, S. Vasant, 大田正次, 長田俊樹

    日本育種学会 第121回講演会 宇都宮大学、3月, 2012年03月, 日本語, 宇都宮大学, 紀元前2600年頃から南アジアに興ったインダス文明では、この地域に特有の機構を宅見に利用した農業が営まれ、エンマーコムギやインド矮性コムギは重要な冬作物として利用されていたと考えられる。本発表では新たにインドで採集したエンマーコムギとインド矮性コムギの遺伝的多様性を明らかにし、インド亜大陸におけるこれらの起源と伝播を考察するため、葉緑体ゲノムの変異を調査した。, 国内会議

    ポスター発表

  • Seed dormancy and germination strategy of wild diploid and tetraploid wheat revealed from the observation at their natural population in southern Turkey

    大田 正次, 森 直樹, 苅安 理恵, 山田 ちなつ, H. Ozkan

    日本育種学会 第121回講演会, 2012年03月, 日本語, 宇都宮大学, 国内会議

    口頭発表(一般)

  • Seed dormancy and germination strategy of wild diploid and tetraploid wheat revealed from the observation at their natural population in southern Turkey

    Shoji Ohta, Naoki Mori, Rie Kariyasu, Chinatsu Yamada, Hakan Ozkan

    Japanese Society of Plant Breeding, 2012年03月, 日本語, 宇都宮大学, Seed dormancy is one of the essential genetic characters for wild seed propagated plant species to maintain their populations under a variable environment. In December of 2010 and 2011, we surveyed a total of seven natural populations in Kartal Dagi region of southern Turkey to observe the germination of wild diploid and tetraploid wheat species., 国内会議

    口頭発表(一般)

  • インドにおけるエンマーコムギとインド矮性コムギの遺存的栽培と利用

    森 直樹

    ムギ類研究会, 2011年11月, 日本語, 横浜市立大学木原生物学研究所、横浜, 国内会議

    [招待有り]

    口頭発表(招待・特別)

  • 山田錦の両親系統における酒造好適性にかかわる染色体領域の推定

    八軒 雄大, 吉田 晋弥, 石井 尊生, 太田 淳, 明石 貴裕, 西村 顕, 森 直樹

    日本育種学会 第120回講演会, 2011年09月, 日本語, 福井県立大学、福井, 国内会議

    ポスター発表

  • プラズモン半世紀ぶりに古巣に帰る:パンコムギ核と50世代共生してきたAegilops caudataプラズモンの逆移入によるAe. caudataの再構成

    常脇 恒一郎, 森 直樹, 宅見 薫雄

    日本遺伝学会 第83回講演会, 2011年09月, 日本語, 京都大学,京都, 国内会議

    口頭発表(一般)

  • プラズモン半世紀ぶりに古巣に帰る:パンコムギ核と50世代共生してきたAegilops caudataプラズモンの逆移入によるAe. caudataの再構成

    常脇 恒一郎, 森 直樹, 宅見 薫雄

    日本遺伝学会, 2011年09月, 日本語, 京都大学, 国内会議

    口頭発表(一般)

  • オルガネラゲノムの変異からみたコムギ近縁野生種Aegilops umbellulata、Ae. neglecta及びAe. columnarisの多様性と系統関係

    森 直樹, 八杉 有香, 大田 正次

    日本育種学会 第120回講演会, 2011年09月, 日本語, 福井県立大学、福井, 国内会議

    口頭発表(一般)

  • インドにおけるエンマーコムギの栽培と伝統的利用

    大田 正次, 森 直樹, 千葉 一, V. Shinde, 長田 俊樹

    日本育種学会 第120回講演会, 2011年09月, 日本語, 福井県立大学、福井, 国内会議

    ポスター発表

  • The two ancient wheats, emmer wheat and Indian dwarf wheat, are still alive in India - their cultivation and utilization-

    Shoji Ohta, Naoki Mori, Hajime Chiba

    Indus Project International Symposium 2011: “Environmental Change and the Indus Civilization”, 2011年08月, 英語, Research Institute for Humanity and Nature, Kyoto, Japan, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Chloroplast DNA variation in emmer wheat and Indian dwarf wheat in Indian subcontinent

    Naoki Mori, Toshiya Takagi, Hajime Chiba, Shoji Ohta

    Indus Project International Symposium 2011: “Environmental Change and the Indus Civilization”, 2011年08月, 英語, Research Institute for Humanity and Nature, Kyoto, Japan, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 野生四倍性コムギにおける種子休眠性の小穂内二型性—トルコ東南部の自然集団における発芽調査

    大田正次, 森 直樹, H. Ozkan

    日本育種学会 第119回講演会, 2011年03月, 日本語, 横浜市立大学、横浜, 国内会議

    口頭発表(一般)

  • 倍数性コムギのミトコンドリアゲノムに見られる構造変異とその分布

    辻村 真衣, 森 直樹, 寺地 徹

    日本育種学会 第119回講演会, 2011年03月, 日本語, 横浜市立大学,横浜, 国内会議

    口頭発表(一般)

  • Rediscovery of the relict cultivation of the ancient Indus crop, Indian dwarf wheat, in southwestern India

    Naoki Mori, Shoji Ohta, Hajime Chiba, V. Shinde, M. Kajale, Toshiki Osada

    日本育種学会 第119回講演会, 2011年03月, 日本語, 横浜市立大学、横浜, 国内会議

    口頭発表(一般)

  • Genetic diversity in wheat. II.

    Naoki Mori

    Special lecture on genetic diversity of crop species, 2010年11月, 英語, Chiang Mai University, Thailand, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • Genetic diversity in wheat. I.

    Naoki Mori

    Special Lecture on genetic diversity of crop species, 2010年11月, 英語, Kasetsart University, Thailand, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • Evolution and domestication of wheat species inferred by chloroplast DNA fingerprinting. II.

    Naoki Mori

    Special Seminar on genetic diversity of crop species, 2010年11月, 英語, Chiang Mai University, Thailand, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • Evolution and domestication of wheat species inferred by chloroplast DNA fingerprinting. I.

    Naoki Mori

    Special seminar on genetic diversity of crop species, 2010年11月, 英語, Kasetsart University, Thailand, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • 葉緑体ゲノムの遺伝的変異からみたコムギ近縁野生種Aegilops umbellulataの多様性とAe. neglecta及びAe. columnarisの倍数性進化

    八杉 有香, 森 直樹, 大田 正次

    日本育種学会 第118回講演会, 2010年09月, 日本語, 秋田県立大学、秋田, 国内会議

    ポスター発表

  • 倍数性コムギの進化に新しい知見をもたらすミトコンドリアゲノムの構造変異

    辻村 真衣, 冨岡 閲子, 森 直樹, 寺地 徹

    日本育種学会第188回講演会, 2010年09月, 日本語, 秋田県立大学、秋田, ,,森直樹,(2010) . 、、9月., 国内会議

    口頭発表(一般)

  • アビシニア高地を中心とした栽培エンマーコムギの遺伝的変異

    竹中 祥太朗, 森 直樹, 河原 太八

    日本育種学会、第188回講演会, 2010年09月, 日本語, 秋田県立大学、秋田, 国内会議

    口頭発表(一般)

  • Genetic diversity of emmer wheat and Indian dwarf wheat in India inferred from chloroplast DNA analysis

    Takagi Toshiya, MORI NAOKI, Ohta Shoji, Chiba Hajime, Shinde Vasant, Kajale Mukund, Osada Toshiki

    日本育種学会 第118回講演会, 2010年09月, 英語, 秋田県立大学、 秋田, 国内会議

    ポスター発表

  • Dゲノム特異的STSマーカーA1の塩基配列からみたTriticum aestivum ssp. sphaerococcumの起源

    朝倉 史明, 森 直樹, 中村 千春, 大塚 一郎

    日本育種学会 第118回講演会, 2010年09月, 日本語, 秋田県立大学、秋田, 国内会議

    ポスター発表

  • アビシニア高地を中心とした4倍性コムギ(Triticum turgidum L.)の遺伝的変異と地域分布

    竹中 祥太朗, 森 直樹, 河原 太八

    日本育種学会第117回講演会, 2010年03月, 日本語, 京都大学、京都, 国内会議

    ポスター発表

  • 葉緑体DNAのSSR多型からみたインド在来コムギの遺伝的多様性

    高木俊哉, 森 直樹

    ムギ類研究会, 2009年11月, 日本語, 芦原、福井, 国内会議

    ポスター発表

  • コムギ近縁野生種Aegilops neglectaおよびAegilops columnarisのオルガネラゲノムの多様性と自生地における地理的変異

    八杉 有香, 森 直樹

    ムギ類研究会, 2009年11月, 日本語, 芦原、福井, 国内会議

    ポスター発表

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

    Pham Thien Thanh, Ryo Ishikawa, Naoki Mori, Takashige Ishii

    6th International Conference of Rice Genetics, 2009年11月, 英語, Manila Hotel, Philippine, 国際会議

    ポスター発表

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

    Thanh PT, Ishikawa R, Mori N, Ishii T

    International Rice Genetics Symposium, 2009年11月, 英語, Manila, Philippines, 国際会議

    ポスター発表

  • Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species

    Mori, N, Hidehira, Y, Ishii, T, Nakamura, C

    4th International Conference on Energy Efficiency and Agricultural Engineering, 2009年10月, 英語, Rousse, Bulgaria, 国際会議

    口頭発表(一般)

  • 易脱穀性二粒系コムギの葉緑体ゲノムの遺伝的変異

    高木 俊哉, 森 直樹, 河原 太八, 中村 千春

    日本育種学会 第116回講演会, 2009年09月, 日本語, 北海道大学, 国内会議

    ポスター発表

  • コムギ近縁野生種Aegilops neglectaおよびAegilops columnarisの葉緑体ゲノムの多様性と自生地における地理的変異

    八杉 有香, 森 直樹, 大田 正次

    日本育種学会 第116回講演会, 2009年09月, 日本語, 日本育種学会, 北海道大学, 国内会議

    ポスター発表

  • コムギ近縁野生種Aegilops neglecta-Ae. columnaris種複合における遺伝的変異と地理的分布 – 倍数性、形態及び雑種不稔性

    大田 正次, 藤田 裕子, 前坂 良和, 岩崎 理恵, 森 直樹, Ozkan Hakan

    日本育種学会 第116回会講演会, 2009年09月, 日本語, 北海道大学, 国内会議

    ポスター発表

  • Aegilops caudataプラズモンを持つAe. cylindricaの作出とその特性

    常脇 恒一郎, 森 直樹, 宅見 薫雄

    日本遺伝学会 第81回講演会, 2009年09月, 日本語, 信州大学, ,森直樹, (2009) . 日本遺伝学会、信州大学、9月., 国内会議

    口頭発表(一般)

  • Intraspecific variation of chloroplast DNA in Aegilops speltoides

    Naoki Mori, Hiroyuki Watatani, Takashige Ishii, Yasutaka Kondo, Taihachi Kawahara, Chiharu Nakamura

    6th International Triticeae Symposium, 2009年06月, 英語, Kyoto University, Kyoto, Japan, 国際会議

    ポスター発表

  • Allelic diversity at chloroplast microsatellite loci among polyploid wheat species

    Naoki Mori

    6th International Triticeae Symposium, 2009年06月, 英語, Kyoto University, Kyoto, Japan, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Screening of BPH resistance/infestation-associated genes by means of AMF.

    Gamalath N.S, MORI Naoki, Muhammad N, NAKAMURA Chiharu

    5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, 2005年11月, 英語, 未記入, Manila, Philippines, 国際会議

    口頭発表(一般)

  • Profiling of endosperm gene expression in Sake-brewing rice by SAGE.

    TAKAHASHI T, KAWAMURA M, YOSHIDA S, MATSUMURA H, TERAUCHI R, NAKAMURA Chiharu, MORI Naoki

    5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, 2005年11月, 英語, 未記入, Manila, Philippines, 国際会議

    口頭発表(一般)

  • Identification of simple and effective bioassay method for submergence tolerance in rice (Oryza sativa).

    Manangkil O, Vu Thi Thu H, MORI Naoki, YOSHIDA S, NAKAMURA Chiharu

    5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, 2005年11月, 英語, 未記入, Manila, Philippines, 国際会議

    口頭発表(一般)

  • Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by molecular markers.

    MORI Naoki, HASHIMOTO Z, YOSHIDA S, KAWAMURA M, ISHII T, IKEGAMI M, TAKUMI Shigeo, KAMIJIMA Osamu, NAKAMURA Chiharu

    5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, 2005年11月, 英語, 未記入, Manila, Philippines, 国際会議

    口頭発表(一般)

  • Differential responses of three brown planthopper populations to japonica rice introgression lines carrying Bph1 or bph2 resistance genes.

    Naeemullah N, MATSUMURA M, MORI Naoki, TAKEDA Makio, NAKAMURA Chiharu

    5th Int. Rice Genet. Symp. and 3rd Int. Rice Funct. Genomics Symp.,, 2005年11月, 英語, 未記入, Manila, Philippines, 国際会議

    口頭発表(一般)

  • コムギ近縁野生種Aegilops neglectaとAe. columnarisのトルコ南部における地理的分布

    大田 正次, HAKAN Ozkan, 森 直樹

    日本育種学会第107 ・108 回講演会, 2005年08月, 日本語, 日本育種学会, 筑波大学, 国内会議

    口頭発表(一般)

  • コムギにおけるMutator様転移酵素配列の同定とイネ科植物内での分布

    朝倉 史明, 吉田 晋弥, 森 直樹, 大塚 一郎, 中村 千春

    日本育種学会第107 ・108 回講演会, 2005年08月, 日本語, 日本育種学会, 筑波大学, 国内会議

    口頭発表(一般)

  • コムギ-エギロプス属2倍体種のPolA1 遺伝子第19イントロンのDNA 多型

    高橋 弘子, 森 直樹, 河原 太八, 中村 郁郎

    日本育種学会第107 ・108 回講演会, 2005年08月, 日本語, 日本育種学会, 筑波大学, 国内会議

    口頭発表(一般)

  • 普通糸コムギならびにその祖先種でみられたコムギミトコンドリアマイクロサテライト座における多様性

    高橋 知佐, 森 直樹, 上島 脩志, 石井 尊生

    近畿作物・育種研究会第159回例会, 2005年, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 葉緑体DNAのマイクロサテライト多型からみたエンマーコムギと裸性二粒系コムギの遺伝的分化

    森 直樹, 田中 知秋, 秀平 裕子, 綿谷 浩之, 河原 太八, 中村 千春

    日本遺伝学会第76回講演会, 2004年09月, 日本語, 日本遺伝学会, 大阪大学, 国内会議

    口頭発表(一般)

  • 二粒系コムギの栽培化と普通系コムギの進化

    森 直樹

    日本育種学会第106回講演会, 2004年09月, 日本語, 日本育種学会, 三重大学, 国内会議

    口頭発表(一般)

  • 現存するコムギのDNAからみた栽培コムギの起原

    森 直樹

    地球環境研究所シンポジウム, 2004年09月, 日本語, 未記入, 国際日本文化研究センター, 国内会議

    口頭発表(一般)

  • イネ幼病期低温耐性のQTL解析

    Aloka Lanka Ranawake, 森 直樹, 青木 法明, 梅本 貴之, 吉田 晋也, 中村 千春

    日本育種学会第106回講演会, 2004年09月, 日本語, 日本育種学会, 三重大学, 国内会議

    口頭発表(一般)

  • 現世コムギのDNAからみた栽培コムギの起原

    森 直樹

    地球環境研究所シンポジウム, 2004年07月, 日本語, 未記入, 国際日本文化研究センター, 国内会議

    口頭発表(一般)

  • 祖先2倍種の葉緑体DNA における分子多型から見た4倍性コムギの起原

    綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹

    日本育種学会 育種学研究, 2003年09月, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • AMF とSAGE による酒米の胚乳発達過程における遺伝子発現の解析

    川村 雅志, 寺内 良平, 松村 英生, 吉田 晋弥, 中村 千春, 森 直樹

    日本育種学会 育種学研究, 2003年09月, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • ペレニアルライグラスいもち病菌の地理的機嫌の解明とコムギに対する発病ポテンシャルの評価

    中川 志織, 土佐 幸雄, 平田 健治, 中馬 いづみ, 磯部 千尋, 中屋敷 均, 森 直樹, WAKAR Uddin, 眞山 滋志

    平成15年日本植物病理学会大会,日本植物病理学会報, 2003年04月, 日本語, 日本植物病理学会, 明治大学, 国内会議

    口頭発表(一般)

  • 祖先2倍種の葉緑体DNA における分子多型からみた4倍性コムギの起原

    綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • Origin of domesticated emmer and common wheat inferred from chloriplast DNA finger printing.

    MORI Naoki, ISHII T, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA S, NAKAMURA Chiharu

    Ⅹth Int. Wheat Genet. Symp., 2003年, 英語, 未記入, Paestum, Italy., 国際会議

    口頭発表(一般)

  • Mitochondrial genome and genes of common wheat, Triticum aestivum cv. Chinese Spring.

    OGIHARA Y, FUTO S, KANNO A, MIYASHITA N, NASUDA S, SHIINA T, TERACHI T, GUO C-H, NAKAMURA Chiharu, MORI Naoki, TAKUMI Shigeo, MURATA M, YAMAZAKI Y, MURAI K, MATSUOKA Y, TSUNEWAKI K

    Ⅹth Int. Wheat Genet. Symp., 2003年, 英語, 未記入, Paestum, Italy., 国際会議

    口頭発表(一般)

所属学協会

  • 日本進化学会

    2020年 - 現在
  • 米国遺伝学会

  • 日本育種学会

  • 日本遺伝学会

共同研究・競争的資金等の研究課題