研究者紹介システム
| 杉本 幸裕 |  |
| スギモト ユキヒロ | |
| 大学院農学研究科 生命機能科学専攻 | |
| 教授 | |
| 農芸化学関係 |
研究者情報
所属
【主配置】
大学院農学研究科 生命機能科学専攻【配置】
農学部 生命機能科学科
学位
研究シーズ
研究シーズ紹介サイトへのリンク授業科目
- 特定課題演習 I-1, 大学院農学研究科, 2021
- 課題開発演習, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅰ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-1, 大学院農学研究科, 2021
- 植物機能化学, 大学院農学研究科, 2021
- 特定課題演習 I-2, 大学院農学研究科, 2021
- 生命機能科学プレゼンテーション演習Ⅱ（オーラル・ペーパー）, 大学院農学研究科, 2021
- 特定課題演習 II-2, 大学院農学研究科, 2021
- 生理活性物質論, 大学院農学研究科, 2021
- 卒業研究, 農学部, 2021
- 応用生命化学基礎英語２, 農学部, 2021
- 高度教養セミナー農学部応用生命化学入門, 農学部, 2021
- 高度教養セミナー農学部応用生命化学入門, 農学部, 2021
- 高度教養セミナー農学部応用生命化学, 農学部, 2021
- 高度教養セミナー農学部応用生命化学, 農学部, 2021
- 植物機能化学, 農学部, 2021
- 植物機能化学１, 農学部, 2021
- 植物天然物化学１, 農学部, 2021
- 生命機能科学各論, 農学部, 2021
- 生命機能科学各論II, 農学部, 2021
- 植物天然物化学, 農学部, 2021
- 植物機能化学２, 農学部, 2021
- 植物天然物化学２, 農学部, 2021
ジャンル
コメントテーマ
研究ニュース
研究活動
研究キーワード
研究分野
委員歴
- 2004年 - 現在, 植物化学調節学会, 評議員・代議員
- 2016年 - 2020年, 日本農芸化学会, 代議員
- 2008年 - 2010年, 日本農芸化学会, 代議員
- 2006年 - 2008年, 植物細胞分子生物学会, 評議員
受賞
2011年05月 日本農芸化学会, B.B.B.論文賞, Vestitol as a Chemical Barrier against Intrusion of Parasitic Plant Striga hermonthica into Lotus japonicus Roots
国内学会・会議・シンポジウム等の賞
2008年10月 植物化学調節学会, 植物化学調節学会賞, 「根寄生植物と宿主植物の相互作用に関する生物有機化学的研究」
国内学会・会議・シンポジウム等の賞
論文
The potato cyst nematode (PCN) causes extensive crop losses worldwide. Because the hatching of PCN requires host-derived molecules known as hatching factors (HFs), regulating HF production in host plants may help to control this harmful pest. Solanoeclepin A (SEA), isolated from potato, is the most active HF for PCN; however, its biosynthesis is completely unknown. We discovered a HF called solanoeclepin B (SEB) from potato and tomato root exudates and showed that SEB was biosynthesized in the plant and converted to SEA outside the plant by biotic agents. Moreover, we identified five SEB biosynthetic genes encoding three 2-oxoglutarate-dependent dioxygenases and two cytochrome P450 monooxygenases in tomato. Exudates from tomato hairy roots in which each of the genes was disrupted contained no SEB and had low hatch-stimulating activity for PCN. These findings will help to breed crops with a lower risk of PCN infection.
American Association for the Advancement of Science (AAAS), 2023年03月17日, Science Advances, 9 (11)研究論文（学術雑誌）
In sub-Saharan Africa, upland rice cultivation is expanding into rainfed areas endemic to the root parasitic weed Striga hermonthica. We evaluated the Striga resistance of 69 accessions from the World Rice Core Collection (WRC) to estimate the phenotypic diversity within the Oryza sativa species. Pre-attachment resistance was screened based on the germination-inducing activities of the root exudates, while post-attachment resistance was screened through rhizotron evaluation. The 69 WRC accessions showed a wide variation in both pre- and post-attachment resistance. Root exudates of one accession induced 0.04% germination, and those of some accessions displayed >80% germination. In the evaluation of post-attachment resistance, the successful parasitism percentages ranged from 1.3% to 60.7%. The results of these resistance evaluations were subjected to cluster analysis, which recognized five groups: group I of 27 accessions, with high pre- and post-attachment resistance; group II of 12 accessions, with high post-attachment resistance but moderate pre-attachment resistance; group III of 4 accessions, with low pre-attachment resistance; group IV of 13 accessions, with low post-attachment resistance; and group V of 13 accessions, with low pre- and post-attachment resistance. The wide variation found in the WRC accessions will help to elucidate the genetic factors underpinning pre- and post-attachment resistance.
MDPI AG, 2022年12月20日, Plants, 12 (1), 19 - 19研究論文（学術雑誌）
Canonical strigolactones (SLs), such as orobanchol, consist of a tricyclic lactone ring (ABC-ring) connected to a methylbutenolide (D-ring). Tomato plants have been reported to produce not only orobanchol but also various canonical SLs related to the orobanchol structure, including orobanchyl acetate, 7-hydroxyorobanchol isomers, 7-oxoorobanchol, and solanacol. In addition to these, structurally unidentified SL-like compounds known as didehydroorobanchol isomers (DDHs), whose molecular mass is 2 Da smaller than that of orobanchol, have been found. Although the SL biosynthetic pathway in tomato is partially characterized, structural elucidation of DDHs is required for a better understanding of the entire biosynthetic pathway. In this study, three novel canonical SLs with the same molecular mass as DDHs were identified in tomato root exudates. The first was 6,7-didehydroorobanchol, while the other two were not in the DDH category. These two SLs were designated phelipanchol and epiphelipanchol because they induced the germination of Phelipanche ramosa, a noxious root parasitic weed of tomato. We also proposed a putative biosynthetic pathway incorporating these novel SLs from orobanchol to solanacol.
Frontiers Media SA, 2022年12月14日, Frontiers in Plant Science, 13研究論文（学術雑誌）
Abstract Strigolactones (SLs) known as rhizosphere signaling molecules and plant hormones regulating shoot architecture, are classified into two distinct groups, canonical and non-canonical SLs based on their structures. Avenaol, a non-canonical SL found in the root exudates of black oat (Avena strigosa), has a characteristic bicyclo[4.1.0]heptane skeleton. Elucidating the biosynthetic mechanism of this peculiar structure is a challenge for further understanding the structural diversification of non-canonical SLs. In this study, a novel non-canonical SL, 6-epi-heliolactone in black oat root exudates was identified. Feeding experiments showed that 6-epi-heliolactone was a biosynthetic intermediate between methyl carlactonoate and avenaol. Inhibitor experiments proposed the involvement of 2-oxoglutarate-dependent dioxygenase in converting 6-epi-heliolactone to avenaol. These results provide new insights into the stereochemistry diversity of non-canonical SLs and a basis to explore the biosynthetic pathway causing avenaol.
Informa UK Limited, 2022年05月13日, Bioscience, Biotechnology, and Biochemistry研究論文（学術雑誌）
Abstract Root parasitic weeds of the Orobanchaceae, such as witchweeds (Striga spp.) and broomrapes (Orobanche and Phelipanche spp.), cause serious losses in agriculture worldwide, and efforts have been made to control these parasitic weeds. Understanding the characteristic physiological processes in the life cycle of root parasitic weeds is particularly important to identify specific targets for growth modulators. In our previous study, planteose metabolism was revealed to be activated soon after the perception of strigolactones in germinating seeds of O. minor. Nojirimycin inhibited planteose metabolism and impeded seed germination of O. minor, indicating a possible target for root parasitic weed control. In the present study, we investigated the distribution of planteose in dry seeds of O. minor by matrix-assisted laser desorption/ionization–mass spectrometry imaging. Planteose was detected in tissues surrounding—but not within—the embryo, supporting its suggested role as a storage carbohydrate. Biochemical assays and molecular characterization of an α-galactosidase family member, OmAGAL2, indicated that the enzyme is involved in planteose hydrolysis in the apoplast around the embryo after the perception of strigolactones, to provide the embryo with essential hexoses for germination. These results indicate that OmAGAL2 is a potential molecular target for root parasitic weed control.
Oxford University Press (OUP), 2022年04月05日, Journal of Experimental Botany, 73 (7), 1992 - 2004, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- 2022年04月, Natural Product Research., 36 (9), 2215 - 2222, 英語Conversion of methyl carlactonoate to heliolactone in sunflower
[査読有り]
研究論文（学術雑誌）
Strigolactones (SLs), a class of phytohormones that regulate diverse developmental processes, were initially characterized as host-derived germination stimulants for seeds belonging to the genera Striga, Orobanche, and Phelipanche. Orobanchol (1), which is detected in the root exudates of several plants and recognized as a prevalent SL, was first isolated from the root exudates of red clover as a germination stimulant for Orobanche minor in 1998. However, the structure of this stimulant proposed at that time was disputable considering its predicted germination-inducing activity for Striga gesnerioides. The genuine structure of orobanchol was elucidated following a decade-long controversy, which ultimately facilitated the understanding of the importance of SL stereochemistry in Striga seed germination. Recently, studies focusing on clarifying the biosynthesis pathway of orobanchol are being conducted. Cytochrome P450 monooxygenases are involved in orobanchol biosynthesis downstream of carlactonoic acid (CLA) via two pathways: either through 4-deoxyorobanchol or direct conversion from CLA. Substantial progress in the identification of more SL structures and clarification of their biosynthetic mechanisms will further contribute in the comprehension of their structural diversity’s functional importance and agricultural applications. Herein, we have reviewed the history leading to the discovery of the genuine structure of orobanchol and the current understanding of its biosynthetic mechanisms.
Frontiers Media SA, 2022年02月09日, Frontiers in Plant Science, 13, 国際誌[査読有り]
研究論文（学術雑誌）
- 2022年, Plant Cell PhysiolTandem Gene Duplication of Dioxygenases Drives the Structural Diversity of Steroidal Glycoalkaloids in the Tomato Clade,
[査読有り]
- Springer Science and Business Media LLC, 2021年11月, Planta, 254 (5)
[査読有り]
研究論文（学術雑誌）
- Elsevier BV, 2021年10月, Tetrahedron Letters, 85, 153469 - 153469
[査読有り]
研究論文（学術雑誌）
Tomato (Solanum lycopersicum) contains α-tomatine, a steroidal glycoalkaloid (SGA) that contributes to its defense against pathogens and herbivores through its bitter taste and toxicity. It accumulates at high levels in all the plant tissues, especially in leaves and immature green fruits, whereas it decreases during fruit ripening through metabolic conversion to the nontoxic esculeoside A, which accumulates in the mature red fruit. This study aimed to identify the gene encoding a C-27 hydroxylase that is a key enzyme in the metabolic conversion of α-tomatine to esculeoside A. The E8 gene, encoding a 2-oxoglutalate-dependent dioxygenase, is well-known as an inducible gene in response to ethylene during fruit ripening. The recombinant E8 was found to catalyze the C-27 hydroxylation of lycoperoside C to produce prosapogenin A and is designated as Sl27DOX. The ripe fruit of E8/Sl27DOX-silenced transgenic tomato plants accumulated lycoperoside C and exhibited decreased esculeoside A levels compared with the wild-type plants. Furthermore, E8/Sl27DOX-deletion in tomato accessions resulted in higher lycoperoside C levels in ripe fruit than in wild-type plants. Thus, E8/Sl27DOX functions as a C-27 hydroxylase of lycoperoside C in the metabolic detoxification of α-tomatine during tomato fruit ripening, and the efficient detoxification by E8/27DOX may provide an advantage in the domestication of cultivated tomatoes.
2021年10月, Plant & cell physiology, 65 (5), 775 - 786, 英語, 国内誌[査読有り]
研究論文（学術雑誌）
- Pesticide Science Society of Japan, 2021年08月20日, Japanese Journal of Pesticide Science, 46 (2), 136 - 142
研究論文（学術雑誌）
- Wiley, 2021年08月09日, The Plant Journal, 108 (1), 81 - 92
[査読有り]
研究論文（学術雑誌）
- Pesticide Science Society of Japan, 2021年05月20日, Journal of Pesticide Science, 46 (2), 242 - 247
[査読有り]
研究論文（学術雑誌）
- 2021年02月, Nature Communications, 英語The biosynthetic pathway of potato solanidanes diverged from that of spirosolanes due to evolution of a dioxygenase
[査読有り]
研究論文（学術雑誌）
- Springer US, 2021年, Methods in Molecular Biology, 13 - 23
論文集(書籍)内論文
- Elsevier BV, 2021年, Tetrahedron Letters, 68, 152922 - 152922, 英語
[査読有り]
研究論文（学術雑誌）
- 2021年, Plant PhysiolIdentification and characterization of sorgomol synthase in sorghum strigolactone biosynthesis;
[査読有り]
Orobanchaceae root parasitic weeds cause serious agricultural damage worldwide. Although numerous studies have been conducted to establish an effective control strategy for the growth and spread of root parasitic weeds, no practical method has been developed so far. Previously, metabolomic analyses were conducted on germinating seeds of a broomrape, Orobanche minor, to find novel targets for its selective control. Interestingly, planteose metabolism was identified as a possible target, and nojirimycin (NJ) selectively inhibited the germination of O. minor by intercepting planteose metabolism, although its precise mode of action was unclear. Here, transcriptome analysis by RNA-Seq was conducted to obtain molecular insight into the effects of NJ on germinating O. minor seeds. Differential gene expression analysis results suggest that NJ alters sugar metabolism and/or signaling, which is required to promote seed germination. This finding will contribute to understanding the effect of NJ and establishing a novel strategy for parasitic weed control.
2020年12月, J Pest Sci;, 45 (4), 230 - 237[査読有り]
研究論文（学術雑誌）
- Japanese Society for Plant Cell and Molecular Biology, 2020年09月, Plant Biotechnology, 37 (3), 319 - 325
[査読有り]
研究論文（学術雑誌）
Heliolactone is one of the earliest identified non-canonical strigolactones. Its concise synthesis was achieved by employing Knoevenagel-type condensation and semi-reduction of a malonate intermediate as the key steps. This synthesis was performed in a non-stereoselective manner, and thus a racemic and diastereomeric mixture of heliolactone was obtained. The developed synthetic route is fairly concise and straightforward.
Informa UK Limited, 2020年06月02日, Bioscience, Biotechnology, and Biochemistry, 84 (6), 1113 - 1118, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
MAIN CONCLUSION: CYP722C from cotton, a homolog of the enzyme involved in orobanchol synthesis in cowpea and tomato, catalyzes the conversion of carlactonoic acid to 5-deoxystrigol. Strigolactones (SLs) are important phytohormones with roles in the regulation of plant growth and development. These compounds also function as signaling molecules in the rhizosphere by interacting with beneficial arbuscular mycorrhizal fungi and harmful root parasitic plants. Canonical SLs, such as 5-deoxystrigol (5DS), consist of a tricyclic lactone ring (ABC-ring) connected to a methylbutenolide (D-ring). Although it is known that 5DS biosynthesis begins with carlactonoic acid (CLA) derived from β-carotene, the enzyme that catalyzes the conversion of CLA remains elusive. Recently, we identified cytochrome P450 (CYP) CYP722C as the enzyme that catalyzes direct conversion of CLA to orobanchol in cowpea and tomato (Wakabayashi et al., Sci Adv 5:eaax9067, 2019). Orobanchol has a different C-ring configuration from that of 5DS. The present study aimed to characterize the homologous gene, designated GaCYP722C, from cotton (Gossypium arboreum) to examine whether this gene is involved in 5DS biosynthesis. Expression of GaCYP722C was upregulated under phosphate starvation, which is an SL-producing condition. Recombinant GaCYP722C was expressed in a baculovirus-insect cell expression system and was found to catalyze the conversion of CLA to 5DS but not to 4-deoxyorobanchol. These results strongly suggest that GaCYP722C from cotton is a 5DS synthase and that CYP722C is the crucial CYP subfamily involved in the generation of canonical SLs, irrespective of the different C-ring configurations.
Springer Science and Business Media LLC, 2020年05月, Planta, 251 (5), 97 - 97, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
Abstract Tomato plants (Solanum lycopersicum) contain steroidal glycoalkaloid α-tomatine, which functions as a chemical barrier to pathogens and predators. α-Tomatine accumulates in all tissues and at particularly high levels in leaves and immature green fruits. The compound is toxic and causes a bitter taste, but its presence decreases through metabolic conversion to nontoxic esculeoside A during fruit ripening. This study identifies the gene encoding a 23-hydroxylase of α-tomatine, which is a key to this process. Some 2-oxoglutarate-dependent dioxygenases were selected as candidates for the metabolic enzyme, and Solyc02g062460, designated Sl23DOX, was found to encode α-tomatine 23-hydroxylase. Biochemical analysis of the recombinant Sl23DOX protein demonstrated that it catalyzes the 23-hydroxylation of α-tomatine and the product spontaneously isomerizes to neorickiioside B, which is an intermediate in α-tomatine metabolism that appears during ripening. Leaves of transgenic tomato plants overexpressing Sl23DOX accumulated not only neorickiioside B but also another intermediate, lycoperoside C (23-O-acetylated neorickiioside B). Furthermore, the ripe fruits of Sl23DOX-silenced transgenic tomato plants contained lower levels of esculeoside A but substantially accumulated α-tomatine. Thus, Sl23DOX functions as α-tomatine 23-hydroxylase during the metabolic processing of toxic α-tomatine in tomato fruit ripening and is a key enzyme in the domestication of cultivated tomatoes.Oxford University Press (OUP), 2020年01月01日, Plant and Cell Physiology, 61 (1), 21 - 28[査読有り]
研究論文（学術雑誌）
α-tomatine and dehydrotomatine are steroidal glycoalkaloids (SGAs) that accumulate in the mature green fruits, leaves, and flowers of tomatoes (Solanum lycopersicum) and function as defensive compounds against pathogens and predators. The aglycones of α-tomatine and dehydrotomatine are tomatidine and dehydrotomatidine (5,6-dehydrogenated tomatidine), and tomatidine is derived from dehydrotomatidine via four reaction steps: C3 oxidation, isomerization, C5α reduction, and C3 reduction. Our previous studies (Lee et al. 2019) revealed that Sl3βHSD is involved in the three reactions except for C5α reduction, and in the present study, we aimed to elucidate the gene responsible for the C5α reduction step in the conversion of dehydrotomatidine to tomatidine. We characterized the two genes, SlS5αR1 and SlS5αR2, which show high homology with DET2, a brassinosteroid 5α reductase of Arabidopsis thaliana. The expression pattern of SlS5αR2 is similar to those of SGA biosynthetic genes, while SlS5αR1 is ubiquitously expressed, suggesting the involvement of SlS5αR2 in SGA biosynthesis. Biochemical analysis of the recombinant proteins revealed that both of SlS5αR1 and SlS5αR2 catalyze the reduction of tomatid-4-en-3-one at C5α to yield tomatid-3-one. Then, SlS5αR1- or SlS5αR2-knockout hairy roots were constructed using CRISPR/Cas9 mediated genome editing. In the SlS5αR2-knockout hairy roots, the α-tomatine level was significantly decreased and dehydrotomatine was accumulated. On the other hand, no change in the amount of α-tomatine was observed in the SlS5αR1-knockout hairy root. These results indicate that SlS5αR2 is responsible for the C5α reduction in α-tomatine biosynthesis and that SlS5αR1 does not significantly contribute to α-tomatine biosynthesis.
2019年12月25日, Plant biotechnology (Tokyo, Japan), 36 (4), 253 - 263, 英語, 国内誌[査読有り]
研究論文（学術雑誌）
- American Association for the Advancement of Science ({AAAS}), 2019年12月18日, Science Advances, 5 (12)
[査読有り]
研究論文（学術雑誌）
α-Tomatine and dehydrotomatine are major steroidal glycoalkaloids (SGAs) that accumulate in the mature green fruits, leaves and flowers of tomato (Solanum lycopersicum), and function as defensive compounds against bacteria, fungi, insects and animals. The aglycone of dehydrotomatine is dehydrotomatidine (5,6-dehydrogenated tomatidine, having the Δ5,6 double bond; the dehydro-type). The aglycone of α-tomatine is tomatidine (having a single bond between C5 and C6; the dihydro-type), which is believed to be derived from dehydrotomatidine via four reaction steps: C3 oxidation, isomerization, C5 reduction and C3 reduction; however, these conversion processes remain uncharacterized. In the present study, we demonstrate that a short-chain alcohol dehydrogenase/reductase designated Sl3βHSD is involved in the conversion of dehydrotomatidine to tomatidine in tomato. Sl3βHSD1 expression was observed to be high in the flowers, leaves and mature green fruits of tomato, in which high amounts of α-tomatine are accumulated. Biochemical analysis of the recombinant Sl3βHSD1 protein revealed that Sl3βHSD1 catalyzes the C3 oxidation of dehydrotomatidine to form tomatid-4-en-3-one and also catalyzes the NADH-dependent C3 reduction of a 3-ketosteroid (tomatid-3-one) to form tomatidine. Furthermore, during co-incubation of Sl3βHSD1 with SlS5αR1 (steroid 5α-reductase) the four reaction steps converting dehydrotomatidine to tomatidine were completed. Sl3βHSD1-silenced transgenic tomato plants accumulated dehydrotomatine, with corresponding decreases in α-tomatine content. Furthermore, the constitutive expression of Sl3βHSD1 in potato hairy roots resulted in the conversion of potato SGAs to the dihydro-type SGAs. These results demonstrate that Sl3βHSD1 is a key enzyme involved in the conversion processes from dehydrotomatidine to tomatidine in α-tomatine biosynthesis.
2019年06月01日, Plant and Cell Physiology, 60 (6), 1304 - 1315, 英語, 国内誌[査読有り]
研究論文（学術雑誌）
- Springer Science and Business Media LLC, 2019年03月, Nature Plants, 5 (3), 258 - 262, 英語
[査読有り]
研究論文（学術雑誌）
- Informa UK Limited, 2019年, Plant Signaling & Behavior, 15 (7), 1605810 - 1605810, 英語
[査読有り][招待有り]
研究論文（学術雑誌）
- 2018年12月, Journal of Pesticide Science, 43 (3), 207 - 213, 英語Transcriptome analysis of Arabidopsis thaliana treated with green leaf volatiles; possible role of green leaf volatiles as self-made damage-associated molecular patterns
[査読有り]
研究論文（学術雑誌）
- 2018年12月, Journal of Pesticide Science, 43 (3), 198 - 206, 英語The bioconversion of 5-deoxystrigol isomers to monohydroxylated strigolactones by plants
[査読有り]
研究論文（学術雑誌）
Reductive metabolism of strigolactones (SLs) in several plants was investigated. Analysis of aquaculture filtrates of cowpea and sorghum each fed with four stereoisomers of GR24, the most widely used synthetic SL, revealed stereospecific reduction of the double bond at C-3' and C-4' in the butenolide D-ring with preference for an unnatural 2'S configuration. The cowpea metabolite converted from 2'-epi-GR24 and the sorghum metabolite converted from ent-GR24 had the methyl group at C-4' in the trans configuration with the substituent at C-2', different from the cis configuration of the synthetic H2-GR24 reduced with Pd/C catalyst. The plants also reduced the double bond in the D-ring of 5-deoxystrigol isomers with a similar preference. The metabolites and synthetic H2-GR24 stereoisomers were much less active than were the GR24 stereoisomers in inducing seed germination of the root parasitic weeds Striga hermonthica, Orobanche crenata, and O. minor. These results provide additional evidence of the importance of the D-ring for bioactivity of SLs.
2018年08月, Bioorganic & medicinal chemistry, 26 (14), 4225 - 4233, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
- 2018年08月, Plant Physiology and Biochemistry, 131, 70 - 77, 英語Generation of -solanine-free hairy roots of potato by CRISPR/Cas9 mediated genome editing of the St16DOX gene
[査読有り]
研究論文（学術雑誌）
Strigolactones (SLs), comprising compounds with diverse but related chemical structures, are determinant signals in elicitation of germination in root parasitic Orobanchaceae and in mycorrhization in plants. Further, SLs are a novel class of plant hormones that regulate root and shoot architecture. Dissecting common and divergent biosynthetic pathways of SLs may provide avenues for modulating their production in planta. Biosynthesis of SLs in various SL-producing plant species was inhibited by fluridone, a phytoene desaturase inhibitor. The plausible biosynthetic precursors of SLs were exogenously applied to plants, and their conversion to canonical and non-canonical SLs was analysed using liquid chromatography-tandem mass spectrometry. The conversion of carlactone (CL) to carlactonoic acid (CLA) was a common reaction in all investigated plants. Sorghum converted CLA to 5-deoxystrigol (5-DS) and sorgomol, and 5-DS to sorgomol. One sorgomol-producing cotton cultivar had the same SL profile as sorghum in the feeding experiments. Another cotton cultivar converted CLA to 5-DS, strigol, and strigyl acetate. Further, 5-DS was converted to strigol and strigyl acetate. Moonseed converted CLA to strigol, but not to 5-DS. The plant did not convert 5-DS to strigol, suggesting that 5-DS is not a precursor of strigol in moonseed. Similarly, 4-deoxyorobanchol was not a precursor of orobanchol in cowpea. Further, sunflower converted CLA to methyl carlactonoate and heliolactone. These results indicated that the biosynthetic pathways of hydroxy SLs do not necessarily involve their respective deoxy SL precursors.
2018年04月, Journal of Experimental Botany, 69 (9), 2305 - 2318, 英語, 国際誌[査読有り]
研究論文（学術雑誌）
The obligate root parasitic Orobanchaceae plants Striga, Orobanche and Phelipanche spp. parasitize economically important crops, vegetables and oil plants. They are the most devastating agricultural weed pests worldwide. Based on an analysis of the climatic requirements of these parasites, very large areas of new territory are at risk of invasion if care is not taken. Recent research in combatting root parasitic weeds was reviewed based on scientific papers reported from 2010 and onwards. The countermeasures fell into eight kinds: resistant varieties, tolerant varieties, microbiological approach, cultural practices, chemical controls, host-induced gene silencing, integrated management and dissemination of technologies including the current situation survey. The development of practical, feasible and economical control technologies against root parasitic weeds would be expected by advancing and combining the countermeasures.
Taylor and Francis Ltd., 2018年03月04日, Biotechnology and Biotechnological Equipment, 32 (2), 221 - 240, 英語[査読有り][招待有り]
研究論文（学術雑誌）
- Oxford University Press (OUP), 2017年12月, Plant Physiology, 175, (1), 120 - 133, 英語
[査読有り]
研究論文（学術雑誌）
Light and temperature affect state transitions through changes in the plastoquinone (PQ) redox state in photosynthetic organisms. We demonstrated that light and/or heat treatment induced preferential photosystem (PS) I excitation by binding light-harvesting complex II (LHCII) proteins. The photosystem of wheat was in state 1 after dark overnight treatment, wherein PQ was oxidized and most of LHCII was not bound to PSI. At the onset of the light treatment [25 A degrees C in the light (100 A mu mol photons m(-2) s(-1))], two major LHCIIs, Lhcb1 and Lhcb2 were phosphorylated, and the PSI-LHCII supercomplex formed within 5 min, which coincided with an increase in the PQ oxidation rate. Heat treatment at 40 A degrees C of light-adapted wheat led to further LHCII protein phosphorylation of, resultant cyclic electron flow promotion, which was accompanied by ultrafast excitation of PSI and structural changes of thylakoid membranes, thereby protecting PSII from heat damage. These results suggest that LHCIIs are required for the functionality of wheat plant PSI, as it keeps PQ oxidized by regulating photochemical electron flow, thereby helping acclimation to environmental changes.
SPRINGER, 2017年01月, Photosynthesis Research, 131 (1), 41 - 50, 英語[査読有り]
研究論文（学術雑誌）
Root parasitic weeds, such as Orobanche spp. and Striga spp., cause serious damage to crops. Recently, it was demonstrated that nojirimycin (NJ) selectively inhibits seed germination in these weeds. In this study, we modified the medium for Streptomyces ficellus to increase its production of NJ and evaluated the culture as an antiparasitic weed agent. We screened alternatives to Pharmamedia'', an additive in the original medium, and found that marine broth stimulated NJ production. Moreover, soluble starch-depleted medium could maintain S. ficellus NJ production. The NJ concentration reached 710 mg/L after four-day batch culture in starch-depleted marine broth medium, which was 17-fold higher than that in the Pharmamedia" medium. The culture in the marine broth medium inhibited seed germination of Orobanche spp. and Striga spp. as effectively as a standard solution of NJ. Pesticide Science Society of Japan
PESTICIDE SCI SOC JAPAN, 2017年, JOURNAL OF PESTICIDE SCIENCE, 42 (3-4), 166 - 171, 英語[査読有り]
研究論文（学術雑誌）
Fifteen steroidal saponins 1–15, which include 4 furostanol glycosides 1–3 and 15, and 11 spirostanol glycosides 4–14, were isolated from the tubers and leaves of lesser yam (Dioscorea esculenta, Togedokoro). Their structures were identified by nuclear magnetic resonance and liquid chromatography mass spectroscopy. Four steroidal saponins 9, 11, 14, and 15 were found to be novel compounds.
Japan Society for Bioscience Biotechnology and Agrochemistry, 2017年, Bioscience, Biotechnology and Biochemistry, 81 (12), 2253 - 2260, 英語[査読有り]
研究論文（学術雑誌）
- 2016年10月, Pest Management Science, 72 (11), 2035 - 2042, 英語Practicality of suicidal germination induction for controlling Striga hermonthica
[査読有り]
研究論文（学術雑誌）
The green odor of plants is characterized by green leaf volatiles (GLVs) composed of C6 compounds. GLVs are biosynthesized from polyunsaturated fatty acids in thylakoid membranes by a series of enzymes. A representative member of GLVs (E)-2-hexenal, known as the leaf aldehyde, has been assumed to be produced by isomerization from (Z)-3-hexenal in the biosynthesis pathway; however, the enzyme has not yet been identified. In this study, we purified the (Z)-3:(E)-2-hexenal isomerase (HI) from paprika fruits and showed that various plant species have homologous HIs. Purified HI is a homotrimeric protein of 110 kDa composed of 35-kDa subunits and shows high activity at acidic and neutral pH values. Phylogenetic analysis showed that HIs belong to the cupin superfamily, and at least three catalytic amino acids (His, Lys, Tyr) are conserved in HIs of various plant species. Enzymatic isomerization of (Z)-3-hexenal in the presence of deuterium oxide resulted in the introduction of deuterium at the C4 position of (E)-2-hexenal, and a suicide substrate 3-hexyn-1-al inhibited HI irreversibly, suggesting that the catalytic mode of HI is a keto-enol tautomerism reaction mode mediated by a catalytic His residue. The gene expression of HIs in Solanaceae plants was enhanced in specific developmental stages and by wounding treatment. Transgenic tomato plants overexpressing paprika HI accumulated (E)-2-hexenal in contrast to wild-type tomato plants mainly accumulating (Z)-3-hexenal, suggesting that HI plays a key role in the production of (E)-2-hexenal in planta.
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2016年07月, JOURNAL OF BIOLOGICAL CHEMISTRY, 291 (27), 14023 - 14033, 英語[査読有り]
研究論文（学術雑誌）
Rice has become a major staple cereal in sub-Saharan Africa. Currently, upland rice cultivation is expanding particularly in rainfed areas where the root parasitic weed Striga hermonthica, a major constraint to cereal production, is endemic. Laboratory, pot, and semi-controlled open air experiments were performed to evaluate resistance of selected rice varieties in Sudan to a resident S. hermonthica population. In the laboratory, 27 varieties were screened for post-attachment resistance using the rhizotron technique. Varieties displaying high post attachment resistance, Umgar, NERICA5, and NERICA13 together with NERICA4, NERICA18, and Nipponbare, a lowland rice variety, were further evaluated for performance and Striga resistance in pot and semi controlled open air experiments and for germination inducing activity in a laboratory. In addition, comparative studies on reaction of Umgar, Kosti1 and Kosti2, released varieties for commercial production in Sudan, to the parasite were performed in two pot experiments. In the pot experiments Umgar and NERICA5, consistently, sustained the lowest Striga emergence (<2.2 Striga plants per pot), while NERICA13 and NERICA4 supported 1.8-5.7 and 8.7-16.4 Striga plants per pot, respectively. In an artificially Striga-infested field, number of emergent Striga plants per 10 rice hills, at harvest, was 2.0, 2.0, 4.8, 13.5, 13.3, and 18.3 on Umgar, NERICA5, NERICA13, NERICA4, NERICA18, and Nipponbare, respectively. Striga had no adverse effects on total above-ground parts and panicle dry weight in Umgar and NERICA5. Germination-inducing activity of root exudates, at 14 days after sowing onward, was markedly lower for Umgar than for NERICA5, NERICA13, NERICA4, and NERICA18. Based on these findings, Umgar has both pre and post-attachment resistance to a resident Striga population in Sudan. Kostil and Kosti2 did not exhibit Striga-resistance at the same level as Umgar. Further the resistance of NERICA5, a variety reported to be endowed with a broad spectrum resistance to Striga species and ecotypes, at least to one resident Striga population in Sudan was clearly indicated.
FRONTIERS MEDIA SA, 2016年05月, FRONTIERS IN PLANT SCIENCE, 7, 634, 英語[査読有り]
研究論文（学術雑誌）
Three known iridoid glucosides (gentiournoside A, gentiournoside E and depressoside) were isolated from the flowers of Gentiana urnula Harry Sm. through activity-guided fractionations with a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. All three compounds exhibited excellent DPPH radical scavenging activities (IC50: 10-20molL(-1)) comparable to that of ascorbic acid and Trolox. However, examination of the NMR data revealed that the reported chemical structure of depressoside, previously isolated from the leaves of G. depressa, needed correcting due to incorrect elucidation around C-7 of the iridane skeleton, and was corrected to 6--(2,3-dihydroxyphenyl)-d-glucosyl 7-O-(2,3-dihydroxybenzoyl)-loganate. Depressoside exhibited a much higher scavenging activity against superoxide radicals (IC50: 45.5molL(-1)) than the other two extracted compounds (IC50: more than 900molL(-1)) due to the crucial presence of a pyrogallyl unit.
TAYLOR & FRANCIS LTD, 2016年04月, NATURAL PRODUCT RESEARCH, 30 (8), 954 - 959, 英語[査読有り]
研究論文（学術雑誌）
- 公益財団法人 日本学術協力財団, 2016年, 学術の動向, 21 (8), 8_18 - 8_23, 日本語
- 日本作物学会, 2016年, 日本作物学会講演会要旨集, 241, 25 - 25, 日本語
Steroidal saponins are natural surfactants with various biological activities, and the tubers of Dioscorea, known as yam, contain a furostanol glycoside protodioscin and a spirostanol glycoside dioscin, which are valuable saponins required for semi-synthetic production of pharmaceutical steroidal drugs. Steroidal saponins are biosynthesized from cholesterol via several steps of oxygenation and transglycosylation, and a beta-glucosidase is involved in the hydrolytic conversion from furostanol glycosides to spirostanol glycosides. To investigate steroidal saponin biosynthesis in Dioscorea spps, comparative transcriptome analysis of high saponin producers, D. esculenta and D. cayenensis, and a low producer, D. alata, was performed using 454 pyrosequencing. In this study, we isolated and characterized a beta-glucosidase ( DeF26G1) from D. esculenta. The DeF26G1 cDNA encodes a family 1 glucosidase, and the DeF26G1 transcript was present at high levels in D. esculenta but not detected in D. alata. The recombinant DeF26G1 protein hydrolyzed the 26-O-glycosidic bond of protodioscin to form dioscin, indicating that the DeF26G1 gene encodes furostanol glycoside 26-O-beta-glucosidase. These results suggested that DeF26G1 is involved in the conversion of furostanol saponins to spirostanol saponins, which seems to be related to biological defense response in the leaves of Dioscorea plants.
The Japanese Society for Plant Cell and Molecular Biology, 2015年12月, Plant Biotechnology, 32 (4), 299 - U141, 英語[査読有り]
研究論文（学術雑誌）
Alectrol was first isolated from root exudates of cowpea (Vigna unguiculata), a genuine host of a root parasitic weed Striga gesnerioides, as a germination stimulant for seeds of the parasite. The proposed structure, an isomer of strigol, was disproven by chemical synthesis. Recently, another structure, namely orobanchyl acetate, was proposed. Surprisingly, however, the synthetic compound having this proposed structure for alectrol was not active in inducing germination of S. gesnerioides seeds although it was active toward seeds of other root parasitic weeds such as S. hermonthica and Orobanche minor. Detailed studies on H-1 NMR, mass and CD spectra of naturally occurring alectrol, re-isolated from cowpea root exudates, revealed that the genuine structure of the germination stimulant is not orobanchyl acetate but its stereoisomer ent-2'-epi-orobanchyl acetate. Accordingly, the structure of natural orobanchol was revised to ent-2'-epi-orobanchol 12 years after a tentative structure of orobanchol was proposed. Strict stereochemical requirements of strigolactones for germination induction of S. gesnerioides seeds, authentic samples of synthetic strigolactones and advanced analytical instruments made the structural assignment possible, thus ending a 20 years controversy concerning the true structure of alectrol.
SPRINGER, 2015年10月, PHYTOCHEMISTRY REVIEWS, 14 (5), 835 - 847, 英語[査読有り]
研究論文（学術雑誌）
Bioconversion of GR24, the most widely used synthetic strigolactone (SL), by hydroponically grown sorghum (Sorghum bicolor) and biological activities of hydroxylated GR24 stereoisomers were studied. Analysis of extracts and exudates of sorghum roots previously fed with a racemic and diastereomeric mixture of GR24, using liquid chromatography-tandem mass spectrometry with multiple reaction monitoring (MRM), confirmed uptake of GR24 and suggested its conversion to mono-hydroxylated products. Two major GR24 metabolites, 7-hydroxy-GR24 and 8-hydroxy-GR24, were identified in the root extracts and exudates by direct comparison of chromatographic behavior with a series of synthetic mono-hydroxylated GR24 analogues. Separate feeding experiments with each of the GR24 stereoisomers revealed that the hydroxylated products were derived from 2'-epi-GR24, an evidence of sterical recognition of the GR24 molecule by sorghum. Trans-4-hydroxy-GR24 isomers derived from all GR24 stereoisomers were detected in the exudates as minor metabolites. The synthetic hydroxy-GR24 isomers induced germination of Striga hermonthica in decreasing order of C-8 > C-7 > C-6 > C-5 > C-4. In contrast the stereoisomers having the same configuration of orobanchol, irrespective of position of hydroxylation, induced germination of Striga gesnerioides. The results confirm previous reports on structural requirements of SLs and ascribe a critical role to hydroxylation, but not to the position of the hydroxyl group in the AB part of the molecule, in induction of S. gesnerioides seed germination. (c) 2015 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2015年09月, BIOORGANIC & MEDICINAL CHEMISTRY, 23 (18), 6100 - 6110, 英語[査読有り]
研究論文（学術雑誌）
- 2015年06月, J. Exp. Bot., 英語Identification of planteose as a storage carbohydrate for seed germination of Orobanche minor and elucidation of its metabolism as a possible target for selective control
[査読有り]
研究論文（学術雑誌）
- Oxford University Press, 2015年03月28日, Journal of Experimental Botany, 66 (11), 3129 - 3140, 英語
[査読有り]
研究論文（学術雑誌）
- 一般社団法人 植物化学調節学会, 2015年, 植物化学調節学会 研究発表記録集, 50, 68 - 68, 日本語
- 日本作物学会, 2015年, 日本作物学会講演会要旨集, 240, 107 - 107, 日本語
Abiotic stresses cause serious damage to plants; therefore, plants undergo a complicated stress response through signal transduction originating from environmental stimuli. Here we show that a subset of short-chain leaf volatiles with an alpha, beta-unsaturated carbonyl bond in their structure (reactive short-chain leaf volatiles, RSLVs) like (E)-2-hexenal and (E)-2-butenal can act as signal chemicals that strongly induce the gene expression of abiotic-related transcription factors, such as heat stress-related transcription factors (HSFA2, MBF1c) and other abiotic stress-related transcription factors (DREB2A, ZATs). RSLV-induced expression of HSFA2 and MBF1c was eliminated in HSFA1s-, known as heat stress response master regulators, knockout mutant, whereas those of DREB2A and ZATs were not, suggesting that the RSLV signaling pathway is composed of HSFA1-dependent and -independent pathways. RSLV treatment induced production of chaperon proteins, and the RSLV-treated Arabidopsis thus demonstrated enhanced abiotic stress tolerance. Because oxidative stress treatment enhanced RSLV production, we concluded that commonly found RSLVs produced by environmental stresses are powerful inducer of abiotic stress-related gene expression as oxidative stress signals.
NATURE PUBLISHING GROUP, 2015年01月, SCIENTIFIC REPORTS, 5, 英語[査読有り]
研究論文（学術雑誌）
Photosystems of higher plants alleviate heat-induced damage in the presence of light under moderate stressed conditions; however, in the absence of light (i.e., in the dark), the same plants are damaged more easily. (Yamauchi and Kimura, 2011) We demonstrate that regulating photochemical energy transfer in heat-treated wheat at 40 degrees C with light contributed to heat tolerance of the photosystem. Chlorophyll fluorescence analysis using heat-stressed wheat seedlings in light showed increased non-photochemical quenching (NPQ) of chlorophyll fluorescence, which was due to thermal dissipation that was increased by state 1 to state 2 transition. Transmission electron microscopy revealed structural changes in thylakoid membranes, including unstacking of grana regions under heat stress in light. It was accompanied by the phosphorylation of thylakoid proteins such as D1 and D2 proteins and the light harvesting complex II proteins Lhcb1 and Lhcb2. These results suggest that heat stress at 40 degrees C in light induces state 1 to state 2 transition for the preferential excitation of photosystem I (PSI) by phosphorylating thylakoid proteins more strongly. Structural changes of thylakoid membrane also assist the remodeling of photosystems and regulation of energy distribution by transition toward state 2 probably contributes to plastoquione oxidation; thus, light-driven electrons flowing through PSI play a protective role against PSII damage under heat stress.
MDPI AG, 2014年12月, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 15 (12), 23042 - 23058, 英語[査読有り]
研究論文（学術雑誌）
Root exudates of sunflower (Helianthus annuus L.) line 2607A induced germination of seeds of root parasitic weeds Striga hermonthica, Orobanche cumana, Orobanche minor, Orobanche crenata, and Phelipanche aegyptiaca. Bioassay-guided purification led to the isolation of a germination stimulant designated as heliolactone. FT-MS analysis indicated a molecular formula of C20H24O6. Detailed NMR spectroscopic studies established a methylfuranone group, a common structural component of strigolactones connected to a methyl ester of a C-14 carboxylic acid via an enol ether bridge. The cyclohexenone ring is identical to that of 3-oxo-alpha-ionol and the other part of the molecule corresponds to an oxidized carlactone at C-19. It is a carlactone-type molecule and functions as a germination stimulant for seeds of root parasitic weeds. Heliolactone induced seed germination of the above mentioned root parasitic weeds, while dehydrocostus lactone and costunolide, sesquiterpene lactones isolated from sunflower root exudates, were effective only on O. cumana and O. minor. Heliolactone production in aquacultures increased when sunflower seedlings were grown hydroponically in tap water and decreased on supplementation of the culture with either phosphorus or nitrogen. Costunolide, on the other hand, was detected at a higher concentration in well-nourished medium as opposed to nutrient-deficient media, thus suggesting a contrasting contribution of heliolactone and the sesquiterpene lactone to the germination of O. cumana under different soil fertility levels. (c) 2014 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2014年12月, PHYTOCHEMISTRY, 108, 122 - 128, 英語[査読有り]
研究論文（学術雑誌）
- 2014年08月10日, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 248Study on the effect of nojirimycin on sugar metabolism in germinating seeds for selective control of root parasitic weeds
[査読有り]
The gas exchange of the upper fully expanded leaf of the root parasite Striga hermonthica and of its host Sorghum bicolor was measured under wet and dry conditions to identify the mechanisms of the devastating effects of the parasite on its hosts under drought. The short-term water stress severely reduced photosynthetic rate in infected sorghum, but less in S. hermonthica. Soil water stress did not affect leaf respiration rate in either S. hermonthica or infected sorghum. This suggests that under dry conditions both infected sorghum and S. hermonthica decreased autotrophic carbon gain. The transpiration rate of S. hermonthica, a major driving force for assimilate uptake from the host, was higher and less affected by water stress than that of infected sorghum. Stomatal density on the abaxial surfaces of the leaves was higher in S. hermonthica than in sorghum. Both S. hermonthica infection and water stress decreased stomatal conductance of the sorghum leaves. S. hermonthica, irrespective of soil water status, had greater stomatal aperture on the adaxial and abaxial surfaces of its leaves than infected sorghum. These results indicate that the higher transpiration rate of S. hermonthica even under water stress, achieved through higher stomatal density on the abaxial surfaces of the leaves and greater stomatal aperture on both surfaces of the leaves, may induce the maintenance of water and solute transfers from the host to the parasite leading to severe damage to the host under drought. © 2013 Springer Science+Business Media Dordrecht.
2013年12月, Biologia Plantarum, 57 (4), 773 - 777, 英語[査読有り]
研究論文（学術雑誌）
Key message: Structure-activity relationship studies of strigolactones and Striga gesnerioides seed germination revealed strict structural requirements for germination induction and a new function of the plant hormones as germination inhibitors. Stereoisomers of the naturally occurring strigolactones, strigol, sorgolactone, orobanchol, sorgomol and 5-deoxystrigol, 36 in total, were prepared and screened for the ability to induce and/or inhibit the germination of Striga hermonthica and Striga gesnerioides seeds collected from mature plants that parasitized on sorghum and cowpea, respectively. All of the compounds induced S. hermonthica seed germination, albeit displayed differential activities. On the other hand, only a limited number of the compounds induced significant germination in S. gesnerioides, thus indicating strict structural requirements. Strigolactones inducing high germination in S. gesnerioides induced low germination in S. hermonthica. Strigolactones with the same configuration at C3a, C8b and C2′ as that in 5-deoxystrigol (9a) induced high germination of S. hermonthica seeds, but most of them inhibited the germination of S. gesnerioides. The differential response of S. gesnerioides to strigolactones may play an important role in the survival of the species. However, the compounds could be used as means of control if mixed cropping of cowpea and sorghum is adopted. © 2013 Springer-Verlag Berlin Heidelberg.
2013年06月, Plant Cell Reports, 32 (6), 829 - 838, 英語[査読有り]
研究論文（学術雑誌）
A straightforward synthesis of 7-oxo-5-deoxystrigol, a 7-oxygenated strigolactone analog, was achieved by starting from 2,2-dimethylcyclohexane-1,3-dione.
TAYLOR & FRANCIS LTD, 2013年04月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77 (4), 832 - 835, 英語[査読有り]
研究論文（学術雑誌）
Strigolactones, important rhizosphere signalling molecules and a class of phytohormones that control shoot architecture, are apocarotenoids of plant origin. They have a structural core consisting of a tricyclic lactone connected to a butyrolactone group via an enol ether bridge. Deuterium-labelled 5-deoxystrigol stereoisomers were administered to aquacultures of a high sorgomol-producing sorghum cultivar, Sorghum bicolor (L.) Moench, and conversion of these substrates to sorgomol stereoisomers was investigated. Liquid chromatography-mass spectrometry analyses established that 5-deoxystrigol (5-DS) and ent-2′-epi-5-deoxystrigol were absorbed by sorghum roots, converted to sorgomol and ent-2′-epi-sorgomol, respectively, and exuded out of the roots. The conversion was inhibited by uniconazole-P, implying the involvement of cytochrome P450 in the hydroxylation. These results provide experimental evidence for the postulated biogenetic scheme for formation of strigolactones, in which hydroxylation at C-9 of 5-DS can generate sorgomol. © 2013 Elsevier B.V.
Elsevier Ltd, 2013年, Phytochemistry, 93, 41 - 48, 英語[査読有り]
研究論文（学術雑誌）
Under a moderately heat-stressed condition, the photosystems of higher plants are damaged in the dark more easily than they are in the presence of light. To obtain a better understanding of this heat-derived damage mechanism that occurs in the dark, we focused on the involvement of the light-independent electron flow that occurs at 40 A degrees C during the damage. In various plant species, the maximal photochemical quantum yield of photosystem (PS) II (F (v)/F (m)) decreased as a result of heat treatment in the dark. In the case of wheat, the most sensitive plant species tested, both F (v)/F (m) and oxygen evolution rapidly decreased by heat treatment at 40 A degrees C for 30 min in the dark. In the damage, specific degradation of D1 protein was involved, as shown by immunochemical analysis of major proteins in the photosystem. Because light canceled the damage to PSII, the light-driven electron flow may play a protective role against PSII damage without light. Light-independent incorporation of reducing power from stroma was enhanced at 40 A degrees C but not below 35 A degrees C. Arabidopsis mutants that have a deficit of enzymes which mediate the incorporation of stromal reducing power into thylakoid membranes were tolerant against heat treatment at 40 A degrees C in the dark, suggesting that the reduction of the plastoquinone pool may be involved in the damage. In conclusion, the enhanced introduction of reducing power from stroma into thylakoid membranes that occurs around 40 A degrees C causes over-reduction of plastoquinone, resulting in the damage to D1 protein under heat stress without linear electron flow.
SPRINGER, 2012年08月, PLANTA, 236 (2), 753 - 761, 英語[査読有り]
研究論文（学術雑誌）
- 2012年05月, Plant J., 70 (3), 460-470,, 英語A 2-oxoglutarate-dependent dioxygenase from Ruta graveolens L. exhibits p-coumaroyl-coA 2’-hydroxylase activity (C2’H), a missing step in the synthesis of umbelliferone in plants
[査読有り]
研究論文（学術雑誌）
- 2012年04月, FEBS Letters,, 586, 1208-1213,, 英語Chloroplastic NADPH-dependent alkenal/one oxidoreductase contributes to detoxification of reactive carbonyls originated from photosystem I under oxidative stress
[査読有り]
研究論文（学術雑誌）
- 日本作物学会, 2012年03月29日, 日本作物學會紀事, 81, 34 - 35, 日本語
- 2012年, Recent Research Developments in Phytochemistry,, 10, 1 - 12, 英語Germination response of Striga hermonthica and Orobanche minor seeds pre-treated with the synthetic strigolactone GR24
[査読有り]
研究論文（学術雑誌）
Modification of internal configuration of a bubble column, airlift and stirred tank reactor (10-200 L) was made for root cultures of Bupleurum falcatum L Agitation with an impeller covered with partition mesh was ineffective for a 10-L modified reactor, because it caused intensive foaming and subsequent overflow of the culture medium even at a low rotation speed of 50 rpm and a low aeration rate of 0.1 vvm (volume per volume of medium). In contrast, efficient aeration through a ceramic sparger placed at the bottom of a 20-L bubble column reactor yielded approximately 25 g/L of dry roots and 500 mg/L of sailcosaponin-a and saikosaponin-d over 42 days. On a 200-L scale, however, the roots became flocculated under the upper perforated plate initially positioned near the middle of the reactor, forming a firm disk of roots and a large empty space between the disk and the medium. Thus, the roots had poor contact with the medium, which severely suppressed their growth. To avoid this flocculation, a bottom perforated plate and draft tube were installed as a partitioning device separating the culturing area (outside the draft tube) from the aeration area (inside the draft tube). The draft tube was made of a stainless steel mesh rather than a solid material, and the tube greatly increased the root yield in the 20-L reactor. This configuration was successfully applied at the 200-L scale, yielding 500-600 mg/L of saikosaponin-a and saikosaponin-d over 56 days. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113 (1), 99 - 105, 英語[査読有り]
研究論文（学術雑誌）
Striga gesnerioides is a root parasitic weed of economic significance to cowpea ( Vigna unguiculata) crops in Western Africa. Seeds of the parasite germinate in response to cowpea root exudates. Germination stimulants for the seeds were isolated from the hydroponic culture filtrate of cowpea, and their structures were unambiguously determined as (-)-(3aR,412,8bR,2'R)-ent-2'-epi-orobanchol and (+)-(3aR,4R,8bR,2'R)-ent-2'-epi-orobanchyl acetate, on the basis of mass, CD, and (1)H NMR spectra; optical rotatory power; and chromatographic behavior on HPLC. The alcohol was first isolated and identified from the cowpea root exudates, and the acetate maybe the same compound that had been previously isolated from the exudates and designated as alectrol. Identity of the stimulants produced by cowpea to those produced by red clover (Trifolium pratense) was confirmed.
AMER CHEMICAL SOC, 2011年10月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59 (19), 10485 - 10490, 英語[査読有り]
研究論文（学術雑誌）
Strigolactones are highly potent germination stimulants for seeds of the parasitic weeds Striga and Orobanche spp., which severely reduce the yield of economically important crops in tropical and semitropical areas. GR24 (1) is a widely used synthetic strigolactone able to stimulate parasitic seed germination in the absence of a host plant to reduce soil seed levels. However, S. gesnerioides seeds do not respond to GR24 and only germinate by exposure to cowpea (Vigna unguiclata) root exudates or their active ingredient, the structure of which has not been established. Our present study provides substantial insight into the plant metabolism and structural requirements of strigolactones for host recognition by parasitic weeds. First, we clarified the structural and stereochemical requirements of synthetic strigolactones for seed germination of S. gesnerioides through bioassays of 4-hydroxy-GR24 (HO-GR24, 2) and 4-acetoxy-GR24 (AcO-GR24, 3) and their stereoisomers. These results suggest that both an oxygenated substituent at C-4 and the configuration of the tricyclic lactone and the D-ring are essential structural requirements for induction of S. gesnerioides seed germination. Furthermore, GR24 exhibited inhibitory activity against seed germination of S. gesnerioides at concentrations that induce seed germination of S. hermonthica and 0. minor. Second, we isolated the germination stimulants from hydroponic culture filtrate of cowpea, and their structures were unambiguously determined as (-)-(3aR,4R,8bR,2'R)-ent-2'-epi-orobanchol (11) and (+)-(3aR,4R,8bR,2'R)-ent-2'-epi-orobanchyl acetate (12), on the basis of mass, CD, and ^1H NMR spectra; optical rotator power; and chromatographic behavior on HPLC. These compounds are identical to products produced by red clover (Trifolium. pratense).
天然有機化合物討論会, 2011年09月02日, 天然有機化合物討論会講演要旨集, (53), 313 - 318, 日本語Abstract Plants are often exposed to temperatures of around 40^o^C. These temperatures can cause serious damage to photosystems, yet plants can survive with minimum damage. Here, we show that plants switch photosystem to protect photosystem II (PSII) at 40^o^C. Using wheat and Arabidopsis seedlings, we investigated the mechanisms of heat-derived damage in the dark and avoidance of damage in the light. Heat treatment at 40^o^C in the dark caused serious damage to PSII: the maximum quantum yield of PSII (Fv/Fm) and oxygen evolution rapidly decreased. The damage was due to the degradation of the D1 protein (shown by immuno-chemical analysis) and the disturbance of energy transfer in PSII core chlorophyll-binding proteins CP43 and CP47 (shown by time-resolved fluorescence measurement). The damage to PSII might be due to enhanced introduction of electrons from the reducing power of the stroma into thylakoid membranes, causing subsequent electron backflow to PSII. Plants treated at 40^o^C in the light avoided PSII damage and showed preferential excitation of photosystem I (PSI), phosphorylation and migration of light-harvesting complex II (LHCII), which indicate state transition of the photosystem to enhance thermal dispersion and light-driven cyclic electron flow around PSI. These results suggest that heat damage to PSII is probably due to a backflow of reducing power from the stroma to PSII, and that light causes a state transition of photosystem, driving cyclic electron flow and thus protecting PSII from damage.Springer Science and Business Media LLC, 2011年08月01日, Nature Precedings研究論文（学術雑誌）
- 2011年08月, J. Agric. Food Chem., 59, 9226-31, 英語Structural requirements of strigolactones for germination induction of Striga gesnerioides
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研究論文（学術雑誌）
Reactive carbonyls, especially alpha,beta-unsaturated carbonyls produced through lipid peroxidation, damage biomolecules such as proteins and nucleotides; elimination of these carbonyls is therefore essential for maintaining cellular homeostasis. In this study, we focused on an NADPH-dependent detoxification of reactive carbonyls in plants and explored the enzyme system involved in this detoxification process. Using acrolein (CH(2) = CHCHO) as a model alpha,beta-unsaturated carbonyl, we purified a predominant NADPH-dependent acrolein-reducing enzyme from cucumber leaves, and we identified the enzyme as an alkenal/one oxidoreductase (AOR) catalyzing reduction of an alpha,beta-unsaturated bond. Cloning of cDNA encoding AORs revealed that cucumber contains two distinct AORs, chloroplastic AOR and cytosolic AOR. Homologs of cucumber AORs were found among various plant species, including Arabidopsis, and we confirmed that a homolog of Arabidopsis (At1g23740) also had AOR activity. Phylogenetic analysis showed that these AORs belong to a novel class of AORs. They preferentially reduced alpha,beta-unsaturated ketones rather than alpha,beta-unsaturated aldehydes. Furthermore, we selected candidates of other classes of enzymes involved in NADPH-dependent reduction of carbonyls based on the bioinformatic information, and we found that an aldo-keto reductase (At2g37770) and aldehyde reductases (At1g54870 and At3g04000) were implicated in the reduction of an aldehyde group of saturated aldehydes and methylglyoxal as well as alpha,beta-unsaturated aldehydes in chloroplasts. These results suggest that different classes of NADPH-dependent reductases cooperatively contribute to the detoxification of reactive carbonyls.
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2011年03月, JOURNAL OF BIOLOGICAL CHEMISTRY, 286 (9), 6999 - 7009, 英語[査読有り]
研究論文（学術雑誌）
- 2011年01月, Tetrahedron Letters, 52, 724-726, 英語First synthesis of (±)-sorgomol, the germination stimulant for root parasitic weeds isolated from Sorghum bicolour
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研究論文（学術雑誌）
- 2010年10月, Journal of Japanese Society of Agricultural Technology Management, 17(2), 47-52, 英語Use of an acoustic emission sensor method to non-destructively evaluate crop root dynamics.
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研究論文（学術雑誌）
The root parasitic plant, Striga hermonthica, constrains the production of several agronomically important poaceous crops in the arid and semiarid tropical regions of Sub-Saharan Africa. The parasite is incompatible with the model legume, Lotus japonicus. Studies at the molecular and metabolic levels have revealed that expression of the genes involved in the biosynthesis of vestitol, a legume-specific phytoalexin, was highly up-regulated in L. japonicus roots challenged with S. hermonthica. High-performance liquid chromatography and mass spectroscopy confirmed the presence of vestitol in the exudate released from L. japonicus roots inoculated with S. hermonthica seedlings. Fluorescence, similar to that emitted by authentic vestitol, was displayed on the surface of L. japonicus roots to which successful attachment of S. hermonthica had been achieved. Vestitol exerted a limited inhibitory effect on S. hermonthica germination, but it significantly inhibited seedling growth. These results indicate that vestitol biosynthesis in L. japonicus was induced by S. hermonthica attachment and that vestitol contributed, at least in part, to the host's defence mechanism and acted as a chemical barrier against the intrusion of the parasite.
TAYLOR & FRANCIS LTD, 2010年08月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74 (8), 1662 - 1667, 英語[査読有り]
研究論文（学術雑誌）
Previously we observed that the oxygen-evolving complex 33 kDa protein (OEC33) which stabilizes the Mn cluster in photosystem II (PSII), was modified with malondialdehyde (MDA), an end-product of peroxidized polyunsaturated fatty acids, and the modification increased in heat-stressed plants (Yamauchi et al. 2008). In this study, we examined whether the modification of OEC33 with MDA affects its binding to the PSII complex and causes inactivation of the oxygen-evolving complex. Purified OEC33 and PSII membranes that had been removed of extrinsic proteins of the oxygen-evolving complex (PSIIa dagger OEE) of spinach (Spinacia oleracea) were separately treated with MDA. The binding was diminished when both OEC33 and PSIIa dagger OEE were modified, but when only OEC33 or PSIIa dagger OEE was treated, the binding was not impaired. In the experiment using thylakoid membranes, release of OEC33 from PSII and corresponding loss of oxygen-evolving activity were observed when thylakoid membranes were treated with MDA at 40A degrees C but not at 25A degrees C. In spinach leaves treated at 40A degrees C under light, maximal efficiency of PSII photochemistry (F (v)/F (m) ratio of chlorophyll fluorescence) and oxygen-evolving activity decreased. Simultaneously, MDA contents in heat-stressed leaves increased, and OEC33 and PSII core proteins including 47 and 43 kDa chlorophyll-binding proteins were modified with MDA. In contrast, these changes were to a lesser extent at 40A degrees C in the dark. These results suggest that MDA modification of PSII proteins causes release of OEC33 from PSII and it is promoted in heat and oxidative conditions.
SPRINGER, 2010年04月, PLANTA, 231 (5), 1077 - 1088, 英語[査読有り]
研究論文（学術雑誌）
- 2010年, Bioscience Research, 7(1): 26-31,, 英語Effect of NaCl on Orobanche spp and Striga hermonthica seeds germination during and after conditioning. Bioscience Research
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研究論文（学術雑誌）
- 2009年05月, Tetrahedron Letters, 50, 4549-4551Synthetic disproof of the structure proposed for solanacol, the germination stimulant for seeds of root parasitic weeds
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研究論文（学術雑誌）
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.
OXFORD UNIV PRESS, 2009年02月, JOURNAL OF EXPERIMENTAL BOTANY, 60 (2), 641 - 650, 英語[査読有り]
研究論文（学術雑誌）
Dechlorodauricumine (5) and dechloroacutumine (6) were converted to miharumine (7) and dechloroacutumidine (8), respectively, by a cell-free preparation from cultured roots of Menispermum dauricum in the presence of FAD. The structures of 7 and 8 were elucidated on the basis of spectroscopic analyses and chemical conversion.
TAYLOR & FRANCIS LTD, 2009年02月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73 (2), 440 - 442, 英語[査読有り]
研究論文（学術雑誌）
Witchweeds (Striga spp.) and broomrapes (Orobanche spp.) are obligate root parasitic plants on economically important field and horticultural crops. The parasites' seeds are induced to germinate by root-derived chemical signals. The radicular end is transformed into a haustorium which attaches, penetrates the host root and establishes connection with the vascular system of the host. Reactions of Lotus japonicus, a model. legume for functional genomics, were studied for furthering the understanding of host-parasite interactions. Lotus japonicus was compatible with Orobanche aegyptiaca, but not with Orobanche minor, Striga hermonthica and Striga gesnerioides. Orobanche minor successfully penetrated Lotus japonicus roots, but failed to establish connections with the vascular system. Haustoria in Striga hermonthica attached to the roots, but penetration and subsequent growth of the endophyte in the cortex were restricted. Striga gesnerioides did not parasitize Lotus japonicus. Among seven mutants of Lotus japonicus (castor-5, har1-5, alb1-1, ccamk-3, nup85-3, nfr1-3 and nsp2-1) with altered characteristics in relation to rhizobial nodulation and mycorrhizal colonization, castor-5 and har1-5 were parasitized by Orobanche aegyptiaca with higher frequency than the wild type. In contrast, Orobanche aegyptiaca tubercle development was delayed on the mutants nup85-3, nfr1-3 and nsp2-1. These results suggest that nodulation, mycorrhizal colonization and infection by root parasitic plants in Lotus japonicus may be modulated by similar mechanisms and that Lotus japonicus is a potential model, legume for studying plant-plant parasitism. (C) 2008 Elsevier GmbH. All rights reserved.
ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2009年, JOURNAL OF PLANT PHYSIOLOGY, 166 (4), 353 - 362, 英語[査読有り]
研究論文（学術雑誌）
When polyunsaturated fatty acids (PUFAs) in biomembrane are peroxidized, a great diversity of aldehydes is formed, and some of which are highly reactive. Thus they are thought to have biological impacts in stressed plants; however, the detailed mechanism of generation and biochemical effects are unknown. In this study, we show that chloroplasts are major organelles in which malondialdehyde (MDA) generated from peroxidized linolenic acid modifies proteins in heat-stressed plants. First, to clarify the biochemical process of MDA generation from PUFAs and its attachment to proteins, we carried out in vitro experiments using model proteins (BSA and Rubisco) and methylesters of C18 PUFAs that are major components of plant biomembrane. Protein modification was detected by Western blotting using monoclonal antibodies that recognize MDA binding to proteins. Results showed that peroxidation of linolenic acid methylester by reactive oxygen species was essential for protein modification by MDA, and the MDA modification was highly dependent on temperature, leading to a loss of Rubisco activity. When isolated spinach thylakoid membrane was peroxidized at 37 degrees C, oxygen-evolving complex 33 kDa protein (OEC33) was modified by MDA. These model experiments suggest that protein modification by MDA preferentially occurs under higher temperatures and oxidative conditions, thus we examined protein modification in heat-stressed plants. Spinach plants were heat-stressed at 40 degrees C under illumination, and modification of OEC33 protein by MDA was detected. In heat-stressed Arabidopsis plants, light-harvesting complex protein was modified by MDA under illumination. This modification was not observed in linolenic acid-deficient mutants (fad3fad7fad8 triple mutant), suggesting that linolenic acid is a major source of protein modification by MDA in heat-stressed plants. (C) 2008 Elsevier Masson SAS. All rights reserved.
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2008年08月, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46 (8-9), 786 - 793, 英語[査読有り]
研究論文（学術雑誌）
Parasitic plants cause devastating losses to crop yields in several parts of the world. The root parasites, Striga and Orobanche species, use chemical signalling molecules that are exuded by the roots of plants in extremely low concentrations, and that can induce germination of the seeds of these parasites, to detect the vicinity of a suitable host. The majority of the so far identified germination stimulants belong to the strigolactones. It was recently discovered that this class of compounds can also induce hyphal branching in the symbiotic arbuscular mycorrhizal fungi, a process involved in root colonisation. The elucidation of the structure of new strigolactones is hindered by their low abundance and instability. In the present paper, we have used existing knowledge on the structure of strigolactones and combined it with recently obtained insight in the biosynthetic origin of these signalling compounds. This enabled us to postulate structures for strigolactones that have been isolated but for which so far the structure has not been elucidated, but also to propose structures of strigolactones that may be discovered in the future. Considering the strongly increased importance of the strigolactones, we expect that more groups will look for these compounds and also in systems so far not exploited. This could lead to the discovery of new strigolactones for which we expect the present biogenetic considerations will facilitate identification and structure elucidation. (c) 2008 Elsevier Masson SAS. All rights reserved.
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2008年07月, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46 (7), 617 - 626, 英語[査読有り]
研究論文（学術雑誌）
A novel strigolactone sorgomol, germination stimulant for root parasitic plants Striga and Orobanche, was isolated and structure was elucidated. Sorgomol was more active on Striga than on Orobanche and may be the immediate precursor of sorgolactone in the biosynthetic pathway of strigolactones. (C) 2008 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2008年03月, TETRAHEDRON LETTERS, 49 (13), 2066 - 2068, 英語[査読有り]
研究論文（学術雑誌）
- 2008年03月, Weed Science, 56 (3), 356-363Molecular responses of Sorghum bicolor to Striga hermonthica parasitism
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研究論文（学術雑誌）
Lotus japonicus roots, cultured in a modified B5 medium, produced and secreted germination stimulants that induced Striga hermonthica seed germination. The germination-inducing activity was detected both in the roots and the culture filtrate. Following bioassay-guided purification procedures, an active compound was isolated from hexane extracts of the roots and the culture filtrate. Based on chromatographic behaviour on HPLC, and H-1 NMR, UV, NIS and CD spectroscopic analyses, the germination stimulant was identified as (+)-5-deoxystrigol. (C) 2007 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2008年01月, PHYTOCHEMISTRY, 69 (1), 212 - 217, 英語[査読有り]
研究論文（学術雑誌）
Strigolactones released from plant roots induce hyphal branching of symbiotic arbuscular mycorrhizal (AM) fungi and germination of root parasitic weeds, Striga and Orobanche spp. We already demonstrated that, in red clover plants (Trifolium pratense L.), a host for both AM fungi and the root holoparasitic plant Orobanche minor Sm., reduced supply of phosphorus (P) but not of other elements examined (N, K, Ca, Mg) in the culture medium significantly promoted the secretion of a strigolactone, orobanchol, by the roots of this plant. Here we show that in the. case of sorghum [Sorghum bicolor (L.) Moench], a host of both the root hemiparasitic plant Striga hermonthica and AM fungi, N deficiency as well as P deficiency markedly enhanced the secretion of a strigolactone, 5-deoxystrigol. The 5-deoxystrigol content in sorghum root tissues also increased under both N deficiency and P deficiency, comparable to the increase in the root exudates. These results suggest that strigolactones may be rapidly released after their production in the roots. Unlike the situation in the roots, neither N nor P deficiency affected the low content of 5-deoxystrigol in sorghum shoot tissues.
SPRINGER, 2007年12月, PLANTA, 227 (1), 125 - 132, 英語[査読有り]
研究論文（学術雑誌）
Strigolactones are germination stimulants for seeds of the root parasitic weeds, Striga and Orobanche spp. The imino analog of GR24 showed moderate germination stimulating activity against the seeds of S. hermonthica. The seed germination stimulating activity of some phenyliminoacetates and phenyliminoacetonitriles was also examined. The degree of activity of the phenyliminoacetate was less than that of the phenylacrylates. On the other hand, the degree of activity of the phenyliminoacetonitrile was comparable to that of the phenylacrylonitriles. Among the tested compounds, the 3-pyridyliminoacetonitrile showed higher activity against the seeds of O. crenata than GR24. These findings demonstrate that it is not always essential to have the Michael acceptor of the C-D ring junction moiety which has been proposed to react with nucleophilic species presented at the target site to enhance the activity.
TAYLOR & FRANCIS LTD, 2007年11月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 71 (11), 2781 - 2786, 英語[査読有り]
研究論文（学術雑誌）
N-15-Labeled dechlorodauricumine and dechloroacutumine were isolated from Menispermum dauricum roots cultured in a chloride-deficient medium, in which nitrogen-containing macro-components (KNO3)-N-14 and ((NH4)-N-14)(2)SO4 were replaced by (KNO3)-N-15 and ((NH4)-N-15)(2)SO4, respectively. These N-15-labeled substrates were supplied independently to the roots cultured in a chloride-enriched medium. LC-ESI-MS analysis of alkaloids extracted from the roots, harvested 5 and 10 days after administering the N-15-labeled substrates, revealed that the N-15 derived from dechlorodauricumine was much more effectively incorporated into chlorinated alkaloids than that derived from dechloroacutumine. These findings suggest that dechlorodauricumine is the principal precursor of the chlorinated alkaloids produced by M. dauricum roots. (c) 2006 Elsevier Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2007年02月, PHYTOCHEMISTRY, 68 (4), 493 - 498, 英語[査読有り]
研究論文（学術雑誌）
- 2007年, 精糖研究会誌, 55, 1-7, 日本語ショ糖を基質とした三酵素反応によるガラクチノールの合成
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研究論文（学術雑誌）
- 2007年, Sudan J. Agricultural Research, 10 (Special Issue), 121-126Molecular diversity of Striga hermonthica collected from different locations and host plant species
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研究論文（学術雑誌）
- 2007年, Sudan J.Agricultural Research, 10 (Special Issue)133-141,Marker assisted breeding of stay-green trait to enhance drought tolerance in sorghum
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研究論文（学術雑誌）
- 2007年, Sudan J. Agricultural Research, 10 (Special Issue), 127-132Isolation and expression analyses of genes up-regulated in sorghum roots infested by Striga hermonthica
[査読有り]
研究論文（学術雑誌）
- 2007年, Sudan J Agricultural Research, 10 (Special Issue), 93-100Effect of terpenoid biosynthesis inhibitors on strigol formation in Menispermum dauricum root culture
[査読有り]
研究論文（学術雑誌）
- 2006年12月, 精糖技術研究会誌, 54, 37-43, 日本語Development of methods for galactinol production using sucrose as a starting material,
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研究論文（学術雑誌）
- 11^
IUPAC農薬化学国際会議報告(談話室)一般社団法人植物化学調節学会, 2006年, 植物の生長調節, 41 (2), 173 - 174, 日本語 2005年11月, Phytochemistry, 66 (22), 2627-2631Dechlorodauricumine from cultured roots of Menipsermum dauricum, Phytochemistry[査読有り]
研究論文（学術雑誌）
Contribution of pre-anthesis assimilates and current photosynthesis to grain yield, and their relationships to drought resistance in wheat cultivars grown under different soil moistureWe investigated the relative importance of pre-anthesis assimilates stored in plant parts, mainly in the stem, and post-anthesis photosynthesis to drought resistance in wheat (Triticum aestivum L.) cultivars Hongwangmai (drought resistant) and Haruhikari (drought sensitive) subjected to two soil moisture regimes: irrigated and non-irrigated. In the irrigated treatment, soil moisture was maintained near field capacity throughout the growing season, while in the non-irrigated treatment water was withheld from 81 d after sowing until maturity. Drought stress reduced grain yield of Hongwangmai and Haruhikari by 41 and 60 %, respectively. Remobilization of pre-anthesis assimilates to the grain (remobilization) was reduced by drought in Hongwangmai but increased in Haruhikari. The contribution of pre-anthesis assimilates to the grain decreased under non-irrigated treatment in Hongwangmai. However, under water stress, Hongwangmai maintained a higher net photosynthetic rate in the flag leaf than Haruhikari. These results indicated that maintenance of post-anthesis photosynthetic rate was related to drought resistance in Hongwangmai rather than to remobilization under drought stress.
ACAD SCI CZECH REPUBLIC, INST EXPERIMENTAL BOTANY, 2004年, PHOTOSYNTHETICA, 42 (1), 99 - 104, 英語[査読有り]
研究論文（学術雑誌）
2004年, Sudan Journal of Agricultural Research, 4, 3-11Effect of foliar fertilization on sweet melon under saline conditions[査読有り]
研究論文（学術雑誌）
2004年, Vol. 4 - Biotechnology and Genetic Engineering (eds. J. N. Govil, P. A. Kumar & V. K. Singh) SCI TECH Pub., USA, 163-174,Biological activities of oxoisoaporphines isolated from Menispermum dauricum root cultures, In: Recent Research Progress in Medicinal Plants[査読有り]
研究論文（学術雑誌）
Ameliorating effect of calcium on primary root elongation of soybean under sodium stressThe present study was carried out to investigate the ameliorating effect of calcium on salt-induced inhibitory effects on primary root elongation. Following imbibition and germination, primary roots of soybean (Glycine max (L.) MERR.) cv. Dare, 40 +/- 0.4 mm in length, were exposed for 9 h to solutions of NaCl (10, 15, 25, 50, 100 mM) and 100 mM NaCl + 0.15, 1.0 or 5.0 mM CaCl(2). The presence of Na(+) significantly inhibited primary root elongation and Ca(2+) significantly alleviated this inhibition. The NaCl treatments significantly increased Na(+), decreased K(+) and Ca(2+) contents in elongation, middle and mature zones of primary roots compared with those of controls, respectively. Addition of Ca(2+) significantly decreased Na+, increased K+ and Ca(2+) contents in elongation, middle and mature zones of primary roots comparing to those deprived of Ca(2+), respectively. Thus, significantly higher K/Na and Ca/Na ratios were maintained in the former than the later roots. The decreasing gradient of Na+ accumulation from root tip towards hypocotyl indicated that Na+ exclusion ability was increasing with cell maturation. Results of Xray microanalysis showed that the epidermis cells accumulated largest fraction of Ca(2+) among the three ions, compared with those of cortex and stele cells. The Ca/Na and K/Na ratios were higher in epidermis than in cortex. These results suggest that Ca(2+) interacted with Na(+) mostly in the epidermis. As a result, the amount of Na(+) entered the primary roots was reduced and hence the adverse effects of Na(+) on primary root elongation were ameliorated.
VERSITA, 2004年, BIOLOGIA, 59, 129 - 135, 英語[査読有り]
研究論文（学術雑誌）
Effect of drought on ear and flag leaf photosynthesis of two wheat cultivars differing in drought resistanceWe investigated net photosynthetic rate (P-N) of ear and two uppermost (flag and penultimate) leaves of wheat cultivars Hongmangmai (drought resistant) and Haruhikari (drought sensitive) during post-anthesis under irrigated and non-irrigated field conditions. The P-N of ear and flag leaf were significantly higher and less affected by drought in Hongmangmai than in Haruhikari. The rate of reduction in stomatal conductance (g(s)) was similar for the two cultivars, but intercellular CO2 concentration (C-i) in the flag leaf of Hongmangmai was lower than that of Haruhikari in non-irrigated treatment. No differences were observed in leaf water potential (psi(1)) and osmotic adjustment of the flag leaf of the cultivars. These results imply that differences in photosynthetic inhibition on the flag leaf at low leaf T, between the cultivars were primarily due to non-stomatal effects. Hence the main physiological factor associated with yield stability of Hongmangmai under drought stress may be attributed to the capacity for chloroplast activity in the flag leaf, which apparently allows sustained P-N of flag leaf during grain filling under drought stress. The higher P-N of ear in Hongmangmai under drought could also be related to its drought resistance.
ACAD SCI CZECH REPUBLIC, INST EXPERIMENTAL BOTANY, 2004年, PHOTOSYNTHETICA, 42 (4), 559 - 565, 英語[査読有り]
研究論文（学術雑誌）
2003年11月, Acta Bot.- Occident. Sin, 23 (11), 1861-1870Compensation effects of planting density and fertilization on grain yield, water use efficiency and seeds nutrient contents of dry land spring wheat,[査読有り]
研究論文（学術雑誌）
Germination strategy of Striga hermonthica involves regulation of ethylene biosynthesisEthylene involvement in germination of Striga hermonthica (Del.) Benth., an important root parasitic weed on poaceous crops, was investigated at the physiological and molecular levels. Seeds, conditioned at 30degreesC for 14 days, were treated with ethylene, ethephon or 1-aminocyclopropane-1-carboxylic acid (ACC). Ethylene consistently induced low germination. Ethephon and ACC effectively stimulated germination at concentrations of 0.01 and 1 mM, respectively. In contrast to ethylene, both ethephon and ACC acted in a concentration-dependent manner. Germination induced by the synthetic strigolactone GR24 was inhibited by aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene. ACC reversed the inhibition caused by AVG. When seeds were treated with GR24 in sealed vials, ethylene concentration in headspace gas increased prior to the onset of germination. Total RNA extracted from germinating seeds 12 h after GR24 treatment was used for PCR-based amplification of cDNA fragments encoding the ACC synthase- and oxidase-active site domains. Two distinct cDNA fragments encoding ACC synthase (SHACS1 and SHACS2) and one encoding ACC oxidase (SHACO1) were cloned and sequenced. Southern analysis suggested that each of the cloned genes was present as a single copy in the genome of S. hermonthica. Northern analyses showed that SHACS1 exhibited a temporal change in expression peaking at 10 h after GR24 treatment, which coincided with a steady increase in ethylene concentration. SHACS2 was expressed at a low level with a similar trend. SHACO1 exhibited a temporal change in expression peaking at 15 days during conditioning, when seed response to GR24 was maximal. In summary, expression of ACC synthase and ACC oxidase genes was found to be responsive to a germination stimulant and to conditioning, respectively. The implications of these findings with respect to germination of S. hermonthica under field conditions are discussed.
WILEY, 2003年09月, PHYSIOLOGIA PLANTARUM, 119 (1), 137 - 145, 英語[査読有り]
研究論文（学術雑誌）
Silicon-induced changes in viscoelastic properties of sorghum root cell wallsSilicon is deposited in the endodermal tissue in sorghum (Sorghum bicolor L. Moench) roots. Its deposition is thought to protect vascular tissues in the stele against invasion by parasites and drying soil via hardening of endodermal cells. We studied the silicon-induced changes in mechanical properties of cell walls to clarify the role of silicon in sorghum root. Sorghum seedlings were grown in nutrient solution with or without silicon. The mechanical properties of cell walls were measured in three separated root zones: basal, apical and subapical. Silicon treatment decreased cell-wall extensibility in the basal zone of isolated stele tissues covered by endodermal inner tangential walls. The silicon-induced hardening of cell walls was also measured with increases in elastic moduli (E) and viscosity coefficients (eta). These results provided new evidence that silicon deposition might protect the stele as a mechanical barrier by hardening the cell walls of stele and endodermal tissues. In contrast to the basal zone, silicon treatment increased cell-wall extensibility in the apical and subapical zones with concomitant decrease in E and eta. Simultaneously, silicon promoted root elongation. When root elongation is promoted by silicon, one of the causal factors maybe the silicon-enhanced extensibility of cell walls in the growing zone.
OXFORD UNIV PRESS, 2003年07月, PLANT AND CELL PHYSIOLOGY, 44 (7), 743 - 749, 英語[査読有り]
研究論文（学術雑誌）
(+)-Strigol, a witchweed seed germination stimulant, from Menispermum dauricum root culture(+)-Strigol was isolated from Menispermum dauricum root culture filtrate. Its identity was confirmed by HPLC, H-1 NMR, UV and MS, and on the basis of its CD spectrum. This is the first report on isolation of strigolactone from aseptic plant culture. (C) 2003 Elsevier Science Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 2003年04月, PHYTOCHEMISTRY, 62 (7), 1115 - 1119, 英語[査読有り]
研究論文（学術雑誌）
A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using highperformance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/muL of strigol and 0.5 pg/muL of sorgolactone could be quantified, whereas 1 pg/muL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively.
AMER CHEMICAL SOC, 2003年02月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 51 (5), 1162 - 1168, 英語[査読有り]
研究論文（学術雑誌）
一般社団法人植物化学調節学会, 2003年, 植物の生長調節, 38 (1), 146 - 146, 英語一般社団法人植物化学調節学会, 2003年, 植物の生長調節, 38 (1), 145 - 145, 日本語2003年, ChineseJournal of Eco-Agriculture, 11(1)pp.1-5, 英語Isolation of a germination stimulant for Striga hermonthica (Del) Benth from root culture of Menispermum dauricum DC[査読有り]
研究論文（学術雑誌）
Effect of sowing dates on the incidence of bacterial blight and yield of cottonBacterial blight (BL) is a common disease affecting the growth, development and yield of cotton in Sudan. Field trials were conducted for two seasons to determine the influence of four sowing dates (July 15 and 31, August 15 and September 1), representing heavy and low rainfall periods, on disease incidence and growth of two cotton cultivars (Barakat 90 and Barac (67)B. For the two cultivars, the incidence of BL decreased with delayed sowing. However, the number of cotton bolls was significantly decreased and seed cotton yield was reduced by an average of 12, 20 and 33% in the last three sowing dates compared with sowing in mid July. Fiber bundle strength and micronaire readings were also markedly reduced by delayed sowing while fiber length was unaffected. Bacterial blight reduced chlorophyll concentration (on dry leaf weight basis) by 80, 52, and 19% in severe, moderate and low disease infections, respectively. However, chlorophyll concentration of uninfected leaves was increased by delayed sowing. When sowing was delayed to mid August and early September, the total soluble carbohydrate of uninfected leaves of Barakat 90 was significantly reduced compared with mid and late July sowing. Our results indicate that sowing date is a more important factor that determines cotton yield and yield components than disease incidence and severity.
URBAN & FISCHER VERLAG, 2003年, BASIC AND APPLIED ECOLOGY, 4 (5), 433 - 440, 英語[査読有り]
研究論文（学術雑誌）
A field study was conducted to evaluate the drought tolerance of three sorghum [Sorghum bicolor (L.) Moench] cultivars, Gadambalia, Arous elRimal and Tabat, and quantify the physiological bases for differences in their drought tolerance. Water stress reduced shoot dry mass of Gadambalia, Arous elRimal and Tabat by 43, 46 and 58 %, respectively. The respective reduction in leaf area of the three cultivars was 28, 54 and 63 %. The reduction in net photosynthetic rate, stomatal conductance and transpiration rate due to water stress was lowest in Gadambalia and highest in Tabat. The leaf water potentials and relative water contents of Gadambalia under wet and dry treatments were similar, while those of Tabat were significantly reduced by water stress. The lowest and highest liquid water flow conductance was displayed by Tabat and Gadambalia, respectively. Drought tolerance in Gadambalia is associated with its smaller leaf area, higher liquid water flow conductance, and ability to maintain high leaf water potential, relative water content, stomatal conductance, transpiration rate and photosynthetic rate under drought stress.
KLUWER ACADEMIC PUBL, 2003年, BIOLOGIA PLANTARUM, 46 (4), 583 - 587, 英語[査読有り]
研究論文（学術雑誌）
Trichothecene inhibitors of Striga hermonthica germination produced by Fusarium solaniMetabolites of the fungus Fusarium solani (Sud 96) inhibited Striga hermonthica germination induced by the germination stimulant GR24 The active principles were identified as trichothecenes acuminatin, neosolaniol, 8-acetylneosolaniol, and tetraacetoxy T-2 tetraol (neosolaniol diacetate) on the basis of their chromatographic behavior and nuclear magnetic resonance and mass spectra Inhibitory activity of the four trichothecenes against Striga germination increased with acetylation of the hydroxyl moieties The most abundant inhibitor produced by the fungus, 8-acerylneo-solaniol, completely inhibited Striga germination at 24 muM The fungal toxin did not affect the germination of sorghum, a host crop, but retarded root and shoot elongation of the seedlings by 60 and 30%, respectively, at the same concentration.
WEED SCI SOC AMER, 2002年09月, WEED SCIENCE, 50 (5), 658 - 661, 英語[査読有り]
研究論文（学術雑誌）
Silicification in sorghum (Sorghum bicolor) cultivars with different drought toleranceSorghum belongs to a group of economically important, silicon accumulating plants. X-ray microanalysis coupled with environmental scanning electron microscopy (ESEM) of fresh root endodermal and leaf epidermal samples confirms histological and cultivar specificity of silicification. In sorghum roots, silicon is accumulated mostly in endodermal cells. Specialized silica aggregates are formed predominantly in a single row in the form of wall outgrowths on the inner tangential endodermal walls. The density of silica aggregates per square mm of inner tangential endodermal cell wall is around 2700 and there is no significant difference in the cultivars with different content of silicon in roots. In the leaf epidermis, silicon deposits were present in the outer walls of all cells, with the highest concentration in specialized idioblasts termed 'silica cells'. These cells are dumb-bell shaped in sorghum. In both the root endodermis and leaf epidermis, silicification was higher in a drought tolerant cultivar Gadambalia compared with drought sensitive cultivar Tabat. Silicon content per dry mass was higher in leaves than in roots in both cultivars. The values for cv. Gadambalia in roots and leaves are 3.5 and 4.1% Si, respectively, and for cv. Tabat 2.2 and 3.3%. However, based on X-ray microanalysis the amount of Si deposited in endodermal cell walls in drought tolerant cultivar (unlike the drought susceptible cultivar) is higher than that deposited in the leaf epidermis. The high root endodermal silicification might be related to a higher drought resistance.
BLACKWELL MUNKSGAARD, 2002年05月, PHYSIOLOGIA PLANTARUM, 115 (1), 87 - 92, 英語[査読有り]
研究論文（学術雑誌）
Bis(8-quinolinolato-N,O)platinum(II), [Pt(C9H6NO)(2)], (I), has a centrosymmetric planar structure with trans coordination. The molecules form an inclined pi stack, with an interplanar spacing of 3.400 (6) Angstrom. 8-Hydroxyquinolinium dichloro(8-quinolinolato-N,O)platinate(II) tetrahydrate, (C9H8NO)[PtCl2 (C9H6NO)].4H(2)O, (II), is soluble in water and is regarded as the synthetic intermediate of the insoluble neutral compound (I). The uncoordinated 8-hydroxyquinolinium cations and the monoquinolinolate complexes form an alternating pi stack. The origins of fluorescence and phosphorescence in (II) are assigned to the 8-hydroxyquinolinium cation and the monoquinolinolate-Pt complex, respectively.
BLACKWELL MUNKSGAARD, 2002年03月, ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE COMMUNICATIONS, 58 (3), m147 - m149, 英語[査読有り]
研究論文（学術雑誌）
Experiments were conducted in an environmentally controlled growth chamber to evaluate the role of the root system in the salt tolerance of two soybean (Glycine max (L.) Merr.) cultivars, Tachiyutaka and Dare, that differ in salt tolerance. Young plants were submitted to 0 (control), 40, 80, and 120 mM NaCl treatments for 3, 23, and 30 days in three experiments, respectively. The most significant differences between the two cultivars were obtained in 40 mM NaCl. Under this condition, compared with salt sensitive cv. Tachiyutaka, tolerant cv. Dare showed a higher relative shoot and root growth (Dare: 70 and 63%, Tachiyutaka: 42 and 42%, respectively), water extraction ability (Dare: 0.18 g cm(-2) d(-1) for transpiration and 0.98 g m(-1) d(-1) for water uptake, Tachiyutaka: 0.13 g cm(-2) d(-1) and 0.81 g m(-1) d(-1) respectively), root pressure (Dare: 5.68 mg g(-1) for root exudate, Tachiyutaka: 0.32 mg g(-1)), better root osmotic adjustment (Dare: -0.61 MPa for osmotic potential, Tachiyutaka: -0.59 MPa), and less sodium (Na+) accumulation in plant tissue (Dare: 0.1, 0.4, and 9.2 mg g(-1) in leaf, stem, and root, respectively, Tachiyutaka: 1.4, 3.5, and 11.6 mg g(-1)). The difference in salt tolerance between the two cultivars is attributed to the root system. The salt tolerance of cv. Dare is associated with high water uptake, and Na+ and chloride (Cl-) exclusion of the roots.
MARCEL DEKKER INC, 2002年, JOURNAL OF PLANT NUTRITION, 25 (3), 407 - 423, 英語[査読有り]
研究論文（学術雑誌）
一般社団法人植物化学調節学会, 2002年, 植物の生長調節, 37 (2), 222 - 222, 日本語The effect of mineral nutrition status of mother plant on the concentrations of amino and fatty acids and the emergence quality of hybrid sweet pepper seeds (cv. 'Hazera' 1195) was studied in two hydroponics experiments. Three concentrations of potassium (K) (0.5, 2.5 and 5.0 mM) combined with NH4-N to NO3-N ratios (mM/mM: 0/6.0, 0.9/5.1, 1.8/4.2 and 3.0/3.0) were conducted during the seasons from spring to summer and from autumn to winter, respectively. Seeds developed in the summer showed lower emergence percentage than those developed in the winter. When the mother plants were supplied by the same nitrogen (N) form and K concentration, the summer seeds contained significant higher K and chloride (Cl), total free amino acids, oleic acids, stearic acid, and palmitoleic acid, but lower phosphorus (P), calcium (Ca), magnesium (Mg), proline and linoleic acid than the winter seeds. No difference was found in the concentrations of N, sulfur (S), palmitic acid and total fatty acids of seeds between the two seasons. Replacing of 3 mM NO3-N (50% of total N) by NH4-N or the reduction of K concentration in the solution from 2.5 mM to 0.5 mM decreased proline, histidine, total amino acids, linoleic acid, and increased the ratio of oleic acid to stearic acid in the summer seeds. The correlation and stepwise regression analysis suggested that the emergence percentage of seeds developed in the summer was positively correlated to the proline concentration, but negatively correlated to the concentrations of Cl and K and the ratio of oleic to stearic acid.
2002年, Journal of Plant Nutrition, 25 (8), 1645 - 1665, 英語[査読有り]
研究論文（学術雑誌）
Effects of salinity and relative humidity on two melon cultivars differing in salt toleranceThe effects of increasing relative humidity on the growth and salt tolerance of two melon (Cucumis melo L.) cultivars, Revigal C-8 (salt sensitive) and Galia (salt tolerant) was investigated. One month after germination, the plants were exposed for 15 d to 0 (control) and 80 mM NaCl, under relative humidity (RH), 30 and 70 %. The growth of the whole plant, leaf, stem and root of cv. Revigal C-8 was increased with increasing RE. On the other hand, cv. Galia showed an increase in root growth with increasing RH only under the NaCl treatment. Under salinity, most of the Na(+) was withheld in the stems. An increase in RE in the NaCl treatment significantly decreased Na(+) and Cl(-) concentrations in leaves of cv. Revigal C-8, while it had no effect on their concentrations in cv. Galia. In both cultivars, increasing RH under NaCl condition significantly decreased water contents in leaves and stems, and increased osmotic potential in roots. The amount of the root exudate of cv. Galia was significantly decreased with increasing RH, while it was not affected in cv. Revigal C-8. Under the NaCl treatment, cv. Galia had significantly higher leaf osmotic potential than cv. Revigal C-8 at both relative humidities and higher amount of root exudate at 30 % RH.
SPRINGER, 2002年, BIOLOGIA PLANTARUM, 45 (3), 409 - 415, 英語[査読有り]
研究論文（学術雑誌）
An isolate of Fusarium solani (Sud 96) obtained from infected Strika plants in Sudan and six other isolates from Japan were evaluated for their effects on Striga germination. Among all the isolates, only the one from Sudan demonstrated high inhibitory activity. Aqueous and organic solvent culture extracts, as well as fungus suspension, when mixed with GR24, a synthetic analog of the natural germination Stimulant strigol, inhibited germination of conditioned Striga seeds. Fusarium solani (Sud 96) filtrates, from cultures grown on autoclaved rice, sorghum grains, and potato dextrose agar (PDA), were more effective in reducing Striga germination than those from cultures grown on wheat straw. A significant difference between rice compared to sorghum and PDA cultures only occurred at high dilutions (40-fold). Complete inhibition of germination occurred when F solani (Sud 96) culture filtrates and GR24 were applied simultaneously. Filtrate treatments made 2, 4 and 6 h subsequent to treatment with GR24 were less inhibitory. Filtrate treatments applied 8 h or more following GR24 had negligible effects on germination. Chromatographic separation on a silica gel column indicated the presence of several compounds with high inhibitory activity.
WEED SCI SOC AMER, 2001年05月, WEED SCIENCE, 49 (3), 354 - 358, 英語研究論文（学術雑誌）
Feeding experiments using Cl-36 showed that Melzispermum,dauricum root culture produces four alkaloids containing chlorine. They included the novel alkaloids dauricumine and dauricumidine as well as the known alkaloids acutumine and acutumidine. The structures of novel alkaloids were established by spectroscopic, crystallographic, and chemical methods. These four alkaloids were labeled with Cl-36, isolated, and fed independently to root cultures. Mutual conversion between acutumine and acutumidine, and between dauricumine and dauricumidine by N-methylation and N-demethylation, was demonstrated. Moreover, dauricumine was converted to acutumine and acutumidine. Epimerization of acutumidine to dauricumidine or vice versa was not observed. These results suggest that dauricumine is the first chlorinated alkaloid formed in cultured M. dauricum roots. Skewed distribution of radioactivity derived from labeled dauricumine is proof that epimerization at C-l proceeds at a lower rate than N-demethylation.
AMER CHEMICAL SOC, 2001年05月, JOURNAL OF ORGANIC CHEMISTRY, 66 (10), 3299 - 3302, 英語[査読有り]
研究論文（学術雑誌）
A new alkaloid, acutudaurin, was isolated from cultured roots of Menispermum dauricum, a rich source of the chlorine-containing alkaloid acutumine and its dechlorinated analogue dechloroacutumine. The structure of acutudaurin was determined to be 2',3'-dihydro-5-hydroxy-4,6',7'-trimethoxy-1'-methylspiro[3-cyclohexene-1,10'-[3a,7a] propano[1H]indole]-2.5'(4'H)-dione by spectroscopic methods.
GEORG THIEME VERLAG KG, 2001年03月, PLANTA MEDICA, 67 (2), 194 - 195, 英語[査読有り]
研究論文（学術雑誌）
Different effect of humidity on growth and salt tolerance of two soybean cultivarsTwo soybean (Glycine max (L.) Merr) cultivars, Tachiyutaka and Dare, were grown in pots at 30 and 70% relative humidity (RH) and treated with 0 (control), 40 (moderate), 80 and 120 (severe) mM NaCl for 3 weeks. Increasing RH enhanced growth of salt sensitive cultivar, Tachiyutaka, but had no effect on salt tolerant cultivar. Dare, under control and moderate saline conditions. Both cultivars benefited from elevated humidity under severe saline conditions. Cultivar Tachiyutaka had poorer ability for controlling translocation of Na(+) to the leaves, lower Na(+) exclusion ability in the roots, and lower root activity under NaCl treatment, compared with cv. Dare. The increased growth of cv. Tachiyutaka at high RH was consistent with decreased Na(+) accumulation in the leaves, increased stomatal conductance and root activity, while the unchanged growth of cv. Dare was consistent with similar Na(+) accumulation in the leaves, and the decreased root activity.
SPRINGER, 2001年, BIOLOGIA PLANTARUM, 44 (3), 405 - 410, 英語[査読有り]
研究論文（学術雑誌）
Conditioning period, CO2 and GR24 influence ethylene biosynthesis and germination of Striga hermonthicaGermination of witchweed (Striga hermonthica [Del,] Benth), an important root parasite on poaceous crops, requires pretreatment 'conditioning' in a warm moist environment and a subsequent exposure to a stimulant. The roles of conditioning period, CO2 and a strigol analogue (GR24) in ethylene biosynthesis and germination of the parasite were investigated, Conditioning increased the seeds' capacity to oxidize exogenous 1-aminocyclopropane-1-carboxylic acid (ACC), Exogenous CO2 increased the seeds capacity to oxidize ACC by 3- to 9-fold, A combination of GR24 and ACC increased ethylene production by more than 3-foId in comparison with the rates obtained using these compounds separately. Aminoethoxyvinylglycine (AVG) completely inhibited ethylene induction by GR24, but not by ACC. A GR24 treatment, made subsequent to conditioning in GR24, did not induce ethylene. However, seeds conditioned in GR24 and then given 1 mM ACC produced 293 nl l(-1) ethylene, ACC oxidase (ACCO) activity in crude extracts was increased by conditioning and CO2. The enzyme displayed an absolute requirement for ascorbate. Absence of exogenous Fe2+ reduced enzyme activity only by 14%, GR24 applied during conditioning reduced germination in response to a subsequent GR24 treatment, ACC was, invariably, less effective in inducing S, hermonthica germination than GR24 even at concentrations which induce more ethylene than concurrent GR24 treatments. The results are consistent with a model in which conditioning removes a restriction on the ethylene biosynthetic pathway in S. hermonthica seeds. GR24 modulates the key enzymes in ethylene biosynthesis. The stimulant suppresses ethylene biosynthesis in unconditioned seeds and promotes it in conditioned ones. Germination of S, hermonthica results from the joint action of GR24 and the ethylene it induces.
MUNKSGAARD INT PUBL LTD, 2000年05月, PHYSIOLOGIA PLANTARUM, 109 (1), 75 - 80, 英語[査読有り]
研究論文（学術雑誌）
The growth of sesame (Sesamum indicum L.) was studied at three root temperature regimes (25/25, 20/10 and 15/15°C day/night) factorially combined with three NO3-: NH4+ ratios (mM ratios, 10:0, 8:2, or 6:4), as a source of nitrogen (N), in the irrigation solution. The air temperature was kept constant at 30°C. Transpiration, nutrient composition, and level of root-born cytokinins and gibberellins in the xylem exudate were monitored. The two low root temperature regimes, 15/15 and 20/10°C, restricted the growth of sesame, reduced transpiration and increased the accumulation of soluble carbohydrates in the shoot and in the roots compared to the 25/25°C regime. The NO3+:NH4+ ratios had no effect on growth. Nutrient contents in the shoot at low root temperatures, particularly K+, NO3-, and H2PO4- were decreased markedly, but Na+ increased relative to it's content in the 25/25°C regime. Increasing NH4+ proportion in the irrigation solution raised total N concentration in the plant tissues at all root temperatures. The amounts of cytokinins and gibberellins in the xylem exudate decreased at the low root temperature regimes relative to the 25/25°C regime. Low root temperature reduced xylem transport of nutrients and root born-phytohormones, most probably because of reduced water flow through the plant relative to the 25/25°C regime.
Marcel Dekker Inc., 2000年, Journal of Plant Nutrition, 23 (1), 123 - 140, 英語[査読有り]
研究論文（学術雑誌）
The growth of crown and lateral roots emerged from the excised phytomers of pearl millet (Pennisetum typhoideum Rich.), barnyard millet (Echinochloa frumentacea Link.) and maize (Zea mays L.) was studied under normal and low osmotic potential conditions. The plants were grown in two solutions with osmotic potentials of -0.02 and -0.54 MPa for 6 days. The relative growth rate of the roots in total length (RGR(L)) , was not affected by osmotic stress in pearl and barnyard millets, but that in stressed maize was reduced to 64% of the control. Similarly, the relative growth rate of the roots in dry weight (RGRW), was not affected by osmotic stress in pearl and barnyard millets, but significantly reduced in maize. Osmotic stress increased the specific root length of the lateral roots in pearl and barnyard millets, but did not affect that of maize. The photosynthetic rate (Pr) in the two millets was decreased to ca. 60% of the control by the stress, and that in maize was reduced to 21%. Under the stress, Pr in the three species was limited mainly by low stomatal conductance, but no clear relationship was found between Pr and osmotic adjustment of the leaf. The relative water content of the leaf was lower in maize than in the two millets. The resistance to water flow through the phytomer (R) was significantly increased by osmotic stress in maize, but not significantly in the two millets. The mean root length (R̄L̄) was decreased by the stress in maize, but not in the two millets. The maintenance of RGR(L) in the two millets was associated with sustained R, R̄L̄ and also with the maintenance of Pr and allocation of assimilates to roots.
Crop Science Society of Japan, 2000年, Plant Production Science, 3 (1), 55 - 60, 英語[査読有り]
研究論文（学術雑誌）
Two oxoisoaporphine alkaloids, 2,3-dihydrodauriporphine and tyraminoporphine, together with the known alkaloid dauriporphine, were isolated from Menispermum dauricum roots cultured in a medium containing ketoconazole, a cytochrome P-450 inhibitor. Structures of the alkaloids were established by spectroscopic, crystallographic and chemical methods. (C) 1999 Elsevier Science Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 1999年12月, PHYTOCHEMISTRY, 52 (8), 1431 - 1435, 英語[査読有り]
研究論文（学術雑誌）
The effects of chloride ion on the production of acutumine and dechloroacutumine, by Menispermum dauricum root culture, were studied. The chloride ion content in the medium plays a key role in the production of both alkaloids. A low chloride medium promoted production of dechloroacutumine and suppressed that of acutumine. Production of the two alkaloids during the 60 day culture period was closely associated with root biomass. Both alkaloids accumulated in the roots and a relatively small proportion was exuded into the medium. The intact plant produced very low amounts of both alkaloids. On the average, cultured roots contained 22- and 75-fold more acutumine and dechloroacutumine, respectively, than intact plants.
1999年03月10日, Phytochemistry, 50 (5), 775 - 779, 英語[査読有り]
研究論文（学術雑誌）
Several mechanisms have been proposed to explain the high tolerance of sorghum [Sorghum bicolor (L.) Moench] to drought. This paper reports a field study on the effects of soil moisture stress on the rooting habits, transpiration rate, and xylem anatomy of two sorghum cultivars, Tabat (drought susceptible) and Gadambalia (drought tolerant). Two levels of water stress, -0.02 MPa (wet) and -0.75 MPa (dry), were applied. Tabat had a higher root length density (RLD), higher late metaxylem (LMX) vessels per nodal root, higher leaf area, and higher transpiration rate than Gadambalia. In Tabat, soil moisture stress reduced RLD by 30%, nodal roots by 31%, number of LMX vessels in the root by 42%, leaf area by 13%, and transpiration rate by 11%. In Gadambalia soil moisture stress did not affect RLD at depths ≥0.2 m, number of nodal roots, or number of LMX vessels per nodal root. However, leaf area and transpiration rate were reduced by 3 and 11%, respectively. Under dry conditions, Gadambalia displayed a higher water extraction efficiency than Tabat throughout the profile (0-0.9 m). In Gadambalia, unlike Tabat, the stem was highly sclerified. A 1- to 3-cell-thick layer of schlerenchyana was observed beneath the epidermis. The peripheral vascular bundles were surrounded with a 3- to 6-cell-thick schlerenchyma sheath. However, in roots anatomical differences were less prominent. Drought tolerance in Gadambalia is associated with higher water extraction efficiency, fewer nodal roots per plant, fewer LMX vessels per nodal root, a smaller leaf area, and a well developed sclerenchyma.
Crop Science Society of America, 1999年, Crop Science, 39 (1), 168 - 173, 英語[査読有り]
研究論文（学術雑誌）
The biosynthetic relationship between acutumine 1 and dechloroacutumine 2 was studied using 13C-labeled tyrosine and 3H-labeled 2 as tracers. 13C-NMR spectra of 13C-labeled 1 and 2 showed that the alkaloids, each composed of two molecules of tyrosine, are derived from the same biosynthetic pathway. Feeding Menispermum dauricum (Menispermaceae) roots, cultured in a chloride-enriched medium, with 3H-labeled 2 demonstrated that 1 is the only alkaloid metabolite of 2. Conversion (5%) of the exogenously applied 2, taken up by the roots, into 1 showed that 2 is the precursor of 1. Incomplete conversion of 2 into 1 suggests accumulation of the exogenously applied 2 in cell organelles and/or compartmentation of the enzymes involved in the biosynthesis of 1. © 1999, Taylor & Francis Group, LLC. All rights reserved.
1999年01月01日, Bioscience, Biotechnology and Biochemistry, 63 (3), 515 - 518, 英語[査読有り]
研究論文（学術雑誌）
1998年12月, Plant Prod. Sci., 1 (4), 248 - 253, 英語[査読有り]
A novel alkaloid, dechloroacutumine, was isolated from Menispermum dauricum roots, a rich source of the chlorine-containing alkaloid acutumine, cultured in chlorine-deficient medium. Its structure was elucidated by spectral and crystallographic analysis. (C) 1998 Elsevier Science Ltd. All rights reserved.
PERGAMON-ELSEVIER SCIENCE LTD, 1998年11月, PHYTOCHEMISTRY, 49 (5), 1293 - 1297, 英語[査読有り]
研究論文（学術雑誌）
Effect of jasmonates and related compounds on seed germination of Orobanche minor Smith and Striga hermonthica (Del.) BenthJasmonates and related compounds were found to elicit the seed germination of the important root parasites, clover broomrape (Orobanche minor Smith) and witchweed [Striga hermonthica (Del.) Benth]. The stimulation of seed germination by the esters was more effective than by the corresponding free acids, and methyl jasmonate (MJA) was the most active stimulant among the compounds tested.
TAYLOR & FRANCIS LTD, 1998年07月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62 (7), 1448 - 1450, 英語[査読有り]
研究論文（学術雑誌）
Effect of medium composition on production of Striga hermonthica (Del.) Benth germination stimulant(s) by Menispermum dauricum (DC.) root culturesGermination of Striga hermonthica (Del.) Benth, a noxious parasitic weed on cereals, requires an exogenous stimulant produced by the roots of host and some nonhost plant species. Root cultures of Menispermum dauricum (DC.), a nonhost broad-leaved herbaceous plant, produced a group of substances that induce the parasite germination. This paper reports the establishment of a high-stimulant-producing M. dauricum root culture by manipulation of culture composition. A modified B5 medium (MB5) containing 35.7 mM nitrogen at a NO3-/NH4+ ratio of 1:42, 0.1 mM Fe2+, 1.0 mM Ca2+, 0.55 mM inorganic phosphorus, 0.28 mM inositol, 4.1 mu M nicotinic acid, 3.7 mu M I pyridoxine hydrochloride, 14.8 mu M thiamin hydrochloride, 1 mu M 1-naphthaleneacetic acid (NAA), and 4% sucrose sustained root growth for a longer period and increased root biomass by >30% and stimulant production by 5-fold, in comparison to the standard B5 medium supplemented with 3% sucrose and 1 mu M NAA.
AMER CHEMICAL SOC, 1998年04月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 46 (4), 1587 - 1592, 英語[査読有り]
研究論文（学術雑誌）
Several fungal metabolites were examined for their effects on germination of the root parasitic weeds witchweed, Striga hermonthica (Del.) Benth, and clover broomrape, Orobanche minor Smith. Among these metabolites cotylenins (CNs) and fusicoccins (FCs) at concentrations as low as 10(-5) M induced high seed germination (>50%) of both parasites. Inhibitors of ethylene biosynthesis [2-(2-aminoethoxyvinyl)glycine (AVG)] and action [silver thiosulfate (STS)] reduced CN- and FC-induced Striga germination but not that of Orobanche. This suggests that induction of Striga germination by CNs and FCs, as is the case with the true natural stimulant "strigol", requires both ethylene biosynthesis and action, while that of Orobanche does not.
AMER CHEMICAL SOC, 1998年04月, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 46 (4), 1583 - 1586, 英語[査読有り]
研究論文（学術雑誌）
A new non-destructive method for the measurement of root elongation in soil was developed using acoustic emission (AE) sensors. Collisions between soil particles caused by the growth of the root tip generated sound pulses that were detected by the AE sensors. In a simulation experiment, using a stainless steel rod (d= 1.3 mm), AE counts increased as the rod approached the sensor. The relative AE count (Ri) calculated from the values obtained from two sensors, vertically placed 15 mm apart, was only slightly influenced by the rod penetration rate and soil conditions, and linearly correlated with the distance between the rod tip and the center of the upper AE sensor. In the experiments using a maize primary root, Ri (Y) was significantly correlated with the distance (X) between the root tip and the center of the upper AE sensor as in the simulation experiment. Accordingly the root tip position could be estimated using the equation Y=0.994-0.0640X (r=0.883**). Calculated values for root elongation were comparable to those obtained from actual measurements.
Crop Science Society of Japan, 1998年03月, Plant Production Science, 1 (1), 25 - 29, 英語[査読有り]
研究論文（学術雑誌）
The naturally occurring sesquiterpene sorgolactone (2) belongs to the class of "strigolactones", which are highly potent germination stimulants for seeds of the parasitic weeds Striga and Orobanche. The aim of the present work was to synthesize all eight stereoisomers of sorgolactone and to evaluate their activities in the stimulation of germination of S. hermontica and O. crenata. Two racemic diastereomers of the ABC part of sorgolactone, rac.10a and rac.10b respectively, were prepared and coupled with homochiral latent D-ring synthons 12 and ent.12. In this manner, four mixtures of two separable (protected) sorgolactone diastereomers were obtained. Deprotection gave all eight target compounds as single isomers. Bioassays revealed that only those isomers possessing the same stereochemistry as natural sorgolactone at two adjacent chiral centers exhibit high biological activities.
American Chemical Society, 1998年02月20日, Journal of Organic Chemistry, 63 (4), 1259 - 1267, 英語[査読有り]
研究論文（学術雑誌）
Oilseed rape (Brassica napus L.) response to root temperature regimes (20/20, 16/8 and 12/12°C day/night) at constant 20°C air temperature was studied. At each regime, three NO3 -:NH4 + ratios (10:0, 8:2, or 6:4), at constant 10 mM N, in the irrigation solution were tested. Plant growth, transpiration, ionic composition and level of cytokinins and gibberellins in the xylem exudate were monitored. The two low root temperature regimes, 12/12 and 16/8°C, reduced rape shoot growth by 28 and 22%, and increased the accumulation of soluble carbohydrates by 42 and 26% in the roots, respectively, as compared to the 20/20°C regime. Low root temperatures reduced plants transpiration. The NO3 -:NH4 + ratios had no effect on rape growth. At low root temperatures NO3-contents increased in the shoot and decreased in the roots. The sum of cations and that of anions at 12/12 and 16/8°C root temperatures decreased significantly as compared to 20/20°C. The presence of NH4 + in the irrigation solution decreased the concentrations of Ca2+ and Mg2+ in the shoots and roots and increased that of Cl- in the shoots and of H2PO4 - in the roots at all root temperatures. Cytokinins and gibberellins contents in the xylem exudate decreased at the low root temperature regimes. Low root temperature reduced total upward transport of the mineral nutrients and phytohormones, most probably because of reduced water flow through the plant.
Taylor and Francis Inc., 1998年, Journal of Plant Nutrition, 21 (7), 1463 - 1481, 英語[査読有り]
研究論文（学術雑誌）
The first total synthesis of sorgolactone is reported, which confirms the proposed structure of the naturally occurring germination stimulant.
1997年03月31日, Tetrahedron Letters, 38 (13), 2321 - 2324, 英語[査読有り]
研究論文（学術雑誌）
The effect of cytochrome P-450 inhibitors on biosynthesis of benzylisoquinoline alkaloids, in cultured roots of Stephania cepharantha Hayata and Menispermum dauricum DC., was studied. In S. cepharantha only two alkaloids, aromoline and berbamine, were produced. Most inhibitors reduced root growth and alkaloid biosynthesis. Alkaloid contents were positively correlated with root growth (r=0.82 and 0.78 for aromoline and berbamine, respectively). In M. dauricum ancymidol and metyrapone promoted root growth, ketoconazole was inhibitory, while other inhibitors had inconsistent effects. Production of the alkaloids dauricine and acutumine was curtailed by all inhibitors. Alkaloid contents were not related to root growth. None of the inhibitors induced accumulation of the immediate monomeric precursors of benzylisoquinoline. However, ketoconazole-treated M. dauricum roots accumulated tyramine, an early precursor of benzylisoquinoline, and two unidentified tyrosine-derived alkaloids with molecular masses of 353 and 426.
Elsevier GmbH, 1997年, Journal of Plant Physiology, 150 (4), 376 - 380, 英語[査読有り]
研究論文（学術雑誌）
Cultured roots of Menispermum dauricum, a rich source of the bisbenzylisoquinoline alkaloid dauricine (1), were fed with L-[U-^<14>C]tyrosine, L-[3-^<13>C] tyrosine, and [2-^<13>C] tyramine independently, and the incorporation of possible early precursors into 1 was studied. The results demonstrdted that 1 was composed of four molecules of tyrosine, and that tyramine was specifically incorporated into the isoquinoline portions of 1. The unusual chlorine-containinig alkaloid acutumine (3), into which ^<14>C-labeled tyrosine was also incorporated, was identified as onie of the main conistituents in the alkaloid fraction from the roots.
社団法人日本農芸化学会, 1996年03月, Bioscience, biotechnology, and biochemistry, 60 (3), 503 - 505, 英語[査読有り]
研究論文（学術雑誌）
Drought tolerance is an important crop characteristic for maintenance of productivity under water deficit conditions. INterspecific differences among four gramineous crops (barnyard millet, maize, pearl miller and sorghum) in growth response to soil drying were studied. Seeds were sown in a sandy soil. Irrigation was stopped in some plots 16 days after sowing and was continued in others. Stopping irrigation increased resistance to water flow to between 2- and 21-fold. However, it decreased the soil water potential by -0.004 to - 12.7 MPa, the relative growth rate (RGR) by 15 to 27% the net assimilation rate (NAR) by 17 to 34% and the photosynthetic rate by 16 to 45%, respectively. Pearl miller and sorghum, which were identified as drought tolerant, displayed the lowest reductions in RGR. RGR was predominantly limited by NAR in all crops. The photosynthetic rate was preponderantly limited by stomatal conductance. Stomatal conductance correlated with leaf xylem water potential significantly. Pearl millet and sorghum showed the highest leaf water status. Root systems of all crops reached 140 cm soil depth. Under water stress, total length was significantly reduced in maize, was not affected in barnyard millet, and was significantly increased in sorghum and pearl millet. Drought tolerance in sorghum and pearl millet was associated with sustained water uptake ability by increasing total root length and maintenance of high leaf water status under soil drying conditions at the vegetative growth stage.
Crop Science Society of Japan, 1996年, Japanese Journal of Crop Science, 65 (2), 352 - 360, 英語[査読有り]
研究論文（学術雑誌）
Tomato (Lycopersicon esculuntum Mill.) grown in open fields in dry land areas or in non-controlled greenhouses are subjected to substantial daily changes in root temperature. In the field, root-zone temperatures fluctuate both diurnally and during the growing season. The purpose of this study was to monitor root-zone temperature effects on tomato initial growth, transpiration, sap flow rate, leaf and air temperatures differences, nitrate accumulation, total nitrogen, and soluble carbohydrates in the shoot and roots as well as levels of endogenous cytokinins and gibberellins in xylem exudate. Tomato seedlings were grown in three growth cabinets with variable control of root temperatures. Three day/night root temperature regimes (12/12, 16/8 and 20/20°C) were employed. Low day root temperatures of 12 and 16°C reduced shoot dry weight by 47 and 26%, root dry weight by 36 and 14%, shoot nitrate by 79 and 50%, root nitrate by 49 and 16%, levels of cytokinins in root xylem exudate by 27 and 13% and gibberellins by 65 and 23%, in relation to the respective values of 20°C day root temperature. Soluble carbohydrates in the shoot and roots were increased significantly (18 and 111%) by 12°C root temperature. The main effects of low root temperatures on shoot growth stem from slow upward transport of plant hormones and nitrate rather than reduction in their rate of biosynthesis or entry to the root, respectively.
Marcel Dekker Inc., 1996年, Journal of Plant Nutrition, 19 (3-4), 619 - 634, 英語[査読有り]
研究論文（学術雑誌）
1995年12月, Planta Medica, 61 (6), 497 - 501, 英語Inhibitory effects of bisbenzylisoquinoline alkaloids on induction of proinflammatory cytokines, interleukin-1 and tumor necrosis factor-[査読有り]
研究論文（学術雑誌）
Tomato (Lycopersicon esculentum Mill.) is commercially grown in a wide range of climatic conditions. There is a need to understand nutrient uptake by tomato at various climatic conditions in order to devise the specific nutrient supply to this important crop. Tomato seedlings, at the first flower blooming stage, were transplanted into 6-L pots of washed sand. Four root temperature regimes (day/night, °C) 12/12, 16/08, 17/17, and 30/20, and four temperatures of the shoots 20/20, 25/25, 25/15, and 30/20 were controlled separately for the shoots and roots. All four growth cabinets had the same day length of 13 h and light intensity of 400 μmole/m2/s measured on top of the canopy, The relative humidity was 85%. The nutrient solutions contained 10 mM N in three ratios of nitrate:ammonium (NO3:NH4)—10:0, 8:2, and 6:4 each in three replications in each growth cabinet. The water consumption, fresh and dry weight, water soluble nutrient in the plant, exudate composition at the end of the experiment and total mineral composition in the shoots were determined. The transpiration ratio for the high root temperatures was 190 g H20/g DM. Based on rate of water uptake, plant growth on the 16/08°C root regime was significantly higher than that of 12/12°C root regime, despite the fact that the average temperature on time was the same in both treatments. Low root temperatures decreased the concentrations of cations in the plant but had no effect on the balance between cations and anions (C-A). Increasing NH4 proportion decreased C-A in the plant. At root temperature of 12°C, potassium (K) and NO3were present at high concentration in the exudate but soluble NO3in the shoot was the lowest. The result suggests that nitrate reduced in the shoot produced organic anions that are left in the shoot and their charge balance that on K, due to slow root growth at low temperatures. Higher DM was produced by the NO3only form at low variable root temperature than in the treatments that contained NH4. At low root temperature, the K selectivity against sodium (Na) was reduced. This suggest that, at low root temperatures, high K concentration in the nutrient solution might he needed. © 1994, Taylor & Francis Group, LLC. All rights reserved.
1994年10月01日, Journal of Plant Nutrition, 17 (11), 2001 - 2024, 英語[査読有り]
研究論文（学術雑誌）
Root Cultures of Menispermum dauricum were established from adventitious roots formed on the leaf segments. These roots produce dauricine as a major constituent and the optimum conditions for dauricine production in cultured roots were investigated. A dauricine content of more than 0.5% dry weight was achieved in Gamborg's B5 medium containing 3% sucrose and 7.5 μM NAA. Tyrosine and tyramine, possible precursors of dauricine, increased dauricine formation. There was a positive correlation between dauricine content and root growth. © 1994.
1994年, Phytochemistry, 36 (3), 679 - 683, 英語[査読有り]
研究論文（学術雑誌）
1993年10月, Planta Medica, 59 (5), 475 - 476, 英語Vasodilator effects of bisbenzylisoquinoline alkaloids from Stephania cepharantha[査読有り]
研究論文（学術雑誌）
Cultured roots of Stephania cepharantha, rich in the bisbenzylisoquinolines aromoline (1) and berbamine (2), were fed [3‐13 C]tyrosine. The labelled alkaloids were isolated. 13C NMR spectra showed that 13C was specifically incorporated into sites on 1 and 2. The ratios of 13C enrichment for the benzyl and isoquinoline portions of 1 and 2 were the same within the experimental limits, evidence that both alkaloids are derived from a common intermediate. Copyright © 1993 John Wiley & Sons, Ltd
1993年, Phytochemical Analysis, 4 (3), 100 - 102, 英語[査読有り]
研究論文（学術雑誌）
Synthesis of IL-1β and TNFα by human monocytes-macrophages was significantly inhibited by eleven bisbenzylisoquinolines and one half-molecule (benzylisoquino-line), with IC50 values in the μM range. The results indicate that these compounds may have value in the therapy of human diseases where these inflammatory cytokines have a central role in pathogenesis. © 1993, Rapid Communications of Oxford Ltd.
1993年, Mediators of Inflammation, 2 (3), 199 - 203, 英語[査読有り]
研究論文（学術雑誌）
Cultured roots of Stephania cepharantha, rich sources of the bisbenzylisoquinolines aromoline [1] and berbamine [2], were fed [2-13C] tyramine and the labelled alkaloids isolated. The 13C-nmr spectra showed that 13C was specifically incorporated into the isoquinoline portions of 1 and 2. The results suggest that either the hydroxylation of tyramine proceeds more rapidly than its oxidation or that tyrosine conversion to the benzyl moiety proceeds via its corresponding α-keto acid. © 1993, American Chemical Society. All rights reserved.
1993年, Journal of Natural Products, 56 (8), 1229 - 1233, 英語[査読有り]
研究論文（学術雑誌）
1992年12月, Planta Medica, 58 (6), 505 - 508, 英語Histamine release inhibition activity of bisbenzylisoquinoline alkaloids[査読有り]
研究論文（学術雑誌）
Cultured roots of Stephania cepharantha, which are rich sources of bisbenzylisoquinoline alkaloids, were fed 14C-labelled tyrosine, tyramine or dopamine. While tyrosine was well incorporated into the bisbenzylisoquinolines, tyramine and dopamine were poorly incorporated. Incorporated tyrosine was shown to be decarboxylated and stored as tyramine in the roots, then gradually converted to the bisbenzylisoquinolines. Tracer experiments using [3-13C]tyrosine demonstrated that tyrosine was specifically incorporated into the corresponding sites of aromoline, which verified that aromoline was composed of four molecules of tyrosine. The ratio of 13C-enrichments of C-4 and C-α in (R) and (S) halves of aromoline was the same within experimental limits. This indicated that the two coclaurine units must have one and the same biogenetic origin. © 1990.
1990年10月29日, FEBS Letters, 273 (1-2), 82 - 86, 英語[査読有り]
研究論文（学術雑誌）
1989年02月, Journal of Natural Products, 52 (1), 192 - 202, 英語Comparison of bisbenzylisoquinoline alkaloids in root cultures of Stephania cepharantha with those in the parent plant[査読有り]
研究論文（学術雑誌）
Bisbenzylisoquinoline alkaloid production was studied in roots of Stephania cepharantha (Menispermaceae). Various cultural conditions influenced alkaloid production. Low concentrations of gibberellin suppressed browning of the cultured roots, and stimulated their growth and the production of bisbenzylisoquinoline alkaloids. Reducing the major element concentration of B5 medium by half increased root growth and alkaloid content. Elimination of the ammonium sulfate from B5 medium also increased the root alkaloid content, but it decreased growth as well. The optimal sucrose concentration was 3%. Cytokinin did not promote alkaloid production. As a result of these investigations, a modified B5 medium (SB5 medium) was developed for the production of bisbenzylisoquinoline alkaloids by cultured roots of S. cepharantha. The cultured roots grew well in this medium, with about a 20-fold dry weight increase in 30 days. The aromoline content in cultured roots was more than 1% and berbamine content more than 0.5% of the dry wt. The alkaloid levels of these cultured roots are much higher than those of the plants. © 1988, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.
1988年, Agricultural and Biological Chemistry, 52 (6), 1495 - 1498, 英語[査読有り]
研究論文（学術雑誌）
Roots obtained from the callus and tuber of Stephania cepharantha were cultured. These roots produced at least five bisbenzylisoquinoline alkaloids homoaromoline, aromoline, isotetrandrine, berbamine and cycleanine. In each cell line, the main constituents were always aromoline and berbamine. There was a high, positive correlation between the aromoline and berbamine contents. © 1988.
1988年, Phytochemistry, 27 (5), 1379 - 1381, 英語[査読有り]
研究論文（学術雑誌）
Growth and Aspartate Kinase Activity in Wheat Cell Suspension Cultue : Effects of Lysine Analogs and Aspartate-Derived Amino Acids :The effects of lysine analogs and aspartate-derived amino acids on the growth of wheat cell suspension culture were studied. S-(2-Aminoethyl)-L-cysteine (AEC), δ-hydroxylysine (DHL) and trans-lysene caused complete growth inhibition at 1.0 mM. The growth inhibition of lysine analogs were, in the order of decreasing effectiveness; AEC≥DHL, trans-lysene>oxalysine, homolysine and lysyne. cis-Lysene and methyl-lysine were not inhibitory even at concentrations of 10 mM. Lysine effectively relieved growth inhibition induced by the lysine analogs. Lysine plus threonine showed concerted inhibition, which was relieved by the addition of methionine. Activity of aspartate kinase extracted from wheat cell suspension culture was strongly inhibited by L-lysine; 0.75 to 1 mM of lysine was required for half-maximal inhibition. Threonine and methionine, individually or in combination with lysine, showed no inhibitory effect on the enzyme activity. S-Adenosylmethionine, when added with lysine in equimolar concentrations, enhanced the feedback inhibition by lysine, lowering the concentration of lysine for half-maximal inhibition to 0.13 mM. The aspartate kinase isolated from the cells cultured in the presence of 5 mM lysine did not differ in regulatory properties from the enzyme from the cells cultured without lysine. AEC at 5 mM inhibited the enzyme activity by 50%. Other lysine analogs were not inhibitory to the enzyme activity even at 10 mM. Growth inhibition of wheat suspension culture by aspartate-derived amino acids and lysine analogs were discussed in relation to their inhibitory effects on aspartate kinase activity.
Japanese Society of Plant Physiologists, 1986年06月, Plant and cell physiology, 27 (4), 607 - 617, 英語[査読有り]
研究論文（学術雑誌）
Japan Society for Bioscience, Biotechnology, and Agrochemistry, 1983年11月, Agricultural and Biological Chemistry, 47 (11), 2685 - 2687, 英語[査読有り]
研究論文（学術雑誌）
MISC
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研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2016年09月01日, 日本植物学会大会研究発表記録, 80th, 178, 日本語ナス科作物のステロイドグリコアルカロイド生合成遺伝子を標的としたゲノム編集植物の解析
- 2016年08月20日, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 34th, 72, 日本語ステロイドグリコアルカロイド生合成遺伝子CYP88B1をターゲットとしたゲノム編集ジャガイモの解析
- 2016年08月, 学術の動向, 21 (8), 18 - 23, 日本語根寄生雑草の潜在的危険性と生活環に着目した防除の試み
[招待有り]
記事・総説・解説・論説等（学術雑誌）
- 2016年07月, バイオサイエンスとバイオインダストリー, 74 (4), 314 - 315, 日本語根寄生雑草防除に向けた自殺発芽誘導剤の開発と実証試験
[招待有り]
記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
- 2016年03月29日, 日本農芸化学会2016年度大会，札幌, 日本語ナス科植物の有毒ステロイドグリコアルカロイド生合成に関わるアミノトランスフェラーゼの機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2016年03月19日, 第57回日本植物生理学会年会，岩手, 日本語ナス科植物ステロイドグリコアルカロイドのF環形成に関わる26位アミノ基転移酵素の機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2016年03月05日, 日本農芸化学会大会講演要旨集(Web), 2016, 2H043 (WEB ONLY), 日本語ステロイドグリコアルカロイド生合成遺伝子SGA3をターゲットとしたゲノム編集トマトの解析
- 2016年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 34thジャガイモのα-ソラニン生合成遺伝子と共発現する機能未知遺伝子の解析
- 2016年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 34thサポニン生合成遺伝子過剰発現によるトマトのトマチン生産能の増強
- 2016年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 34thヤマノイモ属トゲドコロのステロイドサポニン生合成に関わる糖転移酵素の探索
- 2016年, 日本植物学会大会研究発表記録, 80th沖縄特産トゲドコロのステロイドサポニン生合成の解析
- 2016年, 日本農芸化学会大会講演要旨集(Web), 2016トマトにおけるα-トマチン生合成に関わるステロイド3位酸化異性化酵素の解析
- 2016年, 日本植物学会大会研究発表記録, 80thトマトにおけるα-トマチン生合成に関わるA環酸化還元酵素の機能解析
- 2016年, 日本農芸化学会大会講演要旨集(Web), 2016ヤマノイモ属のステロイドサポニン生合成に関わるCYP90B遺伝子の機能解析
- 2016年, 日本農芸化学会関西支部講演会講演要旨集, 497thシロイヌナズナの酸化ストレス情報伝達系に関わるシグナル受容体の探索
- 2016年, 関東雑草研究会報, 27, 5 - 19, 日本語根寄生雑草の生存戦略の化学的解明と防除への応用
[査読有り][招待有り]
記事・総説・解説・論説等（学術雑誌）
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 117 - 117, 日本語P099 アブシジン酸に対するストライガの発芽と気孔の応答(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 116 - 116, 日本語P098 根寄生雑草ストライガの宿主からの同化産物の収奪にはアブシジン酸に対する気孔応答の異常が関与している(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 107 - 107, 日本語P089 トマトにおけるα-トマチン生合成に関わるステロイド3位酸化異性化酵素の解明(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 55 - 55, 日本語P037 ササゲにおけるorobanchol生合成遺伝子の解明に向けたRNA-seq解析(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 54 - 54, 日本語P036 様々な植物におけるストリゴラクトン生合成の多様性の解析(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 53 - 53, 日本語P035 ソルガムにおけるSorgomol合成酵素遺伝子の同定及び機能解析(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 52 - 52, 日本語P034 植物による5-deoxystrigol立体異性体の水酸化反応とストリゴラクトン前駆体の探索(ポスター発表,植物化学調節学会第50回大会)
- 植物化学調節学会, 2015年10月01日, 植物化学調節学会研究発表記録集, 50, 97 - 97, 日本語P079 傷害時に放出される2-ヘキセナールを生合成するヘキセナールイソメラーゼの同定と機能解析(ポスター発表,植物化学調節学会第50回大会)
- 2015年08月11日, 第33回日本植物細胞分子生物学会大会，東京, 日本語ナス科植物ステロイドグリコアルカロイド生合成に関わるアミノ基転移酵素の機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2015年03月27日, 日本農芸化学会2014年度大会，岡山, 日本語ナス科植物ステロイドグリコアルカロイドの構造多様性に関わる生合成酵素の機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2015年, 日本植物生理学会年会要旨集, 56thナス科植物ステロイドサポニンのF環の構造多様性に関わるコレステロール26位水酸化酵素の機能解析
- 2015年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 33rdα-トマチン生合成に関わる3位酸化異性化酵素の解析
- 2015年, 植物の生長調節, 50 (Supplement)トマトにおけるα-トマチン生合成に関わるステロイド3位酸化異性化酵素の解明
- 2015年, 熱帯農業研究, 8 (1), 11 - 14, 日本語根寄生雑草ストライガの防除方法に関する研究成果とスーダン国ガダーレフ州農家への普及活動
[査読有り]
- 植物化学調節学会, 2014年10月01日, 植物化学調節学会研究発表記録集, 49, 85 - 85, 日本語67. ヒマワリの生産する非セスキテルペンラクトン型根寄生雑草発芽刺激物質heliolactoneの同定(口頭発表,植物化学調節学会第49回大会)
- 植物化学調節学会, 2014年10月01日, 植物化学調節学会研究発表記録集, 49, 87 - 87, 日本語69. ソルガムにおけるGR24水酸化反応の立体特異性と位置選択性(口頭発表,植物化学調節学会第49回大会)
- 植物化学調節学会, 2014年10月01日, 植物化学調節学会研究発表記録集, 49, 96 - 96, 日本語78. 自殺発芽誘導によるStriga hermonthica抑制の実証(口頭発表,植物化学調節学会第49回大会)
- 植物化学調節学会, 2014年10月01日, 植物化学調節学会研究発表記録集, 49, 68 - 68, 日本語50. 防御シグナル因子ピペコリン酸の生合成に関わるALD1の機能解析(口頭発表,植物化学調節学会第49回大会)
- 2014年09月20日, 2014年度日本農芸化学会関西支部大会, 日本語ナス科植物ステロイドサポニンの構造多様性に関わるステロイド26位水酸化酵素の機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2014年03月29日, 日本農芸化学会2014年度大会，東京, 日本語ジャガイモの有毒ソラニン生合成に関わる新規ジオキシゲナーゼの機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2014年03月18日, 第55回日本植物生理学会，富山, 日本語ナス科植物ステロイドグリコアルカロイド生合成に関わる16位水酸化を触媒するジオキシゲナーゼの機能解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2014年03月, 日本農芸化学会2014年度大会，東京, 日本語ヤマノイモ属トゲドコロに蓄積するステロイドサポニンの単離精製および定量解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2014年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 32ndトマト果実におけるα-トマチン代謝酵素の探索
- 2014年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 32ndナス科植物ステロイドグリコアルカロイド生合成に関わるジオキシゲナーゼの機能解析
- 2014年, 日本農薬学会大会講演要旨集, 39th根寄生雑草ヤセウツボの発芽時に発現するβ-マンノシダーゼの役割
- 2014年, 日本農芸化学会大会講演要旨集(Web), 2014ヤセウツボ発芽種子で発現する糖加水分解酵素の機能解析
- 植物化学調節学会, 2013年10月04日, 植物化学調節学会研究発表記録集, 48, 71 - 71, 日本語56. S. gesnerioides D14-like遺伝子の探索とストライゴラクトン加水分解活性の評価(口頭発表,植物化学調節学会第48回大会)
- 植物化学調節学会, 2013年10月04日, 植物化学調節学会研究発表記録集, 48, 65 - 65, 日本語50. 植物における5-デオキシストリゴールからモノヒドロキシストリゴラクトンへの変換反応(口頭発表,植物化学調節学会第48回大会)
- 植物化学調節学会, 2013年10月04日, 植物化学調節学会研究発表記録集, 48, 60 - 60, 日本語45. ヒマワリが分泌する根寄生雑草発芽刺激物質の解析(口頭発表,植物化学調節学会第48回大会)
- 植物化学調節学会, 2013年10月04日, 植物化学調節学会研究発表記録集, 48, 84 - 84, 日本語69. ヤマノイモ属トゲドコロのステロイドサポニン生合成に関わるステロール22位水酸化酵素の同定(口頭発表,植物化学調節学会第48回大会)
- 植物化学調節学会, 2013年10月04日, 植物化学調節学会研究発表記録集, 48, 64 - 64, 日本語49. β-カロテン生産菌を用いたストライゴラクトン生合成中間体カルラクトン酵素合成の試み(口頭発表,植物化学調節学会第48回大会)
- 植物化学調節学会, 2013年10月04日, 植物化学調節学会研究発表記録集, 48, 103 - 103, 日本語
- 2013年10月, 植物化学調節学会第48回大会，新潟, 日本語ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成 16 位水酸化酵素の同定
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2013年09月, 第31回日本植物細胞分子生物学会大会，札幌, 日本語Identification and Quantification of Steroidal Saponins in Dioscorea esculenta
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 2013年03月28日, 日本作物學會紀事, 82, 64 - 65, 日本語ライゾトロン法でストライガ抵抗性を示した陸稲品種の野外試験での評価
- 日本農薬学会, 2013年03月14日, 講演要旨集, (38), 106 - 106, 日本語C214 ノジリマイシンによる根寄生雑草ヤセウツボ種子の発芽と糖代謝への影響(作用機構 抵抗性 代謝,分解,動態,一般講演要旨)
- 2013年, 植物の生長調節, 48 (Supplement)ヤマノイモ属トゲドコロのステロイドサポニン生合成に関わるステロール22位水酸化酵素の同定
- 2013年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 31stヤマノイモ属トゲドコロ由来のフロスタノール配糖体を加水分解するβ-グルコシダーゼの同定
- 2013年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 31stトマトにおけるステロイドアルカロイド生合成に関わる配糖化酵素の探索
- 2013年, 日本農芸化学会大会講演要旨集(Web), 2013ヤマノイモ属における有用ステロイドサポニン生合成に関わるβ-グルコシダーゼの同定
- 2013年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 31stヤマノイモ属トゲドコロ由来NADPH-P450還元酵素遺伝子の単離同定
- 2013年, 植物の生長調節, 48 (Supplement)ナス科植物ステロイドグリコアルカロイド生合成に関わる新規2-オキソグルタル酸依存性ジオキシゲナーゼ:16位水酸化酵素の同定と機能解析
- 2013年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 31stトマト果実におけるα-トマチン代謝酵素遺伝子の探索
- 2013年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 31stヤマノイモ属トゲドコロにおけるステロイドサポニン生合成P450遺伝子の探索
- 2013年, 日本農芸化学会大会講演要旨集(Web), 2013ヤセウツボ発芽種子で発現する糖加水分解酵素の機能解析
- 2013年01月, 化学と生物, 51, 36 - 42, 日本語「ストリゴラクトンの生物活性を担う立体化学の重要性」
- 一般社団法人 植物化学調節学会, 2013年, 植物の生長調節, 48 (0), 86 - 86, 日本語
- 2012年10月27日, 植物化学調節学会第47回大会，鶴岡, 日本語ナス科植物におけるステロイドグリコアルカロイド生合成に関わるP450の酵素解析
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 植物化学調節学会, 2012年10月05日, 植物化学調節学会研究発表記録集, 47, 68 - 68, 日本語51. ヒマワリが生産するストライゴラクトンの探索(口頭発表,植物化学調節学会第47回大会)
- 植物化学調節学会, 2012年10月05日, 植物化学調節学会研究発表記録集, 47, 64 - 64, 日本語47. Strigolactone の生理活性における最小構造(口頭発表,植物化学調節学会第47回大会)
- 植物化学調節学会, 2012年10月05日, 植物化学調節学会研究発表記録集, 47, 79 - 79, 日本語62. ソルガムによる5-deoxystrigol からsorgomol への変換(口頭発表,植物化学調節学会第47回大会)
- 植物化学調節学会, 2012年10月05日, 植物化学調節学会研究発表記録集, 47, 66 - 66, 日本語49. Striga gesnerioides 種子に対する発芽阻害物質の探索(口頭発表,植物化学調節学会第47回大会)
- 植物化学調節学会, 2012年10月05日, 植物化学調節学会研究発表記録集, 47, 80 - 80, 日本語63. 様々な植物における5-deoxystrigol の酸化的代謝(口頭発表,植物化学調節学会第47回大会)
- 2012年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 30thヤマノイモ属における有用ステロイドサポニン生合成に関わるβ-グルコシダーゼの解析
- 2012年, 日本農芸化学会大会講演要旨集(Web), 2012ヤマノイモ属トゲドコロにおけるプロトジオシンを加水分解するβ-グルコシダーゼの解析
- 54. 水酸化GR24の合成と構造解析(口頭発表,植物化学調節学会第46回大会)
To identify hydroxylated products of GR24, the synthesis of 5-, 6-, 7- and 8-hydroxy GR24 was undertaken. First, 5- and 6-hydroxy-1-indanone 1, 2 were protected with a MOM group, gave MOM protected indanone 3, 4, respectively. Indanone 3 and 4 reacted with dimethyl carbonate in the presence of sodium hydride, then ethyl bromoacetate gave compounds 5 and 6. Acid catalyzed hydrolysis and subsequent decarboxylation gave carboxylic acids 7 and 8. Then, sodium borohydride reduction, followed by acid treatment gave tricyclic lactones 9 and 10, respectively. Formylation of ABC-ring and coupling with D-ring are ongoing.
植物化学調節学会, 2011年10月03日, 植物化学調節学会研究発表記録集, (46), 70 - 70, 日本語 - 56. ストライゴラクトンの枝分かれ抑制活性における構造要求性(口頭発表,植物化学調節学会第46回大会)
Strigolactones (SLs) act as a phytohormone which inhibit shoot branching. Naturally occurring SLs such as 5-deoxystrigol consist of tricyclic ABC-ring and D-ring connected with enol ether bridge. Synthetic SL analogues such as GR24 were known to exhibit similar activity as natural SLs. However, it is still unclear whether SLs themselves are the active form or precursors of active principles. To get insight into the structure essential for inhibiting shoot branching, information about SL structure-activity relationship is necessary. In this study, we employed over 20 ABC-ring analogues of SL and tested their activity toward rice d10 mutant grown in hydroponic system. Based on the branching bioassay, D-ring and enol ether bridge structure were found to be critical but the AB-ring moiety is less important on SL activity. Furthermore, compounds having phenyl ether as a substitute for enol ether exhibited high activity, but the replacement of enol ether by benzyl ether resulted in the loss of activity. These results imply that D-ring and π-conjugated system are required for shoot branching inhibition.
植物化学調節学会, 2011年10月03日, 植物化学調節学会研究発表記録集, (46), 72 - 72, 日本語 - 55. ソルガムにおける5-deoxystrigolの酸化的代謝(口頭発表,植物化学調節学会第46回大会)
Strigolactones have been isolated from root exudates of various plants. A series of strigolactones may be derived from 5-deoxystrigol by oxidation, acetylation, methylation and demethylation. We found that some plants can convert GR24 to its hydroxylated products. In this study, 5-deoxystrigol was applied to hydroponically grown sorghum (Sorghum bicolor), and the conversion of the probable common precursor of other strigolactones to hydroxylated strigolactones was analyzed by LC-MS/MS. A sorghum variety referred to as NM24 was selected as the highest producer of sorgomol (9-hydroxylated 5-deoxystrigol). NM24 converted [6'-D]-5-deoxystrigol and [6'-D]-2'-epi-5-deoxystrigol to [6'-D]-sorgomol and its 2'-epimer, respectively. Accordingly, the ability to hydroxylate 5-deoxystrigol at C-9 in sorghum was confirmed.
植物化学調節学会, 2011年10月03日, 植物化学調節学会研究発表記録集, (46), 71 - 71, 日本語 - 58. 根寄生雑草Striga gesnerioides種子発芽を誘導するササゲ由来ストライゴラクトンの単離同定(口頭発表,植物化学調節学会第46回大会)
Alectrol was originally isolated from root exudates of cowpea (Vigna unguiculata) as a germination stimulant for Striga gesnerioides and Alectra vogelii. The strigolactone was also isolated from red clover (Trifolium pretense) as a stimulant for Orobanche minor and identified most probably as (+)-orobanchyl acetate. However, authentic (+)-orobanchyl acetate induced negligible S. gesnerioides seed germination. In this study, the germination stimulants for seeds of S. gesnerioides produced by cowpea were reinvestigated. Root exudates of cowpea were collected and fractionated. Two germination stimulants were detected. Based on chromatographic behavior on HPLC and physicochemical properties including CD and NMR spectra, structures of the isolated stimulants were determined as ent-2'-epi-orobanchol and ent-2'-epi-orobanchyl acetate. The same strigolactones were identified in root exudates of red clover.
植物化学調節学会, 2011年10月03日, 植物化学調節学会研究発表記録集, (46), 74 - 74, 日本語 - 98. ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成に関与する遺伝子の探索(口頭発表,植物化学調節学会第46回大会)
Steroidal saponins have various biological activities. Steroidal saponins are often found in monocotyledons such as plants of the families Dioscoreaceae, Agavaceae, and Liliaceae. In particular, the rhizomes of Dioscorea, known as yam, contain furostane and spirostane glycosides such as protodioscin and dioscin, respectively. These steroid saponins are derived from choresterol by sequential modification with oxygenation and transglycosylation reaction as shown in Figure 1. Namely, several P450s are likely involved in oxygenations at the C-16, C-22, and C-26 positions, and UGTs will function in transglycosylation at C-3 and C-26. But little is known about enzyme and genes for dioscin biosynthesis. To investigate steroidal saponin biosynthesis in Dioscorea spp., we performed comparative transcriptome analysis of the rhizomes of Dioscorea spp. We will utilize these datasets to identify key genes for cholesterol and dioscin biosynthesis, and will apply the results to plant metabolic engineering of steroidal saponins.
植物化学調節学会, 2011年10月03日, 植物化学調節学会研究発表記録集, (46), 114 - 114, 日本語 - 2011年03月30日, 日本作物學會紀事, 80, 52 - 53, 日本語ライゾトロン法およびスーダン現地でのポット試験による陸稲品種のストライガ感受性の評価
- 2011年03月30日, 日本作物學會紀事, 80, 380 - 381, 日本語異なる土壌水分条件下での根寄生雑草ストライガとソルガムの気孔反応と光合成活性
- 2011年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 29thヤマノイモ属トゲドコロにおけるステロイドサポニン生合成酵素の探索
- 2011年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 29thトマトにおけるα-トマチン生合成酵素の探索
- 2011年, 植物の生長調節, 46 (Supplement)ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成に関与する遺伝子の探索
- 2011年, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 29thトマト果実におけるα-トマチンの代謝酵素の探索
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 119 - 119, 日本語2Ba08 寄生植物ヤセウツボの発芽種子における希少三糖ゲンチアノースの代謝経路の解明(バイオマス・資源・エネルギー工学/植物細胞工学・組織培養・育種工学/有機化学・高分子化学,一般講演)
Parasitic weeds, Striga spp. and Orobanche spp. cause serious damage to agriculture worldwide. A novel and effective strategy for parasitic weed control is desired for economical and humanitarian reasons. We focused on the germination process of parasitic weeds to find biological events specific to these species and conducted metabolic profiling of Orobanche minor seeds. Consequently, we revealed that gentianose, a rare trisaccharide consisted of two glucose and a fructose, was decreased concurrently with increases of glucose and fructose immediately after GR24 treatment. A potent inhibitor of glycosyl hydrolases, nojirimycin bisulfite, decreased not only the germination rate of O. minor but also that of Striga gesnerioides. From these results, we hypothesize that gentianose metabolism is crucial for germination of these parasitic weeds and the key enzyme in the gentianose metabolic pathway could be a novel target for selective control of parasitic weeds. To characterize the enzymes involved in gentianose metabolism, we extracted crude enzyme from O. minor seeds and measured glycosyl hydrolyzing activity. As a result, it is revealed gentianose is hydrolyzed to monosaccharides via disaccharide gentiobiose. The gentiobiose hydrolyzing activity in the cell wall/membrane binding protein fraction was increased during the germination process and reached a maximum 4 days after GR24 treatment. Additionally, gentiobiose hydrolyzing enzyme was inhibited selectively by nojirimycin.
一般社団法人 植物化学調節学会, 2011年, 植物化学調節学会 研究発表記録集, 46, 76 - 76, 日本語- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 166 - 166, 日本語2Ha02 放線菌Streptomyces ficellusによる寄生植物種子発芽阻害剤ノジリマイシンの生産(発酵生理学・発酵工学,一般講演)
- 80.キュウリ葉からのα,β-不飽和カルボニル化合物還元酵素の精製と遺伝子解析(口頭発表)
Polyunsaturated fatty acids in biomembranes are oxidized easily, and result in various low-molecular weight compounds. These compounds include α,β-unsaturated carbonyls like acrolein and methylvinylketone, which are highly reactive. The toxicity of these reactive compounds are based on their ability to form the Michael adducts with thiols and amino groups in proteins and nucleic acids. Therefore, it is important to scavenge α,β-unsaturated carbonyls for maintaining homeostasis in plants. In this study, we purifed the acrolein-reducing enzyme from cucumber leaves and determined its partial amino acid sequences. Based on RT-PCR with degenerate primers and RACE strategies, two cDNA sequences contained fully functional region of the enzyme were obtained. One has chloroplast-targeted transit sequence at N-terminal region, and the other has no. These results indicate that cucumber leaves have α,β-unsaturated carbonyl-detoxifying enzymes localizing in chloroplast and cytosol.
植物化学調節学会, 2010年10月01日, 植物化学調節学会研究発表記録集, (45), 97 - 97, 日本語 - 83.植物におけるクマリン類生合成の鍵酵素である桂皮酸類オルト位水酸化酵素の解析(口頭発表)
Coumarins are ubiquitously found as the plant secondary metabolites in plants and are biosynthesized via ortho-hydroxylation of cinnamates by cinnamates 2'-hydroxylase (C2'H), which is a key enzyme in coumarins biosynthesis. We investigated C2'Hs from Arabidopsis and sweet potato (Ipomoea batatas), which accumulate the glucosides of scopoletin and umbelliferone, respectively. Previous studies showed that C2'H is a 2-oxoglutarate-dependent dioxygenase and catalyzes ortho-hydroxylation of the CoA-ester of cinnamates to yield coumarins. The results suggest that the chemical composition of coumarins in plant species is determined by the substrate specificity of C2'H in each of the plants. To understand coumarins biosynthesis in various plants, we have isolated a C2'H (LjC2'H) cDNA from Lotus japonicus, in which coumarins have not yet been found so far. We found that LjC2'H catalyzes ortho-hydroxylation of feruloyl-CoA and cafeoyl-CoA to form scopoletin and esculetin, respectively. We have also isolated a C2'H cDNA from grapefruit (Citrus x paradisi). Grapefruit accumulates furanocoumarins, which is derived from umbelliferone, and characterization of grapefruit C2'H is in progress. In addition, we have established bacterial biosynthesis of coumarins. Plant C2'Hs were co-expressed with 4-coumaroyl CoA:ligase in E. coli, and the feeding of cinnamates resulted in the accumulation of coumarins in the culture medium.
植物化学調節学会, 2010年10月01日, 植物化学調節学会研究発表記録集, (45), 100 - 100, 日本語 - 34.メスキート葉に由来する根寄生植物の種子発芽抑制物質の探索(口頭発表)
Mesquite (Prosopis juliflora) is the leguminous shrubs growing in the tropical or subtropical regions. In this study, we are searching for biologically active substances produced by mesquite leaves toward root parasitic plants. Dried mesquite leaves (250g) were extracted with methanol. After partitioning, the extract (48.6g) yielded crude alkaloids (3.9g), which exhibited the potent inhibitory activity for seed germination and radicle elongation of root parasitic plants Striga hermonthica and Orobanche minor. Bioassay-guided separation of the crude alkaloids by silica gel column chromatography, TLC and HPLC gave two active principles. Structural analysis of the compounds is in progress.
植物化学調節学会, 2010年10月01日, 植物化学調節学会研究発表記録集, (45), 51 - 51, 日本語 - 32.根寄生植物オロバンキの成長過程における脱分化・分化の制御機構の解明(口頭発表)
Orobanche minor seedlings were attached onto red clover (Trifolium pretense) roots grown in rhizotrons. Approximately 30% of the O. minor seedlings formed tubercles on red clover roots 2 weeks after attachment. Root primordia were appeared on the surface of the tubercles, and crown roots were developed 19 days after attachement. A shoot bud was differentiated from the crown roots 5 weeks after attachment. O. minor parasitism induced little morphological changes in red clover roots during the development process from tubercle formation (de-differentiation) to shoot bud formation (re-differentiation). These observations suggest that O. minor produces growth regulators in its own metabolism to control its differentiation. In addition, the crown roots and the shoot bud were differentiated without the seed embryo of O. minor, suggesting that the developmental process seems to be the process of adventitious root and shoot formation. To investigate the involvement of phytohormone homeostasis in the differentiation process, the endogenous levels of various plant hormones during the developmental process from seed germination to flowering were measured. Furthermore, gene expression involved in the developmental process of O. minor has been extensively studied with the next-generation sequencing platform.
植物化学調節学会, 2010年10月01日, 植物化学調節学会研究発表記録集, (45), 49 - 49, 日本語 - 86.Gentiana urnula Harry Sm.の花由来の新規抗酸化成分(口頭発表)
Objectives: Gentiana urnula Harry Sm. is a small perennial plant distributed in the high mountain regions (3700-5700m altitude) in western China and Himalaya, and its flower has been used traditionally as "Gang-gah Chung" in Tibetan medicine systems. Our extensive screenings of alpine plants in the regions have shown the flower of G. urnula contains strong antioxidants. Regarding the chemical ingredients, however, little information is currently available except for the presence of some iridoid compounds. This study, therefore, focused on the exploration of main antioxidants contained in the flower. Methods: Methanol extract from the dried flowers of G. urnula was used as a starting material for purification. The crude extract dispersed in water was extracted with ethyl acetate and subsequently with n-butanol. The n-butanol fraction was further purified by mid-pressure LC (Yamazen) and HPLC (Shiseido) using C18 reverse phase columns. DPPH radical scavenging assay was used for the evaluation of antioxidant activity. Three antioxidant compounds were purified and subjected for structural analyses (NMR and HR-MS). Results: Two compounds were identified as known iridoid glycosides (Gentiournoside A and Gentiournoside E). The other one, which exhibited the highest antioxidant activity, was a novel iridoid with MW 782. The content of the novel iridoid in the dried flower was calculated to be approximately 0.7w/w% from its recovery rate.
植物化学調節学会, 2010年10月01日, 植物化学調節学会研究発表記録集, (45), 103 - 103, 日本語 - 日本農薬学会, 2010年04月21日, 講演要旨集, (35), 114 - 114, 日本語3A08 寄生雑草ヤセウツボの発芽に関与する糖分解酵素に関する研究(作用機構・抵抗性(殺虫剤),一般講演要旨)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 130 - 130, 日本語2P-2081 寄生雑草ヤセウツボ種子の発芽とゲンチアノース代謝活性の相関解析(7a 植物細胞工学,組織培養,育種工学,一般演題,植物バイオテクノロジー,伝統の技と先端科学技術の融合)
Orobanche minor is an obligate root parasitic weed belonging to Orobanchaceae. Some root parasitic weeds of Orobanchaceae cause serious damage to many agronomically important crops worldwide. A novel and effective strategy for the parasitic weed control is desired for economical and humanitarian reasons. Since the life cycle of parasitic weeds is significantly different from that of host plants, understanding of the parasite-specific biological events is important for design of selective control strategies. We focused on the germination process of parasitic weeds to find biological events specific to these species and conducted metabolic profiling of O. minor seeds. Consequently, we revealed that gentianose was decreased immediately after GR24 treatment. Gentianose is a trisaccharide consisted of two glucoses and a fructose. An inhibitor of glycosyl hydrolases, nojirimycin bisulfite (NJ), decreased the germination rate. From these results, we hypothesize that gentianose metabolism is essential for germination of O. minor seed and the key enzyme in the gentianose metabolic pathway could be a novel target for selective control of parasitic weeds. In this study, we extracted crude enzymes from O. minor seeds and measured glycosyl hydrolyzing activity. We revealed that the enzyme involved in germination did not exist in the soluble fraction, but associated with the insoluble components, most likely the cell wall.
一般社団法人 植物化学調節学会, 2010年, 植物化学調節学会 研究発表記録集, 45, 50 - 50, 日本語- 38.メスキートが生産する有用二次代謝産物の検索(口頭発表)
Mesquite (Prosopis juliflora) is the leguminous shrubs growing in the tropical or subtropical regions. In this study, we conduct screening of biologically active substances produced by mesquite. Dried mesquite leaves (250g) were extracted with methanol. The extract (48.6g) was partitioned into the acidic and neutral fraction (22.2g) and the basic fraction (3.9g). The acidic and neutral fraction exhibited the inhibitory activity for root and shoot growth of lettuce seedlings. Furthermore, the basic fraction and the acidic and neutral fraction inhibited seed germination of Striga hermonthica, S. gesnerioides and Orobanche minor. The purification of these bioactive substances is in progress.
植物化学調節学会, 2009年10月06日, 植物化学調節学会研究発表記録集, (44), 52 - 52, 日本語 - 41.柑橘およびマメ科植物におけるクマリン化合物生合成酵素の探索(口頭発表)
Coumarins are ubiquitously found as the plant secondary metabolites in plants. They are thought to play important roles in plant defense due to their antimicrobial and antioxidative activities. Coumarins are biosynthesized via ortho-hydroxylations of cinnamates (C2'H). This is a key step in the lactone ring formation in coumarin biosynthesis, and C2'H is classified into the 2-oxoglutarate-dependent dioxygenase (2OGD) family. Arabidopsis roots accumulate scopoletin β-glucoside, and Arabidopsis C2'H shows the high substrate specificity to feruloyl-CoA but does not accept ferulic acid as a substrate. Sweet potato (Ipomoea batatas L.) tubers accumulate the β-glucosides of scopoletin and umbelliferone, and C2'H of sweet potato can accept feruloyl-CoA and p-coumaroyl-CoA as its substrates to form scopoletin and umberiferone, respectively. These results suggest that the substrate specificity of C2'H determines the accumulation patterns of coumarins in each plant species. In this study, we have found that there are C2'H homologs in the EST database of citrus and legume (Medicago truncatula and Lotus japonicus). Citrus fruits are known to accumulate furanocoumarins, but little is known about coumarin synthesis in M. truncatula and L. japonicus. In order to explore coumarin biosynthesis in citrus and the legume plants, we have performed the functional analysis of the C2'H homologs of these plants.
植物化学調節学会, 2009年10月06日, 植物化学調節学会研究発表記録集, (44), 55 - 55, 日本語 - 42.イソキノリンアルカロイド生合成に関わるシトクロムP450 cDNAの単離および機能解析(口頭発表)
In the biosynthesis of isoquinoline alkaloids, several unusual monooxygenation reactions such as the intermolecular C-O phenol-coupling and the intramolecular C-C phenol coupling reactions are involved, and cytochromes P450 such as CYP80A1 and CYP80G2 have been shown to catalyze these reactions. Root cultures of Stephania cephatantha produce several isoquinoline alkaloids, and, in this study, we have isolated five P450 cDNAs from the root cultures by RT-PCR with the degenerated primers specific to CYP80A1 and CYP80G2. The isolated P450s showed high amino-acid sequence identities with the CYP80 family members, suggesting their involvement in isoquinoline biosynthesis in S. cepharantha. Further analyses to determine the function of these P450 cDNAs with heterologous expression are now in progress.
植物化学調節学会, 2009年10月06日, 植物化学調節学会研究発表記録集, (44), 56 - 56, 日本語 - 47.ブラシノステロイド生合成に関与すると推定されるコケおよびシダ植物P450の機能解析(口頭発表)
Several cytochromes P450 (P450s) are involved in the biosynthesis of phtohormones in higher plants. Genome sequencing projects of Physcomitrella patents and Selaginella moellendorffii have revealed that some orthologous genes to phytohormone-related P450s from the flowering plants may be present in bryophyte and pteridophyte. We think that the comparative analysis of plant P450s conserved across the evolutionary stages can give us a clue to study the evolutionary aspects of phytohormone homeostasis. In this study, we performed functional analysis of putative brassinosteroid-biosynthetic P450s from P. patents and S. moellendorffii. We have found that CYP763A1 and CYP763B1/B2 from P. patents and CYP90E1/E2/E3 and CYP90F1 from S. moellendorffii may be the orthologs of brassinosteroid-biosynthetic P450s from the flowering plants. We have isolated the full-length cDNAs of the P450s except for CYP90F1 by RT-PCR. The recombinant P450 proteins were expressed in E. coli and with a baculovirusinsect cell system in order to analyze their catalytic activities in vitro. Now, we are looking for their proper substrates among the BR-biosynthesis intermediates and try to reveal the enzymatic reactions catalyzed by them.
植物化学調節学会, 2009年10月06日, 植物化学調節学会研究発表記録集, (44), 61 - 61, 日本語 - 60.根寄生植物の種子発芽刺激活性に対するストライゴラクトンB環の修飾の影響について(口頭発表)
The seeds of root parasitic plants Orobanche spp. and Striga spp. only germinate when they are exposed to stimulant molecules, collectively referred to as strigolactones. In this study, strigolactones and their synthetic analogs with modification at C-4 were evaluated for their potency in inducing seed germination of S. hermonthica, S. gesnerioides, and O. minor. We prepared three synthetic analogs, GR24, OH-GR24 having a hydroxy group at position C-4, and AcO-GR24 having an acetoxy group at C-4, and these compounds were used for the seed germination assay. In the S. hermonthica germination assay, the activity was GR24>OH-GR24 and AcO-GR24. In the O. minor germination assay, the activity was OH-GR24>GR24 and AcO-GR24. These results suggest that the modification at C-4 is an important factor to determine the activity and the specifity of strigolactones for the parasites. In contrast, S. gesnerioides seeds did not respond to any of the synthetic strigolactones while the seeds were responsive to cowpea root exudates that contain orobanchyl acetate as a stimulant. Although it is known that the CD-ring structure of strigolactones is essential for their bioactivity, the results obtained in this study suggested the importance of the A-ring structure for the bioactivity toward S. gesnerioides.
植物化学調節学会, 2009年10月06日, 植物化学調節学会研究発表記録集, (44), 74 - 74, 日本語 - 58.根粒共生がオロバンキの寄生に及ぼす影響(口頭発表)
Effects of rhizobial symbiosis on Orobanche aegyptiaca parasitism to Lotus japonicus were studied. Approximately 30% of the parasite attached onto the host root formed tubercles, and root primordia developed from the tubercles. Concurrent inoculation of Mesorhizobium loti doubled the number of tubercles, suggesting some positive effects of rhizobial symbiosis on the parasite invasion. Inoculation of the rhizobium three weeks before attachment of the parasite did not affect the number of tubercles. However, the tubercles turned brown, and root development of the parasite delayed significantly, suggesting the negative effects of the symbiosis on the parasite growth.
植物化学調節学会, 2009年10月06日, 植物化学調節学会研究発表記録集, (44), 72 - 72, 日本語 - 日本農薬学会, 2009年02月27日, 講演要旨集, (34), 140 - 140, 日本語9C23 選択的防除法に資する根寄生雑草発芽過程の代謝変動プロファイリング(天然物化学・作用機構・抵抗性(植調剤),一般講演要旨)
- 2009年, 植物の生長調節根寄生植物と宿主植物の相互作用に関する生物有機化学的研究
- 2009年, 植物の生長調節44（1）1-8根寄生植物と宿主植物の相互作用に関する生物有機化学的研究
- 12.根寄生植物に対するミヤコグサの応答の分子解析(口頭発表)
Lotus japonicus genes responsive to parasitism of compatible Orobanche aegyptiaca and incompatible Striga hermonthica were isolated and their expression was analyzed. cDNA libraries enriched by suppression subtractive hybridization (SSH) strategy for transcripts up-regulated in L. japonicus root segments adjacent to O. aegyptiaca tubercles and those adjacent to the attachment points of S. hermonthica seedlings have been established. Expression of genes isolated by SSH was analyzed by using the quantitative RT-PCR. As a consequence, 48 clones each from the cDNA libraries were selected as Orobanche-induced genes and Striga-induced genes. Attachment of S. hermonthica specifically induced expression of genes involved in phytoalexin biosynthesis, suggesting that L. japonicas recognizes the incompatible S. hermonthica as an unfavorable intruder. Meanwhile, parasitism of O. aegyptiaca specifically induced expression of genes involved in jasmonic acid. Nodulation-related genes were almost exclusively found in the Orobanche-induced genes. In addition, the temporal changes in the expression level of the Orobanche-induced genes and the Striga-induced genes were evaluated in the L. japonicus roots at 1, 2, 6, and 10 days after inoculation (dai). Forty percent of the Orobanche-induced genes were up-regulated at 1 dai. The prompt response of these genes to parasitism of O. aegyptiaca were similar to that of Lotus japonicus genes to infection of Mesorhizobium loti.
植物化学調節学会, 2008年10月06日, 植物化学調節学会研究発表記録集, (43), 26 - 26, 日本語 - 10.根寄生植物種子発芽刺激活性に対するストライゴラクトンB環修飾の効果(口頭発表)
The seeds of root parasitic plants Orobanche spp. and Striga spp. only germinate when they are exposed to stimulant molecules, collectively referred to as strigolactones. Isolation of orobanchol and its acetate from red clover and cowpea root exudates, respectively, suggest the importance of modification at C-4 of the strigolactone molecule. In the present study we prepared HO-GR24 and AcO-GR24, both of which are modified at C-4 of the GR24 molecule, and evaluated their germination-inducing activity toward seeds of O. minor, S. hermonthica and S. gesnerioides. Modification of GR24 at C-4 with an acetoxy group did not affect the bioactivity toward S. hermonthica and O. minor. However, introduction of a hydroxy group at C-4 increased the activity toward O. minor, but decreased toward S. hermonthica. S. gesnerioides seeds did not respond to any of the synthetic strigolactones although the seeds were responsive to cowpea root exudates. These results demonstrate that modifications at C-4 affect the bioactivity. Moreover, the importance of the A-ring for the bioactivity toward S. gesnerioides seeds was suggested.
植物化学調節学会, 2008年10月06日, 植物化学調節学会研究発表記録集, (43), 24 - 24, 日本語 Parasitic plants cause devastating losses to crop yields in several parts of the world. The root parasites, Striga and Orobanche species, use chemical signalling molecules that are exuded by the roots of plants in extremely low concentrations, and that can induce germination of the seeds of these parasites, to detect the vicinity of a suitable host. The majority of the so far identified germination stimulants belong to the strigolactones. It was recently discovered that this class of compounds can also induce hyphal branching in the symbiotic arbuscular mycorrhizal fungi, a process involved in root colonisation. The elucidation of the structure of new strigolactones is hindered by their low abundance and instability. In the present paper, we have used existing knowledge on the structure of strigolactones and combined it with recently obtained insight in the biosynthetic origin of these signalling compounds. This enabled us to postulate structures for strigolactones that have been isolated but for which so far the structure has not been elucidated, but also to propose structures of strigolactones that may be discovered in the future. Considering the strongly increased importance of the strigolactones, we expect that more groups will look for these compounds and also in systems so far not exploited. This could lead to the discovery of new strigolactones for which we expect the present biogenetic considerations will facilitate identification and structure elucidation. (c) 2008 Elsevier Masson SAS. All rights reserved.
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2008年07月, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46 (7), 617 - 626, 英語[査読有り]
書評論文,書評,文献紹介等
- 日本農薬学会, 2008年03月11日, 日本農薬学会大会講演要旨集, 33, 38 - 38, 日本語根寄生植物の発芽刺激物質ストライゴラクトンの合成と生物活性
- ストライガの接触によるミヤコグサでのファイトアレキシン生合成の誘導
Lotus japonicus, incompatible to Striga hermonthica and compatible to Orobanche aegyptiaca, was employed to study host responses at early stages of parasitization. cDNA libraries enriched by suppression subtractive hybridization for transcripts up-regulated in L. japonicus root segments adjacent to the attachment points of S. hermonthica seedlings (Lj-Sh) and those adjacent to O. aegyptiaca tubercles have been established. Genes involved in the biosynthesis of vestitol, an isoflavan phytoalexin produced by Lotus and other leguminous genera, were exclusively found in Lj-Sh. Expression of genes related to vestitol biosynthesis was higher in the roots attached with S. hermonthica than those parasitized by O. aegyptiaca and non-inoculated control roots. Metabolites exuded from L. japonicus seedlings inoculated with S. hermonthica were collected and a chemical was isolated after separation by analytical HPLC. Chromatographic behavior on HPLC, molecular formula and fragmentation pattern in the EI-MS of the chemical were identical to those of authentic vestitol. Fluorescence similar to that of authentic vestitol was observed on the surface of L. japonicus roots attached with S. hermonthica. It is suggested that the host roots recognize incompatible S. hermonthica as an unfavorable intruder. Vestitol may function as a chemical barrier to prevent S. hermonthica invasion.
植物化学調節学会, 2008年, 植物化学調節学会第43回大会研究発表記録集, 2008, 27, 27 - 27, 日本語 - 根寄生植物-宿主植物間相互作用の分子解析(III) : ストライガの寄生に対して感受性が異なるソルガムの分子応答
Sorghum genes responsive to Striga hermonthica parasitism were isolated and their expression was analyzed. Using the suppression subtractive hybridization (SSH) strategy and the quantitative RT-PCR (qRT-PCR), 30 genes, up-regulated in response to S. hermonthica parasitism, were isolated from roots of a susceptible sorghum cultivar Abu 70. Changes in expression of each of the genes were investigated in roots and leaves obtained from the S. hermonthica-parasitized sorghum cultivars Tabat and Wad Ahmed, more and less susceptible to S. hermonthica than cv. Abu 70, respectively. The changes were studied also in roots of the three sorghum cultivars treated with salicylic acid (SA) or methyl jasmonate (MeJA). S. hermonthica parasitism induced jasmonic acid (JA)-responsive genes and suppressed SA-responsive genes in roots of cvs. Abu 70 and Tabat. In contrast, in the less susceptible cultivar Wad Ahmed, S. hermonthica parasitism induced SA-responsive genes but slightly induced JA-responsive genes. These suggest that the susceptible hosts recognize S. hermonthica parasitism as abiotic stress rather than biotic stress and that the less susceptible host recognize S. hermonthica parasitism as biotic stress. Systemic expression of a few genes was observed in leaves of the sorghum cultivars parasitized with S. hermonthica.
植物化学調節学会, 2007年10月05日, 植物の生長調節 = Regulation of plant growth & development, 42, 78 - 78, 日本語 - 根寄生植物の寄生に対するミヤコグサの応答の分子解析
Lotus japonicus, susceptible to Orobanche aegyptiaca and insusceptible to Orobanche minor, Striga hermonthica and Striga gesnerioides, was employed to study host responses at early stages of parasitization. O. aegyptiaca and O. minor penetrated into L. japonicus root. Although O. aegyptiaca formed tubercle on the root, no tubercle of O. minor was observed. S. hermonthica and S. gesnerioides were failed to penetrate into L. japonicus root. At the attachment point of S. hermonthica to the L. japonicus root, browning of the host tissue was observed. Based on the observation of L. japonicus responses to invasion of different parasitic plants, we selected O. aegyptiaca and S. hermonthica for further work. Genes up-regulated in L. japonicus root segments adjacent to O. aegyptiaca tubercles (LjOa) and adjacent to the attachment points of S. hermonthica seedlings (LjSh) were isolated by suppression subtractive hybridization. Following sequencing and expression analyses, 51 and 38 clones from LjOa and LjSh libraries, respectively, were isolated. They include transcripts predicted to function in stress responses such as enzymes involved in jasmonic acid biosynthesis and detoxification of active oxygen. Genes involved in PR protein were significantly more abundant in the LjOa library compared with the LjSh library. Transcripts associated with nodulation were found exclusively in the LjOa library, while those related to phytoalexin biosynthesis were found exclusively in the LjSh library.
植物化学調節学会, 2007年10月05日, 植物の生長調節 = Regulation of plant growth & development, 42, 79 - 79, 日本語 - 植物化学調節学会, 2006年10月04日, 植物の生長調節 = Regulation of plant growth & development, 41, 67 - 67, 日本語根寄生植物-宿主植物間相互作用の分子解析(II)ストライガの寄生に対する感受性の異なるソルガムの応答
- 植物化学調節学会, 2006年10月04日, 植物の生長調節 = Regulation of plant growth & development, 41, 59 - 59, 日本語高温にさらされたシロイヌナズナでのマロンジアルデヒドの機能解析
- OXFORD UNIV PRESS, 2006年, PLANT AND CELL PHYSIOLOGY, 47, S44 - S44, 英語Biochemical and molecular biological effects of malondialdehyde formed in heat stressed plants
研究発表ペーパー・要旨（国際会議）
- 47. 根寄生植物-宿主植物間相互作用の分子解析(I) : ストライガの寄生に対するソルガムの応答
Striga spp., root parasites of poaceous plants, devastate cereal production in the semiarid Sub Saharan Africa. The life cycle of the parasite is strongly cued to that of its host and is modulated by host-derived chemical signals. To germinate Striga seeds require conditioning in a warm moist environment and exposure to host derived stimulants, collectively named strigolactones. In this study, host-parasite interactions were analyzed at the molecular level. DNA sequences of clones, isolated by a suppression subtractive hybridization strategy from Striga-parasitized sorghum, were homologous to genes for fatty acid desaturase of maize, heat shock protein 82 of rice, calcium dependent protein kinase of maize and sorghum ESTs induced by biotic and abiotic stresses, including wound, pathogen-infection and oxidative stress.
植物化学調節学会, 2005年10月13日, 植物化学調節学会研究発表記録集, (40), 66 - 66, 日本語 - 48. カプセル化による根寄生雑草種子発芽刺激物質の安定化
The genera Striga and Orobanche comprise serious and debilitating obligate root parasitic weeds on several economically important poaceous and leguminous crops. Prodigious seed production, prolonged seed viability and special germination requirements make Striga and Orobanche species difficult weeds to control. To germinate the seeds require a pre-treatment in a warm moist environment and a subsequent exposure to a germination stimulant. In nature, the stimulant is exuded by roots of host and some non-host plants. Natural germination stimulants, strigolactones, were isolated, identified and active structural analogues were prepared. Induction of germination, in absence of host plants, suicidal germination, leads to depletion of the seed reserves in soils. However, the natural stimulants and their structural analogues are extremely labile and proved to be ineffective under practical field conditions. Current research in our laboratory on slow release formulations of GR24, a synthetic germination stimulant, indicates that encapsulation increased persistence and resulted in prolonged activity of the compound. Increased persistence and prolonged activity of the compound make adoption of suicidal germination, as an effective and practical means for controlling Striga and Orobanche under field conditions, an intriguing possibility.
植物化学調節学会, 2005年10月13日, 植物化学調節学会研究発表記録集, (40), 67 - 67, 日本語 - OXFORD UNIV PRESS, 2005年, PLANT AND CELL PHYSIOLOGY, 46, S78 - S78, 英語Peroxidation of linolenic acid triggered by heat and reactive oxygen species causes protein modification in chloroplasts
研究発表ペーパー・要旨（国際会議）
- 2005年, 化学と生物, 43 (8), 538 - 541根寄生植物ストライガの種子発芽戦略
- 日本農薬学会, 2004年03月19日, 講演要旨集, 29, 64 - 64, 日本語ミヤコグサが生産する根寄生雑草ストライガ種子発芽刺激物質の精製
- 51. ワタの生産する根寄生雑草発芽刺激物質
Seed germination of Orobanche spp. and Striga spp. is triggered by germination stimulants (strigolactones) released from roots of host plants. We have established an LC/MS/MS method for determination and quantification of strigolactones (Sato et al., 2003). This method enables direct analysis of strigolactones in crude ethyl acetate extracts of root exudates without any purification. By using this LC/MS/MS method, we identified and quantified strigolactones in root exudates of several plant species. In this study, we aimed to clarify strigolactones produced by cotton. In our experiment, cotton was confirmed to produce strigol and strigyl acetate but not orobanchol or alectrol. Time-course of strigol production by cotton seedlings was examined and higher strigol production was observed for 5-7 days after incubation. Cotton seedlings were found to produce 30 pg/day/plant of strigol and 3 pg/day/plant of strigyl acetate. Strigyl acetate was only 1/100 as active as strigol on O. minor seed germination. Characterization of strigolactones produced by other host plants is in progress.
植物化学調節学会, 2003年10月10日, 植物化学調節学会研究発表記録集, (38), 68 - 68, 日本語 - 日本作物学会, 2003年04月04日, 日本作物學會紀事, 72 (1), 248 - 249, 日本語ソルガムの水ストレス耐性に対するケイ酸の施用効果
- 日本農薬学会, 2003年03月14日, 講演要旨集, 28, 49 - 49, 日本語根寄生雑草 Striga hermonthica の発芽戦略におけるエチレンの役割
- 日本農薬学会, 2003年03月14日, 講演要旨集, 28, 50 - 50, 日本語根寄生植物種子発芽刺激物質の植物界における分布 : Part 1
- 2003年, 根の研究, 12 (2), 51 - 56根寄生植物の種子発芽を刺激する物質
- 64 LC/MS/MS によるストリゴラクトン類(寄生雑草発芽刺激物質)の同定・定量
Witchweeds (Striga spp.) and broomrapes (Orobanche spp.) are two most devastating root parasitic weeds causing sever damage to agricultural production. Seeds of Striga and Orobanche germinate only when they receive chemical signals, i.e., germination stimulants, produced by and released from roots of host or non-host plants. So far, four different germination stimulants (Fig. 1), strigol (1), sorgolactone (2), alectrol (3) and orobanchol (4), collectively called strigolactones, have been isolated and characterized. These strigolactones induce significant germination of Striga and Orobanche seeds at as low as 10^<-10> M and thus the amounts of strigolactones in root exudates have been estimated by bioassays. However, root exudates may contain germination inhibitors as well and thus the amounts of strigolactones might have been underestimated. Therefore, we developed a rapid and sensitive analytical method for strigolactones by high performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). In this study, three authentic strigolactones were separated clearly on MRM (Fig. 2). Using this method, we confirmed that red clover (Trifolium pratense cv. Hamidori), a host of O. minor, produces orobanchol and alectrol but not strigol nor sorgolactone (Fig. 3). Each red clover seedling produced ca. 13,70,58,and 65 pg of orobanchol for the 1st, 2nd, 3rd and 4th weeks after germination.
植物化学調節学会, 2002年10月31日, 植物化学調節学会研究発表記録集, (37), 137 - 138, 日本語 - 63 ANALYSIS OF ETHYLENE BIOSYNTHESIS GENES IN OROBANCHE MINOR
Although the seed germination of root hemiparasites, Striga spp., is induced by ethylene, there are controversial reports concerning the role of ethylene in the seed germination of root holoparasites, Orobanche spp. Therefore, two genes involved in ethylene production (ACC synthase and ACC oxidase) were cloned from O. minor and sequenced. The partial sequences obtained so far demonstrated that O. minor ACC synthase may not be functional or under control of a unique regulatory system. By contrast, O. minor ACC oxidase had high homologies with those in other plant species.
植物化学調節学会, 2002年10月31日, 植物化学調節学会研究発表記録集, (37), 135 - 135, 英語 - 67 STRIGOLACTONES, GERMINATION STIMULANTS FOR ROOT PARASITES, PRODUCED BY COTTON AND SORGHUM
Seed germination of root parasitic weeds, Striga spp. and Orobanche spp., is elicited by exogenous stimulants released from roots of host and nonhost plants. Strigol was first isolated from a false host, cotton (Gossypium hirsutum L.) as a germination stimulant for S. asiatica and later also shown to be a major stimulant in root exudates of a host of S. hermonthica, sorghum (Sorghum bicolor (L.) Moench). Sorghum root exudates were reported to contain other stimulants including sorgolactone. Since trace levels of these known germination stimulants (strigolactones) can be detected by HPLC-tandem mass spectrometry (LC/MS/MS), root exudates of cotton and sorghum were analyzed with this method. It was confirmed that cotton (G. hirsutum cv. Fibre Max) produce strigol but not alectrol nor orobanchol. Although the major stimulant in sorghum root exudates has been identified as strigol with CI-MS of the heptafluorobutyrate derivative, it appears to be a novel strigol isomer, not strigol itself. The sorghum root exudates also contained sorgolactone as a minor stimulant.[table]
植物化学調節学会, 2002年10月31日, 植物化学調節学会研究発表記録集, (37), 143 - 144, 英語 - 日本雑草学会, 2002年04月13日, 雑草研究. 別号, 講演会講演要旨, (41), 144 - 145, 日本語64 寄生雑草(Orobanche minor, O. ramosa, Striga asiatica, S. hermonthica)種子の発芽に及ぼすfluridoneの影響
- 日本農薬学会, 2002年02月22日, 講演要旨集, 27, 53 - 53, 日本語根寄生雑草発芽刺激物質のLC/MS/MSによる微量分析
- 50 非宿主植物コウモリカズラ培養根からの根寄生雑草の発芽刺激物質strigolの単離
Root culture of Menispermum dauricum exudes a germination stimulant for root parasitic weeds. Active ingredient was extracted from the culture filtrate and purified by sephadex LH-20 column chromatography and preparative HPLC. On the basis of physicochemical parameters, the germination stimulant was identified as (+)-strigol. Ten liter culture filrate yielded ca. 38 μg strigol.
植物化学調節学会, 2001年10月09日, 植物化学調節学会研究発表記録集, (36), 111 - 111, 日本語 - 51 寄生雑草Striga asiaticaの種子発芽に対するフルリドン促進効果
Fluridone added to the incubation media during conditioning induced seed germination of Slriga asiayica in the absence of a germination stimulant, strigol in the fiuridone-treated seeds, most of the radicles did not elongate and looked like haustria. Furthermore, fiuridione treatment increased sensitivity of the seeds to strigoi. Norfiurazon, another inhibitor of carotenoid biosynthesis, also increased the sensitivity but no germination occurred in the absence of strigoi. By contrast, uniconazole strongly inhibited these processes.
植物化学調節学会, 2001年10月09日, 植物化学調節学会研究発表記録集, (36), 113 - 114, 日本語 - 2001年04月14日, 雑草研究, 46, 118 - 119, 日本語ブラシナゾール誘導体のヤセウツボ(Orobanche. minor)種子のコンディショニングにおよぼす影響
- 2001年, Proc. 7th Intl. Parasitic Weed Symposium, 106 - 109, 英語Identificationof ACC synthase and ACC oxidase genes in germination Striga hermonthica seeds.
研究発表ペーパー・要旨（全国大会，その他学術会議）
- 植物化学調節学会, 2000年12月25日, 植物の化学調節, 35 (2), 225 - 226, 日本語17. Fusarium solaniの生産する根寄生雑草Striga hermonthicaの発芽阻害物質(第35回大会研究発表抄録)
- 植物化学調節学会, 2000年12月25日, 植物の化学調節, 35 (2), 233 - 234, 日本語42. ヤセウツボ(Orobanche minor)種子の発芽応答と内生ABAの関係(第35回大会研究発表抄録)
- 17 Fusarium solaniの生産する根寄生雑草Striga hermonthicaの発芽阻害物質
Fusarium solani (Sud96) isolated from wilted Striga hermonthica plants inhibited Striga seed germination induced by the germination stimulant GR24. The fungus extracts were separated by silica gel chromatography. According to bioassay, 4 fractions were found to have high inhibitory activity. Further purification was undertaken and identification of active principles is in progress using spectroscopic and chemical methods.
植物化学調節学会, 2000年11月02日, 植物化学調節学会研究発表記録集, (35), 41 - 41, 日本語 - 42 ヤセウツボ(Orobanche minor)種子の発芽応答と内生ABAの関係
Seeds of Orobanche minor Sm., a root parasite, have special germination requirements, including pretreatment (conditioning in a warm moist environments for several days prior to exposure to exogenous germination stimulants. Temperature has been shown to affect germination response of the parasite seeds. In particular, when the seeds were exposed to supraoptimal temperatures (over 30℃) during conditioning period, germination induced by dl-strigol decreased dramatically. This inhibition by supraoptimal temperatures could be partially recovered by adding an inhibition of ABA biosynthesis, fluridone, or GA_3 to the incubation media. However, there was no clear relationships between endgenous ABA levels quantified by LC/MS/MS and germination response. Therefore, endogenous GAs seem to play an important role in seed germination of the parasite.
植物化学調節学会, 2000年11月02日, 植物化学調節学会研究発表記録集, (35), 91 - 91, 日本語 - 2000年04月20日, 雑草研究, 45, 74 - 75, 日本語ヤセウツボ(Orobanche minor Smith)種子のコンディショニングにたいする高温障害の回避
- 2000年04月20日, 雑草研究, 45, 72 - 73, 日本語ヤセウツボ(Orobanche minor Smith)種子のコンディショニングにたいする高温障害
- 日本農薬学会, 2000年03月01日, 講演要旨集, 25, 28 - 28, 日本語根寄生雑草の発芽制御物質
- 日本農薬学会, 2000年, 日本農薬学会誌, 25 (4), 438 - 440, 日本語根寄生雑草の発芽刺激物質(最近の農薬的天然生理活性物質に関する新展開)
- 植物化学調節学会, 1999年12月25日, 植物の化学調節, 34 (2), 359 - 360, 日本語87. 根寄生雑草Striga hermonthicaの発芽応答性遺伝子の解析
- 植物化学調節学会, 1999年12月25日, 植物の化学調節, 34 (2), 360 - 360, 日本語88. Fusarium solaniの生産する根寄生性雑草発芽刺激物質
- 植物化学調節学会, 1999年12月25日, 植物の化学調節, 34 (2), 181 - 190, 日本語根寄生雑草Striga類およびOrobanche類における種子発芽の化学的誘導
- 88 Fusarium solani の生産する根寄生性雑草発芽刺激物質
In the course of our screening for search germination stimulants for Striga hermonthica, one of notorious weedy root parasites, Fusarium solani f. radicicola was found to produce such active compounds, but the amount of the stimulants produced by the fungus was too small to be isolated. Attemps to improve its production showed that Czapek medium using sucrose as carbon source is a good medium for the production.
植物化学調節学会, 1999年10月01日, 植物化学調節学会研究発表記録集, (34), 169 - 170, 日本語 - 87 根寄生雑草 Striga hermonthica の発芽応答性遺伝子の解析
Striga hermonthica (Del.)Benth. is a root parasitic weed that reduces the yield of important cereals in semi-arid Sub Saharan Africa. Germination of the parasite seeds, induced by a synthetic germination stimulant GR24, was inhibited by AVG, an inhibitor of ACC synthase activity, and 1-MCP, a competitive inhibitor of ethylene receptor. Inhibition by AVG was restored by ACC, the immediate ethylene precusor, whereas that by 1-MCP was not. These results suggest that ethylene biosynthesis is involved in S.hermonthica germination. RNAs, isolated from GR24- or water-treated seeds, were used for PCR-based amplification of DNA fragments encoding the ACC synthase-active site domain. Two ACC synthas genes named SH-ACS1 and SH-ACS2 were obtained. Both genes were expressed irrespective of the treatment. However, northern blot analysis showed that expression of SH-ACS1 exhibited a temporal change, after GR24 treatment, which was in accord with that of ethylene evolution from the seeds. Consequently, it is concluded that SH-ACS1 gene plays a crucial role in regulating germination of S.hermonthica seeds.
植物化学調節学会, 1999年10月01日, 植物化学調節学会研究発表記録集, (34), 167 - 168, 日本語 - 植物化学調節学会, 1999年06月30日, 植物の化学調節, 34 (1), 116 - 118, 日本語スーダンの農業事情と寄生雑草による被害状況
- 日本化学会, 1999年05月, 化学と工業 = Chemistry and chemical industry, 52 (5), 598 - 601, 日本語半乾燥地における食糧増産に向けて-根寄生雑草ストライガの発芽制御-
- A-4 根寄生雑草 Striga hermonthica の種子発芽に対するエチレンの関与
Experiments were undertaken to verify the involvement of ethylene in germination of the hemiparaitic angiosperm Striga hermonthica (Del.) Benth. Germination was inhibited by AVG (aminoethoxyvinyl glycine), an inhibitor of ACC synthase activity, and restored by ACC (1-aminocyclopropane-1-carboxylic asid), the immediate ethylene precursor. Ethylene release from GR24-treated seeds commenced 6 h after treatment, while germination was first visible 12 h later. These results strongly suggest involvement of ethylene is S. hermonthica germination. RNAs, isolated from GR24-treated seeds, were used for PCR-based amplification of DNA fragments encoding the ACC synthase-active site domain. Three DNA fragments, indicating involvement of 3 different ACC synthase genes, were identified.
植物化学調節学会, 1998年10月01日, 植物化学調節学会研究発表記録集, (33), 17 - 18, 日本語 - B-21 Fusicoccane化合物の寄生雑草種子発芽刺激活性における構造要求性
Fusicoccanes including fusicoccins (FCs), cotylenins (CNs), ophiobolines, and their synthetic analogs were examined for their effects on seed germination of important root parasitic weeds, witch-weed [Striga hermonthica (Del.) Benth] and clover broomrape [Orobanche minor Smith]. Since FCs and CNs carrying different sugar moieties showed similar levels of stimulative activity and, in addition, aglycones were more active than the corresponding parent compounds, aglycones appeared to contain the essential structure for stimulative activity. Furthermore, 9-deoxy-19-hydroxycotylenol was the most active among the compounds tested, indicating that the substituent on the 9-position is not needed for the acitivity. By contrast, the presence of hydroxy group either on the 3- or 12- position seemed to be essential for the activity.
植物化学調節学会, 1998年10月01日, 植物化学調節学会研究発表記録集, (33), 50 - 50, 日本語 - 日本作物学会, 1998年04月02日, 日本作物學會紀事, 67, 62 - 63, 英語根温に対する茎内水分輸送の反応
- 日本作物学会, 1998年04月02日, 日本作物學會紀事, 67, 218 - 219, 日本語ダイズ幼根の伸長に対するNaの阻害はどのようにCaによって軽減されるか
- 日本作物学会, 1998年04月02日, 日本作物學會紀事, 67, 220 - 221, 英語イネ種子根の内皮におけるケイ素の分布
- 1998年03月30日, 雑草研究, 43, 276 - 277, 日本語ジャスモン酸類による寄生雑草種子の発芽誘導
- 植物化学調節学会, 1997年11月28日, 植物化学調節学会研究発表記録集, (32), 59 - 59, 日本語27 コチレニン類による寄生雑草(Striga hermonthica, Orobanche minor)の種子発芽誘導
- 植物化学調節学会, 1997年11月28日, 植物化学調節学会研究発表記録集, (32), 57 - 57, 日本語26 ジャスモン酸誘導体の寄生雑草(Striga hermonthica, Orobanche minor)種子に対する発芽誘導作用
- 日本作物学会, 1996年04月03日, 日本作物學會紀事, 65 (1), 212 - 213, 日本語106 培地の水ポテンシャルの低下がイネ科作物3種の根の成長に及ぼす影響
- 94 イネ科作物4種における土壌乾燥に対する生長反応の種間差
4作物の耐乾性の差異は主に低土壌水分に対する根系の生長反応の違いに基づく.
日本作物学会, 1995年04月03日, 日本作物學會紀事, 64 (1), 188 - 189, 日本語 - 103 AE法による植物根系成長の非破壊計測システムの開発
AE法を用い, 砂培地での植物の根系成長を経時的, 非破壊的かつ3次元的に位置標定することを可能とした.
日本作物学会, 1994年04月01日, 日本作物學會紀事, 63 (1), 206 - 207, 日本語 - 62 ナタネの種子における水分含有量, 無機成分含有量およびアブシジン酸濃度の発育に伴う変化
種子の物質蓄積の停止には含水率の低下, ABA濃度の変化および種皮でのCaの蓄積が密接に関連していることが明らかとなった.
日本作物学会, 1993年10月18日, 日本作物學會紀事, 62 (2), 123 - 124, 日本語 - 日本作物学会, 1993年04月01日, 日本作物學會紀事, 62 (1), 206 - 207, 日本語103 "葉ざし"法によるトウモロコシー次根の伸長速度に及ぼす低水ポテンシャルの影響
- 日本作物学会, 1993年, 日作紀, 62 (1), 204 - 205, 日本語土壌水分低下に対する反応の作物種間差 -ダイズ, ラッカセイ, キビの比較-
書籍等出版物
- Methods in Molecular Biology series, Springer Nature, 2021年Isolation and identification of naturally occurring strigolactones. (Eds) Cristina Prandi and Francesca Cardinale, Strigolactones,
- 106-110, 植物病理学 第二版, 2020年03月眞山滋志、難波成任編 植物病理学 第二版 文永堂
- 文永堂, 2020年03月第2章 寄生性高等植物
- 共著, Springer Verlag, 2018年08月, 英語Improvement of food security in semiarid regions of Sudan through management of root parasitic weeds
学術書
- 共著, 関東雑草研究会報, 2017年02月, 日本語根寄生雑草の生存戦略の化学的解明と防除への応用
一般書・啓蒙書
- 48-54, Daya Publication House, 2010年Search for useful substances in papyrus Cyperus papyrus L. In Chapter 3, Utilisation and Management of Medicinal Plants
- 共著, 文永堂, 2010年, 日本語, 第3章病原体の種類と分類 寄生性高等植物植物病理学
学術書
- 共著, Nova Publishers, 2008年, 英語, Biosynthesis of chlorinated alklaoids in Menispermum dauricum root culturePhytochemistry Research Progress
学術書
- 共著, Springer,, 2008年, 英語, Induction of novel metabolites by p-450 inhibitors in cultured roots of Stephania cepharantha and Menispermum dauricumMedicinal Plants
学術書
- 単著, Springer,, 2002年, 英語, In vitro culture and the production of secondary metabolites in Stephania, Biotechnology in Agriculture and ForestryMedicinal and Aromatic Plants XII
学術書
- 単著, Springer,, 1999年, 英語, Menispermum species (moonseed vines): In vitro culture, and the production of dauricine and other secondary metabolitesMedicinal and Aromatic Plants XI
学術書
- 単著, 朝倉書店, 1998年, 日本語, 根における２次代謝産物の産生根の事典
学術書
- 単著, 講談社サイエンティフィク, 1992年, 日本語, 根の器官培養によるアルカロイド生産植物分子細胞工学マニュアル
学術書
講演・口頭発表等
- 第13回熱帯バイオマスフラッグシップシンポジウム（第488回生存研シンポジウム）、京大生存圏研究所主催オンライン, 2022年12月根寄生雑草の防除による食糧増産
- 植物化学調節学会, 2022年11月, 日本語ヤセウツボのα-ガラクトシダーゼに対する阻害剤のスクリーニングと発芽への影響解析
- 植物化学調節学会, 2022年11月, 日本語ヤセウツボ幼根伸長阻害効果を示す芳香族カルボニルチオウレアの構造活性相関
- 植物化学調節学会, 2022年11月, 日本語トマトにおいて18-oxo-CLAを基質とするorobanchol合成酵素の同定
- 植物化学調節学会, 2022年11月, 日本語OPRによるストリゴラクトンD環の立体選択的還元
- 植物化学調節学会, 2022年11月, 日本語セイヨウチャヒキが生産する6-epi-heliolactoneの単離・同定とavenaol生合成経路の解析
- 植物化学調節学会, 2022年11月, 日本語トマトが生産する新規ストリゴラクトンの同定
- 植物化学調節学会, 2022年09月, 日本語ヤセウツボのα-ガラクトシダーゼに対する阻害剤のスクリーニングと発芽への影響解析
- 植物化学調節学会, 2022年09月, 日本語Gossypium hirsutumにおけるstrigol合成酵素の同定
- 日本植物細胞分子生物学会, 2022年09月オオムギが生産するストリゴラクトンの構造解析
- 日本植物細胞分子生物学会, 2022年09月, 日本語GCaMP3を用いたシロイヌナズナの匂い受容シグナル情報伝達系の解析
- 日本植物細胞分子生物学会, 2022年09月, 日本語ジャガイモのα-ソラニン生合成に関わるソラニダン還元酵素の解析
- 日本植物細胞分子生物学会, 2022年09月ゼニゴケにおけるビタミンD3高生産を目指した代謝工学
- 日本植物細胞分子生物学会, 2022年09月, 日本語ゼニゴケにおけるトリテルペノイド高生産系の構築
- 16th World Congress on Parasitic Plants, Nairobi,, 2022年07月, 英語Strucutre-activity relationship of aromatic carbonyl thioureas for inhibition of Orobanche minor radicle elongation
- 日本農芸化学会, 2022年03月ワタ（Gossypium hirsutum）におけるstrigol合成酵素の探索
- 日本農芸化学会, 2022年03月遺伝子組み換え植物による活性型ビタミンD3の合成
- 日本農芸化学会, 2022年03月ステロイドグリコアルカロイド生合成に関わる新規生合成遺伝子の機能解析
- 日本農芸化学会, 2022年03月SlCYP722C による 18-oxo-CLA の生成と非酵素的反応による orobanchol の BC 環形成
- 日本農芸化学会, 2022年03月セイヨウチャヒキにおけるavenaol生合成前駆体としての6-epi-heliolactoneの同定
- 日本農芸化学会, 2022年03月ジャガイモシストセンチュウふ化促進物質の活性を増強する共力因子の解析
- 日本農芸化学会, 2022年03月ジャガイモ水耕液からのジャガイモシストセンチュウに対する新規ふ化促進物質の同定
- 植物化学調節学会, 2021年11月シロイヌナズナにおける CYP722A の機能解析
- 植物化学調節学会, 2021年11月ヒマワリにおける非典型的ストリゴラクトンの生合成遺伝子の探索
- 植物化学調節学会, 2021年11月ジャガイモの毒α-ソラニンはトマトの苦味成分スピロソランから分子進化した
- 植物化学調節学会, 2021年11月寄生雑草ストライガのアブシシン酸シグナル伝達因子脱リン酸化酵素遺伝子の機能解析
- 植物化学調節学会, 2021年11月SlCYP722C による 18-oxo-CLA の生成と非酵素的反応による orobanchol の BC 環形成
- 3rd International Strigolactone Congress, China, 2021年10月BC-ring formation in canonical strigolactones
[招待有り]
- University of Khartoum, Sudan, 2021年10月06日Introductory mote on the project (Invited), National Seminar on Integrated Management of Parasitic Weeds,
- 第65回 香料・テルペンおよび精油化学に関する討論会, 2021年10月ストリゴラクトンのBC環形成過程の解明を志向したオロバンコールの合成化学的研究
- 日本農薬学会第46回大会シンポジウム「生物と化学のはざまで」, 2021年03月10日ストリゴラクトンの立体と構造の多様性
- 農薬学会第 46 回大会, 2021年03月, 日本語根寄生雑草ヤセウツボの発芽におけるプランテオースの代謝部位
- 日本農芸化学会, 2021年03月ヤセウツボ種子内においてプランテオースを加水分解するα-ガラクトシダーゼの局在の解明
- 日本農芸化学会, 2021年03月, 日本語推定生合成経路に基づくストリゴラクトン類の合成研究
口頭発表（一般）
- 植物化学調節学会, 2020年11月, 日本語ミヤコグサにおけるCYP722Cサブファミリーの機能解析
- 第64回 香料・テルペンおよび精油化学に関する討論会, 2020年10月, 日本語カーラクトン酸類の短工程合成法の開発とその応用
- 天然有機化合物討論会, 2020年09月カーラクトン酸類の短工程合成法の開発とその応用によるストリゴラクトン類の合成研究
- 第72回日本生物工学会大会シンポジウム, 2020年09月, 日本語ストリゴラクトン生合成と作物生産への応用
- 日本農芸化学会, 2020年03月カーラクトン酸をオロバンコールへと変換する新規オロバンコール合成酵素の同定
- 植物細胞分子生物学会, 2020年03月トマト毛状根培養系を用いたジャガイモシストセンチュウ孵化促進物質の生合成遺伝子の探索
- 日本農芸化学会関西支部例会, 2020年02月植物の免疫応答に関わるExtra-Large G-protein (XLG)の機能解析
- 日本農芸化学会関西支部例会, 2019年12月トマトにおけるキャリステジン生合成経路の解析
- 日本農芸化学会関西支部例会, 2019年12月ヒマワリにおけるヘリオラクトン生合成機構の解明
- 植物化学調節学会, 2019年11月, 日本語, 国内会議ヤセウツボ発芽種子のトランスクリプトーム解析
- 2019年11月, 日本語, 国内会議ストリゴラクトンのヤセウツボとハマウツボ種子に対する構造要求性の違い
- 植物化学調節学会, 2019年11月, 日本語, 国内会議ヒマワリにおける非典型的ストリゴラクトン生合成経路の解明
- 植物化学調節学会, 2019年11月, 日本語, 国内会議ミヤコグサにおける5-deoxystrigol生合成経路の解明
- 植物化学調節学会, 2019年11月, 日本語, 国内会議ワタにおける5-ストリゴールおよびソルゴモール生合成酵素の探索
- 植物化学調節学会, 2019年11月, 日本語, 国内会議カーラクトン酸をオロバンコールへと変換する新規オロバンコール合成酵素の同定
- 植物化学調節学会, 2019年11月, 日本語, 国内会議寄生植物ストライガのアブシシン酸シグナル伝達因子の機能解析
- 植物化学調節学会, 2019年11月, 日本語, 国内会議トマト毛状根からのジャガイモシストセンチュウ孵化促進物質の探索
- 植物化学調節学会, 2019年11月, 日本語, 国内会議トマトにおけるジャガイモシストセンチュウ孵化促進物質の生合成遺伝子の探索
- 日本植物細胞分子生物学会, 2019年09月, 日本語, 国内会議トマトにおけるキャリステジン生合成経路の解明
- 日本植物細胞分子生物学会, 2019年09月, 日本語, 国内会議ステロール7位還元酵素の遺伝子破壊によるビタミンD3高生産トマトの作出
- 日本植物細胞分子生物学会, 2019年09月, 日本語, 国内会議ジャガイモのソラニン生合成におけるソラニダン骨格形成機構の解析
- 日本植物細胞分子生物学会, 2019年09月, 日本語, 国内会議ジャガイモシストセンチュウ孵化促進物質の生合成に関与する酸化酵素の探索
- 日本植物細胞分子生物学会, 2019年09月, 日本語, 国内会議ヤセウツボのプランテオース代謝に関わるα-ガラクトシダーゼの解析
- TERPENET2019, Halle, Germany, 2019年09月, 英語, 国際会議Characterization of steroid 5-reductase involved in -tomatine biosynthesis in tomato
- TERPENET2019, Halle, Germany, 2019年09月, 英語Analysis of hatching stimulation activity for potato cyst nematodes by steroidal glycoalkaloids.
- 15th World Congress on Parasitic Plants, Amsterdam,, 2019年07月, 英語, 国際会議Identification and characterization of α-galactosidase capable of hydrolyzing planteose in Orobanche minor as a target for control of root parasitic weeds
- 15th World Congress on Parasitic Plants, Amsterdam,, 2019年07月, 英語, 国際会議Aberrant protein phosphatase 2C leads to ABA insensitivity, high transpiration rate and sustenance of parasitism in Striga hermonthica.
- IPGSA, Paris, 2019年06月, 英語, 国際会議Identification and characterization of sorgomol synthase in sorghum
- The 14th IUPAC International Congress of Crop Protection Chemistry, Gent, Belgium, 2019年05月, 英語, 国際会議SCREENING OF GROWTH INHIBITORS OF ROOT PARASITIC WEEDS TARGETING PLANTEOSE METABOLISM
- 日本作物学会, 2019年03月, 日本語接触前および接触後抵抗性に基づく世界のイネコアコレクションのストライガ抵抗性
- 日本農芸化学会, 2019年03月, 日本語Heliolactoneの合成研究
- 2019年03月, 日本語ソルガムのストリゴラクトン生合成経路におけるソルゴモール合成酵素の同定
- 植物生理学会, 2019年03月, 日本語根寄生植物ストライガは機能変異を起こしたProtein Phosphatase 2CによってABA非感受性と高蒸散を示す
- 日本農薬学会, 2019年03月, 日本語根寄生雑草の新規防除標的プランテオース加水分解酵素の諸性質
- 日本農芸化学会 ２０１９年度大会, 2019年03月, 日本語, 東京, 国内会議カーラクトン酸の合成研究
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2018年12月, 日本語酸化ストレス応答に関わる2-hexenal受容体のスクリーニング
- 日本農芸化学会関西支部例会, 2018年12月, 日本語毛状根培養系を利用したジャガイモシストセンチュウ孵化促進物質の探索
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議根寄生植物ストライガのアブシジン酸情報伝達異常の解析
ポスター発表
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議ワタにおける5DS水酸化酵素の探索
ポスター発表
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議シロイヌナズナの病害応答に関わる 3-ヘキセニルアセテートの生理機能の解析
ポスター発表
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議ジャガイモ毛状根培養液からのジャガイモシストセンチュウ孵化促進物質の探索
口頭発表（一般）
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議ササゲとトマトにおけるオロバンコール合成酵素の探索
ポスター発表
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議グリコアルカロイドのジャガイモシストセンチュウ孵化促進活性の解析
ポスター発表
- 第53回植物化学調節学会, 2018年11月, 日本語, 北海道, 国内会議Identification of sorgomol synthase in sorghum
ポスター発表
- The 15th Solanaceae Conference, 2018年10月, 英語, 国際会議Identification of a 3β-hydroxysteroid dehydrogenase/3-ketosteroid reductase involved in α-tomatine biosynthesis in tomato
ポスター発表
- The 15th Solanaceae Conference, 2018年10月, 英語, 国際会議Genome editing of tomato oxidosqualene cyclase genes using CRISPR/Cas9 system
ポスター発表
- 第36 回日本植物細胞分子生物学会（金沢）大会, 2018年08月, 日本語, 国内会議トマトのオキシドスクアレン環化酵素の遺伝子破壊
ポスター発表
- 第36 回日本植物細胞分子生物学会（金沢）大会, 2018年08月, 日本語, 国内会議トマトにおけるαｰトマチン生合成に関わる5位還元酵素の解析
ポスター発表
- 第36 回日本植物細胞分子生物学会（金沢）大会, 2018年08月, 日本語, 国内会議ジャガイモのα_ ソラニン生合成遺伝子と共発現する還元酵素の解析
口頭発表（一般）
- 日本農芸化学会, 2018年03月, 日本語, 国内会議栽培種および野生種トマトにおけるα-トマチン代謝酵素遺伝子の機能解析
口頭発表（一般）
- 日本農芸化学会, 2018年03月, 日本語, 名城大学, 国内会議ヤセウツボのα-ガラクトシダーゼ阻害剤が発芽に与える影響
口頭発表（一般）
- 植物生理学会, 2018年03月, 日本語, 国内会議ハマウツボ科根寄生植物の発芽におけるプランテオース代謝
口頭発表（一般）
- 日本農芸化学会, 2018年03月, 日本語, 名城大学, 国内会議ジャガイモのステロイドグリコアルカロイド生合成に関わる糖転移酵素の機能解析
口頭発表（一般）
- 日本作物学会, 2018年03月, 日本語, 国内会議コアコレクションとライゾトロン法を用いたイネ品種のストライガ接触後抵抗性の評価
口頭発表（一般）
- 日本農芸化学会関西支部例会(第502回講演会), 2018年02月, 日本語, 京都大学百周年時計台記念館 2階 国際交流ホールI, 国内会議シロイヌナズナの病害抵抗性を誘導する3-ヘキセニルアセテートの作用機構の解明
口頭発表（一般）
- SATREPS symposium on Striga management, 2018年, 英語, Khartoum, Sudan, 国際会議Literature survey on the management of root parasitic weeds
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2017年12月, 日本語, 神戸大学, 国内会議シロイヌナズナの2-hexenal情報伝達系に関わるCyclic nucleotide-gated Ca channelのスクリーニング
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 鹿児島大学, 国内会議合成ストリゴラクトンGR24のD環還元を行う酵素の同定
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 国内会議ヤマノイモ属ステロイドサポニン生合成に関わる配糖化酵素の同定および機能解析
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 鹿児島大学, 国内会議ミヤコグサを用いたソルガム由来ソルゴモール合成酵素の機能解析
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 鹿児島大学, 国内会議プランテオース代謝を標的としたヤセウツボ発芽阻害剤のスクリーニング
口頭発表（一般）
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 鹿児島大学, 国内会議ダイズシストセンチュウ孵化促進物質グリシノエクレピンAの検出分析法の確立
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 国内会議ストライガの生産するABAがソルガムの気孔閉鎖と生長阻害を引き起こす
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 国内会議ストライガにおけるストリゴラクトン生合成能の解析
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 鹿児島大学, 国内会議シロイヌナズナの病原菌防御応答における3-へキセニルアセテートの生理機能の解明
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 鹿児島大学, 国内会議シロイヌナズナの酸化ストレス応答に関わるGSTの生化学的・生理学的解析
ポスター発表
- 植物化学調節学会第52回大会, 2017年10月, 日本語, 国内会議シロイヌナズナの環境ストレス応答に関わる2-hexenal 情報伝達機構の解析
ポスター発表
- 日本生物工学会大会, 2017年09月, 日本語, 国内会議根寄生雑草防除のための貯蔵糖プランテオース代謝に関わる酵素の探索
口頭発表（一般）
- 日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会, 2017年09月, 日本語, 大阪府立大学, 国内会議ヤセウツボの発芽に関わる α-ガラクトシダーゼを標的とする阻害剤 の探索
口頭発表（一般）
- 日本農芸化学会 関西・中四国・西日本支部 2017年度合同大会, 2017年09月, 日本語, 大阪府立大学, 国内会議シロイヌナズナの7TMおよびカルシウムチャネル欠損株を用いた2-hexenal 情報伝達系の解析
口頭発表（一般）
- 第35回日本植物細胞分子生物学会, 2017年08月, 日本語, 大宮ソニックシティ(埼玉), 国内会議野生種トマトのステロイドグリコアルカロイドの多様性を担う酵素遺伝子の機能解析
口頭発表（一般）
- 第35回日本植物細胞分子生物学会, 2017年08月, 日本語, 大宮ソニックシティ(埼玉), 国内会議大腸菌発現系を用いた植物オキシドスクアレン環化酵素の機能解析
ポスター発表
- 第35回日本植物細胞分子生物学会, 2017年08月, 日本語, 大宮ソニックシティ(埼玉), 国内会議ヤマノイモ属トゲドコロのステロイドサポニン生合成に関わる糖転移酵素の機能解析
ポスター発表
- 第35回日本植物細胞分子生物学会, 2017年08月, 日本語, 大宮ソニックシティ(埼玉), 国内会議ヤセウツボの種子発芽におけるプランテオース代謝に関わる酵素の探索
口頭発表（一般）
- 第35回日本植物細胞分子生物学会, 2017年08月, 日本語, 大宮ソニックシティ(埼玉), 国際会議ジャガイモα-ソラニン生合成遺伝子St16DOXのゲノム編集
ポスター発表
- The 13th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids (TERPNET 2017), 2017年07月, 英語, Dalian International Conference Center, China, 国際会議Genome editing of α-solanine biosynthetic gene in potato
口頭発表（一般）
- ICBM2017, 2017年07月, 英語, Dalian, 国際会議Carbohydrate Metabolism during Seed Germination of Orobanche minor as a Target for Selective Control of Root Parasitic Weeds
口頭発表（一般）
- 日本農芸化学会関西支部 第499回講演会, 2017年06月, 日本語, 国内会議酸化ストレス応答性シロイヌナズナGSTの解析
口頭発表（一般）
- 14th WORLD CONGRESS ON PARASITIC PLANTS, 2017年06月, 英語, ASIOMAR CONFERENCE GROUNDS, 国際会議STOMATAL CLOSURE AND GERMINATION IN STRIGA HERMONTHICA ARE NOT SENSITIVE TO ABSCISIC ACID
口頭発表（一般）
- 14th WORLD CONGRESS ON PARASITIC PLANTS, 2017年06月, 英語, ASIOMAR CONFERENCE GROUNDS, 国際会議Practicality of the Suicidal Germination Approach for Controlling Striga hermonthica in Sudan
ポスター発表
- 日本農薬学会, 2017年03月, 日本語, 国内会議根寄生雑草ヤセウツボの発芽種子で発現している α-ガラクトシダーゼの機能解析
口頭発表（一般）
- 日本農芸化学会2017年度大会, 2017年03月, 日本語, 京都女子大学, 国内会議根寄生雑草の種子発芽刺激物質の化学と自殺発芽誘導への応用
口頭発表（一般）
- 日本農芸化学会2017年度大会, 2017年03月, 日本語, 京都女子大学, 国内会議ヤマノイモ属におけるステロイドサポニン生合成の配糖化段階に関わる糖転移酵素の探索
口頭発表（一般）
- 日本農芸化学会, 2017年03月, 日本語, 国内会議ヤセウツボの種子発芽に特徴的なプランテオース代謝に関わる酵素の探索
口頭発表（一般）
- 日本農芸化学会2017年度大会, 2017年03月, 日本語, 京都女子大学, 国内会議トマトにおけるαｰトマチン生合成に関わる A環酸化還元酵素の解析
口頭発表（一般）
- 日本農芸化学会2017年度大会, 2017年03月, 日本語, 京都女子大学, 国内会議アブシジン酸に着目した根寄生雑草ストライガの寄生戦略の解析
口頭発表（一般）
- International Congress「STRIGOLACTONES」, 2017年03月, 英語, Torino , Italy, 国際会議Reductive metabolism of strigolactones in selected plants
ポスター発表
- ITbM-IGER Seminar, 2017年03月, 英語, Nagoya University, 国内会議Physiology and Biochemistry of Host-parasite Interactions in Root Parasitic Weeds,
口頭発表（一般）
- International Congress「STRIGOLACTONES」, 2017年03月, 英語, Torino , Italy, 国際会議Functional characterization of CYP711A family in sorghum and cowpea
ポスター発表
- International Congress「STRIGOLACTONES」, 2017年03月, 英語, Torino , Italy, 国際会議Diffenential pathways for conversion of carlactone to strigolactones
ポスター発表
- 日本農芸化学会2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都女子大学, 国内会議CRISPR/Cas9によるジャガイモα-ソラニン生合成遺伝子のゲノム編集
ポスター発表
- SATREPS-2 Symposium on Striga Management, 2017年01月, 英語, Khartoum, Sudan,, 国際会議Towards Maximum Utilization of the Noxious Root Parasitic Weeds, Striga spp.
口頭発表（一般）
- SATREPS-2 Symposium on Striga Management, 2017年01月, 英語, Khartoum, Sudan, 国際会議Practicality of the Suicidal Germination Approach for Controlling Striga hermonthica.
口頭発表（一般）
- SATREPS-2 Symposium on Striga Management, 2017年01月, 英語, Khartoum, Sudan, 国際会議Inhibition of Germination of Root Parasitic Weeds by Nojirimycin, an Inhibitor of Planteose Metabolism
口頭発表（一般）
- SATREPS-2 Symposium on Striga Management, 2017年01月, 英語, Khartoum, Sudan, 国際会議About the project: Development of counter measures against Striga to conquer poverty and improve food security in Sudan.
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2016年12月, 日本語, 国内会議シロイヌナズナの酸化ストレス情報伝達系に関わるシグナル受容体の探索
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2016年12月, 日本語, 国内会議ジャガイモCYP88B1のゲノム編集による有毒αーソラニンから有用サポニンへの代謝変換
口頭発表（一般）
- 平成28年度 鳥取大学乾燥地研究センター共同研究発表会, 2016年12月, 日本語, 鳥取大学乾燥地研究センター, 国内会議アブシジン酸応答に着目した根寄生雑草ストライガの生存戦略の解析
ポスター発表
- 第51回植物化学調節学会, 2016年10月, 日本語, 高知大学農林海洋科学部（物部キャンパス）, 国内会議ソルガムにおけるCYP711A酵素の機能解析
ポスター発表
- 第51回植物化学調節学会, 2016年10月, 日本語, 高知大学農林海洋科学部（物部キャンパス）, 国内会議ササゲによる合成ストリゴラクトンGR24のD環の還元
ポスター発表
- 第51回植物化学調節学会, 2016年10月, 日本語, 高知大学農林海洋科学部（物部キャンパス）, 国内会議ササゲにおけるCYP711A酵素の機能解析
ポスター発表
- 第51回植物化学調節学会, 2016年10月, 日本語, 高知大学農林海洋科学部（物部キャンパス）, 国内会議カーラクトンからストリゴラクトンへの 変換における多様性の解析
ポスター発表
- 第51回植物化学調節学会, 2016年10月, 日本語, 高知大学農林海洋科学部（物部キャンパス）, 国内会議2-Hexenalによるheat shock factorA2の発現誘導機構の解析
ポスター発表
- 第34回日本植物細胞分子生物学会, 2016年09月, 日本語, 信州大学 上田, 国内会議野生種トマトにおけるα-トマチン代謝酵素遺伝子の機能解析
ポスター発表
- The 80th Annual Meeting of the Botanical Society of Japan, 2016年09月, 英語, 沖縄コンベンションセンター, 国際会議沖縄特産トゲドコロのステロイドサポニン生合成の解析
ポスター発表
- 第34回日本植物細胞分子生物学会, 2016年09月, 日本語, 信州大学 上田, 国内会議ヤマノイモ属トゲドコロのステロイドサポニン生合成に関わる糖転移酵素の探索
ポスター発表
- The 80th Annual Meeting of the Botanical Society of Japan, 2016年09月, 英語, 沖縄コンベンションセンター, 国際会議ナス科作物のステロイドグリコアルカロイド生合成遺伝子を標的としたゲノム編集植物の解析
ポスター発表
- The 80th Annual Meeting of the Botanical Society of Japan, 2016年09月, 英語, 沖縄コンベンションセンター, 国際会議トマトにおけるαｰトマチン生合成に関わるA環酸化還元酵素の機能解析
ポスター発表
- 第34回日本植物細胞分子生物学会, 2016年09月, 日本語, 信州大学 上田, 国内会議ステロイドグリコアルカロイド生合成遺伝子CYP88B1をターゲットとした ゲノム編集ジャガイモの解析
ポスター発表
- 日本ゲノム編集学会第１回大会, 2016年09月, 英語, 広島国際会議場, 国内会議ステロイドグリコアルカロイド生合成遺伝子CYP88B1をターゲットとしたゲノム編集ジャガイモの解析
ポスター発表
- 第34回日本植物細胞分子生物学会, 2016年09月, 日本語, 信州大学 上田, 国内会議ジャガイモのα-ソラニン生合成遺伝子と共発現する機能未知遺伝子の解析
ポスター発表
- 第34回日本植物細胞分子生物学会, 2016年09月, 日本語, 信州大学 上田, 国内会議サポニン生合成遺伝子過剰発現によるトマトのトマチン生産能の増強
ポスター発表
- 20th Australasian Weeds Conference,, 2016年09月, 英語, Perth, Australia,, 国際会議Towards Maximum Utilization of the Noxious Root Parasitic Weeds. Striga spp.
ポスター発表
- 20th Australasian Weeds Conference, 2016年09月, 英語, Perth, Australia,, 国際会議Practicality of the Suicidal Germination Approach for Controlling Striga hermonthica.
ポスター発表
- 第9回六甲有機合成研究会, 2016年08月, 日本語, 国内会議根寄生植物の宿主認識に関わるストライゴラクトンの化学
口頭発表（一般）
- 植物感染生理談話会, 2016年08月, 日本語, 国内会議根寄生雑草の生存戦略とそれを支える化学
口頭発表（一般）
- Cytochrome P450 Biodiversity ＆ Biotechnology 2016, 2016年07月, 英語, Vancouver,Canada, 国際会議CRISPR/Cas9-mediated genome editing of CYP88B1: steroid glycoalkaloid biosynthetic gene in potato
ポスター発表
- 22th International Conference on Plant Growth Substances, 2016年06月, 英語, University of Toronto,Canada, 国際会議Purification and characterization of (3Z):(2E)-hexenal isomerase and its application to enhance the production of (2E)-hexena
ポスター発表
- 22th International Conference on Plant Growth Substances, 2016年06月, 英語, University of Toronto,Canada, 国際会議Identification of a sorgomol synthase, that converts 5-deoxystrigol to sorgomol in sorghum
ポスター発表
- 日本作物学会, 2016年03月, 日本語, 国内会議発芽刺激物質を用いた自殺発芽誘導による根寄生雑草ストライガの抑制
口頭発表（一般）
- 植物生理学会, 2016年03月, 日本語, 国内会議青葉アルデヒドの生成に重要な役割を果たすヘキセナールイソメラーゼの同定
口頭発表（一般）
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議青葉アルデヒドの生成に関わる酵素遺伝子の同定とトマト香気成分の改変
ポスター発表
- 平成27年度 植調関東支部雑草防除研究会・関東雑草研究会合同研究会, 2016年03月, 日本語, 文部科学省研究交流センター(茨城県つくば市）, 国内会議根寄生雑草の生存戦略の化学的解明と防除への応用
[招待有り]
口頭発表（招待・特別）
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議ヤマノイモ属のステロイドサポニン生合成に関わるCYP90B遺伝子の機能解析
ポスター発表
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議ピペコリン酸生合成酵素の解明
口頭発表（一般）
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議ナス科植物の有毒ステロイドグリコアルカロイド生合成に関わるアミノトランスフェラーゼの機能解析
ポスター発表
- 植物生理学会, 2016年03月, 日本語, 国内会議ナス科植物ステロイドグリコアルカロイドのF環形成に関わる26位アミノ基転移酵素の機能解析
口頭発表（一般）
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議トマトにおけるα-トマチン生合成に関わるステロイド3位酸化異性化酵素の解析
ポスター発表
- 熱帯農業学会, 2016年03月, 日本語, 国内会議ソルガム栽培における自殺発芽誘導を利用した根寄生雑草ストライガ防除
口頭発表（一般）
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議ソルガムにおけるsorgomol synthase遺伝子の同定及び機能解析
ポスター発表
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議ソルガムにおけるGR24水酸化反応の立体特異性と位置選択性
ポスター発表
- 日本農芸化学会大会, 2016年03月, 日本語, 国内会議ストリゴラクトン生合成経路の多様性の解明
ポスター発表
- 日本作物学会第241回講演会, 2016年, 日本語, 茨城大学, 国内会議発芽刺激物質を用いた自殺発芽誘導による根寄生雑草ストライガの抑制
口頭発表（一般）
- 日本熱帯農学会第119回講演会, 2016年, 日本語, 明治大学, 国内会議ソルガム栽培における自殺発芽誘導を利用した根寄生雑草ストライガ防除
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2015年12月, 日本語, 国内会議植物における環状アミノ酸ピペコリン酸生合成の解析
口頭発表（一般）
- Pacifichem2015, 2015年12月, 英語, Honolulu, 国際会議Strigolactones as germination stimulants for root parasitic weeds
[招待有り]
口頭発表（招待・特別）
- Pacifichem 2015, 2015年12月, 英語, Honolulu, 国際会議Manipulation of (2E)-hexenal production in plants to elucidate its roles under biotic and abiotic stress conditions
口頭発表（一般）
- Pacifichem2015, 2015年12月, 英語, 国際会議Discovery of novel targets for selective chemical control of root parasitic weeds based on metabolomic and transcriptomic analysis of their germination
口頭発表（一般）
- 日本学術会議公開シンポジウム「植物保護における外来種問題を考える」, 2015年11月, 日本語, 東京大学, 国内会議根寄生雑草の潜在的危険性と生活環に着目した防除の試み
[招待有り]
口頭発表（招待・特別）
- JSBBA KANSAI 2nd Student Forum, 2015年11月, 英語, 京都大学, 国内会議Manipulation of (2E)-hexenal production in plants to elucidate its roles under biotic and abiotic stress conditions
口頭発表（一般）
- 第50回植物化学調節学会, 2015年10月, 日本語, 東京大学, 国内会議様々な植物におけるストリゴラクトン生合成の多様性の解析
ポスター発表
- 第50回植物化学調節学会, 2015年10月, 日本語, 国内会議植物における5-deoxystrigol 立体異性体の水酸化反応とストリゴラクトン前駆体の探索
口頭発表（一般）
- 第50回植物化学調節学会, 2015年10月, 日本語, 東京大学, 国内会議根寄生雑草ヤセウツボの発芽過程における RNA-Seq によるトランスクリプトーム解析
ポスター発表
- 第50回植物化学調節学会, 2015年10月, 日本語, 東京大学, 国内会議根寄生雑草ストライガの宿主からの同化産物の収奪にはアブシジン酸に対する気孔応答の異常が関与している、
ポスター発表
- 第50回植物化学調節学会, 2015年10月, 日本語, 国内会議トマトにおけるα-トマチン生合成に関わるステロイド3位酸化異性化酵素の解明
ポスター発表
- 第50回植物化学調節学会, 2015年10月, 日本語, 国内会議ソルガムにおけるSorgomol合成酵素遺伝子の同定及び機能解析
ポスター発表
- 第50回植物化学調節学会, 2015年10月, 日本語, 東京大学, 国内会議ササゲにおけるorobanchol生合成遺伝子の解明に向けたRNA-seq解析
ポスター発表
- 第50回植物化学調節学会, 2015年10月, 日本語, 東京大学, 国内会議アブシジン酸に対するストライガの発芽と気孔の応答
ポスター発表
- 2015 SOL, 2015年10月, 英語, Bordeaux, 国際会議Characterization of aminotransferase involved in steroidal glycoalkaloids biosynthesis in Solanum plants
口頭発表（一般）
- 日本作物学会, 2015年09月, 日本語, 国内会議根寄生雑草ストライガの宿主同化産物の収奪には機構のアブシジン酸応答の異常が関与している
ポスター発表
- 日本農芸化学会中部・関西支部合同大会, 2015年09月, 日本語, 国内会議2-ヘキセナールの生成に関わる酵素遺伝子の同定とトマトの香気成分の改変
口頭発表（一般）
- 植物細胞分子生物学会, 2015年08月, 日本語, 国内会議ナス科植物ステロイドグリコアルカロイド生合成に関わるアミノ基転移酵素の機能解析
口頭発表（一般）
- XVIII International Plant Protection Congress, 2015年08月, 英語, Berlin, 国際会議Practicality of suicidal germination for combating the invasive root parasitic weed Striga hermonthica,
口頭発表（一般）
- バイオ産業研究会講演会, 2015年05月, 日本語, 国内会議根寄生雑草の発芽特性とそれを利用した防除の試み
[招待有り]
口頭発表（招待・特別）
- 日本植物生理学会, 2015年03月, 日本語, 東京農業大学, 国内会議明所高温条件で誘導されるステート２によるPSII障害回避機構の解析
口頭発表（一般）
- 植物生理学会, 2015年03月, 日本語, 国内会議傷害応答に関わる(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析
口頭発表（一般）
- 日本農芸化学会大会, 2015年03月, 日本語, 国内会議傷害によって誘導される(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析
口頭発表（一般）
- 日本農薬学会大会, 2015年03月, 日本語, 国内会議自殺発芽誘導による根寄生雑草ストライガ防除の有効性の検証
口頭発表（一般）
- 植物生理学会, 2015年03月, 日本語, 国内会議酸化ストレスシグナル分子として機能する反応性短鎖緑葉揮発性化合物
口頭発表（一般）
- 日本農薬学会大会, 2015年03月, 日本語, 国内会議根寄生雑草選択的発芽阻害剤ノジリマイシンがヤセウツボ発芽初期過程の遺伝子発現に及ぼす影響
口頭発表（一般）
- 植物生理学会, 2015年03月, 日本語, 国内会議ナス科植物ステロイドサポニンのF環の構造多様性に関わるコレステロール26位水酸化酵素の機能解析
口頭発表（一般）
- 日本農芸化学会大会, 2015年03月, 日本語, 国内会議ナス科植物ステロイドグリコアルカロイドの構造多様性に関わる生合成酵素の機能解析
口頭発表（一般）
- 日本農芸化学会大会, 2015年03月, 日本語, 国内会議ソルガムにおけるGR24水酸化反応の立体特異性と位置選択性
口頭発表（一般）
- The 1st International Congress on Strigolactones, Wageningen, 2015年03月, 英語, 国際会議Synthesis of 7-oxo-5-deoxystrigol, a 7-oxygenated strigolactone analog.
口頭発表（一般）
- The 1st International Congress on Strigolactones, Wageningen, 2015年03月, 英語, 国際会議Regioselective and stereospecific hydroxylation of GR24bby sorghum.
口頭発表（一般）
- The 1st International Congress on Strigolactones, Wageningen, 2015年03月, 英語, 国際会議Practicality of suicidal germination for combating the root parasitic weed Striga hermonthica on sorghum
口頭発表（一般）
- The 1st International Congress on Strigolactones, Wageningen, 2015年03月, 英語, 国際会議Oxidative metabolism of 5-deoxystrigol in plants.
口頭発表（一般）
- The 1st International Congress on Strigolactones, Wageningen, 2015年03月, 英語, 国際会議Heliolactone, a non-sesquiterpene lactone germination stimulant for root parasitic weeds from sunflower.
口頭発表（一般）
- 日本農薬学会第40回大会, 2015年, 日本語, 玉川大学, 国内会議自殺発芽誘導による根寄生雑草ストライガ防除の有効性の検証
口頭発表（一般）
- 1st International Congress of Strigolactones, 2015年, 英語, Wageningen, The Netherland, 国際会議Practicality of suicidal germination for combating the root parasitic weed Striga hermonthica on sorghum
ポスター発表
- 日本農芸化学会 関西支部 第487回講演会, 2014年12月, 日本語, 神戸大学大学院農学研究科 C101大教室, 国内会議傷害によって誘導される(3Z):(2E) ヘキセナールイソメラーゼの同定と酵素学的性質の解明
口頭発表（一般）
- 日本光合成学会若手の会第11回セミナー研究発表, 2014年12月, 日本語, 立命館大学びわこ草津キャンパス, 国内会議高温ストレスを受けた植物が明条件でPSII障害を回避するメカニズムの解析
口頭発表（一般）
- 第27回植物脂質シンポジウム, 2014年11月, 日本語, 静岡市産学交流センター, 国内会議(3Z):(2E) ヘキセナールイソメラーゼの同定と機能解析
口頭発表（一般）
- 第49回植物化学調節学会, 2014年10月, 日本語, 国内会議防御シグナル因子ピペコリン酸の生合成に関わるALD1の機能解析
口頭発表（一般）
- 第49回植物化学調節学会, 2014年10月, 日本語, 国内会議自殺発芽誘導によるStriga hermonthica抑制の実証
口頭発表（一般）
- 日本熱帯農業学会第116回講演会シンポジウム”熱帯農業研究と社会実装～研究成果は現地社会にいかに適応されたのか～”, 2014年10月, 日本語, 国内会議根寄生雑草ストライガの防除方法に関する研究成果とスーダン国ガダーレフ州農家への 普及活動
口頭発表（一般）
- 第49回植物化学調節学会, 2014年10月, 日本語, 国内会議ヒマワリの生産する非セスキテルペンラクトン型根寄生雑草発芽刺激物質heliolactoneの同定
口頭発表（一般）
- 第49回植物化学調節学会, 2014年10月, 日本語, 国内会議ソルガムにおけるGR24水酸化反応の立体特異性と位置選択性
口頭発表（一般）
- 日本農芸化学会関西支部会, 2014年09月, 日本語, 奈良先端科学技術大学, 国内会議光・温度条件の変化により形成されるPSI-LHCII超複合体の解析
口頭発表（一般）
- 日本農芸化学会関西支部大会, 2014年09月, 日本語, 国内会議ナス科植物ステロイドサポニンの構造多様性に関わるステロイド26位水酸化酵素の機能解析
口頭発表（一般）
- P-450, 2014年09月, 英語, 国際会議Steroid C-26 hydroxylase is involved in the structural diversity of steroidal saponins in Solanaceae.
口頭発表（一般）
- P-450, 2014年09月, 英語, 国際会議Identification of P450s involved in steroidal saponin biosynthesis from Dioscorea esculenta (Togedokoro)
口頭発表（一般）
- P-450, 2014年09月, 英語, 国際会議Characterization of two isoforms of NADPH-P450 reductase from lesser yam (Dioscorea esculenta)
口頭発表（一般）
- 第32回植物細胞分子生物学会, 2014年08月, 日本語, 国内会議根寄生挫創発芽阻害剤ノジリマイシンが及ぼすプランテオース代謝経路への影響
口頭発表（一般）
- 第32回植物細胞分子生物学会, 2014年08月, 日本語, 国内会議ナス科植物ステロイドグリコアルカロイド生合成に関わるジオキシゲナーゼの機能解析
口頭発表（一般）
- 第32回植物細胞分子生物学会, 2014年08月, 日本語, 国内会議トマト果実におけるα―トマチン代謝酵素の探索
口頭発表（一般）
- 13th IUPAC International Congress of Pesticide Chemistry, San Francisco, 2014年08月, 英語, 国際会議Study on the effect of nojirimycin on sugar metabolism in germinating seeds of root parasitic weeds for selective control.
口頭発表（一般）
- 2014 PGRSA Annual Conference, San Francisco, USA, 2014年07月, 英語, 国際会議Heliolactone, a non-sesquiterpene lactone germination stimulant for root parasitic weeds from sunflower,
口頭発表（一般）
- Third International Symposium on Broomrape in Sunflower, Cordoba, Spain, 2014年06月, 英語, 国際会議SU-01, a novel germination stimulant for root parasitic weeds from sunflower
口頭発表（一般）
- Banff Conference on Plant Metabolism Banff, Alberta, Canada,, 2014年06月, 英語, 国際会議Characterization of 2-oxoglutarate-dependent dioxygenase catalyzing C-16 hydroxylation involved in steroidal glycoalkaloids biosynthesis in Solanum plants
口頭発表（一般）
- 第5回日本光合成学会年会, 2014年05月, 日本語, 近畿大学, 国内会議コムギの光化学系における高温耐性機構の解析：ステート遷移の誘導と超高速励起型PSI-LHCII複合体の形成
ポスター発表
- 日本農薬学会, 2014年03月, 日本語, 京都大学, 国内会議根寄生雑草ヤセウツボの発芽時に発現するβ-マンノシダーゼの役割
口頭発表（一般）
- 第55回日本植物生理学会年会, 2014年03月, 日本語, 富山大学, 国内会議高温ストレスを受けたコムギにおけるチラコイド膜の構造変化を伴う光化学系の再編成：熱放散とステート遷移の誘導
ポスター発表
- 日本植物生理学会, 2014年03月, 日本語, 国内会議ヤマノイモ属トゲドコロのステロイドサポニン生合成に関わるP450の同定
口頭発表（一般）
- 李ヒョン宰, 2014年03月, 日本語, 国内会議ヤマノイモ属トゲドコロに蓄積するステロイドサポニンの単離精製および定量解析
口頭発表（一般）
- 日本農芸化学会大会, 2014年03月, 日本語, 国内会議ヤセウツボ発芽種子で発現するβ-マンノシダーゼの機能解析
口頭発表（一般）
- 日本農芸化学会大会2014年度東京大会, 2014年03月, 日本語, 国内会議ソルガムにおける5-deoxystrigolからsorgomolへの変換反応
[招待有り]
口頭発表（招待・特別）
- 日本作物学会, 2014年03月, 日本語, 国内会議ストライガの土壌中種子密度および生態型が陸稲品種の抵抗性に及ぼす影響
口頭発表（一般）
- 日本農芸化学会大会, 2014年03月, 日本語, 国内会議ジャガイモの有毒ソラニン生合成に関わる新規ジオキシゲナーゼの機能解析
口頭発表（一般）
- The 3rd Pest Management Conference of the Sudan, Wad Medani, Sudan, 2014年02月, 英語, Sudan, 国際会議Evaluation of resistance of upland rice varieties to Striga hermonthica through laboratory, pot and field experiments.
口頭発表（一般）
- 植物化学調節学会第49回大会, 2014年, 日本語, 京都大学, 国内会議自殺発芽誘導によるStriga hermonthica抑制の実証
ポスター発表
- 日本作物学会第237回講演会, 2014年, 日本語, 第237回, 国内会議ストライガの土壌中種子密度および生態型が陸稲品種の抵抗性に及ぼす影響
口頭発表（一般）
- SATREPS International Symposium on Striga Management, 2014年, 英語, Khartoum, Sudan, 国際会議Identification of Striga-resistant upland rice varieties adapted to growth conditions in Sudan and durability of their resistance
口頭発表（一般）
- The 3rd Pest Management Conference of the Sudan, 2014年, 英語, Wad Medani, Sudan, 国際会議Evaluation of resistance of upland rice varieties to Striga hermonthica through laboratory, pot and field experiments
口頭発表（一般）
- SATREPS International Symposium on Striga Management, 2014年, 英語, Khartoum, Sudan, 国際会議Activity and stability of T-010 in soil and demonstration of suicidal germination for combating Striga hermonthica
口頭発表（一般）
- 第48回大会植物化学調節学会, 2013年10月, 日本語, 国際会議植物における5-デオキシストリゴールからモノヒドロキシストリゴラクトンへの変換
口頭発表（一般）
- 第47回植物化学調節学会, 2013年10月, 日本語, 国内会議根寄生植物Strigagesnerioides種子のストライゴラクトンに対する構造要求性の解明
口頭発表（一般）
- 植物化学調節学会第48回大会, 2013年10月, 日本語, 国内会議根寄生雑草の選択的防除法確立に資するヤセウツボ発芽時の糖代謝に関する研究
ポスター発表
- 第48回大会植物化学調節学会, 2013年10月, 日本語, 国内会議β－カロテン生産菌を用いたストライゴラクトン生合成中間体カルラクトン酵素合成の試み
口頭発表（一般）
- 植物化学調節学会第48回大会, 2013年10月, 日本語, 国内会議ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成16位水酸化酵素の同定
口頭発表（一般）
- 植物化学調節学会第48回大会, 2013年10月, 日本語, 国内会議ヒマワリが生産する根寄生雑草発芽刺激物質の解析
口頭発表（一般）
- 植物化学調節学会第48回大会, 2013年10月, 日本語, 国内会議ナス科植物ステロイドグリコアルカロイド生合成に関わる新規2-オキソグルタル酸依存性ジオキシゲナーゼ：16位水酸化酵素の同定と機能解析
口頭発表（一般）
- 植物化学調節学会第48回大会, 2013年10月, 日本語, 国内会議S. gesnerioides D14-like惟謙氏の探索とストライゴラクトン加水分解活性の評価
口頭発表（一般）
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議ヤマノイモ属トゲドコロ由来のフロスタノール配糖体を加水分解するβ-グルコシダーゼの同定
口頭発表（一般）
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議ヤマノイモ属トゲドコロ由来NADPH-P450還元酵素遺伝子の単離同定
口頭発表（一般）
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成P-450遺伝子の探索
口頭発表（一般）
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議ノジリマイシンの糖代謝阻害による根寄生雑草選択的な発芽抑制
ポスター発表
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議トマト果実におけるα-トマチン代謝酵素遺伝子の探索
ポスター発表
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議トマトにおけるステロイドアルカロイド生合成に関わる配糖化酵素の探索
ポスター発表
- 日本農芸化学会関西・中四国・西日本支部合同大会, 2013年09月, 日本語, 国内会議シロイヌナズナ由来葉緑体局在Lonプロテアーゼの異種発現と酵素学的解析
口頭発表（一般）
- 第31回植物細胞分子生物学会, 2013年09月, 日本語, 国内会議アグロインフィルトレーション法による植物発現系を用いた植物代謝酵素の機能解析
口頭発表（一般）
- 第31回植物細胞分子生物学会, 2013年09月, 英語, 国内会議Identification and quantification of steroidal saponins in Dioscorea esculenta
口頭発表（一般）
- 住友化学健康・農業関連事業研究所, 2013年07月, 日本語, 国内会議根寄生植物の生活環調節とストライゴラクトン
[招待有り]
口頭発表（招待・特別）
- World Congress of Parasitic Plants, 2013年07月, 英語, 国際会議The bioconversion of 5-deoxystrigol to monohydroxylated strigolactones by plants
口頭発表（一般）
- 12th World Congress of Parasitic Plants, 2013年07月, 英語, 国際会議Structural requirements of strigolactones for germination induction and inhibition of Striga gesnerioides seeds
口頭発表（一般）
- 12th World Congress of Parasitic Plants, 2013年07月, 英語, 国際会議Inhibitory effect of nojirimycin on germination and sugar metabolism of a broomrape
口頭発表（一般）
- 12th World Congress of Parasitic Plants, 2013年07月, 英語, 国際会議Functional characterization of a -mannosidase involved in the early germination process of Orobanche minor
口頭発表（一般）
- 12th World Congress of Parasitic Plants, 2013年07月, 英語, 国際会議Evaluation of resistance of upland rice varieties to Striga hermonthica through laboratory, pot and field experiments
口頭発表（一般）
- TERPNET2013, 2013年06月, 英語, 国際会議Characterization of furostanol glycoside 26-O-β-glucosidase involved in hydrolysis of protodioscin from Dioscorea esculenta
口頭発表（一般）
- TERPNET2013, 2013年06月, 英語, 国際会議Characterization of De90B, cholesterol 22-hydroxylase involved in steroidal saponin biosynthesis in the tubers of Dioscorea esculenta
口頭発表（一般）
- 日本農芸化学会大会（2013 Mar）, 2013年03月, 日本語, 国内会議根寄生植物Striga種子のストライゴラクトンに対する構造要求性の解明
口頭発表（一般）
- 日本農芸化学会大会, 2013年03月, 日本語, 国内会議根寄生雑草ヤセウツボの発芽過程におけるノジリマイシンによる糖代謝阻害と発芽抑制の関連性
口頭発表（一般）
- 日本植物生理学会, 2013年03月, 日本語, 国内会議高温ストレスを受けた植物において再編成される光化学系分子メカニズムの解析
口頭発表（一般）
- 日本作物学会, 2013年03月, 日本語, 国内会議ライゾトロン法でストライガ抵抗性を示した陸稲品種の野外試験での評価
口頭発表（一般）
- 日本農芸化学会大会, 2013年03月, 日本語, 国内会議ヤマノイモ属における有用ステロイドサポニン生合成に関わる β-グルコシダーゼの同定
口頭発表（一般）
- 日本農芸化学会大会, 2013年03月, 日本語, 国内会議ヤセウツボ発芽種子で発現するβ-マンノシダーゼの機能解析
口頭発表（一般）
- 日本農薬学会第38回大会, 2013年03月, 日本語, 国内会議ノジリマイシンによる根寄生雑草ヤセウツボ種子の発芽と糖代謝への影響
口頭発表（一般）
- 第54回日本植物生理学会年会, 2013年03月, 日本語, 国内会議Functional characterization of a β-mannosidase involved in the early germination process of the root-parasitic weed Orobanche minor
口頭発表（一般）
- 日本作物学会第235回講演会, 2013年, 日本語, 明治大学, 国内会議ライゾトロン法でストライガ抵抗性を示した陸稲品種の野外試験での評価
口頭発表（一般）
- The 6th Asia and Oceania Conference on Photobiology, 2013年, 英語, 国際会議Remodeling of photosystems to induce thermal dissipation and state transition accompanied by structural changes of thylakoid membranes in heat-stressed wheat
ポスター発表
- 12th World Congress on Parasitic Plants, 2013年, 英語, Sheffield, UK, 国際会議Evaluation of resistance of upland rice varieties to Striga hermonthica through laboratory, pot and field experiments
ポスター発表
- 植物化学調節学会, 2012年10月, 日本語, 国内会議様々な植物における5-deoxystrigol の酸化的代謝
口頭発表（一般）
- 第47回植物化学調節学会, 2012年10月, 日本語, 国内会議ヒマワリが生産するストライゴラクトンの探索
口頭発表（一般）
- 第47回植物化学調節学会, 2012年10月, 日本語, 国内会議ナス科植物におけるステロイドグリコアルカロイド生合成に関わるP450の酵素解析
口頭発表（一般）
- 植物化学調節学会, 2012年10月, 日本語, 国内会議ソルガムによる5-deoxystrigolからsorgomolへの変換
口頭発表（一般）
- 植物化学調節学会, 2012年10月, 日本語, 国内会議Strigolactoneの生理活性における最小構造
口頭発表（一般）
- Nijmegen University, 2012年10月, 英語, 国際会議Strigolactones, New Plant Hormones, Importance of their stereochemistry for bioactivity as germination stimulant
[招待有り]
口頭発表（招待・特別）
- 植物化学調節学会, 2012年10月, 日本語, 国内会議Striga gesnerioides種子に対する発芽阻害物質の探索
口頭発表（一般）
- Wageningen University, 2012年10月, 英語, 国際会議Stereochemistry and Biosynthesis of Strigolactones, New Plant Hormones
[招待有り]
口頭発表（招待・特別）
- 10th International Congress on Plant Molecular Biology, 2012年10月, 英語, 国際会議Oxidative Bioconversion of a Synthetic Strigolactone Analog GR24 in the Root Cultures of Menispermum dauricum,
口頭発表（一般）
- International Congress on Plant Molecular Biology, 2012年10月, 英語, 国際会議Oxidation of 5-deoxystrigol in sorghum, Sorghum bicolor (L.) Moech
口頭発表（一般）
- 10th International Congress on Plant Molecular Biology, 2012年10月, 英語, 国際会議D1 protein is degraded in heat-stressed plants in the dark, presumably due to electron inflow of the stromal reducing power into the plastoquinone.
口頭発表（一般）
- Palacky University, 2012年10月, 英語, 国際会議Bioactivity, Stereochemistry and Biosynthesis of Strigolactones, New Plant Hormones
[招待有り]
口頭発表（招待・特別）
- International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議Translocation of host materials to parasite: Stomatal response and photosynthetic capacity of Striga hermonthica and sorghum under water stress,
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議The project on improvement of food security in semi-arid regions of Sudan through management of root parasitic weeds: An introductory note on project activities
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議T-010 a novel compound for induction of suicidal germination of Striga hermonthica,
口頭発表（一般）
- 植物微生物研究会, 2012年09月, 日本語, 国内会議Structure-activity relationship of strigolactone analogs as a phytohormone in rice and germination stimulants toward root parasitic weeds、
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議Orobanche minor germination and interaction with red clover
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議Isolation of ACC synthase gene from Striga gesnerioides,
口頭発表（一般）
- , International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議Evaluation of resistance of upland rice varieties to Striga hermonthica through laboratory, pot and field experiments
口頭発表（一般）
- Cytochromes P450 in Steroid Glycoalkaloids Biosynthesis in Solanum Plants、植物二次代謝のフロンティアシンポジウム- 今後の二次代謝を語る！, 2012年09月, 日本語, 国内会議Cytochromes P450 in Steroid Glycoalkaloids Biosynthesis in Solanum Plants
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年09月, 英語, 国際会議Bioassay-guided purification of germination stimulants produced by sasame
口頭発表（一般）
- 日本植物細胞分子学会, 2012年08月, 日本語, 国内会議ヤマノイモ属における有用ステロイドサポニン生合成に関わるβ-グルコシダーゼの解析
口頭発表（一般）
- 平成25年度日本植物細胞分子学会, 2012年08月, 日本語, 国内会議コウモリカズラにおける含塩素イソキノリンアルカロイド 生合成中間体の同定
口頭発表（一般）
- AgroBioInstutute, Sofia, Bulgaria, 2012年07月, 英語, 国際会議Root Parasitic Weed Research in Kobe University
[招待有り]
口頭発表（招待・特別）
- 11th International Symposium on Cytochrome P450, 2012年06月, 英語, 国際会議Isolation and characterization of furostanol 26-O-glucoside -glucosidase involved in hydrolysis of protodioscin, furostanol saponin from Dioscorea esculenta, based on transcriptome analysis of the rhizomes
口頭発表（一般）
- International Society of Root Research, Dundee, UK, 2012年06月, 英語, 国際会議Gas exchange and stomatal response of root parasitic weed Striga hermonthica and sorghum under water stress
口頭発表（一般）
- 日本作物学会, 2012年03月, 日本語, 国内会議陸稲の異なる三種のストライガ抵抗性についてライゾトロン法を用いた評価
口頭発表（一般）
- 日本農芸化学会大会, 2012年03月, 日本語, 国内会議根寄生雑草Striga gesnerioides種子発芽誘導物質alectrolの単離と構造決定
口頭発表（一般）
- 日本農芸化学会大会, 2012年03月, 日本語, 国内会議合成ストライゴラクトンGR24 の植物による代謝と生成物の同定
口頭発表（一般）
- 日本熱帯農学会, 2012年03月, 日本語, 国内会議高温低湿度条件において潅水頻度と播種時期がNERICA4の収量に及ぼす影響
口頭発表（一般）
- 日本植物生理学会, 2012年03月, 日本語, 国内会議高温ストレスを受けた植物がステート遷移によりPSIIを保護するメカニズムの解析
口頭発表（招待・特別）
- 日本農芸化学会大会, 2012年03月, 英語, 国内会議ヤマノイモ属トゲドコロにおけるプロトジオシンを加水分解するβ－グルコシダーゼの解析
口頭発表（一般）
- 日本農芸化学会2012年大会, 2012年03月, 日本語, 国内会議LC-MS/MSによる新奇ストリゴラクトン探索法の確立
口頭発表（一般）
- 日本作物学会第233回講演会, 2012年, 日本語, 東京農工大学, 国内会議陸稲の異なる三種のストライガ抵抗性についてライゾトロン法を用いた評価
口頭発表（一般）
- 日本熱帯農学会第111回講演会, 2012年, 日本語, 東京農工大学, 国内会議高温低湿度条件において潅水頻度と播種時期が NERICA4 の収量に及ぼす影響
口頭発表（一般）
- 第53回日本植物生理学会年会, 2012年, 日本語, 国内会議高温ストレスを受けた植物がステート遷移によりPSIIを保護するメカニズムの解析
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年, 英語, Khartoum, Sudan, 国際会議Translocation of host materials to parasite: Stomatal response and photosynthetic capacity of Striga hermonthica and sorghum under water stress
口頭発表（一般）
- 6th International Crop Science Congress, 2012年, 英語, Bento Gonçalves, Brazil, 国際会議Growth and yield of NERICA4 under field conditions in Sudan
口頭発表（一般）
- International Seminar on SATREPS Project for Striga Management, 2012年, 英語, Khartoum, Sudan, 国際会議Evaluation of resistance of upland rice varieties to Striga hermonthica through laboratory, pot and field experiments
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2011年12月, 日本語, 国内会議高温ストレスを受けたコムギにおいてステート遷移により移動するLHCⅡタンパク質の解析
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2011年12月, 日本語, 国内会議Solanacolの光学活性体合成に関する研究
口頭発表（一般）
- SOL & ICuGI 2011, 2011年12月, 英語, 国際会議Exploratory studies of the metabolic genes involved in biosynthesis and metabolism of steroid glycoalkaloid tomatine in tomato
口頭発表（一般）
- Japan-Korea Plant Biotechnology for the Next Generation, Narita, 2011年12月, 英語, 国内会議A novel strategy of parasitic weed control focusing on specific metabolism in germinating seeds
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議土壌乾燥条件下での根寄生雑草ストライガとソルガムのガス交換と気孔反応
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議水酸化GR24 の合成と構造解析
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議植物によるGR24 変換反応の解析
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 英語, 国内会議根寄生植物ヤセウツボの発芽を阻害するノジリマイシンの作用点
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議根寄生雑草Striga gesnerioides 種子の発芽を誘導するストライゴラクトンの構造要求性
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成に関与する遺伝子の探索
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議ソルガムにおける5-deoxystrigol の酸化的代謝
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議ストライゴラクトンの枝分かれ抑制活性における構造要求性
口頭発表（一般）
- 植物化学調節学会第46回大会, 2011年11月, 日本語, 国内会議. 根寄生雑草Striga gesnerioides 種子発芽を誘導するササゲ由来ストライゴラクトンの単離同定
口頭発表（一般）
- 日本農芸化学会西支部中部支部合同大会, 2011年10月, 日本語, 国内会議植物が光化学系の高温障害を回避するメカニズムの解析
口頭発表（一般）
- 日本生物工学会, 2011年09月, 日本語, 国内会議放線菌Streptomyces ficellusによる寄生植物発芽種子阻害剤ノジリマイシンの生産
口頭発表（一般）
- 植物細胞分子生物学会, 2011年09月, 日本語, 国内会議根寄生植物ヤセウツボの発芽種子におけるゲンチアノース代謝経路の解明
口頭発表（一般）
- 天然有機化合物討論会, 2011年09月, 日本語, 国内会議根寄生雑草Striga gesnerioides種子発芽刺激物質の立体化学
口頭発表（一般）
- 日本生物工学会, 2011年09月, 日本語, 国内会議寄生植物ヤセウツボの発芽種子における希少三糖ゲンチアノースの代謝経路の解明
口頭発表（一般）
- 植物細胞分子生物学会, 2011年09月, 日本語, 国内会議ヤマノイモ属トゲドコロにおけるステロイドサポニン生合成酵素の探索
口頭発表（一般）
- 植物細胞分子生物学会, 2011年09月, 日本語, 国内会議トマト果実における-トマチンの代謝酵素の探索
口頭発表（一般）
- 植物細胞分子生物学会, 2011年09月, 日本語, 国内会議トマトにおけるα-トマチン生合成酵素の探索
口頭発表（一般）
- 植物細胞分子生物学会, 2011年09月, 日本語, 国内会議クマリン類生合成の鍵酵素である桂皮酸オルト位水酸化酵素の解析
口頭発表（一般）
- 東大農学部 フードサイエンス棟 中島董一郎記念ホール（2011 July 1）, 2011年07月, 日本語, 東大農学部 フードサイエンス棟 中島董一郎記念ホール（2011 July 1）, 国内会議根寄生植物と宿主植物の相互作用
[招待有り]
口頭発表（招待・特別）
- 11th World Congress on Parasitic Plants, 2011年06月, 英語, 国際会議Sugar metabolism during germination of Orobanche minor as a novel target for selective control
口頭発表（一般）
- 11th World Congress on Parasitic Plants (Martina Franca, 2011年06月, 英語, 国際会議Structural requirements of strigolactones for induction of germination in root parasitic plants
口頭発表（一般）
- 11th World Congress on Parasitic Plants, 2011年06月, 英語, 国際会議Promotive and inhibitory stereoisomers of strigolactones to seed germination of Striga gesnerioides
口頭発表（一般）
- ・11th World Congress on Parasitic Plants, 2011年06月, 英語, 国際会議Germination stimulants for Striga gesnerioides from cowpea (Vigna unguiculata)
口頭発表（一般）
- TERPENET, 2011年05月, 英語, 国際会議Transcriptome analysis of the rhizomes of Dioscorea spp. to investigate steroidal saponin biosynthesis
口頭発表（一般）
- 日本農芸化学会大会, 2011年03月, 日本語, 国内会議寄生雑草ヤセウツボの発芽に関与するゲンチアノース代謝関連酵素の解析
口頭発表（一般）
- 日本作物学会第231回講演会, 2011年03月, 日本語, 国内会議異なる土壌水分条件下での根寄生雑草ストライガとソルガムの気孔反応と光合成活性
口頭発表（一般）
- 日本作物学会第231回講演会, 2011年03月, 日本語, 国内会議ライゾトロン法およびスーダン現地でのポット試験による陸稲品種のストライガ感受性の評価
口頭発表（一般）
- 日本農芸化学会関西・中部支部合同大会, 2011年, 日本語, 国内会議植物が光化学系の高温障害を回避するメカニズムの解析
口頭発表（一般）
- 第53回天然有機化合物討論会, 2011年, 日本語, 大阪, 国内会議根寄生雑草Striga gesnerioides種子発芽刺激物質の立体化学
ポスター発表
- 日本農芸化学会関西支部例会, 2010年12月, 日本語, 国内会議植物における高反応性カルボニル化合物のNADPH依存解毒機構
口頭発表（一般）
- RIHN Satellite Symposium for IAS International Conference, 2010年12月, 英語, 国内会議Allelopathic Activity of Mesquite (Prosopis juliflora) and Screening of Allelopathic Plants in Sudan
口頭発表（一般）
- 植物化学調節学会第45回大会, 2010年11月, 日本語, 国内会議根寄生植物オロバンキの成長過程における脱分化・分化の制御機構の解明
口頭発表（一般）
- 植物化学調節学会第45回大会, 2010年11月, 日本語, 国内会議根寄生雑草ヤセウツボの発芽に関わる糖代謝酵素の解析
口頭発表（一般）
- 植物化学調節学会第45回大会, 2010年11月, 日本語, 国内会議メスキート葉に由来する根寄生植物の種子発芽抑制物質の探索
口頭発表（一般）
- 植物化学調節学会第45回大会, 2010年11月, 日本語, 国内会議キュウリ緑葉からの-不飽和カルボニル化合物還元酵素の精製と遺伝子解析
口頭発表（一般）
- 植物化学調節学会第45回大会, 2010年11月, 日本語, 国内会議Gentiana urnula Harry Sm. の花由来の新規抗酸化成分
口頭発表（一般）
- 植物化学調節学会第45回大会, 2010年11月, 日本語, 国内会議.植物におけるクマリン類生合成の鍵酵素である桂皮酸オルト位水酸化酵素の解析
口頭発表（一般）
- 10th International Symposium in Woods Hole, 2010年10月, 英語, 国際会議Exploring P450s involved in ABA catabolism from Bryophyte and Pteridophyte, P450 Biodiversity and Biotechnology
口頭発表（一般）
- SATREPS-JSPS AA Science Platform Program Joint Seminar on Striga spp., the food security, 2010年09月, 英語, 国内会議Stomatal response and photosynthetic capacity of Striga and sorghum under water stress
口頭発表（一般）
- SATREPS-JSPS AA Science Platform Program Joint Seminar on Striga spp., the food security, 2010年09月, 英語, 国内会議Qualitative and quantitative analysis of strigolactones using LC-MS/MS
口頭発表（一般）
- SATREPS-JSPS AA Science Platform Program Joint Seminar on Striga spp., the food security, 2010年09月, 英語, 国内会議Control of parasitic weeds by disturbing their crucial metabolic pathways
口頭発表（一般）
- 日本農芸化学会, 2010年03月, 日本語, 国内会議根寄生植物ヤセウツボの成長過程の形態学的観察および植物ホルモン分析
口頭発表（一般）
- 日本農薬学会, 2010年03月, 日本語, 国内会議寄生雑草ヤセウツボの発芽に関与する糖分解酵素に関する研究
口頭発表（一般）
- 日本農芸化学会, 2010年03月, 日本語, 国内会議リンドウ科Gentiana urnula Harry Sm. の花由来の新規抗酸化成分について
口頭発表（一般）
- 日本植物生理学会, 2010年03月, 日本語, 国内会議コケおよびシダ植物におけるABA不活性化P450の探索
口頭発表（一般）
- 日本農芸化学会, 2010年03月, 日本語, 国内会議キュウリ緑葉由来NADPH 依存性Alkenal/one oxidoreductase の精製と遺伝子解析
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2010年02月, 日本語, 国内会議マロンジアルデヒドによるタンパク質修飾が光化学系IIの機能に与える影響
口頭発表（一般）
- JSPS AA platform program and the Research Institute of Humanity and Nature (RIHN) Seminar on the Noxious Weeds Striga hermonthica and Prosopis juliflora, 2009年12月, 英語, 国際会議Sugar and nitrogen metabolism in germinating seeds of parasitic weeds
口頭発表（一般）
- 農芸化学会, 2009年11月, 日本語, 国内会議ベンジルイソキノリンアルカロイド生合成に関わるシトクロムP-450遺伝子の単離
口頭発表（一般）
- 第24回農薬デザイン研究会, 2009年11月, 日本語, 国内会議ストリゴラクトンの合成と生物活性
口頭発表（一般）
- JSPS AA platform program and the Research Institute of Humanity and Nature (RIHN) Seminar on the Noxious Weeds Striga hermonthica and Prosopis juliflora, 2009年11月, 英語, 国際会議Germination response of Striga hermonthica and Orobanche minor seeds pre-treated with the synthetic strigolactone GR24
口頭発表（一般）
- JSPS AA platform program and the Research Institute of Humanity and Nature (RIHN) Seminar on the Noxious Weeds Striga hermonthica and Prosopis juliflora, 2009年11月, 英語, 国際会議About the Seminar - Towards a sustainable management of the noxious weeds mesquite (Prosopis spp.)And Striga hermonthica in Sub Saharan Africa
口頭発表（一般）
- 植物化学調節学会, 2009年10月, 日本語, 国内会議根粒共生がオロバンキの寄生に及ぼす影響
口頭発表（一般）
- 植物化学調節学会, 2009年10月, 日本語, 国内会議根寄生植物ヤセウツボの寄生成立後の生長過程に関する形態学的観察
口頭発表（一般）
- 植物化学調節学会2009, 2009年10月, 日本語, 国内会議根寄生植物の種子発芽刺激活性に対するストライゴラクトンB環の修飾の影響について
口頭発表（一般）
- 植物化学調節学会, 2009年10月, 日本語, 国内会議柑橘およびマメ科植物におけるクマリン生合成酵素の探索
口頭発表（一般）
- 植物化学調節学会, 2009年10月, 日本語, 国内会議メスキートが生産する有用二次代謝産物の検索
口頭発表（一般）
- 植物化学調節学会, 2009年10月, 日本語, 国内会議ブラシノステロイド生合成に関与すると推定されるコケおよびシダ植物P-450の機能解
口頭発表（一般）
- 植物化学調節学会, 2009年10月, 日本語, 国内会議イソキノリンアルカロイド生合成に関わるシトクロムP-450 cDNAの単離および機能解析
口頭発表（一般）
- 6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009年09月, 英語, 国際会議Water transport in xylem elements in the parasitic interaction of host (Lotus japonicus) and
口頭発表（一般）
- 25th Naito Conference, 2009年09月, 英語, 国際会議Unravelling coumarin biosynthesis in plants and in vivo synthesis in E. coli
口頭発表（一般）
- Plant Biology 2009, 2009年07月, 英語, 国際会議Unique primary metabolism during seed germination of root parasitic plants
口頭発表（一般）
- 10th World Congress on Parasitic Plants, 2009年07月, 英語, 国際会議Metabolome analysis of Orobanche minor seed germination for selective control of parasitic
口頭発表（一般）
- 10th World Congress on Parasitic Plants, 2009年06月, 英語, 国際会議Induction of phytoalexin biosynthesis in Lotus japonicus roots in response to Striga hermonthica attachment
口頭発表（一般）
- 農芸化学会, 2009年03月, 日本語, 国内会議飽和型GR24のヤセウツボ（Orobanche minor）に対する種子発芽抑制と寄生抑制効果の検討
口頭発表（一般）
- 農芸化学会, 2009年03月, 日本語, 国際会議寄生植物の発芽過程における代謝変動解析
口頭発表（一般）
- 農芸化学会, 2009年03月, 日本語, 国内会議ベンジルイソキノリンアルカロイド生合成に関わるシトクロムP-450遺伝子の単離2009.Mar
口頭発表（一般）
- 植物生理学会, 2009年03月, 日本語, 国内会議. 暗所での高温ストレスによるPSII活性の減少にはNADP(H) dehydrogenaseが関与する
口頭発表（一般）
- JSPS International Seminar “Devastating Effects of Striga on African Agriculture and Development of Countermeasures”, 2008年12月, 英語, 国際会議Stomatal response of Striga and host plant under water stress and foliar application of ABA
口頭発表（一般）
- JSPS International Seminar “Devastating Effects of Striga on African Agriculture and Development of Countermeasures” (2008 Dec), 2008年12月, 英語, 国際会議Background of the project “Ecophysiological Aspects of the Root Parasitic Weeds Striga spp. and Development of Control Measures”
口頭発表（一般）
- 竹松セミナー, 2008年11月, 日本語, 国内会議宿主植物による根寄生植物の認識
口頭発表（招待・特別）
- 植物化学調節学会, 2008年10月, 日本語, 国内会議根寄生植物に対するミヤコグサの応答の分子解析
口頭発表（一般）
- 植物化学調節学会, 2008年10月, 日本語, 国内会議根寄生植物と宿主植物の相互作用に関する生物有機化学的研究
口頭発表（招待・特別）
- 植物化学調節学会, 2008年10月, 日本語, 国内会議根寄生植物Striga hermonthicaおよびOrobanche minorの種子発芽過程におけるエチレン
口頭発表（一般）
- 植物化学調節学会, 2008年10月, 日本語, 国内会議ストライガの接触によるミヤコグサでのファイトアレキシン生合成の誘導
口頭発表（一般）
- 植物微生物研究会, 2008年09月, 日本語, 国内会議非親和性根寄生植物ストライガはミヤコグサのファイトアレキシン生合成を活性化する
口頭発表（一般）
- 植物微生物研究会, 2008年09月, 日本語, 国際会議根寄生植物に対するミヤコグサの分子応答
口頭発表（一般）
- 植物微生物研究会, 2008年09月, 日本語, 国内会議根寄生植物に対するミヤコグサの応答
口頭発表（一般）
- 植物細胞分子生物学会, 2008年09月, 日本語, 国内会議根寄生植物O. minorの種子発芽に対するエチレンの関与の検証
口頭発表（一般）
- 植物細胞分子生物学会, 2008年09月, 日本語, 国内会議デクロロダウリカミン脱水素酵素の精製と酵素学的性質
口頭発表（一般）
- 4th Pan Pacific Conference on Pesticide Science, 2008年06月, 英語, 国際会議Synthesis and seed stimulating activity of some oxime derivatives.
口頭発表（一般）
- 日本農芸化学会薮田セミナー バイオマスデザインとリファイナリー －競合から共存へ－, 2008年05月, 日本語, 国内会議根寄生雑草の防除による植物バイオマス増産
口頭発表（招待・特別）
- 農業環境技術研究所 生物多様性研究領域セミナー, 2008年05月, 英語, 国内会議Physiology and Ecology of Root Parasitic Plants
口頭発表（招待・特別）
- 日本農芸化学大会, 2008年03月, 日本語, 国内会議根寄生植物の寄生に対するミヤコグサの応答の分子解析
口頭発表（一般）
- 日本植物生理学会シンポジウム, 2008年03月, 日本語, 国内会議高温ストレス下のアラビドプシスにおけるアモンジアルデヒドの二面的な生理機能
口頭発表（招待・特別）
- 日本植物生理学会, 2008年03月, 日本語, 国内会議寄生植物Striga hermonthicaの光受容体フィトクロム相同遺伝子の単離および光応答の解析
口頭発表（一般）
- 日本農芸化学大会, 2008年03月, 日本語, 国内会議FAD依存性Dechlorodauricumine酸化酵素の精製と酵素学的性質
口頭発表（一般）
- 日本農薬学会, 2008年03月, 日本語, 国内会議.根寄生植物の発芽刺激物質ストライゴラクトンの合成と生物活性
口頭発表（一般）
- 科学技術振興調整費重要課題解決型プロジェクト「外来植物のリスク評価と蔓延防止策」, 2008年02月, 日本語, 国内会議寄生植物の生態と防除～その脅威と防除法について～
口頭発表（招待・特別）
- 日本農芸化学会関西支部例会, 2007年12月, 日本語, 国内会議高温処理を施したシロイヌナズナにおいて蓄積するアルデヒドによる修飾を受けたタンパク質の探索と解析
口頭発表（一般）
- 日本農芸化学会関西支部例会, 2007年12月, 日本語, 国内会議ストライゴラクトンの合成と発芽刺激活性
口頭発表（一般）
- JSPS/JST Intl Symposium on Toward Advanced Use of African Resources in Plant Science, 2007年11月, 英語, 国際会議Phytochromes in parasitic plants, Orobanche minor and Striga hermonthica
口頭発表（一般）
- JSPS/JST Intl Symposium on Toward Advanced Use of African Resources in Plant Science, 2007年11月, 英語, 国際会議Molecular responses of sorghum roots to Striga hermonthica parasitism
口頭発表（一般）
- JSPS/JST Intl Symposium on Toward Advanced Use of African Resources in Plant Science, 2007年11月, 英語, 国内会議Collaborative activities between Kobe University and ARC, Sudan
口頭発表（一般）
- 植物化学調節学会, 2007年10月, 日本語, 国内会議根寄生植物－宿主植物間相互作用の分子解析（Ⅲ）ストライガの寄生に対して感受性が異なるソルガムの分子応答
口頭発表（一般）
- 植物化学調節学会, 2007年10月, 日本語, 国内会議根寄生植物の寄生に対するミヤコグサの応答の分子解析
口頭発表（一般）
- 兵庫バイオテクノロジー研究会, 2007年10月, 日本語, 国内会議寄生植物の生存戦略と宿主植物の応答
口頭発表（招待・特別）
- 植物細胞分子生物学会, 2007年08月, 日本語, 国内会議前培養中の刺激物質処理が根寄生植物種子のエチレン生合成および発芽に与える影響の解析
口頭発表（一般）
- 植物細胞分子生物学会, 2007年08月, 日本語, 国内会議寄生植物Striga hermonthica由来光受容体フィトクロム相同遺伝子の単離
口頭発表（一般）
- 植物細胞分子生物学会, 2007年08月, 日本語, 国内会議コウモリカズラ培養根より調製した粗酵素による含塩素アルカロイドの生合成前駆体の変換
口頭発表（一般）
- 9th World Congress on Parasitic Plants, 2007年07月, 英語, 国際会議Molecular analysis of Lotus japonicus response against Orobanche aegyptiaca and Striga hermonthica parasitism
口頭発表（一般）
- 9th World Congress on Parasitic Plants, 2007年06月, 英語, 国際会議In vitro production of strigolactones by plant root cultures
口頭発表（招待・特別）
- ショ糖を基質とした三酵素反応によるガラクチノールの合成, 2007年05月, 日本語, 国内会議ショ糖を基質とした三酵素反応によるガラクチノールの合成
口頭発表（一般）
- 日本農芸化学大会, 2007年03月, 日本語, 国内会議親和性の根寄生植物Orobanche crenataおよび非親和性のStriga hermonthicaの侵入に対するミヤコグサの応答の分子解析
口頭発表（一般）
- 日本農芸化学大会, 2007年03月, 日本語, 国内会議光照射下で高温ストレスを受けたシロイヌナズナにおいてマロンジアルデヒドはリノレン酸から生じてタンパク質を化学修飾する
口頭発表（一般）
- 日本農芸化学大会, 2007年03月, 日本語, 国内会議ミヤコグサ根による(+)-5-deoxystrigol培養生産
口頭発表（一般）
- The Bi-national Japan-Israel Workshop on Aspects of parasitic weeds: biology and control, 2007年03月, 英語, 国際会議Molecular analysis of Lotus japonicus response against Orobanche crenata and Striga hermonthica parasitism
口頭発表（招待・特別）
- 日本農芸化学大会, 2007年03月, 日本語, 国内会議Dechlorodauricumineはコウモリカズラ培養根における含塩素アルカロイドの生合成前駆体である
口頭発表（一般）
- 日本農芸化学大会, 2007年03月, 日本語, 国内会議Alectrolの化学構造について
口頭発表（一般）
- 植物化学シンポジウム, 2006年12月, 日本語, 国内会議根圏情報物質ストライゴラクトンの化学と機能
口頭発表（招待・特別）
- 日本農芸化学会関西支部例会, 2006年12月, 日本語, 国内会議高温ストレスを受けたシロイヌナズナにおけるアルデヒド生成機構の解析と被修飾タンパク質の探索
口頭発表（一般）
- SPS AA Science Platform Program, 2006年11月, 英語, 国際会議Marker assisted breeding of stay-green trait to enhance drought tolerance in sorghum.
口頭発表（一般）
- JSPS AA Science Platform Program, 2006年11月, 英語, 国際会議Interactions between the parasitic weed Striga hermonthica and its host Sorghum bicolor at a
口頭発表（一般）
- IUPAC International Conference of Pesticide Chemistry, 2006年08月, 英語, 国際会議Strigolactones, germination stimulants for parasitic weeds Striga and Orobanche; action mechanisms, in vitro prodeuction and biosynthesis
口頭発表（招待・特別）
- 植物化学調節学会, 2005年, 日本語, 植物化学調節学会, 東京大学, 国内会議根寄生植物－宿主植物間相互作用の分子解析（Ⅰ）ストライガの寄生に対するソルガムの応答
口頭発表（一般）
- 日本植物生理学会年会, 2005年, 日本語, 日本植物生理学会, 新潟, 国内会議高温と活性酸素が引き起こすリノレン酸の過酸化が葉緑体タンパク質の化学修飾をもたらす
口頭発表（一般）
- 日本農芸化学会2005年度大会講演要旨集p.5, 2005年, 日本語, 日本農芸化学会, 札幌, 国内会議コウモリカズラにおける含塩素アルカロイド生合成前駆体の単離
口頭発表（一般）
- 植物化学調節学会, 2005年, 日本語, 植物化学調節学会, 東京大学, 国内会議カプセル化による根寄生雑種種子発芽刺激物質の安定化
口頭発表（一般）
- JSPS AA Science Plantform Program Seminar, 2005年, 英語, 未記入, 未記入, 国際会議Our approaches to control the root parasitic weeds Striga and Orobanche
口頭発表（一般）
- Pacifichem2005, 2005年, 英語, 未記入, 未記入, 国際会議Molecular interactions between the parasitic weed Striga hermonthica and its host Sorghum bicolor
口頭発表（一般）
- JSPS AA Science Plantform Program Seminar, 2005年, 英語, 未記入, 未記入, 国際会議Germination strategy of Striga hermonthica involves regulation of ethylene biosynthesis
口頭発表（一般）
- 69th Annual Meeting of Plant Society of Japan, Symposium, 2005年, 英語, 未記入, 未記入, 国内会議Germination stimulants for seeds of root parasitic weeds
口頭発表（一般）
- 日本農薬学会大会, 2004年, 日本語, 日本農薬学会, 神戸, 国内会議様々な条件で調製したスクロース結晶の融点の多様性
口頭発表（一般）
- 植物化学調節学会, 2004年, 日本語, 植物化学調節学会, 秋田県立大学, 国内会議根寄生植物研究におけるモデル宿主植物としてのミヤコグサ
口頭発表（一般）
- 日本農芸化学会関西支部例会第437回講演会, 2004年, 日本語, 日本農芸化学会関西支部, 神戸大学, 国内会議ミヤコグサ培養根が生産する根寄生植物種子発芽刺激物質の精製
口頭発表（一般）
- 日本農薬学会大会, 2004年, 日本語, 日本農薬学会, 神戸, 国内会議ミヤコグサが生産する根寄生雑草ストライガ種子発芽刺激物質の精製
口頭発表（一般）
- 日本農芸化学会関西支部第437回講演会, 2004年, 日本語, 日本農芸化学会関西支部, 神戸大学, 国内会議コウモリカズラ培養根からのdechlorodauricumineの単離・構造決定
口頭発表（一般）
- 植物化学調節学会, 2003年10月, 日本語, 植物化学調節学会, 名古屋大学, 国内会議ワタの生産する根寄生雑草発芽刺激物質
口頭発表（一般）
- 日本農芸化学会大会, 2003年03月, 日本語, 日本農芸化学会, 名城大学, 国内会議宿主・非宿主植物による根寄生雑草発芽刺激物質の生産
口頭発表（一般）
- 日本農薬学会大会, 2003年03月, 日本語, 日本農薬学会, 名城大学, 国内会議根寄生植物種子発芽刺激物質の植物界における分布－Part 1
口頭発表（一般）
- 日本農薬学会大会, 2003年03月, 日本語, 日本農薬学会, 名城大学, 国内会議根寄生雑草Striga hermonthica の発芽におけるエチレンの役割
口頭発表（一般）
- 6th Intl. Symposium on Structure and Function of Root, 2003年, 英語, 未記入, 未記入, 国際会議Siliconinduced changes in visicoelastic properties of root cell walls in Sorghum
口頭発表（一般）
- 6th Intl. Symposium on Structure and Function of Root, 2003年, 英語, 未記入, 未記入, 国際会議Root development, nodule root emergence and exudation rate in two sorghum cultivars differed from drought tolerance
口頭発表（一般）
- PGRSA/JSCRP, 2003年, 英語, PGRSA, JSCRP, 未記入, 国際会議Distribution of strigol analogues, germination stimulants for Striga and Orobanche among host and nonhost plants
口頭発表（一般）
- PGRSA/JSCRP, 2003年, 英語, Plant Growth Regulation Society of America, 植物化学調節学会, 国際会議A kinase highly homologous to PKA from parasitic plant
口頭発表（一般）
所属学協会
American Society of Plant Physiologists
International Parasitic Plant Society
日本農薬学会
日本植物細胞分子生物学会
植物化学調節学会
日本農芸化学会
共同研究・競争的資金等の研究課題
- 基盤研究(C), 科学研究費補助金, 2022年04月 - 2025年03月ストリゴラクトンのBC環形成に関わる立体制御機構の解明
- 国立研究開発法人科学技術振興機構, 国際科学技術共同研究推進事業 地球規模課題対応国際科学技術協力プログラム, 2016年 - 2022年03月, 研究代表者根寄生雑草の発芽機構および宿主抵抗性に着目した防除法の開発ならびに有用性の探索
競争的資金
- 科学研究費補助金／基盤研究(B), 2015年04月 - 2019年03月, 研究代表者
競争的資金
- 科学研究費一部基金／基盤研究(B), 2013年04月 - 2017年03月, 研究代表者
競争的資金
- 独立行政法人日本学術振興会, 二国間国際交流（スーダン(OP)との共同研究）, 2017年, 研究代表者スーダンにおける根寄生雑草ストライガの防除と利用を目指した天然物化学研究の展開
競争的資金
- 独立行政法人日本学術振興会, 二国間交流事業（オープンパートナーシップ共同研究）, 2016年, 研究代表者スーダンにおける根寄生雑草ストライガの防除と利用を目指した天然物化学研究の展開
競争的資金
- 日本学術振興会, 二国間交流事業（オープンパートナーシップ共同研究）, 2015年, 研究代表者二国間交流「スーダンにおける根寄生雑草ストライガの防除と利用を目指した天然物化学研究の展開」
競争的資金
- 科学研究費補助金／萌芽研究, 2012年04月 - 2014年03月, 研究代表者
競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2014年, 研究代表者SATREPS「根寄生雑草克服によるスーダン乾燥地農業開発」
競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2013年, 研究代表者SATREPS「根寄生雑草克服によるスーダン乾燥地農業開発」
競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2012年, 研究代表者SATREPS「根寄生雑草克服によるスーダン乾燥地農業開発」
競争的資金
- 科学研究費補助金／基盤研究(B), 2011年, 研究代表者
競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2011年, 研究代表者SATREPS「根寄生雑草克服によるスーダン乾燥地農業開発」
競争的資金
- 2010年, 研究代表者寄生雑草ストライガの生理生態学的特性の解析と防除戦略の構築
競争的資金
- 2009年, 研究代表者寄生雑草ストライガの生理生態学的特性の解析と防除戦略の構築
競争的資金
- 科学研究費補助金／基盤研究(B), 2008年, 研究代表者
競争的資金
- 2008年, 研究代表者寄生雑草ストライガの生理生態学的特性の解析と防除戦略の構築
競争的資金
- 2007年, 研究代表者寄生雑草ストライガの生理生態学的特性の解析と防除戦略の構築
競争的資金
- 2007年, 研究代表者スーダンにおける食糧生産の増大と安定化を目指した水資源管理と寄生雑草の防除
競争的資金
- 科学研究費補助金／基盤研究(B), 2005年, 研究代表者
競争的資金
- 科学研究費補助金／基盤研究(C), 2005年, 研究代表者
競争的資金
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