Directory of Researchers

SASAZAKI Shinji
Graduate School of Agricultural Science / Department of Bioresource Science
Associate Professor
Agriculture
Last Updated :2024/02/14

Researcher Profile and Settings

Affiliation

  • <Faculty / Graduate School / Others>

    Graduate School of Agricultural Science / Department of Bioresource Science
  • <Related Faculty / Graduate School / Others>

    Faculty of Agriculture / Department of Bioresource Science

Teaching

Research Activities

Research Areas

  • Life sciences / Animal production science

Awards

  • Sep. 2021 日本畜産学会, 2021年度日本畜産学会賞, 兵庫県黒毛和種集団における肉質関連遺伝子の同定

    笹崎晋史

  • Mar. 2011 日本畜産学会, 日本畜産学会奨励賞, 牛肉の品種及び産地を識別するDNAマーカーの開発

    SASAZAKI SHINJI

    Japan society

Published Papers

  • Shinji Sasazaki, Hina Kondo, Yurika Moriishi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

    Abstract Background Beef marbling is considered a desirable trait in the meat industry. Therefore, understanding the genetic factors that cause marbling is important. Previously, we performed a genome-wide association study to examine genetic factors associated with beef marbling in Japanese Black cattle and identified a candidate region between 10–30 Mbp on chromosome 7. We verified the effect of the SNPs in this region on beef marbling using linkage disequilibrium block analysis. We narrowed down the candidate region to a range of 15.8–16.1 Mbp. In this study, we comprehensively detected all of the SNPs in this region and verified their effects on beef marbling. Results Genome resequencing using four animals exhibiting high beef marbling standard (BMS) and four with low BMS revealed a total of 1,846 polymorphisms within the candidate region. Based on the annotation, we selected 13 SNPs exhibiting a moderate impact, as no high-impact SNPs were detected. All of the SNPs represented missense polymorphisms and were located in the following seven genes: RDH8, ANGPTL6, DNMT1, MRPL4, ICAM1, ICAM3, and ICAM5. Finally, we determined the effects of these SNPs on the BMS of a Japanese Black cattle population (n = 529). Analysis of variance revealed that the five SNPs were located in genes encoding the intercellular adhesion molecules (ICAM1, ICAM3, and ICAM5), and showed a highly significant association compared with the remainder (p < 0.01). The lowest p-value was observed for ICAM3_c.739G > A (p = 1.18E-04). Previous studies have suggested that intercellular adhesion molecules (ICAM) may be an upstream factor that regulates adipocyte differentiation. Therefore, considering the polymorphism and putative gene function, we suggest that ICAM1 is potentially responsible for beef marbling. c.470C > G and/or c.994G > A on ICAM1 may be responsible for this quantitative trait locus. Conclusions Promising SNP candidates responsible for beef marbling were identified using extensive polymorphism verification in a previously reported QTL region. We aim to elucidate the mechanism of beef marbling in future studies by investigating how these polymorphisms alter protein structure and function.

    Springer Science and Business Media LLC, 09 Feb. 2024, BMC Genomic Data, 25 (1), 17

    [Refereed]

    Scientific journal

  • Fuki KAWAGUCHI, Mitsuki NAKAMURA, Keiichi SUZUKI, Eiji KOBAYASHI, Shinji SASAZAKI, Hideyuki MANNEN

    Wiley, Oct. 2022, Animal Science Journal, 93 (1), e13770, English

    [Refereed]

    Scientific journal

  • Shuji Ueda, Yasuharu Takashima, Yunosuke Gotou, Ryo Sasaki, Rio Nakabayashi, Takeshi Suzuki, Shinji Sasazaki, Ituko Fukuda, Biniam Kebede, Yuki Kadowaki, Maiko Tamura, Hiroki Nakanishi, Yasuhito Shirai

    Japanese Black cattle (Japanese Wagyu) beef is attracting attention for its aroma and marbling, and its handling is increasing worldwide. Here, we focused on the origin discrimination of Wagyu beef and analyzed the nutritional components of Japanese Wagyu (produced in multiple prefectures of Japan), Hybrid Wagyu (a cross between Angus and Wagyu cattle born in Australia and transported to Japan), and Australian Wagyu beef using mass spectrometry (MS). Triple-quadrupole liquid chromatography-MS was used to clarify the molecular species of lipids in Wagyu beef. Fourteen classes of lipids were separated, and 128 different triacylglycerides (TGs) were detected. A simple comparative analysis of these TGs using high-performance liquid chromatography revealed significantly higher levels of triolein (C18:1/C18:1/C18:1; abbreviated OOO) and C18:1/C18:1/C16:1 (OOPo) in Japanese Wagyu. Wagyu elements beef were comprehensively analyzed using inductively coupled plasma (ICP)-MS and ICP-optical emission spectrometry. We found significant differences in the rubidium, cesium, and lithium levels of Japanese and Australian Wagyu beef. On comparing metabolites using gas chromatography-MS, we identified significant differences in the levels of amino acids and other components of the Japanese and Australian Wagyu beef. These results suggest the possibility of determining the origin of Wagyu cattle breeds using MS and genetic discrimination.

    Aug. 2022, Metabolites, 12 (9), 777, English, International magazine

    [Refereed]

    Scientific journal

  • Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

    In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.

    MDPI AG, 01 Jul. 2022, Genes, 13 (7), 1190 - 1190

    [Refereed]

    Scientific journal

  • Var Goats Consortium, Isaäc J. Nijman, Benjamin D. Rosen, Philippe Bardou, Thomas Faraut, Tristan Cumer, Kevin G. Daly, Zhuqing Zheng, Yudong Cai, Hojjat Asadollahpour, Bengi Çınar Kul, Wei‐Yi Zhang, E. Guangxin, A. Ayin, Hayley Baird, Meirat Bakhtin, Valentin A. Bâlteanu, Diana Barfield, Beate Berger, Thor Blichfeldt, Geert Boink, Sri R. A. Bugiwati, Zexi Cai, Sean Carolan, Emily Clark, Vlatka Cubric‐Curik, Muhammad I. A. Dagong, Tashi Dorji, Louise Drew, Jiazhong Guo, Jón Hallsson, Simon Horvat, Juha Kantanen, Fuki Kawaguchi, Polat Kazymbet, Negar Khayatzadeh, Namshin Kim, Manoj Kumar Shah, Yuying Liao, Amparo Martínez, Joseph S. Masangkay, Maho Masaoka, Raffaele Mazza, John McEwan, Marco Milanesi, Md. Omar Faruque, Yuto Nomura, Nadjet‐Amina Ouchene‐Khelifi, Filipe Pereira, Goutam Sahana, Mazdak Salavati, Shinji Sasazaki, Anne Da Silva, Mojca Simčič, Johann Sölkner, Alison Sutherland, Johannes Tigchelaar, Hongpin Zhang, Econogene Consortium, Paolo Ajmone‐Marsan, Daniel G. Bradley, Licia Colli, Cord Drögemüller, Yu Jiang, Chuzhao Lei, Hideyuki Mannen, François Pompanon, Gwenola Tosser‐Klopp, Johannes A. Lenstra

    Wiley, 25 Jun. 2022, Molecular Ecology, 31 (16), 4364 - 4380

    Scientific journal

  • Kohei Nishikaku, Takahiro Yonezawa, Masahide Nishibori, Masashi Harada, Fuki Kawaguchi, Shinji Sasazaki, Yasushi Torii, Kazuhiko Imakawa, Kuniko Kawai, Jianquan Liu, Hideyuki Mannen, Tomoko Kobayashi

    Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the Bos genus were domesticated in Asia, where they also diversified. As native cattle (taurine cattle, zebu cattle, yak, and water buffalo) are indigenous and adapted to local environments, we hypothesized that Asian native cattle could have harbored BLV and, therefore, that they were important for virus emergence, maintenance, and spread. In this study, phylogeographic and ancestral trait analyses—including sequences obtained from Asian native cattle—were used to reconstruct the evolutionary history of BLV. It was shown that, since its probable emergence in Asia, the virus spread to South America and Europe via international trade of live cattle. It was inferred that zebu cattle were the hosts for the early origin of BLV, while taurine cattle played the significant role in the transmission worldwide. In addition, the results of positive selection analysis indicate that yak had a substantially minor role in the transmission of this virus. In this study, endogenous deltaretrovirus sequences in bats, collected in Asian countries, were also analyzed on whether these sequences were present in the bat genome. Endogenous deltaretrovirus sequences were detected from bat species endemic to specific regions and geographically isolated for a long time. Endogenous deltaretrovirus sequences from these geographically isolated species represent ancient exogenous deltaretroviruses distributions. The phylogenetic analysis revealed that these newly obtained endogenous deltaretrovirus sequences were closely related to those of BLV from Asian native cattle, indicating that BLV-related ancient deltaretroviruses circulated in Asia long before the emergence of BLV. Together, our analyses provide evidence for origin and spatiotemporal dynamics of BLV.

    Frontiers Media SA, 24 Jun. 2022, Frontiers in Microbiology, 13

    Scientific journal

  • Development of DNA markers for improvement of meat quality in a Japanese Black cattle population in Hyogo Prefecture

    Shinji Sasazaki

    Dec. 2021, Animal Science Journal, 92 (1)

    [Refereed][Invited]

  • Shinji Sasazaki, Koichiro Tomita, Yuto Nomura, Fuki Kawaguchi, Tetsuo Kunieda, Manoj Kumar Shah, Hideyuki Mannen

    Lead, Wiley, 28 Sep. 2021, Animal Science Journal, 92 (1)

    [Refereed]

    Scientific journal

  • Recent achievements of candidate polymorphism detection for fatty acid composition in Japanese Black cattle

    Fuki KAWAGUCHI, Hideyuki MANNEN, Shinji SASAZAKI

    Corresponding, Aug. 2021, The Journal of Animal Genetics, 49, 67 - 75, English

    [Refereed]

  • Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.

    Corresponding, MDPI AG, 22 Jun. 2021, Life, 11 (7), 597 - 597

    [Refereed]

    Scientific journal

  • M. Zahangir Alam, Yun-Mi Lee, Hyo-Jung Son, Lauren H. Hanna, David G. Riley, Hideyuki Mannen, Shinji Sasazaki, Se Pill Park, Jong-Joo Kim

    Objective: Conservation and genetic improvement of cattle breeds require information about genetic diversity and population structure of the cattle. In this study, we investigated the genetic diversity and population structure of the three cattle breeds in the Korean peninsula.Methods: Jeju Black, Hanwoo, Holstein cattle in Korea, together with six foreign breeds were examined. Genetic diversity within the cattle breeds was analyzed with minor allele frequency (MAF), observed and expected heterozygosity (HO and HE), inbreeding coefficient (FIS) and past effective population size. Molecular variance and population structure between the nine breeds were analyzed using a model-based clustering method. Genetic distances between breeds were evaluated with Nei’s genetic distance and Weir and Cockerham’s FST.Results: Our results revealed that Jeju Black cattle had lowest level of heterozygosity (HE = 0.21) among the studied taurine breeds, and an average MAF of 0.16. The level of inbreeding was –0.076 for Jeju Black, while –0.018 to –0.118 for the other breeds. Principle component analysis and neighbor-joining tree showed a clear separation of Jeju Black cattle from other local (Hanwoo and Japanese cattle) and taurine/indicine cattle breeds in evolutionary process, and a distinct pattern of admixture of Jeju Black cattle having no clustering with other studied populations. The FST value between Jeju Black cattle and Hanwoo was 0.106, which was lowest across the pair of breeds ranging from 0.161 to 0.274, indicating some degree of genetic closeness of Jeju Black cattle with Hanwoo. The past effective population size of Jeju Black cattle was very small, i.e. 38 in 13 generation ago, whereas 209 for Hanwoo.Conclusion: This study indicates genetic uniqueness of Jeju Black cattle. However, a small effective population size of Jeju Black cattle indicates the requirement for an implementation of a sustainable breeding policy to increase the population for genetic improvement and future conservation.

    Asian Australasian Association of Animal Production Societies, 01 May 2021, Animal Bioscience, 34 (5), 789 - 800

    [Refereed]

    Scientific journal

  • Indonesian native goats (Capra hircus) reveal highest genetic frequency of mitochondrial DNA haplogroup B in the world

    Hideyuki Mannen, Kenta Iso, Fuki Kawaguchi, Shinji Sasazaki, Takahiro Yonezawa, Muhammad Ihsan Andi Dagong, Sri Rachma Aprilita Bugiwati

    Nov. 2020, Animal Science Journal

    [Refereed]

  • Hideyuki Mannen, Takahiro Yonezawa, Kako Murata, Aoi Noda, Fuki Kawaguchi, Shinji Sasazaki, Anna Olivieri, Alessandro Achilli, Antonio Torroni

    AbstractSurveys of mitochondrial DNA (mtDNA) variation have shown that worldwide domestic cattle are characterized by just a few major haplogroups. Two, T and I, are common and characterize Bos taurus and Bos indicus, respectively, while the other three, P, Q and R, are rare and are found only in taurine breeds. Haplogroup P is typical of extinct European aurochs, while intriguingly modern P mtDNAs have only been found in northeast Asian cattle. These Asian P mtDNAs are extremely rare with the exception of the Japanese Shorthorn breed, where they reach a frequency of 45.9%. To shed light on the origin of this haplogroup in northeast Asian cattle, we completely sequenced 14 Japanese Shorthorn mitogenomes belonging to haplogroup P. Phylogenetic and Bayesian analyses revealed: (1) a post-glacial expansion of aurochs carrying haplogroup P from Europe to Asia; (2) that all Asian P mtDNAs belong to a single sub-haplogroup (P1a), so far never detected in either European or Asian aurochs remains, which was incorporated into domestic cattle of continental northeastern Asia possibly ~ 3700 years ago; and (3) that haplogroup P1a mtDNAs found in the Japanese Shorthorn breed probably reached Japan about 650 years ago from Mongolia/Russia, in agreement with historical evidence.

    Springer Science and Business Media LLC, Nov. 2020, Scientific Reports, 10 (1)

    [Refereed]

    Scientific journal

  • Fuki Kawaguchi, Miyako Tsuchimura, Kenji Oyama, Tamako Matsuhashi, Shin Maruyama, Hideyuki Mannen, Shinji Sasazaki

    Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.

    Corresponding, Copernicus GmbH, 13 Jan. 2020, Archives Animal Breeding, 63 (1), 9 - 17

    [Refereed]

    Scientific journal

  • Shinji Sasazaki, Fuki Kawaguchi, Ayaka Nakajima, Raito Yamamoto, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen

    Wiley, Jan. 2020, Animal Science Journal, 91 (1), e13335

    [Refereed]

    Scientific journal

  • Matsumoto H, Kohara R, Sugi M, Usui A, Oyama K, Mannen H, Sasazaki S

    Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.

    Last, Dec. 2019, Heliyon, 5 (12), e03006, English, International magazine

    [Refereed]

    Scientific journal

  • Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Felix Rakotondraparany, Fanomezana Mihaja Ratsoavina, Takahiro Yonezawa, Hideyuki Mannen

    Zoological Society of Japan, 01 Aug. 2019, Zoological Science, 36 (4), 294 - 294, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Fuki Kawaguchi, Shotaro Itoh, Suzuna Yotsu, Kento Fukuda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Last, Elsevier BV, Jun. 2019, Meta Gene, 20, 100558 - 100558

    [Refereed]

    Scientific journal

  • Fuki Kawaguchi, Hiroto Kigoshi, Moriyuki Fukushima, Eiji Iwamoto, Eiji Kobayashi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Corresponding, Wiley, Apr. 2019, Animal Science Journal, 90 (4), 467 - 472, English

    [Refereed]

    Scientific journal

  • 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

    山本 雷斗, 川口 芙岐, 秋山 敬孝, 吉田 恵実, 小浜 菜美子, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

    (公社)日本畜産学会, Mar. 2019, 日本畜産学会大会講演要旨集, 125回, 177 - 177, Japanese

  • Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Meirat Bakhtin, Polat Kazymbet, Alykhan Meldevekob, Maratbek Z. Suleimenov, Masahide Nishibori, Hideyuki Mannen

    Wiley, Mar. 2019, Animal Science Journal, 90 (3), 317 - 322, English

    [Refereed]

    Scientific journal

  • Hiroto Kigoshi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Corresponding, Japanese Society of Animal Breeding and Genetics, 2019, The Journal of Animal Genetics, 47 (2), 37 - 45

    [Refereed]

    Scientific journal

  • Hayate Yamanaka, Kako Murata, Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Meirat Bakhtin, Polat Kazymbet, Alykhan Meldevekob, Maratbek Z. Suleimenov, Masahide Nishibori, Hideyuki Mannen

    Wiley, Jan. 2019, Animal Science Journal, 90 (1), 29 - 34, English

    [Refereed]

    Scientific journal

  • 日本ホルスタインにおいて観察された稀少なmtDNAハプログループP

    野田葵, 川口 芙岐, 笹崎晋史, 万年英之

    Aug. 2018, 動物遺伝育種研究, 46 (2), 49 - 55, Japanese

    [Refereed]

    Scientific journal

  • Fuki Kawaguchi, Hiroto Kigoshi, Ayaka Nakajima, Yuta Matsumoto, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Corresponding, Wiley, Aug. 2018, Animal Science Journal, 89 (8), 1060 - 1066, English

    [Refereed]

    Scientific journal

  • Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析

    山中 颯, 笹崎晋史, Hla Hla Moe, Moe Lwin, 下桐 猛, 万年英之

    Aug. 2018, 日本畜産学会報, 89 (3), 313 - 321, Japanese

    [Refereed]

    Scientific journal

  • Ayaka Nakajima, Fuki Kawaguchi, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Corresponding, Wiley, May 2018, Animal Science Journal, 89 (5), 743 - 751, English

    [Refereed]

    Scientific journal

  • ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

    川口 芙岐, 木越 洸利, 小浜 菜美子, 秋山 敬孝, 福島 護之, 吉田 恵実, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

    (公社)日本畜産学会, Mar. 2018, 日本畜産学会大会講演要旨集, 124回, 111 - 111, Japanese

  • Aoi NODA, Fuki KAWAGUCHI, Shinji SASAZAKI,, Hideyuki MANNEN

    Japanese Society of Animal Breeding and Genetics, 2018, The Journal of Animal Genetics, 46 (2), 49 - 55

    [Refereed]

    Scientific journal

  • Aoi Noda, Riku Yonesaka, Shinji Sasazaki, Hideyuki Mannen

    Background Aurochs (Bos primigenius) were distributed throughout large parts of Eurasia and Northern Africa during the late Pleistocene and the early Holocene, and all modern cattle are derived from the aurochs. Although the mtDNA haplogroups of most modern cattle belong to haplogroups T and I, several additional haplogroups (P, Q, R, C and E) have been identified in modern cattle and aurochs. Haplogroup P was the most common haplogroup in European aurochs, but so far, it has been identified in only three of > 3,000 submitted haplotypes of modern Asian cattle. Methodology We sequenced the complete mtDNA D-loop region of 181 Japanese Shorthorn cattle and analyzed these together with representative bovine mtDNA sequences. The haplotype P of Japanese Shorthorn cattle was analyzed along with that of 36 previously published European aurochs and three modern Asian cattle sequences using the hypervariable 410 bp of the D-loop region. Conclusions We detected the mtDNA haplogroup P in Japanese Shorthorn cattle with an extremely high frequency (83/181). Phylogenetic networks revealed two main clusters, designated as Pa for haplogroup P in European aurochs and Pc in modern Asian cattle. We also report the genetic diversity of haplogroup P compared with the sequences of extinct aurochs. No shared haplotypes are observed between the European aurochs and the modern Asian cattle. This finding suggests the possibility of local and secondary introgression events of haplogroup P in northeast Asian cattle, and will contribute to a better understanding of its origin and genetic diversity.

    Public Library of Science, 01 Jan. 2018, PLoS ONE, 13 (1), e0190937, English

    [Refereed]

    Scientific journal

  • Fuki Kawaguchi, Yuto Kitamura, Ryuji Nakajima, Masayoshi Takahashi, Hiroaki Goto, Yuichi Washida, Yoshikazu Yamamoto, Shinji Sasazaki, Hideyuki Mannen

    Corresponding, Wiley, Jan. 2018, Animal Science Journal, 89 (1), 257 - 258, English

    [Refereed]

    Scientific journal

  • H. Kigoshi, F. Kawaguchi, R. Yasuzumi, K. Oyama, H. Mannen, S. Sasazaki

    Corresponding, Genetics and Molecular Research, 2018, Genetics and Molecular Research, 17 (3), 18056, English

    [Refereed]

    Scientific journal

  • Honda T, Shibano M, Matsumoto H, Sasazaki S, Oyama K, Mannen H

    Sep. 2017, Animal Veterinary Sciences, 5, 69 - 72, English

    [Refereed]

    Scientific journal

  • Hideyuki Mannen, Riku Yonesaka, Aoi Noda, Takeshi Shimogiri, Ichiro Oshima, Kiyomi Katahira, Misao Kanemaki, Tetsuo Kunieda, Yousuke Inayoshi, Fumio Mukai, Shinji Sasazaki

    This study aims to estimate the mitochondrial genetic diversity and structure of Japanese Polled and Kuchinoshima feral cattle, which are maintained in small populations. We determined the mitochondrial DMA (mtDNA) displacement loop (D-loop) sequences for both cattle populations and analyzed these in conjunction with previously published data from Northeast Asian cattle populations. Our findings showed that Japanese native cattle have a predominant, Asian-specific mtDNA haplogroup T4 with high frequencies (0.43-0.81). This excluded Kuchinoshima cattle (32 animals), which had only one mtDNA haplotype belonging to the haplogroup T3. Japanese Polled showed relatively lower mtDNA diversity in the average sequence divergence (0.0020) than other Wagyu breeds (0.0036-0.0047). Japanese Polled have been maintained in a limited area of Yamaguchi, and the population size is now less than 200. Therefore, low mtDNA diversity in the Japanese Polled could be explained by the decreasing population size in the last three decades. We found low mtDNA diversity in both Japanese Polled and Kuchinoshima cattle. The genetic information obtained in this study will be useful for maintaining these populations and for understanding the origin of Japanese native cattle.

    WILEY, May 2017, ANIMAL SCIENCE JOURNAL, 88 (5), 739 - 744, English

    [Refereed]

    Scientific journal

  • Fuki Kawaguchi, Kazuki Okura, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Previous studies have indicated that some leptin gene polymorphisms were associated with economically important traits in cattle breeds. However, polymorphisms in the leptin gene have not been reported thus far in Japanese Black cattle. Here, we aimed to identify the leptin gene polymorphisms which are associated with carcass traits and fatty acid composition in Japanese Black cattle. We sequenced the full-length coding sequence of leptin gene for eight Japanese Black cattle. Sequence comparison revealed eight single nucleotide polymorphisms (SNPs). Three of these were predicted to cause amino acid substitutions: Y7F, R25C and A80V. Then, we genotyped these SNPs in two populations (JB1 with 560 animals and JB2 with 450 animals) and investigated the effects on the traits. Y7F in JB1 and A80V in JB2 were excluded from statistical analysis because the minor allele frequencies were low (<0.1). Association analysis revealed that Y7F had a significant effect on the dressed carcass weight in JB2; R25C had a significant effect on C18:0 and C14:1 in JB1 and JB2, respectively; and A80V had a significant effect on C16:0, C16:1, C18:1, monounsaturated fatty acid and saturated fatty acid in JB1. The results suggested that these SNPs could be used as an effective marker for the improvement of Japanese Black cattle.

    Corresponding, WILEY, Mar. 2017, ANIMAL SCIENCE JOURNAL, 88 (3), 433 - 438, English

    [Refereed]

    Scientific journal

  • Fuki KAWAGUCHI, Satoshi KOGA, Kenji OYAMA, Hideyuki MANNEN,, Shinji SASAZAKI

    Corresponding, Japanese Society of Animal Breeding and Genetics, 2017, The Journal of Animal Genetics, 45 (1), 3 - 8, Japanese

    [Refereed]

    Scientific journal

  • 齊藤祐也, 笹崎晋史, 下桐猛, 大島一郎, 片平清美, 印牧美佐生, 国枝哲夫, 万年英之

    Kuchinoshima cattle are unique feral cattle in Kuchinoshima Island in Kagoshima prefecture. We genotyped 54K SNPs for Kuchinoshima cattle, Japanese Black, Japanese Brown and Japanese Holstein using high density SNP chip to estimate genetic diversity and genetic structure in Kuchinoshima cattle. Average minor allele frequency in Kuchinoshima cattle was lower (0.089) than those of the other populations (0.181-0.251). Ratio of monomorphic SNPs for each chromosome in Kuchinoshima cattle ranged from 47.7% (BTA24) to 72.3% (BTA20). Subsequently, we investigated expected heterozygosity in each chromosome, and observed six monomorphic regions longer than 5Mb, especially, the longest region extended 12.80Mb. This study revealed the extremely low genetic diversity of Kuchinoshima cattle, due to founder effect derived from a few ancestral individuals and genetic drift caused by small population with below 100 animals lasting for a long time in Kuchinoshima Island.

    Japanese Society of Animal Science, Aug. 2016, 日本畜産学会報, 87 (3), 219 - 226, Japanese

    [Refereed]

    Scientific journal

  • Takahiro Nishimaki, Takayuki Ibi, Siqintuya, Naohiko Kobayashi, Tamako Matsuhashi, Takayuki Akiyama, Emi Yoshida, Kazumi Imai, Mayu Matsui, Keiichi Uemura, Hisayoshi Eto, Naoto Watanabe, Tatsuo Fujita, Yosuke Saito, Tomohiko Komatsu, Hiroshi Hoshiba, Hideyuki Mannen, Shinji Sasazaki, Tetsuo Kunieda

    Marker-assisted selection (MAS) is expected to accelerate the genetic improvement of Japanese Black cattle. However, verification of the effects of the genes for MAS in different subpopulations is required prior to the application of MAS. In this study, we investigated the allelic frequencies and genotypic effects for carcass traits of six genes, which can be used in MAS, in eight local subpopulations. These genes are SCD, FASN and SREBP1, which are associated with the fatty acid composition of meat, and NCAPG, MC1R and F11, which are associated with carcass weight, coat color and blood coagulation abnormality, respectively. The frequencies of desirable alleles of SCD and FASN were relatively high and that of NCAPG was relatively low, and NCAPG was significantly associated with several carcass traits, including carcass weight. The proportions of genotypic variance explained by NCAPG to phenotypic variance were 4.83 for carcass weight. We thus confirmed that NCAPG is a useful marker for selection of carcass traits in these subpopulations. In addition, we found that the desirable alleles of six genes showed no negative effects on carcass traits. Therefore, selection using these genes to improve target traits should not have negative impacts on carcass traits.

    WILEY, Apr. 2016, ANIMAL SCIENCE JOURNAL, 87 (4), 469 - 476, English

    [Refereed]

    Scientific journal

  • Riku Yonesaka, Shinji Sasazaki, Hiroshi Yasue, Satoru Niwata, Yousuke Inayoshi, Fumio Mukai, Hideyuki Mannen

    In this study, we genotyped 117 autosomal single nucleotide polymorphisms using a DigiTag2 assay to assess the genetic diversity, structure and relationships of 16 Eurasian cattle populations, including nine cattle breeds and seven native cattle. Phylogenetic and principal component analyses showed that Bos taurus and Bos indicus populations were clearly distinguished, whereas Japanese Shorthorn and Japanese Polled clustered with European populations. Furthermore, STRUCTURE analysis demonstrated the distinct separation between Bos taurus and Bos indicus (K=2), and between European and Asian populations (K=3). In addition, Japanese Holstein exhibited an admixture pattern with Asian and European cattle (K=3-5). Mongolian (K=13-16) and Japanese Black (K=14-16) populations exhibited admixture patterns with different ancestries. Bos indicus populations exhibited a uniform genetic structure at K=2-11, thereby suggesting that there are close genetic relationships among Bos indicus populations. However, the Bhutan and Bangladesh populations formed a cluster distinct from the other Bos indicus populations at K=12-16. In conclusion, our study could sufficiently explain the genetic construction of Asian cattle populations, including: (i) the close genetic relationships among Bos indicus populations; (ii) the genetic influences of European breeds on Japanese breeds; (iii) the genetic admixture in Japanese Holstein, Mongolian and Japanese Black cattle; and (iv) the genetic subpopulations in Southeast Asia.

    WILEY-BLACKWELL, Feb. 2016, ANIMAL SCIENCE JOURNAL, 87 (2), 190 - 196, English

    [Refereed]

    Scientific journal

  • Fuki KAWAGUCHI, Kenji FUKAZAWA, Namiko KOHAMA, Takayuki AKIYAMA, Moriyuki FUKUSHIMA, Eiji KOBAYASHI, Kenji OYAMA, Hideyuki MANNEN, Shinji SASAZAKI

    Corresponding, Japanese Society of Animal Science, 2016, Nihon Chikusan Gakkaiho, 87 (4), 333 - 338, Japanese

    [Refereed]

    Scientific journal

  • Kiri Hayakawa, Takayuki Sakamoto, Atsushi Ishii, Keita Yamaji, Yoshinobu Uemoto, Nanae Sasago, Eiji Kobayashi, Naohiko Kobayashi, Tamako Matsuhashi, Shin Maruyama, Hirokazu Matsumoto, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.

    Corresponding, WILEY-BLACKWELL, Aug. 2015, ANIMAL SCIENCE JOURNAL, 86 (8), 737 - 746, English

    [Refereed]

    Scientific journal

  • A. Waki, S. Sasazaki, E. Kobayashi, H. Mannen

    This study was a first analysis of paternal genetic diversity for extensive Asian domestic goats using SRY gene sequences. Sequencing comparison of the SRY 3-untranslated region among 210 Asian goats revealed four haplotypes (Y1A, Y1B, Y2A and Y2B) derived from four variable sites including a novel substitution detected in this study. In Asian goats, the predominant haplotype was Y1A (62%) and second most common was Y2B (30%). Interestingly, the Y2B was a unique East Asian Y chromosomal variant, which differentiates eastern and western Eurasian goats. The SRY geographic distribution in Myanmar and Cambodia indicated predominant the haplotype Y1A in plains areas and a high frequency of Y2B in mountain areas. The results suggest recent genetic infiltration of modern breeds into South-East Asian goats and an ancestral SRY Y2B haplotype in Asian native goats.

    WILEY-BLACKWELL, Jun. 2015, ANIMAL GENETICS, 46 (3), 337 - 339, English

    [Refereed]

    Scientific journal

  • Shinji Sasazaki, Kento Akiyama, Takahiro Narukami, Hirokazu Matsumoto, Kenji Oyama, Hideyuki Mannen

    Fatty acid composition of beef adipose tissue is one of its important traits because a high proportion of monounsaturated fatty acid is related to favorable beef flavor and tenderness. In this study, we searched polymorphisms in full length coding DNA sequence of urotensin 2 recepter and investigated the effects on fatty acid composition (C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, monounsaturated fatty acid, saturated fatty acid). Eight single nucleotide polymorphisms (SNP) were identified by sequence comparison among eight animals, including five Japanese Black and three Holstein cattle. One of these SNP (c.866C>T) was predicted to cause amino acid substitutions (P289L) and the other seven synonymous SNP, including c.267C>T, were presumed to be in linkage disequilibrium. Therefore we selected two SNP (c.267C>T and c.866C>T) for further analysis. We investigated associations between these genotypes and fatty acid composition in three Japanese Black populations (n=560, 245 and 287) and a Holstein population (n=202). Tukey-Kramer's honestly significant difference test revealed that CC genotype in c.267C>T indicated lower C14:0 and higher C18:1 than the other genotypes in Japanese Black cattle and CC genotype in c.866C>T showed lower C16:1 than CT genotype in Holstein cattle (P<0.05). These results suggested that these genotypes would contribute to production of high-grade meat as selection markers in beef cattle.

    Lead, WILEY-BLACKWELL, May 2014, ANIMAL SCIENCE JOURNAL, 85 (5), 499 - 505, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Takuya Nogi, Ichiro Tabuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    Genetic factors are known to influence meat quality in beef cattle. Expression of fatty acid desaturase 2 (FADS2) and fatty acid-binding protein 4 (FABP4) genes in the fat metabolism pathway has been reported to influence fat-related traits. In this study, we aimed to discover DNA polymorphisms in the promoter regions of the FADS2 and FABP4 genes in cattle populations to develop genetic markers. As a consequence, one single nucleotide polymorphism (SNP) in the FADS2 promoter (FADS2 g.-823G > A) and two linked SNPs in the FABP4 promoter (FABP4 g.-295A > G and FABP4 g.-287A > G) were identified. All of them were assumed to influence binding sites for transcription factors, suggesting these SNPs influence the genes' expression and beef quality. Therefore, we performed association analysis between the genotypes and traits. Our results revealed that the FADS2 g.-823G > A had significant effects on several traits including beef marbling score (BMS). BMS of the G/G animals in a Holstein population (2.36) was higher than that of the G/A animals (2.13). The same tendency was observed in Japanese Black populations. On the other hand, although the FABP4 SNPs also had significant effects on some traits, they did not show additive effects on any of the traits analyzed in this study. Our results suggest that the FADS2 g.-823G > A is a useful genetic marker for breeding in beef cattle. (C) 2014 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, May 2014, LIVESTOCK SCIENCE, 163, 34 - 40, English

    [Refereed]

    Scientific journal

  • A. Reverter, J. M. Henshall, R. McCulloch, S. Sasazaki, R. Hawken, S. A. Lehnert

    Pooled genomic DNA has been proposed as a cost-effective approach in genomewide association studies (GWAS). However, algorithms for genotype calling of biallelic SNP are not adequate with pooled DNA samples because they assume the presence of 2 fluorescent signals, 1 for each allele, and operate under the expectation that at most 2 copies of the variant allele can be found for any given SNP and DNA sample. We adapt analytical methodology from 2-channel gene expression microarray technology to SNP genotyping of pooled DNA samples. Using 5 datasets from beef cattle and broiler chicken of varying degrees of complexity in terms of design and phenotype, continuous and dichotomous, we show that both differential hybridization (M = green minus red intensity signal) and abundance (A = average of red and green intensities) provide useful information in the prediction of SNP allele frequencies. This is predominantly true when making inference about extreme SNP that are either nearly fixed or highly polymorphic. We propose the use of model-based clustering via mixtures of bivariate normal distributions as an optimal framework to capture the relationship between hybridization intensity and allele frequency from pooled DNA samples. The range of M and A values observed here are in agreement with those reported within the context of gene expression microarray and also with those from SNP array data within the context of analytical methodology for the identification of copy number variants. In particular, we confirm that highly polymorphic SNP yield a strong signal from both channels (red and green) while lowly or nonpolymorphic SNP yield a strong signal from 1 channel only. We further confirm that when the SNP allele frequencies are known, either because the individuals in the pools or from a closely related population are themselves genotyped, a multiple regression model with linear and quadratic components can be developed with high prediction accuracy. We conclude that when these approaches are applied to the estimation of allele frequencies, the resulting estimates allow for the development of cost-effective and reliable GWAS.

    AMER SOC ANIMAL SCIENCE, May 2014, JOURNAL OF ANIMAL SCIENCE, 92 (5), 1874 - 1885, English

    [Refereed]

    Scientific journal

  • M. R. S. Fortes, K. Kemper, S. Sasazaki, A. Reverter, J. E. Pryce, W. Barendse, R. Bunch, R. McCulloch, B. Harrison, S. Bolormaa, Y. D. Zhang, R. J. Hawken, M. E. Goddard, S. A. Lehnert

    A putative functional mutation (rs109231213) near PLAG1 (BTA14) associated with stature was studied in beef cattle. Data from 8199 Bos taurus, Bos indicus and Tropical Composite cattle were used to test the associations between rs109231213 and various phenotypes. Further, 23496 SNPs located on BTA14 were tested for association with these phenotypes, both independently and fitted together with rs109231213. The C allele of rs109231213 significantly increased hip height, weight, net food intake, age at puberty in males and females and decreased IGF-I concentration in blood and fat depth. When rs109231213 was fitted as a fixed effect in the model, there was an overall reduction in associations between other SNPs and these traits but some SNPs remained associated (P<10(-4)). Frequency of the mutant C allele of rs109231213 differed among B.indicus (0.52), B.taurus (0.96) and Tropical Composite (0.68). Most chromosomes carrying the C allele had the same surrounding 10 SNP haplotype, probably because the C allele was introgressed into Brahman from B.taurus cattle. A region of reduced heterozygosity surrounds the C allele; this is small in B.taurus but 20Mb long in Brahmans, indicating recent and strong selection for the mutant allele. Thus, the C allele appears to mark a mutation that has been selected almost to fixation in the B.taurus breeds studied here and introduced into Brahman cattle during grading up and selected to a frequency of 0.52 despite its negative effects on fertility.

    WILEY-BLACKWELL, Dec. 2013, ANIMAL GENETICS, 44 (6), 636 - 647, English

    [Refereed]

    Scientific journal

  • Effects of genes on economically important traits of Japanese Black cattle in Hyogo population

    Ohkura K, Akiyama T, YOSHIDA E, FUKUSHIMA M, IWAMOTO E, OKA A, MATSUMOTO H, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

    In this study, we investigated allelic frequencies and genetic effects of SCD, SREBP-1, FASN, EDG-1, F11, MC1R and CW-2 genes on beef carcass traits including fatty acid composition in the closed Hyogo population of Japanese Black cattle. The favorable allele frequencies were 0.960 in SCD, 0.322 in SREBP-1, 0.910 in FASN, 0.265 in EDG-1, 0.623 in F11, 0.464 in MC1R and 0.007 in

    Nihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84 (2), 157 - 162, Japanese

    [Refereed]

    Scientific journal

  • Mitochondrial genetic diversity of goat in South Eastern Asia

    Kato T, Takahashi A, Matsumoto H, SASAZAKI Shinji, FARUQUE O, MASANGKAY JS, Nomura K, Takahashi Y, Amano T, Yamamoto Y, Namikawa T, MANNEN Hideyuki

    In this study, we determined 481bp hypervariable sequence of mitochondrial DNA D-loop region for Bangladeshi and Philippine native goats. The mitochondrial genetic diversity was estimated using obtained sequences, and then the origins of predominance mitochondrial lineage A and B in South East Asia were investigated. The analyses of 53 Bangladesh and 30 Philippine goats reveale

    Nihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84 (2), 149 - 155, Japanese

    [Refereed]

    Scientific journal

  • Atsushi Ishii, Keita Yamaji, Yoshinobu Uemoto, Nanae Sasago, Eiji Kobayashi, Naohiko Kobayashi, Tamako Matsuhashi, Shin Maruyama, Hirokazu Matsumoto, Shinji Sasazaki, Hideyuki Mannen

    Fatty acid composition is one of the important traits in beef. The aim of this study was to identify candidate genomic regions for fatty acid composition by genome-wide association study with 50K single nucleotide polymorphism (SNP) array in Japanese Black cattle. A total of 461 individuals and 40657 SNPs were used in this study. We applied genome-wide rapid association using mixed model and regression (GRAMMAR) and genomic control approaches to estimate the associations between genotypes and fatty acid composition. In addition, two SNPs in fatty acid synthase (FASN) (T1952A) and stearoyl-CoA desaturase (SCD) (V293A) genes were also genotyped. Association analysis revealed that 30 significant SNPs for several fatty acids (C14:0, C14:1, C16:1 and C18:1) were located in the BTA19FASN gene located within this region but the FASN mutation had no significant effect on any traits. We also detected one significant SNP for C18:1 on BTA23 and two SNPs for C16:0 on BTA25. The region around 17Mb on BTA26 harbored two significant SNPs for C14:1 and SNP in SCD in this region showed the strongest association with C14:1. This study demonstrated novel candidate regions in BTA19, 23 and 25 for fatty acid composition.

    WILEY-BLACKWELL, Oct. 2013, ANIMAL SCIENCE JOURNAL, 84 (10), 675 - 682, English

    [Refereed]

    Scientific journal

  • Takahiro Nishimaki, Takayuki Ibi, Yoshihiro Tanabe, Yoshiyuki Miyazaki, Naohiko Kobayashi, Tamako Matsuhashi, Takayuki Akiyama, Emi Yoshida, Kazumi Imai, Mayu Matsui, Keiichi Uemura, Naoto Watanabe, Tatsuo Fujita, Yosuke Saito, Tomohiko Komatsu, Takahisa Yamada, Hideyuki Mannen, Shinji Sasazaki, Tetsuo Kunieda

    Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise F-ST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies.

    WILEY-BLACKWELL, Aug. 2013, ANIMAL SCIENCE JOURNAL, 84 (8), 585 - 591, English

    [Refereed]

    Scientific journal

  • Keita Yamaji, Daisuke Hosokawa, Atsushi Ishii, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    In our previous study, we examined divergently selected regions between Japanese Black cattle and Holstein cattle based on a 50k single nucleotide polymorphism (SNP) panel and a Sliding Window Allele Difference method resulting in the identification of 11 genomic regions. The aim of the present study was to investigate the association between these genomic regions and economic traits, including seven carcass and five milk production traits. For this purpose, representative SNP markers were selected from the 11 genomic regions and used to estimate the effects on the traits in Japanese Black cattle (N=488) and Holstein cattle (N=194). Association analyses revealed that five SNPs showed a significant effect on the carcass traits in Japanese Black cattle and other five SNP showed a significant association with milk production traits in Holstein cattle (P< 0.05). These results indicated that divergently selected regions identified using a Sliding Window Allele Difference method contain the quantitative trait loci (QTL) for economic traits. Furthermore, most of the QTL identified in this study were consistent with the previously reported QTL. Further investigations of these regions may lead to the identification of the genes and polymorphisms responsible for the economic traits. © 2013 Elsevier B.V.

    Elsevier, Aug. 2013, Livestock Science, 155 (2-3), 180 - 185, English

    [Refereed]

    Scientific journal

  • Bang Zhong Lin, Taiki Kato, Makoto Kaneda, Hirokazu Matsumoto, Shinji Sasazaki, Hideyuki Mannen

    In the current study, a total of 65 single nucleotide polymorphisms (SNPs) within the intron region were developed in goat (Capra hircus) by utilizing genomic information of cattle and sheep due to poor available genomic information on goat. Using these markers, we carried out genetic diversity and structure analyses for 10 Asian goat populations. The phylogenetic tree and principal components analysis showed good correspondence between clustered populations and their geographic locations. The STRUCTURE software analysis illustrated six divergent genetic structures among 10 populations. Myanmar and Cambodia populations showed high admixture patterns with different ancestry, suggesting genetic introgression into native goat populations. We also investigated the correlation between genetic diversity and geographic distance from a domestication center. This result showed a decreasing trend of genetic diversity according to the distance (P = 0.014). This result supported common consensus that western Asia is one of the centers of origin for modern Asian domestic goat.

    WILEY-BLACKWELL, Aug. 2013, ANIMAL SCIENCE JOURNAL, 84 (8), 579 - 584, English

    [Refereed]

    Scientific journal

  • Comparative analysis on gene expression profiles in longissimus dorsi muscle of Japanese Black cattle

    MATSUMOTO H, OGURI Y, MIZUNO Y, ISHIDA T, KOBAYASHI I, IWAISAKI H, SAITO K, SASAGO N, KOBAYASHI E, KUGE S, HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

    Recently, fatty acid composition has been recognized as an important economic trait of Japanese Black cattle. The aim of this study was to seek for candidate genes which might be useful for beef breeding to increase the mono-unsaturated fatty acid (MUFA) level. For this purpose, we compared gene expression profile of Japanese Black cattle which showed high percentage of MUFA in

    日本動物遺伝育種学会, Jun. 2013, The Journal of Animal Genetics, 41 (1), 7 - 14, Japanese

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Yumiko Shimizu, Atsuko Tanaka, Takuya Nogi, Ichiro Tabuchi, Kenji Oyama, Masaaki Taniguchi, Hideyuki Mannen, Shinji Sasazaki

    Genetic analyses have contributed to improvements of economically important traits derived from adipose tissue such as fatty acid composition in beef. Elongation of very long chain fatty acids (ELOVL) genes encode for the enzymes that play an important role in elongation of long-chain fatty acids. In this study, we aimed to discover genetic polymorphisms of ELOVL gene family in cattle populations to develop genetic markers. As a result, five synonymous mutations were detected in the coding regions of the ELOVL1, ELOVL2, ELOVL3 and ELOVL5 genes. In addition, six mutations were identified in promoter region of the ELOVL5. Two of five mutations in the promoter region of ELOVL5 were expected to alter the ELOVL5 expression and influence the economic traits, because of the high synteny of the region which was essential for activation of Elovl5 in mouse. Therefore, we performed association analysis between the genotypes and traits and our result revealed that T allele of g.-110T > C in ELOVL5 gene promoter indicated significantly thinner subcutaneous fat thickness (TT, 2.39 cm; CT, 2.35 cm) than that of C allele (CC, 2.68 cm) in a Japanese Black population. Our results suggest that the g.-110T > C is a useful genetic marker for the breeding in beef cattle.

    SPRINGER, Apr. 2013, MOLECULAR BIOLOGY REPORTS, 40 (4), 3231 - 3237, English

    [Refereed]

    Scientific journal

  • B. Z. Lin, S. Odahara, M. Ishida, T. Kato, S. Sasazaki, K. Nozawa, H. Mannen

    The domestic goat is one of the most important livestock species, but its origins and genetic diversity still remain uncertain. Multiple highly divergent maternal lineages of goat have been reported in previous studies. Although one of the mitochondrial DNA lineages, lineage B, was detected only in eastern and southern Asia, the geographic distribution of these lineages was previously unclear. Here, we examine the genetic diversity and phylogeographic structure of Asian goats by mitochondrial DNA sequences and morphological characteristics. The analyses of a total of 1661 Asian goats from 12 countries revealed a high frequency of lineage B in Southeast Asia. The frequency of this lineage tended to be higher in mountain areas than in plain areas in Southeast Asian countries, and there was a significant correlation between its frequency and morphological traits. The results suggest an original predominance of lineage B in Southeast Asia and the recent infiltration of lineage A into Southeast Asian goats.

    WILEY-BLACKWELL, Feb. 2013, ANIMAL GENETICS, 44 (1), 79 - 85, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Kenta Sasaki, Takuya Bessho, Eiji Kobayashi, Tsuyoshi Abe, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

    Fatty acid composition is an important economic trait for both dairy and beef cattle and controlled by genetic factors. Candidate genes controlling fatty acid composition may be found in fat synthesis and metabolism pathways. Acetyl-CoA carboxylase is the flux-determining enzyme in the regulation of fatty acid synthesis in animal tissues. One of two isozymes of this enzyme, acetyl-CoA carboxylase-alpha (ACACA), catalyses the first committed step of fatty acid synthesis in mammalian cytosol, leading to the biosynthesis of long-chain fatty acids. In the current study, the sequence comparison of the coding sequence (CDS) and two promoter regions (PIA and PIII) in bovine ACACA gene was performed between Japanese Black and Holstein cattle to detect nucleotide polymorphisms influencing fatty acid composition in milk and beef. Five single nucleotide polymorphisms (SNPs) were identified in the CDS region, 28 SNPs in the PIA region and three SNPs in the PIII region. Association study revealed that CCT/CCT type of PIII_#1, #2/PIA_#26 indicated a higher percentage of C14:0 in the milk of the Holstein cattle than CCT/GTC type (p = 0.050) and that a difference of the percentage of C16:0 was observed between CCT/CCT and GTC/GTC type (p = 0.023). CDS_#2 T/T type indicated a higher percentage of C18:0 than T/C type (p = 0.008). In addition, the Japanese Black cattle with CC/GT type of PIII_#1, #2 showed a higher percentage of C18:2 in the meat than those with GT/GT type (p = 0.025). Since PIII is the promoter specific to mammary gland during lactation, the altered expression of the ACACA gene owing to the SNPs in the PIII region may influence the fatty acid composition in the milk.

    SPRINGER, Sep. 2012, MOLECULAR BIOLOGY REPORTS, 39 (9), 8637 - 8644, English

    [Refereed]

    Scientific journal

  • Takeshi Honda, Shinji Sasazaki, Kenji Oyama, Fumio Mukai, Tetsuro Nomura

    The estimated allele frequency at neutral DNA marker loci is a fundamental parameter for establishing a conservation scheme for a set of livestock breeds. In this study, we propose a novel minimum distance (MD) method for estimating neutral allele frequencies, which minimizes the error by the use of pedigree information. Using computer simulation and actual microsatellite data for a pedigreed cattle population, the performance of the proposed method was compared with that of conventional random sampling (RND). MD manifests a promising superiority to RND, irrespective of the sample size and the depth of pedigree. It is theoretically demonstrated that the error of the estimates depends on both the sample size and the distance of founder allele frequencies between the sampled and the non-sampled individuals. MD samples individuals so as to minimize the latter source of estimation error.

    WILEY-BLACKWELL, Jun. 2012, JOURNAL OF ANIMAL BREEDING AND GENETICS, 129 (3), 226 - 233, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Satoshi Inada, Eiji Kobayashi, Tsuyoshi Abe, Hiroyuki Hasebe, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

    FASN is a multifunctional enzyme that catalyzes de novo biosynthesis of long-chain saturated fatty acid, and alteration of this enzyme may affect economic traits including fatty acid composition. Sequencing the coding sequence (CDS) of the FASN gene has so far been done only partially for dairy cattle. In this study, the CDS of the FASN gene of eight Holstein cattle was sequenced to detect nucleotide polymorphisms. We could identify 13 single nucleotide polymorphisms (SNPs) of which five were non-synonymous mutations (H1390Y, I1856L, T1950A, W1955R and T2264A). The association between these SNPs and economic traits was analyzed using 198 Holstein dams. Genotypes of H1390Y corresponded to those of the I1856L in the group, suggesting these SNPs were linked. Furthermore, the T1950A was suggestively linked with the W1955R. The association study revealed that T1950A/W1955R had an effect on milk fat content and the C14 Index. This will help to understand the complex association between the FASN gene and economic traits. (C) 2011 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, Apr. 2012, LIVESTOCK SCIENCE, 144 (3), 281 - 284, English

    [Refereed]

    Scientific journal

  • Daisuke Hosokawa, Atsushi Ishii, Keita Yamaji, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

    Differences between average allelic frequencies of genes that relate to traits suggest that it would be evidence of artificial selections. Sliding window approach is a useful method to identify genomic regions that have been differently selected between two breeds. The objective of this study was to identify the divergently selected regions between Japanese Black (JB) and Japanese Holstein (JH) cattle based on genotypic information obtained through a high-density single nucleotide polymorphism (SNP) panel. After genotyping of 54 001 SNP markers on 100 animals (50 JB and 50 JH), 40 635 SNPs were suitable for the analysis. For each of these SNPs, the absolute difference between allelic frequencies of JB and JH was calculated. In the current study, 10 consecutive SNPs were defined as components of a window. For each window, the average difference in allelic frequency was calculated. This was termed sliding window average difference (SWAD). Among 40 055 windows, we focused on 39 windows with the largest SWAD. This was equivalent to 0.1% of all windows and the SWAD was more than 0.435. Some of these windows overlapped and were distributed in 11 regions. These regions were in good agreement with reported quantitative trait locus, therefore would be selection signatures and good candidates that harbor the causative mutations.

    WILEY-BLACKWELL, 2012, ANIMAL SCIENCE JOURNAL, 83 (1), 7 - 13, English

    [Refereed]

    Scientific journal

  • 黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果

    鳴神貴太, MANNEN HIDEYUKI, OYAMA KENJI, 庄司則章, 中嶋宏明, SASAZAKI SHINJI

    日本畜産学会, Nov. 2011, 日本畜産学会報, 82 (2), 125 - 130, Japanese

    [Refereed]

    Scientific journal

  • Shinji Sasazaki, Daisuke Hosokawa, Rie Ishihara, Hiroki Aihara, Kenji Oyama, Hideyuki Mannen

    In the meat industry, correct labeling of beef origins or breed is required to assure quality and safety. This paper describes the development of discrimination markers between Japanese domestic and imported beef from the United States (US) and Australia (AUS) based on a bovine 50K single nucleotide polymorphism (SNP) array using a total of 110 samples: Japanese Black (n = 50), Japanese Holstein (n = 50) and US cattle (n = 10). Genotyping information revealed 1081 SNPs as candidate markers that were polymorphic only in US cattle. The genotyping results by PCR - restriction length polymorphism in Japanese Black (n = 300) and Holstein cattle (n = 146) revealed that 11 SNPs had alleles specific to US cattle. Their allelic frequencies in US cattle (n = 108) ranged from 0.097 to 0.250 with an average of 0.178 and the combined identification probability of US cattle was 0.987. In addition, we also verified the applicability of these US-specific markers to AUS cattle. Their allelic frequencies in AUS cattle (n = 280) ranged from 0.063 to 0.224 with an average of 0.137 and the combined identification probability of AUS cattle was 0.963. In conclusion, a set of these markers could be useful for discriminating between Japanese domestic and imported beef and would contribute to identify origins and prevent falsified labeling of beef.

    WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82 (1), 67 - 72, English

    [Refereed]

    Scientific journal

  • Takahiro Narukami, Shinji Sasazaki, Kenji Oyama, Takuya Nogi, Masaaki Taniguchi, Hideyuki Mannen

    Fatty acid composition of adipose tissue has been recognized as an important carcass trait because of its relationship with eating quality such as favorable beef flavor and tenderness. Therefore, we investigated the effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) on fatty acid composition in intramuscular fat tissue of Holstein steers. The major allele frequencies were 0.705 in SCD, 0.518 in FABP4, 0.888 in FASN, and 0.984 in LXR. Genotyping of SCD showed significant effect on C14:0, C14:1, C18:0 and saturated fatty acid (P < 0.05). In addition, the result suggested that SCD genotype possibly had effect on composition of C18:1 and monounsaturated fatty acid. Genotype of FABP4 had significant effect on composition of C16:0. Effect of LXR genotypes could not be analyze because of extremely biased genotype frequencies. Our results suggest that genotypes of SCD and FABP4 may in part affect meat quality in Holstein.

    WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82 (3), 406 - 411, English

    [Refereed]

    Scientific journal

  • Makoto Kaneda, Bang Zhong Lin, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

    Allele frequencies of 10 representative polymorphisms for beef and milk traits were investigated for a total of 240 animals from Bos taurus and Bos indicus breeds, including two Japanese groups (Japanese Black and Japanese Brown), two East Asian groups (Korean and Mongolian), three European groups (Holstein, Angus and Hereford) and a Bos indicus group in South Asia (Myanmar, Laos and Cambodia). The Japanese Black revealed unique genetic construction in GH, FASN and SREBP-1 and the other Asian populations show intermediate frequencies between European and Japanese populations. The Bos indicus group showed low favorable allele frequencies in most of the genes. The study showed the variability and distribution of 10 genes affecting economic traits among world representative cattle breeds. The genetic information would contribute to elucidating the genetic background for worldwide cattle breeds and the possibility of improvement using the markers.

    WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82 (6), 717 - 721, English

    [Refereed]

    Scientific journal

  • Suppression of WWP1 Gene Via RNAi Induced the Reduction of Proliferation Rate of C2C12 Myoblasts

    Hirokazu Matsumoto, Michihiro Takahama, Ryohsuke Kajiyama, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

    The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), which plays an important role in ubiquitin-proteasome pathway to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Previously, we transfected either of wild and mutated types of WWP1 gene into C2C12 mouse myoblasts to monitor the expression pattern of muscle-differentiation markers, so that excessive WWP1 expression enhanced the expression of the myosin heavy chain (MyHC) Ia gene but lowered the expression of the MyHC IIb gene, while mutated WWP1 gene transfected in to myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. However, the mechanism for the mutation to inhibit muscle differentiation remains elucidated. The current study attempted to suppress the WWP1 expression by RNAi technique and to observe its effect on C2C12 cells. The effect of WWP1 suppression was clearly different from that of the R441Q missense mutation in the WWP1 gene. The WWP1 suppression reduced the proliferation rate of C2C12 myoblasts, while clear difference was not observed in the proliferation rate by the transfection of the mutated WWP1 gene into the cells. Our RT-PCR analysis indicated that the reduction of the WWP1 expression is the specific effect mediated by RNAi and that the reduction of proliferation rate observed in this study is largely attributed to the suppression of the WWP1 expression. These data indicated that the mutation responsible for chicken muscular dystrophy does not eliminate the enzymatic activity and provides some new function for the gene.

    JAPAN POULTRY SCIENCE ASSOC, Oct. 2010, JOURNAL OF POULTRY SCIENCE, 47 (4), 288 - 293, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Shinji Sasazaki, Akira Fujiwara, Nobutsune Ichihara, Tateki Kikuchi, Hideyuki Mannen

    Members of the caveolin family are the main component of caveolae, and caveolin-3 is a muscle-specific protein. Caveolin-3 deficiency induces a muscular dystrophic phenotype, while its overexpression is also harmful to muscle cells Increased caveolae were observed in chicken muscular dystrophy, however, the underlying mechanism causing the onset remains unclear Therefore, the current study analyzes the expression of caveolin-3 and other caveola-related proteins in dystrophic chickens. Western blotting and semi-quantitative RT-PCR analysis revealed that (1) caveolin-3 is highly expressed in the damaged muscle of dystrophic chickens (7 12-fold), (2) the amount of caveolin-3 protein is regulated in posttranslational modification, since no significant increase is observed at the mRNA level (1.09-fold), and (3) the expression pattern of other caveola-related proteins is similar to that of caveolin-3 These results suggest that the accumulation of caveolin-3 protein may be associated with the causative process of chicken muscular dystrophy. (C) 2010 Elsevier Inc. All rights reserved

    ELSEVIER SCIENCE INC, Sep. 2010, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 157 (1), 68 - 72, English

    [Refereed]

    Scientific journal

  • Mitochondrial DNA variation and genetic construction of indigenous cattle population in Bhutan

    Dorji T, Kaneda M, Lin BZ, Takahashi A, OYAMA Kenji, SASAZAKI Shinji, Yamamoto Y, Kawamoto Y, MANNEN Hideyuki

    Japanese Society of Animal Genetics, Jun. 2010, Journal of Animal Genetics, 38 (1), 77 - 81, English

    [Refereed]

    Scientific journal

  • Y. Suekawa, H. Aihara, M. Araki, D. Hosokawa, H. Mannen, S. Sasazaki

    In Japan, Japanese Black and Holstein cattle are appreciated as popular sources of meat, and imported beef from Australia and the United States is also in demand in the meat industry. Since the BSE outbreak, the problem of false sales has arisen: imported beef has sometimes been mislabeled as domestic beef due to consumer concerns. A method is needed to correctly discriminate between Japanese and imported cattle for food safety. The objective of this study was to develop breed discrimination markers between Japanese and US cattle using a 50K SNP array. As a result, five US-specific markers (BISNP7, BISNP15, BISNP21, BISNP23, and BISNP26) were developed with allelic frequencies that ranged from 0.102 (BISNP15) to 0.250 (BISNP7) and averaged 0.184. The combined use of the five markers would permit discrimination between Japanese and US cattle with a probability of identification of 0.858. This result indicates the potential of the bovine 50K SNP array as a powerful tool for developing breed identification markers. These markers would contribute to the prevention of falsified beef displays in Japan. (C) 2010 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, Jun. 2010, MEAT SCIENCE, 85 (2), 285 - 288, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Yumi Inba, Shinji Sasazaki, Akira Fujiwara, Nobutsune Ichihara, Tateki Kikuchi, Hideyuki Mannen

    The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), an enzyme to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite of intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Since it is generally known that the switching of myosin heavy chain (MyHC) isoforms from neonatal isoform to adult one is inhibited in chicken muscular dystrophy, we transfected either of wild and mutated types of WWP1 gene into C2C12 cells to monitor the expression pattern of muscle-differentiation markers including MyHCs by real-time PCR. Excessive WWP1 expression enhanced the expression of the MyHC la gene but lowered the expression of the MyHC IIb gene. On the other hand, mutated WWP1 gene transfected into myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. The present data suggest that WWP1 promotes myoblast differentiation from embryonic into fast twitch phase while a mutation in WWP1 results to retain slow and fast twitch isoforms characteristic of dystrophic fast twitch muscles.

    JAPAN POULTRY SCIENCE ASSOC, Apr. 2010, JOURNAL OF POULTRY SCIENCE, 47 (2), 115 - 119, English

    [Refereed]

    Scientific journal

  • Shanyuan Chen, Bang-Zhong Lin, Mumtaz Baig, Bikash Mitra, Ricardo J. Lopes, Antonio M. Santos, David A. Magee, Marisa Azevedo, Pedro Tarroso, Shinji Sasazaki, Stephane Ostrowski, Osman Mahgoub, Tapas K. Chaudhuri, Ya-ping Zhang, Vania Costa, Luis J. Royo, Felix Goyache, Gordon Luikart, Nicole Boivin, Dorian Q. Fuller, Hideyuki Mannen, Daniel G. Bradley, Albano Beja-Pereira

    Animal domestication was a major step forward in human prehistory, contributing to the emergence of more complex societies. At the time of the Neolithic transition, zebu cattle (Bos indicus) were probably the most abundant and important domestic livestock species in Southern Asia. Although archaeological evidence points toward the domestication of zebu cattle within the Indian subcontinent, the exact geographic origins and phylogenetic history of zebu cattle remains uncertain. Here, we report evidence from 844 zebu mitochondrial DNA (mtDNA) sequences surveyed from 19 Asiatic countries comprising 8 regional groups, which identify 2 distinct mitochondrial haplogroups, termed I1 and I2. The marked increase in nucleotide diversity (P < 0.001) for both the I1 and I2 haplogroups within the northern part of the Indian subcontinent is consistent with an origin for all domestic zebu in this area. For haplogroup I1, genetic diversity was highest within the Indus Valley among the three hypothesized domestication centers (Indus Valley, Ganges, and South India). These data support the Indus Valley as the most likely center of origin for the I1 haplogroup and a primary center of zebu domestication. However, for the I2 haplogroup, a complex pattern of diversity is detected, preventing the unambiguous pinpointing of the exact place of origin for this zebu maternal lineage. Our findings are discussed with respect to the archaeological record for zebu domestication within the Indian subcontinent.

    OXFORD UNIV PRESS, Jan. 2010, MOLECULAR BIOLOGY AND EVOLUTION, 27 (1), 1 - 6, English

    [Refereed]

    Scientific journal

  • Tashi Dorji, Han Jianlin, Po Wafula, Yoshio Yamamoto, Shinji Sasazaki, Kenji Oyama, Oliver Hanotte, Bang-Zhong Lin, Hideyuki Mannen

    Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.

    WILEY-BLACKWELL PUBLISHING, INC, 2010, ANIMAL SCIENCE JOURNAL, 81 (2), 145 - 151, English

    [Refereed]

    Scientific journal

  • Kazuhiro Hara, Hideki Watabe, Shinji Sasazaki, Fumio Mukai, Hideyuki Mannen

    This study describes the development of efficient single nucleotide polymorphism (SNP) markers for individual identification and parentage tests in a Japanese Black cattle population. An amplified fragment length polymorphism method was employed to detect informative candidate markers, and yielded 44 SNP markers from 220 primer combinations. 29 unlinked SNPs were finally selected as diagnostic markers. The allelic frequencies for each marker were estimated by using PCR-RFLP in the Japanese Black population. Based on the frequency data, the estimated identity power of these markers was 2.73 x 10-12. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.96929 and 0.99693, respectively. This panel of SNP markers is theoretically sufficient for individual identification, and would also be a powerful tool for a parentage test in Japanese Black cattle. The markers could contribute to the management of the beef industry in Japan.

    WILEY-BLACKWELL PUBLISHING, INC, 2010, ANIMAL SCIENCE JOURNAL, 81 (2), 152 - 157, English

    [Refereed]

    Scientific journal

  • Bang Zhong Lin, Shinji Sasazaki, Hideyuki Mannen

    The purpose of this study was to assess genetic diversity, phylogenetic relationship and population structure among nine Eurasian cattle populations using 58 single nucleotide polymorphism (SNP) markers. The calculated distribution of minor allele frequencies and heterozygosities suggested that the genetic diversity of Bos indicus populations was lower than that of Bos taurus populations. Phylogenetic analyses revealed the main divergence between the Bos taurus and Bos indicus populations, and subsequently between Asian and European populations. By principal components analysis, the Bos taurus and Bos indicus populations were clearly distinguished with PC1 (61.1%); however, six Bos taurus populations clustered loosely and the partial separation between European and Asian groups was observed by PC2 (12.5%). The structure analysis was performed using the STRUCTURE program. Distinct separation between Bos taurus and Bos indicus was shown at K = 2, and that between European and Asian populations at K = 3. At K = 4, 5 and 6, Mongolian population showed an admixture pattern with different ancestry of Asian and European cattle. At K = 7, all Bos taurus populations showed each cluster with little proportion of admixture. In conclusion, 58 SNP markers in this study could sufficiently estimate the genetic diversity, relationship and structure for nine Eurasian cattle populations, especially by analyses of principal components and STRUCTURE.

    WILEY-BLACKWELL, 2010, ANIMAL SCIENCE JOURNAL, 81 (3), 281 - 289, English

    [Refereed]

    Scientific journal

  • Kazuhiro Hara, Yukari Kon, Shinji Sasazaki, Fumio Mukai, Hideyuki Mannen

    Individual identification and parentage analysis using DNA markers are essential for assuring food identity and managing livestock population. The objective of this study was to develop a single nucleotide polymorphism (SNP) panel system for individual effective identification and parentage testing in a Japanese Black cattle population using BovineSNP50 BeadChip. On the basis of SNP frequencies, 60 unlinked informative SNPs were finally selected as candidate markers. The allelic frequencies for each SNP were estimated using additional individuals by PCR-RFLP (restriction fragment length polymorphism). A total of 87 SNP markers added in conjunction with previously developed 27 SNPs were evaluated to assess the utility of the test. The estimated identity power was 2.01 x 10-34. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.99999997 and 0.99998010, respectively. This developed SNP panel was quite powerful and could successfully exclude false sires with a probability of > 0.9999 even if the dam's genotype information was not obtained. The SNP system would contribute to management of the beef industry in Japan.

    WILEY-BLACKWELL, 2010, ANIMAL SCIENCE JOURNAL, 81 (4), 506 - 512, English

    [Refereed]

    Scientific journal

  • SASAZAKI Shinji, Usui M, Yoshozaki Y, Taniguchi M, Hasebe H, Abe T, Kobayashi E, MANNEN Hideyuki

    MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F2 Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.

    Japanese Society of Animal Genetics, Jun. 2009, Journal of Animal Genetics, 37 (1), 3 - 8, English

    [Refereed]

    Scientific journal

  • SASAZAKI Shinji, Usui M, Yoshozaki Y, Taniguchi M, Hasebe H, Abe T, Kobayashi E, MANNEN Hideyuki

    MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F2 Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.

    Japanese Society of Animal Genetics, Jun. 2009, Journal of Animal Genetics, 37 (1), 3 - 8, English

    [Refereed]

    Scientific journal

  • Hideki Ohsaki, Atsuko Tanaka, Shogo Hoashi, Shinji Sasazaki, Kenji Oyama, Masaaki Taniguchi, Fumio Mukai, Hideyuki Mannen

    Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle.

    WILEY-BLACKWELL PUBLISHING, INC, Jun. 2009, ANIMAL SCIENCE JOURNAL, 80 (3), 225 - 232, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Hideaki Maruse, Shinji Sasazaki, Akira Ftijiwara, Shin'ichi Takeda, Nobutsune Ichihara, Tateki Kikuchi, Fumio Mukai, Hideyuki Mannen

    The WW domain containing E3 ubiquitin protein ligase 1 (WWP1) is classified into one of ubiquitin ligases which play an important role in ubiquitin-proteasome pathway. Previously, we identified the WWP1 gene as a candidate gene of chicken Muscular dystrophy by linkage analysis and sequence comparison. However, the mechanism causing pathological changes and underlaying gene function remains elucidated. In the present study. we analyzed the WWP1 gene expression in various Muscles and tissues of normal chickens, and compared with those Front muscular dystrophic chickens. Two mRNA isoforms were detected in all tissues examined and revealed almost equal expression level. The WWP1 expression of dystrophic chickens was decreased in almost all skeletal Muscles including unaffected Muscles. These data indicate that there might not be a causal relationship between the alteration of WWP1 expression level and the severity of muscular dystrophy.

    JAPAN POULTRY SCIENCE ASSOC, Apr. 2009, JOURNAL OF POULTRY SCIENCE, 46 (2), 95 - 99, English

    [Refereed]

    Scientific journal

  • Bang Zhong Lin, Shinji Sasazaki, June Heon Lee, Hideyuki Mannen

    Growth hormone receptor (GHR) belongs to a member of the cytokine receptor superfamily. Polymorphism of presence or absence of an approximately 1.2 kbp LINE-1 element is observed in bovine GHR gene. The present study was carried out for estimating the genetic diversity and the origin of the LINE-1 element in 10 European, Southeastern Asian and East Asian cattle breeds or populations. Genotyping of the LINE-1 revealed predominant LINE-1 presence in European breeds (0.917 similar to 0.991), absence in the Bos taurus indicus populations (0.000 similar to 0.017), and intermediate presence in Northeast Asian cattle (0.417 similar to 0.522). From genetic features of LINE families, LINE-1 of GHR could be attributed to the same origin in both European and Asian cattle, and Asian LINE-1 may not be derived from recent introgression. This result suggested that LINE-1 in bovine GHR gene could have arisen in an ancestral population of Bos taurus taurus.

    WILEY, 2009, ANIMAL SCIENCE JOURNAL, 80 (5), 528 - 531, English

    [Refereed]

    Scientific journal

  • Shogo Hoashi, Tomoko Hinenoya, Atsuko Tanaka, Hideki Ohsaki, Shinji Sasazaki, Masaaki Taniguchi, Kenji Oyama, Fumio Mukai, Hideyuki Mannen

    Background: Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding protein (FABP4), liver X receptor alpha (LXR alpha), cytochrome b(5) (Cyt b(5)), long-chain acyl-CoA synthetase (ACSL) 1, ACSL4 and diacylglycerol acyltransferase 2 (DGAT2) and fatty acid composition. Results: Sequence comparisons revealed 14 single nucleotide polymorphisms in six genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXR alpha, were nonsynonymous substitutions. The associations between the genotypes and fatty acid compositions were analyzed by using 234 Japanese Black cattle. The genotypes of FABP4 I74V and LXR alpha V133I were significantly associated with palmitoleic acids (C16: 1, P = 0.0086) and linoleic acid (C18:2, P = 0.0121) content in intramuscular fat, respectively. Conclusion: Our findings suggest that the two polymorphisms of FABP4 I74V and LXR alpha V133I might be genetic factors in part associated with palmitoleic acid (FABP4 I74V) and linoleic acid (LXR alpha V133I) composition in intramuscular fat of Japanese Black cattle, respectively. Especially, FABP4 I74V had highly significant effect (P < 0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.

    BIOMED CENTRAL LTD, Dec. 2008, BMC GENETICS, 9, 84, English

    [Refereed]

    Scientific journal

  • Shinji Sasazaki, T. Nagato, H. Mannen

    This study seeks to investigate the genetic variability of PRNP in Asian goats. We sequenced the PRNP coding region using a total of 193 samples from seven Asian countries (Japan, Laos, Vietnam. Bhutan, Mongolia. Myanmar and Cambodia). Sequence comparison revealed five previously reported polymorphisms in the PRNP coding region. Two of those polymorphisms (G126A and C414T) were silent mutations, and the other three (T304G, A428G and T718C) Caused amino acid changes (W102G, H143R and S240P). In the total of 193 animals, one amino acid mutation (T304G) exhibited low variability (minor allele frequency = 0.04), but the other four were high (0.31-0.36). In addition, allele frequencies of C414T and T718C exhibited remarkable differences among countries (p-values of 6.50E-17 and 5.49E-18). These results Suggest high genetic variability of PRNP among these countries and are useful information for estimating genetic diversity in Asian goats. (C) 2008 Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, Nov. 2008, SMALL RUMINANT RESEARCH, 80 (1-3), 101 - 103, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Hideaki Maruse, Yumi Inaba, Kanako Yoshizawa, Shinji Sasazaki, Akira Fujiwara, Masahide Nishibori, Akinori Nakamura, Shin'ichi Takeda, Nobutsune Ichihara, Tateki Kikuchi, Fumio Mukai, Hideyuki Mannen

    Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1 Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence comparison and gene expression analysis to elucidate the responsible gene. One missense mutation was detected in AM candidate genes, while no remarkable alteration of expression patterns was observed. The mutation was identified in WWP1, detected only in dystrophic chickens within several tetrapods. These results suggested WWP1 is responsible for chicken muscular dystrophy. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    ELSEVIER SCIENCE BV, Jun. 2008, FEBS LETTERS, 582 (15), 2212 - 2218, English

    [Refereed]

    Scientific journal

  • Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genes

    D. Yoon, Y. S. Kwon, K. Y. Lee, W. Y. Jung, S. Sasazaki, H. Mannen, J. T. Jeon, J. H. Lee

    In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and a 528 bp fragment of ND5 gene in the bovine mtDNA were amplified by PCR and digested with three restriction enzymes, Msel, HpyCH(4)III and Tsp509I, respectively. The results showed that Bos taurus (T) type was the majority in Hanwoo by combining three markers (99.5%). However, 78.2% of Bos indicus (1) type was observed in the imported beef samples. These results indicated that three markers used in this study will be used as valuable markers for discriminating imported beef against Hanwoo.

    ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Jan. 2008, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 21 (1), 25 - 28, English

    [Refereed]

    Scientific journal

  • Yumi Inaba, Kei Tsurifune, Shinji Sasazaki, Hideyuki Mannen

    In this study, we describe intraspecies variation in the alpha C connector region of the bovine fibrinogen A alpha gene. Sequencing and genotyping of six bovine breeds revealed 7 to 10 tandem repeats in the alpha C connector region. In addition, we observed length differences between B. indicus and B. taurus, with the B. indicus having longer fibrinogen alpha C connectors (10-repeat alleles) than B. taurus (7- and 9-repeats). The difference in tandem repeats may be related to the function of blood coagulation system.

    TAYLOR & FRANCIS INC, 2008, ANIMAL BIOTECHNOLOGY, 19 (2), 122 - 126, English

    [Refereed]

    Scientific journal

  • Shogo Hoashi, Nobuhisa Ashida, Hideki Ohsaki, Takeshi Utsugi, Shinji Sasazaki, Masaaki Taniguchi, Kenji Oyama, Fumio Mukai, Hideyuki Mannen

    To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.

    SPRINGER, Dec. 2007, MAMMALIAN GENOME, 18 (12), 880 - 886, English

    [Refereed]

    Scientific journal

  • H. Ohsaki, T. Sawa, S. Sasazaki, K. Kano, M. Taniguchi, F. Mukai, H. Mannen

    Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of monounsaturated fatty acids (MUFAs). In cattle, the MUFAs are related to softness and flavor of meat. In order to investigate gene expression profile during bovine preadipocyte differentiation, we isolated stromal-vascular cells from perirenal adipose tissues of Japanese Black and Holstein steers. Gene expression level of adipocyte type fatty acid binding protein (FABP4), SCD, sterol regulatory element binding protein 1 (SREBP1) and CCAAT/enhancer binding protein alpha (C/EBP-alpha) were elucidated by real-time PCR assay. The levels of SCD mRNA expression were significantly increased to 10.8 and 6.3-fold in Japanese Black and Holstein, respectively, on day 1 of the culture. The difference in SCD expression between the two breeds may reflect differences in the fat development characteristics of the cattle breeds. Although transcription factors SREBP1 and C/EBP-alpha are supposed to regulate SCD expression, expression levels of the two factors were not completely consistent with that of SCD. (c) 2007 Elsevier Inc. All rights reserved.

    ELSEVIER SCIENCE INC, Nov. 2007, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 148 (3), 629 - 634, English

    [Refereed]

    Scientific journal

  • Hirokazu Matsumoto, Hideaki Maruse, Kanako Yoshizawa, Shinji Sasazaki, Akira Fujiwara, Takeki Kikuchi, Nobutsune Ichihara, Fumio Mukai, Hideyuki Mannen

    Muscular dystrophies, a group of inherited diseases with the progressive weakness and degeneration of skeletal muscle, contain genetically variable diseases. Though chicken muscular dystrophy with abnormal muscle (AM) has long been known, the gene responsible has not yet been identified. In this study, a resource family for AM was established with 487 F2 individuals and 22 gene markers, including microsatellite and insertion-deletion markers, were developed. The haplotypes were analyzed with these markers for the candidate region of GGA2q described in a previous study. The candidate region was successfully narrowed down to approximately 1Mbp. The region included seven functional genes predicted as the most likely AM candidates.

    BLACKWELL PUBLISHING, Oct. 2007, ANIMAL SCIENCE JOURNAL, 78 (5), 476 - 483, English

    [Refereed]

    Scientific journal

  • S. Sasazaki, H. Mutoh, K. Tsurifune, H. Mannen

    In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese and Australian beef. Two Bos indicus-specific markers and MCIR marker were used as possible candidate markers. Amplified fragment length polymorphism method was employed to develop additional candidate markers. The 1564 primer combinations provided three markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. In these markers, the allele frequencies in cattle from both countries were investigated for discrimination ability using PCR-RFLP. The probability of identifying Australian beef was 0.933 and probability of misjudgment was 0.017 using six selected markers. These markers could be useful for discriminating between Japanese and Australian beef and would contribute to the prevention of falsified breed labeling of meat. (c) 2007 Elsevier Ltd. All rights reserved.

    ELSEVIER SCI LTD, Oct. 2007, MEAT SCIENCE, 77 (2), 161 - 166, English

    [Refereed]

    Scientific journal

  • Genetic diversity of Bhutanese Cattle analyzed by mitochondrial DNA variation

    Lin B, Odahara S, Sasazaki S, Yamamoto Y, Namikawa T, Tanaka K, Dorji T, Tshering G, MUKAI Fumio, MANNEN Hideyuki

    2007, Journal of Animal Genetics, 35(1):5-10, 5 - 10, English

    [Refereed]

    Scientific journal

  • H. Ohsaki, M. Okada, S. Sasazaki, T. Hinenoya, T. Sawa, S. Iwanaga, H. Tsuruta, F. Mukai, H. Mannen

    Differences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was to identify candidate proteins causing differences of the meat qualities between the two breeds. Using technique of two-dimensional gel electrophoresis, protein profiling was compared from samples of the longissimus dorsi muscle and subcutaneous adipose tissue. Five protein spots were observed with different expression levels between breeds. By using LC-MS/MS analysis and Mascot program, three of them were identified as ankyrin repeat protein 2, phosphoylated myosin light chain 2 and mimecan protein. Subsequently, we compared the DNA coding sequences of three proteins between breeds to find any nucleotide substitution. However, there was no notable mutation which could affect pI or molecular mass of the proteins. The identified proteins may be responsible for different characteristics of the meat qualities between Japanese Black and Holstein cattle.

    Asian-Australasian Association of Animal Production Societies, 2007, Asian-Australasian Journal of Animal Sciences, 20 (5), 638 - 644, English

    [Refereed]

    Scientific journal

  • Mitochondrial DNA variation and genetic relationships in Japanese and Korean cattle

    S. Sasazaki, S. Odahara, C. Hiura, F. Mukai, H. Mannen

    The complete mtDNA D-loop regions of Japanese and Korean cattle were analyzed for their mtDNA variations and genetic relationships. Sequencing the 30 Higo substrain and 30 Tosa substrain of Japanese Brown, respectively 12 and 17 distinct Bos haplotypes were identified from 77 polymorphic nucleotide sites. In order to focus on the relationships among Japanese and Korean cattle, two types of phylogenetic tree were constructed using individual sequences; first, a neighbor-joining tree with all sequences and second, reduced median networks within each Japanese and Korean cattle group. The trees revealed that two major mtDNA haplotype groups, T3 and T4, were represented in Japanese and Korean cattle. The T4 haplogroup predominated in Japanese Black and Japanese Brown cattle (frequency of 43.3-66.7%), while the T3 haplogroup was predominant (83.3%) and T4 was represented only twice in the Korean cattle. The results suggested that the mitochondrial origins of Japanese Brown were Japanese ancient cattle as well as Japanese Black in despite of the considerable introgression of Korean and European cattle into Japanese Brown.

    ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Oct. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19 (10), 1394 - 1398, English

    [Refereed]

    Scientific journal

  • Breed discrimination using DNA markers derived from AFLP in Japanese beef cattle

    S Sasazaki, T Imada, H Mutoh, K Yoshizawa, H Mannen

    In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black x Holstein) breeds. The amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 1,754 primer combinations yielded eleven markers that were converted into single nucleotide polymorphism markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.9168 and probability of misjudgment was 0.0066 using four selected markers. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the prevention of falsified breed labeling of meat.

    ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Aug. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19 (8), 1106 - 1110, English

    [Refereed]

    Scientific journal

  • S. Sasazaki, T. Hinenoya, B. Lin, A. Fujiwara, H. Mannen

    In order to develop a comparative map between chicken and quail, we identified orthologous gene markers based on chicken genomic sequences and localized them on the Japanese quail Kobe-NIBS linkage map, which had previously been constructed with amplified fragment length polymorphisms. After sequencing the intronic regions of 168 genes located on chicken chromosomes 1-8, polymorphisms among Kobe-NIBS quail family parents were detected in 51 genes. These orthologous markers were mapped on eight Japanese quail linkage groups (JQG), and they allowed the comparison of JQG to chicken macrochromosomes. The locations of the genes and their orders were quite similar between the two species except within a previously reported inversion on quail chromosome 2. Therefore, we propose that the respective quail linkage groups are macrochromosomes and designated as quail chromosomes CJA 1-8.

    BLACKWELL PUBLISHING, Aug. 2006, ANIMAL GENETICS, 37 (4), 316 - 320, English

    [Refereed]

    Scientific journal

  • Mitochondrial DNA diversity of Korean native goats

    S Odahara, HJ Chung, SH Choi, SL Yu, S Sasazaki, H Mannen, CS Park, JH Lee

    Korean native goats have lived on the Korean peninsula for more than 2,000 Nears and are regarded as a valuable genetic resource for the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis and calculation of genetic diversities have been performed using mitochondrial DNA (mtDNA) sequence variations. A total of 19 Korean native goats were grouped into six haplotypes and the large majority of haplotypes were present in 13 animals, All mtDNA of these Korean goat; belonged to the mitochondrial (mt) lineage A and revealed remarkably small genetic distances within the Population when compared with other Asian goat populations, indicating less genetic variation in the Korean native goats. These results indicate high-inbred status of the Korean native goats and will influence breeding and conservation strategies adopted for this breed.

    ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Apr. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19 (4), 482 - 485, English

    [Refereed]

    Scientific journal

  • S Sasazaki, T Hinenoya, D Fujima, S Kikuchi, A Fujiwara, H Mannen

    In our previous study, a Kobe-NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA-AFLP fragments and localized these markers on the linkage map, Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament-deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.

    BLACKWELL PUBLISHING, Feb. 2006, ANIMAL SCIENCE JOURNAL, 77 (1), 42 - 46, English

    [Refereed]

    Scientific journal

  • SASAZAKI Shinji, MANNEN Hideyuki

    Japanese Society of Animal Breeding and Genetics, 2006, The journal of animal genetics, 34 (1), 41 - 45

  • S. Kikuchi, D. Fujima, S. Sasazaki, S. Tsuji, M. Mizutani, A. Fujiwara, H. Mannen

    The Japanese quail (Coturnix japonica) is a notably valuable egg and meat producer but has also been used as a laboratory animal. In the present study, we constructed a Japanese quail linkage map with 1735 polymorphic amplified fragment length polymorphisms markers, and nine chicken microsatellite (MS) markers, as well as sex and phenotypes of two genetic diseases: a muscular disorder (LWC) and neurofilament-deficient mutant (Quv). Linkage analysis revealed 578 independent loci. The resulting linkage map contained 44 multipoint linkage groups covering 2597.8 cM and an additional 218.2 cM was contained in 21 two-point linkage groups. The total map was 2816 cM in length with an average marker interval of 5.5 cM. The Quv locus was located on linkage group 5, but linkage was not found between the LWC locus and any of the markers. Comparative mapping with chicken using orthologous markers revealed chromosomal assignments of the quail linkage group 1 to chicken chromosome 2 (GGA2), 5 to GGA22, 2 to GGA5, 8 to GGA7, 27 to GGA11, 29 to GGA1 and 45 to GGA4. © 2005 International Society for Animal Genetics.

    Jun. 2005, Animal Genetics, 36 (3), 227 - 231, English

    Scientific journal

  • Shinji Sasazaki, Munehiro Usui, Hideyuki Mannen, Chihiro Hiura, Soichi Tsuji

    In order to estimate the influence of the Extension (E) locus in cattle coat color, the melanocortin-1 receptor (MC1R) gene in Japanese Black, Japanese Brown and Korean (Hanwoo) cattle were sequenced. The sequences of the coding region revealed three alleles (ED, E+ and e), which were previously reported. Polymerase chain reaction-restriction fragment length polymorphism was performed to investigate the gene frequencies of the three breeds. Japanese Black was almost composed of ED and E+ individuals, ED = 0.481 and E+ = 0.514, and no homozygous e/e, therefore that is consistent with the hypothesis that ED and E+ induce black pigment synthesis. Allele frequencies between Japanese Brown and Hanwoo were obviously different however, recessive red e allele frequency was 0.038 for Japanese Brown and 0.948 for Hanwoo, even though both breeds have quite similar coat colors (ranging from yellowish brown to dark brown including a red coat color). This result suggested that other genes are also associated with a coat color of red and brown in cattle.

    Apr. 2005, Animal Science Journal, 76 (2), 129 - 132, English

    Scientific journal

  • Development and Mapping of Microsatellite Markers derived from cDNA in Japanese Quail (Coturnix japonica)

    MANNEN Hideyuki, MURATA K, KIKUCHI S, FUJIMA D, SASAZAKI S, FUJIWARA A, TSUJI S

    Japan Poultry Science Association, 2005, J. Poult. Sci., 42:263-271 (3), 263 - 271, English

    Scientific journal

  • S. Sasazaki, K. Itoh, S. Arimitsu, T. Imada, A. Takasuga, H. Nagaishi, S. Takano, H. Mannen, S. Tsuji

    In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black × Holstein) breeds. Amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 500 primer combinations yielded six selected markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.882 and probability of misjudgment was 0.0198. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the elimination of falsified breed labeling of meat. © 2003 Elsevier Ltd. All rights reserved.

    Jun. 2004, Meat Science, 67 (2), 275 - 280, English

    Scientific journal

  • S. Tsuji, K. Itoh, S. Sasazaki, H. Mannen, K. Oyama, M. Shojo, F. Mukai

    Using amplified fragment length polymorphism (AFLP) fingerprinting, selective genotyping was performed to determine if this method was effective for selecting superior breeding stock. Forty-eight cows with extreme genetic merit for beef marbling score (BMS) were selected from a population of Japanese Black cattle (n = 4462), including 25 with the highest for predicted breeding value (PBV) and 23 with the lowest. Sixteen AFLP fragments were selected for further analysis based on fragment frequency differences between the high and low groups. A linear discriminant analysis using these AFLP fragments was applied in order to derive a discriminant function that classified the cows into high and low groups. Seven of the 16 fragments were included in the resulting function and the discriminant scores (general genetic values, GGV) of the 48 cows were calculated using the function. These cows were clearly separated into high and low groups by GGV with a correlation ratio of 0.91 (discriminative error of 2.1%). The same function was then applied to 121 additional cows that were randomly selected from the original population. A significant regression coefficient of GGV on BMS-PBV (R2 = 0.45) was obtained, which indicates that the GGV can be used as a selection criterion for BMS in this population. These results suggest that AFLP fingerprinting can be used for animal breeding without identifying the underlying genes affecting the trait of interest.

    Feb. 2004, Animal Genetics, 35 (1), 40 - 43, English

    Scientific journal

  • Selective genotyping for marker assisted selection in Japanese Black cattle.

    SASAZAKI S, ITOH K, URATSUJI H, MANNEN Hideyuki, OYAMA Kenji, SHOJO Masayuki, MUKAI Fumio, TSUJI S

    2004, Proc. 29th Int. Conf. on Anim. Genet., p.138, English

    International conference proceedings

  • S. Sasazaki, T. Honda, M. Fukushima, K. Oyama, H. Mannen, F. Mukai, S. Tsuji

    Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p< 0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.

    Asian-Australasian Association of Animal Production Societies, 2004, Asian-Australasian Journal of Animal Sciences, 17 (10), 1355 - 1359, English

    Scientific journal

  • Phylogenic analysis of five beef cattle breeds using AFLP marker.

    Sasazaki, S, Lee, E.J, Mannen, H, Kunieda, T, Sakurai, T, Yamauchi, K, Yeo, J.S, Tsuji, S

    2001, Anim. Sci. J., 72, 172

    [Refereed]

MISC

  • 兵庫県黒毛和種集団における枝肉の画像解析形質を対象としたゲノムワイド関連解析

    笹崎 晋史

    May 2019, 畜産技術, 768, 16 - 20

    [Invited]

  • 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

    山本雷斗, 川口芙岐, 秋山敬考, 吉田恵実, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    2019, 日本畜産学会大会講演要旨, 125th

  • mtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析

    村田佳子, 山本義雄, 西堀正英, MEIRAT Bakthin, POLAT Kazymbet, MELDELOKOV Alyan, MARATBEK Suleimenov, 笹崎晋史, 万年英之

    2018, 日本畜産学会大会講演要旨, 124th

  • 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

    中島彩華, 栖原功太郎, 小林栄治, 福島護之, 吉田恵実, 秋山敬孝, 小浜菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

    2018, 日本畜産学会大会講演要旨, 124th

  • ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

    川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    2018, 日本畜産学会大会講演要旨, 124th

  • mtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較

    田端里彩, 高橋幸水, 野村こう, 山縣高宏, 山本義雄, 山本義雄, 角田健司, 角田健司, 国枝哲夫, 伊藤文香, 竹内佳子, BAKHTIN Meirat, KAZYMBET Polat, MELDELOKOV Alyan, SULEIMENOV Maratbek, 西堀正英, 笹崎晋史, 万年英之

    2017, 日本畜産学会大会講演要旨, 122nd

  • Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.

    H. Ozaki, T. Tamura, K. Fukazawa, Y. Uemoto, M. Nishio, E. Kobayashi, T. Matsuhashi, S. Maruyama, T. Honda, K. Oyama, S. Sasazaki, H. Mannen

    AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, English

    Summary international conference

  • F. Kawaguchi, A. Nakajima, Y. Matsumoto, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, T. Honda, K. Oyama, H. Mannen, S. Sasazaki

    AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, English

    Summary international conference

  • A. Noda, S. Sasazaki, H. Mannen

    AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 18 - 18, English

    Summary international conference

  • A. Inoue, T. Nakajima, A. Nakajima, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, K. Oyama, T. Honda, H. Mannen, S. Sasazaki

    AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, English

    Summary international conference

  • ニワトリ筋ジストロフィーに関する研究

    松本大和, 笹崎晋史, 万年英之

    岡山実験動物研究会, Apr. 2014, 岡山実験動物研究会報, (30), 68 - 69, Japanese

  • ウシFABP4遺伝子のプロモーター領域に対する多型探索

    松本大和, 大山憲二, 万年英之, 笹崎晋史

    日本動物遺伝育種学会, 01 Dec. 2012, 動物遺伝育種研究, 40 (2), 78 - 78, Japanese

  • 岐阜県黒毛和種集団におけるUTS2R遺伝子内多型の脂肪酸組成に対する効果

    石井淳, 山地啓太, 阪本嵩幸, 秋山賢斗, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 松本大和, 笹崎晋史, 大山憲二, 万年英之

    01 Dec. 2012, 動物遺伝育種研究, 40 (2), 73, Japanese

  • 黒毛和種におけるLEP遺伝子内多型の脂肪酸組成に対する効果

    大倉一輝, 松本大和, 笹崎晋史, 大山憲二, 万年英之

    01 Dec. 2012, 動物遺伝育種研究, 40 (2), 85, Japanese

  • 黒毛和種におけるFASN遺伝子内多型の脂肪酸組成に対する効果

    阪本嵩幸, 石井淳, 山地啓太, 早川きり, 上本吉伸, 笹子奈々恵, 高須賀晶子, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 平野貴, 杉本喜憲, 松本大和, 笹崎晋史, 大山憲二, 万年英之

    01 Dec. 2012, 動物遺伝育種研究, 40 (2), 72, Japanese

  • 黒毛和種の枝肉形質に対するSCD,SREBP1,FASNおよびNCAPG遺伝子多型の関連解析

    西牧孝洋, 揖斐隆之, 斯琴図雅, 小林直彦, 松橋珠子, 今井佳積, 松井茉由, 上村圭一, 衛藤央好, 渡邉直人, 藤田達男, 齊藤陽介, 小松智彦, 万年英之, 笹崎晋史, 国枝哲夫

    01 Dec. 2012, 動物遺伝育種研究, 40 (2), 71, Japanese

  • プーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価

    加藤 大樹, 松本 大和, 笹崎 晋史, 秋山 敬孝, 吉田 恵実, 福島 護之, 野村 哲郎, 万年 英之

    (公社)日本畜産学会, Mar. 2012, 日本畜産学会大会講演要旨集, 115回, 118 - 118, Japanese

  • 兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造

    大倉一輝, 松本大和, 秋山敬孝, 吉田恵実, 福島護之, 笹崎晋史, 万年英之

    01 Dec. 2011, 動物遺伝育種研究, 39 (2), 142, Japanese

  • ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索

    山地啓太, 石井淳, 細川大介, 松本大和, 笹崎晋史, 大山憲二, 万年英之

    01 Dec. 2011, 動物遺伝育種研究, 39 (2), 133, Japanese

  • 黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析

    石井淳, 山地啓太, 阪本嵩幸, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 松本大和, 笹崎晋史, 万年英之

    01 Dec. 2011, 動物遺伝育種研究, 39 (2), 135, Japanese

  • 黒毛和種における各地域集団の遺伝的多様性と類縁関係の解析

    西牧孝洋, 揖斐隆之, 田邉義弘, 宮崎義之, 小林直彦, 松橋珠子, 秋山敬孝, 吉田恵実, 今井佳積, 松井茉由, 上村圭一, 渡邉直人, 藤田達男, 齊藤陽介, 万年英之, 笹崎晋史, 国枝哲夫

    01 Dec. 2011, 動物遺伝育種研究, 39 (2), 142, Japanese

  • Identification of the gene responsible for chicken muscular dystrophy

    Matsumoto H, SASAZAKI SHINJI, MANNEN HIDEYUKI

    The Korean Society of Poultry Science, Jun. 2011, Korean Journal of Poultry Science, 38 (2), 145 - 154, English

    [Refereed][Invited]

    Introduction scientific journal

  • ニワトリ筋ジストロフィー責任遺伝子の同定とモデル動物としての展開

    MANNEN HIDEYUKI, SASAZAKI SHINJI, 松本大和

    関西実験動物研究会, Dec. 2010, 関西実験動物研究会会報, 32, 1 - 12, Japanese

    [Invited]

    Introduction scientific journal

  • 松本大和, SASAZAKI SHINJI, MANNEN HIDEYUKI

    日本動物遺伝育種学会, Jun. 2010, 動物遺伝育種研究, 38 (1), 21 - 28, Japanese

    [Refereed]

    Introduction scientific journal

  • ニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析

    松本 大和, 笹崎 晋史, 藤原 哲, 菊池 建機, 市原 伸恒, 万年 英之

    (公社)日本畜産学会, Sep. 2009, 日本畜産学会大会講演要旨集, 111回, 41 - 41, Japanese

Books etc

  • アグリバイオー和牛の育種改良

    笹崎 晋史

    北隆館, Jan. 2020

  • 兵庫の和牛 但馬牛

    SASAZAKI SHINJI

    Others, 兵庫県農政環境部農林水産局畜産課, Mar. 2018, Japanese

    Scholarly book

  • 食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―「DNA鑑定を利用した牛肉偽装表示の防止」

    MANNEN HIDEYUKI, SASAZAKI SHINJI

    Others, ㈱シーエムシー出版, May 2011, Japanese

    Scholarly book

  • 食品表示を裏づける分析技術 科学の目で偽装を見破る 「品種鑑別 牛」

    MANNEN HIDEYUKI, SASAZAKI SHINJI

    Joint editor, 日本分析化学 表示・期限分析技術研究懇談会編, Nov. 2010, Japanese

    Scholarly book

Presentations

  • ミトゲノム解析によるアジア在来ヤギハプログループBの起源および伝播経路の推定

    井上真梨, 笹崎晋史, 川口芙岐, 田中啓介, 米澤隆弘, 呉佳齊, 万年英之

    日本動物遺伝育種学会第24回大会, 18 Nov. 2023

  • 兵庫県黒毛和種集団を用いた17番染色体における脂肪交雑QTLの探索

    伊藤ひかり, 足立亮仁, 川口芙岐, 万年英之, 笹崎晋史

    日本動物遺伝育種学会第24回大会, 18 Nov. 2023

  • 全ゲノムリシーケンスデータを用いたヤギ高地適応遺伝子多型の探索

    村井菜摘, 増子諒, 川口芙岐, 万年英之, 笹崎晋史

    日本動物遺伝育種学会第24回大会, 18 Nov. 2023

  • アジア在来ヤギにおける毛色の多様性に関わるMC1R遺伝子多型の探索

    釋 天音, 川口 芙岐, 万年 英之, 笹崎 晋史

    日本畜産学会第131回大会, 19 Sep. 2023

  • 高密度SNPマーカーを用いた東南アジア諸島在来ヤギの遺伝構造と伝播、遺伝子流入の推定

    増子諒, 高尾祐樹, アインアイン, 笹崎晋史, 川口芙岐, 山本義雄, Masangakay JS, Dagong MIA, Bugiwati SRA, Jiaqi Wu, 米澤隆弘, 万年英之

    日本畜産学会第131回大会, 19 Sep. 2023

  • 宮崎県黒毛和種集団を用いたウシ7番染色体における脂肪交雑に対する原因多型の探索

    森石 宥璃華, 川口 芙岐, 万年 英之, 笹崎 晋史

    日本畜産学会第131回大会, 19 Sep. 2023

  • Genome-Wide Association Study for the Percentage of Oleic Acid in Japanese Black Cattle Revealed the Candidate Region on BTA19

    Fuki Kawaguchi, Natsuki Maeda, Kenji Oyama, Emi Yoshida, Eiji Iwamoto, Namiko Kohama, Shinji Sasazaki, Hideyuki Mannen

    30th Plant & Animal Genome Conference, 16 Jan. 2023, English

  • Verification of Candidate SNP Effects Reveals Two QTLs on BTA7 for Beef Marbling in Two Japanese Black Cattle Populations

    Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Namiko Kohama, Emi Yoshida, Eiji Iwamoto, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

    30th Plant & Animal Genome Conference, 16 Jan. 2023, English

  • 岩手県黒毛和種における白斑とMITF遺伝子多型との関連

    金丸智香, 西和隆, 川口芙岐, 万年英之, 笹崎晋史

    日本動物遺伝育種学会第23回大会, 16 Nov. 2022

  • 兵庫県黒毛和種におけるゲノムワイド関連解析を用いたオレイン酸含有率QTLの探索

    前田菜月, 小浜菜美子, 秋山敬孝, 吉田恵実, 岩本英治, 大山憲二, 笹崎晋史, 万年英之, 川口芙岐

    日本畜産学会第130回大会, 16 Sep. 2022

  • アジア在来ヤギにおける角の有無と既報の1番染色体無角関連多型との関連

    森澤亮, 笹崎晋史, 万年英之, 川口芙岐

    日本動物遺伝育種学会第22回大会, 13 Nov. 2021

  • 父母系および核ゲノムSNPマーカーを用いたインドネシア在来ヤギの遺伝構造解析及び遺伝子流入の推定

    政岡真帆, 笹崎晋史, 川口芙岐, 米澤隆弘, 呉 佳齊, 山本義雄, DAGONG MIA, BUGIWATI SRA, 万年英之

    日本動物遺伝育種学会第22回大会, 13 Nov. 2021

  • 黒毛和種現場後代検定調査牛集団における全ゲノムリシーケンスデータを用いたオレイン酸含有率に関わる多型の探索

    緋田泰良, 笹崎晋史, 大山憲二, 万年英之, 川口芙岐

    日本畜産学会第129回大会, 15 Sep. 2021

  • Confirmation of quantitative trait locus location on BTA19 for the percentage of oleic acid in beef based on effects of 5 polymorphisms and linkage disequilibrium analysis in 2 Japanese Black cattle populations.

    Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

    38th International Society for Animal Genetics Conference, Jul. 2021

  • 父母系および高密度 SNPマーカーを用いたフィリ ピン在来ヤギの遺伝構造と伝播の推定

    AYIN AYIN, 田端 里彩, 笹崎 晋史, 川口 芙岐, 小林 栄治, 山本 義雄, 米澤 隆弘, 呉 佳齊, Masangkay J, 万年 英之

    日本畜産学会第128回大会, 28 Mar. 2021

  • 50K SNPアレイを用いた和牛4品種に関する多様性および類縁関係の調査

    中村充暉, 鈴木啓一, 小林栄治, 万年英之, 笹崎晋史, 川口芙岐

    日本畜産学会第128回大会, 28 Mar. 2021

  • ネパール在来ヤギにおけるEPAS1, FGF5遺伝子多型と高地適応形質との関連調査

    冨田宏一郎, 野村悠登, 笹崎晋史, 万年英之

    第21回日本動物遺伝育種学会大会, 21 Nov. 2020

  • 集積流体回路を用いた兵庫県黒毛和種集団におけるロース脂肪割合候補多型の効果の検証

    近藤陽菜, 小浜菜美子, 秋山敬孝, 吉田恵実, 小林栄治, 川口芙岐, 万年英之, 笹崎晋史

    日本動物遺伝育種学会第21回大会, 21 Nov. 2020

  • ミトゲノム配列による ハプログループT4の系統解析

    角谷 菜帆, 村田 佳子, 川口 芙岐, 笹崎 晋史, 万年 英之

    第20回日本動物遺伝育種学会大会, 30 Nov. 2019

  • Validation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic Circuit

    Yamamoto R, Toyomoto S, Kohama N, Akiyama T, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

    37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

    Poster presentation

  • Phylogenetic analysis of Nepalese goats using mtDNA D-loop region and SRY gene sequences

    Nomura Y, Kunieda T, Shah M, Kawaguchi F, Sasazaki S, Mannen H

    37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

    Poster presentation

  • Genetic diversity of Bos taurus and Bos indicus using developed indel markers

    Yasui T, Yamanaka H, Kawaguchi F, Sasazaki S, Yonezawa T, Wu J, Mannen H

    37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

    Poster presentation

  • Genetic diversity and structure in 11 native Asian goat populations analyzed by high density SNP array

    Iso K, Sasazaki S, Kawaguchi F, Yonezawa T, Wu J, Nomura K, Takahashi Y, Kobayashi E, Shah M, Faruque O, Masangkay JS, Bakhtin M, Kazymbet P, Dorji T, Mannen H

    37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

    Poster presentation

  • Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations

    Kawaguchi F, Kigoshi H, Yasuzumi R, Oyama K, Mannen H, Sasazaki S

    37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

    Poster presentation

  • Molecular epidemiological study of bovine leukemia virus in Zebu cattle (Bos indicus)

    Nishikaku K, Yonezawa T, Murakami S, Torii Y, Kawaguchi F, Sasazaki S, Mannen H, Kobayashi T

    The 2nd Joint Meeting of Veterinary Science in East Asia, Apr. 2019, English, Seoul, Korea, International conference

    Oral presentation

  • 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

    山本雷斗, 川口芙岐, 秋山 敬孝, 吉田 恵実, 小浜 菜美子, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

    第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conference

    Oral presentation

  • mtDNA D-loop領域とSRY遺伝子の塩基配列に基づいたネパール在来ヤギの遺伝的類縁関係

    野村悠登, 国枝哲夫, Manoj Shah, 笹崎晋史, 川口芙岐, 万年英之

    第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conference

    Oral presentation

  • 兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出

    木越洸利, 川口芙岐, 大山憲二, 万年英之, 笹崎晋史

    第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

    Oral presentation

  • ゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索

    安栖梨理, 井上麻美, 小林 栄治, 福島 護之, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

    第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

    Oral presentation

  • カザフスタン在来ヤギに対するmtDNAとY染色体の塩基配列解析~Eurasian Steppeは重要なヤギの伝播経路の一つである~

    田端里彩, 笹崎晋史, 山本義雄, Meirat BAKHTIN, Polat KAZYMBET, Meldelokov ALYAN, Maratbek Z SULEIMENOV, 西堀正英, 万年英之

    第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

    Oral presentation

  • Effect of SLC27A6 gene K81M polymorphism on fat percentage in rib-eye area in Japanese Black cattle

    Toyomoto S, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

    PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conference

    Poster presentation

  • Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data

    Kawaguchi F, Kigoshi H, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

    PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conference

    Poster presentation

  • 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

    中島彩華, 栖原功太郎, 小林 栄治, 福島 護之, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

    第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

    Oral presentation

  • ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

    川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

    Oral presentation

  • mtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析

    村田佳子, 山本義雄, 西堀正英, Bakhtin M, Kazymbet P, Alyan M, Suleimenov MZ, 笹崎晋史, 万年英之

    第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

    Oral presentation

  • 兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果

    木越洸利, 川口芙岐, 上本吉伸, 福島護之, 吉田恵実, 岩本英治, 秋山敬孝, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference

    Oral presentation

  • 複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討

    川口芙岐, 木越洸利, 安栖梨理, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference

    Oral presentation

  • 北海道ホルスタインは希少mtDNAハプログループPを有する

    野田葵, 笹崎晋史, 小林栄治, 万年英之

    第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

    Oral presentation

  • ゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索

    中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

    第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

    Oral presentation

  • Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析

    山中颯, 笹崎晋史, Hla Hla Moe, Moe Lwin, 下桐猛, 万年英之

    第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

    Oral presentation

  • PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle

    Kawaguchi F, kigoshi H, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

    36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

    Poster presentation

  • Phylogenetic analysis of Kazakhstani goats using mtDNA HV1 and SRY gene sequences

    Tabata R, Sasazaki S, Bakhtin M, Kazymbet P, Alyan M, Suleimenov M, Nishibori M, Mannen H

    36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

    Poster presentation

  • Genome-wide association study revealed a candidate region for beef marbling on BTA7 in Japanese Black cattle

    Sasazaki S, Nakajima T, Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H

    36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

    Poster presentation

  • Genetic structure and relationships among 11 cattle populations using Indel markers

    Yamanaka H, Sasazaki S, Moe HH, Lwin M, Shimogiri T, Mannen H

    36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

    Poster presentation

  • Effects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows

    Tsuchimura M, Fukazawa K, Matsuhashi T, Maruyama S, Oyama K, Sasazaki S, Mannen H

    36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

    Poster presentation

  • Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle

    Kigoshi H, Matsumoto Y, Kawaguchi F, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Oyama K, Honda T, Mannen H, Sasazaki S

    36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

    Poster presentation

  • mtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較

    田端里彩, 高橋幸水, 野村こう, 山縣高宏, 山本義雄, 角田健司, 国枝哲夫, 伊藤文香, 竹内佳子, Bakhtin M, Kazymbet P, 西堀正英, Alyan M, Suleimenov MZ, 笹崎晋史, 万年英之

    第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conference

    Oral presentation

  • 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連

    川口芙岐, 深澤謙治, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    第17回日本動物遺伝育種学会大会, Nov. 2016, Japanese, 日本動物遺伝育種学会, 名古屋, Domestic conference

    Oral presentation

  • Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle

    Ozaki H, Tamura T, Fukazawa K, Uemoto Y, Nishio M, Kobayashi E, Matsuhashi T, Maruyama S, Honda T, Oyama K, Sasazaki S, Mannen H

    35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

    Poster presentation

  • Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black

    Kawaguchi F, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

    35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

    Poster presentation

  • Genome-wide association study identifies a QTL for fat percentage in rib-eye area on BTA10 in Japanese Black cattle

    Inoue A, Nakajima T, Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H, Sasazaki S

    35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

    Poster presentation

  • Genetic diversity and origin of mtDNA haplogroup P observed in Japanese Shorthorn

    Noda A, Sasazaki S, Mannen H

    35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

    Poster presentation

  • 日本短角種において観察される希少なmtDNAハプログループPの母系起源

    野田葵, 万年英之, 笹崎晋史

    第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference

    Oral presentation

  • ウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索

    井上麻美, 中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年英之, 笹崎晋史

    第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference

    Poster presentation

  • 兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析

    松本祐汰, 中島彩華, 上本 吉伸, 小林栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 笹崎晋史, 本多健, 大山憲二, 万年英之

    第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

    Poster presentation

  • 黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定

    深澤謙治, 笹崎晋史, 大山憲二, 本多健, 小林栄治, 福島 護之, 秋山 敬孝, 小浜 菜美子, 万年英之

    第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

    Poster presentation

  • 岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析

    田村朋子, 深澤謙治, 尾崎弘樹, 上本吉伸, 西尾元秀, 小林栄治, 松橋珠子, 丸山新, 本多健, 笹崎晋史, 大山憲二, 万年英之

    第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

    Poster presentation

  • GWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索

    中嶋翼, 中島彩華, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

    第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

    Poster presentation

  • 黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果

    川口芙岐, 大倉一輝, 大山憲二, 万年英之, 笹崎晋史

    第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference

    Oral presentation

  • 高密度SNPアレイによる口之島牛の遺伝的多様性解析

    齊藤 祐也, 下桐 猛, 笹崎晋史, 万年英之

    第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference

    Oral presentation

  • ホルスタイン種におけるFASN-g.841 及びg.16024 多型の乳形質に対する効果

    早川きり, 阿部剛, 長谷部浩行, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

    第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

    Poster presentation

  • SRY 遺伝子とmtDNA 塩基配列を用いたシバヤギの遺伝的多様性解析

    脇愛生, 笹崎晋史, 小林栄治, 万年英之

    第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

    Poster presentation

  • DigiTag2 assay を用いたアジア牛集団に対する遺伝的類縁関係と遺伝的構造の解析

    米坂陸, 笹崎晋史, 安江博, 庭田悟, 向井文雄, 万年英之

    第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

    Poster presentation

  • 50K SNP チップを用いた口之島野生化牛の遺伝的多様性に関する研究

    下桐猛, 吉田彩華, 笹崎晋史, 片平清美, 大島一郎, 河邊弘太郎, 岡本新, 印牧美佐生, 国枝哲夫, 万年英之

    第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

    Poster presentation

  • The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle

    Hayakawa K, Ishii A, Yamaji K, Uemoto Y, Sasago N, Kobayashi E, Kobayashi N, Matsuhashi T, Maruyama S, Matsumoto H, Sasazaki S, Mannen H

    34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

    Oral presentation

  • Paternal genetic structure of Asian goats using SRY gene

    Waki A, Sasazaki S, Kobayashi E, Mannen H

    34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

    Oral presentation

  • LPL gene polymorphisms are associated with fatty acid composition in Japanese Black

    Koga S, Oyama K, Sasazaki S, Mannen H

    34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

    Oral presentation

  • Genome wide association study revealed candidate regions for calving interval in Japanese black cattle

    Kawasaki Y, Fukazawa K, Fujii T, Uemoto Y, Fukushima M, Akiyama T, Kohama N, Kobayashi E, Oyama K, Sasazaki S, Mannen H

    34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

    Oral presentation

  • Genetic diversity and relationship among 16 Asian and European cattle populations using 121 autosomal SNPs genotypes by the DigiTag2 assay

    Yonesaka R, Sasazaki S, Yasue H, Niwata S, Mukai F, Mannen H

    34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

    Oral presentation

  • A genome wide association study for beef marbling and fatty acid composition in Japanese Black cattle using pooled DNA

    Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Sasazaki S, Mannen H

    34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

    Oral presentation

  • 黒毛和種におけるLEP遺伝子プロモーター領域に対する多型探索

    大倉 一輝, 笹崎 晋史, 大山 憲二, 万年 英之

    第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conference

    Poster presentation

  • mtDNA塩基配列を用いた口之島野生化牛、日本短角種、無角和種の遺伝的多様性解析

    米坂 陸, 下桐猛, 稲吉 洋裕, 印牧 美佐生, 国枝 哲夫, 向井 文雄, 笹崎 晋史, 万年 英之

    第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conference

    Poster presentation

  • ウシ脂肪酸組成に対するFASN 遺伝子内多型の効果

    早川 きり, 阪本 嵩幸, 石井 淳, 山地 啓太, 上本 吉伸, 笹子 奈々恵, 高須賀 晶子, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 野偽 卓哉, 田淵 一郎, 平野 貴, 松本大和, 笹崎 晋史, 大山 憲二, 万年 英之

    第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conference

    Oral presentation

  • SRY遺伝子を用いたアジア在来家畜ヤギの多様性解析

    脇 愛生, 万年 英之, 笹崎 晋史

    第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conference

    Oral presentation

  • 黒毛和種の枝肉形質に対するSCD、SREBP1、FASN およびNCAPG 遺伝子多型の関連解析

    西牧 孝洋, 揖斐 隆之, 斯琴 図雅, 小林 直彦, 松橋 珠子, 今井 佳積, 松井 茉由, 上村 圭一, 衛藤 央好, 渡邉 直人, 藤田 達男, 齊藤 陽介, 小松 智彦, 万年 英之, 笹崎 晋史, 国枝 哲夫

    第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

    Poster presentation

  • 黒毛和種におけるLEP 遺伝子内多型の脂肪酸組成に対する効果

    大倉 一輝, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

    第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

    Poster presentation

  • 黒毛和種におけるFASN 遺伝子内多型の脂肪酸組成に対する効果

    阪本 嵩幸, 石井 淳, 山地 啓太, 早川 きり, 上本 吉伸, 笹子 奈々恵, 高須賀 晶子, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 平野 貴, 杉本 喜憲, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

    第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

    Poster presentation

  • 牛肉中脂肪の脂肪酸組成改良に向けた新規遺伝子マーカーの探索及び効果の検証

    SASAZAKI SHINJI

    第6回家畜DNA西郷シンポジウム, Oct. 2012, Japanese, 独立行政法人家畜改良センター, Domestic conference

    Public discourse

  • 岐阜県黒毛和種集団におけるUTS2R 遺伝子内多型の脂肪酸組成に対する効果

    石井 淳, 山地 啓太, 阪本 嵩幸, 秋山 賢斗, 上本 吉伸, 笹子 奈々恵, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

    第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

    Poster presentation

  • ウシFABP4 遺伝子のプロモーター領域に対する多型探索

    松本 大和, OYAMA KENJI, MANNEN HIDEYUKI, SASAZAKI SHINJI

    第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 仙台, Domestic conference

    Poster presentation

  • UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle

    Sasazaki Shinji, Akiyama Kento, Narukami Takahiro, Matsumoto Hirokazu, Oyama Kenji, Mannen Hideyuki

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • The SNPs in the ACACA gene effective on fatty acid composition in Holstein milk

    Sakamoto Takayuki, Matsumoto Hirokazu, Sasaki Kenta, Bessho Takuya, Kobayashi Eiji, Abe Tsuyoshi, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • The SNP in the promoter region of the bovine ELOVL5 gene is effective on subcutaneous fat thickness

    MATSUMOTO Hirokazu, SHIMIZU Yumiko, TANAKA Atsuko, NOGI Takuya, TABUCHI Ichiro, Oyama Kenji, Mannen Hideyuki, Sasazaki Shinji

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • Mutations in the PLAG1 region are associated with height, weight, puberty, IGF1, and fat deposition in cattle

    Fortes M. R. S, Sasazaki Shinji, Kemper K. E, Reverter A, Pryce J, Barendse W, Bunch R, Zhang Y. D, Hawken R. J, Goddard M. E, Lehnert S. A

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • Identification of quantitative trait loci affecting economic traits based on divergently selected regions between Japanese Black and Holstein cattle

    YAMAJI Keita, HOSOKAWA Daisuke, ISHII Atsushi, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • Genome wide association study for fatty acid composition in Japanese Black cattle

    Ishii Atsushi, Yamaji K, Uemoto Y, Kobayashi E, Sasago N, Kobayashi N, Matsuhashi T, Maruyama S, Matsumoto H, Sasazaki Shinji, Mannen Hideyuki

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • Genetic diversity and structure in goat analyzed by newly-developed SNPs

    MANNEN HIDEYUKI, Lin BZ, Kato T, Kaneda M, SASAZAKI SHINJI

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • Evaluation of genetic diversity in Japanese Black cattle population using SNP-MaP strategy

    Kato Taiki, Matsumoto H, Sasazaki Shinji, Akiyama T, Fukushima M, Yoshida E, Nomura T, Mannen Hideyuki

    33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

    Poster presentation

  • プーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価

    加藤 大樹, 松本 大和, 笹崎 晋史, 秋山 敬孝, 吉田 恵実, 福島 護之, MANNEN HIDEYUKI

    第115回日本畜産学会大会, Mar. 2012, Japanese, 名古屋, Domestic conference

    Oral presentation

  • 兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造

    大倉一輝, 松本大和, 秋山敬孝, 吉田恵実, 福島護之, 笹崎晋史, 万年英之

    第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

    Poster presentation

  • 黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析

    石井 淳, 山地啓太, 阪本嵩幸, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山 新, 松本大和, 笹崎晋史, 万年英之

    第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

    Poster presentation

  • 黒毛和種およびホルスタインにおけるUTS2R遺伝子多型の脂肪酸組成に対する効果

    秋山 賢斗, 鳴神 貴太, 笹崎 晋史, 大山 憲二, 万年 英之

    第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

    Poster presentation

  • ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索

    山地 啓太, 石井 淳, 細川 大介, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

    第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

    Poster presentation

  • 黒毛和種閉鎖集団における形質関連遺伝子頻度の経時的変化

    加藤 大樹, 松本 大和, 笹崎 晋史, 秋山 敬孝, 吉田 恵実, 福島 護之, 万年 英之

    第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference

    Oral presentation

  • ウシELOVL5とELOVL6遺伝子のプロモーター領域に対する多型探索

    清水 裕美子, 松本 大和, 笹崎 晋史, 万年 英之

    第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference

    Oral presentation

  • DigiTag2 アッセイを用いたウシ個体識別及び親子鑑定システムの確立

    梶山 亮介, 原 和弘, 笹崎 晋史, 松本 大和, 安江 博, 向井 文雄, 万年 英之

    第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference

    Oral presentation

  • 黒毛和種集団におけるsliding window approachを利用した枝肉形質関連遺伝子座の探索

    細川 大介, 石井 淳, 山地 啓太, 笹崎 晋史, 大山 憲二, 万年 英之

    第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conference

    Poster presentation

  • ウシELOVL遺伝子の塩基配列決定及び多型探索

    清水 裕美子, 笹崎 晋史, 万年 英之

    第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conference

    Poster presentation

  • Polymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattle

    Narukami Takahiro, Bessyo Takuya, Tanaka Atsuko, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

    32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

    Poster presentation

  • Identification of divergently selected regions between Japanese Black and Holstein cattle based on a bovine 50k SNP array

    Yamaji Keita, Hosokawa Daisuke, Sasazaki Shinji, Mannen Hideyuki

    32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

    Poster presentation

  • Genetic diversity and structure in Bos taurus and Bos indicus populations analyzed by SNP markers

    Lin BZ, Sasazaki Shinji, Mannen Hideyuki

    32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

    Poster presentation

  • Development of discrimination markers between Japanese domestic and imported beef based on a bovine 50K SNP array

    Sasazaki Shinji, Hosokawa Daisuke, Ishihara Rie, Aihara Hiroki, Oyama Kenji, Mannen Hideyuki

    32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

    Poster presentation

  • Allele frequencies of gene polymorphisms related to economic traits in Bos taurus and Bos indicus cattle breeds

    Kaneda Makoto, Shinji Sasazaki, Mannen Hideyuki

    32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

    Poster presentation

  • 黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果

    鳴神 貴太, 笹崎 晋史, 大山憲二, 庄司 則章, 中嶋 宏明, 万年 英之

    第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

    Oral presentation

  • バングラデシュにおける 在来家畜及び野生原種の遺伝資源学的研究

    高橋絢子, 万年英之, 林 邦忠, 笹崎晋史, 山本義雄, 黒澤弥悦, 野村こう, 高橋幸水, 菅野雅子, Khan M.Y.A, Husain Z.M.A, Sarder M.J.U, Aziz S.A, Husain S.S, Omar Faruque MD, 天野卓

    第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

    Oral presentation

  • SNPマーカーを用いたウシの遺伝的多様性と遺伝構造解析

    林 邦忠, 笹崎晋史, 万年英之

    第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

    Oral presentation

  • Sampling method for estimating neutral allele frequency in a pedigreed population

    HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, NOMURA Tetsuro, MUKAI Fumio

    9th World Congress of Genetics Applied to Livestock Production, 2010, English, International conference

    Poster presentation

  • ホルスタイン種におけるFASN遺伝子の多型探索及び乳形質の効果

    稲田 聡士, 笹崎 晋史, 大山憲二, 真崎匡, 長谷部浩行, 阿部剛, 万年 英之

    第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conference

    Poster presentation

  • Bovine 50k SNPs情報を利用したウシゲノム解析

    細川 大介, 笹崎 晋史, 万年 英之

    第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conference

    Poster presentation

  • 国産牛と米国産牛とのDNA鑑定マーカーの開発

    相原 浩揮, 細川 大介, 石原 理恵, 末川 裕, 荒木 舞, 笹崎 晋史, 万年 英之

    第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

    Oral presentation

  • ホルスタイン種における枝肉形質に関る遺伝子多型の効果

    鳴神 貴太, 笹崎 晋史, 大山 憲二, 野儀 卓哉, 万年 英之

    第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

    Oral presentation

  • ニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析

    松本 大和, 笹崎 晋史, 藤原 哲, 菊池 建機, 市原 伸恒, 万年 英之

    第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

    Oral presentation

  • ウシ疾病関連遺伝子、抗病性関連遺伝子の多様性解析

    金田 真, 笹崎 晋史, 国枝 哲夫, 万年 英之

    第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

    Oral presentation

  • 黒毛和種の個体識別および親子鑑定を目的としたSNPsパネルの開発

    紺 有加里, 原 和弘, 笹崎 晋史, 万年 英之

    第110回日本畜産学会大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conference

    Oral presentation

  • ウシ経済形質関連遺伝子の多様性解析

    金田 真, 笹崎 晋史, 万年 英之

    第110回日本畜産学会大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conference

    Oral presentation

Research Projects

  • International joint research to elucidate the propagation of domestic animals across the Indian Ocean

    万年 英之, 米澤 隆弘, 下桐 猛, 笹崎 晋史, 川口 芙岐, 西堀 正英

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Kobe University, 07 Oct. 2021 - 31 Mar. 2026

    【研究の目的】本国際共同研究は「海の道」による家畜の海上伝播とその影響を明らかにすることを目的とし、着想したインド洋海上経路が中小家畜の伝播経路の一つであるという新しい仮説を検証する。その具体的方策として、インド洋海上経路の複数国(インドネシア、モーリシャス、マダガスカルなど)と国際共同研究を構築し、収集する中小家畜(ヤギ、ブタ、ニワトリ)試料に対し全ゲノムリシーケンスを含むゲノムワイドな遺伝学的解析を実施する。解析情報から広大なインド洋海路が家畜の伝播や遺伝子流入に与えた影響を評価し、ヒトの歴史に紐づけた家畜伝播の考察を行う。この解析には、家畜情報と試料(DNA)の収集という観点から、インド洋広範囲にわたる国際共同研究が必須となる。本課題により、インド洋海路に基づく新しい国際共同研究コンソーシアムを構築し、若手研究者参画によるグローバル視点を持つ将来の国際共同研究のリーダーを育成する。 【2021年度の研究実績】当該年度は世界的なコロナ禍が継続中のため、オンラインを使い国際共同研究者と調査計画を立案した。調査対象国はインドネシアとモーリシャスである。モーリシャスはFAREIのGobindram博士と連絡を取り、コロナ禍の状況を注視しながら2022年9月頃に2-3週間の調査を行う計画を立て、対象動物はヤギとニワトリである。モーリシャス本島とロドリゲス島の2島に対して調査を計画しつつある。インドネシアはHasanuddin大学のBugiwati 教授と連絡を取り、2022年度の調査計画を進めている。Lakor島、Timor島、Lembata島を対象とし、対象家畜はヤギである。コロナ禍の状況を鑑み、インドネシア共同研究者による試料収集を計画を進めている。採取された試料は冷凍保存し、機会を見て代表者が現地でのDNA精製等を行う予定としている。

  • ウシ脂肪交雑形成機構の解明に向けた候補遺伝子変異の網羅的検証

    笹崎 晋史

    科学研究費補助金/基盤研究(C), Apr. 2022 - Mar. 2025, Principal investigator

  • Have East native cattle received genetic introgression from wild cattle? -evaluation of the genetic structure and propagation route-

    万年 英之, 川口 芙岐, 笹崎 晋史, 米澤 隆弘

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2021 - 31 Mar. 2024

    本課題では、ユーラシア大陸を網羅する北方系家畜ウシ集団に対し、遺伝様式が異なるmtDNA解析、高密度SNPアレイ解析、全ゲノムリシーケンスなどによるビックデータを用いた網羅的な遺伝構造解析を実施し、その遺伝子流入、遺伝構造、起源、伝播経路などを包括的に明らかにする。その成果を通して、東アジア家畜ウシの遺伝資源保護・開発につなげ、次世代畜産分野に対する学術的知見および基礎情報を得ることを目的とする。 家畜ウシのハプログループT4は東アジア地域で特異的に観察され、特に和牛品種ではこのハプログループT4が他の東アジア地域に比べ、高頻度で観察されている。そこで本課題では、和牛品種として日本短角種と黒毛和種におけるハプログループT4のミトゲノム配列決定をすることで、ハプログループT4の系統解析や分岐年代の推定を行い、ハプログループT4の分岐・成立過程を明らかにすることを目的とした。また高密度SNPアレイを用いた解析を、和牛4品種およびアジア品種、西洋品種の遺伝子型データを得て様々な遺伝的解析を実施した。 ミトゲノム解析の結果、和牛品種は韓牛と同じクラスターに含まれ、先行研究と同様にハプログループT4はサブハプログループT3aから分岐したものであることが明らかとなった。分岐年代推定より、ハプログループT4は家畜化中心地である西アジアの肥沃な三日月地帯からシルクロードの内、温暖な環境で水が豊富に存在するオアシスの道を経て、東のアジアへと伝播する過程において生じたハプログループであると考えられた。高密度SNPアレイを用いた解析では、和牛の多様性が他の海外品種と比較して全体的に低い傾向が示された。

  • 全ゲノム情報を用いたヤギの起源と伝播、環境適応形質の包括的解明に向けた先駆的研究

    万年 英之, 笹崎 晋史, 米澤 隆弘

    日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), 神戸大学, 28 Jun. 2019 - 31 Mar. 2023

    本研究では、世界規模で収集されたヤギのDNA試料に対し、全ゲノム解析を用いた大規模DNA情報解析を利用した起源・伝播経路の解明と環境適応形質遺伝子の全貌解明を着想し、その足掛かりとなる包括的研究を実施することを目的とした。 本年度は、mtDNAの東アジアにおける頻度分布調査とmtDNA全塩基配列決定による解析(ミトゲノム解析)、高密度SNP解析を中心に研究を進め、概ねの解析は終了した。その結果として、1) ハプログループBはパキスタン以東で高頻度に観察された。これは、ハプログループBが他のハプログループと異なる起源地を持つという先行研究を支持する結果となった。2) 12個体の東南アジア在来ヤギのミトゲノム配列の増幅に成功し、12のハプロタイプが観察された。さらにミトゲノムを使用した先行研究の配列データを用いてベイズ系統樹を作成した結果、B1が11個体、B2が1個体得られた。次に得られた系統樹を用いて、ベイズ法と最尤法で年代推定を行った結果、サブハプログループB1とB2の分岐年代は30,1600~307,700年前と推定された。3) 高密度SNP解析として、19 集団1,472 個体を本研究のデータとして使用し、14集団732個体 39,020 SNPを用いた解析を行った。系統解析からは地理的に近い集団がまとまって確認される地理的位置関係に即した結果が得られた。遺伝構造解析より、フィリピンでは東南アジア及び南アジア集団の中で唯一、スイスで優勢な遺伝的要素が確認された。また、フィリピン集団はスイスおよび南アフリカ共和国との遺伝的類似性が示唆される結果となった。一方、インドネシアでは父系統解析によりスイス・南アフリカ共和国方面からの遺伝子流入が推定されたが、核ゲノムSNP解析ではそれら地域との遺伝混合は認められなかった。

  • 笹崎 晋史

    科学研究費補助金/基盤研究(C), Apr. 2018 - Mar. 2021, Principal investigator

    Competitive research funding

  • 万年 英之

    科学研究費補助金/基盤研究(B), Apr. 2017 - Mar. 2020

    Competitive research funding

  • 万年 英之

    科学研究費補助金/基盤研究(B), Apr. 2016 - Mar. 2020

    Competitive research funding

  • 万年 英之

    学術研究助成基金助成金/挑戦的萌芽研究, Apr. 2016 - Mar. 2018

    Competitive research funding

  • 万年 英之

    学術研究助成基金助成金/挑戦的萌芽研究, Apr. 2014 - Mar. 2016

    Competitive research funding

  • 大山 憲二

    科学研究費補助金/基盤研究(C), Apr. 2012 - Mar. 2015

    Competitive research funding

  • 万年 英之

    科学研究費補助金/基盤研究(B), Apr. 2011 - Mar. 2014

    Competitive research funding

  • 笹崎 晋史

    科学研究費補助金/若手研究(B), Apr. 2008 - Mar. 2010, Principal investigator

    Competitive research funding

  • Studies on fatty acid metabolism related genes in cattle

    SASAZAKI Shinji

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Kobe University, 2008 - 2009

    Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of seven genes (FABP4、LXRα、Cyt b5、ACSL1、ACSL4、DGAT 2 and ACACA) and fatty acid composition.Sequence comparisons revealed 14 single nucleotide polymorphisms in seven genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXRα, were nonsynonymous substitutions. The genotypes of FABP4 I74V was significantly associated with palmitoleic acids (C16:1, P=0.0086) content in intramuscular fat. Especially, FABP4 I74V had highly significant effect (P<0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.

Industrial Property Rights

  • ウシ由来試料を鑑定する方法及びキット

    MANNEN HIDEYUKI, SASAZAKI SHINJI

    特願2010-095158, 16 Apr. 2010, 大学長, 特許5800307, 04 Sep. 2015

    Patent right

  • 非黒毛和種か否かを鑑定する方法及びキット

    MANNEN HIDEYUKI, SASAZAKI SHINJI

    特願2009-223172, 28 Sep. 2009, 大学長, 特許5729897, 17 Apr. 2015

    Patent right

  • 国産牛と豪州産輸入牛の鑑別方法

    MANNEN HIDEYUKI, SASAZAKI SHINJI, 向井 文雄

    特願2006-224984, 22 Aug. 2006, 大学長, 特許4872084, 02 Dec. 2011

    Patent right