研究者紹介システム
| 笹崎 晋史 |  |
| ササザキ シンジ | |
| 大学院農学研究科 資源生命科学専攻 | |
| 准教授 | |
| 農学関係 |
研究者情報
所属
【主配置】
大学院農学研究科 資源生命科学専攻【配置】
農学部 資源生命科学科
学位
授業科目
- 生物学実験１, 国際教養教育院, 2021
- 生物学実験１, 国際教養教育院, 2021
- 生物学実験１, 国際教養教育院, 2021
- 生物学実験２, 国際教養教育院, 2021
- 生物学実験２, 国際教養教育院, 2021
- 生物学実験２, 国際教養教育院, 2021
- 特定課題演習 I-1, 大学院農学研究科, 2021
- 課題開発演習, 大学院農学研究科, 2021
- 資源生命科学プレゼンテーション演習Ⅰ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-1, 大学院農学研究科, 2021
- 動物ゲノム学, 大学院農学研究科, 2021
- 特定課題演習 I-2, 大学院農学研究科, 2021
- 資源生命科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 先端遺伝育種論, 大学院農学研究科, 2021
- Global Topics in Animal Science, 農学部, 2021
- 高度教養セミナー農学部応用動物学, 農学部, 2021
- 卒業研究, 農学部, 2021
- 応用動物学実験, 農学部, 2021
- 外国書講読Ａ, 農学部, 2021
- 資源生命科学入門Ⅰ－１, 農学部, 2021
- 初年次セミナー（応用動物学コース）, 農学部, 2021
- 資源生命科学入門Ⅰ－２, 農学部, 2021
- 高度教養セミナー農学部応用動物学入門, 農学部, 2021
- 高度教養セミナー農学部応用動物学入門, 農学部, 2021
- 応用動物学各論, 農学部, 2021
- 家畜ゲノム学１, 農学部, 2021
- 家畜ゲノム学, 農学部, 2021
- 家畜ゲノム学２, 農学部, 2021
ジャンル
コメントテーマ
研究活動
研究分野
受賞
2022年11月 日本動物遺伝育種学会, 第23回日本動物遺伝育種学会優秀発表賞学会長特別賞, 岩手県黒毛和種における白斑とMITF遺伝子多型との関連
2021年09月 日本畜産学会, 2021年度日本畜産学会賞, 兵庫県黒毛和種集団における肉質関連遺伝子の同定
2021年03月 日本畜産学会, 第128回日本畜産学会大会優秀発表賞, 父母系および高密度SNPマーカーを用いたフィリピン在来ヤギの遺伝構造と伝播の推定
2020年11月 日本動物遺伝育種学会, 第21回日本動物遺伝育種学会優秀発表賞学会長特別賞, ネパール在来ヤギにおけるEPAS1, FGF5遺伝子多型と高地適応形質との関連調査
2019年11月 日本動物遺伝育種学会, 第20回日本動物遺伝育種学会優秀発表賞, ミトゲノム配列によるハプログループT4の系統解析
2018年09月 日本動物遺伝育種学会, 第19回日本動物遺伝育種学会優秀発表賞学会長特別賞, カザフスタン在来ヤギに対するmtDNAとY染色体の塩基配列解析～Eurasian Steppeは重要なヤギの伝播経路の一つである～
日本国国内学会・会議・シンポジウム等の賞
2018年09月 日本動物遺伝育種学会, 第19回日本動物遺伝育種学会優秀発表賞, 兵庫県黒毛和種における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出
日本国国内学会・会議・シンポジウム等の賞
2018年03月 日本畜産学会, 第124回日本畜産学会大会優秀発表賞, ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
日本国国内学会・会議・シンポジウム等の賞
2017年09月 関西畜産学会, 第67回関西畜産学会優秀発表賞2, Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析
日本国国内学会・会議・シンポジウム等の賞
2017年09月 関西畜産学会, 第67回関西畜産学会優秀発表賞1, 北海道ホルスタインは希少mtDNAハプログループPを有する
日本国国内学会・会議・シンポジウム等の賞
2017年03月 Japanese Society of Animal Science, Animal Science Journal Excellent Paper award, Genome Wide association study for fatty acid composition in Japanese Black cattle
日本国国内学会・会議・シンポジウム等の賞
2016年11月 日本動物遺伝育種学会, 第17回日本動物遺伝育種学会大会・学会長特別賞, 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連
日本国国内学会・会議・シンポジウム等の賞
2015年03月 Japanese Society of Animal Science, Animal Science Journal Excellent Paper award, Effect of DNA polymorphisms related to fatty acid composition in adipose tissue
日本国国内学会・会議・シンポジウム等の賞
2014年11月 日本動物遺伝育種学会, 第15回日本動物遺伝育種学会学会長特別賞, DigiTag2 assay を用いたアジア牛集団に対する遺伝的類縁関係と遺伝的構造の解析
国内学会・会議・シンポジウム等の賞
2013年03月 Japanese Society of Animal Science, Animal Science Journal Excellent paper award, Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds
国内学会・会議・シンポジウム等の賞
2011年03月 日本畜産学会, 日本畜産学会奨励賞, 牛肉の品種及び産地を識別するDNAマーカーの開発
国内学会・会議・シンポジウム等の賞
論文
- Wiley, 2022年10月, Animal Science Journal, 93 (1), e13770, 英語
[査読有り]
研究論文（学術雑誌）
Japanese Black cattle (Japanese Wagyu) beef is attracting attention for its aroma and marbling, and its handling is increasing worldwide. Here, we focused on the origin discrimination of Wagyu beef and analyzed the nutritional components of Japanese Wagyu (produced in multiple prefectures of Japan), Hybrid Wagyu (a cross between Angus and Wagyu cattle born in Australia and transported to Japan), and Australian Wagyu beef using mass spectrometry (MS). Triple-quadrupole liquid chromatography-MS was used to clarify the molecular species of lipids in Wagyu beef. Fourteen classes of lipids were separated, and 128 different triacylglycerides (TGs) were detected. A simple comparative analysis of these TGs using high-performance liquid chromatography revealed significantly higher levels of triolein (C18:1/C18:1/C18:1; abbreviated OOO) and C18:1/C18:1/C16:1 (OOPo) in Japanese Wagyu. Wagyu elements beef were comprehensively analyzed using inductively coupled plasma (ICP)-MS and ICP-optical emission spectrometry. We found significant differences in the rubidium, cesium, and lithium levels of Japanese and Australian Wagyu beef. On comparing metabolites using gas chromatography-MS, we identified significant differences in the levels of amino acids and other components of the Japanese and Australian Wagyu beef. These results suggest the possibility of determining the origin of Wagyu cattle breeds using MS and genetic discrimination.
2022年08月, Metabolites, 12 (9), 777, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.
MDPI AG, 2022年07月01日, Genes, 13 (7), 1190 - 1190研究論文（学術雑誌）
- Wiley, 2022年06月25日, Molecular Ecology, 31 (16), 4364 - 4380
研究論文（学術雑誌）
Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the Bos genus were domesticated in Asia, where they also diversified. As native cattle (taurine cattle, zebu cattle, yak, and water buffalo) are indigenous and adapted to local environments, we hypothesized that Asian native cattle could have harbored BLV and, therefore, that they were important for virus emergence, maintenance, and spread. In this study, phylogeographic and ancestral trait analyses—including sequences obtained from Asian native cattle—were used to reconstruct the evolutionary history of BLV. It was shown that, since its probable emergence in Asia, the virus spread to South America and Europe via international trade of live cattle. It was inferred that zebu cattle were the hosts for the early origin of BLV, while taurine cattle played the significant role in the transmission worldwide. In addition, the results of positive selection analysis indicate that yak had a substantially minor role in the transmission of this virus. In this study, endogenous deltaretrovirus sequences in bats, collected in Asian countries, were also analyzed on whether these sequences were present in the bat genome. Endogenous deltaretrovirus sequences were detected from bat species endemic to specific regions and geographically isolated for a long time. Endogenous deltaretrovirus sequences from these geographically isolated species represent ancient exogenous deltaretroviruses distributions. The phylogenetic analysis revealed that these newly obtained endogenous deltaretrovirus sequences were closely related to those of BLV from Asian native cattle, indicating that BLV-related ancient deltaretroviruses circulated in Asia long before the emergence of BLV. Together, our analyses provide evidence for origin and spatiotemporal dynamics of BLV.
Frontiers Media SA, 2022年06月24日, Frontiers in Microbiology, 13研究論文（学術雑誌）
- 2021年12月, Animal Science Journal, 92 (1)Development of DNA markers for improvement of meat quality in a Japanese Black cattle population in Hyogo Prefecture
[査読有り][招待有り]
- Wiley, 2021年09月28日, Animal Science Journal, 92 (1)
[査読有り]
研究論文（学術雑誌）
- 2021年08月, The Journal of Animal Genetics, 49, 67 - 75, 英語Recent achievements of candidate polymorphism detection for fatty acid composition in Japanese Black cattle
[査読有り]
Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.
MDPI AG, 2021年06月22日, Life, 11 (7), 597 - 597[査読有り]
研究論文（学術雑誌）
Objective: Conservation and genetic improvement of cattle breeds require information about genetic diversity and population structure of the cattle. In this study, we investigated the genetic diversity and population structure of the three cattle breeds in the Korean peninsula.Methods: Jeju Black, Hanwoo, Holstein cattle in Korea, together with six foreign breeds were examined. Genetic diversity within the cattle breeds was analyzed with minor allele frequency (MAF), observed and expected heterozygosity (HO and HE), inbreeding coefficient (FIS) and past effective population size. Molecular variance and population structure between the nine breeds were analyzed using a model-based clustering method. Genetic distances between breeds were evaluated with Nei’s genetic distance and Weir and Cockerham’s FST.Results: Our results revealed that Jeju Black cattle had lowest level of heterozygosity (HE = 0.21) among the studied taurine breeds, and an average MAF of 0.16. The level of inbreeding was –0.076 for Jeju Black, while –0.018 to –0.118 for the other breeds. Principle component analysis and neighbor-joining tree showed a clear separation of Jeju Black cattle from other local (Hanwoo and Japanese cattle) and taurine/indicine cattle breeds in evolutionary process, and a distinct pattern of admixture of Jeju Black cattle having no clustering with other studied populations. The FST value between Jeju Black cattle and Hanwoo was 0.106, which was lowest across the pair of breeds ranging from 0.161 to 0.274, indicating some degree of genetic closeness of Jeju Black cattle with Hanwoo. The past effective population size of Jeju Black cattle was very small, i.e. 38 in 13 generation ago, whereas 209 for Hanwoo.Conclusion: This study indicates genetic uniqueness of Jeju Black cattle. However, a small effective population size of Jeju Black cattle indicates the requirement for an implementation of a sustainable breeding policy to increase the population for genetic improvement and future conservation.
Asian Australasian Association of Animal Production Societies, 2021年05月01日, Animal Bioscience, 34 (5), 789 - 800[査読有り]
研究論文（学術雑誌）
- 2020年11月, Animal Science JournalIndonesian native goats (Capra hircus) reveal highest genetic frequency of mitochondrial DNA haplogroup B in the world
[査読有り]
Abstract Surveys of mitochondrial DNA (mtDNA) variation have shown that worldwide domestic cattle are characterized by just a few major haplogroups. Two, T and I, are common and characterizeBos taurus andBos indicus , respectively, while the other three, P, Q and R, are rare and are found only in taurine breeds. Haplogroup P is typical of extinct European aurochs, while intriguingly modern P mtDNAs have only been found in northeast Asian cattle. These Asian P mtDNAs are extremely rare with the exception of the Japanese Shorthorn breed, where they reach a frequency of 45.9%. To shed light on the origin of this haplogroup in northeast Asian cattle, we completely sequenced 14 Japanese Shorthorn mitogenomes belonging to haplogroup P. Phylogenetic and Bayesian analyses revealed: (1) a post-glacial expansion of aurochs carrying haplogroup P from Europe to Asia; (2) that all Asian P mtDNAs belong to a single sub-haplogroup (P1a), so far never detected in either European or Asian aurochs remains, which was incorporated into domestic cattle of continental northeastern Asia possibly ~ 3700 years ago; and (3) that haplogroup P1a mtDNAs found in the Japanese Shorthorn breed probably reached Japan about 650 years ago from Mongolia/Russia, in agreement with historical evidence.Springer Science and Business Media LLC, 2020年11月, Scientific Reports, 10 (1)[査読有り]
研究論文（学術雑誌）
Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.
Copernicus GmbH, 2020年01月13日, Archives Animal Breeding, 63 (1), 9 - 17[査読有り]
研究論文（学術雑誌）
- Wiley, 2020年01月, Animal Science Journal, 91 (1), e13335
[査読有り]
研究論文（学術雑誌）
Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.
2019年12月, Heliyon, 5 (12), e03006, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- Zoological Society of Japan, 2019年08月01日, Zoological Science, 36 (4), 294 - 294, 英語
[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2019年06月, Meta Gene, 20, 100558 - 100558
[査読有り]
研究論文（学術雑誌）
- Wiley, 2019年04月, Animal Science Journal, 90 (4), 467 - 472, 英語
[査読有り]
研究論文（学術雑誌）
- (公社)日本畜産学会, 2019年03月, 日本畜産学会大会講演要旨集, 125回, 177 - 177, 日本語複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
- Wiley, 2019年03月, Animal Science Journal, 90 (3), 317 - 322, 英語
[査読有り]
研究論文（学術雑誌）
- Japanese Society of Animal Breeding and Genetics, 2019年, The Journal of Animal Genetics, 47 (2), 37 - 45
[査読有り]
研究論文（学術雑誌）
- Wiley, 2019年01月, Animal Science Journal, 90 (1), 29 - 34, 英語
[査読有り]
研究論文（学術雑誌）
- 2018年08月, 動物遺伝育種研究, 46 (2), 49 - 55, 日本語日本ホルスタインにおいて観察された稀少なmtDNAハプログループP
[査読有り]
研究論文（学術雑誌）
- Wiley, 2018年08月, Animal Science Journal, 89 (8), 1060 - 1066, 英語
[査読有り]
研究論文（学術雑誌）
- 2018年08月, 日本畜産学会報, 89 (3), 313 - 321, 日本語Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析
[査読有り]
研究論文（学術雑誌）
- Wiley, 2018年05月, Animal Science Journal, 89 (5), 743 - 751, 英語
[査読有り]
研究論文（学術雑誌）
- (公社)日本畜産学会, 2018年03月, 日本畜産学会大会講演要旨集, 124回, 111 - 111, 日本語ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
- Japanese Society of Animal Breeding and Genetics, 2018年, The Journal of Animal Genetics, 46 (2), 49 - 55
[査読有り]
研究論文（学術雑誌）
Background Aurochs (Bos primigenius) were distributed throughout large parts of Eurasia and Northern Africa during the late Pleistocene and the early Holocene, and all modern cattle are derived from the aurochs. Although the mtDNA haplogroups of most modern cattle belong to haplogroups T and I, several additional haplogroups (P, Q, R, C and E) have been identified in modern cattle and aurochs. Haplogroup P was the most common haplogroup in European aurochs, but so far, it has been identified in only three of > 3,000 submitted haplotypes of modern Asian cattle. Methodology We sequenced the complete mtDNA D-loop region of 181 Japanese Shorthorn cattle and analyzed these together with representative bovine mtDNA sequences. The haplotype P of Japanese Shorthorn cattle was analyzed along with that of 36 previously published European aurochs and three modern Asian cattle sequences using the hypervariable 410 bp of the D-loop region. Conclusions We detected the mtDNA haplogroup P in Japanese Shorthorn cattle with an extremely high frequency (83/181). Phylogenetic networks revealed two main clusters, designated as Pa for haplogroup P in European aurochs and Pc in modern Asian cattle. We also report the genetic diversity of haplogroup P compared with the sequences of extinct aurochs. No shared haplotypes are observed between the European aurochs and the modern Asian cattle. This finding suggests the possibility of local and secondary introgression events of haplogroup P in northeast Asian cattle, and will contribute to a better understanding of its origin and genetic diversity.
Public Library of Science, 2018年01月01日, PLoS ONE, 13 (1), e0190937, 英語[査読有り]
研究論文（学術雑誌）
- Wiley, 2018年01月, Animal Science Journal, 89 (1), 257 - 258, 英語
[査読有り]
研究論文（学術雑誌）
- Genetics and Molecular Research, 2018年, Genetics and Molecular Research, 17 (3), 18056, 英語
[査読有り]
研究論文（学術雑誌）
- 2017年09月, Animal Veterinary Sciences, 5, 69 - 72, 英語
[査読有り]
研究論文（学術雑誌）
This study aims to estimate the mitochondrial genetic diversity and structure of Japanese Polled and Kuchinoshima feral cattle, which are maintained in small populations. We determined the mitochondrial DMA (mtDNA) displacement loop (D-loop) sequences for both cattle populations and analyzed these in conjunction with previously published data from Northeast Asian cattle populations. Our findings showed that Japanese native cattle have a predominant, Asian-specific mtDNA haplogroup T4 with high frequencies (0.43-0.81). This excluded Kuchinoshima cattle (32 animals), which had only one mtDNA haplotype belonging to the haplogroup T3. Japanese Polled showed relatively lower mtDNA diversity in the average sequence divergence (0.0020) than other Wagyu breeds (0.0036-0.0047). Japanese Polled have been maintained in a limited area of Yamaguchi, and the population size is now less than 200. Therefore, low mtDNA diversity in the Japanese Polled could be explained by the decreasing population size in the last three decades. We found low mtDNA diversity in both Japanese Polled and Kuchinoshima cattle. The genetic information obtained in this study will be useful for maintaining these populations and for understanding the origin of Japanese native cattle.
WILEY, 2017年05月, ANIMAL SCIENCE JOURNAL, 88 (5), 739 - 744, 英語[査読有り]
研究論文（学術雑誌）
Previous studies have indicated that some leptin gene polymorphisms were associated with economically important traits in cattle breeds. However, polymorphisms in the leptin gene have not been reported thus far in Japanese Black cattle. Here, we aimed to identify the leptin gene polymorphisms which are associated with carcass traits and fatty acid composition in Japanese Black cattle. We sequenced the full-length coding sequence of leptin gene for eight Japanese Black cattle. Sequence comparison revealed eight single nucleotide polymorphisms (SNPs). Three of these were predicted to cause amino acid substitutions: Y7F, R25C and A80V. Then, we genotyped these SNPs in two populations (JB1 with 560 animals and JB2 with 450 animals) and investigated the effects on the traits. Y7F in JB1 and A80V in JB2 were excluded from statistical analysis because the minor allele frequencies were low (<0.1). Association analysis revealed that Y7F had a significant effect on the dressed carcass weight in JB2; R25C had a significant effect on C18:0 and C14:1 in JB1 and JB2, respectively; and A80V had a significant effect on C16:0, C16:1, C18:1, monounsaturated fatty acid and saturated fatty acid in JB1. The results suggested that these SNPs could be used as an effective marker for the improvement of Japanese Black cattle.
WILEY, 2017年03月, ANIMAL SCIENCE JOURNAL, 88 (3), 433 - 438, 英語[査読有り]
研究論文（学術雑誌）
- Japanese Society of Animal Breeding and Genetics, 2017年, 動物遺伝育種研究, 45 (1), 3 - 8, 日本語
[査読有り]
研究論文（学術雑誌）
本研究では口之島野生化牛，黒毛和種，土佐褐毛和種，ホルスタインの4集団を実験に供し，高密度SNPアレイを用いた包括的な遺伝的多様性解析を行った．MAFの平均値では，口之島野生化牛が0.089であり，他の3品種の平均値（0.212）と比較して半分以下の値を示し，口之島野生化牛が極めて低い遺伝的多様性を示した．染色体ごとの遺伝的多様性を調査した結果，単型を示すSNPの割合は染色体ごとに大きく異なり，最小値で24番染色体の47.7%，最大値で20番染色体の72.3%であった．さらに各染色体内での遺伝的多様性の変化を調査したところ，口之島野生化牛は他の3品種には見られない，単型を示すSNPが5 Mb以上にわたって連続する染色体領域が6ヵ所観察され，最大12.80Mbにも及んだ．以上の結果は，口之島野生化牛集団の成立過程における創始者効果と遺伝的浮動の影響が原因していると考えられた．
公益社団法人 日本畜産学会, 2016年08月, 日本畜産学会報, 87 (3), 219 - 226, 日本語[査読有り]
研究論文（学術雑誌）
Marker-assisted selection (MAS) is expected to accelerate the genetic improvement of Japanese Black cattle. However, verification of the effects of the genes for MAS in different subpopulations is required prior to the application of MAS. In this study, we investigated the allelic frequencies and genotypic effects for carcass traits of six genes, which can be used in MAS, in eight local subpopulations. These genes are SCD, FASN and SREBP1, which are associated with the fatty acid composition of meat, and NCAPG, MC1R and F11, which are associated with carcass weight, coat color and blood coagulation abnormality, respectively. The frequencies of desirable alleles of SCD and FASN were relatively high and that of NCAPG was relatively low, and NCAPG was significantly associated with several carcass traits, including carcass weight. The proportions of genotypic variance explained by NCAPG to phenotypic variance were 4.83 for carcass weight. We thus confirmed that NCAPG is a useful marker for selection of carcass traits in these subpopulations. In addition, we found that the desirable alleles of six genes showed no negative effects on carcass traits. Therefore, selection using these genes to improve target traits should not have negative impacts on carcass traits.
WILEY, 2016年04月, ANIMAL SCIENCE JOURNAL, 87 (4), 469 - 476, 英語[査読有り]
研究論文（学術雑誌）
In this study, we genotyped 117 autosomal single nucleotide polymorphisms using a DigiTag2 assay to assess the genetic diversity, structure and relationships of 16 Eurasian cattle populations, including nine cattle breeds and seven native cattle. Phylogenetic and principal component analyses showed that Bos taurus and Bos indicus populations were clearly distinguished, whereas Japanese Shorthorn and Japanese Polled clustered with European populations. Furthermore, STRUCTURE analysis demonstrated the distinct separation between Bos taurus and Bos indicus (K=2), and between European and Asian populations (K=3). In addition, Japanese Holstein exhibited an admixture pattern with Asian and European cattle (K=3-5). Mongolian (K=13-16) and Japanese Black (K=14-16) populations exhibited admixture patterns with different ancestries. Bos indicus populations exhibited a uniform genetic structure at K=2-11, thereby suggesting that there are close genetic relationships among Bos indicus populations. However, the Bhutan and Bangladesh populations formed a cluster distinct from the other Bos indicus populations at K=12-16. In conclusion, our study could sufficiently explain the genetic construction of Asian cattle populations, including: (i) the close genetic relationships among Bos indicus populations; (ii) the genetic influences of European breeds on Japanese breeds; (iii) the genetic admixture in Japanese Holstein, Mongolian and Japanese Black cattle; and (iv) the genetic subpopulations in Southeast Asia.
WILEY-BLACKWELL, 2016年02月, ANIMAL SCIENCE JOURNAL, 87 (2), 190 - 196, 英語[査読有り]
研究論文（学術雑誌）
- Japanese Society of Animal Science, 2016年, 日本畜産学会報, 87 (4), 333 - 338, 日本語
[査読有り]
研究論文（学術雑誌）
The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.
WILEY-BLACKWELL, 2015年08月, ANIMAL SCIENCE JOURNAL, 86 (8), 737 - 746, 英語[査読有り]
研究論文（学術雑誌）
This study was a first analysis of paternal genetic diversity for extensive Asian domestic goats using SRY gene sequences. Sequencing comparison of the SRY 3-untranslated region among 210 Asian goats revealed four haplotypes (Y1A, Y1B, Y2A and Y2B) derived from four variable sites including a novel substitution detected in this study. In Asian goats, the predominant haplotype was Y1A (62%) and second most common was Y2B (30%). Interestingly, the Y2B was a unique East Asian Y chromosomal variant, which differentiates eastern and western Eurasian goats. The SRY geographic distribution in Myanmar and Cambodia indicated predominant the haplotype Y1A in plains areas and a high frequency of Y2B in mountain areas. The results suggest recent genetic infiltration of modern breeds into South-East Asian goats and an ancestral SRY Y2B haplotype in Asian native goats.
WILEY-BLACKWELL, 2015年06月, ANIMAL GENETICS, 46 (3), 337 - 339, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition of beef adipose tissue is one of its important traits because a high proportion of monounsaturated fatty acid is related to favorable beef flavor and tenderness. In this study, we searched polymorphisms in full length coding DNA sequence of urotensin 2 recepter and investigated the effects on fatty acid composition (C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, monounsaturated fatty acid, saturated fatty acid). Eight single nucleotide polymorphisms (SNP) were identified by sequence comparison among eight animals, including five Japanese Black and three Holstein cattle. One of these SNP (c.866C>T) was predicted to cause amino acid substitutions (P289L) and the other seven synonymous SNP, including c.267C>T, were presumed to be in linkage disequilibrium. Therefore we selected two SNP (c.267C>T and c.866C>T) for further analysis. We investigated associations between these genotypes and fatty acid composition in three Japanese Black populations (n=560, 245 and 287) and a Holstein population (n=202). Tukey-Kramer's honestly significant difference test revealed that CC genotype in c.267C>T indicated lower C14:0 and higher C18:1 than the other genotypes in Japanese Black cattle and CC genotype in c.866C>T showed lower C16:1 than CT genotype in Holstein cattle (P<0.05). These results suggested that these genotypes would contribute to production of high-grade meat as selection markers in beef cattle.
WILEY-BLACKWELL, 2014年05月, ANIMAL SCIENCE JOURNAL, 85 (5), 499 - 505, 英語[査読有り]
研究論文（学術雑誌）
Genetic factors are known to influence meat quality in beef cattle. Expression of fatty acid desaturase 2 (FADS2) and fatty acid-binding protein 4 (FABP4) genes in the fat metabolism pathway has been reported to influence fat-related traits. In this study, we aimed to discover DNA polymorphisms in the promoter regions of the FADS2 and FABP4 genes in cattle populations to develop genetic markers. As a consequence, one single nucleotide polymorphism (SNP) in the FADS2 promoter (FADS2 g.-823G > A) and two linked SNPs in the FABP4 promoter (FABP4 g.-295A > G and FABP4 g.-287A > G) were identified. All of them were assumed to influence binding sites for transcription factors, suggesting these SNPs influence the genes' expression and beef quality. Therefore, we performed association analysis between the genotypes and traits. Our results revealed that the FADS2 g.-823G > A had significant effects on several traits including beef marbling score (BMS). BMS of the G/G animals in a Holstein population (2.36) was higher than that of the G/A animals (2.13). The same tendency was observed in Japanese Black populations. On the other hand, although the FABP4 SNPs also had significant effects on some traits, they did not show additive effects on any of the traits analyzed in this study. Our results suggest that the FADS2 g.-823G > A is a useful genetic marker for breeding in beef cattle. (C) 2014 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2014年05月, LIVESTOCK SCIENCE, 163, 34 - 40, 英語[査読有り]
研究論文（学術雑誌）
Pooled genomic DNA has been proposed as a cost-effective approach in genomewide association studies (GWAS). However, algorithms for genotype calling of biallelic SNP are not adequate with pooled DNA samples because they assume the presence of 2 fluorescent signals, 1 for each allele, and operate under the expectation that at most 2 copies of the variant allele can be found for any given SNP and DNA sample. We adapt analytical methodology from 2-channel gene expression microarray technology to SNP genotyping of pooled DNA samples. Using 5 datasets from beef cattle and broiler chicken of varying degrees of complexity in terms of design and phenotype, continuous and dichotomous, we show that both differential hybridization (M = green minus red intensity signal) and abundance (A = average of red and green intensities) provide useful information in the prediction of SNP allele frequencies. This is predominantly true when making inference about extreme SNP that are either nearly fixed or highly polymorphic. We propose the use of model-based clustering via mixtures of bivariate normal distributions as an optimal framework to capture the relationship between hybridization intensity and allele frequency from pooled DNA samples. The range of M and A values observed here are in agreement with those reported within the context of gene expression microarray and also with those from SNP array data within the context of analytical methodology for the identification of copy number variants. In particular, we confirm that highly polymorphic SNP yield a strong signal from both channels (red and green) while lowly or nonpolymorphic SNP yield a strong signal from 1 channel only. We further confirm that when the SNP allele frequencies are known, either because the individuals in the pools or from a closely related population are themselves genotyped, a multiple regression model with linear and quadratic components can be developed with high prediction accuracy. We conclude that when these approaches are applied to the estimation of allele frequencies, the resulting estimates allow for the development of cost-effective and reliable GWAS.
AMER SOC ANIMAL SCIENCE, 2014年05月, JOURNAL OF ANIMAL SCIENCE, 92 (5), 1874 - 1885, 英語[査読有り]
研究論文（学術雑誌）
A putative functional mutation (rs109231213) near PLAG1 (BTA14) associated with stature was studied in beef cattle. Data from 8199 Bos taurus, Bos indicus and Tropical Composite cattle were used to test the associations between rs109231213 and various phenotypes. Further, 23496 SNPs located on BTA14 were tested for association with these phenotypes, both independently and fitted together with rs109231213. The C allele of rs109231213 significantly increased hip height, weight, net food intake, age at puberty in males and females and decreased IGF-I concentration in blood and fat depth. When rs109231213 was fitted as a fixed effect in the model, there was an overall reduction in associations between other SNPs and these traits but some SNPs remained associated (P<10(-4)). Frequency of the mutant C allele of rs109231213 differed among B.indicus (0.52), B.taurus (0.96) and Tropical Composite (0.68). Most chromosomes carrying the C allele had the same surrounding 10 SNP haplotype, probably because the C allele was introgressed into Brahman from B.taurus cattle. A region of reduced heterozygosity surrounds the C allele; this is small in B.taurus but 20Mb long in Brahmans, indicating recent and strong selection for the mutant allele. Thus, the C allele appears to mark a mutation that has been selected almost to fixation in the B.taurus breeds studied here and introduced into Brahman cattle during grading up and selected to a frequency of 0.52 despite its negative effects on fertility.
WILEY-BLACKWELL, 2013年12月, ANIMAL GENETICS, 44 (6), 636 - 647, 英語[査読有り]
研究論文（学術雑誌）
- 兵庫県黒毛和種肥育集団における経済形質関連遺伝子の効果
本研究では、兵庫県黒毛和種肥育集団の個体539頭を用い、SCD、SREBP-1、FASN、EDG-1、F11、MC1R、CW-2の遺伝子頻度を求め、脂肪酸組成および枝肉格付形質に対する遺伝子効果を調査した。優良アリル頻度はSCDで0.960、SREBP-1で0.322、FASNで0.910、EDG-1で0.265、F11で0.623、MC1Rで0.464、CW-2で0.007であった。SCD、SREBP-1、FASN、EDG-1では脂肪酸組成に、MC1Rでは枝肉重量に対し効果が認められた。SCDではAアリル、SREBP-1ではLアリルに不飽和脂肪酸を増加する効果が認められた。MC1RではED/ED遺伝子型において枝肉重量が大きい効果が認められた。経済形質関連遺伝子の遺伝子頻度や効果の大きさは集団ごとに異なるため、全国の黒毛和種の改良に大きく寄与した兵
日本畜産学会, 2013年11月, 日本畜産学会報, 84 (2), 157 - 162, 日本語[査読有り]
研究論文（学術雑誌）
- ミトコンドリアDNA を用いた東南アジア在来ヤギにおける遺伝的多様性解析
バングラデシュ在来ヤギ53個体とフィリピン在来ヤギ30個体におけるmtDNA D-loop HVI領域の塩基配列を決定し、さらにアジア在来ヤギの情報を加えA、Bハプログループの起源について考察を行った。バングラデシュとフィリピン在来ヤギではそれぞれ25および5ハプロタイプに分類され、AおよびBハプログループから構成されていた。バングラデシュとフィリピン在来ヤギのBハプログループにおける塩基置換率は、それぞれ0.0009と0.0006であり、極めて低い多様性を示した。また家畜化起源を推測するため、中東からの地理的距離と塩基置換率の相関を調べた。Aハプログループにおける順位相関係数はr =-0.7200であり有意な負の相関を示した（p=0.0469）。一方、Bハプログループにおいては有意な相関を得られなかった（p=0.7782）。この結果は、ヤギのAハプ
日本畜産学会, 2013年11月, 日本畜産学会報, 84 (2), 149 - 155, 日本語[査読有り]
研究論文（学術雑誌）
Fatty acid composition is one of the important traits in beef. The aim of this study was to identify candidate genomic regions for fatty acid composition by genome-wide association study with 50K single nucleotide polymorphism (SNP) array in Japanese Black cattle. A total of 461 individuals and 40657 SNPs were used in this study. We applied genome-wide rapid association using mixed model and regression (GRAMMAR) and genomic control approaches to estimate the associations between genotypes and fatty acid composition. In addition, two SNPs in fatty acid synthase (FASN) (T1952A) and stearoyl-CoA desaturase (SCD) (V293A) genes were also genotyped. Association analysis revealed that 30 significant SNPs for several fatty acids (C14:0, C14:1, C16:1 and C18:1) were located in the BTA19FASN gene located within this region but the FASN mutation had no significant effect on any traits. We also detected one significant SNP for C18:1 on BTA23 and two SNPs for C16:0 on BTA25. The region around 17Mb on BTA26 harbored two significant SNPs for C14:1 and SNP in SCD in this region showed the strongest association with C14:1. This study demonstrated novel candidate regions in BTA19, 23 and 25 for fatty acid composition.
WILEY-BLACKWELL, 2013年10月, ANIMAL SCIENCE JOURNAL, 84 (10), 675 - 682, 英語[査読有り]
研究論文（学術雑誌）
Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise F-ST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies.
WILEY-BLACKWELL, 2013年08月, ANIMAL SCIENCE JOURNAL, 84 (8), 585 - 591, 英語[査読有り]
研究論文（学術雑誌）
In our previous study, we examined divergently selected regions between Japanese Black cattle and Holstein cattle based on a 50k single nucleotide polymorphism (SNP) panel and a Sliding Window Allele Difference method resulting in the identification of 11 genomic regions. The aim of the present study was to investigate the association between these genomic regions and economic traits, including seven carcass and five milk production traits. For this purpose, representative SNP markers were selected from the 11 genomic regions and used to estimate the effects on the traits in Japanese Black cattle (N=488) and Holstein cattle (N=194). Association analyses revealed that five SNPs showed a significant effect on the carcass traits in Japanese Black cattle and other five SNP showed a significant association with milk production traits in Holstein cattle (P< 0.05). These results indicated that divergently selected regions identified using a Sliding Window Allele Difference method contain the quantitative trait loci (QTL) for economic traits. Furthermore, most of the QTL identified in this study were consistent with the previously reported QTL. Further investigations of these regions may lead to the identification of the genes and polymorphisms responsible for the economic traits. © 2013 Elsevier B.V.
Elsevier, 2013年08月, Livestock Science, 155 (2-3), 180 - 185, 英語[査読有り]
研究論文（学術雑誌）
In the current study, a total of 65 single nucleotide polymorphisms (SNPs) within the intron region were developed in goat (Capra hircus) by utilizing genomic information of cattle and sheep due to poor available genomic information on goat. Using these markers, we carried out genetic diversity and structure analyses for 10 Asian goat populations. The phylogenetic tree and principal components analysis showed good correspondence between clustered populations and their geographic locations. The STRUCTURE software analysis illustrated six divergent genetic structures among 10 populations. Myanmar and Cambodia populations showed high admixture patterns with different ancestry, suggesting genetic introgression into native goat populations. We also investigated the correlation between genetic diversity and geographic distance from a domestication center. This result showed a decreasing trend of genetic diversity according to the distance (P = 0.014). This result supported common consensus that western Asia is one of the centers of origin for modern Asian domestic goat.
WILEY-BLACKWELL, 2013年08月, ANIMAL SCIENCE JOURNAL, 84 (8), 579 - 584, 英語[査読有り]
研究論文（学術雑誌）
- 黒毛和種の背最長筋を用いた網羅的遺伝子発現解析
黒毛和種の遺伝的改良において、近年脂肪酸組成が食味性への影響から脚光を浴びている。本研究は脂肪酸組成に差のある集団間で発現量の異なる遺伝子を同定し、育種選抜を推進するために必要なDNA マーカーの候補とすることを目的とした。この目的のため、日本各地から集められた黒毛和種集団を筋肉内一価不飽和脂肪酸 (MUFA)含有率が高いグループと低いグループに分け、マイクロアレイによる網羅的遺伝子発現解析を行った。その結果、SPP1 (secreted phosphoprotein-1)、LEP (leptin)、MMP14 (matrix metallopeptidase 14)、IGF1 (insulin-like growth factor-1)、FGF1 (fibroblast growth factor-1)、FGF2 など脂肪酸代謝に関与すると考えられ
日本動物遺伝育種学会, 2013年06月, 動物遺伝育種研究, 41 (1), 7 - 14, 日本語[査読有り]
研究論文（学術雑誌）
Genetic analyses have contributed to improvements of economically important traits derived from adipose tissue such as fatty acid composition in beef. Elongation of very long chain fatty acids (ELOVL) genes encode for the enzymes that play an important role in elongation of long-chain fatty acids. In this study, we aimed to discover genetic polymorphisms of ELOVL gene family in cattle populations to develop genetic markers. As a result, five synonymous mutations were detected in the coding regions of the ELOVL1, ELOVL2, ELOVL3 and ELOVL5 genes. In addition, six mutations were identified in promoter region of the ELOVL5. Two of five mutations in the promoter region of ELOVL5 were expected to alter the ELOVL5 expression and influence the economic traits, because of the high synteny of the region which was essential for activation of Elovl5 in mouse. Therefore, we performed association analysis between the genotypes and traits and our result revealed that T allele of g.-110T > C in ELOVL5 gene promoter indicated significantly thinner subcutaneous fat thickness (TT, 2.39 cm; CT, 2.35 cm) than that of C allele (CC, 2.68 cm) in a Japanese Black population. Our results suggest that the g.-110T > C is a useful genetic marker for the breeding in beef cattle.
SPRINGER, 2013年04月, MOLECULAR BIOLOGY REPORTS, 40 (4), 3231 - 3237, 英語[査読有り]
研究論文（学術雑誌）
The domestic goat is one of the most important livestock species, but its origins and genetic diversity still remain uncertain. Multiple highly divergent maternal lineages of goat have been reported in previous studies. Although one of the mitochondrial DNA lineages, lineage B, was detected only in eastern and southern Asia, the geographic distribution of these lineages was previously unclear. Here, we examine the genetic diversity and phylogeographic structure of Asian goats by mitochondrial DNA sequences and morphological characteristics. The analyses of a total of 1661 Asian goats from 12 countries revealed a high frequency of lineage B in Southeast Asia. The frequency of this lineage tended to be higher in mountain areas than in plain areas in Southeast Asian countries, and there was a significant correlation between its frequency and morphological traits. The results suggest an original predominance of lineage B in Southeast Asia and the recent infiltration of lineage A into Southeast Asian goats.
WILEY-BLACKWELL, 2013年02月, ANIMAL GENETICS, 44 (1), 79 - 85, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition is an important economic trait for both dairy and beef cattle and controlled by genetic factors. Candidate genes controlling fatty acid composition may be found in fat synthesis and metabolism pathways. Acetyl-CoA carboxylase is the flux-determining enzyme in the regulation of fatty acid synthesis in animal tissues. One of two isozymes of this enzyme, acetyl-CoA carboxylase-alpha (ACACA), catalyses the first committed step of fatty acid synthesis in mammalian cytosol, leading to the biosynthesis of long-chain fatty acids. In the current study, the sequence comparison of the coding sequence (CDS) and two promoter regions (PIA and PIII) in bovine ACACA gene was performed between Japanese Black and Holstein cattle to detect nucleotide polymorphisms influencing fatty acid composition in milk and beef. Five single nucleotide polymorphisms (SNPs) were identified in the CDS region, 28 SNPs in the PIA region and three SNPs in the PIII region. Association study revealed that CCT/CCT type of PIII_#1, #2/PIA_#26 indicated a higher percentage of C14:0 in the milk of the Holstein cattle than CCT/GTC type (p = 0.050) and that a difference of the percentage of C16:0 was observed between CCT/CCT and GTC/GTC type (p = 0.023). CDS_#2 T/T type indicated a higher percentage of C18:0 than T/C type (p = 0.008). In addition, the Japanese Black cattle with CC/GT type of PIII_#1, #2 showed a higher percentage of C18:2 in the meat than those with GT/GT type (p = 0.025). Since PIII is the promoter specific to mammary gland during lactation, the altered expression of the ACACA gene owing to the SNPs in the PIII region may influence the fatty acid composition in the milk.
SPRINGER, 2012年09月, MOLECULAR BIOLOGY REPORTS, 39 (9), 8637 - 8644, 英語[査読有り]
研究論文（学術雑誌）
The estimated allele frequency at neutral DNA marker loci is a fundamental parameter for establishing a conservation scheme for a set of livestock breeds. In this study, we propose a novel minimum distance (MD) method for estimating neutral allele frequencies, which minimizes the error by the use of pedigree information. Using computer simulation and actual microsatellite data for a pedigreed cattle population, the performance of the proposed method was compared with that of conventional random sampling (RND). MD manifests a promising superiority to RND, irrespective of the sample size and the depth of pedigree. It is theoretically demonstrated that the error of the estimates depends on both the sample size and the distance of founder allele frequencies between the sampled and the non-sampled individuals. MD samples individuals so as to minimize the latter source of estimation error.
WILEY-BLACKWELL, 2012年06月, JOURNAL OF ANIMAL BREEDING AND GENETICS, 129 (3), 226 - 233, 英語[査読有り]
研究論文（学術雑誌）
FASN is a multifunctional enzyme that catalyzes de novo biosynthesis of long-chain saturated fatty acid, and alteration of this enzyme may affect economic traits including fatty acid composition. Sequencing the coding sequence (CDS) of the FASN gene has so far been done only partially for dairy cattle. In this study, the CDS of the FASN gene of eight Holstein cattle was sequenced to detect nucleotide polymorphisms. We could identify 13 single nucleotide polymorphisms (SNPs) of which five were non-synonymous mutations (H1390Y, I1856L, T1950A, W1955R and T2264A). The association between these SNPs and economic traits was analyzed using 198 Holstein dams. Genotypes of H1390Y corresponded to those of the I1856L in the group, suggesting these SNPs were linked. Furthermore, the T1950A was suggestively linked with the W1955R. The association study revealed that T1950A/W1955R had an effect on milk fat content and the C14 Index. This will help to understand the complex association between the FASN gene and economic traits. (C) 2011 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2012年04月, LIVESTOCK SCIENCE, 144 (3), 281 - 284, 英語[査読有り]
研究論文（学術雑誌）
Differences between average allelic frequencies of genes that relate to traits suggest that it would be evidence of artificial selections. Sliding window approach is a useful method to identify genomic regions that have been differently selected between two breeds. The objective of this study was to identify the divergently selected regions between Japanese Black (JB) and Japanese Holstein (JH) cattle based on genotypic information obtained through a high-density single nucleotide polymorphism (SNP) panel. After genotyping of 54 001 SNP markers on 100 animals (50 JB and 50 JH), 40 635 SNPs were suitable for the analysis. For each of these SNPs, the absolute difference between allelic frequencies of JB and JH was calculated. In the current study, 10 consecutive SNPs were defined as components of a window. For each window, the average difference in allelic frequency was calculated. This was termed sliding window average difference (SWAD). Among 40 055 windows, we focused on 39 windows with the largest SWAD. This was equivalent to 0.1% of all windows and the SWAD was more than 0.435. Some of these windows overlapped and were distributed in 11 regions. These regions were in good agreement with reported quantitative trait locus, therefore would be selection signatures and good candidates that harbor the causative mutations.
WILEY-BLACKWELL, 2012年, ANIMAL SCIENCE JOURNAL, 83 (1), 7 - 13, 英語[査読有り]
研究論文（学術雑誌）
- 日本畜産学会, 2011年11月, 日本畜産学会報, 82 (2), 125 - 130, 日本語黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果
[査読有り]
研究論文（学術雑誌）
In the meat industry, correct labeling of beef origins or breed is required to assure quality and safety. This paper describes the development of discrimination markers between Japanese domestic and imported beef from the United States (US) and Australia (AUS) based on a bovine 50K single nucleotide polymorphism (SNP) array using a total of 110 samples: Japanese Black (n = 50), Japanese Holstein (n = 50) and US cattle (n = 10). Genotyping information revealed 1081 SNPs as candidate markers that were polymorphic only in US cattle. The genotyping results by PCR - restriction length polymorphism in Japanese Black (n = 300) and Holstein cattle (n = 146) revealed that 11 SNPs had alleles specific to US cattle. Their allelic frequencies in US cattle (n = 108) ranged from 0.097 to 0.250 with an average of 0.178 and the combined identification probability of US cattle was 0.987. In addition, we also verified the applicability of these US-specific markers to AUS cattle. Their allelic frequencies in AUS cattle (n = 280) ranged from 0.063 to 0.224 with an average of 0.137 and the combined identification probability of AUS cattle was 0.963. In conclusion, a set of these markers could be useful for discriminating between Japanese domestic and imported beef and would contribute to identify origins and prevent falsified labeling of beef.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (1), 67 - 72, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition of adipose tissue has been recognized as an important carcass trait because of its relationship with eating quality such as favorable beef flavor and tenderness. Therefore, we investigated the effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) on fatty acid composition in intramuscular fat tissue of Holstein steers. The major allele frequencies were 0.705 in SCD, 0.518 in FABP4, 0.888 in FASN, and 0.984 in LXR. Genotyping of SCD showed significant effect on C14:0, C14:1, C18:0 and saturated fatty acid (P < 0.05). In addition, the result suggested that SCD genotype possibly had effect on composition of C18:1 and monounsaturated fatty acid. Genotype of FABP4 had significant effect on composition of C16:0. Effect of LXR genotypes could not be analyze because of extremely biased genotype frequencies. Our results suggest that genotypes of SCD and FABP4 may in part affect meat quality in Holstein.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (3), 406 - 411, 英語[査読有り]
研究論文（学術雑誌）
Allele frequencies of 10 representative polymorphisms for beef and milk traits were investigated for a total of 240 animals from Bos taurus and Bos indicus breeds, including two Japanese groups (Japanese Black and Japanese Brown), two East Asian groups (Korean and Mongolian), three European groups (Holstein, Angus and Hereford) and a Bos indicus group in South Asia (Myanmar, Laos and Cambodia). The Japanese Black revealed unique genetic construction in GH, FASN and SREBP-1 and the other Asian populations show intermediate frequencies between European and Japanese populations. The Bos indicus group showed low favorable allele frequencies in most of the genes. The study showed the variability and distribution of 10 genes affecting economic traits among world representative cattle breeds. The genetic information would contribute to elucidating the genetic background for worldwide cattle breeds and the possibility of improvement using the markers.
WILEY-BLACKWELL, 2011年, ANIMAL SCIENCE JOURNAL, 82 (6), 717 - 721, 英語[査読有り]
研究論文（学術雑誌）
- Suppression of WWP1 Gene Via RNAi Induced the Reduction of Proliferation Rate of C2C12 Myoblasts
The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), which plays an important role in ubiquitin-proteasome pathway to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Previously, we transfected either of wild and mutated types of WWP1 gene into C2C12 mouse myoblasts to monitor the expression pattern of muscle-differentiation markers, so that excessive WWP1 expression enhanced the expression of the myosin heavy chain (MyHC) Ia gene but lowered the expression of the MyHC IIb gene, while mutated WWP1 gene transfected in to myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. However, the mechanism for the mutation to inhibit muscle differentiation remains elucidated. The current study attempted to suppress the WWP1 expression by RNAi technique and to observe its effect on C2C12 cells. The effect of WWP1 suppression was clearly different from that of the R441Q missense mutation in the WWP1 gene. The WWP1 suppression reduced the proliferation rate of C2C12 myoblasts, while clear difference was not observed in the proliferation rate by the transfection of the mutated WWP1 gene into the cells. Our RT-PCR analysis indicated that the reduction of the WWP1 expression is the specific effect mediated by RNAi and that the reduction of proliferation rate observed in this study is largely attributed to the suppression of the WWP1 expression. These data indicated that the mutation responsible for chicken muscular dystrophy does not eliminate the enzymatic activity and provides some new function for the gene.
JAPAN POULTRY SCIENCE ASSOC, 2010年10月, JOURNAL OF POULTRY SCIENCE, 47 (4), 288 - 293, 英語[査読有り]
研究論文（学術雑誌）
Members of the caveolin family are the main component of caveolae, and caveolin-3 is a muscle-specific protein. Caveolin-3 deficiency induces a muscular dystrophic phenotype, while its overexpression is also harmful to muscle cells Increased caveolae were observed in chicken muscular dystrophy, however, the underlying mechanism causing the onset remains unclear Therefore, the current study analyzes the expression of caveolin-3 and other caveola-related proteins in dystrophic chickens. Western blotting and semi-quantitative RT-PCR analysis revealed that (1) caveolin-3 is highly expressed in the damaged muscle of dystrophic chickens (7 12-fold), (2) the amount of caveolin-3 protein is regulated in posttranslational modification, since no significant increase is observed at the mRNA level (1.09-fold), and (3) the expression pattern of other caveola-related proteins is similar to that of caveolin-3 These results suggest that the accumulation of caveolin-3 protein may be associated with the causative process of chicken muscular dystrophy. (C) 2010 Elsevier Inc. All rights reserved
ELSEVIER SCIENCE INC, 2010年09月, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 157 (1), 68 - 72, 英語[査読有り]
研究論文（学術雑誌）
- Japanese Society of Animal Genetics, 2010年06月, Journal of Animal Genetics, 38 (1), 77 - 81, 英語Mitochondrial DNA variation and genetic construction of indigenous cattle population in Bhutan
[査読有り]
研究論文（学術雑誌）
In Japan, Japanese Black and Holstein cattle are appreciated as popular sources of meat, and imported beef from Australia and the United States is also in demand in the meat industry. Since the BSE outbreak, the problem of false sales has arisen: imported beef has sometimes been mislabeled as domestic beef due to consumer concerns. A method is needed to correctly discriminate between Japanese and imported cattle for food safety. The objective of this study was to develop breed discrimination markers between Japanese and US cattle using a 50K SNP array. As a result, five US-specific markers (BISNP7, BISNP15, BISNP21, BISNP23, and BISNP26) were developed with allelic frequencies that ranged from 0.102 (BISNP15) to 0.250 (BISNP7) and averaged 0.184. The combined use of the five markers would permit discrimination between Japanese and US cattle with a probability of identification of 0.858. This result indicates the potential of the bovine 50K SNP array as a powerful tool for developing breed identification markers. These markers would contribute to the prevention of falsified beef displays in Japan. (C) 2010 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2010年06月, MEAT SCIENCE, 85 (2), 285 - 288, 英語[査読有り]
研究論文（学術雑誌）
The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), an enzyme to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite of intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Since it is generally known that the switching of myosin heavy chain (MyHC) isoforms from neonatal isoform to adult one is inhibited in chicken muscular dystrophy, we transfected either of wild and mutated types of WWP1 gene into C2C12 cells to monitor the expression pattern of muscle-differentiation markers including MyHCs by real-time PCR. Excessive WWP1 expression enhanced the expression of the MyHC la gene but lowered the expression of the MyHC IIb gene. On the other hand, mutated WWP1 gene transfected into myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. The present data suggest that WWP1 promotes myoblast differentiation from embryonic into fast twitch phase while a mutation in WWP1 results to retain slow and fast twitch isoforms characteristic of dystrophic fast twitch muscles.
JAPAN POULTRY SCIENCE ASSOC, 2010年04月, JOURNAL OF POULTRY SCIENCE, 47 (2), 115 - 119, 英語[査読有り]
研究論文（学術雑誌）
Animal domestication was a major step forward in human prehistory, contributing to the emergence of more complex societies. At the time of the Neolithic transition, zebu cattle (Bos indicus) were probably the most abundant and important domestic livestock species in Southern Asia. Although archaeological evidence points toward the domestication of zebu cattle within the Indian subcontinent, the exact geographic origins and phylogenetic history of zebu cattle remains uncertain. Here, we report evidence from 844 zebu mitochondrial DNA (mtDNA) sequences surveyed from 19 Asiatic countries comprising 8 regional groups, which identify 2 distinct mitochondrial haplogroups, termed I1 and I2. The marked increase in nucleotide diversity (P < 0.001) for both the I1 and I2 haplogroups within the northern part of the Indian subcontinent is consistent with an origin for all domestic zebu in this area. For haplogroup I1, genetic diversity was highest within the Indus Valley among the three hypothesized domestication centers (Indus Valley, Ganges, and South India). These data support the Indus Valley as the most likely center of origin for the I1 haplogroup and a primary center of zebu domestication. However, for the I2 haplogroup, a complex pattern of diversity is detected, preventing the unambiguous pinpointing of the exact place of origin for this zebu maternal lineage. Our findings are discussed with respect to the archaeological record for zebu domestication within the Indian subcontinent.
OXFORD UNIV PRESS, 2010年01月, MOLECULAR BIOLOGY AND EVOLUTION, 27 (1), 1 - 6, 英語[査読有り]
研究論文（学術雑誌）
Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.
WILEY-BLACKWELL PUBLISHING, INC, 2010年, ANIMAL SCIENCE JOURNAL, 81 (2), 145 - 151, 英語[査読有り]
研究論文（学術雑誌）
This study describes the development of efficient single nucleotide polymorphism (SNP) markers for individual identification and parentage tests in a Japanese Black cattle population. An amplified fragment length polymorphism method was employed to detect informative candidate markers, and yielded 44 SNP markers from 220 primer combinations. 29 unlinked SNPs were finally selected as diagnostic markers. The allelic frequencies for each marker were estimated by using PCR-RFLP in the Japanese Black population. Based on the frequency data, the estimated identity power of these markers was 2.73 x 10-12. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.96929 and 0.99693, respectively. This panel of SNP markers is theoretically sufficient for individual identification, and would also be a powerful tool for a parentage test in Japanese Black cattle. The markers could contribute to the management of the beef industry in Japan.
WILEY-BLACKWELL PUBLISHING, INC, 2010年, ANIMAL SCIENCE JOURNAL, 81 (2), 152 - 157, 英語[査読有り]
研究論文（学術雑誌）
The purpose of this study was to assess genetic diversity, phylogenetic relationship and population structure among nine Eurasian cattle populations using 58 single nucleotide polymorphism (SNP) markers. The calculated distribution of minor allele frequencies and heterozygosities suggested that the genetic diversity of Bos indicus populations was lower than that of Bos taurus populations. Phylogenetic analyses revealed the main divergence between the Bos taurus and Bos indicus populations, and subsequently between Asian and European populations. By principal components analysis, the Bos taurus and Bos indicus populations were clearly distinguished with PC1 (61.1%); however, six Bos taurus populations clustered loosely and the partial separation between European and Asian groups was observed by PC2 (12.5%). The structure analysis was performed using the STRUCTURE program. Distinct separation between Bos taurus and Bos indicus was shown at K = 2, and that between European and Asian populations at K = 3. At K = 4, 5 and 6, Mongolian population showed an admixture pattern with different ancestry of Asian and European cattle. At K = 7, all Bos taurus populations showed each cluster with little proportion of admixture. In conclusion, 58 SNP markers in this study could sufficiently estimate the genetic diversity, relationship and structure for nine Eurasian cattle populations, especially by analyses of principal components and STRUCTURE.
WILEY-BLACKWELL, 2010年, ANIMAL SCIENCE JOURNAL, 81 (3), 281 - 289, 英語[査読有り]
研究論文（学術雑誌）
Individual identification and parentage analysis using DNA markers are essential for assuring food identity and managing livestock population. The objective of this study was to develop a single nucleotide polymorphism (SNP) panel system for individual effective identification and parentage testing in a Japanese Black cattle population using BovineSNP50 BeadChip. On the basis of SNP frequencies, 60 unlinked informative SNPs were finally selected as candidate markers. The allelic frequencies for each SNP were estimated using additional individuals by PCR-RFLP (restriction fragment length polymorphism). A total of 87 SNP markers added in conjunction with previously developed 27 SNPs were evaluated to assess the utility of the test. The estimated identity power was 2.01 x 10-34. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.99999997 and 0.99998010, respectively. This developed SNP panel was quite powerful and could successfully exclude false sires with a probability of > 0.9999 even if the dam's genotype information was not obtained. The SNP system would contribute to management of the beef industry in Japan.
WILEY-BLACKWELL, 2010年, ANIMAL SCIENCE JOURNAL, 81 (4), 506 - 512, 英語[査読有り]
研究論文（学術雑誌）
MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F2 Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.
Japanese Society of Animal Genetics, 2009年06月, Journal of Animal Genetics, 37 (1), 3 - 8, 英語[査読有り]
研究論文（学術雑誌）
MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F2 Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.
Japanese Society of Animal Genetics, 2009年06月, Journal of Animal Genetics, 37 (1), 3 - 8, 英語[査読有り]
研究論文（学術雑誌）
Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle.
WILEY-BLACKWELL PUBLISHING, INC, 2009年06月, ANIMAL SCIENCE JOURNAL, 80 (3), 225 - 232, 英語[査読有り]
研究論文（学術雑誌）
The WW domain containing E3 ubiquitin protein ligase 1 (WWP1) is classified into one of ubiquitin ligases which play an important role in ubiquitin-proteasome pathway. Previously, we identified the WWP1 gene as a candidate gene of chicken Muscular dystrophy by linkage analysis and sequence comparison. However, the mechanism causing pathological changes and underlaying gene function remains elucidated. In the present study. we analyzed the WWP1 gene expression in various Muscles and tissues of normal chickens, and compared with those Front muscular dystrophic chickens. Two mRNA isoforms were detected in all tissues examined and revealed almost equal expression level. The WWP1 expression of dystrophic chickens was decreased in almost all skeletal Muscles including unaffected Muscles. These data indicate that there might not be a causal relationship between the alteration of WWP1 expression level and the severity of muscular dystrophy.
JAPAN POULTRY SCIENCE ASSOC, 2009年04月, JOURNAL OF POULTRY SCIENCE, 46 (2), 95 - 99, 英語[査読有り]
研究論文（学術雑誌）
Growth hormone receptor (GHR) belongs to a member of the cytokine receptor superfamily. Polymorphism of presence or absence of an approximately 1.2 kbp LINE-1 element is observed in bovine GHR gene. The present study was carried out for estimating the genetic diversity and the origin of the LINE-1 element in 10 European, Southeastern Asian and East Asian cattle breeds or populations. Genotyping of the LINE-1 revealed predominant LINE-1 presence in European breeds (0.917 similar to 0.991), absence in the Bos taurus indicus populations (0.000 similar to 0.017), and intermediate presence in Northeast Asian cattle (0.417 similar to 0.522). From genetic features of LINE families, LINE-1 of GHR could be attributed to the same origin in both European and Asian cattle, and Asian LINE-1 may not be derived from recent introgression. This result suggested that LINE-1 in bovine GHR gene could have arisen in an ancestral population of Bos taurus taurus.
WILEY, 2009年, ANIMAL SCIENCE JOURNAL, 80 (5), 528 - 531, 英語[査読有り]
研究論文（学術雑誌）
Background: Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding protein (FABP4), liver X receptor alpha (LXR alpha), cytochrome b(5) (Cyt b(5)), long-chain acyl-CoA synthetase (ACSL) 1, ACSL4 and diacylglycerol acyltransferase 2 (DGAT2) and fatty acid composition. Results: Sequence comparisons revealed 14 single nucleotide polymorphisms in six genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXR alpha, were nonsynonymous substitutions. The associations between the genotypes and fatty acid compositions were analyzed by using 234 Japanese Black cattle. The genotypes of FABP4 I74V and LXR alpha V133I were significantly associated with palmitoleic acids (C16: 1, P = 0.0086) and linoleic acid (C18:2, P = 0.0121) content in intramuscular fat, respectively. Conclusion: Our findings suggest that the two polymorphisms of FABP4 I74V and LXR alpha V133I might be genetic factors in part associated with palmitoleic acid (FABP4 I74V) and linoleic acid (LXR alpha V133I) composition in intramuscular fat of Japanese Black cattle, respectively. Especially, FABP4 I74V had highly significant effect (P < 0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.
BIOMED CENTRAL LTD, 2008年12月, BMC GENETICS, 9, 84, 英語[査読有り]
研究論文（学術雑誌）
This study seeks to investigate the genetic variability of PRNP in Asian goats. We sequenced the PRNP coding region using a total of 193 samples from seven Asian countries (Japan, Laos, Vietnam. Bhutan, Mongolia. Myanmar and Cambodia). Sequence comparison revealed five previously reported polymorphisms in the PRNP coding region. Two of those polymorphisms (G126A and C414T) were silent mutations, and the other three (T304G, A428G and T718C) Caused amino acid changes (W102G, H143R and S240P). In the total of 193 animals, one amino acid mutation (T304G) exhibited low variability (minor allele frequency = 0.04), but the other four were high (0.31-0.36). In addition, allele frequencies of C414T and T718C exhibited remarkable differences among countries (p-values of 6.50E-17 and 5.49E-18). These results Suggest high genetic variability of PRNP among these countries and are useful information for estimating genetic diversity in Asian goats. (C) 2008 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2008年11月, SMALL RUMINANT RESEARCH, 80 (1-3), 101 - 103, 英語[査読有り]
研究論文（学術雑誌）
Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1 Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence comparison and gene expression analysis to elucidate the responsible gene. One missense mutation was detected in AM candidate genes, while no remarkable alteration of expression patterns was observed. The mutation was identified in WWP1, detected only in dystrophic chickens within several tetrapods. These results suggested WWP1 is responsible for chicken muscular dystrophy. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2008年06月, FEBS LETTERS, 582 (15), 2212 - 2218, 英語[査読有り]
研究論文（学術雑誌）
- Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genes
In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and a 528 bp fragment of ND5 gene in the bovine mtDNA were amplified by PCR and digested with three restriction enzymes, Msel, HpyCH(4)III and Tsp509I, respectively. The results showed that Bos taurus (T) type was the majority in Hanwoo by combining three markers (99.5%). However, 78.2% of Bos indicus (1) type was observed in the imported beef samples. These results indicated that three markers used in this study will be used as valuable markers for discriminating imported beef against Hanwoo.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2008年01月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 21 (1), 25 - 28, 英語[査読有り]
研究論文（学術雑誌）
In this study, we describe intraspecies variation in the alpha C connector region of the bovine fibrinogen A alpha gene. Sequencing and genotyping of six bovine breeds revealed 7 to 10 tandem repeats in the alpha C connector region. In addition, we observed length differences between B. indicus and B. taurus, with the B. indicus having longer fibrinogen alpha C connectors (10-repeat alleles) than B. taurus (7- and 9-repeats). The difference in tandem repeats may be related to the function of blood coagulation system.
TAYLOR & FRANCIS INC, 2008年, Animal Biotechnology, 19 (2), 122 - 126, 英語[査読有り]
研究論文（学術雑誌）
To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.
SPRINGER, 2007年12月, MAMMALIAN GENOME, 18 (12), 880 - 886, 英語[査読有り]
研究論文（学術雑誌）
Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of monounsaturated fatty acids (MUFAs). In cattle, the MUFAs are related to softness and flavor of meat. In order to investigate gene expression profile during bovine preadipocyte differentiation, we isolated stromal-vascular cells from perirenal adipose tissues of Japanese Black and Holstein steers. Gene expression level of adipocyte type fatty acid binding protein (FABP4), SCD, sterol regulatory element binding protein 1 (SREBP1) and CCAAT/enhancer binding protein alpha (C/EBP-alpha) were elucidated by real-time PCR assay. The levels of SCD mRNA expression were significantly increased to 10.8 and 6.3-fold in Japanese Black and Holstein, respectively, on day 1 of the culture. The difference in SCD expression between the two breeds may reflect differences in the fat development characteristics of the cattle breeds. Although transcription factors SREBP1 and C/EBP-alpha are supposed to regulate SCD expression, expression levels of the two factors were not completely consistent with that of SCD. (c) 2007 Elsevier Inc. All rights reserved.
ELSEVIER SCIENCE INC, 2007年11月, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 148 (3), 629 - 634, 英語[査読有り]
研究論文（学術雑誌）
Muscular dystrophies, a group of inherited diseases with the progressive weakness and degeneration of skeletal muscle, contain genetically variable diseases. Though chicken muscular dystrophy with abnormal muscle (AM) has long been known, the gene responsible has not yet been identified. In this study, a resource family for AM was established with 487 F2 individuals and 22 gene markers, including microsatellite and insertion-deletion markers, were developed. The haplotypes were analyzed with these markers for the candidate region of GGA2q described in a previous study. The candidate region was successfully narrowed down to approximately 1Mbp. The region included seven functional genes predicted as the most likely AM candidates.
BLACKWELL PUBLISHING, 2007年10月, ANIMAL SCIENCE JOURNAL, 78 (5), 476 - 483, 英語[査読有り]
研究論文（学術雑誌）
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese and Australian beef. Two Bos indicus-specific markers and MCIR marker were used as possible candidate markers. Amplified fragment length polymorphism method was employed to develop additional candidate markers. The 1564 primer combinations provided three markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. In these markers, the allele frequencies in cattle from both countries were investigated for discrimination ability using PCR-RFLP. The probability of identifying Australian beef was 0.933 and probability of misjudgment was 0.017 using six selected markers. These markers could be useful for discriminating between Japanese and Australian beef and would contribute to the prevention of falsified breed labeling of meat. (c) 2007 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2007年10月, MEAT SCIENCE, 77 (2), 161 - 166, 英語[査読有り]
研究論文（学術雑誌）
- 2007年, 動物遺伝育種学会誌, 35(1):5-10, 5 - 10, 英語Genetic diversity of Bhutanese Cattle analyzed by mitochondrial DNA variation
[査読有り]
研究論文（学術雑誌）
Differences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was to identify candidate proteins causing differences of the meat qualities between the two breeds. Using technique of two-dimensional gel electrophoresis, protein profiling was compared from samples of the longissimus dorsi muscle and subcutaneous adipose tissue. Five protein spots were observed with different expression levels between breeds. By using LC-MS/MS analysis and Mascot program, three of them were identified as ankyrin repeat protein 2, phosphoylated myosin light chain 2 and mimecan protein. Subsequently, we compared the DNA coding sequences of three proteins between breeds to find any nucleotide substitution. However, there was no notable mutation which could affect pI or molecular mass of the proteins. The identified proteins may be responsible for different characteristics of the meat qualities between Japanese Black and Holstein cattle.
Asian-Australasian Association of Animal Production Societies, 2007年, Asian-Australasian Journal of Animal Sciences, 20 (5), 638 - 644, 英語[査読有り]
研究論文（学術雑誌）
- Mitochondrial DNA variation and genetic relationships in Japanese and Korean cattle
The complete mtDNA D-loop regions of Japanese and Korean cattle were analyzed for their mtDNA variations and genetic relationships. Sequencing the 30 Higo substrain and 30 Tosa substrain of Japanese Brown, respectively 12 and 17 distinct Bos haplotypes were identified from 77 polymorphic nucleotide sites. In order to focus on the relationships among Japanese and Korean cattle, two types of phylogenetic tree were constructed using individual sequences; first, a neighbor-joining tree with all sequences and second, reduced median networks within each Japanese and Korean cattle group. The trees revealed that two major mtDNA haplotype groups, T3 and T4, were represented in Japanese and Korean cattle. The T4 haplogroup predominated in Japanese Black and Japanese Brown cattle (frequency of 43.3-66.7%), while the T3 haplogroup was predominant (83.3%) and T4 was represented only twice in the Korean cattle. The results suggested that the mitochondrial origins of Japanese Brown were Japanese ancient cattle as well as Japanese Black in despite of the considerable introgression of Korean and European cattle into Japanese Brown.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, 2006年10月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19 (10), 1394 - 1398, 英語[査読有り]
研究論文（学術雑誌）
- Breed discrimination using DNA markers derived from AFLP in Japanese beef cattle
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black x Holstein) breeds. The amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 1,754 primer combinations yielded eleven markers that were converted into single nucleotide polymorphism markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.9168 and probability of misjudgment was 0.0066 using four selected markers. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the prevention of falsified breed labeling of meat.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, 2006年08月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19 (8), 1106 - 1110, 英語[査読有り]
研究論文（学術雑誌）
In order to develop a comparative map between chicken and quail, we identified orthologous gene markers based on chicken genomic sequences and localized them on the Japanese quail Kobe-NIBS linkage map, which had previously been constructed with amplified fragment length polymorphisms. After sequencing the intronic regions of 168 genes located on chicken chromosomes 1-8, polymorphisms among Kobe-NIBS quail family parents were detected in 51 genes. These orthologous markers were mapped on eight Japanese quail linkage groups (JQG), and they allowed the comparison of JQG to chicken macrochromosomes. The locations of the genes and their orders were quite similar between the two species except within a previously reported inversion on quail chromosome 2. Therefore, we propose that the respective quail linkage groups are macrochromosomes and designated as quail chromosomes CJA 1-8.
BLACKWELL PUBLISHING, 2006年08月, ANIMAL GENETICS, 37 (4), 316 - 320, 英語[査読有り]
研究論文（学術雑誌）
- Mitochondrial DNA diversity of Korean native goats
Korean native goats have lived on the Korean peninsula for more than 2,000 Nears and are regarded as a valuable genetic resource for the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis and calculation of genetic diversities have been performed using mitochondrial DNA (mtDNA) sequence variations. A total of 19 Korean native goats were grouped into six haplotypes and the large majority of haplotypes were present in 13 animals, All mtDNA of these Korean goat; belonged to the mitochondrial (mt) lineage A and revealed remarkably small genetic distances within the Population when compared with other Asian goat populations, indicating less genetic variation in the Korean native goats. These results indicate high-inbred status of the Korean native goats and will influence breeding and conservation strategies adopted for this breed.
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2006年04月, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19 (4), 482 - 485, 英語[査読有り]
研究論文（学術雑誌）
In our previous study, a Kobe-NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA-AFLP fragments and localized these markers on the linkage map, Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament-deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.
BLACKWELL PUBLISHING, 2006年02月, ANIMAL SCIENCE JOURNAL, 77 (1), 42 - 46, 英語[査読有り]
研究論文（学術雑誌）
- 日本動物遺伝育種学会, 2006年, 動物遺伝育種研究, 34 (1), 41 - 45
The Japanese quail (Coturnix japonica) is a notably valuable egg and meat producer but has also been used as a laboratory animal. In the present study, we constructed a Japanese quail linkage map with 1735 polymorphic amplified fragment length polymorphisms markers, and nine chicken microsatellite (MS) markers, as well as sex and phenotypes of two genetic diseases: a muscular disorder (LWC) and neurofilament-deficient mutant (Quv). Linkage analysis revealed 578 independent loci. The resulting linkage map contained 44 multipoint linkage groups covering 2597.8 cM and an additional 218.2 cM was contained in 21 two-point linkage groups. The total map was 2816 cM in length with an average marker interval of 5.5 cM. The Quv locus was located on linkage group 5, but linkage was not found between the LWC locus and any of the markers. Comparative mapping with chicken using orthologous markers revealed chromosomal assignments of the quail linkage group 1 to chicken chromosome 2 (GGA2), 5 to GGA22, 2 to GGA5, 8 to GGA7, 27 to GGA11, 29 to GGA1 and 45 to GGA4. © 2005 International Society for Animal Genetics.
2005年06月, Animal Genetics, 36 (3), 227 - 231, 英語研究論文（学術雑誌）
In order to estimate the influence of the Extension (E) locus in cattle coat color, the melanocortin-1 receptor (MC1R) gene in Japanese Black, Japanese Brown and Korean (Hanwoo) cattle were sequenced. The sequences of the coding region revealed three alleles (ED, E+ and e), which were previously reported. Polymerase chain reaction-restriction fragment length polymorphism was performed to investigate the gene frequencies of the three breeds. Japanese Black was almost composed of ED and E+ individuals, ED = 0.481 and E+ = 0.514, and no homozygous e/e, therefore that is consistent with the hypothesis that ED and E+ induce black pigment synthesis. Allele frequencies between Japanese Brown and Hanwoo were obviously different however, recessive red e allele frequency was 0.038 for Japanese Brown and 0.948 for Hanwoo, even though both breeds have quite similar coat colors (ranging from yellowish brown to dark brown including a red coat color). This result suggested that other genes are also associated with a coat color of red and brown in cattle.
2005年04月, Animal Science Journal, 76 (2), 129 - 132, 英語研究論文（学術雑誌）
- Japan Poultry Science Association, 2005年, J. Poult. Sci., 42:263-271 (3), 263 - 271, 英語Development and Mapping of Microsatellite Markersderived from cDNA in Japanese Quail (Coturnixjaponica).
研究論文（学術雑誌）
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black × Holstein) breeds. Amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 500 primer combinations yielded six selected markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.882 and probability of misjudgment was 0.0198. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the elimination of falsified breed labeling of meat. © 2003 Elsevier Ltd. All rights reserved.
2004年06月, Meat Science, 67 (2), 275 - 280, 英語研究論文（学術雑誌）
Using amplified fragment length polymorphism (AFLP) fingerprinting, selective genotyping was performed to determine if this method was effective for selecting superior breeding stock. Forty-eight cows with extreme genetic merit for beef marbling score (BMS) were selected from a population of Japanese Black cattle (n = 4462), including 25 with the highest for predicted breeding value (PBV) and 23 with the lowest. Sixteen AFLP fragments were selected for further analysis based on fragment frequency differences between the high and low groups. A linear discriminant analysis using these AFLP fragments was applied in order to derive a discriminant function that classified the cows into high and low groups. Seven of the 16 fragments were included in the resulting function and the discriminant scores (general genetic values, GGV) of the 48 cows were calculated using the function. These cows were clearly separated into high and low groups by GGV with a correlation ratio of 0.91 (discriminative error of 2.1%). The same function was then applied to 121 additional cows that were randomly selected from the original population. A significant regression coefficient of GGV on BMS-PBV (R2 = 0.45) was obtained, which indicates that the GGV can be used as a selection criterion for BMS in this population. These results suggest that AFLP fingerprinting can be used for animal breeding without identifying the underlying genes affecting the trait of interest.
2004年02月, Animal Genetics, 35 (1), 40 - 43, 英語研究論文（学術雑誌）
- 2004年, Proc. 29th Int. Conf. on Anim. Genet., p.138, 英語Selective genotyping for marker assisted selection in Japanese Black cattle.
研究論文（国際会議プロシーディングス）
Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p< 0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.
Asian-Australasian Association of Animal Production Societies, 2004年, Asian-Australasian Journal of Animal Sciences, 17 (10), 1355 - 1359, 英語研究論文（学術雑誌）
- 2001年, Anim. Sci. J., 72, 172Phylogenic analysis of five beef cattle breeds using AFLP marker.
[査読有り]
MISC
- 2019年05月, 畜産技術, 768, 16 - 20兵庫県黒毛和種集団における枝肉の画像解析形質を対象としたゲノムワイド関連解析
[招待有り]
- 2019年, 日本畜産学会大会講演要旨, 125th複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
- 2018年, 日本畜産学会大会講演要旨, 124thmtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析
- 2018年, 日本畜産学会大会講演要旨, 124th兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討
- 2018年, 日本畜産学会大会講演要旨, 124thウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
- 2017年, 日本畜産学会大会講演要旨, 122ndmtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, 英語Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.
研究発表ペーパー・要旨（国際会議）
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, 英語
研究発表ペーパー・要旨（国際会議）
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 18 - 18, 英語
研究発表ペーパー・要旨（国際会議）
- AMER SOC ANIMAL SCIENCE, 2016年09月, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, 英語
研究発表ペーパー・要旨（国際会議）
- 岡山実験動物研究会, 2014年04月, 岡山実験動物研究会報, (30), 68 - 69, 日本語ニワトリ筋ジストロフィーに関する研究
- 日本動物遺伝育種学会, 2012年12月01日, 動物遺伝育種研究, 40 (2), 78 - 78, 日本語ウシFABP4遺伝子のプロモーター領域に対する多型探索
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 73, 日本語岐阜県黒毛和種集団におけるUTS2R遺伝子内多型の脂肪酸組成に対する効果
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 85, 日本語黒毛和種におけるLEP遺伝子内多型の脂肪酸組成に対する効果
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 72, 日本語黒毛和種におけるFASN遺伝子内多型の脂肪酸組成に対する効果
- 2012年12月01日, 動物遺伝育種研究, 40 (2), 71, 日本語黒毛和種の枝肉形質に対するSCD,SREBP1,FASNおよびNCAPG遺伝子多型の関連解析
- (公社)日本畜産学会, 2012年03月, 日本畜産学会大会講演要旨集, 115回, 118 - 118, 日本語プーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価
- 2011年12月01日, 動物遺伝育種研究, 39 (2), 142, 日本語兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造
- 2011年12月01日, 動物遺伝育種研究, 39 (2), 133, 日本語ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索
- 2011年12月01日, 動物遺伝育種研究, 39 (2), 135, 日本語黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析
- 2011年12月01日, 動物遺伝育種研究, 39 (2), 142, 日本語黒毛和種における各地域集団の遺伝的多様性と類縁関係の解析
- The Korean Society of Poultry Science, 2011年06月, Korean Journal of Poultry Science, 38 (2), 145 - 154, 英語Identification of the gene responsible for chicken muscular dystrophy
[査読有り][招待有り]
記事・総説・解説・論説等（学術雑誌）
- 関西実験動物研究会, 2010年12月, 関西実験動物研究会会報, 32, 1 - 12, 日本語ニワトリ筋ジストロフィー責任遺伝子の同定とモデル動物としての展開
[招待有り]
記事・総説・解説・論説等（学術雑誌）
- (公社)日本畜産学会, 2009年09月, 日本畜産学会大会講演要旨集, 111回, 41 - 41, 日本語ニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析
書籍等出版物
- 北隆館, 2020年01月アグリバイオー和牛の育種改良
- その他, 兵庫県農政環境部農林水産局畜産課, 2018年03月, 日本語兵庫の和牛 但馬牛
学術書
- その他, ㈱シーエムシー出版, 2011年05月, 日本語食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―「DNA鑑定を利用した牛肉偽装表示の防止」
学術書
- 共編者(共編著者), 日本分析化学 表示･期限分析技術研究懇談会編, 2010年11月, 日本語食品表示を裏づける分析技術 科学の目で偽装を見破る 「品種鑑別 牛」
学術書
講演・口頭発表等
- 30th Plant & Animal Genome Conference, 2023年01月16日, 英語Genome-Wide Association Study for the Percentage of Oleic Acid in Japanese Black Cattle Revealed the Candidate Region on BTA19
- 30th Plant & Animal Genome Conference, 2023年01月16日, 英語Verification of Candidate SNP Effects Reveals Two QTLs on BTA7 for Beef Marbling in Two Japanese Black Cattle Populations
- 日本動物遺伝育種学会第23回大会, 2022年11月16日岩手県黒毛和種における白斑とMITF遺伝子多型との関連
- 日本畜産学会第130回大会, 2022年09月16日兵庫県黒毛和種におけるゲノムワイド関連解析を用いたオレイン酸含有率QTLの探索
- 日本動物遺伝育種学会第22回大会, 2021年11月13日アジア在来ヤギにおける角の有無と既報の1番染色体無角関連多型との関連
- 日本動物遺伝育種学会第22回大会, 2021年11月13日父母系および核ゲノムSNPマーカーを用いたインドネシア在来ヤギの遺伝構造解析及び遺伝子流入の推定
- 日本畜産学会第129回大会, 2021年09月15日黒毛和種現場後代検定調査牛集団における全ゲノムリシーケンスデータを用いたオレイン酸含有率に関わる多型の探索
- 38th International Society for Animal Genetics Conference, 2021年07月Confirmation of quantitative trait locus location on BTA19 for the percentage of oleic acid in beef based on effects of 5 polymorphisms and linkage disequilibrium analysis in 2 Japanese Black cattle populations.
- 日本畜産学会第128回大会, 2021年03月28日父母系および高密度 SNPマーカーを用いたフィリ ピン在来ヤギの遺伝構造と伝播の推定
- 日本畜産学会第128回大会, 2021年03月28日50K SNPアレイを用いた和牛4品種に関する多様性および類縁関係の調査
- 第21回日本動物遺伝育種学会大会, 2020年11月21日ネパール在来ヤギにおけるEPAS1, FGF5遺伝子多型と高地適応形質との関連調査
- 日本動物遺伝育種学会第21回大会, 2020年11月21日集積流体回路を用いた兵庫県黒毛和種集団におけるロース脂肪割合候補多型の効果の検証
- 第20回日本動物遺伝育種学会大会, 2019年11月30日ミトゲノム配列による ハプログループT4の系統解析
- 37th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Validation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic Circuit
ポスター発表
- 37th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Phylogenetic analysis of Nepalese goats using mtDNA D-loop region and SRY gene sequences
ポスター発表
- 37th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Genetic diversity of Bos taurus and Bos indicus using developed indel markers
ポスター発表
- 37th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Genetic diversity and structure in 11 native Asian goat populations analyzed by high density SNP array
ポスター発表
- 37th International Society for Animal Genetics Conference, 2019年07月, 英語, Lleida, Spain, 国際会議Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations
ポスター発表
- The 2nd Joint Meeting of Veterinary Science in East Asia, 2019年04月, 英語, Seoul, Korea, 国際会議Molecular epidemiological study of bovine leukemia virus in Zebu cattle (Bos indicus)
口頭発表（一般）
- 第125回日本畜産学会大会, 2019年03月, 日本語, 日本畜産学会, 相模原, 国内会議複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
口頭発表（一般）
- 第125回日本畜産学会大会, 2019年03月, 日本語, 日本畜産学会, 相模原, 国内会議mtDNA D-loop領域とSRY遺伝子の塩基配列に基づいたネパール在来ヤギの遺伝的類縁関係
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2018年09月, 日本語, 京都, 国内会議兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2018年09月, 日本語, 京都, 国内会議ゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2018年09月, 日本語, 京都, 国内会議カザフスタン在来ヤギに対するmtDNAとY染色体の塩基配列解析～Eurasian Steppeは重要なヤギの伝播経路の一つである～
口頭発表（一般）
- PAG Asia 2018, 2018年05月, 英語, Scherago International, Seoul, Korea, 国際会議Effect of SLC27A6 gene K81M polymorphism on fat percentage in rib-eye area in Japanese Black cattle
ポスター発表
- PAG Asia 2018, 2018年05月, 英語, Scherago International, Seoul, Korea, 国際会議Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data
ポスター発表
- 第124回日本畜産学会大会, 2018年03月, 日本語, 日本畜産学会, 東京, 国内会議兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討
口頭発表（一般）
- 第124回日本畜産学会大会, 2018年03月, 日本語, 日本畜産学会, 東京, 国内会議ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
口頭発表（一般）
- 第124回日本畜産学会大会, 2018年03月, 日本語, 日本畜産学会, 東京, 国内会議mtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2017年11月, 日本語, 動物遺伝育種学会, 厚木, 国内会議兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果
口頭発表（一般）
- 第18回日本動物遺伝育種学会大会, 2017年11月, 日本語, 動物遺伝育種学会, 厚木, 国内会議複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討
口頭発表（一般）
- 第67回関西畜産学会大会, 2017年09月, 日本語, 関西畜産学会, 大阪, 国内会議北海道ホルスタインは希少mtDNAハプログループPを有する
口頭発表（一般）
- 第67回関西畜産学会大会, 2017年09月, 日本語, 関西畜産学会, 大阪, 国内会議ゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索
口頭発表（一般）
- 第67回関西畜産学会大会, 2017年09月, 日本語, 関西畜産学会, 大阪, 国内会議Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析
口頭発表（一般）
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Phylogenetic analysis of Kazakhstani goats using mtDNA HV1 and SRY gene sequences
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Genome-wide association study revealed a candidate region for beef marbling on BTA7 in Japanese Black cattle
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Genetic structure and relationships among 11 cattle populations using Indel markers
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Effects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows
ポスター発表
- 36th International Society for Animal Genetics Conference, 2017年07月, 英語, International Society for Animal Genetics, Dublin, Ireland, 国際会議Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle
ポスター発表
- 第122回日本畜産学会大会, 2017年03月, 日本語, 日本畜産学会, 神戸, 国内会議mtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較
口頭発表（一般）
- 第17回日本動物遺伝育種学会大会, 2016年11月, 日本語, 日本動物遺伝育種学会, 名古屋, 国内会議黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連
口頭発表（一般）
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle
ポスター発表
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black
ポスター発表
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Genome-wide association study identifies a QTL for fat percentage in rib-eye area on BTA10 in Japanese Black cattle
ポスター発表
- 35th International Society for Animal Genetics Conference, 2016年07月, 英語, International Society for Animal Genetics, Slat Lake City, USA, 国際会議Genetic diversity and origin of mtDNA haplogroup P observed in Japanese Shorthorn
ポスター発表
- 第121回日本畜産学会大会, 2016年03月, 日本語, 日本畜産学会, 東京, 国内会議日本短角種において観察される希少なmtDNAハプログループPの母系起源
口頭発表（一般）
- 第121回日本畜産学会大会, 2016年03月, 日本語, 日本畜産学会, 東京, 国内会議ウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析
ポスター発表
- 第16回日本動物遺伝育種学会大会, 2015年11月, 日本語, 神戸, 国内会議GWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索
ポスター発表
- 第118回日本畜産学会大会, 2015年03月, 日本語, 日本畜産学会, 宇都宮, 国内会議黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果
口頭発表（一般）
- 第118回日本畜産学会大会, 2015年03月, 日本語, 日本畜産学会, 宇都宮, 国内会議高密度SNPアレイによる口之島牛の遺伝的多様性解析
口頭発表（一般）
- 第15回日本動物遺伝育種学会大会, 2014年11月, 日本語, 日本動物遺伝育種学会, 和光, 国内会議ホルスタイン種におけるFASN-g.841 及びg.16024 多型の乳形質に対する効果
ポスター発表
- 第15回日本動物遺伝育種学会大会, 2014年11月, 日本語, 日本動物遺伝育種学会, 和光, 国内会議SRY 遺伝子とmtDNA 塩基配列を用いたシバヤギの遺伝的多様性解析
ポスター発表
- 第15回日本動物遺伝育種学会大会, 2014年11月, 日本語, 日本動物遺伝育種学会, 和光, 国内会議DigiTag2 assay を用いたアジア牛集団に対する遺伝的類縁関係と遺伝的構造の解析
ポスター発表
- 第15回日本動物遺伝育種学会大会, 2014年11月, 日本語, 日本動物遺伝育種学会, 和光, 国内会議50K SNP チップを用いた口之島野生化牛の遺伝的多様性に関する研究
ポスター発表
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議Paternal genetic structure of Asian goats using SRY gene
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議LPL gene polymorphisms are associated with fatty acid composition in Japanese Black
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議Genome wide association study revealed candidate regions for calving interval in Japanese black cattle
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議Genetic diversity and relationship among 16 Asian and European cattle populations using 121 autosomal SNPs genotypes by the DigiTag2 assay
口頭発表（一般）
- 34th International Conference on Animal Genetics, 2014年07月, 英語, ISAG, Xian, China, 国際会議A genome wide association study for beef marbling and fatty acid composition in Japanese Black cattle using pooled DNA
口頭発表（一般）
- 第14回日本動物遺伝育種学会大会, 2013年10月, 日本語, 日本動物遺伝育種学会, 東京, 国内会議黒毛和種におけるLEP遺伝子プロモーター領域に対する多型探索
ポスター発表
- 第14回日本動物遺伝育種学会大会, 2013年10月, 日本語, 日本動物遺伝育種学会, 東京, 国内会議mtDNA塩基配列を用いた口之島野生化牛、日本短角種、無角和種の遺伝的多様性解析
ポスター発表
- 第116回日本畜産学会大会, 2013年03月, 日本語, 日本畜産学会, 広島, 国内会議ウシ脂肪酸組成に対するFASN 遺伝子内多型の効果
口頭発表（一般）
- 第116回日本畜産学会大会, 2013年03月, 日本語, 日本畜産学会, 広島, 国内会議SRY遺伝子を用いたアジア在来家畜ヤギの多様性解析
口頭発表（一般）
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議黒毛和種の枝肉形質に対するSCD、SREBP1、FASN およびNCAPG 遺伝子多型の関連解析
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議黒毛和種におけるLEP 遺伝子内多型の脂肪酸組成に対する効果
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議黒毛和種におけるFASN 遺伝子内多型の脂肪酸組成に対する効果
ポスター発表
- 第6回家畜DNA西郷シンポジウム, 2012年10月, 日本語, 独立行政法人家畜改良センター, 国内会議牛肉中脂肪の脂肪酸組成改良に向けた新規遺伝子マーカーの探索及び効果の検証
公開講演，セミナー，チュートリアル，講習，講義等
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 日本動物遺伝育種学会, 仙台, 国内会議岐阜県黒毛和種集団におけるUTS2R 遺伝子内多型の脂肪酸組成に対する効果
ポスター発表
- 第13回日本動物遺伝育種学会大会, 2012年10月, 日本語, 仙台, 国内会議ウシFABP4 遺伝子のプロモーター領域に対する多型探索
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議The SNPs in the ACACA gene effective on fatty acid composition in Holstein milk
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議The SNP in the promoter region of the bovine ELOVL5 gene is effective on subcutaneous fat thickness
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議Mutations in the PLAG1 region are associated with height, weight, puberty, IGF1, and fat deposition in cattle
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議Identification of quantitative trait loci affecting economic traits based on divergently selected regions between Japanese Black and Holstein cattle
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議Genome wide association study for fatty acid composition in Japanese Black cattle
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議Genetic diversity and structure in goat analyzed by newly-developed SNPs
ポスター発表
- 33th International Conference on Animal Genetics, 2012年07月, 英語, International Society for Animal Genetics, Cairns, Australia, 国際会議Evaluation of genetic diversity in Japanese Black cattle population using SNP-MaP strategy
ポスター発表
- 第115回日本畜産学会大会, 2012年03月, 日本語, 名古屋, 国内会議プーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価
口頭発表（一般）
- 第12回日本動物遺伝育種学会大会, 2011年11月, 日本語, 日本動物遺伝育種学会, 広島, 国内会議兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造
ポスター発表
- 第12回日本動物遺伝育種学会大会, 2011年11月, 日本語, 日本動物遺伝育種学会, 広島, 国内会議黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析
ポスター発表
- 第12回日本動物遺伝育種学会大会, 2011年11月, 日本語, 日本動物遺伝育種学会, 広島, 国内会議黒毛和種およびホルスタインにおけるUTS2R遺伝子多型の脂肪酸組成に対する効果
ポスター発表
- 第12回日本動物遺伝育種学会大会, 2011年11月, 日本語, 日本動物遺伝育種学会, 広島, 国内会議ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索
ポスター発表
- 第61回関西畜産学会大会, 2011年09月, 日本語, 関西畜産学会, 岡山, 国内会議黒毛和種閉鎖集団における形質関連遺伝子頻度の経時的変化
口頭発表（一般）
- 第61回関西畜産学会大会, 2011年09月, 日本語, 関西畜産学会, 岡山, 国内会議ウシELOVL5とELOVL6遺伝子のプロモーター領域に対する多型探索
口頭発表（一般）
- 第61回関西畜産学会大会, 2011年09月, 日本語, 関西畜産学会, 岡山, 国内会議DigiTag2 アッセイを用いたウシ個体識別及び親子鑑定システムの確立
口頭発表（一般）
- 第11回日本動物遺伝育種学会大会, 2010年10月, 日本語, 日本動物遺伝育種学会, 白河, 国内会議黒毛和種集団におけるsliding window approachを利用した枝肉形質関連遺伝子座の探索
ポスター発表
- 第11回日本動物遺伝育種学会大会, 2010年10月, 日本語, 日本動物遺伝育種学会, 白河, 国内会議ウシELOVL遺伝子の塩基配列決定及び多型探索
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Polymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattle
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Identification of divergently selected regions between Japanese Black and Holstein cattle based on a bovine 50k SNP array
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Genetic diversity and structure in Bos taurus and Bos indicus populations analyzed by SNP markers
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Development of discrimination markers between Japanese domestic and imported beef based on a bovine 50K SNP array
ポスター発表
- 32th International Conference on Animal Genetics, 2010年07月, 英語, International Society for Animal Genetics, Edinburgh, UK, 国際会議Allele frequencies of gene polymorphisms related to economic traits in Bos taurus and Bos indicus cattle breeds
ポスター発表
- 第112回日本畜産学会大会, 2010年03月, 日本語, 日本畜産学会, 明治大学, 国内会議黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果
口頭発表（一般）
- 第112回日本畜産学会大会, 2010年03月, 日本語, 日本畜産学会, 明治大学, 国内会議バングラデシュにおける 在来家畜及び野生原種の遺伝資源学的研究
口頭発表（一般）
- 第112回日本畜産学会大会, 2010年03月, 日本語, 日本畜産学会, 明治大学, 国内会議SNPマーカーを用いたウシの遺伝的多様性と遺伝構造解析
口頭発表（一般）
- 9th World Congress of Genetics Applied to Livestock Production, 2010年, 英語, 国際会議Sampling method for estimating neutral allele frequency in a pedigreed population
ポスター発表
- 第10回日本動物遺伝育種学会大会, 2009年11月, 日本語, 日本動物遺伝育種学会, 群馬会館, 国内会議ホルスタイン種におけるFASN遺伝子の多型探索及び乳形質の効果
ポスター発表
- 第10回日本動物遺伝育種学会大会, 2009年11月, 日本語, 日本動物遺伝育種学会, 群馬会館, 国内会議Bovine 50k SNPs情報を利用したウシゲノム解析
ポスター発表
- 第111回日本畜産学会大会, 2009年09月, 日本語, 日本畜産学会, 琉球大学, 国内会議国産牛と米国産牛とのＤＮＡ鑑定マーカーの開発
口頭発表（一般）
- 第111回日本畜産学会大会, 2009年09月, 日本語, 日本畜産学会, 琉球大学, 国内会議ホルスタイン種における枝肉形質に関る遺伝子多型の効果
口頭発表（一般）
- 第111回日本畜産学会大会, 2009年09月, 日本語, 日本畜産学会, 琉球大学, 国内会議ニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析
口頭発表（一般）
- 第111回日本畜産学会大会, 2009年09月, 日本語, 日本畜産学会, 琉球大学, 国内会議ウシ疾病関連遺伝子、抗病性関連遺伝子の多様性解析
口頭発表（一般）
- 第110回日本畜産学会大会, 2009年03月, 日本語, 日本畜産学会, 日本大学, 国内会議黒毛和種の個体識別および親子鑑定を目的としたSNPsパネルの開発
口頭発表（一般）
- 第110回日本畜産学会大会, 2009年03月, 日本語, 日本畜産学会, 日本大学, 国内会議ウシ経済形質関連遺伝子の多様性解析
口頭発表（一般）
共同研究・競争的資金等の研究課題
- 科学研究費補助金／基盤研究(C), 2022年04月 - 2025年03月, 研究代表者ウシ脂肪交雑形成機構の解明に向けた候補遺伝子変異の網羅的検証
- 科学研究費補助金／基盤研究(C), 2018年04月 - 2021年03月, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(B), 2017年04月 - 2020年03月
競争的資金
- 科学研究費補助金／基盤研究(B), 2016年04月 - 2020年03月
競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2016年04月 - 2018年03月
競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2014年04月 - 2016年03月
競争的資金
- 科学研究費補助金／基盤研究(C), 2012年04月 - 2015年03月
競争的資金
- 科学研究費補助金／基盤研究(B), 2011年04月 - 2014年03月
競争的資金
- 科学研究費補助金／若手研究(B), 2008年04月 - 2010年03月, 研究代表者
競争的資金