Directory of Researchers

YAMAUCHI Yasuo
Graduate School of Agricultural Science / Department of Agrobioscience
Associate Professor
Agricultural Chemistry
Last Updated :2024/01/31

Researcher Profile and Settings

Affiliation

  • <Faculty / Graduate School / Others>

    Graduate School of Agricultural Science / Department of Agrobioscience
  • <Related Faculty / Graduate School / Others>

    Faculty of Agriculture / Department of Agrobioscience

Teaching

Research at Kobe

  • 11 May 2016, Why do tomatoes smell "grassy"? -Researchers identify enzymes that convert the grassy smell into a sweeter scent-

Research Activities

Research Areas

  • Life sciences / Plants: molecular biology and physiology

Awards

  • Dec. 2018 日本農芸化学会, 農芸化学技術賞, 作物の高温耐性を高める揮発性バイオスティミュラント「すずみどり」の開発

    YAMAUCHI YASUO

    Japan society

Published Papers

  • Kyohei Fukuda, Masayoshi Uefune, Hidehiro Fukaki, Yasuo Yamauchi, Ikuko Hara-Nishimura, Rika Ozawa, Kenji Matsui, Koichi Sugimoto, Kazunori Okada, Ryozo Imai, Kenshi Takahashi, Shinichi Enami, René Wurst, Junji Takabayashi

    One of the characteristic aspects of odour sensing in humans is the activation of olfactory receptors in a slightly different manner in response to different enantiomers. Here, we focused on whether plants showed enantiomer-specific response similar to that in humans. We exposed Arabidopsis seedlings to methanol (control) and (+)- or (−)-borneol, and found that only (+)-borneol reduced the root length. Furthermore, the root-tip width was more increased upon (+)-borneol exposure than upon (−)-borneol exposure. In addition, root-hair formation was observed near the root tip in response to (+)-borneol. Auxin signalling was strongly reduced in the root tip following exposure to (+)-borneol, but was detected following exposure to (−)-borneol and methanol. Similarly, in the root tip, the activity of cyclin B1:1 was detected on exposure to (−)-borneol and methanol, but not on exposure to (+)-borneol, indicating that (+)-borneol inhibits the meristematic activity in the root. These results partially explain the (+)-borneol-specific reduction in the root length of Arabidopsis. Our results indicate the presence of a sensing system specific for (+)-borneol in Arabidopsis.

    The Royal Society, May 2022, Biology Letters, 18 (5), English

    [Refereed]

    Scientific journal

  • Kyohei Fukuda, Masayoshi Uefune, Hidehiro Fukaki, Yasuo Yamauchi, Ikuko Nishimura, Rika Ozawa, Kenji Matsui, Koichi Sugimoto, Kazunori Okada, Ryozo Imai, René Wurst, Kenshi Takahashi, Shinichi Enami, Junji Takabayashi

    Apr. 2022, Biology Letters, English

    [Refereed]

    Scientific journal

  • Hiroshi Fukayama, Fumihiro Miyagawa, Naoki Shibatani, Aiko Koudou, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Yasuo Yamauchi, Daisuke Matsuoka, Ryutaro Morita

    CO2 -responsive CCT protein (CRCT) is a positive regulator of starch synthesis-related genes such as ADP-glucose pyrophosphorylase large subunit 1 and starch branching enzyme I particularly in the leaf sheath of rice (Oryza sativa L.). The promoter GUS analysis revealed that CRCT expressed exclusively in the vascular bundle, whereas starch synthesis-related genes were expressed in different sites such as mesophyll cell and starch storage parenchyma cell. However, the chromatin immunoprecipitation (ChIP) using a FLAG-CRCT overexpression line and subsequent qPCR analyses showed that the 5'-flanking regions of these starch synthesis-related genes tended to be enriched by ChIP, suggesting that CRCT can bind to the promoter regions of these genes. The monomer of CRCT is 34.2 kDa; however, CRCT was detected at 270 kDa via gel filtration chromatography, suggesting that CRCT forms a complex in vivo. Immunoprecipitation and subsequent MS analysis pulled down several 14-3-3-like proteins. A yeast two-hybrid analysis and bimolecular fluorescence complementation assays confirmed the interaction between CRCT and 14-3-3-like proteins. Although there is an inconsistency in the place of expression, this study provides important findings regarding the molecular function of CRCT to control the expression of key starch synthesis-related genes.

    Aug. 2021, Plant, cell & environment, 44 (8), 2480 - 2493, English, International magazine

    [Refereed]

    Scientific journal

  • Jun’ichi Mano, Sayaka Kanameda, Rika Kuramitsu, Nagisa Matsuura, Yasuo Yamauchi

    © 2019 Mano, Kanameda, Kuramitsu, Matsuura and Yamauchi. Oxidative stimuli to living cells results in the formation of lipid peroxides, from which various aldehydes and ketones (oxylipin carbonyls) are inevitably produced. Among the oxylipin carbonyls, those with an α,β-unsaturated bond are designated as reactive carbonyl species (RCS) because they have high electrophilicity and biological activity. Plants have arrays of dehydrogenases and reductases to metabolize a variety of RCS that occur in the cells, but these enzymes are not efficient to scavenge the most toxic RCS (i.e., acrolein) because they have only low affinity. Two glutathione transferase (GST) isozymes belonging to the plant-specific Tau class were recently observed to scavenge acrolein with KM values at a submillimolar level. This suggests that GST could also be involved in the defense system against RCS. We tested the activities of 23 Tau isozymes of Arabidopsis thaliana for five types of RCS, and the results revealed that 11 isozymes recognized either acrolein or 4-hydroxy-(E)-2-nonenal or both as a substrate(s). Such RCS-scavenging activities indicate the potential contribution of GST to RCS scavenging in plants, and they may account for the stress tolerance conferred by several Tau isozymes. RCS are therefore a strong candidate for endogenous substrates of plant GSTs.

    Apr. 2019, Frontiers in Plant Science, 10, English

    [Refereed]

    Scientific journal

  • Transcriptome analysis of Arabidopsis thaliana treated with green leaf volatiles: possible role of green leaf volatiles as self-made damage-associated molecular patterns

    YAMAUCHI YASUO, MATSUDA AYA, MATSUURA NAGISA, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    2018, Journal of Pesticide Science, English

    [Refereed]

    Scientific journal

  • Chemical Control of Abiotic Stress Tolerance Using Bioactive Compounds

    YAMAUCHI YASUO

    InTech, 2018, Plant, Abiotic Stress and Responses to Climate Change, English

    [Refereed]

    Scientific journal

  • Yoko Marutani, Yasuo Yamauchi, Mari Higashiyama, Akihito Miyoshi, Seiji Akimoto, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto

    Light and temperature affect state transitions through changes in the plastoquinone (PQ) redox state in photosynthetic organisms. We demonstrated that light and/or heat treatment induced preferential photosystem (PS) I excitation by binding light-harvesting complex II (LHCII) proteins. The photosystem of wheat was in state 1 after dark overnight treatment, wherein PQ was oxidized and most of LHCII was not bound to PSI. At the onset of the light treatment [25 A degrees C in the light (100 A mu mol photons m(-2) s(-1))], two major LHCIIs, Lhcb1 and Lhcb2 were phosphorylated, and the PSI-LHCII supercomplex formed within 5 min, which coincided with an increase in the PQ oxidation rate. Heat treatment at 40 A degrees C of light-adapted wheat led to further LHCII protein phosphorylation of, resultant cyclic electron flow promotion, which was accompanied by ultrafast excitation of PSI and structural changes of thylakoid membranes, thereby protecting PSII from heat damage. These results suggest that LHCIIs are required for the functionality of wheat plant PSI, as it keeps PQ oxidized by regulating photochemical electron flow, thereby helping acclimation to environmental changes.

    SPRINGER, Jan. 2017, PHOTOSYNTHESIS RESEARCH, 131 (1), 41 - 50, English

    [Refereed]

    Scientific journal

  • Mikiko Kunishima, Yasuo Yamauchi, Masaharu Mizutani, Masaki Kuse, Hirosato Takikawa, Yukihiro Sugimoto

    The green odor of plants is characterized by green leaf volatiles (GLVs) composed of C6 compounds. GLVs are biosynthesized from polyunsaturated fatty acids in thylakoid membranes by a series of enzymes. A representative member of GLVs (E)-2-hexenal, known as the leaf aldehyde, has been assumed to be produced by isomerization from (Z)-3-hexenal in the biosynthesis pathway; however, the enzyme has not yet been identified. In this study, we purified the (Z)-3:(E)-2-hexenal isomerase (HI) from paprika fruits and showed that various plant species have homologous HIs. Purified HI is a homotrimeric protein of 110 kDa composed of 35-kDa subunits and shows high activity at acidic and neutral pH values. Phylogenetic analysis showed that HIs belong to the cupin superfamily, and at least three catalytic amino acids (His, Lys, Tyr) are conserved in HIs of various plant species. Enzymatic isomerization of (Z)-3-hexenal in the presence of deuterium oxide resulted in the introduction of deuterium at the C4 position of (E)-2-hexenal, and a suicide substrate 3-hexyn-1-al inhibited HI irreversibly, suggesting that the catalytic mode of HI is a keto-enol tautomerism reaction mode mediated by a catalytic His residue. The gene expression of HIs in Solanaceae plants was enhanced in specific developmental stages and by wounding treatment. Transgenic tomato plants overexpressing paprika HI accumulated (E)-2-hexenal in contrast to wild-type tomato plants mainly accumulating (Z)-3-hexenal, suggesting that HI plays a key role in the production of (E)-2-hexenal in planta.

    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Jul. 2016, JOURNAL OF BIOLOGICAL CHEMISTRY, 291 (27), 14023 - 14033, English

    [Refereed]

    Scientific journal

  • Identification of (Z)-3:(E)-2-hexenal isomerases essential to the production of the leaf aldehydes in plants

    Mikiko Kunishima, Yasuo Yamauchi, Masaharu Mizutani, Masaki Kuse, Hirosato Takikawa, Yukihiro Sugimoto

    Jul. 2016, J. Biol. Chem., 291, 14023 - 14033, English

    [Refereed]

    Scientific journal

  • Yasuo Yamauchi, Mikiko Kunishima, Masaharu Mizutani, Yukihiro Sugimoto

    Abiotic stresses cause serious damage to plants; therefore, plants undergo a complicated stress response through signal transduction originating from environmental stimuli. Here we show that a subset of short-chain leaf volatiles with an alpha, beta-unsaturated carbonyl bond in their structure (reactive short-chain leaf volatiles, RSLVs) like (E)-2-hexenal and (E)-2-butenal can act as signal chemicals that strongly induce the gene expression of abiotic-related transcription factors, such as heat stress-related transcription factors (HSFA2, MBF1c) and other abiotic stress-related transcription factors (DREB2A, ZATs). RSLV-induced expression of HSFA2 and MBF1c was eliminated in HSFA1s-, known as heat stress response master regulators, knockout mutant, whereas those of DREB2A and ZATs were not, suggesting that the RSLV signaling pathway is composed of HSFA1-dependent and -independent pathways. RSLV treatment induced production of chaperon proteins, and the RSLV-treated Arabidopsis thus demonstrated enhanced abiotic stress tolerance. Because oxidative stress treatment enhanced RSLV production, we concluded that commonly found RSLVs produced by environmental stresses are powerful inducer of abiotic stress-related gene expression as oxidative stress signals.

    NATURE PUBLISHING GROUP, Jan. 2015, SCIENTIFIC REPORTS, 5, English

    [Refereed]

    Scientific journal

  • Yoko Marutani, Yasuo Yamauchi, Akihito Miyoshi, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto

    Photosystems of higher plants alleviate heat-induced damage in the presence of light under moderate stressed conditions; however, in the absence of light (i.e., in the dark), the same plants are damaged more easily. (Yamauchi and Kimura, 2011) We demonstrate that regulating photochemical energy transfer in heat-treated wheat at 40 degrees C with light contributed to heat tolerance of the photosystem. Chlorophyll fluorescence analysis using heat-stressed wheat seedlings in light showed increased non-photochemical quenching (NPQ) of chlorophyll fluorescence, which was due to thermal dissipation that was increased by state 1 to state 2 transition. Transmission electron microscopy revealed structural changes in thylakoid membranes, including unstacking of grana regions under heat stress in light. It was accompanied by the phosphorylation of thylakoid proteins such as D1 and D2 proteins and the light harvesting complex II proteins Lhcb1 and Lhcb2. These results suggest that heat stress at 40 degrees C in light induces state 1 to state 2 transition for the preferential excitation of photosystem I (PSI) by phosphorylating thylakoid proteins more strongly. Structural changes of thylakoid membrane also assist the remodeling of photosystems and regulation of energy distribution by transition toward state 2 probably contributes to plastoquione oxidation; thus, light-driven electrons flowing through PSI play a protective role against PSII damage under heat stress.

    MDPI AG, Dec. 2014, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 15 (12), 23042 - 23058, English

    [Refereed]

    Scientific journal

  • T. Inoue, Y. Yamauchi, A. H. Eltayeb, H. Samejima, A. G.T. Babiker, Y. Sugimoto

    The gas exchange of the upper fully expanded leaf of the root parasite Striga hermonthica and of its host Sorghum bicolor was measured under wet and dry conditions to identify the mechanisms of the devastating effects of the parasite on its hosts under drought. The short-term water stress severely reduced photosynthetic rate in infected sorghum, but less in S. hermonthica. Soil water stress did not affect leaf respiration rate in either S. hermonthica or infected sorghum. This suggests that under dry conditions both infected sorghum and S. hermonthica decreased autotrophic carbon gain. The transpiration rate of S. hermonthica, a major driving force for assimilate uptake from the host, was higher and less affected by water stress than that of infected sorghum. Stomatal density on the abaxial surfaces of the leaves was higher in S. hermonthica than in sorghum. Both S. hermonthica infection and water stress decreased stomatal conductance of the sorghum leaves. S. hermonthica, irrespective of soil water status, had greater stomatal aperture on the adaxial and abaxial surfaces of its leaves than infected sorghum. These results indicate that the higher transpiration rate of S. hermonthica even under water stress, achieved through higher stomatal density on the abaxial surfaces of the leaves and greater stomatal aperture on both surfaces of the leaves, may induce the maintenance of water and solute transfers from the host to the parasite leading to severe damage to the host under drought. © 2013 Springer Science+Business Media Dordrecht.

    Dec. 2013, Biologia Plantarum, 57 (4), 773 - 777, English

    [Refereed]

    Scientific journal

  • Photosynthetic capacity and stomatal response of root hemi-parasite Striga hermonthica and sorghum under short-term soil water stress

    Inoue, T, YAMAUCHI YASUO, Amani, AE, Samejima, H, Abdel Gabar, EB, Sugimoto, Y

    2013, Biologia Plantarum, English

    [Refereed]

    Scientific journal

  • Marutani Yoko, Yamauchi Yasuo, Kimura Yukihiro, Mizutani Masaharu, Sugimoto Yukihiro

    Aug. 2012, PLANTA, 236 (2), 753 - 761

    [Refereed]

  • Atsushi Nakai, Yasuo Yamauchi, Sawako Sumi, Kiyoshi Tanaka

    Acylamino acid-releasing enzyme/oxidized protein hydrolase (AARE/OPH) has been biochemically demonstrated to be a bifunctional protease that has exopeptidase activity against N-alpha-acylated peptides and endopeptidase activity against oxidized and glycated proteins; however, its physiological role remains unknown. In this study, to determine its physiological significance, we produced AARE/OPH-overexpressing and -suppressed plants and assessed the biological impacts of AARE/OPH. The subcellular localization of Arabidopsis AARE/OPH was found to be cytoplasmic and nuclear by transient expression analysis of tdTomato-fused Arabidopsis AARE/OPH. Overexpression of AARE/OPH exhibited no apparent effect on the level of oxidized proteins because wild types probably have inherently high AARE/OPH activity. Through RNAi gene suppressing, we successfully produced AARE/OPH-suppressed Arabidopsis plants (aare) that exhibited almost no AARE activity. In the aare plant, electrolyte leakage by methyl viologen treatment was enhanced compared to that of non-transformant plants, suggesting that the plasma membranes of aare easily suffered oxidative damage, probably as a result of deterioration of the cytoplasmic antioxidative system. Correspondingly, proteomic analysis revealed that the aare plant accumulated a number of oxidized proteins including cytoplasmic antioxidant enzymes. On the basis of these results, we concluded that AARE/OPH plays a homeostatic role in sustaining the cytoplasmic antioxidative system.

    SPRINGER, Aug. 2012, PLANTA, 236 (2), 427 - 436, English

    [Refereed]

    Scientific journal

  • Yasuo Yamauchi, Ayaka Hasegawa, Masaharu Mizutani, Yukihiro Sugimoto

    Lipid peroxide-derived reactive carbonyls (RCs) can cause serious damage to plant functions. A chloroplastic NADPH-dependent alkenal/one oxidoreductase (AOR) detoxifies RCs, but its physiological significance remains unknown. In this study, we investigated the biological impacts of AOR using an AOR-knock out Arabidopsis line (aor). Methyl viologen treatment, mainly to enhance photosystem (PS) I-originated reactive oxygen species (ROS) production, caused more severe damage to aor than wild type (Col-0). In contrast, the high light treatment used to enhance PSII-originated ROS production resulted in no difference in PSII damage between Col-0 and aor. In conclusion, AOR can contribute to detoxify stromal RCs produced under oxidative stress. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

    ELSEVIER SCIENCE BV, Apr. 2012, FEBS LETTERS, 586 (8), 1208 - 1213, English

    [Refereed]

    Scientific journal

  • Germination response of Striga hermonthica and Orobanche minor seeds pre-treated with the synthetic strigolactone GR24

    Sawada, R, YAMAUCHI YASUO, Sugimoto, Y

    2012, Recent Research Developments in Phytochemistry, 10, 1 - 12, English

    [Refereed]

    Scientific journal

  • Function of extrinsic proteins on stabilization of photosynthetic oxygen-evolving complex

    Yong, L, Kimura, Y, Ohno, T, YAMAUCHI YASUO

    2012, Molecular Photochemistry, 6, 109 - 124, English

    [Refereed]

    Scientific journal

  • Yasuo Yamauchi, Ayaka Hasegawa, Ai Taninaka, Masaharu Mizutani, Yukihiro Sugimoto

    Reactive carbonyls, especially alpha,beta-unsaturated carbonyls produced through lipid peroxidation, damage biomolecules such as proteins and nucleotides; elimination of these carbonyls is therefore essential for maintaining cellular homeostasis. In this study, we focused on an NADPH-dependent detoxification of reactive carbonyls in plants and explored the enzyme system involved in this detoxification process. Using acrolein (CH(2) = CHCHO) as a model alpha,beta-unsaturated carbonyl, we purified a predominant NADPH-dependent acrolein-reducing enzyme from cucumber leaves, and we identified the enzyme as an alkenal/one oxidoreductase (AOR) catalyzing reduction of an alpha,beta-unsaturated bond. Cloning of cDNA encoding AORs revealed that cucumber contains two distinct AORs, chloroplastic AOR and cytosolic AOR. Homologs of cucumber AORs were found among various plant species, including Arabidopsis, and we confirmed that a homolog of Arabidopsis (At1g23740) also had AOR activity. Phylogenetic analysis showed that these AORs belong to a novel class of AORs. They preferentially reduced alpha,beta-unsaturated ketones rather than alpha,beta-unsaturated aldehydes. Furthermore, we selected candidates of other classes of enzymes involved in NADPH-dependent reduction of carbonyls based on the bioinformatic information, and we found that an aldo-keto reductase (At2g37770) and aldehyde reductases (At1g54870 and At3g04000) were implicated in the reduction of an aldehyde group of saturated aldehydes and methylglyoxal as well as alpha,beta-unsaturated aldehydes in chloroplasts. These results suggest that different classes of NADPH-dependent reductases cooperatively contribute to the detoxification of reactive carbonyls.

    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Mar. 2011, JOURNAL OF BIOLOGICAL CHEMISTRY, 286 (9), 6999 - 7009, English

    [Refereed]

    Scientific journal

  • Plants switch photosystem at high temperature to protect photosystem II

    YAMAUCHI YASUO, Marutani, Y, Akimoto, S, Kimura, Y, Mizutani, M, Sugimoto, Y

    2011, Nature Procedings, 6168, English

    Scientific journal

  • Photosystem at high temperature: Mechanisms of adaptation and damage. Photochemistry: UV/VIS Spectroscopy

    YAMAUCHI YASUO, Kimura, Y

    2011, UV/VIS Spectroscopy, Photochemical Reactions and Photosynthesis, 203 - 236, English

    [Refereed]

    Scientific journal

  • Yasuo Yamauchi, Yukihiro Sugimoto

    Previously we observed that the oxygen-evolving complex 33 kDa protein (OEC33) which stabilizes the Mn cluster in photosystem II (PSII), was modified with malondialdehyde (MDA), an end-product of peroxidized polyunsaturated fatty acids, and the modification increased in heat-stressed plants (Yamauchi et al. 2008). In this study, we examined whether the modification of OEC33 with MDA affects its binding to the PSII complex and causes inactivation of the oxygen-evolving complex. Purified OEC33 and PSII membranes that had been removed of extrinsic proteins of the oxygen-evolving complex (PSIIa dagger OEE) of spinach (Spinacia oleracea) were separately treated with MDA. The binding was diminished when both OEC33 and PSIIa dagger OEE were modified, but when only OEC33 or PSIIa dagger OEE was treated, the binding was not impaired. In the experiment using thylakoid membranes, release of OEC33 from PSII and corresponding loss of oxygen-evolving activity were observed when thylakoid membranes were treated with MDA at 40A degrees C but not at 25A degrees C. In spinach leaves treated at 40A degrees C under light, maximal efficiency of PSII photochemistry (F (v)/F (m) ratio of chlorophyll fluorescence) and oxygen-evolving activity decreased. Simultaneously, MDA contents in heat-stressed leaves increased, and OEC33 and PSII core proteins including 47 and 43 kDa chlorophyll-binding proteins were modified with MDA. In contrast, these changes were to a lesser extent at 40A degrees C in the dark. These results suggest that MDA modification of PSII proteins causes release of OEC33 from PSII and it is promoted in heat and oxidative conditions.

    SPRINGER, Apr. 2010, PLANTA, 231 (5), 1077 - 1088, English

    [Refereed]

    Scientific journal

  • Rieko Hori, Gen Sugimoto, Miharu Matsui, Yasuo Yamauchi, Hirosato Takikawa, Yukihiro Sugimoto

    Dechlorodauricumine (5) and dechloroacutumine (6) were converted to miharumine (7) and dechloroacutumidine (8), respectively, by a cell-free preparation from cultured roots of Menispermum dauricum in the presence of FAD. The structures of 7 and 8 were elucidated on the basis of spectroscopic analyses and chemical conversion.

    TAYLOR & FRANCIS LTD, Feb. 2009, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73 (2), 440 - 442, English

    [Refereed]

    Scientific journal

  • Yasuo Yamauchi, Ai Furutera, Kumiko Seki, Yasuyuki Toyoda, Kiyoshi Tanaka, Yukihiro Sugimoto

    When polyunsaturated fatty acids (PUFAs) in biomembrane are peroxidized, a great diversity of aldehydes is formed, and some of which are highly reactive. Thus they are thought to have biological impacts in stressed plants; however, the detailed mechanism of generation and biochemical effects are unknown. In this study, we show that chloroplasts are major organelles in which malondialdehyde (MDA) generated from peroxidized linolenic acid modifies proteins in heat-stressed plants. First, to clarify the biochemical process of MDA generation from PUFAs and its attachment to proteins, we carried out in vitro experiments using model proteins (BSA and Rubisco) and methylesters of C18 PUFAs that are major components of plant biomembrane. Protein modification was detected by Western blotting using monoclonal antibodies that recognize MDA binding to proteins. Results showed that peroxidation of linolenic acid methylester by reactive oxygen species was essential for protein modification by MDA, and the MDA modification was highly dependent on temperature, leading to a loss of Rubisco activity. When isolated spinach thylakoid membrane was peroxidized at 37 degrees C, oxygen-evolving complex 33 kDa protein (OEC33) was modified by MDA. These model experiments suggest that protein modification by MDA preferentially occurs under higher temperatures and oxidative conditions, thus we examined protein modification in heat-stressed plants. Spinach plants were heat-stressed at 40 degrees C under illumination, and modification of OEC33 protein by MDA was detected. In heat-stressed Arabidopsis plants, light-harvesting complex protein was modified by MDA under illumination. This modification was not observed in linolenic acid-deficient mutants (fad3fad7fad8 triple mutant), suggesting that linolenic acid is a major source of protein modification by MDA in heat-stressed plants. (C) 2008 Elsevier Masson SAS. All rights reserved.

    ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, Aug. 2008, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 46 (8-9), 786 - 793, English

    [Refereed]

    Scientific journal

  • Marker assisted transfer of stay-green trait of sorghum to enhance terminal drought tolerance: Candidate donor and recipient genotypes

    Abdelbagi, MA, Nasrein, M, Ibrahim, N, Hiraoka, Y, YAMAUCHI YASUO, Sugimoto, Y

    2007, Sudan Journal of Agricultural Research, 10, 133 - 141, English

    [Refereed]

    Scientific journal

  • Development of methods for galactinol production using sucrose as a starting material.

    Yasuo Yamauchi

    2006, Proceedings of the Research Society of Japan Suger Refineriesユ Technologists, 54, 37-43, English

    Scientific journal

  • YH Qi, N Kawano, Y Yamauchi, JQ Ling, DB Li, K Tanaka

    A submergence-induced gene, OsGGT, was cloned from 7-day submerged rice (Oryza sativa L. plants, FR13A (a submergence-tolerant cultivar, Indica), using suppression subtractive hybridization and both 5'- and 3'-rapid amplification of cDNA ends (RACE). The full-length OsGGT cDNA contains 1,273 bp with an open reading frame of 1,140 bp (17-1,156) that encodes 379 amino acids. Its deduced amino acid sequence is homologous with glycogenin glucosyltransferase. We found that the OsGGT gene is located in the 17,970-20,077 bp region of genome fragment AAAA01002475.1 of the Indica cultivar and in the 53,293-51,186 bp region of genome fragment AC037426.12 of chromosome 10 of the Japanica cultivar. A time-course study showed that OsGGT-gene expression increased in FR13A during submergence but decreased in IR42 (submergence-intolerant cultivar, Indica). The expression of the OsGGT gene in FR13A was induced by salicylic acid and benzyladenine. The accumulation of OsGGT mRNA in FR13A also increased in response to ethylene, gibberellin, abscisic acid, drought and salt treatment, but methyl jasmonate treatment and cold stress had no effect on expression. These results suggest that the OsGGT gene could be related to submergence stress and associated with a general defensive response to various environmental stresses.

    SPRINGER, Jun. 2005, PLANTA, 221 (3), 437 - 445, English

    Scientific journal

  • YH Qi, Y Yamauchi, JQ Ling, N Kawano, DB Li, K Tanaka

    We used suppression subtractive hybridization (SSH) to identify submergence-induced genes from the submergence-tolerant rice cultivar, FR13A. These genes putatively encode four proteins including a cation transport protein (OsCTP), monogalactosyl-diacylglycerol synthase, a cold-induced protein and glutathione synthetase. The full-length gene of one upregulated clone, OsCTP was generated by rapid amplification of cDNA ends. The gene comprises 741 bp with an open reading frame of 414 bp (nt 51-464) that encodes a putative protein of 137 amino acids. The theoretical isoelectric point of the putative protein is 5.35 and the molecular weight is 15.2 kDa. Six exons and five introns of the OsCTP gene correspond to 3582-2296 bp of accession number AAAA01013244 from the rice genome. OsCTP expression is enhanced by submergence as well as stress induced by abscisic acid, salt or drought. OsCTP might encode a novel cation transport protein similar to Escherichia coli ChaC and may be associated with a general defensive response to various environmental stresses. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

    ELSEVIER SCI IRELAND LTD, Jan. 2005, PLANT SCIENCE, 168 (1), 15 - 22, English

    Scientific journal

  • YH Qi, Y Yamauchi, JQ Ling, N Kawano, DB Li, K Tanaka

    Suppression subtractive hybridization was used to construct a subtractive cDNA library from plants of non-submerged and 7-day-submerged rice (Oryza sativa L., FR13A, a submergence-tolerant cultivar). One clone of the subtractive cDNA library, S23, was expressed abundantly during submergence. The full length of S23 was amplified using 5'- and 3'-rapid amplification of cDNA ends, and found to consist of 1,671 bp with an open reading frame of 1,077 bp (181-1257) encoding 358 amino acids. Its deduced amino acid sequence showed a high homology with monogalactosyldiacylglycerol synthase (UDPgalactose: 1,2-diacylglycerol 3-beta-D-galactosyl transferase; EC 2.4.1.46, MGDG synthase) from Arabidopsis thaliana; therefore, we named the gene OsMGD. Time-course studies showed that the expression of OsMGD in the rice cultivars FR13A and IR42 (submergence-susceptive cultivar) during submergence was gradually increased and that expression in FR13A was higher than in IR42. The expression of OsMGD in FR13A was influenced by benzyladenine and illumination. The accumulation of OsMGD mRNA in both FR13A and IR42 was also increased by ethephon, gibberellin, drought and salt treatment, but cold stress had no effect on the expression of the gene. These results suggest that the expression of OsMGD mRNA requires benzyladenine or illumination, and that the process is also mediated by ethephon and gibberellin. Salt and drought stress have an effect similar to that of submergence. Furthermore, the enhanced expression of OsMGD may relate to photosynthesis, and play an important role during submergence.

    SPRINGER, Jul. 2004, PLANTA, 219 (3), 450 - 458, English

    Scientific journal

  • GH Badawi, N Kawano, Y Yamauchi, E Shimada, R Sasaki, A Kubo, K Tanaka

    The role of APX (ascorbate peroxidase) in protection against oxidative stress was examined using transgenic tobacco plants. The full length cDNA, coding Arabidopsis thaliana L. APX fused downstream to the chloroplast transit sequence from A. thaliana glutathione reductase, was cloned into appropriate binary vector and mobilized into Agrobacterium tumefaciens C58C2. Leaf discs were infected with the Agrobacterium and cultured on medium supplied with kanamycin. The incorporation of the gene in tobacco genome was confirmed by Southern dot blot hybridization. Transgenic lines were generated, and the line Chl-APX5 shown to have 3.8-fold the level of APX activity in the wild-type plants. The isolated chloroplasts from this line showed higher APX activity. During early investigation, this line showed enhanced tolerance to the active oxygen-generating paraquat and sodium sulphite. The first generation of this line, also, showed enhanced tolerance to salt, PEG and water stresses, as determined by net photosynthesis. The present data indicate that overproducing the cytosolic APX in tobacco chloroplasts reduces the toxicity of H(2)O(2).

    WILEY-BLACKWELL, Jun. 2004, PHYSIOLOGIA PLANTARUM, 121 (2), 231 - 238, English

    Scientific journal

  • Enhanced tolerance to water deficit and high salt stress by overexpressing superoxide dismutase and ascorbate peroxidase in tobacco chloroplasts

    GH Badawi, Y Yamauchi, N Kawano, K Tanaka, K Tanaka

    OXFORD UNIV PRESS, 2004, PLANT AND CELL PHYSIOLOGY, 45, S230 - S230, English

    Scientific journal

  • Y Yamauchi, Y Ejiri, Y Toyoda, K Tanaka

    Plant acylamino acid-releasing enzyme (AARE) catalyzing the N-terminal hydrolysis of N-alpha-acylpeptides to release N-alpha-acylated amino acids, was biochemically characterized using recombinant and native AAREs. A cDNA encoding a deduced Arabidopsis thaliana AARE (AtAARE) was cloned and sequenced. The deduced amino acid sequence encoded a 764 amino acid protein of 83.9 kDa, which was 31.8% identical with that of rat AARE. In particular, the proposed catalytic residues (Ser, Asp, and His) of AARE, called the "catalytic triad residues," were completely conserved. Recombinant AtAARE was expressed in Escherichia coli and confirmed to be a functional AARE. Native AAREs were prepared from A. thaliana and cucumber (Cucumis sativus, L.) plants. Both native AAREs were tetrameric proteins of 350 kDa comprising four subunits of 82 kDa, and showed typical enzymological properties of other AAREs, i.e. sensitivity to diisopropyl fluorophosphate, an optimum pH of around 7.0, and an optimum temperature of 37degreesC. Both the native and recombinant AAREs were immunochemically homologous. Intracelluar fractionation analysis showed that the AARE was mainly present in the stroma of chloroplasts. Native AARE degraded the glycated ribulose-1,5-bisphoshate carboxylase/oxygenase protein but not the native protein. Thus, plant AARE might be involved in not only catalysis of the N-terminal hydrolysis of N-alpha-acylpeptides but also the elimination of glycated proteins.

    JAPANESE BIOCHEMICAL SOC, Aug. 2003, JOURNAL OF BIOCHEMISTRY, 134 (2), 251 - 257, English

    Scientific journal

  • ES Ella, N Kawano, Y Yamauchi, K Tanaka, AM Ismail

    Large areas of rainfed lowlands in South and Southeast Asia annually experience short durations of flash flooding during the rice-growing season, which dramatically affect plant survival and productivity. Submergence-intolerant cultivars usually show progressive leaf chlorosis, which could be triggered by ethylene produced during submergence. An ethylene inhibitor, 1-methyl cyclopropene (MCP), was used to evaluate the effect of ethylene on chlorophyll degradation and plant survival. Seedlings of two cultivars, FR13A (tolerant) and IR42 (intolerant) either untreated or treated with MCP for 5 h before submergence, were submerged for 6 d. Chlorophyll content and activity and gene expression of chlorophyllase, the first enzyme involved in chlorophyll degradation, were determined during submergence and recovery, and survival scores were recorded after 21 d of recovery. MCP treatment decreased chlorophyll degradation, lowered the activity and gene expression of chlorophyllase, and improved seedling survival in IR42, but with no effect on underwater relative shoot expansion. Chlorophyllase enzyme activity and gene expression were lower in FR13A, suggesting that this cultivar might have reduced ethylene level or sensitivity. Manipulation of ethylene synthesis or sensitivity through molecular approaches might therefore help improve tolerance of flash flooding in rice.

    C S I R O PUBLISHING, 2003, FUNCTIONAL PLANT BIOLOGY, 30 (7), 813 - 819, English

    Scientific journal

  • YAMAUCHI Yasuo, EJIRI Yukinori, TANAKA Kiyoshi

    In this study, a highly active foliar aminopeptidase preferentially releasing N-terminal alanine from artificial substrates was purified and characterized from cucumber (Cucumis sativus L. suyo). The enzyme had a molecular mass of 200 kDa consisting of two subunits of 95 kDa. It was a metalloprotease the pH optimum of which was 8 to 9. It cleaved Ala-, Gly-, Met-, Ser-, Leu-, Lys-, and Arg artificial substrates. An internal amino acid sequence was similar to those of aminopeptidase N (clan MA, family Ml) of microorganisms, and was very similar to that of a putative aminopeptidase N of Arabidopsis thaliana. From these results, the highly active aminopeptidase in cucumber leaves was identified to be a plant aminopepitdase N.

    Japan Society for Bioscience, Biotechnology, and Agrochemistry, 23 Dec. 2001, Bioscience, biotechnology, and biochemistry, 65 (12), 2802 - 2805, Russian

  • YAMAUCHI Yasuo, EJIRI Yukinori, SUGIMOTO Toshio, SUEYOSHI Kuni, OJI Yoshikiyo, TANAKA Kiyoshi

    We purified a glutamyl endopeptidase that is a major foliar endopeptidase in cucumber. The endopeptidase had a molecular mass of 400 kDa, consisted of four subunits of 97 kDa, and was inactivated by SH-modifying reagents. Its optimum pH and optimum temperature were 8.0 and 30-37°C, respectively. An internal amino acid sequence of the endopeptidase was highly homologous to a partial sequence of unidentified proteins deduced from genetic information for Arabidopsis thaliana, soybean and rice, but not to the sequences of bacterial glutamyl endopeptidases or animal proteases. Therefore, the unidentified proteins might be glutamyl endopeptidases and be widely distributed only among plant species. The activity of the cucumber glutamyl endopeptidase was inhibited by at least three inhibitors existing in cucumber leaves. One of the inhibitors was a competitive inhibitor of 25 kDa, which did not significantly inhibit commercial endopeptidases derived from animals and microorganisms. This suggests that the cucumber glutamyl endopeptidase might be controlled by endogenous inhibitors in vivo.

    Japanese Biochemical Society, 01 Aug. 2001, Journal of Biochemistry, 130 (2), 257 - 261, English

  • Yamauchi Yasuo, Sugimoto Toshio, Oji Yoshikiyo

    We developed an accurate method to separate and detect proteases using isoelectric focusing electrophoresis. The simultaneous addition of 0.3% octyl-D-glucopylanoside or 8M urea to acrylamide gel containing gelatin and of cytochrome c and pl marker proteins to the sample solution prevented interaction between protease and substrate during electrophoresis. Picogram to nanogram quantities of commercial proteases, papain, chymotrypsin, and proteinase K, were detected at the estimative isoelectric point of these proteases.

    Japan Society for Bioscience, Biotechnology, and Agrochemistry, 23 Apr. 1995, Bioscience, biotechnology, and biochemistry, 59 (4), 767 - 768, English

MISC

  • 植物の香りを農業に活かす : 「植物の言葉」を揮発性バイオスティミュラント

    Corresponding, 2021, 香料, 389, 35 - 40, Japanese

    [Invited]

    Introduction scientific journal

  • 「植物の言葉」を応用したバイオスティミュラント『すずみどり』

    Corresponding, Aug. 2020, 農耕と園藝, 75 (3), 41 - 44, Japanese

    [Invited]

    Introduction commerce magazine

  • 「みどりの香り』が植物を高温から守る〜揮発性バイオスティミュラントを活かした新しい農業〜

    YAMAUCHI YASUO

    2019, Aroma Research, 78 (2), 12 - 17, Japanese

    [Invited]

    Introduction scientific journal

  • Integrated chemical control of abiotic stress tolerance using biostimulants

    YAMAUCHI YASUO

    InTechOpen, Mar. 2018, Plant, Abiotic Stress and Responses to Climate Change, 133 - 144, English

    [Refereed]

    Introduction scientific journal

  • シロイヌナズナの酸化ストレス情報伝達系に関わるシグナル受容体の探索

    東山真理, 青木仁美, 山内靖雄, 中安大, 水谷正治, 杉本幸裕

    2016, 日本農芸化学会関西支部講演会講演要旨集, 497th

  • ついに青葉アルデヒドを作る酵素を捕まえた!〜(Z)-3:(E)-2-ヘキセナールイソメラーゼの同定〜

    YAMAUCHI YASUO

    2016, アロマリサーチ, 17, 46 - 47, Japanese

    [Invited]

    Introduction commerce magazine

  • P079 Identification and characterization of a novel enzyme, hexenal isomerase in response to wounding in plants

    Kunishima Mikiko, Yamauchi Yasuo, Mizutani Masaharu, Sugimoto Yukihiro

    The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2015, 植物化学調節学会研究発表記録集, 50, 97 - 97, Japanese

  • 酸化ストレス応答に関わる新たなプレイヤー 〜活性カルボニル〜

    YAMAUCHI YASUO

    2014, 植物の生長調節, 49, Japanese

    [Invited]

    Introduction scientific journal

  • 59. Gas exchange and stomatal response of root hemi-parasite Striga hermonthica and sorghum under soil water stress

    Inoue Tomoe, Yamauchi Yasuo, Eltayeb Amani Hamad, Samejima Hiroaki, Ueno Kotomi, Babiker Abdel Gabar, Sugimoto Yukihiro

    A pot experiment was conducted to investigate the effects of soil water stress on gas exchange and stomatal response of the upper fully expanded leaf of the root parasitic weed Striga hermonthica and that of its sorghum host. Photosynthetic rate of S. hermonthica was less affected by water stress than sorghum, but the ratio of respiration rate to photosynthetic rate increased considerably under water stress. Thus, under water stress, growth of S. hermonthica may rely more on heterotrophic carbon from host to compensate the low autotrophic carbon gain. Water stress-induced reductions in transpiration rate and stomatal conductance were more severe in infected sorghum than in S. hermonthica. Water stress-induced reduction in stomatal aperture of the leaf was greater in infected sorghum than in S. hermonthica. These results indicated that higher transpiration rate of S. hermonthica under water stress, achieved through greater stomatal aperture, may be related to maintenance of water and solute movement from the host to the parasite.

    The Japanese Society for Chemical Regulation of Plants, 03 Oct. 2011, 植物化学調節学会研究発表記録集, (46), 75 - 75, Japanese

  • Stomatal response and photosynthetic capacity of root parasitic weed Striga and sorghum under different water regimes

    INOUE Tomoe, YAMAUCHI Yasuo, BABIKER Abdel Gabar, ELTAYEB Amani Hamad, SAMEJIMA Hiroaki, SUGIMOTO Yukihiro

    30 Mar. 2011, 日本作物學會紀事, 80, 380 - 381, Japanese

  • 80. Purification and molecular analysis of α,β-unsaturated carbonyl-reducing enzymes from cucumber leaves

    Hasegawa Ayaka, Yamauchi Yasuo, Mizutani Masaharu, Sugimoto Yukihiro

    Polyunsaturated fatty acids in biomembranes are oxidized easily, and result in various low-molecular weight compounds. These compounds include α,β-unsaturated carbonyls like acrolein and methylvinylketone, which are highly reactive. The toxicity of these reactive compounds are based on their ability to form the Michael adducts with thiols and amino groups in proteins and nucleic acids. Therefore, it is important to scavenge α,β-unsaturated carbonyls for maintaining homeostasis in plants. In this study, we purifed the acrolein-reducing enzyme from cucumber leaves and determined its partial amino acid sequences. Based on RT-PCR with degenerate primers and RACE strategies, two cDNA sequences contained fully functional region of the enzyme were obtained. One has chloroplast-targeted transit sequence at N-terminal region, and the other has no. These results indicate that cucumber leaves have α,β-unsaturated carbonyl-detoxifying enzymes localizing in chloroplast and cytosol.

    The Japanese Society for Chemical Regulation of Plants, 01 Oct. 2010, 植物化学調節学会研究発表記録集, (45), 97 - 97, Japanese

  • 11. Involvement of ethylene in Striga hermonthica and Orobanche minor seed germination

    Sawada Ryotaro, Yamauchi Yasuo, Sugimoto Yukihiro

    Seeds of root parasitic plants stay dormant until exposed to warm moist environment (conditioning). The conditioned seeds germinate in response to a germination stimulant. Striga seed germination is also induced by ethylene. The expression of ACC synthase and ACC oxidase genes in S. hermonthica was found to be responsive to the stimulant and to conditioning, respectively. We found that pre-treatment with GR24, a synthetic germination stimulant, during conditioning period decreases S. hermonthica seed germination induced by post-treatment with GR24. The present study was undertaken to investigate the mechanisms involved in the decrease of seed responsiveness to the post-treatment. ACC induced S. hermonthica seed germination in a concentration dependent manner, irrespective of GR24 concentrations in the pre-treatment, suggesting that higher concentrations of GR24 induce ACC biosynthesis. This was supported by the facts that higher concentrations of GR24 induced the expression of the ACC synthase gene and ethylene production. The involvement of ethylene in O. minor seed germination was also studied. Neither ACC nor ethephon induced O. minor seed germination. Germination induced by GR24 was not inhibited by 1-methylcyclopropene. Ethylene release from germinating O. minor seeds was not detected. These facts suggest that ethylene is not involved in O. minor seed germination.

    The Japanese Society for Chemical Regulation of Plants, 06 Oct. 2008, 植物化学調節学会研究発表記録集, (43), 25 - 25, Japanese

  • Physiological and molecular analyses of effects of malondialdehyde during heat stress in Arabidopsis

    NAKAMOTO Chihiro, HIRAOKA Yukihiro, YAMAUCHI Yasuo, SUGIMOTO Yukihiro

    The Japanese Society for Chemical Regulation of Plants, 04 Oct. 2006, 植物の生長調節 = Regulation of plant growth & development, 41, 59 - 59, Japanese

  • Biochemical and molecular biological effects of malondialdehyde formed in heat stressed plants

    Y Yamauchi, K Tanaka, N Wakiuchi, Y Sugimoto

    OXFORD UNIV PRESS, 2006, PLANT AND CELL PHYSIOLOGY, 47, S44 - S44, English

    Summary international conference

  • 47. Interactions between the parasitic weed Striga hermonthica and its host Sorghum bicolor at a molecular level

    Hiraoka Yukihiro, Yamauchi Yasuo, Sugimoto Yukihiro

    Striga spp., root parasites of poaceous plants, devastate cereal production in the semiarid Sub Saharan Africa. The life cycle of the parasite is strongly cued to that of its host and is modulated by host-derived chemical signals. To germinate Striga seeds require conditioning in a warm moist environment and exposure to host derived stimulants, collectively named strigolactones. In this study, host-parasite interactions were analyzed at the molecular level. DNA sequences of clones, isolated by a suppression subtractive hybridization strategy from Striga-parasitized sorghum, were homologous to genes for fatty acid desaturase of maize, heat shock protein 82 of rice, calcium dependent protein kinase of maize and sorghum ESTs induced by biotic and abiotic stresses, including wound, pathogen-infection and oxidative stress.

    The Japanese Society for Chemical Regulation of Plants, 13 Oct. 2005, 植物化学調節学会研究発表記録集, (40), 66 - 66, Japanese

  • Peroxidation of linolenic acid triggered by heat and reactive oxygen species causes protein modification in chloroplasts

    K Seki, Y Yamauchi, K Tanaka, N Wakiuchi, Y Sugimoto

    OXFORD UNIV PRESS, 2005, PLANT AND CELL PHYSIOLOGY, 46, S78 - S78, English

    Summary international conference

  • GLYCATION OF RUBISCO AND ITS INCREASED SUSCEPTIBILITY TO PROTEASES :

    EJIRI Yukinori, YAMAUCHI Yasuo, TANAKA Kiyoshi

    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s75, English

  • Evaluation of sulfite tolerance of active oxygen scavenging enzymes- and sulfur metabolyzing enzymes-related transgenic plants :

    Sasaki Ryozo, Ashiguchi Atsuhiro, Ishida Yoshinori, Oshima Kenta, Yamauchi Yasuo, Tanaka Kiyoshi

    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s124, English

  • Detection of submergence stress-induced genes in submergence tolerant rice cultivar by differential display method and expression mechanim of their genes :

    Osako Masaharu, Kawano Naoyoshi, Ito Osamu, Yamauchi Yasuo, Tanaka Kiyoshi

    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s114, English

  • Detection of high-salt stress-induced genes in high-salt tolerant rice cultivars by differential display method and expression mechanism of their genes :

    Miyata Natsuko, Ito Osamu, Yamauchi Yasuo, Tanaka Kiyoshi

    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s115, English

  • CLONING OF A GENE ENCODING A PROTEIN HOMOLOGOUS TO CUCUMBER GLUTAMYL ENDOPEPTIDASE FROM ARABIDOPSIS THALIANA :

    YAMAUCHI Yasuo, ITAGAKI Tomoko, TANAKA Kiyoshi

    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s204, English

  • Regulation of Endopeptidase in Cucumber Leaves-Regulation by endogenous specific inhibitors :

    YAMAUCHI Yasuo, EJIRI Yukinori, TANAKA Kiyoshi

    Japanese Society of Plant Physiologists, 2000, Plant and cell physiology, 41, s166, English

  • Comparison of phosphoenolpyruvate carboxylases in seeds of maize (Zea mays) and rice (Oryza sativa)

    Sasai Michitaka, Kamata Toru, Yamauchi Yasuo, Sueyoshi Kuni, Sugimoto Toshio, Oji Yoshikiyo

    Kobe University, Mar. 1999, Memoirs of the Graduate School of Science and Technology, Kobe University. A, 17, 11 - 18, English

  • Purification of Two Major Proteases in Cucumber Leaves

    YAMAUCHI Yasuo, ONISHI Michihito, SUGIMOTO Toshio, SUEYOSHI Kuni, OJI Yoshikiyo

    Mar. 1996, Plant and cell physiology, 37, 12 - 12, English

  • Emergence of Proteases in Germinating Cucumber Cotyledons and Their Roles in the Two-Step Degradation of Storage Protein :

    Yamauchi Yasuo, Sugimoto Toshio, Sueyoshi Kuni, Oji Yoshikiyo

    The degradation of storage protein in germinating cu-cumber seeds was shown to proceed via two distinct steps. First, several proteases with acidic isoelectric points (pls) were involved in solubilization and partial degradation of 11S globulin. Treatment of seedlings with cycloheximide inhibited this step and the expression of these proteases. Thus, the first step appeared to be governed by these pro-teases, which were synthesized de novo after imbibition. The first step was observed in dark-grown cotyledons, but the complete degradation of 11S globulin did not occur in the absence of illumination. An additional protease, with a pI of 4.5, was induced by illumination, and it was involved in the further cleavage of the partially degraded products of 11S globulin. Thus, the complete degradation of the storage protein proceeded via a two-step process in illuminated germinating seedlings. Light is needed to induce the second step in the degradation of 11S globulin that supplies the nitrogen required for development of the photosynthetic ap-paratus in the greening cotyledon.

    Japanese Society of Plant Physiologists, 1996, Plant and cell physiology, 37 (3), 279 - 284, English

Books etc

  • 高温環境下で光化学系活性が低下する2つの異なるメカニズムー障害と応答ー

    Apr. 2022, ISBN: 9784860437695

  • 作物の高温耐性を高める揮発性バイオスティミュラント「すずみどり」

    Contributor, Apr. 2022, ISBN: 9784860437695

  • バイオスティミュラントハンドブック

    Supervisor, Apr. 2022, ISBN: 9784860430795

  • 葉の香りによる作物の高温耐性の向上

    Contributor, 農文協, Jan. 2021, ISBN: 9784540200601

  • Function of Extrinsic Proteins in Stabilization of the Photosynthetic Oxygen-Evolving Complex

    Yong Li, Kimura Yukihiro, Ohno Takashi, Yamauchi Yasuo

    Joint work, InTech, 2012, English

    Scholarly book

  • Photosystem at high temperature -mechanisms of adaptation and damage

    Yamauchi Yasuo, Kimura Yukihiro

    Joint work, Nova Science Publishers, 2011, English

    Scholarly book

Presentations

  • 植物由来揮発性化合物を用いた収穫後ベビーリーフの鮮度保持効果の検証

    KOBAYASHI Fumi, KUROKI Shinichiro, YAMAUCHI Yasuo, ITOH Hiromichi

    農業環境工学関連5学会2018年合同大会, Sep. 2018, Japanese, 農業環境工学関連5学会, 松山市、愛媛大学, Domestic conference

    Oral presentation

  • イネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害する

    水本晃那, 小林亜希子, MATSUOKA DAISUKE, YAMAUCHI YASUO, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

    日本光合成学会講演会, 2018, Japanese, Domestic conference

    Poster presentation

  • シロイヌナズナの7TMおよびカルシウムチャネル欠損株を用いた2-hexenal情報伝達系の解析

    AOKI HITOMI, SHINDE HIKARU, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本農芸化学会 関西・中四国・西日本支部2017年度合同大阪大会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

    Oral presentation

  • Keto-enol tautomerism determining plant leaf fragrance

    YAMAUCHI YASUO

    生物物理学会第55回年会, Sep. 2017, English, Domestic conference

    [Invited]

    Invited oral presentation

  • Identification of (Z)-3:(E)-2-hexenal isomerase involved in the production of the leaf aldehyde

    YAMAUCHI YASUO

    第14回日本ナス科コンソーシアム, Sep. 2017, English, 日本大学藤沢キャンパス, International conference

    [Invited]

    Invited oral presentation

  • 酸化ストレス応答性シロイヌナズナ GST の解析

    MATSUURA NAGISA, SAKAI HIROKI, YAMAUCHI YASUO, MANO JUN'ICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    農芸化学会関西支部第499回例会, Jun. 2017, Japanese, 京都府立大学, Domestic conference

    Oral presentation

  • シロイヌナズナの酸化ストレス情報伝達系に関わるシグナル受容体の探索

    HIGASHIYAMA MARI, AOKI HITOMI, YAMAUCHI YASUO, NAKAYASU MASARU, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本農芸化学会関西支部第497回講演会, Dec. 2016, Japanese, Domestic conference

    Oral presentation

  • みどりの香りはストレスシグナルとして機能するか?

    YAMAUCHI YASUO

    植物二次代謝フロンティア研究会, Nov. 2016, Japanese, Domestic conference

    [Invited]

    Invited oral presentation

  • 新規高温耐性誘導剤ヘキセナルを活用したベビーリーフの生産性及び品質向上技術の開発〜ひょうごブランドのベビーリーフ誕生を目指して〜

    YAMAUCHI YASUO

    平成28年度「農」イノベーションひょうご全体交流会, Jun. 2016, Japanese, Domestic conference

    [Invited]

    Nominated symposium

  • 青葉アルデヒドの生成に重要な役割を果たすヘキセナールイソメラーゼの同定

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本植物生理学会第57回年会, Mar. 2016, Japanese, Domestic conference

    Oral presentation

  • 青葉アルデヒドの生成に関わる酵素遺伝子の同定とトマト香気成分の改変

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本農芸化学会2016年度年会, Mar. 2016, Japanese, Domestic conference

    Oral presentation

  • Manipulation of (2E)-hexenal production in plants to elucidate its roles under biotic and abiotic stress condition

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    Pacifichem 2015, Dec. 2015, English, International conference

    Oral presentation

  • Manipulation of (2E)-hexenal production in plants to elucidate its roles under biotic and abiotic stress condition

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    JSBBA KANSAI 2nd Student Forum, Dec. 2015, English, Domestic conference

    Oral presentation

  • 明所高温条件で誘導されるステート2によるPSII障害回避機構の解析

    丸谷 曜子, YAMAUCHI YASUO, 東山 真理, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本植物生理学会, Mar. 2015, Japanese, 東京農業大学, Domestic conference

    Oral presentation

  • 傷害応答に関わる(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本植物生理学会第55回年会, Mar. 2015, Japanese, Domestic conference

    Oral presentation

  • 傷害によって誘導される(3Z):(2E)-ヘキセナールイソメラーゼの同定と機能解析

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本農芸化学会2015年度年会, Mar. 2015, Japanese, Domestic conference

    Oral presentation

  • 酸化ストレスシグナル分子として機能する反応性短鎖緑葉揮発性化合物

    YAMAUCHI YASUO, KUNISHIMA MIKIKO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本植物生理学会第56回年会, Mar. 2015, Japanese, Domestic conference

    Oral presentation

  • 高温ストレスを受けた植物が明条件でPSII障害を回避するメカニズムの解析

    MARUTANI YOKO, YAMAUCHI YASUO, HIGASHIYAMA MARI, AKIMOTO SEIJI, INOUE KANAKO, IKEDA KEN'ICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本光合成学会若手の会, Dec. 2014, Japanese, Domestic conference

    Oral presentation

  • 傷害によって誘導される(3Z):(2E)-ヘキセナールイソメラーゼの同定と酵素学的性質の解明

    KUNISHIMA MIKIKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    第27回植物脂質シンポジウム, Nov. 2014, Japanese, Domestic conference

    Poster presentation

  • 光・温度条件の変化により形成されるPSI-LHCII超複合体の解析

    丸谷曜子, YAMAUCHI YASUO, 井上加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本農芸化学会関西支部会, Sep. 2014, Japanese, 奈良先端科学技術大学, Domestic conference

    Oral presentation

  • コムギの光化学系における高温耐性機構の解析:ステート遷移の誘導と超高速励起型PSI-LHCII複合体の形成

    丸谷 曜子, YAMAUCHI YASUO, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    第5回日本光合成学会年会, May 2014, Japanese, 近畿大学, Domestic conference

    Poster presentation

  • 高温ストレスを受けたコムギにおけるチラコイド膜の構造変化を伴う光化学系の再編成:熱放散とステート遷移の誘導

    丸谷 曜子, YAMAUCHI YASUO, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学, Domestic conference

    Poster presentation

  • アグロインフィルトレーション法による植物発現系を用いた植物代謝酵素の機能解析

    KAWAGUCHI DAICHI, YAMAUCHI YASUO, SUGIMOTO YUKIHIRO, MIZUTANI MASAHARU

    第31回植物脂質シンポジウム, Sep. 2013, Japanese, Domestic conference

    Poster presentation

  • 高温ストレスを受けた植物において再編成される光化学系分子メカニズムの解析

    MARUTANI YOKO, YAMAUCHI YASUO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本植物生理学会第54回年会, Mar. 2013, Japanese, Domestic conference

    Poster presentation

  • Remodeling of photosystems to induce thermal dissipation and state transition accompanied by structural changes of thylakoid membranes in heat-stressed wheat

    Yoko Marutani, Yasuo Yamauchi, Akimoto S, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto

    The 6th Asia and Oceania Conference on Photobiology, 2013, English, International conference

    Poster presentation

  • D1 protein is degraded in heat-stressed plants in the dark, presumably due to electron flow of the stromal-reducing power into the plastoquinone

    MARUTANI YOKO, YAMAUCHI YASUO, KIMURA YUKIHIRO, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    10th International Congress on Plant Molecular Biology, Oct. 2012, English, International conference

    Poster presentation

  • Isolation of ACC synthase gene from Striga gesnerioides

    RNA Babiker, YAMAUCHI YASUO, SUGIMOTO YUKIHIRO

    International Seminar on SATREPS Project for Striga Management, Sep. 2012, English, International conference

    Oral presentation

  • コウモリカズラにおける含塩素イソキノリンアルカノイド生合成中間体の同定

    TAKUMA YUKI, MINAMI MASAKI, NAKASHIMA HITOMI, YAMAUCHI YASUO, MIZUTANI MASAHARU, TAKIKAWA HIROSATO, SUGIMOTO YUKIHIRO

    平成25年度日本植物細胞分子学会, Aug. 2012, Japanese, Domestic conference

    Poster presentation

  • Gas exchange and stomatal response of root parasitic weed Striga hermonthica and sorghum under water stress

    INOUE TOMOE, YAMAUCHI YASUO, AMANI HAMAD ELTAYEB, SAMEJIMA HIROAKI, ABDEL GABAR BABIKER, SUGIMOTO YUKIHIRO

    International Society of Root Research, Jun. 2012, English, International conference

    Oral presentation

  • 高温ストレスを受けた植物がステート遷移によりPSIIを保護するメカニズムの解析

    MARUTANI YOKO, YAMAUCHI YASUO, AKIMOTO SEIJI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO

    日本植物生理学会第53回年会, Mar. 2012, Japanese, Domestic conference

    Poster presentation

  • 高温ストレスを受けた植物がステート遷移によりPSIIを保護するメカニズムの解析

    Marutani Yoko, Yamauchi Yasuo, Akimoto Seiji, Kimura Yukihiro, Mizutani Masaharu, Sugimoto Yukihiro

    第53回日本植物生理学会年会, 2012, Japanese, Domestic conference

    Oral presentation

  • Translocation of host materials to parasite: Stomatal response and photosynthetic capacity of Striga hermonthica and sorghum under water stress

    T. Inoue, Y. Yamauchi, H. E. Amani, H. Samejima, K. Ueno, A. G. T. Babiker, Y. Sugimoto

    International Seminar on SATREPS Project for Striga Management, 2012, English, Khartoum, Sudan, International conference

    Oral presentation

  • 植物が光化学系の高温障害を回避するメカニズムの解析

    Marutani Yoko, Yamauchi Yasuo, Akimoto Seiji, Kimura Yukihiro, Mizutani Masaharu, Sugimoto Yukihiro

    日本農芸化学会関西・中部支部合同大会, 2011, Japanese, Domestic conference

    Oral presentation

  • 根寄生植物-宿主植物間相互作用の分子解析(Ⅰ)ストライガの寄生に対するソルガムの応答

    平岡 幸浩, 山内 靖雄, 杉本 幸裕

    植物化学調節学会, 2005, Japanese, 植物化学調節学会, 東京大学, Domestic conference

    Oral presentation

  • 高温と活性酸素が引き起こすリノレン酸の過酸化が葉緑体タンパク質の化学修飾をもたらす

    関 久美子, 山内 靖雄, 田中 浄, 脇内 成昭, 杉本 幸裕

    日本植物生理学会年会, 2005, Japanese, 日本植物生理学会, 新潟, Domestic conference

    Oral presentation

  • 冠水耐性イネからの新規冠水誘導性遺伝子の単離と発現

    斉 艶華, 山内 靖雄, 凌 建群, 河野 尚由, 李 徳葆, 田中 浄

    日本植物生理学会年会, 2005, Japanese, 日本植物生理学会, 新潟, Domestic conference

    Oral presentation

  • Molecular interactions between the parasitic weed Striga hermonthica and its host Sorghum bicolor

    HIRAOKA Yukihiro, MIYAMOTO Sumiyo, YAMAUCHI Yasuo, SUGIMOTO Yukihiro

    Pacifichem2005, 2005, English, 未記入, 未記入, International conference

    Oral presentation

  • 植物生体膜構成多価不飽和脂肪酸の過酸化によるタンパク質の化学修飾

    山内 靖雄, 豊田 泰之, 田中 浄

    日本植物生理学会, 2004, Japanese, 日本植物生理学会, 東京都立大学, Domestic conference

    Oral presentation

  • ミヤコグサ培養根が生産する根寄生植物種子発芽刺激物質の精製

    上山 知樹, 伊東 ちひろ, 山内 靖雄, 脇内 成昭, 杉本 幸裕

    日本農芸化学会関西支部例会第437回講演会, 2004, Japanese, 日本農芸化学会関西支部, 神戸大学, Domestic conference

    Oral presentation

  • コウモリカズラ培養根からのdechlorodauricumineの単離・構造決定

    松井 美春, 滝川 浩郷, 佐々木 満, 山内 靖雄, 脇内 成昭, 杉本 幸裕

    日本農芸化学会関西支部第437回講演会, 2004, Japanese, 日本農芸化学会関西支部, 神戸大学, Domestic conference

    Oral presentation

Research Projects

  • Sensing of volatile organic compounds by plants: Molecular mechanisms and ecological functions

    高林 純示, 塩尻 かおり, 米谷 衣代, 松井 健二, 山内 靖雄, 村田 純

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kyoto University, 01 Apr. 2022 - 31 Mar. 2027

  • Biochemical mechanism of reactive carbonyl signaling in plants

    真野 純一, 杉本 貢一, 村田 芳行, 深城 英弘, 武宮 淳史, 山内 靖雄

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Yamaguchi University, 01 Apr. 2020 - 31 Mar. 2023

    1) 青色光による気孔開口をRCSが阻害する現象を発見し,青色光シグナル伝達経路におけるRCSの作用点はH+ATPaseの活性化メカニズムの近傍であることを明らかにした。(論文投稿中) 2) ホウレンソウ葉緑体チラコイド膜,包膜に存在するカルボニル種を解析し,包膜には主としてリポキシゲナーゼ反応に由来するC6アルデヒドが存在するのに対し,チラコイド膜にはC3-C11の多様なカルボニル種が含まれており,それらのチラコイド膜内の濃度はmMレベルであることを明らかにした。一方,ストロマに存在するカルボニル種の濃度は極めて低く,ストロマでは可溶性のカルボニル消去物質及び消去酵素によってRCSレベルが極めて低く抑えられていることを明らかにした。(論文投稿中) 3) 植物がもつRCS消去物質として,新たにニンニクからアリインを同定した。アリインはアミノ基にRCSを2分子付加させて消去すること,またそのRCS消去能は,動物由来のRCS消去ペプチドであるカルノシンより高いことを明らかにした。(論文執筆中) 4) RCS消去酵素2-アルケナールレダクターゼ(AER)を葉緑体に局在させる組換えシロイヌナズナを作成した。また,アクロレインを特異的に消去するグルタチオントランスフェラーゼ(GST)アイソザイムTau19(GSTU19)を葉緑体及び細胞質に局在させる組換えシロイヌナズナを作成した。さらにアクロレインと4-ヒドロキシノネナール(HNE)に特異的なGSTU17を葉緑体,細胞質,アポプラストに局在させる組換えシロイヌナズナを作成した。 5) 高塩分が引き起こす酸化ストレスにおいて,アクロレイン,HNE, (E)-2-ペンテナール植物体内で増大し発芽や生長を抑制することを明らかにした。(論文投稿中) 6) 植物の小胞体ストレスに伴いRCSが増大することを明らかにした。(論文投稿中)

  • Plant-human communication by plant volatile compounds and its application for prolonging the shelf life of leafy vegetables

    KUROKI Shinichiro

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 28 Jun. 2019 - 31 Mar. 2022

    The effects of artificial exposure to green leaf volatiles (GLVs) on yellowing, quality deterioration, and pathogen resistance of green leaves were investigated. Results showed that trans-2-hexenal has a harmful effect, whereas cis-3-hexenyl acetate has a favorable influence in reducing yellowing and weight loss, and improving resistance against Colletotrichum higginsianum.

  • How do plants utilize reactive carbonyl species as specific redox signals?

    MANO Jun'ichi

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Yamaguchi University, 01 Apr. 2017 - 31 Mar. 2020

    Reactive carbonyl species (RCS), decomposition products of lipid peroxides, mediate oxidative damage and oxidative signal in plants as downstream products of reactive oxygen species (ROS). To elucidate the regulation and function of RCS signal, we examined their metabolism and actions. Following results were obtained. (1) The enzymatic activity of 20 tau isozymes of glutathione transferase (GST) for RCS were examined. A half the tested isozymes scavenged RCS, suggesting that RCS are major endogenous substrates of GST. (2) Addition of ROS or RCS to Arabidopsis thaliana roots induced auxin-responsive genes and induced lateral root (LR). Thus ROS and its downstream products RCS constitute a feed-forward loop of the auxin signal for LR formation. (3) In ROS-triggered programmed cell death (PCD) of tobacco cells, an RCS reductase was found inactivated by ROS at a very early stage. This suggests that the reductase is a ROS sensor to facilitate RCS signal for PCD.

  • 杉本 幸裕

    科学研究費補助金/基盤研究(B), Apr. 2015 - Mar. 2019

    Competitive research funding

  • 山内 靖雄

    学術研究助成基金助成金/基盤研究(C), Apr. 2015 - Mar. 2018, Principal investigator

    Competitive research funding

  • 植物の高温耐性誘導剤の実用化に向けた実証試験

    山内 靖雄

    研究成果展開事業(マッチングプランナー プログラム), 2016, Principal investigator

    Competitive research funding

  • 新規高温耐性誘導剤ヘキセナルを活用したベビーリーフの生産性及び品質向上技術の開発

    山内 靖雄

    兵庫県, 「農」イノベーションひょうご研究開発プロジェクト支援事業, 2015, Principal investigator

    Competitive research funding

  • マッチングプランナー「植物の高温耐性誘導剤の実用化に向けた実証試験」

    山内 靖雄

    科学技術振興機構, 研究成果展開事業 マッチングプランナープログラム 探索試験, 2015, Principal investigator

    Competitive research funding

  • 山内 靖雄

    学術研究助成基金助成金/基盤研究(C), 2011, Principal investigator

    Competitive research funding

  • 山内 靖雄

    科学研究費補助金/挑戦的萌芽研究, 2009, Principal investigator

    Competitive research funding

  • 杉本 幸裕

    科学研究費補助金/基盤研究(B), 2008

    Competitive research funding

  • Metabolism of lipid peroxide-derived aldehydes in chloroplasts and their physiological roles in environmental stress

    MANO Jun'ichi, YAMAUCHI Yasuo

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Yamaguchi University, 2006 - 2007

    Environmental stresses such as heat, drought and UV-B commonly lead to oxidative damage in leaf cells due to enhanced production of reactive oxygen species in chloroplasts. Recently, lipid peroxide-derived aldehydes have been recognized to mediate cell injury, but their metabolism and toxicology in plants are poorly investigated. In this study we investigated (i) the metabolism of lipid peroxide-derived aldehydes in the chloroplast and (ii) the physiological roles of those aldehydes in the plant responses to environmental stresses. We have obtained the following results : (1) Various species of aldehydes inactivated the CO2-fixation in isolated chloroplasts in vitro. The toxicity was higher for 2-alkenals than n-alkanals. Acrolein and 4-hydroxynonenal showed the greatest toxicity. Several enzymes in the Calvin cycle by 2-alkenals, but the thylakoid electron transport chain was much less affected. (2) Glutathione supplemented to chloroplasts retarded the aldehyde-induced loss of CO_2-fixation, but ascorbate did not, showing the specific role of glutathione to scavenge aldehydes. Aldehydes were extracted from distinct compartments in the chloroplast, derivatized with 2,4-dinitrophenylhydrazine and determined with HPLC. Lipoxygenase pathway-dependent C6 aldehydes were major components found in envelopes and stroma. Thylakoids contained greater variety of aldehydes, including highly toxic 2-alkenals and 4-hydroxy-2-alkenals, suggesting non-enzymatic(reactive oxygen species-mediated)production of aldehydes in thylakoids. (3) Aldehyde composition in the linolenic acid-deficient Arabidopsis mutant was compared with that in wild type. Acrolein, propionaldehyde malondialdehyde and 2E-pentenal were derived from linolenic acid. (4) When Arabidopsis plants were heat stressed or tobacco leaves were strongly illuminated, aldehyde-modification of several chloroplast enzymes in them were increased. One of the malondialdehyde-modified proteins was OEC33 protein, suggesting the production of malondialdehyde in the lumen. Thus heat- or light-stress induces the production highly toxic aldehydes from lipid peroxides in chloroplasts, leading to inactivation of the Calvin cycle. Glutathione is a critically important detoxicant against aldehydes.

  • 杉本 幸裕

    科学研究費補助金/基盤研究(B), 2005

    Competitive research funding

  • 杉本 幸裕

    科学研究費補助金/基盤研究(C), 2005

    Competitive research funding

  • アクチベーションタギング法による環境ストレス耐性植物の作製と原因遺伝子の単離

    山内 靖雄, 田中 浄

    日本学術振興会, 科学研究費助成事業, 萌芽的研究, 鳥取大学, 1998 - 1999

    本研究では、強力な遺伝子転写プロモーターを植物に人為的に導入し、無作為に種々の植物ゲノム遺伝子の転写を活性化させることにより、さまざまな転写活性型突然変異体を作製し、原因となっている転写活性化された遺伝子のクローニングをするアクチベーションタギング法を用いて、環境ストレス耐性に関与する遺伝子をクローニングすることを最終目的として実験を行った。 実験材料は当初はタバコとシロイヌナズナを用いて行ったが、シロイヌナズナを用いる形質転換係が芳しくなく、効率的に変異体を作製することができなかったので、当研究室で確立しているタバコを用いた形質転換系を用いて変異体植物作製を集中的に行った。 本実験ではアグロバクテリウム(GV3101)とアクチベーションタギング用プラスミド(pPCVICEn4HPT)を組み合わせ、タバコ葉片に感染法によりプロモーターを導入した。プロモーター導入後のタバコカルスを抗生物質(ハイグロマイシン)を含んだ培地で選抜し、変異体植物タバコを完全体に再生した。第一世代の形質転換体タバコは種子を得るため、約6ヶ月間種子が完熟するまで栽培した。その結果、計191系統の形質転換体タバコを得ることができた。現在、得られた突然変異体タバコを播種し、塩ストレス、乾燥ストレスを負荷し、ストレスに耐性を持った個体を選抜中である。また、これらの形質転換体タバコのうちいくつかは、形態的に野生株と異なっているものがあり、今後はこのようなタバコ個体の遺伝子解析も行う予定である。

  • CLARIFICATION OF MECHANISM OF SUBMERGENCE-TOLERANCE USING A SUBMERGENCE-TOLERANT RICE CULTIVAR

    TANAKA Kiyoshi, YAMAUCHI Yasuo

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), TOTTORI UNIVERSITY, 1998 - 1999

    The visual damage of submergence usually develops after desubmergence. A part of the damage may be caused by active oxygen produced in transition of oxygen environment. The submergence-intolerant rice cultivar, IR42 suffered from more lipid peroxidation with the production of HィイD22ィエD2OィイD22ィエD2 than submergence-tolerant cultivar, FR13A, after desubmergence. The activities of active oxygen scavenging enzymes in both cultivars were about the same or higher relative to the nonsubmerged control. After 6d of submergence, the total ascorbate levels in both cultivars decreased by 81% and 55% for IR42 and FR13A, respectively. During 7d of recovery after desubmergence, however, FR13A increased level of total ascorbate up to 150% while IR42 did it up to only 78%. Moreover, the proportion of ascorbic acid to total ascorbate I FR13A increased significantly after 3d of recovery and reached the level of that in nonsubmerged control, but the proportion in IR42 was still low during the recovery phase. It means that FR13A could operate the AOS scavenging system better than IR42 after desubmergence. It is suggested that the AOS may be one of the causes of the damage after desubmergence and ascorbate may take ana active role in alleviating the damage. Three cDNA fragments were isolated by differential screening between FR13A desubmerged for 2days after seven days' submergence was cancelled, and non-submerged control FR13A. The one was a giberellin-synthesis-related gene, the second was a proteasome-related gene, and the third was a tolerant gene to pathogenic microorganisms.