研究者紹介システム

大森 崇
オオモリ タカシ
医学部附属病院 臨床研究推進センター
教授
経営工学関係
Last Updated :2020/06/04

研究者情報

所属

  • 【主配置】

    医学部附属病院 臨床研究推進センター
  • 【配置】

    医学部 医学科, 大学院医学研究科 バイオメディカルサイエンス専攻, 大学院医学研究科 医科学専攻, 数理・データサイエンスセンター

学位

  • 博士(工学), 東京理科大学

授業科目

  • バイオサイエンス基本実習, 大学院医学研究科 バイオメディカルサイエンス専攻, 2019
  • バイオメディカルサイエンス特別研究, 大学院医学研究科 バイオメディカルサイエンス専攻, 2019
  • 統計学, 大学院医学研究科 バイオメディカルサイエンス専攻, 2019
  • 文献解析・プレゼンテーション演習, 大学院医学研究科 バイオメディカルサイエンス専攻, 2019
  • 生命科学論文・申請書作成特論, 大学院医学研究科 医科学専攻, 2019
  • 情報科学, 医学部 医学科, 2019
  • 臨床研究システム論, 医学部 医学科, 2019

研究活動

受賞

  • 2016年11月 一般財団法人日本化学工業協会 日化協LRI賞 コメットアッセイJaCVAM国際バリデーション研究における中央値に基づく再解析の結果

    大森崇

    出版社・新聞社・財団等の賞

論文

  • Development of a modified 3T3 Neutral Red Uptake Phototoxicity Test protocol for evaluation of poorly water-soluble substances

    Akemi Toyoda, Maki Sugiyama, Seiichiro Furihata, Keiji Nishizumi, Takashi Omori, Hiroshi Itagaki

    The 3T3 neutral red uptake phototoxicity test (OECD TG432) is an alternative photo toxicity test method that is relatively easy and rapid to implement, with results obtainable in a short time, and is reported to have high reproducibility compared with in vivo assay methods. However, this method has been shown to be unsuitable for testing poorly water-soluble substances, which tend to separate out when mixed with the assay buffer solution. This causes difficulties in determining the dose dependency of substances and subsequent determination of the photoirritation factor because the ratio of cell viability, expressed as the half-maximal inhibitory concentration (IC50) in the presence or absence of light, is not calculable. In this study, we investigated the optimum conditions for the evaluation of poorly water-soluble substances. In the conventional method, the final solvent concentration was 1% and the pre-incubation time was 60 min, but in the modified method, 10% and 5 min were used, respectively. Next, the results from the conventional method were compared with those of our modified method, which was found to be viable and comparable with the conventional method. Moreover, the false positive results frequently obtained with poorly water-soluble substances in the conventional method were not evident with the modified method, thus confirming its usefulness for the evaluation of such substances. We therefore propose that the modified method can be used for the in vitro testing of poorly water-soluble substances in photo toxicity evaluations.

    JAPANESE SOC TOXICOLOGICAL SCIENCES, 2017年10月, JOURNAL OF TOXICOLOGICAL SCIENCES, 42 (5), 569 - 577, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hirohisa Nagahori, Noriyuki Suzuki, Florian Le Coz, Takashi Omori, Koichi Saito

    Hand1-Luc Embryonic Stem Cell Test (Hand1-Luc EST) is a promising alternative method for evaluation of developmental toxicity. However, the problems of predictivity have remained due to appropriateness of the solubility, metabolic system, and prediction model. Therefore, we assessed the usefulness of rat liver S9 metabolic stability test using LC-MS/MS to develop new prediction model. A total of 71 chemicals were analyzed by measuring cytotoxicity and differentiation toxicity, and highly reproducible (CV = 20%) results were obtained. The first prediction model was developed by discriminant analysis performed on a full dataset using Hand1-Luc EST, and 66.2% of the chemicals were correctly classified by the cross validated classification. A second model was developed with additional descriptors obtained from the metabolic stability test to calculate hepatic availability, and an accuracy of 83.3% was obtained with applicability domain of 50.7% (=36/71) after exclusion of 22 metabolically inapplicable candidates, which potentially have a metabolic activation property. A step-wise prediction scheme with combination of Hand1-Luc EST and metabolic stability test was therefore proposed. The current results provide a promising in vitro test method for accurately predicting in vivo developmental toxicity. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

    ELSEVIER IRELAND LTD, 2016年09月, TOXICOLOGY LETTERS, 259, 44 - 51, 英語

    [査読有り]

    研究論文(学術雑誌)

  • UnoY, OmoriT

    2015年07月, Mutat Res Genet Toxicol Environ Mutagen, 786788, 182 - 7, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yoshifumi Uno, Hajime Kojima, Takashi Omori, Raffaella Corvi, Masamistu Honma, Leonard M. Schechtman, Raymond R. Tice, Brian Burlinson, Patricia A. Escobar, Andrew R. Kraynak, Yuzuki Nakagawa, Madoka Nakajima, Kamala Pant, Norihide Asano, David Lovell, Takeshi Morita, Yasuo Ohnob, Makoto Hayashi

    The in vivo rodent alkaline comet assay (comet assay) is used internationally to investigate the in vivo genotoxic potential of test chemicals. This assay, however, has not previously been formally validated. The Japanese Center for the Validation of Alternative Methods (JaCVAM), with the cooperation of the U.S. NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM)/the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Centre for the Validation of Alternative Methods (ECVAM), and the Japanese Environmental Mutagen Society/Mammalian Mutagenesis Study Group (JEMS/MMS), organized an international validation study to evaluate the reliability and relevance of the assay for identifying genotoxic carcinogens, using fiver and stomach as target organs. The ultimate goal of this validation effort was to establish an Organisation for Economic Co-operation and Development (OECD) test guideline. The purpose of the pre-validation studies (i.e., Phase 1 through 3), conducted in four or five laboratories with extensive comet assay experience, was to optimize the protocol to be used during the definitive validation study. (C) 2015 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2015年07月, MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 786, 3 - 13, 英語

    [査読有り]

    研究論文(学術雑誌)

  • UnoY, KojimaH, OmoriT, CorviR, HonmaM, SchechtmanLM, TiceRR, BeeversC, DeBoeckM, BurlinsonB, HobbsCA, KitamotoS, KraynakAR, McNameeJ, NakagawaY, PantK, Plappert-HelbigU, PriestleyC, TakasawaH, WadaK, WirnitzerU, AsanoN, EscobarPA

    2015年07月, Mutat Res Genet Toxicol Environ Mutagen, 786788, 45 - 76, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • 統計教育に関する授業内容の傾向と学生の授業評価-統計教育質保証に関する分析調査-.

    大森崇

    2013年, 9 (1), 1 - 11

  • Masao Suzuki, Shigeo Muro, Yuki Ando, Takashi Omori, Tetsuhiro Shiota, Kazuo Endo, Susumu Sato, Kensaku Aihara, Masataka Matsumoto, Shinko Suzuki, Ryo Itotani, Manabu Ishitoko, Yoshikazu Hara, Masaya Takemura, Tetsuya Ueda, Hitoshi Kagioka, Masataka Hirabayashi, Motonari Fukui, Michiaki Mishima

    Background: Dyspnea on exertion (DOE) is a major symptom of chronic obstructive pulmonary disease (COPD) and is difficult to control. This study was performed to determine whether acupuncture is superior to placebo needling in improving DOE in patients with COPD who are receiving standard medication. Methods: Sixty-eight of 111 patients from the Kansai region of Japan who were diagnosed as having COPD and were receiving standard medication participated in a randomized, parallel-group, placebo-controlled trial (July 1, 2006, through March 31, 2009) in which the patients, evaluators, and statistician were unaware of the random allocation. Participants were randomly assigned to traditional acupuncture (real acupuncture group, n = 34) or placebo needling (placebo acupuncture group, n = 34). Both groups received real or placebo needling at the same acu-points once a week for 12 weeks. The primary end point was the modified Borg scale score evaluated immediately after the 6-minute walk test. Measurements were obtained at baseline and after 12 weeks of treatment. Result: After 12 weeks, the Borg scale score after the 6-minute walk test was significantly better in the real acupuncture group compared with the placebo acupuncture group (mean [SD] difference from baseline by analysis of covariance, -3.6 [1.9] vs 0.4 [1.2]; mean difference between groups by analysis of covariance, -3.58; 95% CI, -4.27 to -2.90). Patients with COPD who received real acupuncture also experienced improvement in the 6-minute walk distance during exercise, indicating better exercise tolerance and reduced DOE. Conclusion: This study clearly demonstrates that acupuncture is a useful adjunctive therapy in reducing DOE in patients with COPD.

    AMER MEDICAL ASSOC, 2012年06月, ARCHIVES OF INTERNAL MEDICINE, 172 (11), 878 - 886, 英語

  • Validation study of in vitro skin irritation test with the LabCyte EPI-MODEL24.

    大森 崇

    2012年, ATLA, (40), 33–50

  • Validation Study of the in vitro skin irritation test with the LabCyte EPI-MODEL24.

    Omori, T

    2012年, ATAL, 40, 33 - 50

  • Hitoshi Sakaguchi, Naoko Ota, Takashi Omori, Hirofumi Kuwahara, Takashi Sozu, Yumi Takagi, Yutaka Takahashi, Kouko Tanigawa, Miki Nakanishi, Tsuneaki Nakamura, Takashi Morimoto, Shinobu Wakuri, Yuko Okamoto, Mayumi Sakaguchi, Takumi Hayashi, Takayuki Hanji, Shinichi Watanabe

    Short Time Exposure (STE) test is a cytotoxicity test in SIRC cells (rabbit corneal cell line) that assesses eye irritation potential following a 5-min chemical exposure. This validation study assessed transferability, intra- and inter-laboratory reproducibility, and predictive capacity of STE test in five laboratories (supported by Japanese Society for Alternatives to Animal Experiments). Sodium lauryl sulfate, calcium thioglycolate, and Tween 80 were evaluated, in triplicate, using 5%, 0.5%, and 0.05% concentrations in physiological saline, to confirm transferability. Good transferability was noted when similar mean relative viabilities and rank classifications were obtained in all five laboratories and were comparable to data from test method developing laboratory. Good intra- and inter-laboratory reproducibility was obtained with four assay controls (three solvents and one positive control), and four assay controls and 25 chemicals, respectively. STE irritation category based on relative viability of a 5% solution of 25 blinded test chemicals showed good correlation with Globally Harmonized System (GHS) categories (NI; I: Cat. 1 and 2). The STE prediction model, using relative viability of the 5% and 0.05% solutions, provided an irritation rank (1, 2, or 3) that had a good correlation (above 80%), or predictive capacity, with GHS irritation ranks in all laboratories. Based on these findings, the STE test is a promising alternative eye irritation test that could be easily standardized. (C) 2011 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2011年06月, TOXICOLOGY IN VITRO, 25 (4), 796 - 809, 英語

  • Hitoshi Sakaguchi, Naoko Ota, Takashi Omori, Hirofumi Kuwahara, Takashi Sozu, Yumi Takagi, Yutaka Takahashi, Kouko Tanigawa, Miki Nakanishi, Tsuneaki Nakamura, Takashi Morimoto, Shinobu Wakuri, Yuko Okamoto, Mayumi Sakaguchi, Takumi Hayashi, Takayuki Hanji, Shinichi Watanabe

    Short Time Exposure (STE) test is a cytotoxicity test in SIRC cells (rabbit corneal cell line) that assesses eye irritation potential following a 5-min chemical exposure. This validation study assessed transferability, intra- and inter-laboratory reproducibility, and predictive capacity of STE test in five laboratories (supported by Japanese Society for Alternatives to Animal Experiments). Sodium lauryl sulfate, calcium thioglycolate, and Tween 80 were evaluated, in triplicate, using 5%, 0.5%, and 0.05% concentrations in physiological saline, to confirm transferability. Good transferability was noted when similar mean relative viabilities and rank classifications were obtained in all five laboratories and were comparable to data from test method developing laboratory. Good intra- and inter-laboratory reproducibility was obtained with four assay controls (three solvents and one positive control), and four assay controls and 25 chemicals, respectively. STE irritation category based on relative viability of a 5% solution of 25 blinded test chemicals showed good correlation with Globally Harmonized System (GHS) categories (NI; I: Cat. 1 and 2). The STE prediction model, using relative viability of the 5% and 0.05% solutions, provided an irritation rank (1, 2, or 3) that had a good correlation (above 80%), or predictive capacity, with GHS irritation ranks in all laboratories. Based on these findings, the STE test is a promising alternative eye irritation test that could be easily standardized. (C) 2011 Elsevier Ltd. All rights reserved.

    PERGAMON-ELSEVIER SCIENCE LTD, 2011年06月, TOXICOLOGY IN VITRO, 25 (4), 796 - 809, 英語

  • Hajime Kojima, Masahiro Takeyoshi, Takashi Sozu, Takumi Awogi, Kazunori Arima, Kenji Idehara, Yoshiaki Ikarashi, Yukiko Kanazawa, Eiji Maki, Takashi Omori, Atsuko Yuasa, Isao Yoshimura

    The murine local lymph node assay (LLNA) is a well-established alternative to the guinea pig maximization test (GPMT) or Buehler test (BT) for the assessment of the skin sensitizing ability of a drug, cosmetic material, pesticide or industrial chemical. Instead of radioisotope using in this method, Takeyoshi M. et al. (2001) has developed a modified LLNA based on the 5-bromo-2'-deoxyuridine (BrdU) incorporation (LLNA:BrdU-ELISA). The LLNA:BrdU-ELISA is practically identical to the LLNA methodology excluding the use of BrdU, for which a single intraperitoneal injection of BrdU is made on day 4, and colorimetric detection of cell turnover. We conducted the validation study to evaluate the reliability and relevance of LLNA:BrdU-ELISA. The experiment involved 7 laboratories, wherein 10 chemicals were examined under blinded conditions. In this study, 3 chemicals were examined in all laboratories and the remaining 7 were examined in 3 laboratories. The data were expressed as the BrdU incorporation using an ELISA method for each group, and the stimulation index (SI) for each chemical-treated group was determined as the increase in the BrdU incorporation relative to the concurrent vehicle control group. An SI of 2 was set as the cut-off value for exhibiting skin sensitization activity. The results obtained in the experiments conducted for all 10 chemicals were sufficiently consistent with small variations in their SI values. The sensitivity, specificity, and accuracy of LLNA:BrdU-ELISA against those of GPMT/BT were 7/7 (100%), 3/3 (100%), and 10/10 (100%), respectively. Copyright (C) 2010 John Wiley & Sons, Ltd.

    JOHN WILEY & SONS LTD, 2011年01月, JOURNAL OF APPLIED TOXICOLOGY, 31 (1), 63 - 74, 英語

  • Inter-laboratory validation of the modified murine local lymph node assay based on 5-bromo-2’-deoxyuridine (BrdU) incorporation.

    大森 崇

    2011年, Journal of Applied Toxicology, 31, 63–74

  • Hajime Kojima, Masahiro Takeyoshi, Takashi Sozu, Takumi Awogi, Kazunori Arima, Kenji Idehara, Yoshiaki Ikarashi, Yukiko Kanazawa, Eiji Maki, Takashi Omori, Atsuko Yuasa, Isao Yoshimura

    The murine local lymph node assay (LLNA) is a well-established alternative to the guinea pig maximization test (GPMT) or Buehler test (BT) for the assessment of the skin sensitizing ability of a drug, cosmetic material, pesticide or industrial chemical. Instead of radioisotope using in this method, Takeyoshi M. et al. (2001) has developed a modified LLNA based on the 5-bromo-2'-deoxyuridine (BrdU) incorporation (LLNA:BrdU-ELISA). The LLNA:BrdU-ELISA is practically identical to the LLNA methodology excluding the use of BrdU, for which a single intraperitoneal injection of BrdU is made on day 4, and colorimetric detection of cell turnover. We conducted the validation study to evaluate the reliability and relevance of LLNA:BrdU-ELISA. The experiment involved 7 laboratories, wherein 10 chemicals were examined under blinded conditions. In this study, 3 chemicals were examined in all laboratories and the remaining 7 were examined in 3 laboratories. The data were expressed as the BrdU incorporation using an ELISA method for each group, and the stimulation index (SI) for each chemical-treated group was determined as the increase in the BrdU incorporation relative to the concurrent vehicle control group. An SI of 2 was set as the cut-off value for exhibiting skin sensitization activity. The results obtained in the experiments conducted for all 10 chemicals were sufficiently consistent with small variations in their SI values. The sensitivity, specificity, and accuracy of LLNA:BrdU-ELISA against those of GPMT/BT were 7/7 (100%), 3/3 (100%), and 10/10 (100%), respectively. Copyright (C) 2010 John Wiley & Sons, Ltd.

    JOHN WILEY & SONS LTD, 2011年01月, JOURNAL OF APPLIED TOXICOLOGY, 31 (1), 63 - 74, 英語

  • Hiroshi Nitta, Shin Yamazaki, Takashi Omori, Tosiya Sato

    Many developments in the design and analysis of environmental epidemiology have been made in air pollution studies. In the analysis of the short-term effects of particulate matter on daily mortality. Poisson regression models with flexible smoothing methods have been developed for the analysis of time-series data. Another option for such studies is the use of case crossover designs, and there have been extensive discussions on the selection of control periods. In the Study on Respiratory Disease and Automobile Exhaust project conducted by the Japanese Ministry of the Environment, we adopted a new 2-stage case control design that is efficient when both exposure and disease are rare. Based on our experience in conducting air pollution epidemiologic studies, we review 2-stage case control designs, case crossover designs, generalized linear models, generalized additive models, and generalized estimating equations, all of which are useful approaches in environmental epidemiology.

    JAPAN EPIDEMIOLOGICAL ASSOC, 2010年05月, JOURNAL OF EPIDEMIOLOGY, 20 (3), 177 - 184, 英語

    書評論文,書評,文献紹介等

  • Hiroshi Nitta, Shin Yamazaki, Takashi Omori, Tosiya Sato

    Many developments in the design and analysis of environmental epidemiology have been made in air pollution studies. In the analysis of the short-term effects of particulate matter on daily mortality. Poisson regression models with flexible smoothing methods have been developed for the analysis of time-series data. Another option for such studies is the use of case crossover designs, and there have been extensive discussions on the selection of control periods. In the Study on Respiratory Disease and Automobile Exhaust project conducted by the Japanese Ministry of the Environment, we adopted a new 2-stage case control design that is efficient when both exposure and disease are rare. Based on our experience in conducting air pollution epidemiologic studies, we review 2-stage case control designs, case crossover designs, generalized linear models, generalized additive models, and generalized estimating equations, all of which are useful approaches in environmental epidemiology.

    JAPAN EPIDEMIOLOGICAL ASSOC, 2010年05月, JOURNAL OF EPIDEMIOLOGY, 20 (3), 177 - 184, 英語

    書評論文,書評,文献紹介等

  • Hisashi Noma, Shigeyuki Matsui, Takashi Omori, Tosiya Sato

    The main purpose of microarray studies is screening to identify differentially expressed genes as candidates for further investigation. Because of limited resources in this stage, prioritizing or ranking genes is a relevant statistical task in microarray studies. In this article, we develop 3 empirical Bayes methods for gene ranking on the basis of differential expression, using hierarchical mixture models. These methods are based on (i) minimizing mean squared errors of estimation for parameters, (ii) minimizing mean squared errors of estimation for ranks of parameters, and (iii) maximizing sensitivity in selecting prespecified numbers of differential genes, with the largest effect. Our methods incorporate the mixture structures of differential and nondifferential components in empirical Bayes models to allow information borrowing across differential genes, with separation from nuisance, nondifferential genes. The accuracy of our ranking methods is compared with that of conventional methods through simulation studies. An application to a clinical study for breast cancer is provided.

    OXFORD UNIV PRESS, 2010年04月, BIOSTATISTICS, 11 (2), 281 - 289, 英語

  • Validation studies on an alternative endpoint for the local lymph node assay (LLNA-DA): Importance of study management

    2008年, Alternative to Animal Testing and Experimentation, 14 (Special issue), 429 - 432

  • 大森 崇

    2008年, Mutagenesis, 23 (3), 171 - 182

  • 大森 崇

    2008年, Journal of Pharmacological and Toxicological Methods, 58 (1), 11 - 26

  • An evaluation of performance standards and non-radioactive endpoints for the local lymph node assay

    大森 崇

    2008年, ATLA, 36, 243 - 257

  • 重篤な有害事象を速く検出するためのシグナル検出法の検討

    大森 崇

    2007年, 日本統計学会誌, (2), 195 - 204

  • 大森 崇

    2007年, Alternative to Animal Testing and Experimentation, 12 (3), 212 - 217

  • An evaluation of performance standards and non-radioactive endpoints for the local lymph node assay.

    Omori, T

    ATLA, 36, 243 - 257

書籍等出版物

  • R Commanderによるデータ解析

    共立出版, 2010年

  • 医学・薬学・健康の統計学

    サイエンティスト社, 2009年

講演・口頭発表等

  • 診療科モニタリングを支える補助ツールとしてのファイリングシステムの導入

    筧 康正, 小谷 保之, 鳥越 奈穂, 石田 裕紀, 山崎 純子, 柏木 直子, 五百蔵 武士, 久米 学, 大森 崇, 永井 洋士

    日本臨床試験学会第9回学術集会総会, 2018年02月, 日本語, 日本臨床試験学会, 仙台, 国内会議

    口頭発表(一般)

  • Topical cutaneous application of carbon dioxide using a hydrogel is a new treatment option to accelerate fracture repair -Results of a clinical trial-

    Takahiro Niikura, Takashi Iwakura, Takashi Omori, Sang Yang Lee, Tomoaki Fukui, Keisuke Oe, Yoshitada Sakai, Toshihiro Akisue, Kotaro Nishida, Ryosuke Kuroda

    Asia Pacific Orthopaedic Association Trauma & Infection Meeting 2017, 2017年04月, 英語, Asia Pacific Orthopaedic Association, Putrajaya, Malaysia, We have reported that topical cutaneous application of CO2 using a hydrogel accelerates fracture repair by increasing blood flow, angiogenesis and promoting endochondral ossification in rats [1]. Now we apply this novel therapy to human subjects, and assess the safety and efficacy., 国際会議

    口頭発表(一般)

共同研究・競争的資金等の研究課題

  • (AMED)睡眠脳波を用いたうつ病の客観的評価方法の実用化に関する研究

    大森 崇

    国立研究開発法人日本医療研究開発機構, 医療機器開発推進研究事業, 2017年, 研究代表者

    競争的資金

  • (AMED)睡眠脳波を用いたうつ病の客観的評価方法の実用化に関する研究

    大森 崇

    国立研究開発法人日本医療研究開発機構, 医療機器開発推進研究事業, 2016年, 研究代表者

    競争的資金