研究者紹介システム

石井 尊生
イシイ タカシゲ
大学院農学研究科 資源生命科学専攻
教授
農学関係
Last Updated :2021/05/12

研究者情報

所属

  • 【主配置】

    大学院農学研究科 資源生命科学専攻
  • 【配置】

    農学部 資源生命科学科, 大学院農学研究科 附属食資源教育研究センター

学位

  • 農学博士, 京都大学

授業科目

ジャンル

  • 環境・交通 / 農業

コメントテーマ

  • 植物育種学
  • 植物遺伝
  • 熱帯アジアの野生イネ集団
  • 有用遺伝子
  • イネの栽培過程の解明
  • 生物多様性

研究活動

研究キーワード

  • 植物遺伝
  • 植物育種

研究分野

  • 環境・農学 / 遺伝育種科学
  • ライフサイエンス / 多様性生物学、分類学
  • ライフサイエンス / 遺伝学

委員歴

  • 日本育種学会, 幹事

受賞

  • 2018年09月 日本育種学会, 第134回講演会日本育種学会優秀発表賞 (2), Exomeと果実・葉形状にみる日本のウメの多様性

    沼口孝司, 赤木剛士, 北村祐人, 大江孝明, 石川亮, 石井尊生

    国内学会・会議・シンポジウム等の賞

  • 2018年09月 日本育種学会, 第134回講演会日本育種学会優秀発表賞, ジャポニカ型栽培イネ(日本晴)の非脱粒性に関与する新規遺伝子座の離層形成への効果

    杉山 昇平, 辻村 雄紀, 大塚 宏太郎, Htun, Than Myint, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2016年09月 日本育種学会, 第130回講演会日本育種学会優秀発表賞, Oryza rufipogonとインディカ型栽培イネ(カサラス)の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2014年09月 日本育種学会, 第126回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用と離層形態

    井上 千鶴, Than Myint Htun, 井上 加奈子, 池田 健一, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2014年03月 日本育種学会, 第125回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用

    井上 千鶴, Than Myint Htun, 石井 尊生, 石川 亮

    国内学会・会議・シンポジウム等の賞

  • 2013年10月 神戸大学, 学長表彰, 顕著な研究功績

    石井 尊生

    その他の賞

  • 2013年09月 六篠会, 六篠国際賞, 顕著な研究功績

    石井 尊生

    その他の賞

論文

  • Yaddehige P.J. Amarasinghe, Mitsuharu Otsuka, Sathya Lim, Ryo Ishikawa, Takashige Ishii

    AbstractCultivated rice (Oryza sativa L.) was domesticated from the Asian wild species O. rufipogon. Compared with cultivated rice, wild rice has spikelets/seeds with long barbed awns. In order to evaluate the role of awns in wild rice, four seed groups with different awn lengths (full, half, quarter and no awns) were prepared, and the following seed dispersal movements were investigated under simulated natural conditions as observed in the tropical Asian habitat: (1) seed detachment from the parent plant; (2) falling angle of mature seed; (3) ability to slip into small spaces; (4) horizontal movement on the ground and (5) horizontal movement in water. As a result, awns were found to enhance the detachment of matured seeds from the panicles in the initial seed dispersal step. They regulated vertical orientation in the air, and the vertical form may enable seeds to squeeze to the ground. The awned seeds also showed advantages in horizontal movements on the ground and in the water. In most of the experiments, seeds with full awns showed the best performance for seed dispersal, suggesting that wild rice keeps long awns to survive under natural conditions. Since seed awning is dominantly controlled by wild functional alleles at several loci, wild rice may be able to prevent a drastic reduction of awn length.

    Cambridge University Press (CUP), 2020年12月, Seed Science Research, 30 (4), 319 - 324

    [査読有り]

    研究論文(学術雑誌)

  • Koji Numaguchi, Takashi Akagi, Yuto Kitamura, Ryo Ishikawa, Takashige Ishii

    Domestication and population differentiation in crops involve considerable phenotypic changes. The logs of these evolutionary paths, including natural/artificial selection, can be found in the genomes of the current populations. However, these profiles have been little studied in tree crops, which have specific characters, such as long generation time and clonal propagation, maintaining high levels of heterozygosity. We conducted exon-targeted resequencing of 129 genomes in the genus Prunus, mainly Japanese apricot (Prunus mume), and apricot (Prunus armeniaca), plum (Prunus salicina), and peach (Prunus persica). Based on their genome-wide single-nucleotide polymorphisms merged with published resequencing data of 79 Chinese P. mume cultivars, we inferred complete and ongoing population differentiation in P. mume. Sliding window characterization of the indexes for genetic differentiation identified interspecific fragment introgressions between P. mume and related species (plum and apricot). These regions often exhibited strong selective sweeps formed in the paths of establishment or formation of substructures of P. mume, suggesting that P. mume has frequently imported advantageous genes from other species in the subgenus Prunus as adaptive evolution. These findings shed light on the complicated nature of adaptive evolution in a tree crop that has undergone interspecific exchange of genome fragments with natural/artificial selections.

    2020年10月13日, The Plant Journal : for cell and molecular biology, 104, 1551 - 1567, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Yaddehige Priya Jayantha Amarasinghe, Rie Kuwata, Akinori Nishimura, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    Background Awns are bristle-like organs at the tips of the glumes. Wild rice has maintained long awns for successful seed propagation through seed dispersal. Seed awning is an interesting trait in rice domestication. Long awns might have been beneficial for seed gatherers in the initial phase of domestication; however, awnless phenotypes were preferably selected in a later phase with non-seed-shattering plants. Investigation of domestication loci associated with awnlessness in cultivated rice will be useful in clarifying the process and history of rice domestication. Results Quantitative trait locus (QTL) analysis for seed awning was carried out using a BC3F2 population between Oryza sativa IR36 (a cultivated donor parent with awnless phenotype) and O. rufipogon W630 (a wild recurrent parent with awns). As a result, two QTLs on chromosome 4 (corresponding to An-1 and LABA1) and one on chromosome 2 (designated as qAWNL2) were detected. Gene interaction among three seed-awning QTLs were further examined with the plants having eight different combinations of homozygous genotypes. Their awn length variation indicated that the IR36 alleles at these loci had the additive awnlessness effects in the genetic background of wild rice. The shortest awn length was observed for the plants having IR36 homozygous alleles at all loci, giving about 75% reduction in awn length. By the fine mapping, the candidate region of the novel qAWNL2 locus was delimited in a 157.4-kb region containing 22 predicted genes in Nipponbare genome. Conclusions QTL analysis revealed that three loci, An-1, LABA1 and qAWNL2, were mainly responsible for the awnlessness of O. sativa IR36. In the wild genetic background, loss-of-function alleles at three awning loci showed additive effects on length reduction. In rice domestication, awnless forms may be gradually generated through the accumulation of mutations at awning loci.

    SPRINGER, 2020年04月, RICE, 13 (1), 26 - 26, 英語, 国際誌

    [査読有り]

    研究論文(学術雑誌)

  • Rieko Shishido, Masahiro Akimoto, Tin Htut, Tadashi Sato, Takashige Ishii

    To evaluate genetic diversity and genetic structure of wild rice (Oryza rufipogon) populations in Myanmar, seven research sites were selected based on various ecological conditions. A large number of samples under natural growth conditions were collected and studied using six simple sequence repeats (SSRs) and two chloroplast DNA markers. A total of 77 alleles were detected from 1559 samples over six SSR loci. The mean number of alleles per population ranged from 3.167 to 8.667, and the mean expected heterozygosity ranged from 0.140 to 0.701. Wild rice populations survived under various environmental conditions and retained different levels of genetic diversity. The large number of samples was effective to confirm the spatial genetic structure of wild rice populations in a relatively small area. Regarding chloroplast DNA polymorphisms, four populations possessed only one pattern, while the other three showed two or five combinations of haplotypes, even within the same population. Additionally, the existence of a new genotype was revealed. Considerable variations in chloroplast DNA exist in the wild rice populations of Myanmar. A high proportion of genetic variation was detected within, rather than among, populations. To ensure maintenance of allelic diversity, it is advisable to preserve many individuals from a large population.

    2019年09月, Breeding Science, 69 (3), 471 - 477, 英語, 国内誌

    [査読有り]

    研究論文(学術雑誌)

  • Spikelet opening under controlled transpiration conditions in rice

    Funa Ogawa, Ryo Ishikawa, Takashige Ishii

    2019年, Journal of Crop Research, 64, 31 - 35, 英語

    [査読有り]

    研究論文(学術雑誌)

  • QTL analysis for awnlessness using a backcross population between Oryza rufipogon and O. sativa IR36

    Yaddehige P, J. Amarasinghe, Rie Kuwata, Ryo Ishikawa, Takashige Ishii

    2019年, Journal of Crop Research, 64, 43 - 46, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Koji Numaguchi, Shogo Ishio, Yuto Kitamura, Kentaro Nakamura, Ryo Ishikawa, Takashige Ishii

    Japanese Society for Horticultural Science, 2019年, The Horticulture Journal, 88 (2), 222 - 231, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Detection of a novel locus involved in non-seed-shattering behaviour of Japonica rice cultivar, Oryza sativa 'Nipponbare'

    Yuki Tsujimura, Shohei Sugiyama, Kotaro Otsuka, Than Myint Htun, Koji Numaguchi, Cristina Castillo, Takashi Akagi, Takashige Ishii, Ryo Ishikawa

    2019年, Theor. Appl. Genet., 132, 2615 - 2623, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takashige Ishii, Ryo Ishikawa

    Cultivated rice (Oryza sativa L.) was domesticated from the Asian wild species, Oryza rufipogon Griff. Among morphological differences between them, one of the striking traits specific to cultivated rice is loss of seed shattering. In the early stage of rice domestication, the related traits of this character have been desirable for the ancient seed gatherers because it enhances the efficiency of seed collection. In this chapter, we propose that three morphological traits, closed panicle shape, non-seed shattering, and seed awning, played important roles in controlling the degree of seed dispersal. First, we reviewed domestication loci controlling the three traits. We then evaluated allele effects at these loci using reciprocal backcross populations between O. sativa Nipponbare and our standard wild accession of O. rufipogon W630. In the genetic background of cultivated rice, all the wild functional alleles were responsible for these domestication traits. On the other hand, cultivated non-functional alleles were not always associated with the drastic morphological changes in the genetic background of wild rice. Since ancient humans have selected cultivated-type mutants in natural wild populations, possible domestication process for the emergence of cultivated rice is discussed based on the effects of cultivated non-functional alleles.

    Springer Singapore, 2018年03月15日, Rice Genomics, Genetics and Breeding, 207 - 221, 英語

    [査読有り]

    論文集(書籍)内論文

  • Ryo Ishikawa, Masahide Iwata, Kenta Taniko, Gotaro Monden, Naoya Miyazaki, Chhourn Orn, Yuki Tsujimura, Shusaku Yoshida, Jian Feng Ma, Takashige Ishii

    Zinc (Zn) is one of the essential mineral elements for both plants and humans. Zn deficiency in human is one of the major causes of hidden hunger, a serious health problem observed in many developing countries. Therefore, increasing Zn concentration in edible part is an important issue for improving human Zn nutrition. Here, we found that an Australian wild rice O. meridionalis showed higher grain Zn concentrations compared with cultivated and other wild rice species. The quantitative trait loci (QTL) analysis was then performed to identify the genomic regions controlling grain Zn levels using backcross recombinant inbred lines derived from O. sativa 'Nipponbare' and O. meridionalis W1627. Four QTLs responsible for high grain Zn were detected on chromosomes 2, 9, and 10. The QTL on the chromosome 9 (named qGZn9), which showed the largest effect on grain Zn concentration was confirmed with the introgression line, which had a W1627 chromosomal segment covering the qGZn9 region in the genetic background of O. sativa 'Nipponbare'. Fine mapping of this QTL resulted in identification of two tightly linked loci, qGZn9a and qGZn9b. The candidate regions of qGZn9a and qGZn9b were estimated to be 190 and 950 kb, respectively. Furthermore, we also found that plants having a wild chromosomal segment covering qGZn9a, but not qGZn9b, is associated with fertility reduction. qGZn9b, therefore, provides a valuable allele for breeding rice with high Zn in the grains.

    PUBLIC LIBRARY SCIENCE, 2017年10月, PLOS ONE, 12 (10), e0187224, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ryo Ishikawa, Akinori Nishimura, Than Myint Htun, Ryo Nishioka, Yumi Oka, Yuki Tsujimura, Chizuru Inoue, Takashige Ishii

    Rice (Oryza sativa L.) is widely cultivated around the world and is known to be domesticated from its wild form, O. rufipogon. A loss of seed shattering is one of the most obvious phenotypic changes selected for during rice domestication. Previously, three seed-shattering loci, qSH1, sh4, and qSH3 were reported to be involved in non-shattering of seeds of Japonica-type cultivated rice, O. sativa cv. Nipponbare. In this study, we focused on non-shattering characteristics of O. sativa Indica cv. IR36 having functional allele at qSH1. We produced backcross recombinant inbred lines having chromosomal segments from IR36 in the genetic background of wild rice, O. rufipogon W630. Histological and quantitative trait loci analyses of abscission layer formation were conducted. In the analysis of quantitative trait loci, a strong peak was observed close to sh4. We, nevertheless, found that some lines showed complete abscission layer formation despite carrying the IR36 allele at sh4, implying that non-shattering of seeds of IR36 could be regulated by the combination of mutations at sh4 and other seed-shattering loci. We also genotyped qSH3, a recently identified seed-shattering locus. Lines that have the IR36 alleles at sh4 and qSH3 showed inhibition of abscission layer formation but the degree of seed shattering was different from that of IR36. On the basis of these results, we estimated that non-shattering of seeds in early rice domestication involved mutations in at least three loci, and these genetic materials produced in this study may help to identify novel seed-shattering loci.

    SPRINGER, 2017年04月, GENETICA, 145 (2), 201 - 207, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    Seed awning is one of the important traits for successful propagation in wild rice. During the domestication of rice by ancient humans, plants with awnless seeds may have been selected because long awns hindered collection and handling activities. To investigate domestication of awnless rice, QTL analysis for seed awning was first carried out using backcross recombinant inbred lines between Oryza sativa Nipponbare (recurrent parent) and O. rufipogon W630 (donor parent). Two strong QTLs were detected in the same regions as known major seed-awning loci, An-1 and RAE2. Subsequent causal mutation surveying and fine mapping confirmed that O. rufipogon W630 has functional alleles at both loci. The gene effects and interactions at these loci were examined using two backcross populations with reciprocal genetic backgrounds of O. sativa Nipponbare and O. rufipogon W630. As awn length in wild rice varied among seeds even in the same plant, awn length was measured based on spikelet position. In the genetic background of cultivated rice, the wild alleles at An-1 and RAE2 had awning effects, and plants having both wild homozygous alleles produced awns whose length was about 70% of those of the wild parent. On the other hand, in the genetic background of wild rice, the substitution of cultivated alleles at An-1 and RAE2 contributed little to awn length reduction. These results indicate that the domestication process of awnless seeds was complicated because many genes are involved in awn formation in wild rice.

    GENETICS SOC JAPAN, 2017年02月, GENES & GENETIC SYSTEMS, 92 (1), 21 - 26, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Investigation of genetic loci controlling non-shattering behaviour in an Indica rice cultivar ‘IR36’. Journal of Crop Research 62: 19-23.

    Yuki Tsujimura, Chizuru Inoue, Than Mynt Htun, Yumi Oka, Takashige Ishii, Ryo Ishikawa

    2017年, Journal of Crop Research, 62, 19 - 23, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Identification of quantitative trait loci controlling floral morphology of rice using a backcross population between common cultivated rice, Oryza sativa and Asian wild rice, O. rufipogon

    Ryo Ishikawa, Takafumi Watabe, Ryo Nishioka, Pham Tien Thanh, Takashige Ishii

    2017年, American Journal of Plant Science, 8, 734 - 744, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Genetic survey of genomic regions responsible for panicle spreading in wild rice, Oryza rufipogon

    Ryo Nishioka, Hiromi Mikazuki, Than Myint Htun, Ryo Ishikawa, Takashige Ishii

    2016年, Journal of Crop Research, 61, 23 - 26, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Evaluation of shooting and rooting ability from nodes and ratooning ability using backcross recombinant inbred lines between Oryza rufipogon and O. sativa

    Mai Ikemoto, Tetsuya Ohyagi, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii

    2016年, Journal of Crop Research, 61, 13 - 17, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chhourn Orn, Rieko Shishido, Masahiro Akimoto, Ryo Ishikawa, Than Myint Htun, Ken-Ichi Nonomura, Yohei Koide, Men Sarom, Seng Vang, Sakhan Sophany, Ouk Makara, Takashige Ishii

    A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources.

    JAPANESE SOC BREEDING, 2015年12月, BREEDING SCIENCE, 65 (5), 430 - 437, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hao Yin, Masahiro Akimoto, Reunreudee Kaewcheenchai, Masahiro Sotowa, Takashige Ishii, Ryuji Ishikawa

    AA genome species in the genus Oryza are valuable resources for improvement of cultivated rice. Oryza rufipogon and O. barthii were progenitors of two domesticated rice species, O. sativa and O. glaberrima, respectively. We used chloroplast single-nucleotide repeats (RCt1-10) to evaluate genetic diversity among AA genome species. Higher diversity was detected in the American species O. glumaepatula and the Asian species O. rufipogon. Other chloroplast sequences indicated that O. glumaepatula shares high similarity with O. longistaminata. Insertions of retrotransposable elements, however, showed a close relation between O. barthii and O. glumaepatula. To clarify phylogenetic relationships among AA genomes, whole-genome sequences obtained from different species were used to develop chloroplast INDEL markers. The INDEL patterns clearly showed multiple maternal origins of O. glumaepatula. The complicated origins have resulted in high genetic diversity in this species. In contrast, the Australian endemic species O. meridionalis tended to show narrower diversity than the other species. High variation in O. rufipogon, reconfirmed using the chloroplast INDELs, covered the variation in O. meridionalis and part of the variation in O. glumaepatula. Maternal lineages including O. barthii, O. longistaminata and the remainder of O. glumaepatula were phylogenetically close to each other and carried low genetic diversity. They were separated from independent lineages, suggesting that they had diverged from a single ancestral maternal lineage, but diverged later to keep gene flow within respective species, as SSR compositions suggested. Genetic relationships among AA genome species indicate how these species have evolved and become distributed across four continents.

    GENETICS SOC JAPAN, 2015年10月, GENES & GENETIC SYSTEMS, 90 (5), 269 - 281, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Inhibition of abscission layer formation by an interaction of two seed-shattering loci, sh4 and qSH3, in rice

    Chizuru Inoue, Than Myint Htun, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa

    Loss of seed shattering was one of the key phenotypic changes selected for in the domestication of many crop species. Asian cultivated rice, Oryza sativa L., was domesticated from its wild ancestor, 0. rufipogon, and three seed-shattering loci, qSH1, sh4 and qSH3, have been reported to be involved in the loss of seed shattering in cultivated rice. Here, we analysed the seed-shattering behaviour of wild rice using introgression lines carrying the cultivated alleles from 0. sativa Nipponbare in the genetic background of wild rice, 0. rufipogon W630. We first carried out fine mapping of the qSH3 region and found that the qSH3 locus is localized in an 850-kb region on chromosome 3. We then analysed the effects of the Nipponbare alleles at sh4 and qSH3 on seed-shattering behaviour in wild rice, as a mutation at qSH1 was not commonly found in rice cultivars. Seed-shattering behaviour did not change in the two types of introgression line independently carrying the Nipponbare-homozygous alleles at sh4 or qSH3 in the genetic background of wild rice. However, the introgression lines having the Nipponbare-homozygous alleles at both sh4 and qSH3 showed a reduction in the degree of seed shattering. Histological and scanning electron microscopy analyses revealed that abscission layer formation was inhibited around the vascular bundles in these lines. Since the qSH3 region, as well as sh4, has been shown to be under artificial selection, the interaction of mutations at these two loci may have played a role in the initial loss of seed shattering during rice domestication.

    GENETICS SOC JAPAN, 2015年02月, GENES & GENETIC SYSTEMS, 90 (1), 1 - 9, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Identification of QTL for Striga hermonthica resistance using backcross population derived from a cross between Oryza sativa (cv. Nipponbare) and O. rufipogon

    Atera AE, Onyango JC, Thanh PT, Ishii T, Itoh K

    2015年, J Agr Sci, 7, 99 - 105, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Aloka Lanka Ranawake, Oliver Escano Manangkil, Shinya Yoshida, Takashi Ishii, Naoki Mori, Chiharu Nakamura

    Cold tolerance is an important breeding target in rice production. We studied quantitative trait loci (QTLs) controlling cold tolerance at germination (CTG) and early seedling (CTS) stages, using recombinant inbred lines derived from a japonica x indica cross. CTG was evaluated based on the percentage rate of germination at 15 degrees C for 12 days after pre-incubation of imbibed seeds at 20 degrees C for 2 days. For CTS, seven-day-old seedlings grown at 25 degrees C were subjected to two consecutive periods of three-day cold stress at 4 degrees C with an intervening eight-day recovery at 25 degrees C. CTS evaluation was according to an arbitrary five-point rating system at the fifth day of recovery after each stress period. No correlations were found between CTG and CTS, while a weak correlation was detected between CTS after the first and second stress. By the composite interval mapping, five QTLs for CTG explaining 5.7%-9.3% of the total phenotypic variance (PVE) and nine for CTS with PVE of 5.8%-35.6% were detected. Only one of these QTLs was common, indicating growth-stage specificity of cold tolerance. Four of the five QTLs after the second cold stress were different from the ones after the first cold stress. Analysis of variance test showed significant interactions between alleles at the QTL sites and the two stress conditions with respect to the mean CTS scores. A possible involvement of cold acclimation and usefulness of japonica germplasms in breeding for cold tolerance in indica rice was discussed.

    TAYLOR & FRANCIS LTD, 2014年11月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 28 (6), 989 - 998, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, Ryo Ishikawa

    Asian cultivated rice Oryza sativa L. was domesticated from its wild ancestor, O. rufipogon. During domestication, the cultivated rice lost its seed-shattering behaviour. Previous studies have shown that two major quantitative trait loci (QTLs; qSH1 and sh4) are responsible for the seed-shattering degree. Here, we produced introgression lines carrying non-functional alleles from O. sativa 'Nipponbare' at the two major QTLs in the genetic background of wild rice O. rufipogon W630, and examined the effects of the two QTLs on seed shattering and abscission layer formation. The introgression lines, with Nipponbare alleles at either or both loci, showed complete or partial abscission layer formation, respectively, indicating that other unknown loci might be involved in enhancing seed shattering in wild rice. We detected a single QTL named qSH3 regulating seed-shattering degree using an F-2 population between Nipponbare and the introgression line carrying Nipponbare alleles at the two QTLs. Although we generated an introgression line for qSH3 alone, no effects on seed shattering were observed. However, a significant effect on seed-shattering degree was observed for the introgression line carrying Nipponbare alleles at qSH3 and the two QTLs, suggesting an important role of qSH3 on seed shattering in coordination with the two QTLs.

    JAPANESE SOC BREEDING, 2014年09月, BREEDING SCIENCE, 64 (3), 199 - 205, 英語

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    研究論文(学術雑誌)

  • Evaluation of genetic variation among rice local and modern varieties in Cambodia

    Orn Chhourn, Ryo Ishikawa, Sakhan Sophany, Ouk Makara, Takashige Ishii

    2014年, Journal of Crop research, 59, 37 - 41, 英語

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    研究論文(学術雑誌)

  • Jeffrey A. Fawcett, Tomoyuki Kado, Eriko Sasaki, Shohei Takuno, Kentaro Yoshida, Ryuichi P. Sugino, Shunichi Kosugi, Satoshi Natsume, Chikako Mitsuoka, Aiko Uemura, Hiroki Takagi, Akira Abe, Takashige Ishii, Ryohei Terauchi, Hideki Innan

    Genes involved in the transition from wild to cultivated crop species should be of great agronomic importance. Population genomic approaches utilizing genome resequencing data have been recently applied for this purpose, although it only reports a large list of candidate genes with no biological information. Here, by resequencing more than 30 genomes altogether of wild rice Oryza rufipogon and cultivated rice O. sativa, we identified a number of regions with clear footprints of selection during the domestication process. We then focused on identifying candidate domestication genes in these regions by utilizing the wealth of QTL information in rice. We were able to identify a number of interesting candidates such as transcription factors that should control key domestication traits such as shattering, awn length, and seed dormancy. Other candidates include those that might have been related to the improvement of grain quality and those that might have been involved in the local adaptation to dry conditions and colder environments. Our study shows that population genomic approaches and QTL mapping information can be used together to identify genes that might be of agronomic importance.

    PUBLIC LIBRARY SCIENCE, 2013年12月, PLOS ONE, 8 (12), e83720 (12 pages), 英語

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    研究論文(学術雑誌)

  • CONSTRUCTION OF MICROSATELLITE LINKAGE MAP AND DETECTION OF SEGREGATION DISTORTION IN INDICA RICE (ORYZA SATIVA L.)

    Muhammad Arshad Javed, Fahrul Zaman Huyop, Takashige Ishii, Azman Abd Samad, Tariq Mahmood, Muhammad Saleem Haider, Muhammad Saleem

    High density molecular linkage map is a prerequisite to identify the quantitative traits loci. Construction of linkage maps, using mapping populations derived from two major subspecies: indica and japonica in rice, have been reported. However, limited work was conducted in indica subspecies. Present studies focused to construct a molecular map based on indica x indica (Shaheen Basmati x Pokkali) derived F-2 mapping population. The detected microsatellite polymorphism was only 50.64% between parental cultivars. A total of 107 microsatellite markers were employed to amplify 108 loci, distributed throughout the genome. The marker data of 190 individuals was recorded for map construction. Most of the markers were found co-dominant, where as eight markers exhibited dominance in favor of Shaheen Basmati alleles and four in favor of Pokkali alleles. Detected segregation distortion was 7.47%, which was significantly low than previous studies. MapMaker was used to construct the linkage groups. The map spanned 1753.9 cM (Kosambi function) with microsatellite markers on 12 rice chromosomes and an average distance of 16.2 cM between markers. This map contained a greater and least percentage of markers on linkage group 3 and 12, respectively. Maximum common interval size (MCIS) analysis revealed that present map (indica x indica) covered the rice genome 98% of previous investigation (japonica x indica). Because of diverse background of parental cultivars, low segregation distortion and high genome coverage, revealed that molecular linkage map would be used as marker framework to investigate the genetics of important agronomic traits in indica rice.

    PAKISTAN BOTANICAL SOC, 2013年12月, PAKISTAN JOURNAL OF BOTANY, 45 (6), 2085 - 2092, 英語

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    研究論文(学術雑誌)

  • Takashige Ishii, Koji Numaguchi, Kotaro Miura, Kentaro Yoshida, Pham Thien Thanh, Than Myint Htun, Masanori Yamasaki, Norio Komeda, Takashi Matsumoto, Ryohei Terauchi, Ryo Ishikawa, Motoyuki Ashikari

    Reduction in seed shattering was an important phenotypic change during cereal domestication(1,2). Here we show that a simple morphological change in rice panicle shape, controlled by the SPR3 locus, has a large impact on seed-shedding and pollinating behaviors. In the wild genetic background of rice, we found that plants with a cultivated-like type of closed panicle had significantly reduced seed shedding through seed retention. In addition, the long awns in closed panicles disturbed the free exposure of anthers and stigmas on the flowering spikelets, resulting in a significant reduction of the outcrossing rate. We localized the SPR3 locus to a 9.3-kb genomic region, and our complementation tests suggest that this region regulates the liguleless gene (OsLG1). Sequencing analysis identified reduced nucleotide diversity and a selective sweep at the SPR3 locus in cultivated rice. Our results suggest that a closed panicle was a selected trait during rice domestication.

    NATURE PUBLISHING GROUP, 2013年04月, NATURE GENETICS, 45 (4), 462 - 465, 英語

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    研究論文(学術雑誌)

  • Phan Thi Thanh, Cristian Ioan Vladutu, Shahryar F. Kianian, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Naoki Mori

    Emmer wheat (Triticum turgidum ssp. dicoccum) was a principal crop in the development and spread of Neolithic agriculture in the Old World It represents the primitive situation in the domestication of AABB tetraploid wheat. To understand the genetic modifications underlying the early stage of tetraploid wheat domestication, QTL analysis was carried out using 144 F-2 plants derived from a cross between the domesticated emmer wheat and the wild emmer wheat (T turgidum ssp. dicoccoides). To our knowledge, this is the first report of molecular linkage map and QTL analysis of the domestication-related characters in emmer wheat. The linkage map with a total length of 2849.8 cM was constructed using 227 microsatellite (SSR) markers. Chromosomal location and effect of QTLs were estimated for ten domestication-related traits including whole plant and spike characteristics. Seventeen QTLs were detected on chromosomes 1B, 2A, 2B, 3A, 3B, 4A, 5B, and 7B. Two regions on chromosomes 2A and 3B have a large effect on rachis fragility. The estimated locations of these QTLs corresponded to those of the Br genes identified in the previous studies on a more adapted durum wheat (T turgidum ssp. turgidum cony. durum). Our results indicate that selection and conversion of at least two Br loci (on chromosomes 2A and 3B) occurred during the domestication of emmer wheat prior to appearance of free-threshing wheat (e.g. durum). The map positions of nine QTLs for the traits related to seed production overlapped in two regions on chromosomes 2A and 5B. The result suggests that these chromosomal regions played an important role in increasing seed production during the domestication of emmer wheat. Biotechnol. & Biotechnol. Eq. 2013, 27(2), 3627-3637

    DIAGNOSIS PRESS LTD, 2013年04月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 27 (2), 3627 - 3637, 英語

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    研究論文(学術雑誌)

  • Striga infestation in Kenya: Status, distribution and management options

    Atera AE, Ishii T, Onyango JC, Itoh K, Azuma T

    2013年, Sust Agr Res, 2, 99 - 108, 英語

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    研究論文(学術雑誌)

  • Phuong Dang Thai Phan, Hiroki Kageyama, Ryo Ishikawa, Takashige Ishii

    The Asian wild rice, Oryza rufipogon, has partial outcrossing behavior and shows high levels of genetic variation. To estimate an accurate outcrossing rate of annual form of O. rufipogon, two backcross lines (Lines R1 and R2) between Oryza saliva Nipponbare and O. rufipogon W630 were examined under field conditions. A chromosome survey confirmed that these lines had wild chromosomal segments at more than 92% of marker loci. As for the traits of glume, stamen and pistil, Line R1 showed similar floral morphology as that of O. rufipogon W630, whereas Line R2 had larger glumes. In 2005, 2006 and 2008, a total of 22 backcross plants were planted in the middle of wild rice plots. The successive progenies of each plant were examined using microsatellite markers that could clearly detect self-pollination and outcrossing. The outcrossing rates of Line R1 plants ranged from 4.04% to 25.50% with an average of 10.20%. This indicates that cross-pollination of wild rice is a chance event affected by many environmental factors. The outcrossing rates of Line R2 plants also varied, however, no significant difference was observed between the averages of Lines RI and R2, suggesting that the glume sizes are not critical for outcrossing ability.

    JAPANESE SOC BREEDING, 2012年09月, BREEDING SCIENCE, 62 (3), 256 - 262, 英語

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    研究論文(学術雑誌)

  • Atera AE, Itoh K, Azuma T, Ishii T

    Witchweed, Striga hermonthica (hereafter, referred to as Striga), is a major biotic constraint to cereal production in sub-Saharan Africa. The parasitic plant is a socioeconomic problem that has forced some resource-poor farmers to abandon their farms due to high infestation. This study was designed in order to elucidate farmers' perceptions of Striga control measures and to determine their potential adoption in two villages in western Kenya. Participatory rural appraisals and individual interviews were conducted in 2009 and 2010 in a sample of 128 and 120 households in Kaura and Kogweno-Oriang villages in Homabay and Rachuonyo districts, respectively. The results revealed that crop production was the main occupation in most households. The farmers identified Striga as one of the major constraints to maize, sorghum, and finger millet production. According to the farmers, the most popular control measures were hand-pulling, crop rotation, and intercropping, even though rotational systems might need a longer timeframe to reduce the soil seed bank of Striga. Although the level of Striga infestation and damage were increasing in the farmers' fields, the adoption of the control options was limited. The reason for the low adoption level of the control methods by the farmers is because they are too risky as there is no guarantee of a direct pay-off in increased crop yield. Farmer-led evaluation and adaptation of the various Striga control technologies in real-life situations will facilitate the choice of appropriate options and facilitate their uptake.

    WILEY-BLACKWELL, 2012年03月, Weed Biol Manag, 12 (1), 53 - 62, 英語

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    研究論文(学術雑誌)

  • Kazuyuki Mori, Nobuhiro Mukojima, Takashi Nakao, Seiji Tamiya, Yu Sakamoto, Norio Sohbaru, Kazuya Hayashi, Hitomi Watanuki, Kazuhiro Nara, Kaoru Yamazaki, Takashige Ishii, Kazuyoshi Hosaka

    Saikai 35 was bred from a cross between TD0101 as the female, which was created by chromosome-doubling of a good-tasting and bacterial wilt resistant diploid variety, Inca-no-mezame, and Sakurafubuki as the male, the latter of which has H1 and Ry (chc) genes showing resistance to potato cyst nematode (PCN) and Potato virus Y (PVY), respectively. All favorable traits were combined into Saikai 35, although marketable yield in the spring cropping was 20.4-21.0% lower than those of major double-cropping varieties. Saikai 35 is particularly useful for having Solanum phureja-derived cytoplasm (S/epsilon), which resulted in high male and female fertility. In addition, sets of very tightly linked DNA markers sandwiching H1 and Ry (chc) are available. Therefore, Saikai 35 is being released as a breeding line, which can confer efficiently PCN and PVY resistance genes.

    SPRINGER, 2012年02月, AMERICAN JOURNAL OF POTATO RESEARCH, 89 (1), 63 - 72, 英語

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    研究論文(学術雑誌)

  • Response of NERICA Rice to Striga hermonthica Infections in Western Kenya

    Evans A. Atera, Kazuyuki Itoh, Tetsushi Azuma, Takashige Ishii

    Striga hermonthica (Del.) Benth (hereafter referred to as Striga), an obligate root hemiparasite, poses a serious threat to cereal production in sub-Saharan Africa. Field experiments were conducted in two years at Alupe farm, western Kenya, to investigate the effect of Striga on growth and yield parameters of New Rice for Africa (NERICA) cultivars. A randomized complete block design replicated three times and rice cultivars NERICA 1, NERICA 4, NERICA 10, NERICA 11 and Dourado precoce, a local landrace were used. Striga significantly reduced grain yield and the yield components. Reduction in grain yield and its components were more severe under moisture stress period in 2008. Grain yield loss ranged between 33-90%. NERICA 1 gave the highest yield in the two seasons both in Striga infected and control plants. This was followed by NERICA 10, which was also the most economically viable when infected with Striga. Result showed that both NERICA 1 and NERICA 10 are resistant to S. hermonthica, while NERICA 4 is highly susceptible. (C) 2012 Friends Science Publishers

    FRIENDS SCIENCE PUBL, 2012年, INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, 14 (2), 271 - 275, 英語

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    研究論文(学術雑誌)

  • Farmers’ perception and constraints to the adoption of weed control options: the case of Striga asiatica in Malawi

    Atera AE, Itoh K, Azuma T, Ishii T

    2012年, J Agr Sci, 4, 41 - 50, 英語

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    研究論文(学術雑誌)

  • Takashige Ishii, Takashi Hiraoka, Tomoyuki Kanzaki, Masahiro Akimoto, Rieko Shishido, Ryo Ishikawa

    Cultivated rice (Oryza sativa L.) is derived from Asian wild rice (Oryza rufipogon Griff). Vietnamese local varieties and wild natural populations in Vietnam and Myanmar were examined to evaluate the levels of genetic variation in cultivated and wild rice. In total, 222 Vietnamese local varieties were analyzed with ten microsatellite markers. Using marker genotype and gene diversity data, the local varieties were differentiated based on geographical distribution, cropping season, and human preference. A total of 976 wild plants were collected at six natural sites of wild populations (three each in Myanmar and Vietnam), and the degrees of variation among populations were analyzed with five microsatellite markers. Phylogenetic analyses revealed wide genetic differentiation among wild populations. The diversity values detected in a single wild population in Vietnam were higher than those in whole Vietnamese local varieties. These results indicate that wild rice has much greater genetic variation than cultivated rice.

    SPRINGER, 2011年12月, RICE, 4 (3-4), 170 - 177, 英語

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    研究論文(学術雑誌)

  • Kazuyuki Mori, Yu Sakamoto, Nobuhiro Mukojima, Seiji Tamiya, Takashi Nakao, Takashige Ishii, Kazuyoshi Hosaka

    Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry (chc) ) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples, were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially harboring all five resistance genes were selected from 96 hybrid plants approximately 5 h after DNA extraction, which is a third of the operation time compared with separate PCR reactions for each marker.

    SPRINGER, 2011年08月, EUPHYTICA, 180 (3), 347 - 355, 英語

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    研究論文(学術雑誌)

  • Pham Thien Thanh, Phuong Dang Thai Phan, Naoki Mori, Ryo Ishikawa, Takashige Ishii

    Backcross populations at advanced generations are useful in evaluating the agronomic traits from unadapted germplasms, such as wild species. In this study, we generated 159 backcross recombinant inbred lines (BRILs) at the BC2F8 generation between Oryza saliva Nipponbare (a recurrent parent) and O. rufipogon W630 (a donor parent). These BRILs were found to have nearly homozygous genome constitutions based on the marker genotypes at 180 SSR loci covering whole chromosomes. Since their selfed progenies have almost identical genotypes in each line, they were further used for QTL analysis of drought tolerance. Out of six QTLs detected, three were found to have wild alleles with favorable effects on drought tolerance. The present BRILs with known marker data can be applied to more QTL analyses of traits specific to wild species.

    JAPANESE SOC BREEDING, 2011年03月, BREEDING SCIENCE, 61 (1), 76 - 79, 英語

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    研究論文(学術雑誌)

  • Temporal changes of seed shattering degree of substitution lines having non-shattering alleles from cultivated rice (Oryza sativa) in the genetic background of wild rice (O. rufipogon)

    Than Myint Htun, Takashige Ishii, Ryo Ishikawa

    2011年, Journal of Crop research, 56, 39 - 44, 英語

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    研究論文(学術雑誌)

  • QTL analysis for submergence avoidance using backcross recombinant inbred lines between Oryza sativa and O. rufipogon.

    Phuong Dang, Thai Phan, Pham Thien Thanh, Naoki Mori, Ryo Ishikawa, Takashige Ishii

    2011年, Journal of Crop research, 56, 45 - 49, 英語

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    研究論文(学術雑誌)

  • QTL analysis for flowering time using backcross population between Oryza sativa Nipponbare and O. rufipogon

    Pham Thien Thanh, Phuong Dang Thai Phan, Ryo Ishikawa, Takashige Ishii

    In the near future, global average temperature is expected to increase due to the accumulation of greenhouse gases, and increased temperatures will cause severe sterility in many crop species. In rice, since wild species show high genetic variation, they may have the potential to improve the flowering characters of cultivars. In this study, we investigated flowering characters under natural conditions by comparing an Asian wild rice accession of Oryza rufipogon W630 (originated from Myanmar) with a Japanese rice cultivar, O. sativa Japonica cv. Nipponbare. Further, QTL analysis for days to heading (DH) and spikelet opening time (SOT: the time of day when the spikelet opens) was carried out using BC2F8 backcross population derived from the cross between them. Regarding DH, four QTLs were detected, and two of them were found to have wild alleles with strong effects leading to longer days to heading during the Japanese summer. These wild alleles may be used to produce late-heading cultivars that do not flower during the high summer temperatures anticipated in the future. As for SOT, two parameters of SOTb (beginning time when the first spikelet opens) and SOTm (median time when 50% of the spikelets open) were recorded and the time differences from Nipponbare were investigated. Two QTLs on chromosomes 5 and 10 and two QTLs on chromosomes 4 and 5 were detected for SOTb and SOTm, respectively. The wild alleles were responsible for early spikelet opening time at all loci. If the wild alleles detected in this study have the same effects in the genetic background of other cultivars, they will be very useful in producing early-flowering rice cultivars that complete fertilization in the morning before the temperature rises.

    GENETICS SOC JAPAN, 2010年08月, GENES & GENETIC SYSTEMS, 85 (4), 273 - 279, 英語

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    研究論文(学術雑誌)

  • Allelic interaction at seed-shattering loci in the genetic backgrounds of wild and cultivated rice species

    Ryo Ishikawa, Pham Thien Thanh, Naoto Nimura, Than Myint Htun, Masanori Yamasaki, Takashige Ishii

    It is known that the common cultivated rice (Oryza sativa) was domesticated from Asian wild rice, O. rufipogon. Among the morphological differences between them, loss of seed shattering is one of the striking characters specific for the cultivated forms. In order to understand the genetic control on shattering habit, QTL analysis was carried out using BC2F1 backcross population between O. sativa cv. Nipponbare (a recurrent parent) and O. rufipogon acc. W630 (a donor parent). As a result, two strong QTLs were detected on chromosomes 1 and 4, and they were found to be identical to the two major seed-shattering loci, qSH1 and sh4, respectively. The allelic interaction at these loci was further examined using two sets of backcross populations having reciprocal genetic backgrounds, cultivated and wild. In the genetic background of cultivated rice, the wild qSH1 allele has stronger effect on seed shattering than that of sh4. In addition, the wild alleles at both qSH1 and sh4 loci showed semi-dominant effects. On the other hand, in the genetic background of wild rice, non-shattering effects of Nipponbare alleles at both loci were examined to inspect rice domestication from a viewpoint of seed shattering. It was serendipitous that the backcross plants individually having Nipponbare homozygous alleles at either shattering locus (qSH1 or sh4) shed all the seeds. This fact strongly indicates that the non-shattering behavior was not obtained by a single mutation in the genetic background of wild rice. Probably, some other minor genes are still associated with the formation or activation of abscission layer, which enhance the seed shattering.

    GENETICS SOC JAPAN, 2010年08月, GENES & GENETIC SYSTEMS, 85 (4), 265 - 271, 英語

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    研究論文(学術雑誌)

  • Genetic diversity and origin of timopheevi wheat inferred by chloroplast DNA fingerprinting

    Naoki Mori, Yasutaka Kondo, Takashige Ishii, Taihachi Kawahara, Jan Valkoun, Chiharu Nakamura

    Domesticated timopheevi wheat (Triticum timopheevi) is an endemic crop of western Georgia in Transcaucasia. It has a distinct nuclear genome (2n=28, AAGG) and is genetically isolated from other wheat species. To clarify the genetic diversity and the domestication of this interesting wheat, we analyzed molecular variation at 23 microsatellite loci in the chloroplast genome. Allelic diversity was evaluated using 94 accessions representing domesticated timopheevi wheat (T. timopheevi), wild timopheevi wheat (T. araraticum), and wild emmer wheat (T. dicoccoides). The average diversity index (H) in T araraticum (0.206) was smaller than that in T. dicoccoides (0.284). No polymorphisms were detected among the six accessions of T. timopheevi, suggested a monophyletic origin of domesticated timopheevi wheat. Phylogenetic analyses of the plastotypes revealed clear differences between the chloroplast DNA of timopheevi wheat and emmer wheat, and thus supported the hypothesis that these two wheat species originated independently. None of the T. araraticum plastotypes collected in Transcaucasia were closely related to the T. timopheevi plastotype. On the other hand, the plastotypes found in northern Syria and southern Turkey showed closer relationships with T. timopheevi. These results suggested that the domestication of timopheevi wheat might have occurred in the region including southern Turkey and northern Syria.

    JAPANESE SOC BREEDING, 2009年12月, BREEDING SCIENCE, 59 (5), 571 - 578, 英語

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    研究論文(学術雑誌)

  • Evaluation of genetic diversity among wild rice populations of Oryza rufipogon in Myanmar and Cambodia

    Kageyama H, Akimoto M, Shishido R, Sein T, Htut T, Sarom M, Sato T, Ishii T

    2009年, J Crop Res, 54, 47 - 51, 英語

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    研究論文(学術雑誌)

  • Evaluation of genetic diversity among rice local varieties in Vietnam

    Takashi Hiraoka, Nobuyuki Ikeda, Ryo Ishikawa, Takashige Ishii

    2009年, Journal of Crop Research, 54, 125 - 130, 英語

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    研究論文(学術雑誌)

  • Mapping of quantitative trait loci associated with cold tolerance at the post-germination stage in rice

    A. L. Ranawake, T. Ishii, N. Mori, S. Yoshida, C. Nakamura

    Cold tolerance at the post-germination stage in rice was studied using gained shoot length (GSL) as a new parameter which was measured as increased shoot length during cold stress at 4 degrees C followed by a 4-day recovery period at 35 T. GSL decreased until 4(th) day in both japonica and indica cultivar while a marked increase occurred in a japonica cultivar after 5 days of cold stress, indicating its higher ability of cold acclimation. Quantitative trait loci (QTLs) associated with cold tolerance were identified using recombinant inbred lines derived from these parents on a linkage map constructed with 102 simple sequence repeat (SSR) markers. By the interval analysis, one and five putative QTLs were identified after 5 and 11 days of cold stress, respectively. The single point analysis indicated that these QTLs explained 16% and 54% of the phenotypic variance after 5 and 11 days of cold stress, respectively. A cold tolerant japonica parent contributed all QTLs, among which one on the long arm of chromosome 7 was common for the two cold stress periods. Based on gained root length one QTL was identified on chromosome 12 explaining 11% of the variance.

    DIAGNOSIS PRESS LTD, 2008年02月, BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 22 (1), 536 - 540, 英語

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    研究論文(学術雑誌)

  • The role of alternating culture temperatures and maltose in enhancing the anther culture efficiency of salt tolerant indica rice (Oryza sativa L.) cultivars, Pokkali and Nona Bokra

    Muhammad A. Javed, Takashige Ishii, Osamu Kamijima, Shuji Misoo

    The effects of different culture temperatures, culture media and saccharides on the anther culture efficiency in two salt tolerant rice (Oryza sativa ssp. indica) cultivars, Pokkali and Nona Bokra, were examined. Comparison of two culture temperatures (constant temperature at 25 degrees C and alternating temperatures at 30 degrees C/20 degrees C) indicated that alternating temperatures showed remarkable effects not only on the callus induction but also on the plant regeneration. Among the basal media examined, higher callus productivity was observed in SK-I medium and higher shoot productivity was obtained in SK-II medium than other basal media. When alternating temperatures and revised N-6 medium including maltose were used throughout anther culture, callus productivity and green shoot productivity showed the highest score in the salt tolerant two cultivars used in this study. Maltose and alternating temperatures increases the anther culture efficiency of recalcitrant indica rice cultivars. This is the first report suggesting high production of haploids or doubled haploids from anther culture of these salt tolerant indica cultivars.

    JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, 2007年06月, PLANT BIOTECHNOLOGY, 24 (3), 283 - 287, 英語

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    研究論文(学術雑誌)

  • Sujay Rakshit, Arunita Rakshit, Hideo Matsumura, Yoshihiro Takahashi, Yoshitaka Hasegawa, Akiko Ito, Takashige Ishii, Naohiko T. Miyashita, Ryohei Terauchi

    Polymorphism over similar to 26 kb of DNA sequence spanning 22 loci and one region distributed on chromosomes 1, 2, 3 and 4 was studied in 30 accessions of cultivated rice, Oryza sativa, and its wild relatives. Phylogenetic analysis using all the DNA sequences suggested that O. sativa ssp. indica and ssp. japonica were independently domesticated from a wild species O. rufipogon. O. sativa ssp. indica contained substantial genetic diversity (pi = 0.0024), whereas ssp. japonica exhibited extremely low nucleotide diversity (pi = 0.0001) suggesting the origin of the latter from a small number of founders. O. sativa ssp. japonica contained a larger number of derived and fixed non-synonymous substitutions as compared to ssp. indica. Nucleotide diversity and genealogical history substantially varied across the 22 loci. A locus, RLD15 on chromosome 2, showed a distinct genealogy with ssp. japonica sequences distantly separated from those of O. rufipogon and O. sativa ssp. indica. Linkage disequilibrium (LD) was analyzed in two different regions. LD in O. rufipogon decays within 5 kb, whereas it extends to similar to 50 kb in O. sativa ssp. indica.

    SPRINGER, 2007年02月, THEORETICAL AND APPLIED GENETICS, 114 (4), 731 - 743, 英語

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    研究論文(学術雑誌)

  • Noriaki Aoki, Takayuki Umemoto, Shinya Yoshida, Takashige Ishii, Osamu Kamijima, Ushio Matsukura, Naoyoshi Inouchi

    The amount of long chains (LC) of amylopectin in high-amylose rice is thought to be one of the important determinants of its quality when cooked. A wide range of differences in LC content have been reported in rice varieties, which can be clearly divided into four classes based on LC and apparent amylose content: namely, amylose and LC-free, low or medium-amylose and low-LC, high-amylose and medium-LC, high-amylose and high-LC. However, genetic factors controlling LC content have not been fully understood. Here, we performed quantitative trait loci (QTL) analysis of LC content using 157 recombinant inbred lines (RILs) derived from a cross of a low-LC cultivar, Hyogokitanishiki, and a high-LC line, Hokuriku 142. By analyzing randomly selected 15 RILs, it was shown that high LC content (>= 11%) was associated with high setback viscosity (>= 200 RVU), and that low LC (>= 3%) was associated with low setback viscosity (>= 130 RVU), as measured by a Rapid Visco Analyzer. With setback viscosity as an indicator for LC content, QTL analysis was conducted using 60 DNA markers including a CAPS marker that distinguished Wx a and Wx b alleles coding for granule-bound starch synthase I (GBSSI or Wx protein), the enzyme working for amylose biosynthesis. Only one QTL with a peak log of likelihood score at the wx locus was detected, and no line showing setback viscosity corresponding to the medium-LC class appeared. The fact that wx mutants of Hokuriku 142 lacked LC in their rice starch supports the view that the functional Wx allele is indispensable for LC synthesis in addition to amylose synthesis in rice endosperm. We suggest three possible reasons why no line with medium-LC content was observed. First, the locus (loci) responsible for generation of medium-LC may be located very close to the wx locus and not able to be dissected by the population and DNA markers we used. Second, there may be special QTLs for medium-LC cultivars that do not exist in low- or high-LC cultivars. Third, medium-LC cultivars may have an as-yet unidentified Wx allele with lower capability in LC synthesis compared to the Wx allele in high-LC cultivars.

    SPRINGER, 2006年09月, EUPHYTICA, 151 (2), 225 - 234, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mitochondrial microsatellite variability in common wheat and its ancestral species

    Takashige Ishii, Chisa Takahashi, Nobuyuki Ikeda, Osamu Kamijima, Naoki Mori

    On the basis of the entire mitochondrial DNA sequence of common wheat, Triticum aestivum, 21 mitochondrial microsatellite loci having more than ten mononucleotide repeats were identified. The mitochondrial microsatellite variability at all loci was examined with 43 accessions from 11 Triticum and Aegilops species involved in wheat polyploidy evolution. Polymorphic banding patterns were obtained at 15 out of 21 mitochondrial microsatellite loci. The number of alleles per polymorphic microsatellite ranged from 2 to 5 with an average of 3.07, and the diversity values (H) ranged from 0.09 to 0.50 with an average of 0.29. These values are almost two third of wheat chloroplast microsatellite values, indicating that variability of mitochondrial microsatellite is much less than that of chloroplast microsatellite. Based on the allele variation at all loci, a total of seven mitochondrial haplotypes were identified among common wheat and its ancestral species. Three diploid species showed their own specific haplotypes and timopheevi group (11 accessions) had three types, whereas 29 accessions of emmer and common wheat groups shared the same haplotype. These results indicate that a single mitochondrial haplotype determined by microsatellite analysis has conservatively been maintained in the evolutionary lineage from wild tetraploid to cultivated hexaploid species.

    GENETICS SOC JAPAN, 2006年06月, GENES & GENETIC SYSTEMS, 81 (3), 211 - 214, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 野生イネの種子脱粒性、穂の開帳性、頴の色を支配する遺伝子のマッピング

    石井 尊生, 崎山 弘樹, 石川 亮, 上島 脩志

    2006年, 近畿作育研究, 51, 23 - 26, 日本語

    [査読有り]

    研究論文(学術雑誌)

  • Discrepancy of two ecotypes of Oryza sativa L. to salinity at germination and seedling stage

    Javed MA, 石井 尊生, Kamijima O, Misoo S

    2006年, Annals Biol, 22, 201 - 211, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mechanism of high photosynthetic capacity in BC2F4 lines derived from a cross between Oryza sativa and wild relatives O-rufipogon

    C Masumoto, T Ishii, T Hatanaka, N Uchida

    We found that several BC2F4 lines had high leaf photosynthetic rates Under light-saturated and ambient CO2 conditions. These lines are progenies of BC,F, plants with high photosynthetic capacities which were generated by backcrossing between Oryza rufipogon (W630) and O. saliva cv. Nipponbare, as a recurrent parent. Some photosynthetic characteristics of the BC2F4, lines were investigated to identify the factors increasing photosynthetic rates. Photosynthetic rates of these lines under light-saturated conditions at 50 to 700 ppm CO2 concentrations were higher than those in Nipponbare. The estimated-maximum photosynthetic rates under light-saturated and CO2-saturated conditions in BC2F4 lines were also higher than that in Nipponbare. The photosynthetic rate under light-saturated and ambient CO2 conditions was positively correlated with the carboxylation efficiency as an indicator of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity in vivo rather than stomatal conductance. Initial and total Rubisco activities in vitro tended to be higher in the BC2F4 lines than in Nipponbare. The content of active Rubisco calculated from the activation state of Rubisco was also higher in the BC2F4 lines than in Nipponbare. These results suggest that high photosynthetic capacities of BC2F1 plants can be maintained high in their progenies and high photosynthetic rates Under light-saturated and ambient CO2 conditions in the BC2F4 lines are achieved mainly by the high activity of Rubisco due to the high active Rubisco content.

    CROP SCIENCE SOC JAPAN, 2005年12月, PLANT PRODUCTION SCIENCE, 8 (5), 539 - 545, 英語

    [査読有り]

    研究論文(学術雑誌)

  • DNA variation in the metallothionein genes in wild rice Oryza rufipogon: Relationship between DNA sequence polymorphism, codon bias and gene expression

    NT Miyashita, K Yoshida, T Ishii

    This study examines the relationship between DNA sequence variation and level of gene expression in four metallothionein genes from wild rice Oryza rufipogon. The nucleotide diversity was 0.0028 to 0.0117 over the entire coding and non-coding region, and it was negatively correlated with gene expression for three type 2 metallothionein genes. In contrast, codon bias and percent of preferred codons correlated positively with gene expression. These results indicate that the intensity of natural selection depends on the level of gene expression, which in turn shapes the level of nucleotide polymorphism. In addition, significant linkage disequilibria were frequent between the metallothionein genes, although significance was not confirmed after multiple test correction. This result suggests that metallothionein genes expressed at different levels are epistatic with respect to fitness, and that gene expression is an important factor determining level of DNA polymorphism.

    GENETICS SOC JAPAN, 2005年06月, GENES & GENETIC SYSTEMS, 80 (3), 173 - 183, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Development of near isogenic lines targeting wild QTL from Oryza rufipogon in the genetic cackground of O.sativa by marker-assisted selection and backcrossing.

    IKEDA N, KAMIJIMA Osamu, ISHII Takashige

    2005年, Mem. Grad. Sci.Technol.,Kobe Univ., 23-A:37-49, 英語

    [査読有り]

    研究論文(学術雑誌)

  • C Vitte, T Ishii, F Lamy, D Brar, O Panaud

    The origin of rice domestication has been the subject of debate for several decades. We have compared the transpositional history of 110 LTR retrotransposons in the genomes of two rice varieties, Nipponbare (Japonica type) and 93-11 (Indica type) whose complete sequences have recently been released. Using a genomic paleontology approach, we estimate that these two genomes diverged from one another at least 200,000 years ago, i.e., at a time which is clearly older than the date of domestication of the crop (10,000 years ago, during the late Neolithic). In addition, we complement and confirm this first in silico analysis with a survey of insertion polymorphisms in a wide range of traditional rice varieties of both Indica and Japonica types. These experimental data provide additional evidence for the proposal that Indica and Japonica rice arose from two independent domestication events in Asia.

    SPRINGER, 2004年12月, MOLECULAR GENETICS AND GENOMICS, 272 (5), 504 - 511, 英語

    [査読有り]

    研究論文(学術雑誌)

  • K Yoshida, NT Miyashita, T Ishii

    Nucleotide variation in the alcohol dehydrogenase (Adh1) locus region of the wild rice Oryza rufipogon and its related species was analysed to clarify the maintenance mechanism of DNA variation in Oryza species. The estimated nucleotide diversity in the Adh1 locus region of O. rufipogon was 0.002, which was one of the lowest values detected in nuclear loci of plant species investigated so far. Tests of neutrality detected significantly negative deviation from the neutral mutation model for the coding region, especially for replacement sites. When each of the ADH1 domains was considered, significance was detected only for the catalytic domain 1. These results suggest purifying selection in the Adh1 coding region. In the phylogenetic tree of Oryza species based on Adh1 variation, cultivated rice O. sativa subspp. japonica and indica were included in the cluster of O. rufipogon. The genetic distance of the Adh1 region between O. rufipogon and O. sativa was as low as the nucleotide diversity of O. rufipogon. These results imply that O. rufipogon and O. sativa cannot be classified based on the nucleotide variation of Adh1. No replacement divergence between O. rufipogon and the other three A-genome species (O. glumaepatula, O. barthii and O. meridionalis) were detected, indicating that ADH1 is conserved in the A-genome species. On the other hand, between O. rufipogon and the E-genome species O. australiensis, replacement changes were detected only in the catalytic domain 1. The difference in replacement substitutions between the A- and E-genome species may be related to adaptive changes in the ADH1 domains, reflecting environmental differences where the species encounter anaerobic stress.

    SPRINGER, 2004年11月, THEORETICAL AND APPLIED GENETICS, 109 (7), 1406 - 1416, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Z Hashimoto, N Mori, M Kawamura, T Ishii, S Yoshida, M Ikegami, S Takumi, C Nakamura

    Japanese rice (Oryza sativa L.) cultivars that are strictly used for the brewing of sake (Japanese rice wine) represent a unique and traditional group. These cultivars are characterized by common traits such as large grain size with low protein content and a large, central white-core structure. To understand the genetic diversity and phylogenetic characteristics of sake-brewing rice, we performed amplified fragment length polymorphism and simple sequence repeat analyses, using 95 cultivars of local and modern sake-brewing rice together with 76 cultivars of local and modern cooking rice. Our analysis of both nuclear and chloroplast genome polymorphisms showed that the genetic diversity in sake-brewing rice cultivars was much smaller than the diversity found in cooking rice cultivars. Interestingly, the genetic diversity within the modern sake-brewing cultivars was about twofold higher than the diversity within the local sake-brewing cultivars, which was in contrast to the cooking cultivars. This is most likely due to introgression of the modern cooking cultivars into the modern sake-brewing cultivars through breeding practices. Cluster analysis and chloroplast haplotype analysis suggested that the local sake-brewing cultivars originated monophyletically in the western regions of Japan. Analysis of variance tests showed that several markers were significantly associated with sake-brewing traits, particularly with the large white-core structure.

    SPRINGER, 2004年11月, THEORETICAL AND APPLIED GENETICS, 109 (8), 1586 - 1596, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Chloroplast and nuclear DNA variation in common wheat: insight into the origin and evolution of common wheat

    S Hirosawa, S Takumi, T Ishii, T Kawahara, C Nakamura, N Mori

    To understand the origin and evolution of common wheat, chloroplast (ct) and nuclear DNA variations were studied in five hexaploid and three tetraploid wheat subspecies. Based on chloroplast simple sequence repeats at 24 loci, they were classified into two major plastogroups. Plastogroup I consisted of 11 plastotypes, including the major plastotype H10 that occurred at the highest frequency (59%) in common wheat. Plastogroup 11 consisted of five plastotypes and occurred in eight out of 27 accessions of T aestivum ssp. spelta and one accession of ssp. aestivum. As for nuclear DNA variations, AFLP data using 10 primer sets revealed two major clades of a phylogenetic tree constructed by UPGMA (unweighted pair-group method with arithmetic mean), one consisting of common wheat and the other of emmer wheat. The clade of common wheat was further divided into two major and six minor subclades. One of the major subclades consisted only of non-free-threshing ssp. spelta accessions, which were grouped into two clusters, one consisting only of accessions with plastogroup I ctDNA and the other with both plastogroups I and IT. T. aestivum ssp. macha, another non-free-threshing common wheat, formed the other cluster. Taken together, our data indicate the existence of at least two maternal lineages in common wheat and support the hypothesis that European spela wheat originated in Europe separately from other groups of common wheat.

    GENETICS SOC JAPAN, 2004年10月, GENES & GENETIC SYSTEMS, 79 (5), 271 - 282, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Enhancement of rice leaf photosynthesis by crossing between cultivated rice, Oryza sativa and wild rice species, Oryza ryfipogon

    C Masumoto, T Ishii, S Kataoka, T Hatanaka, N Uchida

    To study whether wild rice species have genes that may increase potential photosynthetic capacities of rice cultivars, we generated BC, populations by reciprocally backcrossing Oryza rufipogon (W630) with O. sativa cv. Nipponbare and IR36; N-BC2 populations and IR-BC2 populations, respectively. We measured the oxygen evolution rates (OER) of single leaves under saturating light and CO2 as the maximum photosynthetic rates and the contents of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase. Several lines in each BC, population had significantly higher OERs than parental cultivars, and 14similar to25% of plants in BC2 populations had higher OERs than the highest values in parental cultivars. The highest OERs in BC2 populations were about 60% higher than average OERs in parental cultivars. The BC2 populations contained 30-40% more Rubisco than parental cultivars. The Rubisco activase contents in N-BC2 populations were 15-30% lower than that in Nipponbare. Cytoplasms derived from O. rufipogon and O. sativa had different effects on the contents of Rubisco and Rubisco activase particularly in N-BC2 populations. In several lines of each BC2 population the OERs had positive correlations with the contents of Rubisco and/or Rubisco activase. These results suggest that O. rufipogon can be used as a source of germplasm to enhance the photosynthetic capacity of O. sativa.

    CROP SCIENCE SOC JAPAN, 2004年09月, PLANT PRODUCTION SCIENCE, 7 (3), 252 - 259, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Wild-QLT-allele effect in the background of Japonica (Nipponbare) and Indica (IR36) cultivars.

    ISHII Takashige, BAUTISTA N S, SHIMADZUTSU K, KOBAYASHI N, UCHIDA Naotsugu, KAMIJIMA Osamu

    2003年, Advances in Rice Genetics, pp.133-135, 英語

    研究論文(学術雑誌)

  • Trait-improving wild QTL alleles identified using advanced backcross QLT analysis from a cross between cultivated rice, Oryza sativa and wild rice, O. rufipogon.

    BAUTISTA N S, SHIMADZUTSU K, TERANISHI T, TAKAMATSU S, KOBAYASHI N, UCHIDA Naotsugu, KAMIJIMA Osamu, ISHII Takashige

    2003年, Advances in Rice Genetics, pp.135-138, 英語

    研究論文(学術雑誌)

  • Salinity tolerance of three Basmati rice lines: A comparative study of yield related characters.

    JAVED M. A, SHABBIR G, MISOO Shuji, ISHII Takashige, KAMIJIMA Osamu

    2003年, Kinki J. Crop Sci. Breed., 48,pp.7-12., 英語

    研究論文(学術雑誌)

  • Marker-assisted evaluation of germplasm resources for plant breeding

    Xu Y, Ishii T, McCouch SR

    International Rice Research Institute, 2003年, Rice Science: Innovations and Impact for Livelihood, 213 - 229, 英語

    [査読有り]

    研究論文(学術雑誌)

  • QTL analysis for plant and grain characters of sake-brewing rice using a doubled haploid population

    S Yoshida, M Ikegami, J Kuze, K Sawada, Z Hashimoto, T Ishii, C Nakamura, O Kamijima

    Rice (Oryza sativa L.) varieties used for brewing sake are commonly characterized by traits such as large grain size with white-core (an opaque structure inside the rice grain). A linkage map was constructed using doubled haploid lines derived from the cross of Reiho (a cooking variety) and Yamada-nishiki (a sake-brewing variety). Random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker systems were employed in QTL analysis. A total of 145 markers were identified and mapped on rice chromosomes. QTLs for plant and grain characters were detected by interval mapping and single point analysis. Several QTLs with a significant contribution were identified for important sake-brewing characters including grain size, grain shape, white-core grain rate and protein content. Several QTLs simultaneously affected the grain weight, width and thickness, while QTLs for the grain length independently affected the grain size. QTLs for the white-core grain rate did not affect the grain size, although one QTL for the white-belly grain rate simultaneously affected the grain weight, width and thickness. Several QTLs were detected for the protein content in both brown and polished rice. One QTL on chromosome 4 that was effective for the decrease of the protein content in polished rice showed a positive relation with the grain length. One QTL with the largest effect on the grain length on chromosome 11 did not contribute to the decrease of the protein content in polished rice. Therefore, it is suggested that the grain length QTL on chromosome 4 might control not only the grain shape but also the internal structure related to the milling efficiency and/or location of the storage protein.

    JAPANESE SOC BREEDING, 2002年12月, BREEDING SCIENCE, 52 (4), 309 - 317, 英語

    [査読有り]

    研究論文(学術雑誌)

  • N Asakura, C Nakamura, T Ishii, Y Kasai, S Yoshida

    Two Mu-like transposable elements were cloned from a rice genomic library using a partial cDNA clone that exhibits high homology to the mudrA gene of the maize element MuDR. Database searches led to the identification of six other sequences that carried highly homologous terminal inverted repeats (TIRs). All the rice elements possessed similar to200-by TIRs, and four were flanked by 9-bp target-site duplications (TSDs). The longer of the two cloned elements, OsMu4-2, could potentially encode a protein colinear with a MURA-like transposase, but it had stop codons in the coding region indicating that it is a pseudogene. All the other elements had large internal deletions. Direct dinucleotide repeats were found in two elements at positions flanking the deleted regions, suggesting that the deletions arose via the interrupted-gap-repair mechanism. Sequences related to empty sites of insertion were found in OsMu4-2 and one of the elements identified in the databases. These results provide evidence that the rice OsMu element was active and transposed in the past. Analysis of OsMu4-2 cDNAs revealed two types of transcripts produced by alternative splicing. Genomic Southern analysis suggested that OsMu4-2 was conserved in rice species with the A genome, but a deleted version was unique to japonica subspecies. Some wild rice species harbored paralogous copies of the OsMu element.

    SPRINGER-VERLAG, 2002年11月, MOLECULAR GENETICS AND GENOMICS, 268 (3), 321 - 330, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 農業形質から評価した在来アズキ系統の遺伝的多様性

    伊勢村武久, 石井尊生, 三十尾修司, 上島脩志

    2002年, 神戸大学大学院自然科学研究科紀要, 20-B, 165 - 176, 日本語

    研究論文(学術雑誌)

  • RAPD分析により評価した在来アズキ系統の遺伝的多様性

    伊勢村武久, 石井尊生, 斎藤大樹, 野田千代, 三十尾修司, 上島脩志

    2002年, 育種学研究, 4, 125 - 135, 日本語

    [査読有り]

    研究論文(大学,研究機関等紀要)

  • Construction of molecular linkage map between Oryza sativa and O. rufipogon, using microsatellite markers

    Ishii T, Takamatsu S, Ito H, Ikeda N, Isemura T, Kamijima O

    2002年, Rice Genet Newsl, 19, 97 - 100, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Evaluation of allelic diversity at chloroplast microsatellite loci among common wheat and its ancestral species

    T Ishii, N Mori, Y Ogihara

    Twenty four chloroplast microsatellite loci having more than ten mononucleotide repeats were identified from the entire chloroplast DNA sequence of common wheat, Triticum aestivum cv Chinese Spring. For each microsatellite, a pair of primers were designed to produce specific PCR products in the range of 100-200 bp. The allelic diversity at the microsatellite loci was evaluated using 43 accessions from 11 Triticum and Aegilops species involved in wheat polyploid evolution. Polymorphic banding patterns were obtained at 21 out of 24 chloroplast microsatellite loci. The three monomorphic microsatellites were found to be located in coding regions. For the polymorphic microsatellites, the number of alleles per microsatellite ranged from 2 to 7 with an average of 4.33. and the diversity values (H) ranged from 0.05 to 0.72 with an average of 0.47. Significant correlations (P<0.01) were observed between the number of repeats and the number of alleles, and between the number of repeats and diversity value, respectively. The genetic diversity explained by chloroplast microsatellites and nuclear RFLP markers were compared using 22 tetraploid accessions. Although the number of alleles for nuclear RFLP markers was found to be higher than that for chloroplast microsatellites. similar diversity values were observed for both types of markers. Among common wheat and its ancestral species, the percentages of common chloroplast microsatellite alleles were calculated to examine their phylogenetic relationships. As a result, Timopheevi wheat species were clearly distinguished from other species, and Emmer and common wheat species were divided into two main groups, each consisting of a series of wild and cultivated species from tetraploid to hexaploid. This indicates that the two types of chloroplast genomes of common wheat might have independently originated from the corresponding types of wild and cultivated Emmer wheat species.

    SPRINGER-VERLAG, 2001年11月, THEORETICAL AND APPLIED GENETICS, 103 (6-7), 896 - 904, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Nuclear- and chloroplast-microsatellite variation in A-genome species of rice

    T Ishii, Y Xu, McCouch, SR

    Simple sequence length polymorphism analysis was carried out to reveal microsatellite variation and to clarify the phylogenetic relationships among A-genome species of rice. Total DNA from 29 cultivars (23 Oryza sativa and 6 O. glaberrima) and 30 accessions of wild A-genome species (12 O. rufipogon, 5 O. glumaepatula, 2 O. longistaminata, 6 O. meridionalis, and 5 O. barthii) was used as a template for PCR to detect 24 nuclear and 10 chloroplast microsatellite loci. Microsatellite allelic diversity was examined based on amplified banding patterns. Microsatellites amplified clearly in all 59 accessions, with an average of 18.4 alleles per locus. The polymorphism information content (PIC) value ranged from 0.85 to 0.94, with an average of 0.89. At the species level, high average PIC values were observed in O. sativa (0.79) and O. rufipogon (0.80). For chloroplast microsatellites, the average number of alleles per locus and the average PIC value were 2.9 and 0.38, respectively. While the magnitude of diversity was much greater for nuclear microsatellites than for chloroplast microsatellites, they showed parallel patterns of differentiation for each taxonomic group. Using the ratio of common alleles (estimated as size of amplified fragments) as a similarity index, the average percentages of common microsatellite alleles were calculated between taxa. For both nuclear and chloroplast microsatellites, O. sativa showed the highest similarity values to O. rufipogon, and O. glaberrima was most similar to O. barthii. These data support previous evidence that these cultivars originated from the corresponding wild ancestral species.

    NATL RESEARCH COUNCIL CANADA, 2001年08月, GENOME, 44 (4), 658 - 666, 英語

    [査読有り]

    研究論文(学術雑誌)

  • RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice

    NS Bautista, R Solis, O Kamijima, T Ishii

    RAPD, RFLP, nuclear SSLP and chloroplast SSLP analyses were carried out to clarify the phylogenetic relationships among A-genome species of rice. In total, 12 cultivars of Oryza sativa (4 Japonica, 3 Javanica and 5 Indica), one cultivar of O. glaberrima, and 17 wild accessions (12 O. rufipogon, 2 O. glumaepatula, 1 O. longistaminata, 1 O. meridionalis and 1 O. barthii) were used. Their banding patterns were scored and compared to evaluate the similarity between accessions. Genetic differentiation within and between taxa was examined based on the average similarity indices. Except for chloroplast SSLP analysis, the average similarities were higher within O. saliva than within O. rufipogon, and O. saliva Indica had greater intrasubspecific variation than Japonica and Javanica. Comparisons between cultivated and wild species showed that O. saliva was closely related to O. rufipogon, while O. glaberrima was closely related to O. barthii. This indicated that two cultivated species, O. saliva and O. glaberrimia, originated from O. rufipogon and O. barthii, respectively. Domestication of O. saliva seemed to be diphyletic, since strong similarity was observed between O. saliva Japonica-Javanica and O. rufipogon from China and between (3. saliva Indica and O. rufipogon from tropical Asia. In addition, dendrograms for RAPD, RFLP, and nuclear and chloroplast SSLP analyses were constructed to reveal the overall genetic relationships among A-genome species. In all analyses, O. saliva and O. glaberrima formed groups with O. rufipogon and O. barthii, respectively. However, their manners of clustering with other wild species were not the same. The results of RAPD and RFLP analyses indicate that O. glumaepatula was relatively close to the groups of O. saliva and O. glaberrima whereas O. longistaminata and O. meridionalis were highly differentiated from other A-genome species. On the other hand, clear interspecific relationships were not obtained by nuclear or chloroplast SSLP analyses.

    GENETICS SOC JAPAN, 2001年04月, GENES & GENETIC SYSTEMS, 76 (2), 71 - 79, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Ultra-simple DNA extraction method for marker-assisted selection using microsatellite markers in rice

    N Ikeda, NS Bautista, T Yamada, O Kamijima, T Ishii

    We present an ultra-simple DNA extraction method for microsatellite analysis of rice. Each extraction requires only one microtube, one disposable pipette tip, TE buffer and few pieces (about 5 mm) of rice leaf tissue. This is sufficient for 200 PCR reactions. The extract can be kept in the freezer for long-term storage. Also, DNA can be extracted from 200-300 individuals in a few hours. These features enabled us to perform rapid large-scale seedling genotyping required for marker-assisted selection. We have also examined the applicability of this method for other PCR-based markers: RAPDs, nuclear STS, chloroplast STS and chloroplast microsatellites.

    INT SOC PLANT MOLECULAR BIOLOGY, 2001年03月, PLANT MOLECULAR BIOLOGY REPORTER, 19 (1), 27 - 32, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Assignment of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph4 to the rice chromosome 6

    M Kawaguchi, K Murata, T Ishii, S Takumi, N Mori, C Nakamura

    A Sri Lankan indica rice (Oryza sativa L.) cultivar Babawee harboring a brown planthopper (BPH) (Nilaparvata lugens Stal) resistance gene bph4 was crossed as a male parent with two susceptible cultivars, either indica IR24 or japonica Tsukushibare. Segregation of the BPH resistance in the two crosses was studied by directly assaying the F-2 phenotypes and by determining the F-2 genotypes based on the F-3 phenotypes, In both cross combinations, the segregation of the BPH resistance significantly deviated from the ratio expected for the single recessive gene model, Using bulked DNAs of homozygous resistant and susceptible F(2)s and the parents, seven out of 214 RFLP markers were selected as linked markers, of which sis on chromosome 6 mere common in the two cross combinations, Two microsatellite markers on chromosome 6 were also linked to bph4, Although the map position of bph4 could not be determined, the gene was assigned to the distal region of the short arm of rice chromosome 6 based on the bulked segregant analysis and linkage analysis.

    JAPANESE SOC BREEDING, 2001年03月, BREEDING SCIENCE, 51 (1), 13 - 18, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Microsatellites and microsynteny in the chloroplast genomes of Oryza and eight other Gramineae species

    T Ishii, McCouch, SR

    Primer pairs flanking ten chloroplast microsatellite loci, originally identified in Oryza sativa cv Nipponbare, were evaluated for amplification and allelic diversity using a panel of 13 diverse cultivars of rice (O. sativa), 19 accessions of wild rice (three O. officinalis, five O. latifolia, five O. minuta, four O. australiensis, one O. brachyantha and one O, ridleyi) and eight other Gramineae species (maize, teosinte, wheat, oat, barley? pearl millet, sorghum and sugarcane). Amplified products were obtained for all samples at nine out of ten loci. Among the rice cultivars, the number of alleles per locus ranged from one to four, with monomorphic patterns observed at five loci. The average polymorphism information content (PIC) value at the other five (polymorphic) loci was 0.54 among the 13 cultivars. When wild rice and the other Gramineae species were compared based on the proportion of shared alleles, their phylogenetic relationships were in agreement with previous studies using different types of markers; however, the magnitude of the differences based on chloroplast microsatellites underestimated the genetic distance separating these divergent species and genera. A sequence based comparison of homologous regions of the rice and maize chloroplast genomes revealed that, while a high level of microsynteny is evident, the occurrence of actively evolving microsatellite motifs in specific regions of the I-ice chloroplast genome appears to be mainly a species or genome-specific phenomenon. Thus the chloroplast primer pairs used in this study bracketed mutationally active microsatellite motifs in rice but degenerate, interrupted motifs or highly conserved, mutationally inert motifs in distantly related genera.

    SPRINGER VERLAG, 2000年06月, THEORETICAL AND APPLIED GENETICS, 100 (8), 1257 - 1266, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.)

    S Temnykh, WD Park, N Ayres, S Cartinhour, N Hauck, L Lipovich, YG Cho, T Ishii, McCouch, SR

    In order to enhance the resolution of an existing genetic map of rice, and to obtain a comprehensive picture of marker utility and genomic distribution of microsatellites in this important grain species, rice DNA sequences containing simple sequence repeats (SSRs) were extracted from several small-insert genomic libraries and from the database. One hundred and eighty eight new microsatellite markers were developed and evaluated for allelic diversity. The new simple sequence length polymorphisms (SSLPs) were incorporated into the existing map previously containing 124 SSR loci. The 312 microsatellite markers reported here provide whole-genome coverage with an average density of one SSLP per 6 cM. In this study, 26 SSLP markers were identified in published sequences of known genes, 65 were developed based on partial cDNA sequences available in GenBank, and 97 were isolated from ge-nomic libraries. Microsatellite markers with different SSR motifs are relatively uniformly distributed along rice chromosomes regardless of whether they were derived from genomic clones or cDNA sequences. However, the distribution of polymorphism detected by these markers varies between different regions of the genome.

    SPRINGER VERLAG, 2000年03月, THEORETICAL AND APPLIED GENETICS, 100 (5), 697 - 712, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.)

    YG Cho, T Ishii, S Temnykh, Chen, X, L Lipovich, McCouch, SR, WD Park, N Ayres, S Cartinhour

    The growing number of rice microsatellite markers warrants a comprehensive comparison of allelic variability between the markers developed using different methods, with various sequence repeat motifs, and from coding and non-coding portions of the genome. We have performed such a comparison over a set of 323 microsatellite markers; 194 were derived from genomic library screening and 129 were derived from the analysis of rice-expressed sequence tags (ESTs) available in public DNA databases. We have evaluated the frequency of polymorphism between parental pairs of six inter-subspecific crosses and one inter-specific cross widely used for mapping in rice. Microsatellites derived from genomic libraries detected a higher level of polymorphism than those derived from ESTs contained in the GenBank database (83.8% versus 54.0%). Similarly, the other measures of genetic variability [the number of alleles per locus, polymorphism information content (PIC), and allele size ranges] were all higher in genomic library-derived microsatellites than in their EST-database counterparts. The highest overall degree of genetic diversity was seen in GA-containing microsatellites of genomic library origin, while the most conserved markers contained CCG- or CAG-trinucleotide motifs and were developed from GenBank sequences. Preferential location of specific motifs in coding versus non-coding regions of known genes was related to observed levels of microsatellite diversity. A strong positive correlation was observed between the maximum length of a microsatellite motif and the standard deviation of the molecular-weight of amplified fragments. The reliability of molecular weight standard deviation (SDmw) as an indicator of genetic variability of microsatellite loci is discussed.

    SPRINGER VERLAG, 2000年03月, THEORETICAL AND APPLIED GENETICS, 100 (5), 713 - 722, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Comparative molecular mapping in Ceratotropis species using an interspecific cross between azuki bean (Vigna angularis) and rice bean (V-umbellata)

    A Kaga, T Ishii, K Tsukimoto, E Tokoro, O Kamijima

    A genetic linkage map was developed with 86 F-2 plants derived from an interspecific cross between azuki bean (Vigna angularis, 2n=2x=22) and rice bean (V. umbellata, 2n=2x=22). In total, 14 linkage groups, each containing more than 4 markers, were constructed with one phenotypic, 114 RFLP and 74 RAPD markers. The total map size was 1702 cM, and the average distance between markers was 9.7 cM. The loci showing significant deviation from the expected ratio clustered in several linkage groups. Most of the skewed loci were due to the predominance of rice bean alleles. The azuki-rice bean linkage map was compared with other available maps of Vigna species in subgenus Ceratotropis. Based on the lineage of the common mapped markers, 7 and 16 conserved linkage blocks were found in the interspecific map of azuki bean x V. nakashimae and mungbean map, respectively. Although the present map is not fully saturated, it may facilitate gene tagging, QTL mapping and further useful gene transfer for azuki bean breeding.

    SPRINGER VERLAG, 2000年01月, THEORETICAL AND APPLIED GENETICS, 100 (2), 207 - 213, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Mitochondrial genome differentiation in the genus Phyllostachys

    F Taguchi-Shiobara, T Ishii, T Terachi, K Tsunewaki

    Mitochondrial genome differentiation in the genus Phyllostachys was investigated by restriction fragment length polymorphism (RFLP) analyses of the restriction fragment patterns or Southern hybridization patterns of endonuclease-treated mitochondrial (mt) DNA. First, intraspecific variation of mtDNA in 3 species, P. pubescens, P. nigra and P. bambusoides, was studied using a large number of samples collected from different locations in Japan. Little intraspecific differentiation was detected in P. nigra and P. bambusoides, whereas P. pubescens showed some intraspecific variation. Second, the restriction fragment patterns and Southern hybridization patterns of mtDNAs of 13 Phyllostachys species were analyzed. Their comparison indicated that P. nigra and P. dulcis, and P. angusta and P. propinqua have the same mitochondrial genome, respectively. The restriction patterns allowed the identification of 9 out of 13 species. Based on the percentage of common restriction fragments, all the species except for P. aureosulcata were clustered into the following 3 groups by the UPGMA method; (1) P. angusta, P. propinqua, P. pubescens and P. praecox, (2) P. nigra, P. dulcis, P. humilis and P. aurea, and (3) P. bambusoides, P. bissetii, P. viridis and P. makinoi. Clustering of 13 species based on the results of Southern pattern analysis led to the identification of the following 4 groups; (1) P. angusta, P. propinqua, P. aureosulcata, P. pubescens, P. praecox and P. bambusoides, (2) P. nigra and P. dulcis, (3) P. bissetii, P. viridis and P. makinoi, and (4) P. humilis and P. aurea. The results of the 2 methods differed in the following aspects; (1) affiliation of P. bambusoides differed, (2) cluster 2 in the restriction pattern analysis was divided into 2 clusters by Southern pattern analysis, and (3) intercluster relationships were considerably different between the 2 dendrograms. To develop reliable phylogenetic relationships in the genus Phyllostachys, it is necessary to increase the number of restriction enzymes used in the restriction pattern analysis as well as the number of probes used in the Southern pattern analysis.

    TROPICAL AGRICULTURE RES CENTR, 1998年01月, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 32 (1), 7 - 14, 英語

    研究論文(学術雑誌)

  • Hideo Maeda, Takashige Ishii, Hirokazu Mori, Junji Kuroda, Masato Horimoto, Itsuro Takamure, Toshiro Kinoshita, Osamu Kamijima

    High density molecular map of semidwarfing gene, sd-1, was constructed using 178 F2 plants derived from the cross between the semidwarf near-isogrnic line and its recurrent parent, japonica cultivar 'Shiokari'. The total size of the map including nine molecular markers (five RAPD and four RFLP markers) and sd-1 was 6.4 cM. The most closely linked RAPD markers in both sides of sd-1 were OPV10500 and OPAE141000, and the distance between them was 1.7 cM. Since sd-1 is a recessive gene, these closely linked RAPD markers will be very powerful to monitor the gene for large scale selection in breeding program.

    1997年12月, Breeding Science, 47 (4), 317 - 320

    研究論文(学術雑誌)

  • Susan R. McCouch, Xiuli Chen, Olivier Panaud, Svetlana Temnykh, Yunbi Xu, Yong Gu Cho, Ning Huang, Takashige Ishii, Matthew Blair

    Microsatellites are simple, tandemly repeated di- to tetra-nucleotide sequence motifs flanked by unique sequences. They are valuable as genetic markers because they are co-dominant, detect high levels of allelic diversity, and are easily and economically assayed by the polymerase chain reaction (PCR). Results from Screening a rice genomic library suggest that there are an estimated 5700-10 000 microsatellites in rice, with the relative frequency of different repeats decreasing with increasing size of the motif. A map consisting of 120 microsatellite markers demonstrates that they are well distributed throughout the 12 chromosomes of rice. Five multiple copy primer sequences have been identified that could be mapped to independent chromosomal locations. The current level of genome coverage provided by these simple sequence length polymorphisms (SSLPs) in rice is sufficient to be useful for genotype identification, gene and quantitative trait locus (QTL) analysis screening of large insert libraries and marker-assisted selection in breeding. Studies of allelic diversity have documented up to 25 alleles at a single locus in cultivated rice germplasm and provide evidence that amplification in wild relatives of Oryza sativa is generally reliable. The availability of increasing numbers of mapped SSLP markers can be expected to complement existing RFLP and AFLP maps, increasing the power and resolution of genome analysis in rice.

    1997年09月, Plant Molecular Biology, 35 (1-2), 89 - 99

    研究論文(学術雑誌)

  • Tetsuya Yamada, Shuji Misoo, Takashige Ishii, Yasuhiro Ito, Keigo Takaoka, Osamu Kamijima

    Electrofusion was performed to produce somatic hybrids between Solanum tuberosum (2n=4x=48) and dihaploid S. acaule (2n=2x=24). Among the 18 regenerated plants obtained, 14 were found to be hexaploid hybrids based on RAPD analysis and cytological observation. In order to identify their cytoplasmic genome constitution, RFLP analysis of chloroplast and mitochondrial DNA was carried out. Six and eight hybrids were found to have S. tuberosum and S. acaule chloroplast genomes, respectively, suggesting that the chloroplast genomes of the somatic hybrids segregated randomly. In addition, two regenerated plants derived from the same callus had different chloroplast genomes, indicating that the segregation of the chloroplast genome occurred before shoot formation from callus. Concerning the mitochondrial genome constitution, 13 hybrids harbored the genome of S. tuberosum type and the others the S. acaule type genome. This mode of segregation is obviously in favor of S. tuberosum mitochondrial genome. Regarding cytoplasmic genome segregation in somatic hybrids, the mitochondrial genome was not associated with the chloroplast genome. For the cytoplasmic genomes of both species, no hybrid was observed with recombinant or heterogeneous genome from both parents. In most of the somatic hybrids, some characters such as plant habit, shape of foliage and shape of tuber, were generally similar to those of S. tuberosum. The high level of resistance to potato virus X (PVX) detected in dihaploid S. acaule and somatic hybrids, suggests that the resistance to PVX is incorporated by fusion with S. acaule.

    1997年09月, Breeding Science, 47 (3), 229 - 236

    研究論文(学術雑誌)

  • Phylogenetic relationships among rice species based on chloroplast genome differentiation

    Ishii T, Tsunewaki K

    1997年, Science of the Rice Plant, 3, 48 - 53

  • 近縁野生種を用いたバレイショ体細胞雑種のゲノム構成と戻し交雑世代における染色体数の変異

    山田哲也, 中川久美, 保坂和良, 三十尾修司, 石井尊生, 上島脩志

    1997年, 近畿作物・育種研究, 42, 47 - 48

  • バレイショ二倍性半数体間の体細胞雑種系統における細胞質ゲノムの構成

    栗谷由美, 山田哲也, 三十尾修司, 石井尊生, 淡野一郎, 上島脩志

    1997年, 近畿作物・育種研究, 42, 49 - 50

  • A. Kaga, M. Ohnishi, T. Ishii, O. Kamijima

    A genetic linkage map of azuki bean (Vigna angularis) was constructed with molecular and morphological markers using an F2 population of an interspecific cross between azuki bean and its wild relative, V. nakashimae. In total, 132 markers(108 RAPD, 19 RFLP and five morphological markers) were mapped in 14 linkage groups covering 1250 cM; ten remained unlinked. The clusters of markers showing distorted segregation were found in linkage groups 2, 8 and 12. By comparing the azuki linkage map with those of mungbean and cowpea, using 20 RFLP common markers, some sets of the markers were found to belong to the same linkage groups of the respective maps, indicating that these linkage blocks are conserved among the three Vigna species. This map provides a tool for marker-assisted selection and for studies of genome organization in Vigna species.

    1996年10月, Theoretical and Applied Genetics, 93 (5-6), 658 - 663

    研究論文(学術雑誌)

  • Kuldeep Singh, Takashige Ishii, Arnold Parco, Ning Huang, Darshan S. Brar, Gurdev S. Khush

    Rice has become a model cereal plant for molecular genetic research. Rice has the most comprehensive molecular linkage maps with more than 2000 DNA markers and shows synteny and colinearity with the maps of other cereal crops. Until now, however, no information was available about the positions of centromeres and arm locations of markers on the molecular linkage map. Secondary and telotrisomics were used to assign restriction fragment length polymorphism markers to specific chromosome arms and thereby to map the positions of centromeres. More than 170 restriction fragment length polymorphism markers were assigned to specific chromosome arms through gene dosage analysis using the secondary and telotrisomics and the centromere positions were mapped on all 12 linkage groups. The orientations of seven linkage groups were reversed to fit the 'short arm on top' convention and the corrected map is presented.

    1996年06月11日, Proceedings of the National Academy of Sciences of the United States of America, 93 (12), 6163 - 6168

    研究論文(学術雑誌)

  • Phylogenetic relationships between cultivated and wild species of rice as revealed by DNA polymorphisms

    Ishii T, Nakano T, Maeda H, Kamijima O, Khush GS

    1996年, Rice Genetics III, 367 - 372

  • Centromere mapping and orientation of the cytological, classical, and molecular linkage maps of rice

    Khush GS, Singh K, Ishii T, Parco A, Huang N, Brar DS, Multani DS

    1996年, Rice Genetics III, 57 - 75

  • Takashige Ishii, Toshitsugu Nakano, Hideo Maeda, Osamu Kamijima

    In order to clarify the phylogenetic relationships in A-genome species of rice, RAPD analysis was carried out using 29 accessions (8 Oryza sativa, 6 O. glaberrima, 13 O. perennis, and 2 O. breviligulata). Their total DNA was used as template for PCR with 14 decamer primers. The amplified products were electrophoresed in agarose gel, and the banding patterns were observed. Average number of total amplified bands for each accession using 14 primers was 84.6. By comparing the banding patterns, the proportion of common bands between 29 accessions was calculated and a dendrogram showing genetic relationships was constructed. Most of the accessions formed clusters corresponding to their taxonomic grouping. The Oceanian and African forms of O. perennis were substantially different from all other groups. The Asian form of O. perennis, however, formed a complex with O. sativa while O. breviligulata was clustered with O. glaberrima. This indicates that O. sativa and O. glaberrima have probably originated from the Asian form of O. perennis and from O. breviligulata, respectively.

    1996年, Genes and Genetic Systems, 71 (4), 195 - 201

    研究論文(学術雑誌)

  • R. Dalmacio, D. S. Brar, T. Ishii, L. A. Sitch, S. S. Virmani, G. S. Khush

    Most of the commercial hybrids of indica rice are based on wild abortive (WA) source of cytoplasmic-genetic male sterility (CMS). Such cytoplasmic uniformity may lead to genetic vulnerability to disease and insect pests. To overcome this problem, diversification of CMS sources is essential. Crosses of 46 accessions of O. perennis and two accessions of O. rufipogon as female parents were made with two restorers (IR54, IR64) of WA cytosterility. Sterile hybrids were backcrossed with the respective recurrent parents. Of all the backcross derivatives, one line having the cytoplasm of O. perennis Acc 104823 and the nuclear background of IR64 was found to be stable for male sterility. The newly developed CMS line has been designated as IR66707A. This line is completely sterile (0% seed set) under selfed conditions. Crosses of IR66707A with 10 restorers of WA cytoplasm showed almost complete (93-100%) pollen sterility, indicating that the male sterility source of IR66707A is different from WA sterility. Southern hybridization of IR66707A, O. perennis (cytoplasmic donor), IR66707B (maintainer) and V20A (WA cytoplasm) using mitochondrial DNA specific probes (5 endonucleases × 8 probes) showed identical banding patterns between IR66707A and O. perennis. However, in more than half of the combinations, different banding patterns were observed between IR66707A and IR66707B and between IR66707A and V20A. The results suggest that IR66707A has the same cytoplasm as the donor (O. perennis), and CMS may not be caused by any major rearrangement or modification of mtDNA. The new CMS source identified will be useful in cytoplasmic diversification in hybrid rice breeding. © 1995 Kluwer Academic Publishers.

    1995年01月, Euphytica, 82 (3), 221 - 225

    研究論文(学術雑誌)

  • Takashige Ishii, Darshan S. Brar, Gerard Second, Gurdev S. Khush, Koichiro Tsunewaki, Takashige Ishii, Koichiro Tsunewaki

    RFLP analysis was carried out to clarify the nuclear genome differentiation in Asian rice varieties of Oryza saliva. Based on the restriction fragment patterns with two endonucleases, EcoRI and HindIII, using 12 single-copy rice DNA probes, 93 types of nuclear genome were found among 112 local varieties from 17 Asian countries. In a dendrogram showing genetic relationships among nuclear genome types, they were mainly divided into eight groups, A, Bl, B2, CI, C2, Dl, D2 and E. These results were compared with previous isozyme analysis and RFLP analysis on chloroplast genome using the same varieties. Classification on isozyme analysis matches well with that on nuclear genome, indicating synchronous differentiation of isozyme constitutions and nuclear genomes in Asian varieties. Considering the correspondence between them, nuclear genomes were grouped into Indica (A, Bl and B2), intermediate (CI, C2 and Dl) and Japonica (D2 and E) types. From the comparison of chloroplast with nucleus for genome differentiation, two major chloroplast genomes (types 1 and 3) were found in the varieties with several nuclear genome types. However, Japonica group with D2 and E nuclear genomes has only type 1 chloroplast genome, whereas Indica and intermediate groups contain both two major chloroplast genomes. Especially, type 3 chloroplast genome which was not found in Japonica group is dominant type in Indica varieties. The results indicate the differentiation of nuclear genome has partially synchronized with that of chloroplast genome. © 1995, The Genetics Society of Japan. All rights reserved.

    GENETICS SOC JAPAN, 1995年, Japanese Journal of Genetics, 70 (5), 643 - 652, 英語

    研究論文(学術雑誌)

  • T. Ishii, D. S. Brar, D. S. Multani, G. S. Khush

    1994年, Genome, 37 (2), 217 - 221

    研究論文(学術雑誌)

  • T. Ishii, T. Terachi, N. Mori, K. Tsunewaki

    Restriction fragment length polymorphisms of chloroplast (ct), mitochondrial (mt) and nuclear DNA were investigated using eight cultivars of Oryza sativa and two cultivars of O. glaberrima. Relative variability in the nuclear and cytoplasmic genomes was estimated by a common measure, genetic distance. Based on the average genetic distances among ten cultivars for each genome, the evolutionary variabilities of the mitochondrial and nuclear genomes were found to be almost the same, whereas the variability of the chloroplast genome was less than half that of the other two genomes. Cluster analyses on ct and mt DNA variations revealed that chloroplast and mitochondrial genomes were conservative within a taxon and that their differentiations were well-paralleled with respect to each other. For nuclear DNA variation, an array of different degrees of differentiation was observed in O. sativa, in contrast with little variation in O. glaberrima. As a whole, differentiation between O. sativa and O. glaberrima was clearly observed in all three genomes. In O. sativa, no notable difference was found between the cultivars 'Japonica' and 'Javanica', whereas a large differentiation was noticed between 'Japonica' (including 'Javanica') and 'Indica'. In all three genomes, the average genetic distances within 'Indica' were much larger than those within 'Japonica' (including 'Javanica'), and almost similar between 'Japonica' (including 'Javanica') and 'Indica'. These facts indicate that differentiation in O. sativa was due mainly to 'Indica'. © 1993 Springer-Verlag.

    1993年03月, Theoretical and Applied Genetics, 86 (1), 88 - 96

    研究論文(学術雑誌)

  • T. Ishii, K. Tsunewaki

    1991年, Genome, 34 (5), 818 - 826

    研究論文(学術雑誌)

  • CYTOPLASMIC AND NUCLEAR-DNA DIFFERENTIATION IN CULTIVATED RICE SPECIES

    T ISHII, T TERACHI, N MORI, K TSUNEWAKI

    INT RICE RESEARCH INST, 1991年, RICE GENETICS II, 655 - 657, 英語

    研究論文(国際会議プロシーディングス)

  • Takashige Ishii, Olivier Panaud, D. S. Brar, G. S. Khush

    A simple procedure for the detection of rice RFLPs with non-radioactive probes is described. Rice single-copy DNA was labeled with non-radioactive digoxigenin-d UTP. When digested total DNA was hybridized with the non-radioactive labeled DNA probes, RFLPs for rice single-copy DNA could be successfully detected. © 1990 Kluwer Academic Publishers.

    1990年08月, Plant Molecular Biology Reporter, 8 (3), 167 - 171

    研究論文(学術雑誌)

  • Chromosomal location of ribosomal RNA genes in rice by in situ hybridization

    Islam-Faridi MN, Ishii T, Kumar V, Sitch LA, Brar DS

    1990年, Rice Genet Newsl, 7, 143 - 144

    [査読有り]

  • RFLP analysis of rice mitochondrial DNA

    Ishii T, Tsunewaki K

    1989年, Rice Genet Newsl, 6, 153 - 155

  • Takashige Ishii, Toru Terachi, Koichiro Tsunewaki

    Relationships between chloroplast genomes (=ctDNAs) from diploid, Agenome species of rice (Oryza spp.) were established using length differences in restriction fragments of ctDNAs. Five out of 11 endonucleases used revealed differences in ctDNA fragment lengths among 19 accessions of the cultivated species, O. sativa and O. glaberrima. A larger sample of ctDNAs from 66 accessions of both wild and cultivated species were analyzed with three of those five endonucleases, resulting in the identification of nine types of chloroplast genome. In the wild species, the four geographical forms of O. perennis were found to contain one (African), one (Oceanian), three (Asian), and four (American) types, whereas O. breviligulata contained only one type of chloroplast genome. In the cultivated species, the three ecospecies of O. sativa contained one (Japonica and Javanica) and three (Indica) types, whereas O. glaberrima contained one type of chloroplast genome. The latter type was shared exclusively with O. breviligulata, suggesting that the former species had been domesti-cated from the latter. Two chloroplast genome types were shared by O. sativa and the Asian form of O. perennis, suggesting that the former species had derived from the latter via two domestication events. © 1988, The Genetics Society of Japan. All rights reserved.

    1988年, the japanese journal of genetics, 63 (6), 523 - 536

    研究論文(学術雑誌)

  • Takashige Ishii, Toru Terachi, Koichiro Tsunewaki

    Restriction endonuclease analysis of chloroplast (ct) DNA variation in two cultivated rice species, Oryza sativa and 0. glaberrima, using three endo-nucleases, EcoRI, Hindlll and PstI, indicated the differentiation of their chloroplast genome into three types. 0. sativa differed from 0. glaberrima in its EcoRI restriction pattern, whereas both 0. sativa ecospecies Japonica and ecosp. Javanica were differentiated from ecosp. Indica by their Hindlll and PstI restriction patterns. In contrast, no ctDNA variation was revealed in O. sativa ecosp. Japonica, Javanica, or Indica, or 0. glaberrima (11, 4, 7 and 8 accessions, respectively). These findings demonstrated, for the first time, the existence of chloroplast genome diversity among cultivated rice species and ecospecies. 1) Contribution from the Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan, No. 489. This work was supported in part by a Grant-in-Aid (No. 60400005) from the Ministry of Education, Science and Culture, Japan, and by a grant for the Joint-Research Program, the National Institute of Genetics, Misima, Japan. © 1986, The Genetics Society of Japan. All rights reserved.

    1986年, The Japanese Journal of Genetics, 61 (6), 537 - 541

    研究論文(学術雑誌)

MISC

  • Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species

    Naoki Mori, Yuko Hidehira, Takashige Ishii, Chiharu Nakamura

    The domestication of wheat and barley was the most important step in the emergence of farming communities that led to the ancient civilizations of Mesopotamia. Several lines of evidence indicate that emmer wheat was the earliest form of domesticated wheat derived from wild emmer wheat. Chloroplast DNA fingerprinting of wild and domesticated emmer wheat revealed that two dis

    2009年, Proc. 4th International Conference on Energy Efficiency and Agricultural Engineering, 725 - 729, 英語

  • The salinity tolerance evaluation of two eco-types of Oryza sativa L. at different growth stages in successive salinity levels.

    Javed M.A, ISHII Takashige, KAMIJIMA Osamu, MISOO Shuji

    2006年, 3rd. Int.. Conf. on Sustainable Agriculture for Food, Energy, and Industry, 英語

  • Photosynthesis, growth and yield of BC2F4 lines derived from Oryza sativa and wild rice species, O. rufipogon.

    Chisato Masumoto, Takashige Ishii, Tomoko Hatanaka, Naotsugu Uchida

    2004年, Proceedings of the 4th International Crop Science Congress, 26, 英語

  • QTL analysis for several agronomic characters using four BC2 poppulations between wild and cultivated rice species.

    ISHII Takashige

    2003年, Proc. Int. Genet.Resour. Work. Oryza., pp.7-10, 英語

  • Origins of domesticated emmer and common wheat inferred from chloroplast DNA fingerprinting.

    MORI Naoki, ISHII Takashige, ISHIDO T, HIROSAWA S, WATATANI H, KAWAHARA T, NESBITT M, BELAY G, TAKUMI Shigeo, OGIHARA Y

    2003年, Proc. Xth Int. Wheat Genet. Symp., Vol.1,pp.25-28, 英語

  • Variation in chloroplast microsatellite loci among wild and cultivated species of Emmer wheat

    Mori N, Ishii T, Ishido T, Belay G, Takumi S, Kawahara T, Ogihara Y, Nakamura C

    2002年, Proc 4th Int Triticeae Symp, 英語

  • ベトナム・ミヤンマーにおける野生イネの収集

    石井 尊生

    2000年, 近畿作物・育種研究, 45, 73 - 76, 日本語

  • RFLP分析に基づくイネ属Aゲノム種の系統関係

    石井尊生

    1994年, 東北大学遺伝生態研究センター通信, 26, 1 - 3

  • Aegilops seasii is a possible cytoplasmic donor to Ae. kotschyi and Ae. variabilis

    Siregar UJ, Ishii T, Tsunewaki K

    1988年, Proc 7th Int Wheat Genet Symp, 145 - 151

書籍等出版物

  • Rice Genomics, Genetics and Breeding - Domestication loci controlling panicle shape, seed shattering and seed awning

    Takashige Ishii, Ryo Ishikawa

    その他, Springer, 2018年, 英語

    学術書

  • 植物学の百科事典

    石井 尊生

    その他, 丸善出版(日本植物学会 編), 2016年06月, 日本語

    事典・辞書

  • 植物ゲノム科学辞典

    石井 尊生

    その他, 朝倉書店, 2009年04月, 日本語, 量的形質遺伝子座

    事典・辞書

  • ミヤンマーの野生イネ

    石井尊生

    アジア遊学 勉誠出版, 2003年

  • 葉緑体ゲノムからみた系統関係

    石井尊生, 常脇恒一郎

    稲学大成 農山漁村文化協会, 1990年

講演・口頭発表等

  • Target capture法に基づく日本と中国のウメの集団構造および系統関係について

    沼口孝司, 赤木剛士, 北村祐人, 石川亮, 石井尊生

    園芸学会 平成30年度秋季大会, 2019年03月, 日本語, 園芸学会, 明治大学, 国内会議

    口頭発表(一般)

  • QTL analysis for awnlessness using a backcross population between Oryza rufipogon and O. sativa IR36

    Priya J.A. Yaddehige, Rie Kuwata, Ryo Ishikawa, Takashige Ishii

    近畿作物・育種研究会 第186回例会, 2018年11月, 英語, 近畿作物・育種研究会, 京都大学, 国内会議

    口頭発表(一般)

  • 熱帯アジアにおける栽培イネから野生イネへの遺伝子流動について

    谷尾侑香, ORN Chhourn, 秋本正博, 宍戸理恵子, HTUN Than Myint, PHAN Phuong, Dang Thai, 野々村賢一, 小出陽平, 石川亮, 石井尊生

    育種学研究, 2018年09月22日, 日本語

  • 野生イネOryza meridionalisを用いて同定されたイネ種子亜鉛濃度を支配するqGZn9は連鎖した2つの遺伝子座によって制御される

    宮崎直哉, 谷古健太, 岩田将英, 門田剛太郎, Orn Chhourn, 吉田周作, 石井尊生, 馬建鋒, 石川亮

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    口頭発表(一般)

  • 穂の開帳性が栽培イネ(Oryza sativa)の穂形態に与える影響について

    窪田芳生, 西岡諒, 石井尊生, 石川亮

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    ポスター発表

  • ジャポニカ型栽培イネ(日本晴)の非脱粒性に関与する新規遺伝子座の離層形成への効果

    杉山昇平, 辻村雄紀, 大塚宏太郎, Than Myint Htun, 石井尊生, 石川亮

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    口頭発表(一般)

  • イネ育種における野生イネ利用の可能性

    石川亮, 宮崎直哉, 杉山昇平, 窪田芳生, 馬建鋒, 石井尊生

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    シンポジウム・ワークショップパネル(指名)

  • Exomeと果実・葉形状にみる日本のウメの多様性

    沼口孝司, 赤木剛士, 北村祐人, 大江孝明, 石川亮, 石井尊生

    日本育種学会第134回講演会, 2018年09月, 日本語, 日本育種学会, 岡山大学, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogon における種子脱粒性遺伝子座間の相互作用

    杉山昇平, Cristina Castillo, 岡佑美, Than Myint Htun, 野々村賢一, 石井尊生, 石川亮

    イネ遺伝学・分子生物学ワークショップ2018, 2018年07月, 日本語, 国立遺伝学研究所, 国内会議

    ポスター発表

  • オーストラリア由来の野生イネOryza meridionalis を用いたイネ種子亜鉛濃度を支配する遺伝子座の同定

    宮崎直哉, 谷古健太, 岩田将英, 門田剛太郎, Orn Chhourn, 吉田周作, 石井尊生, 馬建鋒, 石川亮

    イネ遺伝学・分子生物学ワークショップ2018, 2018年07月, 日本語, 国立遺伝学研究所, 国内会議

    ポスター発表

  • イネにおける小穂の開花メカニズムの解明に向けて

    小川風和, 石川亮, 石井尊生

    近畿作物・育種研究会 第185回例会, 2018年06月, 日本語, 近畿作物・育種研究会, 神戸大学, 国内会議

    口頭発表(一般)

  • ジャポニカ型栽培イネOryza sativa 日本晴の非脱粒性に関与する新規遺伝子座の同定

    辻村雄紀, Than Myint Htun, 沼口孝司, 高間菜摘, 杉山昇平, 石井尊生, 石川亮

    第59回日本植物生理学会年会, 2018年03月, 日本語, 植物生理学会, 札幌コンベンションセンター, 国内会議

    ポスター発表

  • 野生イネの繁殖に必須の種子脱粒性に関わる遺伝子座の同定

    辻村雄紀, Than Myint Htun, 沼口孝司, 高間菜摘, 杉山昇平, 石井尊生, 石川亮

    第49回種生物学シンポジウム, 2017年12月, 日本語, 種生物学会, すかっとランド九頭竜, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonと栽培イネOryza sativaの交雑系統群を利用した新規Na+ 輸送系遺伝子・変異の探索

    堀内智貴, 石川亮, 田野井慶太朗, 小林奈通子, 石井尊生, 堀江智明

    日本育種学会第132回講演会, 2017年10月, 日本語, 日本育種学会, 岩手大学, 国内会議

    口頭発表(一般)

  • ウメ遺伝資源における収穫期および果実形質の多様性

    沼口孝司, 北村祐人, 仲慶晃, 大江孝明, 土田靖久, 石川亮, 石井尊生

    日本育種学会第132回講演会, 2017年10月, 日本語, 日本育種学会, 岩手大学, 国内会議

    口頭発表(一般)

  • ウメ黒星病抵抗性に関与するQTLの同定に向けて

    沼口孝司, 北村祐人, 石川亮, 石井尊生

    園芸学会 平成29年度秋季大会, 2017年09月, 日本語, 園芸学会, 酪農学園大学, 国内会議

    口頭発表(一般)

  • 栽培イネと野生イネの戻し交雑系統群を用いたイネの花器官を支配する遺伝子座の同定

    石川 亮, 渡部 貴史, 西岡 諒, Pham Thien Thanh, 石井 尊生

    日本育種学会第131回講演会, 2017年07月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • インディカ型栽培イネIR36の非脱粒性を支配する新規遺伝子座の探索

    辻村 雄紀, 井上 千鶴, Than Myint Htun, 西村 彬則, 杉山 昇平, 岡 佑美, 石井 尊生, 石川 亮

    日本育種学会第131回講演会, 2017年07月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • 野生イネ(Oryza rufipogon)の遺伝的背景で芒を短くするためには - イネの栽培化における無芒性の検証にむけて –

    池本 麻衣, 大塚 光晴, 石川 亮, 石井 尊生

    日本育種学会第131回講演会, 2017年03月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    口頭発表(一般)

  • 栽培イネと野生イネの戻し交雑系統群を用いたイネの花器官を支配する遺伝子座の同定

    石川 亮, 渡部 貴史, 西岡 諒, Pham Thien Thanh, 石井 尊生

    日本育種学会第131回講演会, 2017年03月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • エンマーコムギの戻し交雑自殖系統を用いた栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Cristian, 石井 尊生, Kianian Shahryar, 森 直樹

    日本育種学会第131回講演会, 2017年03月, 日本語, 名古屋大学, [目的] エンマーコムギ (Triticum turgidum ssp. dicoccum) は、コムギ属の中で最も初期に栽培化されて起源した栽培種の一つであり、初期農耕の起源と世界各地への伝播において重要な役割を果たした。本研究では、コムギ栽培化の初期に起こった遺伝的変化を解明することを目的として、祖先野生種 (T. turgidum ssp. dicoccoides) の遺伝的背景を持つ戻し交雑自殖系統を育成し、栽培化関連形質を評価することによりこれらを支配するQTLの解析を進めている。 [方法] 栽培エンマーコムギ (以下Dcm1001と呼ぶ)と野生エンマーコムギ (以下Dcc63) の交雑から野生種の遺伝的背景を持つ戻し交雑自殖系統 (BC2F14) を育成した(図1)。これらの栽培化関連形質を評価し、分子マーカーによるQTL解析により、, 国内会議

    ポスター発表

  • インディカ型栽培イネIR36の非脱粒性を支配する新規遺伝子座の探索

    辻村 雄紀, 井上 千鶴, Than Myint Htun, 西村 彬則, 杉山 昇平, 岡 佑美, 石井 尊生, 石川 亮

    日本育種学会第131回講演会, 2017年03月, 日本語, 日本育種学会, 名古屋大学, 国内会議

    ポスター発表

  • 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Cristian, 石井 尊生, Kianian Shahryar, 森 直樹

    第11回ムギ類研究会, 2016年12月, 日本語, 岡山大学 資源植物学研究所, エンマーコムギはコムギ属の中で最も初期に栽培化されたコムギの一つとされている。本研究では、エンマーコムギの栽培化の初期に起こった遺伝的変化の解明を目的として、野生エンマーコムギと栽培エンマーコムギから作出された野生種の遺伝的背景を持つ戻し交雑自殖系統について、それらの栽培化関連形質を調査した。また、分子マーカーによるQTL解析を行うことで、エンマーコムギの栽培化関連形質を支配するQTLの染色体上の位置や効果を推定した。, 国内会議

    ポスター発表

  • 野生イネOryza rufipogon由来の穂の開帳性に関する遺伝子座と関連遺伝子の発現について

    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, 石川 亮, 石井 尊生

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑自殖系統を用いた節からの出芽能力の評価

    池本 麻衣, 大八木 徹弥, Pham Thien Thanh, 石川 亮, 石井 尊生

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonとインディカ型栽培イネ(カサラス)の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, 石井 尊生, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    口頭発表(一般)

  • ジャポニカ型栽培イネ(日本晴)の非脱粒性に関与した遺伝子座の推定

    辻村 雄紀, Than Myint Htun, 沼口 孝司, 石井 尊生, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • ウメ遺伝資源における黒星病およびかいよう病抵抗性の評価

    沼口 孝司, 北村 祐人, 土田 靖久, 仲 慶晃, 石川 亮, 石井 尊生

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    ポスター発表

  • Oryza rufipogonとインディカ型栽培イネ(カサラス)の戻し交雑集団を用いた種子脱粒性に関する新規遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, Than Myint Htun, 辻村 雄紀, 石井 尊生, 石川 亮

    日本育種学会第130回講演会, 2016年09月, 日本語, 日本育種学会, 鳥取大学, 国内会議

    口頭発表(一般)

  • Evaluation of seed-shattering behaviour in early rice domestication

    Ryo Ishikawa, Yumi Oka, Yuki Tsujimura, Natsumi Takama, Chizuru Inoue, Than Myint Htun, Takashige Ishii

    8th World Archaeology Congress, 2016年09月, 英語, Doshisha University, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • A closed panicle played an important role in rice domestication

    Ryo Nishioka, Koji Numaguchi, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii

    8th World Archaeology Congress, 2016年09月, 英語, Doshisha University, 国際会議

    ポスター発表

  • 野生イネOryza rufipogon由来の穂の開帳性を支配する遺伝子座の推定と効果の検証

    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, 石川 亮, 石井 尊生

    イネ遺伝学・分子生物学ワークショップ, 2016年07月, 日本語, 名古屋大学, 国内会議

    ポスター発表

  • 熱帯アジアの野生イネOryza rufipogon

    石井 尊生

    イネ属近縁野生種研究会, 2016年07月, 日本語, 遺伝学研究所, 国内会議

    口頭発表(一般)

  • インディカ型栽培イネ(カサラス)の非脱粒性を支配するsh4座以外の遺伝子座の探索

    岡 佑美, 竹中 祥尭, 高間 菜摘, 井上 千鶴, 辻村 雄紀, Than Myint Htun, 石井 尊生, 石川亮

    イネ遺伝学・分子生物学ワークショップ, 2016年07月, 日本語, 名古屋大学, 国内会議

    ポスター発表

  • 栽培イネの祖先となった野生イネとは?

    石井 尊生

    第6回北海道イネ研究会, 2016年06月, 日本語, 北海道大学, 国内会議

    口頭発表(一般)

  • Identification of quantitative trait loci controlling seed-shattering behaviour in rice

    Yuki Tsujimura, Than Myint Htun, Chizuru Inoue, Takashige Ishii, Ryo Ishikawa

    PAG Asia 2016, 2016年06月, 英語, Grand Copthorne Waterfront Hotel, 国際会議

    ポスター発表

  • Allelic interaction at awing loci in the genetic backgrounds of wild and cultivated rice

    Mai Ikemoto, Mitsuharu Otsuka, Pham Thien Thanh, Ryo Ishikawa, Takashige Ishii

    PAG Asia 2016, 2016年06月, 英語, Grand Copthorne Waterfront Hotel, 国際会議

    ポスター発表

  • 野生イネと栽培イネの間で検出されたアルミニウムストレス耐性を支配する遺伝子座qAl1の遺伝学的解析

    和田 もりえ, 岩田 将英, 横正 健剛, 吉田 周作, Pham Tien Thanh, 石井 尊生, 馬 建鋒, 石川 亮

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • 野生イネおよび栽培イネの遺伝的背景における芒の長さを支配する遺伝子座の対立遺伝子間の相互作用

    池本 麻衣, 大塚 光晴, 石川 亮, 石井 尊生

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑集団を用いた穂の開帳性に関するQTL解析

    西岡 諒, 三日月 裕美, 田中 裕, 沼口 孝司, 石川 亮, 石井 尊生

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • オーストラリア由来の野生イネOryza meridionalisを用いた種子亜鉛濃度に関わる遺伝子座の同定

    岩田 将英, 谷古 健太, 門田 剛太郎, Orn Chhourn, 吉田 周作, 石井 尊生, 馬 建鋒, 石川 亮

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • インディカ型栽培イネKasalathの脱粒性喪失に関与した新規遺伝子座の探索

    岡 佑美, 井上 千鶴, Than Myint Htun, 竹中 祥尭, 辻村 雄紀, 石井 尊生, 石川 亮

    日本育種学会第129回講演会, 2016年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    ポスター発表

  • 野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連形質の解析

    島田 沙織, 牛 恒一, Vladutu Christian, 石井 尊生, Kianian Shahryar, 森 直樹

    第10回 ムギ類研究会, 2015年12月, 日本語, 三重大学農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、野生エンマーコムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連形質の解析を行った。その結果、まだメーカー数が藤生部であるが穂軸の脆弱性や1穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, 国内会議

    ポスター発表

  • 栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を用いた栽培化関連QTLの解析

    宮崎 裕貴, Pham-Minh Ngoc, Vladutu Christian, 石井 尊生, Kianian Shahryar, 森 直樹

    第10回 ムギ類研究会, 2015年12月, 日本語, 三重大学 農学部, エンマーコムギの栽培化初期に起こった遺伝的変化を明らかにするため、栽培エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統(BC2F12)を育成し、SSRマーカーを用いた栽培化関連QTLの解析を行った。その結果、穂軸の脆弱性や1穂あたりの小穂数、粒数、など栽培化に関連した重要な形質に関与するQTLの座乗位置を推定することができた。, 国内会議

    ポスター発表

  • 野生二粒系コムギの遺伝的背景に栽培種の染色体断片を導入した戻し交雑自殖系統群の作出:栽培化関連形質の遺伝学的解析に向けて

    森 直樹, 牛 恒一, Vladutu Cristian, 石井 尊生, Kianian Shahryar

    日本育種学会第128回講演会, 2015年09月, 日本語, 新潟大学, エンマーコムギ(Triticum turgidum ssp. dicoccum)は今から約1万年前に野生二粒系コムギ(別名パレスチナコムギ、T. turgidum ssp. dicoccoides)から栽培化されて誕生した。このコムギは新石器時代から数千年にわたってムギ農耕の主要な作物として利用されたことが知られている。我々はこの栽培化の過程でゲノムにどのような変化が起こったのかを明らかにするため、これまでに栽培種と野生種の交雑に由来するF2集団や栽培種の遺伝的背景に野生種の染色体断片を導入した戻し交雑系統群を用いて解析してきた。今回、栽培化初期の遺伝的変化を捉えることを目的として、野生二粒系コムギの遺伝的背景に栽培エンマーコムギの染色体断片を導入した戻し交雑系統群を作成したので報告する。, 国内会議

    ポスター発表

  • 野生イネ(Oryza rufipogon) の遺伝的背景における種子脱粒性と穂の開帳性の関係

    井上 千鶴, Than Myint Htun, 石井 尊生, 石川 亮

    日本育種学会第128回講演会, 2015年09月, 日本語, 日本育種学会, 新潟大学, 国内会議

    ポスター発表

  • Evaluation of the domestication-related traits in rice

    Ryo Ishikawa, Yumi Oka, Ryo Nishioka, Mai Ikemoto, Than Myint Htun, Chizuru Inoue, Koji Numaguchi, Takashige Ishii

    Early rice cultivation systems and their impact on social evolution and the environment., 2015年09月, 英語, University College London, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Evaluation of genetic variation among wild and cultivated rice in Cambodia

    Orn Chhourn, Rieko Shishido, Masahiro Akimoto, Ryo Ishikawa, Than Myint Htun, Ryo Nishioka, Men Sarom, Sakhan Sophany, Ouk Makara, Takashige. Ishii

    日本育種学会第128回講演会, 2015年09月, 英語, 日本育種学会, 新潟大学, 国内会議

    ポスター発表

  • 野生イネOryza rufipogon由来の穂の開帳性を支配する遺伝子座の推定

    西岡 諒, 三日月 裕美, Than Myint Htun, 石川 亮, 石井 尊生

    近畿作物・育種研究会 第179回例会, 2015年05月, 日本語, 近畿作物・育種研究会, 奈良県立橿原考古学研究所, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogonと栽培イネO. sativaの戻し交雑自殖系統を用いた節からの出芽・出根能力およびひこばえの発生能力の評価

    池本 麻衣, 大八木 徹弥, Pham Thien Thanh, 石川 亮, 石井 尊生

    近畿作物・育種研究会 第179回例会, 2015年05月, 日本語, 近畿作物・育種研究会, 奈良県立橿原考古学研究所, 国内会議

    口頭発表(一般)

  • 野生イネと栽培イネのアルミニウムストレス耐性を支配する遺伝子座の同定とその効果

    和田 もりえ, 岩田 将英, 吉田 周作, Pham Thien Thanh, 石井 尊生, 馬 建鋒, 石川 亮

    日本育種学会第127回講演会, 2015年03月, 日本語, 日本育種学会, 玉川大学, 国内会議

    ポスター発表

  • インディカ型栽培イネ(Oryza sativa cv. IR36)における種子脱粒性の喪失に関与した遺伝子座数の推定

    西村 彬則, Than Myint Htun, 井上 千鶴, 岡 佑美, 石井 尊生, 石川 亮

    日本育種学会第127回講演会, 2015年03月, 日本語, 日本育種学会, 玉川大学, 国内会議

    ポスター発表

  • Evaluation of genetic variation among wild rice populations in Cambodia

    Orn Chhourn, Rieko Shishido, Hiroki Kageyama, Masahiro Akimoto, Ryo Ishikawa, Makara Ouk, Sarom Men, Takashige Ishii

    日本育種学会第127回講演会, 2015年03月, 英語, 日本育種学会, 玉川大学, 国内会議

    ポスター発表

  • A closed panicle regulated by OsLG1 was a selected trait during rice domestication

    TAKASHIGE ISHII

    Plant & Animal Genome XXIII, 2015年01月, 英語, San Diego, USA, 国際会議

    [招待有り]

    シンポジウム・ワークショップパネル(指名)

  • Seed shattering behaviour and abscission layer formation in rice

    Cizuru Inoue, Than Myint Htun, Yumi Oka, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa

    Marchantia Workshop 2014, 2014年12月, 英語, Centennial Hall, Kobe University, 国際会議

    ポスター発表

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座の相互作用と離層形態

    井上 千鶴, Than Myint Htun, 井上 加奈子, 池田 健一, 石井 尊生, 石川 亮

    日本育種学会第126回講演会, 2014年09月, 日本語, 日本育種学会, 南九州大学, 国内会議

    ポスター発表

  • 戻し交雑自殖系統を用いたエンマーコムギの栽培化関連形質の遺伝学的解析

    森 直樹, Ngoc Pham Minh, Vladutu Cristian, Thanh Pham Thien, Thanh Phan Thi, 石井 尊生, Kianian Shahryar

    日本育種学会 第126回講演会, 2014年09月, 日本語, 南九州大学, エンマーコムギ (Triticum turgidum ssp. dicoccum)は、約1万年前に野生種 (パレスチナコムギ、T. turgidum ssp. dicoccoides) から栽培化されて誕生した。このコムギは新石器時代から数千年にわたり西南アジアからヨーロッパにかけて主要な穀物として利用されたことが知られている。我々はコムギの栽培化初期の遺伝的変化を明らかにするため、エンマーコムギとパレスチナコムギのF1にエンマーコムギを戻し交雑し、エンマーコムギの遺伝的背景に野生種の染色体断片を導入した戻し交雑自殖系統を作成した。本講演では、この系統を用いて脱粒性などの栽培化関連形質のQTL解析を行ったので報告する。, 国内会議

    ポスター発表

  • 野生イネと栽培イネのアルミニウムストレス耐性を支配する遺伝子座の同定

    和田 もりえ, 岩田 将英, 吉田 周作, Pham Thien Thanh, 石井 尊生, 馬 建鋒, 石川 亮

    イネ遺伝学・分子生物学ワークショップ2014, 2014年07月, 日本語, 東京大学, 国内会議

    シンポジウム・ワークショップパネル(公募)

  • 野生イネ(Oryza rufipogon)における種子脱粒性遺伝子座間の相互作用

    井上 千鶴, Than Myint Htun, Orn Chhourn, 岡 佑美, 石井 尊生, 石川 亮

    イネ遺伝学・分子生物学ワークショップ2014, 2014年07月, 日本語, 東京大学, 国内会議

    シンポジウム・ワークショップパネル(公募)

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用

    井上 千鶴, Than Myint Htun, 石井 尊生, 石川 亮

    日本育種学会第125回講演会, 2014年03月, 日本語, 日本育種学会, 東北大学, 国内会議

    口頭発表(一般)

  • 野生イネ(Oryza rufipogon)の遺伝的背景における栽培化関連形質の評価

    西村 彬則, 岩崎 友哉, 山本 知佳, 石川 亮, 石井 尊生

    日本育種学会第125回講演会, 2014年03月, 日本語, 日本育種学会, 東北大学, 国内会議

    口頭発表(一般)

  • Identification of a quantitative trait locus involved in abscission layer formation for seed shattering in Asian wild rice, Oryza rufipogon

    Than Myint Htun, Chizuru Inoue, Orn Chhourn, Takashige Ishii, Ryo Ishikawa

    日本育種学会第125回講演会, 2014年03月, 英語, 日本育種学会, 東北大学, 国内会議

    口頭発表(一般)

  • Evaluation of genetic diversity among rice varieties in Cambodia

    Orn Chhourn, Ryo Ishikawa, Sakhan Sophany, Ouk Makara, Takashige Ishii

    7th International Conference of Rice Genetics, 2013年11月, 英語, Dusit Thai Hotel Manila, Philippine, 国際会議

    ポスター発表

  • Closed panicles in rice domestication

    Ishii T, Numaguchi K, Miura K, Yoshida K, Thanh PT, Htun TM, Yamasaki M, Matsumoto T, Terauchi R, Ishikawa R, Ashikari M

    International Rice Genetics Symposium, 2013年11月, 英語, Manila, Philippines, 国際会議

    口頭発表(一般)

  • A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L.

    Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, Takashige Ishii

    7th International Rice Genetics Symposium, 2013年11月, 英語, Dusit Thai Hotel Manila, Philippine, 国際会議

    ポスター発表

  • A closed panicle trait regulated by OsLG1 in domesticated rice, Oryza sativa L.

    Ryo Ishikawa, Kotaro Miura, Kentaro Yoshida, Koji Numaguchi, Than Myint Htun, Pham Thien Thanh, Takashi Matsumoto, Masanori Yamasaki, Ryohei Terauchi, Motoyuki Ashikari, Takashige Ishii

    7th International Conference of Rice Genetics, 2013年11月, 英語, Dusit Thai Hotel Manila, Philippine, 国際会議

    ポスター発表

  • 穂が閉じることによって野生イネはどう変化したか?

    沼口 孝司, Htun Than Myint, Thanh Pham Thien, 石川 亮, 石井 尊生

    日本育種学会第124回講演会, 2013年10月, 日本語, 日本育種学会, 鹿児島大学, 国内会議

    口頭発表(一般)

  • Evaluation of genetic variation among wild rice populations of Oryza rufipogon

    Ishii T

    Expert Consultation Workshop on the Use of Rice Wild Relatives for Rice Improvement, 2013年10月, 英語, Shizuoka, Japan, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Genetic analysis of domestication traits in emmer wheat using an F2 population

    Naoki Mori, Phan Thi Thanh, Cristian Vladutu, Pham Thien Thanh, Takashige Ishii, Miyuki Nitta, Shuhei Nasuda, Shahryar Kianian

    12th International Wheat Genetics Symposium,, 2013年09月, 英語, Pacifico Yokohama, Japan, Emmer wheat (Triticum turgidum ssp. dicoccum) was a principal crop in the development and spread of Neolithic agriculture in the Old World. It represents the primitive situation in the domestication of AABB tetraploid wheat. It has non free-threshing (hulled) habit and relatively fragile rachis, although it has non-shattering spike. The more advanced forms of tetraploid whea, 国際会議

    ポスター発表

  • Molecular genetic analysis of the QTLs involved in the domestication of emmer wheat

    Thanh P. T, C. Vladutu, S. Kianian, T. Ishii, T. Pham, S. Nasuda, M. Nitta, N. Mori

    日本育種学会 第122回講演会, 2012年09月, 英語, 京都産業大学, Significant association between wheat and human began in southwest Asia more than 10000 years ago. Emmer wheat was one of the founder crop that was domesticated from wild emmer wheat. In the present study, QTL analysis was carried out to estimate the valuable QTLs underlying the domestication of emmer wheat., 国内会議

    口頭発表(一般)

  • Evaluation of genetic variation among wild populations and local varieties of rice in Vietnam

    Ishii T

    Rice Symposium: Trends in Rice Research to Overcome Stresses in a context of Climate Change, 2012年09月, 英語, Hanoi, Vietnam, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Evaluation of genetic variation among wild populations and local varieties in rice

    Ishii T

    Rice and Language across Asia Symposium, 2011年11月, 英語, Cornell Univ, USA, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 山田錦の両親系統における酒造好適性にかかわる染色体領域の推定

    八軒 雄大, 吉田 晋弥, 石井 尊生, 太田 淳, 明石 貴裕, 西村 顕, 森 直樹

    日本育種学会 第120回講演会, 2011年09月, 日本語, 福井県立大学、福井, 国内会議

    ポスター発表

  • 野生イネの遺伝的背景における栽培イネの非脱粒性遺伝子の効果

    石川 亮, Than Myint Htun, 山崎 将紀, Pham Thien Thanh, 石井 尊生

    第52回日本植物生理学会年会, 2011年03月, 日本語, 日本植物生理学会, 東北大学, 国内会議

    口頭発表(一般)

  • 野生イネ (Oryza rufipogon)の種子有芒性の評価および遺伝子マッピング

    大塚 光晴, 石川 亮, 石井 尊生

    日本育種学会第119回講演会, 2011年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    口頭発表(一般)

  • 栽培イネ Oryza sativa Nipponbareを野生イネO. rufipogon W630で戻し交雑した自殖系統群を用いた植物と種子の形態形質に関するQTL解析

    山本 知佳, 岩崎 友哉, 石川 亮, 石井 尊生

    日本育種学会第119回講演会, 2011年03月, 日本語, 日本育種学会, 横浜市立大学, 国内会議

    口頭発表(一般)

  • Wild rice: Interesting materials for plant breeding and genetics

    Ishii T

    Special Seminar in International Platform for Asian Agricultural Education, 2010年11月, 英語, Kasetsart Univ, Thailand, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • Domestication of rice

    Ishii T

    Special Seminar in International Platform for Asian Agricultural Education, 2010年11月, 英語, Chiang Mai Univ, Thailand, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • Gene mapping using molecular markers

    Ishii T

    International Workshop on Agrobioresource Conservation and Pest Management in Southeast Asia, 2010年06月, 英語, Kobe, Japan, 国際会議

    公開講演,セミナー,チュートリアル,講習,講義等

  • QTL analysis for spikelet opening time using backcross inbred lines between Oryza sativa Nipponbare and O. rufipogon

    Pham Thien Thanh, Phan Dang, Thai Phuong, Ryo Ishikawa, Takashige Ishii

    日本育種学会第117回講演会, 2010年03月, 英語, 日本育種学会, 京都大学, 国内会議

    口頭発表(一般)

  • Estimation of outcrossing rate of Asian wild rice, Oryza rufipogon, under field condition

    Phan Dang Thai Phuong, Hiroki Kageyama, Ryo Ishikawa, Takashige Ishii

    日本育種学会第117回講演会, 2010年03月, 英語, 日本育種学会, 京都大学, 国内会議

    口頭発表(一般)

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

    Pham Thien Thanh, Ryo Ishikawa, Naoki Mori, Takashige Ishii

    6th International Conference of Rice Genetics, 2009年11月, 英語, Manila Hotel, Philippine, 国際会議

    ポスター発表

  • QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O. sativa Japonica Nipponbare

    Thanh PT, Ishikawa R, Mori N, Ishii T

    International Rice Genetics Symposium, 2009年11月, 英語, Manila, Philippines, 国際会議

    ポスター発表

  • Evaluation of genetic diversity among wild rice populations of Oryza rufipogon in Myanmar and Cambodia

    Ishii T

    International Symposium on Wild Rice, 2009年11月, 英語, Bangkok, Thailand, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Who was the mother of wheat?: a view from genetic variation in chloroplast DNA among wheat species

    Mori, N, Hidehira, Y, Ishii, T, Nakamura, C

    4th International Conference on Energy Efficiency and Agricultural Engineering, 2009年10月, 英語, Rousse, Bulgaria, 国際会議

    口頭発表(一般)

  • Intraspecific variation of chloroplast DNA in Aegilops speltoides

    Naoki Mori, Hiroyuki Watatani, Takashige Ishii, Yasutaka Kondo, Taihachi Kawahara, Chiharu Nakamura

    6th International Triticeae Symposium, 2009年06月, 英語, Kyoto University, Kyoto, Japan, 国際会議

    ポスター発表

  • Evaluation of genetic variation in rice

    Ishii T

    Asian International Symposium for Agriculture, 2009年03月, 英語, Kobe, Japan, 国際会議

    口頭発表(一般)

  • Mitochondrial microsatellite variability in the ancestral species of wheat (Aegilops speltoides Tausch.)

    Yoshiaki Ohmichi, Takashige Ishii, Chiharu Nakamura, Naoki Mori

    GENES & GENETIC SYSTEMS, 2007年12月, 英語, GENETICS SOC JAPAN

  • 野生イネ由来の種子脱粒性、穂の開帳性、種子色を支配する遺伝子のマッピング

    石井 尊生, 崎山 弘樹, 石川 亮, 上島 脩志

    近畿作物育種研究会, 2005年07月, 日本語, 近畿作物育種研究会, 神戸大学, 国内会議

    口頭発表(一般)

  • 野生イネ由来の種子脱粒性, 穂の開帳性, 種子色を支配する遺伝子のマッピング

    石井 尊生, 崎山 弘樹, 石川 亮, 上島 脩志

    近畿作物・育種研究会第159回例会, 2005年, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 野生イネ遺伝資源のオンファーム保存システム構築のための実証的調査Ⅰ ミャンマーにおける事例

    秋本 正博, 宍戸 理恵子, 石井 尊生, 石川 隆二, 上埜 喜八, 中村 郁郎, 佐藤 洋一郎, 佐藤 雅志

    日本育種学会(育種学研究第7卷別冊1-2号p.296), 2005年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogon由来の光合成能力関連QTLをもつ準同質遺伝子系統の光合成特性

    増本 千都, 石井 尊生, 池田 延行, 畠中 知子, 内田 直次

    日本作物学会(日本作物学会紀事74巻第1号96-97), 2005年, 日本語, 日本作物学会, 未記入, 国内会議

    ポスター発表

  • 普通糸コムギならびにその祖先種でみられたコムギミトコンドリアマイクロサテライト座における多様性

    高橋 知佐, 森 直樹, 上島 脩志, 石井 尊生

    近畿作物・育種研究会第159回例会, 2005年, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 耐塩性および非耐塩性イネ間のFl雑種の葯培養における培地中NaCl濃度の影響

    三十尾 修司, 松本 学, Javed, M. A, 石井 尊生, 上島 脩志

    日本育種学会(育種学研究第7卷別冊1-2号p.236), 2005年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • ミャンマーにおける野生イネの調査

    石井 尊生

    日本育種学会(育種学研究第7卷別冊1-2号p.80), 2005年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • The salinity tolerance evaluation of two eco-types of Oryza sativa L. at different growth stages in successive salinity levels.

    Javed M. A, ISHII Takashige, KAMIJIMA Osamu, MISOO Shuji

    3rd Int. Conf. on Sustainable Agriculture for Food, Energy, and Industry,, 2005年, 英語, 未記入, Canada, 国際会議

    口頭発表(一般)

  • QTL analysis for several agronomic characters using BC2 populations between Oryza meridionalis and O. sativa.

    ISHII Takashige, ITO K, HARA D, KOBAYASHI N, UCHIDA Naotsugu, KAMIJIMA Osamu

    5th Int. Rice Genet. Symp,, 2005年, 英語, 未記入, Philippines, 国際会議

    口頭発表(一般)

  • Production and evaluation of chromosome segment substitution lines having wild QTL regions for yield, photosynthesis activity and grain width between Oryza sativa cv. IR36 and O. rufipogon.

    IKEDA N, ASHIDA T, MASUMOTO C, UCHIDA Naotsugu, KAMIJIMA Osamu, ISHII Takashige

    5th Int. Rice Genet. Symp,, 2005年, 英語, 未記入, Philippines, 国際会議

    口頭発表(一般)

  • Improvement of the anther culture efficiency in recalcitrant salt tolerant indica rice varieties.

    Javed M. A, ISHII Takashige, KAMIJIMA Osamu, MIOO Shuji

    The 1st. Int.. Symp. on Sustainable Agriculture for Subtropical Regions, 67,, 2005年, 英語, 未記入, China, 国際会議

    口頭発表(一般)

  • 栽培イネ(Oryza sativa Nipponbare)の遺伝背景における野生イネ(Oryza rufipogon)由来の脱粒性遺伝子の作用について

    二村 直人, 石川 亮, 上島 脩志, 石井 尊生

    日本育種学会第106回講演会, 2004年09月, 日本語, 日本育種学会, 三重大学, 国内会議

    口頭発表(一般)

  • イネ品種「兵庫北錦」と「北陸142号」の雑種集団を用いた心白発現及び粒大に関するQTL分析

    澤田 桂子, 久世 淳子, 吉田 晋也, 池上 勝, 中村 千春, 石井 尊生, 上島 脩志

    日本育種学会第106回講演会, 2004年09月, 日本語, 日本育種学会, 三重大学, 国内会議

    口頭発表(一般)

  • 野生イネOryza. rufipogon および栽培イネO. sativa Nipponbare交配由来のBC2F4集団を用いた諸形質に関するQTL解析

    山下 晃弘, 内田 直次, 上島 脩志, 石井 尊生

    日本育種学会, 2004年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 栽培イネ(Oryza sativa Nipponbare) の遺伝的背景における野生イネ(O. rufipogon)由来の脱粒性遺伝子の作用について

    二村 直人, 石川 亮, 上島 脩志, 石井 尊生

    日本育種学会, 2004年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • イネ品種「兵庫北錦」と「北陸142号」の雑種集団を用いた心白発現および粒大に関するQTL解析

    澤田 桂子, 久世 淳子, 吉田 晋弥, 池上 勝, 中村 千春, 石井 尊生, 上島 脩志

    日本育種学会, 2004年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • Estimation and comparison of salinity tolerance during seedling stage in several indica rice cultivars.

    JAVED M A, MISOO Shuji, ISHII Takashige, KAMIJIMA Osamu

    日本育種学会, 2004年, 英語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogon由来の脱粒性遺伝子のマッピング

    二村 直人, 石川 亮, 上島 脩志, 石井 尊生

    日本育種学会第104回講演会, 2003年09月, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 祖先2倍種の葉緑体DNA における分子多型から見た4倍性コムギの起原

    綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹

    日本育種学会 育種学研究, 2003年09月, 日本語, 日本育種学会, 神戸大学, 国内会議

    口頭発表(一般)

  • 野生イネ(Oryza rufipogon)の二つのアルコール脱水素酵素遺伝子座領域(Adh1 とAdh2)における塩基多型

    吉田 健太郎, 石井 尊生, 宮下 直彦

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 野生イネでみられた葉緑体およびミトコンドリアマイクロサテライト座における多型性

    隈本 寿彦, 佐藤 雅志, 石川 隆二, 佐藤 洋一郎, 上島 脩志, 石井 尊生

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 野生イネOryza rufipogon 由来の脱粒性遺伝子のマッピング

    二村 直人, 石川 亮, 上島 脩志, 石井 尊生

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 米アミロペクチンの超長鎖合成の遺伝解析

    青木 法明, 梅本 貴之, 石井 尊生, 吉田 晋弥, 上島 脩志, 松倉 潮, 井ノ内 直良

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 窒素固定バクテリアエンドファイトHerbaspirillum sp.B501 の内生菌数にみられる野生および栽培イネ間差異

    安部 匡, 南沢 究, 石井 尊生, 東谷 篤志, 佐藤 雅志

    神戸大学農学部秋季公開セミナー-農学を新発見-., 2003年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • 祖先2倍種の葉緑体DNA における分子多型からみた4倍性コムギの起原

    綿谷 浩之, 石井 尊生, 河原 太八, 山根 京子, 中村 千春, 森 直樹

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • 栽培イネOryza sativa × 野生イネO. rufipogon のBC2F2 系統の光合成特性− Rubisco, Rubiscoactivase, スクロース代謝関連酵素に関して−

    増本 千都, 石井 尊生, 内田 直次

    日作紀, 2003年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • イネ栽培種×野生種交雑系統BC2F4 系統の光合成と生長

    増本 千都, 石井 尊生, 内田 直次

    熱帯農業, 2003年, 日本語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • アズキにおけるマイクロサテライトマーカーの開発

    松尾 明子, 渡辺 純平, 池田 優子, 伊勢村 武久, 石井 尊生, 上島 脩志

    日本育種学会,育種学研究, 2003年, 日本語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • QTL analysis for several agronomic characters using four BC2 poppulations between wild and cultivated rice species.

    ISHII Takashige

    Int. Genet. Resour. Workshop on the genus Oryza., 2003年, 英語, 未記入, 未記入, 国内会議

    口頭発表(一般)

  • Effects of culture media and alternate temperatures on anther culture of salt tolerant indica rice cultivars.

    JAVED M A, MISOO Shuji, ISHII Takashige, KAMIJIMA Osamu

    日本育種学会,育種学研究, 2003年, 英語, 日本育種学会, 未記入, 国内会議

    口頭発表(一般)

  • イネ野生種(Oryza rufipogon)由来の脱粒性遺伝子のマッピング

    石川 亮, 上島 脩志, 石井 尊生

    日本育種学会第101回講演会, 2002年03月, 日本語, 日本育種学会, 玉川大学, 国内会議

    口頭発表(一般)

  • タケアズキ×アズキの雑種集団を用いた連鎖地図の作成

    加賀秋人, 月本恭幸, 所英理子, 石井尊生, 木村忠彦, 上島おさ志

    育種学雑誌, 1996年, 日本語

  • アズキ×ヒメツルアズキの雑種分離集団における諸形質とRAPDマーカーの遺伝分析

    加賀秋人, 大西昌子, 石井尊生, 三十尾修司, 木村忠彦, 上島おさ志

    育種学雑誌, 1994年, 日本語

所属学協会

  • 近畿作物育種研究会

  • 日本遺伝学会

  • 日本育種学会

共同研究・競争的資金等の研究課題

  • 石井 尊生

    科学研究費補助金/基盤研究(B), 2018年04月 - 2022年03月, 研究代表者

    競争的資金

  • 石井 尊生

    学術研究助成基金助成金/挑戦的研究(萌芽), 2018年06月 - 2021年03月, 研究代表者

    競争的資金

  • 石井 尊生

    特別研究員奨励費, 2016年04月 - 2018年03月, 研究代表者

    競争的資金

  • 石井 尊生

    科学研究費補助金/基盤研究(B), 2014年04月 - 2018年03月, 研究代表者

    競争的資金

  • 石井 尊生

    科学研究費補助金/基盤研究(B), 2014年04月 - 2017年03月, 研究代表者

    競争的資金

  • 石井 尊生

    科学研究費補助金/基盤研究(C), 2008年04月 - 2011年03月, 研究代表者

    競争的資金

  • 石井 尊生

    特別研究員奨励費, 2011年, 研究代表者

    競争的資金

  • 石井 尊生

    学術研究助成基金助成金/基盤研究(C), 2011年, 研究代表者

    競争的資金

  • 石井 尊生

    科学研究費補助金/基盤研究(B), 2006年04月 - 2009年03月, 研究代表者

    競争的資金

  • 石井尊生

    日本学術振興会, 日仏交流促進事業(SAKURAプログラム), 神戸大学, 2006年09月 - 2008年08月, 研究代表者, 国際共著している

    競争的資金

  • 野生イネに潜む有用形質を支配する遺伝子の検出―野生種を利用した稲育種に向けて―

    石井 尊生

    日本学術振興会, 科学研究費助成事業 奨励研究(A), 奨励研究(A), 神戸大学, 2000年 - 2001年

    イネ野生種が持つ栽培種に存在しない有用遺伝子を検出するため、以下の実験を行った。まず、野生イネの材料としてミヤンマー由来のOryza rufipogon W630を用い、2種類の典型的な栽培品種(O. sativa Japonica cv. NipponbareおよびO. sativa Indica cv. IR36)で交雑し、それぞれ約200個体からなるBC_2戻し交雑集団を作成した。次に、これらを圃場に展開し、出穂期、最大光合成能、桿長、穂長、有効分げつ数、、種子100粒重、籾長、籾幅、玄米長、玄米幅、収量の11形質について調査した。BC_2集団は様々な形質を調査し終わる登熟期まで、葉を大量にサンプリングできなかったので、各BC_2自植後代約15系統の幼葉をまとめて回収しDNAを抽出した。これらのDNAは鋳型として調整し、マイクロサテライトマーカー座についての遺伝子型を調べるためにPCRを行った。なお、使用したマイクロサテライトマーカーは交配親間で多型がみられ、ほぼゲノムをカバーしている約75マーカーであった。11形質についてのデータならびに分子マーカー座の遺伝子型のデータをもとに、量的形質遺伝子座(QTL)解析を行ったところ、有意となった領域はNipponbare戻し交雑集団では55ヶ所、IR36戻し交雑集団では51ヶ所見出された。これらのうち、それぞれ半数強の領域において、野生種の対立遺伝子が各形質の計測値を増加させる効果を持っていた。2つの異なった栽培品種の遺伝的背景においては、野生種由来のQTLは6領域で共通していた。収量に関しては、第1染色体上の1領域で検出された野生種のQTLが両集団において収量を向上させる効果を持っていた。従って、この野生種由来のQTLは栽培イネのJaponicaおよびlndica双方において収量を向上させる効果を持つ可能性が示唆された。

  • 種およびゲノム特異的突然変異に基づくイネ属植物の系統進化の解明

    石井 尊生

    日本学術振興会, 科学研究費助成事業 奨励研究(A), 奨励研究(A), 神戸大学, 1997年 - 1998年

    分子レベルでの変異に基づくイネ属植物の系統進化の解明のため、以下の実験を行った。材料はAゲノム種より栽培種2種29系統、野生種2種30系統、Aゲノム以外の種より野生種6種20系統(Cゲノム種3系統、CDゲノム種5系統、BCゲノム種5系統、Eゲノム種4系統、Fゲノム種1系統、未同定4倍体ゲノム種1系統)の計79系統を用いた。これらよりDNAを抽出し、まず葉緑体ゲノムにおける遺伝子間領域20カ所をPCR法により増幅した。Aゲノム種内では増幅断片長にほとんど変異がみられなかったが、イネ属植物内では約半数の領域で欠失により生じた多型がみられた。次に、核・葉緑体の両ゲノムの単純反復配列領域についてもPCR法により増幅を行った。核ゲノム由来の24領域と葉緑体ゲノム由来の10領域において検出された増幅断片長の多型の割合を比較したところ、核ゲノムにおける単純反復配列領域は非常に増幅突然変異に富むことが観察された。以上の結果より、イネ属植物における系統関係について以下のことが明らかになった。イネ属植物内では、E、Fゲノム種が他のものと比較して大きく分化しており、A、BC、C、CDゲノム種はそれぞれ同程度に分化しクラスターを形成している。Aゲノム種内においては、野生種のO.perennisのオセアニア、アフリカおよびアメリカ型が大きく分化しており、栽培種のO.sativaはO.perennisのアジア型と、もう1つの栽培種であるO.glaberrimaは野生種のO.breviligulataとグループを作ることが明らかになった。これは、栽培種のO.sativaとO.glaberrimaの祖先野生種はそれぞれO.perennisのアジア型とO.breviligulataであることを示唆するものであった。

  • RFLPおよびRAPD分析法による栽培イネの祖先の解明

    石井 尊生

    日本学術振興会, 科学研究費助成事業 奨励研究(A), 奨励研究(A), 神戸大学, 1995年 - 1995年

    本研究では分子生物的手法を用いて栽培イネの祖先を明らかにすることを試みた。材料は栽培イネを含むAゲノム種より、栽培種2種14系統(O. sativa8系統、O. glaberrima6系統)、栽培イネと同じゲノム構成を持つ野生種2種16系統(O. perennis13系統、O. breviligulata3系統)の、計30系統を用いた、これらよりDNAを抽出し、分子レベルでの変異を検出するため、核、葉緑体、ミトコンドリアの3つのゲノムについてのRFLP分析および全DNAを用いたRAPD分析を行った。さらに、これら4つの分析それぞれについて、各系統間における類緑度を求め系統樹を作成した。まず、核、葉緑体、ミトコンドリアの3つのゲノム間における分化の違いについては、これらはほぼ同調して分化していたが、特に核ゲノムでの分化の程度が他の2つより大きいことが明らかになった。さらに、RFLP分析とRAPD分析の変異の検出頻度について比較したところ、RAPD分析の方がより多くの変異を検出できることがわかった。以上の結果をまとめ、総合的に分子レベルにおける栽培イネと野生イネとの系統関係について調べたところ、Aゲノム種内において、野生種のO. perennisのオセアニア、アフリカおよびアメリカ型が大きく分化しており、栽培種のO. sativaはO. perennisのアジア型と、もう1つの栽培種であるO. glaberrimaは野生種のO. brebiligulataとグループを作ることが明らかになった。これは、栽培種のO. sativaとO. glaberrimaの祖先野生種はそれぞれO. perennisのアジア型とO. breviligulataであることを示唆するものであった。