Directory of Researchers

MANTANI Youhei
Graduate School of Agricultural Science / Department of Bioresource Science
Assistant Professor
Agriculture
Last Updated :2021/06/12

Researcher Profile and Settings

Affiliation

  • <Faculty / Graduate School / Others>

    Graduate School of Agricultural Science / Department of Bioresource Science
  • <Related Faculty / Graduate School / Others>

    Faculty of Agriculture / Department of Bioresource Science

Teaching

Research Activities

Research Areas

  • Life sciences / Zoological sciences

Published Papers

  • Tetsushi Hirano, Yuka Miyata, Shizuka Kubo, Shuji Ohno, Kanoko Onaru, Mizuki Maeda, Sayaka Kitauchi, Misaki Nishi, Yoshiaki Tabuchi, Yoshinori Ikenaka, Takahiro Ichise, Shouta M M Nakayama, Mayumi Ishizuka, Koji Arizono, Keisuke Takahashi, Keisuke Kato, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi

    Neonicotinoids, which act as agonists of the nicotinic acetylcholine receptors of insects, are widely used pesticides worldwide. Although epidemiological studies revealed that the detection amounts of neonicotinoids in urine are higher in the elderly population than other age-groups, there is no available information regarding the risks of neonicotinoids to older mammals. This study was aimed to investigate aging-related differences in the behavioral effects of the neonicotinoid pesticide clothianidin (CLO). We acutely administered a sub-NOAEL level (5 mg/kg) of CLO to adult (12-week-old) and aging (90-week-old) mice and conducted four behavioral tests focusing on the emotional behavior. In addition, we measured the concentrations of CLO and its metabolites in blood, brain and urine. There were age-related changes in most parameters in all behavioral tests, and CLO significantly decreased the locomotor activity in the open field test and elevated plus-maze test in the aging group, but not in the adult group. The concentrations of most CLO and its metabolites were significantly higher in the blood and brain and were slightly lower in the urine in the aging group compared to the adult group. These findings should contribute to our understanding of age-related differences in the adverse effects of neonicotinoids in mammals.

    15 May 2021, Toxicology letters, 342, 95 - 103, English, International magazine

    Scientific journal

  • Youhei Mantani, Tomohiro Haruta, Satoki Nakanishi, Nanami Sakata, Hideto Yuasa, Toshifumi Yokoyama, Nobuhiko Hoshi

    Several types of macrophages have been reported in the intestinal mucosa, but their histological localization remains ambiguous. Here, we obtained detailed information about ultrastructural and phenotypical diversity of macrophage-like cells (MLCs) in the rat ileal mucosa using immunofluorescent analysis and serial block-face scanning electron microscopy (SBF-SEM). The results revealed that the cells immunopositive for CD68, the pan-macrophage marker, included CD163-CD4+, CD163+CD4+, and CD163-CD4- cells in the lamina propria (LP) of the intestinal villus and around the crypt. CD68+CD4+CD163- cells seemed to be preferentially localized in the intestinal villus, whereas CD68+CD163+CD4+ cells were frequently localized around the crypt. SBF-SEM analysis identified three types of MLCs in the ileal mucosa, which were tentatively named types I-III MLC based on aspects of the 3D-ultrastructure, such as the localization, quantity of lysosomes, endoplasmic reticulum, and exoplasm. Type I and II MLCs were localized in the villous LP, while type III MLCs were localized around the crypt, although type II MLCs were a minor population. All three MLC types extended their cellular processes into the epithelium, with type I MLCs showing the greatest abundance of extended processes. Type I MLCs in the upper portion of the intestinal villus showed a higher level of attachment to intraepithelial lymphocytes (IELs) compared to type III MLCs around the crypt. These findings suggest that macrophages of the rat ileal mucosa differed by region along the longitudinal axis of the villous tip-crypt from the perspective of ultrastructure, cellular composition, localization, and interactions with IELs.

    07 May 2021, Cell and tissue research, English, International magazine

    Scientific journal

  • Sayaka Kitauchi, Mizuki Maeda, Tetsushi Hirano, Yoshinori Ikenaka, Misaki Nishi, Asuka Shoda, Midori Murata, Youhei Mantani, Toshifumi Yokoyama, Yoshiaki Tabuchi, Nobuhiko Hoshi

    Recently, developmental exposure to clothianidin (CLO) has been shown to cause reproductive toxicity in male mice, but the effects in female mice remain to be clarified. Pregnant C57BL/6N mice were given a no-observed-adverse-effect-level (NOAEL) dose of CLO until weaning. We then examined ovaries of 3- or 10-week-old female offspring. In the CLO-administered group, morphological changes, a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 (GPx4), and activation of genes in the steroid hormone biosynthesis pathway were observed in 3-week-old mice, and decreases of GPx4 immunoreactivity, 17OH-progesterone and corticosterone levels were observed in 10-week-old mice, along with high rates of infanticide and severe neglect, providing new evidence that developmental exposure to CLO affects juvenile and adult mice differently.

    24 Apr. 2021, The Journal of veterinary medical science, 83 (4), 746 - 753, English, Domestic magazine

    Scientific journal

  • Mizuki Maeda, Sayaka Kitauchi, Tetsushi Hirano, Yoshinori Ikenaka, Misaki Nishi, Asuka Shoda, Midori Murata, Youhei Mantani, Yoshiaki Tabuchi, Toshifumi Yokoyama, Nobuhiko Hoshi

    Recently, it has been reported that neonicotinoid pesticides (NNs) are transferred from mother to child and are assumed to affect the next generation, but the behavioral effects of NN exposure at different developmental stages have not been investigated. We exposed mice to no-observed-adverse-effect level (NOAEL) doses of clothianidin (CLO) during the fetal and lactational period, and then evaluated the neurobehavioral effects in juvenile and adult mice. Significant increases in anxiety-like behavior and locomotor activity were observed in juveniles and adults, respectively, and neuronal activity and neurogenesis in the hippocampal dentate gyrus were affected in both stages. These results suggest that fetal and lactational exposure to CLO may inhibit neurogenesis and cause different behavioral abnormalities at different developmental stages.

    03 Apr. 2021, The Journal of veterinary medical science, 83 (3), 542 - 548, English, Domestic magazine

    Scientific journal

  • Mizuki Maeda, Toshifumi Yokoyama, Sayaka Kitauchi, Tetsushi Hirano, Youhei Mantani, Yoshiaki Tabuchi, Nobuhiko Hoshi

    Fipronil (FPN) is a systemic insecticide that antagonizes the gamma-aminobutyric acid type A (GABAA) receptors in insects. Recently, adverse effects of FPN on mammals have been reported, but most of those were caused by high doses of FPN and additives in the products. We investigated the effects of low-dose pure FPN on the emotional behavior of mice. Nine-week-old male mice conducted behavioral tests 24 hr after FPN administration by gavage at doses of 0.05 or 5 mg/kg based on the no-observed-effect level (NOEL), showed a significant increase in locomotor activity and dose-dependent responses on the time they spent in the central zone in the open field test. Pure FPN below the NOEL dose may affect the emotional behavior of mice.

    05 Mar. 2021, The Journal of veterinary medical science, 83 (2), 344 - 348, English, Domestic magazine

    Scientific journal

  • Masaya Arai, Youhei Mantani, Satoki Nakanishi, Tomohiro Haruta, Miho Nishida, Hideto Yuasa, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Eosinophils are abundantly present in intestinal mucosa. However, the morphological characteristics of their cellular population are still largely unknown. In this study, we examine their characteristics in the rat ileal mucosa using histological and ultrastructural methods. The results indicated that ileal eosinophils could be distinguished into two main groups based on their nuclear shapes and distribution: eosinophils with spheric or reniform nuclei mainly localized in the villous region and eosinophils with annular or bacilliform nuclei as the major population around crypts. Immunohistochemical analysis revealed that all eosinophils in the lamina propria (LP) were immunopositive for CD11b, whereas eosinophils in LP of the intestinal villus but not those in LP around the crypt, were immunopositive for CD11c. Three-dimensional ultrastructural analysis using serial block-face scanning electron microscopy showed that the eosinophils with spheric or reniform nuclei were abundant in the upper portions of the intestinal villus, whereas those with annular nuclei were abundant in the lower portions of the intestinal villus and around crypts. The eosinophils with spheric or reniform nuclei possessed broader cellular bodies with greater abundance of surface projections compared with those with annular nuclei. Eosinophils in the upper portions of intestinal villus frequently extended their cellular bodies into the intraepithelial space. The number of total and eosinophil-specific granules was positively correlated with the minor axis of the nuclear holes in the annular nuclei. These data suggest that ileal eosinophils exhibit not homogenous but rather diverse characteristics, possible due to the mixture of eosinophils at different maturation and/or activation stages.

    Sep. 2020, Cell and tissue research, 381 (3), 439 - 450, English, International magazine

    Scientific journal

  • Satoki Nakanishi, Youhei Mantani, Tomohiro Haruta, Toshifumi Yokoyama, Nobuhiko Hoshi

    The comprehensive targets of innervation in the intestinal mucosa are unknown, partly because of the diversity of cell types and the complexity of the neural circuits. Herein, we investigated the comprehensive targets of neural connectivity and analyzed the precise characteristics of their contact structures in the mucosa of rat ileum. We examined target cells of neural connections and the characteristics of their contact structures by serial block-face scanning electron microscopy at four portions of the rat ileal mucosa: the apical and basal portions in the villi, and the lateral and basal portions around/in the crypts. Nerve fibers were in contact with several types of fibroblast-like cells (FBLCs), macrophage-like cells, eosinophils, lymphocyte-like cells, and other types of cells. The nerve fibers almost always ran more inside of lamina propria than subepithelial FBLC, and thus contacts with epithelial cells were very scarce. The contact structures of the nerve fibers were usually contained synaptic vesicle-like structures, and we classified them into patterns based on the number of nerve fiber contacting the target cells at one site, the maximum diameter of the contact structures, and the relationship between nerve fibers and nerve bundles. The contact structures for each type of cells occasionally dug into the cellular bodies of the target cells. We revealed the comprehensive targets of neural connectivity based on the characteristics of contact structures, and identified FBLCs, immunocompetent cells, and eosinophils as the candidate targets for innervation in the rat ileal mucosa.

    31 Jul. 2020, The Journal of veterinary medical science, 82 (7), 990 - 999, English, Domestic magazine

    Scientific journal

  • Establishment of an Organ Culture System to Induce Sertoli Cell Differentiation From Undifferentiated Mouse Gonads

    Chinatsu Hasegawa, Toshifumi Yokoyama, Yuria Umemura, Kohei Kawanishi, Yuuka Miura, Nanako Takada, Shuji Ohno, Kanoko Onaru, Takuya Omotehara, Tetsushi Hirano, Yohei Mantani, Takanori Miki, Nobuhiko Hoshi

    Apr. 2020, J. Vet. Med. Sci., 82 (4), 414 - 421, English

    [Refereed]

    Scientific journal

  • Shuji Ohno, Yoshinori Ikenaka, Kanoko Onaru, Shizuka Kubo, Nanami Sakata, Tetsushi Hirano, Youhei Mantani, Toshifumi Yokoyama, Keisuke Takahashi, Keisuke Kato, Koji Arizono, Takahiro Ichise, Shouta M M Nakayama, Mayumi Ishizuka, Nobuhiko Hoshi

    Neonicotinoids (NNs), a widely used class of systemic pesticides, are regarded as exhibiting selective toxicity in insects. However, NNs are suspected of exerting adverse effects on mammals as well, including humans. To date, only adult male animal models have been subjected to general toxicity studies of NNs; fetuses have yet to be considered in this context. Here, we focused on the NN clothianidin (CLO) for the first quantitative LC-MS/MS analysis of maternal-to-fetal transfer and residual property of once-daily (single or multiple days), orally administered CLO and its metabolites in mice. The results revealed the presence of CLO and its five metabolites at approximately the same respective blood levels in both dams and fetuses. In the dams, CLO showed a peak value 1 h after administration, after which levels rapidly decreased at 3 and 6 h. In the fetuses of each group, levels of CLO were almost the same as those observed in the corresponding dams. The present results clearly demonstrated rapid passage of CLO through the placental barrier. However, metabolite-dependent differences observed in blood pharmacokinetics and residual levels. This is the first quantitative demonstration of the presence of CLO and its metabolites in fetal mouse blood.

    01 Apr. 2020, Toxicology letters, 322, 32 - 38, English, International magazine

    [Refereed]

    Scientific journal

  • Kanoko Onaru, Shuji Ohno, Shizuka Kubo, Satoki Nakanishi, Tetsushi Hirano, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi

    Neonicotinoid pesticides (NNs) act as agonists on nicotinic acetylcholine receptors (nAChRs) of insects, and there have been concerns about the effects of NNs on the health of mammals. Since nAChRs are expressed in immune cells, it is possible that NNs disturb the immune system. However, few reports have examined the immunotoxicity of clothianidin (CLO), a widely-used NN. Here, we report the effects of CLO on immune organs and type IV allergic reactions in ear auricles. We orally administered CLO at 0, 30 and 300 mg/kg/day (CLO-0, 30 and 300) to Sprague-Dawley rats for 28 days. The effects were evaluated by organ and body weights, histopathology, and immunohistochemistry (TCRαβ, CD4, CD8, CD11b, CD68, CD103). In addition, some cecal contents were subjected to preliminary gut microbiota analysis, because microbiota contribute to host homeostasis, including the immunity. Our results showed loose stool, suppression of body weight gain, significant changes in organ weights (thymus: decreased; liver: increased) and changes of the gut microbiota in the CLO-300 group. There were no obvious histopathological changes in immune organs. Granulomas of the ear auricles were found in one rat of each of the CLO-30 and 300 groups, but CLO had no apparent effect on the thickness or immunohistochemistry in the ear auricles. We present new evidence that CLO affects the thymus and intestine, and might enhance the local inflammatory response. These findings should contribute to the appropriate evaluation of the safety of NNs in the future.

    24 Mar. 2020, The Journal of veterinary medical science, 82 (3), 360 - 372, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Tadashi Takada, Naoki Yoneda, Tetsushi Hirano, Kanoko Onaru, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Collins Nimako, Mayumi Ishizuka, Yoshinori Ikenaka, Nobuhiko Hoshi

    Dinotefuran (DIN) belongs to the neonicotinoids (NNs), a class of globally applied pesticides originally developed to exhibit selective toxicity in insects. However, several reports have suggested that NNs also exert neurotoxic effects in mammals. We previously demonstrated neurobehavioral effects of DIN on mice under non-stressful conditions. For further toxicity assessments in the present study, we investigated the effects of DIN on mice exposed to stressful conditions. After subacutely administering a no-observed-effect-level (NOEL) dose of DIN and/or chronic unpredictable mild stress (CUMS) to mice, we conducted three behavioral tests (i.e., open field test [OFT], tail suspension test [TST] and forced swimming test [FST]). In addition, serotonin (5-HT) and tryptophan hydroxylase 2 (TPH2) of the dorsal raphe nuclei (DRN) and median raphe nuclei (MRN) and tyrosine hydroxylase (TH) of the ventral tegmental area and substantia nigra (SN) were evaluated immunohistochemically. A NOEL dose of DIN or CUMS alone increased of the total distance in OFT, decreased or increased the immobility time in TST or FST, respectively, and increased the positive intensity of 5-HT and TPH2 in the DRN/MRN, and TH in the SN. These changes were suppressed under the conditions of combined exposure to DIN and CUMS, though the blood corticosterone level was increased depending on the blood DIN values and the presence of CUMS. The present study suggests the multifaceted toxicity of the neurotoxin DIN.

    24 Mar. 2020, The Journal of veterinary medical science, 82 (3), 350 - 359, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Toshifumi Yokoyama, Yuuka Miura, Anzu Yamamoto, Chinatsu Hasegawa, Kohei Kawanishi, Nanako Takada, Takuya Omotehara, Tetsushi Hirano, Yohei Mantani, Takanori Miki, Nobuhiko Hoshi

    C57BL/6J-XYPOS (B6J-XYPOS) mice, which have the Y chromosome derived from Mus musculus poschiavinus on a B6J genetic background, form ovotestes or ovaries. Previously, we replaced the genetic background of B6J-XYPOS mice with B6N and found that individuals with testes also appeared in addition to those with ovaries or ovotestes. To investigate the effect of the B6J genetic sequence on the testis differentiation, the genetic background of B6N-XYPOS mice was replaced with B6J again. The recovery of the B6J genetic background significantly decreased the incidence of testes; only ovaries developed. These results indicate that the testicular differentiation process tends to be perturbed especially in the B6J substrain. This shows the importance of substrain differences in mice usually treated as B6 collectively.

    27 Apr. 2019, The Journal of veterinary medical science, 81 (4), 608 - 611, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Hideto Yuasa, Youhei Mantani, Kazuki Miyamoto, Miho Nishida, Masaya Arai, Hiroki Tsuruta, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The effect of bacterial colonies expanded into the intervillous spaces on the localization of several lymphocyte lineages was immunohistochemically investigated in two types of mucosa: ordinary mucosa of rat ileum, which consists of mucosa without any mucosal lymphatic tissue; and follicle-associated mucosa (FAM), which accompanies the parafollicular area under the muscularis mucosae in the rat ileal Peyer's patch. The results showed that bacterial colonies in the intervillous spaces induced increased populations of CD8+ cells in the epithelium of the intestinal villus in ordinary mucosa (IV) and intestinal villus in FAM (IV-FAM). Bacterial colonies in the intervillous spaces were also associated with increased numbers of IgA+ cells, which were mainly localized in the lamina propria of basal portions of IV and IV-FAM, and with expanded localization of IgA+ cells into the villous apex in both IV and IV-FAM. Moreover, IgA+ cells around the intestinal crypts adjacent to IV or IV-FAM were also increased in response to bacterial colonies. In the IV-FAM, but not IV, L-selectin+ cells, which were found to be immunopositive for TCRαβ or CD19, were drastically increased in the lamina propria from the crypt to middle portion of IV-FAM and in the lumen of central lymph vessel of IV-FAM in response to the bacterial colonies in the intervillous spaces. These findings revealed that the expansion of bacterial colonies into the intervillous spaces accompanies the change of histological localization of the lymphocyte lineage in both the ordinary mucosa and FAM.

    16 Apr. 2019, The Journal of veterinary medical science, 81 (4), 555 - 566, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Youhei Mantani, Tomohiro Haruta, Miho Nishida, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Serial block-face scanning electron microscopy (SBF-SEM) is useful for three-dimensional observation of tissues or cells at high-resolution. In this study, SBF-SEM was used to three-dimensionally analyze the characteristics of fibroblast-like cells (FBLCs) in the rat ileal lamina propria (LP). The results revealed that FBLCs in LP could be divided into four types, tentatively named FBLC type I-IV, based on the external cellular appearance, abundance or shape of each organelle, detailed distribution in the LP and relationship with surrounding cells. FBLC-I and -II localized around the intestinal crypt (InC), FBLC-III localized from the lateral portion of InC to the apical portion of the intestinal villus (InV), and FBLC-IV localized in the InV. FBLC-I, -II and -III, but not FBLC-IV, localized beneath the epithelium. FBLC-II possessed thin lamellar-shaped endoplasmic reticula, whereas FBLC-I possessed expanded endoplasmic reticula that occasionally showed a spherical shape. FBLC-III and -IV possessed a cytoplasmic region with high-electron density and no organelles immediately beneath the cellular membrane; this region was found at the epithelial sides in FBLC-III and scattered in FBLC-IV. FBLC-IV were in constant close proximity to villous myocytes throughout the InV and also in contact with FBLC-III especially in the apical portion of the InV. FBLC-I, -II and -IV, but not -III, were constantly found to be in contact with various immunocompetent cells in the LP. Three-dimensional analysis using SBF-SEM indicates that four types of FBLC localized in the rat ileal LP.

    30 Mar. 2019, The Journal of veterinary medical science, 81 (3), 454 - 465, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Toshifumi Yokoyama, Takuya Omotehara, Tetsushi Hirano, Naoto Kubota, Shogo Yanai, Chinatsu Hasegawa, Tadashi Takada, Yohei Mantani, Nobuhiko Hoshi

    Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.

    01 Nov. 2018, The Journal of veterinary medical science, 80 (10), 1534 - 1539, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Youhei Mantani, Miho Nishida, Kyouji Yamamoto, Kazuki Miyamoto, Hideto Yuasa, Natsumi Masuda, Takuya Omotehara, Hiroki Tsuruta, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Paneth cells secrete bactericidal substances in response to bacterial proliferation on the mucosal surface without directly contacting bacteria. However, the induction mechanism of this transient secretion has not been clarified, although nervous system and/or immunocompetent cells in the lamina propria (LP) might be involved. In this study, we ultrastructurally and immunohistochemically investigated which LP cells are localized beneath Paneth cells and examined the relationship between the Paneth cell-derived cellular processes which extended into the LP and the LP cells. The results showed that various cells-including blood capillary, subepithelial stromal cell, and nerve fiber-were present in the LP beneath Paneth cells. Endothelial cells of blood capillary were the cells most frequently found in this location; they were situated within 1 μm of the Paneth cells and possessed fenestration on the surfaces adjacent to Paneth cells. The Paneth cells rarely extended the cellular processes toward the LP across the basal lamina. Most of the cellular processes of Paneth cells contacted the subepithelial stromal cells. Immunohistochemistry revealed that the CD34+ CD31- αSMA- stromal cells preferentially localized in the LP beneath the intestinal crypt base, while PDGFRαhi αSMA+ stromal cells mainly localized around the lateral portions of the intestinal crypt and PDGFRαhi αSMA- stromal cells localized in the intestinal villus. From these findings, the existence of blood capillaries beneath Paneth cells might reflect the active exocrine function of Paneth cells. Furthermore, subepithelial stromal cells, probably with a CD34+ CD31- αSMA- PDGFRα-/lo phenotype, beneath the crypt base might affect Paneth cell activity by interacting with their cellular processes. Anat Rec, 301:1074-1085, 2018. © 2018 Wiley Periodicals, Inc.

    Jun. 2018, Anatomical record (Hoboken, N.J. : 2007), 301 (6), 1074 - 1085, English, International magazine

    [Refereed]

    Scientific journal

  • Tadashi Takada, Naoki Yoneda, Tetsushi Hirano, Shogo Yanai, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Nobuhiko Hoshi

    It has been suggested that an increase in the use of pesticides affects neurodevelopment, but there has been no animal experiment showing a causal relation between neonicotinoid pesticides (NNs) and depression. We examined whether dinotefuran (DIN), the most widely used NN in Japan, induces depression. Male mice were administered DIN between 3 and 8 weeks of age, referring to the no-observed-effect level (NOEL). The mice were then subjected to a tail suspension test (TST) and a forced swimming test (FST). After these tests, their brains were dissected for immunohistochemical analyses of serotonin (5-HT). Antidepressant activity in TST and no decrease in 5-HT-positive cells were observed. The subchronic exposure to DIN alone in juvenile male mice may not cause depression-like indication.

    Japanese Society of Veterinary Science, 27 Apr. 2018, The Journal of veterinary medical science, 80 (4), 720 - 724, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Anzu Yamamoto, Takuya Omotehara, Yuuka Miura, Tadashi Takada, Naoki Yoneda, Tetsushi Hirano, Youhei Mantani, Hiroshi Kitagawa, Toshifumi Yokoyama, Nobuhiko Hoshi

    Anti-Müllerian hormone (AMH) produced in the developing testis induces the regression of the Müllerian duct, which develops into the oviducts, uterus and upper vagina. In our true hermaphrodite mouse with an ovary on one side and a testis on the other (O/T), the oviduct and uterus are present only on the ovary side, and nothing derived from the Müllerian duct is present on the testis side. Here, we investigate the mechanism underlying the unilateral Müllerian duct regression and the mode of AMH signaling, by performing immunohistology, Western blotting, and organ culture analyses. The histological analysis revealed that during the start of the Müllerian duct regression, the duct in the O/T mice was clearly regressed on the AMH-positive testis side compared to the AMH-negative ovary side. The immunohistochemistry showed a diffuse immunoreaction of AMH in the interstitium surrounding the testis cord and boundary region between the testis and mesonephros, especially in the cranial portion. Western blotting revealed that the amount of AMH in the cranial half of the mesonephros was larger than that in the caudal half. AMH injected into the gonads in organ culture induced the regression of the Müllerian duct via the interstitium of the organ. These results suggest that AMH acts on the Müllerian duct in male mice by exuding into the interstitium surrounding the testis cord and infiltrating through the cranial region from the testis to the mesonephros.

    18 Apr. 2018, The Journal of veterinary medical science, 80 (4), 557 - 567, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Naoki Yoneda, Tadashi Takada, Tetsushi Hirano, Shogo Yanai, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Nobuhiko Hoshi

    Although neonicotinoid pesticides are expected to have harmful influence on mammals, there is little animal experimental data to support the effect and mechanisms. Since acetylcholine causes the release of dopamine, neonicotinoids may confer a risk of developmental disorders via a disturbance in the monoamine systems. Male mice were peripubertally administered dinotefuran (DIN) referring to no observed effect level (NOEL) and performed behavioral and immunohistological analyses. In an open field test, the total locomotor activity was increased in a dose-dependent manner. The immunoreactivity of tyrosine hydroxylase in the substantia nigra was increased in DIN-exposed mice. These results suggest that exposure to DIN in peripubertal male mice causes hyperactivity and a disturbance of dopaminergic signaling.

    Japanese Society of Veterinary Science, 18 Apr. 2018, The Journal of veterinary medical science, 80 (4), 634 - 637, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Natsumi Masuda, Youhei Mantani, Hideto Yuasa, Chiaki Yoshitomi, Masaya Arai, Miho Nishida, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The distributions of β-defensin 1 and 2 in secretory host defense system throughout respiratory tract of healthy rats were immunohistochemically investigated. In the nasal epithelium, a large number of non-ciliated and non-microvillous cells (NCs) were immunopositive for both β-defensin 1 and 2, whereas a small number of goblet cells (GCs) were immunopositive only for β-defensin 1. Beta-defensin 2-immunopositive GCs were few. In the nasal glands, a small number of acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins. In the laryngeal and tracheal epithelia, a very few NCs and GCs were immunopositive for both β-defensins. In laryngeal and tracheal glands, a very few acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins. In the extra-pulmonary bronchus, a small number of NCs were immunopositive for both β-defensins. A small number of GCs were immunopositive for β-defensin 1, whereas few GCs were immunopositive for β-defensin 2. From the intra-pulmonary bronchus to alveoli, a very few or no epithelial cells were immunopositive for both β-defensins. In the mucus and periciliary layers, β-defensin 1 was detected from the nose to the extra-pulmonary bronchus, whereas β-defensin 2 was weakly detected only in the nose and the larynx. These findings suggest that the secretory sources of β-defensin 1 and 2 are mainly distributed in the nasal mucosa and gradually decrease toward the caudal airway in healthy rats.

    Japanese Society of Veterinary Science, 24 Mar. 2018, The Journal of veterinary medical science, 80 (3), 395 - 404, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Natsumi Masuda, Youhei Mantani, Chiaki Yoshitomi, Hideto Yuasa, Miho Nishida, Masaya Arai, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The host defense system with lysozyme and secretory phospholipase A2 (sPLA2) was immunohistochemically investigated in rat respiratory tract under healthy conditions. In the nasal epithelium, a large number of non-ciliated and non-microvillous cells (NC) and a small number of goblet cells (GC) were immunopositive for lysozyme and sPLA2. A few acinar cells and almost all epithelial cells of intercalated ducts were immunopositive for both bactericidal substances in the nasal glands. In the laryngeal and tracheal epithelia, few NC and GC were immunopositive for both bactericidal substances. In the laryngeal and tracheal glands, a few acinar cells and most ductal epithelial cells were immunopositive for both bactericidal substances. In extra-pulmonary bronchus, small numbers of NC and GC were immunopositive for lysozyme and sPLA2, whereas few NC and no GC were immunopositive in the intra-pulmonary bronchus. No secretory source of either bactericidal substance was located in the bronchioles. In the alveolus, many glandular epithelial cells and alveolar macrophages were immunopositive for lysozyme but immunonegative for sPLA2. Moreover, lysozyme and sPLA2 were detected in the mucus layer and in the periciliary layer from the nose to the extra-pulmonary bronchus. These findings suggest that secretory sources of lysozyme and sPLA2 are distributed in almost all the respiratory tract. Their secretory products are probably transported to the pharynx and contribute to form the first line of defense against inhaled bacteria throughout the respiratory tract.

    Japanese Society of Veterinary Science, 02 Mar. 2018, The Journal of veterinary medical science, 80 (2), 323 - 332, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Tetsushi Hirano, Shogo Yanai, Tadashi Takada, Naoki Yoneda, Takuya Omotehara, Naoto Kubota, Kiichi Minami, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

    Neonicotinoids are novel systemic pesticides acting as agonists on the nicotinic acetylcholine receptors (nAChRs) of insects. Experimental studies have revealed that neonicotinoids pose potential risks for the nervous systems of non-target species, but the brain regions responsible for their behavioral effects remain incompletely understood. This study aimed to assess the neurobehavioral effects of clothianidin (CTD), a later neonicotinoid developed in 2001 and widely used worldwide, and to explore the target regions of neonicotinoids in the mammalian brain. A single-administration of 5 or 50mg/kg CTD to male C57BL/6N mice at or below the no-observed-adverse-effect level (NOAEL) induced an acute increase in anxiety during the elevated plus-maze test. In addition, mice in the CTD-administered group spontaneously emitted human-audible vocalizations (4-16kHz), which are behavioral signs of aversive emotions, and showed increased numbers of c-fos immunoreactive cells in the paraventricular thalamic nucleus and dentate gyrus of the hippocampus. In conclusion, mice exposed to NOAEL-dose CTD would be rendered vulnerable to a novel environment via the activation of thalamic and hippocampal regions related to stress responses. These findings should provide critical insight into the neurobehavioral effects of neonicotinoids on mammals.

    ELSEVIER IRELAND LTD, 05 Jan. 2018, Toxicology letters, 282, 57 - 63, English, International magazine

    [Refereed]

    Scientific journal

  • Hideto Yuasa, Youhei Mantani, Natsumi Masuda, Miho Nishida, Masaya Arai, Toshifumi Yokoyama, Hiroki Tsuruta, Junichi Kawano, Nobuhiko Hoshi, Hiroshi Kitagawa

    The mechanism by which indigenous bacteria on the follicle-associated epithelium (FAE) of lymphatic follicles (LFs) accelerate the differentiation of microvillous columnar epithelial cells (MV) into M-cells was immunohistochemically investigated in rat Peyer's patches. The results showed that the number of Toll-like receptor (TLR) -4+ M-cells was greater in the FAE with expansion of bacterial colonies (LFs with bacterial colonies on the FAE: b-LF) than the FAE without expansion of bacterial colonies (nb-LF). TLR-4 was also expressed in the striated borders of MV upstream next to M-cells in the FAE of the b-LF. TLR-4+ vesicles were frequently detected in the cytoplasms of MV with TLR-4+ striated borders upstream next to TLR-4+ M-cells in the FAE of b-LF. These findings suggest that TLR-4+ MV take up TLR-4 ligands and differentiate into M-cells in the b-LF. Neither the distribution of RANK nor that of RANKL was coincident with that of M-cells in the b-LF. Moreover, RANK, but not RANKL, was expressed in intestinal villi, whereas cleaved caspase-3 was immunonegative in the MV and M-cells of the FAE, unlike in villous epithelial cells. Therefore, RANK/RANKL signaling in the LF might contribute to the down-regulation of epithelial apoptosis to facilitate the differentiation of MV into M-cells in rat Peyer's patches.

    JAPAN SOC VET SCI, 17 Nov. 2017, The Journal of veterinary medical science, 79 (11), 1826 - 1835, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Shogo Yanai, Tetsushi Hirano, Takuya Omotehara, Tadashi Takada, Naoki Yoneda, Naoto Kubota, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

    Neonicotinoids are pesticides used worldwide. They bind to insect nicotinic acetylcholine receptors (nAChRs) with high affinity. We previously reported that clothianidin (CTD), one of the latest neonicotinoids, reduced antioxidant expression and induced germ cell death in the adult testis of vertebrates. Here, we investigated the male reproductive toxicity of prenatal and early postnatal exposure to CTD, because it is likely that developmental exposure more severely affects the testis compared to adults due to the absence of the blood-testis barrier. Pregnant C57BL/6 mice were given water gel blended with CTD (0, 10 or 50 mg/kg/day; no-observed-adverse-effect-level [NOAEL for mice]: 47.2 mg/kg/day) between gestational day 1 and 14 days post-partum. We then examined the testes of male offspring at postnatal day 14. The testis weights and the numbers of germ cells per seminiferous tubule were decreased in the CTD-50 group, and abnormal tubules containing no germ cells appeared. Nevertheless, the apoptotic cell number and proliferative activity were not significantly different between the control and CTD-exposed groups. There were no significant differences in the androgen-related parameters, such as the Leydig cell volume per testis, the Sertoli cell number and the tubule diameter. The present study is the first demonstration that in utero and lactational exposures to CTD at around the NOAEL for mice reduce the germ cell number, but our findings suggest that these exposures do not affect steroidogenesis in Leydig cells during prenatal or early postnatal life.

    JAPAN SOC VET SCI, 07 Jul. 2017, The Journal of veterinary medical science, 79 (7), 1196 - 1203, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Takuya Omotehara, Kiichi Minami, Youhei Mantani, Yuria Umemura, Miho Nishida, Tetsushi Hirano, Hidefumi Yoshioka, Hiroshi Kitagawa, Toshifumi Yokoyama, Nobuhiko Hoshi

    BACKGROUND: The left male gonad in the chicken embryo has a thickened cortical layer, but it eventually becomes flattened after the onset of testicular development. Because the destination of the cortical cells migrating from the left gonad remains unclear, we examined this issue herein. RESULTS: The testis-inducing gene doublesex- and mab-3-related transcription factor 1 (DMRT1) was detected in a proportion of the columnar and cubic epithelial cells in the cortex of the left testis as well as Sertoli cells in both testes. Interestingly, some of the DMRT1-expressing cortical cells were contiguous with Sertoli cells in the testis cord. Some cortical cells exhibited a vimentin-positive cytoplasm that was elongated all the way to the medulla. In addition, a desmosome-like structure was observed between the elongated cytoplasm in these cells and the adjacent Sertoli cell. After the organ culture, a few cells labeled with a fluorescent dye that stained only the cortical cells at the beginning of the culture were located in the testis cord of the left testis. CONCLUSIONS: Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148-156, 2017. © 2016 Wiley Periodicals, Inc.

    WILEY, Feb. 2017, Developmental dynamics : an official publication of the American Association of Anatomists, 246 (2), 148 - 156, English, International magazine

    [Refereed]

    Scientific journal

  • Hideto Yuasa, Youhei Mantani, Natsumi Masuda, Miho Nishida, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The expressions of Toll-like receptor (TLR) -2, -4 and -9 were immunohistochemically investigated in the follicle-associated epithelium (FAE), and epithelia of the follicle-associated intestinal villus (FAIV) and ordinary intestinal villus (IV) in rat Peyer's patch regions with no bacterial colonies on the mucous membranes. TLR-2 was expressed in the striated borders of microvillous columnar epithelial cells (MV) in both FAIV and IV except in the apices. However, TLR-2 expression in the striated borders was weaker in the epithelium of the follicular side of FAIV (f-FAIV) than in epithelia of IV and the anti-follicular side of FAIV. TLR-4 and -9 were not expressed in the FAIV and IV. In the FAE, TLR-2, -4 and -9 were not expressed in the striated borders of MV, but the roofs of some typical M-cells were immunopositive for all TLRs. Especially, no TLR-positive MV were found at the FAE sites where M-cells appeared most frequently. In the follicle-associated intestinal crypt (FAIC), immunopositivity for all TLRs was observed in the striated borders of MV and the luminal substances. In conclusion, the lower levels of TLR-2 in both FAE and the epithelium of f-FAIV probably reduce recognition of indigenous bacteria. TLR-2, -4 and -9 appear not to participate directly in differentiation of MV into M-cells, because TLRs were not expressed in any MV in the upstream region of M-cells in FAE with no settlement of indigenous bacteria in the rat Peyer's patches.

    JAPAN SOC VET SCI, 10 Jan. 2017, The Journal of veterinary medical science, 78 (12), 1797 - 1804, English, Domestic magazine

    [Refereed]

    Scientific journal

  • ネオニコチノイド系農薬曝露によりみられた神経行動学的異常および標的脳領域の探索

    平野 哲史, 柳井 翔吾, 高田 匡, 米田 直起, 表原 拓也, 久保田 直人, 南 貴一, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2016, 環境ホルモン学会研究発表会要旨集, 19回, 63 - 63, Japanese

    [Refereed]

  • ネオニコチノイド系農薬クロチアニジンは胎子型と成獣型、2種類のライディッヒ細胞にそれぞれ異なる影響を及ぼす

    柳井 翔吾, 平野 哲史, 表原 拓也, 高田 匡, 米田 直起, 岩本 遥, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2016, 環境ホルモン学会研究発表会要旨集, 19回, 67 - 67, Japanese

    [Refereed]

  • 胎子期および発達期におけるネオニコチノイド系農薬曝露がドーパミン神経系および行動に及ぼす影響

    米田 直起, 平野 哲史, 柳井 翔吾, 高田 匡, 岩本 遥, 表原 拓也, 片田 裕亮, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2016, 環境ホルモン学会研究発表会要旨集, 19回, 101 - 101, Japanese

    [Refereed]

  • ネオニコチノイド系農薬ジノテフラン曝露はうつ様行動を引き起こすか?

    高田 匡, 平野 哲史, 柳井 翔吾, 米田 直起, 岩本 遥, 表原 拓也, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2016, 環境ホルモン学会研究発表会要旨集, 19回, 102 - 102, Japanese

    [Refereed]

  • マウス未分化性腺におけるSry発現制御に関わる新規遺伝子の時空間特異的探索

    梅村 ゆりあ, 横山 俊史, 田渕 圭章, 南 貴一, 表原 拓也, 久保田 直人, 山本 杏, 広川 千英, 平野 哲史, 柳井 翔吾, 米田 直起, 高田 匡, 万谷 洋平, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2016, 日本獣医学会学術集会講演要旨集, 159回, 312 - 312, Japanese

    [Refereed]

  • マウス真性半陰陽個体におけるAMHの作用様式に関する組織学的解析

    山本 杏, 表原 拓也, 久保田 直人, 南 貴一, 広川 千英, 平野 哲史, 磯江 美智子, 柳井 翔吾, 高田 匡, 米田 直起, 三浦 由佳, 岩本 遥, 万谷 洋平, 北川 浩, 横山 俊史, 星 信彦

    (公社)日本獣医学会, Aug. 2016, 日本獣医学会学術集会講演要旨集, 159回, 312 - 312, Japanese

    [Refereed]

  • ネオニコチノイド系農薬クロチアニジン急性投与が及ぼす行動学的影響とその作用点

    平野 哲史, 柳井 翔吾, 高田 匡, 米田 直起, 表原 拓也, 久保田 直人, 南 貴一, 山本 杏, 岩本 遥, 三浦 由佳, 磯江 美智子, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2016, 日本獣医学会学術集会講演要旨集, 159回, 314 - 314, Japanese

    [Refereed]

  • ネオニコチノイド系農薬ジノテフランによるドーパミン神経系の攪乱および多動性の誘発

    米田 直起, 平野 哲史, 柳井 翔吾, 高田 匡, 表原 拓也, 久保田 直人, 南 貴一, 山本 杏, 岩本 遥, 三浦 由佳, 磯江 美智子, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2016, 日本獣医学会学術集会講演要旨集, 159回, 315 - 315, Japanese

    [Refereed]

  • ネオニコチノイド系農薬とうつ発症との関連性の検証

    高田 匡, 平野 哲史, 柳井 翔吾, 米田 直起, 表原 拓也, 久保田 直人, 南 貴一, 山本 杏, 岩本 遥, 三浦 由佳, 磯江 美智子, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2016, 日本獣医学会学術集会講演要旨集, 159回, 315 - 315, Japanese

    [Refereed]

  • ネオニコチノイド系農薬クロチアニジン単回投与によりみられた異常啼鳴および神経行動学的影響

    平野 哲史, 柳井 翔吾, 高田 匡, 米田 直起, 表原 拓也, 久保田 直人, 南 貴一, 広川 千英, 山本 杏, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (一社)日本毒性学会, Jun. 2016, The Journal of Toxicological Sciences, 41 (Suppl.), S339 - S339, Japanese

    [Refereed]

  • HIRANO Tetsushi, MANNTANI Youhei, YOKOYAMA Toshifumi, KITAGAWA Hiroshi, HOSHI Nobuhiko, YANAI Shogo, TAKADA Tadashi, YONEDA Naoki, OMOTEHARA Takuya, KUBOTA Naoto, MINAMI Kiichi, HIROKAWA Chiaki, YAMAMOTO Anzu

    【背景】ネオニコチノイドは1990年代に昆虫型ニコチン性アセチルコリン受容体(nAChRs)を標的として開発された農薬成分である.しかし近年,ネオニコチノイドは哺乳類型nAChRsを介して神経細胞に興奮反応を引き起こすことが示され,昆虫以外の生物に対して不測の影響を与える可能性が懸念されている.我々はこれまでに成熟雄マウスをネオニコチノイドの1種,クロチアニジン(CTD)に4週間曝露すると,新規環境(オープンフィールド)における不安様行動が引き起こされ,その影響は環境ストレス下においてより顕在化することを報告してきた.本研究では,ネオニコチノイドによる行動影響発現に関与する脳領域を明らかにすることを目的とした.
    【材料と方法】C57BL/6成熟雄マウスに5または50 mg/kgの CTDを単回経口投与し,投与1時間後に高架式十字迷路試験による行動解析を行った.さらに2時間後に脳を摘出し,c-fos発現を指標とした組織学的解析により投与後誘導された神経活動を評価した.
    【結果と考察】行動解析の結果,CTD 5 mg/kg投与群においては溶媒投与対照群と比較してOpen arm滞在時間および侵入回数の減少がみられた.CTD 50 mg/kg投与群においては,さらに総移動距離の減少ならびに迷路探索時における異常啼鳴(Abnormal vocalization)およびすくみ行動(Freezing)が観察された.組織学的解析の結果,情動およびストレス反応に関与する視床下部,海馬においてc-fos陽性細胞数の増加がみられた.
    以上の結果から,CTD投与下においては,新規環境ストレスに曝露された際にコリン作動性神経投射を受ける視床下部や海馬における過剰な神経興奮が生じ,不安様行動やストレス応答を濃度依存的に誘発する可能性が示唆された.

    The Japanese Society of Toxicology, 2016, Annual Meeting of the Japanese Society of Toxicology, 43 (0), P - 252, Japanese

    [Refereed]

  • Yuria Umemura, Ryosuke Miyamoto, Rie Hashimoto, Kyoko Kinoshita, Takuya Omotehara, Daichi Nagahara, Tetsushi Hirano, Naoto Kubota, Kiichi Minami, Shogo Yanai, Natsumi Masuda, Hideto Yuasa, Youhei Mantani, Eiko Matsuo, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

    Mammalian sexual fate is determined by the presence or absence of sex determining region of the Y chromosome (Sry) in the "bipotential" gonads. Recent studies have demonstrated that both male and female sexual development are induced by distinct and active genetic pathways. Breeding the Y chromosome from Mus m. domesticus poschiavinus (POS) strains into C57BL/6J (B6J) mice (B6J-XY(POS)) has been shown to induce sex reversal (75%: bilateral ovary, 25%: true hermaphrodites). However, our B6N-XY(POS) mice, which were generated by backcrossing of B6J-XY(POS) on an inbred B6N-XX, develop as males (36%: bilateral testis with fertility as well as bilateral ovary (34%), and the remainder develop as true hermaphrodites. Here, we investigated in detail the expressions of essential sex-related genes and histological features in B6N-XY(POS) mice from the fetal period to adulthood. The onsets of both Sry and SRY-box 9 (Sox9) expressions as determined spatiotemporally by whole-mount immunohistochemistry in the B6N-XY(POS) gonads occurred 2-3 tail somites later than those in B6N-XY(B6) gonads, but earlier than those in B6J-XY(POS), respectively. It is possible that such a small difference in timing of the Sry expression underlies testicular development in our B6N-XY(POS). Our study is the first to histologically show the expression and ectopic localization of a female-related gene in the XY(POS) testes and a male-related gene in the XY(POS) ovaries. The results from these and previous experiments indicate that the interplay between genome variants, epigenetics and developmental gene regulation is crucial for testis development.

    JAPAN SOC VET SCI, Jan. 2016, The Journal of veterinary medical science, 77 (12), 1587 - 98, English, Domestic magazine

    [Refereed]

    Scientific journal

  • ネオニコチノイド系農薬クロチアニジンの妊娠期及び授乳期曝露がマウス産子の雄性生殖器に与える影響

    柳井 翔吾, 平野 哲史, 表原 拓也, 高田 匡, 米田 直起, 梅村 ゆりあ, 久保田 直人, 南 貴一, 広川 千英, 山本 杏, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2015, 環境ホルモン学会研究発表会要旨集, 18回, 109 - 109, Japanese

    [Refereed]

  • 環境ストレス下においてネオニコチノイド系農薬クロチアニジンが与える神経行動学的影響

    平野 哲史, 柳井 翔吾, 表原 拓也, 高田 匡, 米田 直起, 梅村 ゆりあ, 久保田 直人, 南 貴一, 広川 千英, 山本 杏, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2015, 環境ホルモン学会研究発表会要旨集, 18回, 118 - 118, Japanese

    [Refereed]

  • ニワトリ胚の左精巣に特異的なセルトリ細胞の起源

    表原 拓也, 万谷 洋平, 南 貴一, 梅村 ゆりあ, 平野 哲史, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本生化学会, Dec. 2015, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [2P0993] - [2P0993], Japanese

    [Refereed]

  • Yoshihiro Kobayashi, Tetsushi Hirano, Takuya Omotehara, Rie Hashimoto, Yuria Umemura, Hideto Yuasa, Natsumi Masuda, Naoto Kubota, Kiichi Minami, Shogo Yanai, Mitsuko Ishihara-Sugano, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

    Dioxins are widespread persistent environmental contaminants with adverse impacts on humans and experimental animals. Behavioral and cognitive functions are impaired by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure. TCDD exerts its toxicity via the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor. The hippocampus, which plays important roles in episodic memory and spatial function, is considered vulnerable to TCDD-induced neurotoxicity, because it contains the AhR. We herein investigated the effects of TCDD toxicity on hippocampal development in embryonic mice. TCDD was administered to dams at 8.5 days postcoitum with a single dose of 20, 200, 2,000 and 5,000 ng/kg body weight (groups T20, T200, T2000 and T5000, respectively), and the brains were dissected from their pups at embryonic day 18.5. Immunohistochemical analysis demonstrated that the Glial Fibrillary Acidic Protein (GFAP) immunoreactivities in the dentate gyrus (DG) were reduced in the T5000 group. Granular GFAP immunoreactivity was observed in the hippocampal fimbria, and the number of immunoreactive fimbria was significantly decreased in the T5000 group. The number of Proliferating Cell Nuclear Antigen (PCNA)-positive cells was decreased in all TCDD-exposed groups and significantly reduced in the T20, T200 and T5000 groups. Together, these results demonstrate that maternal TCDD exposure has adverse impacts on neural stem cells (NSCs), neural precursor cells (NPCs) and granular cells in the DG and disrupts the NSC maintenance and timing of differentiation in the hippocampal fimbria, which in turn interrupt neuronal development in future generations of mice.

    JAPAN SOC VET SCI, Nov. 2015, The Journal of veterinary medical science, 77 (11), 1355 - 61, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Tetsushi Hirano, Shogo Yanai, Takuya Omotehara, Rie Hashimoto, Yuria Umemura, Naoto Kubota, Kiichi Minami, Daichi Nagahara, Eiko Matsuo, Yoshiko Aihara, Ryota Shinohara, Tomoyuki Furuyashiki, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

    Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling cause of honeybee colony collapse disorder, which is damaging crop production that requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day), for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test, although the locomotor activities were not affected, the anxiety-like behaviors of the mice were elevated by both CTD and stress. The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

    JAPAN SOC VET SCI, Oct. 2015, The Journal of veterinary medical science, 77 (10), 1207 - 15, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Youhei Mantani, Eri Ito, Miho Nishida, Hideto Yuasa, Natsumi Masuda, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Indigenous bacteria in the alimentary tract are exposed to various bactericidal peptides and digestive enzymes, but the viability status and morphological changes of indigenous bacteria are unclear. Therefore, the present study aimed to ultrastructurally clarify the degeneration and viability status of indigenous bacteria in the rat intestine. The majority of indigenous bacteria in the ileal mucous layer possessed intact cytoplasm, but the cytoplasm of a few bacteria contained vacuoles. The vacuoles were more frequently found in bacteria of ileal chyme than in those of ileal mucous layer and were found in a large majority of bacteria in both the mucous layer and chyme throughout the large intestine. In the dividing bacteria of the mucous layer and chyme throughout the intestine, the ratio of area occupied by vacuoles was almost always less than 10%. Lysis or detachment of the cell wall in the indigenous bacteria was more frequently found in the large intestine than in the ileum, whereas bacterial remnants, such as cell walls, were distributed almost evenly throughout the intestine. In an experimental control of long-time-cultured Staphylococcus epidermidis on agar, similar vacuoles were also found, but cell-wall degeneration was never observed. From these findings, indigenous bacteria in the mucous layer were ultrastructurally confirmed to be the source of indigenous bacteria in the chyme. Furthermore, the results suggested that indigenous bacteria were more severely degenerated toward the large intestine and were probably degraded in the intestine.

    JAPAN SOC VET SCI, Sep. 2015, The Journal of veterinary medical science, 77 (9), 1121 - 8, English, Domestic magazine

    [Refereed]

    Scientific journal

  • 環境ストレス下において浸透性農薬クロチアニジンが行動及び生殖機能に与える影響

    平野 哲史, 柳井 翔吾, 表原 拓也, 梅村 ゆりあ, 久保田 直人, 南 貴一, 高田 匡, 米田 直起, 広川 千英, 山本 杏, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2015, 日本獣医学会学術集会講演要旨集, 158回, 280 - 280, Japanese

    [Refereed]

  • Ei-ichirou Takahara, Hideto Yuasa, Miho Nishida, Youhei Mantani, Kankanam Gamage Sanath Udayanga, Wang-Mei Qi, Takashi Takeuchi, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    A portion of the minute chylomicrons less than 75 nm in diameter are transcytosed from the extravascular tissue into the subepithelial blood capillaries (sBC) in the villous apices of the rat jejunum. However, the details of the transportation mechanism have not been clarified. In this study, the endothelial receptor involved in the transportation of minute chylomicrons into the sBC's lumina was immunohistochemically and histoplanimetrically examined in intestinal villi of the rat jejunum. Immunopositivity for very low density lipoprotein (VLDL) receptor was detected on the luminal and basal surfaces of the endothelial cells of sBC in approximately 68% of those apices of jejunal villi that possessed numerous chylomicrons in the lamina propria, while VLDL receptor was detected on the endothelial cells of sBC in only approximately 8% of intestinal villi that possessed few or no chylomicrons in the lamina propria. No immunopositivity for LDL receptor was detected in the sBC of all intestinal villi. These findings suggest that VLDL receptor is expressed by the endothelial cells of the sBC in conjunction with the filling of the lamina propria of jejunal villi with many chylomicrons produced by the villous columnar epithelial cells and that the VLDL receptor mediates the transportation of minute chylomicrons, maybe VLDL, into the subepithelial portal blood from the extravascular tissue of the rat jejunal villi.

    JAPAN SOC VET SCI, Apr. 2015, The Journal of veterinary medical science, 77 (4), 387 - 93, English, Domestic magazine

    [Refereed]

    Scientific journal

  • ネオニコチノイド系農薬クロチアニジン及び環境ストレス複合曝露が生殖器及び行動に与える影響

    平野 哲史, 柳井 翔吾, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    環境ホルモン学会(日本内分泌撹乱化学物質学会), Dec. 2014, 環境ホルモン学会研究発表会要旨集, 17回, 132 - 132, Japanese

    [Refereed]

  • Tetsushi Hirano, Yoshihiro Kobayashi, Takuya Omotehara, Atsutoshi Tatsumi, Rie Hashimoto, Yuria Umemura, Daichi Nagahara, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

    Environmental stress affects various parts of mammals typically through the circulation of stress hormones. It has been identified as one of the possible reasons for male reproductive difficulties, but the complex mechanisms responsible for stress-induced reproductive suppression are poorly understood. Here, we examined the relationship between chronic environmental stress and hypothalamic kisspeptin, a recently discovered upstream regulator of the reproductive endocrine feedback system. We studied male mice under an unpredictable chronic stress procedure to replicate the situation of animals under chronic stress. Histological and immunohistochemical analyses were performed focusing on kisspeptin neurons in the arcuate hypothalamic nucleus (ARC) and DNA fragmented cells in seminiferous tubules. Although the ARC was not morphologically altered in either the stressed or non-stressed group, granular kisspeptin immunoreactivities decreased slightly in the stress group. In the testes of the stress group, several signs of testicular degeneration were observed, including increased numbers of ssDNA-positive cells per seminiferous tubule, thinning, vacuoled seminiferous epithelia and multinucleated giant cells. The decreases in kisspeptin in the stress group might be due to other hypothalamic peptides, such as corticotropin-releasing hormone and leptin, whose receptors are known to coexpress in the ARC. In addition, environmental stress directly and indirectly affects testicular function through stress hormones and gonadotropins. In summary, our findings enhance the understanding of stress-induced reproductive suppression possibly mediated by kisspeptin in the ARC.

    JAPAN SOC VET SCI, Sep. 2014, The Journal of veterinary medical science, 76 (9), 1201 - 8, English, Domestic magazine

    [Refereed]

    Scientific journal

  • マウス胎子生殖腺における性分化関連遺伝子発現の左右差に関する分子形態学的研究

    橋本 理恵, 梅村 ゆりあ, 表原 拓也, 平野 哲史, 長原 大知, 湯浅 秀人, 久保田 直人, 南 貴一, 柳井 翔吾, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2014, 日本獣医学会学術集会講演要旨集, 157回, 318 - 318, Japanese

    [Refereed]

  • ニワトリ胚における性腺の形成メカニズムと左右差

    表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, 久保田 直人, 柳井 翔吾, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2014, 日本獣医学会学術集会講演要旨集, 157回, 318 - 318, Japanese

    [Refereed]

  • 発生中のマウス胎子生殖腺における内部標準遺伝子の検討

    横山 俊史, 橋本 理恵, 梅村 ゆりあ, 表原 拓也, 長原 大知, 平野 哲史, 久保田 直人, 南 貴一, 柳井 翔吾, 万谷 洋平, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2014, 日本獣医学会学術集会講演要旨集, 157回, 319 - 319, Japanese

    [Refereed]

  • ネオニコチノイド系農薬クロチアニジンの低用量曝露とストレスの複合影響

    平野 哲史, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, 柳井 翔吾, 長原 大知, 湯浅 秀人, 万谷 洋平, 横山 俊史, 北川 浩, 星 信彦

    (公社)日本獣医学会, Aug. 2014, 日本獣医学会学術集会講演要旨集, 157回, 331 - 331, Japanese

    [Refereed]

  • Youhei Mantani, Miho Nishida, Hideto Yuasa, Kyouji Yamamoto, Ei-Ichirou Takahara, Takuya Omotehara, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Paneth cells (PCs) contribute to the host defense against indigenous bacteria in the small intestine. We found Paneth cell-like cells (PLCs) in the rat ascending colon, but the nature of PLCs is never clarified. Therefore, the present study aimed to clarify the cytological characteristics of PLCs and discuss their cellular differentiation. PLCs were localized in the bases of intestinal crypts, especially follicle-associated intestinal crypts in proximal colonic lymphoid tissue, but were very seldom found in the ordinary intestinal crypts of the ascending colon. PLCs possessed specific granules with highly electron-dense cores and haloes, as well as PCs in the small intestine. The secretory granules of PLCs were positive for PAS reaction, lysozyme and soluble phospholipase A2, but negative for Alcian blue staining, β-defensin-1 and -2, as well as the ones of PCs. Furthermore, intermediate cells possessing both the PLC-specific granules and the mucus granules similar to those of goblet cells (GCs) were occasionally found in the vicinity of PLCs. Intermediate cells ranged from goblet cell-like cells rich in mucus granules to PLC-like cells with few mucus granules. The cellular condensation and fragmentation were exclusively found in PLCs but never seen in intermediate cells or GCs. The PLCs, which were identified as PC, were suggested to be transformed from GCs through intermediate cells and finally to die by apoptosis in intestinal crypts of proximal colonic lymphoid tissue in the rat ascending colon.

    Aug. 2014, Anatomical record (Hoboken, N.J. : 2007), 297 (8), 1462 - 71, English, International magazine

    [Refereed]

    Scientific journal

  • Youhei Mantani, Hideto Yuasa, Miho Nishida, Ei-ichirou Takahara, Takuya Omotehara, Kankanam Gamage Sanath Udayanga, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The epithelial cell composition was investigated in the follicle-associated intestinal crypt (FAIC) of rat Peyer's patches. The epithelium of the FAIC mainly consisted of columnar epithelial cells, goblet cells and Paneth cells. The characteristics of secretory granules in Paneth cells and goblet cells of both the FAIC and ordinary intestinal crypts (IC) were almost the same in periodic acid-Schiff (PAS) reaction, Alcian blue (AB) staining and the immunohistochemical detection of lysozymes and soluble phospholipase A2. Both goblet cells and Paneth cells were markedly less frequent on the follicular sides than on the anti-follicular sides of the FAIC. Goblet cells were also markedly less frequent in the follicle-associated epithelium (FAE) than in the ordinary intestinal villi (IV). Indigenous bacteria were more frequently adhered to FAE than to follicle-associated intestinal villi or IV. These findings suggest that the host defense against indigenous bacteria is inhibited on the follicular sides of FAIC, which might contribute to the preferential settlement of indigenous bacteria on the FAE; they also suggest that differentiation into secretory cells is inhibited in the epithelium of the follicular sides of FAIC, so that differentiation into M cells might be admitted in the FAE of rat Peyer's patches. Furthermore, intermediate cells possessing characteristics of both Paneth cells and goblet cells were rarely found in the FAIC, but not in the IC. This finding suggests that the manner of differentiation into Paneth cells in the FAIC differs from that in the IC.

    JAPAN SOC VET SCI, Jun. 2014, The Journal of veterinary medical science, 76 (6), 833 - 8, English, Domestic magazine

    [Refereed]

    Scientific journal

  • T. Omotehara, C. A. Smith, Y. Mantani, Y. Kobayashi, A. Tatsumi, D. Nagahara, R. Hashimoto, T. Hirano, Y. Umemura, T. Yokoyama, H. Kitagawa, N. Hoshi

    Sex of birds is genetically determined by the inheritance of sex chromosomes (ZZ for male and ZW for female), and the Z-linked gene named doublesex and mab-3 related transcription factor 1 (DMRT1) is a candidate sex- determining gene in avian species. However, the mechanisms underlying sex determination in birds are not yet understood, and the expression patterns of the DMRT1 protein in urogenital tissues have not been identified. In the current study, we used immunohistochemistry to investigate the detailed expression patterns of the DMRT1 protein in the urogenital systems (including Mullerian ducts) in male and female chicken embryos throughout embryonic development. Gonadal somatic cells in the male indifferent gonads showed stronger expressions of DMRT1 compared with those in the female indifferent gonads well before the presumptive period of the sex determination, and Sertoli cells forming testicular cords expressed DMRT1 in the testes after sex determination. Germ cells expressed DMRT1 equally in males and females after sex determination. The expression was continuous in males, but in females it gradually disappeared from the germ cells in the central part of the cortex of the left ovary toward both edges. The DMRT1 was also detected in the tubal ridge, which is a precursor of the Mullerian duct, and at the mesenchyme and outermost coelomic epithelium of the Mullerian duct in both sexes. Strong expression was observed in the males, but it was restricted to coelomic epithelium after the regression of the duct started. Thus, we observed the detailed spatiotemporal expression patterns of DMRT1 in the developing chicken urogenital systems throughout embryonic development, suggesting its various roles in the development of urogenital tissues in the chicken embryo.

    OXFORD UNIV PRESS, Apr. 2014, POULTRY SCIENCE, 93 (4), 953 - 958, English

    [Refereed]

    Scientific journal

  • Takashi Tanida, Ken Tasaka, Eiichi Akahoshi, Mitsuko Ishihara-Sugano, Michiko Saito, Shigehisa Kawata, Megumi Danjo, Junko Tokumoto, Youhei Mantani, Daichi Nagahara, Yoshiaki Tabuchi, Toshifumi Yokoyama, Hiroshi Kitagawa, Mitsuhiro Kawata, Nobuhiko Hoshi

    Fetal exposure to dioxins and related compounds is known to disrupt normal development of the midbrain dopaminergic system, which regulates behavior, cognition and emotion. The toxicity of these chemicals is mediated mainly by aryl hydrocarbon receptor (AhR) signaling. Previously, we identified a novel binding motif of AhR, the AhR-responsive element III (AHRE-III), in vitro. This motif is located upstream from the gene encoding tyrosine hydroxylase (TH), the rate-limiting enzyme of dopamine biosynthesis. To provide in vivo evidence, we investigated whether 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) could regulate AHRE-III transcriptional activity in midbrain dopaminergic neurons. We produced transgenic mice with inserted constructs of the AHRE-III enhancers, TH gene promoter and the c-myc-tagged luciferase gene. Single oral administrations of TCDD (0-2000 ng kg⁻¹ body weight) to the transgenic dams markedly enhanced TH-immunoreactive (ir) intensity in the A9, A10 and A8 areas of their offspring at 3 days and 8 weeks of age. The offspring of dams treated with 200 ng kg⁻¹ TCDD exhibited significant increases in the numbers of TH- and double (TH and c-myc)-ir neurons in area A9 compared with controls at 8 weeks. These results show that fetal exposure to TCDD upregulates TH expression and increases TH-ir neurons in the midbrain. Moreover, the results suggest that TCDD directly transactivates the TH promoter via the AhR-AHRE-III-mediated pathway in area A9. Fetal exposure to TCDD caused stable upregulation of TH via the AhR-AHRE-III signaling pathway and overgrowth of TH-ir neurons in the midbrain, implying possible involvement in the etiology of neurodevelopmental disorders such as attention-deficit/hyperactivity disorder (ADHD).

    WILEY, Feb. 2014, Journal of applied toxicology : JAT, 34 (2), 117 - 26, English, International magazine

    [Refereed]

    Scientific journal

  • Insight into the mechanism of reproductive dysfunction caused by neonicotinoid pesticides.

    Nobuhiko Hoshi, Tetsushi Hirano, Takuya Omotehara, Junko Tokumoto, Yuria Umemura, Youhei Mantani, Takashi Tanida, Katsuhiko Warita, Yoshiaki Tabuchi, Toshifumi Yokoyama, Hiroshi Kitagawa

    Neonicotinoids, which were developed in the 1990 s as an insecticide having selective toxicity, were later found to cause reproductive abnormalities in experimental animals. In Japan there is an attempt to preserve endangered animals, including the Japanese crested ibis, and there is a question of whether neonicotinoids affect the reproduction of this bird, since they are used in its habitat. Hence, we investigated whether the daily oral administration of the neonicotinoid clothianidin (CTD) has any deleterious effects on the reproductive function of mature male only or both young male and female quails as experimental animals. Vacuolization and the number of germ cells having fragmented DNA in seminiferous tubules, as well as the number and size of vacuoles in hepatocytes, increased dose-dependently. The ovaries showed abnormal histology in the granulosa cells, which produce progesterone. There were significant differences in egg-laying rates and embryo weights between the groups. Glutathione Peroxidase 4 (GPx4) and Manganese Superoxide Dismutase (Mn-SOD), which protect the organism from oxidative damage, showed a dose-dependent decrease. Thus, it is possible neonicotinoids affect the bird's reproductive system through oxidative stress, reflecting an imbalance between the production of reactive oxygen species (ROS) and a biological system's ability to readily detoxify the reactive intermediates or easily repair the resulting damage. Responding to our study, Sado Island has since succeeded in breeding Japanese crested ibis in the wild without the use of neonicotinoids.

    PHARMACEUTICAL SOC JAPAN, 2014, Biological & pharmaceutical bulletin, 37 (9), 1439 - 43, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Ultrastructural demonstration of the absorption and transportation of minute chylomicrons by subepithelial blood capillaries in rat jejunal villi.

    Ei-Ichirou Takahara, Youhei Mantani, Kankanam Gamage Sanath Udayanga, Wang-Mei Qi, Takashi Tanida, Takashi Takeuchi, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Chylomicrons from villous columnar epithelial cells are generally known to be transported only by central lymph vessels (CLV), whereas antigenic particulates derived from the intestinal lumen can also be transported by subepithelial blood capillaries (sBCs) in rat intestinal villi. The possibility of chylomicron absorption by sBCs was histoplanimetrically studied in the rat jejunum under a transmission electron microscope. The chylomicrons more abundantly presented in villous venules than in arterioles. The most frequent size (MFS) of chylomicrons was 75 to 90 nm in diameter in the areas near sBCs, while it was 45 to 60 nm in the epithelial intercellular spaces just above sBCs or the intermediate areas between sBCs. The MFS of chylomicrons was 45 to 60 nm in the intermediate areas between sBCs and in the epithelial intercellular spaces just above these areas. The MFS of chylomicrons in CLV was intermediate between that in the area adjacent to sBCs and that in the intermediate areas between sBCs. Chylomicrons were found in small vesicles in the endothelial cytoplasms of sBCs. No chylomicrons larger than 600 nm were observed in the lamina propria. These findings suggest that some of the chylomicrons smaller than 75 nm, which are probable intestinal very low-density lipoproteins (VLDL), are directly transported to the liver by hepatic portal blood in addition to CLV and that epithelial fat droplets larger than 600 nm are not discharged into lamina propria in rat jejunum under physiological conditions.

    THE JAPANESE SOCIETY OF VETERINARY SCIENCE, 30 Dec. 2013, The Journal of veterinary medical science, 75 (12), 1563 - 9, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Youhei Mantani, Ei-Ichirou Takahara, Takashi Takeuchi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The relationship between the invasion of indigenous bacteria into intestinal crypts and the proliferation of epithelial cells was histoplanimetrically investigated in the rat ascending colon. Indigenous bacteria preferentially adhered to the intestinal superficial epithelial cells in the mesenterium-attached mucosa (MAM) compared to those in the mesenterium-non-attached mucosa (MNM). Intestinal crypts with indigenous bacteria were also significantly more frequently found in MAM than in MNM. Total epithelial cells, columnar epithelial cells and goblet cells were significantly more abundant in the intestinal crypts with no-indigenous bacteria in MAM (MAM-C) than those in MNM (MNM-C), whereas the columnar epithelial cells were less abundant in MAM-C than in the intestinal crypts with indigenous bacteria in MAM (MAM-C-B). Columnar epithelial cells and goblet cells immuno-positive for proliferating cell nuclear antigen (PCNA) in MAM-C were more abundant than those in MNM-C, but less abundant than those in MAM-C-B. Toll-like receptor (TLR)-2, -4 and -9 were immuno-positive in the striated borders of the intestinal superficial epithelial cells, but their positive intensities were weaker in MAM than in MNM. From these findings, indigenous bacteria were confirmed to preferentially settle on the intestinal superficial epithelium of MAM in the rat ascending colon, and low TLRs-expression might contribute to the preferential settlement of indigenous bacteria in MAM. The increase of proliferating epithelial cells is probably induced by the invasion of indigenous bacteria into the intestinal crypts of MAM.

    31 Jul. 2013, The Journal of veterinary medical science, 75 (7), 939 - 47, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Histoplanimetrical study on the relationship between invasion of indigenous bacteria into intestinal crypts and proliferation of epithelial cells in rat ascending colon.

    MANTANI YOUHEI, TAKAHARA EI-ICHIRO, TAKEUCHI TAKASHI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    Jul. 2013, The Journal of Veterinary Medical Science, 75 (5), 939 - 947, English

    [Refereed]

    Scientific journal

  • Midori Yuji, Masato Fujimoto, Wang-Mei Qi, Ei-Ichrou Takahara, Youhei Mantani, Kankanam Gamage Sanath Udayanga, Takashi Takeuchi, Katsuhiko Warita, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Previously, the specific antibody-mediated persorption of antigenic molecules and particulates from the small-intestinal lumen into the peripheral blood was clarified in rats, but the intermediation of the receptor for the specific antibodies was not. In this study, the existence of receptor for the specific antibody was experimentally examined in the rat small intestine. Glutaraldehyde-fixed sheep erythrocytes (SEs) coated by Fc-fragments of IgG (IgG-Fc), (Fab')(2)-fragments of IgG (IgG-Fab) or bovine serum albumin (BSA), were injected into 3 jejunal loops each 2 cm in length in non-orally pre-immunized rats, respectively. Thirty minutes after the injection, IgG-Fc-coated SEs were significantly more engulfed by villous columnar epithelial cells than Fab- or BSA-coated SEs. The most frequent absorption sites were the intestinal villous apices. The IgG-Fc-coated SEs were adhered to the striated borders and were engulfed by villous columnar epithelial cells. IgG-Fc-coated SEs passing through the epithelial cells were also detected in the subepithelial blood capillaries just beneath the villous epithelium, but not in the connective tissue and the lymph vessels. These findings suggest that the absorption of luminal antigenic particulates is probably mediated by the Fc-receptor, and that the absorbed antigenic particulates are directly transferred to the hepatic portal blood by passing through the endothelium of the subepithelial blood capillaries.

    JAPAN SOC VET SCI, Nov. 2012, The Journal of veterinary medical science, 74 (11), 1447 - 52, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Youhei Mantani, Yuh Yokoo, Aosa Kamezaki, Kankanam Gamage Sanath Udayanga, Ei-ichirou Takahara, Takashi Takeuchi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Localization of Toll-like receptors (TLRs) in the exocrine glands associated with the rat alimentary tract was immunohistochemically studied using anti-TLR antibodies. TLR-2, -4 and -9 were detected in the secretory granules of acinar cells or the luminal substances of the gustatory gland, extraorbital lacrimal gland, Harderian gland, proper gastric gland and pancreas. TLR-2 and -9 were also detected in the mucous acinar cells of the sublingual gland. Positivity for all TLRs was found in the striated borders of columnar epithelial cells and the luminal substances of the intestinal crypts throughout the small intestine, and also in the goblet cells throughout the large intestine. Only TLR-4 was detected in the secretory granules of Paneth cells. A reduction of TLR-4-positive secretory granules and the formation of TLR-4-positive vacuoles were found in the ileal Paneth cells under the hyper-proliferation of indigenous bacteria. In the apical to middle intervillous portions of the ileum, Gram-positive bacterial colonies were significantly more abundant than Gram-negative bacterial colonies, whereas this difference disappeared in the basal intervillous portions. These findings suggest that there are distribution differences in the secretory sources of soluble TLRs that possibly neutralize their luminal ligands, in the rat alimentary tract. Therefore, the bacterial ligand-recognition system composed of the membranous TLRs of villous columnar epithelial cells and soluble TLRs from crypt epithelial cells might contribute to host defense mechanisms for the selective elimination of Gram-positive bacteria rather than Gram-negative bacteria in the rat small intestine.

    JAPAN SOC VET SCI, Nov. 2012, The Journal of veterinary medical science, 74 (11), 1429 - 38, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Kankanam Gamage Sanath Udayanga, Kyoji Yamamoto, Hidenori Miyata, Yuh Yokoo, Youhei Mantani, Ei-Ichrou Takahara, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    The apoptosis process in rat esophageal epithelium was investigated using enzyme-immunohistochemistry and transmission electron microscopy. As a result, Fas and Fas-L were expressed in the epithelial cell membrane and cytoplasm from the stratum spinosum (SS) to the stratum granulosum (SG). No TNF-R1 show immunopositivity in the cell membranes. TNF-α and caspase-8 were not observed in any layer. Caspase-10, cleaved caspase-3, XIAP and DNase-1 were found in the epithelial cytoplasm from the SS to the SG, whereas Bid, Apaf-1 and cleaved caspase-9 were detected only in the SG. Cytochrome c was observed as cytoplasmic granular positivity from the stratum basale (SB) and altered into homogeneous immunopositivity in the SG. Bcl-2 and Bcl-X immunopositivity was detected in cytoplasm from the SB to the SG. Immunoreactions of Bak in the cytoplasm and Bax beneath the cell membrane were observed from the upper portion of the SS with increasing intensity toward the SG. In the sites with the hyperproliferation of indigenous bacteria, TNF-R1, TNF-α and caspase-8 were detected in the SG and the immunopositive intensities of Bid, Apaf-1 and cleaved caspase-9 were altered to be strong. Prominently swollen cells and decreased mitochondria were ultrastructurally confirmed in the uppermost layers of stratum corneum. These findings suggest that the Fas-Fas-L-interaction initially induces apoptosis through a mitochondria-independent pathway and secondarily through a mitochondria-dependent pathway, leading to eventual epithelial cell death in the rat esophageal epithelium. The bacterial stimuli probably enhance the mitochondria-dependent pathway through the TNF-R1-TNF-α interaction.

    JAPAN SOC VET SCI, May 2012, The Journal of veterinary medical science, 74 (5), 597 - 605, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Site differences of Toll-like receptor expression in the mucous epithelium of rat small intestine

    Youhei Mantani, Aosa Kamezaki, Kankanam Gamage Sanath Udayanga, Ei-ichirou Takahara, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa

    Toll-like receptors (TLRs) are known to recognize pathogen-associated molecular patterns and might function as receptors to detect microbes. In this study, the distribution of TLR-2, -4 and -9 were immunohistochemically investigated in the rat small intestine. As a result, TLR-2 was detected in the striated borders of villous columnar epithelial cells throughout the small intestine, except for the apices of a small number of intestinal villi. TLR-4 and -9 were detected in the striated borders of the villous columnar epithelial cells only in the duodenum. TLR-4-immunopositive minute granules were found in the apical cytoplasms of epithelial cells, subepithelial spaces and blood capillary lumina. TLR-2 and -4 were detected in the striated borders of undifferentiated epithelial cells and in the luminal substances of the intestinal crypts throughout the small intestine, but TLR-9 was not detected in the crypts throughout the small intestine. Only TLR-4 was detected in the secretory granules of Paneth cells in both the jejunal and ileal intestinal crypts. These findings suggest that duodenal TLRs might monitor indigenous bacteria proliferation in the upper alimentary tract, that TLR-2 might also monitor the proliferation of colonized indigenous bacteria throughout the small intestine, that the lack of TLR-2 at the villous apices might contribute to the settlement of indigenous bacteria, and that TLR-2 and -4 are secreted from intestinal crypts.

    F HERNANDEZ, Oct. 2011, HISTOLOGY AND HISTOPATHOLOGY, 26 (10), 1295 - 1303, English

    [Refereed]

    Scientific journal

  • Immunohistochemical and histoplanimetrical study on the spatial relationship between the settlement of indigenous bacteria and the secretion of bactericidal peptides in rat alimentary tract.

    Yuh Yokoo, Hidenori Miyata, Kankanam Gamage Sanath Udayanga, Wang-Mei Qi, Ei-Ichirou Takahara, Youhei Mantani, Toshifumi Yokoyama, Junichi Kawano, Nobuhiko Hoshi, Hiroshi Kitagawa

    To clarify the regulatory mechanism by bactericidal peptides secretion, the secretion of bactericidal peptides was immunohistochemically and histoplanimetrically compared with the degree of Gram-positive/negative bacterial colonization throughout the rat alimentary tract. In the associated exocrine glands from the oral cavity to the stomach, no comparable differences were observed under the changes of development of indigenous bacterial colonies. In the small intestine, immunopositive granules for lysozyme and secretory phospholipase A2 (sPLA2) were markedly decreased, whereas immunopositive vacuoles in the Paneth cells were more increased at sites with hyper-development of indigenous bacterial colonies in the intervillous spaces than at sites with no or less development. No changes in exocrine glands were observed in the large intestine because of the constant existence of large quantities of bacteria. Gram-positive bacterial colonies on the mucosal surfaces were dominant from the oral cavity to the stomach. Gram-negative bacteria were dominant in the large intestine, and the distributions of both Gram-positive and negative bacteria were intermediate in the small intestine. These findings suggest that lysozyme and sPLA2 secreted from the Paneth cells contribute to the regulation of the proliferation of indigenous bacteria in the intervillous spaces of the small intestine, and that the inversion of distributions of Gram-positive and -negative bacteria in the alimentary tract might be caused by the secretion of lysozyme and sPLA2 in the small intestine.

    JAPAN SOC VET SCI, Aug. 2011, The Journal of veterinary medical science, 73 (8), 1043 - 50, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Immunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological condition

    K. G. S. Udayanga, H. Miyata, Y. Yokoo, W. -M. Qi, E. Takahara, Y. Mantani, T. Yokoyama, N. Hoshi, H. Kitagawa

    Epidermal homeostasis is maintained by both epithelial proliferation in the stratum basale (SB) and the apoptosis of epithelial cells under physiological conditions. In this study, the induction and regulation mechanisms of epidermal apoptosis were immunohistochemically investigated in the epidermis from Wistar rat's palm and foot pad by using several apoptotic related proteins under a physiological condition. The results showed that Fas and Fas-L were expressed in cellular membranes of the stratum spinosum (SS), whereas TNF-R1 did not show any membranous expression in any epidermal layers. TNF-alpha was not observed in the epidermis. Caspase-10, cleaved caspase-3 and DNase-1 were found in the epithelial cytoplasms from the SS to stratum granulosum (SG), whereas caspase-8 was not detected in the epidermis. XIAP and Bak were found in the cytoplasm from the SS to SG, and the intensity of Bak-positivity was stronger in the SG than the SS, whereas Bid, Apaf-1 and cleaved caspase-9 were restricted in the SG. Homogenous cytoplasmic immunoreactivity of Bcl-2 was found in the SB and the intensity was gradually decreased from the SB to the SG. The granular-cytoplasmic immuno-positivity of cytochrome C gradually altered into homogenous cytoplasmic expression in the upper half of the SG. Single-stranded DNA was rarely detected in the upper portion of the SG. These results suggest that epidermal apoptosis is induced by the interaction between Fas and Fas-L and the activation of caspase-10, and might initially proceed through a mitochondrial-independent pathway, and that a mitochondrial-dependent pathway finally accelerated under physiological conditions.

    F HERNANDEZ, Jul. 2011, HISTOLOGY AND HISTOPATHOLOGY, 26 (7), 811 - 820, English

    [Refereed]

    Scientific journal

MISC

  • 母子間移行における浸透性農薬およびその代謝産物の定量的な解明

    大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星信彦

    Dec. 2018, 日本内分泌かく乱化学物質学会研究発表会要旨集, 21st, 79, Japanese

  • 兵庫県における野生哺乳動物のネオニコチノイド系農薬への曝露実態調査

    杉田晄佑, 高田匡, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星信彦

    Dec. 2018, 日本内分泌かく乱化学物質学会研究発表会要旨集, 21st, 82, Japanese

  • 浸透性農薬とその代謝産物の胎子移行量の解明

    大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 杉田晄佑, 宮田結佳, 高田匡, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星信彦

    21 Aug. 2018, 日本獣医学会学術集会講演要旨集, 161st, 467, Japanese

  • ニワトリ胚におけるセルトリ細胞の起源(The origin of Sertoli cells in the chicken embryo)

    表原 拓也, 永堀 健太, 河田 晋一, 李 忠連, 宮宗 秀伸, 伊藤 正裕, 南 貴一, 万谷 洋平, 梅村 ゆりあ, 西田 美穂, 平野 哲史, 北川 浩, 横山 俊史, 星 信彦, 吉岡 秀文

    東京医科大学医学会, Jan. 2018, 東京医科大学雑誌, 76 (1), 139 - 140, English

  • ニワトリ胚におけるセルトリ細胞の起源(The origin of Sertoli cells in the chicken embryo)

    表原 拓也, 永堀 健太, 河田 晋一, 李 忠連, 宮宗 秀伸, 伊藤 正裕, 南 貴一, 万谷 洋平, 梅村 ゆりあ, 西田 美穂, 平野 哲史, 北川 浩, 横山 俊史, 星 信彦, 吉岡 秀文

    東京医科大学医学会, Jan. 2018, 東京医科大学雑誌, 76 (1), 139 - 140, English

Presentations

  • 性分化撹乱マウス系統(B6J-XYPOS)における性腺原基の卵巣化に関する免疫組織化学的研究

    中村大河,横山俊史,三浦由佳,加藤栞,奥西宣祐,成田大翔,万谷洋平,星信彦

    第96回日本解剖学会近畿支部学術集会, Nov. 2020, Japanese, Domestic conference

  • ラット消化管粘膜上皮表面における常在細菌の定着程度の概日リズムに関する定量組織学的解析

    坂田奈那美,万谷洋平,中西怜稀,横山俊史,星 信彦

    第96回日本解剖学会近畿支部学術集会, Nov. 2020, Japanese, Domestic conference

  • 無毒性量ネオニコチノイド系農薬がマウスの継世代雌性生殖器に及ぼす影響

    北内 佐也可,前田 瑞稀,西 美咲,正田 明日香,村田 碧,平野 哲史,万谷 洋平,横山 俊史,星 信彦

    第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference

  • 性分化撹乱マウス系統(B6J-XYPOS)における精巣化破綻機構に関する免疫組織化学的研究

    中村大河,横山俊史,三浦由佳,加藤栞,奥西宣祐,成田大翔,万谷洋平,星信彦

    第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference

  • ラット消化管各部位の粘膜上皮表面における常在細菌の定着程度の日内変動に関する組織学的解析

    坂田奈那美,万谷洋平,中西怜稀,横山俊史,星 信彦

    第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference

  • 腸管各部位の集合リンパ小節における細網細胞の部位差に関する免疫組織化学的研究

    土居萌花,万谷洋平,中西怜稀,坂田奈那美,横山俊史,星 信彦

    第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference

  • ラット大腸粘膜における線維芽細胞様細胞の部位差に関する組織学的研究

    万谷洋平, 田村彩貴, 大野伸彦, 横山俊史, 星 信彦.

    第163回日本獣医学会学術集会, Sep. 2020, Japanese, Domestic conference

  • 無毒性量ネオニコチノイド系農薬が次世代のマウス雌性生殖機能に及ぼす影響

    北内 佐也可,前田 瑞稀,平野 哲史,万谷 洋平,横山 俊史,星 信彦

    第60回日本先天異常学会学術集会, Jul. 2020, Japanese, Domestic conference

  • 無毒性量の浸透性農薬のマウス胎子・授乳期曝露による神経行動学的影響

    前田瑞稀,北内佐也加,平野哲史,万谷洋平,横山俊史,星 信彦

    第60回日本先天異常学会学術集会, Jul. 2020, Japanese, Domestic conference

  • ネオニコチノイド系農薬における野生哺乳動物の曝露状況と都道府県別の出荷量との関連

    杉田晄佑, 高田 匡, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • ネオニコチノイド系農薬クロチアニジンが次世代雌性生殖器に及ぼす影響

    北内佐也可, 前田瑞稀, 久保静花, 大成果乃子, 宮田結佳, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • クロチアニジンおよびその代謝産物の母子間移行における組織分布・蓄積性の定量的解明

    大野周嗣, 大成果乃子, 久保静花, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • 低用量クロチアニジンの胎子・授乳期曝露による神経行動学的影響

    前田瑞稀, 北内佐也可, 宮田結佳, 久保静花, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • 低用量クロチアニジンによる情動変容への加齢の影響評価

    宮田結佳, 久保静花, 大野周嗣, 大成果乃子, 前田瑞稀, 中西怜稀, 平野哲史, 万谷洋平, 横山俊史, 石塚真由美, 池中良徳, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • クロチアニジンの亜急性摂取はラット腸内細菌叢を変化させる

    大成果乃子, 大野周嗣, 久保静花, 中西怜稀, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • 低用量クロチアニジンによるマウスの物体認識・空間学習記憶障害とその性差

    久保静花, 宮田結佳, 大成果乃子, 大野周嗣, 平野哲史, 万谷洋平, 横山俊史, 星 信彦

    環境ホルモン学会第22回研究発表会, Japanese

    Poster presentation

  • マウス健常雄個体の性腺におけるSry 発現の左右差に関する定量組織学的研究

    川西 航平, 長谷川 千夏, 中村 大河, 加藤 栞, 万谷 洋平, 横山 俊史, 星 信彦

    第95回日本解剖学会近畿支部学術集会, Nov. 2019, Japanese, Domestic conference

    Oral presentation

  • Serial block-face 走査型電子顕微鏡を用いたラット回腸粘膜における神経接続の三次元解析

    中西 怜稀, 万谷 洋平, 春田 知洋, 横山 俊史, 星 信彦

    第95回日本解剖学会近畿支部学術集会, Nov. 2019, Japanese, Domestic conference

    Oral presentation

  • ラット小腸粘膜における好酸球の多様性に関する組織学的研究

    荒井真也, 万谷洋平, 中西怜稀, 春田知洋, 西田美穂, 湯浅秀人, 横山俊史, 星信彦, 北川浩

    第95回日本解剖学会近畿支部学術集会, Nov. 2019, Japanese, Domestic conference

    Oral presentation

  • GABA撹乱物質フィプロニルがマウスの情動行動に及ぼす影響

    前田 瑞稀, 大成 果乃子, 大野 周嗣, 久保 静花, 杉田 晄佑, 宮田 結佳, 北内 佐也加, 西 美咲, 高田 匡, 平野 哲史, 万谷 洋平, 横山 俊史, 池中 良徳, 星 信彦

    第162回日本獣医学会学術集会, Sep. 2019, Japanese, Domestic conference

    Oral presentation

  • 性決定期のマウス性腺におけるSry発現の時空間的解析-健常雄個体におけるSry発現の左右差

    川西 航平, 長谷川 千夏, 中村 大河, 加藤 栞, 万谷 洋平, 横山 俊史, 星 信彦

    第162回日本獣医学会学術集会, Sep. 2019, Japanese, Domestic conference

    Oral presentation

  • 性決定前のマウス未分化XY性腺の精巣化を誘導する器官培養法の確立とその組織学的評価

    長谷川 千夏, 梅村 ゆりあ, 川西 航平, 中村 大河, 加藤 栞, 万谷 洋平, 横山 俊史, 星 信彦

    第162回日本獣医学会学術集会, Sep. 2019, Japanese, Domestic conference

    Oral presentation

  • Serial block-face走査型電子顕微鏡(SBF-SEM)を用いたラット回腸の粘膜固有層における神経投射対象の解明

    中西怜稀, 万谷洋平, 春田知洋, 横山俊史, 星信彦

    第162回日本獣医学会学術集会, Sep. 2019, Japanese, Domestic conference

    Oral presentation

  • Serial block-face走査型電子顕微鏡を用いたラット回腸における単核食細胞系列の細胞と上皮内リンパ球の相互作用に関する研究

    万谷洋平, 春田知洋, 中西怜稀, 片田裕亮, 横山俊史, 星信彦, 北川浩

    第162回日本獣医学会学術集会, Sep. 2019, Japanese, Domestic conference

    Oral presentation

  • Three-Dimensional Observation of Mononuclear Phagocyte Lineages in the Rat Ileum using Serial Block-Face Scanning Electron Microscopy

    Youhei MANTANI, Tomohiro HARUTA, Satoki NAKANISHI, Yusuke KATADA, Toshifumi YOKOYAMA, Nobuhiko HOSHI, Hiroshi KITAGAWA

    19th International Congress of Mucosal Immunology, Jul. 2019, English, International conference

    Poster presentation

  • C57BL/6マウスの亜系統差による生殖腺表現型の変化

    三浦由佳, YOKOYAMA TOSHIFUMI, 山本杏, 川西航平, 長谷川千夏, 高田菜々子, MANTANI YOUHEI, HOSHI NOBUHIKO

    第32回モロシヌス研究会, Jun. 2019, Japanese, 千葉市, Domestic conference

    Oral presentation

  • Serial Block face走査型電子顕微鏡を用いたラット回腸粘膜における単核食細胞系列の細胞の三次元的観察

    MANTANI YOUHEI, 春田知洋, 中西怜稀, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第124回日本解剖学会総会全国学術集会, Mar. 2019, Japanese, 新潟市, Domestic conference

    Poster presentation

  • Adverse effects on cognitive-emotional behavior and immune system function in experimental animals administered a NOAEL-dose of neonicotinoids.

    HOSHI NOBUHIKO, YOSHINORI IKENAK, SHUJI OHNO, KANOKO ONARU, SHIZUKA KUBO, MIZUKI MAEDA, MIYATA YUKA, SUGITA KOUSUKE, MAYUMI ISHIZUKA, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI

    Chemical Hazard Symposium in Hokkaido, Mar. 2019, English, Sapporo, International conference

    [Invited]

    Nominated symposium

  • 母子間移行における浸透性農薬およびその代謝産物の定量的な解明

    大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, 石塚真由美, 池中良徳, HOSHI NOBUHIKO

    環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

    Poster presentation

  • 兵庫県における野生哺乳動物のネオニコチノイド系農薬への曝露実態調査

    杉田晄佑, 高田匡, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, 石塚真由美, 池中良徳, HOSHI NOBUHIKO

    環境ホルモン学会第21回研究発表会, Dec. 2018, Japanese, 東京都文京区, Domestic conference

    Poster presentation

  • 性決定遺伝子発現前のマウス未分化XY性腺の精巣化を誘導する器官培養法

    長谷川千夏, 梅村ゆりあ, 三浦由佳, 川西航平, 高田菜々子, 大野周嗣, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    関西実験動物研究会第140回研究会, Dec. 2018, Japanese, 京都市, Domestic conference

    Oral presentation

  • 性決定前のマウス未分化 XY 性腺の精巣化を誘導する器官培養法の確立とその組織学的評価

    長谷川千夏, 梅村ゆりあ, 三浦由佳, 川西航平, 高田菜々子, 大野周嗣, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第94回日本解剖学会近畿支部学術集会, Nov. 2018, Japanese, 神戸市, Domestic conference

    Oral presentation

  • ラット回腸粘膜における好酸球系列の細胞の組織学的研究

    荒井真也, MANTANI YOUHEI, 西田美穂, 湯浅秀人, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第94回日本解剖学会近畿支部学術集会, Nov. 2018, Japanese, 神戸市, Domestic conference

    Oral presentation

  • ラット回腸の上皮下線維芽細胞様細胞を制御し得る神経伝達物質の同定

    中西怜稀, MANTANI YOUHEI, 田村彩貴, 荒井真也, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第94回日本解剖学会近畿支部学術集会, Nov. 2018, Japanese, 神戸市, Domestic conference

    Oral presentation

  • 生殖腺の表現型に及ぼすマウス亜系統差の影響に関する遺伝学的研究

    三浦由佳, 山本杏, 川西航平, 長谷川千夏, 高田菜々子, 高田匡, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • 浸透性農薬の行動影響の性差

    久保静花, 宮田結佳, 大成果乃子, 大野周嗣, 杉田晄佑, 前田瑞稀, 高田匡, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • 浸透性農薬とその代謝産物の胎子移行量の解明

    大野周嗣, 大成果乃子, 久保静花, 坂田奈那美, 杉田晄佑, 宮田結佳, 高田匡, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, 石塚真由美, 池中良徳, HOSHI NOBUHIKO

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • ラット小腸粘膜におけるCD11b陽性細胞を中心とした各種免疫担当細胞の組織内配置に関する免疫組織化学的研究

    荒井真也, MANTANI YOUHEI, 西田美穂, 湯浅秀人, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • ラット呼吸器系粘膜におけるSP-AおよびSP-D分泌細胞の分布

    斉旺梅, 増田なつみ, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • ラット回腸腸絨毛における上皮下および非上皮下線維芽細胞様細胞の三次元的解析

    MANTANI YOUHEI, 春田知洋, 西田美穂, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • ラット回腸の上皮直下における線維芽細胞様細胞への神経投射に関する免疫組織化学的研究

    中西怜稀, MANTANI YOUHEI, 田村彩貴, 荒井真也, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬における哺乳動物への免疫毒性の検証

    大成果乃子, 大野周嗣, 久保静花, 中西怜稀, 荒井真也, 杉田晄佑, 宮田結佳, 高田匡, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬が老齢動物に及ぼす影響

    宮田結佳, 大成果乃子, 大野周嗣, 久保静花, 杉田晄佑, 高田匡, 前田瑞稀, 中西怜稀, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第161回日本獣医学会学術集会, Sep. 2018, Japanese, つくば市, Domestic conference

    Oral presentation

  • 発達期におけるネオニコチノイド系農薬曝露により引き起こされたドーパミン神経系の攪乱を伴う多動

    米田直起, 高田 匡, 大成果乃子, 大野周嗣, 久保静花, 杉田晄佑, 宮田結佳, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    環境ホルモン学会第20回研究発表会, Dec. 2017, Japanese, 神戸市, Domestic conference

    Poster presentation

  • 環境ストレスおよびネオニコチノイド系農薬ジノテフランの複合影響評価

    高田 匡, 米田直起, 大成果乃子, 大野周嗣, 久保静花, 杉田晄佑, 宮田結佳, 平野哲史, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    環境ホルモン学会第20回研究発表会, Dec. 2017, Japanese, 神戸市, Domestic conference

    Poster presentation

  • ラット大腸の上皮直下における間質細胞の部位差に関する免疫組織化学的研究

    田村彩貴, MANTANI YOUHEI, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第93回日本解剖学会近畿支部学術集会, Nov. 2017, Japanese, 大津市, Domestic conference

    Oral presentation

  • ラット回腸における間質細胞を介した細胞間ネットワーク解析へのSerial Block Face走査型電子顕微鏡の有用性

    MANTANI YOUHEI, 春田知洋, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第93回日本解剖学会近畿支部学術集会, Nov. 2017, Japanese, 大津市, Domestic conference

    Oral presentation

  • マウスのミュラー管退行におけるAMH作用様式

    山本杏, 表原拓也, 三浦由佳, 川西航平, 長谷川千夏, MANTANI YOUHEI, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第93回日本解剖学会近畿支部学術集会, Nov. 2017, Japanese, 大津市, Domestic conference

    Oral presentation

  • 生理的条件下のラット呼吸器系粘膜における補体成分C3と補体受容体C3aRおよびCD11bの局在に関する免疫組織化学的研究

    吉冨千晶, 増田なつみ, 湯浅秀人, 西田美穂, MANTANI YOUHEI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第160回日本獣医学会学術集会, Sep. 2017, Japanese, 鹿児島市, Domestic conference

    Oral presentation

  • ラットパイエル板におけるlysozyme陽性細胞を介する生体防御機構に関する免疫組織化学的研究

    宮本和樹, MANTANI YOUHEI, 湯浅秀人, 西田美穂, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第160回日本獣医学会学術集会, Sep. 2017, Japanese, 鹿児島市, Domestic conference

    Oral presentation

  • Serial block face-走査型電子顕微鏡を用いたラット回腸腸陰窩周囲の間質細胞に関する3次元超微形態学的解析

    MANTANI YOUHEI, 春田知洋, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第160回日本獣医学会学術集会, Sep. 2017, Japanese, 鹿児島市, Domestic conference

    Oral presentation

  • ニワトリの発生過程に特徴的な生殖腺の左右差

    表原拓也, 南貴一, MANTANI YOUHEI, 朝克吉楽, 梅村ゆりあ, 西田美穂, 平野哲史, 青山裕彦, 吉岡秀文, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第122回日本解剖学会総会・全国学術集会, Mar. 2017, Japanese, 長崎市, Domestic conference

    [Invited]

    Invited oral presentation

  • 胎子期および発達期におけるネオニコチノイド系農薬曝露がドーパミン神経系および行動に及ぼす影響

    米田直起, 平野哲史, 柳井翔吾, 高田匡, 岩本遥, 表原拓也, 片田裕亮, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

    Poster presentation

  • マウス胎子性腺における性分化関連遺伝子転写調節領域のDNAメチル化状態

    久保田直人, 表原拓也, 山本杏, 三浦由佳, 平野哲史, 南貴一, 柳井翔吾, 高田匡, 米田直起, 岩本遥, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第39回日本分子生物学会年会, Dec. 2016, Japanese, 横浜市, Domestic conference

    Poster presentation

  • ネオニコチノイド系農薬曝露によりみられた神経行動学的異常および標的脳領域の探索

    平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 南貴一, 久保田直人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬ジノテフラン曝露はうつ様行動を引き起こすか?

    高田匡, 平野哲史, 柳井翔吾, 米田直起, 岩本遥, 表原拓也, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬クロチアニジンは胎子型と成獣型,2種類のライディッヒ細胞にそれぞれ異なる影響を及ぼす

    柳井翔吾, 平野哲史, 表原拓也, 高田匡, 米田直起, 岩本遥, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第19回環境ホルモン学会研究発表会, Dec. 2016, Japanese, つくば市, Domestic conference

    Oral presentation

  • ラット呼吸器系粘膜を覆う粘液層における β-defensin 1 および β-defensin 2 の存在および分泌源の同定

    増田なつみ, MANTANI YOUHEI, 西田美穂, 湯浅秀人, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

    Oral presentation

  • ラット回腸粘膜における各種間質細胞の局在

    MANTANI YOUHEI, 宮本和樹, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

    Oral presentation

  • ラットパイエル板における濾胞被蓋上皮のアポトーシスとRANK/RANKLの発現との関係

    湯浅秀人, MANTANI YOUHEI, 増田なつみ, 西田美穂, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

    Oral presentation

  • マウス真性半陰陽個体のミュラー管退行とAMHの作用様式

    山本杏, 表原拓也, 久保田直人, 南貴一, 平野哲史, MANTANI YOUHEI, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

    Oral presentation

  • ニワトリ雌右性腺退縮メカニズムの解明

    南貴一, 表原拓也, 田渕圭章, 平野哲史, YOKOYAMA TOSHIFUMI, MANTANI YOUHEI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第92回日本解剖学会近畿支部学術集会, Nov. 2016, Japanese, 東大阪市, Domestic conference

    Oral presentation

  • 生理学的条件下のラットパイエル板におけるRANK/RANKLの発現および常在細菌の増殖による影響

    湯浅秀人, 増田なつみ, 西田美穂, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, MANTANI YOUHEI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • ラット呼吸器におけるβ-defensin 1およびβ-defensin 2を介した分泌型の生体防御機構に関する免疫組織化学的研究

    増田なつみ, MANTANI YOUHEI, 西田美穂, 湯浅秀人, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • ラット回腸におけるtelocyteの免疫組織化学的同定

    MANTANI YOUHEI, 宮本和樹, 西田美穂, 湯浅秀人, 増田なつみ, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • マウス真性半陰陽個体におけるAMHの作用様式に関する組織学的解析

    山本杏, 表原拓也, 久保田直人, 南貴一, 広川千英, 平野哲史, 磯江美智子, 柳井翔吾, 高田匡, 米田直起, 三浦由佳, 岩本遥, MANTANI YOUHEI, KITAGAWA HIROSHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬曝露とうつ発症との関連性の検証

    高田匡, 平野哲史, 柳井翔吾, 米田直起, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬ジノテフランによるドーパミン神経系の攪乱および多動性の誘発

    米田直起, 平野哲史, 柳井翔吾, 高田匡, 表原拓也, 久保田直人, 南貴一, 山本杏, 岩本遥, 三浦由佳, 磯江美智子, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬クロチアニジン急性投与が及ぼす行動学的影響とその作用点

    平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 久保田直人, 南貴一, 山本杏, 岩本遥, 三浦由佳, 磯江美智子, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第159回日本獣医学会学術集会, Sep. 2016, Japanese, 藤沢市, Domestic conference

    Oral presentation

  • 発達期におけるネオニコチノイド系農薬曝露により多動が引き起こされる

    米田直起, 平野哲史, 柳井翔吾, 高田匡, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 広川千英, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第56回 先天異常学会学術集会, Jul. 2016, Japanese, 姫路市, Domestic conference

    Poster presentation

  • 発達期におけるネオニコチノイド系農薬ジノテフラン曝露とうつ病発症との関係

    高田匡, 平野哲史, 柳井翔吾, 米田直起, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 広川千英, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第56回 先天異常学会学術集会, Jul. 2016, Japanese, 姫路市, Domestic conference

    Poster presentation

  • 胎子期から成獣期にかけてのネオニコチノイド系農薬クロチアニジン曝露が及ぼす行動学的影響

    平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 磯江美智子, 久保田直人, 南貴一, 広川千英, 山本杏, 岩本遥, 三浦由佳, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第56回 先天異常学会学術集会, Jul. 2016, Japanese, 姫路市, Domestic conference

    Poster presentation

  • ネオニコチノイド系農薬クロチアニジン単回投与によりみられた異常啼鳴および神経行動学的影響

    平野哲史, 柳井翔吾, 高田匡, 米田直起, 表原拓也, 久保田直人, 南貴一, 広川千英, 山本杏, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第43回日本毒性学会学術年会, Jun. 2016, Japanese, 名古屋市, Domestic conference

    Poster presentation

  • ニワトリ胚の性腺発生における左右差

    表原拓也, 南貴一, 万谷洋平, 梅村ゆりあ, 西田 美穂, 平野哲史, 吉岡 秀文, 北川浩, 横山俊史, 星信彦

    第121回日本解剖学会総会・全国学術集会, Mar. 2016, Japanese, 郡山市, Domestic conference

    Oral presentation

  • 環境ストレス下においてネオニコチノイド系農薬クロチアニジンが与える神経行動学的影響

    平野哲史, 柳井翔吾, 表原拓也, 高田匡, 米田直起, 梅村ゆりあ, 久保田直人, 南貴一, 広川千英, 山本杏, 万谷洋平, 横山俊史, 北川浩, 星信彦

    環境ホルモン学会第18回研究発表会, Dec. 2015, Japanese, 下野市, Domestic conference

    Poster presentation

  • ネオニコチノイド系農薬クロチアニジンの妊娠期及び授乳期曝露がマウス産子の雄性生殖器に与える影響

    柳井翔吾, 平野哲史, 表原拓也, 高田匡, 米田直起, 梅村ゆりあ, 久保田直人, 南貴一, 広川千英, 山本杏, 万谷洋平, 横山俊史, 北川浩, 星信彦

    環境ホルモン学会第18回研究発表会, Dec. 2015, Japanese, 下野市, Domestic conference

    Poster presentation

  • ニワトリ胚の左精巣に特異的なセルトリ細胞の起源

    表原拓也, 万谷洋平, 南貴一, 梅村ゆりあ, 平野哲史, 横山俊史, 北川浩, 星信彦

    第38回日本分子生物学会年会・第88回日本生化学会大会合同大会, Dec. 2015, Japanese, 神戸市, Domestic conference

    Oral presentation

  • 生理学的条件下におけるラットパイエル板濾胞被蓋上皮におけるToll-like receptor-4の発現とM細胞への分化との関係について

    湯浅秀人, 万谷洋平, 増田なつみ, 西田美穂, 河野潤一, 横山俊史, 星信彦, 北川浩

    第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

    Oral presentation

  • 性逆転系統(B6-XYPOS)マウスにおける性腺分化と遺伝的背景

    梅村ゆりあ, 表原拓也, 平野哲史, 久保田直人, 柳井翔吾, 増田なつみ, 湯浅秀人, 万谷洋平, 北川浩, 横山俊史, 星信彦

    第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

    Oral presentation

  • ラット呼吸器系粘膜を覆う液相に含まれるlysozymeおよびsPLA2の分泌源の特定

    増田なつみ, 万谷洋平, 西田美穂, 湯浅秀人, 河野潤一, 横山俊史, 星信彦, 北川浩

    第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

    Oral presentation

  • ラット回腸における間質細胞telocyteに関する超微形態学的研究

    万谷洋平, 西田美穂, 湯浅秀人, 増田なつみ, 横山俊史, 星信彦, 北川浩

    第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

    Oral presentation

  • ニワトリ胚の左性腺に特異的な細胞運命の追跡

    表原拓也, 南貴一, 万谷洋平, 梅村ゆりあ, 西田美穂, 平野哲史, 吉岡秀文, 横山俊史, 北川浩, 星信彦

    第91回日本解剖学会・近畿支部学術集会, Nov. 2015, Japanese, 京都市, Domestic conference

    Oral presentation

  • 環境ストレス下において浸透性農薬クロチアニジンが行動及び生殖機能に与える影響

    平野哲史, 柳井翔吾, 表原拓也, 梅村ゆりあ, 久保田直人, 南貴一, 高田匡, 米田直起, 広川千英, 山本杏, 万谷洋平, 横山俊史, 北川浩, 星信彦

    第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

    Oral presentation

  • ラット小腸のPaneth細胞に連絡する粘膜固有層細胞の超微形態

    万谷洋平, 西田美穂, 湯浅秀人, 増田なつみ, 横山俊史, 星信彦, 北川浩

    第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

    Oral presentation

  • ラット呼吸器系におけるlysozymeおよびsPLA2の分泌上皮細胞に関する免疫組織化学的研究

    増田なつみ, 万谷洋平, 西田美穂, 湯浅秀人, 河野潤一, 横山俊史, 星信彦, 北川浩

    第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

    Oral presentation

  • ラットパイエル板濾胞被蓋上皮におけるToll-like receptor-2, -4および-9の発現に対する常在細菌増殖の影響

    湯浅秀人, 万谷洋平, 増田なつみ, 西田美穂, 河野潤一, 横山俊史, 星信彦, 北川浩

    第158回日本獣医学会, Sep. 2015, Japanese, 十和田市, Domestic conference

    Oral presentation

  • ニワトリ胚における左精巣特異的な発生メカニズム

    表原拓也, 万谷洋平, 南貴一, 梅村ゆりあ, 平野哲史, 横山俊史, 北川浩, 星信彦

    日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 江別市, Domestic conference

    Oral presentation

  • The short-term effect of peptidoglycan injected into the intestinal lumen against host defense responses.

    MANTANI YOUHEI, MIYAMOTO KAZUKI, MATSUMORI SEIKO, YUASA HIDETO, MASUDA NATSUMI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    17th International Congress of Mucosal Immunology, Jul. 2015, English, Berlin, Germany, International conference

    Poster presentation

  • C57BL/6マウスの不可思議さ:その遺伝的背景に潜むSry-HMG box & Q-rich領域と性決定

    梅村ゆりあ, 橋本理恵, 表原拓也, 長原大知, 平野哲史, 久保田直人, 南貴一, 柳井翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第62回日本実験動物学会, May 2015, Japanese, 京都市, Domestic conference

    Poster presentation

  • Sex-related genes' asymmetric expression in early mouse gonads

    梅村 ゆりあ, 橋本 理恵, 表原 拓也, 平野 哲史, 長原 大知, 湯浅 秀人, 久保田 直人, 南 貴一, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第120回 日本解剖学会総会・全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

    Poster presentation

  • Selection of stable reference genes for quantitative RT-PCR analyses in developing mouse gonads

    YOKOYAMA TOSHIFUMI, 橋本 理恵, 梅村 ゆりあ, 表原 拓也, 平野 哲史, 長原 大知, 柳井 翔吾, 南 貴一, 久保田 直人, MANTANI YOUHEI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第120回 日本解剖学会総会・全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

    Poster presentation

  • Reproductive and behavioral effects of clothianidin in male mice in a chronically stressed condition

    HIRANO TETSUSI, YANAI SHOUGO, OMOTEHARA TAKUYA, HASHIMOTO RIE, UMEMURA YURIA, KUBOTA NAOTO, MINAMI KIICHI, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第120回 日本解剖学会総会・全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

    Poster presentation

  • Light and Electron Microscopical Study on the Particulate Bodies Found in Epithelium of Rat Airway.

    MANTANI YOUHEI, NISHIDA MIHO, YUASA HIDETO, MASUDA NATSUMI, TAKAHARA EIICHIROU, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第120回 日本解剖学会総会・全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

    Poster presentation

  • Left-right asymmetry of testicular formation in the chicken embryo

    表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, 久保田 直人, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第120回 日本解剖学会総会・全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

    Poster presentation

  • Differential Expression of Toll-like Receptor-2,-4 and -9 in the Various Type of Epithelia Associated with Rat Peyer’s Patches

    YUASA HIDETO, MANTANI YOUHEI, NISHIDA MIHO, TAKAHARA EI-ICHIROU, MASUDA NATSUMI, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第120回 日本解剖学会総会・全国学術集会, Mar. 2015, Japanese, 神戸市, Domestic conference

    Poster presentation

  • 消化管付属リンパ組織、パイエル板の上皮におけるToll-like receptor-2、-4および-9の発現に関する免疫組織化学的研究

    湯浅 秀人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    神戸大学研究基盤センター若手フロンティア研究会2014, Dec. 2014, Japanese, 神戸市, Domestic conference

    Poster presentation

  • 環境ストレス下動物の生殖機能抑制機序に関する形態機能学的研究

    平野 哲史, 柳井 翔吾, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    環境ホルモン学会第17回研究発表会, Dec. 2014, Japanese, 京都市, Domestic conference

    Oral presentation

  • 発生中のマウス胎子生殖腺における内部標準遺伝子の検討

    YOKOYAMA TOSHIFUMI, 橋本 理恵, 梅村 ゆりあ, 表原 拓也, 長原 大知, 平野 哲史, 久保田 直人, 南 貴一, 湯浅 秀人, 柳井 翔吾, MANTANI YOUHEI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • 通常飼育下のラットの呼吸器系に吸入された粒子状物の粘膜上皮への取り込みに関する組織学的研究

    MANTANI YOUHEI, 西田 美穂, 湯浅 秀人, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • ラット呼吸器系粘膜上皮におけるToll-like receptor -1-9の局在に関する網羅的解析

    池邊 正大, MANTANI YOUHEI, 西田 美穂, 湯浅 秀人, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • ラットパイエル板濾胞被蓋上皮および濾胞付属腸絨毛の上皮におけるToll-like receptor-2,-4および-9の発現特性

    湯浅 秀人, MANTANI YOUHEI, 西田 美穂, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • マウス胎子生殖腺における性分化関連遺伝子発現の左右差に関する分子形態学的研究

    橋本 理恵, 梅村 ゆりあ, 表原 拓也, 平野 哲史, 長原 大知, 湯浅 秀人, 久保田 直人, 南 貴一, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • ネオニコチノイド系農薬クロチアニジンの低用量曝露とストレスの複合影響

    平野 哲史, 表原 拓也, 橋本 理恵, 梅村 ゆりあ, 久保田 直人, 南 貴一, 柳井 翔吾, 長原 大知, 湯浅 秀人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • ニワトリ胚における性腺の形成機構とその左右差

    表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島市, Domestic conference

    Oral presentation

  • ニワトリ胚における性腺の形成メカニズムと左右差

    表原 拓也, 橋本 理恵, 梅村 ゆりあ, 平野 哲史, 南 貴一, 長原 大知, 湯浅 秀人, 久保田 直人, 柳井 翔吾, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第157回日本獣医学会学術集会, Sep. 2014, Japanese, 札幌市, Domestic conference

    Oral presentation

  • ラット上行結腸の集合リンパ小節近傍の腸陰窩に出現するパネート細胞様外分泌細胞に関する超微形態学的および組織化学的研究

    MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

    Oral presentation

  • ラット上行結腸における酸好性顆粒を有する腸陰窩上皮細胞に関する形態学的研究

    MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第89回日本解剖学会近畿支部学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

    Oral presentation

  • ラット空腸の腸絨毛における小型乳ビ球の門脈血への移行の仕組みに関する免疫組織化学的研究

    高原 英一郎, MANTANI YOUHEI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

    Oral presentation

  • ラットの消化器系における補体成分C3とC4の産生部位に関する免疫組織化学的研究

    又吉 緑, MANTANI YOUHEI, 高原 英一郎, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

    Oral presentation

  • ラット上行結腸腸陰窩における常在細菌の増殖に対する生体防御に関する研究

    MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

    Oral presentation

  • ラット十二指腸粘膜上皮におけるMyeloid differentiation factor-2とToll-like receptor-2の発現に関する免疫組織化学的研究

    樅山 真丈, MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

    Oral presentation

  • ラット空腸腸絨毛の毛細血管内皮における乳ビ球に対する受容体に関する免疫組織化学的研究

    高原 英一郎, MANTANI YOUHEI, 樅山真丈, 又吉 緑, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

    Oral presentation

  • ラット肝臓における補体C3およびC4の産生に関する免疫組織化学的研究

    又吉 緑, MANTANI YOUHEI, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第88回日本解剖学会近畿支部学術集会, Dec. 2012, Japanese, 神戸市, Domestic conference

    Oral presentation

  • ラット小腸腸陰窩上皮細胞の細胞死に関する超微形態学的および免疫組織化学的研究

    山本 教司, 樅山 真丈, UDAYANGA KANKANAM, GAMAGE SANATH, MANTANI YOUHEI, 高原 英一郎, 竹内 崇師, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

    Oral presentation

  • ラットの上行結腸における常在細菌の定着とその制御に関する定量組織学的研究

    MANTANI YOUHEI, 高原 英一郎, 竹内 崇師, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡市, Domestic conference

    Oral presentation

  • ラットの大腸粘膜上皮におけるToll-like receptorの発現と分泌

    MANTANI YOUHEI, 亀崎 青沙, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, 谷田 任司, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

    Oral presentation

  • ラットの呼吸器粘膜におけるToll-like receptorの発現と分泌

    亀崎 青沙, MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, 谷田 任司, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

    Oral presentation

  • Acceleration of Epithelial Apoptosis through the Secondary Activation of Mitochondria-Dependent Pathway in the Indigenous Bacteria Settlement Sites of Rat Tongue

    UDAYANGA KANKANAM, GAMAGE SANATH, YAMAMOTO KYOJI, MANTANI YOUHEI, TAKAHARA EI-ICHIRO, KAMEZAKI AOSA, TANIDA TAKASHI, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第87回日本解剖学会近畿支部学術集会, Dec. 2011, Japanese, 西宮市, Domestic conference

    Oral presentation

  • ラット大腸におけるToll-like receptorの局在に関する免疫組織化学的研究

    MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

    Oral presentation

  • ラット呼吸器系上皮におけるToll-like receptorの局在に関する免疫組織化学的研究

    亀崎 青沙, MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, 谷田 任司, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

    Oral presentation

  • Acceleration of epithelial apoptosis in anterior epithelial column of filiform papilla in rat tongue by indigenous bacterial settlement.

    UDAYANGA KANKANAM, GAMAGE SANATH, YAMAMOTO KYOUJI, MANTANI YOUHEI, TAKAHARA EI-ICHIRO, TANIDA TAKASHI, YOKOYAMA TOSHIFUMI, KAWANO JUNICHI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第152回日本獣医学会学術集会, Sep. 2011, Japanese, 堺市, Domestic conference

    Oral presentation

  • ラット消化管付属分泌腺からのToll-like receptorの分泌に関する免疫組織化学的研究

    MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第86回日本解剖学会近畿支部学術集会, Nov. 2010, Japanese, 泉佐野市, Domestic conference

    Oral presentation

  • Changes in apoptosis process of rat esophageal epithelium with hyperproliferation of the indigenous bacteria under a physiological condition

    UDAYANGA KANKANAM, GAMAGE SANATH, 山本教司, 宮田英典, 横尾 諭, MANTANI YOUHEI, 高原 英一郎, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第86回日本解剖学会近畿支部学術集会, Nov. 2010, Japanese, 泉佐野市, Domestic conference

    Oral presentation

  • ラット消化管附属分泌腺からのToll-like receptorの分泌に関する免疫組織化学的研究

    MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 高原 英一郎, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第150回日本獣医学会学術集会, Sep. 2010, Japanese, 帯広市, Domestic conference

    Oral presentation

  • ラット小腸腸絨毛におけるToll-like Receptorの発現

    MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 横尾 諭, 宮田英典, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第85回日本解剖学会近畿支部学術集会, Nov. 2009, Japanese, 橿原市, Domestic conference

    Oral presentation

  • 成獣雄マウスにおける性決定遺伝子Sry発現の分子学的解析

    藤井 裕香, YOKOYAMA TOSHIFUMI, 田阪 健, 須谷 真巳, MANTANI YOUHEI, 三觜 友子, 石原 可奈, 谷田 任司, KITAGAWA HIROSHI, HOSHI NOBUHIKO

    第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

    Oral presentation

  • ラット小腸の粘膜上皮におけるToll-like receptorの発現に関する免疫組織化学的研究

    MANTANI YOUHEI, UDAYANGA KANKANAM, GAMAGE SANATH, 横尾 諭, 宮田 英典, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    第148回日本獣医学会学術集会, Sep. 2009, Japanese, 鳥取市, Domestic conference

    Oral presentation

  • Immunohistochemical study on the Toll-like receptor expression in villous columnar epithelial cells of rat small intestine

    MANTANI YOUHEI, YOKOO YUH, UDAYANGA KANKANAM, GAMAGE SANATH, QI WANG-MEI, NAMBA MAKIKO, NISHIDA MIHO, KAWANO JUNICHI, YOKOYAMA TOSHIFUMI, HOSHI NOBUHIKO, KITAGAWA HIROSHI

    The 6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, 神戸市, International conference

    Poster presentation

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