研究者紹介システム
| 川口 秀夫 |  |
| カワグチ ヒデオ | |
| 先端バイオ工学研究センター | |
| 准教授 | |
| 応用化学関係 |
研究活動
研究キーワード
研究分野
受賞
2021年10月 神戸大学, 令和３年度学長表彰（財務貢献者）
2020年10月 神戸大学, 令和２年度学長表彰（財務貢献者）
2019年10月 神戸大学, 令和元年度学長表彰（財務貢献者）
2013年08月 RSC Publishing, Green Chemistry Poster Prize
ベルギー王国出版社・新聞社・財団等の賞
論文
- Elsevier BV, 2022年01月, Bioresource Technology, 344 (Pt B), 126165, 英語
[査読有り]
研究論文（学術雑誌）
Abstract Background Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics.Results Under aerobic conditions, a recombinantCorynebacterium glutamicum strain KT01 expressinggriH andgriI genes derived fromStreptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. Anldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture.Conclusions Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.Graphical Abstract [査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2021年08月, Journal of Bioscience and Bioengineering, 132 (2), 140 - 147, 英語
[査読有り]
研究論文（学術雑誌）
Abstract The soil microbe Corynebacterium glutamicum is a leading workhorse in industrial biotechnology and has become famous for its power to synthetise amino acids and a range of bulk chemicals at high titre and yield. The product portfolio of the microbe is continuously expanding. Moreover, metabolically engineered strains of C. glutamicum produce more than 30 high value active ingredients, including signature molecules of raspberry, savoury, and orange flavours, sun blockers, anti-ageing sugars, and polymers for regenerative medicine. Herein, we highlight recent advances in engineering of the microbe into novel cell factories that overproduce these precious molecules from pioneering proofs-of-concept up to industrial productivity.Portland Press Ltd., 2021年06月07日, Essays in Biochemistry, 65 (2), 197 - 212, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- 2021年03月, Carbohydrate Polymers, 262 (15), 117938, 英語
[査読有り]
研究論文（学術雑誌）
Heterosis helps increase the biomass of many crops; however, while models for its mechanisms have been proposed, it is not yet fully understood. Here, we use a QTL analysis of the progeny of a high-biomass sorghum F1 hybrid to examine heterosis. Five QTLs were identified for culm length and were explained using the dominance model. Five resultant homozygous dominant alleles were used to develop pyramided lines, which produced biomasses like the original F1 line. Cloning of one of the uncharacterised genes (Dw7a) revealed that it encoded a MYB transcription factor, that was not yet proactively used in modern breeding, suggesting that combining classic dw1or dw3, and new (dw7a) genes is an important breeding strategy. In conclusion, heterosis is explained in this situation by the dominance model and a combination of genes that balance the shortness and early flowering of the parents, to produce F1 seed yields.
2021年02月25日, Scientific Reports, 11 (1), 4532 - 4532, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- Wiley, 2021年01月, Advanced Sustainable Systems, 5 (1), 2000193, 英語
[査読有り]
研究論文（学術雑誌）
ABSTRACT We determined the complete and draft genome sequences of two strains ofCorynebacterium glutamicum and revealed their genomic islands (GEIs). The two strains, ATCC 21799 and ATCC 31831, were found to have 3,079 and 3,109 coding sequences, respectively, with 13 GEIs each not present in the reference strain, ATCC 13032.American Society for Microbiology, 2020年08月06日, Microbiology Resource Announcements, 9 (32), e00430-20, 英語[査読有り]
研究論文（学術雑誌）
Controlling the carbon flux into a desired pathway is important for improving product yield in metabolic engineering. After entering a cell, glucose is channeled into glycolysis and the pentose phosphate pathway (PPP), which decreases the yield of target products whose synthesis relies on NADPH as a cofactor. Here, we demonstrate redirection of carbon flux into PPP under aerobic conditions in Corynebacterium glutamicum, achieved by replacing the promoter of glucose 6-phosphate isomerase gene (pgi) with an anaerobic-specific promoter of the lactate dehydrogenase gene (ldhA). The promoter replacement increased the split ratio of carbon flux into PPP from 39 to 83% under aerobic conditions. The titer, yield, and production rate of 1,5-diaminopentane, whose synthesis requires NADPH as a cofactor, were increased by 4.6-, 4.4-, and 2.6-fold, respectively. This is the largest improvement in the production of 1,5-diaminopentane or its precursor, lysine, reported to date. After aerobic cell growth, pgi expression was automatically induced under anaerobic conditions, altering the carbon flux from PPP to glycolysis, to produce succinate in a single metabolically engineered strain. Such an automatic redirection of metabolic pathway using an oxygen-responsive switch enables two-stage fermentation for efficient production of two different compounds by a single strain, potentially reducing the production costs and time for practical applications.
2020年04月17日, ACS Synthetic Biology, 9 (4), 814 - 826, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
3-Phenyllactic acid (PhLA) is useful as a start-up material in the pharmaceutical and biorefinery industries. To enhance the production of PhLA from glucose using recombinant Escherichia coli, the effects of glucose concentration and oxygen limitation on PhLA production are assessed in a fed-batch system using dissolved oxygen (DO)-stat method. The highest titer of PhLA (7.3 g L-1 ) is observed with a high concentration of glucose and under oxygen-limited conditions (DO = 0 ppm). Under oxygen limitation, cell growth and the formation of acetate and l-phenylalanine (Phe) by-products after 72 h of cultivation are reduced by 30%, 70%, and 81%, respectively, as compared to that under high DO conditions (DO = 5 ppm). Gene expression levels are compared between low and high DO conditions by quantitative polymerase chain reaction (qPCR) analysis. Several genes in the glycolysis (gapA and pykA), pentose phosphate (tktA), and early shikimate pathways for PhLA biosynthesis (aroF, aroG, and aroH) are upregulated under oxygen limitation. The results suggest that oxygen limitation affects metabolism in the shikimate pathway at both metabolic and transcriptional levels and that controlling the DO level is critical for enhanced production of a variety of aromatic compounds through the shikimate pathway.
2019年06月, Biotechnology Journal, 14 (6), 1800478, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.
2018年10月, Bioresource Technology, 265, 542 - 547, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.
2018年07月, Bioscience, Biotechnology, and Biochemistry, 82 (7), 1252 - 1259, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Background: l-Arabinose is the second most abundant component of hemicellulose in lignocellulosic biomass, next to d-xylose. However, few microorganisms are capable of utilizing pentoses, and catabolic genes and operons enabling bacterial utilization of pentoses are typically subject to carbon catabolite repression by more-preferred carbon sources, such as d-glucose, leading to a preferential utilization of d-glucose over pentoses. In order to simultaneously utilize both d-glucose and l-arabinose at the same rate, a modified metabolic pathway was rationally designed based on metabolome analysis. Results: Corynebacterium glutamicum ATCC 31831 utilized d-glucose and l-arabinose simultaneously at a low concentration (3.6g/L each) but preferentially utilized d-glucose over l-arabinose at a high concentration (15g/L each), although l-arabinose and d-glucose were consumed at comparable rates in the absence of the second carbon source. Metabolome analysis revealed that phosphofructokinase and pyruvate kinase were major bottlenecks for d-glucose and l-arabinose metabolism, respectively. Based on the results of metabolome analysis, a metabolic pathway was engineered by overexpressing pyruvate kinase in combination with deletion of araR, which encodes a repressor of l-arabinose uptake and catabolism. The recombinant strain utilized high concentrations of d-glucose and l-arabinose (15g/L each) at the same consumption rate. During simultaneous utilization of both carbon sources at high concentrations, intracellular levels of phosphoenolpyruvate declined and acetyl-CoA levels increased significantly as compared with the wild-type strain that preferentially utilized d-glucose. These results suggest that overexpression of pyruvate kinase in the araR deletion strain increased the specific consumption rate of l-arabinose and that citrate synthase activity becomes a new bottleneck in the engineered pathway during the simultaneous utilization of d-glucose and l-arabinose. Conclusions: Metabolome analysis identified potential bottlenecks in d-glucose and l-arabinose metabolism and was then applied to the following rational metabolic engineering. Manipulation of only two genes enabled simultaneous utilization of d-glucose and l-arabinose at the same rate in metabolically engineered C. glutamicum. This is the first report of rational metabolic design and engineering for simultaneous hexose and pentose utilization without inactivating the phosphotransferase system.
BioMed Central Ltd., 2018年05月17日, Microbial Cell Factories, 17 (1), 76, 英語[査読有り]
研究論文（学術雑誌）
Measuring the concentrations of metabolites and estimating the reaction rates of each reaction step consisting of metabolic pathways are significant for an improvement in microorganisms used in maximizing the production of materials. Although the reaction pathway must be identified for such an improvement, doing so is not easy. Numerous reaction steps have been reported; however, the actual reaction steps activated vary or change according to the conditions. Furthermore, to build mathematical models for a dynamical analysis, the reaction mechanisms and parameter values must be known; however, to date, sufficient information has yet to be published for many cases. In addition, experimental observations are expensive. A new mathematical approach that is applicable to small sample data, and that requires no detailed reaction information, is strongly needed. S-system is one such model that can use smaller samples than other ordinary differential equation models. We propose a simplified S-system to apply minimal quantities of samples for a dynamic analysis of the metabolic pathways. We applied the model to the phenyl lactate production pathway of Escherichia coli. The model obtained suggests that actually activated reaction steps and feedback are inhibitions within the pathway.
2018年05月, Bioinformatics and Biology Insights, 12, 1 - 7, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
© 2017 We investigated the use of low concentrations of butanol (<40%, all v/v) as an organosolv pretreatment to fractionate lignocellulosic biomass into cellulose, hemicellulose, and lignin. The pretreatment conditions were optimized for sorghum bagasse by focusing on four parameters: butanol concentration, sulfuric acid concentration, pretreatment temperature, and pretreatment time. A butanol concentration of 25% or higher together with 0.5% or higher acid was effective for removing lignin while retaining most of the cellulose in the solid fraction. The highest cellulose (84.9%) and low lignin (15.3%) content were obtained after pretreatment at 200 °C for 60 min. Thus, pretreatment comprising 25% butanol, 0.5% acid, 200 °C, and 60 min process time was considered optimal. Enzymatic saccharification and fermentation by Saccharomyces cerevisiae produced 61.9 g/L ethanol from 200 g/L solid fraction obtained following pretreatment, and 10.2 g/L ethanol was obtained from the liquid fraction by xylose-utilizing S. cerevisiae following membrane nanofiltration to remove butanol.
2018年03月, Bioresource Technology, 252, 157 - 164, 英語[査読有り]
研究論文（学術雑誌）
Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by expressing the genes encoding a tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344 and 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (≤2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.
一般社団法人 日本エネルギー学会, 2018年, バイオマス科学会議発表論文集, 13 (0), 135 - 136, 日本語[査読有り]
The worldwide market for plastics is rapidly growing, and plastics polymers are typically produced from petroleum-based chemicals. The overdependence on petroleum-based chemicals for polymer production raises economic and environmental sustainability concerns. Recent progress in metabolic engineering has expanded fermentation products from existing aliphatic acids or alcohols to include aromatic compounds. This diversity provides an opportunity to expand the development and industrial uses of high-performance bio-based polymers. However, most of the biomonomers are produced from edible sugars or starches that compete directly with food and feed uses. The present review focuses on recent progress in the microbial conversion of biomass into bio-based polymers, in which fermentative products from renewable feedstocks serve as biomonomers for the synthesis of bio-based polymers. In particular, the production of biomonomers from inedible lignocellulosic feedstocks by metabolically engineered microorganisms and the synthesis of bio-based engineered plastics from the biological resources are discussed. (C) 2017 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2017年12月, Bioresource Technology, 245 (Pt B), 1664 - 1673, 英語[査読有り]
研究論文（学術雑誌）
The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.
2017年08月, Applied Microbiology and Biotechnology, 101 (15), 6007 - 6014, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and a precursor for biologically active compounds and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by introducing the genes encoding a 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa and tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (ae<currency>2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. In SHF, the formation of by-product acetate and the accumulation of potential fermentation inhibitors increased significantly with kraft pulp hydrolysate than filter paper hydrolysate. The combination of these inhibitors had synergistic effects on caffeic acid fermentation at low concentrations. With lower loads of cellulase in SSF, less potential fermentation inhibitors (furfural, 5-hydroxymethyfurfural, and 4-hydroxylbenzoic acid) accumulated in the medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.
SPRINGER, 2017年07月, Applied Microbiology and Biotechnology, 101 (13), 5279 - 5290, 英語[査読有り]
研究論文（学術雑誌）
Bio-refinery processes require use of the most suitable lignocellulosic biomass for enzymatic saccharification and microbial fermentation. Glucose yield from biomass solid fractions obtained after dilute sulfuric acid (1%) pretreatment (at 180 degrees C) was investigated using 14, 8, and 16 varieties of rice, wheat, and sorghum, respectively. Biomass solid fractions of each crop showed similar cellulose content. However, glucose yield after enzymatic hydrolysis (cellulase loading at 6.6 filter paper unit/g-biomass) was different among the varieties of each crop, indicating genotypic differences for rice, wheat, and sorghum. Nuclear magnetic resonance method revealed that the high residual level of lignin aromatic regions decreased glucose yield from solid fraction of sorghum.
TAYLOR & FRANCIS LTD, 2017年, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81 (8), 1650 - 1656, 英語[査読有り]
研究論文（学術雑誌）
The feedstocks used for the production of bio-based chemicals have recently expanded from edible sugars to inedible and more recalcitrant forms of lignocellulosic biomass. To produce biobased chemicals from renewable polysaccharides, several bioprocessing approaches have been developed and include separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP). In the last decade, SHF, SSF, and CBP have been used to generate macromolecules and aliphatic and aromatic compounds that are capable of serving as sustainable, drop-in substitutes for petroleum-based chemicals. The present review focuses on recent progress in the bioprocessing of microbially produced chemicals from renewable feedstocks, including starch and lignocellulosic biomass. In particular, the technological feasibility of bio-based chemical production is discussed in terms of the feedstocks and different bioprocessing approaches, including the consolidation of enzyme production, enzymatic hydrolysis of biomass, and fermentation.
CURRENT BIOLOGY LTD, 2016年12月, Current Opinion in Biotechnology, 42, 30 - 39, 英語[査読有り][招待有り]
研究論文（学術雑誌）
Rice straw is a promising resource for bioethanol production. Because the glucose content of pretreatment liquid hydrolysates is highly correlated with ethanol yield, the selection of appropriate rice cultivars is essential. The glucose content in liquid hydrolysates of pretreated rice straws of 208 diverse cultivars was evaluated in natural field in 2013 and 2014 using a novel high-throughput system. The glucose content of the rice straw samples varied across cultivars and was affected by environmental factors such as temperature and solar radiation. Several high-quality cultivars exhibiting high glucose content in both years were identified. The results of this study can aid in development of novel rice cultivars suitable as both feedstocks for bioethanol production and cooking.
TAYLOR & FRANCIS LTD, 2016年05月, Bioscience Biotechnology and Biochemistry, 80 (5), 863 - 869, 英語[査読有り]
研究論文（学術雑誌）
Lignocellulosic hydrolysates contain compounds that inhibit microbial growth and fermentation, thereby decreasing the productivity of biofuel and biochemical production. In particular, the heterocyclic aldehyde furfural is one of the most toxic compounds found in these hydrolysates. We previously demonstrated that Corynebacterium glutamicum converts furfural into the less toxic compounds furfuryl alcohol and 2-furoic acid. To date, however, the genes involved in these oxidation and reduction reactions have not been identified in the C. glutamicum genome. Here, we show that Cgl0331 (designated FudC) is mainly responsible for the reduction of furfural into furfuryl alcohol in C. glutamicum. Deletion of the gene encoding FudC markedly diminished the in vivo reduction of furfural to furfuryl alcohol. Purified His-tagged FudC protein from Escherichia coli was also shown to convert furfural into furfuryl alcohol in an in vitro reaction utilizing NADPH, but not NADH, as a cofactor. Kinetic measurements demonstrated that FudC has a high affinity for furfural but has a narrow substrate range for other aldehydes compared to the protein responsible for furfural reduction in E. coli.
SPRINGER, 2016年03月, Applied Microbiology and Biotechnology, 100 (6), 2685 - 2692, 英語[査読有り]
研究論文（学術雑誌）
Background: The primary components of lignocellulosic biomass such as sorghum bagasse are cellulose, hemicellulose, and lignin. Each component can be utilized as a sustainable resource for producing biofuels and bio-based products. However, due to their complicated structures, fractionation of lignocellulosic biomass components is required. Organosolv pretreatment is an attractive method for this purpose. However, as organosolv pretreatment uses high concentrations of organic solvents (>50 %), decreasing the concentration necessary for fractionation would help reduce processing costs. In this study, we sought to identify organic solvents capable of efficiently fractionating sorghum bagasse components at low concentrations. Results: Five alcohols (ethanol, 1-propanol, 2-propanol, 1-butanol, and 1-pentanol) were used for organosolv pretreatment of sorghum bagasse at a concentration of 12.5 %. Sulfuric acid (1 %) was used as a catalyst. With 1-butanol and 1-pentanol, three fractions (black liquor, liquid fraction containing xylose, and cellulose-enriched solid fraction) were obtained after pretreatment. Two-dimensional nuclear magnetic resonance analysis revealed that the lignin aromatic components of raw sorghum bagasse were concentrated in the black liquor fraction, although the major lignin side-chain (beta-O-4 linkage) was lost. Pretreatment with 1-butanol or 1-pentanol effectively removed p-coumarate, some guaiacyl, and syringyl. Compared with using no solvent, pretreatment with 1-butanol or 1-pentanol resulted in two-fold greater ethanol production from the solid fraction by Saccharomyces cerevisiae. Conclusions: Our results revealed that a low concentration (12.5 %) of a highly hydrophobic solvent such as 1-butanol or 1-pentanol can be used to separate the black liquor from the solid and liquid fractions. The efficient delignification and visible separation of the lignin-rich fraction possible with this method simplify the fractionation of sorghum bagasse.
BIOMED CENTRAL LTD, 2016年02月, Biotechnology for Biofuels, 9, 27, 英語[査読有り]
研究論文（学術雑誌）
Four types of kenaf bast fibers were prepared via a combination of Wise treatments, for delignification, and alkaline treatments, for the removal of hemicellulose. Each type of kenaf bast fiber with different refining processes were nano fibrillated by grinding. Resulting, cellulose nanofiber (CNF) sheet was obtained from CNF by vacuum filtration (Scheme 1). The structures and properties of these CNF sheets then were investigated to determine how the CNF components had affected these properties. All of the CNFs from different refining processes were classified as a cellulose I-beta type by X-ray diffraction. However, the mechanical properties (Young's modulus, tensile strength and toughness) of the CNF sheet with Wise treatment were higher than the properties of the other three CNF sheets. These results strongly suggested that alkaline treatment was unnecessary for the removal of hemicellulose, and that the application of the Wise treatment effectively imparted high mechanical properties to the cellulose microfiber.
SPRINGER, 2016年02月, Cellulose, 23 (1), 403 - 414, 英語[査読有り]
研究論文（学術雑誌）
Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.
一般社団法人 日本エネルギー学会, 2016年01月, バイオマス科学会議発表論文集, 11, 35 - 36, 日本語[査読有り]
研究論文（国際会議プロシーディングス）
This study aimed to optimize extracellular glutathione production by a Saccharomyces cerevisiae engineered strain and to concentrate the extracellular glutathione by membrane separation processes, including ultrafiltration (UF) and nanofiltration (NF). Synthetic defined (SD) medium containing 20 g L-1 glucose was fermented for 48 h; the fermentation liquid was passed through an UF membrane to remove macromolecules. Glutathione in this permeate was concentrated for 48 h to 545.1 +/- 33.6 mg L-1 using the NF membrane; this was a significantly higher concentration than that obtained with yeast extract peptone dextrose (YPD) medium following 96 h NF concentration (217.9 +/- 57.4 mg L-1). This higher glutathione concentration results from lower cellular growth in SD medium (final OD600 = 6.9 +/- 0.1) than in YPD medium (final OD600 = 11.0 +/- 0.6) and thus higher production of extracellular glutathione (16.0 +/- 1.3 compared to 9.2 +/- 2.1 mg L-1 in YPD medium, respectively). Similar fermentation and membrane processing of sweet sorghum juice containing 20 g L-1 total sugars provided 240.3 +/- 60.6 mg L-1 glutathione. Increased extracellular production of glutathione by this engineered strain in SD medium and subsequent UF permeation and NF concentration in shortend time may help realize industrial recovery of extracellular glutathione. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2016年01月, Journal of Bioscience and Bioengineering, 121 (1), 96 - 100, 英語[査読有り]
研究論文（学術雑誌）
Synthetic polymers are widely used in daily life. Due to increasing environmental concerns related to global warming and the depletion of oil reserves, the development of microbial-based fermentation processes for the production of polymer building block chemicals from renewable resources is desirable to replace current petroleum-based methods. To this end, strains that efficiently produce the target chemicals at high yields and productivity are needed. Recent advances in metabolic engineering have enabled the biosynthesis of polymer compounds at high yield and productivities by governing the carbon flux towards the target chemicals. Using these methods, microbial strains have been engineered to produce monomer chemicals for replacing traditional petroleum-derived aliphatic polymers. These developments also raise the possibility of microbial production of aromatic chemicals for synthesizing high-performance polymers with desirable properties, such as ultraviolet absorbance, high thermal resistance, and mechanical strength. In the present review, we summarize recent progress in metabolic engineering approaches to optimize microbial strains for producing building blocks to synthesize aliphatic and high-performance aromatic polymers.
BIOMED CENTRAL LTD, 2016年01月, Microbial Cell Factories, 15, 19, 英語[査読有り]
研究論文（学術雑誌）
The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0 g l(-1)) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum. (C) 2015 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2015年12月, Bioresource Technology, 198, 410 - 417, 英語[査読有り]
研究論文（学術雑誌）
A renewable raw material, rice straw is pretreated for biorefinery usage. Solution-state two-dimensional (2D) H-1-(13) C hetero-nuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy, was used to analyze 13 cultivars of rice straw before and after dilute acid pretreatment, to characterize general changes in the lignin and polysaccharide components. Intensities of most (15 of 16) peaks related to lignin aromatic regions, such as p-coumarate, guaiacyl, syringyl, p-hydroxyphenyl, and cinnamyl alcohol, and methoxyl, increased or remained unchanged after pretreatment. In contrast, intensities of most (11 of 13) peaks related to lignin aliphatic linkages or ferulate decreased. Decreased heterogeneity in the intensities of three peaks related to cellulose components in acid-insoluble residues resulted in similar glucose yield (0.45-0.59 g/g-dry biomass). Starch-derived components showed positive correlations (r = 0.71 to 0.96) with glucose, 5-hydroxymethyl-furfural (5-HMF), and formate concentrations in the liquid hydrolysates, and negative correlations (r = -0.95 to -0.97) with xylose concentration and acid-insoluble residue yield. These results showed the fate of lignin and polysaccharide components by pretreatment, suggesting that lignin aromatic regions and cellulose components were retained in the acid insoluble residues and starch-derived components were transformed into glucose, 5-HMF, and formate in the liquid hydrolysate.
PUBLIC LIBRARY SCIENCE, 2015年06月, PLoS ONE, 10 (6), e0128417, 英語[査読有り]
研究論文（学術雑誌）
Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation (ultrafiltration permeation and nanofiltration concentration) to increase sugar contents, was investigated. Ethanol production at 5th batch fermentation by Saccharomyces cerevisiae BY4741 attained 113.7 +/- 3.1 g L-1 (89.1 +/- 2.2% of the theoretical ethanol yield) from 270.0 +/- 22.6 g L-1 sugars, corresponding to 98.7% of ethanol titer attained at the 1st batch fermentation. This titer was comparable to ethanol production of 115.8 +/- 0.6 g L-1 (87.1 +/- 2.7% of the theoretical ethanol yield) obtained at 5th batch fermentation with 3 g L-1 yeast extract and 6 g L-1 polypeptone. Increase of cell density in the concentrated sweet sorghum juice was observed during sequential batch fermentation, as indicated by increased OD600. Utilization of sweet sorghum juice as the sole source, membrane separation, and S. cerevisiae was a cost-effective process for high ethanol production. (C) 2015 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2015年06月, Bioresource Technology, 186, 351 - 355, 英語[査読有り]
研究論文（学術雑誌）
Background: Hydrothermal pretreatment of lignocellulosic biomass such as rice straw can dissolve part of the lignin and hemicellulose into a liquid fraction, thus facilitating enzyme accessibility to cellulose in bioethanol production process. Lignin is awaited to be recovered after hydrothermal pretreatment for utilization as value-added chemical, and lignin recovery also means removal of fermentation inhibitors. To recover lignin with high content from the liquid fraction, it is necessary to separate lignin and hemicellulose-derived polysaccharide. Therefore, the following processes were applied: membrane separation with nanofiltration (NF) and enzymatic hydrolysis by hemicellulase. To clarify lignin-concentrated fraction obtained during these processes, the fates of lignin and polysaccharide components were pursued by a solution NMR method and confirmed by compositional analysis of each fraction. Results: After hydrothermal pretreatment of rice straw, the NF concentrate of the supernatant of liquid fraction was hydrolyzed by hemicellulase and the resulting black precipitate was recovered. In this black precipitate, the intensity of NMR spectra related to lignin aromatic regions increased and those related to polysaccharides decreased, compared to rice straw, the solid fraction after hydrothermal pretreatment, and the NF concentrate. The lignin content of the black precipitate was 65.8 %. Lignin in the black precipitate included 52.9 % of the acid-insoluble lignin and 19.4 % of the soluble lignin in the NF concentrate of supernatant of liquid fraction. Conclusion: A precipitate with high lignin content was obtained from supernatants of the liquid fraction. These results suggested that precipitation of lignin was enhanced from concentrated mixtures of lignin and hemicellulosic polysaccharides by hydrolyzing the polysaccharides. Precipitation of lignin can contribute to lignin recovery from lignocellulosic biomass and, at the same time, allow more efficient ethanol production in the subsequent fermentation process.
BIOMED CENTRAL LTD, 2015年06月, Biotechnology for Biofuels, 8, 88, 英語[査読有り]
研究論文（学術雑誌）
Dilute acid-pretreated sorghum bagasse, which was predominantly composed of glucan (59%) and xylose (7.2%), was used as a lignocellulosic feedstock for D-phenyllactic acid (PhLA) production by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. During fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, the PhLA yield was reduced by 35% compared to filter paper hydrolysate, and metabolomics analysis revealed that NAD(P)H regeneration and intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate for PhLA biosynthesis markedly reduced. Compared to separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components, including p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual enzymatic hydrolysis during SSF enhances PhLA production under glucose limitation and reduces the accumulation of fermentation inhibitors, collectively leading to increased PhLA yield. (c) 2015 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2015年04月, Bioresource Technology, 182, 169 - 178, 英語[査読有り]
研究論文（学術雑誌）
The aim of this investigation was to attain high ethanol concentration by concentrating sweet sorghum juice using a two-step membrane separation process. Ultrafiltration permeation of the juice was used to remove residues, followed by nanofiltration concentration to increase the sugar concentration. The concentrated juice containing 180.0 g L-1 sucrose, 59.3 g L-1 glucose and 49.3 g L-1 fructose supplemented with nitrogen sources (10 and 20 g L-1 of yeast extract and polypeptone, respectively) was fermented by Saccharomyces cerevisiae BY4741 to produce 133.5 g L-1 of ethanol (87.6% of theoretical yield) after 48 h fermentation. Importantly, the addition of lower concentrations of exogenous nitrogen sources (3 and 6 g L-1 of yeast extract and polypeptone, respectively) or no exogenous nitrogen sources resulted in the production of 131.4 and 132.8 g L-1 of ethanol (84.8% and 86.0% of theoretical yield), respectively, after 48 h fermentation. (C) 2014 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 2014年10月, Bioresource Technology, 169, 821 - 825, 英語[査読有り]
研究論文（学術雑誌）
Simultaneous saccharification and fermentation (SSF) of renewable cellulose for the production of 3-phenyllactic acid (PhLA) by recombinant Escherichia coli was investigated. Kraft pulp recovered from biomass fractionation processes was used as a model cellulosic feedstock and was hydrolyzed using 10-50 filter paper unit (FPU) g(-1) kraft pulp of a commercial cellulase mixture, which increased the glucose yield from 21% to 72% in an enzyme dose-dependent manner. PhLA fermentation of the hydrolyzed kraft pulp by a recombinant E. coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens TK1 produced 1.9 mM PhLA. The PhLA yield obtained using separate hydrolysis and fermentation was enhanced from 5.8% to 42% by process integration into SSF of kraft pulp (20 g L-1) in a complex medium (pH 7.0) at 37 degrees C The PhLA yield was negatively correlated with the initial glucose concentration, with a five-fold higher PhLA yield observed in culture medium containing log L-1 glucose compared to 100 g L-1. Taken together, these results suggest that the PhLA yield from cellulose in kraft pulp can be improved by SSF under glucose-limited conditions. (C) 2014 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 2014年07月, Biochemical Engineering Journal, 88, 188 - 194, 英語[査読有り]
研究論文（学術雑誌）
To develop cost-effective systems for d-lactate production, here, the effect of high-cell density cultivation of metabolically engineered Lactobacillus plantarum on d-lactate production was evaluated. A xylose-assimilating strain of L. plantarum was anaerobically cultured with mixed sugars (glucose and xylose) as substrates. Compared to undiluted nutrient-rich de Man, Rogosa, and Sharpe (MRS) medium, d-lactate production by cultivating in 10-fold diluted MRS (0.1 MRS) medium or normal saline solution was 89.7 and 81.3 %, respectively. Notably, the xylose consumption rate was comparable in the three cultures, whereas the glucose consumption rate decreased by 18.3 and 26.1 % in 0.1 MRS medium and normal saline solution, respectively, resulting in a reduction of the d-lactate production rate. The d-lactate productivity in high-cell density cultivation was proportional to the initial cell concentrations. The use of a two-step cultivation process involving growing and resting cells in a single bioreactor revealed that the ratio of the glucose and xylose consumption rates (based on grams consumed) in resting cell conditions was 1.88, whereas that in growing conditions was 2.58. Cultivation of L. plantarum in growing conditions for 24 h produced 73.2 g/l d-lactate with the yield of 0.90 g/g, whereas cells cultivation under resting cell conditions in a saline solution for 24 h produced 68.7 g/l d-lactate with the yield of 0.93 g/g. In total, 141.9 g/l d-lactate was produced after 48 h cultivation, a value that represents the highest reported concentration of d-lactate produced from mixed sugars to date. Our findings contribute to the cost-effective, large-scale production of d-lactate.
SPRINGER, 2014年06月, Applied Microbiology and Biotechnology, 98 (11), 4911 - 4918, 英語[査読有り]
研究論文（学術雑誌）
- 2014年04月, バイオインダストリー, 31 (4), 62 - 68, 日本語植物の機能解明と新たな機能材料創出の可能性
研究論文（学術雑誌）
To exploit the potential diversity of thermophilic exoelectrogens, two-chamber microbial fuel cells (MFCs) were inoculated with thermophilic anaerobic digester sludge and operated at 55 °C without supplementing with exogenous redox mediator. The MFC generated a maximum power density of 823 mW m-2 after 200 h of operation. Molecular phylogenetic analyses suggested that the microbial population on the anode was dominated by a species closely related to a thermophilic nitrate-reducing bacterium Calditerrivibrio nitroreducens, for which a strain (Yu37-1) has been isolated in pure culture. Thus, a pure culture of the C. nitroreducens strain Yu37-1 was inoculated into MFC to examine the electricity generation capability. Without an exogenous mediator, MFCs stably produced electricity with a maximum power density of 272 mW m-2 for > 400 h of operation. The MFC current recovered to the original level within few hours after medium replacement, suggesting that the electricity generation was caused by the anodic microorganisms. Cyclic voltammetry indicated that redox systems (E3 and Ec) with similar potentials (-0.14 and -0.17 V) made the main contributions to the exoelectrogenic activities of the sludge-derived consortium and C. nitroreducens Yu37-1, respectively. This study undertook the bioelectrochemical characterization of C. nitroreducens as the first example of a thermophilic Gram-negative exoelectrogen. © 2013 American Chemical Society.
2013年11月05日, Environmental Science and Technology, 47 (21), 12583 - 12590, 英語[査読有り]
研究論文（学術雑誌）
Electromethanogenesis is a new bio-electrochemical reaction potentially useful for energy conversion. As a first step toward its technical application, electromethanogenic reactors were built, and their bio-electrochemical properties were analyzed. Comparisons of the microbial compositions of the electromethanogenic cathode and the current-producing anode suggested an electromethanogenic pathway mediated by exoelectrogenic bacteria. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2013年07月, Journal of Bioscience and Bioengineering, 116 (1), 114 - 117, 英語[査読有り]
研究論文（学術雑誌）
To explore diversity of thermophilic exoelectrogens, a thermophilic microbial fuel cell was constructed. Population analysis of the anodic microorganisms suggested possible involvement of Caloramator-related bacteria in electricity generation. Pure culture of Caloramator australicus showed electricity-generating ability, indicating that the bacterium is a new thermophilic exoelectrogen. © 2012 The Society for Biotechnology, Japan.
2013年03月, Journal of Bioscience and Bioengineering, 115 (3), 268 - 271, 英語[査読有り]
研究論文（学術雑誌）
Geological storage of carbon dioxide (CO2) as currently conceived is not commercially viable. To promote deployment of CO2 capture and storage (CCS), substantial value must be added to CCS operations. We have proposed a subterranean carbon plantation that involves storing CO2 in a geological reservoir, biologically converting the stored CO2 to methane in situ, and harvesting the biogenic methane as a recycled energy source. To examine the durability of methanogenic metabolism under storage reservoir conditions, the methanogenic activity of Methanothermobacter thermautotrophicus (a representative subsurface methanogen) was assessed under nutrient-limited and reduced-pH conditions in actual formation-water-based media. Moreover, to examine the possibility of electrochemically supplying the source of reducing power into the reservoir, methanogen was also incubated in absence of exogenously supplied molecular hydrogen with applied voltage. Applied-voltage-dependent methanogenesis was observed, suggesting that methanogen can utilize electrons and protons as a reducing-power source to reduce CO2 to methane. Towards practical deployment of the electromethanogenic system to utilize CCS reservoirs as energy-reserving tanks, further studies are required to enhance the bio-electromethanogenic activity and optimize well configurations. (C) 2012 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2013年02月, Energy Conversion and Management, 66, 343 - 350, 英語[査読有り]
研究論文（学術雑誌）
To establish a biotechnological system to convert CO2 into methane, we are trying to develop a new CO2 bio-conversion technology based on "electromethanogenesis", a new bio-electrolysis reaction using microbially-catalyzed electrode. In this study, we characterized bio-electrochemical properties of electromethanogenic reaction by Methanothermobacter thermautotrophicus strain ΔH, a thermophilic methanogen. The methanogen can electromethanogenically produce methane without exogenouslysupplied hydrogen. In the reaction, the methanogen utilized molecular hydrogen, which was evolved by the abiotic electrochemical reaction, for the hydrogenotrophic methanogenesis. The current-to-methane conversion efficiency was 20% and the hydrogen evolution reaction was the rate-limiting step of the reaction.
Elsevier Ltd, 2013年, Energy Procedia, 37, 7021 - 7028, 英語[査読有り]
研究論文（国際会議プロシーディングス）
Biological electromethanogenesis is a promising technology which can be applicable to convert geologically-stored CO2 into CH4 by electrochemical active microorganisms as biocatalysts. To gain biocatalysts applicable within CO2-storage reservoirs, a Microbial Fuel Cell (MFC) was used to enrich electrochemical active microorganisms in reservoir brine sample from a petroleum reservoir. The MFC began to produce electricity after 25 hours of inoculation and reached the first maximum current at 50 hours post inoculation. The maximum power density of the thermophilic MFC was ca. 1003 mW/m2, higher than that of most thermophilic MFCs reported previously, suggesting that subsurface reservoir harbors highly functional biocatalysts.
Elsevier Ltd, 2013年, Energy Procedia, 37, 7006 - 7013, 英語[査読有り]
研究論文（国際会議プロシーディングス）
To develop a technological system to add substantial value to CCS operations, we are proposing a system to employ a new bio-electrochemical reaction, called "electromethanogenesis", to convert geologically-stored CO2 into methane, a recycled energy source. In this study, we showed that microorganisms derived from a subsurface reservoir were electromethanogenically active. Moreover, the microbial consortium selectively enriched based on electrochemical activity had the highest electromethanogenic activity reported so far. Thus, our study indicated that, for the electromethanogenic conversion of geologically-stored CO2, recruitment of microorganisms endogenous to the reservoir was an effective strategy.
Elsevier Ltd, 2013年, Energy Procedia, 37, 7014 - 7020, 英語[査読有り]
研究論文（国際会議プロシーディングス）
The in situ oil sands production method called steam-assisted gravity drainage (SAGD) reuses process wastewater following treatment. However, the treatment and reuse processes concentrate contaminants in the process water. To determine the concentration and dynamics of inorganic and organic contaminants, makeup water and process water from six process steps were sampled at a facility employing the SAGD process in Alberta, Canada. In the groundwater used for the makeup water, the total dissolved organic carbon (DOC) content was 4 mg/L. This significantly increased to 508 mg/L in the produced water, followed by a gradual increase with successive steps in subsequent water treatments. The concentrations and dynamics of DOC constituents in the process water determined by gas chromatography-mass spectrometry showed that in the produced water, volatile organic compounds (VOCs) such as acetone (33.1 mg/L) and 2-butanone (13.4 mg/L) predominated, and there were significant amounts of phenolic compounds (total 9.8 mg/L) and organic acids including naphthenic acids (NAs) corresponding to the formula CnH2n+zOx for combinations of n = 4 to 18, Z = 0 and -2, and X = 2 to 4 (53 mg/L) with trace amounts of polycyclic aromatic hydrocarbons (PAHs) such as naphthalene and phenanthrene. No organic contaminants, except for saturated fatty acids, were detected in the groundwater. The concentration of DOC in the recycled water was 4.4-fold higher than that in the produced water. Likewise, the total concentrations of phenols and organic acids in the recycled water were 1.7- and 4.5-fold higher than in the produced water, whereas the total concentrations of VOCs and PAHs in the recycled water were reduced by over 80%, suggesting that phenols and organic acids are selectively concentrated in the process water treatment. This comprehensive chemical analysis thus identified organic constituents that were concentrated in the process water and which interfere with subsequent water treatments in the SAGD process. (C) 2012 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2012年11月, Water Research, 46 (17), 5566 - 5574, 英語[査読有り]
研究論文（学術雑誌）
An organic solvent-tolerant bacterium, Rhodococcus opacus B-4, was metabolically engineered to remove sulfur from dibenzothiophene (DBT), a component of crude oil. The resulting recombinant strain ROD2-8 constitutively expressed the Rhodococcus erythropolis IGTS8 genes dszA, dszB, and dszC, encoding dibenzothiophene sulfone monooxygenase, 2-(2'-hydroxyphenyl) benzenesulfinate desulfinase, and dibenzothiophene monooxygenase, respectively, of the 45 pathway to avoid transcriptional inhibition by the sulfate end-product. Unlike the wild-type strain, ROD2-8 grew in mineral salts medium containing DBT as the sole sulfur source. Under aqueous conditions, ROD2-8 resting cells converted greater than 85% of DBT to 2-hydroxybiphenyl (2-HBP), although the consumption rate by ROD2-8 cells precultured on DBT as the sole sulfur source was 3.3-fold higher than that of cells cultured in complex medium. Notably, DBT consumption rates increased by 80% in oil-water biphasic reaction mixtures with n-hexadecane as the organic solvent, and resting cells were predominantly localized in the emulsion layer. Desulfurization activity in biphasic reaction mixtures increased with increasing concentrations of DBT and was not markedly inhibited by 2-HBP accumulation. Intracellular concentrations of DBT and 2-HBP were significantly lower under biphasic conditions than aqueous conditions. Our findings suggest that the enhanced desulfurization activity under biphasic conditions results from the combined effects of attenuated feedback inhibition and reduced mass transfer limitations due to 2-HBP diffusion from cells and accumulation of both substrate and biocatalyst in the emulsion layer, respectively. Therefore, the solvent-tolerant and hydrophobic bacterium R. opacus B-4 appears suitable for biodesulfurization reactions in solvents containing a minimum ratio of water. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年03月, Journal of Bioscience and Bioengineering, 113 (3), 360 - 366, 英語[査読有り]
研究論文（学術雑誌）
The diversity of microbial communities associated with non-water-flooded high-temperature reservoir of the Niibori oilfield was characterized. Analysis of saturated hydrocarbons revealed that n-alkanes in crude oil from the reservoir were selectively depleted, suggesting that crude oil might be mildly biodegraded in the reservoir. To examine if any specific microorganism(s) preferentially attached to the crude oil or the other components (large insoluble particles and formation water) of the reservoir fluid, 16S rRNA gene clone libraries were constructed from each component of the reservoir fluid. The clones in the archaeal libraries (414 clones in total) represented 16 phylotypes, many of which were closely related to methanogens. The bacterial libraries (700 clones in total) were composed of 49 phylotypes belonging to one of 16 phylum-level groupings, with Firmicutes containing the greatest diversity of the phylotypes. In the crude-oil- and large-insoluble-particle-associated communities, a Methanosaeta-related phylotype dominated the archaeal sequences, whereas hydrogenotrophic methanogens occupied a major portion of sequences in the library of the formation-water-associated community. The crude-oil associated bacterial community showed the largest diversity, containing 35 phylotypes, 16 of which were not detected in the other bacterial communities. Thus, although the populations associated with the reservoir-fluid components largely shared common phylogenetic context, a specific fraction of microbial species preferentially attached to the crude oil and insoluble particles. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年02月, Journal of Bioscience and Bioengineering, 113 (2), 204 - 210, 英語[査読有り]
研究論文（学術雑誌）
We examined methane production by microorganisms collected from a depleted oilfield. Our results indicated that microorganisms indigenous to the petroleum reservoir could effectively utilize yeast extract, suggesting that the indigenous microorganisms and proteinaceous nutrients could be recruitable for Microbially Enhanced Oil Recovery. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年01月, Journal of Bioscience and Bioengineering, 113 (1), 84 - 87, 英語[査読有り]
研究論文（学術雑誌）
To recover energy from carbon dioxide sequestered in geological reservoirs, the geochemical effects of acidic and substrate- and nutrient-limiting conditions on methane production by the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus were investigated in a simulated deep saline aquifer environment using formation water media retrieved from petroleum reservoirs. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2010年07月, Journal of Bioscience and Bioengineering, 110 (1), 106 - 108, 英語[査読有り]
研究論文（学術雑誌）
Corynebacterium glutamicum strains CRA1 and CRX2 are able to grow on l-arabinose and d-xylose, respectively, as sole carbon sources. Nevertheless, they exhibit the major shortcoming that their sugar consumption appreciably declines at lower concentrations of these substrates. To address this, the C. glutamicum ATCC31831 l-arabinose transporter gene, araE, was independently integrated into both strains. Unlike its parental strain, resultant CRA1-araE was able to aerobically grow at low (3.6 g center dot l(-1)) l-arabinose concentrations. Interestingly, strain CRX2-araE grew 2.9-fold faster than parental CRX2 at low (3.6 g center dot l(-1)) d-xylose concentrations. The corresponding substrate consumption rates of CRA1-araE and CRX2-araE under oxygen-deprived conditions were 2.8- and 2.7-fold, respectively, higher than those of their respective parental strains. Moreover, CRA1-araE and CRX2-araE utilized their respective substrates simultaneously with d-glucose under both aerobic and oxygen-deprived conditions. Based on these observations, a platform strain, ACX-araE, for C. glutamicum-based mixed sugar utilization was designed. It harbored araBAD for l-arabinose metabolism, xylAB for d-xylose metabolism, d-cellobiose permease-encoding bglF (317A) , beta-glucosidase-encoding bglA and araE in its chromosomal DNA. In mineral medium containing a sugar mixture of d-glucose, d-xylose, l-arabinose, and d-cellobiose under oxygen-deprived conditions, strain ACX-araE simultaneously and completely consumed all sugars.
SPRINGER, 2009年11月, Applied Microbiology and Biotechnology, 85 (1), 105 - 115, 英語[査読有り]
研究論文（学術雑誌）
Corynebacterium glutamicum ATCC 31831 grew on L-arabinose as the sole carbon source at a specific growth rate that was twice that on D-glucose. The gene cluster responsible for L-arabinose utilization comprised a six-cistron transcriptional unit with a total length of 7.8 kb. Three L-arabinose-catabolizing genes, araA (encoding L-arabinose isomerase), araB (L-ribulokinase), and araD (L-ribulose-5-phosphate4-epimerase), comprised the araBDA operon, upstream of which three other genes, araR (LacI-type transcriptional regulator), araE (L-arabinose transporter), and galM ( putative aldose 1-epimerase), were present in the opposite direction. Inactivation of the araA, araB, or araD gene eliminated growth on L-arabinose, and each of the gene products was functionally homologous to its Escherichia coli counterpart. Moreover, compared to the wild-type strain, an araE disruptant exhibited a > 80% decrease in the growth rate at a lower concentration of L-arabinose (3.6 g liter(-1)) but not at a higher concentration of L-arabinose ( 40 g liter(-1)). The expression of the araBDA operon and the araE gene was L-arabinose inducible and negatively regulated by the transcriptional regulator AraR. Disruption of araR eliminated the repression in the absence of L-arabinose. Expression of the regulon was not repressed by D-glucose, and simultaneous utilization of L-arabinose and D-glucose was observed in aerobically growing wild-type and araR deletion mutant cells. The regulatory mechanism of the L-arabinose regulon is, therefore, distinct from the carbon catabolite repression mechanism in other bacteria.
AMER SOC MICROBIOLOGY, 2009年06月, Applied and Environmental Microbiology, 75 (11), 3419 - 3429, 英語[査読有り]
研究論文（学術雑誌）
Corynebacterium glutamicum was metabolically engineered to broaden its substrate utilization range to include the pentose sugar L-arabinose, a product of the degradation of lignocellulosic biomass. The resultant CRA1 recombinant strain expressed the Escherichia coli genes araA, araB, and araD encoding L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase, respectively, under the control of a constitutive promoter. Unlike the wild-type strain, CRA1 was able to grow on mineral salts medium containing L-arabinose as the sole carbon and energy source. The three cloned genes were expressed to the same levels whether cells were cultured in the presence of D-glucose or L-arabinose. Under oxygen deprivation and with L-arabinose as the sole carbon and energy source, strain CRA1 carbon flow was redirected to produce up to 40, 37, and 11%, respectively, of the theoretical yields of succinic, lactic, and acetic acids. Using a sugar mixture containing 5% D-glucose and 1% L-arabinose under oxygen deprivation, CRA1 cells metabolized L-arabinose at a constant rate, resulting in combined organic acids yield based on the amount of sugar mixture consumed after D-glucose depletion (83%) that was comparable to that before D-glucose depletion (89%). Strain CRA1 is, therefore, able to utilize L-arabinose as a substrate for organic acid production even in the presence of D-glucose.
SPRINGER, 2008年01月, Applied Microbiology and Biotechnology, 77 (5), 1053 - 1062, 英語[査読有り]
研究論文（学術雑誌）
- 2007年10月, Applied and Environmental Microbiology, 73 (19), 6328, 英語
[査読有り]
研究論文（学術雑誌）
In cellulosic ethanol production, pretreatment of a biomass to facilitate enzymatic hydrolysis inevitably yields fermentation inhibitors such as organic acids, furans, and phenols. With representative inhibitors included in the medium at various concentrations, individually or in various combinations, ethanol production by Corynebacterium glutamicum R under growth-arrested conditions was investigated. In the presence of various inhibitors, the 62 to 100% ethanol productivity retained by the C. glutamicum R-dependent method far exceeded that retained by previously reported methods.
AMER SOC MICROBIOLOGY, 2007年04月, Applied and Environmental Microbiology, 73 (7), 2349 - 2353, 英語[査読有り]
研究論文（学術雑誌）
A three-step biohydrogen production process characterized by efficient anaerobic induction of the formate hydrogen lyase (FHL) of aerobically grown Escherichia coli was established. Using E. coli strain SR13 (fhlA(++), Delta hycA) at a cell density of 8.2 g/l medium in this process, a specific hydrogen productivity (28.0 +/- 5.0 mmol h(-1) g(-1) dry cell) of one order of magnitude lower than we previously reported was realized after 8 h of anaerobic incubation. The reduced productivity was attributed partly to the inhibitory effects of accumulated metabolites on FHL induction. To avoid this inhibition, strain SR14 (SR13 Delta ldhA Delta frdBC) was constructed and used to the effect that specific hydrogen productivity increased 1.3-fold to 37.4 +/- 6.9 mmol h(-1) g(-1). Furthermore, a maximum hydrogen production rate of 144.2 mmol h(-1) g(-1) was realized when a metabolite excretion system that achieved a dilution rate of 2.0 h(-1) was implemented. These results demonstrate that by avoiding anaerobic cultivation altogether, more economical harvesting of hydrogen-producing cells for use in our biohydrogen process was made possible.
SPRINGER, 2007年03月, Applied Microbiology and Biotechnology, 74 (4), 754 - 760, 英語[査読有り]
研究論文（学術雑誌）
We improved the hydrogen yield from glucose using a genetically modified Escherichia coli. E. coli strain SR15 (Delta ldhA, Delta frdBC), in which glucose metabolism was directed to pyruvate formate lyase (PFL), was constructed. The hydrogen yield of wild-type strain of 1.08 mol/mol glucose, was enhanced to 1.82 mol/mol glucose in strain SR15. This figure is greater than 90 % of the theoretical hydrogen yield of facultative anaerobes (2.0 mol/mol glucose). Moreover, the specific hydrogen production rate of strain SR15 (13.4 mmol h(-1) g(-1) dry cell) was 1.4-fold higher than that of wild-type strain. In addition, the volumetric hydrogen production rate increased using the process where cells behaved as an effective catalyst. At 94.3 g dry cell/l, a productivity of 793 mmol h(-1) l(-1) (20.2 l h(-1) l(-1) at 37 degrees C) was achieved using SR15. The reported productivity substantially surpasses that of conventional biological hydrogen production processes and can be a trigger for practical applications.
SPRINGER, 2006年11月, Applied Microbiology and Biotechnology, 73 (1), 67 - 72, 英語[査読有り]
研究論文（学術雑誌）
The aerobic microorganism Corynebacterium glutamicum was metabolically engineered to broaden its substrate utilization range to include the pentose sugar xylose, which is commonly found in agricultural residues and other lignocellulosic biomass. We demonstrated the functionality of the corynebacterial xylB gene encoding xylulokinase and constructed two recombinant C glutamicum strains capable of utilizing xylose by cloning the Escherichia coli gene xyl4 encoding xylose isomerase, either alone (strain CRX1) or in combination with the E. coli gene xylB (strain CRX2). These genes were provided on a high-copy-number plasmid and were under the control of the constitutive promoter trc derived from plasmid pTrc99A. Both recombinant strains were able to grow in mineral medium containing xylose as the sole carbon source, but strain CRX2 grew faster on xylose than strain CRX1. We previously reported the use of oxygen deprivation conditions to arrest cell replication in C. glutamicum and divert carbon source utilization towards product production rather than towards vegetative functions (M. Inui, S. Murakami, S. Okino, H. Kawaguchi, A. A. Vertes, and H. Yukawa, J. Mol. Microbiol. Biotechnol. 7:182-196, 2004). Under these conditions, strain CRX2 efficiently consumed xylose and produced predominantly lactic and succinic acids without growth. Moreover, in mineral medium containing a sugar mixture of 5% glucose and 2.5% xylose, oxygen-deprived strain CRX2 cells simultaneously consumed both sugars, demonstrating the absence of diauxic phenomena relative to the new xyl4-xylB construct, albeit glucose-mediated regulation still exerted a measurable influence on xylose consumption kinetics.
AMER SOC MICROBIOLOGY, 2006年05月, Applied and Environmental Microbiology, 72 (5), 3418 - 3428, 英語[査読有り]
研究論文（学術雑誌）
Genetic recombination of Escherichia coli in conjunction with process manipulation was employed to elevate the efficiency of hydrogen production in the resultant strain SR13 2 orders of magnitude above that of conventional methods. The formate hydrogen lyase (FHL) -overexpressing strain SR13 was constructed by combining FHL repressor (hycA) inactivation with FHL activator (fhlA) overexpression. Transcription of large-subunit formate dehydrogenase,fdhF, and large-subunit hydrogenase, hycE, in strain SR13 increased 6.5-and 7.0-fold, respectively, compared to the wild-type strain. On its own, this genetic modification effectively resulted in a 2.8-fold- increase in hydrogen productivity of SR13 compared to the wild-type strain. Further enhancement of productivity was attained by using a novel method involving the induction of the FHL complex with high-cell-density filling of a reactor under anaerobic conditions. Continuous hydrogen production was achieved by maintaining the reactor concentration of the substrate (free formic acid) under 25 mM. An initial productivity of 23.6 g hydrogen h(-1) liter(-1) (300 liters h(-1) liter(-1) at 37 degrees C) was achieved using strain SR13 at a cell density of 93 g (dry weight) cells/liter. The hydrogen productivity reported in this work has great potential for practical application.
AMER SOC MICROBIOLOGY, 2005年11月, Applied and Environmental Microbiology, 71 (11), 6762 - 6768, 英語[査読有り]
研究論文（学術雑誌）
The central metabolic pathway of Corynebacterium glutamicum was engineered to produce ethanol. A recombinant strain which expressed the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) was constructed. Both genes placed under the control of the C. glutamicum ldhA promoter were expressed at high levels in C. glutamicum, resulting, under oxygen-deprivation conditions, in a significant yield of ethanol from glucose in a process characterized by the absence of cellular growth. Addition of pyruvate in trace amounts to the reaction mixture induced a 2-fold increase in the ethanol production rate. A similar effect was observed when acetaldehyde was added. Disruption of the lactate dehydrogenase ( ldhA) gene led to a 3-fold higher ethanol yield than wild type, with no lactate production. Moreover, inactivation of the phosphoenolpyruvate carboxylase (ppc) and ldhA genes revealed a significant amount of ethanol production and a dramatic decrease in succinate without any lactate production, when pyruvate was added. Since the reaction occurred in the absence of cell growth, the ethanol volumetric productivity increased in proportion to cell density of ethanologenic C. glutamicum in a process under oxygen-deprivation conditions. These observations corroborate the view that intracellular NADH concentrations in C. glutamicum are correlated to oxygen-deprived metabolic flows. Copyright (C) 2004 S. Karger AG, Basel.
KARGER, 2004年, Journal of Molecular Microbiology and Biotechnology, 8 (4), 243 - 254, 英語[査読有り]
研究論文（学術雑誌）
Lactate and succinate were produced from glucose by Corynebacterium glutamicum under oxygen deprivation conditions without growth. Addition of bicarbonate to the reaction mixture led not only to a 3.6-fold increase in succinate production rate, but also to a 2.3- and 2.5-fold increase, respectively, of the rates of lactate production and glucose consumption, compared to the control. Furthermore, when small amounts of pyruvate were added to the reaction mixture, acid production rates and the glucose consumption rate were multiplied by a factor ranging from 2 to 3. These phenomena were paralleled by an increase in the NAD(+)/NADH ratio, thus corroborating the view that the efficient regeneration of NAD+ could be triggered by the addition of either bicarbonate or pyruvate. To investigate the global metabolism of corynebacteria under oxygen deprivation conditions, we engineered several strains where the genes coding for key metabolic enzymes had been inactivated by gene disruption and replacement. A lactate dehydrogenase (LDH)-deficient mutant was not able to produce lactate, suggesting this enzyme has no other isozyme. Although a pyruvate carboxylase (pyc) mutant exhibited similar behavior to that of the wild type, phosphoenolpyruvate carboxylase (ppc) mutants were characterized by a dramatic decrease in succinate production, which was concomitant to decreased lactate production and glucose consumption rates. This set of observations corroborates the view that in coryneform bacteria under oxygen deprivation conditions the major anaplerotic reaction is driven by the ppc gene product rather than by the pyc gene product. Moreover, intracellular NADH concentrations in C. glutamicum were observed to correlate to oxygen-deprived metabolic flows. Copyright (C) 2004 S. Karger AG, Basel.
KARGER, 2004年, Journal of Molecular Microbiology and Biotechnology, 7 (4), 182 - 196, 英語[査読有り]
研究論文（学術雑誌）
We have established a system for hydrogen (H-2) production from algal starch via lactic acid using a mixed culture of a lactic acid bacterium, Lactobacillus amylovorus, and a photosynthetic bacterium, Rhodobium marinum A-501. We found that the H, production from lactate was stimulated in the presence of algal extract, which was obtained from algal biomass homogenate used as a substrate in the system by removing settleable solids including starch. To analyze the stimulating effect of algal extract on H-2 production, we developed a kinetic model for H-2 production by R. marinum A-501. The model revealed that approximately 20% of lactate was consumed for cell mass production, and the remaining portion was a source of reducing power to drive hydrogen production or other cellular processes. In the presence of algal extract, the model indicated that the conversion efficiency from lactate to the reducing power increased from 0.56 to 0.80 and nitrogenase activity increased up to twofold, resulting in the increase in yield of hydrogen from lactate from 29% to 48%. These results suggest that algal extract can attenuate the limitation process in lactate catabolism by which the supplementation of reducing power to drive H-2 production was suppressed.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2002年07月, Journal of Bioscience and Bioengineering, 94 (1), 62 - 69, 英語[査読有り]
研究論文（学術雑誌）
- H2 production from algal biomass by a mixed culture of Rhodobium marinum A-501 and Lactobacillus amylovorus
To produce hydrogen from starch accumulated in an algal biomass, we used a mixed culture of the lactic acid bacterium, Lactobacillus amylovorus, and the photosynthetic bacterium, Rhodobium marinum A-501. In this system L. amylovorus, which possesses amylase activity, utilized algal starch for lactic acid production, and R. marinum A-501 produced hydrogen in the presence of light using lactic acid as an electron donor. Algal starch accumulated in the marine green alga Dunaliella tertiolecta, and the freshwater green alga Chlamydomonas reinhardtii, was more suitable for lactic acid fermentation by L. amylovorus than an authentic starch sample. Consequently, the yields of hydrogen obtained from starch contained in D. tertiolecta and C. reinhardtii were 61% and 52%, respectively, in the mixed culture of L. amylovorus and R. marinum A-501. These values were markedly superior to those obtained using a mixed culture of Vibrio fluvialis T-522 and R. marinum A-501 described previously. The yield and production rate of hydrogen by R. marinum A-501 from the lactic acid fermentates were higher than from authentic Lactic acid, suggesting that the fermentates contain a factor(s) which promotes H-2 production by this bacterium.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2001年03月, Journal of Bioscience and Bioengineering, 91 (3), 277 - 282, 英語[査読有り]
研究論文（学術雑誌）
The bacterial community designated BC1, which originates from night soil treatment sludge, exhibited a strong ability to produce H-2 from raw starch in the light in the presence of 3% NaCl. Three halophilic or halotolerant bacterial species, Vibrio fluvialis, Rhodobium marinum, and Proteus vulgaris, were isolated from BC1 and identified. The level of H-2 production from starch by coculture of V. fluvialis and R. marinum was nearly equal to that by BC1, indicating that these two strains play roles in starch degradation and H-2 production from the degraded products in BC1, respectively. Acetic acid and ethanol, which were detected as the major products of degradation of starch by V. fluvialis in pure culture, seemed to be mainly utilized for H-2 production by R. marinum in BC1 and the coculture. However, R. marinum in pure culture could not produce H-2 from a synthetic medium containing acetic acid and ethanol, suggesting that V. fluvialis supplied not only substrates but also some unknown factors capable of inducing H-2 production from these substrates by R. marinum. A study using the starch-rich microalgae, Chlamydomonas reinhardtii and Dunaliella tertiolecta, demonstrated that the above coculture could be applied to the production of H-2 at high yield from raw starch in an algal biomass.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 1999年07月, Journal of Bioscience and Bioengineering, 88 (1), 72 - 77, 英語[査読有り]
研究論文（学術雑誌）
- Microbiology Research Foundation, 2022年07月12日, The Journal of General and Applied Microbiology, in press, 英語
[査読有り]
研究論文（学術雑誌）
MISC
- 2019年, 日本生物工学会大会講演要旨集, 71stセルロース系バイオマスを原料とするカフェ酸発酵のためのゲノム編集による大腸菌代謝改変の作製と培養法の検討
- 2017年03月, ケミカルエンジニヤリング, 62 (3), 13 - 20, 日本語リグノセルロース系バイオマスからのバイオモノマー発酵生産
[査読有り]
記事・総説・解説・論説等（学術雑誌）
- 2016年10月14日, リグニン討論会講演集, 61st, 180‐181, 日本語疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発
- 2016年03月05日, 日本農芸化学会大会講演要旨集(Web), 2016, 3A030 (WEB ONLY), 日本語アルコールの添加が希硫酸前処理の効率に与える影響の解析
- 2016年02月, 慶応義塾大学出版会, 87 - 104, 日本語スーパーバイオマス：第6章 バイオリファイナリー
記事・総説・解説・論説等（学術雑誌）
- 2016年, 日本農芸化学会大会講演要旨集(Web), 2016紙パルプの同時糖化発酵によるカフェ酸の微生物生産
- 2015年03月09日, 日本植物生理学会年会要旨集, 56th, 284, 日本語2次元NMRを用いた希硫酸前処理後固体画分の組成解析
- 2014年07月, ケミカルエンジニヤリング, 59 (7), 506 - 515, 日本語バイオマスからの微生物によるバイオモノマー生産
記事・総説・解説・論説等（学術雑誌）
- 日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 206 - 206, 日本語3P-045 バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響(オミクス解析,一般講演)
- 2013年05月30日, 環境バイオテクノロジー学会大会プログラム講演要旨集, 2013, 34, 日本語Rhodococcus opacus B‐4の溶媒耐性に関するトランスクリプトーム解析
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 47 - 47, 日本語1P-118 放線菌による芳香族化合物ホモゲンチジン酸の生産(一般講演(発酵生理学,発酵工学))
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 44 - 44, 日本語1P-105 同時糖化発酵によるセルロースからのフェニル乳酸生産(一般講演(発酵生理学,発酵工学))
- 2012年07月, 石油技術協会誌, 77 (4), 289, 日本語好アルカリ性細菌を用いたSAGD法プロセス水に含まれる水溶性有機物の微生物分解
- 2012年06月25日, 環境バイオテクノロジー学会大会プログラム講演要旨集, 2012, 33, 日本語Rhodococcus opacus B‐4の生体異物代謝における排出タンパク質の機能解析
- 2012年03月05日, 日本農芸化学会大会講演要旨集(Web), 2012, 3C02P08 (WEB ONLY), 日本語オイルサンド生産における高アルカリ性プロセス水環境に内在する微生物群の有機化合物分解特性と菌叢解析
- AMER CHEMICAL SOC, 2012年03月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 243, 英語Exoelectrogenic ability of thermophilic microorganisms from various environments
研究発表ペーパー・要旨（国際会議）
- 2011年10月08日, 日本微生物生態学会講演要旨集, 27th, 102, 英語Analysis of microbial activity in thermophilic bioelectrochemical reactors
- 2011年10月08日, 日本微生物生態学会講演要旨集, 27th, 64, 日本語メタン生成的条件下における電気化学的メタン生成活性を持つ微生物群の解析
- 2011年10月08日, 日本微生物生態学会講演要旨集, 27th, 64, 日本語メタン菌による電気化学的メタン生成反応の解析と数値モデル化
- 公益社団法人日本生物工学会, 2011年08月25日, 日本生物工学会大会講演要旨集, 63, 日本語オイルサンド生産におけるアルカリ性プロセス水に対する微生物浄化法の応用と菌相解析(環境工学・廃水処理技術/環境浄化・修復・保全技術,一般講演)
- 2011年07月, 石油技術協会誌, 76 (4), 362, 日本語SAGD法におけるプロセス水リサイクルに向けた微生物浄化反応の解析
- 2011年06月20日, 環境バイオテクノロジー学会大会プログラム講演要旨集, 44th, 24, 日本語Rhodococcus opacusを用いた水‐油2相反応系における微生物脱硫促進効果の解析
- 2011年03月05日, 日本農芸化学会大会講演要旨集, 2011, 7, 日本語重質油生産プラントにおけるアルカリ性プロセス水処理技術としての微生物浄化法の利用と菌相解析
- 2010年11月25日, 石油・石油化学討論会講演要旨, 40th, 146 - 147, 日本語SAGD法におけるプロセス水リサイクルに向けた微生物浄化技術利用に関する検討
- 2010年11月23日, 日本微生物生態学会講演要旨集, 26th, 83, 英語Study to enhance methanogenesis by subsurface microorganisms within petroleum reservoirs
- 2010年11月23日, 日本微生物生態学会講演要旨集, 26th, 193, 日本語微生物を用いた電気化学反応の環境技術への応用
- 2010年11月23日, 日本微生物生態学会講演要旨集, 26th, 148, 日本語メタン菌によるメタン生成のモデル化に関する研究
- 2010年07月, 石油技術協会誌, 75 (4), 319, 日本語SAGD法におけるプロセス水の化学的分析と微生物浄化技術利用の検証
- 2010年07月, 石油技術協会誌, 75 (4), 319, 日本語地下環境での利用に向けた微生物燃料電池の多孔質媒体内挙動解析
- 2010年07月, 石油技術協会誌, 75 (4), 319 - 320, 日本語枯渇油田における微生物利用メタン生成活性手法の検討
- 2010年03月05日, 日本農芸化学会大会講演要旨集, 2010, 131, 日本語有機溶媒耐性菌Rhodococcus opacus B‐4の機能改変による重質油の選択的脱硫触媒としての応用
- 2010年, 環境バイオテクノロジー学会大会プログラム講演要旨集, 40th, 31, 日本語遺伝子組換えRhodococcus opacus B‐4におけるDibenzothiophene代謝に関与する遺伝子群の発現解析
オイルサンドの油層内回収法（SAGD法）における生産水の環境調和型リサイクル技術の開発を目的に、プロセス水の化学分析と微生物浄化法の利用を検討した。JAPEX現地法人がカナダで操業するSAGDプラントからプロセス水を採取し、各工程水の化学組成を比較すると共に、含有水溶性有機物の同定と定量解析を行った。また、各種プロセス水に微生物を接種したサンプルについて水溶性有機物濃度の低下を測定し、生物的浄化技術の適合性を検証した。
The Japan Petroleum Institute, 2010年, 石油学会 年会・秋季大会講演要旨集, 2010 (0), 96 - 96, 日本語- 2009年07月, 石油技術協会誌, 74 (4), 368, 日本語地中貯留二酸化炭素の再資源化を想定した微生物混合培養によるメタン生成プロセスへの過剰CO2の影響評価
- 2009年03月05日, 日本農芸化学会大会講演要旨集, 2009, 329, 日本語原油浸潤土壌からのdibenzothiopheneおよびcarbazole分解性菌の単離・同定
- 2007年12月, 生物工学会誌, 85 (12), 550, 日本語地下微生物圏へのアプローチ ―石油と微生物の関わり―
[査読有り]
記事・総説・解説・論説等（学術雑誌）
- AMER SOC MICROBIOLOGY, 2007年10月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73 (19), 6328 - 6328, 英語
その他
- AMER CHEMICAL SOC, 2007年08月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 234, 英語BIOT 266-Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum
研究発表ペーパー・要旨（国際会議）
- 2006年04月, Ceramics Japan, 41, 312, 日本語バイオ法水素生産の新展開
記事・総説・解説・論説等（学術雑誌）
- AMER SOC MICROBIOLOGY, 2006年02月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72 (2), 1716 - 1716, 英語
その他
- 日本生物工学会, 2006年, 日本生物工学会大会講演要旨集, 18, 日本語コリネ型細菌を用いたバイオエタノール生産プロセスの構築に関する基礎的検討(生物化学工学,一般講演)
- 2006年, 電気評論, 91 (10), 62 - 63, 日本語バイオ水素研究の展開
記事・総説・解説・論説等（学術雑誌）
- 2006年, The Journal of Fuel Cell Technology, 5 (4), 83 - 85, 英語H2 production from formic acid using an innovative bioprocess
記事・総説・解説・論説等（学術雑誌）
- 燃料電池開発情報センター, 2006年, 燃料電池, 5 (4), 83 - 85, 日本語技術情報 バイオマスから水素生産--新規バイオプロセスの利用
- 2004年03月, 日本農芸化学会大会講演要旨集, 2004, 180, 日本語組換えコリネ型細菌を利用した木材からの高効率エタノール生産:発酵阻害物質の影響について
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 化学工業社, 2017年03月, ケミカルエンジニヤリング = Chemical engineering, 62 (3), 169 - 176, 日本語リグノセルロース系バイオマスからのバイオモノマー発酵生産 (特集 バイオマス利活用のイノベーション)
- 2012年11月05日, 化学工学 = Chemical engineering, 76 (11), 722 - 722, 日本語神戸大学大学院工学研究科応用化学専攻 生物化学工学研究室
- 日本生物工学会, 2006年, 日本生物工学会大会講演要旨集, 18, 211 - 211, 日本語1L09-5 嫌気代謝経路改変大腸菌によるグルコースからの高収率水素生成(生物化学工学,一般講演)
書籍等出版物
- 共著, シーエムシー出版, 2005年09月, 日本語エコバイオエネルギーの最前線：ゼロエミッション型社会を目指して, ６．RITEバイオプロセスによるエタノール製造
学術書
- 共著, コロナ社, 2005年06月, 日本語生物工学ハンドブック, Ⅱ．生物工学技術の実際「バイオマス資源からのエタノール生産」
学術書
- 共著, シーエムシー出版, 2004年08月, 日本語CO2固定化・削減・有効利用の最新技術：地球温暖化対策関連技術, バイオ水素法の新展開
学術書
- 共著, Pergamon Press, 2001年, 英語Biohydrogen II, Hydrogen photoproduction from starch in algal biomass
学術書
講演・口頭発表等
- 第73回日本生物工学会大会, 2121年10月28日, 日本語酸素制限による遺伝子組換えコリネ型細菌を用いた3-amino-4-hydroxybenzoic acid 発酵の促進作用
ポスター発表
- JST未来社会創造事業「地球規模課題である低炭素社会の実現領域」本格研究『雑種強勢の原理解明によるバイオマス技術革新』キックオフシンポジウム, 2021年12月02日, 日本語ソルガムの利用促進のためのカタログ化と微生物によるものづくり
シンポジウム・ワークショップパネル（指名）
- The 26th Symposium of Young Asian Biological Engineers’ Community (YABEC 2021), 2021年11月19日, 英語Microbial monomer production from lignocellulosic biomass for bioplastic synthesis
口頭発表（一般）
- 第12回 ソルガムワークショップ, 2021年11月17日, 日本語微生物発酵によるソルガムバイオマスからのものづくり
口頭発表（一般）
- 2021年度 第12回グリーンソサエティーセミナー, 2021年11月02日, 日本語微生物を活用した循環型社会の構築：カーボンニュートラルな植物資源からつくる新しいバイオプラスチック
[招待有り]
公開講演，セミナー，チュートリアル，講習，講義等
- Metabolic Engineering 14, 2021年07月13日, 英語Metabolome analysis-based design of a metabolic pathway in Corynebacterium glutamicum for 3-amino-4-hydroxybenzoic acid production under oxygen limitation
ポスター発表
- World Microbe Forum 2021, 2021年06月21日, 英語Amino acid metabolism of Corynebacterium glutamicum under oxygen limitation
ポスター発表
- 日本農芸化学会 2021年度仙台大会, 2021年03月21日, 日本語遺伝子組換え大腸菌によるフェニル乳酸発酵をモデルとする発酵阻害メカニズムの解明
ポスター発表
- 日本育種学会 第139回講演会, 2021年03月20日, 日本語ソルガムF1品種「天高」の雑種強勢は優性説によって概ね必要十分に説明される
口頭発表（一般）
- 第6回デザイン生命工学研究会, 2021年03月05日, 日本語溶存酸素濃度が γ-アミノ酸 3-amino-4-hydroxybenzoic acid を生産する組換えコリネ型細菌の代謝プロファイルに与える影響
口頭発表（一般）
- 日本農芸化学会関西支部第513回講演会, 2020年11月28日, 日本語菌糸分散型麹菌のタンパク質高生産性と加速された糖代謝
口頭発表（一般）
- JAIST World Conference 2020―International Symposium for Innovative Sustainable Materials & The 7th International Symposium for Green-Innovation Polymers (GRIP2020), 2020年11月09日, 英語Microbial production of aromatic compounds applied to synthesize bioplastics from plant biomass
[招待有り]
口頭発表（招待・特別）
- 北勢バイオコミュニティ研究会キックオフセミナー, 2020年11月06日, 日本語微生物を活用したバイオリファイナリー研究：植物からつくるバイオプラスチック
[招待有り]
口頭発表（招待・特別）
- 2020年度グラム陽性細菌ゲノム機能会議, 2020年09月15日, 日本語溶存酸素濃度の制御による組換えコリネ型細菌を用いたグルコースからの3-amino-4-hydroxybenzoic acid発酵の促進作用機構解析
口頭発表（一般）
- ASM Microbe Online, 2020年07月22日, 英語Caffeic acid production from diluted acid-pretreated sorghum bagasse by metabolically engineered Escherichia coli
ポスター発表
- 日本農芸化学会2020年度大会, 2020年03月27日, 日本語メタボローム解析が明らかにする低酸素濃度におけるコリネ型細菌の芳香族化合物代謝促進機構と代謝改変によるソルガム搾汁液からの物質生産
口頭発表（一般）
- 第10回 ソルガムワークショップ, 2019年11月19日, 日本語, 国内会議ソルガムの酵素糖化効率品種間差を決定する物理化学特性の探索
シンポジウム・ワークショップパネル（公募）
- 日本農芸化学会 関西・中部支部 2019年度合同神戸大会, 2019年09月22日, 日本語, 国内会議ソルガムバガスの酵素糖化効率支配因子の探索
[招待有り]
口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月17日, 日本語, 岡山大学, 国内会議酸素をスイッチとしたコリネ型細菌中央代謝経路の切り換え
口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月17日, 日本語, 岡山大学, 国内会議セルロース系バイオマスを原料とするカフェ酸発酵のためのゲノム編集による大腸菌代謝改変の作 製と培養法の検討
口頭発表（一般）
- みえバイオリファイナリー研究会公開セミナー, 2019年07月22日, 日本語, 三重大学, 国内会議微生物を活用してバイオマスからバイオプラスチックを作る
[招待有り]
公開講演，セミナー，チュートリアル，講習，講義等
- 生物工学若手研究者の集い 夏のセミナー2019, 2019年07月20日, 英語, 国内会議ソルガムを原料とするバイオリファイナリー：糖化液からのカフェ酸発酵
ポスター発表
- 生物工学若手研究者の集い 夏のセミナー2019, 2019年07月, 日本語, 国内会議バイオマス植物を原料とする遺伝子組換え大腸菌によるカフェ酸発酵
公開講演，セミナー，チュートリアル，講習，講義等
- ASM Microbe 2019, 2019年06月23日, 英語, 国際会議Metabolic Engineering of Escherichia coli for Enhanced Production of 4-Aminophenylalanine
ポスター発表
- 日本農芸化学会2019年度大会, 2019年03月23日, 日本語, 東京農業大学 世田谷キャンパス, 国内会議4-アミノフェニルアラニン発酵における組換え大腸菌の代 謝改変
ポスター発表
- 2019 Advanced Biosynthesis and Biorefinery Workshop, 2019年03月, 英語, Xi’an Jiaotong University, 国際会議Development of microbial cell factories for production of aromatic chemicals and derivatives
[招待有り]
口頭発表（基調）
- 日本農芸化学会 関西支部第507回講演会, 2019年02月, 日本語, 国内会議溶存酸素濃度がシキミ酸誘導体の発酵生産に与える影響
- The 10th International Symposium of Innovative BioProduction Kobe, 2019年01月25日, 英語, 国際会議Bidirectional switching of carbon flow between glycolysis and pentose phosphate pathway by oxygen level in Corynebacterium glutamicum
ポスター発表
- The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), 2018年11月, 英語, National Taiwan University, 国際会議Caffeic acid production from lignocellulosic biomass by metabolically engineered Eshrichaia coli
口頭発表（一般）
- 第70回日本生物工学会大会, 2018年09月, 日本語, 日本生物工学会, 関西大学 千里山キャンパス, 国内会議二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析
ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議組換えコリネ型細菌を用いた3-アミノ-4-ヒドロキシ安息香酸の発酵生産における酸素制限による 促進作用
ポスター発表
- ASM Microbe 2018, 2018年06月, 英語, 国際会議Phenyllactic Acid Productionby Recombinant Escherichia coli fromSorghum Bagasse: Fermentation Inhibitionby the Enzymatic Hydrolysate
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議組換え大腸菌の代謝改変による4-アミノフェニルアラニンの発酵生産
ポスター発表
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議酸素をスイッチとしたコリネ型細菌中央代謝経路の切り替え
ポスター発表
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議リグノセルロース系バイオマスを包括的に利用したコリネ型細菌によるバイオモノマー生産
ポスター発表
- 日本化学会第98春季年会2018, 2018年03月, 日本語, 日本化学会, 船橋市, 国内会議リグノセルロースナノファイバー表面のリグニン分布解析手法の開発
ポスター発表
- 第13回バイオマス科学会議, 2018年01月, 英語, 日本エネルギー学会, 仙台市, 国際会議同時糖化発酵プロセスを利用した紙パルプを原料とするカフェ酸の生産
口頭発表（一般）
- 第7回CSJ化学フェスタ2017, 2017年10月, 日本語, 日本化学会, 東京都, 国内会議リグニン残量を制御したセルロースから得られた各種ナノファイバーの構造と物性
ポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議酸素をスイッチとした二つの樹脂原料の個別生産
ポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議高度膜分離技術によるソルガム搾汁液からのスクロース分離および連続的エタノール生産
口頭発表（一般）
- 第63回高分子研究発表会(神戸), 2017年07月, 日本語, 高分子学会関西支部, 神戸市, 国内会議リグニン量を制御したセルロースナノファイバーの構造と物性
口頭発表（一般）
- 平成29年度繊維学会年次大会, 2017年06月, 日本語, 繊維学会, 東京都, 国内会議リグニン含有量を異にするセルロースから得られた各種ナノファイバーの構造と物性
ポスター発表
- 日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議組換え大腸菌によるグルコースを原料とする4-アミノ 桂皮酸の直接発酵生産
口頭発表（一般）
- 第61回リグニン討論会, 2016年10月, 日本語, リグニン討論会実行委員会, 宇治市, 国内会議疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発
口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析
口頭発表（一般）
- 化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議アルコールの添加が希硫酸前処理の効率に与える影響の解析
口頭発表（一般）
- 第5回JACI GSCシンポジウム, 2016年06月, 日本語, 新化学技術推進協会, 神戸市, 国内会議アルコールの添加が希硫酸前処理の効率に与える影響の解析
ポスター発表
- Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議Caffeic Acid Production By Simultaneous Saccharification and Fermentation of Kraft Pulp Using Recombinant Escherichia coli
口頭発表（一般）
- 日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議紙パルプの同時糖化発酵によるカフェ酸の微生物生産
口頭発表（一般）
- 日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議アルコールの添加物が希硫酸前処理の効率に与える影響の解析
口頭発表（一般）
- 第11回バイオマス科学会議, 2016年01月, 日本語, 国際会議リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産
口頭発表（一般）
- 神戸大学若手フロンティア2015, 2015年12月, 日本語, 神戸大学, 国内会議稲わらのバイオリファイナリーに向けた特性の解析と多様性の評価
ポスター発表
- 日本農芸化学会関西支部第492例会, 2015年12月, 日本語, 神戸大学, 国内会議バイオリファイナリー利用に向けた稲わらの希硫酸前処理後グルコース含量の自然変異
口頭発表（一般）
- 日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議膜濃縮したソルガム搾汁液からのエタノール発酵
口頭発表（一般）
- 日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議組換えコリネ型細菌による3-hydroxy-4-aminobenzoic acid発酵におけるソルガム搾汁液の促進作用
口頭発表（一般）
- 第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響
ポスター発表
- 生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議リグノセルロース系バイオマスからのバイオ芳香族化合物の生産
ポスター発表
- 日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議同時糖化発酵による希硫酸前処理バイオマスからのフェニル乳酸生産
口頭発表（一般）
- 第55回日本植物生理学会年会, 2014年03月, 日本語, 富山, 国内会議バイオマス評価法の開発
ポスター発表
- 第55回日本植物生理学会年会, 2014年03月, 日本語, 日本植物生理学会, 富山市, 国内会議バイオマス特性評価法とバイオリファイナリーへの適用
口頭発表（一般）
- Asia BioHyLink 2013meeting, 2013年11月, 英語, Osaka, Japan, 国際会議Microbial water treatment to remove dissolved organic compounds in reused process water for steam-assisted gravity drainage oil sands extraction
[招待有り]
口頭発表（招待・特別）
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議放線菌による芳香族化合物ホモゲンチジン酸の生産
ポスター発表
- 日本接着学会関西支部 第9回若手の会, 2013年09月, 日本語, 神戸, 国内会議微生物発酵によるバイオマス原料からのモノマー生産
[招待有り]
口頭発表（招待・特別）
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議同時糖化発酵によるセルロースからのフェニル乳酸生産
ポスター発表
- Biobased Economy Summer School, 2013年08月, 英語, Ghent, Belgium, 国際会議Bio-based chemical production from cellulosic feedstock
ポスター発表
- 環境バイオテクノロジー学会 2013年度大会, 2013年05月, 日本語, 北九州, 国内会議Rhodococcus opacus B-4の溶媒耐性に関するトランスクリプトーム解析
口頭発表（一般）
- 11th International Conference on Greenhouse Gas Control Technologies (GHGT-11), 2012年12月, 英語, Kyoto, Japan, 国際会議Identification of new microbial mediators for electromethanogenic reduction of geologically-stored carbon dioxide
口頭発表（一般）
- American Chemical Society 243th National Meeting & Exposition, 2012年08月, 英語, CA, USA, 国際会議Exoelectrogenic ability of thermophilic microorganisms from various environments
口頭発表（一般）
- 環境バイオテクノロジー学会 2012年度大会, 2012年06月, 日本語, 京都, 国内会議Rhodococcus opacus B-4 の生体異物代謝における排出タンパク質の機能解析
口頭発表（一般）
- 日本農芸化学会 2012年度大会, 2012年03月, 日本語, 京都, 国内会議オイルサンド生産における高アルカリ性プロセス水環境に内在する微生物群の有機化合物分解特性と菌叢解析
口頭発表（一般）
- 日本微生物生態学会第27回大会, 2011年10月, 日本語, 国内会議メタン生成的条件下における電気化学的メタン生成活性を持つ微生物群の解析
口頭発表（一般）
- 日本微生物生態学会第27回大会, 2011年10月, 日本語, 国内会議メタン菌による電気化学的メタン生成反応の解析と数値モデル化
口頭発表（一般）
- 日本微生物生態学会第27回大会, 2011年10月, 英語, 京都, 国内会議Analysis of microbial activity in thermophilic bioelectrochemical reactors
ポスター発表
- 日本生物工学会 第63回大会, 2011年09月, 日本語, 国内会議オイルサンド生産におけるアルカリ性プロセス水に対する微生物浄化法の応用と菌相解析
口頭発表（一般）
- 有機溶媒耐性微生物利用技術研究部会 第２回シンポジウム, 2011年07月, 日本語, 国内会議Rhodococcus opacus を用いた微生物脱硫触媒の開発
[招待有り]
口頭発表（招待・特別）
- 環境バイオテクノロジー学会 2011年度大会, 2011年06月, 日本語, 国内会議Rhodococcus opacus B-4を用いた水－油２相反応系における微生物脱硫促進効果の解析
口頭発表（一般）
- 111th General Meeting American Society for Microbiology, 2011年05月, 英語, LA, USA, 国際会議Analysis of microorganisms in the process water used for unconventional oil production: Toward microbial water treatment in oil sands plants
ポスター発表
- 日本農芸化学会 2011年度大会, 2011年, 日本語, 国内会議重質油生産プラントにおけるアルカリ性プロセス水処理技術としての微生物浄化法の利用と菌相解析
口頭発表（一般）
- 平成23年度 石油技術協会 春季講演会, 2011年, 日本語, 国内会議SAGD法におけるプロセス水リサイクルに向けた微生物浄化反応の解析
口頭発表（一般）
- 日本農芸化学会 2010年度大会, 2010年, 日本語, 国内会議有機溶媒耐性菌Rhodococcus opacus B-4の機能改変による重質油の選択的脱硫触媒としての応用
口頭発表（一般）
- 日本微生物生態学会 第26回大会, 2010年, 日本語, 国内会議微生物を用いた電気化学反応の環境技術への応用
口頭発表（一般）
- 日本地球惑星科学連合 2010年大会, 2010年, 日本語, 国内会議地中貯留二酸化炭素の再資源化を想定したメタン生成バイオプロセスへの油層水成分の影響評価
口頭発表（一般）
- 第2回メタンハイドレート総合シンポジウム, 2010年, 日本語, 国内会議地下油田内微生物によるメタン生産
口頭発表（一般）
- 平成22年度 石油技術協会 春季講演会, 2010年, 日本語, 国内会議地下環境での利用に向けた微生物燃料電池の多孔質媒体内挙動解析
口頭発表（一般）
- 平成22年度 石油技術協会 春季講演会, 2010年, 日本語, 国内会議枯渇油田における微生物利用メタン生成活性手法の検討
口頭発表（一般）
- 平成22年度 石油技術協会 春季講演会, 2010年, 日本語, 国内会議枯渇油田における微生物を利用した原油分解メタン生成に関する研究
口頭発表（一般）
- 環境バイオテクノロジー学会 2010年度大会, 2010年, 日本語, 国内会議遺伝子組換えRhodococcus opacus B-4 におけるDibenzothiophene 代謝に関与する遺伝子群の発現解析
口頭発表（一般）
- 日本微生物生態学会 第26回大会, 2010年, 日本語, 国内会議メタン菌によるメタン生成のモデル化に関する研究
ポスター発表
- 日本微生物生態学会 第26回大会, 2010年, 英語, 国内会議Study to enhance methanogenesis by subsurface microorganisms within petroleum reservoirs
口頭発表（招待・特別）
- 石油学会 第40回石油・石油化学討論会, 2010年, 日本語, 国内会議SAGD法におけるプロセス水リサイクルに向けた微生物浄化技術利用に関する検討
口頭発表（一般）
- 平成22年度 石油技術協会 春季講演会, 2010年, 日本語, 国内会議SAGD法におけるプロセス水の化学的分析と微生物浄化技術利用の検証
口頭発表（一般）
- Abu Dhabi International Petroleum Exhibition & Conference, 2010年, 英語, Abu Dhabi, UAE, 国際会議Research for microbial conversion of residual oil into methane in depleted oil fields to develop new EOR process
ポスター発表
- 日本微生物生態学会 第25回大会, 2009年, 日本語, 国内会議天然ガス de novo 生産に向けた地下微生物群の研究
ポスター発表
- 平成21年度 石油技術協会 春季大会, 2009年, 日本語, 国内会議地中貯留二酸化炭素の再資源化を想定した微生物混合培養によるメタン生成プロセスへの過剰CO2の影響評価
口頭発表（一般）
- 日本地球惑星科学連合 2009年大会, 2009年, 日本語, 国内会議地中貯留二酸化炭素の再資源化を想定したメタン生成バイオプロセスの適応性評価
口頭発表（一般）
- 日本微生物生態学会 第25回大会, 2009年, 日本語, 国内会議地下微生物圏の二酸化炭素への応答
口頭発表（一般）
- 日本農芸化学会 2009年度大会, 2009年, 日本語, 国内会議原油浸潤土壌からのdibenzothiopheneおよびcarbazole分解性菌の単離・同定
ポスター発表
- 日本農芸化学会 2009年度大会, 2009年, 日本語, 国内会議L-Arabinose資化性コリネ型細菌の単離および代謝遺伝子の機能解析
ポスター発表
- 平成20年度 石油技術協会 春季大会, 2008年, 日本語, 国内会議地中貯留二酸化炭素のメタン変換を想定した微生物混合培養に関する研究
口頭発表（一般）
- 平成20年度 石油技術協会 春季大会, 2008年, 日本語, 国内会議多孔質媒体内流動における微生物輸送に及ぼす媒体特性の影響
口頭発表（一般）
- 平成20年度 石油技術協会 春季大会, 2008年, 日本語, 国内会議多孔質環境が微生物の吸着並びに代謝に与える影響の評価
口頭発表（一般）
- 日本農芸化学会 2008年度大会, 2008年, 日本語, 国内会議組換えコリネ型細菌によるソフトバイオマス由来混合糖の同時利用
口頭発表（一般）
- 平成20年度 石油技術協会 春季大会, 2008年, 日本語, 国内会議メタン生成におけるH2-CO2比の影響評価と細胞内エネルギーレベルとしてのATP測定
ポスター発表
- 7th International Symposium for Subsurface Microbiology, 2008年, 英語, Shizuoka, Japan, 国際会議Development of microbial processes capable of converting stored CO2 into methane for recovering energy in depleted oil reservoir
ポスター発表
- 日本農芸化学会 2007年度大会, 2007年, 日本語, 国内会議高密度菌体による水素生成プロセスのためのFHLの効率的誘導
口頭発表（一般）
- 日本農芸化学会 2007年度大会, 2007年, 日本語, 国内会議コリネ型細菌を用いた混合糖類からのバイオエタノール生産プロセスの構築
口頭発表（一般）
- 日本農芸化学会 2007年度大会, 2007年, 日本語, 国内会議コリネ型細菌におけるarabinose代謝経路の構築
口頭発表（一般）
- The 29th Symposium on Biotechnology for Fuels and Chemicals, 2007年, 英語, CO, USA, 国際会議Improved ethanologenic Corynebacterium glutamicum strains for fuel ethanol production from lignocellulosic biomass
ポスター発表
- The 4th Annual World Congress on Industrial Biotechnology and Bioprocessing, 2007年, 英語, FL, USA, 国際会議Ethanol production from mixed sugars by genetically engineered Coyrnebacterium glutamicum
ポスター発表
- American Chemical Society 234th National Meeting & Exposition, 2007年, 英語, MA, USA, 国際会議Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum
ポスター発表
- 日本生物工学会 2006年度大会, 2006年, 日本語, 国内会議嫌気代謝経路改変大腸菌によるグルコースからの高収率水素生成
口頭発表（一般）
- 日本生物工学会 2006年度大会, 2006年, 日本語, 国内会議コリネ型細菌を用いたバイオエタノール生産プロセスの構築に関する基礎的検討
口頭発表（一般）
- 日本農芸化学会 2006年度大会, 2006年, 日本語, 国内会議コリネ型細菌におけるxylose代謝経路の構築
口頭発表（一般）
- 日本農芸化学会 2006年度大会, 2006年, 日本語, 国内会議FHL高発現大腸菌による蟻酸からの高速水素生成法
口頭発表（一般）
- AIChE, The 2006 Annual Meeting, 2006年, 英語, CA, USA, 国際会議Conversion of mixed sugars into ethanol by recombinant Corynebacterium Glutamicum
口頭発表（一般）
- 日本農芸化学会 2005年度大会, 2005年, 日本語, 国内会議酸素抑制条件下におけるコリネ型細菌の糖代謝経路の解析
ポスター発表
- International Workshop on Biorefinery, 2005年, 英語, Kyoto, Japan, 国際会議High-efficiency hydrogen production from formate
ポスター発表
- 27th Symposium in Biotechnology for Fuels and Chemicals, 2005年, 英語, CO, USA, 国際会議Development of a new bioprocess for organic acid production using Corynebacterium glutamicum
ポスター発表
- The 2nd Annual World Congress on Industrial Biotechnology and Bioprocessing, 2005年, 英語, FL, USA, 国際会議A novel bioprocess for the production of fuel ethanol and chemicals from lignocellulosic sugars
ポスター発表
- International Workshop on Biorefinery, 2005年, 英語, Kyoto, Japan, 国際会議A novel bioprocess for the production of fuel ethanol and chemicals from lignocellulosic sugars
ポスター発表
- 日本農芸化学会 2004年度大会, 2004年, 日本語, 国内会議組換えコリネ型細菌を利用した木材からの高効率エタノール生産：発酵阻害物質の影響について
口頭発表（一般）
- The World Congress on Industrial Biotechnology and Biprocessing, 2004年, 英語, FL, USA, 国際会議Production of green products from biomass by novel bioprocess
ポスター発表
- 日本農芸化学会 2003年度大会, 2003年, 日本語, 国内会議組み換えコリネ型細菌を利用したエタノール生産
口頭発表（一般）
- 日本農芸化学会 2003年度大会, 2003年, 日本語, 国内会議バイオ水素生産：高密菌体反応および遺伝子改良による高生産性への試み
口頭発表（一般）
- 日本農芸化学会 2003年度大会, 2003年コリネ型細菌を用いた新規バイオプロセスの開発：代謝制御機構の解明
- 25th Symposium in Biotechnology for Fuels and Chemicals, 2003年, 英語, CO, USA, 国際会議Efficient ethanol production by a novel bioprocess
ポスター発表
- 日本生物工学会 2001年度大会, 2001年藻体バイオマス由来成分による光合成細菌の水素生産促進効果
- 日本農芸化学会 2000年度大会, 2000年, 日本語, 国内会議乳酸菌と光合成細菌から成る混合培養を利用した藻体バイオマスからの水素生産
口頭発表（一般）
- 日本生物工学会 2000年度大会, 2000年, 日本語, 国内会議藻体バイオマスを基質とする光水素生産 ―乳酸菌と光合成細菌の混合培養系の検討―
口頭発表（一般）
- 日本生物工学会 2001年度大会, 2000年, 日本語, 国内会議光合成細菌を用いた水素生産系における廃バイオマスの利用
口頭発表（一般）
- 日本生物工学会 1998年度大会, 1998年, 日本語, 国内会議デンプンを基質とする光水素生産における微生物共生系の機能解析
口頭発表（一般）
- 日本農芸化学会2022年度京都大会, 2022年03月17日, 英語Itaconic acid production from hydrolysates of lignocellulosic biomass by recombinant Corynebacterium glutamicum
口頭発表（一般）
- 日本農芸化学会2022年度京都大会, 2022年03月17日, 日本語酸素制限条件におけるコリネ型細菌のアミノ酸代謝についてのメタボローム解析
口頭発表（一般）
- 第16回日本ゲノム微生物学会年会, 2022年03月03日, 日本語FBAシミュレーションを用いた代謝デザインによるイタコン酸生産コリネ菌の開発
ポスター発表
所属学協会
共同研究・競争的資金等の研究課題
- 新エネルギー・産業技術総合開発機構 (NEDO), ムーンショット型研究開発事業／2050年までに、地球環境再生に向けた持続可能な資源循環を実現, 2020年07月 - 2030年02月, 研究分担者光スイッチ型海洋分解性の可食プラスチックの開発研究
- 独立行政法人環境再生保全機構, 環境研究総合推進費, 2020年04月 - 2023年03月, 研究分担者バイオマス廃棄物由来イタコン酸からの海洋分解性バイオナイロンの開発
- 日本学術振興会, 学術研究助成基金助成金／基盤研究(B)(特設分野）, 2019年07月 - 2022年03月, 研究代表者植物の資源ポテンシャルを活用した微生物発酵による新しいバイオリファイナリー
競争的資金
- 科学技術振興機構, 未来社会創造事業（探索加速型：地球規模課題である低炭素社会の実現）, 「ゲームチェンジングテクノロジー」による低炭素社会の実現, 2018年04月 - 2021年05月, 研究分担者雑種強勢の原理解明によるバイオマス技術革新
競争的資金
- 日本学術振興会, 学術研究助成基金助成金／基盤研究(C), 2016年04月 - 2021年03月, 研究代表者
競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業 先端的低炭素化技術開発（ALCA）, 2015年10月 - 2020年03月微生物バイオマスを用いたスーパーエンジニアリングプラスチックの創出
競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業 先端的低炭素化技術開発（ALCA）, 2015年10月 - 2019年03月バイオマス由来のセルローナノファを用いた“しなやかな” 高分子複合材料の創出
競争的資金
- 石油天然ガス・金属鉱物資源機構, JOGMEC-東京大学共同研究, 2008年08月 - 2013年01月石油・天然ガス開発・探鉱のフロンティア分野における先導的研究
競争的資金
- 日本学術振興会, 学術研究助成基金助成金／基盤研究(A), 2008年04月 - 2013年03月二酸化炭素処理とエネルギー資源再生を両立させる持続型炭素循環システムの最適化技術
競争的資金
- 科学技術振興機構 (JST), 未来社会創造事業「地球規模課題である低炭素社会の実現」領域 本格研究, 名古屋大学, 2021年06月 - 2026年03月, 研究分担者
少ない資源投入量でも、生育旺盛でロバストな高バイオマス・高糖性ソルガム新品種(純系やF1)の育種デザイン、創出、及び社会実装を進める。具体的には、(1) 社会実装に重要な新規遺伝子の同定し、 (2) その新規遺伝子、及び F1の雑種強勢に重要な5遺伝子を活用した集積育種を進め、(3) ソルガムの社会実装を想定した栽培試験を行う。また、(4)バイオリファイナリーにおける発酵生産技術開発として、成分分析及びプラットフォーム微生物への影響評価をカタログ化も行う。
産業財産権
L−アラビノース利用機能を有するコリネ型細菌形質転換体
特願2007-222439, 2007年08月29日, 公益財団法人地球環境産業技術研究機構, 特開2009-050236, 2009年03月12日, 特許第5074131号, 2012年08月31日特許権
エタノールの新規製造方法
特願2003-146716, 2003年05月23日, 財団法人地球環境産業技術研究機構, 特開2004-344107, 2004年12月09日, 特許第4294373号, 2009年04月17日特許権
バイオマスから糖類を製造する方法
特願2015-137531, 2015年07月09日, 国立大学法人神戸大学, 特開2017-018023, 2017年01月26日, 特許第6665998号特許権