Directory of Researchers

YAMASHITA Yoko
Graduate School of Agricultural Science / Department of Agrobioscience
Associate Professor
Agriculture / Other Field
Last Updated :2023/12/12

Researcher Profile and Settings

Affiliation

  • <Faculty / Graduate School / Others>

    Graduate School of Agricultural Science / Department of Agrobioscience
  • <Related Faculty / Graduate School / Others>

    Faculty of Agriculture / Department of Agrobioscience

Teaching

Research Activities

Research Areas

  • Life sciences / Food sciences

Awards

  • Mar. 2022 公益財団法人日本農芸化学会, JSBBA Award for Young Investigators, 難吸収性食品成分の組織間ネットワークを介した生理機能に関する研究

  • Jul. 2021 Japan Society of Nutrition and Food Science, JSNFS Award for Young Investigators, ポリフェノールの消化管シグナルを介した生体調節機能解明に関する研究

  • Nov. 2020 公益財団法人農学会, 農学進歩賞, 難吸収食品成分の機能性に関する時空間的制御機構の解明

  • Jan. 2019 Kobe university, 神戸大学 平成30年度優秀若手研究者賞, プロシアニジンの肥満高血糖予防効果

    ヤマシタヨウコ

    Others

  • Mar. 2018 日本農芸化学会, JSBBA Award for Young Women Scientists, プロシアニジンの高血糖・肥満予防効果に関する研究

    YAMASHITA YOKO

    Japan society

  • Dec. 2014 一般財団法人大阪科学技術センター, 第3回ネイチャー・インダストリー・アワード特別賞, メタボリックシンドロームの予防・改善に寄与する機能性成分の網羅的探索法と作用機構解明,ならびにそれを高含有する農作物生産のための環境創造型農業の開発

    YAMASHITA Yoko, ASHIDA HITOSHI, 保田 茂

    Japan society

  • Nov. 2014 日本フードファクター学会, Young Investigator Award 第1位, 若手研究者

    YAMASHITA YOKO

    Japan society

  • 2013 公益社団法人日本食品科学工学会, 第60回日本食品科学工学会 優秀発表賞, 重合度の異なるカカオ由来プロシアニジンが筋肉細胞でのGLUT4膜移行を介したグルコース取り込みにおよぼす効果

    YAMASHITA YOKO

    Japan society

  • 2013 日本薬学会生薬天然物部会, 第5回食品薬学シンポジウム 優秀発表賞, カカオポリフェノールの高血糖・肥満抑制効果とその作用機構

    YAMASHITA YOKO, ASHIDA HITOSHI

    Japan society

  • 2013 日本フードファクター学会, 第18回日本フードファクター学会 学術集会(JSoFF2013) Young investigator award銅賞, カカオ由来プロシアニジンによるインクレチン効果を介した血糖調整作用

    YAMASHITA YOKO

    Japan society

  • Sep. 2011 日本カテキン学会, 最優秀プレゼンテーション賞, 若手研究者

    YAMASHITA YOKO

    Japan society

  • 2011 日本フードファクター学会, JSoFF travel award, エピカテキン重合体であるプロシアニジンを高含有する組成物の肥満予防効果

    YOKO Yamashita

    International society

Published Papers

  • Yasukiyo Yoshioka, Qing Zhang, Xin Wang, Tomoya Kitakaze, Yoko Yamashita, Mitsutaka Kohno, Hitoshi Ashida

    Mung beans are among the important edible legumes cultivated in Asia, Southern Europe, and Northern America. Mung beans contain 20-30% proteins with high digestibility and possess biological activities, but detailed health beneficial functions are not fully understood yet. In this study, we report the isolation and identification of active peptides from mung beans which promote glucose uptake and elucidate their mechanism in L6 myotubes. HTL, FLSSTEAQQSY, and TLVNPDGRDSY were isolated and identified as active peptides. These peptides promoted the translocation of glucose transporter 4 (GLUT4) to the plasma membrane. The tripeptide HTL promoted glucose uptake through the activation of adenosine monophosphate-activated protein kinase, while the oligopeptides FLSSTEAQQSY and TLVNPDGRDSY through the activation of the PI3K/Akt pathway. Furthermore, these peptides promoted the phosphorylation of Jak2 via interaction with the leptin receptor. Thus, mung bean is a promising functional food for the prevention of hyperglycemia and type 2 diabetes through promoting glucose uptake accompanied by JAK2 activation in the muscle cells.

    23 May 2023, Food & function, English, International magazine

    Scientific journal

  • Kevin Odongo, Ken-Yu Hironao, Yoko Yamashita, Hitoshi Ashida

    Certain nutrients stimulate glucagon-like peptide-1 (GLP-1) secretion from the intestinal enteroendocrine L-cells, but due to rapid degradation by the DPP-4 enzyme, >90% is converted to inactive metabolite before reaching the target organs via circulation. Plants are a source of potent bioactive compounds that promote endogenous secretion of GLP-1 from L-cells. To search for the effective bioactive compound from a vast library of natural compounds, a reliable and low-cost assay is required considering the high cost of commercial assays. We developed a low-cost sandwich enzyme-linked immunosorbent assays (s-ELISAs) for detecting 'total', 'sensitive active', and 'wide-range active' GLP-1. The s-ELISAs exhibited high sensitivity with measurement ranges between 0.94~240, 0.98~62.5, and 4.8~4,480 pmol/L, respectively. High precision was observed; i.e., CVs within 5% and 20% for intra- and inter-assay variations, respectively, and excellent recovery of exogenous GLP-1 from assay buffer. The developed s-ELISAs had the same performance as the commercial kits and approximately 80% cheaper cost. For their application, cinnamtannin A2-induced GLP-1 secretion was confirmed in STC-1 cells consistent with our previous findings. The s-ELISAs were further validated by measuring plasma GLP-1 level in mice after oral administration of black soy bean seed coat extract containing cinnamtannin A2.

    Jan. 2023, Journal of clinical biochemistry and nutrition, 72 (1), 28 - 38, English, Domestic magazine

    Scientific journal

  • Mariko Shiraiwa, Tomoya Kitakaze, Yoko Yamashita, Yuichi Ukawa, Katsuyuki Mukai, Hitoshi Ashida

    Pectolinarigenin (PG) and its glycoside pectolinarin (PN) were reported to have various health beneficial functions such as anti-inflammatory and anti-carcinogenic activities. It has also been reported that PG and PN have radical scavenging ability as direct antioxidant activity. However, the indirect antioxidant activity of PG and PN by inducing antioxidant enzymes in hepatocytes is not fully understood yet. In this study, we investigated whether PG and PN increase expression of antioxidant enzymes through the nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated pathway in human hepatoma HepG2 cells and the liver of male ICR mice. PG, but not PN, induced antioxidant enzymes, namely heme oxigenase-1, NAD(P)H:quinone oxidoreductase 1, and aldo-keto reductase family 1 member B10, in HepG2 cells. As for the induction mechanism of these enzymes, PG-induced nuclear accumulation of Nrf2 increased antioxidant response element (ARE)-mediated transcriptional activity and suppressed degradation of Nrf2 through modification of Kelch-like EXH-associated protein 1. Oral administration of PG also induced nuclear accumulation Nrf2 and expression of antioxidant enzymes in the liver of mice. Therefore, PG, but not PN, exhibits the indirect antioxidant activity by inducing antioxidant enzymes through the Nrf2/ARE pathway and may protect liver from oxidative stress.

    30 Mar. 2022, Antioxidants (Basel, Switzerland), 11 (4), English, International magazine

    Scientific journal

  • Yasukiyo Yoshioka, Ryunoshin Kono, Masaki Kuse, Yoko Yamashita, Hitoshi Ashida

    Nutmeg is the seed of Myristica fragrans or its powder and is used as a spice and a traditional medicine. The antidiabetic effect of nutmeg is not fully understood yet. In this study, we examine the isolation and identification of the active compounds of Myristica fragrans with regards to glucose uptake and elucidate their mechanism in L6 myotubes. Myrisiticin, licarin B, erythro-2-(4-allyl-2,6-dimethoxy-phenoxy)-1-(3,4-dimethoxyphenyl)-propan-1-ol (ADDP) and (7S,8R)-2-(4-allyl-2,6-dimethoxyphenoxy)-1-(3,4,5-trimethoxyphenyl)-propan-1-ol (ADTP) were isolated and identified as the active compounds. Myristicin or a mixture of ADDP and ADTP promoted the translocation of glucose transporter 4 (GLUT4) through phosphorylation of AMP-activated protein kinase in L6 myotubes 15 min after treatment, while licarin B promoted it 240 min after treatment. Oral administration of the fraction from Myristica fragrans containing these active compounds to ICR mice suppressed post-prandial hyperglycemia. Thus, Myristica fragrans is a promising functional food to prevent post-prandial hyperglycemia and type 2 diabetes mellitus by promoting glucose uptake in muscle.

    11 Mar. 2022, Food & function, English, International magazine

    [Refereed]

    Scientific journal

  • Mio Yamamoto, Yasukiyo Yoshioka, Tomoya Kitakaze, Yoko Yamashita, Hitoshi Ashida

    Non-alcoholic fatty liver disease (NAFLD) and its advanced stage, non-alcoholic steatohepatitis (NASH), are a major health issue throughout the world. Certain food components such as polyphenols are expected to possess preventive effects on NAFLD and NASH. In this study, the preventive effects of black soybean polyphenols were examined by using three NAFLD/NASH animal models. In a choline-deficient and L-amino acid-defined high-fat diet-induced NASH model, the intake of black soybean polyphenols decreased oxidative stress, but failed in attenuating liver injury and decreasing the expression of alpha-smooth muscle actin (α-SMA). In a Western diet with sucrose and fructose containing sweetened water-induced NAFLD model, black soybean polyphenols suppressed hepatic lipid accumulation, oxidative stress, aminotransferase activities in the plasma, inflammatory cytokine expression, and α-SMA expression accompanied by modulation of lipid metabolism. In a combination of Western diet and carbon tetrachloride model, black soybean polyphenols also suppressed hepatic lipid accumulation, oxidative stress, aminotransferase activities in the plasma, and α-SMA expression. In conclusion, black soybean is an attractive food for the prevention of NAFLD and NASH due to its strong antioxidant activity.

    24 Jan. 2022, Food & function, 13 (2), 1000 - 1014, English, International magazine

    [Refereed]

    Scientific journal

  • Ken-Yu Hironao, Hitoshi Ashida, Yoko Yamashita

    High-fat diet (HFD) consumption induces chronic inflammation and microglial accumulation in the mediobasal hypothalamus (MBH), the central regulator of feeding behavior and peripheral metabolism. As a result, the diurnal feeding rhythm is disrupted, leading to the development of obesity. Diet-induced obesity (DIO) can be prevented by restoring the normal feeding pattern. Therefore, functional foods and drugs that ameliorate hypothalamic inflammation and restore the normal feeding pattern may prevent or ameliorate DIO. Numerous functional foods and food-derived compounds with anti-obesity effects have been identified; however, few studies have been performed that assessed their potential to prevent the HFD-induced hypothalamic inflammation and disruption of feeding rhythm. In the present study, we found that polyphenols derived from black soybean seed coat (BE) significantly ameliorated the accumulation of activated microglia and pro-inflammatory cytokine expression in the arcuate nucleus of the hypothalamus of HFD-fed mice, and restored their feeding pattern to one comparable to that of standard diet-fed mice, thereby ameliorating DIO. Furthermore, cyanidin 3-O-glucoside-the principal anthocyanin in BE-was found to be a strong candidate mediator of these effects. This is the first study to show that BE has the potential to provide a variety of beneficial effects on health, which involve amelioration of the HFD-induced hypothalamic inflammation and abnormal feeding pattern. The results of this study provide new evidence for the anti-obesity effects of black soybean polyphenols.

    2022, Frontiers in nutrition, 9, 1006132 - 1006132, English, International magazine

    Scientific journal

  • Yasushi Kuroda, Atsuko Kamiya, Takuya Ishii, Masahiro Ishizuka, Yoko Yamashita, Hitoshi Ashida

    Decreased mitochondrial metabolism suppresses glucose metabolism, resulting in obesity and diabetes. The present study aimed to investigate mechanisms underlying the 5-aminolevulinic acid (5-ALA) hydrochloride-mediated increase in glucose uptake in high-fat diet (HFD)-fed mice in vivo and C2C12 myotube cells in vitro. C57BL/6N male mice (20 weeks old) were fed either HFD or normal diet (ND) for 4 weeks. A total of five HFD-fed mice were orally administered with 300 mg/kg 5-ALA hydrochloride and 47.1 mg/kg sodium ferrous citrate (SFC; HFD + 5-ALA/SFC), whereas ND and other HFD-fed mice were orally administered with saline. After 4 weeks, these mice were intraperitoneally administered with 2 g/kg glucose and 3.2 mg/kg 2-deoxyglucose (2DG) for intraperitoneal glucose tolerance test (IPGTT) and glucose uptake test. Body weights, plasma glucose levels and the area under the curve of IPGTT were lower in mice treated with HFD + 5-ALA/SFC compared with in those treated with HFD alone. 2DG uptake in the gastrocnemius muscle and heart were more significantly improved in the HFD + 5-ALA/SFC mice compared with the HFD-fed mice. Furthermore, 5-ALA/SFC increased 2DG uptake in C2C12 cells to a similar level to the insulin-treated group. Moreover, it increased glucose transport (GLUT)1 translocation in the plasma membrane by 2.5-fold relative to the controls without affecting GLUT1 expression; however, it had no effect on GLUT4 translocation. Therefore, 5-ALA/SFC enhanced gastrocnemius and cardiac glucose uptake in HFD-fed mice, and upregulated GLUT1 translocation to the plasma membrane, but not GLUT4 in C2C12 myotube cells. Therefore, it could potentially be used as a novel drug for the treatment of diabetes.

    Dec. 2021, Experimental and therapeutic medicine, 22 (6), 1454 - 1454, English, International magazine

    [Refereed]

    Scientific journal

  • Tomoya Kitakaze, Hao Jiang, Takuya Nomura, Ken-Yu Hironao, Yoko Yamashita, Hitoshi Ashida

    Kaempferol possesses various health-promoting functions including antihyperglycemic activity, but its underlying molecular mechanism is poorly understood. Glucose transporter 4 (GLUT4) plays an important role in the uptake of blood glucose into muscle cells after its translocation to the plasma membrane. In this study, we demonstrated that kaempferol at 1.0 nM or more significantly increased the uptake of 2-[3H]- deoxy-d-glucose by 1.3-1.4-fold in L6 myotubes. Kaempferol at 10 pM or more also significantly increased GLUT4 translocation by 1.3-1.6-fold. Kaempferol at 1.0 nM significantly increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) by 2.9-fold, liver kinase B1 and Janus kinase 2 (JAK2) by 1.9-fold, and signal transducer and activator of transcription 3 by 3.7-fold. In addition, kaempferol increased phosphorylation of phosphoinositide 3-kinase (PI3K) by 1.8-fold but not the insulin receptor. Small interfering RNA (siRNA) for AMPK, JAK2, or PI3K canceled kaempferol-induced glucose uptake and GLUT4 translocation. Furthermore, siRNA for JAK2 canceled kaempferol-induced phosphorylation of AMPK and PI3K. These results indicate that a JAK2-depdendent pathway regulates kaempferol-induced glucose uptake and GLUT4 translocation in L6 myotubes and that kaempferol may be an effective compound for the prevention of hyperglycemia.

    25 Nov. 2020, Journal of agricultural and food chemistry, 68 (47), 13720 - 13729, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Hiroyuki Sakakibara, Toshiya Toda, Hitoshi Ashida

    Black soybean (Glycine max L.), a cultivar containing abundant polyphenols in its seed coat such as anthocyanins and flavan-3-ols, has been reported to possess various health benefits toward lifestyle diseases. In this review article, the safety evaluation of polyphenol-rich black soybean seed coat extract (BE) and absorption of BE polyphenols are summarized. Additionally, we describe the antioxidant activity of BE polyphenols and their ability to induce antioxidant enzymes. The health benefits of BE and its polyphenols, such as anti-obesity and anti-hyperglycemic activities through the activation of AMP-activated protein kinase and translocation of glucose transporter 4, respectively, are also discussed. Furthermore, we found that black soybean polyphenols were involved in the improvement of vascular function. These emerging data require further investigation in scientific studies and human trials to evaluate the prevention of lifestyle diseases using black soybean polyphenols.

    23 Sep. 2020, Food & function, 11 (9), 7321 - 7339, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Asuka Nakamura, Fumio Nanba, Shizuka Saito, Toshiya Toda, Junichi Nakagawa, Hitoshi Ashida

    Vascular dysfunction and injurious stimuli such as oxidative stress are closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols are reported to exert beneficial effects in reducing the risk of CVD. Black soybean has been used as a nutritionally rich food and contains abundant polyphenols in its seed coat and grain. Black soybean has many beneficial physiological activities, and its prevention effects on CVD risk were reported mainly in animal experiments. In this study, we performed a randomized, single blind, placebo controlled, crossover trial to investigate the effect of black soybean consumption on the vascular function in healthy humans. Twenty-two healthy adults aged from 30 to 60 completed the four week trial with daily consumption of about a 40 g test material cookie containing 20 g roasted black soybean powder. Body composition, vascular function, biomarkers for oxidative stress, and polyphenol contents in the urine and the plasma were measured. After ingestion of the black soybean cookie, vascular function, which was evaluated by plethysmogram using a Pulse Analyzer®, was improved and systolic blood pressure was decreased. Moreover, nitric oxide levels in plasma and urine were increased, while an oxidative stress biomarker, 8-hydroxy-2'-deoxyguanosine level, in the plasma was decreased accompanied by an increase in the concentration of polyphenols derived from black soybean in plasma and urine. These results suggest that the antioxidant activity of black soybean polyphenols and an increase in the nitric oxide level may contribute to the improvement of vascular function. Thus, black soybean is an attractive food material for improvement of vascular function through decreasing oxidative stress by its potent antioxidant activity and increasing the nitric oxide level in healthy humans.

    10 Sep. 2020, Nutrients, 12 (9), English, International magazine

    [Refereed]

    Scientific journal

  • Wataru Tanaka, Hiroki Matsuyama, Daigo Yokoyama, Yoko Yamashita, Hitoshi Ashida, Masanobu Sakono, Hiroyuki Sakakibara

    Elsevier BV, Sep. 2020, Journal of Functional Foods, 72, 104054 - 104054

    [Refereed]

    Scientific journal

  • Chiaki Domae, Hitoshi Ashida, Yoko Yamashita

    Background: Black soybean seed coat contains an abundance of flavan-3-ols and possesses various bioregulatory functions. Nitric oxide (NO) is produced by endothelial nitric oxide synthase (eNOS) in vascular endothelial cells and regulates vascular function through vasodilation and the inhibition of platelet aggregation in blood vessels. It has been reported that flavan-3-ols increase NO production, but many previous reports used a high concentration of flavan-3-ols. In the present study, we investigated the effect of flavan-3-ol-rich black soybean seed coat extract (BE) on NO production at a lower concentration that is close to the concentration after permeation through the monolayer of Caco-2 cells.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with BE, and then NO production in the medium and eNOS phosphorylation in the cells were examined. Intestinal epithelial Caco-2 cells on the upper side of a transwell filter were co-cultured with HUVEC on the basolateral compartment of the transwell apparatus. BE was added from the upper side, and the basolateral medium was collected to measure the concentration of NO and the content of flavan-3-ols. Furthermore, HUVEC were incubated with each flavan-3-ol in order to individuate the most effective compound in BE.Results: BE significantly increased NO production in the medium of HUVEC. When polyphenols in BE were removed from the basolateral medium by ethyl acetate extraction, increased NO production from HUVEC was not observed. Additionally, BE increased phosphorylation of eNOS and Akt in HUVEC. A portion of flavan-3-ols in BE had permeated through intestinal epithelial cells. Among the flavan-3-ols that had permeated, procyanidin C1 had the strongest effect on NO production in HUVEC at the concentration that had permeated the monolayer of Caco-2 cells. Procyanidin C1 (0.05 µM) also induced phosphorylation of eNOS and Akt in HUVEC without affecting the cAMP level. Conclusion: A portion of flavan-3-ols in BE directly promoted NO production through the Akt/eNOS pathway in vascular endothelial cells. These findings suggest that flavan-3-ols in the black soybean seed coat may contribute to improve the vascular function.Keywords: Black soybean seed coat polyphenols; NO; eNOS; Akt; vascular endothelial cells

    Functional Food Center, 13 Aug. 2020, Functional Foods in Health and Disease, 10 (8), 330 - 330

    Scientific journal

  • Yoko Yamashita, Liuqing Wang, Asuka Nakamura, Fumio Nanba, Shizuka Saito, Toshiya Toda, Junichi Nakagawa, Hitoshi Ashida

    Vascular dysfunction and injurious stimuli such as oxidative stress is closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols is reported to exert the beneficial effects on reducing the risk of CVD. Black soybean is rich in polyphenols, including isoflavones, anthocyanidins and flavan-3-ols, and its prevention effects on CVD risk were reported in the animal experiments. In this study, we investigated the effect of black soybean consumption on the vascular function and oxidative stress associating with the polyphenol concentrations in healthy women. Lowered vascular age was observed in 33 out of 44 volunteers who completed the 8-week trial. It was observed that improvement of the vascular stiffness, increasing in the urinary NO2 and NO3 level, and decreasing in the oxidative stress markers, 8-hydroxy-2'-deoxyguanosine, hexanoyl-lysine and myeloperoxidase. In addition, concentration of 12 polyphenols in black soybean increased in the plasma and urine. Increased concentration of polyphenols would be involved in the decreased oxidative stress. Thus, black soybean consumption improved the vascular function through an increase in nitric oxide and a decrease in oxidative stress accompanied by increasing the polyphenol concentrations in healthy women.

    30 Jul. 2020, Archives of biochemistry and biophysics, 688, 108408 - 108408, English, International magazine

    [Refereed]

    Scientific journal

  • Ken-Yu Hironao, Yuji Mitsuhashi, Shujiao Huang, Hideaki Oike, Hitoshi Ashida, Yoko Yamashita

    Energy metabolism and circadian rhythms are closely related together, i.e., the timing of nutrient intake affects metabolism under the regulation of circadian rhythms. Previously, we have reported that cacao liquor procyanidin (CLPr) promotes energy metabolism, resulting in preventing obesity and hyperglycemia. However, it is not unclear whether CLPr regulates clock gene expression. In this study, we investigated whether the administration timing of CLPr affected clock gene expression and found that CLPr regulated the circadian clock gene expression through the glucagon-like peptide-1 (GLP-1) signaling pathway. CLPr administration at Zeitgeber time 3 increased the expression level of Per family and Dbp in the liver. At the same administration timing, CLPr increased GLP-1 and insulin concentration in the plasma and phosphorylation of AMPK in the liver. It was noteworthy that an antagonist for GLP-1 receptor Exendin (9-39) canceled CLPr-increased expression of Per family and Dbp and phosphorylation of AMPK in the liver, in addition to insulin secretion. These results strongly suggest that CLPr-induced GLP-1 regulates the changes in clock gene expression in the liver through increased insulin. Thus, CLPr is a possible functional food material for prevention and/or amelioration of metabolic disorders through preventing circadian disruption through GLP-1 and AMPK pathways.

    Jul. 2020, Journal of clinical biochemistry and nutrition, 67 (1), 53 - 60, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Ken-Yu Hironao, Hitoshi Ashida, Yoko Yamashita

    Mammals have the biological clocks with approximately 24 h-rhythm. Energy metabolism including glucose metabolism is regulated by the biological clocks. Glucose metabolism is affected by not only meal volume and its energy but also meal timing. We have reported that cacao liquor procyanidin-rich extract (CLPr) ameliorated the postprandial hyperglycemia through AMP-activated protein kinase pathway. However, the effect of administration timing of CLPr on the postprandial hyperglycemia and its signaling pathway are still unclear. In the present study, we compared the effect of CLPr-administration at the rest-phase (light-period) and active-phase (dark-period) on glucose metabolism. Single oral administration of CLPr to ICR mice at the rest-phase, but not at the active-phase, promoted phosphorylation of AMP-activated protein kinase and its upstream liver kinase B1 and translocation of glucose transporter 4 to the plasma membrane in the skeletal muscle, resulting in reduced postprandial hyperglycemia. These results indicated that the intake of CLPr at the rest-phase more effectively suppressed postprandial hyperglycemia.

    Jul. 2020, Journal of clinical biochemistry and nutrition, 67 (1), 61 - 66, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Hitoshi Ashida, Xiaokuo Tian, Tomoya Kitakaze, Yoko Yamashita

    Turmeric and its components have various health beneficial functions. However, little is known about function of bisacurone, which is one of the sesquiterpenes in turmeric, at the compound level. In this study, we investigated the preventive effect of bisacurone on hepatic lipid accumulation and its mechanism in HepG2 cells and ICR mice. In HepG2 cells, bisacurone significantly inhibited fatty acid-induced intracellular lipid accumulation in a dose-dependent manner. Bisacurone at 10 µM increased protein expression of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1A accompanied by phosphorylation of AMP-activated protein kinase. In the liver of ICR mice, bisacurone decreased total lipids, triglyceride, and cholesterol contents. Bisacurone at 10 mg/kg body weight increased phosphorylation of AMP-activated protein kinase, and its downstream acetyl-CoA carboxylase as a rate-limiting enzyme for lipogenesis, while it decreased the nuclear translocation level of sterol regulatory element-binding protein 1 and carbohydrate-responsive element-binding protein as the major transcription factors for lipogenesis. On the other hand, bisacurone promoted lipolysis by up-expression of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1A. Thus, bisacurone might be a valuable food factor for preventing hepatic lipid accumulation by inhibiting lipogenesis and promoting lipolysis through phosphorylation of AMP-activated protein kinase.

    Jul. 2020, Journal of clinical biochemistry and nutrition, 67 (1), 43 - 52, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Hao Jiang, Yuko Horiuchi, Ken-Yu Hironao, Tomoya Kitakaze, Yoko Yamashita, Hitoshi Ashida

    Quercetin and its glycosides possess various health beneficial functions, but comparative study of them on energy metabolism in different tissues are not well studied. In this study, we investigated AMP-activated protein kinase regulated glucose metabolism in the skeletal muscle and lipid metabolism in the white adipose tissue and liver to compare the effectiveness of quercetin and its glycosides, namely isoquercitrin, rutin, and enzymatically modified isoquercitrin, in male ICR mice. The mice were fed a standard or high-fat diet supplemented with 0.1% quercetin and its glycosides for 13 weeks. Quercetin glycosides, but not quercetin, decreased body weight gain and fat accumulation in the mesenteric adipose tissue in high-fat groups. All compounds decreased high-fat diet-increased plasma glucose and insulin levels. Moreover, all compounds significantly increased AMP-activated protein kinase phosphorylation in either standard or high-fat diet-fed mice in all tissues tested. As its downstream events, all compounds induced glucose transporter 4 translocation in the muscle. In the white adipose tissue and liver, all compounds increased lipogenesis while decreased lipolysis. Moreover, all compounds increased browning markers and decreased differentiation markers in adipose tissue. Therefore, quercetin and its glycosides are promising food components for prevention of adiposity and hyperglycemia through modulating AMP-activated protein kinase-driven pathways.

    Jul. 2020, Journal of clinical biochemistry and nutrition, 67 (1), 74 - 83, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Yasukiyo Yoshioka, Yumi Samukawa, Yoko Yamashita, Hitoshi Ashida

    Since a decrease in muscle mass leads to an increased risk of mortality, the prevention of muscle wasting contributes to maintaining the quality of life. Recently, we reported that glabridin, a prenylated flavonoid in licorice, prevents dexamethasone-induced muscle loss. In this study, we focused on the other prenylated chalcones 4-hydroxyderricin and xanthoangelol in Ashitaba (Angelica keiskei) and investigated their prevention effect on dexamethasone-induced muscle loss. It was found that 4-hydroxyderricin and xanthoangelol significantly prevented dexamethasone-induced protein degradation in C2C12 myotubes by suppressing the expression of ubiquitin ligases, Cbl-b and MuRF-1. These prenylated chalcones acted as the antagonists of the glucocorticoid receptor and inhibited the binding of dexamethasone to this receptor and its subsequent nuclear translocation. In addition, the chalcones suppressed the phosphorylation of p38 and FoxO3a as the upstream factors for ubiquitin ligases. Dexamethasone-induced protein degradation and upregulation of Cbl-b were attenuated by the knockdown of the glucocorticoid receptor but not by the knockdown of p38. In male C57BL/6J mice, the Ashitaba extract, containing 4-hydroxyderricin and xanthoangelol, suppressed dexamethasone-induced muscle mass wasting accompanied by a decrease in the expression of ubiquitin ligases by inhibiting the nuclear translocation of the glucocorticoid receptor and phosphorylation of FoxO3a. In conclusion, 4-hydroxyderricin and xanthoangelol are effective compounds to inhibit steroid-induced muscle loss.

    24 Jun. 2020, Food & function, 11 (6), 5498 - 5512, English, International magazine

    [Refereed]

    Scientific journal

  • Tomoya Kitakaze, Sihao Yuan, Masako Inoue, Yasukiyo Yoshioka, Yoko Yamashita, Hitoshi Ashida

    In the body, alcohol dehydrogenase rapidly converts ethanol to its toxic metabolite, acetaldehyde, which is further metabolized to non-toxic acetic acid by aldehyde dehydrogenase (ALDH). 6-(methylsulfinyl)hexyl isothiocyanate (6-MSITC), a major bioactive compound in Wasabi (Wasabia japonica) has various physiological effects such as anti-oxidative, anti-inflammatory and anti-cancer effects. However, the effect of 6-MSITC on alcohol metabolism has not been studied. In this study, we investigated the effects of 6-MSITC on hepatic ALDH activity and protein expression both in vitro and in vivo. 6-MSITC inhibited ethanol- and acetaldehyde-induced cytotoxicity. Treatment with 6-MSITC to HepG2 cells enhanced ALDH activity through the induction of mitochondrial ALDH2 expression, but not cytosolic ALDH1A1. Knockdown of Nrf2 canceled the 6-MSITC-induced ALDH2 expression, indicating that Nrf2 regulated ALDH2 expression. Moreover, 6-MSITC increased the nuclear translocation of Nrf2 and the expression levels of HO-1 and SOD2, Nrf2-regulated phase II drug-metabolizing enzymes. Oral administration of 6-MSITC increased the mitochondrial ALDH2 activity and its expression in the liver of C57BL/6J mice. These results suggested that 6-MSITC is possible to protect acetaldehyde toxicity in hepatocytes by induction of mitochondrial ALDH2 expression through Nrf2/ARE pathway.

    15 Jun. 2020, Archives of biochemistry and biophysics, 686, 108329 - 108329, English, International magazine

    [Refereed]

    Scientific journal

  • Tomoya Kitakaze, Miki Yoshikawa, Yasuyuki Kobayashi, Naohiro Kimura, Naoki Goshima, Takahiro Ishikawa, Yoshiyuki Ogata, Yoko Yamashita, Hitoshi Ashida, Naoki Harada, Ryoichi Yamaji

    Skeletal muscle secretes biologically active proteins that contribute to muscle hypertrophy in response to either exercise or dietary intake. The identification of skeletal muscle-secreted proteins that induces hypertrophy can provide critical information regarding skeletal muscle health. Dietary provitamin A, β-carotene, induces hypertrophy of the soleus muscle in mice. Here, we hypothesized that skeletal muscle produces hypertrophy-inducible secretory proteins via dietary β-carotene. Knockdown of retinoic acid receptor (RAR) γ inhibited the β-carotene-induced increase soleus muscle mass in mice. Using RNA sequencing, bioinformatic analyses, and literature searching, we predicted transglutaminase 2 (TG2) to be an all-trans retinoic acid (ATRA)-induced secretory protein in cultured C2C12 myotubes. Tg2 mRNA expression increased in ATRA- or β-carotene-stimulated myotubes and in the soleus muscle of β-carotene-treated mice. Knockdown of RARγ inhibited β-carotene-increased mRNA expression of Tg2 in the soleus muscle. ATRA increased endogenous TG2 levels in conditioned medium from myotubes. Extracellular TG2 promoted the phosphorylation of Akt, mechanistic target of rapamycin (mTOR), and ribosomal p70 S6 kinase (p70S6K), and inhibitors of mTOR, phosphatidylinositol 3-kinase, and Src (rapamycin, LY294002, and Src I1, respectively) inhibited TG2-increased phosphorylation of mTOR and p70S6K. Furthermore, extracellular TG2 promoted protein synthesis and hypertrophy in myotubes. TG2 mutant lacking transglutaminase activity exerted the same effects as wild-type TG2. Knockdown of G protein-coupled receptor 56 (GPR56) inhibited the effects of TG2 on mTOR signaling, protein synthesis, and hypertrophy. These results indicated that TG2 expression was upregulated through ATRA-mediated RARγ and that extracellular TG2 induced myotube hypertrophy by activating mTOR signaling-mediated protein synthesis through GPR56, independent of transglutaminase activity.

    Feb. 2020, Biochimica et biophysica acta. Molecular cell research, 1867 (2), 118563 - 118563, English, International magazine

    [Refereed]

    Scientific journal

  • Tomoya Kitakaze, Atsushi Makiyama, Yoko Yamashita, Hitoshi Ashida

    A flavone luteolin has various health-promoting activities. Several studies reported that high dose of luteolin activates the Nrf2/ARE pathway in the liver. However, the effect of the low dose of luteolin that can be taken from a dietary meal on the Nrf2 activation remain unclear. It is expected that the flavonoid metabolism possesses a circadian rhythm, since nutritional metabolism processes daily cycle. In this study we investigated whether an administration affects the Nrf2 activation. ICR mice were orally administered 0.01-10 mg/kg body weight of luteolin once a day for 7 days at two time-points: at the start of active phase (ZT12) or at that of inactive phase (ZT0). Luteolin increased the nuclear translocation of Nrf2, resulting in the increases in its target gene products HO-1 and NQO1 at ZT12 but not at ZT0. The expression level of Nrf2 was lower at ZT12 than at ZT0 in the liver. We also found that the level of luteolin aglycon in the plasma is higher at ZT12 than at ZT0. These results suggest that the low dose of luteolin can activate Nrf2 pathway and the aglycon form of luteolin may mainly contribute to activate the Nrf2 pathway at ZT12 in the liver.

    2020, PloS one, 15 (4), e0231403, English, International magazine

    Scientific journal

  • Chiaki Domae, Fumio Nanba, Toshinari Maruo, Toshio Suzuki, Hitoshi Ashida, Yoko Yamashita

    Black soybean seed coat polyphenols were reported to possess various bioregulatory functions. However, the effects of black soybean seed coat polyphenols on vascular functions are unknown. Vascular dysfunction caused by aging and vascular stiffness is associated with a risk of cardiovascular disease (CVD), and a reduction in nitric oxide (NO) levels can trigger the onset of CVD. In the present study, we investigated the effect of polyphenol-rich black soybean seed coat extract (BE) on vascular functions and the underlying mechanisms involved. The oral administration of BE at 50 mg per kg body weight to Wistar rats increased NO levels as determined by eNOS phosphorylation. The administration of BE also increased GLP-1 and cAMP levels. Furthermore, the effects of BE were inhibited in the presence of a GLP-1 receptor antagonist. This suggests that GLP-1 is strongly involved in the underlying mechanism of NO production in vivo. In conclusion, BE contributes to the improvement of vascular functions by promoting NO production. Regarding the putative underlying mechanism, GLP-1 secreted from intestinal cells by the polyphenols in BE activates eNOS in vascular endothelial cells.

    11 Dec. 2019, Food & function, 10 (12), 7875 - 7882, English, International magazine

    [Refereed]

    Scientific journal

  • Takenaka Shinji, Yokoyama Moe, Kimura Yukihiro, Yamashita Yoko, Ashida Hitoshi

    Elsevier BV, Oct. 2019, PROCESS BIOCHEMISTRY, 85, 156 - 163

    [Refereed]

    Scientific journal

  • Hao Jiang, Yasukiyo Yoshioka, Sihao Yuan, Yuko Horiuchi, Yoko Yamashita, Kevin D Croft, Hitoshi Ashida

    Quercetin possesses various health beneficial functions, but its poor bioavailability limits these functions. Enzymatically modified isoquercitrin (EMIQ) is a quercetin glycoside with a greater bioavailability than quercetin. In this study, we investigated whether EMIQ regulates energy metabolism in mice and its underlying molecular mechanism. Male C57BL/6 mice were fed a normal diet with different doses of EMIQ or quercetin (0.02%, 0.1% and 0.5%) for two weeks. Supplementation with 0.1% EMIQ significantly decreased white adipose tissue (WAT) weight. Supplementation with 0.02% and 0.1% EMIQ promoted phosphorylation of adenosine monophosphate activated protein kinase (AMPK) in the WAT, liver, and muscle. In the WAT, 0.1% EMIQ downregulated peroxisome proliferator-activated receptor (PPAR)γ, CCAAT-enhancer-binding protein (C/EBP)α, C/EBPβ, and sterol regulatory element-binding protein 1 expression, as well as upregulated mitochondrial uncoupling protein (UCP) 2 and carnitine palmitoyltransferase-1 expression. Supplementation with 0.1% EMIQ also promoted the expression of thermogenesis-associated factors including PPARγ coactivator α (PGC-1α), UCP1, PR-domain containing protein 16, and sirtuin 1 in the WAT. In the liver, EMIQ promoted the phosphorylation of acetyl-CoA carboxylase, and increased the expression of PPARα, constitutive androstane-receptor, and farnesoid X receptor. Furthermore, supplementation with 0.02% or 0.1% EMIQ suppressed the plasma glucose level accompanied by the translocation of glucose transporter 4 to the plasma membrane of the muscle. Our results suggest that EMIQ is a potential food additive for the regulation of energy metabolism through AMPK phosphorylation.

    01 Aug. 2019, Food & function, 10 (8), 5188 - 5202, English, International magazine

    [Refereed]

    Scientific journal

  • Manabu Ueda-Wakagi, Hironobu Nagayasu, Yoko Yamashita, And Hitoshi Ashida

    It is known that green tea helps prevent obesity and diabetes mellitus. In this study, we aimed to determine whether green tea ameliorates hyperglycemia and the mechanism involved in diabetic rodents. Green tea consumption reduced blood glucose and ameliorated glucose intolerance, which was assessed using an oral glucose tolerance test in both streptozotocin-induced type 1 diabetic rats and type 2 diabetic KK-Ay mice. Green tea also reduced the plasma fructosamine and glycated hemoglobin concentrations in both models. Furthermore, it increased glucose uptake into the skeletal muscle of both model animals, which was accompanied by greater translocation of glucose transporter 4 (GLUT4). Moreover, epigallocatechin gallate (EGCG), the principal catechin in green tea, also ameliorated glucose intolerance in high-fat diet-induced obese and diabetic mice. These results suggest that green tea can ameliorate hyperglycemia in diabetic rodents by stimulating GLUT4-mediated glucose uptake in skeletal muscle, and that EGCG is one of the effective compounds that mediate this effect.

    16 May 2019, International journal of molecular sciences, 20 (10), English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Hideyuki Kishida, Kaku Nakagawa, Yasukiyo Yoshioka, Hitoshi Ashida

    For over 4000 years, liquorice has been one of the most frequently employed botanicals as a traditional herbal medicine. Although previous reports have found that liquorice flavonoids possess various health beneficial effects, the underlying mechanism responsible for the anti-diabetic effect of liquorice flavonoids remains unclear. The present study demonstrates that liquorice flavonoid oil (LFO) improves type 2 diabetes mellitus through GLUT4 translocation to the plasma membrane by activating both the adenosine monophosphate-activated protein kinase (AMPK) pathway and Akt pathway in muscle of KK-Ay mice. Furthermore, LFO lowered postprandial hyperglycaemia in a human study. These results indicate that LFO may exert a therapeutic effect on metabolic disorders, such as diabetes and hyperglycaemia, by modulating glucose metabolism through AMPK- and insulin-dependent pathways in skeletal muscle.

    May 2019, International journal of food sciences and nutrition, 70 (3), 294 - 302, English, International magazine

    [Refereed]

    Scientific journal

  • Yasukiyo Yoshioka, Yusuke Kubota, Yumi Samukawa, Yoko Yamashita, Hitoshi Ashida

    Prevention of muscle wasting is known to contribute to improving the quality of life and extending a healthy life. Recently, we have reported that licorice flavonoid oil containing glabridin, which is a prenylated isoflavone, enhances muscle mass in mice. In this study, we investigated the prevention effect of glabridin on dexamethasone-induced muscle atrophy and clarified its mechanism in cultured myotubes and in muscle of mice. Treatment with glabridin to C2C12 myotubes inhibited dexamethasone-induced protein degradation through dexamethasone-induced expression of ubiquitin ligases, MuRF1 and Cbl-b, but not atrogin-1. Mechanistically, glabridin inhibited nuclear translocation of the glucocorticoid receptor. Glabridin directly bound to the glucocorticoid receptor, resulting in the inhibition of binding between dexamethasone and the receptor protein. Glabridin also inhibited dexamethasone-induced phosphorylation of p38 and FoxO3a, as the upstream for the induction of ubiquitin ligases in C2C12 myotubes. Moreover, the glabridin-induced inhibition of protein degradation was eliminated by knockdown of the glucocorticoid receptor, but not by p38 knockdown. These data indicated that the inhibitory mechanism of glabridin against dexamethasone-induced muscle atrophy was mainly mediated by the inhibition of binding between dexamethasone and the glucocorticoid receptor in myotubes. Oral administration of glabridin prevented dexamethasone-induced protein degradation in the tibialis anterior muscle of mice. It was confirmed that glabridin inhibited dexamethasone-induced nuclear translocation of the glucocorticoid receptor and phosphorylation of FoxO3a in the muscle of mice. These findings suggest that glabridin is an effective food ingredient for the prevention of glucocorticoid-induced skeletal muscle atrophy.

    30 Mar. 2019, Archives of biochemistry and biophysics, 664, 157 - 166, English, International magazine

    [Refereed]

    Scientific journal

  • Tomoya Kitakaze, Atsushi Makiyama, Yumi Samukawa, Songyan Jiang, Yoko Yamashita, Hitoshi Ashida

    The flavon luteolin has various health-promoting activities including cardiovascular protection, anti-inflammatory activity and anticancer activity. A serum concentration of about 100 nM luteolin is reached by dietary habit. However, little is known about the function of luteolin over its physiological concentration range. In this study, we investigated whether a physiological concentration of luteolin could activate nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated expression of phase II drug-metabolizing enzymes in human hepatoma HepG2 cells. Interestingly, less than 1 nM of luteolin could induce phase II drug-metabolizing enzymes, such as GSTs, HO-1, and NQO1. Both 1 and 100 nM luteolin increased expression and activity of ALDH2, which metabolized toxic acetaldehyde into nontoxic acetic acid. Luteolin increased nuclear accumulation of Nrf2 and enhanced the ARE-binding complex through increasing the stability of the Nrf2 protein. Luteolin increased phosphorylation of Nrf2 at Ser40, and MEK inhibitors (U0126 and PD98059) canceled luteolin-induced phosphorylation of Nrf2. Furthermore, luteolin increased modified Keap1. In conclusion, a physiological concentration of luteolin induces the expression of phase II drug-metabolizing enzymes by enhancement of Nrf2 nuclear accumulation through MEK1/2-ERK1/2-mediated phosphorylation of Nrf2, increasing Nrf2 stability and inducing a conformational change of Keap1.

    15 Mar. 2019, Archives of biochemistry and biophysics, 663, 151 - 159, English, International magazine

    [Refereed]

    Scientific journal

  • Hao Jiang, Yoko Yamashita, Asuka Nakamura, Kevin Croft, Hitoshi Ashida

    Quercetin and its metabolite isorhamnetin elicit various beneficial effects on human health. However, their bioavailability is low. In this study, we investigated whether low concentrations in the physiological range could promote glucose uptake in L6 myotubes, as well as the underlying molecular mechanisms. We found that 0.1 nM and 1 nM quercetin or 1 nM isorhamnetin significantly increased glucose uptake via translocation of glucose transporter type 4 (GLUT4) to the plasma membrane of L6 myotubes. Quercetin principally activated the CaMKKβ/AMPK signalling pathway at these concentrations, but also activated IRS1/PI3K/Akt signalling at 10 nM. In contrast, 1 nM and 10 nM isorhamnetin principally activated the JAK/STAT pathway. Treatment with siAMPKα and siJAK2 abolished quercetin- and isorhamnetin-induced GLUT4 translocation, respectively. However, treatment with siJAK3 did not affect isorhamnetin-induced GLUT4 translocation, indicating that isorhamnetin induced GLUT4 translocation mainly through JAK2, but not JAK3, signalling. Thus, quercetin preferably activated the AMPK pathway and, accordingly, stimulated IRS1/PI3K/Akt signalling, while isorhamnetin activated the JAK2/STAT pathway. Furthermore, after oral administration of quercetin glycoside at 10 and 100 mg/kg body weight significantly induced GLUT4 translocation to the plasma membrane of skeletal muscles in mice. In the same animals, plasma concentrations of quercetin aglycone form were 4.95 and 6.80 nM, respectively. In conclusion, at low-concentration ranges, quercetin and isorhamnetin promote glucose uptake by increasing GLUT4 translocation via different signalling pathways in skeletal muscle cells; thus, these compounds may possess beneficial functions for maintaining glucose homeostasis by preventing hyperglycaemia at physiological concentrations.

    25 Feb. 2019, Scientific reports, 9 (1), 2690 - 2690, English, International magazine

    [Refereed]

    Scientific journal

  • Akiko Takagaki, Yasukiyo Yoshioka, Yoko Yamashita, Tomoya Nagano, Masaki Ikeda, Aya Hara-Terawaki, Ryota Seto, Hitoshi Ashida

    Glucose uptake ability into L6 skeletal muscle cell was examined with eleven kinds of ring fission metabolites of (-)-epigallocatechin gallate (EGCG) produced by intestinal bacteria. The metabolites 5-(3,5-dihydroxyphenyl)-γ-valerolactone (EGC-M5), 4-hydroxy-5-(3,4,5-trihydroxyphenyl)valeric acid (EGC-M6), 5-(3,4,5-trihydroxyphenyl)-γ-valerolactone (EGC-M7) and 5-(3-hydroxyphenyl)valeric acid (EGC-M11) have been found to promote uptake of glucose into L6 myotubes significantly. EGC-M5, which is one of the major ring fission metabolites of EGCG, was also found to have a promotive effect on glucose transporter 4 (GLUT4) translocation accompanied by phosphorylation of AMP-activated protein kinase (AMPK) signaling pathway in skeletal muscle both in vivo and in vitro. Furthermore, the effect of oral single dosage of EGC-M5 on glucose tolerance test with ICR mice was examined and significant suppression of hyperglycemia was observed. These data suggested that EGC-M5 has an antidiabetic effect in vivo.

    2019, Biological & pharmaceutical bulletin, 42 (2), 212 - 221, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

    Procyanidins have been reported to possess potential for the prevention of hyperglycaemia. However, there are very few data for procyanidins about the difference the degree of polymerisation (DP) has on anti-hyperglycaemic effects. Moreover, the underlying molecular mechanisms by which procyanidins suppress hyperglycaemia are not yet fully understood. In the present study, we prepared procyanidin fractions with different DP, namely low-DP (DP≤3) and high-DP (DP≥4) fractions, from a cacao liquor procyanidin-rich extract (CLPr). These fractions were administered orally to Institute of Cancer Research (ICR) mice and their anti-hyperglycaemic effects were examined. We found that CLPr and its fractions prevent postprandial hyperglycaemia accompanied by an increase in the plasma glucagon-like peptide-1 (GLP-1) level with or without glucose load. In the absence of glucose load, both fractions increased the plasma insulin level and activated its downstream signalling pathway in skeletal muscle, resulting in promotion of the translocation of GLUT4. Phosphorylation of AMP-activated protein kinase (AMPK) was also involved in the promotion of GLUT4 translocation. High- and low-DP fractions showed a similar activation of insulin and AMPK pathways. In conclusion, cacao liquor procyanidins prevent hyperglycaemia by promoting GLUT4 translocation in skeletal muscle, and both the GLP-1-activated insulin pathway and the AMPK pathway are involved in the underlying molecular mechanism.

    2019, Journal of nutritional science, 8, e2, English, International magazine

    [Refereed]

    Scientific journal

  • Liuqing Wang, Yoko Yamashita, Shingo Komeda, Akiko Saito, Hitoshi Ashida

    We investigated the absorption, metabolism, distribution and faecal excretion of 3 B-type procyanidin oligomers, including procyanidin B2, procyanidin C1 and cinnamtannin A2, and their monomeric unit (-)-epicatechin after a single oral administration of black soybean seed coat extract (BE) to male ICR mice at 250 mg per kg body weight. Plasma, tissues and faeces samples were collected within 24 h for the determination of (-)-epicatechin, procyandidin B2, procyanidin C1 and cinnamtannin A2 with or without β-glucuronidase and sulfatase treatment by the high-performance liquid chromatography method. A portion of the B-type procyanidin oligomers and (-)-epicatechin in BE was absorbed from the small intestine after the oral administration of BE. In the plasma, absorbed procyanidins and (-)-epicatechin existed mainly as conjugates. In the tissues, procyanidin B2, procyandin C1 and cinnamtannin A2, in addition to (-)-epicatechin distributed widely, primarily in their free forms. Their conjugation occurred mainly in the small intestine, rather than in the liver. Monomeric unit (-)-epicatechin had the highest bioavailability, followed by procyanidin B2, procyanidin C1 and cinnamtannin A2.

    17 Oct. 2018, Food & function, 9 (10), 5362 - 5370, English, International magazine

    [Refereed]

    Scientific journal

  • Yasukiyo Yoshioka, Yoko Yamashita, Hideyuki Kishida, Kaku Nakagawa, Hitoshi Ashida

    AIMS: Muscle mass is regulated by the balance between the synthesis and degradation of muscle proteins. Loss of skeletal muscle mass is associated with an increased risk of developing metabolic diseases such as obesity and type 2 diabetes mellitus. The aim of this study was to clarify the effects of licorice flavonoid oil on muscle mass in KK-Ay/Ta mice. MAIN METHODS: Male genetically type II diabetic KK-Ay/Ta mice received 0, 1, or 1.5 g/kg BW of licorice flavonoid oil by mouth once daily for 4 weeks. After 4 weeks, the femoral and soleus muscles were collected for western blotting for evaluation of the mTOR/p70 S6K, p38/FoxO3a, and Akt/FoxO3a signaling pathways. KEY FINDINGS: Ingestion of licorice flavonoid oil significantly enhanced femoral muscle mass without affecting body weight in KK-Ay/Ta mice. Licorice flavonoid oil also decreased expression of MuRF1 and atrogin-1, which are both markers of muscle atrophy. The mechanisms by which licorice flavonoid oil enhances muscle mass include activation of mTOR and p70 S6K, and regulation of phosphorylation of FoxO3a. SIGNIFICANCE: Ingestion of licorice flavonoids may help to prevent muscle atrophy.

    Elsevier Inc., 15 Jul. 2018, Life sciences, 205, 91 - 96, English, International magazine

    [Refereed]

    Scientific journal

  • Ayumi Maeda, Takeshi Shirao, Daishi Shirasaya, Yasukiyo Yoshioka, Yoko Yamashita, Mitsugu Akagawa, Hitoshi Ashida

    SCOPE: The prevalence of type 2 diabetes mellitus (T2DM) is increasing yearly worldwide. Glycemic control is the basis for the treatment of T2DM, as it can prevent the progress of associated complications. Spices possess various health beneficial effects on humans. The aim of this study is to search for spices that can promote glucose uptake and to elucidate the underlying molecular mechanism(s). METHODS AND RESULTS: Among 24 spice extracts, the extracts from black pepper and white pepper significantly increase glucose uptake in L6 myotubes. Piperine is found to be the active compound in these extracts. Treatment of myotubes with piperine induces the translocation of glucose transporter 4 (GLUT4) to the plasma membrane by phosphorylation of AMP-activated protein kinase (AMPK). Piperine increases the intracellular Ca2+ level and reactive oxygen species (ROS) generation through transient receptor potential vanilloid channel 1 (TRPV1), followed by activation of Ca2+ /calmodulin-dependent protein kinase kinase-beta (CaMKKβ) as the upstream events for AMPK phosphorylation. Furthermore, oral administration of piperine to Wistar rats at 0.01 and 0.1 mg kg-1 body weight decreases postprandial hyperglycemia accompanied by GLUT4 translocation and AMPK phosphorylation. CONCLUSION: Piperine in pepper prevents hyperglycemia by GLUT4 translocation through CaMKKβ/AMPK signaling via TRPV1-dependent increase in the intracellular Ca2+ level and ROS generation.

    Jun. 2018, Molecular nutrition & food research, 62 (11), e1800086, English, International magazine

    [Refereed]

    Scientific journal

  • Kevin D Croft, Yoko Yamashita, Helen O'Donoghue, Daishi Shirasaya, Natalie C Ward, Hitoshi Ashida

    The potential health benefits of phenolic acids found in food and beverages has been suggested from a number of large population studies. However, the mechanism of how these compounds may exert biological effects is less well established. It is also now recognised that many complex polyphenols in the diet are metabolised to simple phenolic acids which can be taken up in the circulation. In this paper a number of selected phenolic compounds have been tested for their bioactivity in two cell culture models. The expression and activity of endothelial nitric oxide synthase (eNOS) in human aortic endothelial cells and the uptake of glucose in muscle cells. Our data indicate that while none of the compounds tested had a significant effect on eNOS expression or activation in endothelial cells, several of the compounds increased glucose uptake in muscle cells. These compounds also enhanced the translocation of the glucose transporter GLUT4 to the plasma membrane, which may explain the observed increase in cellular glucose uptake. These results indicate that simple cell culture models may be useful to help understand the bioactivity of phenolic compounds in relation to cardiovascular protection.

    Apr. 2018, Fitoterapia, 126, 22 - 28, English, International magazine

    [Refereed]

    Scientific journal

  • Rika Nakai, Shun Fukuda, Masaya Kawase, Yoko Yamashita, Hitoshi Ashida

    Certain dioxins, including 2,3,7,8,-tetrachloro-dibenzo-p-dioxin (TCDD), are exogenous ligands for an aryl hydrocarbon receptor (AhR) and induces various drug-metabolizing enzymes. In this study, we examined the effect of curcumin on expression of drug-metabolizing enzymes through the AhR and NF-E2 related factor 2 (Nrf2) pathways. Curcumin dose-dependently inhibited TCDD-induced expression of phase I enzyme cytochrome P450 1A1 (CYP1A1) and phase II enzymes NAD(P)H:quinone oxidoreductase-1 (NQO1) and heme oxygenase 1 (HO-1) but not tert-butyl hydroquinone-induced NQO1 and HO-1, suggesting that curcumin inhibited only AhR pathway, but not Nrf2 one directly. Furthermore, we used 14 curcumin derivatives and obtained the correlation between hydrophobicity of the compounds and suppressive effect against AhR transformation. Results from the quantitative structure active correlative analysis indicated that methoxy groups and β-diketone structure possessing keto-enol tautomerism in curcumin were necessary to inhibit AhR transformation, and the addition of methyl and methoxy group(s) to the curcumin increased the inhibition effect.

    Japan Society for Bioscience Biotechnology and Agrochemistry, Apr. 2018, Bioscience, biotechnology, and biochemistry, 82 (4), 616 - 628, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Takakazu Mitani, Liuqing Wang, Hitoshi Ashida

    Cacao extract (CE) consumption has beneficial effects on human health, such as lowering the risk of obesity. However, the underlying molecular mechanism for the anti-obesity effect of CE remains incompletely understood. Here, we used a 50% aqueous alcohol extract of cacao mass, which is rich in methylxanthine derivatives (about 11%) and poor in flavan-3-ols (less than 1%), and assessed the suppression effects of this extract on adipocyte differentiation to investigate the anti-obesity mechanism. CE dose-dependently decreased fat accumulation in 3T3-L1 cells without affecting cell viability. CE also dose-dependently decreased the protein and gene expression levels of two adipogenesis-related transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs). Moreover, CE decreased protein expression levels of sterol regulatory element-binding protein 1 (SREBP1) and its downstream fatty acid synthase (FAS), which was accompanied by the retained localization of SREBP1 in the cytoplasm of 3T3-L1 cells. After ICR mice were fed a diet containing 1% CE for 1 wk, their white adipose tissue weight was lower, whereas their brown adipose tissue weight was higher compared with those of control animals. Additionally, the protein expression levels of PPARγ, C/EBPs, SREBP1, and FAS in the white adipose tissue of these mice were also lower than those in control animals. In contrast, diet supplementation with CE induced higher levels of phosphorylated AMP-activated protein kinase (AMPK) and its downstream acetyl-CoA carboxylase. In conclusion, methylxanthine derivative-rich CE decreases fat accumulation in adipocytes by downregulating the expression of the adipocyte differentiation master regulators through the activation of AMPK.

    Center for Academic Publications Japan, 2018, Journal of nutritional science and vitaminology, 64 (2), 151 - 160, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Mao Ito, Naoto Kudo, Yuji Miyake, Tatsuya Imai, Tomoki Unno, Yoko Yamashita, Yoshihisa Hirota, Hitoshi Ashida, Naomi Osakabe

    Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50mg/kg/day) for 2weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (-11.8%, -16.5%, and -41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (-5.3%, +2.0%, and -16.6%, respectively). The FL increased the EDL weight >20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.

    Nov. 2017, Experimental gerontology, 98, 120 - 123, English, International magazine

    [Refereed]

    Scientific journal

  • Salvatore Genovese, Hitoshi Ashida, Yoko Yamashita, Tomoya Nakgano, Masaki Ikeda, Shirasaya Daishi, Francesco Epifano, Vito Alessandro Taddeo, Serena Fiorito

    BACKGROUND: Glucose transporter 4 (GLUT4) is firmly established to play a pivotal role in glucose metabolism and in particular in modulating the insulin-stimulated glucose transport in several tissues, such as skeletal muscle and adipose tissue. Stimulation of GLUT4 by insulin results in its translocation to the plasma membrane, activation of several kinases, and finally in a large glucose influx into cells. PURPOSE: In this study we investigated the modulating properties of four biologically active oxyprenylated ferulic acid and umbelliferone derivatives and of their unprenylated parent compounds on GLUT-4 mediated glucose uptake and translocation. METHODS: Oxyprenylated phenylpropanoids have been synthesized in high yields and purity by already reported methodologies. All the synthesized chemicals were tested for their capacity to modulate GLUT4 mediated glucose uptake and GLUT4 translocation in L6 rat skeletal myoblasts in the concentration range 0.1 - 10 µM. Insulin (0.1 µM) was used as positive control. Western blot analysis was employed to assess if GLUT4 translocation occurred prior to increase of glucose uptake. Statistical analyses were carried out by the Dunnett multiple comparison test. RESULTS: 4'-Geranyloxyferulic acid (GOFA), 7-isopentenyloxycoumarin, and auraptene (7-geranyloxycoumarin) increased glucose uptake in a concentration-dependent manner, and significant increases were observed at 0.1 µM for GOFA, and 10 µM for 7-isopentenyloxycoumarin, and auraptene. These products also were able to significantly promote the translocation of GLUT4 to the plasma membrane of L6 myotubes. After treatment with compounds for 15 min, the incorporated amounts of GOFA, 7-isopentenyloxucoumarin, and auraptene were 0.15, 0.32, and 1.77 nmols/60-mm culture dish, respectively. A sample of raw Italian propolis, found to be rich in GOFA and auraptene, was also seen to mimic insulin-effect in the concentration range 0.01 - 1.0 mg/ml. CONCLUSIONS: Among the compounds assayed, auraptene showed to possess potentialities to be a potent activator of both translocation of GLUT4 and glucose influx into skeletal muscle cells with the highest bioavailability among effective compounds. Its capacity to modulate sugar metabolism, coupled to its presence in edible Citrus fruits, can be regarded as an additional reason to account for the already known stimulating properties of some vegetable (e.g. bitter orange).

    ELSEVIER GMBH, URBAN & FISCHER VERLAG, 15 Aug. 2017, Phytomedicine : international journal of phytotherapy and phytopharmacology, 32, 74 - 79, English, International magazine

    [Refereed]

    Scientific journal

  • Liuqing Wang, Yoko Yamashita, Akiko Saito, Hitoshi Ashida

    Procyanidins belong to a family of flavan-3-ols, which consist of monomers, (+)-catechin and (-)-epicatechin, and their oligomers and polymers, and are distributed in many plant-derived foods. Procyanidins are reported to have many beneficial physiological activities, such as antihypertensive and anticancer effects. However, the bioavailability of procyanidins is not well understood owing to a lack of convenient and high-sensitive analysis methods. The aim of this study was to develop an improved method for determining procyanidin content in both food materials and biological samples. High performance liquid chromatography (HPLC) coupled with a fluorescence detector was used in this study. The limits of detection (LODs) of (+)-catechin, (-)-epicatechin, procyanidin B2, procyanidin C1, and cinnamtannin A2 were 3.0×10-3 ng, 4.0×10-3 ng, 14.0×10-3 ng, 18.5×10-3 ng, and 23.0×10-3 ng, respectively; the limits of quantification (LOQs) were 10.0×10-3 ng, 29.0×10-3 ng, 28.5×10-3 ng, 54.1×10-3 ng, and 115.0×10-3 ng, respectively. The LOD and LOQ values indicated that the sensitivity of the fluorescence detector method was around 1000 times higher than that of conventional HPLC coupled with a UV-detector. We applied the developed method to measure procyanidins in black soybean seed coat extract (BE) prepared from soybeans grown under three different fertilization conditions, namely, conventional farming, basal manure application, and intertillage. The amount of flavan-3-ols in these BEs decreased in the order intertillage > basal manure application > conventional farming. Commercially available BE was orally administered to mice at a dose of 250 mg/kg body weight, and we measured the blood flavan-3-ol content. Data from plasma analysis indicated that up to the tetramer oligomerization, procyanidins were detectable and flavan-3-ols mainly existed in conjugated forms in the plasma. In conclusion, we developed a highly sensitive and convenient analytical method for the analysis of flavan-3-ols, and applied this technique to investigate the bioavailability of flavan-3-ols in biological samples and to measure flavan-3-ol content in food material and plants.

    FOOD & DRUG ADMINSTRATION, Jul. 2017, Journal of food and drug analysis, 25 (3), 478 - 487, English, International magazine

    [Refereed]

    Scientific journal

  • Takakazu Mitani, Yasukiyo Yoshioka, Takashi Furuyashiki, Yoko Yamashita, Yasuhito Shirai, Hitoshi Ashida

    Inflammatory bowel diseases are a group of chronic inflammation conditions of the gastrointestinal tract. Disruption of the mucosal immune response causes accumulation of oxidative stress, resulting in the induction of inflammatory bowel disease. In this study, we investigated the effect of enzymatically synthesized glycogen (ESG), which is produced from starch, on dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in C57BL/6 mice. Oral administration of ESG suppressed DSS- and TNBS-induced shortening of large intestine in female mice and significant decreased DSS-induced oxidative stress and TNBS-induced pro-inflammatory cytokine expression in the large intestine. ESG increase in the expression levels of heme oxygenase-1 (HO-1) and NF-E2-related factor-2 (Nrf2), a transcription factor for HO-1 expressed in the large intestine. Furthermore, ESG-induced HO-1 and Nrf2 were expressed mainly in intestinal macrophages. ESG is considered to be metabolized to resistant glycogen (RG) during digestion with α-amylase in vivo. In mouse macrophage RAW264.7 cells, RG, but not ESG decreased 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced reactive oxygen species (ROS). Knockdown of Nrf2 inhibited RG-induced HO-1 expression and negated the decrease in AAPH-induced ROS brought about by RG. RG up-regulated the protein stability of Nrf2 to decrease the formation of Nrf2-Keap1 complexes. RG-induced phosphorylation of Nrf2 at Ser40 was suppressed by ERK1/2 and JNK inhibitors. Our data indicate that ESG, digested with α-amylase to RG, suppresses DSS- and TNBS-induced colitis by increasing the expression of HO-1 in the large intestine of mice. Furthermore, we demonstrate that RG induces HO-1 expression by promoting phosphorylation of Nrf2 at Ser40 through activation of the ERK1/2 and JNK cascade in macrophages.

    ELSEVIER SCIENCE INC, May 2017, Free radical biology & medicine, 106, 355 - 367, English, International magazine

    [Refereed]

    Scientific journal

  • Yasukiyo Yoshioka, Xiu Li, Tianshun Zhang, Takakazu Mitani, Michiko Yasuda, Fumio Nanba, Toshiya Toda, Yoko Yamashita, Hitoshi Ashida

    Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (-)-epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages.

    JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, Mar. 2017, Journal of clinical biochemistry and nutrition, 60 (2), 108 - 114, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Takakazu Mitani, Tomoya Nagano, Kiyonari Harada, Yoko Yamashita, Hitoshi Ashida

    Caffeine is a methylxanthine derived from plant foods such as coffee beans and tea leaves, and has multiple biological activities against physiological response and several diseases. Although there are some reports about the direct effect of caffeine against anti-lipid accumulation in vitro, the effect of caffeine on lipid accumulation in adipocytes through stimulating intestinal epithelial cells is unknown. Since direct treatment with caffeine to 3T3-L1 cells did not affect lipid accumulation, we determined whether caffeine-stimulated intestinal epithelial Caco-2 cells influence the lipid accumulation in 3T3-L1 adipocytes. Caco-2 cells were cultured on a transwell insert with or without caffeine for 24 h. Subsequently, the basolateral component of the Caco-2 cell culture on the transwell was collected and termed caffeine-conditioning medium (CCM). When 3T3-L1 adipocytes were incubated with CCM, CCM decreased lipid accumulation and suppressed gene expression of proliferator activated receptor (PPAR) γ and CCAAT/enhancer binding protein (C/EBP) α in 3T3-L1 adipocytes. Furthermore, CCM decreased the expression of C/EBPβ and C/EBPδ at the protein level, but not at the mRNA level. We observed that a proteasome inhibitor, MG132, inhibited CCM-caused down-expression of C/EBPβ and C/EBPδ proteins, and that CCM promoted the ubiquitination level of C/EBPβ and C/EBPδ proteins. Protein microarray analysis showed caffeine suppresses the secretion of inflammatory cytokines, interleukin-8 and plasminogen activator inhibitor-1 from Caco-2 cells. These results suggest that caffeine indirectly suppresses lipid accumulation in 3T3-L1 adipocytes through decreasing secretion of inflammatory cytokines from Caco-2 cells.

    Center for Academic Publications Japan, 2017, Journal of nutritional science and vitaminology, 63 (5), 331 - 338, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Norio Yamamoto, Yoko Yamashita, Yasukiyo Yoshioka, Shin Nishiumi, Hitoshi Ashida

    Membrane proteins account for 70% to 80% of all pharmaceutical targets, indicating their clinical relevance and underscoring the importance of identifying differentially expressed membrane proteins that reflect distinct disease properties. The translocation of proteins from the bulk of the cytosol to the plasma membrane is a critical step in the transfer of information from membrane-embedded receptors or transporters to the cell interior. To understand how membrane proteins work, it is important to separate the membrane fraction of cells. This unit provides a protocol for rapidly obtaining plasma membrane fractions for western blot analysis. © 2016 by John Wiley & Sons, Inc.

    01 Aug. 2016, Current protocols in protein science, 85, 29.18.1-29.18.12 - 29.18.12, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Liuqing Wang, Fumio Nanba, Chiaki Ito, Toshiya Toda, Hitoshi Ashida

    Procyanidins are the oligomeric or polymeric forms of epicatechin and catechin. In this study, we isolated and purified dimer to tetramer procyanidins from black soybean seed coat and investigated the anti-hyperglycemic effects by focusing on glucose transporter 4 (GLUT4) translocation and the underlying molecular mechanism in skeletal muscle of mice. The anti-hyperglycemic effects of procyanidins were also compared with those of monomer (-)-epicatechin (EC) and major anthocyanin, cyanidin-3-O-β-glucoside (C3G). To investigate GLUT4 translocation and its related signaling pathways, ICR mice were orally given procyanidins, EC and C3G in water at 10 μg/kg body weight. The mice were sacrificed 60 min after the dose of polyphenols, and soleus muscle was extracted from the hind legs. The results showed that trimeric and tetrameric procyanidins activated both insulin- and AMPK-signaling pathways to induce GLUT4 translocation in muscle of ICR mice. We confirmed that procyanidins suppressed acute hyperglycemia with an oral glucose tolerance test in a dose-dependent manner. Of these beneficial effects, cinnamtannin A2, one of the tetramers, was the most effective. In conclusion, procyanidins, especially cinnamtannin A2, significantly ameliorate postprandial hyperglycemia at least in part by promoting GLUT4 translocation to the plasma membrane by activating both insulin- and AMPK-signaling pathways.

    PUBLIC LIBRARY SCIENCE, 2016, PloS one, 11 (9), e0161704, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Manabu Ueda-Wakagi, Mai Sakamoto, Nobuhiko Tachibana, Satoshi Wanezaki, Mitsutaka Kohno, Hitoshi Ashida

    Soy beta-conglycinin (beta CG) consumption has been shown to improve insulin resistance and suppress diet-induced obesity. These physiological effects seem to be mediated by the normalization of adiponectin and insulin sensitivity. Here, we show that artificial enzyme-hydrolyzed beta CG peptides promote uptake of 2-deoxyglucose, a glucose analogue, accompanied by translocation of glucose transporter 4 in skeletal L6 myotubes. Inhibition of AMP-activated protein kinase (AMPK) attenuated beta CG peptides-induced glucose uptake activity, while inhibition of both insulin and nitrogen monoxide signaling did not affect the glucose uptake activity. Taken together, these results indicate that beta CG peptides directly induced glucose uptake through the AMPK signaling pathway in muscle cells and might function to prevent insulin resistance.

    KARGER, Sep. 2015, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 21 (5), 727 - 732, English

    [Refereed]

    Scientific journal

  • Tomoya Nagano, Kaori Hayashibara, Manabu Ueda-Wakagi, Yoko Yamashita, Hitoshi Ashida

    We previously reported that the intake of black tea promotes translocation of the insulin-sensitive glucose transporter (GLUT) 4 in skeletal muscle. In this study, we investigated whether black tea polyphenols (BTP) promote GLUT4 translocation in L6 myotubes. BTP promoted glucose uptake accompanied by GLUT4 translocation in L6 myotubes. As the molecular mechanism, BTP induced the phosphorylation of insulin receptor substrate-1, atypical protein kinase C, Akt Thr308, Akt substrate 160, and AMP-activated protein kinase (AMPK), but did not affect that of Akt Ser473. BTP increased glycogen accumulation through inactivation of glycogen synthase kinase 3 beta (GSK-3 beta). Theaflavin, one of the major components in black tea, also promoted the glucose uptake accompanied by GLUT4 translocation observed with BTP in L6 myotubes. These results indicate that BTP activates both PI3K- and AMPK-dependent pathways to promote GLUT4 translocation and glycogen accumulation in skeletal muscle cells. Moreover, theaflavin is one of the active components in BTP.

    KARGER, May 2015, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 21 (3), 489 - 494, English

    [Refereed]

    Scientific journal

  • Hitoshi Ashida, Kiyonari Harada, Sakiho Mishima, Takakazu Mitani, Yoko Yamashita, Fumio Matsumura

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes various toxic effects, including wasting syndrome, through activation of an aryl hydrocarbon receptor (AhR). Our previous report demonstrated that certain flavonoids inhibit the activation of AhR and suppress its DNA binding activity. In this study, we searched for an active compound among 13 flavonoids that suppressed TCDD-induced loss of lipid accumulation using 3T3-L1 adipocytes as a cell culture model for wasting syndrome. Two flavonoids, luteolin and epigallocatechin gallate, suppressed TCDD-induced loss of lipid accumulation in this model. We further investigated luteolin to clarify the underlying molecular mechanism and confirmed that luteolin inhibited nuclear translocation of AhR caused by TCDD. Luteolin also inhibited the TCDD-driven decrease in protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). Although TCDD alone did not change protein expression of C/EBPβ and C/EBPδ, luteolin and TCDD up-regulated C/EBPδ expression in a dose-dependent manner. On the other hand, TCDD significantly decreased DNA binding of C/EBPβ and C/EBPδ, and luteolin completely canceled TCDD-decreased DNA binding of them. We conclude that luteolin suppresses the TCDD-induced loss of lipid accumulation in 3T3-L1 adipocytes by preventing a decrease in protein expression of PPARγ and C/EBPα, the master regulators of adipocyte differentiation and in DNA binding of C/EBPβ and C/EBPδ. Moreover, luteolin was rapidly incorporated and accumulated in 3T3-L1 adipocytes. Thus, luteolin is an attractive compound for the prevention of TCDD-induced wasting syndrome.

    ACADEMIC PRESS INC ELSEVIER SCIENCE, May 2015, Pesticide biochemistry and physiology, 120, 14 - 20, English, International magazine

    [Refereed]

    Scientific journal

  • Tianshun Zhang, Yoko Yamashita, Michiko Yasuda, Norio Yamamoto, Hitoshi Ashida

    Two main chalcones, 4-hydroxyderricin and xanthoangelol, from Ashitaba, which is a food ingredient and a folk medicine in Asia, have been demonstrated to modulate lipid metabolism in 3T3-L1 and HepG2 cells. In this study, we investigated the effects of Ashitaba extract on adiposity in mice fed a high-fat (HF) diet and its underlying mechanisms based on adipose tissue and hepatic lipid metabolism. C57BL/6 mice were fed a normal or HF diet supplemented with Ashitaba extract (0.01% and 0.1%, w/w) for 16 weeks. Ashitaba extract suppressed the HF diet-induced body weight gain and fat deposition in white adipose tissue, reduced plasma cholesterol, glucose, and insulin levels, increased the adiponectin level, lowered triglyceride and the liver cholesterol content, increased phosphorylation of AMP-activated protein kinase (AMPK) in adipose tissue and liver, inhibited lipogenesis in adipose tissue by down-expression of peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer-binding protein α and sterol regulatory element-binding protein 1 (SREBP1), inhibited lipogenesis in the liver by down-expression of SREBP1 and its target enzyme fatty acid synthase, and promoted fatty acid oxidation by up-expression of carnitine palmitoyltransferase-1A and PPARα. In conclusion, Ashitaba extract can possibly prevent adiposity through modulating lipid metabolism through phosphorylation of AMPK in adipose tissue and liver.

    ROYAL SOC CHEMISTRY, Jan. 2015, Food & function, 6 (1), 135 - 45, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Liuqing Wang, Lihua Wang, Yuki Tanaka, Tianshun Zhang, Hitoshi Ashida

    It is well known that tea has a variety of beneficial impacts on human health, including anti-obesity effects. It is well documented that green tea and its constituent catechins suppress obesity, but the effects of other types of tea on obesity and the potential mechanisms involved are not yet fully understood. In this study, we investigated the suppression of adiposity by oolong, black and pu-erh tea and characterized the underlying molecular mechanism in vivo. We found that the consumption of oolong, black or pu-erh tea for a period of one week significantly decreased visceral fat without affecting body weight in male ICR mice. On a mechanistic level, the consumption of tea enhanced the phosphorylation of AMP-activated protein kinase (AMPK) in white adipose tissue (WAT). This was accompanied by the induction of WAT protein levels of uncoupling protein 1 and insulin-like growth factor binding protein 1. Our results indicate that oolong, black and pu-erh tea, and in particular, black tea, suppresses adiposity via phosphorylation of the key metabolic regulator AMPK and increases browning of WAT.

    ROYAL SOC CHEMISTRY, Oct. 2014, Food & function, 5 (10), 2420 - 9, English, International magazine

    [Refereed]

    Scientific journal

  • Tianshun Zhang, Yuki Kimura, Songyan Jiang, Kiyonari Harada, Yoko Yamashita, Hitoshi Ashida

    UNLABELLED: Drugs, xenobiotics including environmental pollutants, and certain food components modulate expression of drug-metabolizing enzymes. An aryl hydrocarbon receptor (AhR) possesses possible expression of phase I and phase II enzymes directly by binding of its ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and indirectly by regulating expression of nuclear factor-erythroid-2-related factor 2 (Nrf2). Previous our result demonstrated that luteolin, a natural flavonoid existing in vegetables and herbs, competed the binding of TCDD to AhR. In the present study, we investigated the effect of luteolin on the expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways. Luteolin inhibited TCDD-induced protein expression of phase I enzyme cytochrome P450 1A1 (CYP1A1), phase II enzymes NAD(P)H: quinone oxidoreductase-1 (NQO1) and glutathione-S-transferase P1 (GSTP1) in HepG2, Hepa1c1c7 and RL-34 cells in a dose-dependent manner. Luteolin suppressed TCDD- and tert-butylhydroquinone-induced Nrf2 protein by decreasing its stability in HepG2 cells. In tert-butylhydroquinone treated cells, luteolin dose-dependently inhibited NQO1, GSTP1 and aldo-keto reductases (AKRs). Of these, protein expression of CYP1A1 and GSTP1 was mainly dominated by the AhR pathway, while that of NQO1 and AKRs was by the Nrf2 pathway. In conclusion, luteolin inhibits expression of phase I and phase II drug-metabolizing enzymes by modulating the AhR and Nrf2 pathways.

    ELSEVIER SCIENCE INC, 01 Sep. 2014, Archives of biochemistry and biophysics, 557, 36 - 46, English, International magazine

    [Refereed]

    Scientific journal

  • Keisuke Sawada, Yoko Yamashita, Tianshun Zhang, Kaku Nakagawa, Hitoshi Ashida

    The present study demonstrates that glabridin, a prenylated isoflavone in licorice, stimulates glucose uptake through the adenosine monophosphate-activated protein kinase (AMPK) pathway in L6 myotubes. Treatment with glabridin for 4h induced glucose uptake in a dose-dependent manner accompanied by the translocation of glucose transporter type 4 (GLUT4) to the plasma membrane. Glabridin needed at least 4h to increase glucose uptake, while it significantly decreased glycogen and increased lactic acid within 15 min. Pharmacological inhibition of AMPK by Compound C suppressed the glabridin-induced glucose uptake, whereas phosphoinositide 3-kinase and Akt inhibition by LY294002 and Akt1/2 inhibitor, respectively, did not. Furthermore, glabridin induced AMPK phosphorylation, and siRNA for AMPK completely abolished glabridin-induced glucose uptake. We confirmed that glabridin-rich licorice extract prevent glucose intolerance accompanied by the AMPK-dependent GLUT4 translocation in the plasma membrane of mice skeletal muscle. These results indicate that glabridin may possess a therapeutic effect on metabolic disorders, such as diabetes and hyperglycemia, by modulating glucose metabolism through AMPK in skeletal muscle cells.

    ELSEVIER IRELAND LTD, 05 Aug. 2014, Molecular and cellular endocrinology, 393 (1-2), 99 - 108, English, International magazine

    [Refereed]

    Scientific journal

  • Tianshun Zhang, Norio Yamamoto, Yoko Yamashita, Hitoshi Ashida

    AIM: We searched for polyphenols capable of inhibiting the lipid accumulation in 3T3-L1 cells, and investigated the mechanisms of two effective chalcones cardamonin and flavokawain B on differentiation of preadipocytes. METHOD AND RESULTS: We treated 3T3-L1 cells with a panel of 46 polyphenols and measured intracellular lipid accumulation by Sudan II staining. Four of them, including cardamonin and flavokawain B, inhibited lipid accumulation. In the further study, cardamonin and flavokawain B inhibited lipid accumulation by downregulating the expression of CCAAT/enhancer binding protein (C/EBP)-β, C/EBPα, and peroxisome proliferator-activated receptor-γ (PPARγ) at both mRNA and protein levels. Cardamonin and flavokawain B also increased phosphorylation of extracellular signal-regulated kinase (ERK) in the early phase of adipocyte differentiation. PD98059, an ERK inhibitor, restored C/EBPβ, PPARγ expression and intracellular lipid accumulation in adipocytes. Moreover, cardamonin and flavokawain B also modulated the secretion of C-reactive protein, dipeptidyl peptidase IV, interleukin-6, tumor necrosis factor-α and fibroblast growth factor-21 in mature adipocytes. CONCLUSIONS: These results indicate that ERK activation and consequent downregulation of adipocyte-specific transcription factors are involved in the inhibitory effects of the chalcones cardamonin and flavokawain B on adipocyte differentiation. Moreover, cardamonin and flavokawain B are able to modulate secretion of adipokines in mature adipocytes.

    ELSEVIER SCIENCE INC, 15 Jul. 2014, Archives of biochemistry and biophysics, 554, 44 - 54, English, International magazine

    [Refereed]

    Scientific journal

  • Nobuhiko Tachibana, Yoko Yamashita, Mayuko Nagata, Satoshi Wanezaki, Hitoshi Ashida, Fumihiko Horio, Mitsutaka Kohno

    Although the underlying mechanism is unclear, β-conglycinin (βCG), the major component of soy proteins, regulates blood glucose levels. Here, we hypothesized that consumption of βCG would normalize blood glucose levels by ameliorating insulin resistance and stimulating glucose uptake in skeletal muscles. To test our hypothesis, we investigated the antidiabetic action of βCG in spontaneously diabetic Goto-Kakizaki (GK) rats. Our results revealed that plasma adiponectin levels and adiponectin receptor 1 messenger RNA expression in skeletal muscle were higher in βCG-fed rats than in casein-fed rats. Phosphorylation of adenosine monophosphate-activated protein kinase (AMP kinase) but not phosphatidylinositol-3 kinase was activated in βCG-fed GK rats. Subsequently, βCG increased translocation of glucose transporter 4 to the plasma membrane. Unlike the results in skeletal muscle, the increase in adiponectin receptor 1 did not lead to AMP kinase activation in the liver of βCG-fed rats. The down-regulation of sterol regulatory element-binding factor 1, which is induced by low insulin levels, promoted the increase in hepatic insulin receptor substrate 2 expression. Based on these findings, we concluded that consumption of soy βCG improves glucose uptake in skeletal muscle via AMP kinase activation and ameliorates hepatic insulin resistance and that these actions may help normalize blood glucose levels in GK rats.

    PERGAMON-ELSEVIER SCIENCE LTD, Feb. 2014, Nutrition research (New York, N.Y.), 34 (2), 160 - 7, English, International magazine

    [Refereed]

    Scientific journal

  • Luteolin modulates expression of drug-metabolizing enzymes through AhR and Nrf2 pathways in hepatocytes

    KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, YAMASHITA Yoko, ASHIDA Hitoshi

    2014, Polyphenols Communications 2014 (Proceedings for XXVIth International Conference on Polyphenols & 8th Tannin Conference), 393 - 394, English

    International conference proceedings

  • Glabridin suppresses hyperglycemia through promotion of glucose uptake accompanied by translocation of glucose transporter 4 in muscle

    YAMASHITA Yoko, SAWADA Keisuke, KISHIDA Hideyuki, ZNAKAGAWA Kaku, ASHIDA Hitoshi

    2014, Polyphenols Communications 2014 (Proceedings for XXVIth International Conference on Polyphenols & 8th Tannin Conference), 361 - 362, English

    International conference proceedings

  • Fermented tea suppresses adiposity through the activation of AMPK and modulation of its downstream events.

    YAMASHITA Yoko, WANG Liuqing, WANG Lihua, TANAKA Yuki, ZHANG Tianshun, ASHIDA Hitoshi

    2014, Food and Functions, 5, 2420 - 2429, English

    [Refereed]

    Scientific journal

  • Effects of luteolin on TCDD- and tert-butylhydroquinone-induced drug-metabolizing enzynes and nuclear factor-erythroid-2-related factor 2.

    ASHIDA Hitoshi, ZHANG Tianshun, KIMURA Yuki, JIANG Songyan, HARADA Kiyonari, YAMASHITA Yoko

    2014, Organohalogen Compounds, 76, 317 - 320, English

    Scientific journal

  • Bioavailability of procyanidins in mice

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    2014, Polyphenols Communications 2014 (Proceedings for XXVIth International Conference on Polyphenols & 8th Tannin Conference), 589 - 590, English

    International conference proceedings

  • Masathito Takikawa, Aya Kumagai, Harumi Hirata, Minoru Soga, Yoko Yamashita, Manabu Ueda, Hitoshi Ashida, Takanori Tsuda

    SCOPE: 10-Hydroxy-2-decenoic acid (10H2DA) is one of the unique medium-chain fatty acids (MCFAs) specifically found in royal jelly. We hypothesize that 10H2DA has multiple biological functions and may aid in 5'-AMP-activated protein kinase (AMPK) activation and affect the glucose transport system in skeletal muscle. METHODS AND RESULTS: We examined whether various MCFAs present in royal jelly activated AMPKα. Treatment of L6 myotubes with various MCFAs showed that 10H2DA administration resulted in a significant increase in phosphorylated AMPKα. 10H2DA activates AMPK independently of insulin and significantly increased glucose uptake into L6 myotubes following translocation of glucose transporter 4 (Glut4) to the plasma membrane (PM). The activation was induced by the upstream kinase Ca²⁺/calmodulin-dependent kinase kinase β, but was independent of changes in AMP:ATP ratio and the liver kinase B1 pathway. Oral administration of 10H2DA significantly stimulated phosphorylation of AMPK and Glut4 translocation to the PM in mouse skeletal muscle. CONCLUSION: These findings indicate that (i) 10H2DA activates AMPK, and insulin independently enhances glucose uptake following translocation of Glut4 to PM, (ii) activation of AMPKα by 10H2DA is mediated via extracellular Ca²⁺-dependent Ca²⁺/calmodulin-dependent kinase kinase β, without alteration in the AMP:ATP ratio, and liver kinase B1 was not involved in the activation.

    WILEY-BLACKWELL, Oct. 2013, Molecular nutrition & food research, 57 (10), 1794 - 802, English, International magazine

    [Refereed]

    Scientific journal

  • Yuta Kurimoto, Yuki Shibayama, Seiya Inoue, Minoru Soga, Masahito Takikawa, Chiaki Ito, Fumio Nanba, Tadashi Yoshida, Yoko Yamashita, Hitoshi Ashida, Takanori Tsuda

    Black soybean seed coat has abundant levels of polyphenols such as anthocyanins (cyanidin 3-glucoside; C3G) and procyanidins (PCs). This study found that dietary black soybean seed coat extract (BE) ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase (AMPK) in type 2 diabetic mice. Dietary BE significantly reduced blood glucose levels and enhanced insulin sensitivity. AMPK was activated in the skeletal muscle and liver of diabetic mice fed BE. This activation was accompanied by the up-regulation of glucose transporter 4 in skeletal muscle and the down-regulation of gluconeogenesis in the liver. These changes resulted in improved hyperglycemia and insulin sensitivity in type 2 diabetic mice. In vitro studies using L6 myotubes showed that C3G and PCs significantly induced AMPK activation and enhanced glucose uptake into the cells.

    12 Jun. 2013, Journal of agricultural and food chemistry, 61 (23), 5558 - 64, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Masaru Yamaoka, Tomohisa Hasunuma, Hitoshi Ashida, Ken-ichi Yoshida

    Simple pharmacological studies on inositol stereoisomers are presented in this study. Male ICR mice were orally administered 1 g/kg BW of three inositol stereoisomers, myo-inositol (MI), d-chiro-inositol (DCI), and scyllo-inositol (SI), and blood plasma samples and skeletal muscle fractions were prepared after an hour. The plasma samples were subjected to gas chromatography-coupled time-of-flight mass spectrometry (GC-TOF-MS) analysis. None of the three stereoisomers was seen in untreated samples, but substantial amounts ranging from 2.5 to 6.5 mM were detected only after administration, indicating that orally administered inositol stereoisomers were readily absorbed and their levels elevated in the bloodstream. In addition, plasma of SI-administered animals contained substantial MI, suggesting a possible metabolic conversion of SI to MI. In the skeletal muscle fractions, glucose transporter type 4 (GLUT4) content in the plasma membrane increased, indicating that inositol stereoisomers stimulated GLUT4 translocation.

    22 May 2013, Journal of agricultural and food chemistry, 61 (20), 4850 - 4, English, International magazine

    [Refereed]

    Scientific journal

  • Tianshun Zhang, Songyan Jiang, Chao He, Yuki Kimura, Yoko Yamashita, Hitoshi Ashida

    Black soybean seed coat is a rich source of polyphenols that have been reported to have various physiological functions. The present study investigated the potential protective effects of polyphenolic extracts from black soybean seed coat on DNA damage in human hepatoma HepG2 cells and ICR mice. The results from micronucleus (MN) assay revealed that black soybean seed coat extract (BE) at concentrations up to 25μg/mL was non-genotoxic. It is noteworthy that BE (at 4.85μg/mL) and its main components, procyanidins (PCs) and cyanidin 3-glucoside (C3G), at 10μM significantly reduced the genotoxic effect induced by benzo[a]pyrene [B(a)P]. To obtain insights into the underlying mechanism, we investigated BE and its main components on drug-metabolizing enzyme expression. The results of this study demonstrate that BE and its main components, PCs and C3G, down-regulated B(a)P-induced cytochrome P4501A1 (CYP1A1) expression by inhibiting the transformation of aryl hydrocarbon receptor. Moreover, they increased expression of detoxifying defense enzymes, glutathione S-transferases (GSTs) via increasing the binding of nuclear factor-erythroid-2-related factor 2 to antioxidant response elements. Collectively, we found that PCs and C3G, which are the main active compounds of BE, down-regulated CYP1A1 and up-regulated GST expression to protect B(a)P-induced DNA damage in HepG2 cells and ICR mice effectively.

    ELSEVIER SCIENCE BV, 15 Apr. 2013, Mutation research, 752 (1-2), 34 - 41, English, International magazine

    [Refereed]

    Scientific journal

  • 大豆たん白質由来ペプチドによる筋肉細胞への糖取込み活性促進作用とその作用機序の解明

    SAWADA keisuke, YAMASHITA Yoko, ASHIDA Hitoshi

    不二たん白質研究振興財団, 2013, 大豆たん白質研究, 15 (33), 72 - 78, Japanese

    Symposium

  • Masaya Kawase, Tadashi Saito, Yukihiro Nakano, Yoko Yamashita, Sayuri Imada, Hitoshi Ashida

    To investigate the effect of catechins on Cu dynamics in mice, the body hair of mice administered with or without green tea extract was subjected to neutron activation analysis at a research reactor. Neutron activation analysis revealed that green tea extract might have the ability to promote excretion of Cu from the body to the hair.

    2013, Food Science and Technology Research, 19 (1), 123 - 125, English

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

    Procyanidins are oligomers and polymers of flavan-3-ols consisting of (-)-epicatechin subunits. In this study, we isolated and purified dimeric, trimeric and tetrameric procyanidins from cacao liquor and investigated their influence on the "incretin effect" as compared to the monomer, (-)-epicatechin in mice. Cinnamtannin A2 specifically increased the glucagon-like peptide-1 (GLP-1) and insulin secretion levels in the plasma after 60 min administration. As evidence of the action of insulin, activation of insulin receptor and insulin receptor substrate-1 was observed in the soleus muscle. These results indicate that the intake of cinnamtannin A2 may improve hyperglycemia through an incretin-like effect, accompanied by activation of the insulin-signaling pathway.

    2013, Bioscience, biotechnology, and biochemistry, 77 (4), 888 - 91, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

    In this study, we investigated whether cacao liquor procyanidin (CLPr) extract, which consists of 4.3% catechin, 6.1% epicatechin, 39.4% procyanidins and others, ameliorated hyperglycemia and obesity in C57BL/6 mice fed a control or high-fat diet for 13 weeks. CLPr suppressed high-fat diet-induced hyperglycemia, glucose intolerance and fat accumulation in white adipose tissue. CLPr also promoted translocation of glucose transporter 4 (GLUT4) and phosphorylation of AMP-activated protein kinase α (AMPKα) in the plasma membrane of skeletal muscle and brown adipose tissue. Phosphorylation of AMPKα was also enhanced in the liver and white adipose tissue. CLPr up-regulated the gene and protein expression levels of uncoupling protein (UCP)-1 in brown adipose tissue and UCP-3 in skeletal muscle. These results indicate that CLPr is a beneficial food material for the prevention of hyperglycemia and obesity. Activation of AMPKα, translocation of GLUT4 and up-regulation of UCP expression in skeletal muscle and adipose tissue are involved in the molecular mechanisms by which CLPr prevents hyperglycemia and obesity.

    ELSEVIER SCIENCE INC, 15 Nov. 2012, Archives of biochemistry and biophysics, 527 (2), 95 - 104, English, International magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Lihua Wang, Zhang Tinshun, Toshiyuki Nakamura, Hitoshi Ashida

    The antihyperglycemic effects of tea are well documented. However, the effects of fermented tea on the translocation of glucose transporter 4 (GLUT4), the major glucose transporter for glucose uptake in the postprandial period, in skeletal muscle and the underlying molecular mechanisms are not fully understood. This study investigated the translocation of GLUT4 and its related signaling pathways in skeletal muscle of male ICR mice given fermented tea. Intake of oolong, black, or pu-erh tea for 7 days enhanced GLUT4 translocation to the plasma membrane of skeletal muscle. Each type of fermented tea stimulated the phosphorylation of phosphoinositide 3-kinase (PI3K), Akt/protein kinase B, and AMP-activated protein kinase (AMPK). Fermented tea also increased the protein expression of insulin receptor. These results strongly suggest that fermented tea activates both PI3K/Akt- and AMPK-dependent signaling pathways to induce GLUT4 translocation and increases the expression of insulin receptor to improve glucose intolerance.

    AMER CHEMICAL SOC, 14 Nov. 2012, Journal of agricultural and food chemistry, 60 (45), 11366 - 71, English, International magazine

    [Refereed]

    Scientific journal

  • Comparison of Anti-Hyperglycemic Activities Between Low- and High-Degree of Polymerization Procyanidin Fractions from Cacao Liquor Extract

    Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

    Procyanidins are thought to be effective for prevention of hyperglycemia. To clarify the degree of polymerization (DP) of procyanidins on anti-hyperglycemic effects, we prepared low-DP (DP <= 3) and high-DP (DP >= 4) procyanidin fractions from cacao liquor, and examined whether these procyanidin fractions prevent hyperglycemia in vitro. We found that both fractions promoted glucose uptake in L6 myotubes in a dose-dependent manner accompanied by translocation of GLUT4 to the plasma membrane. Moreover, both fractions stimulate phosphorylation of AMP-activated protein kinase (AMPK). In these beneficial effects, low-DP fraction was more effective than high-DP one. On the other hand, high-DP fraction showed greater inhibitory effect of intestinal alpha-glucosidase activity than low-DP fraction in a cell free-incubation system. In conclusion, cacao liquor procyanidins have anti-hyperglycemic activities and low-DP procyanidins mainly contribute to stimulate glucose uptake by glucose transporter 4 translocation through AMPK-dependent pathway in skeletal muscle while high-DP procyanidins mainly contributes to inhibit the alpha-glucosidase activity in small intestine.

    BUREAU FOOD DRUG ANALYSIS, Apr. 2012, JOURNAL OF FOOD AND DRUG ANALYSIS, 20, 283 - 287, English

    [Refereed]

    Scientific journal

  • Coffee improves insulin-stimulated Akt phosphorylation in liver and skeletal muscle in diabetic KK-A(y) mice.

    Misato Kobayashi, Yuji Matsuda, Hiroshi Iwai, Masanori Hiramitsu, Takashi Inoue, Takao Katagiri, Yoko Yamashita, Hitoshi Ashida, Atsushi Murai, Fumihiko Horio

    Coffee has an anti-diabetic effect, specifically the amelioration of both hyperglycemia and insulin resistance, in KK-A(y) mice, a type 2 diabetes animal model. To investigate coffee's effect on insulin signaling in liver, skeletal muscle, and adipose tissue (epididymal fat), we assayed the tyrosine phosphorylation of insulin receptor (IR) and serine phosphorylation of Akt. In Expt. 1, we assayed insulin signaling under nonfasting conditions in KK-A(y) mice that ingested water or coffee for 4 wk. Coffee ingestion ameliorated the development of hyperglycemia but did not affect insulin signaling in liver or skeletal muscle under such conditions. In Expt. 2, we assayed insulin signaling under basal and insulin-stimulated conditions in KK-A(y) mice that ingested water or coffee for 3 wk. The levels of tyrosine phosphorylation of insulin receptor in response to insulin injection in insulin-sensitive tissues were not different between mice that drank water and those that drank coffee. Coffee ingestion significantly increased the insulin-induced serine phosphorylation of Akt in liver and skeletal muscle, but not in epididymal fat, of KK-A(y) mice. Our results also indicated that coffee ingestion may contribute to the improvement of insulin resistance and hyperglycemia in KK-A(y) mice via the activation of Akt in insulin signaling in liver and skeletal muscle.

    CENTER ACADEMIC PUBL JAPAN, 2012, Journal of nutritional science and vitaminology, 58 (6), 408 - 14, English, Domestic magazine

    [Refereed]

    Scientific journal

  • Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

    Hyperglycaemia and insulin resistance are associated with the increased risk of the metabolic syndrome and other severe health problems. The insulin-sensitive GLUT4 regulates glucose homoeostasis in skeletal muscle and adipose tissue. In this study, we investigated whether cacao liquor procyanidin (CLPr) extract, which contains epicatechin, catechin and other procyanidins, improves glucose tolerance by promoting GLUT4 translocation and enhances glucose uptake in muscle cells. Our results demonstrated that CLPr increased glucose uptake in a dose-dependent manner and promoted GLUT4 translocation to the plasma membrane of L6 myotubes. Oral administration of a single dose of CLPr suppressed the hyperglycaemic response after carbohydrate ingestion, which was accompanied by enhanced GLUT4 translocation in ICR mice. These effects of CLPr were independent of α-glucosidase inhibition in the small intestine. CLPr also promoted GLUT4 translocation in skeletal muscle of C57BL/6 mice fed a CLPr-supplemented diet for 7 d. These results indicate that CLPr is a beneficial food material for improvement of glucose tolerance by promoting GLUT4 translocation to the plasma membrane of skeletal muscle.

    2012, Journal of nutritional science, 1, e2 - 9, English, International magazine

    [Refereed]

    Scientific journal

  • Yuki Kanamoto, Yoko Yamashita, Fumio Nanba, Tadashi Yoshida, Takanori Tsuda, Itsuko Fukuda, Sachiko Nakamura-Tsuruta, Hitoshi Ashida

    Black soybean seed coat extract (BE) is a polyphenol-rich food material consisting of 9.2% cyanidin 3-glucoside, 6.2% catechins, 39.8% procyanidins, and others. This study demonstrated that BE ameliorated obesity and glucose intolerance by up-regulating uncoupling proteins (UCPs) and down-regulating inflammatory cytokines in C57BL/6 mice fed a control or high-fat diet containing BE for 14 weeks. BE suppressed fat accumulation in mesenteric adipose tissue, reduced the plasma glucose level, and enhanced insulin sensitivity in the high-fat diet-fed mice. The gene and protein expression levels of UCP-1 in brown adipose tissue and UCP-2 in white adipose tissue were up-regulated by BE. Moreover, the gene expression levels of major inflammatory cytokines, tumor necrosis factor-α and monocyte chemoattractant protein-1 were remarkably decreased by BE in white adipose tissue. BE is a beneficial food material for the prevention of obesity and diabetes by enhancing energy expenditure and suppressing inflammation.

    AMER CHEMICAL SOC, 24 Aug. 2011, Journal of agricultural and food chemistry, 59 (16), 8985 - 93, English, International magazine

    [Refereed]

    Scientific journal

MISC

  • 黒大豆ポリフェノールの機能性に関する研究.

    YAMASHITA Yoko

    2019, 化学と生物, 57, 146 - 152, Japanese

    [Refereed]

    Introduction scientific journal

  • 甘草由来グラブリジンの機能性について

    YAMASHITA YOKO, ASHIDA HITOAHI

    食品資材研究会, 2018, New food industry, 60, 1 - 7, Japanese

    [Invited]

    Introduction scientific journal

  • クロダイズの栽培と加工条件による種皮ポリフェノール含量と機能性の変化

    仲村 明日賀, YAMASHITA Yoko, 難波 文男, 戸田 登志也, ASHIDA Hitoshi

    2018, 日本ポリフェノール学会誌, 7, 32 - 40, Japanese

    [Refereed]

    Introduction other

  • ヒトの健康に寄与する食品成分探索、食物と健康

    ASHIDA Hitoshi, YAMASHITA Yoko

    2017, 神戸女子大学, 165, 3 - 5, Japanese

    [Invited]

    Introduction research institution

  • カカオポリフェノールのメタボリックシンドローム予防効果

    YAMASHITA Yoko, ASHIDA Hitoshi

    日本油化学会, 2017, オレオサイエンス, 17 (10), 467 - 474, Japanese

    [Refereed][Invited]

    Introduction scientific journal

  • YAMASHITA Yoko, ASHIDA Hitoshi

    公益社団法人 日本農芸化学会, 2016, 化学と生物, 54 (10), 747 - 752, Japanese

    [Refereed]

    Introduction scientific journal

  • 食品成分による耐糖の異常の改善について

    ASHIDA HITOSHI, 山下 陽子

    2015, 食品加工技術, 35 (1), 31 - 35, Japanese

    [Invited]

    Introduction scientific journal

  • プロシアニジンの新たな生体調節機能

    YAMASHITA Yoko, ASHIDA Hitoshi

    2014, 化学と生物, 52 (8), 493 - 494, Japanese

    [Refereed]

    Introduction scientific journal

  • 陽子先生の食育コラム第4回:食卓に真心を・・・

    YAMASHITA Yoko

    Jan. 2014, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成26年1月号, Japanese

    Introduction other

  • 陽子先生の食育コラム第3回:発酵食品に真心を・・・

    YAMASHITA Yoko

    Dec. 2013, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成25年12月号, Japanese

    Introduction other

  • 陽子先生の食育コラム第2回:”食育は真心”の主役は微生物 微生物に真心を・・・

    YAMASHITA Yoko

    Nov. 2013, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成25年11月号, Japanese

    Introduction other

  • 陽子先生の食育コラム第1回:食育は、”風土に根ざすまごころの営み”という視点を大切に・・・

    YAMASHITA Yoko

    Oct. 2013, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成25年10月号, Japanese

    Introduction other

  • フラボノイド類のグルコーストランスポーターを介した血糖値調節作用について

    YAMASHITA Yoko, ASHIDA Hitoshi

    2013, FFIジャーナル, 218, 158 - 165, Japanese

    Introduction commerce magazine

  • YAMASHITA Yoko, YAMAMOTO Norio, ASHIDA Hitoshi

    THE VITAMIN SOCIETY OF JAPAN, 2012, ビタミン学会誌, 86 (3), 163 - 173, Japanese

    [Refereed]

    Introduction scientific journal

Books etc

  • "Health beneficial functions of black soybean polyphenols" in "Food Biotechnology" 3rd Edition

    YAMASHITA Yoko, YOSHIOKA Yasykiyo, ASHIDA Hitoshi

    Joint work, CRC Press, 2019, English

    Scholarly book

  • 黒大豆ポリフェノール 「血流改善成分の開発と応用」

    YAMASHITA Yoko, ASHIDA Hitoshi

    Joint work, シーエムシー出版, 2018, Japanese

    Scholarly book

  • 第Ⅱ編食品素材としてのポリフェノールの研究開発;4.カカオポリフェノールの抗肥満効果『ポリフェノール: 『機能性成分研究開発の最新動向』

    ASHIDA Hitoshi, YAMASHITA Yoko

    Joint work, シーエムシー出版, 2016, Japanese

    Scholarly book

  • 第12章 プロシアニジンによる血糖ならびに脂質代謝調節「食品因子による栄養機能制御」pp. 177-195,日本栄養・食糧学会監修,芦田 均,立花 宏文,原 博 責任編集

    YOKO YAMASHITA, ASHIDA HITOSHI

    Joint work, 建帛社, May 2015, Japanese

    Scholarly book

Presentations

  • 生理的濃度域でのルテオリンの投与タイミングの違いによる薬物代謝酵素の発現について

    MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Nara, Domestic conference

    Oral presentation

  • レチノイン酸誘発性トランスグルタミナーゼ2は分泌タンパク質として筋肥大を誘発する

    YOSHIKAWA Miki, KITAKAZE Tomoya, HARADA Naoki, INUI Hiroshi, YAMASHITA Yoko, ASHIDA Hitoshi, YAMAJI Ryoichi

    第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Nara, Domestic conference

    Oral presentation

  • グラブリジンが筋萎縮予防におよぼす影響とその作用機構について

    YAMASHITA Yoko, YOSHIOKA Yasukiyo, KUBOTA Yusuke, ASHIDA Hitoshi

    第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Nara, Domestic conference

    Oral presentation

  • アシタバ由来カルコンによるステロイド性筋萎縮の抑制効果について

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Domestic conference

    Oral presentation

  • B-ring catechol structure of flavonol os important for promotion of glucose uptake into muscle cells

    AYA Megumi, SHIRASAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

    若手フロンティア研究会2018, Dec. 2018, Japanese, Kobe, Domestic conference

    Poster presentation

  • Ashidtaba chalcons prevent steroid-induced musle atrophy

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    若手フロンティア研究会2018, Dec. 2018, Japanese, Kobe, Domestic conference

    Poster presentation

  • The effect of black soybean on vascular function

    YAMASHITA Yoko, NAKAMURA Asuka, Asuka Nakamura, WANG Liuqing, SAITOU Shizuka, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi

    4th International Conference on Pharma and Food (ICPF2018), Nov. 2018, English, Shizuoka, International conference

    Poster presentation

  • Hepa1c1c7細胞における芳香族炭化水素受容体の活性化がBMAL1の発現に与える影響

    KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    第41回日本分子生物学会年会, Nov. 2018, Japanese, Yokohama, Domestic conference

    Poster presentation

  • Health beneficial functions of black soybean polyphenols

    ASHIDA Hitoshi, YAMASHITA Yoko

    4th International Conference on Pharma and Food (ICPF2018), Nov. 2018, English, Shizuoka, International conference

    [Invited]

    Invited oral presentation

  • Differences in polyphenol content of black soybeans among different varieties and cultivation years

    LI Weijing, YAMASHITA Yoko, ASHIDA Hitoshi

    JSBBA Kansai 5th Student Forum, Nov. 2018, English, Nara, International conference

    Poster presentation

  • B-ring catechol structure of flavonol is important for prevention of postprandial hyperglycemia via promotion of glucose uptake into muscle cells

    AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

    The31th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, Tsukuba, International conference

    Poster presentation

  • Ashiba chalcons prevent dexamethasone-induced muscle protein degradation

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    The31th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, Tsukuba, International conference

    Poster presentation

  • The preventive effect of Italian propolis on postprandial hyperglycemia

    SHIRASAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

    International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

    Poster presentation

  • The effect of black soybean on vascular function in human trial

    NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, SAITO Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

    Poster presentation

  • Preventive effect of black soybean polyphenols on non-alcoholic steatohepatitis

    YAMAMOTO Mio, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

    Poster presentation

  • Luteolin induces expression of phase II drug metabolizing enzymes by modulating Nrf2-ARE pathway in HepG2 cells

    KITAKAZE Tomoya, MAKIYAMA Atsushi, YAMASHITA Yoko, ASHIDA Hitoshi

    International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

    Poster presentation

  • Chalcones derived from ashitaba prevent dexamethasone-induced muscle protein degradation

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

    Poster presentation

  • 芳香族炭化水素受容体の活性化による時計遺伝子発現の変化に対する食品因子の作用

    KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • 生理的濃度域のルテオリンによるNrf2-ARE経路を介した薬物代謝酵素の発現調節機構

    MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    2018年度日本農芸化学会関西支部大会, Sep. 2018, Japanese, Kyoto, Domestic conference

    Oral presentation

  • 黒大豆ポリフェノール含量の品種並びに栽培年度での差について

    LI Weijing, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • 黒大豆ポリフェノールの血管機能向上効果とその作用機構の解明

    DOMAE Chiaki, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • 黒大豆ポリフェノールの吸収、代謝、分布と排泄および血管機能に及ぼす影響に関する研究

    WANG Liuqing, YAMASHITA Yoko, NAKAMURA Asuka, NAKAGAWA Junichi, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

    2018年度日本農芸化学会関西支部大会, Sep. 2018, Japanese, Kyoto, Domestic conference

    Oral presentation

  • 黒大豆クッキー摂取が欠陥機能に与える効果(ヒト介入試験)の検証

    NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, MIKI Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Oral presentation

  • ピペリンはTRPV1を介してAMPKを活性化し,グルコース取り込みを促進する

    MAEDA Ayumi, SHIRASAYA Daishi, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    2018年度日本農芸化学会関西支部大会, Sep. 2018, Japanese, Kyoto, Domestic conference

    Oral presentation

  • シカ肉の肝エネルギー代謝に関する研究

    TIAN Xiaokuo, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • クロダイズ種皮由来ポリフェノールによる脂肪蓄積抑制効果とその作用機序

    KOIKE Akihiro, YAMASHITA Yoko, ASHIDA Hitoshi, NAMBA Fumio, TODA Toshiya

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • クロダイズの非アルコール性脂肪肝炎予防効果について

    YAMAMOTO Mio, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • アシタバ由来カルコンによる筋萎縮抑制効果の検証とその分子機構の解明

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Oral presentation

  • Prevention mechanisms of glucose intolerance and obesity by enzymatically modified isoquercitrin and quercetin in mice

    JIANG Hao, YOSHIOKA Yasukiyo, YUAN Sihao, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, English, Kyoto, Domestic conference

    Oral presentation

  • Nrf2-ARE経路を介した薬物代謝酵素の発現調節機構に生理的濃度のルテオリンが与える影響

    MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • 健常者を対象とした黒大豆加工品摂取による血管機能向上効果(ヒト試験)の検証

    NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, MIKI Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    日本食品工学会第65回大会, Aug. 2018, Japanese, Sendai, Domestic conference

    Oral presentation

  • クロダイズが血管機能に及ぼす影響とその作用機構の解明

    DOMAE Chiaki, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    日本食品工学会第65回大会, Aug. 2018, Japanese, Sendai, Domestic conference

    Oral presentation

  • カカオポリフェノールが時計遺伝子の発現に及ぼす影響について

    YAMASHITA Yoko, HUANG shujiao, MITSUHASHI Yuji, ASHIDA Hitoshi

    第5回時間栄養科学研究会, Aug. 2018, Japanese, Saitama, Domestic conference

    Oral presentation

  • 芳香族炭化水素受容体の活性化と抑制作用をもつ食品成分による時計遺伝子の発現変動について

    KITAKAZE Tomoya, MAKIYAMA Atsushi, YAMASHITA Yoko, ASHIDA Hitoshi

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • 生理的濃度のルテオリンによるNrf2を介した薬物代謝酵素の発現調節機構

    MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    第71回日本酸化ストレス学会第18回日本NO学会合同学術集会, May 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • 黒大豆の血管機能向上効果とその作用機構

    DOMAE Chiaki, WANG Linqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    第71回日本酸化ストレス学会第18回日本NO学会合同学術集会, May 2018, Japanese, Kyoto, Domestic conference

    Poster presentation

  • 健常者を対象としたクロダイズ加工品摂取による血管機能向上効果(ヒト試験)の検証

    NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • フラボノールのB環カテコール構造が筋肉細胞へのグルコース取り込み促進を介した食後高血糖予防効果に重要である

    AYA Megumi, SHIRASAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • トランスグルタミナーゼ2は骨格筋のタンパク質合成を促進する

    YOSHIKAWA Miki, KITAKAZE Tomoya, HARADA Naoki, INUI Hiroshi, YAMASHITA Yoko, ASHIDA Hitoshi, YAMAJI Ryoichi

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • ケルセチンとその配糖体が血糖調節に及ぼす効果

    YAMASHITA Yoko, JIANG Hao, SHIRASAYA Daishi, AYA Megumi, ASHIDA Hitoshi

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • クロダイズ種皮由来ポリフェノールによる脂肪蓄積の抑制とその作用機構

    KOIKE Akihiro, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • クロダイズポリフェノールの非アルコール性脂肪肝疾患予防効果について

    YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • カカオポリフェノールの肥満・高血糖予防について

    ASHIDA Hitoshi, YAMASHITA Yoko

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    [Invited]

    Nominated symposium

  • アシタバカルコンによる筋タンパク質分解抑制効果の検証とその分子機構解明myotubes

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

    Oral presentation

  • 卵殻膜分解活性を示す微生物由来エラスターゼの検索と特性解析

    YOKOYAMA Moe, TAKENAKA Shinji, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • 生理的濃度のルテオリンはNrf2-Keap1経路を薬物代謝第II相酵素の発現量を調節する

    KITAKAZE Tomoya, MAKIYAMA Atsushi, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • 筋肉への糖取り込み促進効果を持つナツメグ抽出物中の関与成分の検討

    KONO Ryunoshin, MAEDA Ayumi, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • プロシアニジンの高血糖抑制効果とその作用機構についての比較検討

    YAMASHITA Yoko, TSUDA Takanori, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • フラボノールによるグルコース取り込み促進効果にはB環のカテコール構造が重要である

    AYA Megumi, SHIRSAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • ピペリンはROSを介してAMPKを活性化しグルコース取り込みを促進する

    MAEDA Ayumi, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • Kaempferol ameliorates glucose uptake via AMPK- and JAK-STAT-pathways in L6 myotubes

    JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

    Oral presentation

  • 野菜の機能性と健康効果

    YAMASHITA Yoko

    神河有機農業教室, Dec. 2017, Japanese, Hyogo, Domestic conference

    Public discourse

  • 腸内環境からみた黒大豆ポリフェノールによる健康長寿

    ASHIDA Hitoshi, YAMASHITA Yoko

    第15回日本機能性食品医用学会, Dec. 2017, Japanese, Tokyo, Domestic conference

    [Invited]

    Invited oral presentation

  • 食と健康に関する最新研究知見とそれを応用した食育の実践

    YAMASHITA Yoko

    中播磨地区学校給食研究協議会 栄養管理部会研修会, Dec. 2017, Japanese, Hyogo, Domestic conference

    Public discourse

  • β-カロテンが誘導する分泌型タンパク質は骨格筋のタンパク質合成を増加させる

    KITAKAZE Tomoya, HARADA Naoki, YAMASHITA Yoko, ASHIDA Hitoshi, YAMAJI Ryoichi

    第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

    Oral presentation

  • ルテオリンによるNrf2を介した薬物代謝酵素の誘導調節機構

    MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

    Oral presentation

  • プロポリス由来成分による高血糖予防効果の作用機序解明

    SHIRASAYA Daishi, IKADA Masaki, YAMASHITA Yoko, GENOVESE Salvatore, ASHIDA Hitoshi

    第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

    Oral presentation

  • ピペリンによるROSの産生を介したグルコース取り込み促進作用の解明

    MAEDA Ayumi, SHIRAO Takeshi, YOSHIOKA Yasykiyo, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

    第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

    Oral presentation

  • ナツメグ抽出物による筋肉への糖取り込み促進効果について

    KONO Ryunoshin, MAEDA Ayumi, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

    Oral presentation

  • アシタバカルコンによるデキサメタゾン誘導性筋タンパク質分解抑制効果に関する研究

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

    Oral presentation

  • 4-Hydroxyderricinによる筋タンパク質分解抑制効果の検証とその分子機構解明

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学若手フロンティア研究会2017, Dec. 2017, Japanese, Kobe, Domestic conference

    Poster presentation

  • 黒大豆種皮抽出物は肝臓の酸化ストレスを抑制する

    YAMAMOTO Mio, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

    Oral presentation

  • 黒大豆の機能性を再発見~環境創造型農業による黒大豆の研究~

    YAMASHITA Yoko

    たじま優しい有機農業講座, Nov. 2017, Japanese, Hyogo, Domestic conference

    Public discourse

  • 黒大豆の機能性を再発見~環境創造型農業による黒大豆の研究~

    YAMASHITA Yoko

    おおや有機農業の学校, Nov. 2017, Japanese, Hyogo, Domestic conference

    Public discourse

  • プロポリス由来成分による高血糖予防効果の解明

    SHIRASAYA Daishi, IKADA Masaki, NAGANO Tomoya, YAMASHITA Yoko, GENOVESE Salvatore, ASHIDA Hitoshi

    第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

    Oral presentation

  • ピペリンは ROS の産生を介してグルコース取り込みを促進する

    MAEDA Ayumi, SHIRAO Takeshi, YOSHIOKA Yasykiyo, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

    第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

    Oral presentation

  • アシタバカルコンによる筋タンパク質分解抑制効果に関する研究

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

    Oral presentation

  • The elucidating hyperglycemia preventive effect by propolis compounds

    SHIRASAYA Daishi, IKEDA Masaki, YAMASHITA Yoko, GENOVESE Salvatore, ASHIDA Hitoshi

    JSBBA Kansai 4th Student Forum, Nov. 2017, English, Kobe, Domestic conference

    Poster presentation

  • Cacao polypenols prevents hyperglycemia under the circadian clock genes

    HUANG Shujiao, YAMASHITA Yoko, ASHIDA Hitoshi

    JSBBA Kansai 4th Student Forum, Nov. 2017, English, Kobe, Domestic conference

    Poster presentation

  • Procyanidin prevents hyperglycemia through promoting translocation of glucose transporter 4 in muscle cells

    YAMASHITA Yoko, ASHIDA Hitoshi

    8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

    Oral presentation

  • Glabridin inhibits glucocorticoid-induced muscle atrophy in C2C12 cells and C57BL6J mice

    ASHIDA Hitoshi, KUBOTA Yusuke, YOSHIOKA Yasukiyo, YAMASHITA Yoko

    8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

    Oral presentation

  • Fisetin, but not robinetin, promotes glucose uptake in skeletal muscle cells

    AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

    8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

    Oral presentation

  • Enzymatically Synthesized Glycogen Inhibits Colitis Through Decreasing Oxidative Stress

    ASHIDA Hitoshi, MITANI Takakazu, YOSHIOKA Yasukiyo, YAMASHITA Yoko, FURUYASIKI Takashi

    The 10th International Conference and Exhibition on Nutraceuticals and Functional Foods (ISNFF2017),, Oct. 2017, English, Gunsan, International conference

    [Invited]

    Invited oral presentation

  • Curcumin and its derivatives inhibit TCDD-induced expression of drug metabolizing enzymes

    ASHIDA Hitoshi, NAKAI Rika, YAMASHITA Yoko

    8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

    Poster presentation

  • Black soybean seed coat polyphenols prevent fibrosis in the liver of mice via suppression of lipid peroxidation and inhibition of TGF-b Smads signaling pathway

    YOSHIOKA Yasukiyo, LI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

    8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

    Poster presentation

  • 発酵茶が脂肪蓄積と高血糖予防に及ぼす効果とその作用機構

    YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会(第49回講演会), Sep. 2017, Japanese, Sakai, Domestic conference

    Oral presentation

  • 食と健康に関する最新研究知見とそれを応用した食育の実践

    YAMASHITA Yoko

    日本教職員組合栄養教職員部近畿ブロック学習会, Sep. 2017, Japanese, Hyogo, Domestic conference

    Public discourse

  • 黒ダイズ種皮抽出物は四塩化炭素の誘導する肝の線維化を抑制する

    YOSHIOKA Yasukiyo, RI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

    フードサイエンスフォーラム第23回学術集会, Sep. 2017, Japanese, Miyazaki, Domestic conference

    Oral presentation

  • マウスにおけるプロシアニジンの体内動態

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会(第49回講演会), Sep. 2017, Japanese, Sakai, Domestic conference

    Oral presentation

  • ヒト介入試験による黒大豆の血管機能向上効果について

    YAMASHITA Yoko, ASHIDA Hitoshi

    フードサイエンスフォーラム第23回学術集会, Sep. 2017, Japanese, Miyazaki, Domestic conference

    Oral presentation

  • アシタバカルコン4-hydroxyderricinはデキサメタゾン誘導性の筋タンパク質分解を抑制する

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    フードサイエンスフォーラム第23回学術集会, Sep. 2017, Japanese, Miyazaki, Domestic conference

    Oral presentation

  • 煎り黒豆を継続摂取したヒト血中および尿中のポリフェノール濃度.

    WANG Liuqing, YAMASHITA Yoko, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

    日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

    Oral presentation

  • 煎り黒大豆摂取がヒトの血管機能向上に及ぼす効果

    YAMASHITA Yoko, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

    日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

    Oral presentation

  • 煎り黒大豆の摂取によるヒト血管機能の向上

    ASHIDA Hitoshi, YAMASHITA Yoko, NAMBA Fumio, TODA Toshiya

    SIP「次世代機能性農林水産物・食品の開発」報告会, Aug. 2017, Japanese, Tokyo, Domestic conference

    [Invited]

    Nominated symposium

  • 黒大豆種皮抽出物の血管内皮機能改善作用

    SAITOU Shizuka, NAMBA Fumio, ASHIDA Hitoshi, YAMASHITA Yoko, TODA Toshiya

    日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

    Oral presentation

  • 黒大豆ポリフェノールによる非アルコール性脂肪肝疾患の予防効果と作用機構の解明

    YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • 高血糖予防効果を有する緑豆由来ペプチドの探索

    YOSHIOKA Yasukiyo, WANG Xin, KOHNA Mitsutaka, TACHIBANA Nobuhiko, YAMASHITA Yoko, ASHIDA Hitoshi

    日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

    Oral presentation

  • 加工過程によるクロダイズポリフェノール成分の変化とその生体機能性について

    NAKAMURA Asuka, LIUQING Wang, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • プロポリス由来prenyloxyphenylpropanoidsによる糖取り込み促進作用の作用機序解明

    SHIRASAYA Daishi, NAGANO Tomoya, IKEDA Masaki, YAMASHITA Yoko, Salvatore Genovese, ASHIDA Hitoshi

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • フィセチンとロビネチンの筋肉細胞への取り込み量と糖取り込み促進効果の関係について

    AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • クロダイズ種皮由来ポリフェノールによる高血糖・肥満抑制効果とその作用機構

    KOIKE Akihiro, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • カカオポリフェノール抽出物の投与タイミングの違いによる血糖調節と時計遺伝子に及ぼす影響

    HUANG shujiao, MITSUHASHI Yuji, ASHIDA Hitoshi, YAMASHITA Yoko

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • カカオポリフェノール抽出物の投与タイミングの違いが高血糖抑制作用と時計遺伝子に及ぼす影響

    HUANG Shujiao, MITSUHASHI Yuji, ASHIDA Hitoshi, YAMASHITA Yoko

    日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

    Oral presentation

  • アシタバカルコン 4-hydroxyderricinによる筋萎縮抑制効果について

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

    Oral presentation

  • The effect of kaempferol on glucose uptake in L6 myotubes

    JIANG Hao, YOSHIOKA Yasukiyo, Yoko Yamashita, Hitoshi Ashida

    日本食品科学工学会 第64回大会, Aug. 2017, English, Syonan, Domestic conference

    Oral presentation

  • ルテオリンによるNrf2を介した薬物代謝酵素の誘導調節

    MAKIYAMA Atsushi, JIANG Songyan, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

    Poster presentation

  • プロポリス由来成分による筋肉細胞への糖取り込み作用について

    SHIRASAYA Daishi, NAGANO Tomoya, IKEDA Masaki, YAMASHITA Yoko, Salvatore Genovese, ASHIDA Hitoshi

    日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

    Poster presentation

  • フィセチンとロビネチンによる筋肉細胞の糖取り込み促進効果について

    AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

    日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

    Poster presentation

  • アシタバカルコン4-hydroxyderricinによる筋萎縮抑制効果について

    SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

    日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

    Poster presentation

  • The effect of kaempferol on glucose uptake in L6 myotubes

    JIANG Hao, YOSHIOKA Yasukiyo, Yoko Yamashita, Hitoshi Ashida

    日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, English, Matsuyama, Domestic conference

    Poster presentation

  • 黒大豆種皮由来ポリフェノールによる肝線維化抑制効果

    YOSHIOKA Yasukiyo, RI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

    第70回日本酸化ストレス学会学術集会, Jun. 2017, Japanese, Tsukuba, Domestic conference

    Oral presentation

  • FisetinとRobinetinによる筋肉細胞のGLUT4膜移行促進効果の差異について

    AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

    日本ビタミン学会第69回大会, Jun. 2017, Japanese, Yokohama, Domestic conference

    Oral presentation

  • 黒大豆種皮ポリフェノールはTGFβ1の誘導する肝線維化を阻害する

    YOSHIOKA Yasukiyo, NAMBA Fumio, TODA Toshiya, YAMASHITA Yoko, ASHIDA Hitoshi

    第71回日本栄養・食糧学会, May 2017, Japanese, Okinawa, Domestic conference

    Oral presentation

  • 黒大豆の機能性を再発見~環境創造型農業による黒大豆の研究~

    YAMASHITA Yoko, ASHIDA Hitoshi

    神付有機農業教室, May 2017, Japanese, Hyogo, Domestic conference

    Public discourse

  • グラブリジンの高血糖抑制効果について

    YAMASHITA Yoko, NAKAGAWA Kaku, KISHIDA Hideyuki, ASHIDA Hitoshi

    第71回日本栄養・食糧学会, May 2017, Japanese, Okinawa, Domestic conference

    Oral presentation

  • 黒大豆種皮抽出物の血管内皮機能改善作用

    NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • 黒大豆の生体機能性について

    YAMASHITA Yoko, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • 黒大豆の加工によるポリフェノール含量と機能性の変化

    NAKAMURA Asuka, Liuqing Wang, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • ナツメグ抽出物による筋肉への糖取り込み促進効果について

    KONO Ryunoshin, MAEDA Ayumi, YOSHIOKA Yasukiyo, IKEDA Masaki, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • ケンフェロールとルテオリンによる薬物代謝酵素発現調節機構について

    NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • カカオポリフェノールによるエネルギー代謝促進効果:投与タイミングと時計遺伝子発現

    MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • アシタバカルコンはAMPK経路を介して成熟白色脂肪細胞のベージュ化を促進する

    LiuYiyun, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

    Oral presentation

  • 大豆の機能性を再発見~食による健康都市づくり~

    YAMASHITA Yoko

    平成28年度近畿産大豆生産・需要拡大協議会講演会, Feb. 2017, Japanese, Kyoto, Domestic conference

    Public discourse

  • 大学における機能性食品に関する研究と健康都市づくり事業について

    YAMASHITA Yoko

    日本食品保健指導士会 関西支部平成28年度研修会, Feb. 2017, Japanese, Oosaka, Domestic conference

    Public discourse

  • クルクミンおよびその誘導体による薬物代謝酵素発現調節機構の解明に関する研究(

    NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会関西支部第497回講演会, Dec. 2016, Japanese, Kobe, Domestic conference

    Oral presentation

  • 脂質・エネルギー代謝と体内時計との関係 〜いつ・何をたべる?〜

    YAMASHITA Yoko

    第24回日本植物油協会植物油栄養懇話会, Nov. 2016, Japanese, Tokyo, Domestic conference

    [Invited]

    Invited oral presentation

  • カカオポリフェノールの代謝促進効果:投与タイミングと時計遺伝子

    MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

    第21回フードファクター学会学術集会, Nov. 2016, Japanese, Toyama, Domestic conference

    Poster presentation

  • Xanthoangelol promotes browning in white adipocyte through activation of AMP-activated protein kinase

    LiuYiyun, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

    The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

    Poster presentation

  • The effect of quercetin and its metabolite isorhamnetin on glucose uptake in myotubes

    JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

    Oral presentation

  • The anti-diabetes effect of quercetin and its metabolite isorhamnetin on glucose uptake in myotubes

    JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    JSBBA Kansai (日本農芸化学会関西支部)3rd Student Forum, Nov. 2016, English, Kobe, International conference

    Oral presentation

  • Prevention of hyperglycemia and obesity by cacao polyphenols

    ASHIDA Hitoshi, YAMASHITA Yoko

    The 3rd International Conference on Pharma and Food, Nov. 2016, English, Shizuoka, International conference

    [Invited]

    Invited oral presentation

  • Piperine induces glucose uptake through AMPK signaling pathway

    MAEDA Ayumi, YAMASHITA Yoko, YOSHIOKA Yasukiyo, AKAGAW Mitsugu, ASHIDA Hitoshi

    The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

    Oral presentation

  • Luteolin enhanced kaempferol-inhibiting expression of drug-metabolizing enzymes

    NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

    The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

    Poster presentation

  • Effect of cacao polyphenols on energy metabolism: Administration timing and clock genes

    MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

    JSBBA Kansai (日本農芸化学会関西支部)3rd Student Forum, Nov. 2016, English, Kobe, International conference

    Poster presentation

  • 黒大豆種皮由来のプロシアニジンとアントシアニンの肥満・高血糖予防効果とその作用機構

    YAMASHITA Yoko, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi

    第55回 日本栄養・食糧学会近畿支部大会, Oct. 2016, Japanese, Oosaka, Domestic conference

    Oral presentation

  • カカオポリフェノール抽出物の投与タイミングと時計遺伝子の関係

    MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

    第55回 日本栄養・食糧学会近畿支部大会, Oct. 2016, Japanese, Oosaka, Domestic conference

    Oral presentation

  • アシタバカルコンは白色脂肪細胞のベージュ化促進を介して脂質蓄積量を低下させる

    LiuYiyun, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

    第55回 日本栄養・食糧学会近畿支部大会, Oct. 2016, Japanese, Oosaka, Domestic conference

    Oral presentation

  • Preventive effects of black soybean seed coat extract on fibrosis in the liver of mice

    ASHIDA Hitoshi, RI Xiu, NAMBA FumioToshiya, TODA Toshiya, YAMASHITA Yoko

    The 9th International Conference and Exhibition on Nutraceuticals and Functional Foods 2016 (ISNFF2016), Oct. 2016, English, Florida, International conference

    Oral presentation

  • ~食品の生体機能性に関する研究に魅了されて~難消化性ポリフェノールによる糖代謝・脂質代謝促進効果

    YAMASHITA Yoko

    第22回フードサイエンスフォーラム学術集会, Sep. 2016, Japanese, Okayama, Domestic conference

    [Invited]

    Nominated symposium

  • 日本ヨモギエタノール抽出物が糖尿病モデルマウスにおよぼす影響

    TSUJI Ai, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, Hikone, Domestic conference

    Oral presentation

  • 黒大豆種皮ポリフェノールの抗酸化性を介した炎症性疾患予防効果

    YAMASHITA Yoko, RI Xiu, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi

    日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, Hikone, Domestic conference

    Oral presentation

  • ピペリンによる血糖調節作用の機構解明

    MAEDA Ayumi, YOSHIOKA Yasukiyo, YAMASHITA Yoko, AKAGAW Mitsugu, ASHIDA Hitoshi

    第22回フードサイエンスフォーラム学術集会, Sep. 2016, Japanese, Okayama, Domestic conference

    Public symposium

  • カテキン誘導体とアルブミンの結合親和性について

    IKEDA Masaki, WAKAGI-UEDA Manabu, HAYASHIBARA Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

    日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, Hikone, Domestic conference

    Oral presentation

  • 黒大豆種皮ポリフェノールの抗酸化能を介した疾病予防効果

    YAMASHITA Yoko, LI Xiu, YOSHIOKA Yasukiyo, ASHIDA Hitoshi

    第10回日本ポリフェノール学会, Aug. 2016, Japanese, Tokyo, Domestic conference

    Oral presentation

  • 黒大豆加工品中ポリフェノールの体内動態に関する分析

    NAKAMURA Asuka, Liuqing Wang, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

    第10回日本ポリフェノール学会, Aug. 2016, Japanese, Tokyo, Domestic conference

    Poster presentation

  • 筋肉細胞の糖取り込みを促進する緑豆タンパク質人工消化物由来ペプチドの単離と特定

    WANG Xin, YAMASHITA Yoko, ASHIDA Hitoshi

    日本食品科学工学会第63回大会, Aug. 2016, Japanese, Nagoya, Domestic conference

    Oral presentation

  • ピペリンによる血糖調節作用の機構解明

    MAEDA Ayumi, YOSHIOKA Yasukiyo, YAMASHITA Yoko, AKAGAW Mitsugu, ASHIDA Hitoshi

    日本食品科学工学会第63回大会, Aug. 2016, Japanese, Nagoya, Domestic conference

    Oral presentation

  • カテキンの相互作用による血清アルブミンの結合親和性に対する影響

    IKEDA Masaki, WAKAGI-UEDA Manabu, HAYASHIBARA Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

    第10回日本ポリフェノール学会, Aug. 2016, Japanese, Tokyo, Domestic conference

    Poster presentation

  • エビデンスに基づいた食による健康寿命の延伸

    YAMASHITA Yoko

    第50回若手栄養学研究者の集いサマーセミナー, Aug. 2016, Japanese, Tokyo, Domestic conference

    Public discourse

  • The effect of quercetin and its metabolite isorhamnetin on glucose uptake in myotubes

    JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

    第10回日本ポリフェノール学会, Aug. 2016, English, Tokyo, Domestic conference

    Poster presentation

  • 難消化性ポリフェノールの高血糖・肥満抑制効果に関わる新奇分子ターゲット

    YAMASHITA Yoko, MITSUHASHI Yuji, ASHIDA Hitoshi

    日本農芸化学会関西支部例会, Jul. 2016, Japanese, Oosaka, Domestic conference

    [Invited]

    Invited oral presentation

  • ケルセチンとイソラムネチンによる筋肉細胞へのグルコース取り込み促進効果

    ASHIDA Hitoshi, Hao Jiang, YOSHIOKA Yasukiyo, YAMASHITA Yoko

    日本ビタミン学会第68回大会, Jun. 2016, Japanese, Toyama, Domestic conference

    Oral presentation

  • ピペリンは骨格筋においてAMPKシグナル経路を介してグルコース取り込みを上昇させる

    MAEDA Ayumi, AKAGAW Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

    第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

    Oral presentation

  • クルクミンおよびその誘導体によるAhRを介した薬物代謝酵素の発現調節機構の解明

    NAKAI Rika, FUKUDA Syun, YAMASHITA Yoko, ASHIDA Hitoshi

    第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

    Oral presentation

  • グラブリジン含有組成物のマウスにおける筋肉増強作用機構

    YOSHIOKA Yasukiyo, YAMASHITA Yoko, KISHIDA Hideyuki, NAKAGAWA Kaku, ASHIDA Hitoshi

    第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

    Oral presentation

  • カルコンを高含有するアシタバ抽出物の肥満予防効果

    ASHIDA Hitoshi, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Norio

    第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

    Oral presentation

  • カカオポリフェノール抽出物の投与タイミングと時計遺伝子の発現量および血糖調節作用の関係

    MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

    第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

    Oral presentation

  • カカオポリフェノールの時間遺伝子に制御されたエネルギー代謝について

    YAMASHITA Yoko, MITSUHASHI Yuji, ASHIDA Hitoshi

    第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

    [Invited]

    Invited oral presentation

  • 黒大豆種皮抽出物の抗酸化能を介した肝損傷抑制効果について

    YAMASHITA Yoko, RI Xiu, TODA Toshiya, NAMBA Fumio, ASHIDA Hitoshi

    日本農芸化学会2016年度大会, Mar. 2016, Japanese, 札幌, Domestic conference

    Poster presentation

  • 筋肉細胞の糖取り込みを促進する緑豆タンパク質人工消化物由来ペプチドの単離と特定

    WANG Xin, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学若手フロンティア研究会, Dec. 2015, Japanese, 神戸, Domestic conference

    Poster presentation

  • プロシアニジン組成物の投与タイミングと時計遺伝子の発現量および血統調節作用の関係

    MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

    第12回日本カテキン学会年次学術大会, Dec. 2015, Japanese, 福岡, Domestic conference

    Poster presentation

  • ターメリックに含まれる黄色色素であるクルクミンによるAhRならびにNrf2を介した薬物代謝酵素発現調節機構の解明

    NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

    第30回 日本香辛料研究会, Dec. 2015, Japanese, 京都, Domestic conference

    Oral presentation

  • Procyanidin promotes translocation of glucose transporter 4 in muscle of mice through activation of insulin and AMPK signaling pathways

    YAMASHITA Yoko, ASHIDA Hitoshi

    2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

    Poster presentation

  • Prevention of procyanidins and cyaniding 3-glucoside on B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in hepatocytes

    ASHIDA Hitoshi, YAMASHITA Yoko, ZHANG Tianshun

    2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

    [Invited]

    Invited oral presentation

  • Piperine enhances glucose uptake through AMPK signaling pathway in skeletal muscle

    MAEDA Ayumi, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

    2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

    Poster presentation

  • Mung bean protein isolate improves glucose tolerance by enhancing glucose transporter 4 translocation and glucose uptake in skeletal muscle

    WANG Xin, YAMASHITA Yoko, TACHIBANA Nobuhiko, WANEZAKI Satoshi, KOHNO Mitsunori, ASHIDA Hitoshi

    2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

    Poster presentation

  • GLUT4を介したカテキン代謝物の糖取り込み促進作用

    NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    第12回日本カテキン学会年次学術大会, Dec. 2015, Japanese, 福岡, Domestic conference

    Poster presentation

  • 4-Hydroxyderricin and xanthoangerol from Ashitaba(Angelica keisekei) suppress differentiation of preadipocytes to adipocytes via AMPK and MAPK pathways

    ASHIDA Hitoshi, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Norio

    2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

    Poster presentation

  • Prevention of obesity by dietary flavonoids

    ASHIDA Hitoshi, YAMASHITA Yoko

    The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

    [Invited]

    Invited oral presentation

  • Pleventive effects of cacao polyphenols on hyperglycemia and obesity

    YAMASHITA Yoko, ASHIDA Hitoshi

    The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

    [Invited]

    Invited oral presentation

  • Kaempferol modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways

    NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

    The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

    Poster presentation

  • Epigallocatechin gallate and its metabolites promote Translocation of glucose transporter 4 in the plasma membrane of muscle cells as a preventive mechanism of hyperglycemia by tea

    ASHIDA Hitoshi, NAGANO Tomoya, YAMASHITA Yoko, UEDA-WAKAGIManabu

    19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

    [Invited]

    Invited oral presentation

  • Effect of catechin metabolites on glucose uptake in myotubes

    NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

    Poster presentation

  • Curcumin modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways in HepG2 cells

    NAKAI Rika, FUNATSU Keiji, YAMASHITA Yoko, ASHIDA Hitoshi

    19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

    Poster presentation

  • Catechin metabolites promote glucose uptake in myotubes

    NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

    Poster presentation

  • 施肥条件の異なる黒大豆における抗酸化成分の含量変化について

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

    Oral presentation

  • 黒豆種皮含有ポリフェノールは酸化的DNA損傷を抑制する

    YOSHIOKA Yasukiyo, RI Xiu, NAMBA Fumio, TODA Toshiya, YAMASHITA Yoko, ASHIDA Hitoshi

    日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

    Oral presentation

  • ダイオキシンが誘導する薬物代謝酵素の発現に対するルテオリンとケンフェロールの抑制効果

    NAKAI Rika, WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

    Oral presentation

  • サフラナールとピペリンは相乗的なグルコース取り込み促進効果を示すのか?

    Maeda Ayumi, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

    日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

    Oral presentation

  • Kaempferol modulates expression of phase Ⅰ and Ⅱdrug-metabolizing enzymes

    ASHIDA Hitoshi, Nakai Rika, YAMASHITA Yoko

    7th International Conference on Polyphenols and Health (ICPH2015), Oct. 2015, English, Tours, International conference

    Poster presentation

  • Epigallocatechin gallate and its metabolites promote glucose uptake through translocation of GLUT4 in muscle cells

    NAGANO Tomoya, YAMASHITA Yoko, UEDA-WAKAGIManabu, ASHIDA Hitoshi

    7th International Conference on Polyphenols and Health (ICPH2015), Oct. 2015, English, Tours, International conference

    Poster presentation

  • EGCGの筋肉細胞におけるグルコース取り込み促進作用機構の解明

    NAGANO Tomoya, UEDA-WAKAGIManabu, HAYASHIBAR Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

    日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

    Oral presentation

  • Chalcones suppress differentiation of preadiopocytes to adipocytes via AMPK and MAPK pathways

    ASHIDA Hitoshi, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Norio

    7th International Conference on Polyphenols and Health (ICPH2015), Oct. 2015, English, Tours, International conference

    Poster presentation

  • Development and application of an analysis method for procyanidins by using HPLC coupled with a Efluorescence detector

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    The 8th International Conference and Exhibition for Nutraceuticals and Functional Foods (ISNFF2015), Sep. 2015, English, 無雀, International conference

    Poster presentation

  • 黒大豆種皮抽出物による肝損傷抑制効果について

    RI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

    日本酸化ストレス学会, Jun. 2015, Japanese, 鹿児島, Domestic conference

    Poster presentation

  • 卵殻膜由来ペプチドによるによるグルコース取り込み促進効果について

    ASHIDA Hitoshi, TANAKA Yuuki, YAMASHITA Yoko, TAKENAKA Shinji

    日本食品科学工学会, 2015, Japanese, 京都, Domestic conference

    Poster presentation

  • β-コングリシニンによる筋肉細胞の分化と萎縮に及ぼす影響について

    KUBOTA Yuusuke, Tachibana Nobuhiko, WANEZAKI Satoshi, KOHNO Mitsunori, YAMASHITA Yoko, ASHIDA Hitoshi

    第28回日本動物細胞工学会2015年度大会(JAACT2015), 2015, Japanese, 仙台, Domestic conference

    Poster presentation

  • ポリフェノールの生活習慣病予防効果 -カカオポリフェノールの効果-

    YAMASHITA YOKO

    兵庫工業会特別講演会, 2015, Japanese, 神戸, Domestic conference

    [Invited]

    Nominated symposium

  • ピペリンによるグルコース取り込み促進効果の解明とその作用機構解明

    Maeda Ayumi, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

    日本食品科学工学会, 2015, Japanese, 京都, Domestic conference

    Poster presentation

  • ダイオキシンが誘導する消耗性症候群に対するルテオリンの抑制効果について

    ASHIDA Hitoshi, HARADA Kiyonari, MISHIMA Sakie, MITANI Takakazu, YAMASHITA Yoko, MATSUMURA Fumio

    The 2015 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry, 2015, Japanese, Domestic conference

    Oral presentation

  • クルクミンによるAhRならびにNrf2を介した薬物代謝酵素発現調節機構の解明

    NAKAI Rik, Funatsu Keiji, YAMASHITA Yoko, ASHIDA Hitoshi

    日本食品科学工学会, 2015, Japanese, 京都, Domestic conference

    Poster presentation

  • カテキン代謝物による筋肉細胞への糖取り込み促進効果

    NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    日本ポリフェノール学会, 2015, Japanese, 奈良, Domestic conference

    Poster presentation

  • カテキン代謝物によるグルコース取り込み促進作用について

    NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

    第28回日本動物細胞工学会2015年度大会(JAACT2015), 2015, Japanese, 仙台, Domestic conference

    Poster presentation

  • Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase

    YAMASHITA Yoko, WANG Liuqing, ASHIDA Hitoshi

    12th Asian Cogress of Nutrition, 2015, English, International conference

    Poster presentation

  • Oolong, black and pu-erh tea suppresses adiposity and hyperglycemia in mice via activation of AMP-activated protein kinase

    YAMASHITA Yoko, WANG Liuqing, ASHIDA Hitoshi

    International Symposium on Dietary Antioxidants and Oxidative Stress in Health (DAOSH2015), 2015, English, 淡路, International conference

    Poster presentation

  • Kaempferol modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways in HepG2 cells

    NAKAI Rika, KIMURA Yuki, YAMASHITA Yoko, ASHIDA Hitoshi

    12th Asian Cogress of Nutrition, 2015, English, International conference

    Poster presentation

  • Caco-2細胞に透過させたカフェインが3T3-L1細胞の分化に与える影響

    HARADA Kiyonari, YAMASHITA Yoko, ASHIDA Hitoshi

    The 2015 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry, 2015, Japanese, Domestic conference

    Oral presentation

  • Cacao polyphenol promotes energy metabolism under the circadian clock gene expressions

    MITSUHASHI Yuji, YAMASHITA Yoko, NATSUME Midori, ASHIDA Hitoshi

    12th Asian Cogress of Nutrition, 2015, English, International conference

    Poster presentation

  • Cacao extract inhibits adipogenesis through suppressing the expression of PPAR gamma and C/EBP alpha

    ASHIDA Hitoshi, MITANI Takakazu, WANG Liuqing, YAMASHITA Yoko

    12th Asian Cogress of Nutrition, 2015, English, International conference

    Oral presentation

  • 卵殻膜由来ペプチドによる血糖値上昇抑制効果

    TANAKA Yuuki, TAKENAKA Shinji, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学研究基盤センター 若手フロンティア研究会2014, 2014, Japanese, Domestic conference

    Poster presentation

  • 発酵茶が脂肪の蓄積に及ぼす効果とその作用機構

    YAMASHITA Yoko, ASHIDA Hitoshi

    The 11th annual meeting of the Japanese Society of Ctechinology, 2014, Japanese, Domestic conference

    Poster presentation

  • 発酵茶が脂肪の蓄積に及ぼす効果とその作用機構

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学研究基盤センター 若手フロンティア研究会2014, 2014, Japanese, Domestic conference

    Poster presentation

  • 脂肪細胞モデルにおいてルテオリンは TCDD が誘導する消耗性症候群を抑制する

    HARADA Kiyonari, MISHIMA Sakie, YAMASHITA Yoko, ASHIDA Hitoshi

    The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

    Poster presentation

  • 脂肪細胞の分化におけるカフェインとその代謝物の効果

    HARADA Kiyonari, YAMASHITA Yoko, ASHIDA Hitoshi

    The 2014 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry in KANSAI, 2014, Japanese, Domestic conference

    Oral presentation

  • 黒大豆種皮由来プロシアニジン高含有抽出物が肥満・高血糖に及ぼす効果について

    WATANABE Yuki, YAMASHITA Yoko, NANNBA Fumio, TODA Toshio, ASHIDA Hitoshi

    The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

    Poster presentation

  • 高感度HPLCを用いたプロシアニジン分析系の構築とその適用

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    The 2014 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry in KANSAI, 2014, Japanese, Domestic conference

    Oral presentation

  • 紅茶による筋肉細胞へのGLUT4細胞膜移行促進機構の解明

    NAGANO Tomoya, HAYASHIBAR Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学研究基盤センター 若手フロンティア研究会2014, 2014, Japanese, Domestic conference

    Poster presentation

  • 蛍光検出器付き HPLC によるプロシアニジン分析法の構築とその適用

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

    Poster presentation

  • 甘草由来グラブリジン高含有組成物が血糖調節に及ぼす効果

    YAMASHITA Yoko, KISHIDA Hidenobu, NAKAGAWA Kaku, ASHIDA Hitoshi

    The 61st Annual Meeting of the Japanese Society for Food Science and Technology, 2014, Japanese, Domestic conference

    Poster presentation

  • 甘草グラブラポリフェノールの主成分であるグラブリジンによる筋肉細胞への糖取り込み促進を介した血糖調節作用

    YAMASHITA Yoko, ASHIDA HITOSHI

    第17回日本補完代替医療学会学術集会, 2014, Japanese, Domestic conference

    [Invited]

    Invited oral presentation

  • β-コングリシニンの筋肉分化に及ぼす影響について

    KUBOTA Yusuke, TACHIBANA Nobuhiko, WANEZAKI Satoshi, KOUNO Mitsuto, YAMASHITA Yoko, ASHIDA Hitoshi

    The 53th Annual meeting of the Japan Society of Nutrition and Food Science in KINKI, 2014, Japanese, Domestic conference

    Oral presentation

  • ルテオリンがAhR-Nrf2経路を介した薬物代謝系酵素発現に与える影響

    KIMURA Yuki, JIANG Songyan, YAMASHITA Yoko, ASHIDA Hitoshi

    The 68th Annual meeting of the Japan Society of Nutrition and Food Science, 2014, Japanese, Domestic conference

    Oral presentation

  • メタボリックシンドロームの予防・改善に寄与する機能性成分の網羅的探索法と作用機構解明,ならびにそれを高含有する農作物生産のための環境創造型農業の開発

    YAMASHITA Yoko, ASHIDA Hitoshi, YASUDA Shigeru

    第3回ネイチャー・インダストリー・アワード, 2014, Japanese, Domestic conference

    [Invited]

    Invited oral presentation

  • プロシアニジンの高血糖・抗肥満予防作用について

    ASHIDA Hitoshi, YAMASHITA Yoko

    第12回日本機能性食品医用学会総会, 2014, Japanese, Domestic conference

    [Invited]

    Invited oral presentation

  • プロシアニジンのインクレチンならびにエネルギー産生上昇効果

    YAMASHITA Yoko, ASHIDA Hitoshi

    The 68th Annual meeting of the Japan Society of Nutrition and Food Science, 2014, Japanese, Domestic conference

    [Invited]

    Invited oral presentation

  • ピペリンによるグルコース取り込み促進効果の解明および作用機序の解析

    MAEDA Ayumi, AKAGAWA Mitugu, YAMASHITA Yoko, ASHIDA Hitoshi

    The 29th annual meeting of the japan society for spice and herbResearch, 2014, Japanese, Domestic conference

    Oral presentation

  • グラブリジンの筋肉細胞における高血糖抑制効果とその作用機構

    YAMASHITA Yoko, KISHIDA Hidenobu, NAKAGAWA Kaku, ASHIDA Hitoshi

    The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

    Oral presentation

  • カフェインとその代謝物が脂肪細胞分化に与える影響

    HARADA Kiyonari, YAMASHITA Yoko, ASHIDA Hitoshi

    The 61st Annual Meeting of the Japanese Society for Food Science and Technology, 2014, Japanese, Domestic conference

    Poster presentation

  • カカオ豆抽出物による脂肪細胞の分化と脂肪蓄積の抑制効果

    MITANI Takakazu, YAMASHITA Yoko, YAMAMOTO Kazunari, KAWABATA Hiromu, ASHIDA Hitoshi

    The 68th Annual meeting of the Japan Society of Nutrition and Food Science, 2014, Japanese, Domestic conference

    Oral presentation

  • カカオリカープロシアニジンの投与時間の違いがエネルギー代謝と時計遺伝子発現におよぼす効果

    光橋 雄史, MITSUHASHI Yuji, YAMASHITA Yoko, NATSUME MIDORI, ASHIDA Hitoshi

    The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

    Poster presentation

  • Luteolin Modulates Expression of Drug-metabolizing Enzymes through AhR and Nrf2 Pathways in Hepatocytes.

    KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, YAMASHITA Yoko, ASHIDA Hitoshi

    XXVIth International Conference on Polyphenols & 8th Tannin Conference (ICP2014), 2014, English, International conference

    Poster presentation

  • Glabridin suppresses hyperglycemia through promotion of glucose uptake accompanied by translocation of glucose transporter 4 in muscle.

    YAMASHITA Yoko, SAWADA Keisuke, KISHIDA Hidenobu, NAKAGAWA Kaku, ASHIDA Hitoshi

    XXVIth International Conference on Polyphenols & 8th Tannin Conference (ICP2014), 2014, English, International conference

    Poster presentation

  • Effects of luteolin on TCDD- and tert-butylhydroquinone-induced drug-metabolizing enzynes and nuclear factor-erythroid-2-related factor 2.

    ASHIDA Hitoshi, ZHANG Tianshun, KIMURA Yuki, JIANG Songyan, HARADA Kiyonari, YAMASHITA Yoko

    The 34th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN2014), 2014, English, International conference

    Poster presentation

  • Bioavailability of procyanidins in mice.

    WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

    XXVIth International Conference on Polyphenols & 8th Tannin Conference (ICP2014), 2014, English, International conference

    Poster presentation

  • 重合度の異なるカカオ由来プロシアニジンが筋肉細胞でのGLUT4膜移行を介したグルコース取り込みにおよぼす効果

    YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

    第60回日本食品科学工学会, 2013, Japanese, 立川, Domestic conference

    Oral presentation

  • 黒大豆成分の摂取による血糖値上昇抑制作用とその作用機序解明

    KURIMOTO Yuta, SHIBAYAMA Yuki, INOUE Seiya, SOGA Minoru, TAKIKAWA Masahito, ITO Chiaki, NANBA Fumio, YOSHIDA Tadashi, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

    第60回日本食品科学工学会, 2013, Japanese, 立川, Domestic conference

    Oral presentation

  • 黒大豆種皮抽出物による薬物代謝系調節能を介したベンゾピレンの毒性抑制

    KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, HE Chao, YAMASHITA Yoko, ASHIDA Hitoshi

    第67回日本栄養・食糧学会大会, 2013, Japanese, 名古屋, Domestic conference

    Oral presentation

  • 黒大豆種皮抽出物によるベンゾピレンの毒性抑制機構の解明

    KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, HE Chao, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学若手フロンティア研究会2013, 2013, Japanese, 神戸, Domestic conference

    Poster presentation

  • プロシアニジン単回経口投与マウスにおける体内動態に関する研究

    WANG Linqing, YAMASHITA Yoko, ASHIDA Hitoshi

    神戸大学若手フロンティア研究会2013, 2013, Japanese, 神戸, Domestic conference

    Poster presentation

  • プロシアニジンのマウスにおける体内動態に関する研究

    WANG Linqing, YAMASHITA Yoko, ASHIDA Hitoshi

    第18回日本フードファクター学会 学術集会(JSoFF2013), 2013, Japanese, 東京, Domestic conference

    Oral presentation

  • プロシアニジンのインクレチン効果を介した食後高血糖調節作用

    YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

    第67回日本栄養・食糧学会大会, 2013, Japanese, 名古屋, Domestic conference

    Oral presentation

  • プロシアニジンによる血糖値上昇抑制効果とその作用機構解明

    YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

    日本栄養・食糧学会第52回近畿支部大会, 2013, Japanese, 彦根, Domestic conference

    Oral presentation

  • カカオ由来プロシアニジンによるインクレチン効果を介した血糖調整作用

    YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

    第18回日本フードファクター学会 学術集会(JSoFF2013), 2013, Japanese, 東京, Domestic conference

    Oral presentation

  • カカオポリフェノールの高血糖・肥満抑制効果とその作用機構

    YAMASHITA Yoko, ASHIDA Hitoshi

    第5回食品薬学シンポジウム, 2013, Japanese, 京都, Domestic conference

    Oral presentation

  • カカオポリフェノールによる肥満抑制について

    HARADA Kiyonari, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Kazuya, KAWABATA Hiroshi, ASHIDA Hitoshi

    第60回日本食品科学工学会, 2013, Japanese, 立川, Domestic conference

    Oral presentation

  • Suppression of adipogenesis-relating factors by fermented tea

    TANAKA Yuki, YAMASHITA Yoko, ASHIDA Hitoshi

    The 5th International conference on O-CHA (Tea) Culture and Science, 2013, English, Shizuoka, Japan, International conference

    Poster presentation

  • Prevention mechanism of glucose intolerance and obesity by cacao liquor procyanidin extract

    ASHIDA Hitoshi, YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori

    The IUNS 20th International Congress of Nutrition (ICN2013), 2013, English, Granada, Spain, International conference

    Poster presentation

  • Intake of tea prevents postprandial hyperglycemia by promoting GLUT4 translocation in skeletal muscle

    YAMASHITA Yoko, ASHIDA Hitoshi

    The 5th International conference on O-CHA (Tea) Culture and Science, 2013, English, Shizuoka, Japan, International conference

    Poster presentation

  • Fermented tea improves glucose intolerance in mice by enhancing translocation of glucose transporter 4 in skeletal muscle

    YAMASHITA Yoko, WANG Lihua, ZHANG Tianshun, NAKAMURA Toshiuki, ASHIDA Hitoshi

    VIth International Conference on Polyphenol and Health, 2013, English, Buenos Aires, Argentina, International conference

    Poster presentation

  • Effects of flavonoids on the expression system of drug-metabolizing enzymes

    ASHIDA Hitoshi, ZHANG Tianshun, JIANG Songyan, HE Chao, KIMURA Yuki, YAMASHITA Yoko

    Antioxidants and redox process in health – Bilateral Meeting Japan-Brazil, 2013, English, San Pauro, Brazil, International conference

    [Invited]

    Invited oral presentation

  • Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in hepatocytes

    ASHIDA Hitoshi, ZHANG Tianshun, JIANG Songyan, HE Chao, Yuki Kimura, YAMASHITA Yoko

    VIth International Conference on Polyphenol and Health, 2013, English, Buenos Aires, Argentina, International conference

    Oral presentation

  • Black soybean seed coat extract inhibits genotoxicity induced by benzo[a]pyrene through modulating of drug-metabolizing enzymes

    KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, HE Chao, YAMASHITA Yoko, ASHIDA Hitoshi

    Antioxidants and redox process in health – Bilateral Meeting Japan-Brazil, 2013, English, San Pauro, Brazil, International conference

    Poster presentation

  • Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice

    KURIMOTO Yuta, SHIBAYAMA Yuki, INOUE Seiya, SOGA Minoru, TAKIKAWA Masahito, ITO Chiaki, NANBA Fumio, YOSHIDA Tadashi, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

    7th International Work shop on Anthocyanins, 2013, English, Port, Portogal, International conference

    Oral presentation

  • 大豆たん白質由来ペプチドによる筋肉細胞への糖取込み活性促進作用とその作用機序の解明

    SAWADA Keisuke, 山下 陽子YAMASHITA Yoko, ASHIDA Hitoshi

    不二たん白質研究振興財団第15回研究報告会, 2012, Japanese, 仙台, Domestic conference

    Nominated symposium

  • 黒大豆成分の血糖値上昇抑制作用とその作用機序

    KURIMOTO Yuta, TAKIKAWA Masahito, SHIBAYAMA Yuki, SOGA Minoru, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

    第66回日本栄養・食糧学会大会, 2012, Japanese, 仙台, Domestic conference

    Oral presentation

  • 黒大豆成分の血糖値上昇抑制作用とその作用機構の解明

    KURIMOTO Yuta, TAKIKAWA Masahito, SHIBAYAMA Yuki, SOGA Minoru, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

    日本農芸化学会2012年度大会, 2012, Japanese, 京都, Domestic conference

    Oral presentation

  • 酵素処理イソケルシトリンおよびケルセチンがGLUT4細胞膜移行に及ぼす影響

    HORIUCHI Yuko, YAMASHITA Yoko, OGAWA Rui, ASHIDA Hitoshi

    第66回日本栄養・食糧学会大会, 2012, Japanese, 仙台, Domestic conference

    Oral presentation

  • 酵素処理イソクエルシトリンおよびケルセチンによる食餌誘導性高血糖の予防効果

    HORIUCHI Yuko, YAMASHITA Yoko, ASHIDA Hitoshi

    日本栄養・食糧学会第51回近畿支部大会, 2012, Japanese, 宝塚, Domestic conference

    Oral presentation

  • ロシアニジンの食餌由来高血糖および肥満予防改善効果

    YAMASHITA Yoko, ASHIDA Hitoshi

    第17回日本フードファクター学会学術集会・第9回日本カテキン学会総会合同大会, 2012, Japanese, 静岡, Domestic conference

    Oral presentation

  • カカオ由来プロシアニジン高含有組成物の肥満予防効果

    YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

    日本農芸化学会2012年度大会, 2012, Japanese, 京都, Domestic conference

    Oral presentation

  • 放射化分析による緑茶抽出物を摂取したマウス体毛中の元素分析

    KAWASE Masaya, SAITO Tadashi, NAKANO Yukihiro, YAMASHITA Yoko, IMADA Sayuri, ASHIDA Hitoshi

    京都大学原子炉利用者講演会, 2011, Japanese, Domestic conference

    Oral presentation

  • 黒大豆種皮抽出物の肥満抑制作用機構の解明

    YAMASHITA Yoko, KANAMOTO Yuki, NAMBA Fumio, YOSHIDA Tadashi, TSUDA Takanori, NAKAMURA-TSURUTA Sachiko, ASHIDA Hitoshi

    日本農芸化学会2011年度大会, 2011, Japanese, Domestic conference

    Oral presentation

  • 酵素処理イソクエルシトリン (EMIQ)および ケルセチンの筋肉細胞へのグルコース取込み促進効果について

    SHIRAO Takeshi, YAMASHITA Yoko, UEDA Manabu, SAWADA Keisuke, ASHIDA Hitoshi

    食品科学工学会第58回大会, 2011, Japanese, Domestic conference

    Oral presentation

  • カカオポリフェノールは筋肉細胞でのGLUT4膜移行の促進を介して食後高血糖を低下させる

    YAMASHITA Yoko, UEDA Manabu, OGAWA Rui, NATSUME Midori, ASHIDA Hitoshi

    第65回日本栄養・食糧学大会, 2011, Japanese, Domestic conference

    Oral presentation

  • エピカテキン重合体であるプロシアニジンを高含有する組成物の肥満予防効果

    YAMASHITA Yoko, ASHIDA Hitoshi

    第8回日本カテキン学会, 2011, Japanese, Domestic conference

    Oral presentation

  • Quercetin and enzymatically modified isoquercitrin stimulate glucose uptake activity in skeletal muscle

    ASHIDA Hitoshi, YAMASHITA Yoko, SHIRAO Takeshi, SAWADA Keisuke, UEDA Manabu

    5th International conference on Polyphenols and Health, 2011, English, International conference

    Poster presentation

  • Polyphenol-rich black soybean seed coat extract prevents hyperglycemia, insulin resistance and obesity in high-fat diet-fed C57BL/6J mice

    YAMASHITA Yoko, TSUDA Takanori, YOSHIDA Tadashi, TODA Toshiya, ASHIDA Hitoshi

    XI Asian Congress of Nutrition (ACN2011), 2011, English, International conference

    Poster presentation

  • Effect of green tea extract on elemental contents in mice liver

    KAWASE Masaya, YAMASHITA Yoko, IMADA Sayuri, ASHIDA Hitoshi

    5th International conference on Polyphenols and Health, 2011, English, International conference

    Poster presentation

  • Cacao liquor procyanidin extract suppresses hyperglycemia through promotion of glucose uptake accompanied by translocation of glucose transporter 4 in muscle

    YAMASHITA Yoko, NATSUME Midori, ASHIDA Hitoshi

    2011 International Conference on Food Factors (ICoFF2011), 2011, English, International conference

    [Invited]

    Invited oral presentation

  • Cacao liquor procyanidinextract prevents hyperglycemia in mice

    ASHIDA Hitoshi, YAMASHITA Yoko, NATSUME Midori

    5th International conference on Polyphenols and Health, 2011, English, International conference

    Oral presentation

  • Black soybean polyphenol prevents hyperglycemia, insulin resistance and obesity

    ASHIDA Hitoshi, YAMASHITA Yoko, TSUDA Takanori, YOSHIDA Tadashi, TODA Toshiya

    International Society for Nutraceuticals &Functional Foods, 2011 Annual conference (ISNFF2011), 2011, English, International conference

    [Invited]

    Invited oral presentation

  • カカオポリフェノール抽出物の筋肉細胞へのグルコース取り込み促進効果について

    YAMASHITA Yoko, UEDA Manabu, NATSUME Midori, ASHIDA Hitoshi

    日本食品科学工学会第57回大会, 2010, Japanese, Domestic conference

    Oral presentation

  • Effects of enzymatically synthesized glycogen on lipid methabolism in vivo

    YAMASHITA Yoko, OGAWA Rui, NAKAMURA-TSURUTA Sachiko, FURUYASHIKI Takashi, TAKATA Hiroki, ASHIDA Hitoshi

    PACIFICHEM2010(環太平洋国際化学会議), 2010, English, Domestic conference

    Poster presentation

  • Cacao polyphenol extract promotes glucose uptake in muscle cells

    YAMASHITA Yoko, UEDA Manabu, NATSUME Midori, ASHIDA Hitoshi

    The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT 2010), 2010, English, International conference

    Poster presentation

Research Projects