研究者紹介システム

三宅 親弘
ミヤケ チカヒロ
大学院農学研究科 生命機能科学専攻
教授
農芸化学関係
Last Updated :2021/04/12

研究者情報

所属

  • 【主配置】

    大学院農学研究科 生命機能科学専攻
  • 【配置】

    農学部 生命機能科学科

学位

  • 博士(農学), 京都大学

授業科目

ミニ講義

ジャンル

  • 科学・技術 / 生物

コメントテーマ

  • 光合成
  • 活性酸素
  • 植物が枯れるメカニズム

研究ニュース

研究活動

研究分野

  • ライフサイエンス / 植物栄養学、土壌学

論文

  • Yoshifumi Ueno, Ginga Shimakawa, Shimpei Aikawa, Chikahiro Miyake, Seiji Akimoto

    Oxygenic photosynthesis converts light energy into chemical energy via electron transport and assimilates CO2 in the Calvin-Benson cycle with the chemical energy. Thus, high light and low CO2 conditions induce the accumulation of electrons in the photosynthetic electron transport system, resulting in the formation of reactive oxygen species. To prevent the accumulation of electrons, oxygenic photosynthetic organisms have developed photoprotection mechanisms, including non-photochemical quenching (NPQ) and alternative electron flow (AEF). There are diverse molecular mechanisms underlying NPQ and AEF, and the corresponding molecular actors have been identified and characterized using a model green alga Chlamydomonas reinhardtii. In contrast, detailed information about the photoprotection mechanisms is lacking for other green algal species. In the current study, we examined the photoprotection mechanisms responsive to CO2 in the green alga Chlorella variabilis by combining the analyses of pulse-amplitude-modulated fluorescence, O2 evolution, and the steady-state and time-resolved fluorescence spectra. Under the CO2-limited condition, ΔpH-dependent NPQ occurred in photosystems I and II. Moreover, O2-dependent AEF was also induced. Under the CO2-limited condition with carbon supplementation, NPQ was relaxed and light-harvesting chlorophyll-protein complex II was isolated from both photosystems. In C. variabilis, the O2-dependent AEF and the mechanisms that instantly convert the light-harvesting functions of both photosystems may be important for maintaining efficient photosynthetic activities under various CO2 conditions.

    2020年06月, Photosynthesis research, 144 (3), 397 - 407, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Growth light environment changes the sensitivity of photosystem I photoinhibition by depending on common wheat cultivars

    Daisuke Takagi, HIroaki Ihara, Shigeo Takumi, Chikahiro Miyake

    2019年06月, Frontiers in Plant Science, 10, 686, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 和田 慎也, 高木 大輔, 三宅 親弘, 牧野 周, 鈴木 雄二

    MDPI, 2019年04月, International Journal of Molecular Sciences, 20 (9), 2068, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Comparative analysis of strategies to prepare electron sinks in aquatic photoautotrophs

    Shimakawa Ginga, 村上明男, Niwa Kyosuke, Matsuda Yusuke, Wada Ayumi, MIyake Chikahiro

    2019年03月, Photosynthesis Research, 139 (1-3), 401 - 411, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga SHIMAKAWA, Kentaro IFUKU, Yuji SUZUKI, Amane MAKINO, Kimitsune ISHIZAKI, Hiroshi FUKAYAMA, Ryutaro MORITA, Katsuhiko SAKAMOTO, Akiko NISHI, Chikahiro MIYAKE

    Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2018年12月, Bioscience, Biotechnology and Biochemistry, 82 (12), 2072 - 2083, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga Shimakawa, Keiichiro Shaku, Chikahiro Miyake

    Photosynthetic organisms oxidize P700 to suppress the production of reactive oxygen species (ROS) in photosystem I (PSI) in response to the lower efficiency of photosynthesis under high light and low CO2 conditions. Previously, we found a positive relationship between reduction of plastoquinone (PQ) pool and oxidation of P700, which we named reduction-induced suppression of electron flow (RISE). In the RISE model, we proposed that the highly reduced state of the PQ pool suppresses Q-cycle turnover to oxidize P700 in PSI. Here, we tested whether RISE was relieved by the oxidation of the PQ pool, but not by the dissipation of the proton gradient (ΔpH) across the thylakoid membrane. Formation of ΔpH can also suppress electron flow to P700, because acidification on the luminal side of the thylakoid membrane lowers oxidation of reduced PQ in the cytochrome b6/f complex. We drove photosynthetic electron transport using H2O2-scavenging peroxidase reactions. Peroxidase reduces H2O2 with electron donors regenerated along the photosynthetic electron transport system, thereby promoting the formation of ΔpH. Addition of H2O2 to the cyanobacterium Synechococcus elongatus PCC 7942 under low CO2 conditions induced photochemical quenching of chlorophyll fluorescence, enhanced NADPH fluorescence and reduced P700. Thus, peroxidase reactions relieved the RISE mechanism, indicating that P700 oxidation can be induced only by the reduction of PQ to suppress the production of ROS in PSI. Overall, our data suggest that RISE regulates the redox state of P700 in PSI in cooperation with ΔpH regulation.

    Frontiers Media S.A., 2018年05月07日, Frontiers in Microbiology, 9 (MAY), 英語

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    研究論文(学術雑誌)

  • Daisuke Takagi, Kentaro Ifuku, Taishi Nishimura, Chikahiro Miyake

    Springer Nature, 2018年05月, Photosynthesis Research, 139 (1-3), 487, 英語

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    研究論文(学術雑誌)

  • Ginga Shimakawa, Chikahiro Miyake

    Oxygenic phototrophs are vulnerable to damage by reactive oxygen species (ROS) that are produced in photosystem I (PSI) by excess photon energy over the demand of photosynthetic CO2 assimilation. In plant leaves, repetitive short-pulse (rSP) illumination produces ROS to inactivate PSI. The production of ROS is alleviated by oxidation of the reaction center chlorophyll in PSI, P700, during the illumination with the short-pulse light, which is supported by flavodiiron protein (FLV). In this study, we found that in the cyanobacterium Synechocystis sp. PCC 6803 P700 was oxidized and PSI was not inactivated during rSP illumination even in the absence of FLV. Conversely, the mutant deficient in respiratory terminal oxidases was impaired in P700 oxidation during the illumination with the short-pulse light to suffer from photo-oxidative damage in PSI. Interestingly, the other cyanobacterium Synechococcus sp. PCC 7002 could not oxidize P700 without FLV during rSP illumination. These data indicate that respiratory terminal oxidases are critical to protect PSI from ROS damage during rSP illumination in Synechocystis sp. PCC 6803 but not Synechococcus sp. PCC 7002.

    Springer Netherlands, 2018年03月08日, Photosynthesis Research, 1 - 10, 英語

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    研究論文(学術雑誌)

  • Effects of genetic manipulation of the activity of photorespiration on the redox state of photosystem I and its robustness against excess light stress under CO₂-limited conditions in rice

    Shinya Wada, Yuji Suzuki, Daisuke Takagi, Chikahiro Miyake, Amane Makino

    2018年, Photosynthesis Research, 英語

    [査読有り]

    研究論文(学術雑誌)

  • PROTON GRADIENT REGULATION 5 supports linear electron flow to oxidize photosystem I

    Daisuke Takagi, Chikahiro Miyake

    2018年, Physiologia Plantaruim, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Light-harvesting strategy during CO2-dependent photosynthesis in the green alga Chlamydomonas reinhardtii

    Y. Ueno, G. Shimakawa, C. Miyake, S. Akimoto

    2018年, J. Phys. Chem. Lett., 9, 1028 - 1033, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga Shimakawa, Satoru Watanabe, Chikahiro Miyake

    A limitation in carbon dioxide (CO2), which occurs as a result of natural environmental variation, suppresses photosynthesis and has the potential to cause photo-oxidative damage to photosynthetic cells. Oxygenic phototrophs have strategies to alleviate photo-oxidative damage to allow life in present atmospheric CO2 conditions. However, the mechanisms for CO2 limitation acclimation are diverse among the various oxygenic phototrophs, and many mechanisms remain to be discovered. In this study, we found that the gene encoding a CO2 limitation-inducible protein, ColA, is required for the cyanobacterium Synechococcus sp. PCC 7002 (S. 7002) to acclimate to limited CO2 conditions. An S. 7002 mutant deficient in ColA (Delta colA) showed lower chlorophyll content, based on the amount of nitrogen, than that in S. 7002 wild-type (WT) under ambient air but not high CO2 conditions. Both thermoluminescence and protein carbonylation detected in the ambient air grown cells indicated that the lack of ColA promotes oxidative stress in S. 7002. Alterations in the photosynthetic O-2 evolution rate and relative electron transport rate in the short-term response, within an hour, to CO2 limitation were the same between the WT and Delta colA. Conversely, these photosynthetic parameters were mostly lower in the long-term response of a few days in Delta colA than in the WT. These data suggest that ColA is required to sustain photosynthetic activity for living under ambient air in S. 7002. The unique phylogeny of ColA revealed diverse strategies to acclimate to CO2 limitation among cyanobacteria.

    MDPI AG, 2017年12月, MARINE DRUGS, 15 (12), 英語

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    研究論文(学術雑誌)

  • Daisuke Takagi, Kimitsune Ishizaki, Hitomi Hanawa, Tomohito Mabuchi, Ginga Shimakawa, Hiroshi Yamamoto, Chikahiro Miyake

    In land plants, photosystem I (PSI) photoinhibition limits carbon fixation and causes growth defects. In addition, recovery from PSI photoinhibition takes much longer than PSII photoinhibition when the PSI core-complex is degraded by oxidative damage. Accordingly, PSI photoinhibition should be avoided in land plants, and land plants should have evolved mechanisms to prevent PSI photoinhibition. However, such protection mechanisms have not yet been identified, and it remains unclear whether all land plants suffer from PSI photoinhibition in the same way. In the present study, we focused on the susceptibility of PSI to photoinhibition and investigated whether mechanisms of preventing PSI photoinhibition varied among land plant species. To assess the susceptibility of PSI to photoinhibition, we used repetitive short-pulse (rSP) illumination, which specifically induces PSI photoinhibition. Subsequently, we found that land plants possess a wide variety of tolerance mechanisms against PSI photoinhibition. In particular, gymnosperms, ferns and mosses/liverworts exhibited higher tolerance to rSP illumination-induced PSI photoinhibition than angiosperms, and detailed analyses indicated that the tolerance of these groups could be partly attributed to flavodiiron proteins, which protected PSI from photoinhibition by oxidizing the PSI reaction center chlorophyll (P700) as an electron acceptor. Furthermore, we demonstrate, for the first time, that gymnosperms, ferns and mosses/liverworts possess a protection mechanism against photoinhibition of PSI that differs from that of angiosperms.

    Blackwell Publishing Ltd, 2017年09月01日, Physiologia Plantarum, 161 (1), 56 - 74, 英語

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    研究論文(学術雑誌)

  • Hitomi Hanawa, Kimitsune Ishizaki, Kana Nohira, Daisuke Takagi, Ginga Shimakawa, Takehiro Sejima, Keiichiro Shaku, Amane Makino, Chikahiro Miyake

    In higher plants, the electron-sink capacity of photorespiration contributes to alleviation of photoinhibition by dissipating excess energy under conditions when photosynthesis is limited. We addressed the question at which point in the evolution of photosynthetic organisms photorespiration began to function as electron sink and replaced the flavodiiron proteins which catalyze the reduction of O-2 at photosystem I in cyanobacteria. Algae do not have a higher activity of photorespiration when CO2 assimilation is limited, and it can therefore not act as an electron sink. Using land plants (liverworts, ferns, gymnosperms, and angiosperms) we compared photorespiration activity and estimated the electron flux driven by photorespiration to evaluate its electron-sink capacity at CO2-compensation point. In vivo photorespiration activity was estimated by the simultaneous measurement of O-2-exchange rate and chlorophyll fluorescence yield. All C3-plants leaves showed transient O-2-uptake after actinic light illumination (post-illumination transient O-2-uptake), which reflects photorespiration activity. Post- illumination transient O-2-uptake rates increased in the order from liverworts to angiosperms through ferns and gymnosperms. Furthermore, photorespiration-dependent electron flux in photosynthetic linear electron flow was estimated from post-illumination transient O-2-uptake rate and compared with the electron flux in photosynthetic linear electron flow in order to evaluate the electron-sink capacity of photorespiration. The electron-sink capacity at the CO2-compensation point also increased in the above order. In gymnosperms photorespiration was determined to be the main electron-sink. C3-C4 intermediate species of Flaveria plants showed photorespiration activity, which intermediate between that of C3- and C4-flaveria species. These results indicate that in the first land plants, liverworts, photorespiration started to function as electron sink. According to our hypothesis, the dramatic increase in partial pressure of O-2 in the atmosphere about 0.4 billion years ago made it possible to drive photorespiration with higher activity in liverworts.

    WILEY, 2017年09月, PHYSIOLOGIA PLANTARUM, 161 (1), 138 - 149, 英語

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    研究論文(学術雑誌)

  • Daisuke Takagi, Amako Katsumi, Masaki Hashiguchi, Hidehiro Fukaki, Kimitsune Ishizaki, Tatsuaki Goh, Yoichiro Fukao, Yosuke Sano, Tetsuya Kurata, Taku Demura, Shinichiro Sawa, Chikahiro Miyake

    2017年04月, The Plant Journal, 91, 306 - 324, 英語

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    研究論文(学術雑誌)

  • Ginga Shimakawa, Kimitsune Ishizaki, Shigeyuki Tsukamoto, Moeko Tanaka, Takehiro Sejima, Chikahiro Miyake

    The diffusion efficiency of oxygen in the atmosphere, like that of CO2, is approximately 104 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha, a basal land plant, has habitats where it is exposed to both water and the atmosphere. Furthermore, like cyanobacteria, M. polymorpha has genes encoding flavodiiron proteins (FLV). In cyanobacteria, FLVs mediate oxygen-dependent alternative electron flow (AEF) to suppress the production of reactive oxygen species. Here, we investigated whether FLVs are required for the protection of photosynthesis in M. polymorpha. A mutant deficient in the FLV1 isozyme (Delta MpFlv1) sustained photooxidative damage to photosystem I (PSI) following repetitive short-saturation pulses of light. Compared with the wild type (Takaragaike-1), Delta MpFlv1 showed the same photosynthetic oxygen evolution rate but a lower electron transport rate during the induction phase of photosynthesis. Additionally, the reaction center chlorophyll in PSI, P700, was highly reduced in DMpFlv1 but not in Takaragaike-1. These results indicate that the gene product of MpFlv1 drives AEF to oxidize PSI, as in cyanobacteria. Furthermore, FLV-mediated AEF supports the production of a proton motive force to possibly induce the nonphotochemical quenching of chlorophyll fluorescence and suppress electron transport in the cytochrome b(6)/f complex. After submerging the thalli, a decrease in photosystem II operating efficiency was observed, particularly in Delta MpFlv1, which implies that species living in these sorts of habitats require FLV-mediated AEF.

    AMER SOC PLANT BIOLOGISTS, 2017年03月, PLANT PHYSIOLOGY, 173 (3), 1636 - 1647, 英語

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    研究論文(学術雑誌)

  • Ginga Shimakawa, Yusuke Matsuda, Kensuke Nakajima, Masahiro Tamoi, Shigeru Shigeoka, Chikahiro Miyake

    Photosynthesis produces chemical energy from photon energy in the photosynthetic electron transport and assimilates CO2 using the chemical energy. Thus, CO2 limitation causes an accumulation of excess energy, resulting in reactive oxygen species (ROS) which can cause oxidative damage to cells. O-2 can be used as an alternative energy sink when oxygenic phototrophs are exposed to high light. Here, we examined the responses to CO2 limitation and O-2 dependency of two secondary algae, Euglena gracilis and Phaeodactylum tricornutum. In E. gracilis, approximately half of the relative electron transport rate (ETR) of CO2-saturated photosynthesis was maintained and was uncoupled from photosynthesis under CO2 limitation. The ETR showed biphasic dependencies on O-2 at high and low O-2 concentrations. Conversely, in P. tricornutum, most relative ETR decreased in parallel with the photosynthetic O-2 evolution rate in response to CO2 limitation. Instead, non-photochemical quenching was strongly activated under CO2 limitation in P. tricornutum. The results indicate that these secondary algae adopt different strategies to acclimatize to CO2 limitation, and that both strategies differ from those utilized by cyanobacteria and green algae. We summarize the diversity of strategies for prevention of photo-oxidative damage under CO2 limitation in cyanobacterial and algal photosynthesis.

    NATURE PUBLISHING GROUP, 2017年01月, SCIENTIFIC REPORTS, 7, 41022, 英語

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    研究論文(学術雑誌)

  • Ginga Shimakawa, Keiichiro Shaku, Chikahiro Miyake

    The photoinhibition of photosystem I (PSI) is lethal to oxygenic phototrophs. Nevertheless, it is unclear how photodamage occurs or how oxygenic phototrophs prevent it. Here, we provide evidence that keeping P700 (the reaction center chlorophyll in PSI) oxidized protects PSI. Previous studies have suggested that PSI photoinhibition does not occur in the two model cyanobacteria, Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942, when photosynthetic CO2 fixation was suppressed under low CO2 partial pressure even in mutants deficient in flavodiiron protein (FLV), which mediates alternative electron flow. The lack of FLV in Synechococcus sp. PCC 7002 (S. 7002), however, is linked directly to reduced growth and PSI photodamage under CO2-limiting conditions. Unlike Synechocystis sp. PCC 6803 and S. elongatus PCC 7942, S. 7002 reduced P700 during CO2-limited illumination in the absence of FLV, resulting in decreases in both PSI and photosynthetic activities. Even at normal air CO2 concentration, the growth of S. 7002 mutant was retarded relative to that of the wild type. Therefore, P700 oxidation is essential for protecting PSI against photoinhibition. Here, we present various strategies to alleviate PSI photoinhibition in cyanobacteria.

    AMER SOC PLANT BIOLOGISTS, 2016年11月, PLANT PHYSIOLOGY, 172 (3), 1443 - 1450, 英語

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    研究論文(学術雑誌)

  • Daisuke Takagi, Masaki Hashiguchi, Takehiro Sejima, Amane Makino, Chikahiro Miyake

    To elucidate the molecular mechanism to oxidize the reaction center chlorophyll, P700, in PSI, we researched the effects of partial pressure of O-2 (pO(2)) on photosynthetic characteristic parameters in sunflower (Helianthus annuus L.) leaves. Under low CO2 conditions, the oxidation of P700 was stimulated; however the decrease in pO(2) suppressed its oxidation. Electron fluxes in PSII [Y(II)] and PSI [Y(I)] showed pO(2)-dependence at low CO2 conditions. H+-consumption rate, estimated from Y(II) and CO2-fixation/photorespiration rates (JgH(+)), showed the positive curvature relationship with the dissipation rate of electrochromic shift signal (V (H) (+) ), which indicates H+-efflux rate from lumen to stroma in chloroplasts. Therefore, these electron fluxes contained, besides CO2-fixation/photorespiration-dependent electron fluxes, non-H+-consumption electron fluxes including Mehler-ascorbate peroxidase (MAP)-pathway. Y(I) that was larger than Y(II) surely implies the functioning of cyclic electron flow (CEF). Both MAP-pathway and CEF were suppressed at lower pO(2), with plastoquinone-pool reduced. That is, photorespiration prepares the redox-poise of photosynthetic electron transport system for CEF activity as an electron sink. Excess Y(II), [Delta Y(II)] giving the curvature relationship with V (H) (+) , and excess Y(I) [Delta CEF] giving the difference between Y(I) and Y(II) were used as an indicator of MAP-pathway and CEF activity, respectively. Although Delta Y(II) was negligible and did not show positive relationship to the oxidation-state of P700, Delta CEF showed positive linear relationship to the oxidation-state of P700. These facts indicate that CEF cooperatively with photorespiration regulates the redox-state of P700 to suppress the over-reduction in PSI under environmental stress conditions.

    SPRINGER, 2016年09月, PHOTOSYNTHESIS RESEARCH, 129 (3), 279 - 290, 英語

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    研究論文(学術雑誌)

  • Keiichiro Shaku, Ginga Shimakawa, Masaki Hashiguchi, Chikahiro Miyake

    Accumulation of electrons under conditions of environmental stress produces a reduced state in the photosynthetic electron transport (PET) system and causes the reduction of O-2 by PSI in the thylakoid membranes to produce the reactive oxygen species superoxide radical, which irreversibly inactivates PSI. This study aims to elucidate the molecular mechanism for the oxidation of reaction center Chl of PSI, P700, after saturated pulse (SP) light illumination of the cyanobacterium Synechococcus elongatus PCC 7942 under steady-state photosynthetic conditions. Both P700 and NADPH were transiently oxidized after SP light illumination under CO2-depleted photosynthesis conditions. In contrast, the Chl fluorescence intensity transiently increased. Compared with the wild type, the increase in Chl fluorescence and the oxidations of P700 and NADPH were greatly enhanced in a mutant (Delta flv1/3) deficient in the genes encoding FLAVODIIRON 1 (FLV1) and FLV3 proteins even under high photosynthetic conditions. Furthermore, oxidation of Cyt f was also observed in the mutant. After SP light illumination, a transient suppression of O-2 evolution was also observed in Delta flv1/3. From these observations, we propose that the reduction in the plastquinone (PQ) pool suppresses linear electron flow at the Cyt b(6)/f complex, which we call the reduction-induced suppression of electron flow (RISE) in the PET system. The accumulation of the reduced form of PQ probably suppresses turnover of the Q cycle in the Cyt b(6)/f complex.

    OXFORD UNIV PRESS, 2016年07月, PLANT AND CELL PHYSIOLOGY, 57 (7), 1443 - 1453, 英語

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    研究論文(学術雑誌)

  • Daisuke Takagi, Shigeo Takumi, Masaki Hashiguchi, Takehiro Sejima, Chikahiro Miyake

    Photosystem I (PSI) photoinhibition suppresses plant photosynthesis and growth. However, the mechanism underlying PSI photoinhibition has not been fully clarified. In this study, in order to investigate the mechanism of PSI photoinhibition in higher plants, we applied repetitive short-pulse (rSP) illumination, which causes PSI-specific photoinhibition in chloroplasts isolated from spinach leaves. We found that rSP treatment caused PSI photoinhibition, but not PSII photoinhibition in isolated chloroplasts in the presence of O-2. However, chloroplastic superoxide dismutase and ascorbate peroxidase activities failed to protect PSI from its photoinhibition. Importantly, PSI photoinhibition was largely alleviated in the presence of methyl viologen, which stimulates the production of reactive oxygen species (ROS) at the stromal region by accepting electrons from PSI, even under the conditions where CuZn-superoxide dismutase and ascorbate peroxidase activities were inactivated by KCN. These results suggest that the ROS production site, but not the ROS production rate, is critical for PSI photoinhibition. Furthermore, we found that not only superoxide (O-2(-)) but also singlet oxygen (O-1(2)) is involved in PSI photoinhibition induced by rSP treatment. From these results, we suggest that PSI photoinhibition is caused by both O-2(-) and O-1(2) produced within the thylakoid membranes when electron carriers in PSI become highly reduced. Here, we show, to our knowledge, new insight into the PSI photoinhibition in higher plants.

    AMER SOC PLANT BIOLOGISTS, 2016年07月, PLANT PHYSIOLOGY, 171 (3), 1626 - 1634, 英語

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    研究論文(学術雑誌)

  • Daisuke Takagi, Kentaro Ifuku, Ken-ichi Ikeda, Kanako Ikeda Inoue, Pyoyun Park, Masahiro Tamoi, Hironori Inoue, Katsuhiko Sakamoto, Ryota Saito, Chikahiro Miyake

    Lipid-derived reactive carbonyl species (RCS) possess electrophilic moieties and cause oxidative stress by reacting with cellular components. Arabidopsis (Arabidopsis thaliana) has a chloroplast-localized alkenal/one oxidoreductase (AtAOR) for the detoxification of lipid-derived RCS, especially alpha,beta-unsaturated carbonyls. In this study, we aimed to evaluate the physiological importance of AtAOR and analyzed AtAOR (aor) mutants, including a transfer DNA knockout, aor (T-DNA), and RNA interference knockdown, aor (RNAi), lines. We found that both aor mutants showed smaller plant sizes than wild-type plants when they were grown under day/night cycle conditions. To elucidate the cause of the aor mutant phenotype, we analyzed the photosynthetic rate and the respiration rate by gas-exchange analysis. Subsequently, we found that both wildtype and aor (RNAi) plants showed similar CO2 assimilation rates; however, the respiration rate was lower in aor (RNAi) than in wild-type plants. Furthermore, we revealed that phosphoenolpyruvate carboxylase activity decreased and starch degradation during the night was suppressed in aor (RNAi). In contrast, the phenotype of aor (RNAi) was rescued when aor (RNAi) plants were grown under constant light conditions. These results indicate that the smaller plant sizes observed in aor mutants grown under day/night cycle conditions were attributable to the decrease in carbon utilization during the night. Here, we propose that the detoxification of lipid-derived RCS by AtAOR in chloroplasts contributes to the protection of dark respiration and supports plant growth during the night.

    AMER SOC PLANT BIOLOGISTS, 2016年04月, PLANT PHYSIOLOGY, 170 (4), 2024 - 2039, 英語

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    研究論文(学術雑誌)

  • Takehiro Sejima, Hitomi Hanawa, Ginga Shimakawa, Daisuke Takagi, Yuji Suzuki, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

    This study aims to elucidate the molecular mechanism for the transient increase in the O-2-uptake rate in tobacco (Nicotiana tabacum cv Xanthi) leaves after turning off actinic lights (ALs). The photosynthetic O-2 evolution rate reaches a maximum shortly after the onset of illumination with ALs and then decreases to zero in atmospheric CO2/O-2 conditions. After turning off the ALs, tobacco leaves show a transient increase in the O-2-uptake rate, the post-illumination transient O-2-uptake, and thereafter, the O-2-uptake rate decreases to the level of the dark-respiration rate. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], maintained a steady-state value distinct from the photosynthetic O-2-evolution rate. In high-[CO2] conditions, the photosynthetic O-2-evolution rate and Y(II) showed a parallel behavior, and the post-illumination transient O-2-uptake was suppressed. On the other hand, in maize leaves (a C4 plant), even in atmospheric CO2/O-2 conditions, Y(II) paralleled the photosynthetic O-2-evolution rate and the post-illumination transient O-2-uptake was suppressed. Hypothesizing that the post-illumination transient O-2-uptake is driven by C3 plant photorespiration in tobacco leaves, we calculated both the ribulose 1,5-bisphosphate carboxylase-and oxygenase-rates (Vc and Vo) from photosynthetic O-2-evolution and the post-illumination transient O-2-uptake rates. These values corresponded to those estimated from simultaneous chlorophyll fluorescence/O-2-exchange analysis. Furthermore, the H+-consumption rate for ATP synthesis in both photosynthesis and photorespiration, calculated from both Vc and Vo that were estimated from chlorophyll fluorescence/CO2-exchange analysis, showed a positive linear relationship with the dissipation rate of the electrochromic shift signal. Thus, these findings support our hypothesis.

    WILEY-BLACKWELL, 2016年02月, PHYSIOLOGIA PLANTARUM, 156 (2), 227 - 238, 英語

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    研究論文(学術雑誌)

  • Diversity in photosynthetic electron transport under [CO2]- limitation: the cyanobacterium Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O2-dependent alternative electron flow and non-photochemical quenching of chlorophyll f

    G. Shimakawa, S. Akimoto, Y. Ueno, A. Wada, K. Shaku, Y. Takahashi, C. Miyake

    2016年, Photosynth. Res., 130, 293 - 305, 英語

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    研究論文(学術雑誌)

  • Ginga Shimakawa, Seiji Akimoto, Yoshifumi Ueno, Ayumi Wada, Keiichiro Shaku, Yuichiro Takahashi, Chikahiro Miyake

    2016年, Photosynthesis Research, 1 - 13, 英語

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    研究論文(学術雑誌)

  • Aya Anegawa, Miwa Ohnishi, Daisuke Takagi, Chikahiro Miyake, Chizuko Shichijo, Kimitsune Ishizaki, Hidehiro Fukaki, Tetsuro Mimura

    A comprehensive analysis of the levels of primary metabolites in wild type (WT) and several auxin-signaling mutants namely, tir1, slr and arf7 arf19 of Arabidopsis thaliana has been performed using CE-MS, a technique particularly sensitive for the measurement of polar compounds. We first measured the levels of primary metabolites in shoots and roots, most of the analyzed metabolites were found to be quantitatively and qualitatively comparable in WT and three kinds of mutants (tir1, slr and arf7 arf19). Some amino acids such as GABA, Arg, Orn, Val, Thr, Leu and Ile exhibited a unique pattern of distribution between shoots and roots in both WT and the mutants. On the other hand, the mutant slr showed a quite different pattern of metabolites measured in the present study. Subsequently, the responses of primary metabolites to a short-term (60 min) application of exogenous IAA (10(-7), 10(-8) M) in WT and the mutants were characterized. Due to IAA treatments, some amino acids such as GABA in WT roots and Gly and Ala in WT shoots were altered, but not in the mutants. Gln was altered in slr shoots by 10(-7) M IAA treatment. Levels of G6P from the glycolic pathway were altered in WT roots and those of 2PG, 3PG were altered in tir1 shoots in response to IAA treatments. The levels of succinate in TCA cycle were altered by IAA treatments in WT shoots but not in the mutants. IAA treatment inhibited the respiration in WT roots. The suppression of respiration might account for the IAA-dependent alteration of some metabolites. Difference of auxin responses between WT and auxin-signaling mutants suggests that some metabolic processes are under IAA control.

    JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, 2015年03月, PLANT BIOTECHNOLOGY, 32 (1), 65 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga Shimakawa, Keiichiro Shaku, Akiko Nishi, Ryosuke Hayashi, Hiroshi Yamamoto, Katsuhiko Sakamoto, Amane Makino, Chikahiro Miyake

    This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly after the onset of actinic illumination. Thereafter, Y(II) transiently decreases concomitantly with a decrease in the photosynthetic oxygen evolution rate and then recovers to a rate that is close to the initial maximum. These results show that CO2 limitation suppresses photosynthesis and induces AEF. In contrast to the wild type, Synechocystis sp. PCC 6803 mutants deficient in the genes encoding FLAVODIIRON2 (FLV2) and FLV4 proteins show no recovery of Y(II) after prolonged illumination. However, Synechocystis sp. PCC 6803 mutants deficient in genes encoding proteins functioning in photorespiration show AEF activity similar to the wild type. In contrast to Synechocystis sp. PCC 6803, the cyanobacterium Synechococcus elongatus PCC 7942 has no FLV proteins with high homology to FLV2 and FLV4 in Synechocystis sp. PCC 6803. This lack of FLV2/4 may explain why AEF is not induced under CO2-limited photosynthesis in S. elongatus PCC 7942. As the glutathione S-transferase fusion protein overexpressed in Escherichia coli exhibits NADH-dependent oxygen reduction to water, we suggest that FLV2 and FLV4 mediate oxygen-dependent AEF in Synechocystis sp. PCC 6803 when electron acceptors such as CO2 are not available.

    AMER SOC PLANT BIOLOGISTS, 2015年02月, PLANT PHYSIOLOGY, 167 (2), 472 - U732, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hasunuma Tomohisa, Matsuda Mami, Senga Youhei, Aikawa Shimpei, Toyoshima Masakazu, Shimakawa Ginga, Miyake Chikahiro, Kondo Akihiko

    2014年12月31日, BIOTECHNOLOGY FOR BIOFUELS, 7

    [査読有り]

  • Ginga Shimakawa, Tomohisa Hasunuma, Akihiko Kondo, Mami Matsuda, Amane Makino, Chikahiro Miyake

    We tested the hypothesis that inducing photosynthesis in cyanobacteria requires respiration. A mutant deficient in glycogen phosphorylase ( increment GlgP) was prepared in Synechocystis sp. PCC 6803 to suppress respiration. The accumulated glycogen in Delta GlgP was 250-450% of that accumulated in wild type (WT). The rate of dark respiration in Delta GlgP was 25% of that in WT. In the dark, P700(+) reduction was suppressed in Delta GlgP, and the rate corresponded to that in (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone)-treated WT, supporting a lower respiration rate in increment GlgP. Photosynthetic O-2-evolution rate reached a steady-state value much slower in increment GlgP than in WT. This retardation was solved by addition of d-glucose. Furthermore, we found that the contents of Calvin cycle intermediates in increment GlgP were lower than those in WT under dark conditions. These observations indicated that respiration provided the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis.

    TAYLOR & FRANCIS LTD, 2014年12月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 78 (12), 1997 - 2007, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takehiro Sejima, Daisuke Takagi, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

    Under field conditions, the leaves of plants are exposed to fluctuating light, as observed in sunfleck. The duration and frequency of sunfleck, which is caused by the canopy being blown by the wind, are in the ranges from 0.2 to 50 s, and from 0.004 to 1 Hz, respectively. Furthermore, > 60% of the sunfleck duration ranges from 0.2 to 0.8 s. In the present research, we analyzed the effects of repetitive illumination by short-pulse (SP) light of sunflower leaves on the photosynthetic electron flow. The duration of SP light was set in the range from 10 to 300 ms. We found that repetitive illumination with SP light did not induce the oxidation of P700 in PSI, and mainly inactivated PSI. Increases in the intensity, duration and frequency of SP light enhanced PSI photoinhibition. PSI photoinhibition required the presence of O-2. The inactivation of PSI suppressed the net CO2 assimilation. On the other hand, the increase in the oxidized state of P700 suppressed PSI inactivation. That is, PSI with a reduced reaction center would produce reactive oxygen species (ROS) by SP light, leading to PSI photodamage. This mechanism probably explains the PSI photodamage induced by constant light.

    OXFORD UNIV PRESS, 2014年06月, PLANT AND CELL PHYSIOLOGY, 55 (6), 1184 - 1193, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga Shimakawa, Mayumi Suzuki, Eriko Yamamoto, Ryota Saito, Tatsuya Iwamoto, Akiko Nishi, Chikahiro Miyake

    In the present paper, we review the toxicity of sugar- and lipid-derived RCs (reactive carbonyls) and the RC-scavenging systems observed in photosynthetic organisms. Similar to heterotrophs, photosynthetic organisms are exposed to the danger of RCs produced in sugar metabolism during both respiration and photosynthesis. RCs such as methylglyoxal and acrolein have toxic effects on the photosynthetic activity of higher plants and cyanobacteria. These toxic effects are assumed to occur uniquely in photosynthetic organisms, suggesting that RC-scavenging systems are essential for their survival. The aldo-keto reductase and the glyoxalase systems mainly scavenge sugar-derived RCs in higher plants and cyanobacteria. 2-Alkenal reductase and alkenal/alkenone reductase catalyse the reduction of lipid-derived RCs in higher plants. In cyanobacteria, medium-chain dehydrogenases/reductases are the main scavengers of lipid-derived RCs.

    PORTLAND PRESS LTD, 2014年04月, BIOCHEMICAL SOCIETY TRANSACTIONS, 42, 543 - 547, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Takagi, Hironori Inoue, Mizue Odawara, Ginga Shimakawa, Chikahiro Miyake

    Sugar-derived reactive carbonyls (RCs), including methylglyoxal (MG), are aggressive by-products of oxidative stress known to impair the functions of multiple proteins. These advanced glycation end-products accumulate in patients with diabetes mellitus and cause major complications, including arteriosclerosis and cardiac insufficiency. In the glycolytic pathway, the equilibration reactions between dihydroxyacetone phosphate and glyceraldehyde 3-phosphate (GAP) have recently been shown to generate MG as a by-product. Because plants produce vast amounts of sugars and support the same reaction in the Calvin cycle, we hypothesized that MG also accumulates in chloroplasts. Incubating isolated chloroplasts with excess 3-phosphoglycerate (3-PGA) as the GAP precursor drove the equilibration reaction toward MG production. The rate of oxygen (O-2) evolution was used as an index of 3-PGA-mediated photosynthesis. The 3-PGA- and time-dependent accumulation of MG in chloroplasts was confirmed by HPLC. In addition, MG production increased with an increase in light intensity. We also observed a positive linear relationship between the rates of MG production and O-2 evolution (R = 0.88; P< 0.0001). These data provide evidence that MG is produced by the Calvin cycle and that sugar-derived RC production is inevitable during photosynthesis. Furthermore, we found that MG production is enhanced under high-CO2 conditions in illuminated wheat leaves.

    OXFORD UNIV PRESS, 2014年02月, PLANT AND CELL PHYSIOLOGY, 55 (2), 333 - 340, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 齊藤 亮太, 伊福 健太郎, 池田 健一, 井上 加奈子, 三宅 親弘

    一般社団法人 日本土壌肥料学会, 2014年, 日本土壌肥料学会講演要旨集, 60 (0), 288 - 288, 日本語

    [査読有り]

  • Ryosuke Hayashi, Ginga Shimakawa, Keiichiro Shaku, Satoko Shimizu, Seiji Akimoto, Hiroshi Yamamoto, Katsumi Amako, Toshio Sugimoto, Masahiro Tamoi, Amane Makino, Chikahiro Miyake

    To determine whether alternative electron flow (AEF) can replace the photosynthetic electron flow in cyanobacteria, we used an open O-2-electrode system to monitor O-2-exchange over a long period. In air-grown Synechocystis sp. PCC 6803 (S. 6803 (WT)), the quantum yield of PSII, Y(II), held even after photosynthesis was suppressed by CO2 shortage. The S. 6803 mutant, deficient in flavodiiron (FLV) proteins 1 and 3, showed the same phenotype as S. 6803(WT). In contrast, Y(II) decreased in Synechococcus sp. PCC 7942 (S. 7942). These results suggest that AEF functioned as the Y(II) in S. 6803 and replaced the photosynthetic electron flux. In contrast, the activity of AEF in S. 7942 was lower. The affinity of AEF for O-2 in S. 6803 did not correspond to those of FLVs in bacteria or terminal oxidases in respiration. AEF might be driven by photorespiration.

    TAYLOR & FRANCIS LTD, 2014年, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 78 (3), 384 - 393, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryota Saito, Ginga Shimakawa, Akiko Nishi, Tatsuya Iwamoto, Katsuhiko Sakamoto, Hiroshi Yamamoto, Katsumi Amako, Amane Makino, Chikahiro Miyake

    In Arabidopsis thaliana, the aldo-keto reductase (AKR) family includes four enzymes (The AKR4C subfamily: AKR4C8, AKR4C9, AKR4C10, and AKR4C11). AKR4C8 and AKR4C9 might detoxify sugar-derived reactive carbonyls (RCs). We analyzed AKR4C10 and AKR4C11, and compared the enzymatic functions of the four enzymes. Modeling of protein structures based on the known structure of AKR4C9 found an (alpha/beta)(8)-barrel motif in all four enzymes. Loop structures (A, B, and C) which determine substrate specificity, differed among the four. Both AKR4C10 and AKR4C11 reduced methylglyoxal. AKR4C10 reduced triose phosphates, dihydroxyacetone phosphate (DHAP), and glyceraldehydes 3-phosphate (GAP), the most efficiently of all the AKR4Cs. Acrolein, a lipid-derived RC, inactivated the four enzymes to different degrees. Expression of the AKR4C genes was induced under high-[CO2] and high light, when photosynthesis was enhanced and photosynthates accumulated in the cells. These results suggest that the AKR4C subfamily contributes to the detoxification of sugar-derived RCs in plants.

    TAYLOR & FRANCIS LTD, 2013年10月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77 (10), 2038 - 2045, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga Shimakawa, Tatsuya Iwamoto, Tomohito Mabuchi, Ryota Saito, Hiroshi Yamamoto, Katsumi Amako, Toshio Sugimoto, Amane Makino, Chikahiro Miyake

    In this study, we sought to determine whether and how an alpha,beta-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200 mu M acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO2-dependent O-2 evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200 mu M acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.

    TAYLOR & FRANCIS LTD, 2013年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77 (8), 1655 - 1660, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ginga Shimakawa, Mayumi Suzuki, Eriko Yamamoto, Akiko Nishi, Ryota Saito, Katsuhiko Sakamoto, Hiroshi Yamamoto, Amane Makino, Chikahiro Miyake

    To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls (RCs) in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803), we selected proteins from S. 6803 based on amino-acid (AA) sequence similarities with proteins from Arabidopsis thaliana, and characterized the properties of the GST-fusion proteins expressed. Slr0942 catalyzed the aldo-keto reductase (AKR) reaction scavenging mainly sugar-derived RCs, methylglyoxal (MG). Slr1192 is the medium-chain dehydrogenase/redutase (MDR). It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. Slr0315 is a short-chain dehydrogenase/redutase (SDR), and it catalyzed only the reduction of MG in the AKR reaction. Slr0381 catalyzed the conversion of hemithioacetal to S-lactoylglutahione (SLG) in the glyoxalase (GLX) 1 reaction. Sll1019 catalyzed the conversion of SLG to glutathione and lactate in the GLX2 reaction. GLX1 and GLX2 compose the glyoxalase system, which scavenges MG. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems.

    Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2013年, Bioscience, Biotechnology and Biochemistry, 77 (12), 2441 - 2448, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Miyake C, Suzuki Y, Yamamoto H, Amako K, Makino A

    IF = 1.017

    2012年12月, Soil Science and Plant Nutrition, 58, 718 - 727, 英語

    [査読有り]

    研究論文(学術雑誌)

  • O2 supports 3-phosphoglycerate-dependent O2 evolution in chloroplasts from spinach leaves

    Daisuke Takagi, Hiroshi Yamamoto, Katsumi Amako, Amane Makino, Toshio Sugimoto, Chikahiro Miyake

    2012年, Soil Science and Plant Nutrition, 58 (4), 462 - 468, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Daisuke Takagi, Hiroshi Yamamoto, Katsumi Amako, Amane Makino, Toshio Sugimoto, Chikahiro Miyake

    We tested the hypothesis that the Mehler-ascorbate peroxidase (MAP) pathway supports 3-phosphoglycerate (PGA)-dependent oxygen (O-2) evolution using intact chloroplasts. Lowering O-2 concentration (<1 mu M) suppressed PGA-dependent O-2 evolution rate. High O-2 concentration (about 250 mM) enhanced the electron fluxes in Photosystem II (PSII). Also, high O-2 concentration oxidized both Q(A) in PSII and Cyt f in thylakoid membranes. These results indicated that the MAP pathway stimulated photosynthetic electron transport. Furthermore, electrochromic shift signal was also increased at high O-2 concentration, compared to low O-2 concentration. Non-photochemical quenching of chlorophyll fluorescence was also enhanced at high O-2 concentration. These data support our hypothesis that the MAP pathway functioned in intact chloroplasts and accelerated PGA-dependent O-2 evolution by inducing Delta pH formation to produce and supply adenosine triphosphate (ATP) to the conversion reaction of PGA to glyceraldehyde 3-phosphate through 1,3-diphosphoglycerate in chloroplasts.

    TAYLOR & FRANCIS LTD, 2012年, SOIL SCIENCE AND PLANT NUTRITION, 58 (4), 462 - 468, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryota Saito, Hiroshi Yamamoto, Amane Makino, Toshio Sugimoto, Chikahiro Miyake

    We elucidated the metabolism of methylglyoxal (MG) in chloroplasts of higher plants. Spinach chloroplasts showed MG-dependent NADPH oxidation because of aldo-keto reductase (AKR) activity. K-m for MG and V-max of AKR activity were 6.5 mM and 3.3 mu mol NADPH (mg Chl)(-1) h(-1), respectively. Addition of MG to illuminated chloroplasts induced photochemical quenching (Qp) of Chl fluorescence, indicating that MG stimulated photosynthetic electron transport (PET). Furthermore, MG enhanced the light-dependent uptake of O-2 into chloroplasts. After illumination of chloroplasts, accumulation of H2O2 was observed. K-m for MG and V-max of O-2 uptake were about 100 mu M and 200 mu mol O-2 (mg Chl)(-1) h(-1), respectively. MG-dependent O-2 uptake was inhibited by 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). Under anaerobic conditions, the Qp of Chl fluorescence was suppressed. These results indicate that MG was reduced as a Hill oxidant by the photosystem I (PSI), and that O-2 was reduced to O-2 by the reduced MG. In other words, MG produced in chloroplasts is preferentially reduced by PSI rather than through AKR. This triggers a type of oxidative stress that may be referred to as 'plant diabetes', because it ultimately originates from a common metabolite of the primary pathways of sugar anabolism and catabolism.

    WILEY-BLACKWELL, 2011年09月, PLANT CELL AND ENVIRONMENT, 34 (9), 1454 - 1464, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoshi Kubo, Takehiro Masumura, Yuhi Saito, Hiroshi Fukayama, Yuji Suzuki, Toshio Sugimoto, Amane Makino, Katsumi Amako, Chikahiro Miyake

    We investigated the regulation mechanism of cyclic electron flow around photosystem I (CEF-PSI) in the rice leaves which suffered from photosystem II (PSII) photoinhibition. High-light (HL) treatment [2000 mu mol photon m-2 s-1 at 0% carbon dioxide (CO2), 2% oxygen (O2) and 25 degrees C] of rice leaves decreased both the maximum quantum efficiency of PSII (Fv/Fm) and the light-dependent O2-evolution rate [V(O2)]. High-light treatment did not affect the relative electron flux in PSI [phi(PSI) x PFD]. In non-treated leaves, increasing in the photon flux density (PFD) enhanced V(O2), phi(PSI) x PFD and the ratio of oxidized P700 to total P700 [(P700+)/(P700)total]. phi(PSI) x PFD continued to increase even after the saturation of V(O2) against PFD. These results suggested that the electrons not used for the major electron sink, photosynthetic carbon reduction-cycle, did turnover in PSI, that is, CEF-PSI functioned at a higher PFD. High-light treatments did not affect the activity of CEF-PSI and increased (P700+)/(P700)total in the lower PFDs, compared to non-treated leaves. The activity of CEF-PSI depends on the amount of oxidized PQ. Photoinhibition of PSII suppressed electron influx from PSII to photosynthetic linear electron transport. The enhanced (P700+)/(P700)total suggested the increase in the ratio of oxidized plastoquinone (PQ) to total PQ, which supported the activity of CEF-PSI in the photoinhibited leaves.

    WILEY-BLACKWELL, 2011年02月, SOIL SCIENCE AND PLANT NUTRITION, 57 (1), 105 - 113, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Satoshi Kubo, Takehiro Masumura, Yuhi Saito, Hiroshi Fukayama, Yuji Suzuki, Toshio Sugimoto, Amane Makino, Katsumi Amako, Chikahiro Miyake

    We investigated the regulation mechanism of cyclic electron flow around photosystem I (CEF-PSI) in the rice leaves which suffered from photosystem II (PSII) photoinhibition. High-light (HL) treatment [2000 mu mol photon m-2 s-1 at 0% carbon dioxide (CO2), 2% oxygen (O2) and 25 degrees C] of rice leaves decreased both the maximum quantum efficiency of PSII (Fv/Fm) and the light-dependent O2-evolution rate [V(O2)]. High-light treatment did not affect the relative electron flux in PSI [phi(PSI) x PFD]. In non-treated leaves, increasing in the photon flux density (PFD) enhanced V(O2), phi(PSI) x PFD and the ratio of oxidized P700 to total P700 [(P700+)/(P700)total]. phi(PSI) x PFD continued to increase even after the saturation of V(O2) against PFD. These results suggested that the electrons not used for the major electron sink, photosynthetic carbon reduction-cycle, did turnover in PSI, that is, CEF-PSI functioned at a higher PFD. High-light treatments did not affect the activity of CEF-PSI and increased (P700+)/(P700)total in the lower PFDs, compared to non-treated leaves. The activity of CEF-PSI depends on the amount of oxidized PQ. Photoinhibition of PSII suppressed electron influx from PSII to photosynthetic linear electron transport. The enhanced (P700+)/(P700)total suggested the increase in the ratio of oxidized plastoquinone (PQ) to total PQ, which supported the activity of CEF-PSI in the photoinhibited leaves.

    WILEY-BLACKWELL, 2011年02月, SOIL SCIENCE AND PLANT NUTRITION, 57 (1), 105 - 113, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Functional Incorporation of Sorghum Small Subunit Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice.

    石川 知恵, 畠中 知子, 三十尾 修司, 三宅 親弘, 深山 浩

    2011年, Plant Physiol., 156, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YUJI SUZUKI, TOMONORI KIHARA-DOI, TETSU KAWAZU, CHIKAHIRO MIYAKE, AMANE MAKINO

    2010年08月, Plant, Cell and Environment, 33 (8), 1314 - 1323

    [査読有り]

  • Chikahiro Miyake, Katsumi Amako, Naomasa Shiraishi, Toshio Sugimoto

    Responses of the reductionoxidation level of plasto-quinone (PQ) in the photosynthetic electron transport (PET) system of chloroplasts to growth light intensity were evaluated in tobacco plants. Plants grown in low light (150mol photons m(2) s(1)) (LL plants) were exposed to a high light intensity (1,100mol photons m(2)s(1)) for 1d. Subsequently, the plants exposed to high light (LH plants) were returned back again to the low light condition: these plants were designated as LHL plants. Both LH and LHL plants showed higher values of non-photochemical quenching of Chl fluorescence (NPQ) and the fraction of open PSII centers (qL), and lower values of the maximum quantum yield of PSII in the dark (F(v)F(m)), compared with LL plants. The dependence of qL on the quantum yield of PSII [(PSII)] in LH and LHL plants was higher than that in LL plants. To evaluate the effect of an increase in NPQ and decrease in F(v)F(m) on qL, we derived an equation expressing qL in relation to both NPQ and F(v)F(m), according to the lake model of photoexcitation of the PSII reaction center. As a result, the heat dissipation process, shown as NPQ, did not contribute greatly to the increase in qL. On the other hand, decreased F(v)F(m) did contribute to the increase in qL, i.e. the enhanced oxidation of PQ under photosynthesis-limited conditions. Thylakoid membranes isolated from LH plants, having high qL, showed a higher tolerance against photoinhibition of PSII, compared with those from LL plants. We propose a plastoquinone oxidation system (POS), which keeps PQ in an oxidized state by suppressing the accumulation of electrons in the PET system in such a way as to regulate the maximum quantum yield of PSII.

    OXFORD UNIV PRESS, 2009年04月, PLANT AND CELL PHYSIOLOGY, 50 (4), 730 - 743, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants

    Tomohisa Hasunuma, Akihiko Kondo, Chikahiro Miyake

    2009年03月, Plant Biotechnology, 26 (1), 39 - 46, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kunio Ido, Kentaro Ifuku, Yumiko Yamamoto, Seiko Ishihara, Akio Murakami, Keiji Takabe, Chikahiro Miyake, Fumihiko Sato

    2009年, Biochemica et Biophysica Acta 1787, 873-88, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Overexpression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production

    蓮沼 誠久, 武野 真也, 林 俊介, 千代 真由美, 馬場 健史, 吉村 智美, 富澤 健一, 福崎 英一郎, 三宅 親弘

    2008年05月, Journal of Bioscience and Biotechnology, Vol 105. No. 5, pp. 518-526, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 4-Ketoantheraxanthin, a novel carotenoid produced by the combination of the bacterial enzyme b-carotene ketolase CrtW and endogeneous carotenoid biosynthetic enzymes in hihger plants

    新藤 一敏, 蓮沼 誠久, ASAGI Emiko, SANO Aya, HOTTA Eri, MINEMURA Noriko, 三宅 親弘, 眞岡 孝至, 三沢 典彦

    2008年03月, Tetrahedron Letters, Vol 49. No. 20, pp. 3294-3296, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Kunio Ido, Kentaro Ifuku, Seiko Ishihara, Yumiko Yamamoto, Chikahiro Miyake, Fumihiko Sato

    2008年, Photosynthesis Energy from the Sun, 5, 605 - 608, 英語

    研究論文(国際会議プロシーディングス)

  • Yamamoto Hiroshi, Kato Hideki, Shinzaki Yuki, Horiguchi Sayaka, Shikanai Toshiharu, Hase Toshiharu, Endo Tsuyoshi, Nishioka Minori, Makino Amane, Tomizawa Ken-ichi, Miyake Chikahiro

    2007年, PLANT AND CELL PHYSIOLOGY, 48, S96

    [査読有り]

  • Chikahiro Miyake, Sayaka Horiguchi, Amane Makino, Yuki Shinzaki, Hiroshi Yamamoto, Ken-Ichi Tomizawa

    We tested the hypothesis that plants grown under high light intensity (HL-plants) had a large activity of cyclic electron flow around PSI (CEF-PSI) compared with plants grown under low light (LL-plants). To evaluate the activity of CEF-PSI, the relationships between photosynthesis rate, quantum yields of both PSII and PSI, and Chl fluorescence parameters were analyzed simultaneously in intact leaves of tobacco plants which had been grown under different light intensities (150 and 1,100 μmol photons m-2 s-1, respectively) and with different amounts of nutrients supplied. HL-plants showed a larger value of non-photochemical quenching (NPQ) of Chl fluorescence at the limited activity of photosynthetic linear electron flow. Furthermore, HL-plants had a larger activity of CEF-PSI than LL-plants. These results suggested that HL-plants dissipated the excess photon energy through NPQ by enhancing the ability of CEF-PSI to induce acidification of the thylakoid lumen. JSPP © 2005.

    2005年11月, Plant and Cell Physiology, 46 (11), 1819 - 1830, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 三宅親弘, 牧野 周

    2003年07月, 化学と生物, 41 (7), 478 - 487, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Amane Makino, Chikahiro Miyake, Akiho Yokota

    Changes in chlorophyll fluorescence, P700+-absorbance and gas exchange during the induction phase and steady state of photosynthesis were simultaneously examined in rice (Oryza sativa L.), including the rbcS antisense plants. The quantum yield of photosystem II (ΦPSII) increased more rapidly than CO2 assimilation in 20% O2. This rapid increase in ΦPSII resulted from the electron flux through the water-water cycle (WWC) because of its dependency on O2. The electron flux of WWC reached a maximum just after illumination, and rapidly generated non-photochemical quenching (NPQ). With increasing CO2 assimilation, the electron flux of WWC and NPQ decreased. In 2% O2, WWC scarcely operated and ΦPSI was always higher than ΦPSII. This suggested that cyclic electron flow around PSI resulted in the formation of NPQ, which remained at higher levels in 2% O2. The electron flux of WWC in the rbcS antisense plants was lower, but these plants always showed a higher NPQ. This was also caused by the operation of the cyclic electron flow around PSI because of a higher ratio of ΦPSI/ΦPSII, irrespective of O2 concentration. The results indicate that WWC functions as a starter of photosynthesis by generating ΔpH across thylakoid membranes for NPQ formation, supplying ATP for carbon assimilation. However, WWC does not act to maintain a high NPQ, and ΦPSII is down-regulated by ΔpH generated via the cyclic electron flow around PSI.

    Japanese Society of Plant Physiologists, 2002年09月01日, Plant and Cell Physiology, 43 (9), 1017 - 1026, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • 作物生産における酸化ストレス—障害機構,耐性機構,危険予知による回避—

    鈴木 雄二, 小林 優, 小林 佑理子, 大津(大鎌) 直子, 伊福 健太郎, 三宅 親弘

    一般社団法人 日本土壌肥料学会, 2019年, 日本土壌肥料学雑誌, 90 (4), 273 - 278, 日本語

  • イネにおけるP700吸光パラメーターによる水ストレス診断

    和田慎也, 和田慎也, 鈴木雄二, 鈴木雄二, 高木大輔, 高木大輔, 三宅親弘, 三宅親弘, 牧野周, 牧野周

    2018年08月29日, 日本土壌肥料学会講演要旨集, 64, 56, 日本語

  • Ginga Shimakawa, Ayaka Kohara, Chikahiro Miyake

    Reactive carbonyls (RCs), which are inevitably produced during respiratory and photosynthetic metabolism, have the potential to cause oxidative damage to photosynthetic organisms. Previously, we proposed a scavenging model for RCs in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803). In the current study, we constructed mutants deficient in the enzymes medium-chain dehydrogenase/reductase (ΔMDR) and aldo-keto reductase (ΔAKR) to investigate their contributions to RC scavenging in vivo. We found that treatment with the lipid-derived RC acrolein causes growth inhibition and promotes greater protein carbonylation in ΔMDR, compared with the wild-type and ΔAKR. In both ΔMDR and ΔAKR, photosynthesis is severely inhibited in the presence of acrolein. These results suggest that these enzymes function as part of the scavenging systems for RCs in S. 6803 in vivo.

    Wiley Blackwell, 2018年03月01日, FEBS Letters, 592 (6), 1010 - 1019, 英語

    速報,短報,研究ノート等(学術雑誌)

  • 光化学系IIシトクロムb559におけるアンチマイシンAの影響

    高木大輔, 伊福健太郎, 西村太志, 三宅親弘

    2018年03月, 第59回 日本植物生理学会年会, 日本語

    研究発表ペーパー・要旨(全国大会,その他学術会議)

  • NADPH-dependent alkenal/one oxidoreductase (AOR) supports the growth and protect from oxidative stress in Arabidopsis thaliana

    Takagi Daisuke, Kentaro Ifuku, Hironori Inoue, Masahiro Tamoi, Ken-ichi Ikeda, Kanako Ikeda Inoue, Hiroshi Fukayama, Chikahiro Miyake

    2015年03月, 第56回日本植物生理学会年会, 日本語

    研究発表ペーパー・要旨(全国大会,その他学術会議)

  • これまで欠けていた、速度論的評価に基づく、 オルタナティブ・エレクトロン・フロー活性の比較と光合成におけるO2の役割 ~生理的な意味が見える、本丸へ挑む~

    高木 大輔, 三宅 親弘

    2014年, 光合成研究, 24, 日本語

    [査読有り]

    記事・総説・解説・論説等(学術雑誌)

  • イネ生葉におけるMAP-pathwayによる光合成誘導メカニズムの解析

    瀬島健裕, 鈴木雄二, 高木大輔, 山本宏, 杉本敏男, 尼子克己, 牧野周, 三宅親弘

    2013年03月, 第54回日本植物生理学会, 日本語

  • タバコrbcL遺伝子のポプラrbcL遺伝子への置換によるhybrid Rubiscoの形成

    鈴木 直紀, 関谷 卓生, 表谷 拓郎, 鈴木 雄二, 山本 宏, 三宅 親弘, 牧野 周

    2012年03月, 第53回日本植物生理学会, 日本語

  • Cyanobacterium Synechocystis sp. PCC6803 におけるO2存性オルタナティブ・エレクトロン・フロー (AEF)の生理的役割

    林 良祐, 清水 聡子, 山本 宏, 藍川 晋平, 蓮沼 誠久, 秋本 誠志, 近藤 昭彦, 三宅 親弘

    2012年03月, 第53回日本植物生理学会, 日本語

  • 植物葉緑体メチルグリオキサール代謝メカニズムの解明 メチルグリオキサールは内在的酸素光還元メディエーターである

    斉藤 亮太, 山本 宏, 牧野 周, 杉本 敏男, 三宅 親弘

    2011年09月, 第75回日本植物学会, 日本語

  • 10 葉緑体におけるMethylglyoxal解毒機構の解明1 : 植物はなぜ糖尿病にならないのか(関西支部講演会,2010年度各支部会講演要旨)

    岩本 達弥, 斉藤 亮太, 久保 智史, 杉本 敏男, 伊福 健太郎, 佐藤 文彦, 牧野 周, 三宅 親弘

    一般社団法人日本土壌肥料学会, 2011年08月, 日本土壌肥料学会講演要旨集, (57), 日本語

    研究発表ペーパー・要旨(全国大会,その他学術会議)

  • 高等植物葉緑体での3-ホスホグリセリン酸に依存した光合成における酸素の重要性 ATP/NADPH=1 でさえリニアエレクトロンフローでは満たせられない

    高木 大輔, 山本 宏, 尼子 克己, 三宅 親弘

    2011年, 第75回日本植物学会, 日本語

  • シロイヌナズナでのAEF(the Water-Water Cycle and Cyclic Electron Flow around PSI)の強光順化への応答:O2-dependent electron flowはelectron sinkとして機能し、PQ酸化およびNPQ誘導に貢献する

    桑原亮, 尼子克己, 山本 宏, 鹿内利治, 杉本敏男, 鈴木雄二, 牧野 周, 三宅親弘

    2010年, 日本農芸化学会2010年度関西支部大会, 日本語

  • スクリーニングにより選抜された塩・強光耐性ユーカリの光合成特性解析:–熱散逸機構とその誘導メカニズムについて-

    三宅 親弘, 宮澤 真一, 岩前 智子

    日本森林学会, 2008年, 日本森林学会大会発表データベース, 119 (0), 276 - 276, 日本語

講演・口頭発表等

  • 蛍光分光法による酸素発生型光合成生物における光エネルギー調節機構に関する研究

    植野 嘉文, 嶋川 銀河, 藍川 晋平, 三宅 親弘, 秋本 誠志

    2018年光化学討論会, 2018年09月, 日本語, 関西学院大学・上ケ原キャンパス, 国内会議

    ポスター発表

  • 単細胞緑藻Chlorella variabilis における低CO2条件での光捕集機能調整

    植野 嘉文, 嶋川 銀河, 三宅 親弘, 秋本 誠志

    第9回日本光合成学会年会, 2018年, 日本語, 仙台, 国内会議

    ポスター発表

  • 絶対蛍光強度測定による緑藻Chlorella variabilis におけるCO2条件に依存した光捕集機能調節の解明

    植野 嘉文, 嶋川 銀河, 藍川 晋平, 三宅 親弘, 秋本 誠志

    光合成セミナー2018:反応中心と色素系の多様性, 2018年, 日本語, 神戸, 国内会議

    ポスター発表

  • 緑藻Chlamydomonas reinhardtii におけるCO2 に対する光捕集機能変化

    植野 嘉文, 嶋川 銀河, 三宅 親弘, 秋本 誠志

    第58回日本植物生理学会年会, 2017年03月, 日本語, 鹿児島大学郡元キャンパス, 国内会議

    ポスター発表

  • 高CO2 条件から低CO2 条件への移行に伴うシアノバクテリアの光合成初期過程の変化

    藤本 かおり, 嶋川 銀河, 三宅 親弘, 秋本 誠志

    第58回日本植物生理学会年会, 2017年03月, 日本語, 鹿児島大学郡元キャンパス, 国内会議

    ポスター発表

  • 異なる二酸化炭素濃度下で培養されたシアノバクテリアにおける光エネルギー捕集過程

    池田 志保, 藍川 晋平, 嶋川 銀河, 三宅 親弘, 近藤 昭彦, 秋本 誠志

    第58回日本植物生理学会年会, 2017年03月, 日本語, 鹿児島大学郡元キャンパス, 国内会議

    ポスター発表

  • 高濃度二酸化炭素条件下で培養したシアノバクテリアの光応答

    藤本 かおり, 嶋川 銀河, 三宅 親弘, 秋本 誠志

    第10回分子科学討論会2016神戸, 2016年09月, 日本語, 神戸ファッションマート, 国内会議

    ポスター発表

  • 絶対蛍光強度測定による緑藻Chlamydomonas reinhardtiiにおける光捕集機能変化の解明

    植野 嘉文, 嶋川 銀河, 三宅 親弘, 秋本 誠志

    第24回 「光合成セミナー2016:反応中心と色素系の多様性」, 2016年07月, 日本語, 龍谷大学深草キャンパス, 国内会議

    ポスター発表

  • 77 Kにおける蛍光の絶対強度測定 −シアノバクテリアの光応答−

    藤本 かおり, 嶋川 銀河, 三宅 親弘, 秋本 誠志

    第24回 「光合成セミナー2016:反応中心と色素系の多様性」, 2016年07月, 日本語, 龍谷大学深草キャンパス, 国内会議

    ポスター発表

  • 高等植物におけるalkenal/one oxidoreductase (AOR)の生理学的解析 植物も糖尿病を発症する

    齋藤 亮太, 伊福 健太郎, 池田 健一, 池田 加奈子, 三宅 親弘

    日本土壌肥料学会関西支部会, 2013年, 日本語, 国内会議

    口頭発表(一般)

共同研究・競争的資金等の研究課題