研究者紹介システム

勝田 知尚
カツダ トモヒサ
大学院工学研究科 応用化学専攻
准教授
応用化学関係
Last Updated :2022/06/23

研究者情報

所属

  • 【主配置】

    大学院工学研究科 応用化学専攻
  • 【配置】

    工学部 応用化学科, 環境保全推進センター

学位

  • 博士(工学), 大阪市立大学
  • 化学工学に基づく生物プロセスの効率化と分析

授業科目

ジャンル

  • 科学・技術 / バイオテクノロジー

コメントテーマ

  • フォトバイオリアクター
  • バイオエネルギー
  • バイオレメディエーションによる鉛回収
  • 光合成微生物による有用物質生産

研究活動

研究キーワード

  • 生物化学工学
  • 微細藻類
  • クロマトグラフィー

研究分野

  • ものづくり技術(機械・電気電子・化学工学) / バイオ機能応用、バイオプロセス工学

委員歴

  • 2017年04月 - 2019年03月, 化学工学会, 関西支部委員
  • 2012年03月 - 2018年02月, 化学工学会, バイオ部会庶務幹事
  • 2003年04月 - 2012年12月, 日本生物工学会, 関西支部委員
  • 2002年04月 - 2011年10月, Young Asian Biochemical Engineers’ Community, 日本事務局員
  • 2002年10月 - 2010年12月, 日本生物工学会関西支部, バイオテクノロジー実験講座講師
  • 2000年04月 - 2009年03月, 化学工学会関西支部CES21, 運営委員

受賞

  • 2018年11月 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of influenza virus-like particles in stably transformed insect cells

    MATSUDA Takuya, TANIJIMA Toshikazu, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa, YAMAJI Hideki

    国際学会・会議・シンポジウム等の賞

  • 2017年09月 化学工学会, バイオ部会優秀ポスター賞, 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産

    溝手 結, 増見 恭子, 勝田 知尚, 山地 秀樹

    国内学会・会議・シンポジウム等の賞

  • 2016年11月 Japanese Association for Animal Cell Technology (JAACT), Best Poster Presentation Award, Production of virus-like particles using recombinant insect cells

    TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki

    国際学会・会議・シンポジウム等の賞

  • 2010年10月 Asia Pacific Confederation of Chemical Engineering, Best Poster Paper Award (The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010)), Production of Japanese encephalitis virus-like particles in insect cell expression systems

    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

    国際学会・会議・シンポジウム等の賞

  • 2010年02月 化学工学会関西支部, 化学工学会バイオ部会, 第8回最先端バイオテクノロジー発表会ポスター賞, 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響, 野上達弘

    野上 達弘, 木原 麻那, 佐野 宰久, 勝田 知尚, 山地 秀樹

  • 2009年12月 Young Asian Biochemical Engineers’ Community, Best Poster Presentation Award for an Original Contribution to the 15th Symposium of Young Asian Biochemical Engineers’ Community, Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli

    KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

  • 2007年09月 日本生物工学会, 生物工学奨励賞(照井賞), フォトバイオリアクターによる有用物質生産のための生物化学工学的検討

    勝田 知尚

  • 2005年03月 化学工学会, 平成16年度化学工学会奨励賞(内藤雅喜記念賞), 光合成微生物による光培養生産プロセスの開発

    勝田 知尚

  • 2018年10月 神戸大学工学部, 平成29年度優秀教育賞(応用化学科)

    勝田 知尚

  • 2007年09月 神戸大学工学部, 平成18年度優秀教育賞(移動現象論・分離工学分野)

    大村 直人, 今駒 博信, 鈴木 洋, 勝田 知尚

論文

  • OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki

    Monoclonal antibodies and antibody fragments are widely used in therapeutics and diagnoses. While mammalian cells serve as the host cells for antibody production, insect cells can produce large quantities of secretory antibodies in serum-free suspension cultures. The effects of lithium on the processes of autophagy and apoptosis in mammalian cells are well chronicled. In the present study, stably transformed insect cells, which produce an engineered antibody molecule, were cultured with lithium chloride in a serum-free medium. Treatment with lithium chloride induced autophagy and apoptosis in recombinant insect cells and led to increases in the yields of secreted antibodies.

    SPRINGER, 2019年01月, In Vitro Cellular & Developmental Biology - Animal, 55 (1), 1 - 6, 英語

    [査読有り]

    研究論文(学術雑誌)

  • LEONG Hui Yi, OOI Chien Wei, LAW Chung Lim, JULKIFLE Advina Lizah, KATSUDA Tomohisa, SHOW Pau Loke

    Recently, betacyanins have added research applications in food science processing industries, owing to their health promoting functional and attractive visual properties. Additionally, red-purple pitaya (Hylocereus polyrhizus) is a rich source of betacyanins. Development of a green and effective bioseparation technology is deemed favourably in the downstream bioprocessing industries. Hence, this study utilised a liquid biphasic electric flotation (LBEF) system for betacyanins extraction from peel and flesh of red-purple pitaya as well as comparison between LBF (liquid biphasic flotation) and LBEF system, color characterisation and antioxidant activity evaluation were conducted. In this study, the optimised LBF system was integrated with electric system. Collectively, the betacyanins extraction using LBEF system showed an optimal betacyanins extraction from the peel and flesh, with the significant highest values of betacyanins concentration in alcohol-rich top phase (C-t) (99.014 +/- 0.074% and 96.132 +/- 0.154%), separation efficiency (E) (98.383 +/- 0.215% and 96.576 +/- 0.083%) and partition coefficient (K) (100.814 +/- 7.324 and 24.883 +/- 1.052) of betacyanins. Moreover, the peel and flesh extract showed different variations of red colors and antioxidant properties. The present study introduces a new, simple and high efficient bioseparation technology (LBEF system) which is definitely worth to study further.

    ELSEVIER SCIENCE BV, 2019年, Separation and Purification Technology, 209, 193 - 201, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa

    2018年10月, New Biotechnology, 44S, S159, 英語

    研究論文(国際会議プロシーディングス)

  • Revathy Sankaran, Pau Loke Show, Yee Jiun Yap, Yang Tao, Tau Chuan Ling, Katsuda Tomohisa

    Liquid biphasic flotation (LBF) system has been recognized as an efficient, green, economically sustainable and biocompatible technique for biomolecules separation and purification. The main drawbacks of the conventional process of biomolecules separation are expensive production cost, utilization of phase components that are inefficiently recycled and global pollution due to high chemical consumption and wastage. In this paper, a novel approach of LBF system for lipase recovery utilizing recycling phase components comprising surfactant and xylitol was investigated. The scope of this study focuses on pollution prevention as well as clean and environmentally friendly process for enzyme extraction via LBF. The green process proposed in this study uses phase-forming components that have recovery and recycling abilities for minimal use of chemicals for enzyme extraction. This novel method utilized Triton X-100 and xylitol for lipase extraction from Burkholderia cepacia. A few parameters were optimized to obtain high lipase separation efficiency and yield. Based on the ideal conditions of LBF, the average lipase separation efficiency and yield are 86.46 and 87.49%, correspondingly. Phase components recycling were proposed in order to reduce the chemicals consumption in LBF system. Upscaling of the recycling study exhibited consistent result with the laboratory scale. It was found that 97.20 and 98.67% of Triton X-100 and xylitol were recovered after five times of recycling and that a total of 75.87% of lipase separation efficiency was obtained. Recovery and recycling of phase components in the extraction process are established as the principal green chemistry method, which yields high separation efficiency and is economically feasible on an industrial scale.

    Springer Verlag, 2018年04月05日, Clean Technologies and Environmental Policy, 20 (9), 1 - 12, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa

    2018年03月, BMC Proceedings, 12 (3), 19, 英語

    研究論文(国際会議プロシーディングス)

  • LEE Xin Jiat, SHOW Pau Loke, KATSUDA Tomohisa, CHEN Wei-Hsin, CHANG Jo-Shu

    Membrane bioreactor (MBR) is regarded as the state-of-the-art technology in separation processes. Surface modification techniques play a critical role in improving the conventional membrane system which is mostly hydrophobic in nature. The hydrophobic nature of membranes is known to cause fouling, resulting in high maintenance costs and shorter lifespan of MBR. Thus, surface grafting aims to improve the hydrophilicity of bio-based membrane systems. This review describes the major surface grafting techniques currently used in membranes, including photo induced grafting, plasma treatment and plasma induced grafting, radiation induced grafting, thermal induced grafting and ozone induced grafting. The advantages and disadvantages of each method is discussed along with their parametric studies. The potential applications of MBR are very promising, but some integral membrane properties could be a major challenge that hinders its wider reach. The fouling issue could be resolved with the surface grafting techniques to achieve better performance of MBRs.

    ELSEVIER SCI LTD, 2018年, Bioresource Technology, 269, 489 - 502, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Keita Mori, Hirotsugu Hamada, Takafumi Ogawa, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji

    Transient gene expression allows a rapid production of diverse recombinant proteins in early-stage preclinical and clinical developments of biologics. Insect cells have proven to be an excellent platform for the production of functional recombinant proteins. In the present study, the production of an antibody Fab fragment by transient gene expression in lepidopteran insect cells was investigated. The DNA fragments encoding heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of an Fab fragment were individually cloned into the plasmid vector plHAneo, which contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression. Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were co-transfected with the resultant plasmid vectors using linear polyethyleneimine. When the transfection efficiency was evaluated, a plasmid vector encoding an enhanced green fluorescent protein (EGFP) gene was also co-transfected. Transfection and culture conditions were optimized based on both the flow cytometry of the EGFP expression in transfected cells and the yield of the secreted Fab fragments determined by enzyme-linked immunosorbent assay (ELISA). Under optimal conditions, a yield of approximately 120 mg/L of Fab fragments was achieved in 5 days in a shake-flask culture. Transient gene expression in insect cells may offer a promising approach to the high-throughput production of recombinant proteins. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2017年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124 (2), 221 - 226, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideki Yamaji, Keita Mori, Hirotsugu Hamada, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda

    ELSEVIER SCIENCE BV, 2016年07月, NEW BIOTECHNOLOGY, 33, S200 - S201, 英語

    研究論文(国際会議プロシーディングス)

  • Shinya Takada, Takafumi Ogawa, Kazusa Matsui, Tasuku Suzuki, Tomohisa Katsuda, Hideki Yamaji

    The generation of a recombinant baculovirus that displays antibody Fab fragments on the surface was investigated. A recombinant baculovirus was engineered so that the heavy chain (Hc; Fd fragment) of a mouse Fab fragment was expressed as a fusion to the N-terminus of baculovirus gp64, while the light chain of the Fab fragment was simultaneously expressed as a secretory protein. Following infection of Sf9 insect cells with the recombinant baculovirus, the culture supernatant was analyzed by enzyme-linked immunosorbent assay using antigen-coated microplates and either an anti-mouse IgG or an anti-gp64 antibody. A relatively strong signal was obtained in each case, showing antigen-binding activity in the culture supernatant. In western blot analysis of the culture supernatant using the anti-gp64 antibody, specific protein bands were detected at an electrophoretic mobility that coincided with the molecular weight of the Hc-gp64 fusion protein as well as that of gp64. Flow cytometry using a fluorescein isothiocyanate-conjugated antibody specific to mouse IgG successfully detected the Fab fragments on the surface of the Sf9 cells. These results suggest that immunologically functional antibody Fab fragments can be displayed on the surface of baculovirus particles, and that a fluorescence-activated cell sorter with a fluorescence-labeled antigen can isolate baculoviruses displaying specific Fab fragments. This successful baculovirus display of antibody Fab fragments may offer a novel approach for the efficient selection of specific antibodies.

    SPRINGER, 2015年08月, CYTOTECHNOLOGY, 67 (4), 741 - 747, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Luca Giannelli, Hiroyuki Yamada, Tomohisa Katsuda, Hideki Yamaji

    The green alga Haematococcus pluvialis, which accumulates astaxanthin at an optimal temperature of 20 degrees C, was cultivated under temperatures of 20 degrees C, 23.5 degrees C, 27 degrees C, and 30.5 degrees C, in order to assess the effects on algal metabolism during the growth phase. The culture growth rate declined with above-optimal increases in temperature, and the final maximum cell concentration at 30.5 degrees C reached only 35% of that attained at 20 degrees C. On the contrary, the biomass productivity was increased under all the high-temperature conditions, probably reflecting the metabolism switch from cell duplication to energy accumulation that is typically observed in algal cultures subjected to environmental stress. Moreover, an increase in the light-harvesting capability of the alga was observed by means of the total pigment balance and the photosynthesis-intensity (PI) curve measured under the different cultivation conditions. Cultures kept at higher temperatures were able to better harvest and utilize the impinging light due to photo-acclimation. Finally, the differences in the astaxanthin metabolism were elucidated by subjecting the cultures to nitrogen starvation at 20 degrees C and 27 degrees C. In the culture at 27 degrees C, a 1.4-fold increase in the astaxanthin productivity was observed when compared to that at 20 degrees C, and the latter required almost two-fold more energy for the astaxanthin production compared with the 27 degrees C culture. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2015年03月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119 (3), 345 - 350, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Luca Giannelli, Hideki Yamaji, Tomohisa Katsuda

    Vertical photobioreactors (PBR) with cylindrical cross section, namely air-lift reactors (ALR) and bubble column reactors (BCR), are often chosen both for bench-scale and industrial scale microalgal cultivation. It was common belief that ALR was the most favorable configuration in terms of light conversion effciency (LCE) and/or photosynthetic productivity than BCR because of the regular cyclic-flow pattern achieved inside the PBR. In the present study, we simulated the flow patterns in both ALR and BCR by means of computational fluid dynamics (CFD) and clarified the effects of such flow pattern on the LCE and productivity. Simulation results, obtained from the open-source CFD suite OpenFOAM, showed good agreement both for the flow velocity and the mixing time observed in the actual PBR using high-speed photography and conductivity pulse response, respectively. Subsequently, Lagrangian particle tracking was conducted on the simulation results to highlight the main fluid-flow patterns and to calculate the local-flashing-light frequency, which was necessary in order to estimate the overall light conversion effciency of the PBR. The BCR was characterized by a highly random fluid pattern with macroscopic, low-frequency circular loops while the ALR was characterized by numerous swirling flows localized inside the draft tube in addition to the main recirculation between the inner and outer portions of the tube. Finally, image analysis was used to correlate the numerical calculations with the light conversion effciencies attained in a Haematococcus pluvialis culture that had been illuminated with flashing light.

    SOC CHEMICAL ENG JAPAN, 2015年01月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 48 (1), 61 - 71, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hideki Yamaji, Tomohisa Katsuda, Hirotsugu Hamada, Keita Mori

    ELSEVIER SCIENCE BV, 2014年07月, NEW BIOTECHNOLOGY, 31, S186 - S186, 英語

    研究論文(国際会議プロシーディングス)

  • Hideki Yamaji, Masataka Nakamura, Miwa Kuwahara, Yusuke Takahashi, Tomohisa Katsuda, Eiji Konishi

    The production of Japanese encephalitis (JE) virus-like particles (VLPs) in stably transformed lepidopteran insect cells was investigated. The DNA fragment encoding the JE virus (JEV) prM signal peptide, the precursor (prM) of the viral membrane protein (M), and the envelope glycoprotein (E) was cloned into the plasmid vector pIHAbla. The pIHAbla contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with a blasticidin resistance gene for use as a selectable marker. DNA encoding a form of prM with a pr/M cleavage site mutation was used to suppress the cell-fusion activity of VLPs. After transfection with the resultant plasmid, Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were incubated with blasticidin, and cells resistant to the antibiotic were obtained. Western blot analysis and enzyme-linked immunosorbent assay of a culture supernatant showed that transfected High Five cells secreted an E antigen equivalent to the authentic JEV E. Sucrose density-gradient sedimentation analysis of the culture supernatant from recombinant High Five cells indicated that secreted E antigen molecules were produced in a particulate form. VLPs recovered from the supernatant successfully induced neutralizing antibodies in mice, particularly when adsorbed to alum adjuvant. High yields (a parts per thousand 30 mu g/ml) of E antigen were achieved in shake-flask cultures. These results indicate that recombinant insect cells may offer a novel approach for efficient VLP production.

    SPRINGER, 2013年02月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97 (3), 1071 - 1079, 英語

    [査読有り]

    研究論文(学術雑誌)

  • YAMAJI Hideki, SEGAWA Maiko, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

    The production of a secreted form of Japanese encephalitis (JE) virus-like particles (VLPs) using the baculovirus-insect cell system was investigated. A recombinant baculovirus that contained the JE virus (JEV) prM signal sequence and the genes encoding the precursor (prM) of the viral membrane protein (M) and the envelope glycoprotein (E) was constructed. Western blotting and enzyme-linked immunosorbent assay (ELISA) of the culture supernatant showed that Spodoptera frugiperda Sf9 cells infected with the recombinant baculovirus had secreted the E protein. Sucrose density-gradient sedimentation analysis of the culture supernatant suggested that secreted E antigen molecules were in a particulate form. Baculovirus-infected Sf9 cells produced more than a 10-fold higher yield of E antigen than that produced by previously reported recombinant CHO cells. Following infection with a recombinant baculovirus encoding a form of prM with a pr/M cleavage site mutation designed to suppress cell-fusion activity of E, Sf9 cells showed an E antigen yield comparable to a yield obtained with the baculovirus encoding the authentic form of prM. Baculovirus-infected Trichoplusia ni BIT-TN-5B1-4 (High Five) cells secreted less of the E antigen than Sf9 cells. Moreover, the Drosophila BiP signal sequence gave an E antigen yield comparable to the prM signal sequence, while the honeybee melittin signal sequence and the baculovirus gp64 signal sequence resulted in lower yields of the E antigen. These results provide information important to the development of VLP production processes using the baculovirus insect cell system. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2012年12月, Journal of Bioscience and Bioengeering, 114 (6), 657 - 662, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Development of Liposome Preparation, Using Gas-Liquid Phase Adsorption

    Hirohiko AIHARA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Ruri HIDEMA

    2012年11月, International Workshop on Process Intensification 2012, 51 - 52, 英語

    研究論文(国際会議プロシーディングス)

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

    We describe the secretory production of a mouse anti-bovine ribonuclease A single-chain variable fragment (scFv) fused with the Fc region of human IgG1 in stably transformed lepidopteran insect cells. Use of the insect-derived Bip and melittin signal peptides resulted in higher yields of the secreted scFv-Fc fusion protein than use of the baculovirus gp64 signal peptide. After cotransfection with an expression vector that contains the Bip signal sequence upstream of the DNA encoding the scFv-Fc and a selection vector that carries a neomycin resistance gene, Trichoplusia ni BT1-TN-5B1-4 (High Five) cells were cultured in the presence of G418. Colonies of resistant cells were obtained around 2 weeks after adding G418, and clonal cells were screened by enzyme-linked immunosorbent assay (ELISA) of the culture supernatant. The yield of the scFv-Fc protein secreted from the most productive clone was around 60 mg/l in a shake-flask culture. To improve the productivity, we investigated the effect of medium supplements of sodium butyrate (NaBu), dimethyl sulfoxide (DMSO), and sericin. Supplementing culture medium with sericin increased the scFv-Fc protein yield to 82 mg/l, but productivity was not increased by either NaBu or DMSO. These results indicate that the stably transformed insect cells may allow for the efficient production of scFv-Fc and other Fc fusion proteins. (C) 2012 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2012年08月, BIOCHEMICAL ENGINEERING JOURNAL, 67 (-), 77 - 82, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroyoshi Miyahara, Ryou Nakashima, Masaki Inoue, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

    A silica-based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica-based medium were studied during repeated use and compared with other protein A media available on the market. The silica-based medium provided high dynamic binding capacities for human polyclonal IgG in the superficial liquid velocity range from 150 to 720?cm?h1 because of a high mass transfer coefficient and high mechanical strength. The dynamic binding capacity and IgG recovery were high and varied only slightly during 50150 cycles of repeated use. The release of protein A ligand was low.

    WILEY-BLACKWELL, 2012年01月, CHEMICAL ENGINEERING & TECHNOLOGY, 35 (1), 157 - 160, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomohisa Katsuda, Hiroyuki Sonoda, Yoichi Kumada, Hideki Yamaji

    Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine-Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed. © 2012 Springer Science+Business Media, LLC.

    2012年, Methods in Molecular Biology, 907, 305 - 324, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

    The effects of cytoplasmic and periplasmic chaperones on the secretory production of an anti-bovine ribonuclease A single-chain variable fragment (scFv) 3A21 in Escherichia coli were investigated. Co-expression of a cytoplasmic chaperone, GroEL/ES, DnaK/DnaJ/GrpE, trigger factor, or SecB with 3A21 scFv affected the proportions of antigen-binding activity in the cytoplasmic soluble fraction, the periplasmic fraction, and the extracellular medium, but there was no significant difference in the total activity compared to the control without chaperone co-expression. On the other hand, co-expression of a periplasmic chaperone, Skp or FkpA, with the exception of DsbC, greatly increased the binding activity in all the soluble fractions. Co-expression of both Skp and FkpA had no synergistic effect. Combinations of cytoplasmic and periplasmic chaperones decreased the productivity. In shake-flask cultures of cells co-expressing Skp or FkpA, considerable amounts of 3A21 scFv were detected in the extracellular medium by enzyme-linked immunosorbent assay (ELISA) and Western blot, and the extracellular production level of 3A21 scFv was calculated to be around 40mg/l. The binding activity of 3A21 scFv co-expressed with Skp was slightly higher than that with FkpA. These results indicate that the co-expression of periplasmic chaperones Skp and FkpA is extremely useful for the secretory production of scFvs in a culture medium using E. coli, but cytoplasmic chaperones and multiple-chaperone combinations may not be effective. Copyright (C) 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2011年04月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111 (4), 465 - 470, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

    We describe the soluble production of a mouse anti-bovine ribonuclease A single-chain variable fragment (scFv) fused with the Fc region of human IgG1 in Escherichia coli. Two production systems, secretory production using a pelB signal peptide and cytoplasmic production in a trxB/gor double mutant strain with an oxidizing cytoplasm, were investigated for efficient production of soluble and functional scFv-Fc fusion protein. Antigen-binding activity was observed in both systems but almost all of the scFv-Fc that was expressed formed insoluble aggregates. Hence, the co-expression of molecular chaperones was examined. Co-expression of GroEL/GroES showed a 4.6-fold increase in antigen-binding activity in the cytoplasmic production system but not in the secretory system. By contrast, the other two chaperones, DnaK/DnaJ/GrpE and trigger factor, had no effect in either production system. The protein solubility was also improved markedly by the co-expression of GroEL/GroES and approximately 70% of the 3A21 scFv-Fc protein was soluble. A practical productivity of more than 10 mg/L was achieved with a simple batch shake-flask culture. These results indicate that the E. coli cytoplasmic production system with oxidizing cytoplasm and molecular chaperones might be one of the choices for the soluble production of scFv-Fcs and other Fc fusion proteins. (c) 2010 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2011年02月, BIOCHEMICAL ENGINEERING JOURNAL, 53 (3), 253 - 259, 英語

    [査読有り]

    研究論文(学術雑誌)

  • FURUTA Takanori, OGAWA Takafumi, KATSUDA Tomohisa, FUJI Ikuo, YAMAJI Hideki

    The production of an Fab fragment of the catalytic antibody 6D9 in lepidopteran insect cells infected with a recombinant baculovirus that contained both the heavy chain (Hc) and light chain (Lc) genes of the Fab fragment was investigated. Western blotting and enzyme-linked immunosorbent assay (ELISA) of culture supernatant showed that baculovirus-infected Trichoplusia ni BTI-TN-5B1-4 (High Five) cells secreted an Fab fragment that retained antigen-binding activity. Infection of High Five cells with a recombinant baculovirus, in which the Lc and Hc genes were located downstream of the baculovirus p10 and polyhedrin promoters, respectively, produced a higher Fab fragment yield than that obtained with a baculovirus in which the Hc and Lc genes were downstream of the p10 and polyhedrin promoters, respectively. Baculovirus-infected High Five cells secreted more of the Fab fragment than Spodoptera frugiperda Sf9 cells. Moreover, use of the baculovirus gp64 signal sequence upstream of the Lc and Hc genes resulted in greater yield of the secreted Fab fragment than use of the insect-derived BiP and melittin signal sequences. Consequently, the Fab fragment was obtained in a high yield (>600 mu g/ml) in a shake-flask culture of High Five cells infected at a multiplicity of infection (MOI) of 10 plaque-forming units (pfu)/cell with the recombinant baculovirus in which the Lc and Hc genes with the gp64 signal sequence were expressed under the control of the p10 and polyhedrin promoters, respectively. These results indicate that the baculovirus-insect cell system may allow efficient production of antibody Fab fragments. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2010年11月, Journal of Bioscience and Bioengineering, 110 (5), 577 - 581, 英語

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    研究論文(学術雑誌)

  • Production of Japanese encephalitis virus-like particles in insect cell expression systems

    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

    2010年10月, The 13th Asia Pacific Confederation of Chemical Engineering Congress Conference Proceeding, 10640, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Naoya Morishita, Tomohisa Katsuda, Shuji Kubo, Akinobu Gotoh, Hideki Yamaji

    Adenovirus vector production by anchorage-independent 293 cells immobilized using porous biomass support particles (BSPs) was investigated in static and shake-flask cultures for efficient large-scale production of adenovirus vectors for gene therapy applications. The density of cells immobilized within BSPs was evaluated by measuring their WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) reduction activity. In shake-flask culture, 293-F cells, which were adapted to serum-free suspension culture, were not successfully retained within reticulated polyvinyl formal (PVF) resin BSPs (2 x 2 x 2 mm cubes) with matrices of relatively small pores (pore diameter 60 mu m). When the BSPs were coated with a cationic polymer polyethyleneimine, a high cell density of more than 10(7) cells cm(-3)-BSP was achieved in both static and shake-flask cultures with regular replacement of the culture medium. After infection with an adenovirus vector carrying the enhanced green fluorescent protein gene (Ad EGFP), the specific Ad EGFP productivity of the immobilized cells was comparable to the maximal productivity of non-immobilized 293-F cells by maintaining favorable conditions in the culture environment.

    SPRINGER, 2010年08月, CYTOTECHNOLOGY, 62 (4), 293 - 300, 英語

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    研究論文(学術雑誌)

  • Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji

    The production of a single-chain variable fragment (scFv) antibody against bovine ribonuclease A in the cytoplasm of Escherichia coli trxB/gor double mutant was investigated. Previous reports have shown that the thioredoxin (Trx) protein fusion strategy is useful for the correct folding of scFvs and that the expression of functional scFvs is increased by co-expression of molecular chaperones. In the present study, we examined the effects of the combination of Trx fusion and molecular chaperone co-expression on the production of a functional scFv. A Trx-fused scFv was obtained in the oxidizing cytoplasm, and co-expression of GroELS and trigger factor had the greatest effect, resulting in a 2.8-fold increase in specific productivity. By contrast, the molecular chaperone DnaKJE had no effect. Moreover, co-expression of DnaKJE with GroELS negated the effects of GroELS. Trx-scFv was purified using a bovine ribonuclease A-coupled Sepharose column, and 2.7 mg/L of purified protein was obtained. Soluble Trx-scFv, expressed and purified as described above, exhibited pH-dependent binding similar to that of the parental full-length antibody. In addition, approximately 80% of the initial binding activity was retained after incubation at 37 degrees C for 2 weeks, indicating that the Trx-scFv fusion protein is quite stable. This strategy might be useful for the preparation of other recombinant scFvs. (C) 2009 Elsevier Inc. All rights reserved.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, 2010年04月, PROTEIN EXPRESSION AND PURIFICATION, 70 (2), 248 - 253, 英語

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    研究論文(学術雑誌)

  • Hideki Yamaji, Yusuke Takahashi, Masataka Nakamura, Tomohisa Katsuda, Miwa Kuwahara, Eiji Konishi

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108 (S1), S7 - S7, 英語

    研究論文(国際会議プロシーディングス)

  • Mayu Fukui, Shinji Shiomi, Naoki Tanuma, Tomohisa Katsuda, Naofumi Shiomi, Hideki Yamaji

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108 (1), S93 - S94, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Juan Huang, Na Jin, Tomohisa Katsuda, Hideki Fukuda, Hideki Yamaji

    Escherichia coli cells were efficiently immobilized in reticulated polyvinyl formal resin biomass support particles (BSPs) that had been simply autoclaved with a solution of a cationic polymer such as polyethyleneimine. When the immobilized E. coli cells containing aspartase were used as whole cell biocatalyst for L-aspartic acid production, they showed specific aspartase activity comparable to that of non-immobilized cells. (C) 2009 Elsevier B. V. All rights reserved.

    ELSEVIER SCIENCE SA, 2009年09月, BIOCHEMICAL ENGINEERING JOURNAL, 46 (1), 65 - 68, 英語

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    研究論文(学術雑誌)

  • Kazutaka Ikeda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

    A single-chain variable fragment (scFv) antibody against bisphenol A was refolded using an antigen (bisphenol A)-coupled column. The refolding efficiency was compared with that used in dialysis. The refolding efficiency of the antigen-coupled column was about 50-60%, which was much higher than with dialysis, due to a decrease in the concentration of the refolding molecules and to the suppression of the aggregate formation. (C) 2009 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2009年05月, BIOCHEMICAL ENGINEERING JOURNAL, 44 (2-3), 289 - 291, 英語

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    研究論文(学術雑誌)

  • Kentaro Yamada, Naoya Morishita, Tomohisa Katsuda, Shuji Kubo, Akinobu Gotoh, Hideki Yamaji

    The use of low-multiplicity infection of 293 cells in static culture with regular medium replacement was investigated for efficient large-scale production of adenovirus vectors for gene therapy applications. An adenovirus vector carrying the enhanced green fluorescent protein gene (Ad EGFP) was used to infect 293-F cells at a low multiplicity of infection (MOI) of 0.00001-0.1 transductional unit (TU) cell(-1). The cells, which have the ability to grow in suspension, were incubated in T-flasks and the serum-free culture medium was replaced with fresh medium via centrifugation every 2 days. Because only a small proportion of cells were initially infected at low MOIs (< 1 TU cell(-1)), uninfected cells continued to grow until they were infected by progeny adenoviruses released from previously infected cells. When 293-F cells at a relatively low density of 1 x 10(5) cells cm(-3) were infected with Ad EGFP at a low MOI of 0.001 TU cell(-1), the vector yield was 2.7-fold higher than the maximum yield obtained with high-multiplicity infection (MOI = 10 TU cell(-1)) in batch culture. These results indicate that efficient adenovirus vector production using low MOIs is achieved by minimization of either nutrient depletion and/or accumulation of inhibitory metabolites in the culture medium.

    SPRINGER, 2009年04月, CYTOTECHNOLOGY, 59 (3), 153 - 160, 英語

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    研究論文(学術雑誌)

  • Hasan M. Fida, Yoichi Kumada, Masaaki Terashima, Tomohisa Katsuda, Shigeo Katoh

    It is common for small tandem peptide multimer genes to be indirectly inserted into expression vectors and fused with a protein tag. In this study, a multimer of the tandem angiotensin I-converting enzyme inhibitory peptide (ACE-IP) gene was directly transferred to a commercially available vector and the designed gene was expressed as a repeated peptide in Escherichia coli BL21(DE3)pLysS. The process further developed in our study was the construction of six-repeated ACE-IP synthetic genes and their direct insertion. Protein expression in inclusion bodies was confirmed by SDS-PAGE and Western blot. Acid hydrolysis of inclusion bodies produced single-unit peptides through cleavage of the aspartyl-prolyl bonds. This cleaved recombinant peptide (rACE-IP) was purified using immuno-affinity chromatography followed by reversed phase-HPLC. 105-115 mg of the lyophilized recombinant peptide was obtained from 1 L E. coli culture. In vitro biological activity of rACE-IP was indistinguishable from that of the natural peptide produced by hydrolysis in artificial gastric juice or by acidic hydrolysis. The rACE-IP prepared by recombinant DNA technology and solid-phase synthesis methods showed a similar IC 50. This strategy could be used for the expression of important peptides, which have N-terminal proline (P) and C-terminal aspartic acid residues (D) for commercial applications, e.g. functional foods and drinks. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

    2009年, Biotechnology Journal, 4 (9), 1345 - 1356, 英語

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    研究論文(学術雑誌)

  • Yuka Kobayashi, Tadashi Yamauchi, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

    Angiotensin-1 converting enzyme (ACE) plays important roles in the regulation of blood pressure, and ACE inhibitory peptides in food materials have attracted attention for their antihypertensive function. In this study, the function of amino acids in ACE inhibitory tripeptides was clarified.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2008年09月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106 (3), 310 - 312, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Reza Ranjbar, Ryota Inoue, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

    In photobioreactors, photosynthetic microorganisms are exposed to certain light/dark cycles caused by light intensity distribution and mixing inside the photobioreactor. In this study Haematococcus pluvialis was cultivated in an airlift and a bubble column photobioreactor, and the cell growth and astaxanthin production were compared to clarify the effects of liquid circulation.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2008年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106 (2), 204 - 207, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroshi Suzuki, Jun-ya Hamamura, Tomohisa Katsuda, Yoshiyuki Komoda, Sigeo Katoh, Hiromoto Usui

    The characteristics of liposome formation in a micro-tube have been studied to develop a novel production method that forms the liposomes with a desired size homogeneously. In this method, the inner wall of the micro-tube was used to spread the liposomal lipid on. Then, instead of conventional rigorous agitation, an aqueous solution was fed through the tube, which peeled off the thin lipid layer formed liposomes. This resulted in a sharp peak in the size-distribution profile, and the size of liposomes was found to be dependent on the flow velocity anti on the micro-tube size. Moreover, the Yield of the liposomes of the desired size in our method was much higher than that in the conventional method, because our method produced liposomes uniform in size. From these findings, we concluded that the friction drag in the micro-tube is applicable for the efficient production of liposomes and a microfluidics device consisting of micro-tubes is a promising tool in the production of liposomal formulations.

    SOC CHEMICAL ENG JAPAN, 2008年08月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 41 (8), 739 - 743, 英語

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    研究論文(学術雑誌)

  • Reza Ranjbar, Ryota Inoue, Hironori Shiraishi, Tomohisa Katsuda, Shigeo Katoh

    Haematococcus pluvialis was cultivated under photoautotrophic conditions in a bubble column with fed-batch addition of nutrients, especially nitrate, and a cell number above 5 x 106 cells mL(-1) was attained after 300 h. The reduction of nutrient concentrations accompanied by dilution of the fermentation broth and an increase in the light intensity enhanced accumulation of astaxanthin. The final astaxanthin concentration of 390 mg L-1 was several times higher than ever reported. This combination of fed-batch addition of nutrients and dilution of broth for nutrient deficiency is a promising method for attainment of high cell and astaxanthin concentrations in a bubble column photobioreactor. (c) 2007 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE SA, 2008年05月, BIOCHEMICAL ENGINEERING JOURNAL, 39 (3), 575 - 580, 英語

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    研究論文(学術雑誌)

  • Tomohisa Katsuda, Hironori Shiraishi, Naoki Ishizu, Reza Ranjbar, Shigeo Katoh

    Flashing light from blue light emitting diodes is an effective method for the reduction of energy consumption in the bioproduction of astaxanthin by Haematococcus pluvialis. We investigated the effects of light intensity and frequency on the final astaxanthin concentration in bioproduction by H. pluvialis grown mixotrophically. The final astaxanthin concentration under illumination with flashing light, with frequencies ranging from 25 to 200 Hz, was dependent on the light intensity and on the duty cycle and was equivalent, or higher, in comparison with that under illumination with continuous light at the same incident intensity. The light intensity determined the maximum attainable concentration of astaxanthin under continuous illumination. Under illumination with flashing light, the ratio of the final astaxanthin concentration to the maximum concentration at a specific light intensity was correlated to the duty cycle in the frequency range from 25 to 200 Hz. The effect of lower frequencies on enhanced astaxanthin production under flashing light was also studied; at levels as low as I Hz, higher final astaxanthin concentrations were observed under flashing light compared to concentrations attained under continuous light.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2008年03月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105 (3), 216 - 220, 英語

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    研究論文(学術雑誌)

  • フォトバイオリアクターによる有用物質生産のための生物化学工学的検討

    勝田 知尚

    2008年, 生物工学会誌, Vo. 86, No. 3, pp. 117-122, 日本語

    研究論文(学術雑誌)

  • Shinya Yamawaki, Takehiro Matsumoto, Yuka Ohnishi, Yoichi Kumada, Naofumi Shiomi, Tomohisa Katsuda, Eun Kyu Lee, Shigeo Katoh

    This study examined the effects of two methods of methanol feeding, DO-stat and methanol concentration control, in fed-batch and continuous cultures of Pichia pastoris on cell growth and single-chain variable fragment antibody (scFv) expression. By maintaining the methanol concentration at 3.9 g l-1 in fed-batch culture, a scFv concentration of 198 mg l-1 was obtained. In continuous culture using both methanol feeding methods, the scFv concentration in the fermentation broth increased with a decreasing dilution rate. A maximum scFv concentration of 810 mg l-1 at a dilution rate of 0.0094 h-1 was obtained by maintaining the methanol concentration at 3.9 g l-1. Although the specific methanol consumption rate was the same for both methods, the specific productivity of scFv was higher in methanol concentration control from 0.0094 to 0.049 h-1 than it was in DO-stat control. Therefore, continuous culture with methanol feeding by the concentration control method shows promise for the industrial scale production of recombinant proteins by Pichia pastoris. © 2007 The Society for Biotechnology, Japan.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2007年11月, Journal of Bioscience and Bioengineering, 104 (5), 403 - 407, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Shigeo Katoh, Masami Imada, Naoki Takeda, Tomohisa Katsuda, Hiroyoshi Miyahara, Masaki Inoue, Shuji Nakamura

    Considering the large molecular size of IgG antibodies widely used for therapeutic applications, the pore size, pore volume and coupling density of silica-based media were optimized for the effective large-scale purification, using an antibody-protein A affinity system. Silica media, with average pore sizes from 70 nm to 140 nm and surface areas of 26-67 m(2)/g, were prepared and coupled with protein A. The static adsorption capacity and dynamic binding capacity of bovine and human IgG were measured at superficial liquid velocities ranging from 94 to 720 cm/h. The volumetric coefficient of mass transfer of the alkali-treated silica-based protein A media, with a pore size of 110nm, was four times higher than the values for cross-linked agarose media and thus had high dynamic binding capacities at high superficial liquid velocities. (c) 2007 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2007年08月, JOURNAL OF CHROMATOGRAPHY A, 1161 (1-2), 36 - 40, 英語

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    研究論文(学術雑誌)

  • Md. Fida Hasan, Yuka Kobayashi, Yoichi Kumada, Tomohisa Katsuda, Masaaki Terashima, Shigeo Katoh

    Inhibition characteristics of tri-peptides IKW and IKY, which were obtained as angiotensin-I converting enzyme inhibitors from bonito protein, and their stability in digestive canal were studied. These IKW and IKY showed high inhibitory activities with IC50 of 0.4 and 1.0 mu M, respectively, and acted as competitive inhibitors. The tri-peptides, especially IKY, were very stable under simulated gastrointestinal digestion conditions.

    SOC CHEMICAL ENG JAPAN, 2007年01月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 40 (1), 59 - 62, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Hiroyuki Yamamoto, Keishi Hada, Hideki Yamaji, Tomohisa Katsuda, Hiromu Ohno, Hideki Fukuda

    The analysis of data from analytical equipment will be an important factor in the execution of metabolomics. Self-modeling curve resolution (SMCR) is one of the theoretical techniques of chemometrics and has recently been applied to the data of hyphenated chromatography techniques. Alternating least squares (ALS) is a classical SMCR method. In ALS, however, different solutions are produced depending on randomly chosen initial values. Simulation in the present study showed that the use of a normalized constraint in calculating ALS was effective in avoiding this problem. We also improved the ALS algorithm by adding a regularized term (regularized ALS: RALS). Independent component analysis (ICA) is a comparatively new method and has been discussed very actively by information science researchers, but has still been applied only in very few cases to curve resolution problems in chemometrics studies. We applied RALS with a normalized constraint and ICA to the HPLC-DAD data of Haematococcus pluvialis metabolites and obtained a high accuracy of peak detection, suggesting that these curve resolution methods are useful for identification of metabolites in metabolomics. © 2006 Elsevier B.V. All rights reserved.

    ELSEVIER, 2006年12月01日, Biochemical Engineering Journal, 32 (3), 149 - 156, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomohisa Katsuda, Kazumichi Shimahara, Hironori Shiraishi, Keisuke Yamagami, Reza Ranjbar, Shigeo Katoh

    To conserve energy in the production of astaxanthin by the green alga Haematococcus pluvialis, we utilized intermittent flashing light from blue light emitting diodes (LEDs) and investigated the effects of the incident light intensity (2-12 mu mol m(-2) s(-1)), duty cycle (17-67%) and frequency (25-200 Hz) of flashing on the cell growth and astaxanthin production. In the above ranges, the final astaxanthin concentration under illumination by flashing light was significantly higher than that obtained under illumination with continuous light at the same incident intensity. For example, flashing light at an incident intensity of 8 mu mol m(-2) s(-1) gave the same final astaxanthin concentration that was obtained under continuous light illumination at 12 mu mol m(-2) s(-1), thus reducing energy consumption by 1/3. We therefore conclude that flashing light from blue LEDs is a promising illumination method for indoor algal cultivation using photobioreactors.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2006年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102 (5), 442 - 446, 英語

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    研究論文(学術雑誌)

  • Tomohisa Katsuda, Mitsumasa Kamura, Yasushi Nishiwada, Shigeo Katoh

    A single nucleotide polymorphism (SNP) was detected by the use of affinity chromatography with a single-stranded DNA ligand and temperature gradient elution. Because of the difference in thermostability between the ligand and sample single-stranded DNAs with and without SNPs, they were eluted as a peak with a shoulder by the temperature gradient elution. The sample DNAs with SNPs were detected as low as 0.1 pmol, and the elution behavior of DNAs with and without SNPs in this chromatography was predicted by a numerical calculation method based on a thermodynamic mass transfer model.

    SOC CHEMICAL ENG JAPAN, 2006年10月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 39 (10), 1104 - 1107, 英語

    [査読有り]

    研究論文(学術雑誌)

  • KATSUDA Tomohisa, OOSHIMA Hiroshi, AZUMA Masayuki, KATO Jyoji

    A water-soluble color indicator was developed for the effective screening of hydrogen-producing microorganisms. This indicator consists of a coloring agent and a water-soluble derivative of Wilkinson's catalyst. Wilkinson's catalyst, Tris(triphenylphosphine) rhodium chloride, had been developed as a catalyst for the hydrogenation of olefins. We used a sulfonate of the catalyst for the hydrogenation of coloring agent in an aqueous medium. Several coloring agents, such as methyl orange, methyl red sodium, neutral red and Evan's blue, dissolved in water together with the sulfonated catalyst showed a change in color when hydrogen gas was fed into the solution by sparging at room temperature. We confirmed that methyl orange was decolorized by biologically produced hydrogen, when the photosynthetic bacterial strain Rhodobacter capsulatus ST-410 was grown in a medium containing 0.6 mM catalyst and 0.075 mM methyl orange in test tubes of 5 ml working volume.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2006年09月, J. Biosci. Bioeng., 102 (3), 220 - 226, 英語

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    研究論文(学術雑誌)

  • Naofumi Shiomi, Yutaka Yamaguchi, Hiroaki Nakai, Tomoko Fujita, Tomohisa Katsuda, Shigeo Katoh

    The rates of degradation of cyanuric acid, a key intermediate in a metabolic pathway of s-triazine herbicides, were measured for Pseudomonas sp. NRRL B-12227. The rate of degradation was affected by the rate of cyanuric acid transport through cell membranes and the activity of cyanuric acid amidohydrolase inside the cells. At low concentrations of cyanuric acid, the acclimation of cells to cyanuric acid and/or added nutrients effectively enhanced the degradation rate. The strain was also applied to bioremediation using a Bioremediation with Self-Immobilization System (BSIS), in which Pseudomonas sp. NRRL B-12227 cells were co-immobilized with Bacillus subtilis, the latter of which secretes a viscous polymer, in a shallow layer of soil packed in a column. More than 70% of the Pseudomonas sp. NRRL B-12227 cells were co-immobilized with the B. subtilis in a 7.5 cm layer of the packed soil by self-aggregation. More than 60% of the 1 mM cyanuric acid supplied to the packed soil was degraded in this layer during a 72 h period.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2006年09月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102 (3), 206 - 209, 英語

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    研究論文(学術雑誌)

  • Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Yasushi Nishiwada, Shigeo Katoh

    We developed an affinity chromatographic method for simple single nucleotide polymorphism (SNP) detection by use of a single-stranded DNA-coupled column and temperature gradient elution, utilizing the difference in thermal stability between hybridized double-stranded DNAs with and without mismatched base-pairs in the course of temperature gradient elution. We studied experimentally and theoretically the elution behavior of DNAs with and without SNPs in this chromatography and proposed a numerical calculation method based on a thermodynamic dissociation model. The effects of the column volume, flow rate of eluent and heating rate of the column on elution profiles were clarified. For designing DNA ligands, mismatched base-pair positions favorable for detection of SNPs were also explored by use of hybridized DNAs coding a part of the human TP53 gene. (c) 2006 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, 2006年08月, JOURNAL OF CHROMATOGRAPHY A, 1123 (2), 182 - 188, 英語

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    研究論文(学術雑誌)

  • Satoshi Yoshimura, Reza Ranjbar, Ryota Inoue, Tomohisa Katsuda, Shigeo Katoh

    A new cultivation method, in which physiological responses of Haematococcus pluvialis to different intensities and wavelengths of illuminating light are utilized, is proposed. In this method, light transmitted through a cultivation vessel illuminated from the opposite side was utilized for the early-phase cultivation of H. pluvialis in another inoculated vessel that was located behind the cultivation vessel, to save time required for the growth of cells. After harvesting cells from the front vessel, the vessel that was originally behind was shifted to the position of the front vessel. The abrupt increase in light intensity caused by shift of the position induced the effective accumulation of astaxanthin. These procedures for inoculation, shift of vessels and harvest were repeated using two vessels arranged in series along the light path. After four repeated cycles of cultivation (840 h from the start of cultivation), using 2.5 cm thick vessels, astaxanthin production per unit volume and the astaxanthin content were higher than obtained in a batch cultivation with two 2.5 cm vessels.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2006年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102 (2), 97 - 101, 英語

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    研究論文(学術雑誌)

  • F. Hasan, Y. Kumada, N. Hashimoto, T. Katsuda, M. Terashima, S. Katoh

    Kinetics of fragmentation of angiotensin-I converting enzyme inhibitory peptides obtained by digestion in gastric juice were studied under intestinal digestion conditions and their inhibitory activities were determined. A fragment IKYGD produced by digestion, as well as IKWGD synthesized, showed similar inhibitory activity to the original peptides. These peptides somehow were resistant to tryptic and/or chymotryptic digestion, and IK + aromatic amino acid might be important functional parts in some kinds of ACE inhibitory peptides.

    INST CHEMICAL ENGINEERS, 2006年06月, FOOD AND BIOPRODUCTS PROCESSING, 84 (C2), 135 - 138, 英語

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    研究論文(学術雑誌)

  • R Yegani, S Yoshimura, K Moriya, T Katsuda, S Katoh

    Using a semicontinuous culture method, in which operational parameters such as cell concentration and light intensity distribution were maintained almost constant, instability of the specific growth rate of Rhodobacter capsulatus B-100, a purple bacterium, was observed to be similar to that of R. capsulatus ST-410 when cultivated under high ratios of light intensity on the illuminated side to that of the transmitted light. Such instability was not observed in the cultivation of Chlorella vulgaris, a eukaryotic green alga, even at higher cell concentrations. Under the same conditions, the increase in only the ferrous concentration from 43 mu M, the concentration in the original RCV medium, to 172 mu M sustained a stable growth, whereas Fe2+ was slightly consumed during the cultivation. Supplemental illumination with a fluorescent lamp on the transmitted side of a flat plate photobioreactor sustained a moderate level of stable growth, while a halogen lamp slightly affected the growth stability. Our results showed that an increase in Fe2+ concentration or supplemental illumination improves the growth stability of R. capsulatus.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2005年12月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100 (6), 672 - 677, 英語

    [査読有り]

    研究論文(学術雑誌)

  • A Lababpour, K Shimahara, K Hada, Y Kyoui, T Katsuda, S Katoh

    To increase the cell concentration and the accumulation of astaxanthin, the effects of the fed-batch addition of 10-fold-concentrated medium to supply nutrients, as well as illumination with blue light emitting diodes (LEDs), on cell growth and accumulation of astaxanthin were studied for the cultivation of Haematococcus pluvialis. Using the fed-batch addition method, the cell concentration increased above 1 mg-dry cell/cm(3), and under illumination with blue LEDs, the astaxanthin concentration reached approximately 70 mu g/cm(3). This method was much simpler to operate than the medium replacement method in operation and enabled us to attain a higher total yield of astaxanthin.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2005年09月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 100 (3), 339 - 342, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Semi-Continuous Cultivation of Photosynthetic Cells in Flat Plate Photobioreactor

    YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo

    2005年, Iranian Journal of Chemical Engineering, 2, 15-21, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Neutralization of acids by microorganisms co-immobilized with Bacillus subtilis in a shallow layer of model soil

    T Yasuda, N Shiomi, S Iwasaki, Y Yamaguchi, T Katsuda, S Katoh

    Fungus and bacterium, which could grow under acidic conditions, were isolated, and their characteristics of growth and neutralization of acids were studied. They could grow in acidic media (pH 4.0) containing several kinds of acids at the same rate in the medium of pH 7.2 and neutralize acids. For bioremediation of pollutants in soil, we proposed a self-immobilization method of microorganisms forming aggregates in a shallow layer of soil. These isolated strains, which showed weak aggregation and were different in size, were successfully co-immobilized with Bacillus subtilis secreting viscous polymer in a shallow layer of model soil packed in a column and could neutralize acidic solutions flowing through packed soil.

    SOC CHEMICAL ENG JAPAN, 2004年12月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 37 (12), 1445 - 1451, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Effects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis

    A Lababpour, K Hada, K Shimahara, T Katsuda, S Katoh

    In order to increase the cell concentration and the accumulation of astaxanthin, the effects of nutrient concentration, pH, illumination and methods of supplying nutrients were studied for the cultivation of Haematococcuspluvialis. The replacement of media to avoid the deficiency of nutrients increased the cell concentration above 1 mg-dry cell cm(-1) without induction of astaxanthin accumulation. Illumination with blue light emitting diode lamps and nutrient starvation induced accumulation of astaxanthin, and the interactive effects of these two increased the astaxanthin concentration to 76 mug cm(-3).

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2004年12月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 98 (6), 452 - 456, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T Katsuda, A Lababpour, K Shimahara, S Katoh

    The photosynthetic microalga Haematococcus pluvialis, a potential source of astaxanthin, was cultivated under illumination with LEDs emitting red (lambda(max) = 625 nm), green(lambda(max) = 525 nm), blue (lambda(max) = 470 nm), blue-purple (lambda(max) = 410 nm) and purple (lambda(max) = 380 nm) light and a fluorescent lamp, and the effects of wavelength on cell growth and astaxanthin accumulation were studied. LEDs emitting light of short wavelengths (380-470 nm) were found to induce astaxanthin accumulation of up to 5-6% per dry cell, although the induction caused the suppression of cell growth. From these results, we proposed a new strategy of cultivating H. pluvialis under illumination with red LEDs without inducing a high level of astaxanthin accumulation, and then switching to illumination with blue LEDs at a high light intensity to induce a high level of astaxanthin accumulation. (C) 2004 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, 2004年07月, ENZYME AND MICROBIAL TECHNOLOGY, 35 (1), 81 - 86, 英語

    [査読有り]

    研究論文(学術雑誌)

  • T Katsuda, R Yegani, N Fujii, K Igarashi, S Yoshimura, S Katoh

    For cultivation of photosynthetic cells under defined light intensity distributions, the repeated batch culture, in which a part of culture broth containing grown cells was repeatedly replaced at predetermined time intervals with a fresh medium to keep the cell concentration constant at an initial value, was employed. By use of this method the effects of the light intensity distribution on the growth characteristics of Rhodobacter capsulatus were studied. Unexpected decreases in the specific growth rate were observed in culture of R. capsulatus at high cell concentrations and a long light path length. Big differences in the light intensities of lightly and darkly illuminated portions in photobioreactors, which reflects the light intensity distribution, seemed to cause this phenomenon, which must be taken into consideration for stable growth of photosynthetic cells.

    AMER CHEMICAL SOC, 2004年05月, BIOTECHNOLOGY PROGRESS, 20 (3), 998 - 1000, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Characteristics of neutralization of acids by newly isolated fungal cells

    N Shiomi, T Yasuda, Y Inoue, N Kusumoto, S Iwasaki, T Katsuda, S Katoh

    Soil microorganisms play an important role in maintaining soil pH at levels suitable for other soil organisms. To clarify the biological neutralization mechanism in soil, we isolated soil microorganisms showing a high ability to neutralize acids and studied their characteristics. From our taxonomic study, three isolated strains were identified as filamentous fungi, namely Mucor sp., Aspergillus fumigatus, and Aureobasidium pullulans. These strains could secrete basic materials, such as ammonia, for neutralization, grow in the medium at pH 4.0 and increase the pH of the medium to approximately 8.0. These microbial cells could neutralize not only nitric acid but also sulfuric and hydrochloric acids. The strains could also grow by utilizing nitric acid as a sole nitrogen source. In the soil containing these organisms, the pH was maintained in the neutral range by the buffering action of basic materials that they secrete. These results suggest that these fungal cells are useful for protecting the soil from acidification by acid rain.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2004年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97 (1), 54 - 58, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Relationship between Light Intensity Distribution and Growth Rate of Photosynthetic Cells

    YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo

    2004年, Conference Proceedings of 10th The APPhE Congress, 3P-01-085, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Effects of Medium Replacement on Cell Density and Astaxanthin Accumulation in H. pluvialis Illumination by LED Lamps

    Abdolmajid Lababpour, HADA Keishi, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo

    2004年, Conference Proceedings of 10th The APPhE Congress, 3L-07, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Detection of SNPs Using Oligonucleotide Immobilized Capillary Chromatography

    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, Zakir Hossain, KATOH Shigeo

    2004年, Conference Proceedings of 10th The APPhE Congress, 1P-01-009, 英語

    [査読有り]

    研究論文(国際会議プロシーディングス)

  • Mahmoud Farshbaf, Yoshihiro Katoh, Taro Udaka, Tomohisa Katsuda, Hideo Noda, Shigeo Katoh

    Taylor-eddy and paddle type apparatus were applied for refolding of lysozyme in a continuous system. The number of theoretical mixing stages was measured for both the apparatus and the effect of flow rate and mixing rate on the axial dispersion of solutions flowing inside of a ceramic membrane tube were investigated. Refolding at different concentrations of lysozyme was performed by addition of denatured lysozyme into refolding buffers flowing through the inside of the tube. For studying the effect of addition, different addition methods of denatured lysozyme into refolding buffer were devised by using ceramic membranes, from the total surface of the membrane tube and partially upside ceramic membrane. Comparing the efficiencies of continuous refolding with those of batch method, higher refolding efficiencies were obtained in the continuous system at the same concentrations of lysozyme and urea. Therefore, by combining the merits of the fed-batch method and continuous system, the refolding efficiency can be improved as well as throughput of refolding process.

    2002年10月, Journal of Chemical Engineering of Japan, 35 (10), 963 - 968, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Light attenuation in suspension of the purple bacterium Rhodobacter capsulatus and the green alga Chlamydomonas reinhardtii

    T Katsuda, N Fujii, N Takata, H Ooshima, S Katoh

    A simple and rapid calculation method, which is based on the Beer-Lambert law and Rose and Lloyd's hypothesis, was proposed for the determination of light attenuation profiles in suspensions of photosynthetic microorganisms. In this method, extinction coefficients measured spectrophotometrically at 2 single concentration of suspended cells over the whole range of wavelengths emitted from different light sources were used for reflecting the physical and biological characteristics of cells, which may change during a course of cultivation. The validity of this approach was confirmed by use of suspensions of two kinds of photosynthetic cells, the purple bacterium Rhodobacter capsulatus and the green alga Chlamydomonas reinhardtii, illuminated by three light sources with different irradiation spectra. This approach can estimate well the profiles of the light attenuation in these systems, irrespective of the spectra of the light sources, until the transmitted light intensity falls to one tenth of the incident intensity.

    SOC CHEMICAL ENG JAPAN, 2002年05月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 35 (5), 428 - 435, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Control of aggregate formation in refolding of carbonic anhydrase at high urea concentrations and effects of urea removal

    M Farshbaf, Y Katoh, T Morimoto, T Udaka, T Katsuda, S Katoh

    Refolding of CAB was studied at high concentrations of protein and urea. Aggregate formation was decreased by using higher concentrations of urea in refolding buffer. Refolding yields of 60% were obtained at high concentrations of CAB by using urea concentrations of 3.0-4.0 M in renaturation mixture, Comparison of the refolding yield and the amount of soluble CAB in renaturation mixture indicated that the apparent specific activity of refolded CAB was lower than that of native one at higher concentrations of urea. Difference in the steric structures of native and refolded CAB was observed in CD spectra of these samples. However, removal of urea from loosely folded CAB solution by dialysis recovered the native structure of CAB, as well as its native specific activity. Hence the refolding efficiency after dialysis was improved to about 70-80% at CAB concentrations of 3-5 kg/m(3). Effect of incubation time of renaturation mixture before dialysis was studied, and it was shown that incubation time less than 3 hours increased aggregate formation and decreased the refolding yield. Therefore, formation of more stable intermediates in the refolding pathway decreases aggregate formation during urea removal. These results lead to a consecutive dilution-dialysis method for refolding at high concentrations of proteins.

    SOC CHEMICAL ENG JAPAN, 2001年06月, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 34 (6), 743 - 747, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomohisa Katsuda, Takeshi Arimoto, Koichi Igarashi, Masayuki Azuma, Jyoji Kato, Susumu Takakuwa, Hiroshi Ooshima

    The light distribution in the externally illuminated cylindrical photo- bioreactor for production of hydrogen by a photosynthetic bacterium Rhodobacter capsulatus ST-410 was estimated. The estimation was performed on the basis of the Matsuura and Smith's diffuse model [1]. In the diffuse model, the incident light rays are assumed to proceed in every direction and the local intensity is calculated as the sum of the intensities of light. Since Lambert-Beer's law, extensively used in photometry, was not useful for explaining the decrease in the intensity of light by the biomass, an empirical expression was used. The measurement of the intensities from every direction was conducted in an externally illuminated cylindrical photo- bioreactor having an inner diameter of 60 mm and a working volume of 550 ml. The obtained results confirmed our estimation. The light distribution was applied to estimate the hydrogen production by R. capsulatus ST-410 using the same photo-bioreactor. The overall hydrogen-production rate was successfully estimated. (C) 2000 Elsevier Science S.A.

    2000年06月, Biochemical Engineering Journal, 5 (2), 157 - 164, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Tomohisa Katsuda, Masayuki Azuma, Jyoji Kato, Susumu Takakuwa, Hiroshi Ooshima

    Ethanolamine was examined as a nitrogen source in the production of hydrogen by Rhodobacter capsulatus ST-410, a hydrogenase-deficient mutant of the strain B-100. It was found that ethanolamine supports cell growth as the sole nitrogen source and permits a large amount of hydrogen evolution, detected at 138 μmol/ml- culture from 3.5 mM ethanolamine and 30 mM DL-malate. The amount corresponded to a stoichiometric yield of 77% and was close to that obtained from 7.0 mM L-glutamate and 30 mM DL-malate. The hydrogen evolution rate per unit biomass (cells) was higher than that with L-glutamate, and the cells grown with ethanolamine had higher nitrogenase activity than the cells grown with L-glutamate. In terms of bioconversion of cellulosic and hemicellulosic biomass to hydrogen, D-glucose, D-xylose, and D-cellobiose were tested as substrates. The results indicated that those sugars permit a large evolution of hydrogen through cultivation with ethanolamine as a nitrogen source. For instance, the cells grown with 3.5 mM ethanolamine evolved hydrogen of 289 μmol/ml-culture (80% yield) from 30 mM D-glucose under a controlled pH of 6.4 to 6.9. © 2000, Taylor & Francis Group, LLC. All rights reserved.

    2000年01月01日, Bioscience, Biotechnology and Biochemistry, 64 (2), 248 - 253, 英語

    [査読有り]

    研究論文(学術雑誌)

  • 澤本 詩織, 鈴木 輔, 古田 貴憲, 小川 隆文, 勝田 知尚, 山地 秀樹

    公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 1067 - 1067, 日本語

  • Ryou Nakanuma, Kyoko Masumi-Koizumi, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji

    Insect cells have recently proven to be an excellent platform for the high-level production of functional recombinant proteins. Autophagy is an important mechanism that promotes cell survival by eliminating damaged organelles and protein aggregates, and it also may influence recombinant protein production. In the present study, we compared the effects that autophagy inducers rapamycin, everolimus, and lithium chloride exert on recombinant lepidopteran insect cells that secrete an engineered antibody molecule. Compared with nontreatment, treatment with either rapamycin or everolimus prolonged cell growth to allow high cell density, improved viability in the declining phase, and then increased the yield of secreted antibodies. These positive effects appeared to be induced via autophagy since autophagosomes were clearly detected, particularly in cells treated with rapamycin or everolimus. Unlike rapamycin, another autophagy inducer, FK506, was ineffective in insect cells. The addition of an appropriate autophagy inducer may be effective in increasing the productivity of recombinant proteins in insect cells.

    SPRINGER, 2021年06月, CYTOTECHNOLOGY, 73 (3), 299 - 305, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

    2021年01月, MATEC Web of Conferences, 333, 07009 - 07009, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

    Virus-like particles (VLPs) are hollow nanoparticles composed of recombinant viral surface proteins without a virus genome. In the present study, we investigated the production of influenza VLPs using recombinant insect cells. DNA fragments encoding influenza A virus hemagglutinin (HA) and matrix protein 1 (M1) were cloned with the Drosophila BiP signal sequence in plasmid vectors containing a blasticidin and a neomycin resistance gene, respectively. After Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were co-transfected with a pair of constructed plasmid vectors, stably transformed cells were established via incubation with blasticidin and G418. Western blot analyses showed that recombinant High Five cells secreted HA and M1 proteins into the culture supernatant. Immunoprecipitation of the culture supernatant with an anti-HA antibody and transmission electron microscopy suggested that secreted HA and M1 proteins were in a particulate structure with a morphology similar to that of an influenza virus. Hemagglutination assay indicated that expressed HA molecules retained hemagglutination activity. In a shake-flask culture, recombinant cells achieved a high HA yield (approximate to 10 mu g/ml) comparable to the yields obtained using the baculovirus-insect cell system. Recombinant insect cells may serve as excellent platforms for the efficient production of influenza VLPs for use as safe and effective vaccines and diagnostic antigens.

    ELSEVIER, 2020年11月, BIOCHEMICAL ENGINEERING JOURNAL, 163, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Yu Mizote, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

    Antibody Fab fragments consist of heavy chain (Hc) and light chain (Lc) polypeptides assembled with a disulphide bond. The production of a recombinant Fab fragment requires the simultaneous expression of two genes encoding both an Hc and an Lc in the same host cell. In the present study, we investigated the production of Fab fragments in lepidopteran insect cells using a bicistronic plasmid vector carrying the Hc and Lc genes linked with a 2A self-cleaving peptide sequence from the porcine teschovirus-1. We also examined the arrangement of a GSG spacer sequence and a furin cleavage site sequence with the 2A sequence. Western blot analysis and enzyme-linked immunosorbent assay (ELISA) of culture supernatants showed that Trichoplusia ni BTI-TN-5B1-4 (High Five) cells transfected with a plasmid in which the Hc and Lc genes were joined by the 2A sequence successfully secreted Fab fragments with antigen-binding activity after self-cleavage of the 2A peptide. The GSG linker enhanced 2A cleavage efficiency, and the furin recognition site was useful for removal of 2A residues from the Hc. Transfection with a single plasmid that contained sequences for GSG, the furin cleavage site, GSG, and the 2A peptide between the Hc and Lc genes exhibited a higher productivity than co-transfection with a set of plasmids separately carrying the Hc or Lc gene. These results demonstrate that bicistronic expression with the appropriate combination of a furin recognition site, GSG linkers, and a 2A peptide may be an effective way to efficiently produce recombinant antibody molecules in insect cells. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2020年08月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 130 (2), 205 - 211, 英語

    [査読有り]

    研究論文(学術雑誌)

  • Production of influenza virus-like particles in insect cells

    Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda

    2020年05月, BMC Proceedings, 14, 14 - 15, 英語

    研究論文(国際会議プロシーディングス)

  • Hiroshi Ooshima, Susumu Takakuwa, Tomohisa Katsuda, Masaki Okuda, Takeshi Shirasawa, Masayuki Azuma, Jyoji Kato

    Elsevier BV, 1998年01月, Journal of Fermentation and Bioengineering, 85 (5), 470 - 475, 英語

    [査読有り]

    研究論文(学術雑誌)

MISC

  • Atsushi Fujiwara, Hiroshi Suzuki, Tomohisa Katsuda, Yoshiyuki Komoda

    SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S22 - S22, 英語

    研究発表ペーパー・要旨(国際会議)

  • Tomohisa Katsuda, Chiaki Ogino, Keisuke Yamagami, Shigeo Katoh, Nobuaki Shimizu

    ELSEVIER SCIENCE BV, 2007年09月, JOURNAL OF BIOTECHNOLOGY, 131 (2), S185 - S186, 英語

    研究発表ペーパー・要旨(国際会議)

  • Growth stability of photosynthetic bacteria: Effects of supplemental illumination and ferrous ion concentration

    R Yegani, S Yoshimura, T Katsuda, S Katoh

    ELSEVIER SCIENCE BV, 2005年08月, JOURNAL OF BIOTECHNOLOGY, 118, S113 - S113, 英語

    研究発表ペーパー・要旨(国際会議)

  • Fed-batch cultivation of Haematococcus pluvialis under illumination with LEDs for production of astaxanthin

    A Lababpour, T Katsuda, S Katoh

    ELSEVIER SCIENCE BV, 2005年08月, JOURNAL OF BIOTECHNOLOGY, 118, S116 - S116, 英語

    研究発表ペーパー・要旨(国際会議)

  • 3Hp25 Computational fluid dynamics for enhancing the light distribution in a photobioreactor grown culture of Hematococcus pluvialis :

    Giannelli Luca, Wada Sotaro, Yamaji Hideki, Katsuda Tomohisa

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 157 - 157, 英語

  • 2Bp21 組換え昆虫細胞によるscFv-Fc融合タンパク質の生産(バイオプロセス/センサー,計測工学/セル&ティッシュエンジニアリング,一般講演)

    薗田 啓之, 熊田 陽一, 勝田 知尚, 山地 秀樹

    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 32 - 32, 日本語

書籍等出版物

  • Production of antibody fragments in Escherichia coli. In: Patrick Chames (ed.), Antibody Engineering: Methods and Protocols, Second Edition, Method in Molecular Biology, vol. 907, pp. 305-324 (Chapter 18)

    KATSUDA Tomohisa, SONODA Hiroyuki, KUMADA Yoichi, YAMAJI Hideki

    その他, Springer Science+Business Media, 2012年08月, 英語

    学術書

  • 抗体医薬のための細胞構築と培養技術

    勝田 知尚

    その他, シーエムシー出版, 2010年06月, 日本語

    学術書

  • エコバイオエネルギーの最前線

    勝田 知尚, 大嶋 寛

    共著, シーエムシー出版, 2005年09月, 日本語

    学術書

講演・口頭発表等

  • 固定化したシトクロムP450発現大腸菌によるwhole cell bioconversion

    藤井 敦士, 石坂 明穂, 勝田 知尚, 今石 浩正, 山地 秀樹

    化学工学会第84年会, 2019年03月, 日本語, 化学工学会, 東京, 国内会議

    ポスター発表

  • Production of influenza virus-like particles in stably transformed insect cells

    MATSUDA Takuya, TANIJIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

    31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), 2018年11月, 英語, 日本動物細胞工学会, 筑波, 国際会議

    ポスター発表

  • Production of antibody Fab fragment using 2A peptide in insect cells

    MIZOTE Yu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa, YAMAJI Hideki

    31st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), 2018年11月, 英語, 日本動物細胞工学会, 筑波, 国際会議

    ポスター発表

  • 緑藻Chlorella sorokinianaの光独立栄養培養における培養温度の影響

    桑井 仁葵, 山地 秀樹, 勝田 知尚

    化学工学会第50回秋季大会, 2018年09月, 日本語, 化学工学会, 鹿児島, 国内会議

    ポスター発表

  • 培養温度・光強度の周期的な変化が緑藻Haematococcus pluvialisの細胞分裂に及ぼす影響

    森本 高章, 山地 秀樹, 勝田 知尚

    化学工学会第50回秋季大会, 2018年09月, 日本語, 化学工学会, 鹿児島, 国内会議

    ポスター発表

  • 昆虫細胞における2Aペプチドを用いた抗体生産

    溝手 結, 増見 恭子, 勝田 知尚, 山地 秀樹

    第70回日本生物工学会大会, 2018年09月, 日本語, 日本生物工学会大会, 吹田, 国内会議

    口頭発表(一般)

  • ゲノム編集技術を用いた組換え昆虫細胞の作製

    戸上 真也, 増見 恭子, 勝田 知尚, 山地 秀樹

    化学工学会第50回秋季大会, 2018年09月, 英語, 化学工学会, 鹿児島, 国内会議

    ポスター発表

  • Production of influenza virus proteins in stably transformed insect cells

    YAMAJI Hideki, TANIJIMA Toshikazu, MATSUDA Takuya, Kyoko MASUMI-KOIZUMI, KATSUDA Tomohisa

    18th European Congress on Biotechnology (ECB2018), 2018年07月, 英語, European Federation of Biotechnology, Geneva, Switzerland, 国際会議

    ポスター発表

  • 緑藻Haematococcus pluvialisにおける周期的温度変化と細胞増殖の関係

    勝田 知尚, 森本 高章, 山地 秀樹

    化学工学会第83年会, 2018年03月, 日本語, 化学工学会, 吹田, 国内会議

    ポスター発表

  • 昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産

    松田 拓也, 谷島 寿和, 増見 恭子, 勝田 知尚, 山地 秀樹

    化学工学会第83年会, 2018年03月, 日本語, 化学工学会, 吹田, 国内会議

    ポスター発表

  • 昆虫細胞による2Aペプチドを用いた抗体タンパク質の生産

    溝手 結, 増見 恭子, 勝田 知尚, 山地 秀樹

    化学工学会第49回秋季大会, 2017年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • 共焦点レーザー走査顕微鏡観察と数値ミュレーションに基づく抗体の吸着・破過の解析

    廣岡 奏, YAMAGUCHI Tomoki, 山地 秀樹, 勝田 知尚

    化学工学会第49回秋季大会, 2017年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • エアリフト式フォトバイオリアクターの操作条件と微細藻類の光独立栄養増殖の関係

    紅林 愛夏, 森本 高章, 西村 拓海, 山地 秀樹, 勝田 知尚

    化学工学会第49回秋季大会, 2017年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • Production of influenza virus proteins by recombinant insect cells

    YAMAJI Hideki, TANISHIMA Toshikazu, MASUMI-KOIZUMI Kyoko, KATSUDA Tomohisa

    International Meeting & 42nd Annual Conference of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB), 2017年08月, 英語, The Malaysian Society for Biochemistry & Molecular Biology (MSBMB), Kuala Lumpur, Malaysia, 国際会議

    ポスター発表

  • 昆虫細胞を用いたインフルエンザウイルスタンパク質の生産

    山地 秀樹, 谷島 寿和, 増見 恭子, 勝田 知尚

    第30回日本動物細胞工学会2017年度大会 (JAACT2017), 2017年07月, 日本語, 日本動物細胞工学会, 松山, 国内会議

    ポスター発表

  • Efficient protein production by transient gene expression using insect cells

    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa

    25th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2017), 2017年05月, 英語, European Society for Animal Cell Technology (ESACT), Lausanne, Switzerland, 国際会議

    ポスター発表

  • Transient gene expression in insect cells for efficient recombinant antibody production

    TAKAHASHI Ryoma, MASUMI-KOIZUMI Kyoto, OHMURO-MATSUYAMA Yuki, KATSUDA Tomohisa, YAMAJI Hideki

    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), 2016年11月, 英語, 日本動物細胞工学会, 神戸, 国際会議

    ポスター発表

  • Production of virus-like particles using recombinant insect cells

    TANISHIMA Toshikazu, KATSUDA Tomohisa, YAMAJI Hideki

    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), 2016年11月, 英語, 日本動物細胞工学会, 神戸, 国際会議

    ポスター発表

  • Characterization of the diffusion and adsorption of IgG in protein A-coupled adsorbents with confocal laser scanning microscopy

    HIROOKA Kanamu, HARUNA Yusuke, YAMAJI Hideki, KATSUDA Tomohisa

    The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), 2016年11月, 英語, 日本動物細胞工学会, 神戸, 国際会議

    ポスター発表

  • Efficient antibody production by transient gene expression in insect cells

    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa

    17th European Biotechnology Congress (ECB2016), 2016年07月, 英語, European Federation of Biotechnology (EFB), Krakow, Poland, 国際会議

    ポスター発表

  • 培養条件の概日変化が緑藻Haematococcus pluvialisの栄養増殖に及ぼす影響

    吉峰 幸平, 山田 浩之, 勝田 知尚, 山地 秀樹

    化学工学会第81年会, 2016年03月, 日本語, 化学工学会, 大阪, 国内会議

    ポスター発表

  • 共焦点レーザー走査顕微鏡観察に基づくProtein A固定化吸着剤の拡散・吸着特性の評価

    勝田 知尚, 春名 祐佐, 廣岡 奏, 加瀬 裕貴, 大田 洸, 吉川 徹, 山地 秀樹

    化学工学会第81年会, 2016年03月, 日本語, 化学工学会, 大阪, 国内会議

    口頭発表(一般)

  • Recombinant antibody production by transient gene expression in insect cells

    YAMAJI Hideki, MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa

    Asian Congress on Biotechnology 2015 (ACB2015), 2015年11月, 英語, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, 国内会議

    ポスター発表

  • Photoautotrophic growth of Haematococcus pluvialis under controlled temperature and light conditions

    KATSUDA Tomohisa, GIANNELLI Luca, YAMADA Hiroyuki, YAMAJI Hideki

    Asian Congress on Biotechnology 2015 (ACB2015), 2015年11月, 英語, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, 国内会議

    ポスター発表

  • Optimization of microalgal cultivation in cascade photobioreactors with wavy bottom via computational fluid dynamics

    WATANABE Chihiro, GIANNELLI Luca, YAMAJI Hideki, KATSUDA Tomohisa

    Asian Congress on Biotechnology 2015 (ACB2015), 2015年11月, 英語, Asian Federation of Biotechnolgy (AFOB), Kuala Lumpur, Malaysia, 国内会議

    ポスター発表

  • シトクロムP450発現大腸菌を用いたwhole cell bioconversionに及ぼす培養・反応条件の影響

    原田 真行, 森中 涼平, 勝田 知尚, 今石 浩正, 山地 秀樹

    化学工学会第47回秋季大会, 2015年09月, 日本語, 化学工学会, 札幌, 国内会議

    ポスター発表

  • 昆虫細胞を宿主とした一過性発現による抗体タンパク質の生産

    森 慶太, 濱田 宏嗣, 大室 有紀, 勝田 知尚, 山地 秀樹

    第28回日本動物細胞工学会2015年度大会 (JAACT2015), 2015年07月, 日本語, 日本動物細胞工学会, 仙台, 国内会議

    ポスター発表

  • Production of an antibody molecule by transient gene expression in insect cells

    MORI Keita, HAMADA Hirotsugu, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki

    24th ESACT Meeting Barcelona 2015, 2015年06月, 英語, European Society for Animal Cell Technology (ESACT), Barcelona, Spain, 国際会議

    ポスター発表

  • 鉛に汚染された土壌の評価とバイオレメディエーションによる鉛の回収

    塩見 尚史, 末次 憲一郎, 勝田 知尚

    日本LCA学会, 2015年03月, 日本語, 国内会議

    口頭発表(一般)

  • カスケード型フォトバイオリアクターにおける微細藻類培養の数値流体力学に基づく検討

    渡邉 千尋, GIANNELLI Luca, 山地 秀樹, 勝田 知尚

    化学工学会姫路大会, 2014年12月, 日本語, 化学工学会関西支部, 姫路, 国内会議

    ポスター発表

  • Transient gene expression in insect cells for recombinant antibody production

    MORI Keita, HAMADA Hirotsugu, OGAWA Takahumi, OHMURO Yuki, KATSUDA Tomohisa, YAMAJI Hideki

    The 27th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT2014 Kitakyushu), 2014年11月, 英語, 日本動物細胞工学会, 北九州, 国際会議

    ポスター発表

  • 昆虫細胞を宿主とした一過性発現による抗体タンパク質生産

    濱田 宏嗣, 森 慶太, 小川 隆文, 大室 有紀, 勝田 知尚, 山地 秀樹

    化学工学会第46回秋季大会, 2014年09月, 日本語, 化学工学会, 福岡, 国内会議

    ポスター発表

  • Production of antibody fragments by transient gene expression in insect cells

    YAMAJI Hideki, KATSUDA Tomohisa, HAMADA Hirotsugu, MORI Keita

    16th European Congress on Biotechnology (ECB16), 2014年07月, 英語, European Federation of Biotechnology, Edinburgh, UK, 国際会議

    ポスター発表

  • 昆虫細胞を用いた一過性発現による機能性抗体タンパク質の生産

    森 慶太, 濵本 浩平, 宮口 尚子, 小川 隆文, 勝田 知尚, 山地 秀樹

    化学工学会第45回秋季大会, 2013年09月, 日本語, 化学工学会, 岡山市, 国内会議

    ポスター発表

  • Haematococcus pluvialisの増殖とアスタキサンチン生産に及ぼす培養温度の影響

    山田 浩之, 和田 壮太郎, GIANNELLI Luca, 山地 秀樹, 勝田 知尚

    化学工学会第45回秋季大会, 2013年09月, 日本語, 化学工学会, 岡山市, 国内会議

    ポスター発表

  • Photobioreactor design using CFD: mixing and light effects on microalgal cultures

    GIANNELLI Luca, WADA Sotaro, YAMADA Hiroyuki, YAMAJI Hideki, KATSUDA Tomohisa

    化学工学会第78年会, 2013年03月, 英語, 化学工学会, 豊中市, 国内会議

    口頭発表(一般)

  • Production of recombinant antibody molecules in insect cells

    MIYAGUCHI Naoko, HAMAMOTO Kouhei, OGAWA Takafumi, SONODA Hiroyuki, KATSUDA Tomohisa, YAMAJI Hideki

    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), 2012年11月, 英語, 日本動物細胞工学会, 名古屋市, 国際会議

    ポスター発表

  • Production and purification of Japanese encephalitis virus-like particles from recombinant insect cells

    HOTTA Yuri, MINAMITANI Azusa, NARITA Kazuma, KATSUDA Tomohisa, YAMAJI Hideki

    The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), 2012年11月, 英語, 日本動物細胞工学会, 名古屋市, 国際会議

    ポスター発表

  • 組換え昆虫細胞によるscFv-Fc融合タンパク質の生産

    薗田 啓之, 熊田 陽一, 勝田 知尚, 山地 秀樹

    第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • Computational fluid dynamics for enhancing the light distribution in a photobioreactor grown culture of Hematococcus pluvialis

    GIANNELLI Luca, WADA Sotaro, YAMAJI Hideki, KATSUDA Tomohisa

    第64回日本生物工学会大会, 2012年10月, 英語, 日本生物工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 大腸菌を用いた一本鎖抗体生産における終止コドンの影響

    山條 佑樹, 福丸 由夏, 山地 秀樹, 勝田 知尚

    化学工学会第44回秋季大会, 2012年09月, 日本語, 化学工学会, 仙台市, 国内会議

    ポスター発表

  • 気液界面を利用した高収率リポソーム生成法の開発

    相原 宏彦, 鈴木 洋, 勝田 知尚, 菰田 悦之, 日出間 るり

    化学工学会第44回秋季大会仙台, 2012年09月, 日本語, 仙台, 国内会議

    口頭発表(一般)

  • 昆虫細胞-バキュロウイルス系によるウイルス様粒子の生産

    山地 秀樹, 瀬川 麻衣子, 中村 匡崇, 勝田 知尚

    化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 炭化水素資化酵母Yarrowia lipolyticaによる油汚染土壌の修復のための無機栄養源の開発

    藤本 翔, 田沼 直樹, 勝田 知尚, 山地 秀樹

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • 昆虫細胞を用いた抗体タンパク質の分泌生産

    濵本 浩平, 宮口 尚子, 澤本 詩織, 小川 隆文, 勝田 知尚, 山地 秀樹

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • 昆虫細胞によるウイルス様粒子生産に及ぼす培養条件の検討

    成田 和馬, 堀田 有里, 南谷 梓, 永菅 尚, 勝田 知尚, 山地 秀樹

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • シャイン・ダルガーノ配列を改変した大腸菌による一本鎖抗体の流加培養生産

    福丸 由夏, 野上 達弘, 木原 麻那, 勝田 知尚, 山地 秀樹

    化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋, 国内会議

    ポスター発表

  • Efficient production of extracellular subviral particles of Japanese encephalitis virus by recombinant insect cells

    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

    IUMS 2011 Sapporo Congress, 2011年09月, 英語, International Union of Microbiological Societies, 札幌, 国内会議

    ポスター発表

  • Secretory production of virus-like particles by recombinant insect cells

    YAMAJI Hideki, NAGASUGA Takashi, TAKAHASHI Yusuke, NAKAMURA Masataka, KATSUDA Tomohisa, KUWAHARA Miwa, KONISHI Eiji

    ESACT 2011 Meeting, 2011年05月, 英語, European Society for Animal Cell Technology, Vienna, Austria, 国際会議

    ポスター発表

  • 炭化水素資化酵母Yarrowia lipolyticaを用いた油汚染土壌の修復における栄養源の添加条件の検討

    藤本 翔, 田沼 直樹, 勝田 知尚, 山地 秀樹

    第13回化学工学会学生発表会(神戸大会), 2011年03月, 日本語, 化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 大腸菌を用いた一本鎖抗体生産におけるシャイン・ダルガーノ配列の影響

    福丸 由夏, 野上 達弘, 勝田 知尚, 山地 秀樹

    第13回化学工学会学生発表会(神戸大会), 2011年03月, 日本語, 化学工学会, 神戸市, 国内会議

    口頭発表(一般)

  • 大腸菌におけるインクルージョンボディ生成に及ぼすシャイン・ダルガーノ配列の影響

    勝田 知尚, 福丸 由夏, 木原 麻那, 野上 達弘, 山地 秀樹

    化学工学会第76年会, 2011年03月, 日本語, 化学工学会, 東京都, 国内会議

    口頭発表(一般)

  • リポソームの応用事例

    勝田 知尚

    近畿バイオインダストリー振興会議第5回フォローアップ勉強会, 2011年01月, 日本語, 近畿バイオインダストリー振興会議, 大阪市, 国内会議

    口頭発表(招待・特別)

  • Optimized Shine-Dalgarno sequence for efficient production of single-chain Fv antibody in Escherichia coli

    NOGAMI Tatsuhiro, KIHARA Mana, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

    The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), 2010年12月, 英語, Canadian Society for Chemistry, American Chemical Society, Chemical Society of Japan, New Zealand Institute of Chemistry, Royal Australian Chemical Institute, Korean Chemical Society, Chinese Chemical Society, Honolulu, USA, 国際会議

    ポスター発表

  • Rapid production of functional antibody molecules by transient gene expression in insect cells

    SAWAMOTO Shiori, HAMAMOTO Kouhei, KATSUDA Tomohisa, YAMAJI Hideki

    Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, 2010年11月, 英語, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, 国際会議

    ポスター発表

  • Effect of signal peptide on the production of single-chain fv antibody by Escherichia coli

    NOGAMI Tatsuhiro, FUKUMARU Yuka, KATSUDA Tomohisa, YAMAJI Hideki

    Young Asian Biochemical Engineers Community (YABEC) Symposium 2010, 2010年11月, 英語, Young Asian Biochemical Engineers Community (YABEC), Taipei, Taiwan, 国際会議

    ポスター発表

  • Production of Japanese encephalitis virus-like particles in insect cell expression systems

    山地 秀樹, 永菅 尚, 高橋 裕輔, 中村 匡崇, 勝田 知尚, 桑原 三和, 小西 英二

    The 13th Asia Pacific Confederation of Chemical Engineering Congress (APCChE 2010), 2010年10月, 英語, Asia Pacific Confederation of Chemical Engineering, Taipei, Taiwan, 国際会議

    ポスター発表

  • プロテインAアフィニティクロマトグラフィーのための溶離条件検討法の開発

    加瀬 裕貴, 西山 拓也, 勝田 知尚, 山地 秀樹

    化学工学会第42回秋季大会, 2010年09月, 日本語, 化学工学会, 京都市, 国内会議

    ポスター発表

  • シャペロン共発現による大腸菌での一本鎖抗体 (scFv) 融合タンパク質の生産

    薗田 啓之, 熊田 陽一, 勝田 知尚, 山地 秀樹

    化学工学会第42回秋季大会, 2010年09月, 日本語, 化学工学会, 京都市, 国内会議

    ポスター発表

  • アルカン資化酵母Yarrowia lipolyticaを用いた土壌汚染油の分解

    田沼 直樹, 藤本 翔, 塩見 尚史, 勝田 知尚, 山地 秀樹

    化学工学会第42回秋季大会, 2010年09月, 日本語, 化学工学会, 京都市, 国内会議

    ポスター発表

  • Production of Japanese encephalitis virus-like particles using insect cells

    永菅 尚, 高橋 裕輔, 中村 匡崇, 勝田 知尚, 山地 秀樹

    The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2010), 2010年09月, 英語, The Japanese Association for Animal Cell Technology, 札幌市, 国際会議

    ポスター発表

  • Pichia pastorisを用いた一本鎖抗体生産における誘導初期のメタノール濃度の影響

    藤田 啓介, 秋山 直毅, 勝田 知尚, 山地 秀樹

    化学工学会第75年会, 2010年03月, 日本語, 化学工学会, 鹿児島, 国内会議

    口頭発表(一般)

  • 昆虫細胞-バキュロウイルス系を用いた抗体タンパク質の高効率生産

    澤本 詩織, 古田 貴憲, 勝田 知尚, 山地 秀樹

    第8回最先端バイオテクノロジー発表会, 2010年02月, 日本語, 化学工学会, 大阪, 国内会議

    ポスター発表

  • 一本鎖抗体の可溶性発現に及ぼすSD配列改変の影響

    野上 達弘, 木原 麻那, 佐野 宰久, 勝田 知尚, 山地 秀樹

    第8回最先端バイオテクノロジー発表会, 2010年02月, 日本語, 化学工学会, 大阪, 国内会議

    ポスター発表

  • Effects of Shine-Dalgarno sequence on the production of single-chain Fv antibody by Escherichia coli

    KIHARA Mana, NOGAMI Tatsuhiro, SANO Tadahisa, KATSUDA Tomohisa, YAMAJI Hideki

    Young Asian Biochemical Engineers Community (YABEC) Symposium 2009, 2009年12月, 英語, Young Asian Biochemical Engineers Community, Xiamen, 国際会議

    ポスター発表

  • Production of Japanese encephalitis virus-like particles by recombinant insect cells

    山地 秀樹, 高橋 裕輔, 中村 匡崇, 勝田 知尚, 桑原 三和, 小西 英二

    Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC '09), 2009年11月, 英語, APBioChEC '09 Committee, 神戸市, 国際会議

    口頭発表(一般)

  • Oil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolytica

    FUKUI Mayu, SHIOMI Shinji, TANUMA Naoki, KATSUDA Tomohisa, SHIOMI Naofumi, YAMAJI Hideki

    The 9th Asia-Pacific Biochemical Engineering Conference 2009, 2009年11月, 英語, The 9th Asia-Pacific Biochemical Engineering Conference 2009, Kobe, 国際会議

    ポスター発表

  • 大腸菌を用いた一本鎖抗体生産におけるSD配列の影響

    野上 達弘, 木原 麻那, 佐野 宰久, 勝田 知尚, 山地 秀樹

    化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 東広島市, 国内会議

    ポスター発表

  • 昆虫細胞を用いた日本脳炎ウイルス様粒子の生産

    高橋 裕輔, 永菅 尚, 中村 匡崇, 勝田 知尚, 山地 秀樹

    第61回日本生物工学会大会, 2009年09月, 日本語, 日本生物工学会, 名古屋市, 国内会議

    口頭発表(一般)

  • 昆虫細胞-バキュロウイルス系による抗体タンパク質の生産

    澤本 詩織, 鈴木 輔, 古田 貴憲, 小川 隆文, 勝田 知尚, 山地 秀樹

    化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 東広島市, 国内会議

    ポスター発表

  • ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用

    岩崎 大記, 千賀 至, 山下 貴史, 東内 雅博, 荻野 千秋, 清水 宣明, 勝田 知尚, 山地 秀樹

    化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 東広島市, 国内会議

    ポスター発表

  • 昆虫細胞を用いた日本脳炎ウイルスタンパク質の生産

    高橋 裕輔, 中村 匡崇, 古田 貴憲, 勝田 知尚, 山地 秀樹

    化学工学会第74年会, 2009年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • 一塩基変異を検出する昇温溶出アフィニティクロマトグラフィーに対するPCRの適用性

    勝田 知尚, 今西 誠, 加藤 滋雄, 山地 秀樹

    化学工学会第74年会, 2009年03月, 日本語, 化学工学会, 横浜市, 国内会議

    口頭発表(一般)

  • マイクロ流路内におけるリポソーム生成機構

    藤原 淳, 濱村 隼矢, 鈴木 洋, 勝田 知尚, 菰田 悦之, 薄井 洋基

    化学工学会第74年会, 2009年03月, 化学工学会, 横浜, 国内会議

    口頭発表(一般)

  • Production of adenovirus vector by 293 cells immobilized within porous biomass support particles

    MORISHITA Naoya, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki

    The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, 2008年11月, 英語, 日本動物細胞工学会, 福岡市, 国際会議

    ポスター発表

  • Low multiplicity infection of 293 cells for adenovirus vector production

    MORISHITA Naoya, YAMADA Kentaro, KUBO Shuji, GOTOH Akinobu, KATSUDA Tomohisa, FUKUDA Hideki, YAMAJI Hideki

    The 21th Annual and International Meeting of the Japanese Association for Animal Cell Technology, 2008年11月, 英語, 日本動物細胞工学会, 福岡市, 国際会議

    ポスター発表

  • High efficiency expression of soluble scFv in E. coli by use of linkers containing rare codons

    KAJIHARA Hideyuki, SAKAN Yoshinobu, KIHARA Mana, KIKUCHI Yasufumi, KUMADA Yoichi, KATSUDA Tomohisa, YAMAJI Hideki, KATOH Shigeo

    The 14th Symposium of Young Asian Biochemical Engineers’ Community, 2008年11月, 英語, Young Asian Biochemical Engineers’ Community, 東京都, 国際会議

    ポスター発表

  • Effects of the parameters of flashing light from blue emitting diodes on astaxanthin production by H. pluvialis

    SENGA Itaru, SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo

    Young Asian Biochemical Engineers Community (YABEC) Symposium 2008, 2008年11月, 英語, Young Asian Biochemical Engineers Community, Tokyo, 国際会議

    ポスター発表

  • A biochemical engineering study of photo-bioproces

    KATSUDA Tomohisa

    The 2 nd SCEJ (Kansai-Branch)/SSCCI Joint International Conference on Chemical Engineering, 2008年11月, 英語, SCEJ (Kansai-Branch) and SSCCI, Shanghai, China, 国際会議

    口頭発表(招待・特別)

  • Secretory production of Japanese encephalitis virus E protein by recombinant insect cells

    TAKAHASHI Yusuke, NAKAMURA Masataka, FURUTA Takanori, KATSUDA Tomohisa, YAMAJI Hideki

    “Bioseparation for Biorecognition and Bionanotechnology” Conference, 2008年10月, 英語, Hanyang University, Ansan, Korea, 国内会議

    ポスター発表

  • Detection of single nucleotide variation in amplified gene fragments using oligonucleotide-coupled affinity column

    KATSUDA Tomohisa, IMANISHI Makoto, YAMAJI Hideki, KATOH Shigeo

    “Bioseparation for Biorecognition and Bionanotechnology” Conference, 2008年10月, 英語, Hanyang University, Ansan, Korea, 国内会議

    ポスター発表

  • 多孔性粒子に固定化した大腸菌を用いる有用物質生産

    金 娜, 勝田 知尚, 福田 秀樹, 山地 秀樹

    化学工学会第40回秋季大会, 2008年09月, 日本語, 化学工学会, 仙台市, 国内会議

    ポスター発表

  • レアコドンリンカーによる大腸菌での可溶性scFvの高効率生産

    木原 麻那, 佐官 義信, 梶原 英之, 赤坂 翔, 勝田 知尚, 加藤 滋雄, 山地 秀樹

    化学工学会第40回秋季大会, 2008年09月, 日本語, 化学工学会, 仙台市, 国内会議

    ポスター発表

  • Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen species

    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Daiki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

    American Society for Microbiology 108th General Meeting, 2008年06月, 英語, American Society for Microbiology, Boston, USA, 国際会議

    ポスター発表

  • マイクロ流路を用いたリポソームの生成機構

    鈴木 洋, 浜村 隼矢, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基

    化学工学会 第73年会, 2008年03月, 日本語, 静岡, 国内会議

    口頭発表(一般)

  • Enhancement of growth and astaxanthin production of Haematococcus pluvialis by using airlift photobioreactor

    Ranjbar Reza, Inoue Ryota, Iwasaki Daiki, Katsuda Tomohisa, Katoh Shigeo

    化学工学会第73年会, 2008年03月, 英語, 化学工学会, 浜松, 国内会議

    口頭発表(一般)

  • ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化

    千賀 至, 山神 啓輔, 勝田 知尚, 萩野 千秋, 清水 宣明, 加藤 滋雄

    第6回 最先端バイオテクノロジー公開セミナー, 2008年02月, 日本語, 化学工学会関西支部、化学工学会バイオ部会, 西宮, 国内会議

    口頭発表(一般)

  • シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価

    武田 尚己, 勝田 知尚, 加藤 滋雄, 宮原 浩嘉, 井上 眞樹, 中村 周二

    第6回 最先端バイオテクノロジー公開セミナー, 2008年02月, 日本語, 化学工学会関西支部、化学工学会バイオ部会, 西宮, 国内会議

    口頭発表(一般)

  • High efficiency production of astaxantin by autotrophic cultivation of Haematococcua pluvialis in column photobioreactors

    RANJBAR Reza, INOUE Ryota, KATSUDA Tomohisa, KATOH Shigeo

    第6回 最先端バイオテクノロジー公開セミナー, 2008年02月, 英語, 化学工学会関西支部、化学工学会バイオ部会, 西宮, 国内会議

    口頭発表(一般)

  • Liposome Formation Characteristics with a Micro-Reactor

    Jun-ya HAMAMURA, Hiroshi SUZUKI, Tomohisa KATSUDA, Yoshiyuki KOMODA, Shigeo KATO, Hiromoto USUI

    11th Liposome Research Days Conference, 2008年, 英語, Yokohama, Japan, 国際会議

    口頭発表(一般)

  • Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination

    SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo

    上海市化学化工学会・化学工学会関西支部 第1回若手研究交流会, 2007年12月, 英語, 上海市化学化工学会、化学工学会関西支部, 大阪, 国際会議

    ポスター発表

  • 青色点滅光照射下でのH. pluvialisの培養に及ぼす光強度および周波数の影響

    白石 浩憲, 石津 直樹, 千賀 至, RANJBAR Reza, 勝田 知尚, 加藤 滋雄

    化学工学会山口大会, 2007年11月, 日本語, 化学工学会中国四国支部, 宇部, 国内会議

    ポスター発表

  • Application of PCR to Affinity Chromatography for SNP Detection

    KATSUDA Tomohisa, KATOH Shigeo

    The 6th Asia-Europe Biorecognition Engineering Society Conference, 2007年11月, 英語, Asia-Europe Biorecognition Engineering Society, Taoyuan, Taiwan, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • Oxidative treatment of H. pluvialis with the sonocatalytic reaction

    KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Hiroki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

    YABEC2007, 2007年10月, 英語, Young Asian Biochemical Engineers Community, Seoul, Korea, 国際会議

    ポスター発表

  • Effects of the intensity and frequency on H. pluvialis grown under flashing light illumination

    SHIRAISHI Hironori, ISHIZU Naoki, KATSUDA Tomohisa, KATOH Shigeo

    YABEC2007, 2007年10月, 英語, Young Asian Biochemical Engineers Community, Seoul, Korea, 国際会議

    ポスター発表

  • A Biochemical Engineering Study of Bioproductions using Photobioreactor

    KATSUDA Tomohisa

    2007 Fall KSBB Meeting & International Symposium, 2007年10月, 英語, The Korean Society for Biotechnology and Bioengineering, Daegu, Korea, 国際会議

    [招待有り]

    口頭発表(招待・特別)

  • 二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導

    勝田 知尚, 荻野 千秋, 山神 啓輔, 加藤 滋雄, 清水 宣明

    第59回生物工学会大会, 2007年09月, 日本語, 日本生物工学会, 広島, 国内会議

    口頭発表(一般)

  • 気泡塔型フォトバイオリアクターを用いたヘマトコッカス藻による効率的アスタキサンチン生産

    井上 量太, Ranjbar Reza, 勝田 知尚, 加藤 滋雄

    化学工学会第39回秋季大会, 2007年09月, 日本語, 化学工学会, 札幌, 国内会議

    ポスター発表

  • マイクロキャピラリー内におけるリポソーム生成特性に関する研究

    濱村 隼矢, 鈴木 洋, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基

    化学工学会 第39回秋季大会, 2007年09月, 日本語, 北海道大学, 国内会議

    口頭発表(一般)

  • フォトバイオリアクターによる 有用物質生産のための生物化学工学的検討

    勝田 知尚

    第59回生物工学会大会, 2007年09月, 日本語, 日本生物工学会, 広島, 国内会議

    [招待有り]

    その他

  • Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasounds

    KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

    13th European Congress on Biotechnology, 2007年09月, 英語, European Federation of Biotechnology, Barcelona, Spain, 国際会議

    ポスター発表

  • DNAアフィニティカラムを用いるSNP検出法へのPCR増幅法の応用

    今西 誠, 西和田 靖, 福井 真由, 勝田 知尚, 加藤 滋雄

    化学工学会第39回秋季大会, 2007年09月, 日本語, 化学工学会, 札幌, 国内会議

    ポスター発表

  • 青色点滅光照射下のH. pluvialisにおけるアスタキサンチン生産量と光強度との関係

    白石 浩憲, 山神 啓輔, RANJBAR Reza, 千賀 至, 勝田 知尚, 加藤 滋雄

    化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議

    口頭発表(一般)

  • メタノール資化性酵母を用いた一本鎖抗体生産におけるメタノール供給方法

    大西 由夏, 山脇 伸哉, 松本 偉大, 塩見 尚史, 勝田 知尚, 加藤 滋雄

    化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議

    口頭発表(一般)

  • シリカ系プロテインAアフィニティクロマトグラフィー担体の性能評価

    武田 尚己, 今田 優美, 勝田 知尚, 加藤 滋雄, 宮原 浩嘉, 井上 眞樹, 中村 周二

    化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議

    口頭発表(一般)

  • Optimization of silica based media for antibody purification by protein A affinity chromatography

    IMADA Masami, TAKEDA Naoki, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji, KATOH Shigeo

    YABEC 2006, 2006年11月, 英語, Young Asian Biochemical Engineers Community, Kaohsiung, 国際会議

    ポスター発表

  • Effects of flashing light from blue LEDs on the cell growth and astaxanthin production of Haematococcus pluvialis

    YAMAGAMI Keisuke, SHIRAISHI Hironori, RANJBARDAVIJANI Reza, SHIMAHARA Kazumichi, KATSUDA Tomohisa, KATOH Shigeo

    YABEC 2006, 2006年11月, 英語, Young Asian Biochemical Engineers Community, Kaohsiung, 国際会議

    ポスター発表

  • Optimization of silica-based matrices for antibody purification by protein A affinity chromatography

    KATOH Shigeo, IMADA Masami, KATSUDA Tomohisa, INOUE Masaki, MIYAHARA Hiroyoshi, NAKAMURA Shuji

    26th international symposium on the separation of proteins, peptides and polynucleotides, 2006年10月, 英語, ISPPP, Innsbruck, 国際会議

    口頭発表(一般)

  • 昇温溶出アフィニティークロマトグラフィーによる一塩基多型検出法の開発

    勝田 知尚

    化学工学会バイオ部会員フォーマルセミナー, 2006年09月, 日本語, 化学工学会バイオ部会, 福岡, 国内会議

    口頭発表(一般)

  • メタノール資化性酵母Pichia pastorisの連続培養による一本鎖抗体生産

    山脇 伸哉, 松本 偉大, 大西 由夏, 勝田 知尚, 塩見 尚史, 加藤 滋雄

    第58回生物工学会大会, 2006年09月, 日本語, 生物工学会, 大阪, 国内会議

    口頭発表(一般)

  • ヘマトコッカスによるアスタキサンチン生産における青色点滅光照射の影響

    勝田 知尚, 白石 浩憲, 山神 啓輔, 千賀 至, 嶋原 一道, 加藤 滋雄

    第58回生物工学会大会, 2006年09月, 日本語, 生物工学会, 大阪, 国内会議

    口頭発表(一般)

  • ヘマトコッカスによるアスタキサンチン生産における青色パルス光照射条件の検討

    白石 浩憲, 山神 啓輔, RANJBAR Reza, 勝田 知尚, 加藤 滋雄

    化学工学会第38回秋季大会, 2006年09月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • Pseudomonas sp. を用いる自己固定化バイオレメディエーションによるシアヌル酸分解

    塩見 尚史, 有井 健治, 中井 裕章NAKAI Hiroaki, 山口 豊, 勝田 知尚, 加藤 滋雄

    化学工学会第38回秋季大会, 2006年09月, 日本語, 化学工学会, 福岡, 国内会議

    口頭発表(一般)

  • 多層式光培養槽を用いた緑藻Haematococcus pluvialisの光培養生産

    吉村 誠司, 井上 量太, RANJBER Reza, 勝田 知尚, 加藤 滋雄

    化学工学会第71年会, 2006年03月, 英語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 昇温溶出アフィニティークロマトグラフィーを用いるSNP検出法の開発と検討

    西和田 靖, 嘉村 光真, 勝田 知尚, 加藤 滋雄

    化学工学会第71年会, 2006年03月, 英語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • Haematocuccus pluvialisによる効率的アスタキサンチン生産のためのパルス光照射

    嶋原 一道, 勝田 知尚, 山神 啓輔, 白石 浩憲, 加藤 滋雄

    化学工学会第71年会, 2006年03月, 英語, 化学工学会, 東京, 国内会議

    口頭発表(一般)

  • 半連続培養における光合成細菌の増殖安定性におよぼす鉄イオン濃度の影響

    勝田 知尚, YEGANI Reza, 吉村 誠司, 森谷 一徳, 井上 量太, 加藤 滋雄

    関西地区3学協会合同大会, 2005年11月, 日本語, 近畿化学協会, 化学工学会, 触媒学会, 大阪, 国内会議

    口頭発表(一般)

  • 酸性雨により酸性化された池や土壌のバイオレメディエーション

    塩見 尚史, 鍋島 理咲子, 安田 多賀子, 岩崎 沙織, 山口 豊, 中井 裕章, 勝田 知尚, 加藤 滋雄

    関西地区3学協会合同大会, 2005年11月, 日本語, 近畿化学協会, 化学工学会, 触媒学会, 大阪, 国内会議

    口頭発表(一般)

  • 光合成細菌の半連続培養における操作条件の検討

    吉村 誠司, YEGANI Reza, 森谷 一徳, 井上 量太, 勝田 知尚, 加藤 滋雄

    関西地区3学協会合同大会, 2005年11月, 日本語, 近畿化学協会, 化学工学会, 触媒学会, 大阪, 国内会議

    ポスター発表

  • 固定化DNAアフィニティークロマトグラフィーを用いるSNP検出

    西和田 靖, 嘉村 光真, 勝田 知尚, 加藤 滋雄

    関西地区3学協会合同大会, 2005年11月, 日本語, 近畿化学協会, 化学工学会, 触媒学会, 大阪, 国内会議

    ポスター発表

  • 凝集性微生物を用いた農薬汚染土壌の浄化法の検討

    中井 裕章, 山口 豊, 安田 多賀子, 有井 建治, 坂井 豊英, 勝田 知尚, 加藤 滋雄, 塩見 尚史

    平成17年度日本生物工学会大会, 2005年11月, 日本語, 日本生物工学会, 筑波, 国内会議

    口頭発表(一般)

  • Rapid SNP Detection by Temperature Gradient Affinity Chromatography

    KATOH Shigeo, KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi

    International Symposium on the Separation of Proteins, Peptides & Polynucleotides 2005, 2005年11月, 英語, International Symposium on the Separation of Proteins, Peptides & Polynucleotides, St. Pete Beach, Florida, 国際会議

    口頭発表(一般)

  • LEDを用いた緑藻ヘマトコッカスによる効率的アスタキサンチン生産

    嶋原 一道, 山神 啓輔, 白石 浩憲, 勝田 知尚, 加藤 滋雄

    関西地区3学協会合同大会, 2005年11月, 日本語, 近畿化学協会, 化学工学会, 触媒学会, 大阪, 国内会議

    ポスター発表

  • Detection of SNP Using Affinity Chromatography of Immobilized Oligonucleotide

    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo

    YABEC 2005, 2005年10月, 英語, Young Asia Biochemical Engineers Community, Beijing, China, 国際会議

    口頭発表(一般)

  • 光合成細菌の半連続培養における操作条件の検討

    勝田 知尚, YEGANI Reza, 吉村 誠司, 森谷 一徳, 井上 量太, 加藤 滋雄

    化学工学会第37回秋季大会, 2005年09月, 日本語, 化学工学会, 岡山, 国内会議

    ポスター発表

  • Growth Sstability of Photosynthetic Bacteria : Effects of Supplemental Illumination and Ferrous Ion Concentration

    YEGANI Reza, YOSHIMURA Satoshi, KATSUDA Tomohisa, KATOH Shigeo

    The 12th European Congress on Biotechnology, 2005年08月, 英語, European Federation of Biotechnology, Copenhagen, Denmark, 国際会議

    ポスター発表

  • Fed-Batch Cultivation of Haematococcus pluvialis under Illumination with LEDs for Production of Astaxanthin

    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo

    The 12th European Congress on Biotechnology, 2005年08月, 英語, European Federation of Biotechnology, Copenhagen, Denmark, 国際会議

    ポスター発表

  • Temperature Gradient Elution of DNAs from an Oligo-DNA Immobilized Column

    KATSUDA Tomohisa, KAMURA Mitsumasa, NISHIJIMA Ken, NISHIWADA Yasushi, KATOH Shigeo

    分離技術会年会2005, 2005年06月, 英語, 分離技術会, 大阪, 国内会議

    ポスター発表

  • Effects of Feeding Method on Astaxanthin Production by Haematococcus pluvialis

    KATSUDA Tomohisa, LABABPOUR Abdolmajid, SHIMAHARA Kazumichi, KATOH Shigeo

    Asia-Pacific Biochemical Engineering Conference 2005, 2005年05月, 英語, The Korean Society for Biotechnology and Bioengineering, The Korean Institute of Chemical Engineers, Jeju, Korea, 国際会議

    ポスター発表

  • Detection of SNP using Affinity Chromatography of Immobilized Oligonucleotide

    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, NISHIWADA Yasushi, KATOH Shigeo

    The 4th Asia-Europe Biorecognition Engineering Society Conference, 2005年05月, 英語, Asia-Europe Biorecognition Engineering Society, Ansan, Korea, 国際会議

    口頭発表(一般)

  • 緑藻ヘマトコッカスによるアスタキサンチン生産における栄養源供給法の検討

    勝田 知尚, LABABPOUR Abdolmajid, 京井 祥哲, 嶋原 一道, 加藤 滋雄

    化学工学会第70年会, 2005年, 日本語, 化学工学会, 名古屋, 国内会議

    口頭発表(一般)

  • オリゴDNA固定化カラムにおけるDNAの昇温溶出挙動の検討

    嘉村 光真, 西島 研, 西和田 靖, 勝田 知尚, 加藤 滋雄

    化学工学会第70年会, 2005年, 日本語, 化学工学会, 名古屋, 国内会議

    口頭発表(一般)

  • 1本鎖抗体のペプチドリンカーが抗原結合に及ぼす影響

    熊田 陽一, 川崎 朋美, 加藤 滋雄

    日本生物工学会平成16年度大会, 2004年09月, 日本語, 日本生物工学会, 名城大学, 国内会議

    口頭発表(一般)

  • フォトバイオリアクターにおける光強度分布と増殖速度安定性の工学的検討

    イエガニ レザ, 吉村 誠司, 森谷 一徳, 勝田 知尚, 加藤 滋雄

    日本生物工学会平成16年度大会, 2004年, 日本語, 日本生物工学会, 名城大学, 国内会議

    口頭発表(一般)

  • オリゴDNA固定化担体を用いた一塩基多型(SNP)の検出法の検討

    西島 研, 嘉村 光真, 勝田 知尚, 加藤 滋雄

    日本生物工学会平成16年度大会, 2004年, 日本語, 日本生物工学会, 名城大学, 国内会議

    口頭発表(一般)

  • Instability of Growth Rate in Dense Culture of Photosynthetic Cells

    YEGANI Reza, YOSHIMURA Satoshi, MORIYA Kazunori, KATSUDA Tomohisa, KATOH Shigeo

    YABEC'04 Symposium, 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Induction of Astaxanthin Accumulation in H.Pluvialis by Illumination with LED Lamps and Fed Batch Addition of Nutrients

    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo

    YABEC'04 Symposium, 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Growth and Astaxanthin Accumulation in H.pluvialis Cultures Illuminated by LED Lamps

    Lababpour Abdolmajid, 羽田 圭司, 島原 一道, 勝田 知尚, 加藤 滋雄

    化学工学会第69年会, 2004年, 英語, 化学工学会, 大阪府立大学, 国内会議

    口頭発表(一般)

  • Development of Oligonucleotide Immobilized Capillary Chromatography

    KATSUDA Tomohisa, NISHIJIMA Ken, KAMURA Mitsumasa, HOSSAIN Zakir, KATOH Shigeo

    The 2nd International Conference on Bioseparation Engineering, 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • Astaxanthin Production by H. Pluvialis in Sequential Batch Followed by Fed-Batch Culture Illuminated by LED Lamps

    LABABPOUR Abdolmajid, KATSUDA Tomohisa, KATOH Shigeo

    2004 AIChE Annual Meeting, 2004年, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産におよぼす青色パルス光の影響

    嶋原 一道, LABABPOUR Abdolmajid, 羽田 圭志, 勝田 知尚, 加藤 滋雄

    第55回日本生物工学会大会, 2003年09月, 日本語, 日本生物工学会, 熊本大学, 国内会議

    口頭発表(一般)

  • 光合成微生物の半連続培養における増殖安定性におよぼす光強度分布の影響

    勝田 知尚, イエガニ レザ, 吉村 誠司, 藤井 信彰, 五十嵐 啓介, 加藤 滋雄

    第55回日本生物工学会大会, 2003年09月, 日本語, 日本生物工学会, 熊本大学, 国内会議

    口頭発表(一般)

  • パルス光照射下におけるヘマトコッカスによるアスタキサンチン生産

    勝田 知尚, LABABPOUR Abdolmajid, 嶋原 一道, 羽田 圭志, 加藤 滋雄

    化学工学会 第36回秋季大会, 2003年09月, 日本語, 化学工学会, 東北大学, 国内会議

    口頭発表(一般)

  • The effects of light intensity distribution on the stability of growth rate of a photosynthetic microorganism Rhodobacter capsulatus

    YEGANI Reza, 藤井 信彰, 五十嵐 啓介, 吉村 誠司, 勝田 知尚, 加藤 滋雄

    化学工学会 第36回秋季大会, 2003年09月, 英語, 化学工学会, 東北大学, 国内会議

    口頭発表(一般)

  • Cultivation of microalga in photobioreactors illuminated with LEDs

    KATOH Shigeo, LABABPOUR Abdolmajid, KATSUDA Tomohisa

    The 6th Italian Conference on Chemical and Process Engineering, 2003年06月, 英語, 未記入, 未記入, 国際会議

    口頭発表(一般)

  • プロテインA担体における抗体の拡散・吸着挙動の検討

    林 航平, 勝田 知尚, 山地 秀樹

    化学工学会第87年会, 2022年03月, 英語

    ポスター発表

  • 異種タンパク質を提示するインフルエンザウイルス様粒子の昆虫細胞を用いた生産

    市川 裕大, 松田 拓也, Prihardi Kahar, 勝田 知尚, 山地 秀樹

    化学工学会第87年会, 2022年03月, 英語

    ポスター発表

  • 分離精製(膜・クロマト分離)

    勝田 知尚

    日本生物工学会100周年記念事業教育セミナー「培養技術勉強会」, 2021年11月, 日本語

    [招待有り]

    公開講演,セミナー,チュートリアル,講習,講義等

  • 遺伝子組換え昆虫細胞樹立へのゲノム編集技術の応用

    松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹

    化学工学会第52回秋季大会, 2021年09月

  • ゲノム編集技術を用いた遺伝子組換え昆虫細胞の樹立

    松田 拓也, 戸上 真也, 増見 恭子, Prihardi Kahar, 勝田 知尚, 山地 秀樹

    第34回日本動物細胞工学会2021年度大会 (JAACT2021), 2021年07月

  • プロテインA固定化マイクロアフィニティカラムを用いた疑似オンラインIgG濃度測定法の開発

    川村 和樹, 勝田 知尚, 山地 秀樹

    化学工学会第86年会, 2021年03月, 日本語

    ポスター発表

  • Production of influenza virus-like particles using recombinant insect cells

    Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

    The 33rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2020 Fuchu), 2020年11月

  • 昆虫細胞を用いたインフルエンザウイルス様粒子の生産

    松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹

    化学工学会第51回秋季大会, 2020年09月, 日本語

  • Adsorption of immunoglobulin G in protein A resins studied by confocal laser scanning microscopy taking account of the optical hindrance inside a particle

    Tomohisa Katsuda, Tomoki Yamaguchi, Naruaki Inoue, Yuki Kase, Kou Ota, Hideki Yamaj

    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019年09月, 英語

    ポスター発表

  • Production of influenza virus proteins in recombinant insect cells

    Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji

    18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019年09月, 英語

    ポスター発表

  • 微細藻の細胞分裂に及ぼす培養温度・光強度の周期的制御の影響

    勝田 知尚, 森本 高章, 桑井 仁葵, 山地 秀樹

    第71回日本生物工学会大会, 2019年09月, 日本語

    口頭発表(一般)

  • 組換え昆虫細胞によるインフルエンザウイルス抗原タンパク質の生産

    松田 拓也, 増見 恭子, 勝田 知尚, 山地 秀樹

    第32回日本動物細胞工学会2019年度大会 (JAACT2019), 2019年07月, 日本語

    ポスター発表

  • Production of influenza virus-like particles in insect cells

    Hideki Yamaji, Takuya Matsuda, Toshikazu Tanijima, Kyoko Masumi-Koizumi, Tomohisa Katsuda

    26th ESACT (the European Society for Animal Cell Technology) Meeting (ESACT 2019), 2019年05月, 英語

    ポスター発表

  • 微生物の光合成を利用した有用物質生産ーエネルギーと食品への活用ー

    勝田 知尚

    第108回微小光学研究会, 2008年07月, 日本語

    [招待有り]

    公開講演,セミナー,チュートリアル,講習,講義等

  • Development of a new SNP detectable method by use of the immobilized oligonucleotide capillary chromatography

    Tomohisa Katsuda, Ken Nishijima, Mitsumasa Kamura, Shigeo Katoh

    Asia-Europe Symposium on Biomolecular Recognition, 2003年05月, 英語

    口頭発表(一般)

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産における光照射法の検討

    勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄

    化学工学会第68年会, 2003年03月, 日本語

    口頭発表(一般)

  • 二槽式バイオリアクターによる光合成微生物の生産性の向上

    藤井 信彰, 五十嵐 啓介, Reza Yegani, 勝田 知尚, 加藤 滋雄

    化学工学会第68年会, 2003年03月, 日本語

    口頭発表(一般)

  • Astaxanthin production by Haematococcus pluvialis using LED lamps

    Tomohisa Katsuda, Abdolmajid Lababpour, Kazumichi Shimahara, Shigeo Katoh

    Young Asian Biochemical Engineering Community (YABEC) Symposium 2002, 2002年11月, 英語

    口頭発表(一般)

  • 緑藻Haematococcus pluvialisによるアスタキサンチン生産に及ぼす照射光の影響

    Abdolmajid Lababpour, 嶋原 一道, 勝田 知尚, 加藤 滋雄

    化学工学会第35回秋季大会, 2002年09月, 英語

    口頭発表(一般)

  • 発光ダイオードを用いた緑藻Haematococcus pluvialisによるアスタキサンチン生産

    勝田 知尚, Abdolmajid Lababpour, 嶋原 一道, 加藤 滋雄

    化学工学会第35回秋季大会, 2002年09月, 日本語

    口頭発表(一般)

  • タンパク質のリフォールディングにおよぼす変性剤除去の影響

    宇高 太郎, 神野 一也, M.D. Farshbaf, 勝田 知尚, 加藤 滋雄

    化学工学会第35回秋季大会, 2002年09月, 日本語

    口頭発表(一般)

  • 高効率土壌汚染浄化法を利用した酸性雨の中和

    安田 多賀子, 岩崎 沙織, 勝田 知尚, 塩見 尚史, 加藤 滋雄

    化学工学会鳥取大会, 2002年07月, 日本語

    口頭発表(一般)

  • 光合成微生物培養液中における光強度減衰モデルの適用性

    勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄

    化学工学会第67年会, 2002年03月, 日本語

    口頭発表(一般)

  • B. subtilisの自己固定化能力を利用した酸性雨の土壌中での中和

    安田 多賀子, 岩崎 沙織, 井上 洋子, 楠本 智子, 勝田 知尚, 塩見 尚史, 加藤 滋雄

    化学工学会第67年会, 2002年03月, 日本語

    口頭発表(一般)

  • Light attenuation in suspension of purple bacterium Rhodobacter capsulatus and green alga Chlamydomonas reinhardtii

    Tomohisa Katsuda, Nobuaki Fujii, Natsushi Takata, Shigeo Katoh

    Young Asian Biochemical Engineering Community (YABEC) Symposium 2001, 2001年10月, 英語

    口頭発表(一般)

  • 光合成微生物培養液中における光強度減衰

    勝田 知尚, 藤井 信彰, 高田 夏詩, 加藤 滋雄

    日本生物工学会光合成微生物研究部会夏期研究集会, 2001年08月, 日本語

    口頭発表(一般)

  • 光合成微生物培養液中での光強度減衰に及ぼす光源の影響

    勝田 知尚, 藤井 信彰, 加藤 滋雄

    化学工学会福井大会, 2001年07月, 日本語

    口頭発表(一般)

  • 光合成微生物培養液中での光強度減衰

    勝田 知尚, 藤井 信彰, 大嶋 寛, 加藤 滋雄

    化学工学会第66年会, 2001年04月, 日本語

    口頭発表(一般)

  • テーラー渦型反応器によるタンパク質の連続リフォールディング

    加藤 芳寛, M.D. Farshbaf, 宇高 太郎, 野田 秀夫, 勝田 知尚, 加藤 滋雄

    化学工学会第66年会, 2001年04月, 日本語

    口頭発表(一般)

  • 円筒型光リアクターの新規な光強度分布モデルと光合成細菌による水素生産への適用

    勝田 知尚, 有本 武司, 五十嵐 幸一, 東 雅之, 大嶋 寛

    化学工学会第32回秋季大会, 1999年09月, 日本語

    口頭発表(一般)

  • 円筒型フォトバイオリアクターの新規な光強度分布モデルと光合成微生物による水素生産への適用

    勝田 知尚, 大嶋 寛

    日本生物工学会光合成微生物研究部会夏期研究集会, 1999年08月, 日本語

    口頭発表(一般)

  • エタノールアミンを窒素源として用いた光合成細菌Rhodobacter capsulatusによる水素生産

    勝田 知尚, 大嶋 寛

    日本生物工学会光合成微生物研究部会夏期研究集会, 1998年08月, 日本語

    口頭発表(一般)

  • エタノールアミンを窒素源として用いた、Rhodobacter capsulatusによる水素生産

    勝田 知尚, 大嶋 寛, 東 雅之, 加藤 錠治, 高桑 進

    平成9年度日本生物工学会大会, 1997年09月, 日本語

    口頭発表(一般)

  • Hydrogen detectable color indicator for screening of microorganisms

    Tomohisa Katsuda, Hiroshi Ooshima, Masayuki Azuma, Jyoji Kato

    化学工学会第29回秋季大会, 1996年09月, 英語

    ポスター発表

所属学協会

  • 日本生物工学会

  • 化学工学会

共同研究・競争的資金等の研究課題

  • 勝田 知尚

    学術研究助成基金助成金/基盤研究(C), 2017年04月 - 2020年03月, 研究代表者

    競争的資金

  • 産業技術研究「活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発」

    勝田 知尚

    2008年, 研究代表者

    競争的資金

  • 鈴木 洋

    科学研究費補助金/基盤研究(C), 2008年

    競争的資金

  • 昇温溶出アフィニティークロマトグラフィーによる一塩基多型の検出

    勝田 知尚

    2007年, 研究代表者

    競争的資金

  • 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発

    勝田 知尚

    2006年, 研究代表者

    競争的資金

  • 活性酸素ストレスによる細胞生理の制御に基づいた機能性バイオプロダクト生産技術の開発

    勝田 知尚

    2005年, 研究代表者

    競争的資金

産業財産権

  • リポソームの製造方法

    鈴木 洋, 菰田 悦之, 勝田 知尚

    特願2012-553652, 2012年01月06日, 大学長, 特許5904555, 2016年03月25日

    特許権

  • リポソーム製剤の製造方法

    鈴木 洋, 勝田 知尚, 加藤 滋雄, 菰田 悦之, 薄井 洋基

    特願2007-134414, 2007年05月21日, 大学長, 特許5126874, 2012年11月09日

    特許権